LOCUS       DAJTYI010000001       110708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYI010000001 DAJTYI010000000
VERSION     DAJTYI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 110708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            68..2353
                     /gene="nrdA"
                     /locus_tag="OO564_000001"
     CDS             68..2353
                     /gene="nrdA"
                     /locus_tag="OO564_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6651279.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPARFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2587..3717
                     /gene="nrdB"
                     /locus_tag="OO564_000002"
     CDS             2587..3717
                     /gene="nrdB"
                     /locus_tag="OO564_000002"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6651280.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3717..3971
                     /gene="yfaE"
                     /locus_tag="OO564_000003"
     CDS             3717..3971
                     /gene="yfaE"
                     /locus_tag="OO564_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6651281.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4025..4675)
                     /gene="inaA"
                     /locus_tag="OO564_000004"
     CDS             complement(4025..4675)
                     /gene="inaA"
                     /locus_tag="OO564_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6651282.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            4839..5081
                     /locus_tag="OO564_000005"
                     /pseudo
     CDS             4839..5081
                     /locus_tag="OO564_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5123..6199)
                     /gene="glpQ"
                     /locus_tag="OO564_000006"
     CDS             complement(5123..6199)
                     /gene="glpQ"
                     /locus_tag="OO564_000006"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6651283.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6204..7562)
                     /gene="glpT"
                     /locus_tag="OO564_000007"
     CDS             complement(6204..7562)
                     /gene="glpT"
                     /locus_tag="OO564_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6651284.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            7835..9463
                     /gene="glpA"
                     /locus_tag="OO564_000008"
     CDS             7835..9463
                     /gene="glpA"
                     /locus_tag="OO564_000008"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6651285.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9453..10712
                     /gene="glpB"
                     /locus_tag="OO564_000009"
     CDS             9453..10712
                     /gene="glpB"
                     /locus_tag="OO564_000009"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6651286.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            10709..11899
                     /gene="glpC"
                     /locus_tag="OO564_000010"
     CDS             10709..11899
                     /gene="glpC"
                     /locus_tag="OO564_000010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6651287.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12092..12991
                     /locus_tag="OO564_000011"
     CDS             12092..12991
                     /locus_tag="OO564_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6651288.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            12995..13231
                     /locus_tag="OO564_000012"
                     /pseudo
     CDS             12995..13231
                     /locus_tag="OO564_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13272..13742)
                     /locus_tag="OO564_000013"
     CDS             complement(13272..13742)
                     /locus_tag="OO564_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6651289.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(13797..14999)
                     /locus_tag="OO564_000014"
     CDS             complement(13797..14999)
                     /locus_tag="OO564_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6651290.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15086..15628)
                     /locus_tag="OO564_000015"
     CDS             complement(15086..15628)
                     /locus_tag="OO564_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651291.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            15907..16332
                     /gene="nudI"
                     /locus_tag="OO564_000016"
     CDS             15907..16332
                     /gene="nudI"
                     /locus_tag="OO564_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6651292.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16371..16973)
                     /locus_tag="OO564_000017"
     CDS             complement(16371..16973)
                     /locus_tag="OO564_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6651293.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17281..18420
                     /gene="arnB"
                     /locus_tag="OO564_000018"
     CDS             17281..18420
                     /gene="arnB"
                     /locus_tag="OO564_000018"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6651294.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18424..19392
                     /gene="arnC"
                     /locus_tag="OO564_000019"
     CDS             18424..19392
                     /gene="arnC"
                     /locus_tag="OO564_000019"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6651295.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19392..21374
                     /gene="arnA"
                     /locus_tag="OO564_000020"
     CDS             19392..21374
                     /gene="arnA"
                     /locus_tag="OO564_000020"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6651296.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21371..22261
                     /locus_tag="OO564_000021"
     CDS             21371..22261
                     /locus_tag="OO564_000021"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6651297.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22261..23913
                     /gene="arnT"
                     /locus_tag="OO564_000022"
     CDS             22261..23913
                     /gene="arnT"
                     /locus_tag="OO564_000022"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6651298.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            23910..24245
                     /gene="arnE"
                     /locus_tag="OO564_000023"
     CDS             23910..24245
                     /gene="arnE"
                     /locus_tag="OO564_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6651299.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24245..24631
                     /gene="arnF"
                     /locus_tag="OO564_000024"
     CDS             24245..24631
                     /gene="arnF"
                     /locus_tag="OO564_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6651300.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24625..24897)
                     /gene="pmrD"
                     /locus_tag="OO564_000025"
     CDS             complement(24625..24897)
                     /gene="pmrD"
                     /locus_tag="OO564_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6651301.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25002..26357)
                     /gene="menE"
                     /locus_tag="OO564_000026"
     CDS             complement(25002..26357)
                     /gene="menE"
                     /locus_tag="OO564_000026"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6651302.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26354..27316)
                     /gene="menC"
                     /locus_tag="OO564_000027"
     CDS             complement(26354..27316)
                     /gene="menC"
                     /locus_tag="OO564_000027"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6651303.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27316..28173)
                     /gene="menB"
                     /locus_tag="OO564_000028"
     CDS             complement(27316..28173)
                     /gene="menB"
                     /locus_tag="OO564_000028"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6651304.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28188..28946)
                     /gene="menH"
                     /locus_tag="OO564_000029"
     CDS             complement(28188..28946)
                     /gene="menH"
                     /locus_tag="OO564_000029"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6651305.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(28943..30613)
                     /gene="menD"
                     /locus_tag="OO564_000030"
     CDS             complement(28943..30613)
                     /gene="menD"
                     /locus_tag="OO564_000030"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6651306.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30702..31997)
                     /gene="menF"
                     /locus_tag="OO564_000031"
     CDS             complement(30702..31997)
                     /gene="menF"
                     /locus_tag="OO564_000031"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6651307.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32076..32381)
                     /gene="elaB"
                     /locus_tag="OO564_000032"
     CDS             complement(32076..32381)
                     /gene="elaB"
                     /locus_tag="OO564_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6651308.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32436..32897)
                     /locus_tag="OO564_000033"
     CDS             complement(32436..32897)
                     /locus_tag="OO564_000033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6651309.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            32962..33879
                     /gene="rbn"
                     /locus_tag="OO564_000034"
     CDS             32962..33879
                     /gene="rbn"
                     /locus_tag="OO564_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6651310.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34067..35287
                     /gene="elaD"
                     /locus_tag="OO564_000035"
     CDS             34067..35287
                     /gene="elaD"
                     /locus_tag="OO564_000035"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6651311.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            35707..36322
                     /locus_tag="OO564_000036"
                     /pseudo
     CDS             35707..36322
                     /locus_tag="OO564_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36425..36928
                     /locus_tag="OO564_000037"
     CDS             36425..36928
                     /locus_tag="OO564_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR6651312.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(36995..38452)
                     /gene="nuoN"
                     /locus_tag="OO564_000038"
     CDS             complement(36995..38452)
                     /gene="nuoN"
                     /locus_tag="OO564_000038"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6651313.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38459..39988)
                     /gene="nuoM"
                     /locus_tag="OO564_000039"
     CDS             complement(38459..39988)
                     /gene="nuoM"
                     /locus_tag="OO564_000039"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6651314.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40152..41993)
                     /gene="nuoL"
                     /locus_tag="OO564_000040"
     CDS             complement(40152..41993)
                     /gene="nuoL"
                     /locus_tag="OO564_000040"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6651315.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(41990..42292)
                     /gene="nuoK"
                     /locus_tag="OO564_000041"
     CDS             complement(41990..42292)
                     /gene="nuoK"
                     /locus_tag="OO564_000041"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6651316.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42289..42843)
                     /gene="nuoJ"
                     /locus_tag="OO564_000042"
     CDS             complement(42289..42843)
                     /gene="nuoJ"
                     /locus_tag="OO564_000042"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6651317.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(42855..43397)
                     /gene="nuoI"
                     /locus_tag="OO564_000043"
     CDS             complement(42855..43397)
                     /gene="nuoI"
                     /locus_tag="OO564_000043"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6651318.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43412..44389)
                     /gene="nuoH"
                     /locus_tag="OO564_000044"
     CDS             complement(43412..44389)
                     /gene="nuoH"
                     /locus_tag="OO564_000044"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6651319.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44386..47112)
                     /gene="nuoG"
                     /locus_tag="OO564_000045"
     CDS             complement(44386..47112)
                     /gene="nuoG"
                     /locus_tag="OO564_000045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6651320.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47165..48502)
                     /gene="nuoF"
                     /locus_tag="OO564_000046"
     CDS             complement(47165..48502)
                     /gene="nuoF"
                     /locus_tag="OO564_000046"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6651321.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(48499..48999)
                     /gene="nuoE"
                     /locus_tag="OO564_000047"
     CDS             complement(48499..48999)
                     /gene="nuoE"
                     /locus_tag="OO564_000047"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6651322.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49002..50804)
                     /gene="nuoC"
                     /locus_tag="OO564_000048"
     CDS             complement(49002..50804)
                     /gene="nuoC"
                     /locus_tag="OO564_000048"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6651323.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(50898..51560)
                     /gene="nuoB"
                     /locus_tag="OO564_000049"
     CDS             complement(50898..51560)
                     /gene="nuoB"
                     /locus_tag="OO564_000049"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6651324.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(51576..52019)
                     /gene="nuoA"
                     /locus_tag="OO564_000050"
     CDS             complement(51576..52019)
                     /gene="nuoA"
                     /locus_tag="OO564_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6651325.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(52650..53588)
                     /gene="lrhA"
                     /locus_tag="OO564_000051"
     CDS             complement(52650..53588)
                     /gene="lrhA"
                     /locus_tag="OO564_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6651326.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            54532..55749
                     /gene="alaA"
                     /locus_tag="OO564_000052"
     CDS             54532..55749
                     /gene="alaA"
                     /locus_tag="OO564_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6651327.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            55833..56432
                     /gene="yfbR"
                     /locus_tag="OO564_000053"
     CDS             55833..56432
                     /gene="yfbR"
                     /locus_tag="OO564_000053"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6651328.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(56491..58323)
                     /locus_tag="OO564_000054"
     CDS             complement(56491..58323)
                     /locus_tag="OO564_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6651329.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58410..59060)
                     /gene="hxpA"
                     /locus_tag="OO564_000055"
     CDS             complement(58410..59060)
                     /gene="hxpA"
                     /locus_tag="OO564_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6651330.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59071..59565)
                     /locus_tag="OO564_000056"
     CDS             complement(59071..59565)
                     /locus_tag="OO564_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6651331.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(59648..60103)
                     /locus_tag="OO564_000057"
     CDS             complement(59648..60103)
                     /locus_tag="OO564_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6651332.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60441..61643
                     /gene="ackA"
                     /locus_tag="OO564_000058"
     CDS             60441..61643
                     /gene="ackA"
                     /locus_tag="OO564_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6651333.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            61718..63862
                     /gene="pta"
                     /locus_tag="OO564_000059"
     CDS             61718..63862
                     /gene="pta"
                     /locus_tag="OO564_000059"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6651334.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64052..65572
                     /gene="yfcC"
                     /locus_tag="OO564_000060"
     CDS             64052..65572
                     /gene="yfcC"
                     /locus_tag="OO564_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6651335.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(65605..66147)
                     /gene="yfcD"
                     /locus_tag="OO564_000061"
     CDS             complement(65605..66147)
                     /gene="yfcD"
                     /locus_tag="OO564_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6651336.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66205..66756)
                     /gene="yfcE"
                     /locus_tag="OO564_000062"
     CDS             complement(66205..66756)
                     /gene="yfcE"
                     /locus_tag="OO564_000062"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6651337.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(66812..67456)
                     /gene="yfcF"
                     /locus_tag="OO564_000063"
     CDS             complement(66812..67456)
                     /gene="yfcF"
                     /locus_tag="OO564_000063"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6651338.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            67592..68239
                     /gene="yfcG"
                     /locus_tag="OO564_000064"
     CDS             67592..68239
                     /gene="yfcG"
                     /locus_tag="OO564_000064"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6651339.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68296..68658
                     /gene="folX"
                     /locus_tag="OO564_000065"
     CDS             68296..68658
                     /gene="folX"
                     /locus_tag="OO564_000065"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6651340.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            68679..69572
                     /locus_tag="OO564_000066"
     CDS             68679..69572
                     /locus_tag="OO564_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6651341.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(69620..70510)
                     /gene="rpnB"
                     /locus_tag="OO564_000067"
     CDS             complement(69620..70510)
                     /gene="rpnB"
                     /locus_tag="OO564_000067"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6651342.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(70707..71480)
                     /gene="hisP"
                     /locus_tag="OO564_000068"
     CDS             complement(70707..71480)
                     /gene="hisP"
                     /locus_tag="OO564_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6651343.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(71488..72204)
                     /locus_tag="OO564_000069"
     CDS             complement(71488..72204)
                     /locus_tag="OO564_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6651344.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72201..72887)
                     /gene="hisQ"
                     /locus_tag="OO564_000070"
     CDS             complement(72201..72887)
                     /gene="hisQ"
                     /locus_tag="OO564_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6651345.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(72977..73759)
                     /gene="hisJ"
                     /locus_tag="OO564_000071"
     CDS             complement(72977..73759)
                     /gene="hisJ"
                     /locus_tag="OO564_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6651346.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(73980..74762)
                     /gene="argT"
                     /locus_tag="OO564_000072"
     CDS             complement(73980..74762)
                     /gene="argT"
                     /locus_tag="OO564_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6651347.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75028..75597)
                     /gene="ubiX"
                     /locus_tag="OO564_000073"
     CDS             complement(75028..75597)
                     /gene="ubiX"
                     /locus_tag="OO564_000073"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6651348.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(75692..77209)
                     /gene="purF"
                     /locus_tag="OO564_000074"
     CDS             complement(75692..77209)
                     /gene="purF"
                     /locus_tag="OO564_000074"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6651349.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77246..77734)
                     /gene="cvpA"
                     /locus_tag="OO564_000075"
     CDS             complement(77246..77734)
                     /gene="cvpA"
                     /locus_tag="OO564_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6651350.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(77993..78655)
                     /gene="dedD"
                     /locus_tag="OO564_000076"
     CDS             complement(77993..78655)
                     /gene="dedD"
                     /locus_tag="OO564_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6651351.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(78645..79913)
                     /gene="folC"
                     /locus_tag="OO564_000077"
     CDS             complement(78645..79913)
                     /gene="folC"
                     /locus_tag="OO564_000077"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6651352.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(79983..80897)
                     /gene="accD"
                     /locus_tag="OO564_000078"
     CDS             complement(79983..80897)
                     /gene="accD"
                     /locus_tag="OO564_000078"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6651353.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81053..81712)
                     /locus_tag="OO564_000079"
     CDS             complement(81053..81712)
                     /locus_tag="OO564_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6651354.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(81795..82607)
                     /gene="truA"
                     /locus_tag="OO564_000080"
     CDS             complement(81795..82607)
                     /gene="truA"
                     /locus_tag="OO564_000080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6651355.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(82607..83620)
                     /locus_tag="OO564_000081"
     CDS             complement(82607..83620)
                     /locus_tag="OO564_000081"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6651356.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(83686..84822)
                     /gene="pdxB"
                     /locus_tag="OO564_000082"
     CDS             complement(83686..84822)
                     /gene="pdxB"
                     /locus_tag="OO564_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6651357.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            84921..85916
                     /gene="flk"
                     /locus_tag="OO564_000083"
     CDS             84921..85916
                     /gene="flk"
                     /locus_tag="OO564_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6651358.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(85913..87091)
                     /locus_tag="OO564_000084"
     CDS             complement(85913..87091)
                     /locus_tag="OO564_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6651359.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87366..88586)
                     /gene="fabB"
                     /locus_tag="OO564_000085"
     CDS             complement(87366..88586)
                     /gene="fabB"
                     /locus_tag="OO564_000085"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6651360.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            88745..90751
                     /gene="mnmC"
                     /locus_tag="OO564_000086"
     CDS             88745..90751
                     /gene="mnmC"
                     /locus_tag="OO564_000086"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6651361.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(90872..91150)
                     /locus_tag="OO564_000087"
     CDS             complement(90872..91150)
                     /locus_tag="OO564_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6651362.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91184..91732)
                     /gene="epmC"
                     /locus_tag="OO564_000088"
     CDS             complement(91184..91732)
                     /gene="epmC"
                     /locus_tag="OO564_000088"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6651363.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(91732..92541)
                     /locus_tag="OO564_000089"
     CDS             complement(91732..92541)
                     /locus_tag="OO564_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6651364.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(92541..93365)
                     /gene="mepA"
                     /locus_tag="OO564_000090"
     CDS             complement(92541..93365)
                     /gene="mepA"
                     /locus_tag="OO564_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6651365.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93369..94454)
                     /gene="aroC"
                     /locus_tag="OO564_000091"
     CDS             complement(93369..94454)
                     /gene="aroC"
                     /locus_tag="OO564_000091"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6651366.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(94489..95421)
                     /gene="prmB"
                     /locus_tag="OO564_000092"
     CDS             complement(94489..95421)
                     /gene="prmB"
                     /locus_tag="OO564_000092"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6651367.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            95587..96138
                     /gene="smrB"
                     /locus_tag="OO564_000093"
     CDS             95587..96138
                     /gene="smrB"
                     /locus_tag="OO564_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6651368.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96242..97132)
                     /locus_tag="OO564_000094"
     CDS             complement(96242..97132)
                     /locus_tag="OO564_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6651369.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97197..97643)
                     /locus_tag="OO564_000095"
     CDS             complement(97197..97643)
                     /locus_tag="OO564_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6651370.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(97640..98110)
                     /locus_tag="OO564_000096"
     CDS             complement(97640..98110)
                     /locus_tag="OO564_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6651371.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98107..98655)
                     /locus_tag="OO564_000097"
     CDS             complement(98107..98655)
                     /locus_tag="OO564_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6651372.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(98660..99382)
                     /locus_tag="OO564_000098"
     CDS             complement(98660..99382)
                     /locus_tag="OO564_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6651373.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99402..100124)
                     /locus_tag="OO564_000099"
     CDS             complement(99402..100124)
                     /locus_tag="OO564_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6651374.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100184..102043)
                     /locus_tag="OO564_000100"
     CDS             complement(100184..102043)
                     /locus_tag="OO564_000100"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR6651375.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102125..102688)
                     /locus_tag="OO564_000101"
     CDS             complement(102125..102688)
                     /locus_tag="OO564_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6651376.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103372..103857)
                     /gene="sixA"
                     /locus_tag="OO564_000102"
     CDS             complement(103372..103857)
                     /gene="sixA"
                     /locus_tag="OO564_000102"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6651377.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104060..106204)
                     /gene="fadJ"
                     /locus_tag="OO564_000103"
     CDS             complement(104060..106204)
                     /gene="fadJ"
                     /locus_tag="OO564_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6651378.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106204..107514)
                     /gene="fadI"
                     /locus_tag="OO564_000104"
     CDS             complement(106204..107514)
                     /gene="fadI"
                     /locus_tag="OO564_000104"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6651379.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(107694..107978)
                     /locus_tag="OO564_000105"
     CDS             complement(107694..107978)
                     /locus_tag="OO564_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6651380.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108350..109690
                     /gene="fadL"
                     /locus_tag="OO564_000106"
     CDS             108350..109690
                     /gene="fadL"
                     /locus_tag="OO564_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6651381.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110056..>110708
                     /locus_tag="OO564_000107"
     CDS             110056..>110708
                     /locus_tag="OO564_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651382.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRING"
BASE COUNT        27644 a        29694 c        27305 g        26065 t
ORIGIN      
        1 ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa aaacaggtac
       61 gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag agcgcatcaa
      121 tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata acgtttcgat
      181 ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga cctctgacat
      241 ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc cggattatca
      301 gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg gccagtttga
      361 gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat acgataatca
      421 tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta tcgatcacga
      481 ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat atctggtaca
      541 gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc ttgttgccgc
      601 gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc gtttttacga
      661 cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg tgcgtacccc
      721 gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg attccatcaa
      781 cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg gcatcaacgc
      841 cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc ataccggctg
      901 tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg gcggtgtgcg
      961 cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa gcctgctggt
     1021 gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact acggggtaca
     1081 aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc tgttcagccc
     1141 gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt ttgaacgcct
     1201 gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag ccgttgagct
     1261 gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc agaacgttga
     1321 ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc agtctaacct
     1381 gtgcctggaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg agaacggtga
     1441 aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc tggatgaact
     1501 ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt atcaggatta
     1561 cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta ttggtgtgat
     1621 taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca gcgccaacaa
     1681 cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt ctaatgagct
     1741 ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga aaggtatcct
     1801 gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc tgcattacga
     1861 ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca cgctttctgc
     1921 cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta ttgaaccgcc
     1981 gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg tggtgccgga
     2041 ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta acgatggcta
     2101 cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg ctaacaccaa
     2161 ctacgatccg gcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc tgaaagacct
     2221 gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc gtgacggcgc
     2281 tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg aaagcggcgc
     2341 atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg gcctacggct
     2401 cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg ccggatgcag
     2461 cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgccagcgt
     2521 cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc ccaacaggac
     2581 acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa agaaccgatg
     2641 ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga catcttcgaa
     2701 aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga cgtctcccgc
     2761 gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat cagcaacctg
     2821 aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc gctattgccg
     2881 cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc agaaacgatt
     2941 cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc tgttgtgttt
     3001 gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc cagctattac
     3061 gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac ccacaccgtt
     3121 aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg
     3181 atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg ttcttttgcc
     3241 tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat tgcccgcgat
     3301 gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag cggcgcggat
     3361 gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga cctgtttgtc
     3421 caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg ttcgatgatt
     3481 ggtctgaata aagatattct ctgccaatac gttgaataca tcaccaatat ccgcatgcag
     3541 gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg gatcaacacc
     3601 tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg
     3661 gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca gctctgatgg
     3721 cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc
     3781 ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc gaaggttact
     3841 gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc gaaccgttag
     3901 cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa
     3961 tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat ctggcctaca
     4021 acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta gggatcggct
     4081 ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga cgcaagcggc
     4141 gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt ttcacataga
     4201 tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc ttaaacgcca
     4261 gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat actgcatgcc
     4321 gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc agcaacgcgc
     4381 gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg atgacaccgg
     4441 cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg ccgaacggat
     4501 aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt ttgccgttgc
     4561 gctcgacgca ttgcaccctg ctcataccat tacggcgata gttaggctct tcaacccagt
     4621 cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact gccataccct
     4681 atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagc aaaatcttaa
     4741 tgaaacgtgt cgtattccgc tacaggtgtg acaccgcttt tgcccttcat ttgcagcgca
     4801 tagattccac cagccactac cactaatagt gcgcccagca gataaaatgt gaatttaatc
     4861 agtcggatac agcgatgaat tttattcgac agggattagg catcgcctta caaccagagt
     4921 taacgctgaa aagcattgca ggtgaattgt gttccgttcc tcacgaacca accttctatc
     4981 gacagatttc gttgctgact aaagaaaagc cggtagaagg cagtccactg tttttactac
     5041 aaatgtgcat ggaacaatta gtggcgattg gaaaaatttg atatgagaca ggatggcgca
     5101 tgaacgccat cctgcagtaa cattactctt tattaagaaa ttttactgcc ttatcaggga
     5161 aatcagtaaa cagcccattt acacccgctt tgttatacag agcatcatat aactgattca
     5221 catcaggagt gtattcaggc agtttatctg accgcacggt ataaggatgc actaccagtt
     5281 tattctgctg agcatcttgc accatgccag tgagtttgat attacccggc tgcgatgtct
     5341 cctcaatcaa catatggtaa tccggaccaa taccatctgc atattccgcc acctgtttca
     5401 tggcacccgg cttaaacatc cagtcgtagt tgtaattaac ccagcttcca tccggctgtt
     5461 tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg agctccatgc
     5521 ccattttggg ttccagctca ttcttaatac gcttcagctc atcagcatca aaacattgca
     5581 aataaacttt atcgtcttta ccggtgtaac catatttctt cagcacttcc agcgtttttg
     5641 ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct ggatagatac
     5701 cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct tcttcaaagg
     5761 tgtgcacccg gaagtcggac ttacccattg ggaaacgccc cggataagtc tgtacttttt
     5821 taccgttttc aatatcgaaa ccttcggtaa atttcagcga cttaatttca tccagcgtga
     5881 aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga tcggcaacat
     5941 cagtaacacg atcgagataa tggtcatgca gaacaaccag atggtcgtct ttggtcatca
     6001 ccaaatcctg ctccagataa tccgctccct gcgcatacgc catcgctttt gctggcagcg
     6061 tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt tcgttgctgt
     6121 ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc atgctaaggt
     6181 ttttcagctt caatttcatg ccattagcct ccgttgcgtt tttgcagtaa ttgttcatgg
     6241 cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat gctgccgcca
     6301 atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc aacaatcgcg
     6361 ctcgccgcca ccgaaccgcc caggtaacca aacagcccgg taaagcccgc tgccgtacct
     6421 gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac aggaccgtag
     6481 atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc tgccgggttc
     6541 atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaagacgcc ggttgcccca
     6601 cggttaccac ggaagacttt gtccgacatc cagccgcaca gcagggtgcc cggaataccc
     6661 gcatactcat agaagaagta ggcccaggag gatttatcca gcgcgaaatg cttaacctct
     6721 ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac gaacacgttg
     6781 gcgatggcga tgtaccacag cagtttgttc ggcagcacgt actgcatgaa gatttgcttc
     6841 gcggtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt tttgtactct
     6901 tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa ggcgaataat
     6961 gccaccagaa tggcgcagaa agcaggcata tagagcgccg catgccagtc attgaaccag
     7021 gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg cgcacagttc
     7081 cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat agtacgacca
     7141 cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac aaacatcacc
     7201 gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc cgccgccaga
     7261 atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga acccatgatg
     7321 aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa atcaccgcgt
     7381 gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg aaccaaatag
     7441 taagccgcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg acgataagtc
     7501 ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa aatactcaac
     7561 attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt cagtgagaga
     7621 acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta aatgtgaatt
     7681 gccgcacaca ttattaaata agatttacaa aatgttcaaa atgacgcatg aaatcacgtt
     7741 tcactttcga attatgatcg aatatgcgcg aaatcaaaca attcatgttt ttactatggc
     7801 taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact cgcaatcaag
     7861 tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg actgtgccct
     7921 gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg ccaccgggcg
     7981 taaccacggc ctgctgcaca gcggtgcgcg ctatgcagta accgatgcgg aatcggcccg
     8041 cgaatgcatt agtgaaaacc agatcctgaa acgcattgca catcactgcg ttgaaccaac
     8101 caacggcctg tttatcaccc tgccggaaga tgacctctca ttccaggcca cttttattcg
     8161 cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag cgcgcattat
     8221 cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa gttccggatg gcaccgttga
     8281 tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg ccgttatcct
     8341 taccgctcat gaagtcacgg ggctgattcg tgaaggcgcg acggtgtgcg gtgttcgtgt
     8401 acgtaaccat ctcaccggcg aaactcaggc ccttcatgca cctgtcgtgg ttaatgccgc
     8461 tgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca tgttcccggc
     8521 gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca accgctgccg
     8581 taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga ttggtaccac
     8641 ctctttacgt attgattaca acgagattga cgataatcga gtgacggcag aagaggttga
     8701 tattctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgaaaacgc gcattttgcg
     8761 ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac gacccgagcg gacgtaacgt
     8821 cagccgtggc atcgtgttgc tcgaccatgc tgaacgcgat ggtctggacg gatttatcac
     8881 catcaccggt ggcaaactga tgacctatcg gctgatggct gaatgggcta ccgacgcggt
     8941 atgccgcaaa ctgggcaaca cgcgcccctg tacgactgcc gatctggcac tgcctggttc
     9001 acaagaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc cgctgcgcgg
     9061 ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg ctgagcgaag gccgtctaca
     9121 ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt gaagtgcagt acgcggtaga
     9181 aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg ggatgggcac
     9241 ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacggt ttaacgtcac
     9301 gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct ggaaaggcgt
     9361 gcaacccatc gcctggggag acgcactgcg cgaaagcgaa tttacccgct gggtttatca
     9421 gggactgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc attatgggcg
     9481 gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg cgctgtgcca
     9541 ttgtcacgcg tggtcaaagc gcgttgcatt tctcatccgg ttcgctggat ttgctgagcc
     9601 atctgccaga tggtcaaccc gtggcagaca ttcacagtgg actggaatct ttgcgtcagc
     9661 aggcacctgc acatccttac tctcttctcg ggccacaacg cgtgctcgat ctcgcttgcc
     9721 aggcgcaggc attaatcgct gaaagcggtg cgcaattgca gggcagcgta gaactggctc
     9781 accagcggat tacgccgctc ggcactttgc gtgcaacctg gttaagttcg ccagaagttc
     9841 ccgtctggcc gctacccgcg aagaagatat gtgtagtggg gataagcggg ctgatggatt
     9901 tccaggcgca tcttgcggcg gcttcgttgc gcgaactgga tctttcggtt gaaaccgcag
     9961 aaatagagct gccggaactg gatgtcctgc gcaataacgc caccgaattt cgcgcggtga
    10021 atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat gcgcttattc
    10081 ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggccta gccgatgaca
    10141 aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg ccaacgctgc
    10201 cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt gtgcgccagg
    10261 gtggagtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat ggcgtagtga
    10321 atgaaatctg gacccgcaat cacgccgata ttccgctacg tccacgtttc gcggttctcg
    10381 ccagcggcag tttctttagt ggcggactgg tagcggaacg taacggcatt cgcgagccga
    10441 ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag ggcgattttt
    10501 ttgcgccgca accgtggcag cagttcggtg taaccactga tgagacgcta cgcccgtcgc
    10561 aggcagggca aaccattgag aacctgtttg ccatcggttc ggtgctgggc ggatttgatc
    10621 ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat gccgctcaac
    10681 agattgccca acgcgcagga ggccaacaat gaacgacacc agcttcgaaa actgcattaa
    10741 gtgcaccgtc tgcaccaccg cctgcccggt gagccgggtg aatcccggtt atccagggcc
    10801 aaaacaagcc gggccggatg gcgagcgtct gcgtttgaaa gatggcgcac tgtatgacga
    10861 ggcgctgaaa tattgcatca actgcaaacg ttgtgaagtc gcctgcccgt ccgatgtgaa
    10921 gattggcgat attatccagc gcgcgcgggc gaaatatgac accacgcgcc cgtcgctgcg
    10981 taattttgtg ttgagtcata ccgacctgat gggtagcgtt tccacgccgt tcgcaccaat
    11041 cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg cttgatgcgg cgttaaaaat
    11101 cgatcatcgt cgcacgctac cgaaatactc cttcggcacg ttccgtcgct ggtatcgcag
    11161 catagcggct cagcaagcac aatataaaga ccaggtcgct ttctttcacg gctgcttcgt
    11221 taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg caatgggtac
    11281 cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta ccgctaatcg ccaacggctt
    11341 taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg aagctgtggg
    11401 agtaaaaggc attccggtga ttgccacctc ctcaacctgt acatttgccc tgcgcgacga
    11461 atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg aactggcaac
    11521 ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac cgctgccgct
    11581 gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc tctacaccct
    11641 ggagctgttg cgtaacatcc cggggcttga gttaacggtg ctggattccc agtgctgcgg
    11701 tattgcgggt acttacggtt ttaaaaaaga gaactacccc acctcacaag ccatcggcgc
    11761 accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg actgcgaaac
    11821 ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga ttacgctact
    11881 ggcccaggcg ctggcttaaa ctcccttctg atgcccggta agcatgtggt taccgggcat
    11941 ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa attcctctga
    12001 aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac cgtaatgtcg
    12061 cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct cctccccgca
    12121 tgatgcggta tttaaaacct ttatattcac acccgaaacc gcacgggatt ttctcgaaat
    12181 acatttacca gaaccactgc gcaagctttg caacctgcaa accttacgcc tggaacccac
    12241 tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt ccgtggaaac
    12301 cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg cagaaaagaa
    12361 tatggctttt cggccaatgc gctatgccac tgccgccatg cagcgtcacc tagataaagg
    12421 ctatgacaga gttccgctgg tggtgccatt gctgttttat catggcgaaa catcgcccta
    12481 cccgtactca cttaactggc tggatgagtc tgacgatccg caacttgccc ggcagttgta
    12541 caccgaagct tttccgttgg tggatatcac catcgtacct gacgatgaga tcatgcaaca
    12601 tcggcgtata gctctgctgg aactgattca aaagcatatt cgcgaccacg atttaatcgg
    12661 catggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca gccagctaca
    12721 aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc gttttattca
    12781 ggagattgcc gaacgttcac cactacaaaa ggagagatta atgactattg ctgaacggct
    12841 acggcaggaa gggcatcaaa ttggctggca ggaaggtatg catgaacaag ccattaaaat
    12901 tgccttgcgc atgctggagc agggctttga acgtgagatt gtgctggcgg caacccaact
    12961 cactgatgct gatattccga actgttatta aaaggagagg gagagattga tgactattgc
    13021 tgaacggcta cggcaggaag gacatcaaat tggctggcaa gaaggtaaat tagtaggttt
    13081 gcagcaaggt aaattagaag gtttgcagga aggtatgcat gaacaagcca ttaaaattgc
    13141 tttgcgcatg ctggaacagg gcattgatcg tgaccaagtg ctcgcggcca cccagctaag
    13201 cgaagccgat ctggcagcga ataaccacta attaacacag gcccacagcc gatccccatg
    13261 ggcctttgat atcaataact accttttatg cgtggcccat ttttgcctga tttaaacatc
    13321 gccagtcgtt gatccagggc atcgctgtag agcatcgtgt caacgccaac agcgacaaag
    13381 ttcgctcccc acgccaggca ttgctgcgcc atatcaggag ccacagccag aaaaccagcc
    13441 gctttacccg cagcacggat ccgccgaatg ctggtttcaa taattcgctg cacttccggg
    13501 tgcccggcgt tatccgggta gcccaacgac gcagaaagat ccgctggtcc aataaacacg
    13561 ccatcaatcc cttcgacgtc gaggatctcg tccaggttat ccagtgccgt tttactttcc
    13621 acctgcacta acagacaaag cgaatcgttg acttgcgcca tgtaattctc aatgcgtccc
    13681 cagcgcgcag cccgtgccac actggcccca acaccacgct caccgtaggg agggttggac
    13741 aaattttgcc ggagggggaa tgtgccggtg cagtaaaccg gcacagagtg gcgaaatcag
    13801 acggataaag tcatggactc aacaacctca atccagccat gctcactagc gatgtcctgg
    13861 ccgtttaacc aacggcgtag catattcagt gccatcattg cgcacacttc ctgacggata
    13921 gccaggctgt agcgtgtagt gctgaaacgc acacgcagag cgaaagtgcc gtctggcgtg
    13981 gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa cagccagtgc cagcccggca
    14041 aaatggttgg cccgccgttc tgtaatccag tgcgcagttt gcgccagggt ttcttcctgt
    14101 gaaggaatca cttcacacgc cagcaatgga gcacctgcgc gagaaagttg caaagccaat
    14161 aaaccaccgg tgaattgctc gctcaacgtc aggctgaact ggcggttttg caattcgcga
    14221 ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt
    14281 ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc
    14341 agttcgatga tcggcattga ggagcgatag cccattgtta cgcccggcgg cagttgtaga
    14401 gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa aggtagttaa acgcagacaa
    14461 accggcggct gtggtaaaga aaaacgctcg cgcaggcgcg gcaggatttc gtgctcgacc
    14521 atcaccttaa attctgacgg tacgccggga gtaaagaaca tcaggcaacg attaagctgc
    14581 acggcaaaac cacaggcggt gcctaccggg ttattgataa attcagcact ggcaggcagc
    14641 tccgcttgtt tacggctgct cggtgccatt actcgtccac gttcgtggaa atagcgttcc
    14701 atctctttga gccaggcttc atgcagcaca aggccttcac cttttgctgt cgcagcggcg
    14761 agtgcgctta aatcatcgct ggtcggtccc agcccgccgt taacgatcag cacatcggcg
    14821 tgctgactac gttcgcgaag aatggtgact aagtcatcaa ggttatcccc caccgtattg
    14881 cggcgagata atggcagccc ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg
    14941 tcaacgattt gcccgtataa cacttcatcc ccggtggata gcatttccac ttttaacatg
    15001 gttatctcct gcttttgtta tcgaatcact ataacgcaaa gggagataag gcgtagtaag
    15061 aaactgaatg ccgcgccgga agggatcaga aactggcgtt aaccccaaca tacaggccgt
    15121 cagccacagc gttgtcgcgg ttaccgtctt tacccgacag attcaggtag cgataacccg
    15181 cctcaatact gactggacgc ataatggtgt aacgcgcgcc agcattcgct tcctcataac
    15241 tttgaatacc gctggagagc gaatccggag agtagtaata ctcgccaaac aaacggaagc
    15301 tgttgccaat tttccactgc aaaccacctc ctaccgctgc ggcataacct tcatcgccgt
    15361 aattcgggtt ggtgtacacg ccttttccgc caacggtcgc cattaacggc ccgagaggca
    15421 gattcaaccc cagccccacg cccgcgacgt caccgtcgtc gtcgttatgt gtccagttac
    15481 cgcttaaagc caggcccgtc gattcagtcc caaaaccgac accaatattg gtgtattctt
    15541 tacccgcctg gccgctgatg ctcattgcat tgaccgatgc agaaaccaac agcattccgg
    15601 ccagacctgc tagcgcaatt tttttcatta tcccctttcc cacaagcaat taatcaaaat
    15661 tcccaaagct gcgggattgt actgcctggc acagaggatt caatgagcga aaaaagggat
    15721 ctagtcactt attgtaacta aatacttctt aaataattaa acgccgaaaa aataaacaca
    15781 ctttaccgat acacacaaaa ataataataa gtgcagttat cattgcaaaa gagattactg
    15841 tcactttcac atatttctga gttaccgtat tctcatggtt tctttttatt tataaggaat
    15901 acccctgtgc gacaacggac tattgtatgc cctttgattc aaaatgatgg tgcttatttg
    15961 ctgtgcaaaa tggccgacga tcgcggcgtt ttccccggtc aatgggcgct ttcgggtggc
    16021 ggcgtggagt ctggcgaacg aattgaagag gcactacgcc gcgaaattcg cgaagaactg
    16081 ggagaacagc tgcttttgac agaaatcacg ccgtggacct tcagcgatga tattcgcacc
    16141 aagacatatg cagatggtcg caaggaagag atttatatga tttacctgat ttttgactgc
    16201 gtttctgcca accgagaagt gaaaataaac gaagagtttc aggactacgc gtgggtaaaa
    16261 cctgaagatc tggtgcatta tgatttaaat gttgccaccc gaaaaacgtt acgtttgaaa
    16321 ggtcttcttt aacaatgatg acagccatca ctggctgtca ggttgaaaat ttagtggtta
    16381 acgaattccc catccagata aactttgcct ttttccacat gcatgactaa accatccaga
    16441 taatcaggtt taaatatcgc atcacgctta tttttagcaa tatatgtcag gcaatgatta
    16501 tgggtgaaaa taaccatatt tttatcaggc gctttgcttt gtaagtcctt aattgcacta
    16561 taaatctcat taccgcactg aagaagtcgt ttatctaccg tcagcttttt acccgcggaa
    16621 aaccaggtcg ccgactgaat ggtccggacg gtattactgg aataaagatc gaaatcaggg
    16681 atgtcagcgc taaaagcgtt gcccagttca cgggcatcct gggtaccttt aaccgtaata
    16741 cctgttttat ctgacaagca ttgattggtt gaacgatcgc aacgttcagc atggcgaaac
    16801 aaaacgacaa ccgggtgctg ctgtgccagt ctggccaatg ttttattgtc gatacgtggc
    16861 aaaccattgc tactccaggc ggcatgagta cccagtccgg caattgcagc gagcgccagt
    16921 aaaatgatga aatatttttt tgacttcaat gatgagcggc aaaaagctaa cacgtaaact
    16981 ccacctgtag acaagcgcaa ccagacaatt accgtgaaat tgagcgacat ttctggcgat
    17041 aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg gcaaaatgcc tcaaattttt
    17101 gccaaatgca aagcctaaat aagaaaaaat ataaaaattt caatatttac gtctaatatt
    17161 agtttcttaa ggttaagtta atattctatc cttaaaattt cgctccaaat ggcaaaatat
    17221 acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga aggaaaagca
    17281 atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt
    17341 aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgagcaa
    17401 gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga
    17461 atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc
    17521 ctgacctggg tttcaaccct caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg
    17581 gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgaatcagc cattacgcca
    17641 cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacaccatt
    17701 cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt
    17761 acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc atttcatgcc
    17821 attaaaaata ttacctgtgc tgaaggtggc ctgattgtaa ctgataatga aaaccttgcc
    17881 cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacaaacc
    17941 tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa tctgaccgat
    18001 attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg
    18061 cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc
    18121 cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa
    18181 cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc
    18241 gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg
    18301 tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct gttcccggat
    18361 atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc aggacaataa
    18421 gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag
    18481 caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc gttggggaaa
    18541 gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc
    18601 gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg caattacggg
    18661 caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat tattaccctt
    18721 gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa agccgatgaa
    18781 ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaaca
    18841 gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat gggtgactac
    18901 ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa
    18961 cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat tgaaattcca
    19021 gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg cctgattaat
    19081 ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct gagtctgctc
    19141 ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat tttacgcctg
    19201 accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact
    19261 tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg caggatctac
    19321 accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag
    19381 gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg
    19441 aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc gataatcccg
    19501 gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagagac attccggttt
    19561 atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa ctgtcaccag
    19621 aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttt cagctcgctc
    19681 ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc
    19741 tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa
    19801 aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata
    19861 tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat
    19921 tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct
    19981 gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg gcatccgtac
    20041 tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca
    20101 atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg
    20161 ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg
    20221 tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagatgctgg
    20281 gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc
    20341 gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc
    20401 gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc
    20461 tgaatcatcc acattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg
    20521 agtatcacgt caaaaaatgt gatttcgtct tgccgctggt ggcgatagcc acgccgattg
    20581 aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta
    20641 tccgctactg cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg
    20701 ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga
    20761 ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtg atctgggcct
    20821 atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg atgggaccac
    20881 gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc
    20941 tcaatctggt agaaggttca ccaattaagc taattgatgg cggaaaacaa aaacgctgct
    21001 ttactgatat ccgggacggt atcgaggcgt tgtaccgcat tatcgaaaat gcgggaaatc
    21061 gctgcgacgg cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac
    21121 tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat ttcccaccgt
    21181 ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat caggacgtag
    21241 agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag ccgaaaattg
    21301 atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg
    21361 ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc gtggcacccg
    21421 tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt
    21481 tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt
    21541 tttgtggaag atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc
    21601 aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc
    21661 ggctaaacat cacgaagtcg gtctgcacgc ctgggatcac catgcctggc aagcccgtag
    21721 cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga
    21781 agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa
    21841 ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat
    21901 gccgttccgt ccactgctcg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt
    21961 acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt
    22021 actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga
    22081 aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt
    22141 gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg
    22201 cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg
    22261 atgaaatcgg tacgttacct tatcggcatc ttcgcgttta ttgcctgcta ttacctgtta
    22321 ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga
    22381 gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa
    22441 aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac
    22501 tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg
    22561 tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc
    22621 tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc
    22681 tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc
    22741 aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt
    22801 tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc
    22861 tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc
    22921 ccctggggac tggcgatagc gcagcgggag cctgacttgt ggcactattt tttctgggtt
    22981 gagcatattc aacgctttgc actagatgat gcccaacata gagctccgtt ctggtactac
    23041 gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac
    23101 acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg
    23161 ccgctgctgt ttttctccgt cgctaaaggc aaattgccca cctatattct ttcttgcttt
    23221 gcacctctgg caatgctgtt ggcgcattac gctttgctgg cagcaaaaaa taatcctctg
    23281 gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca
    23341 tttgtgatct ccccctgggg accaatgaac acgccggtgt ggcaaacctt cgagagctat
    23401 aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta
    23461 acaaacgtcg aaaagacctg gtctttcgcc gcgctttgcc cgctggggct ggcgttgctg
    23521 gtaggatttt caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag
    23581 atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc
    23641 gcaggtctgg catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag
    23701 ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc
    23761 gcaaactggc ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac
    23821 cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcgt tcgtcaggag
    23881 cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct
    23941 tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca
    24001 aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca
    24061 tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa
    24121 gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcgc
    24181 cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc gggagtacgg
    24241 tgtaatgggc ctgatatggg gattattcag cgtcattatt gcctcagttg cgcaattaag
    24301 cctgggtttt gcggcaagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc
    24361 tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct
    24421 ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt
    24481 gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg
    24541 gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat
    24601 tttcctgccc acgacaaaac aacgttactg agttttccct gccgctgtac actgccgttc
    24661 ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg
    24721 attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa
    24781 gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac
    24841 atgtctgtta tcttgtttat tgcagcacga ttttttgtgc cagccattcc attgcattat
    24901 cctgtttgct aagagttttc acatcaattt acagattaac agataaactc tttatcaacg
    24961 gcaatggaat ggcagaaatg agaagtgaaa cggagggtta attattgctg acgttgcacc
    25021 cactctttta gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa
    25081 gtcagccagc gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga
    25141 tcaacggttt gctggtcata ctccaccacc gctaccggcc gatgaccaaa ctccttgtcg
    25201 gcaacggaaa cgataaacac ctgcaaaacc gcaggatgtg cagcaataac acgctcgact
    25261 tcttccggct gaataccctc accgccactg aagaacagat tgtctaaacg tccgacaatg
    25321 gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta
    25381 accagtgaaa ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc
    25441 cacacttcat tattaacgat tttcacttcc cgacccggca gcggcaaacc aacgtctgcc
    25501 aggacgtcgg cttctttcgc acataccgtg gaggcaaact cggtcagacc atagccgcaa
    25561 aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg
    25621 ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt
    25681 gttggcacca gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca
    25741 cgcaccgtca tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg
    25801 tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac
    25861 acaccttgcg cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg
    25921 gtagaacccg aggtcaacgt cattgagcac agacgcgtcg gctgccacgc agcggcatgt
    25981 acgccttcaa ccagctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc
    26041 ggcaccagcg caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc
    26101 agctgagggt tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc
    26161 agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc tgccctcaac cacaccctgc
    26221 gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc
    26281 tcgtcattaa gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc
    26341 cagtcagaga agatcataac aactgctcca gtacatcagc gtccactaac ggcagtgggc
    26401 tccccggcca gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa
    26461 tggtgtctgg cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact
    26521 caatggaaga actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc
    26581 gtactttttc cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt
    26641 cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg
    26701 tttcacgggc aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga
    26761 tgcggtggcg ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc
    26821 aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca
    26881 ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc
    26941 ccggcatatc ggcaagtttg aggatcagat cgtccggatc gccattacac agcggtgccg
    27001 cacggtagtt ggctgcttgc ggcaacgtat ctgtcagttc tgccaatgca cagcttacgc
    27061 caaaggccac ggaaggcatc tgcggtagct cgcaatcgcc tgccagccag ttatttaccc
    27121 aggcaagcag cacgctttgc gcctcttccc agttttcctg gctgaagccc ggcagtgggg
    27181 agatctcccc ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg
    27241 tttttaaccg cctgtcgcgc agaagcaccc ccgcgtccat ggggatctgc cagcggtata
    27301 cctgcgcgct acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg
    27361 gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt
    27421 gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg gcgttcagcg cagctttcag
    27481 gcagcgcagc gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt
    27541 ttccagatcc gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc
    27601 gtcgtactga cggcacagga accagatttc tcgcgctttt ttctgcccga cgatacgagc
    27661 catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa
    27721 gatggcatta tctgctgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc
    27781 gatggagtag ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg
    27841 gaagtccagc acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc
    27901 acgcactttc tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat
    27961 cacgccgata ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt
    28021 cagaggacgg aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc
    28081 ggtggatttt tcataacgaa tgtcctcgaa accttcggag cagtcgtgcc attcaaccgg
    28141 tgcgtaaggc attgcttcat caggataaat catattgtgt cctttagtca aaaacgcaag
    28201 atctgcgcca gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg
    28261 cgaggaatga catggcagtc ggcagccagt tccgccgcta gggcgcggaa tttgctgtca
    28321 cgttcaccac ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca
    28381 ggctggacgg cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg
    28441 ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt
    28501 tgataccagt cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg
    28561 cgatcggaac gctgacgttc cgcacgttgt tcagcatttt gcagccccgg atgcccgcct
    28621 tcgataataa ccccacaaag ccccgccagc ccctggcaag ccgccatcat cgccacccgt
    28681 ccgccaagcg agtaccccac cagccagaag tcaaggatgt tgtaactaac caaggtttta
    28741 cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccatga
    28801 cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat
    28861 tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggttttccg
    28921 tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg
    28981 tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac cgtggtggtt ggcgtgcgcc
    29041 aggcgtcggc aaatgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct
    29101 cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcac
    29161 gctcgctttg cggcgttggc aacagcgaga aaatttgccc gccgttgttg ttcaccacaa
    29221 ttaataccag cggcgcagaa acctgacgca ataacgccag cgcgttgaga tcgtaaagtg
    29281 cggagagatc gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgcctgcgg
    29341 cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt gctgtacacc gggtaacctg
    29401 ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttacca acaaacaatt
    29461 gcccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat
    29521 cacggcgggc aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc
    29581 agggctggcg tttttctgcc ggatgcagct ccagccagtc ggcaatattg ccaattaagc
    29641 gacgtccgcg atggtgtgcc ggatcaagtc gcccttcaat gtcatcaaca atccagtact
    29701 cttctggttc acagcttgcc tgccattgca ggagccgttt ccccgtcagg ctgcttccca
    29761 gttgcaccac aatttgtgcc tgctgcagct cgctggtcgc tttggcattg cctaaccaaa
    29821 gatcggcaca cggcagcggc tgcccggttt gtgacagcac atcgccaatc agcggccagc
    29881 caagagtttg cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa
    29941 ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac
    30001 gaggcgcttc acgcagccac ggtttgtcgt cctgccacca gtcacccaga cgctgttgcc
    30061 agctaagccc ggtatcgtcc atttcgccat acagcggttc agcaaacggg cagttgatat
    30121 ggactccccc cgcatgaagc gtaccgagag cgtggtcgat ggtagaaacc agccaacgtg
    30181 cggggatatc ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca
    30241 ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg
    30301 cggttaagag aatcagtttt tctccggtta acccggcttc aatcagtgcc ggatagagat
    30361 ttgccaccgc cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca
    30421 gccccagcgc cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg
    30481 aattctccgc cgccgctaac gttaacggtg tagaacgcga gcctggggcg atacagatgt
    30541 gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg
    30601 cgcttactga catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa
    30661 aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa
    30721 gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca
    30781 cggacaatgc ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc
    30841 agggaaacgc agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat
    30901 tctcgggtga acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca
    30961 gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt
    31021 gaagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt
    31081 aaaacatcca gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc
    31141 atgttctcgc gctggttttt atcatccgcc atcagccact ctcctaactg ctgcgcctgc
    31201 ttatcatcag gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca
    31261 cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc
    31321 atgtaaaaat ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg
    31381 ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca
    31441 ccttcggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc
    31501 cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt
    31561 gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa gggagctttc gctgaatagc
    31621 gtcaaccgca gcgtggcttt accgccacag cgtcgccatt ccaggcgggg taaaagtaaa
    31681 ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc gtaagtcggc gtgttccggg
    31741 tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg
    31801 acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga
    31861 ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc
    31921 cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc
    31981 gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact
    32041 acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct
    32101 agtaccagcc cgacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag
    32161 tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta
    32221 acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc
    32281 tgatcggcgg gctcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc
    32341 aggtcgtcat cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt
    32401 aaaacctgtc actaactata gacaagagtt gttgattacg cctgaattac ctcgcgtgcc
    32461 atcccaatgt gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa
    32521 ctctggtaga agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag
    32581 tgatgcgtac atgtttcgag tgttttactc atcagctgct gccccacttt ttcaccacgc
    32641 aacgcttcac tgacaatcac ccgacctata acgacaggct caagatcatc atcacttttc
    32701 agaatcctcg catacgccac cagttcatca tttttccaac cgagaatatg gcgattatcc
    32761 cccgtcaaat catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc
    32821 gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga
    32881 tcttgccatt caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat
    32941 tttttgaaac gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca
    33001 acccgcacgc gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga
    33061 ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac
    33121 cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta
    33181 cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt
    33241 ccgcaaggta tccgtgaatt tgtggaaacc gcgcagcgga ttagcggctc atggaccgat
    33301 tatccgctgg aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa
    33361 gtaaccgctt atccgctgga acacccactg gaatgttatg gctatcgtat tgaagaacat
    33421 gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca
    33481 ctgtttcagg aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac
    33541 ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc
    33601 ggcccctgcg atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg
    33661 acgctggata taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag
    33721 gctgcgacac tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg
    33781 cgctatgatg acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg
    33841 actgaactgg cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca
    33901 gggtgaaact tgccctgtta tagcaacccg cgctacatat atccacccac acaggcatat
    33961 tgagcgattt ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc
    34021 tcttttcatc ttgattcagc acaacctttg caactaaaag gagcccatga tggttacagt
    34081 tgtcagcaat tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt
    34141 tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga
    34201 aagtattgag ttactgcata atattgcgtt aggttatgat gaatttggaa aaaaagctga
    34261 agatattctt taccatattg ttagaaaccc aacaaatgag accctatcga ttatccgact
    34321 tattaaaaat gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct
    34381 taaaccaact ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc
    34441 gaaattaatg acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc
    34501 aaaggtgctt ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa
    34561 aaatcgtcaa ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc
    34621 aaaaaatact caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca
    34681 cctggaagac gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca
    34741 gttattcccc ataaatactg gcggacactg gatattagtt tcgctacaga aaatagtaaa
    34801 taaaaaaaat aataaactac aaataaaatg cgtcatattc aactcattgc gtgcactagg
    34861 ccatgataaa gaaaattcac ttaagcgtgt cattaatagt tttaattctg aactcatggg
    34921 agaaatgtcg aataataata taaaagttca tttaaatgaa ccagagataa tatttttaca
    34981 tgccgatctt cagcaatact taagccaaag ttgcggtgcg tttgtgtgca tggcagccca
    35041 ggaagtgatt gaacaaaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa
    35101 ctatgctgac agatttaaaa aatattcagc agaagagcag tacgaaattg attttcaaca
    35161 tcgactggta aacagaaatt gttatttaga taaatatggc gatgcacgaa tcaatgcttc
    35221 ttatacacaa ttagaaataa aacactcaca acccaaaaat agagcatccg gcaaaagagt
    35281 gagttaacat cattgttaat caattcgacc agtcagtaaa tatgctgact ggtcgaacat
    35341 aatttgctaa aaagtcatca ctgactgatg tcagtcccac catctgccag ttcaatctac
    35401 gggtatttcc agcgtatcgc ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt
    35461 tgctgacttt tcttaccctc agatttctgc cccacttgtc aattcaatct gccaataaat
    35521 aacataatta tatttttatt cctcacagaa attccttttt aaaaatagcg aacccactgt
    35581 aaaaaacact ttttgtatac ctctaccaag cgtaataaaa taaacgctca taattcatta
    35641 taaataataa tgaaataacg ccttttattg ataatttaca tatgaaaaaa tttttccttt
    35701 tgttatatta tttctgtttt cactccctct gattattttt accaaccctg ggtcactgca
    35761 ttatcaccaa cgccccgaca cgtaagtcct gagcaattag aaaaaacagt tcgttatctt
    35821 acacaaactg tgcatccacg taatgccggc aatattgata atctgaatag gtccgcagag
    35881 tatataaaag agttctttgt cagtagcggt gccagagtta ccgcgcaggg cgtccccgat
    35941 tggctattac gactctgcgc ctggaagcca gaattaccct tacccggcaa tgtcctggct
    36001 ttatcccgat cagggagact ttattgctgt ggtcggcaga atgcaggata tcaacgccgt
    36061 tcgtcaggta aaagcggcat tgttgtcatc ccgggattta tctgtttatt ctatgaatgc
    36121 cccagggttt attcccggta ttgatttttc tgatcacctt aattattggc aacacgatat
    36181 tcccgccgta atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg
    36241 tgataccgca gacagattga attatcagaa aatggctcag gtggtagatg gtgttataac
    36301 cttgttatac aacagtaaat aataaaaatt tctcgaagcg ggtccagaaa cagcgacatg
    36361 tacattttat tttccctcgt ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa
    36421 ataaatggga atgattggct attttgcaga aattgactca gaaaagatta accaactgct
    36481 tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag gattacgccg
    36541 actggatatt gataaacgct gggatttttt acactttggg ctgacaggca cctctgcttt
    36601 tgatcccgcg aagaatgatc cgctgagtcg tgccgtgctt gatgaacaca gcctcgaaga
    36661 tggcatagat ggcttcctgg gattgacctg gaatcaggaa ttagccgcga caatcgaccg
    36721 actggaatca ctggatcgaa gtgagctgcg taaacagttt tccattaagc gactcaatga
    36781 aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcctc
    36841 gatcatgctt gatatggaaa agctgattag cgcgtatcga cgtatgctgc gacaagggaa
    36901 tcatgcgctg acggttattg ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac
    36961 atgtcggcgg ctttctgact tacaaactaa cagtttacat cagcggcatt gctaaccgca
    37021 caatactaat cagcggttgt ggccatacac ccagcaccag taccaacagt gcagaaatca
    37081 gcaccacgat accgcccgcg ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt
    37141 ccggtgcgtg caggtacagg ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg
    37201 aaccgacaac cacggcaccc accagccacc acaagtgtgc ctggacaccg accgccagca
    37261 cgtagaactt accgataaag cccagcgtca tcgggatacc ggccagagac agcatcatca
    37321 ccgtcatcac tgccgcgagg atcggacgat gccagaacag accgcggtaa gagaacaggg
    37381 aatcagcatc cgggccacga tacgggctgg acatcaggct gaccacgccg aacgcgccga
    37441 ggctgctgaa cagataaccg gccaggtaaa cccctaccgc ttccatcgac atctcgccgg
    37501 tttgcagcgc aatcagcgcc accagcagat agccgaggtg agagatagat gagtaaccga
    37561 gcagacgttt gatgttggtc tggctcaacg ccatcaggtt accgaagatg atggaggcaa
    37621 aggcgataat cgccagtact acgcgaatcg cttcgctgtc acccaccggt gcgtagagga
    37681 acagacgcat caccacaccg aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa
    37741 ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag gtggaacggc accagagaga
    37801 gtttgaagcc gaggccaaca atcatcatgc cgaaacctgc cagcaacagc ggctcgttga
    37861 gcataccgtc gccgaggttt ttacccagcg cgacaaacga caggtcgcca gactgcgcat
    37921 acaccagcgc cataccgaac agcaggaaag aagacgctgc ggcagaaagg atggtgtatt
    37981 tgatactggc ttccagcgaa cgtttctggc ggaaagcgta accgaccagg ccaaacagcg
    38041 gcaaagagat cagttcaata ccgaggaaca gagacgccag atggtttgca ttcgccagca
    38101 ggatcccgcc cagcgcggca attaacacca acaggtagaa ctcatccttg ttgtcgttat
    38161 agccttcaag ccacgggtag tcgaaagtac aggtggcgag gctcgccaac aataccagcc
    38221 cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg
    38281 cctggccaac aaaccagagc gaaaccagcg ccgcgttaag cccaataacc gagagcgtag
    38341 cgttgaggaa atgattgcgt cgccacgcaa tggagagcat cacaaccacc accgtcaagc
    38401 cgacgatcag caacggtagc agtgcgatca ggttttgtgg agttattgtc atggcgattt
    38461 acggccttgt agtagtaacg gaattaacaa accactgctg gatattgcca atcgcggagt
    38521 gcgaggtatc cagaatcggc tgcggataga agcccagcag taccagcagc accaccagca
    38581 acaggatcat aaacagctca cgcagcgaca tccctggcag ttcctggctg gcaatctggc
    38641 ttttcgcttt accgaagtaa gcgcgatgca acatcgccag cgaataaaca gatgcaaaga
    38701 ccagcccaaa ggtagagata acggtaatca ccgggacaac ctggaagctg ccgaacagaa
    38761 tcataaattc gccgacgaag ttaccggtgc caggcatccc aagcgttgcc accgcaaaga
    38821 acagcgacag tgctggcagc catttcatct tgctccacag accgcccatc atgcgcatgt
    38881 cgcgggtatg gatacgttca taaagctgac cgcagagaat aaacagaccc gccgccgaca
    38941 agccgtgcgc aatcatctgg attaccgcgc cctggtaggc caactggctg ccggtgtaga
    39001 ttgcaatcag cacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat
    39061 cggtctgagc gaaggccatc cacgcaccgt agaagatgcc gataacaccc agccacatag
    39121 cgattggcgc gaactctgcc gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac
    39181 cgtaagcggc ggttttcagc aagatccccg cgaggtccac ggaaccggcg gtcggagcct
    39241 gggagtgcgc atccggcagc cagccatgca gcggaaccac cggcattttg actgcgaagg
    39301 cgatgaagaa gcccagcatc aacagatatt ccacaccact ggacattggc gtattcagca
    39361 gctcttcata gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa accagcgcca
    39421 ggatggcgat caacatcacc agaccactcg cctgggtgta aatgaagaac ttggttgcca
    39481 ccgtgatacg cgttttaccg tcagaggctt tatgccccca cagtgcgatc aggaagtaca
    39541 tcggcaccag catcatttcc cagaagaaga agaacaggaa catgtcgatg gcaaggaaca
    39601 cgccgataac gccgcccagg atccacatca ggttgaggtg gaagaagccc tgatattttt
    39661 cgatctcttt ccacgaacat agtaccgcca gcacaccgag cagaccggtc agcacgacca
    39721 tcagcagcga cagaccgtca atggcgagat gaatagagat accaaaacgc gggatccacg
    39781 gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc
    39841 cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc
    39901 agcgtggcac cttgacgcca aagcgttcgg tctgccagca caggaagccg ccaataaagg
    39961 gaattaatat tagccagggt agtaacatgg cgatctttat tccttgtaaa agtcccgtca
    40021 ggaccggatt ttcaacgaat tctcacgaca gaaaattctc ttcggactgg gggtagtgtc
    40081 ggatgcgccg cgaaggcagc ttatccgacc tacgttctta tatgcccagg ggcaatccca
    40141 caatccttaa ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc
    40201 atggatgcca cataccagcg cagataaccg ttctcgctta acagcagacc tttacctgca
    40261 aagcgggaaa gaaccgccgg gatgttcatc attgagttca gcggatcgcg tttcagcaac
    40321 caggcaatac ccaggaacgg cttgacgaac actttgtcgt acagccagtc aaagccccag
    40381 gcgttgtacc accaggtact cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc
    40441 agagtacgtt tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg
    40501 ccagaggtaa tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc
    40561 acgccctgca gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca
    40621 atcagcggca ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg
    40681 tggaagacga tgaaaatcat acggaaggtg tagagcgagg tcataaacgc accgaccaga
    40741 cctgccacca tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta
    40801 ctgaagaagc ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc caccaggaag
    40861 cagagataaa ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg
    40921 tgatggcagg ccagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg
    40981 tgagtcatca agtggaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg
    41041 tagccaatct ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc
    41101 gcggcaaaac cggccagcag cagcgtaacc gccccgacaa tacccaccag atgcagaact
    41161 tccggcgtca tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc
    41221 atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc
    41281 catgtctgca acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc
    41341 gcccacatca gcatgttatt gccgtcagca aagtgcgctg gtgccagttc caccatttcg
    41401 cggaagttca gggtgcccag ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg
    41461 tcacccacac gggtcacgac gaacgctttc attgctgctg cgccattctt cggatcggtg
    41521 taatagaaac cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc
    41581 agcagcaggt tatcggcgag aaccagaact accatgctgg cgatgaacag gttggtgtag
    41641 gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta catgtgaata
    41701 aggaaaccca caccagtgac caccgagagc atggtcagcg acaggccgtc cagcaccagg
    41761 ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtatgcc
    41821 tgctcgccgt tagcgaagaa atcaacgccg ataaaggcgg ttacctgcgc cgccaggccc
    41881 acagagccaa cgccgacgat cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc
    41941 agcaggacga agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca
    42001 tctcacttac tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg
    42061 caaggccgat actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac
    42121 cgtcggtctg accccagtag cttccggcga ccacaaaggc cagcgcggag gcgttaatca
    42181 tgatttccag accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc
    42241 caagaacgaa taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc
    42301 ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc
    42361 ttcacgaccg acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc
    42421 caccgccagt acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt
    42481 accgtcgata ccctgatcgt taacaccgag gatggcgtaa acaatcacca ccagcatgat
    42541 ggccgacaaa attgccgggc caatccacac ctgcggtttc agccactggc gttcctgttc
    42601 gatttctgaa ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc
    42661 acccgcgtag acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac
    42721 cccggaaatc gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg
    42781 ggtgatcact cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc
    42841 cattgcccct ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct
    42901 gcttcgccct tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatattcc
    42961 gggtatttgc ccggaccgga gatcagcaga tcctctttct cgtaaaccag atcctggcgc
    43021 ttgtattccc ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct
    43081 tcgcacagac cacagaaaat gcagcgtgag aagttaatgc ggaaaaattc cggataccag
    43141 cgaccgtctt tggtttctgc tttttgcagc gagatacagc cgaccgggca ggctaccgca
    43201 cagaggttac aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga
    43261 ccacgataac ggggcggcag atagaccggc tcttccgggt acattcgcgt ttcgcgtttg
    43321 gcgaacgcgt gcaggccgat catccagata ctacgaacct gggtgccgaa acctactaac
    43381 aattctttta aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg
    43441 ccgttaccag caagttgatc agcgtcagcg gcaggcagat tttccagccg aaggacatta
    43501 cctggtcata acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga
    43561 acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga
    43621 agaacagcgt caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga
    43681 acagaccgaa cttcataccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg
    43741 cttccggctg gtcaaacggg tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa
    43801 taaaaccaaa gaattgcggg atgacgttcc acacatgcgc ctggctgttg acgatgtcgg
    43861 tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag ggaaagcccg aggaacactt
    43921 cgtagctcag ggtctgcgca gaagcacgca tcgcacccag cagcgagtat ttgttgttac
    43981 ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc tgccatcatc aggaagaaca
    44041 aaatcccgat gttcaggtcg gcaaccaccc aacccggact gactggcaca attgcaaagg
    44101 ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga
    44161 atttcgggat ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc agctggagcg
    44221 aaccgcccca gccaacacgg ttaggtccat aacggttctg gaacagaccc agcaggcgac
    44281 gttcgccaaa gctcatgaat gccccacagg tgacaaccac cagcaggatc cccaccgctt
    44341 tgaggatggt cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc
    44401 cttgagatcc tcaagatgcg cgccagccag caccggagca atgccggaca tacccatcgg
    44461 caagcccacc tgccctgccg tcagtccttc ggcgatttca accggcagcg tgaccgtatt
    44521 gccatcgtaa ctaaaggaga cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc
    44581 tgggttgagt ttgatgtacg gctgcggcat acggctctgg aagaccggag cacgctgtga
    44641 caattcatcg ctgccaaaca ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg
    44701 ctggaagcgt gccggtacgc tggtgaagta atccagaccg ttttcgctgg tttcaaacag
    44761 acgcacgccc ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca
    44821 cgcctgcggg gagttccagc ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt
    44881 cggctggttg ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg
    44941 cggctcatga acgctgatat tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc
    45001 acgggccagt ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc
    45061 cgccagttcc gggattttcg ccacaacagc gtcaattaca tggtcgagct gcgtccagtc
    45121 cacttcacgg ctcagcaagg tgctgtgcag cgagtgcagc cagcgccagc tttccagcat
    45181 gacagttttg ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc
    45241 ttcattatta attaccgtac cgtcgctttc agcaaagctg gcagcggaaa gcaccagatg
    45301 ggcgttttcc atgatcgctg tgcgttgatg atcaaccacc atcaccagcg gcgctttagc
    45361 cagcgcagca ttcacgcggg tagcagaagc gtaacgatgc agatcgtttt ccaacaccac
    45421 caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg aaccgccacc
    45481 cataatgccc agccccatgc tgttgacgga acgggcaatc atggtgatac caacgtcagc
    45541 gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc
    45601 ggcgttcgtc ccggagataa tcaacggttt cttcgcacct gccagtgcct gcacgatgac
    45661 gtcgattttg ttttgcagct caggttcgat accgtcaacc gctggtgcgg agttatccag
    45721 cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca
    45781 cgccgcgata tcatccagac gagtgtcatc aacgttggta acaaacagcg gatgcttcgc
    45841 acgttgaccg atgttgagga ttgccgcaat ctgccagtca gccactttct gtgctgcagc
    45901 catttcgcgc gctttacctt tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt
    45961 ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc
    46021 cggagtataa atgccgcctt cacgcagcac tttcagcgcc agttgcagac gttcctgctc
    46081 accgtgagcg ataccggtgt agaagttttc ttcgcccacc agttcacgca gcgcaaagtt
    46141 gctttccacg ctggcacgcg gagaaccaat accgatcact ttcttcgact gacgcagaat
    46201 atctgccgcg ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg
    46261 ctgtactggc tgacgcggac gatccttcag gttgacgtaa ccgtaaccga aacgaccacg
    46321 gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc
    46381 gccgtaacgt tcaccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg
    46441 cgcaaactgc atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac
    46501 gccggtcggg caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc
    46561 cgggcgaccg aagtagacgt tgtcgtgcgc gccataaacg cccagatctg taccgtcagc
    46621 gtaatctttg tagtaacgca cacagcggta gcaggcgatg cagcggttca tttcgtgaga
    46681 gatgaatggc cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa
    46741 gctgtgtccg gtcatcacgg tcatatcctg aagatggcag ttaccgcctt cttcacatac
    46801 cggacagtcg tgcgggtggt tggtcattaa ccactcgacc acactttcac ggaactgttt
    46861 cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat
    46921 caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca
    46981 agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga
    47041 cagacaagct tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg
    47101 aattgtagcc attagcatgc ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat
    47161 tcggttacca gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc
    47221 tgaacggctg tttgattccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta
    47281 acggctccac tgcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac
    47341 acagttgctc aagtgtttcg atatcgcccg gctggccttc gccacgctcc agcgcacgca
    47401 gaattttcac gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac
    47461 gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat gttgatctca tggtcaaccg
    47521 ccatcgccag cgccgtaccc agacggctgc ccgctttacc gatactttcg aattccatcg
    47581 gcagatcaag gtgcgcttcg gtcaggaagt cagtccccgc gccgcctggc tgccaggctt
    47641 taaatttcag accatcacgc ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg
    47701 tgccgaacgg cagttcccac agtcccggat ttttcacccg accggagaag cccatcagct
    47761 tggtgccagc atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga
    47821 tcgccggaac gttacacagg gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc
    47881 cggaggttgc cgggaagggg ggtttcgagc gtgggttagc acgacgccct tccagggagt
    47941 tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt
    48001 cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg
    48061 caatagcgcg gcgcagattt actgccgctt cgatatattc gccacgcagg aagatgtagc
    48121 cacggtaagc tttcagcgca aacgcggaga tgagcatacc ttccaccagc aggtgcggca
    48181 gttgctccat caacaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca
    48241 gcaggtaacg gatgttcatg gattcgtctt tcggcatcag gctccacttc aggccagtcg
    48301 aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt
    48361 ccggagacag cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcggtatt
    48421 cgtccagcca cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg
    48481 gagtacggat aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt
    48541 cagatgcgcg tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag
    48601 gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt
    48661 cagctttttc tcgagcgccg cctgaatacc ctgatacccg ttgatatgac agaccacgct
    48721 gtcgcaataa cggatcacat ggcgaccaac cggctggcgg aagatctgac tgtagaacgt
    48781 tgccacacct tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc
    48841 accatccggc acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc
    48901 acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag
    48961 ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac
    49021 ataacaaaat cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc
    49081 gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc
    49141 atggtgctac cgtcgctggt caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc
    49201 tggaaagatt cattggcagg catcaccggc ccccacgaca cttgcaggaa gtgggtgatc
    49261 agggtttcga tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc
    49321 gctttgaacg ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg
    49381 cgcagctctt ccactttcag cattacgcgg gtgtagcagt cagaaacacc accaccaacc
    49441 gggatttcaa agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag
    49501 tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg
    49561 ccataggcgg caacgccctg ggtacgacct ttcagaatgg tattttgcag cgccgctttc
    49621 tcgtaagacg ccagacgttt cggcatccag tcgaggaact cacgcagcag acgatcccag
    49681 ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg catacggaaa
    49741 cccgtgatcg cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg
    49801 ggcgtcattg cgccgacgtc ctgaataaag gtcgagatgt acagcaggtg gctgttgata
    49861 cggaacagtt cggagagcat aacgcgaatg acgttaacgc gatccggcac ggtgatcccg
    49921 gccagtttct ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattca
    49981 atacggtcgg tatacggaat gtagctgtgc caggactggc gctcgcccat tttctccgca
    50041 ccacggtggt ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt
    50101 aaaacgatac ggaaagcccc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg
    50161 aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa ggtcagggct
    50221 tccatctcca gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc
    50281 gccgggtaat ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc
    50341 aggttcgggt gaccgtcgaa agtaatgcca aacagatccc aggtttcacg ctcataccag
    50401 ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc
    50461 accttcagca tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa
    50521 tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca
    50581 aacagcatga cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt
    50641 tcacgcttga tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc
    50701 ggcccaaaac ggttgcgcag ttcgccaatt accggatcat caagatgatc gcgggtctgc
    50761 caggcgggtt cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg
    50821 tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct
    50881 ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt
    50941 tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca
    51001 accacccagg agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc
    51061 tgcatgtacg cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat
    51121 ttatcgacgc cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggcc
    51181 cccattgaga taacccattt tggttccagc atctggtcat acagacgctg aataaccggt
    51241 gccattttgg taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggcgaagca
    51301 cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga agtcaccatc
    51361 tcaacgtaac agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag
    51421 ttaaccatgt cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg
    51481 tcggttacga tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg
    51541 cgggtgagcg tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg
    51601 ttcgtttccg ggttcataca ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca
    51661 atacgcacca gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca
    51721 aagcctaccc agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca
    51781 acgtcgaaga taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg
    51841 gcggagccga ccgaatcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg
    51901 cgaccgccta aaaaccaacc gcctaccagc atcaggcaac acaggccaat ggcaacgata
    51961 agaaagatag cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt
    52021 gcttactcat caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc
    52081 gattcatggg gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct
    52141 aattgatgtg gtcttttact cctttctata accttttgtc aactttaaca aaagtttctt
    52201 cacattagtt tacataatat caacaccatt agcatttaat gccctttcac cccagatcct
    52261 tgacgactcc aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa
    52321 atacgggtct attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc
    52381 ttgatctgtg acacgctttt gttattccat aacaaaaacg cagcaacaaa tttacgtatt
    52441 ttttaacatc attgtagcag gtgatttttt tcaggcgatt atttgtgcgt tcgggacgtg
    52501 aatctctggt gattgaaaaa taaacagttt tgtacgttct gcactatgcg aggaaggatt
    52561 tttactaaaa aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat
    52621 tgtctcagga attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa
    52681 tcatcccctt ccggagcaga cattgggctg tattgccacg gattatggta gctctccatc
    52741 gcctgataga tcacctgggc cagctcatta ttactggacg ggtcatagca cagcaaatat
    52801 tcggtatcag gtagcggcgg taagccatct actccactca gcacacgcag gtccgggctc
    52861 atcatctcaa ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc
    52921 ggaagcgtcg aggcgacata agccagtcgc catggaatat ctgctttatt aagcgtcgcc
    52981 agcaccatat cgcgaaacgg acttggatca tccagcaata caagagggat cggctcgcct
    53041 ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat
    53101 gccttaaacg ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc
    53161 atttctgcca tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc
    53221 gaactcacgc gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg
    53281 atagttaaca cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa
    53341 cgcaggattt tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg
    53401 cgaccgtgac gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga
    53461 cttacggcgg actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg
    53521 gcaacagcaa caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca
    53581 cttatcatat attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca
    53641 caaacaagca attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga
    53701 aagtaaaaat gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc
    53761 tgaagtagat cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt
    53821 tctatttatg acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt tccactctcg
    53881 tgcagcatcg ctggtcatac gcgaacacgt acaaacagca atggtgaagc ggcatcaaga
    53941 gcagggatcc gtcatttatg cgagcatttt accccaaaac cttttattta taagggtcat
    54001 tgcgaattat ctgatgcaaa gttatgttat gttatgttat gttatgttat gttatgttag
    54061 gcaaagtaat cttctattat taataagcac atcaaaacct ttttgaatat taaataataa
    54121 ttaattagca tcatcctcat tcattaattc cgcttaacaa tagtttcaca ccttatcccc
    54181 attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga aatatcatca
    54241 tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct tacagataaa
    54301 aaaaccagac ttgcaattaa gaattagaac aattcactat ataacattac atgtaaagca
    54361 tatacacctc attattttgt cattattaag ttattaacag cacaatcgag ccttcccctc
    54421 tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac attgttctaa
    54481 gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt catgtccccc
    54541 attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc ggtgctgaaa
    54601 gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat cggcaaccca
    54661 gccccattcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg caacctgcct
    54721 accgctcaag ggtattgcga ttccaaaggg ctttactccg cgcgtaaagc catcatgcag
    54781 cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat cggcaatggt
    54841 gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga cgaaatgttg
    54901 gttcctgcac cagattaccc actctggacc gcggcggttt cgctttccag cggtaaagcg
    54961 gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga tattcgcgct
    55021 aaaattacgc ctcgtacgcg tgggattgtt attatcaacc caaataaccc aaccggcgcg
    55081 gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca taatctcatt
    55141 atcttcgccg atgaaattta tgacaaaatt ctctacgacg atgctgagca tcactcaatt
    55201 gcgccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa aacgtaccgc
    55261 gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca cgccaaaggc
    55321 tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt tcctgcgcaa
    55381 cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat tacccctggc
    55441 ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat tccgggcgtt
    55501 tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc caaacgcttt
    55561 aacattcatg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa agttctgttg
    55621 gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt cacgctaccg
    55681 cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc tggttatcat
    55741 cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagtgttatc ccgccacaat
    55801 gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt tttgcccatc
    55861 tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgcgg acggaaaatg
    55921 tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct atcaaaaatc
    55981 ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg taccacgatg
    56041 cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat gcgcaaatcg
    56101 ctcaggagta caaggctatt gaaaaaatcg ctcagcagaa actggtcgat atggttccgg
    56161 aagaactgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc gatgaagaaa
    56221 aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg gaagaactcg
    56281 cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg cttgaagcgc
    56341 gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc catctttcgc
    56401 tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc gcatcaggca
    56461 ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg ggatcatcac
    56521 cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa agtcactgaa
    56581 gctgtaattt cccggaccta aaaccagggt gttaacaggt gaagacaccg gtgtcataaa
    56641 ggcggcggat gctgccatcg ccacgaccat cgcaaatgga taaggcgaca ctcccatcgt
    56701 tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat tagagataaa
    56761 cagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg gcccataacc
    56821 gccgccaata tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt tctgtaatgc
    56881 cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa tggatttata
    56941 ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga tgatagcggc
    57001 aacaggatta ggaatttcat ttgtcagcat taacgccacc attagcacca gacagaaaat
    57061 ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg gtaagttcag
    57121 cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt tacccacaac
    57181 caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca gcgccacgcc
    57241 attgcgcttg agccccacca cattcagtcc gtagcgggta cgaaaaccaa tttcgcgcac
    57301 cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc ccacatcaag
    57361 ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt gctcactaca
    57421 aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt cacgcgcgcg
    57481 aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc agcgttcaac
    57541 accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc gttgacctat
    57601 cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc gatattcacg
    57661 gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct gggtatcccc
    57721 tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca cgaccaggcc
    57781 aataggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca attcactgtt
    57841 gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca gcccggcaaa
    57901 acttaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa cgcttaacac
    57961 cacgggaata aagatagcga caacgctggt tgaactcata aacgcgccaa gccccgcgac
    58021 ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgccactt tgaccagcca
    58081 tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa taaacaaggc
    58141 ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga ccgtcagcgt
    58201 tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac gcactctgcc
    58261 cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca attcaccgtt
    58321 cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa aagccccgac
    58381 gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc atttggctgt
    58441 ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt caggcgcggg
    58501 gtatctgccg gagcgttaac cgcaatgaca tggcaacccg ccgctaggcc agaaagcacg
    58561 ccagcgggag catcttccac caccacacac tcctgcggcg caagccccag cagctgcgca
    58621 cctaacagat acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc ggttacaaac
    58681 acctctggtg cgggaagccc cgctatttta tggcgcgctc gcgctaccgg catggagcca
    58741 gaagtcacaa tggcccacgg aatacctgct ttattcaaat gattgagtaa ggcgattgcc
    58801 ccagaaagcg cggtaatacc ttcggtttcc gtggcctcga tttgctccag acgcgtaaac
    58861 tcggcggcaa tatcagcctc ggatttgccc gccataaaat ggcgcaaaga ggtgatcgcc
    58921 tgtttaccgt gaatgaaagc cagcacctct tccggcgcta acccatgacg tctggcccag
    58981 ttgctccacg cccgttctac cgcaggcagg gaatccacca gcgttccatc aagatcaaac
    59041 agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg ataatttgat taatttcgtt
    59101 ggcgctcaaa tggtactgac gcgggcaggc atgccacaca ttaagcatgc gctgatattt
    59161 ttcccacatt ggcgtctggg cgttaaaacc atgagtccca gcgtcaaaat gggtatagcg
    59221 cccttccaca ttaaccataa agcggacata accgaggtaa cgtgcttcag tggcggcgtc
    59281 aaagccgagg aaggtgacac gacgttcatc gatggattgc tggtcctgca aattagacca
    59341 ggaaacatgc aacgcatgat acatctccat aatgtcgatg atagtgcggc aggtttcttc
    59401 tttcagctcg ccaaactcgc gatccaattc acgcatctgt aatccgtaac cacgctcaat
    59461 aattgtttgc aggcgacggt aacgttcagc atttgccgga tcgagcatag tcatcatctt
    59521 gtactgatta gacaaaataa gacgttgcgc gttggtcatt tccattgttg actcctgtat
    59581 cactctacta cggtgaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag
    59641 gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg
    59701 tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact
    59761 cctgcaattt accgcgaact tcataaaacc agttaaggat tgcagggggt aatggcgtta
    59821 cagaacgctt gcccagccac cacaatccct gcatgggtaa acttaaggcg aacagtgcag
    59881 tggcaactgc cggcccaagc agaccgccca gggcaatctg ccagcagaga gtaaatacgg
    59941 cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa
    60001 agaccggggc aaggcgtttt tccagtggcc acgtctttga gtaatgctgt ccgcggcgaa
    60061 acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca
    60121 catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaacg ttcaaaacat
    60181 tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta aatagtacat
    60241 attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg
    60301 catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc
    60361 ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg
    60421 tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt
    60481 cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag
    60541 ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc aataaacagg
    60601 aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta
    60661 ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac
    60721 acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca
    60781 aagatgcagc ttcttttgca ccgctgcaca acccggctca cctgatcggt atcgaagaag
    60841 ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc
    60901 accagactat gccggaagag tcttacctct acgccctgcc gtacaacctg tacaaagagc
    60961 acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg
    61021 caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg
    61081 gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg ggtctgaccc
    61141 cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc
    61201 acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt
    61261 ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg
    61321 cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca
    61381 tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgtattc actggtggta
    61441 tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct
    61501 ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca
    61561 aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag
    61621 acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt
    61681 aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat
    61741 ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga
    61801 acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga
    61861 tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga
    61921 accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat
    61981 ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga
    62041 aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa
    62101 aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct
    62161 gaaagagcgt atcgaactga cccgcaacag cttcggtggt gccaaaaaca ccaacatcac
    62221 cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga
    62281 tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa
    62341 gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct ttgacctgat
    62401 cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga
    62461 catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct
    62521 ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt
    62581 ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg
    62641 ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc
    62701 ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacct
    62761 ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta
    62821 catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc
    62881 tccgccagcg ttccgttatc agctgactga actggcgcgc aaagcgggca aacgtatcgt
    62941 tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg
    63001 tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca
    63061 gggtgtagaa ctaggtgcag gcattgaaat cgttgatcca gaagtggttc gcgaaagcta
    63121 tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga
    63181 acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg
    63241 tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat
    63301 caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca
    63361 ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga
    63421 aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat
    63481 gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaaag ttcgcgaagc
    63541 aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga
    63601 cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg
    63661 cgctaccgtg ttcatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca
    63721 gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca aaccggttaa
    63781 cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat
    63841 tcagtctgca cagcagcagt aatctcgtca tcatccacag ctttgcgctg cggatatctg
    63901 aaccggaaat aatcactatt tccggttttt tattctctta atttgcatta atcctttctg
    63961 attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tctcactttg catacttacc
    64021 actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac
    64081 aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag
    64141 tcttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt
    64201 tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa
    64261 cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc
    64321 gggcctgatg aacttcccgt ttgaagggtt aacctcagga tcgaaatacg ggacagccgt
    64381 tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg
    64441 aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt
    64501 tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga
    64561 ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat
    64621 taccaccgtc ctggtgactt atattgccac gcaaatcggt tttgccagtt cgtggatgaa
    64681 cccgttttgt gtggttgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg
    64741 gttgcgcatc gtggtgtggg ttatcgccac tctgattggc ctgatcttta ccatggtgta
    64801 cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt
    64861 tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt
    64921 gattgtcctg acagccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt
    64981 tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt
    65041 tgttttccgc cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc
    65101 gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt
    65161 tggtaatggt gaagcaggta atgccagcgt gttaaatact atcctcaaca gcattgccaa
    65221 tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggctgt
    65281 atttaatttc ttcgtgacgt ccggttctgg tcaggcggca ttaactatgc cgttactggc
    65341 accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcgt tccagtttgg
    65401 tgatggcttc agccacatca tttacccaac ctcagcttcg ttaatggcaa cgctcggtgt
    65461 gtgccgggtg gacttccgta actggctgaa ggtgggcgcg accctgcttg gactgctgtt
    65521 tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta
    65581 agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt
    65641 ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca
    65701 gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag
    65761 ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc cagacacggc aatttttatc
    65821 ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc
    65881 cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tgaactacac cgcctgcggt
    65941 cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt
    66001 gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat
    66061 ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc
    66121 agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac
    66181 ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg
    66241 atcattgagt gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt
    66301 accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc
    66361 tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag
    66421 attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac
    66481 ctgttgccac ggcgcggtta tcgggaaatg cagcatcatt tgatccactt cgctgtcgca
    66541 gttgccacgc acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc
    66601 tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat
    66661 caccagccac tgggcaccgc tttggacaaa caactccaca acacgttccg tcgccggtaa
    66721 cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg
    66781 taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa
    66841 taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttctg
    66901 gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa
    66961 tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgttct gccatcgcga
    67021 acagtttctc tgcactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
    67081 agacaacatc cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
    67141 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
    67201 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
    67261 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
    67321 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca
    67381 acgccaccca ggcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga
    67441 tagcgggttt actcattatg tcctcggcaa taaagggggc tggctatcaa actatagagc
    67501 ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg
    67561 gtgttcagat gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
    67621 caatggtcac aaaattacgc tgtttctcga agaagcagag cttgattatc gcttgattaa
    67681 ggtagacctg gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa
    67741 caaaattccg gcaattgttg atcattctcc tgccgatggc ggcgaaccgt taagcctctt
    67801 tgagtctggt gccattttgt tgtatctggc tgagaaaaca ggactctttt tgagtcatga
    67861 aacgcgtgag cgcgccgcca cattacagtg gttattctgg caggtaggcg gactggggcc
    67921 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
    67981 tgaacgttat caggttgaaa ctcagcgtct ttaccatgta ctaaacaagc ggctggaaaa
    68041 ctcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
    68101 taatgcctgg actcgcctgc gaattgacct cgcaatgtat ccggcagtca agaactggca
    68161 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aactcggtga
    68221 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac
    68281 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgtttg
    68341 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
    68401 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
    68461 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
    68521 ctggaaaaat taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat
    68581 gctgaagtgg agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc
    68641 ttaagctggc agcgttaatc gccatatcgg gaggctgcat gaacatagtg atcaccggag
    68701 ggacgggatt aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatta
    68761 cggtagtgac gcgtaacccg cagaaagcca gttccgttct cggccctcgg gtgacactat
    68821 ggcaagggct tgccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg
    68881 gagaaccgat tgctgataaa cgctggactc acaagcaaaa agagcgtctc tgccaaagcc
    68941 actggaatat cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccgccgtcgg
    69001 tactcatttc cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg
    69061 aagaggaacc gccgcataac gaatttactc ataaactctg cgcccgctgg gaagagattg
    69121 cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgaaccggc gtggtactgg
    69181 caccggacgg cggtattctc ggtaaaatgc tgccaccgtt tcgtcttggc ctgggcgggc
    69241 cgattggttc cggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggca
    69301 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
    69361 tacgcaatga acaatttgcc catgcgctcg gtcatgcgct gcatcgcccg gctattttgc
    69421 gcgtccccac aaccgccatt cggctgttaa tgggcgaatc ttcagtactg gtattaggtg
    69481 gacaacgcgc gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt
    69541 tagaagaggc gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt
    69601 aactccctgt gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg
    69661 tcaccatcat aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca
    69721 gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca
    69781 tcagtttctc cttttcttgt ggtgcgcgtt ctgttatctc accaataaat gcacggaaac
    69841 gctgggcatc ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag
    69901 tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    69961 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    70021 ccggcaccac ggtaatatcc accaacggaa acgctgatga atatattttg cgggctatag
    70081 caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    70141 gataaaacag catcgggatc accaatggaa gctctttata gcccgcatca agatggtttt
    70201 gcattgccgc aatggaataa cgcatcatgc gaaaagccat taattcttcc ggcttacttt
    70261 ggtgctctat cactacataa atataaccca ctccctcctg cgttttcaca gaccacaaga
    70321 ggtcggaata atattgccgc aggtcttcat caataaaact gtttggttct agtttaagcg
    70381 tcgttaaatc acacagtttg cgcagcggcg cgggaagatg aatattaata aaattccgcg
    70441 cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg
    70501 atattgtcat ggcgttccgt cacccctcaa atttaagatg acgcgacaat aaccacgatt
    70561 aacacccaat gcagcaggca gtttttatct ttggacagcg aattcagaag tatttttagc
    70621 ggactgcaac ttttacattt cagcggaagc tgactcgcaa attccccttg aaaatactcc
    70681 ggcagcgtta gccaccggag aggaaattat ttcagcgatc ccttaaggaa ctgttgcaga
    70741 cgagggcttt gcgggttgcc aaataactgc tccggcgcgc cctcttcttc tattttcccc
    70801 tgatggagga aaatgacgtg agtagaaaca tggcgagcaa agcccatttc gtgagtcact
    70861 accaccatgg ttttcccctc ttctgccagt tgctgcataa tacgtaacac ttcgcctacc
    70921 agttcaggat cgagcgccga ggtaggttca tcaaacagta aaacttctgg ttccatcgcc
    70981 agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg aaagatgcac cggatatttc
    71041 ccctgcgcac gttcgtctat cccgactttc gccagatact tcaccgcccg ctcgcacgct
    71101 tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc caacaccgtc
    71161 atatggctcc agagattgaa gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc
    71221 agttgatttt tatcggcgac tttgagttga ccgtctttgt cgcgcaccag attgatcgtc
    71281 tggccattga ccacgatcga tccttcactc ggtttttcga ggaagttaat gcagcgcaga
    71341 aaggtacttt tccccgatcc cgacgatccg atgatgctta ttacatctcc ggcattcgct
    71401 tgcagtgaga cccctttcag cacttcgtgt tcgccatagc gtttgtgcaa atcgataacg
    71461 tttaatttat tctcggacat cgtattctca gtgcgttgaa gaaggtttca catgctgcaa
    71521 ccagcgtttt tccgctctgc gaaagaggct gatcaggaca taagagatga ttaaatagag
    71581 caccgcggca atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa tatcgcgggc
    71641 tattttcagc agatccggca cagtggccgt aaacgccagc gcagtagagt gcagcatcag
    71701 gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca aaataatgca
    71761 gcgatacatt ttaaaggtcg agaagccata ggctctggcg gcttcaattt ccccatgcgg
    71821 aaccgaacgg attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag
    71881 cgccagcacg gtacagttca ggccactgcg gaagaaagcg ttaaggaatt cggttccctt
    71941 aacaatctca agcgtgtaca tgccggaata gaacaccagc aactgaacat acagcggcgt
    72001 accgcgaaaa atataggtaa ataaccagat tggaaactgg atgtatttat tactggagac
    72061 acgaccaatc gccagaaaca gcgccaggac tccgcctatc actaccgaca aaataagcag
    72121 ccacagagtg atcgccacac cagtaaagcg ataaccgtcg gtccacagca gcggtttcca
    72181 gtattcatgt aagatttcga tcacaggtca gccctcttca cacccacgga gtagcggcgc
    72241 tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa ccaggtaaat cacgccacag
    72301 acgatggcga aatagaacgg ttcccaggta cttttgcctg ccagctgcgt ggctttgacc
    72361 acatcttcca ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac ctgccagttg
    72421 ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa acatgatcct ccgaaacact
    72481 tgcccgcgag taaaaccgaa cgccgtcgcc gcctctatat gccctttcgg cactgccata
    72541 aacgcgccac ggaacgtttc ggtaaaataa gcaccgtaga taaaaccgag agtgataata
    72601 ccagcgacca tcggatcgat atcaatctgc ccgacgccca tcgcctccgt caccatgttt
    72661 agcgcaatct gcaaaccata gaaaatcagc agcatcaaca ccaagtccgg cacgcctcga
    72721 atcagcgtgg tgtacccttc gaaaatcagc cccaaaagcc gattttgcga gagcttaccg
    72781 ccagcgccaa ttaaaccgat gattacagcg agcactacag agctgatagc cagctccagc
    72841 gtgacgagcg caccctgtaa aataacacct gaaaacccat acaacatgct gcctgtcctg
    72901 tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt acccgaaggg agcgggcttt
    72961 ctttcaccga tggcgattaa ccaccataaa catcaaaatc gaagtacttt ttcgctaatt
    73021 tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt gttcagtgcc tcgcgcagtt
    73081 cgttatcttc tttacgcagg cccatgccgg tccctacgcc aaacagtttt tcatctttaa
    73141 cagacggacc accgaatttg taatctttac cgacgggttg tttgaggaaa ccttcgctgg
    73201 cagcgacctc atcctggaac gcggcatcaa tacgtccggc agtcaggtca gaataaatgt
    73261 tgtcctgccc ctgatacgag acgatttcaa tgccttttgg tgcccagtgt tcattaccga
    73321 acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc gactcgactg
    73381 tcggctgaat gtcagaattt ttcgccacca ccagacgaga atcggcagcg tacagtttgt
    73441 cggtgaaggc aatttcttgc tgacgttttt ccgtaatgga aagcgatgac atgatggcgt
    73501 caatcttctt cgcttttaag gacgggatta acgcatccag cgggttttcg acaaacgtac
    73561 attgcgtatt gatgcgcttg cacaactcct tcgccagctc gatatcgaaa ccaaccagtt
    73621 cgccttgtga attttttgat tcaaatggcg cataggtcgg gtcggtaccg atgcggatgt
    73681 tttgcggaat tgcagcaagt gccgcagttg cgctggagaa ggccagaacc agagagagcg
    73741 atagcaccag ttttttcata atgtcctcaa ctgacagtct ttttataggg aatatttaca
    73801 ggtttgttac acttatcgtg ccataaaatt gaccatcaag gcaaaatatc ccgccccgtt
    73861 agacgtatta tttttcatta aaaatgctta agtatgcatt tcaatgcacc acgatagtgc
    73921 aacaaacctg gcgtgcacca tgatgatcat tccatcaggt acagcttccc agcgacgtat
    73981 cagtcaccgt agacattaaa gtcgaaatac tttttcgcca tcttgtcgta ggtgccgtcc
    74041 tgacgcagct cgccaagcgc cttattgaag gcagccgtca gttcagcatc atctttacgt
    74101 agccctacac cggtgccatc accgaagtat tttttgtctt ttactgatga gccagcaaag
    74161 gcgaaatctt taccagcagg ttgcttgagg aatccttcgc tggcagcaac ttcatcttgt
    74221 aacgcagcat ccagacgtcc tgcagccaga tcggaataga ccaaatcctg gttggcatag
    74281 gccaccacat ccacgccttt actacgccag gtctcgttag cgtaagcttc ctgggttgat
    74341 ccctgcagca caccaacatg tttacctttc agtgaatcca gcgttggctg aatcggtgaa
    74401 cctttggcag caatcaaacg agaatctgcg gcgtacagct tgtcggagaa ggcaatctcc
    74461 tgctgacgtt tatcggtaat ggaaagcgac gaaataatag cgtcgatttt tttcgctttc
    74521 agtgagggga gcagcgcgtc aaagtcactg gcaacccagg tacatttcac ctgcatccgt
    74581 ttgcacatct cgttaccgag atcgatatca aagccaacaa aatcaccttt agcatctttc
    74641 gacgagaacg gcgcataggt ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca
    74701 tagctggaag ccgctgcgga gagaccgact aataaagaca gagcgagaat cgacttcttc
    74761 atacataacc ctcaagtgaa aatggctttt ttatgttgtg tattgtgttg tgtgtttgca
    74821 ggctctttca tgcaggtctt atgccatctt gacgacaaca gtaacattca acgttaaata
    74881 tgttaataag acgttgcatt attgtcctga agttgaagat agcaggtata gcggttgaat
    74941 cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg atttgatcgc ggttgcaaaa
    75001 ttgccctgaa acagggcaac agcggagtta tgcgccctgc cagcgggcaa agagatcttc
    75061 aggaagggtt attgcaaact ggtcaagaac acgattaacc gtctgattta tcacatcatc
    75121 aagggattgc gggcgatgat aaaacgccgg aacgggaggc ataatcaccg caccgatttc
    75181 tgccgcctga gtcattaaac gcagatggcc taagtgcaat ggtgtttcac gcacgcagag
    75241 caccaacggg cgacgctctt tcagcaccac atctgccgca cgggtcagta agccatcagt
    75301 atagctatgg acaatgccgg acagggtttt gattgaacag ggtaaaatca ccatccccag
    75361 cgtctggaaa gaaccggaag agatgctggc ggaaatatcg cgcgcatcga gcgtgacatc
    75421 agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg agggataagg tctggcgcgc
    75481 cgcctggctc atcaccagat gcgtttcgat atctgtgaca tcgcgcagaa cctgtaataa
    75541 gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct acaatgagtc gtttcataaa
    75601 tagttgccct gtcagacttg caggcagact ttgcagtatt tcggcgggag ttgcaagtca
    75661 gggtgccaga ccggcaccct cagcgaaggc atcatccttc gttatgcatt tcgagatttt
    75721 ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt acgtaacgta tcgaggaaat
    75781 cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc gttgaacacc gagcattcaa
    75841 actgctggat atccggattt tcagcgcgaa cggcgtcgat cagatcgttc agatcctgga
    75901 aaatcaaccc gtcagcaccg atgatctggc gaatttcatc aacttcgcga ccgtgagcga
    75961 tcagttccgt ggcgctcggc atatcaatac cataaacgtt cgggaagcga atttccggtg
    76021 ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg tgccatctcg ataatctgct
    76081 cagaagtggt gccacggacg atggagtcgt cgaccagcag gacgttttta tcgcggaact
    76141 cggcgcggtt ggcattcagt ttacggcgca cggacttacg acgcagctgc tggcccggca
    76201 tgataaaggt gcggccaaca tagcggtttt taacgaagcc ctggcggtac ggtttgccca
    76261 gaatacgagc aatttccagc gcgatatcac acgaggtttc tgggatcggg atcaccacgt
    76321 cgatatccag atcttcccat tcgcgggcaa ttttctcgcc cagtttcgtg cccatattca
    76381 cacgcgcgct gtaaacggaa attttgtcga taaacgagtc cgggcgggca aagtatacat
    76441 actcaaacag gcacggattg ctgaccggat tgtcagcaca ttgacgggta aacaactgcc
    76501 cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca
    76561 gcgtatcgag cgctacgctt tcggaagcga ccatatattc tgtacggttc tcgtcaatat
    76621 cacgttttcc cagtaccagc ggacgaatcc cgtttggatc gcggaaagca accataccgt
    76681 ggccgataat catcgccaca caggcatacg cgccgcggat taagcggttt gtggcagcaa
    76741 tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg gaagttgtcc agctcgctgg
    76801 cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt
    76861 caaacagttt tttacgcaac tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa
    76921 tgccatacgg ggagttaacg taaaacggct gcgcttcaga ggcgctggag ctgccagccg
    76981 tagggtaacg cacatgacca atgcccatat tgccctgcaa acgctgcata tggcgagctt
    77041 caaatacatc gctcaccagc ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta
    77101 tggtgatgat gccggcggca tcctgaccgc gatgctgaag caccgttaag gcatcataaa
    77161 tcgactggtt aaccggcata acaccggcga taccgacaat accgcacata cgtctttttc
    77221 ctcgttaagc cacatctcag agcacttacg ctctgggcaa gaaacttgac gagctttgca
    77281 gataatcaaa aaaccatctg atgataaaac tgaattgcgg gatcagctgt gatttgctcc
    77341 agtcttcact tttcgacacc ccggtaaagg agtcgagaaa gaagagaatg gcggcaacaa
    77401 tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc
    77461 ccgttttctc caccaactgg cctatcacga agttcacgat agcaccaacg atcagggtag
    77521 cgataaacag taccgcgatg gcaatcccat ttcgaaccag ttcgtcttca aagcccgtaa
    77581 accagactga caggtaagtg tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca
    77641 ccagcgataa cgcttcacga acaaagccgc ggatcaggct aaccagagag gaaaaagcaa
    77701 tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt cccacgattt tacgccctgt
    77761 catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc
    77821 cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc catcgacttt ttatgccttt
    77881 gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc ttatccgacc tacggttcta
    77941 acactgccca agcctgataa gacgcgccag cgtcgcatca ggcaagactg tgttaattcg
    78001 gcgtatagcc cattaccacg ccacttaagc cagaaagttg cttcaactca cccagcgaac
    78061 ctttcagctt atctttcgag gcatccggcc caaccagaat acgggtaatt ttaccctgca
    78121 ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta ccgacaatct
    78181 cattcacttt atcggcattt ttcagcgcac ccagttgcac aacataggct ttacccgttg
    78241 gcgctgcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt ggtgcttcaa
    78301 cctttggttt aggcggctcc accggtttcg gcttcggtgg ggcgaccggt gcaggttccg
    78361 gttcaaactc ggtgttattg gctgcaatag tggccggatc gagcgacggt gctgcggcat
    78421 cacctgcccg cacctcttcc gctgcgcctt ccggcggctg cgtcggcaac gcctgggtgg
    78481 cggctggcat catatcaggc tcatcacgat cgcccgcttt cggcaccagc gggatagccg
    78541 cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt acaatcaccc
    78601 ccagcgccac cagcacgatc gtgcccacta accgattctg aaacttactt gccaccgctt
    78661 ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt ggaaagaacc acacaccagc
    78721 acggtgtctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc aacgctatca
    78781 aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc gcgcggccct
    78841 tccagtggcg cacaatacca gtcatcaacc acgcttttca accaggccag agttccggca
    78901 atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt cggtagcgct
    78961 ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc aaaaataacg
    79021 cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc aatcccgtcg
    79081 cgaatggcat tttcactgac ttccagcccg ctggcacgca gtgccgccag cgccgttgcg
    79141 gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg agcatcgcta
    79201 aacgcccagt catgatcggt gacggaatag ttccactcaa cgccccgacg ttgtaacagt
    79261 gcaccttttt cctgcgccac atcagcaatg gtagaaggca tttccggctc accgacaatt
    79321 gccggttttt cgctgcggaa gatgcctgct ttctcgcgac caatactttc gcgatctgga
    79381 cccagccagt cggtatgatc cagcgcaata ctggttacta ccgcggcatc ggcatcgaca
    79441 atattggttg cgtccagacg accgcccagc cctacttcca gaatcaccac gtcaagttgt
    79501 gcctgcttga acagccacaa cgccgacagc gtaccgtact cgaaataggt cagggaaata
    79561 tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc cggcaattcc
    79621 cggccctgca cacgtacgcg ctcggtataa cgcaccagat gaggcgaact gtagacgccc
    79681 actttgtacc ctgccgccat cagaatcgac tccagcgtac ggcaggtggt gcctttgcca
    79741 ttcgtacccg caacggtaaa cacaaatggc gctggtttca ggacgccaag acgcgccgcg
    79801 acctggctca cgcgctcaag gccgagctcg atagttttac tgtgcaggtt ttccagataa
    79861 gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat catggtatcc
    79921 gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc ccttatcagt
    79981 tatcaggcct caggttcctg atccggtacc gggggtacca ctacgccttc acgcggcgct
    80041 tcaggattcg gcgctggcag attcatcaac ttcgccagaa tgctcgccag tttcaggcgc
    80101 atttccggac gacggacgat catgtcgatc gcgcctttct cgatcaggaa ttcactgcgc
    80161 tggaatctag gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg accggcaaag
    80221 ccgattaacg ctttaggttc agcgatgttg agatcgccca gcatggcgaa acttgcagaa
    80281 acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg gcaagccgcg ctcctgcatt
    80341 tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc ttcctgcata
    80401 cgtgcgccac cagaggcgga gaagcagatc agcgggcagt tatcttcctg cgcctgctca
    80461 acggcacgca cgaaacgtgc acccacaaca gaccccattg aaccgcccat aaaggcgaac
    80521 tcgaatgccg cagcgacaac cggcattcca tacagagtgc ctttcatcac caccagcgca
    80581 tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt cttggagtca
    80641 cgaaacttca gctcatcttt cggctcaagc tcgctaccca gctccacaag gcttccttca
    80701 tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc acacttcgga
    80761 cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca gctatcacac
    80821 ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt gcttttaatt
    80881 cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc cagttttatc
    80941 tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc acattaaaac
    81001 ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cggagttact ttttattttg
    81061 cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat gacgcccggc aaaatagaaa
    81121 ccacaataat cccgacgatc agcagcttaa ggttatcctg aaccatcggg attgtaccga
    81181 agaaatagcc tgcgtaggta aaaagcagta cccacaacag tgcgccgatc acgttataag
    81241 cagcgaaatg acggtacgac atgtggccca ttcccgcaac aaacggggcg aacgttctga
    81301 cgatcggcac aaaacgggcg agaataatcg ttttgccgcc atgtttctca taaaactgat
    81361 gggttttgtc gagataactg cgacggaaaa ttttcgaatt gggattactg aataactttt
    81421 caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc cacaatcgcg gcgatcagca
    81481 tcagcactac catcatatgg acgttgagat cgttggtttc cagtgatgcc aacgctccgg
    81541 caacaaacag caatgaatca cccggtaaaa acggcattac caccagaccg gtttcacaga
    81601 acaaaattaa gaacaaaatg gcataaaccc agacgccgta ctccgcgact agctctgcca
    81661 gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc atatttgcct
    81721 atgccttgta ctcgtcatct ttcaggctgt aactacgttg gctacagcct gaaatgttcc
    81781 gagcattatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt tggaagatca
    81841 taccggtcag ggtaatccac cgcgaccaga tacagccctt ccgcttttgc cgttgctgcc
    81901 gccagcgttc tgtcctttgc cgccagcaac tctgctatcc agctctccgg ctggttgtgg
    81961 gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac aaaggcattc
    82021 gctttgatat caaccaccac ataaggaccg tgacgcgtga cattaatatg cataacgttg
    82081 cgccacgggg ttcgggactg gcactgcacc gcacggaacg aggtgaaatc attctcgccc
    82141 agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta aaaatgggtt
    82201 acccctttac tcagtaccgc cgggcgcagc cgatgattgt agatgatgta gcgataacgg
    82261 cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac ccaacgcacg
    82321 gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc acgccgcgtc tttgcgctgc
    82381 gcggttgttt cgaaatgcac cacttgcccg gtaccgtgta cccctgcgtc agtacgcccg
    82441 gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga gcgccttttc cagcttctcc
    82501 tgcacactgc ggacttcatt ctgccgttgc cagccgtaat acttactgcc gtcgtactca
    82561 atgcccagcg caattttata aactggcagt tgttgctggt cggacattag tacagatact
    82621 cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag cgaacgttat
    82681 cggccaccga ccagagctgg acttgctccg gcataccgta gtcattacgc acgcagccaa
    82741 cagaaagatg cggcgtaccc gaagcatccc ctacctgagt cgggaattcg ttctcttcag
    82801 agagcacaat atcttcgccc tgagcaaacg catcacgcgc ttcttctgcc gccagcggac
    82861 gcagtgcttc aaagttgacc atctgggcat gaccgtagaa taccggtgcc tggacgacgc
    82921 ttgccgaaat catcagccct tcgtcctgca ggattttgcg tacttcgtca acgatacgac
    82981 gttcttcacg cacgctacct tcgctatccg gcaacaacgg cagcatattg aacgccagtt
    83041 gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt gagcaatttc gcactctgcc
    83101 ccgctaacgc atcgaccgct tttttgccct gggcggaggc tgaaatcagg ctggtaacac
    83161 tgatacgcga caaaccgccc tgatcgatta acggtttcag tgccgccagc agctggctgg
    83221 tcagactgtc tggtacggcg atgacattcc ggttgcggta atcggtcagt acaaacgggt
    83281 ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacagtccg ctgctgtcga
    83341 tcaccaggca acctgagttg gtcgcttctt caacccaggc agcggtagct tctttgcctg
    83401 cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc agcggcatcc tgcacggtga
    83461 ttgttttacc accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc agtgcataaa
    83521 tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg cccatagcgc
    83581 cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac tccagaaata
    83641 gcaaagctcc ctgccaatgt tacagcagag agcatgaaga agagattaac gtgccggatg
    83701 atgaacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt catcacaaat
    83761 tacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagtt tatcgaactc
    83821 acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg cggcgcacat catacaccaa
    83881 atgcaccagc cttttcagcg tcggttgatc gagcggacca tgcagcgtaa tgcggccaaa
    83941 ctcaggcgcg ggcagcaacg tatccagcgc aacgtgttgt tcatgcccaa taaacttgct
    84001 ataggcttca aacacttgcg tcgtgccacg cgctttaccc tccaaggtat agcctgcgat
    84061 atgcggcgtg ccgatatcca cttttgtcag caattccaca ttaagttccg gctcgccttc
    84121 ccagacatcc agcaccacgc ttaacttctg gccttcattc aggcaggtca gcagcgcggt
    84181 attatcgacg actgcgccac ggcaggcgtt aatcagaatc gctccgggct tcaggctacg
    84241 gatcagtttt tcatccgcca gatgtagcgt tttgtacgga ccatctttaa agagtggcgt
    84301 atggaaagtc agaatatccg cgcgctggac taactcatcc agcgagcgga aatcaccctc
    84361 atccccacgg tcggcgcgag gcggatcgca aagtaaggtt ttaatcccta acgcttccag
    84421 tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg atccccaccg tacggtcgtg
    84481 cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat attccaccac
    84541 cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt cccgcctgct tcaaccatgc
    84601 ttcatcgaca tggtcggtcc ccgcagtggc agttccaaca aatttaatgg gttttcctgc
    84661 cagcaaagat tcattcactt tcgtgaccga acgcaccatc agcgcatccg cgtctgccag
    84721 ttgagcgacg gggattggac gcccgggaac cgcgatcacc tcacccaaac ggctaaataa
    84781 gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt tacctgtatg
    84841 agacgagagt taaccggaca agtgtgccat aatctcgcgg ccaggcatac ttgcgaagat
    84901 ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctctt gggcagccac
    84961 caggtcaggg agataaccta ccgtctggcg cgggcaatca gcctttatcc agtcagcaac
    85021 gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacaa caaagagcag
    85081 aactgtgggc gggtatcagg cacgatattg gtctgtcggg agattcaccg ctgctttcgc
    85141 gtcacttccc tgccgctgag cataatctgg cgcaacgtct gctggccgcg caaaaaagcc
    85201 attctgcccg ccagctttta gcgcaattag gggagtattt acgtctgggg aataatcgtc
    85261 aggcggtcac ggattatatc cgtcataact ttggtcagac gccgctgaat cagctctcac
    85321 cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt attccgcaac
    85381 cacagcagcg cgaggcgacc gaccgtcctt tattaccggc ggagcacaat gcgctaaaac
    85441 agctggtgac caaacttgcg gcggcaacgg gggaacccag caaacagatc tggcaatcga
    85501 tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg tttaaccatc
    85561 tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgccg acgctggaat
    85621 cactacagat ggcgctaaaa caacctttag atgccagtga actggcggcg ttatcggcat
    85681 atatccagca aaaatatggc ctttctgcgc aatcatcgct ttcttctgcc caggccgagg
    85741 atattcttaa tcagctttat caacggcggg ttaaagggat tgatccgcgt gatatgcaac
    85801 cgctgcttaa tccttttcca ccgatgatgg acacgttgca aaatatggca acgcgtcccg
    85861 cgctgtggat actgttagtc gcgattatcc tgatgctggt ctggctggtt cgttaacccc
    85921 gacgaaatga cagtatcgtg acaataatac ccagcaccgc agagatcgcc ccggcaagaa
    85981 ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc cagcggcccg gagacgccga
    86041 gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaact tgtgagggga
    86101 cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg gctccggtta
    86161 acgccgcgcc cgctaatgcg acccatgcac ctggggcttg ccagagcagc aacaagccca
    86221 ccgtttctac aagcagagag acaatcgcca ctttcacgcc gccaaaacgg tccggcatcc
    86281 agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga gtaaagcccg
    86341 ccatcgccca tcctttgctg gcaaagcaga gcgaaacgaa agtcccgata accgcaaaac
    86401 caacgccttg tagtgccagc cctaaccctg gtttccagat aagcccgaca acgctccaca
    86461 gcgatggacg ttctcccgcc agggccggta ctttgcgcac tgtgccgtta caggcccacg
    86521 ccagtaaggg taataccatt gtggtgatcg ccagtgcggc aaaaccgtaa tggctatgaa
    86581 tcaacaggcc aagcggcgca ccaacagcga gggcaccgta aatcgccatt ccgttccatg
    86641 acatcacttt gccagagtgt tttggcccta cgatgcctaa cccccaggtc agagcgcctg
    86701 tcagtaactg gctttcacca aacccaagaa tcaaacgccc gatgaccaac agggcaaatt
    86761 tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa cgcgccgcca gccagaccac
    86821 aagctaacat cccctgaagc gccgaacgtt ttgcgccata ttgatcggcc agtcgcccgg
    86881 cgtaaccacg cgtcagcacc gtagccagaa actgaatccc gacggcaatg ccgaccatgg
    86941 tattgccata gcccagatca tgatgaacaa acagcgggat aaccggcaac ggcaacccta
    87001 cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa gagcgaaaaa ctggcagaag
    87061 atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag cgcaaggcga
    87121 ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca ggatttaacg
    87181 cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt tgcctagaac tggtggcgat
    87241 tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt gcgttagtgc cggaggccgc
    87301 attggcgcgt aacgtcggat gcgacgctgg cgcgtcttat ccgacctact ccggcctact
    87361 gcgaattaat ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag
    87421 ctgttagaca taacagtggt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc
    87481 gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc
    87541 atcagcagag agtagatagc ttcctgcaca cccgcagcgc ccagtgagtg accggtcatg
    87601 gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagc
    87661 tctttcacgt cgccaaccgg agtcgaagta ccgtgagagt tcaggtaatc gattggggta
    87721 tcaacgccat gcatcgccat ctgcatacaa cgtactgcgc cttcgccaga cggagcaacc
    87781 atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat gtaagcacca
    87841 cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg
    87901 aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta
    87961 gtagacagcg cacccatcgc gtcgaattcg caggccattt cccagcacag ctcttcgccg
    88021 ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata
    88081 cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac
    88141 ggcgtggcga gacaggcaga aacgccggat gccatcgctt tagtgaccac atacgggcca
    88201 accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg gaaacgcggg
    88261 gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga
    88321 gagaggcccg catcagcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg
    88381 ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt tatgttgccc
    88441 cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt
    88501 ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc
    88561 aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt
    88621 tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag
    88681 aaatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac
    88741 ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc
    88801 tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg
    88861 ttatgttttt ctgggaggta accaattaga ggtacgcttt cctgagcatc cacatcctct
    88921 gtttgtggta gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc
    88981 atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag
    89041 ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga
    89101 actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca
    89161 tcgtttattg ctcgatgaag gccgcgtgac gctggattta tggtttggcg atattaacga
    89221 actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg
    89281 ctttgcgcca gctaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag
    89341 gttggcgcgt ccaggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg
    89401 tttgcaggaa gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat
    89461 gctttgtggg gtgatggaac agacattacc actcccctgc tccacgccgt ggtttaatcg
    89521 tacgggcagc agcaaacggg aagtagcgat tatcggcggt ggtattgcca gcgcgttgtt
    89581 gtcgctggcg ttattacggc gcggctggca ggtagcgctt tattgcgcgg atgaagcccc
    89641 cgcgctgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga
    89701 tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca
    89761 attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa
    89821 aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc
    89881 cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac
    89941 ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc
    90001 gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga
    90061 tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact
    90121 ggcgaacggg catcaaatca gccgtttcag ccaaacgtcg actctcccgg tgtattcggt
    90181 tgccgggcag gtcagccata ttccgacaac gccggaactg gcagagctga agcaggtgct
    90241 gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc
    90301 cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg
    90361 ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga
    90421 taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa
    90481 tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc aaaaagatga
    90541 ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg
    90601 tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat
    90661 tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg tgcggaaatt
    90721 gttgaagggt aaagcggtta aggcgcggta atctgctctc gcagtgtttg ccggatgcgg
    90781 cgtgaacgcc ttatccaacc tacgtgtgac gtgtaggcct gataagacgc gataagcgtc
    90841 gcatcaggca tggtgctcca gacgccgcaa cttacagctg tgacgcctgc tgaaacaggt
    90901 tttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg gcaccaatgg
    90961 ctttttccag actctggcta acggtcgtat gcaccgcctg agcggagtgg tcatcaccac
    91021 tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact
    91081 catcatcact ggcatggtca accatgccgt cgattaatgc cagaatgcgt gattcaaact
    91141 ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg agcgcttccg
    91201 gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa
    91261 tgaagcgtgc cgggcgttcc ggaatgccat ttgtcagata atccatcacc tgcgcatgta
    91321 cccgccgttg aaacaccaca cgatccggtt cgaaatcccc ttccagattg tcgcaactga
    91381 cgttaaacgg atatccagcc gccacgcaga ataaccagtc gaacgcctgc ggcttcactt
    91441 caacatcttc aaactggctt tgcgtctggg catcgcgtcc gtccgggcaa taccagtagc
    91501 cgaaatcaac cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa atctcatgaa
    91561 ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt atacggtact tccgcatcag
    91621 caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat tcatcggcaa
    91681 agcagctatt aaaaatttca atcaactgct cgtagtggtg tgtactgttc attagttcat
    91741 ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg cactcatcac
    91801 cgccgagacg ataacaatca tcgggcggat cagcttttgt cctttgctca acaccaaccg
    91861 tgaacccatg cgcgcgccga ggaactgccc caccagcatg acaaagcccg tcgcccaaat
    91921 cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg cgttgagtaa
    91981 tttggcgtga gccgtggctt tggcgaggtt gaatccgcac agcataacga aggccagcgc
    92041 gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc agccaccggc
    92101 aattaacgca aagggtaaac cgtacatccg gcgctggcga tcttcttcac ccagctttgg
    92161 catcagcaag aaatagaggc cgatacaaat caccagaatg ggcaaaatct gccgtaagac
    92221 atcagcctga acgtactgta ccaacagtgc gccgctcatt gagccgacaa aggtcatggc
    92281 aatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc
    92341 ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag cgggagacat
    92401 tcccgcagcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat
    92461 aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcggag aaaccataaa
    92521 caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc
    92581 ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg aggttcaaac
    92641 cagctttgca gttctgcccc gcaaccatcg cctgatggcg gtaaaggttg atcttcacac
    92701 tccagactat cggcagggca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag
    92761 ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta
    92821 atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag tttgatcaag
    92881 ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg ggaaactaag
    92941 tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg cagttgcaga
    93001 aagatatcga catccagtcc ggtctggtgg ctggcatgac cgccgttgaa acgcccacca
    93061 gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcta
    93121 ctcagacgct ggataaacat caccagatcc gggtgaccga aatagcgacg ctgatcggta
    93181 cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcgccgac aatacagcca
    93241 ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac
    93301 ggcgtcgctg ccaggctagc gctactggca agcagagcca gcagcgcaat cgcggttttg
    93361 ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt
    93421 aacaggtgat ccattaaaac gatcgccagc atcgcttcag cgatcggcac tgcacggatc
    93481 ccgacacagg gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggtta
    93541 atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatatg agcaatgatt
    93601 tgctgcccgc tgctgatacc gccgaaaatg ccgcccgcat ggttgctctg gaaaccctct
    93661 ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa accgtcgcca
    93721 atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg
    93781 cggtcaaaga ccggctcgcc aagtccggcg gggacaccac tggcaacaac ggtgactttc
    93841 gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt
    93901 ttgtcggggt ccgggcagaa gaacggattt tgttcaacca gcgaccagtc tttgatttcc
    93961 agcggaatgt cgcccatctg ggtcaggcag ccgcggattt caataccaaa tttctcggcg
    94021 agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa
    94081 cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcagcatgg
    94141 cctggacgga agacgtcttt aattgcgctg taatcctgag aacgctggtc ggtattttcg
    94201 atcaacaagc caatgctggt gccagtagta acgccttcaa aaacaccgga gagtattttg
    94261 acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg acgacgatca
    94321 aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg
    94381 cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt
    94441 gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata
    94501 aatcgcgaaa tgttctcgtg cggcaagaag ctgctcttta gtgagcataa acacaccatc
    94561 gccgccgtta tcaaactcca gccaggtgaa cggaacatcg ggatattgtt ccataagatg
    94621 taccatgctg ttgcccactt cacaaatcaa cacgccatca tcagcaaggt aatctgccgc
    94681 gttaccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca ggcccagttc
    94741 cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcgtcga catacggcgg
    94801 gttagtgaca atcagatcgt attgcacttt cggcaaatca cggaacagat cggaacgaat
    94861 cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa ccgccagcgc
    94921 gtcgggagag atatctaccg cgtcgacttc cgcttccggg aaggcataag cacaggcaat
    94981 ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct tgctgatgag
    95041 tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc
    95101 atcgacgtaa aattcatgac cgcagaacca ggctttgttg gtcaggtaag ccaccggaat
    95161 gcgctcgttg acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag
    95221 acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagcgatg gtaacaccag
    95281 ctgtacggct tcatcccacg ggttatcggt gccgtgaccg taccagatat ttgccgcgct
    95341 gaagcggctt accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc
    95401 atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct
    95461 agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg
    95521 tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac
    95581 agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg
    95641 atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaaaaaa
    95701 atcagcgaag tgccggtgaa acgcttgatc caggagcagg ctgatgccag ccattatttc
    95761 tccgatgagt ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt tcgcccggat
    95821 gtcagccatt ttgaggcgaa gaaactgcgc cgcggcgatt attcaccgga attgtttttg
    95881 gatttacacg gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt gattgccgcc
    95941 tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa gcatattttg
    96001 aagcaacaaa caccgctgtg gctggcacaa catccgcatg tgatggcatt tcaccaggca
    96061 ccgaaagagt acggcggtga tgcggctttg ttggtgttga ttgaagtgga agagtggttg
    96121 ccgccggagt tgccctgaat gctggcagca aatgccggag cagcgtaaac gccttatccg
    96181 gcctacaaat atgtgataat tcaataaatt gcaattcact tcgtaggcct gataagacgc
    96241 atcagagcaa tactacgaac ttcagttcat tcaatacata ctgaaaacga cacgtcgaat
    96301 caatcggttg cccactactg gtattcacat tcaccacaat atcgtgtagt actttatccg
    96361 ttaccggatg acgagttggc gtaaccgtta ccgttgattt atcacaaccg taagaggtaa
    96421 aggttaacaa ttctgatacc gcgcggcagt atgctcctct gccatcatta tcagaaaaat
    96481 tactgccatc acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg gtggtcactg
    96541 ttgaccccgt ggactgaatt cgtaacgcct tattatggct gacataattt tcactccagg
    96601 agatatcgta tgaagcgacc ggtatcgttg ggcatattgt gttatgcact gtaggggccg
    96661 tatagcttcc aaatccgggt aaatctgtat aggaataaaa ggatgcgatc caagggcaac
    96721 cataatcatc agatgcaacg gtaattttaa acgtcatata tgcacaacga aaaattttcg
    96781 tatctatgcc agggcaattt gagttattaa ttgtatcaaa gtcagtcagt accaaagttg
    96841 gagctgaacc cgacccagac caggaaataa cccgcaacgt atcgccttta tacgcaaaag
    96901 aatcatattt cccatatgca acctcctttg gcgttatcac cgtaacattg aaaacactgc
    96961 tcgctacagc accggttacc ttgtccatgg cataataaac tctcacactg tcaaaactcg
    97021 cactcataag tttatctcca gcataactct tagatgcgcc gacaacactc agaacgagca
    97081 gcattaggct cgcgaaaatt atggtccatt ttttcatttc agaaatactc aaaggttgcc
    97141 gtcactgtgg cactaaagtc tccggacgtt acatctcgcc cgctctttgc ctgtaactag
    97201 gtattaaaat ttaggctgtt atttccctga ttcagcggga gcgtgacgcc tgttgtattg
    97261 ttgattggca ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc ggatgctgat
    97321 gacgaggagt caaaggccag aaagccgggt agagcactat cttctgttcc tgtcaacgtt
    97381 actttgacat taaacaatgt agaactatga caatctttca attgaaacac caatggcact
    97441 cgctctgact gcccaaggtt cattaaatcg ctggcggcaa tggcaggaaa atgtacttct
    97501 gccaactggc taccattaat cactggcgta cacgatttac tgattaaatt gccggtaaag
    97561 tgcaaattat ctcctgctat tgtggttcca ctgcccagca acatcaggct actaataaaa
    97621 aacaacgtac gctttttctt cattggtaat ctacctgtaa cgtggcccag gcctcaaagt
    97681 ccgcctctgg cagaatgaca ccactctttt ttactggcac agccgttaac accggtaaat
    97741 cggtttcatt caccacaagt ggcgtattga tcgtaaaact ctgccctgcc tgtttgagct
    97801 gaatgcctaa accagtgata ttgctgctta ctgcagcatt atcaaaatcg cttggtgtgc
    97861 cactccagct caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg caggtaatgg
    97921 aatacggcac ggtttgctca taattgctgc cattaatgtc atcaataagc acatcctgaa
    97981 aagttatttc aatggtttga gcgttgctaa tcgtgcagtt aggcggctgc actaaataac
    98041 cgtgaaaaga gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa ataagcgaga
    98101 tccgtttcat tggtaatcca ccacaattgt ggcactggcg ttaaattcag ctgccgctaa
    98161 cgttgtacca ctctgtttca ccggcacagc ttccagaacg ggaactcccg aggaaaaatt
    98221 aaatggcaac catgagccag aggttaagtc gagtattgtg tgatcactac ttttctgaac
    98281 ccgaatacca aacccaggaa tacccgtgct gataactgtt tcaccgttaa tcacgaccgt
    98341 tgtcgcctgc atctgtagac gcaggtcatc cattgccaga ttgttacaaa caagcgagta
    98401 gtcaatggga cgtttataat caaccctatt gatcttatta atgaatacat taccaaactc
    98461 aactgccgca ccatttaccg tacaaggagg aggggcatcg acaagcaccc gtaaattcat
    98521 acttactgac ttactcgctg caactgcaaa gccgctacca acacaacata tcagtaaccc
    98581 agttaccgcc cagacagaac aagatccttt catactggct ctcccggcct cagcctttgc
    98641 ggctctgctc ttcatcgacc tagcaactgt taccgttgca agtgaaaaag agcggcatcc
    98701 gagcgccata gtcgttgaca taggtcagta ccggcacgct acccatcttc acattcagcg
    98761 gtgcagaggt tttcggcgac atcactattg gcgagaaacc ggctgccgga ttgccatttt
    98821 tctgtgtgct ggcattactg aaaatgacgt aatacgacgt cgggttattg acggtaaatt
    98881 cctgaccgtt gcgggttaaa gtaaccttat attgataggg gtttttcatg ctgacgttct
    98941 ccagcgcctt cggtcgccag aacaacttaa tacgcgtttg tagtgctatt tgcagcgtgt
    99001 tggctttgtt tgattttggc gggatctcac gcacgttgaa gtagaacagg ctttcacgat
    99061 cggcaggcaa tttgttgata tccggcattc cctgcacttt tacctgcccg ttcatcattg
    99121 aatcgatgcg ttgcaccggt ggcagaacag ttaacggtga actgatcttg ttaccctttt
    99181 catcttcgat ccagctttgg gccagataag gcagtttcgg atcgttattg cgcaacgtca
    99241 cgctgattga tttgtcgctt tcattaaata tcaaacgggt acgatctggc gttacagaag
    99301 cctgggcaga caaactggca gcgctaagca gtaacgctag cgtcgtagca cccaatttgg
    99361 cagagcaaag taaatcagac atggatcatt ccttaatcac gttattggtt agctgaaaca
    99421 gaaacagggg gattggtggg tgtcacctgt tgcgtctgct cctgaatgac aggctttatg
    99481 tcatcaggaa caaccggcgc gacgttgcct ttatgctggc atggcagtaa caagccgtta
    99541 aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac accactccag
    99601 aacacactca tgtgttcgcc aggtttcacc ccagccagat aaacattgcc gtcatcatcg
    99661 acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg aggatggctg
    99721 ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc aaatttgcgg
    99781 tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc ttcggcgttt
    99841 tccggcagtt tgttgaggtc gatatacgcc tgattgcggt aatagttatt gacgtcagaa
    99901 acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc taccggaaca
    99961 tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg ggtacggtga
   100021 agtgcgccac cgtgggcagt aaacgttgcg ccgccctgta acgacaagcc cgcagaggtg
   100081 tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc atcatggctg
   100141 taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg atagtgagtc
   100201 gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact gccatagtac
   100261 cgttatagct aacggtactg ttgtcgcccc acggcatact gagcgaaatg tacatgcctt
   100321 tgtcggcctg gttgtcatac tcgtaacggt agccagtcat cgagatgctg acattacgaa
   100381 tactgcccat attgaaatag tgcgagagca tgatgttgta gtttgtctgc tcctcgcgat
   100441 cccagtaggt atggcgggta tagttgagat aaaccgaaac acccgcatcg cggaagttct
   100501 ggttataagt ggcggtgtac atctctttgt cgttgcccgt gcgtaccatt ccgctgtcac
   100561 tggcatccag atactcgctc atggtcataa agttctcttc cgagaagcga tatccagcaa
   100621 aagtaacgcg gctgttgagc tggtcaaagt ctttggaata actcacacgg aaggagttac
   100681 cgtccagcga acctttgcca taagcggtgt ctttatccag tttggtgtgc gagtgagtaa
   100741 catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc gacaccaagc gccgcagact
   100801 gatagttttc atctcccagt gcgccgccat ataacgacca gccgttagcg atcccccagg
   100861 aagcttcagc accagaaaaa aattcaccct cgacatggtg tccccactct tgcggacggc
   100921 ccatcatgat cttatagcga acctgacctg gacgagtgag gtatggcatc gaggcggtgc
   100981 tgatgtcata ttcctgcacc tggccgttct gttcttcaat gcgaatatgc aacgtaccgg
   101041 agacagaatc accaagatcc tgaatacgaa acggtccagc cggaacctgc gtttcgtaaa
   101101 tcacacgccc catctggctg acggtcactt ttgctgtggt gtgtgccacg ccggaaatgt
   101161 ctggcgcgta gccgcgcaga ttgggaggca acatttgatc gtcagtactg acactgccac
   101221 caacatagtt aaaaccatca aaaatatcgg atctgaggta atcctcgccc agcgccagtt
   101281 tggcttttaa tgatggtaac gcccgcctgg catagtagcg actccactcc catttttttt
   101341 gagtttcatc gccgctgaat tcatcgtcat cattactgcg agtatgttga tagttagtct
   101401 gccagtcagc acgcatacgc cacggcccta ggttaacccc aaccgtcccg ttgccgctga
   101461 tctcgttact atcatcaccg ccattttctt cgtgccgggt ttgtgcgttg atgctgtagt
   101521 ccgcgacgat cccggagatg ccgtcatccc aacgtgaagg cggatcccag tcgggataag
   101581 tatattcgag gtaagcctgt ggcagtgaaa tgactaatgc ggactggctt aaatcagcct
   101641 taatttccat gccttccagt tgaccggatt tcaggcattt agcatcgtgg ctccattgca
   101701 gatttttcgc cacgtcttct tttaaaccaa actgcgctac cagttccggt gtcagacaag
   101761 catagctttt gctcgcgtca tcttcaccag cataccagta aatatcgtac tcttccgcca
   101821 atggctgttt atttagttga acctgtaaat tgtattttcc gggctcaaca tatccctggc
   101881 tggaaaaacg cttcagatca atttttgtgt cgccttttaa ttccagaaaa cgggaatcaa
   101941 actgaatgtc gtcttcagcc cagacactgc tataactccc tgacattgcc agcgcaatgc
   102001 accacggaag aatccgtaat cgaaaaagag aatggtcagg catacacgtt tctatccgtt
   102061 tttattcttg ttcggtcaaa atccatttaa atcccccata gcagtgtcgc tatgggggta
   102121 tgccttacag gtaagtaatc tggaaggtgg tatttgcttc aaaattaccc agatctggcg
   102181 catcagctgc gccgaccagc caggctttaa agttcagtgt ctgcttcgct ttgcctttgt
   102241 tggtagaagt gtctttcact attttctggt caatacccat gccgcttttg taagaggtac
   102301 ccagagagtc accgatcgcc aggctaacat tgttaaatgc cgcaccggta tcggtattga
   102361 aaatggtgta ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca
   102421 ttgtttcctg agtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg
   102481 tggtgccgtt atttttcagt ttttcggcac cgatatcacc catatcaact tccagtttgt
   102541 gatcgtccgg aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac
   102601 cgttgttacc cgcagcgatt gttgccgtag aggtgaacac gcccatcacc attgccgcag
   102661 caatagctgt tttaacaaac ttactcatta tttgtcctta aactataaat aattaaaaat
   102721 ataaacattg caatacattg attcagtcaa tagccaatgt ttatacccag ccattgaata
   102781 cggcatgcga cccaaaaaga acaggtcgta gaaaattgta ttcattggtg tggggccgca
   102841 tagtgatgtt tccacacccc tgatccaatt taattttact aaagagtaaa tgtagattta
   102901 attaatatat tgacagaaga aaggttatct taaggacatc cttaaaaaat acggaataag
   102961 tatttagaaa aaatagaaag tatatcaata atgaatatca acagcttaaa aaacaaacaa
   103021 tgaacatata atgcgattgg catttatcca cttttgacaa tataatatat aaacactata
   103081 gccagaattt aatcaagttt caattacatc ctgcctgatt attcaactct attttctcta
   103141 ttatctgtat taataaatga cactgagtgt caaacagaca ttattcattt caacatgtaa
   103201 ctttgggcaa aatcaagata cgcgcagaag aataatttcc gaatgaatgt agaaagtgtt
   103261 ttatttatag ataaggagag tcttcgatag tagtgaggtt gacagatatc cgctgcctaa
   103321 tgagcaatta tgcccaatga atctacctca ttgggcataa tttggaacac atcagatagc
   103381 ttttgccatc ttcagattac acgggctcat ctgccagttg aatgtaccgt ttccgctttc
   103441 atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt cgcccggaca
   103501 taactcggca accagatacc cgactaacgg taagtgagag atcaccagta ccgaagcaac
   103561 accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc cgcagggcgt
   103621 taactccggc agaacttctg cactggaagg caggttcaga caatccccta cttcttccag
   103681 tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca ctttttgacc
   103741 tttcagccag ttcgccatca ggcgagattc gtcacaacca ttagtggtca gaggacgaac
   103801 cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc atgataaaaa cttgcatatt
   103861 gcaccgcttt tgttaaccag attcgcccgt tttctttcat ctgaaaccag agaaagaaaa
   103921 cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt tacctcaatg
   103981 gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca ctccgccatt cagcgcggat
   104041 tcatatagct ttgaccttct tattgcaggt cagttgcagt tgttttccaa aaactttccc
   104101 cacgcgcgcc catctcgacc aaacgctcgc aaggggtaaa acgggaacca tactgcgtgg
   104161 ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata tagcggaacg
   104221 gtccaccgag aaatggcgga aaaccaatgc caaataccgc accaatatcc ccgtcacgca
   104281 cgctacggat aacctgctca tcaacacaac gtactgcttc attcagcatc aacatcacac
   104341 accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca atgagcgggt
   104401 aaatggcagg atcgacctgt tttttgcttt tacgcccttt ctgaccataa agatagaaac
   104461 cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca acatttgcag
   104521 gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta gtcccggtgt
   104581 cgattcctac ctcatccaaa agttggattg ggcctaccgg aaaaccaaat ttcactagcg
   104641 cggcatcaat gtgctctacc cgttcacctt cggtcagcat gcggatagct tcattaatgt
   104701 aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca atgggcgttt
   104761 tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgcgccgat gtacccgcat
   104821 gaggaataat ctctaccagc ggcatttttt ccaccggact gaagaaatgc aggccaataa
   104881 cttgctcagg tcgcgcggcg tgagcggcga tatcaccaat cggtaaagat gacgtgttcg
   104941 aagcaaaaat ggtatgagcg gcgcaatttt gctccacttc cgccaccatc tgttgtttta
   105001 attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag ccgcgatagt
   105061 ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg agatgacgac
   105121 ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt atgccctgcg
   105181 ggttgatatc tttaattctg accggaagcc ccgctttaca agcagtgaca taagcaatac
   105241 cgccgcccat caagccacca cctaaaattc ccacgctgtt taatggcgca ggcggtgcat
   105301 cactaccggg atctttcttc acgtccgtac tggcaaaaaa gatactacgc aaagcttgcg
   105361 attgcggcgt catcgccagt tcgccaaagg cgcgagcttc agcgtcatag ccgctgctgg
   105421 tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc ggataattgc
   105481 cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca cgacctaacg
   105541 gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga tccttcttcg
   105601 ccagctcaac agcggcttcc agcaaaatgg agtgcggaac aacgtcatcc accagcccca
   105661 gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag aatcatctct aatgctgtac
   105721 tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac aatccaagtt
   105781 gtacttcagg caaaccgagc accgttttgg gatcgtcagt acaaacgcga ccgtggcacg
   105841 ccagcgccag ctccagccca ccgcccaggc aagcgccatg aatagccgcg ataaccggaa
   105901 tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccagt gcttccgctt
   105961 cttgcgccgt tttgcagttg ccaatcatgt tgatgtctgc tccggcgata aaattgtccg
   106021 gtttcgcgga gataaacacc acgccgcgca actctttgtt ttcacggagt tgcttaataa
   106081 tggcgcgcac ctgcgaggca aattccgcct tcagggtatt cattttctca tccggtacgt
   106141 cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct gatgccattt
   106201 ccattattcc gcctccagaa ccattgccgc gccaagccca ccggcagcac aggcggtaac
   106261 taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa tcatccgcgc
   106321 gccggtcgcc gcgaagggat gtccgtaagc aatcgaaccg ccaagcacgt taaatttgct
   106381 atcgtccact tcgccagtgg catgtgcacg ccccagtact tcacgagcaa aacgttcact
   106441 acccagcaac tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca tatcgatcaa
   106501 tgtcagttcg ctcatcgtca aaccggcacg ttccagcgcc agcggtgttg accaggctgg
   106561 accgagcaac atgtcctgcc agacatcaat cgcagtaaat gcgtagctgc gcagataccc
   106621 cagcggcacc agccctaatt ctttcgcccg ggattcagtc atcaggatca ccgctgccgc
   106681 gccatcggtc agcggcgtac tgtttgccgc cgttaccgtt ccgtgtttgc gatcaaacgc
   106741 cgggcgcagc tttgcgtaat cggcaagcga ggaattaccg cgaatattgt tgtcttcgac
   106801 aagcggttgt ttataaggag ggataaaggc agtcatcacc tcttctttga gttttccgtc
   106861 tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt gctgttctcg
   106921 ggtgatgccg taggttttcg ccatttgttc ggcggtgtcg cccatccgca agccagtaga
   106981 atattctgct accgcaggcg gtacgggcat taagtcgcgc aaacgcaggc gagagaagag
   107041 tttcaggcgc tggctcatgg ttcgagcttt gttgacatca accagcacgt gcgccagttt
   107101 tttactgacg ccaattggca atacggaaga ggaatctgcc ccaccggcaa tccccgctcg
   107161 gatagttccc gccatcaggc tttctgcgac gtttgcaacc gcctggaaac tggtagcgca
   107221 agcgcggctg acgctgtaag catcggtatg cacattcatt ccggtaccga gaacaatttc
   107281 acgcgcaatg ttgggggctt caggcatttg tacgacctga ccaaagacca gctgttcaat
   107341 cacttcagcg gggatctcgc tgcgtgccag cagttcgcct accaccatct tccctaaatc
   107401 aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac gtaaaccgct
   107461 aacaatggcg atacgatcgc cctggcgggt aaccagtggt aaaacctgac ccataacact
   107521 cccctgtaaa aaaataaata aagtggtctg acctgatcat agtattaacc atttttttac
   107581 atttagccaa gcggagaaaa aggaaagtgg gagctatgac acagtgaaga cagaagaaag
   107641 aaaagaaaac gcccctgcca gcaactgaca ggggcgtacg tacccgagag gaattaacgc
   107701 agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg
   107761 cgcacaccgt cagactcttc agtgaaagtc ggggagattt tgcacggttc ggattccacg
   107821 ctacgggatt tttcagtcag cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac
   107881 acgcggctgt aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag
   107941 cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc
   108001 acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt
   108061 tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa
   108121 aattcacccg aatccgcgag tgcgcaacct ccaaattttg ccagctggat cgcgtttctt
   108181 agatcatatt tgaaaaaaga tggaaacata cttgcaacat tctagctggt ccgacctata
   108241 ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt
   108301 acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa
   108361 aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg
   108421 gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg
   108481 ggaaggcgca attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat
   108541 gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gacccggatg tgaatatcag
   108601 cggaacgtct ccgtctggtc gtagcctgca agccgataac atcgcgccta cggcatgggt
   108661 tccgaacatg cactttgttg caccgattaa cgaccaattt ggttggggcg cttctattac
   108721 ctctaactat ggtctggcaa cagagtttaa tgatacttat gcaggcggct ctgtcggggg
   108781 tacaactgac cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct taaataatgc
   108841 atggagcttt ggtcttggtt tcaacgccat ctacgctcgc gcgaaaattg aacgtttcgc
   108901 aggcgatctg gggcagttgg ttgctggtca gattatgcaa tctcctgccg ggcaaactcc
   108961 tcaagggcaa gcattggcag ctaccgccaa cggtatcgac agtaatacca aaatcgctca
   109021 tctgaacggt aaccagtggg gctttggctg gaacgccgga atcctgtatg aactggataa
   109081 aaataaccgc tatgcactga cctaccgttc tgaagtgaaa attgacttca aaggtaacta
   109141 cagcagcgat cttaatcgtg cgtttaataa ctacggtttg ccaattccta ccgcgacagg
   109201 tggcgcaacg caatcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcagg
   109261 ttataaccgt gttgatccac agtgggcgat tcactatagc ctggcttaca ccagctggag
   109321 tcagttccag cagctgaaag cgacctcaac cagtggcgac acgctgttcc agaaacatga
   109381 aggctttaaa gatgcttacc gcatcgcgtt gggtaccact tattactacg atgataactg
   109441 gaccttccgt accggtatcg cctttgatga cagcccagtt ccggctcaga atcgttctat
   109501 ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa
   109561 agatgcttca gtcgacgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga
   109621 aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta
   109681 cgcgttctga taacgcgttc gcctggataa agtcacctgc tatgcaggtg actttaactc
   109741 ccccacttca ccgaaagtag tttcctcatt gcttgcctcc tgagttttgc aaacagcctg
   109801 atggcagctt gccctttcaa atcagtaagt ggtatccaca gacaaacctg aagggaaatg
   109861 catattttca agcgttctgc tcgtccttct caaagagttt acttttctgc atttccagga
   109921 tactcaaccc ccctggctat tgtgcgctta tacactcaaa ttaaagatag gttctaaata
   109981 aatgagcgtt ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca
   110041 acatagaata aaatcatgct accatctatt tcaatcaaca ataccagcgc agcttaccca
   110101 gaatccatca atgaaaataa caatgatgaa attaatggat tagtacaaga gttcaaaaac
   110161 ctttttaatg gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa
   110221 aacgccatac gagtaaacga cgatccttat agatttaata ttaataattc ctcagttact
   110281 tatattgata ttggctccaa tgatacagac catattacta ttggtatcga caaccaagaa
   110341 ccaatagaat tacctgcgaa ctataaagac aaagaactcg tccgtactat cattaatgac
   110401 aacatagttg agaagactca tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa
   110461 gaaatatatg atggagatcc tggttttatc ttcgataaga tatcacacaa actcagacat
   110521 acggtaacgg aatttgatga gagcgggaaa agcgaaccaa cggacttatt tacctggtac
   110581 ggtaaagata aaaaaggcga ctctctcgct attgtaatta aaaataaaaa cggaaatgat
   110641 tacttatctc tcggttacta cgatcaggac gactaccaca ttcaaagagg aattcgtatt
   110701 aatggtga
//
LOCUS       DAJTYI010000002        97700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYI010000002 DAJTYI010000000
VERSION     DAJTYI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 97700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..858
                     /gene="pdeF"
                     /locus_tag="OO564_000108"
     CDS             <1..858
                     /gene="pdeF"
                     /locus_tag="OO564_000108"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6651383.1"
                     /translation="NTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEH
                     NHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEH
                     TLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNA
                     LTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNS
                     LDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLN
                     EIEPIRESA"
     gene            complement(897..2438)
                     /gene="ppx"
                     /locus_tag="OO564_000109"
     CDS             complement(897..2438)
                     /gene="ppx"
                     /locus_tag="OO564_000109"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6651384.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(2443..4509)
                     /gene="ppk1"
                     /locus_tag="OO564_000110"
     CDS             complement(2443..4509)
                     /gene="ppk1"
                     /locus_tag="OO564_000110"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6651385.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(4681..5319)
                     /gene="purN"
                     /locus_tag="OO564_000111"
     CDS             complement(4681..5319)
                     /gene="purN"
                     /locus_tag="OO564_000111"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6651386.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            complement(5319..6356)
                     /gene="purM"
                     /locus_tag="OO564_000112"
     CDS             complement(5319..6356)
                     /gene="purM"
                     /locus_tag="OO564_000112"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6651387.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6681..7307
                     /gene="upp"
                     /locus_tag="OO564_000113"
     CDS             6681..7307
                     /gene="upp"
                     /locus_tag="OO564_000113"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6651388.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            7393..8682
                     /gene="uraA"
                     /locus_tag="OO564_000114"
     CDS             7393..8682
                     /gene="uraA"
                     /locus_tag="OO564_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6651389.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            8732..9478
                     /gene="hda"
                     /locus_tag="OO564_000115"
     CDS             8732..9478
                     /gene="hda"
                     /locus_tag="OO564_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6651390.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(9611..9970)
                     /gene="arsC"
                     /locus_tag="OO564_000116"
     CDS             complement(9611..9970)
                     /gene="arsC"
                     /locus_tag="OO564_000116"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6651391.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(9991..11454)
                     /gene="bepA"
                     /locus_tag="OO564_000117"
     CDS             complement(9991..11454)
                     /gene="bepA"
                     /locus_tag="OO564_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6651392.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            11667..12728
                     /locus_tag="OO564_000118"
     CDS             11667..12728
                     /locus_tag="OO564_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6651393.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(12766..13614)
                     /locus_tag="OO564_000119"
                     /pseudo
     CDS             complement(12766..13614)
                     /locus_tag="OO564_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(13636..15648)
                     /gene="hyfR"
                     /locus_tag="OO564_000120"
                     /pseudo
     CDS             complement(13636..15648)
                     /gene="hyfR"
                     /locus_tag="OO564_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(15678..16091)
                     /gene="hyfJ"
                     /locus_tag="OO564_000121"
     CDS             complement(15678..16091)
                     /gene="hyfJ"
                     /locus_tag="OO564_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6651394.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(16084..16842)
                     /gene="hyfI"
                     /locus_tag="OO564_000122"
     CDS             complement(16084..16842)
                     /gene="hyfI"
                     /locus_tag="OO564_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6651395.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(16839..17384)
                     /gene="hyfH"
                     /locus_tag="OO564_000123"
     CDS             complement(16839..17384)
                     /gene="hyfH"
                     /locus_tag="OO564_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6651396.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(17394..19108)
                     /gene="hyfG"
                     /locus_tag="OO564_000124"
                     /pseudo
     CDS             complement(17394..19108)
                     /gene="hyfG"
                     /locus_tag="OO564_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(19098..20678)
                     /gene="hyfF"
                     /locus_tag="OO564_000125"
     CDS             complement(19098..20678)
                     /gene="hyfF"
                     /locus_tag="OO564_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6651397.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(20683..21333)
                     /gene="hyfE"
                     /locus_tag="OO564_000126"
     CDS             complement(20683..21333)
                     /gene="hyfE"
                     /locus_tag="OO564_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6651398.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(21345..22784)
                     /gene="hyfD"
                     /locus_tag="OO564_000127"
     CDS             complement(21345..22784)
                     /gene="hyfD"
                     /locus_tag="OO564_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6651399.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(22812..23753)
                     /gene="hyfC"
                     /locus_tag="OO564_000128"
     CDS             complement(22812..23753)
                     /gene="hyfC"
                     /locus_tag="OO564_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6651400.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            complement(23764..25781)
                     /gene="hyfB"
                     /locus_tag="OO564_000129"
                     /pseudo
     CDS             complement(23764..25781)
                     /gene="hyfB"
                     /locus_tag="OO564_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(25781..26398)
                     /gene="hyfA"
                     /locus_tag="OO564_000130"
     CDS             complement(25781..26398)
                     /gene="hyfA"
                     /locus_tag="OO564_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6651401.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(26651..27121)
                     /gene="bcp"
                     /locus_tag="OO564_000131"
     CDS             complement(26651..27121)
                     /gene="bcp"
                     /locus_tag="OO564_000131"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6651402.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(27121..27693)
                     /locus_tag="OO564_000132"
     CDS             complement(27121..27693)
                     /locus_tag="OO564_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6651403.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            27839..28717
                     /gene="dapA"
                     /locus_tag="OO564_000133"
     CDS             27839..28717
                     /gene="dapA"
                     /locus_tag="OO564_000133"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6651404.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            28734..29768
                     /gene="bamC"
                     /locus_tag="OO564_000134"
     CDS             28734..29768
                     /gene="bamC"
                     /locus_tag="OO564_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6651405.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            29981..30694
                     /gene="purC"
                     /locus_tag="OO564_000135"
     CDS             29981..30694
                     /gene="purC"
                     /locus_tag="OO564_000135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6651406.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            30862..31725
                     /locus_tag="OO564_000136"
     CDS             30862..31725
                     /locus_tag="OO564_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6651407.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            31740..33755
                     /gene="tmcA"
                     /locus_tag="OO564_000137"
     CDS             31740..33755
                     /gene="tmcA"
                     /locus_tag="OO564_000137"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6651408.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            33829..34527
                     /gene="ypfH"
                     /locus_tag="OO564_000138"
     CDS             33829..34527
                     /gene="ypfH"
                     /locus_tag="OO564_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6651409.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(34637..34837)
                     /locus_tag="OO564_000139"
     CDS             complement(34637..34837)
                     /locus_tag="OO564_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6651410.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(34865..35992)
                     /gene="dapE"
                     /locus_tag="OO564_000140"
     CDS             complement(34865..35992)
                     /gene="dapE"
                     /locus_tag="OO564_000140"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6651411.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            complement(35996..36352)
                     /locus_tag="OO564_000141"
     CDS             complement(35996..36352)
                     /locus_tag="OO564_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6651412.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            36733..36792
                     /gene="ypfM"
                     /locus_tag="OO564_000142"
     CDS             36733..36792
                     /gene="ypfM"
                     /locus_tag="OO564_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6651413.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(36891..40004)
                     /gene="acrD"
                     /locus_tag="OO564_000143"
     CDS             complement(36891..40004)
                     /gene="acrD"
                     /locus_tag="OO564_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6651414.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(40206..41929)
                     /gene="narQ"
                     /locus_tag="OO564_000144"
                     /pseudo
     CDS             complement(40206..41929)
                     /gene="narQ"
                     /locus_tag="OO564_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            42135..44114
                     /gene="aegA"
                     /locus_tag="OO564_000145"
     CDS             42135..44114
                     /gene="aegA"
                     /locus_tag="OO564_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6651415.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            44182..44757
                     /gene="nudK"
                     /locus_tag="OO564_000146"
     CDS             44182..44757
                     /gene="nudK"
                     /locus_tag="OO564_000146"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6651416.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            44883..45914
                     /locus_tag="OO564_000147"
     CDS             44883..45914
                     /locus_tag="OO564_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6651417.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(46028..48031)
                     /gene="tkt"
                     /locus_tag="OO564_000148"
     CDS             complement(46028..48031)
                     /gene="tkt"
                     /locus_tag="OO564_000148"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6651418.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(48051..49001)
                     /gene="tal"
                     /locus_tag="OO564_000149"
     CDS             complement(48051..49001)
                     /gene="tal"
                     /locus_tag="OO564_000149"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6651419.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49290..51569
                     /gene="maeB"
                     /locus_tag="OO564_000150"
     CDS             49290..51569
                     /gene="maeB"
                     /locus_tag="OO564_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6651420.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            51862..52197
                     /gene="eutS"
                     /locus_tag="OO564_000151"
     CDS             51862..52197
                     /gene="eutS"
                     /locus_tag="OO564_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6651421.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            52210..52689
                     /gene="eutP"
                     /locus_tag="OO564_000152"
     CDS             52210..52689
                     /gene="eutP"
                     /locus_tag="OO564_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6651422.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            52664..54037
                     /gene="eutA"
                     /locus_tag="OO564_000153"
     CDS             52664..54037
                     /gene="eutA"
                     /locus_tag="OO564_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6651423.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            54561..55252
                     /locus_tag="OO564_000154"
     CDS             join(54561..54807,54807..55252)
                     /locus_tag="OO564_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6651424.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(55371..56180)
                     /gene="murI"
                     /locus_tag="OO564_000155"
     CDS             complement(55371..56180)
                     /gene="murI"
                     /locus_tag="OO564_000155"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6651425.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(56214..57674)
                     /locus_tag="OO564_000156"
     CDS             complement(56214..57674)
                     /locus_tag="OO564_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6651426.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(57667..57945)
                     /locus_tag="OO564_000157"
     CDS             complement(57667..57945)
                     /locus_tag="OO564_000157"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651427.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            complement(57926..58411)
                     /locus_tag="OO564_000158"
     CDS             complement(57926..58411)
                     /locus_tag="OO564_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6651428.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            complement(58723..58929)
                     /locus_tag="OO564_000159"
     CDS             complement(58723..58929)
                     /locus_tag="OO564_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651429.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            complement(59394..59936)
                     /locus_tag="OO564_000160"
     CDS             complement(59394..59936)
                     /locus_tag="OO564_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651430.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            complement(60001..60222)
                     /locus_tag="OO564_000161"
     CDS             complement(60001..60222)
                     /locus_tag="OO564_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651431.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            complement(60240..60635)
                     /locus_tag="OO564_000162"
     CDS             complement(60240..60635)
                     /locus_tag="OO564_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6651432.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            complement(61395..62147)
                     /locus_tag="OO564_000163"
     CDS             complement(61395..62147)
                     /locus_tag="OO564_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651433.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            complement(63198..63962)
                     /locus_tag="OO564_000164"
     CDS             complement(63198..63962)
                     /locus_tag="OO564_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6651434.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAE
                     EDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLY
                     TWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGK
                     CGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            complement(64446..65651)
                     /locus_tag="OO564_000165"
     CDS             complement(64446..65651)
                     /locus_tag="OO564_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6651435.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            65840..67201
                     /gene="eutB"
                     /locus_tag="OO564_000166"
     CDS             65840..67201
                     /gene="eutB"
                     /locus_tag="OO564_000166"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR6651436.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            67222..68109
                     /gene="eutC"
                     /locus_tag="OO564_000167"
     CDS             67222..68109
                     /gene="eutC"
                     /locus_tag="OO564_000167"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6651437.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            68119..68778
                     /gene="eutL"
                     /locus_tag="OO564_000168"
     CDS             68119..68778
                     /gene="eutL"
                     /locus_tag="OO564_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6651438.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            68791..69252
                     /gene="eutK"
                     /locus_tag="OO564_000169"
     CDS             68791..69252
                     /gene="eutK"
                     /locus_tag="OO564_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6651439.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            69337..70389
                     /gene="eutR"
                     /locus_tag="OO564_000170"
     CDS             69337..70389
                     /gene="eutR"
                     /locus_tag="OO564_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6651440.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(70395..71294)
                     /gene="hemF"
                     /locus_tag="OO564_000171"
     CDS             complement(70395..71294)
                     /gene="hemF"
                     /locus_tag="OO564_000171"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6651441.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71298..72167)
                     /gene="amiA"
                     /locus_tag="OO564_000172"
     CDS             complement(71298..72167)
                     /gene="amiA"
                     /locus_tag="OO564_000172"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6651442.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            72381..72806
                     /locus_tag="OO564_000173"
     CDS             72381..72806
                     /locus_tag="OO564_000173"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6651443.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            72793..73242
                     /locus_tag="OO564_000174"
     CDS             72793..73242
                     /locus_tag="OO564_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6651444.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            73303..73878
                     /locus_tag="OO564_000175"
     CDS             73303..73878
                     /locus_tag="OO564_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6651445.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            73974..74873
                     /gene="yfeX"
                     /locus_tag="OO564_000176"
     CDS             73974..74873
                     /gene="yfeX"
                     /locus_tag="OO564_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6651446.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(75053..76477)
                     /gene="murP"
                     /locus_tag="OO564_000177"
     CDS             complement(75053..76477)
                     /gene="murP"
                     /locus_tag="OO564_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6651447.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(76481..77377)
                     /gene="murQ"
                     /locus_tag="OO564_000178"
     CDS             complement(76481..77377)
                     /gene="murQ"
                     /locus_tag="OO564_000178"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6651448.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            77541..78341
                     /gene="murR"
                     /locus_tag="OO564_000179"
     CDS             77541..78341
                     /gene="murR"
                     /locus_tag="OO564_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6651449.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            78528..79319
                     /gene="ucpA"
                     /locus_tag="OO564_000180"
     CDS             78528..79319
                     /gene="ucpA"
                     /locus_tag="OO564_000180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6651450.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            79623..80639
                     /gene="cysP"
                     /locus_tag="OO564_000181"
     CDS             79623..80639
                     /gene="cysP"
                     /locus_tag="OO564_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6651451.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            80639..81472
                     /gene="cysT"
                     /locus_tag="OO564_000182"
     CDS             80639..81472
                     /gene="cysT"
                     /locus_tag="OO564_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6651452.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            81472..82347
                     /gene="cysW"
                     /locus_tag="OO564_000183"
     CDS             81472..82347
                     /gene="cysW"
                     /locus_tag="OO564_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6651453.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            82337..83434
                     /gene="cysA"
                     /locus_tag="OO564_000184"
     CDS             82337..83434
                     /gene="cysA"
                     /locus_tag="OO564_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6651454.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            83568..84479
                     /gene="cysM"
                     /locus_tag="OO564_000185"
     CDS             83568..84479
                     /gene="cysM"
                     /locus_tag="OO564_000185"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6651455.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(84683..85406)
                     /locus_tag="OO564_000186"
                     /pseudo
     CDS             complement(84683..85406)
                     /locus_tag="OO564_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(85439..85813)
                     /locus_tag="OO564_000187"
     CDS             complement(85439..85813)
                     /locus_tag="OO564_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6651456.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            85918..86769
                     /gene="pdxK"
                     /locus_tag="OO564_000188"
                     /gene_synonym="thiJ"
     CDS             85918..86769
                     /gene="pdxK"
                     /locus_tag="OO564_000188"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6651457.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(86811..87320)
                     /gene="crr"
                     /locus_tag="OO564_000189"
     CDS             complement(86811..87320)
                     /gene="crr"
                     /locus_tag="OO564_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6651458.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(87361..89088)
                     /gene="ptsI"
                     /locus_tag="OO564_000190"
     CDS             complement(87361..89088)
                     /gene="ptsI"
                     /locus_tag="OO564_000190"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6651459.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(89133..89390)
                     /gene="ptsH"
                     /locus_tag="OO564_000191"
     CDS             complement(89133..89390)
                     /gene="ptsH"
                     /locus_tag="OO564_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6651460.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(89774..90745)
                     /gene="cysK"
                     /locus_tag="OO564_000192"
     CDS             complement(89774..90745)
                     /gene="cysK"
                     /locus_tag="OO564_000192"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6651461.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(90930..91691)
                     /gene="cysZ"
                     /locus_tag="OO564_000193"
     CDS             complement(90930..91691)
                     /gene="cysZ"
                     /locus_tag="OO564_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6651462.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            91921..92808
                     /gene="zipA"
                     /locus_tag="OO564_000194"
     CDS             91921..92808
                     /gene="zipA"
                     /locus_tag="OO564_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6651463.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            92879..94894
                     /gene="ligA"
                     /locus_tag="OO564_000195"
     CDS             92879..94894
                     /gene="ligA"
                     /locus_tag="OO564_000195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6651464.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            94896..95114
                     /locus_tag="OO564_000196"
     CDS             94896..95114
                     /locus_tag="OO564_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6651465.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(95111..96109)
                     /locus_tag="OO564_000197"
     CDS             complement(95111..96109)
                     /locus_tag="OO564_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6651466.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            96199..97125
                     /locus_tag="OO564_000198"
     CDS             96199..97125
                     /locus_tag="OO564_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6651467.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(97116..97457)
                     /locus_tag="OO564_000199"
     CDS             complement(97116..97457)
                     /locus_tag="OO564_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6651468.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(97577..97699)
                     /locus_tag="OO564_000200"
     ncRNA           complement(97577..97699)
                     /ncRNA_class="other"
                     /locus_tag="OO564_000200"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT        23781 a        24656 c        25552 g        23711 t
ORIGIN      
        1 aatacggtcg ccgaactttc catcgtgacc aacgccccgg aaaatatgca gcgttgcggg
       61 gcaatgtatt tgcaacgcga attgaaagat aaagtcgcga tgatgaatcg actacagcag
      121 gcgctggagc acaaccattt tttcctgatg gcccagccga ttaccggtat gcgtggtgat
      181 gtttaccatg aaattcttct gcgcatgaaa ggtgagaatg atgaactgat cggccccgat
      241 agcttcttgc cggtcgcgca cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt
      301 gagcatacgc tgcaatttat ggctgaaaac agagcgaaga tgcccgctca ccgttttgct
      361 attaatctgt ctccaacctc ggtatgtcag gctcgttttc ctgttgaagt cagtcagttg
      421 ctggctaaat atcagattga agcgtggcaa cttatttttg aagtcaccga aagtaatgct
      481 ctgaccaatg ttaagcaggc gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag
      541 attgcgattg atgatttcgg caccggctac gccagctatg cacggcttaa aaatgtgaat
      601 gccgatctgc ttaaaattga cggcagcttt atccgcaata ttgtgtcaaa tagtctggat
      661 tatcagattg tggcgtcgat ttgccatctg gcgcgaatga agaaaatgcg ggtagtggca
      721 gagtacgttg aaaacgaaga gatccgcgag gcggtgctct ctttggggat cgattatatg
      781 cagggttatc ttattggtaa gccgcaaccg ttaattgata cgctgaatga aatcgaaccc
      841 attcgcgaaa gtgcctgaat aatgcgggcc gacatttctc gtcggcccgc aaagcattaa
      901 gcggcgattt ctggggtact ttcttcttca attttcaacc gccagccagc cacgccttcc
      961 cagtattctt gctccttttc cagatcaagc agtaccagcg cattctgact aaaccagtca
     1021 tgcgggaaac gcagtgtcca gtgactgtca tcagtaatca gtgtcaatgt tgacggtgtg
     1081 gtggttgcct gacgttgatt gtttagtaat acgccaaggc gcaatagctg tatcagtggc
     1141 aggaactgtt tcttcttaaa caaggtaaag cgcggcagat cgtcgagctt aatcgcttta
     1201 cggtgatagc gcagcagtgt cgccatcatc agctgctgtt cctgattaaa acccggcaag
     1261 tcactgtttt gcagaatata agcggagtgg cgatgcaaac cgctgtggtt gatattcaac
     1321 ccgacctcat gcagcatggc ggcccatcgc agtagcgcct ccagttgcgg atgcgccagc
     1381 ttcggttgct gttcccgcca ctgttcgtac atttgcatag tggtatccag cactcgtcgg
     1441 gcctgttcgc tgtctatgtg atactggttg gcgaggctgc tggcggtgcg actacgcaca
     1501 tcctgatgac ggaaacgtcc ttccatttca tacagtacgc cttcgcgtaa cgccccgtca
     1561 gaaaggcgca gttcacggat ggctaaagca tcaaacacac cacataaaat cgccagtcca
     1621 ggaacgaaga ctgttttccg ctcttcggaa agacccggta aactcagcga tgcgaaatta
     1681 cggtgacgta aaatttcttt taccagtttt tccagacgtt ccggggtaat tatcccgtct
     1741 ttctcgccca tttccattaa cacttcatgg gcggctttta tggtaccgga agcgcccatt
     1801 gcaacgttcc agccctgaat acggaattgc caggttaaag tttccagttt ttgtgctgcc
     1861 gccatgcgag cgcgctgaaa attctcttta ttgatgaccc cgccaggaaa ataaagctgg
     1921 gcaaagctga cacaacccat ccggcggctt tcaacgagaa taggttcgaa attttcacca
     1981 atcaccagtt ccgtagatcc gccgccaata tcaataacca gtttgcgacc tttttccggt
     2041 tgggtatgtt ccacgcccat aaaaatcaga cgggcttctt cattaccgga aataatttca
     2101 atcgggtagg gaatgacctt ttccgcgcgt ttcagaaagt cagtggcgtt cagcgcctga
     2161 cgcagcgtat gggtgccgac tatacagacg ctggcaggag aaaacccttg tagccgttcg
     2221 gcaaacagcg acagacagtt caaaccgcgc gtcattgcct cttcactcaa catattatct
     2281 ggccccaggc cgtccgccag atgcacccgc tgtttcaggc ggccaataat ctgcatggca
     2341 ccatctacca cacgggctat gaccatgtga aaactgtttg aaccaagatc gaccgcagca
     2401 aactcctgcg gacgagggga tttatcgtgt attggcatag ggttattcag gttgttcgag
     2461 tgatttgatg tagtcgtaaa tcgccaactg cgcccgtact ttgcggcgat tgccgcgggg
     2521 aacgtagcga ttactgagtt ctttatcgat ataacgtgct ttgaccgtat cgctgaacaa
     2581 tatgtcgatg atgtccagca cccgctgctt caggcgcgga tcgagcagcg gtgtcgccac
     2641 ttcaatacga taatcaatat tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt
     2701 atcgccgcca ttttcaaaaa tataaacccg gtcatgttca aggtaacggt caacaatact
     2761 gatggcacga atgttgtcgc taatgccttc cagattgggg atcagcgaac acattccgcg
     2821 aaccagcaga ttaaccggta cgccggagct ggaggccgca tacagacgat caaccaggcc
     2881 tttatcgaca aggttattta gcttcagggt gataccactg ggcagccctt gctgcgcgtt
     2941 ggcgatttcg cggtctacca tttcatataa cagacggcga gagttttgcg gtgacaccat
     3001 taaataatca aatgtcaccg ggcggtatgg gttttcaata aagttaaata cccgccgtac
     3061 ttcgttggtg atacgcgcat ctgcggtcag caacgaatag tcagtataaa gacgcgcggt
     3121 tttttcgtta aagttcccgg tcccgatgtg tgcgtaacgg accacttcac cgttttcttt
     3181 acgtgaaatc aggaacagtt tggcgtgaat tttcagcccc ggcgcagaga agataacgtg
     3241 cacgcctgct tcggtcaggc gcttcgccca gtgaatgttg gcttcttcgt cgaaacgcgc
     3301 ctgtaactca accaccacag tgactttctt accgttatgt gcggcgtgga tcatcgagtc
     3361 gatgatgcgt gaatctttcg ccacgcggta aatgttaatt ttaatcgcca gtacgctcgg
     3421 gtcgaacgaa gcctgacgca gcagttccag cacatgccca aaggtgtgat aaggataata
     3481 gagcaacaca tcgcgttcgc gaatggcatc aaaaccattg cggaactggg ctttatcaaa
     3541 ccaaatatgg cgtaaacgcg gcagtggttt gttcaccaga ttggctttgc cgacattggg
     3601 aaaattaata aagtctttaa aattatgata acgaccgccg gggacgatgg agtcgtagcg
     3661 ggaaatagtc agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata
     3721 aacaaaacgc accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat
     3781 caggctggct tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga
     3841 ataggcattc agcgcgtcat aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg
     3901 cagaatgtta tccagaagaa tcatcggctt gcgtcgacgc ggcgcttctg gcggtaaatt
     3961 cacaaagcgc ggcactttat ctgatgggat ctccagcagc gcgtaacgga tgctatcgct
     4021 acggataatt tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt
     4081 gtcaggattg attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg
     4141 cagccagttt tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg
     4201 cgccatctcc agcaatagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac
     4261 ccgggactga attttgccca gtaaatggcg ggaatgagcg ttggagcctt gttcttcgct
     4321 aataatgatg cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt
     4381 ggaatagatc cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc
     4441 ctgaagcacg cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc
     4501 ctgacccatt accactttta ctccgtttta ttcacgggac actgcctgga tattatggcg
     4561 agcaatattg gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg
     4621 cgatttacag gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacgggggca
     4681 ttactcgtcg gcagcgtagc tctgcggcgg cagacgttga ccatccagcc acgcggcgtt
     4741 ttcgtgcatt ttcagacgac catcggcaaa ccagctaatc accagtggat aaatagcgtg
     4801 ttcctgggtt tgcacgcggg cggtgatgtc atcttccgta tcaccagcaa ataccgggac
     4861 tttcgcctgt aaaataaccg ggccaccgtc cagttcatcg gtgacgaaat gcaccgatgt
     4921 accgtgctct tcatcgccat tttccagcgc ctgacgatgg gtgtgtaatc cgggatattt
     4981 cggcagcaga gaagggtgaa tgttcagcaa acgcccggca tagtgggaga caaacgccgg
     5041 gctgagaatg cgcataaaac cagccagcac gaccacatcg ggtgcgtaca tgtcgatttc
     5101 atgaatcaac tcccggtcat aggcttcacg actgtcaaac gcgctggcga tgagcgtatg
     5161 cgttgcaata cccgcctggc gggcgcgttc aaggccgaac gcgtcggcct tattgctgaa
     5221 aactgcccgt acggtgcctt taattttgtt ggttttacag gcgtcaataa ttgcctgtaa
     5281 attacttccg ttgccggaaa taagcaccac aatattcatt attcgataac cacgcgttgt
     5341 tcggaatcag aggctttgat gataccgatt ttccacgcgt tttcaccgtt ggcatttagc
     5401 agggcgaggg ctttgtccac ttccggagca ggcagggcga taatcatccc gacgccgcag
     5461 ttgaaggtgc gatacatttc atggcgctca acgttacctg ccgtttgcag ccagttgaac
     5521 acttccggcc actgccagga agattcatca atcactgcct gggtattatc tggcaatacg
     5581 cgcggaatgt tttcccagaa gccgccgccg gtcaggtgcg caatggcatg cacatcgacc
     5641 ttttcaatca actccagcac tgacttcacg taaatgcggg tcggtgccag cagatgatcg
     5701 gctaatggct taccatcaag ttcggtggtt tgcggatcac aaccgctgac ttcaagaatt
     5761 ttgcgcacca gcgaatagcc gttcgagtgc ggaccgctgg aaccgagtgc aatcagcaca
     5821 tcgccgtcgc tgactttaga gccgtcgatg atttctgatt tttctaccac gcccacgcag
     5881 aaacccgcga catcgtaatc ttcaccgtga tacatccccg gcatttctgc cgtttcgcca
     5941 cccaccagag aacagcccga ttgcagacaa ccttccgcga tgccgctgat cactgctgaa
     6001 gcggtatcaa catccagttt tccggttgcg taatagtcga ggaaaaacag cggttccgca
     6061 ccttgcacca ccaggtcatt aacgcacatg gcgaccagat caataccaat ggtgtcgtga
     6121 cgttttaagt ccattgccag acgcagcttg gtacctacgc cgtcagtgcc ggaaaccagc
     6181 acgggttcac gatatttttg cggcaatgca cacagcgcac cgaagccgcc cagaccgccc
     6241 atcacttccg gacgacgcgt tttcttcact acgcctttga ttcttccaac cagagcatta
     6301 cccgcgtcaa tatcaacacc ggcatctttg tagctaagag aggttttatc ggtcactgct
     6361 tgggtcccca cgcgttactt gcggtagaaa aataaaattc ggcgcaattc taacagggaa
     6421 agcaaacgtt tgcgagactg ttttacacaa cctttttgca cgtcttttcc ccgggcgcgc
     6481 ggcgaaagaa gacttgtgcc agggcaaagg ttagttttcg gatggaataa tcttctttca
     6541 taaccatttg aatataaaat aactttatct caaactgtta tcattttgac taaagtcaac
     6601 gaaaataata ttgccgcctt gaagaaagga ggtataatcc ttcgattttt tttgtggctg
     6661 cccctcaaag gagaaagagt atgaagatcg tggaagtcaa acacccactc gtcaaacaca
     6721 agctgggact gatgcgtgag caagatatca gcaccaagcg ctttcgcgaa ctcgcttccg
     6781 aagtgggtag cctgctgact tacgaagcga ccgccgacct cgaaacggaa aaagtaacta
     6841 tcgaaggctg gaacggcccg gtagaaatcg accagatcaa aggtaagaaa attaccgttg
     6901 tgccaattct gcgtgcgggt cttggtatga tggacggtgt gctggaaaac gttccgagcg
     6961 cgcgcatcag cgttgtcggt atgtaccgta atgaagaaac gctggagccg gtaccgtact
     7021 tccagaaact ggtttctaac atcgatgagc gtatggcgct gatcgttgac ccaatgctgg
     7081 caaccggtgg ttccgttatc gcgaccatcg acctgctgaa aaaagcgggc tgcagcagca
     7141 tcaaagttct ggtgctggtc gctgcgccag aaggtatcgc tgcgctggaa aaagcgcacc
     7201 cggacgtcga actgtatact gcatcgattg accagggact gaacgagcac ggatacatta
     7261 ttccgggcct cggcgatgcc ggtgacaaaa tctttggtac gaaataaaga ataaaaataa
     7321 ttaaagccga ctttaagagt cggctttttt ttgagtaaag cgcctataac acataataca
     7381 gaggataata ctatgacgcg ccgtgctatc ggggtgagtg aaagaccgcc acttttacag
     7441 acaatcccgc ttagtttgca acatttgttc gccatgtttg gtgcaaccgt cctggtgccc
     7501 gtcttatttc atattaaccc ggcgactgta ctgttattta acggtattgg aacgctgctg
     7561 tatctcttca tctgtaaagg gaaaattccg gcttatcttg gttccagctt tgcctttatt
     7621 tcaccggtat tgttactgtt gccgttaggg tatgaagtcg cgctgggcgg ctttattatg
     7681 tgcggcgtgc tgttctgcct ggtttctttt atcgtgaaga aagcggggac cggctggttg
     7741 gacgtgctgt ttccacctgc ggcaatgggc gcaatcgttg ccgtcatcgg tctggagctg
     7801 gcgggcgtag ctgccggtat ggcgggttta ctcccggctg aagggcaaac gccaaactcc
     7861 aaaaccatca tcatctctat taccaccctg gcggtcacgg ttttaggttc cgtgctgttt
     7921 cgtggtttcc tggcaattat cccgatttta attggcgtgc tggtggggta cgcgctctct
     7981 ttcgcaatgg gaattgtcga taccacgccg attattaatg ctcactggtt tgcactgcca
     8041 accctctata cgccgcgctt cgagtggttt gccattctga ctattctgcc agcggcgtta
     8101 gtggttattg ccgaacacgt agggcacctg gtagtaacgg ctaatatcgt caaaaaagat
     8161 ctgctgcgcg atccaggtct gcaccgttcg atgtttgcta acggcctgtc gaccgtgatc
     8221 tccggcttct ttggctctac gccaaatacc acttacggag aaaacattgg cgtgatggcg
     8281 atcacccgtg tttacagtac ctgggttatc ggcggggcgg cgattttcgc tatcctgctt
     8341 tcctgcgtcg gtaaactggc tgccgctatc cagatgatcc cattgccggt gatgggcggc
     8401 gtttcgctgc tgctttatgg tgtcattggt gcttccggta ttcgtgtttt gatcgaatcg
     8461 aaagtggatt acaacaaagc acagaacctg atcctgactt ccgtgatttt gatcatcggc
     8521 gtcagtggcg cgaaggtaaa catcggcgcg gcggagttaa aaggtatggc gctggcgacc
     8581 atcgtcggta tcggcttaag tttgattttc aaactgatca gtgtgctgcg ccctgaagaa
     8641 gtggtgctgg acgcagaaga cgcggatata acagacaaat aatgtaccga ccgggcagtg
     8701 ttgctgcccg gttcaaacat catgggattc tgtggtaaac ttctcgcgat tttgtgaaat
     8761 cctggttgag gtatctctga acacaccggc acagctctct ttgccacttt atcttcctga
     8821 cgacgaaacc tttgcaagtt tctggccggg ggataactcc tctttactgg ccgcgctgca
     8881 aaacgtgctg cgtcaggaac atagcggtta catctatctc tgggcacgcg aaggcgcggg
     8941 gcgcagccat ctgctgcacg cggcttgcgc ggaattgtcg cagcgtggcg atgcggtggg
     9001 ctatgtcccg ctggataaac gcacctggtt tgttccggaa gtgctcgacg gtatggagca
     9061 tttgtcgctg gtctgtatcg acaacattga gtgtattgca ggcgatgagt tgtgggagat
     9121 ggcgattttc gatctctaca atcgaattct ggaatcgggc aaaacacggt tgttgatcac
     9181 cggcgatcgt ccaccgcggc agttgaatct gggattaccg gatctcgcgt cgcgtctcga
     9241 ctgggggcag atctacaaat tgcagccact ttctgatgaa gataagttgc aggcgctaca
     9301 gttacgcgcg cgtttgcgtg gttttgaact gccggaagat gtggggcgtt tcttgctgaa
     9361 gcggctcgac agagaaatgc gcacgctatt tatgacgttg gatcagttgg atcgtgcgtc
     9421 gattaccgcg caacgtaagc tgaccattcc gtttgtgaaa gaaattctga agttgtagat
     9481 aaggtgttta ttgtcggatg cggcgcgaac gcctgaccta cgcgaagtta atccatagta
     9541 ttcgtaggcc ggataagacg tggcaagcgt cgcatccggc aatgtgcttc aactagcgat
     9601 ggagcacatc ttaacccacg atttccagta cctgctccgg tggacgacca atccgcgctt
     9661 tgccattcgc gaccataatc gggcgctcca tcagcttcgg attatccacc attgcctgaa
     9721 tcagcgcctc ttcgctaagc gaactgtcag ccaggtttag ttctttataa agatcctctt
     9781 tctggcgcat caattcacgg gcgctattca tccccagcat tttcagcaaa tcgcgcagcg
     9841 ttgccgcatc ggcgggtgtc tcaaggtaga gcaccacttc tggctccacg ccgttttctt
     9901 tcagcaggtt cagcgtttcc cggctctttg aacagcgtgg gttatggtag atttttacct
     9961 gtttggtcat tgttcttcct ttaatgcgaa ttacatcttg gtataaggct taaagcgttc
    10021 ctgcagctgg cgcaactggt cgatgcgcgc atcgtaacgc gcttgttgca ggctgcctaa
    10081 tttcacctgc gaactggcgc tactcaacag cgaaatggcc tgatcgagtc gtccggcgag
    10141 cgcataacct tctgctcgcg cagccagctc ctgatcgcgg ttatttagcg cggcttccgc
    10201 ctgtgccagc aaatcccagc cgttgctgtc atctttatta ttaaaggtgt agcgattcag
    10261 aatattggcc gcttcttgtg gttgaccgcc ttgcagataa gcgttcgcca ggttgagctg
    10321 caacaccgga ttggtgcgta aatcgcgggc atttttcagg cgattgattg cctcattggc
    10381 tttgttttgc tcaagatcga tatcggtagc cagatcgaga taccatgcgt tgccaggttc
    10441 tgccgccagt aacggttgca gcgttttacg cgcctcgtcg tatttattgg ctttcatcgc
    10501 ctgtaaagca cgaccatatt gcgccgctcg ttgctgaaga acgtttcctt tcgcccattc
    10561 atccagcaaa ttactggtga gctggttacg tccggaatta tacatcccca gtgtgcgcgc
    10621 tttcgccaga tagaaatctt ccgacgactg caccaccatc gggcgcatct gattagcacg
    10681 gttgcgggca tctgccagac gactttccgg caacgggtga gtcaataaaa tttccggcgg
    10741 gcgcgaggag taacgcgcct gatcgagcaa tttttccagg aaggtcggca tcgcctgtgg
    10801 atcgaatccc gagcgttgca gcacctgaat accaatgcgg tccgcttcct gttcattttg
    10861 ctgggtgaaa ctgatcatcc cctggcgcgt tcccgccagt gtaccggtca gcgccgccat
    10921 ccccgcctgc ggactggcca tcgccagtaa aatagaacct aacgcgccga cccaggtcag
    10981 cggcgcgctg cgttgttgat cttccatcgc tcgcgccagg tgacgttggg tgacgtggga
    11041 gatttcgtgc gccataactg aagccagttg actttcgtta tcggaataac ggaacagggc
    11101 agagtgcagc accacgttgc cgccaaagaa agcaaaggcg ttaatttcgt cgttgttgat
    11161 cagaaaaaaa tgaaacggtg tcttaaccga attggcatgc gaaaccagac gcatccccag
    11221 cgaattaata tattgcgtta acagcgggtc attaattaac ggcgcgctgc cgcgtagctg
    11281 gcggacataa tagtcgccca tctgcatttc ctgaccaatg gaaagcgtgc ttcctgcgga
    11341 ggttcccata tccggcaagg tgtctgcgct gtcggcaaac gccggggcta cctgaccaat
    11401 ggtcatagca gcaatgaggg ttgcaaccag gttttttttc aactgcctga acataacctc
    11461 tatcctgtat ttctgaagat agtcatttga ccgatactgt gaaatatcgt tcccacctcg
    11521 ccgcctttga gtgtaacggc gaagattagc ccactcaaat gacttttcag tttcctgaat
    11581 ccgtcataaa aagcgaagtc ttttttgtga catttcgata caattgcgga tcgcaatccc
    11641 gctcttgagg ttcagggaag gtctttatgc tcgaaatgtt gatgcaatgg tatcgccgcc
    11701 gttttagcga cccggaagcg attgccttgc tggttatttt agttgccgga tttggcatta
    11761 tctttttctt tagtggcctg cttgctccgt tgctggtggc tattgtgctg gcttatttgc
    11821 tggaatggcc aaccgtgcgc ctgcaatcta ttggctgctc ccgccgctgg gcgacgtcga
    11881 ttgtattggt ggtttttgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg
    11941 cctggcaaca gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg
    12001 actttgccgc cacgttgccg cgccgctatc cggtgttaat ggacgcgggc attattgatg
    12061 caatggccga aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt
    12121 ccctcgcctc gctggtcggt ttgctgacca tagccgtcta tctggtgctg gtgccattga
    12181 tggtcttctt cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc
    12241 cgcgtaaccg tgggctggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact
    12301 atatccgcgg caaagtgctg gagatgatcg tggtggggat cgccacctgg ctggggttct
    12361 tgctcttcgg gctgaactat tcgctgctgc tggcagtgct ggtcggcttc tcggttctta
    12421 ttccgtacat tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt
    12481 ttggcgcagg tactgaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg
    12541 acggcaacct gctagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga
    12601 ttattttatc ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca
    12661 ttccattggc gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatcgcgc
    12721 aagaataatc ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg
    12781 taaataaata gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag
    12841 catcaccggg agcagattgg cggtaataaa atggctgacc gtcagtgccg gaaaattgtc
    12901 tgcgctactg tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc
    12961 gaacggaatc acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt
    13021 gatggacaaa atcatggcga cgattttgtc gcataacgaa cgtccgcaat aactcatcca
    13081 cagcgccagg caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt
    13141 atgatgcatt ttgccctcgg cgcagtgtaa aatcgcgact ccccacatcg cgttttcact
    13201 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
    13261 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
    13321 tgccattacc gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat
    13381 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctatg aagacggggc
    13441 atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
    13501 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
    13561 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
    13621 tcctgcaaaa gcagattaca acacctcgcg aaccgagatt cccagacgct gcattcgtga
    13681 cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat
    13741 gccattggtt tctcgcaata cctgaacaat gcgctgacgt tcttcttcgt cattctctgg
    13801 cgtcgtcggg tgcagcaact gcgccattga actgcgaagc gctgaatctt catttagcgt
    13861 cggcagtaaa cggctttgtc ggacattaag atgtaaattc agactgttac cgcgagtcag
    13921 cagtaccgct cgctcaatca cgttttccag ctcgcgcacg ttgcccggcc agtcccacga
    13981 catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt gaacgattca tatggcgcgc
    14041 cattttttgc gtgaaatgct ttgctaaaag agggatatct tccggacggt cgcgcagcgg
    14101 aggcaattcc agtgggaaga cattcaggcg ataaaagaga tcgctgcgaa actggcgatc
    14161 ttcaaccatt tgccacaaat cacggttggt ggcggcaatg actctgacat ttaccgggat
    14221 cgttctactc ccgccgagac gctcaatctc ccgttcctgc aatacgcgca gcagtttagg
    14281 ctgaagttct aacggcagat cgccaatttc atcgagaaac aacgtgccgc catcggcaat
    14341 ttcaaaacga ccacgatggg tattaatcgc accagtaaac gcccctttgt catgaccgaa
    14401 taactcactt tccagaagac tggcggggat ggcagcgcag ttgattttga ccagcggctt
    14461 gtcgtgtcgc gggctaagtt gatggatcgc tctggcgatc acctctttgc cggttccggt
    14521 ttcaccgcaa atcaacaccg tactgtcgct cttcgccaca atatctacct gctggagcag
    14581 gtcttccatt gcctggcttt gatagataat gtcaccgacg cccagattcg aaaggagctg
    14641 ctcgctaagc tggtggtttt cttgctgcaa actttcctgc aaatcggtca ttctacggca
    14701 ggcatcggca ttgccaacgg caatagcgat gcgatcggct atgtgttgta gtagctggca
    14761 gttttcctca ctaaagagag tggaagaggt atgcgccagc aacaatgcac ccggtgtatg
    14821 gttgccaaag gtaagcggta taaggagcgc agattcgcag ccgttagatg caagtaagag
    14881 taataacggg tcgcgttgcc agagaaacag atcatctgca cggtgcgtca aggtcggttg
    14941 tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca ggcatattgt gtggcagaca
    15001 cgcacaatgt gaggcagaaa gatcgctgca ccacaggctg aacttctcgt tctttcgatg
    15061 atcgcccagt accatactga ctgaagccag accgaaaaaa tgatggatct cacgagcgac
    15121 gtcagcgatc agatcgtcga gatcaagatg tgacagcact gcattagtga tatccaccag
    15181 tacccgatga tgatcgcgat ctttctccag caaatgcagc gccacctgct gttcttcatc
    15241 attgagccat gattgcagaa ccggggtgat aatgcgtgcc agccagttaa acagtgtgca
    15301 atcctgggga gtaaaacggc tgccgtgggt acgaataaag cgtagctccg ccattcaact
    15361 actgccgaga aggacggcgt gacgcgaata atcgaccatc tcgccctgat actctgttgt
    15421 tgccgtcaca tcgctgccat catcatggta gcggtaatag aagttgcttt tgagaacatg
    15481 cagttcaata agttgtaccg ggctgaacgg cggcggcagc gggatgaagg cgcgtaataa
    15541 agacgccttg ccgtgtttct gcgctaaccg ctccgcgagc attccgttta ccgcttcaat
    15601 tgttattcct tgcggcgggg caaacatcgc ttcgtctgac atagccatca acagtcctta
    15661 ttaatgagaa atccttctca cgcaattttt ctggccatca gatagatagc cggttccagc
    15721 acaatttcat ccaacagatg aatgaattga atgctacgcg cttgccacac agacgtcgcg
    15781 tcatctgcca gtggcgcgaa tgcccgggtc aacagggcgg tgtggctggc gtcaataaca
    15841 atctcaccaa aagtcatcac ccccagcatc ttacgtcggg cttgctcctc ttcgaccagt
    15901 gcaagccaat cttcgtattc cgtcagtggg cagcggaagg cgacattcag acaatcaatc
    15961 acgccaacgt gatgaccgat agccagcgag taatacatta cctgagagct gtgttccggc
    16021 atctcgtcgc tactggcgac aaactttttt cgcagcgtcc agaaaacaat ttccccgcac
    16081 tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc tgaacgatac tattgagacg
    16141 tggatcgtcg gcatcgcgca accaggtgtt aacccgattt cctgtaggat cgtcgcttaa
    16201 atggcgcagg agccgatcgc aaatttctcg cccctgacga tagcccgcca gccgccgcgc
    16261 ttctcgctca atggcgatac gctgtgatgg cgggatctgc ggccacaacg gttgcatagt
    16321 caccccggtg ggatcgcgat aatccaccgc gtgaatcttc tgttgcagca aaccgagcgc
    16381 cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg cagccgggga tccaaacatc
    16441 aatggggaca atggtgtcgc tgccgcccca gacgctgtag agatcgtgga aaataccgcc
    16501 gccgacaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc
    16561 ccgaagtgca ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc
    16621 gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata cgggggtgat
    16681 ggcagcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgatgc gataaacgta
    16741 agccgagcga cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag
    16801 cgtgatgggc tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc
    16861 agcggtacat ctgtatcgtc gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg
    16921 ctcgcttgcg cccataacat ttcgcggttt tgtggagcat tttgttgctg tgccaacaat
    16981 tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt
    17041 tgtagatgga acgtcgcgtg ggtatagaga tcggctttat gggtgacggt cagttcaaag
    17101 ttattggtaa gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata
    17161 caacgcccca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc
    17221 agcgcatttg ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc
    17281 aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc
    17341 gtcggggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc
    17401 agcggcgaac ggttacgatc aatgccgtag cgttcgatct ctttatacag cacggttttt
    17461 gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca
    17521 aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac
    17581 accggccagt tggcgtaggt ggcggcacga cagcgccagc ggaacaattt ttggttatca
    17641 ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat
    17701 gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg
    17761 gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag
    17821 aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag
    17881 gggtgatcaa agcgcaaatc acgggcaaaa ccactgcgcg gatcagcggc cctacagggc
    17941 tataatcacg ggcgatttgt cggtcgagaa tgccaatgcc ctgagtgcgt tgttccatat
    18001 tcggcgtagc aagcagcatc tctaccagct ccgacacgtc ggagcgcatc tcgcgcacca
    18061 gtttcagcgt ttgcagacgt tgctctttga gaatatcgcg gcgaacacca ccaatcagat
    18121 tcagaccgta ggttttacgc gacccggtca gcaattccgc catcgtcatc gacttttccc
    18181 gcacgcggaa aaattgcata aagccggtat caaaaccaac aaaatggcag gagaggccaa
    18241 ggttgagcaa atgactgtgt agccgctcga cttccagcag aatcgagcga atagtatgtg
    18301 ctcgttgcgg cacctcaatc cccagtgcat tttcaaccga gttggtatag gccacactgt
    18361 gggcaaaacc gcaaatccca cacacgcggt ccgataagaa ggtcacttcg ttgtagccca
    18421 tccgcgtttc tgccagtttc tccatgccgc gatggacata aaacaggcgg taatcagcat
    18481 cgacaatttg ctcgccatcc acaaacaagc ggaagtgacc cggttcatag gaggtgatat
    18541 gcagcgggcc gacagggatc acccgcgcat cgctgttgcc ctcattgata aacgcatacg
    18601 tttcggaatc tgtcgtcggt tcagggcgca gtcgataatc catcgcatct ttgcgcagcg
    18661 gatgcatatc ttccggccag tcatcgggca acaccaggcg acgctgatcc ggcaggccaa
    18721 ccggaatcag cccgtacata tcgcgaattt ctcgctcgcc ccagaccgcg gcagggacgc
    18781 gcggtgtgac tgacggaaac tcccgactgt cggcatcgac cagtgccttc accacgatcc
    18841 agcatttttc ggccccttcc attgaaaggg cataataaac cgcgtaatga ccgttaagtg
    18901 tccgctcgtc gttgccaaac aggactggaa gccagccatc atgttgataa taaagataat
    18961 gtacaacgtc aggcagcaga ttgattttca ccgtaatggt gacctgttca ggcgtttgtc
    19021 gctcttcatc cagcaccgcg ccggggaact gcgttttcag ggcggcgaga atcgcttcgc
    19081 cacgatttga cgatgaatta acgttcactg tgtttctccg aaacagatgc ggtgcctgaa
    19141 ggcaaaaaat catgccaggt gctacgttgt gcaggcaggt cgtgcgtccc tgagaggact
    19201 atagtggaag cgcccgccag gatcctgatg acaggttgtg gaatatgcgt tcccatcgcc
    19261 agcatcatga ctagcagaat caccattggc gaggtggtca accagccgag atcaccccgg
    19321 ttaacggcct gcggcggttt cgccattaac acccgcgcag ccatccgtac caggcccgcg
    19381 agcaccagcg ttaacagcaa taacagcagg acgataatca gcaggtgatt acgtgccagt
    19441 cctgcggtaa cggtcataaa ttcgctaaga aaaatgttga agggcggcat ccctgccagc
    19501 gccagcgcac cgccgccaaa cagcacggcg gtaaatggca tgattttgag catcccacag
    19561 acgacgttga gatcgcgcgt gccgtacttg agcagtacat tgccggaacc gcagaacagc
    19621 agcgtttttg ccagactgtg gtttaaggtg tgcagcagcg cggcaaaaat tcccagcggc
    19681 ccgccaatgc ctagcgccac cgcgaccagc cccatgttct ccacgctgga gtacgccagc
    19741 agacgcttaa tgtcccgctg taccagaatg aaaaatgccg ccacggcaac cgacaacatg
    19801 ccgaagatga gcaacaaccg gttggggaaa tcgctgccga tggcctggca aataatgatg
    19861 taatagcgaa tcagcaccaa cagcgcgcag ttcagcaata cggcggagag cagggcgctg
    19921 accggactcg gcgcttcact gtgagcatcc ggcagccagg cgtgcatggg aaatagcccg
    19981 gttttggtac caaagccaat tagcacaaac acaaaggcca acagcattaa tgttgggtca
    20041 agcaaggacg attgcttaag aacctcgctc cagaatatcg ccatttctgc ctgcggcata
    20101 acgctggcgg cgttggcgta taccagcacg gtaccgaaca gaccaaaagc gacaccaaca
    20161 gtacaaataa tgatgtactt ccatgctgat tccagcgatg aacgctgacc gtaaatgcct
    20221 accagaaacg ccgagcttaa ggtggtggct tcgatcgccg cccacatcac aatcaggtta
    20281 ttgctggtaa caaccagcag catggtgaaa aaaaacagat ggaagaaacc gtagtaatcg
    20341 cacagtgtaa cgggtgaaag ctcgccgtgt gccacttcat gacgcatgta gccaatcgag
    20401 taaacaccgg tgagaaagcc aatcacacca agaatggcga ggaacaaacc gcccagacca
    20461 tcaatatgaa gccacagtcc cgcagcgaat atttctcctg catcagcggc agtttggacc
    20521 acccagagtg ccagaatcag cagcagtgtg atccctaagc tatgtaatac agtcaccgta
    20581 caagtcgcag agagcccccg tttccggcag gcaaaacaga gcagcgaaaa aagcagcggc
    20641 gtgagcagga gtaaagcgaa catcacagaa taactcatct cattatccct tcagcgcggt
    20701 caagttgttc acgtccagcg tgccgtgggt acgccatatt tttcttgcca gtaacaccat
    20761 cacaatgacg gcgaagatgg cgtcggtagc gatacctatt tccaccagtt ccggtgctcg
    20821 ccaggcaaga agcgccagca ccagatggga gccgttttcc atcaggcagt aaccaaaaat
    20881 ttgccgcagg atattgcgct ggctgacaat gcacagcagg ccaagcagaa aatgacctaa
    20941 cgctaccgcc agcgccggtt tcagcccggt agccatcggt agcttcacgg gctgaacgac
    21001 aaatgcgcaa agcaggacaa ttaacgccgc gagcagtgcc atcattgccg gaccgaataa
    21061 cgctttttcc gggatgttct ggggaatatt tcgtgcagcg taagtcatga ttaacggtac
    21121 cagcagcact ttggtgataa aggcgctggc ggaccagatc agcagttgct ctgcggcgaa
    21181 caggcacgag agagtggcga aaatagacac cagcaccagc gactggcagg cgtaaaatcc
    21241 gcatgacagg cgatagcttt tgacgctaat cacaaacagc gatgtcagca tcatcagtcc
    21301 cgccagatta tttacgatca tagaaccggt cattcatcat ctccttactg caaccaggtc
    21361 gcggcgatta cgctggaaat cagcgacatc acaatcagta caatcaacac caggcgcatt
    21421 gaccctggca gcggtgcggc atcggcgacg gcctcgctcg gtttgcccgg cacgacgcga
    21481 ccaaaccagc gaataaacca ggcgaaactg gcgaccgatt caatcatcag cagaatcatg
    21541 gcgggcagca ggatccagta ctccactgac aacgcaaaac cggcggcaaa cagcgggaat
    21601 ttactgaaga agccgttgaa cggcggcaca ccggtaattg ccagcgctgc cacgcagaaa
    21661 cccacgcctg gcaacggcag ggtgtgcaat acgccacgca gacgcggcaa caagcgcgtg
    21721 ccgcagctgt aactcagcgc acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg
    21781 ttgacgatgt aggcgatgct accctccagc gccagccgcg agccgaagat ggagagcgac
    21841 aagccgaaga acatccagcc aagttgagtg atggtcgacc aggccagcaa ccgcttcata
    21901 tcctgctgtg gcaaatacat cagaaagcca taaagaatgg tgaccagtgc catgaccatg
    21961 ccaacgccgc caatcacatg cgggatattg ccgccgtcga taatggcgcg ggcaaaaatg
    22021 taaacgccca ctttcaccat cgatgcggcg tggagatagt cgctgatcgg tgttggcgct
    22081 tccattgcgt ccggtagcca cgcttgcatc ggtagctggg ccgatttccc ccacgcggca
    22141 aacaggatgc cgccataaac cagataacgt gcgtcgccat gtaactcgct catcgcgcta
    22201 agcacaaacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa ccccaacgaa
    22261 ccgatatggg tgataagcaa cgcttttagt gctgaacgct gcgctttatc gctctggtaa
    22321 taactgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa ctgaccgagc
    22381 agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa aaatgcgtaa
    22441 taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata acccgtggag
    22501 tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt actcacgcga
    22561 tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa cgtgacggca
    22621 actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt ggtcagcgcg
    22681 gcgaacaaaa ccccccattc ggcggcccga cgttgtggcg aaaacgaaac gaccagtgcg
    22741 ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg tcagtgctcc
    22801 ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc caagccaagc
    22861 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    22921 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    22981 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    23041 atcacgacct gtttcagccc ctgcccccat ttcgctagcg ccagcccggc accggaatat
    23101 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    23161 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    23221 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    23281 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    23341 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    23401 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    23461 ggggaaacgg taataaatag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    23521 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    23581 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    23641 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    23701 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    23761 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    23821 gagacgaaag tcgccgcttt gcagatgctg tatttctttg accagccgtt gggtggcgct
    23881 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    23941 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    24001 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gagccatcgc
    24061 gttttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    24121 cgaacgacac cacagccagt aaaggcctgg cagcaaaggc atcgccagta gtaacagcaa
    24181 cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    24241 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    24301 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    24361 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    24421 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    24481 taccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    24541 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    24601 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    24661 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgtccgcgc cgagaaatag
    24721 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    24781 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgactg ccagcaaaat
    24841 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    24901 cgcatatagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    24961 ccacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    25021 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    25081 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    25141 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    25201 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    25261 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    25321 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    25381 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    25441 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    25501 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    25561 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    25621 aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt gatgccggaa
    25681 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    25741 tagaggatca gcgaccaggt taataattgc agggcatcca ttagtgttgc tcctgagtga
    25801 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    25861 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    25921 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    25981 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    26041 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    26101 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    26161 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    26221 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgattgtt gacgtcttcg
    26281 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    26341 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    26401 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaaggg tgccattctg
    26461 attaactcaa tctattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    26521 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    26581 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    26641 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    26701 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    26761 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    26821 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    26881 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    26941 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    27001 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    27061 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    27121 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    27181 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    27241 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    27301 ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    27361 gcgttcaatt aaatgcgggg agtctgcaac atcgacctga acccagacag atgctggcat
    27421 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc
    27481 tttcaacggt aacgttgatt caatcagagt gatggcattc catgaaccgg aaagcagcat
    27541 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    27601 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    27661 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    27721 gttcttaagg aaagcataaa aaaatcatgc atacaacaat cagaacggtt ctgtctgctt
    27781 gcttttaatg ccataccaaa cgtaccattg aggcacttgt ttgcacagag gatggcccat
    27841 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    27901 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    27961 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    28021 gatgacgctg gatctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    28081 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    28141 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    28201 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    28261 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    28321 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    28381 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    28441 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    28501 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    28561 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    28621 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    28681 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    28741 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    28801 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    28861 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    28921 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    28981 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    29041 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    29101 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    29161 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    29221 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    29281 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    29341 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    29401 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    29461 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcgtcgctg gaaaaagtgg
    29521 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    29581 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    29641 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    29701 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    29761 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    29821 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    29881 gtagttcgga taaggcgttc acgccgcatc cgaaaaaaca tccggcacac cagacagcaa
    29941 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    30001 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    30061 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    30121 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    30181 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    30241 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    30301 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    30361 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    30421 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    30481 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    30541 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    30601 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    30661 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    30721 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    30781 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    30841 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    30901 gacaggcgca acagctctgg cggtccgtca atgggcggtc ccggtttccg tctgccgagc
    30961 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    31021 ttaaccgggt tgatgaccgg gcagccggtt tctcaacaac aatcaacgcg gtcaattagc
    31081 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    31141 tggggacaac agttcgaaaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    31201 cgtggaatga cgcgcaccgg ctgcggggcg ggccagtcca ttatggggcc gttctattgc
    31261 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    31321 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    31381 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    31441 gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    31501 gggcatagta tgcagcagca aggcgttctg gaaactggcg atctggaaga ggcgctgaat
    31561 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    31621 gacagtttca cacatggcac ttctcagcaa cgctgcagct ggtttaaacg tggtttcgac
    31681 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaatttcc ggggcaggga
    31741 tggctgaact gactgcgctt tacacattaa cagcgcaaat gaaacgtgaa gggatctgcc
    31801 gcttgctggt gttgagcgga gaagagggtt ggtgttttga tcatgctctt aagttgcgtg
    31861 atgccttacc tggcgactgg ctgtggattt cgccgcagct agatgctgaa aaccactgtt
    31921 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    31981 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    32041 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    32101 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    32161 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    32221 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    32281 agcaactctt acagcagcta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    32341 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    32401 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    32461 ttcgctttat tgcaccggat gccttgttag ccagcgatga gcaagccgac tggctggtgg
    32521 tcgacgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg cgttttcctc
    32581 gaacgttgtt aaccactacg gtgcagggct acgaaggtac cggacgtggt tttttgctga
    32641 aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg atccgctggg
    32701 cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttaac gatgaaaact
    32761 tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg ttatggcgaa
    32821 gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac taccggacct
    32881 cgccgctgga tttacgccgg atgatggatg cactagggca acatttttta caggcggctg
    32941 gcgaaaacga gattgccggg gcgttgtggc tggtggatga gggcggatta tctcaagaac
    33001 tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc
    33061 tggcggcgca cggcagcaat ccactggcgg cgacattgcg cggaaggcgg gttagccgga
    33121 tagcagttca tccggctcgt cagcgggaag gcacagggcg gcaacttatt gctggagctt
    33181 tgcaatatac gcatgacctc gactatcttt cggtgagttt tggttacact ggggagttat
    33241 ggcgtttctg gcaacgctgc ggttttgtgc tggtgcggat gggtaatcat cgggaagcca
    33301 gcagcggttg ctatacggcg atggcgctgt taccgatgag tgatgcgggt aaacagctgg
    33361 ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag tggaatggcg
    33421 aaatgcttcc tgttgatcca ctaaacgatg ccatcctttc tgacgacgac tggcttgaac
    33481 tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc ttattgcgtc
    33541 tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gtgtttacag aaaaacgcca
    33601 gtgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta ctggttcgcc
    33661 agcgggaaga ggccgcgcag gcgctgttcg cactaaatga ggttcgcact gagcgtctgc
    33721 gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct cccatggtca
    33781 ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat gaaacatgac
    33841 cattttgttg ttcaaagccc ggataaacct gcacaacagt tgttactgct tttccacggt
    33901 gtcggggata acccggtggc aatgggggaa ataggcagct ggtttgcacc gctgtttcct
    33961 gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag
    34021 tggttttcag tgcagggtat tacggaagat aatcgccagg cgcgtgtgga tgccatcatg
    34081 ccgacgttta ttgaaacggt acgctactgg cagaaacaga gcggagtagg ggctaacgcc
    34141 acggcgctaa ttggtttctc acaaggcgcg attatggcgc tggagagcat taaagccgaa
    34201 ccgggccttg cttcgcgcgt cattgccttt aacggacgct atgcgagcct gccggagacc
    34261 gcgtctacag ccacgacaat tcacttgatt catggtggtg aagatccggt tatcgatctg
    34321 gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt gacgctggat
    34381 atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc cctggatcac
    34441 ttgcgttata ccattccgaa acattatttc gaccaagcgc taagtggcgg taagcctggc
    34501 gatgatgatg tgattgaaat gatgtaaaag acagattacc tggcctgata agacgcgtga
    34561 gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg ttaacgcctt atccggccta
    34621 cggttgagca ctcagcttac ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt
    34681 cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg agaaatttct
    34741 tatggtcgac gcgcttgaga tctttaatca cgttcagcag gacgccgacc aaaaagacaa
    34801 tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct tgcagaacca
    34861 ctcatcacgc gacgagctgt tctatgatac gttgatacat acgggcaagt agctgcaggt
    34921 cggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc ccgagttcca
    34981 ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga cgttccgcct gtggtcagta
    35041 gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc accagtttac
    35101 cgcgcgcggt caaaaatggc tgcccggaaa gccaccaatc caccgtatag cgcagttgat
    35161 gtttttcaag cagggcaagt acctgcgctt tgatcatctc atcagtcagt tcggtgctga
    35221 agcggaagtt aaactgcaca aacagttcac ccggaataac attgttactg cccgttcccg
    35281 cctgaatatt ggcaatctgc atactggtcg ccgggaagaa ttcattgccc tgatcccact
    35341 caatagccac taattcatta aggaaaggtg ctgcgcgatg taccggattg tcagccagat
    35401 gtgggtaggc aacatgcccc tgaacgccat gaatggtgag gttgcaggtt aacgatccgc
    35461 gacgaccatt tttcaccaca tcacctacca cttcgatact cgacggttcg ccgaccaggc
    35521 agtaatcgag acgctcatta cgtgccatta acgcctcgac gacttttacc gtaccgttgt
    35581 gggcactggc ttcttcatca gaagtgatca gaaatgccag tcgccccgta tggttgggat
    35641 gttgtgcgac aaaacgttct gctgctacca ccatcgccgc cagcgagcct ttcatatctg
    35701 ccgcaccgcg cccgaataac atgccgtcac gaatggtggg ttcaaacggc ggattgatcc
    35761 aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggct aacgtttcac
    35821 cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa atccatgcgt tcaacggtaa
    35881 aaccgatcgc ctgcaaacgt tcaatcaaca aagcctgaca tcctgcgtca tcaggactca
    35941 gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac atagactaca
    36001 cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata ggcttaccag
    36061 gcgcgtagag caatggacgt ttgataattg caggcatttc agtcattaat gccgccgcag
    36121 aggccgcatc ggtgatttta ttgcgggtag tttcgtccag tttacgccag gttgtaccac
    36181 gggtgttgag taacgcttcc cagcctaatt cgttgataaa accgttcaat aattcgctgt
    36241 ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct tccagccaac
    36301 ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc ataatgaatc
    36361 ctttttcgat ttatgagaat aaatactcat ttaagggtga aaacattttg cctgcaaatg
    36421 attatcagaa aatataacat tatcgaagta ttcaaattaa caactattaa ataatattaa
    36481 acatttttaa cgtaaacatt aagaatattc atcttgttat ctgtctgtat tttaaccaga
    36541 attaaataag tattagatgc cgtatgaata tctcgaaatg ttgctgtgta tcacatacaa
    36601 attatcaccg caggaccata gtatttacat aaggtttgtc ccccggaggt agtcatcgca
    36661 gcaatcgagt aaattagctt caccacggta tattttttcc gtagaatacc cataattata
    36721 agagaggttg ttatgattga acgtgaactg gggaactgga aagactttat cgaagttatg
    36781 cttcgtaagt aattcctgag acaaagtata aacagcaaga acccgctgag caggttctta
    36841 atcggaaagt tgaaaaaggc gacacattgg catgtcgcct tttttattgc ttattccggg
    36901 cgcggcttca gcgggaagcg gcggcgcacc agcacaaaga acagcggcac gaagtaaata
    36961 gccagaatag tagctgaaat catcccgccc attacgccag tacccaccgc atgctgacca
    37021 ccggaaccgg cacccgtgct ggttgccatt ggcaatacgc cgaagataaa tgccagcgag
    37081 gtcatcagaa tcgggcgtaa acgctgacgg caggcgtgga gcgtcgcttc aaacaggtcg
    37141 tggccttttt ggttcatctc gttagcaaac tcgacgatca ggatggcgtt tttcgccgat
    37201 aaaccaatga ccgttaacag gcccacctgg aagtaaacgt cgttttccag cccgcgcatc
    37261 caggttgcca gcagcgcgcc gattaccccc agcggcacga ccagcattac cgagaacggt
    37321 accgaccagc tttcatacaa tgccgccaga cacaggaata ccaccagcaa ggaaatggcg
    37381 tacagcgccg gagcctgcgt gccggaaagc cgctcctgat acgacatcgc cgtccactcc
    37441 agaccaaagc cgtttggcag ctgcttcact aacgattcca taatatccat cgccgtaccg
    37501 gtactgaccc ccggtgcggc ttccccaaca atctcaaccg cagaataact gttatagcgt
    37561 tccagacgcg gcgagcctgt ttcccagcgt gaggtcgcga aagcagagaa gggcaccatg
    37621 ccgccatctt tatttcggac ataccagaga ttgatgtcat ctggcagcat gcgatacggc
    37681 gcagctgcct gcacatagac tttcttcacg cgaccgcgat ccataaagtc attcacatag
    37741 ctcgaacccc aggcggtttg cagtgtgtcg ttaatatcgt cgatatcaac gcccagcgcc
    37801 tgagctttac gctggtcgat atcaatctgc aactgcggac tgtcatcgag gccgttatgg
    37861 cgcacacggg ttagctccgg gttttccgcc gccagcgcca gcaactgatt acgtgctgcc
    37921 atcagcgcat cgtgacccgc tccagcgtgg tcctgcaact ccatatcaaa atctgcagaa
    37981 ctaccaagac cgctaattgc tggtgggctg ctggcgataa cgcgagcttc tttaatttgg
    38041 ttaaacgcct tcgttgcacg ctcgataatg gcaaacgagg tgccggtctt actgtcgcgt
    38101 tcgctccagt ctttcaggcg gataaacatt ctcgccacgt tttgcccgtt acccccaggg
    38161 ccagaaccaa cggtggcaaa caccgacatg atgttgtctt tttcatggat gaagtaatat
    38221 ttctcgattt gctcaacgac tttcagggtc tgttgttggg ttgaaccgct gggcaattgt
    38281 accgaggtag taaacatgcc ccggtcttcc agcggtaaga acgacgtcgg caaacgcagg
    38341 aacaggaaca ccatgccgcc aagcagcagg acataaatca caatccagcg caggctacgg
    38401 tggagaattt tcgccacccc tttttcgtag cgttcggcgt tgcggttaaa catctggtta
    38461 aaccaggcaa aaaaaccttt ttgcccgtga tgctcagctt ttttcagtgg cttaagtagc
    38521 gtggcacaca gagccggagt gaggatcatc gctaccagta ctgacagcac catcgccgca
    38581 acaatggtaa tagagaactg gcgatagatg gcaccggtgg tgccgccgaa gaaggccatt
    38641 ggtacaaata ccgccgaaag caccatcgca atcccgacca gtgccccctg gatctgcccc
    38701 atcgatttac gtgtggcttc gcgaggagtg agtccttcct cactcataat acgttcgacg
    38761 ttttccacca ccacgatggc gtcatccacc agcagaccga tcgccagcac catcgcgaac
    38821 atggttaagg tgttgacgct gtaaccgaag gcgtaaagta cggagaaggt tcccatcaac
    38881 accaccggca cggcgatagt gggtatcagc gtggcgcgga agttttgcag gaacagatac
    38941 ataacgagga aaaccagagc gatagcttcc agcagcgttt tcaccacgtc ttcaatggag
    39001 gctttaacaa acgaggtggt ttcatacgcc accttgtatt ccagtccgtg cgggaaatac
    39061 tgcgccagct cgtcgagacg attgagcacc agctccgctg tcgccatttc gtttgcgccg
    39121 gaggccagtt ttacccccaa cccggaggct ggcttaccat tgaagcggct aagataatcg
    39181 tatttctccg cccccatttc gacggtggcg acatcgccca gccttacctc tgagccgtcc
    39241 tgattcaccc gcaaggtgat atcgcggaac tgttctggtg tttgcagcag tgactgggcg
    39301 ttaatggcgg cgttgagcgc ctgcttatcg acggaaggtg taccaccaag ttgcccaacc
    39361 gcaatctgcg cgttctgtga ctcaatggca tcagtgacat ctttagccgt catctggaaa
    39421 ctgttgagtt tcgccgggtc cagccagata cgcatggaat attgcgaacc ataggcatcg
    39481 atatccccga cgccattcac gcggcttaac gggtcctgaa tattactggc aacataatca
    39541 gcaatatcct gtttatccat cgagccatcg gtagagacga aggcaatggt cagaatgttg
    39601 gtatcgccgg ttttacgcac cgtcacgccc tgattttgca ccgcctgcgg taactttcgc
    39661 atggctgatt gcagctggtt ttgtacttgc tgcacggctt cgtccggatc ggtgcctgct
    39721 ttaaaactta aagtgacaga tgcctgaccg gtgccactgc tctgagatga catatacatc
    39781 agattatcga ggccggtcat attttgctcg ataacctggg tcacggtgtt ttccagcgtc
    39841 tgggccgatg cgccgggata gttagcggtc actcgcacat tcggtggcgc gagatcgggg
    39901 tattgttcaa cgggcaatga aaaaatcgcc agggtacctg tcagacacaa caggattgcc
    39961 agcacccagg caaaaatggg gcgatcaata aagaaattcg ccattaaaag aggacctcgt
    40021 gtttctgcgt attgttgtag gcttgacttg cttcactgta gcggcaaggt acgagccaat
    40081 cgtggagaaa aaaaggagtt aatgtaaatt atcatgagat tgctgacaca ctcgcttaca
    40141 ggtcaatatg ggcacgtttt tccctctcgc ctttaaaggg agagggccgg tgtgagggta
    40201 attatttaca cctcagccgg cgtgttcccc ttccccttcc cctcaccctc tgcggagcga
    40261 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccgccc
    40321 agccgttccg cgcgttcgcg catgatattc agtccataat gaccttcggg ttctttcggt
    40381 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    40441 acgcaactga tggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    40501 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gtgccagggt tggcagacgg
    40561 cagtcgaggg tcagtttagc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    40621 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    40681 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    40741 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagcacc
    40801 tgagccagcg agtcgtgcaa ttcgcgggcg atggtcgcac gttcttccat caacaataac
    40861 tgctgaaaat gcttctgcgc ctgattaaag tacaaaccgc gtcccagcat cgatgaaaca
    40921 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    40981 accgtctctt gcattgtcac cggtaaaatc tgcattggct gctcagggtt tggcttccct
    41041 tcgctaatcc gccagttatc cccaacgttc agctccagat attcagctgc ttcattgtcg
    41101 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggctggt gttcagcgcc
    41161 tgggaacact gcactgatac aacacctcca gacgacgctt ggcctcgtgg agatcgcggg
    41221 tcttttcttc tactgacgcc tccagcgaaa ggtacaattt atgcagctcg ctcgacattt
    41281 ggttaaaggt ttttgcaagc agaccaagct cattcggcag actggtatcc agcggcggcg
    41341 agtcgaactg cccgtgttca atacgctgac tggcggtaac cagctgattc agcggggcaa
    41401 ccacctgatg gcgtatgcgg cgcaaagtaa aaaagaccag cgtgaaaata ccgataccgc
    41461 cagccaggga aatcgccacc accagcagca ttttgcgttc agcgtagtgc tgtaaagcca
    41521 gcacgaacag gtctatctga ttaacataat tattaatatt ggcctgatac cacggcaaat
    41581 cgcccttgct gagccgatta ttcatctcca gccagttggc attcagatgc gcatagcgag
    41641 tttttactgc ttctggcaca taccagacgt tgaggttggt taataccggt gaatgcagtg
    41701 cctgctgaaa caactggcga tgtgcattga gttgtggact gccactttgc aagtcgtagc
    41761 ccaggcggta actctgcata cgcagcgatc cggcaatatt gatagcctca gcgtcgcgca
    41821 aactgctcgc cagagttagc agagcgatac ccgtggaaag aatcgacagc agcacaatgt
    41881 aaaaaaaggc ccgggccaga ctggccgaga cggggcgttt aacaatcaca cgcgtcttct
    41941 ccacaaaaaa tcattaatgt tccagttcgc tgaaacgcaa tttagtcagt gatgggatta
    42001 tttgatctgc cacagtttat agcgaaatag ttacttattc tgggcacatg aacattgccg
    42061 gttcccctgt aagtcgggta ataacaacac tcatataaag aataaggttt ttacaaccaa
    42121 aaaagaaggt cgttatgaat cgttttatta tggccaacag tcagcaatgt ctgggttgtc
    42181 atgcttgtga aatcgcctgt gtcatggctc acaatgatga gcaacatgtc ctgagccaac
    42241 accattttca tccccgaatt acggttatca aacatcagca gcaacgtagt gcagtgacct
    42301 gtcaccattg tgaagatgcg ccctgcgccc gtagctgccc taatggcgca atcagccacg
    42361 ttgatgacag cattcaggtc aatcagcaaa agtgtattgg ctgtaaatcc tgcgtggtgg
    42421 cctgtccgtt tggtacgatg caaatcgtcc tgacacccgt cgcggcagga aaagtaaaag
    42481 ccacggcgca taaatgcgac ctttgtgcag ggcgcgaaaa cggtcctgcc tgtgtcgaga
    42541 attgcccggc ggacgcgctg caactggtca ctgacgtcgc actctccggc atggcgaaat
    42601 cccgccgctt gcgcaccgca cgtcaggagc atcaaccgtg gcatgccagt accgctgcac
    42661 aagaaatgcc ggtaatgagt aaagtcgaac aaatgcaggc aacgcccgcg cgtggcgagc
    42721 cggataaact ggcgattgaa gcgcgcaaaa ccggttttga tgaaatttat ctgccatttc
    42781 gcgccgacca ggcacaacgg gaagcctcgc gctgccttaa gttcggcgag cacagcgttt
    42841 gtgaatggac ctgcccgctg cataaccata ttccgcagtg gattgaactg gtgaaagccg
    42901 gaaacatcga cgccgccgtc gagctttctc accagaccaa caccctgccg gaaattaccg
    42961 gacgcgtttg tccgcaagac cgtttgtgtg aaggtgcctg tactattcgc gatgagcacg
    43021 gcgcggtaac tatcggcaac attgaacgct atatttcaga tcaggcgttg gcgaaaggtt
    43081 ggcgtcctga cttaagtcat gtcaccaaag tggacaagcg ggtggcgatt atcggggcag
    43141 gtccggcagg gctggcctgt gcggatgttc tgacccgcaa tggcgtggcg gtgacggtgt
    43201 acgatcgcca tccagaaatc ggtggcttgc tcactttcgg cattccttct ttcaaactgg
    43261 ataaatccct gctggcacgc cgtcgggaaa tcttcagcgc gatggggatt cacttcgaac
    43321 tcaattgtga agtgggtaaa gatgtctctt tggattcgct tttggaacaa tacgacgcgg
    43381 tcttcgttgg cgtaggcact taccgttcca tgaaagcggg tttacccaat gaagatacgc
    43441 cgggcgttta tgacgcgctg ccgttcatca ttgccaacac taaacaggtg atggggctcg
    43501 aagagctacc ggaagagccg tttatcaata ccgccggact taacgtcgtg gtactgggcg
    43561 gcggcgacac cgcgatggac tgtgtgcgta ctgcgctgcg ccacggcgcg agtaacgtca
    43621 cctgcgctta tcgtcgtgat gaagctaaca tgccaggctc gaagaaagag gtgaagaacg
    43681 cccgcgaaga gggggccaac ttcgaattta acgtccagcc ggtggcgctt gagctgaatg
    43741 agcaaggtca cgtttgcgga attcgtttcc tgcgcacgcg tcttggcgag ccggatgccc
    43801 aggggcgtcg gcgacctgta ccgattgagg gcagtgaatt tgtcatgcca gccgatgcgg
    43861 tgattatggc gtttggcttc aatccacacg ggatgccgtg gctggagtcg cacggtgtaa
    43921 cggtagacaa atggggccgc atcatcgcgg atgtggaaag ccagtaccgt taccagacca
    43981 ccaatccgaa aatcttcgcc ggtggcgacg ccgtgcgtgg tgcggatctg gtggttaccg
    44041 caatggcaga aggacgtcat gcggcacagg ggattattga ctggctgggg gtaaaatcag
    44101 tcaaatctca ctgatagcct gcgcagacaa acccgacttc acagcgtaat ataattgttc
    44161 atttcgcgct gtggagtcgg tatgacgcaa caaatcaccc tcattaaaga caaaattctc
    44221 tccgataact atttcaccct gcacaacatt acttacgatc tcacgcgcaa agatggtgaa
    44281 gttatccgcc ataaacgtga agtttacgat cgcggtaatg gtgcgacgat cctcctgtac
    44341 aacgcgaaga aaaagagcgt ggttctgatt cgtcagttcc gtgtcgctac ctgggttaat
    44401 ggcaatgaaa gcgggcagct gattgaaacc tgcgccgggc tgctggataa cgacgaaccg
    44461 gaagtgtgca ttcgtaaaga agcgattgaa gagacgggct atgaagtggg cgaagtgcgc
    44521 aaattatttg aactgtatat gtcgcctggc ggtgtgactg agctaatcca cttttttatc
    44581 gccgaataca gtgacaatca gcgcgctaac gccggtggcg gtgttgaaga tgaagatatt
    44641 gaagtgctcg agctgccgtt cagccaggcg ctggagatga tcaaaaccgg cgagatacgt
    44701 gacggtaaga cggtgttatt gcttaactat ttgcaaatgt cacatttaat ggactgaaaa
    44761 atagcaataa tatttcgttg tttattattg gctcaatccg acaaatccga ttgagccgcg
    44821 ctacctgcgc aacgaagata cgacttgtgc tgtttgtttg aacttctggg gtcgtaccgt
    44881 ccatgcgcta tcgcattttc cttctctttt ttttcgcttt gttgccgacg tctttggtgt
    44941 gggcggcacc agcgcaacgg gcgttttccg actggcaggt cacctgtaat aatcaaaatt
    45001 tttgtgtggc gcgcaatacg ggcgatcata atggactggt gatgaccctg agccgcagcg
    45061 ctggggcgca taccgatgcc gttttacgta ttgagcgcgg tgggttgaag tcgccggatg
    45121 cgtctgaagg ggagatagcg ccacggctgc tattagatgg tgagccgttg gcattaagtg
    45181 gtgacaagtg gcggatttca ccatggttat tagtaaccga tgatacggca accatcaccg
    45241 cgtttttgca gataattcag gaagggaagg cgatcacctt acgcgacggc aatcagacca
    45301 tttctctgag cggcttaaaa gcggcgttgt tgtttattga tgctcagcaa aagcgcgttg
    45361 gcagtgaaac cgcgtggatc aagaaagggg acgaaccgcc tctcagcgta ccgcccgcgc
    45421 ctgcgctgaa agaggtcgcg gtggttaacc caacgccgac gccactctca ctcgaagaac
    45481 gtaacgattt gctggattat ggaaactggc ggatgaatgg tctgcgctgc tcgcttgatc
    45541 cgttacgtcg tgaggtgaat gtcactgcgc tgaccgatga taaagcactg atgatgatta
    45601 gctgtgaggc aggggcctat aacaccattg atttggcatg gattgtgtcg cgtaaaaagc
    45661 cgctagcctc gcgcccggtt cggttgcgtt tgccgttcaa caacggtcag gagacgaatg
    45721 aactggaact gatgaacgca acatttgatg agaaatcgcg tgaactggtg accttagcga
    45781 aagggcgcgg attaagcgat tgtggcattc aggcgcgctg gcgctttgac ggtcagagtt
    45841 ttcgcctggt gcgttacgcc gcagaaccta cctgcgataa ctggcatggg ccagacgcca
    45901 ggcccacgtt gtgatcaccc gttaaacaaa atcgcagatg cggccctaaa aagcgcacgg
    45961 accgtcccct cgcccttctg gggagagggt tagggtgagg ggaacataca gcatccggcg
    46021 atcaccatca ggcacctttc actcccagca ccttatgcgc ttttgccaca atattctcgg
    46081 cggtaaagcc aaagaacggg aacagcttat ccgccggagc agattccccg taacccgtca
    46141 tcccgacaat tgcccctttc agaccaacat acttgtacca gtaatcggca atacctgctt
    46201 ccaccgccac gcgagccgca acgttagaag gcaacaccga ctcccgatat tcctcatcct
    46261 gggcgtcgaa aatatcggtc gagggcaggg aaactacgcg tacattgcga ccttctcctg
    46321 ctaatttctc tgccgcttgc agggtaattt ccatctctga accggtggca atcagaataa
    46381 tatctggctt accgccgctg tctttcagca cataaccgcc acgagcaatc tctttaacct
    46441 gatccggcgt acgttccacc tgggccagat tctgccttga gaggatcagt gccgtcggtc
    46501 cgttgtggcg ctcaaccgcc agcttccagc ccaccgccgc ttccacctga tcgcacggtc
    46561 gccaggtgct gaaatttggc gttaagcgca ggctggccag ttgctcaaca gcctggtgcg
    46621 tcggaccatc ttcgcccagg ccaattgagt cgtgggtata aaccataatc tgccgcgctt
    46681 tcatcagtgc cgccatccgc gcggcgttac gggcgtattc aacaaacatc aggaacgtcg
    46741 cggtatacgg cacaaagccg ccgtggtgcg cgatgccgtt ggcgatagcg gtcatgccaa
    46801 attcacgcac cccgtagtga atgtagttgc ccgccggatc ttccttcagc gaaacagaac
    46861 ctttccagat ggtcaggttg ctgggagcca gatccgccga accgccgagc aactcaggca
    46921 gcatcggccc gtaagcgtta agcgtatttt gcgaagcctt acgggtagcg attttcgccg
    46981 gatttgcctg taactcattg atatatttct gagtcgtttt ctcccagtcc ttcggtaaac
    47041 caccgctcat ccgtcgggta aactcttctg ccagttgcgg atgagccttt ttataggcgg
    47101 caaacttctc attccagctc tgctgcgctt tttcgccttt ttcacgggca tcccaggcgt
    47161 gataaatctc tttagggatc tcaaatggcg gatggtgcca gcccagtttt tgccgtgcca
    47221 gcgccacttc ttcttccccc agtggtgcgc cgtgcgcctc ttccttacct gctttattcg
    47281 gcgaaccaaa gccaatcacc gtacggcaga taatcagcga cggcttatct ttcacgcttt
    47341 gcgcttcaag gatcgcttcc ttcaccgcct gcggatcgtg accgtcgatt tcatggatca
    47401 catgccagtg ataggcttca aaacgttttg ccgtatcgtc ggtaaaccag ccttctgttt
    47461 caccgtcgat ggaaataccg ttgtgatcgt aaaaaccaat cagcttgcct agtcccagcg
    47521 tgcctgccag cgaacagact tcgtgggaaa taccttccat caggcagccg tcgcccataa
    47581 acacataggt gaagtgatcg acgatctcat ggtctggctg gttaaactgc gccgccagtg
    47641 tacgctctgc tatcgccagc ccgacggcgt tcgccaaacc ttgtccaagc gggccggtgg
    47701 tggtttcaac gcctggcgta tagccaatct ccgggtggcc tggggttttc gaatgcaact
    47761 gacggaagtt cttcagttct tccagcggca ggtcgtaacc ggtcagatgt agcaaactgt
    47821 agagcagcat cgacgcgtga ccgttggaaa gaataaagcg gtcgcgatca taccaggttg
    47881 ggtcggtagg attatgttta agaaaatcgt tccacagcac ttcggcaata tcagccatgc
    47941 ccatcggcgc gccgggatga ccagaattgg ctttttgtac cgcatccata ctgagtgcgc
    48001 gaatcgcatt ggcaaggtct tttcgggaca tataacactc cgtggctggt ttatagtttg
    48061 gcggcaagaa gatcttccag tttgcgttga tcaacagcga acagacgaat gccttcagac
    48121 agtttttcta ccgccatcgc atcctgattg tgctcccaac ggaactccgc ttcgctcatg
    48181 ggtgctgggc gtgggaacgt ctgagaaggc gggattaatt tacgtaccac tggagaaact
    48241 ttttcctgca gctccttcag caaattcggt gcgatagtca gtcgatcgca gccggttaag
    48301 gcgaggattt gttcggtgcg acggaagctc gcgcccatca caatggtttc atagtggtgt
    48361 tgcttatagt agtcgtagat attgcgcacc gatttaacgc ccggatcttc ttccaccaca
    48421 tacgggtcca tcggcttgcg tgcctgatac cagtcataaa tacgccctac aaacggcgaa
    48481 atcagaaaaa cgcctgcttc cgcacaggcc cgcgcctggg caaaagaaaa cagcagcgtc
    48541 aggttgcagt taataccttc tttttccagc tcttcggccg cgcgaattcc ttcccaggtc
    48601 gaagccagtt tgatcaaaat gcgtgatttc tcaacgcctt gttgctgata caagtccacc
    48661 agatggcgcg ccttctcaat actcttttct ttatcaaaag agaggcgtgc atcaacttct
    48721 gttgacacgc aaccgggtac gattttgaga atttcagcac cgaaattgac cgccagtttg
    48781 tcacacgctg cgaccacctg ttgttcctgg gtcttgccat tttttttacc ccaggcgata
    48841 gcatcgtcta ttaaatgctc atattgtgat aatccggcag ccttgagtaa cagcgaagga
    48901 ttggtggtgg catccagggg atgataatgg cgaatggact caatatcgcc gctgtctgcc
    48961 acgacagtgg tgaactgttt gatgccgtct aactcgttca taggaaatac tccttgaaaa
    49021 gtaaagtgtt agatgagtgc gttaattcac acttctgaga aatttcgcta aacgcatcaa
    49081 aaaagcatag cagacaggca tggtattgct ggattaagca ggtaatatca gtgttatagg
    49141 attgttacca aaacattata ttaatggtga gagtttggac ttgctcaaag tctgtagact
    49201 ccggcagggt aataatgtgc gccacgttgt gggcaggggc tgttgcccac acactttatt
    49261 tgtgaacgtt acgtgaaagg aacaaccaaa tggatgacca gttaaaacaa agtgcacttg
    49321 atttccatga atttccagtt ccagggaaaa tccaggtttc tccaaccaag cctctggcaa
    49381 cgcagcgcga tctggcgctg gcctactcac caggcgttgc cgcaccttgt cttgaaatcg
    49441 aaaaagaccc gctaaaagcc tacaaatata ccgcgcgcgg taacctggtt gcggtgatct
    49501 ctaacggtac ggcggtgctg gggttaggca acattggcgc gctggcaggc aaaccggtga
    49561 tggaaggcaa gggcgttcta tttaagaaat tcgctgggat tgatgtattt gacattgaag
    49621 ttgatgaact cgacccggac aaatttattg aagtcgtcgc cgcgctcgaa ccgaccttcg
    49681 gcggcatcaa cctcgaagac attaaagcgc cagaatgttt ctatattgaa cagaaactgc
    49741 gcgagcggat gaatattccg gtattccacg acgatcagca cggcacggca atcatcagca
    49801 ctgccgccat cctcaacggc ttgcgcgtgg tggagaaaaa catctccgac gtgcggatgg
    49861 tggtttccgg cgcgggtgcc gcagcaatcg cctgtatgaa cctgctggta gcgctggggc
    49921 tgcaaaaaca taacatcgtg gtttgcgatt caaaaggcgt tatctatcag gggcgtgagc
    49981 caaatatggc ggaaaccaaa gccgcatatg cggtggtgga tgacggcaaa cgtaccctcg
    50041 atgatgtgat tgaaggtgcg gatattttcc tgggctgttc cggcccgaaa gtgctgaccc
    50101 aggaaatggt gaagaaaatg gctcgggcac caatgatcct ggcgctggcg aacccggaac
    50161 cggaaattct gccgccgctg gcgaaagaag tgcgtccgga tgccatcatc tgtaccggcc
    50221 gttccgacta cccgaaccag gtgaacaacg ttctttgctt cccgttcatc ttccgtggcg
    50281 cgcttgacgt tggcgcaacc gccatcaacg aagagatgaa actggcggcg gtacgcgcga
    50341 ttgcagaact ggcccatgca gaacagagtg aagtggtggc ttcagcgtat ggcgttcagg
    50401 atctgagctt tggtccggaa tacatcattc caaaaccgtt tgatccgcgc ttgatcgtta
    50461 agatcgctcc tgcggtcgca aaagcggcga tggagtcggg cgtggcgacg cgtccgattg
    50521 ctgatttcga cgtctacatc gacaagctga ctgagttcgt ttacaaaacc aacctgttta
    50581 tgaagccgat tttctcccag gctcgcaaag cgccgaagcg cgtagttctg ccggaagggg
    50641 aagaggcgcg cgttctgcat gcgactcagg aactggtaac gctgggactg gcgaaaccga
    50701 tccttatcgg tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca
    50761 aagcgggcgt tgattttgaa atcgtcaata acgaatccga tccgcgcttt aaagagtact
    50821 ggaccgaata cttccagatc atgaagcgtc gcggtgtcac tcaggaacag gcgcagcgtg
    50881 cgctgatcag taacccgaca gtgatcggcg cgatcatggt tcagcgtggg gaagccgatg
    50941 caatgatctg cggtacggtg ggcgattatc atgaacattt tagcgtggtg aaaaatgtct
    51001 ttggttatcg cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg
    51061 gtaacacctt tgttgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg
    51121 agatcacctt gatggcggca gaaactgtcc gtcgttttgg tattgagccg cgcgttgctc
    51181 tgttgtcgca ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg
    51241 cgctggaact ggtcagggat cgtgcgccag atctgatgat tgatggtgaa atgcacggcg
    51301 atgcagcgct ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt
    51361 ctgccaatat tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc
    51421 gcgtttccag ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg
    51481 ttcacgtgtt aacgccgatc gcatcggtgc gtcgtatcgt caatatggtg gcgctggccg
    51541 tggttgaagc gcaaacccaa ccgctgtaat tcattcttaa ctctcacgct taccctgaat
    51601 attcagggta agcggttttc ctgaaatata ttagcaaagc ttattactga gtttgcgaat
    51661 aataaaaaaa tcagtatata aaatatctcg atattatttt atttatattc atgcgtcgca
    51721 tatgaaagtt tattcataac agtaaatatc tctccattct tagtgatcta cctcaccttt
    51781 taaacgcgct tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa
    51841 gtttttcaca ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc
    51901 aaacaggtca cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc
    51961 ggcgttccgg atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg
    52021 atcgccggtg atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc
    52081 agcggtgcat tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc
    52141 gtcagcggtt taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac
    52201 gaggcattca tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta
    52261 tttaatgcgt tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat
    52321 gataagggcg atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc
    52381 ttaattacca cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg
    52441 gaaagtcggt tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc
    52501 atcagtaaaa cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag
    52561 acgggtttcg aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa
    52621 ctggtggatt atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac
    52681 agcgaatgat attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg
    52741 cgccagcatt attaccccgg aagcccgtga agtgtcggat ctactgggtt ttaccatcac
    52801 cgaatgtgat gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa
    52861 aaccgaaagc cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac
    52921 cgaaagcctg gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctgggcga
    52981 ttttgtcgtc gccagcgccg ggccgcacct cgaatccgtg atcgccggtc acggagccgg
    53041 ggcgcaaacc ctttctgaac aacggctgtg tcgggtactg aatatcgata tcggcggtgg
    53101 caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct gtctcaacgt
    53161 cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg gtttacgctc ataaaccggg
    53221 gcagatgatt gtggatgagt gcttcggtgc aggcactgac gcccgttcgc tgaccggcgc
    53281 gcagctggtg caggttaccc ggcggatggc ggcgctgatt gtcgaagtga ttgacggaac
    53341 gctttcgccg ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag gtgttacgcc
    53401 cgaaatcatt acgctttctg gaggtgtggg cgaatgttat cgccaccagc ccgccgaccc
    53461 attctgtttt gccgatattg gcccacttct ggcaacggcg ctgcatgacc atccgcgcct
    53521 gcgtgagatg aatgtgcagt ttccggcgca aaccgtatgc gccacggtga ttggcgcggg
    53581 tgcacatacc ctttcgctct ctggcagcac aatctggctg gagggcgtac aactgccgct
    53641 gcgcaatttg ccggtggcga tcccgattga tgaaacggat ctggtgagtg cctggcaaca
    53701 ggcgctgctt cagctggatc ttgatcccaa aactgacgtg tacgtgctgg cgcttcccgc
    53761 ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc aacgcgctgg tcgatttcgt
    53821 cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc gggcaggact ttggtaaagc
    53881 tctgggcatg ttgttgcgcc cacagctaca acaactcccg ttggcagtca ttgacgaagt
    53941 gattgtccgc gcgggggact atatcgacat tggtacgcct ctttttggcg gatcggttgt
    54001 gccggtgacg gtgaaatcac tcgcatttcc ttcctgaata cggtagttat caggtatggt
    54061 gcttggtggc tgggtgttag tgggattata tccggcagtg tatccggtta aggtgattgg
    54121 tctggcaaga tgattggatg cttttgtggt gaaaaatgtt ttggtgtttt agcataaccc
    54181 tgaagaaaga ataaaaataa aaaggaaaaa accaaaacca gaacagaaga gaaccggaaa
    54241 gagaagaaaa gagaaaatga aaaataaaaa ccaaaaaaga aagaatactt aaatcttcgc
    54301 atgttcttcg ggtggtacgc aagtttgcta gtctgactgc acaataaatt ttcgcttaga
    54361 aaataataag ttacataata ttctgctttt ttagataaat ataacccgaa aaaatcattg
    54421 ttaatcttat gcatttatta tttgattgta agttgtgatt attataattt agttattgtt
    54481 agtatgtttt ataatgctgg atgggtgatg cctctaatta gttgaatctg atgtataata
    54541 cgggcttttg aggttatctc atgaccagcg ttaatattca ttgtccccgt tgtcagtcag
    54601 ctcaggttta ccgccatggt cagaacccta aaggccgtga cagacttcgc tgccgtgact
    54661 gccaccgtgt gtttcagttc acttatactt atcaagcacg taagccgggt atgaaagagc
    54721 tgataactga aatggccttt aatggtgccg gggttcgcga taccgccagg acactgaaaa
    54781 ttggtagtaa caccgtcatc cggactttaa aaaactcgcg ccaaagcgaa taacttcttc
    54841 gcctgttgcc catgctgatg tggcgtttat ctgcgagctt gatgagcaat ggagctacgt
    54901 tggcagtaaa gcccggcaac attggctcgg gtacgcgtac aacaccaaaa caggcggtgt
    54961 actggcctac acttttggtc cccgaaccga tcaaacgtgc cgggagctac tggcactgcc
    55021 acccttcaac atcggcatgc tgaccagcga tgactggggc agctatggcc gggaggtgcc
    55081 gaagaataag catctgaccg gcaaaatatt cacccaacgc attgagcgta ataatctgac
    55141 gctacgcacc cgcattaagc ggttgggtcg taaaacaatc tgcttctcac gctcagttga
    55201 gatccacgaa aaagtgatcg gagcgtttat cgaaaaacac atgttctact aattggatgc
    55261 actacccagt atgtaactgg gtttgtccat aaaatgcacc ttactcagtt ggtgtccaac
    55321 ttttggggtg cagttcagaa ggacgggatt ttacggtgca ccggataaat ttaaaactgc
    55381 ctgatactga atgtatgagg tttcgtaatg ctcaattccc ctagtaccgt tttgtagatc
    55441 tccatacttc cactagtatt tatttgcaaa gttcctatat ttgaggagtt taataaattt
    55501 agctgtccca agtgattttt aatagagata gcctgatcat gtgcaggatt aataaatttt
    55561 atatccggtg caactgcttc aaaaaaattc tgaacaattg gatagtgagt acagccgaga
    55621 ataatttcat ttactggatg gagggaaatt aaatggttaa catgtttttt gatgtcattc
    55681 aagattgcag gagcattaag attaccttcc tcaattaaca ttgcaagatt tttgcttggt
    55741 tctccaaata catttattgt tgaattcaat ttttttatta gctctttgtg acaccccgtt
    55801 ttaatagtaa actcggtagc gataatacca acatttttat attgctggcg aatcacatct
    55861 tctactgcag gttcaatgat ggataagata ggaaacggaa agctcttagc atatccgctg
    55921 tatttttcgg attccaaagt tgaggatata gtattacatg cgacagcaac tagtttgaca
    55981 ttccttgact gtaaaaaagt cagcattttt ttagtcaaac tgatgatatc atcggcagaa
    56041 cggttgccat aaggtgcatt catattatct ccacaataga taatatcttc accaggaagc
    56101 aaattaatta tttcctttac cgatgtcaat ccacctacac ctgagtctat tacaccaatt
    56161 ggagattcag cactaaacat atttaacctc atcttttgta gcttatggaa aatttaatta
    56221 ctagttcttt tttcccaaga aaatggttcg aaatcagagt ttggatcttt ccagtcagat
    56281 tttcggaagg cgtaaattaa tattggaatt aataaaaata gggcaactaa tactaacaaa
    56341 taaagaacat agctggttgg actaccagtt gggatttgct cgggcggaat aaaacttaat
    56401 acaaaagcca gaaaagaccc taagaatcca agtgttgccc agaaaaataa agcaggggct
    56461 cgatatgggc gttttgtatc aggttgagaa tgtcgtagat agatagcact agcgaacatg
    56521 agcatataca taactaaata gagcataact gttaactgtg ataaaatctg aaaagctgct
    56581 tgaactgaag gcattatcac aaaagtaaca gaaagaactg agacaattat accttgtaca
    56641 aacattagat ggtgccccat tccatgacga ttggtttttt ggaaaaactt aggtaaataa
    56701 ccagctttcg cgatcgctaa tacacctgta ttaggccctg caatccaagt aaccacccca
    56761 cccagtacgc caattgctag cataaatgcc atcacagagc caagccatgg cactccagcc
    56821 cattcgaaca gcatatcgta ggcaaccaac aaactttgag ttagactaat tttatctgta
    56881 ggaataataa aggcgacccc taaagtactg agcaggaata ttgcaatagt acctatagaa
    56941 gcgatggtaa ttgcaattgg gtatttttta gttggattat ctaaatcgtt aacatggacc
    57001 gcattcattt ccatccctgc ataagcaaga aatatgctgg cggcaagaac aatattctgg
    57061 aagttagcta tattagggaa aagatcaccc caacctaatg ggatcaccgc tggtttaccg
    57121 ctgaaccaca gataggagaa accaagaata acaaggataa tagctggaat aattgtccct
    57181 ataataccgc accacttaga tacctgcgag aaaataccaa cgccacgtaa tgcaacacaa
    57241 atagctcccc agtaaacaaa tagtacgaaa aataaaatat accatttatt actggcaatt
    57301 gattctgcca cccccatatc aacatcaata aatgcaacag agactgcacc aaatgtcagt
    57361 actgttggaa agtaggggat gacttgtagc caggtcataa gcattcctac aaaaccccag
    57421 cgagccccaa atgcttctcc tatccatcga aatgcccccc ctttttgtgg atatgttgta
    57481 gctagctcag ccgcaacaaa ggcaacaggt actaaaaaca taattgcagc aaaggtatag
    57541 taaaaaatgg cacctaagcc gtattctgct tcagaaggta taaatcgtag acttactaca
    57601 gcagttatat tcatgatagc gagtgtcccc actgtcattt tcttactttt gggtgcgata
    57661 ttagcattag acatttttat actctcgaat atttttaaaa tagaaacata taattacgat
    57721 gtaaatataa aaactaatat taactttaac acttatctta aaaacactaa cgcaatctgc
    57781 tttacaaaaa taagtaatac ctaaagtcgt aacgacacca atagtgtttt catcgacatg
    57841 cttcaaaacc tctttagcat tcattatcag gtgatgatca tcaagtggaa tttcacacaa
    57901 tttcacatca cagtaacggc agaatttatg ccagcatatt tgcacaggat cgcaaatcag
    57961 gttcggttga tttaccttgt acttcgcgtt ttttacacca ctgccatttt aacgccaagc
    58021 ggccgagcat agcagcttca gatgaaccaa tggttgagca acctaaggtg tgtcgcgaat
    58081 cggggaaact ccacgaatcg gaaagcatac gcacacagcg accttcaggt tccgccattt
    58141 gtggatattc atctttataa atcatgtttt tatcaatagc taaattaagc aactcacgga
    58201 tttcatcatc aagccatgtt tgacagaaag tagctaagtt ttgtcgtgaa tccccatcaa
    58261 ggatgagctc atcacgtatg gcgctgaata catttcgaga tttcttctca tctacacgca
    58321 aatgatattt atgcaatata aatgaaagct caagtgagct atgaatatcg actgcttctc
    58381 tataacaaga attacctgat ttatttttca taataaacgc cactcaatac aatcagatta
    58441 aacattacaa acacaatctg gattttgcaa ttttaaaact atcaatttta gatatatttt
    58501 aattaatata aaaccataaa catatcaaaa aattacttta ttatggtcat ggtagatgtg
    58561 tttcaatgcc aacctggtct attcctacat atgtgagagc atatttacaa aaaaatcgtt
    58621 attgtgattt caacggtgat gctgaagtgg cacactaaat ttggccacct gaacagaggt
    58681 gatatgctca cctcagaaca acatgacgtg gtgttattct gctcagtact gttctggcaa
    58741 tgaatccagc cagtcctctg catcttcctt gtaacactcg tagatgcagt aattggtgaa
    58801 actgatgtat ttggttagta actctgcttc gttgttcctg tgcgctcttc tggcctgctt
    58861 acgtagttgt ttcgcttctg cccgctggcc ttcgagtatc tccagcctgt ctgcaatatc
    58921 ttgcttcatg ttcagctcct cgcgcgcgca tagtacttgt ccgaaaatta atcgcccgtc
    58981 ccatcattaa tatcgattcc cacgctgtgt aactggctat gaatgtctct gcgaatgaga
    59041 attaaacgct tgatgaactc catttcctcc gcgctctggt tgctcggccc cgatttcatg
    59101 tcgatgattt tattgatagt tccaagcgaa tattcagccc acagacgaag cgtttagcgc
    59161 ttcatggcct gtttgctcag tggcgaatgt ttcaggtcta agacatgttc tgcgagggtc
    59221 ttagcgatgc cctgcatgat gcgtagtgcc aacatgatga ttttctccgg acttgtgcta
    59281 atatagttcc cgaacattgt ttcgagactg cttatcctgt ttgtcttgtc cgatttgttc
    59341 attggtcaca ttgttaagtc agccggggta attcccggct ttcttttatc cgatcagtac
    59401 cgaatcccta tcgcattgaa tacgcgtttc cgttgctcag cttcaggatc ggtggccatt
    59461 tctggttctg gctccggcat agacggggcg gcatcaaaat gctcaatttc acgaccgaaa
    59521 tacgagttca ggtcaatacc tgccgaacta tccactccat caaaatagcg acgctgttcc
    59581 tcggtgagac agctacccag acgttcctca acctgcgcca taaactcaac atccggtgag
    59641 aagtactcat cgggattcgg agcaccgctc tgtttgtgca tctccatttt ctctgcgttc
    59701 gtttttagcc cggcaatgcg tttctccagt ccggcggctt taatagcgtc agcaacgatt
    59761 tgtgggatct gcggtttaat tgtttcgata acttcattac tgattaattt aaactgggcc
    59821 atacggatcg ctttaatctg tttatttagc agtgcgcgtt ccccttcgtt ggcggtttta
    59881 tcacgctgtg ctaccagttc agcgccttta tttgccagta cctcaaatgc gtccattatc
    59941 tttcccccct tttttcgatt atttaaaagc gcttattgac acgccaaaac gacgacttaa
    60001 ttagcaaatg agtcgccgct ttgcagtgcc ttccatatct tccggcattt cagactgaag
    60061 tttatcagct acttcatcaa caagtggacg aaccgtttcc acgaacttgt ccgcaatata
    60121 atcagcagaa cgcatcactt tctcatggaa ttcctcacgg cgttcctggg cggctgattt
    60181 aggggctgtc gtgcgctgaa aataactgtc taatgaatgc atggtattta ctactgtgtt
    60241 tatagtgatt gttcaattaa ttgcggttga agccattgtg tgtggcccag ccgaataagt
    60301 ttgcggacgt ttttatgtat ccggcggtgc ttttctttcg gcatcaggcg aatattcttg
    60361 atgcggttat cctgtttgtc caaattaacg tggtgcactt catataatgt ctcgtcataa
    60421 gcgccgtatg tgtatacata gcaggctttc tgataggtga gtgtcacttt gaatttacct
    60481 tcatgtaccg ttacaaccaa atgcccttta ttggtcatgc tgcctttgat aacgtctttt
    60541 gtttcacggc taaacaataa acccgcttca gggtcataaa tccatctgcg ccgcataata
    60601 tcggcacagc gttctaatct ttcgatattg gtcatgatgt ttacctcaaa atgtgatgcg
    60661 tgattattat cgttctgttc tgtacaacca ttctttgcat tcgtgctccc agaataattg
    60721 gcgagtaccg cgtggggtaa atggcaaagg gactccggtt gatggttttt ttagccagcg
    60781 cctcagcgtt ctggtcgata cattcgccat tgctgcgaac tgttctaatg ttattgcttt
    60841 cataaatacc tcgatacgaa aaagaagccg ccccacggag gaaagaagaa gcaaattttg
    60901 aatttccgtg aggcggtgga ttccgattcc ctgaacccat tacccatgtg atacttaata
    60961 ttataaacct tattttttct gttgacattt ttggccatat attgacagcg gtttttgtta
    61021 gcaaaaagtg ctatttatta aatcatcaaa gcaataaaag attatttgca cccgcaaggt
    61081 aatggcggga taatattagc gagggttagc cgccctcgac ctcaaagtga tctgttaata
    61141 actacaacga tatgtctcta tttcccgctt ctccggcggg ttttttttgc ctgcaaatca
    61201 ccacatcaga attaattaaa ttgataccgt ttaaaacgct cccaatcgca atataacggc
    61261 cttaacttct ggctatgcat ttgctcaggt gtgatcaaat tgattcgtca ggcgtggaat
    61321 aaggcaataa aaacgggcat tgcgcccgct tgtgttcact gcatcgaaaa taacttatcc
    61381 ataattcttt tctcctattc cctgcacatc atttcggcac ggtcccgatc ttcctgtgtc
    61441 ggattaaagt ccggcgttct gtatctgttg cctgaaatat accgtgcatg attgcggcga
    61501 cctgaacgcg gcattatcca gtattcgatg ttctcacgcg tggcaatgag taaaccatct
    61561 tctacaagcg tattcagggc gtgccatagc tgcttgtaag taacgcaacc ttcaccgaca
    61621 agataatcca gtgcgcacga caatccgctt actgttaact ctttggcggc gaaatttggg
    61681 tgttcagaaa ggaaatactc ctgaccctgt ttgctgaata cccaaaggat ctggcggcgc
    61741 aattcttcaa cgtcctggct ataagcgtga ttgatatgtc tttcagctac gaaagcgcgg
    61801 ccttgtttcc tgattagccc attactaacc atcgtcatga gtgagcggcg ggcggcgtca
    61861 acctggacgc gggtaacatt gccaccataa acgagatctg cggcttgtcg tgcggtgatt
    61921 gattcgtttt ttgccagata gtcgaggatc tgccgttgtt gttctgatat gcgcatgatg
    61981 tgtaaccccc tgattttcac aataaactta tctcttttct gcttaatgta tgtatctatt
    62041 atgcgctcaa tatcgaggcg gggcaatgtt gcgatcagtc ctgatcgcca ccattcaggc
    62101 gagtgagaaa aatttaatga aatcgcatcg attttaagcg ctctcaatcg ctctgtggcg
    62161 acgttaagtt ttcaggtaag caagttagcg cttatgtgtg ttcatcgcgt tctatgggcc
    62221 attcctttcg aatttggcgc ggtcatgata gttcgcggtg atcggcggcg gggataaagt
    62281 ttcctgagtg atcacttaag cctgctgtgc gtcgtgtgag tgtgctgtct gcacctggct
    62341 atacgctgct ttgctgctgt ttcctgtctg gtctgtatac gcgataaaaa ccattggtct
    62401 tgcagtgtgg cggtgtcgtt ggtttttatt gagcgcgtag cgcgatagaa ggtgcgctct
    62461 cagatgttta tgtgtctacc tatggtgagc gctccgtgcc ttacgtcgtt tcattgagca
    62521 ttggccccag gagacgagcg aagcgaagct ctccccactg ctaagctggt tttgttttta
    62581 tcctgcatgc tattccgctt aagccagcta gttaattcgt caaggaatga tttaaagagt
    62641 cctttaacat tatggtcaca atccgaacag ctttatcgtg aggatttgac ctagccgagc
    62701 ggcaatgagc gtagtcctgc tgcacagaag ttaaggtctg ctctggaggt tatcaggacg
    62761 taagcactag tctcatgatt ccgccgaaaa gacgaaaaat cttggcgctg atctgtattt
    62821 attatttctc tgtatttatt actttgggcc gcaaaaatgc ggctagtgag tgggcaaatt
    62881 tgcggctagt gagtgggcaa aaatgcccac ctattttttt tcagcgtcaa aatcatggca
    62941 aaatcatgac ctttctggat gcttcttgaa cagtaatcac gctttttctt gcactaacca
    63001 attgaataat aaaaggttct ggcaaatagg accgcatttt tgcggcttat tttgcttgca
    63061 gccagcgcca cgtctgggtt tgagctatca ctatcaaact ggttgtaacc gcgatgtgat
    63121 tattagttta ttgaggtaat gatctgatta tctgacatga aaaaaggggc atttgccccc
    63181 ctttgctgac ccgctgccta gtgtaccagg tagctgactt ttgcttctct ttcgtgctgc
    63241 tgtgccgtca ttgaggcctc ttctgctgtg ccttttgacg ggattcgcag tctgtaggtt
    63301 tttccacatt tgcccctcag tgcctggcgc atttcctgga taagtcccag ctttctcaac
    63361 gcttccaggt ggcgatctat cgtgtcaacg ctcttaccta acgcttttgc cattttggtg
    63421 cgggaatacc aaatctcata tttgtcgtta ccgcgatacc tgcacttgga taccatccac
    63481 gtatatagct ttaactcatc gcctttcagc ttgtgaaaca gcccgctttt gaagaggtaa
    63541 gccatagcgt tattgacggc tggaaggttg cgataatcgt tttgctggtc ttccgtttcc
    63601 acctgttccc gctcttcagg caactgctcc tctttactat caaaacgcaa atcttcttcc
    63661 gcttcttcgt taaaccatga agtcggcttg tctgtgttgg ctttcgctac tggcttgggc
    63721 attgccagcg ggtggcggca ttcttcttgc tgcgctgtga tttgttgtct ttgcagctcc
    63781 ttgaactcgg caatagccgc cgctttattc tgttcctgtt cctgttcctg ttcctgttcc
    63841 tgttcctgtt cctgttcctg ctcctgctcc tgctcctgct cctgctcctg ctcctgcgct
    63901 ggttctgcat tggctagcgc tggctcttca ttggctggcg acaagatttg atctatgcgc
    63961 acgaatccag ttgctggctt gtattcccag cgatacccat cgaagcggat gatgttcatg
    64021 ccatcacgaa aggcggcctg aacctgttcc ggaagggctt ggagtgtttg aagtgcttgg
    64081 agtgttgcaa ttttgtgttt gaccagtgtt tcgcctggtt tcgccacgta ggcggttgct
    64141 gattggtaca tggtattttt ttcctgtgtt gtttttgtgt ccccctagtg tgctgtagcc
    64201 tccagggtgt ttttgtgttg atcctatttt cgatccccga tccaccctgt tgcaaacacg
    64261 gctttaagtc attgatattt attcatttat tccaacgata ctggaataat tggaataatt
    64321 gatcccatcg gcaaagacaa aaaaagccgg acgaatccgg aaaaagtgaa tagttatttt
    64381 gaaggtgtcc agtgtttatc cggcctactc cggtggagtc gtcggaaaac gtgattaatc
    64441 attcattaca gttcaagcgg attgatacca tcagccagta tcgaatcaat cgcgtcggcg
    64501 tagtcctgaa gcatttgacg gcgcattttg agataggccg cttggttgta aactccccta
    64561 attcccttcg ggacgtgggc tagttggatc tcaatccaat cagggttata cctgccgctt
    64621 tcgttaagtg cggtgctgaa cgttgtgcgc cagccgtggc ctgtggctat tgacttgtat
    64681 ccgatacgct caagcgcctt gagtatcgcg ctggctgata atggcttatc catgaatcgg
    64741 cccgggaaca cgaaaacact gtatccgctg cgtgtgaact ggtcctgcaa tagctcgacc
    64801 gcctgccgtg atagcgggac cacatgcggg cggcgggcct tcatgacttc agcgggcaat
    64861 gtgattgttg cgtcctccag gtttatccat tcccaacggc acaaacgcag ttctgcggtc
    64921 cttaatgccg ttaatataag caaacgtatt gccgcctgaa tctggacgct cccggccctc
    64981 tcaagggctt tgtagaaggc cggaatttca ctaatcggta tccacgcgtt gtgaccaggt
    65041 cggcgggcct tcagtgccgt atcaagatct gcaaccggat tcctcgttac cagttcggtg
    65101 ataacggcaa acttgaagat ctcaccgatg cgctggcgta gcttgcgggc ctgctccggt
    65161 gcggtacgtt ctttcttctg gagtaatgcc agcacgtcac gggcggttat atcaacgatt
    65221 gaacgcttgc caagtaccgg ataaacgtct ttctccagtg ctgatttgac ctgctttgta
    65281 tagctctcac tccagccgcc taccctggta gccagccagt cctccgctac cgtcttaaaa
    65341 cctgccgcca tgttgagtgc atcaatggct ttctccttgc gtgccaaatc cattgggtca
    65401 attccctggg ctatcagtaa acggctctct gtggcgcgtt tgcgggcttc cttgaggcta
    65461 atcagcggat ataccccaat agcctgtaac cgctctttct tgtctatcca gtaccggaat
    65521 cgccagtatt tgctgccgtt gggatgcacc agcaaaaaca ggccgggcgt cagtgaagcg
    65581 gtgagtttgt attgcttgtc ctttggttgt gcggcgtcta tggcctttgc tgtgagtggt
    65641 gttttgctca aaaccggata cctcgatcta acgactcgat aaccccgctc atgtatccgg
    65701 cgagttcggt tgcgttcggc aggataagta acgggcgtgt taaggaaatc accttacatg
    65761 acaagggcta acgatgaaat agaattgttt gcaggctgtg agaaatgtta ctcatcactt
    65821 cttcctgagg gaacgactta tgaaactaaa gaccacattg ttcggcaatg tatatcagtt
    65881 taaggatgta aaagaggtgc tggctaaagc caacgaactg cgttcggggg atgtgctggc
    65941 gggcgttgca gcggcaagct cacaggagcg cgtggcggca aagcaggtgt tgtcggaaat
    66001 gaccgtagcg gacatccgca ataatccggt gattgcctat gaagatgact gcgtgacgcg
    66061 gctgattcag gacgacgtta acgaaacggc ctacaaccag attaaaaact ggagcatcag
    66121 cgaactgcgt gagtatgtgc tgagcgatga aaccagcgtg gacgacattg cctttacccg
    66181 caaagggctg acctcggaag tggtcgcggc gatagcgaag atttgctcca acgcggacct
    66241 gatctacggc gcgaagaaaa tgccggtaat caaaaaggcc aataccacca tcggtattcc
    66301 gggcaccttt agcgcccgtt tgcagccgaa cgatacccgt gacgacgtgc aaagtatcgc
    66361 tgcgcaaatc tatgaagggc tttccttcgg ggtgggcgat gcggtgatcg gcgttaaccc
    66421 ggtaactgac gacgtggaaa acttaagccg cgtgctggat accatttatg gcgtgatcga
    66481 caaattcaac atcccaactc agggctgcgt actggcgcac gtcaccaccc agatcgaagc
    66541 gattcgtcgc ggcgctcctg gcggactgat tttccagagt atctgtggca gcgaaaaagg
    66601 gctgaaagag tttggcgtgg aactggcgat gctcgacgaa gcgcgcgcag tgggcgcaga
    66661 gttcaatcgt atcgccgggg aaaactgcct ctacttcgaa accggacaag gctctgcgct
    66721 atccgctggc gctaacttcg gcgctgacca ggtgacgatg gaagcacgta actatgggct
    66781 ggcgcgtcat tacgatccgt ttatcgtcaa caccgtggtt ggctttattg ggccggagta
    66841 tctctacaac gaccgccaga ttatccgtgc tggcttagaa gatcacttta tgggcaagct
    66901 gagcggcatc tctatgggct gtgacggctg ctacaccaac cacgctgact ctgaccagaa
    66961 cctcaacgaa aacctgatga tcctgctcgc caccgcaggc tgcaactaca tcatggggat
    67021 gccgctgggt gatgacatca tgctcaacta ccagaccacc gcattccacg ataccgccac
    67081 tgtgcgtcag ttactcaacc tgcgcccgtc accagagttt gaacgctggc tggaaagcat
    67141 gggcattatg gcaaacggtc gcctgaccaa acgggcgggc gatccgtcac tgttcttctg
    67201 atgacgcgga gataacacat catggatcaa aaacagattg aagaaattgt acgcagcgtg
    67261 atggcgtcaa tgggacaagc ggcccccgcg ccgtcagaag caaagtgcgc caccaccaac
    67321 tgtgcggcac cggtgacctc ggaaagctgt gcgctggatt taggttccgc tgaagcaaaa
    67381 gcgtggattg gtgttgaaaa cccgcatcgc gcagacgtat taacagaact gcgccgcagc
    67441 accgtggccc gcgtttgtac cggtcgtgcc ggtccgcgtc cacgtactca ggcgctgctg
    67501 cgtttcttgg ccgatcactc tcgttcgaaa gataccgtac tgaaagaagt gccggaagag
    67561 tgggtgaaag cgcagggctt gctggaagta cgctctgaaa ttagcgacaa aaatctctac
    67621 ctgactcgcc cggatatggg ccgccgcctg tgtgcagaag ctgttgaagc gctgaaagca
    67681 cagtgtgttg ctaatccgga cgtacaggtc gttatttctg atggcctgtc aactgatgcc
    67741 atcaccgtca actacgaaga gatcctgccg ccgctgatgg cgggcctgaa acaggccggg
    67801 ctgaaagtcg gcacgccatt ctttgttcgt tatggtcgcg tgaagattga agatcagatc
    67861 ggtgagatcc tcggcgcgaa agtggtgatc ctgctggtgg gggaacgtcc ggggttaggg
    67921 cagtcagaaa gcctctcctg ctacgccgtt tactcgccgc gtatggcgac caccgttgag
    67981 gccgatcgca cctgtatctc taacattcac cagggcggca cgccgccggt tgaagccgca
    68041 gccgtcattg tggatttggc gaaacgtatg ctggagcaga aagcatccgg catcaacatg
    68101 acccgataag gaggcatcat gccagcttta gatttgattc gaccgtcggt aaccgccatg
    68161 cgggtgattg cctccgttaa cgctgaattt gcgcgtgaac taaaattgcc gccgcatatt
    68221 cgtagcctcg ggctgatttc tgccgattct gacgacgtaa cctacattgc ggctgacgaa
    68281 gcgaccaaac aagcgatggt cgaagtggta tatggtcgct cgctgtatgc gggagcggca
    68341 cacggcccgt caccgactgc cggtgaagtg ctgattatgc ttggtgggcc aaacccggcg
    68401 gaagtgcgtg ccggtctgga tgcgatggtt gcacatattg aaaatggcgc agctttccag
    68461 tgggccaatg acgcagaaaa caccgccttc ctggcgcatg tagtttcgcg tacaggttct
    68521 tatctttcat caaccgccgg gatcacgctt ggcgatccga tggcgtatct ggtggcaccg
    68581 ccgctggaag cgacctacgg cattgatgcc gcgttgaaat ctgccgacgt acagctggtg
    68641 acctacgtcc cgccaccgtc tgaaaccaac tactcggcag catttttaac cggtagccag
    68701 gccgcgtgta aagcagcctg taacgccttt accgatgcag tgctggaaat cgcgcgtaat
    68761 ccaatccagc gtgcgtaacg gaggttgccg atgatcaatg cactgggatt gctggaagtg
    68821 gacggcatgg tcgccgcgat agatgcagcg gatgccatgc tcaaagcagc taacgttcgt
    68881 ctgctcagtc acgaagtgct tgaccctggt cgcttaacgc tggtggtgga aggcgatctg
    68941 gcggcgtgtc gtgcggcgct ggacgcaggt tgtgctgccg cgatgcgtac cgggcgtgtc
    69001 atcagccgta aggagatcgg tcggccagac gatgacaccc agtggctggt tactggcttt
    69061 aaccgccagc cgaagcaacc cgtaaaggaa cccgacgcgc cagttatcgt cgcggaatct
    69121 gctgacgagt tgttggcgct gttaacatca gtatgtcagg gaatgacggc aggagaagtg
    69181 gccgcccact ttggctggcc gctggaaaaa gccagaaatg cgctcgaaca gctcttttct
    69241 gccgggacgt aacgtaaacg cagtagtcgt tatcgtctca agccccatta acctgtcgga
    69301 ggtgccgggt gtcctacaac acccggcatt aacatcatga aaaagacccg tacagccaat
    69361 ttgcaccatc tttatcatga acccttaccc gaaaacctga agctcacgcc gaaggtcgaa
    69421 gtggataatg ttcatcaacg acagacaacg gatgtctatg aacatgcttt gacgattacc
    69481 gcctggcagc agatttacga tcagctgcat ccgggcaagt ttcatggtga atttacggaa
    69541 attctactcg atgatattca ggtttttcgt gaatacaccg gtctggcgct gcgtcagtcg
    69601 tgcctggtct ggccgaactc gttctggttt ggcattccgg cgacgcgcgg tgagcaggga
    69661 tttatcggtt cgcaatgtct gggaagcgca gaaatagcaa cgcgccccgg tggaaccgag
    69721 tttgaattaa gtacgccgga cgattatacg atcctgggcg tagtgctgtc tgaagatgtc
    69781 atcacctgtc aggctaactt tttgcacaat ccggatcggg tattacatat gctgcgtagc
    69841 cagtcggcgc tggaagtgaa agagcagcat aaagccgcgc tgtggggctt tgtccaacag
    69901 gcgctggcga cgttttgcga gaacccggaa aatctccatc agcctgcagt gcgaaaagtg
    69961 ctgggggata atttgctaat ggcgatgggg gcgatgctgg aagaagcgca accaatggtg
    70021 acggcggaaa gcatcagtca tcagagttac cgtcggttgc tttcccgcgc ccgtgaatat
    70081 gtgctggaaa acatgtccga accggtgacg gtgctggatt tgtgtaatca actgcatgtc
    70141 agtcgccgca cgctacaaaa cgcgtttcac gctattttag gcattggccc aaacgcgtgg
    70201 ctgaaacgca ttcgcctgaa cgccgtacgc cgcgaactga taagcccgtg gtcgcaaagc
    70261 acaacggtaa aagacgccgc catgcagtgg ggattctggc atctggggca atttgccacg
    70321 gattaccagc agctgtttgc cgagaagccg tcgttgacgt tgcatcagcg gatgcgggaa
    70381 tgggggtgag ggagttacac ccaatccctg accttaataa actcacttaa cgccgcttct
    70441 gggctgccat cttttggctg ataatcatac tcccagcgta ccagtggcgg cattgacatc
    70501 aggatcgact cggtgcgccc gccagtttgc aggccaaaca gcgtgccgcg atcccagacc
    70561 agattgaact cgacataacg accgcgacgg tagagctgaa aattgcgctc gcgctcgccg
    70621 taggccatcg ctttacgtcg ctctacaatt ggtaaataag cgtcggtgta gcctttgcct
    70681 accgcctgca taaaggcaaa acagtggtcg aaatctggcg tgttcagatc atcaaagaac
    70741 agcccgccaa tgccgcgctg ttcgttgcga tgtttgaggt agaagtaatc gtcgcaccac
    70801 tttttgtaac ggggataaac gtcttcacca aatggctggc acaggtcacg ggcggtgcgg
    70861 tgccagtgaa tggcgtcttc ttcaaaacca tagaaagggg ttaaatcgaa gccgccgcca
    70921 aaccaccaaa cgggctctgc acccggtttt tcggcaataa aaaaacgcac attcgcgtgg
    70981 ctggtgggaa catacgggtt atgcggatgc actaccagtg aaacgcccat cgcctcgaaa
    71041 ctgcgcccgg caagttccgg gcgatgagcg gtggcggaag caggcatcgc ctcaccgtgg
    71101 acgtgagaaa agttgacgcc cgcctgttcg aaaacaccac cattacgcaa cacccgactg
    71161 cgcccgccgc cgccagcttc gcgctgccaa ctatcttcga caaattctgc gccatcgacg
    71221 gcggtcagct gctgacaaat cgtatcctga aggttgagca gaaactgttt aacctggtgt
    71281 gcgtcgggtt tcataactta tcgctttttc gaatgtgctt tctggttgtc gaaccagtgg
    71341 aaataactga tcacgccttc agcaatcgct gtggcgattt tctgacgaaa cgccgccgtg
    71401 cctaacagcc gctcttcttc cgggttggtg ataaacgagg tttccaccag caccgaagga
    71461 accgacggcg atttcaacac cacaaacgcg gcttgttcgg tgttgcggct gtgcagttta
    71521 tgcaccggct taatcttttt cagaatatgc gagccaagcg tcaggctgtt tttaatagta
    71581 tcggtttgca ccagatcaaa cagtacttgc tgcaataggt gatccttgtc agtcgccttt
    71641 ttaccggcaa cttcatcggc gcggttttcg cgttcagaca ggtatttcgc cattgcgcta
    71701 cttgccccac ggttagagag ggcaaatacc gaagcaccgg cagctttcgg gttggtaaag
    71761 ccatcggcat gaattgacat aaacagatct gcgccatgtt tatgggcgat ttcaacgcga
    71821 tcgtaaagtg ggataaacgt atcgccagaa cgcgttaaac gcgcatcaat cccatgatta
    71881 cgcaaaatgg aacggacgtt tttagcaatc gccagcacca catgtttttc tttcgaaccg
    71941 ttgcgtccga tcgctccggt atcaattccg ccgtggcctg gatcgagaac aacgacacgt
    72001 ttgccgccag atttttttgc tttcggcttg ctgtgtccgt tgctggtttt taaaggttcg
    72061 tctttggcaa tggcttgcga cattcctgac aacgtcaggg cagctaaacc ggctttcagc
    72121 acctggcggc gcgaagtgag tgtttttagt ggtttaaaag tgctcatacg gcctgagttg
    72181 taataataaa gttccagatg ttatatcgta tcgcgtaatc cgttacgacg gtttgctgtt
    72241 gagaattgtt ttacttttca tttcaatagt tgacaactcc ccattatgcc actttttcaa
    72301 ccgattttcg tcagatattg gctaattcga ccgttcacgc cataatcagc cttttaaccc
    72361 gcaaaaaggt aacgaatacc atggagatac gcgtatttcg ccaggaagat ttcgaagagg
    72421 tcatcaccct ttgggagcgt tgcgacttgc tgcgtccgtg gaacgatccg gaaatggaca
    72481 tcgagcgtaa gatgaaccat gacgtcagtt tgtttttggt cgctgaggta aacggtgaag
    72541 tggtcggaac ggtgatgggc ggttatgacg ggcatcgcgg gtctgcttat tatcttggcg
    72601 tgcatccaga gtttcgtggg cgtggaattg ccaatgcgtt gcttaaccgg ctggagaaaa
    72661 agctgattgc tcgcggctgc ccgaaaattc agatcaacgt gccggaagat aacgacatgg
    72721 tgctcggaat gtatgaacgc ctgggatatg aacacgccga cgtgctgagt ctgggtaagc
    72781 gtttgattga agatgaagag tactgagttc catcctgtcc attatgatgc gcacggacgc
    72841 ctgcgtttac ccttgctctt ctggcttgtg ctattgcttc aggcgcgaac ctgggtgctg
    72901 tttgtcattg ccggtgcgtc gcgcgagcag ggtactgcgc tgctaaatct gttttatccc
    72961 gatcacgata atttctggct ggggctgatt cccggtattc cggcggtgct ggcgtttttg
    73021 ttgagcggtc ggcgggcttc gtttcctcgc acctggcatg tgctctattt tctgttgctg
    73081 ttggcgcagg tggttttact ttgctggcaa ccgtggctgt ggctgaacgg tgaatccgtt
    73141 agcggtatcg gtctggcgct ggtagtggcg gatattgtgg cgttaatctg gctgctgacc
    73201 aatcgacgtt tacgcgcttg ttttaatgaa gaaaaagaat aacggcactt tttggtgaat
    73261 ttgcactcca agcaacgtta ttgaataacc aaaggaagtg acatgaaatc gctgcgttta
    73321 atgttatgcg ctatgccgtt gatgctgacc ggctgttcca cgatgtcgtc agttaactgg
    73381 tctgccgcta acccgtggaa ctggtttgga tcgtccacca aagtgagcga gcagggcgtg
    73441 ggtgaattaa cggcgtccac accactgcaa gaacaagcca ttgctgatgc gcttgatggc
    73501 gattatcgcc tgcgcagcgg aatgaaaacc gcgaacggca acgtggtgcg cttttttgaa
    73561 gtgatgaaag gcgacaacgt ggcgatggtg attaacggcg atcaaggcac gatcagtcgc
    73621 attgacgtgc tggatagcga tattcctgct gacaccggtg ttaaaatcgg tacaccgttt
    73681 agcgaccttt acagcaaagc atttggcaat tgccaaaaag ctgatggtga tgataatcgt
    73741 gccgtcgaat gtaaagccga aggcagtcaa catattagct accagttcag cggggaatgg
    73801 cgtggtcctg aagggttaat gccttcggac gataccctga aaaactggaa agtcagtaaa
    73861 attatctggc ggcgttaatt tgcgtctgaa caaaccgccg tcgcgaaaaa acacgtaaaa
    73921 tagcgctcaa caatgccacg gattgcgtgg cattcttatt ttcaggagga acaatgtctc
    73981 aggttcagag tggtattttg ccagaacatt gccgcgcggc gatttggatc gaagccaacg
    74041 tgaaagggga agttgacgcc ctgcgtgcgg ccagtaaaac atttgccgac aaactggcaa
    74101 cttttgaagc gaaattcccg gacgcgcatc ttggtgcggt ggttgccttt ggtaacaaca
    74161 cctggcgcgc tctgagcggc ggcgttgggg cagaagagct gaaagatttt ccgggctacg
    74221 gtaaaggcct tgcgccgacg acccagttcg atgtgttgat ccacattctt tctctgcgtc
    74281 acgacgtaaa cttctctgtc gctcaggcgg cgatggaagc ctttggtgac tgcattgaag
    74341 tgaaagaaga gatccacggc ttccgttggg tggaagagcg tgacctgagc ggctttgttg
    74401 acggtacaga aaacccggcg ggtgaagaga cgcgccgcga agtggcggtt atcaaagacg
    74461 gcgtggatgc cggcggcagc tatgtgtttg tgcagcgttg ggagcacaac ctgaagcagc
    74521 tcaaccggat gagcgttcac gatcaggaga tgatgatcgg gcgcaccaaa gaggccaacg
    74581 aagagattga cggcgacgaa cgtccggaaa cctctcacct cacccgcgtt gatctgaaag
    74641 aagatggcaa agggctgaag attgttcgcc agagcctgcc gtacggcacc gccagtggca
    74701 ctcacggtct gtacttctgc gcctactgcg cgcgtctgca taacattgag cagcaactgc
    74761 tgagcatgtt tggcgatacc gatggcaaac gtgatgcgat gttgcgtttc accaaaccgg
    74821 taaccggcgg ctattatttc gcaccgtcgc tggacaagtt gatggtgctg taagcgtttc
    74881 tgattcattg attaaaggcc aggcagtaac attactgact ggccttttta ttactgcttt
    74941 aacgccgcat atacttgatc aactaccttc tgtcagaaca gggtattttg cggcgctgac
    75001 gctacaacta acagccagca gagaaagata gagcattgtc cgtttcataa tatcagtcca
    75061 gattgacgtt acggcagcca aagagcgtgg tgaaaataaa cccgcaaacc catgccacca
    75121 gaatcccgcc agcataaacc gccattgccg gaaggatgcc ttgtgcggaa gtcatcagcg
    75181 gcagcgccac cagaccagac ggcccaaagg cgctgtttaa gcccatcggc agaccccacc
    75241 aggctatcaa tccgataaac aaaccgcccg ccgcgccgcc taaacaggcg gtaacaaacg
    75301 gcttcatgcg gggcagggtg acaccgtaaa tcagcggttc accaacgccc agcaggccgg
    75361 gaataatcgc cccgcgtacc tgactgcgta gcgcactgtg cggttgcgcc cgccagtaga
    75421 gtgccagcgc cgcgcccacc tggcccgcgc ctgccattga aaggatggga aataagctgt
    75481 tgaatccctg gctgtccatt aacgcgaggt aaacaggaat aaagccctga tgcacgccaa
    75541 ataccacggc gatcaggaac agcccggcta aaaccgcaca accgaacgga ttactgttca
    75601 ggtgcataaa cagccatgac ataccttcga ataaccagcc acccagcggc ataatgatca
    75661 ggtacgcaag cgtagcggtg atcagcaggg tgattaacga ggtcagcagc atgtcgagat
    75721 cgtccggcat aaagcggcgc accatgcctt caatgcgggc gcaggccctc gcggcaatca
    75781 gcacgccgat aatattgccg cgcggatcga tgggcagacc aaagaaatcg tgaaaaccgg
    75841 cgtagtaacc ggtggttgca gcggggttat aaccgagcaa aaagagtgcg gcaataattg
    75901 cgccattgac gcccgtgccg ccaaatgcct gggcggcgtt atagcccacc agaatcacta
    75961 ggaaggtgaa caaacctttg ctgaacacct tcataaaatt cagcgcgtcg ggtagcgtgc
    76021 cctgagcatc tgccggaacg tgcatcaccg tggcaattaa cgtcgctatc cccagcagca
    76081 gaccggcggc aataaaaccg gggatcagcg gcgtgaagat ggtggcgaat ttggcgagaa
    76141 attgttgcac gccggaggtt tgtttggctt ttaactgacg tttgttctgg gcggcgattt
    76201 ctgcggcatc ctgtaccggt gcctcgccca gcagctcgct catcgctttt gcggcacgat
    76261 gcgctttacc aggcccaaaa acgacctgca cctggtcgct ggtcaaaatg acgcccttca
    76321 cgccttccag cgttttgata ttgggatcaa ccagtgaact gtcatgtaca cccagacgca
    76381 ggcgcgtcat acagttacca caactggcga tatttcccgg tccgccgaca cgggtaagaa
    76441 tggtgttcag aagttcactg ctgatctctt tggccatggg ttattccttg tctaaaacct
    76501 gacgaataaa gccgccgtgt tgatccaggc gttttttagc ttctgcggca tcgagatttt
    76561 tcagcaccat cacaatggcc gttttacagt tgcgctcgca ggcaattaac gccgcttccg
    76621 cttgttcggc attacatccg gtggcgtttt taacgatatt gacctggcgc acatgcagtt
    76681 tttcgttggt ggcgaccaca tcgaccatca ggttgccgaa cactttgccg gatttaatca
    76741 tcagcccggt ggaaagcata ttgagcacca gtttctgcgc tgtgcctgct ttcatccgcg
    76801 aagaaccggt aacaacttcg gcacctacaa tcggtgtaat ggcaaactca gcggtggttg
    76861 aaacggcgct ccccggatta caggaaattc ccactgtgcg gcagccgagc tggcgtgcgt
    76921 attccagtcc ggcaatcaca tacggcgtgc gaccgctggc agcaatgcca accaccacat
    76981 cctgtgccgt taaattaata tttttcagat cgttaacacc accttcccgg ctatcttcag
    77041 cgccttccac cgcgtgctga atggcatatt cgcctccagc aatcaaacca accaccagac
    77101 ccggtttcac gccgtaggtg ggcggacatt cgctggcatc cagaatcccc agacgaccgg
    77161 atgttcccgc accgagataa atcagacgac cgccgccgct gacctgggcg tggataacat
    77221 cgatcgccgc ggcgatatcc ggcaggacgc gatcaacggc aagcggtacg gttttatctt
    77281 catcgttgat aatccggcac atttccagcg tcgatacgcg gtcaatttca gccgaggcgg
    77341 tgttcgagcc ttcagtaatc atcttttcta gttgcatcgg tggtgacctt actatttacg
    77401 atcaaataat tgcagatgta tggaatcata tatcccgaat tgacagactt aaaggaatca
    77461 ttgagtctac actcgcagag tgtgaagagc gtcataagaa cttaagcgga atttttgctg
    77521 ccagcccagg agagtggaag atgttgtacc tgacgaaaat acgcaatgcg gaaagtgaat
    77581 ttaccgggaa cgagcaaaaa attgctgact ttctgcgggc cagagtgagc gagctgaaat
    77641 ccgtctcttc acggcagatg gcgaaacagc tcggtatcag ccagtcgagc attgtgaaat
    77701 ttgcccagaa gctcggcgcg caaggattta ctgaattgcg tatggcgtta attggcgaat
    77761 acagtgccag ccgggagaaa acgaatgcca cggcgctgca cctgcacagt tcgattacca
    77821 gtgatgattc gctggaagtg attgcccgca aactgaatcg cgaaaaagag ctggcgctgg
    77881 agcaaacctg tgcgttgttc gactacgcgc ggctgcaaaa aatcattgag gtcatcagta
    77941 aagcaccgtt tatccagata acgggcctgg gcggatcggc gctggtgggg cgcgatctgt
    78001 cgttcaaact gatgaaaatt ggctatcgcg tggcctgcga agccgacacg cacgtacaag
    78061 ccaccgtttc ccaggcgttg aaaaaaggcg atgtacaaat cgctatctct tacagcggca
    78121 gtaaaaaaga gattgtcttg tgcgcagaag cggcacgaaa gcagggggca acggtgattg
    78181 ccattacttc tctggctgac tctccgctgc ggcggctggc gcatttcacg ctcgataccg
    78241 tttccggtga aaccgaatgg cgtagctcgt cgatgtctac ccgcacggcg caaaattctg
    78301 ttaccgattt actgtttgtt ggtcttgtgc aacatcaatg atgtggcgtc attgaaaatg
    78361 attcagcgca gcagtgagct aacacaacgc ctgaaatgat gcataaagca gcgactggat
    78421 tgagattttc ctgaattagt gagctgatcc gcagcaatat tttgtttatc ctgtattttc
    78481 agagggaatg gagtgtaacg ctctgtatta acaaggagag cattaaaatg ggtaaactca
    78541 cgggcaagac agcactgatt acgggcgcat tgcagggaat tggcgaagga attgccagaa
    78601 cttttgcacg tcatggcgcg aacctaatct tgctggatat ctcccctgag atcgaaaagc
    78661 tagcggacga actgtgtggt cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg
    78721 acccggcgtc ggtagccgca gctatcaaac gcgcgaagga aaaagaaggg cgcattgata
    78781 tcctagtgaa taacgcaggc gtttgtcgtc tgggcagttt cctcgatatg agcgatgaag
    78841 atcgcgattt ccatattgat atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc
    78901 tgccggagat gattgcgcgc aaagatggtc gcattgtgat gatgtcttca gtcactggtg
    78961 atatggtggc cgatcctggc gaaacggcgt atgccttaac gaaagcggcg attgttggcc
    79021 tgactaaatc gctggcggtg gagtacgcgc aatccggtat tcgcgttaac gccatctgcc
    79081 cgggatacgt ccgcacgcca atggcggaaa gcattgcccg ccagtcgaac ccggaagatc
    79141 cagaatcggt gctgactgaa atggcaaaag caatcccgct gtgtcgcctc gccgatccgc
    79201 tggaagtcgg cgaactggcg gccttcctcg catcggatga atccagctat ttaaccggta
    79261 cacagaatgt gattgatggc ggcagcacac tgccggagac ggttagcgtc ggtatctgat
    79321 tcacctctgt ttcctccctg catgtgtggg gaggatttcg tcttgaacta agttcaccag
    79381 gctatttcat ttgtcatttt ggccccgggc agtgctcgaa atcctcacgt actatgtgta
    79441 cgctccggtt tctccgcgct gtccgtgtcc aaactgcctg caacaattac gcctgttgaa
    79501 ccaagttctt attccctttt taacttccaa atcaccaaac ggtatataaa accgttactc
    79561 ctttcacgtc cgttataaat atgatggcta ttagaaagtc attaaattta taagggtgcg
    79621 caatggccgt taacttactg aaaaagaact cactcgcgct ggtcgcttct ctgctgctgg
    79681 cgggccatgt acaggcaacg gaactgctga acagttctta tgacgtctcc cgcgagctgt
    79741 ttgccgccct gaatccgccg tttgaacagc aatgggcaaa agataacggc ggcgacaaac
    79801 tgacgataaa acaatctcat gccgggtcat caaaacaggc gctggcgatt ttgcagggct
    79861 taaaagcaga cgttgtcact tataaccagg tgaccgacgt acaaatcctg cacgacaaag
    79921 gcaagctgat cccggctgac tggcagtcgc gcctgccgaa taatagctcg ccgttctact
    79981 ccaccatggg cttcctggtg cgtaagggca acccgaagaa tatccacgac tggaacgacc
    80041 tggtgcgctc cgacgtgaag ctgattttcc cgaacccgaa aacgtcgggt aacgcgcgtt
    80101 atacctatct ggcggcatgg ggcgcagcgg ataaagctga cggtggcgat gaagccaaaa
    80161 ccgaacagtt tatgacccag ttcctgaaaa acgttgaagt gttcgatacc ggcggtcgtg
    80221 gcgcaaccac cacttttgcc gagcgtggct tgggcgatgt gctgattagc tttgaatcgg
    80281 aagtgaacaa catccgtaaa cagtatgaag cacagggctt tgaagtggtg atcccgaaaa
    80341 ccaatattct ggcggaattc ccggtggcgt gggtcgataa aaatgtgcag gccaacggta
    80401 cggaaaaagc cgcaaaagcc tacctgaact ggctctacag cccacaggcg cagaccatca
    80461 tcaccgacta ttactaccgt gtgaataacc cggaagtcat ggacaaactg aaagataaat
    80521 tcccgcagac cgaactgttc cgcgtggaag acaaatttgg ctcatggccg gaagtgatga
    80581 aaacccactt caccagcggc ggcgagttag acaagctgtt agcggcgggg cgtaagtaat
    80641 gtttgcagtt tcttcaagac gcgtgctgcc gggctttact ttaagccttg gcaccagtct
    80701 gctgtttgtg tgcctgatat tgctgctgcc gctctctgcg ctggtgatgc agctttctga
    80761 gatgagctgg gcgcagtact gggaggtgat caccaacccg caggtggtcg cggcctacaa
    80821 agtgacgctg ctgtcggcgt ttgtggcatc gatttttaac ggcgttttcg gtctgctgat
    80881 ggcgtggatt ttaacacgtt atcgctttcc gggccgcacg ctgcttgatg cgctgatgga
    80941 tttacccttt gcgctgccaa cggcagtcgc cggtttaacg ctggcctcgc tcttttccgt
    81001 aaacggtttt tacggtgaat ggctggcgaa gtttgatatc aaagtcacct atacctggct
    81061 tggtattgcg gtggcgatgg cctttaccag cattccgttt gtggtgcgta ccgtgcagcc
    81121 ggtgctggaa gagttaggcc cggaatatga agaagcggcg gaaacgcttg gtgcaacgcg
    81181 ctggcagagt ttctgcaaag tggtgctgcc ggagctttct ccggcgctgg tggcgggcgt
    81241 ggcgctgtcg tttacccgta gtcttggtga atttggcgcg gtgattttta tcgccggaaa
    81301 tatcgcgtgg aagacggaag tgacgtcgct gatgattttt gtgcgcttac aggagtttga
    81361 ttacccggca gcgagcgcga ttgcttcggt gatcctcgcg gcatctctgc tgctgctgtt
    81421 ctcaattaac actctgcaaa gtcgctttgg tcggcgtgtg gtaggtcatt aatggcggaa
    81481 gttacccaat tgaagcgtta tgacgcgcgc ccgattaact ggggcaaatg gtttctgatt
    81541 ggcatcggga tgctggtttc ggcgttcatc ctgctggtgc cgatgattta catcttcgtg
    81601 caggcattca gcaaggggct gatgccggtt ttacagaatc tggccgatcc ggacatgctg
    81661 cacgccatct ggctgacggt gatgatcgcg ctgattgccg taccggtaaa cctggtgttc
    81721 ggcattctgc tggcctggct ggtgacgcgc tttaacttcc cgggacgcca gttactgctg
    81781 acgctactgg acattccgtt tgccgtatcg ccggtggttg ccggtctggt gtatctgctg
    81841 ttctacggct ccaacggccc gctcggcggc tggctggatg aacataatct gcaaattatg
    81901 ttctcctggc ccggaatggt gctggtcacc atctttgtga cgtgtccgtt tgtggtgcgc
    81961 gagctggtgc cggtgatgtt aagccagggc agccaggaag acgaagcggc gattttgctt
    82021 ggcgcgtccg gctggcagat gttccgtcgc gtcacattgc cgaatattcg ctgggcgctg
    82081 ctttacggcg tggtgctgac caacgcccgc gcgattggcg agtttggtgc tgtgtcggtg
    82141 gtttccggct cgattcgcgg cgaaacactt tcgctgccgt tacagattga attgttggag
    82201 caggactaca acaccgtcgg ctcctttacc gccgccgcgc tgttgacgct gatggcgatt
    82261 atcaccctgt ttttaaagag tatgttgcag tggcgcctgg agaatcagga aaaacgcgcg
    82321 cagcaggagg agcatcatga gcattgagat tgccaatatc aagaagtctt ttggtcgcac
    82381 ccaggtgctg aacgatatct cactggatat tccttctggt cagatggtcg cgttgctggg
    82441 gccgtccggt tccgggaaaa ccacgctgct gcggattatc gccgggctgg agcatcaaac
    82501 cagcgggcat attcgcttcc acggcaccga cgtgagtcgc ctgcacgcgc gcgatcgtaa
    82561 agtcggtttc gtgttccagc attacgcgct gttccgccat atgacggtat tcgacaatat
    82621 cacttttggc ctgacggtgc tgccgcgtcg cgagcgcccg aatgccgcag ccatcaaagc
    82681 gaaagtgaca aaattgctgg aaatggtcca gcttgcgcat ctggcggatc gttatccggc
    82741 gcagctttcc ggcggccaga aacagcgtgt ggcgctggcg cgcgcgctgg ctgtagaacc
    82801 gcaaattctg ctgcttgatg aaccgtttgg cgcgctggat gcgcaggtgc gtaaagagct
    82861 gcgtcgctgg ctgcgtcaac tgcatgaaga gctgaaattc accagcgtgt ttgtgaccca
    82921 cgaccaggaa gaggcgaccg aagtagccga tcgtgtagtg gtgatgagtc aggggaatat
    82981 tgaacaggct gatgcgccga atcaggtatg gcgcgaacca gcgacccgtt ttgtactcga
    83041 atttatgggc gaagtgaacc gcctgcaggg aaccattcgc ggcgggcagt tccatgttgg
    83101 cgcgcatcgc tggccgctgg gctacacacc tgcgtatcag ggtccggtgg atctcttcct
    83161 gcgcccgtgg gaagtggata tcagccgccg taccagtctc gattcgccgc tgccggtaca
    83221 ggtactggaa gccagcccga aaggtcacta cacccaatta gtggtgcagc cgctggggtg
    83281 gtacaacgaa ccgctgacgg tcgtgatgca tggcgacgat gccccgcagc gtggcgagcg
    83341 tttattcgtt ggtctgcaac atgcgcggct gtataacggc gacgagcgta ttgaaacccg
    83401 cgatgaggaa cttgctctcg cacaaagcgc ctgataggtt gagtgaatgt taaacgcccg
    83461 gaggcgcttc ccgcgatccg ggctttttaa tggcaaggtt tgtaacctgt aggcctgata
    83521 agacgcgcaa gcgtcgcatc aggcaacacc acgtatggat agagatcgtg agtacattag
    83581 aacaaacaat aggcaatacg cctctggtga agttgcagcg aatggggccg gataacggca
    83641 gtgaagtgtg gttaaaactg gaaggcaata acccggcagg ttcggtgaaa gatcgtgcgg
    83701 cactttcgat gatcgtcgag gcggaaaagc gcggggaaat taaaccgggt gatgtcttaa
    83761 tcgaagccac cagtggtaac accggcattg cgctggcaat gattgccgcg ctgaaaggct
    83821 atcgcatgaa attgctgatg cccgacaaca tgagccagga acgccgtgcg gcgatgcatg
    83881 cttatggtgc ggaactgatt cttgtcacca aagagcaggg catggaaggt gcgcgcgatc
    83941 tggcgctgga gatggcgaat cgtggcgaag gaaagctgct cgatcagttc aataatcccg
    84001 ataaccctta tgcgcattac accaccaccg ggccggaaat ctggcagcaa accggcgggc
    84061 gcatcactca ttttgtctcc agcatgggga cgaccggcac tatcaccggc gtttcacgct
    84121 ttatgcgcga acaatccaaa ccggtgacca ttgttggcct gcaaccggaa gaaggcagca
    84181 gtattccggg cattcgccgc tggcctgcgg aatatctgcc ggggattttc aacgctgcgc
    84241 tggtggatga ggtgctggat attcatcagc gcgatgcgga aaacaccatg cgcgaactgg
    84301 cggtgcggga aggaatattc tgtggcgtca gctcaggcgg cgcggttgct ggagcactgc
    84361 gggtggcaaa agctaaccct ggtgcggtgg tggtagcgat catctgcgat cgtggcgatc
    84421 gctacctctc caccggagtg tttggggaag agcattttag tcagggggca gggatttaag
    84481 gattaatagc attggagact gatgacaaac gcaaaactgc ctgatgcgct ccgcttatca
    84541 ggcctacaat gtttctgcaa tatattgaat tagcaatatt tttatgccgg atcaggcgtt
    84601 tacgccgcat ccggcataaa caaagtgcac tttgtcaata atctgatttg tcggcagttg
    84661 ttgctgccga caaatgtagt ttttaatttt cgtgtggaaa agaagtaagg tcatcaatca
    84721 ttttttgaag tattggtgag tcctgaccgt caccccattg aagaattttt gcggtaagct
    84781 gatgacgcgc tagtgcattg agtcccatct ttttaagttt atgggagatt ttacccatga
    84841 ctttgatctg accgaatgca gtcgcgtagg taatcatatt gctctggata gagctcattt
    84901 catcggctag taattgcgca cgcacatcct cttcaagaat actgtccata gcgatgtatt
    84961 tcatacccca catagtttca attaacattt gagggaaatc agcacagtcc agaacggagg
    85021 ctattttcaa gttcgtttag agcatcagca ccttcatcgc taccaaaagg agattcttca
    85081 tcccagcaac acatataaag tggatcagta aaatggcact ggaagtttgg gtgtgacgtt
    85141 ttaagatgta acccaatctc ttcatcatca aaatagtaga gatccatgaa gttaaagtct
    85201 ggagtttctt gatatccttt tttcattttt gcggcaatca atttactggc ttctttcaga
    85261 cactgttctt cattatcgaa ttcttttgta tggaatttac caatactacc taccttgcca
    85321 tagttgacag ctaaactatc cccttcgtaa tctatccacc aaaatttatt cgatttttca
    85381 tcgtgataaa taaagcattt tttcatgtta cctcctgtaa atgccttacc aaagcggatt
    85441 aatttccttc cgcaggcacc gttttatccg tctgcgcaat taacgcatgt aaaaaaggct
    85501 gtgcgttctc atcgctctta ccggggatct tcacgatata cggcttcccg gtaatcgatg
    85561 acgacgaagc taccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc
    85621 cgagcttcag acgcaggtgg gaaaccgctt catcgcaggt atgttcatca ccgttgcgca
    85681 caaatatcaa atcctttttc tgtgctaatc cctccagcat ggcgttgatg cgggcttctt
    85741 catgaacagt taacttcgcg taagcgggga gtgtcattag cagcgtaatg gccagacaaa
    85801 tgattttctt cactgaatta ccttatcttt gttttgtaat agccgatttg actgccagaa
    85861 tcaggtaaag tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg
    85921 agtagtttgt tgttgtttaa cgataagagt agggcgctgc aggcggatat cgtcgccgtg
    85981 cagtcgcagg tagtgtacgg aagcgtaggc aacagcattg cggtgcctgc tataaaacag
    86041 aacggcttga atgtctttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac
    86101 actttctacg gtggtgcgat tccggacgaa tggtttagcg gctatttgcg tgcgcttcag
    86161 gagcgtgatg cgctgcgcca acttcgtgct gtaaccacgg gctatatggg aacggcatcg
    86221 caaatcaaaa tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg
    86281 atcatggtcg atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt
    86341 cccgaggcgt atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt
    86401 gagctggaaa tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca
    86461 aaaagtctgc tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa
    86521 gaaaatcagg agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat
    86581 tcacgggtaa aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt
    86641 ggtttgctga aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg
    86701 gaagtgatgc gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg
    86761 gaagcataaa aaatggcgcc gatgggcgcc atttttcact gcggcaagaa ttacttcttg
    86821 atgcggataa ccggggtttc acccacggtt acgctaccgg acagtttgat cagttctttg
    86881 atttcgtcca tgttggagat aacaaccgga gtcagggtag acttggcttt ctcttccagc
    86941 agcggcagat caaattcaat gacagtatcg ccaactttca cgcgctgacc ttcttcagca
    87001 atacgcttga agccttcgcc tttcagttca acggtgtcga taccgaagtg gacgaacagt
    87061 tcaacgccgc tatcagattc gatagagaac gcgtgattgg tttcaaagat tttaccaatg
    87121 gtaccgtcta caggcgcgac cattttgtta cccgttggtt tgatagcaat accatcacca
    87181 acgatttttt ccgcaaaaac gacatccggc acgtcttcga tattgacgat ctcgccagag
    87241 agcggagcaa tgatctcaat agttccggta tcctttttgt cgtcggaaac cagagatttc
    87301 agtttatcga acaaacccat gatcttctcc taagcagtaa attgggccgc atctcgtgga
    87361 ttagcagatt gttttttctt caatgaactt gttaaccagc gtcattaact cgtccgttgt
    87421 cggttgagca agagcctgct ctgctaacac cttcgcatct tcgaagttcg tgttacggat
    87481 aatcttctta atgcgcggga tagaaatggc gctcatagag aattcgtcca gacccatccc
    87541 cagcaacaga agtgtagcac gttcatcgcc agcaagctca ccacacatgc cagtccattt
    87601 gccttcagca tgagaagcat caataacttg cttgatcaag ttcagcacgg acggtgacat
    87661 tggctggtaa aggtgtgaaa tcatatcatt accacggtca actgccagag tgtactgcgt
    87721 taaatcattg gtgccgatac taaagaaatc aacttctttg gctaaatgac gtgcaattgt
    87781 tgcggcagcc ggtgtttcca ccattacgcc gatttcaatt gactcgtcaa acgctttacc
    87841 ttcgtcgcgc agttcctgtt tgtagatttc gatctctttg cgcagtgcac gcacttcttc
    87901 aacagagatg atcatcggga acataatgcg caatttaccg aaagccgagg cacgcaggat
    87961 agcgcggagc tgatcgcgca ggatctcttt acgatccatc gcgatacgga tagcgcgcca
    88021 gccgaggaac gggttctctt ctttcgggaa gttcatgtat ggcagctctt tgtcgccgcc
    88081 gatgtccatg gtacgaacga taaccgcctg cgagccacac gcttcagcca ctgctttgta
    88141 agcagcaaac tgttcttctt cagtgggcag cgcgtcgcgg tccatgaaca ggaactcagt
    88201 acgatacaga ccaacgcctt cagcgccgtt acgctctgca ccttcaacgt cacgaaccgt
    88261 accaatgtta gcgcatactt ctacctggtg accgtccagc gtaatagctg gcagatcttt
    88321 cagtttagca agctctgctt tttcagaagc cacttgctcc tgaacagcgc gcattttatc
    88381 aataacttcg ttggttggat tgacgtaaac ctgattattt acggcatcca gaatcagata
    88441 gtcgtcattt ttcacctgag aggtgacgct accggtaccc acgatagcag gcagttccag
    88501 agaacgcgcc atgatagagg tgtgggaagt acggccaccc gcgtcggtga tgaaacccag
    88561 caccttcttc aggttcagct gtgcggtttc ggacggcgtc aggtcagcgg caaccagaat
    88621 gacttcatcc tgaatggcgc tcaggtcgat aatcttcagg cccaggatgt tgcgcagcag
    88681 gcgcttaccg atatcacgta cgtcagccgc acgttctttc aggtattcat catccagctc
    88741 ttccagggca gaagcctgac cttcgataac ttcatgagca gctgcgtcag ctgtcatgtg
    88801 tttatcttta atcagggcta tgatttcctg ctccagctcc tcatcttcga gcagcataat
    88861 atgcccttca aagatggctt ctttttcttc accgaacgtt tcaccagctt tcgttttgat
    88921 cgtttccagc tgggctgatg ccttggcacg accgctcaga aaacgttcaa cttcctgagc
    88981 aacctggtcg gcagaaattt ttttccggtc aatgacaatt tcgtcttctt tcagaagcag
    89041 agctttaccg aaagcgatac ccggggatgc taaaatgcct gaaatcataa ccctacctta
    89101 cttgtgactg atttttaaaa gaacccggga aattactcga gttccgccat cagtttaacc
    89161 agatgttcaa ccgctttctg ctcgtcttcg ccttctgcgg agatagtcac aacggtacct
    89221 tgagtcaggc ccagagtctg cagtttaaac aggcttttcg cgctggcgct tttgccgttg
    89281 gaagtcacag taatttcaga agtgaagccc ttagcttctt ttacaaactg ggcagcaggg
    89341 cgggtgtgca gaccgttcgg agcggtaatg gtaacttctt gctggaacat tgtatttccc
    89401 caacttatag gtttagtgtt gtggaactaa agtctagcct ggcggttcga ctttagcctg
    89461 tattgttagc gccagttttt aacagacgcg acgcacgaag agcgcaatac agtccattgc
    89521 tggcgggtgt tgaccgctga cgtttcgtac gtcattaaac attatgccgc taaaggaaga
    89581 attgaaccaa atcataaaat cgattcagct ttggcttttc ctgtaacgat taatttcgcg
    89641 catcaaaata attagtctgg ttaaatacca gttccgacag gctgaatcaa tgccaggagg
    89701 ggcgaaagtt tgaagcaggc cacaaaaaag cacctaaaaa ggtgcttttt tacgcatttt
    89761 taacaagctg gcattactgt tgcaattctt tctcagtgaa gagatcggca aacaatgcgg
    89821 tgcttaaata acgctcaccc gatgatggta gaataaccac aatattcttg ttggtaaagc
    89881 tttcatcttc ttgtagtttc aacgctgcgg caacagctgc tccagaagag atacctgcaa
    89941 gaataccttc ttcttccatt agacgacggg cggtagaaat ggcttcttca ttggtgatgc
    90001 caatgacttt atcgaccagc ttgagatcga ggttagccgg gataaaacca gcgccaatac
    90061 cctgaatttt atgcgggcca ggtttaatct cttcacctgc cagcacctgg gcgataactg
    90121 gagaatcggt tggctcaacg gcgacagaga taagatcggt cttgcctttg gtgcctttaa
    90181 tgtagcggct gacgccagtc agcgtaccgc cagtcccaac gccagcaata aatacatcaa
    90241 cctgaccgtc ggtatcttcc cagatctccg gaccggtggt tttttcgtga atttcagggt
    90301 ttgccggatt gctgaattgt tgcagcagca ggtatttctc tggattgctg gcgacaattt
    90361 cttctgcttt ttggattgcg cctttcatgc ctttagcacc ttccgtcagc accaggtttg
    90421 cacctaacgc tttcagcagc ttgcggcgtt caatactcat ggtttctggc atggtcaggg
    90481 tgagtttgta accgcgagcg gcagctacat aggccagtgc aatcccggta ttaccgctgg
    90541 tcggttcaac cagttcaacg cctggtttca gcacgccgcg cttttcggca tcccaaatca
    90601 tgttggcacc gatacggcac ttaacgctga agctggggtt acgagattcc accttcgcca
    90661 gaatgcgtcc gttaccgatg cgattcaggc gaaccagcgg cgtgtgaccg atagtcagcg
    90721 agttatcttc aaaaatctta ctcatggcct gtccttaact gtatgaaatt gggatacaac
    90781 aggtagcata cccgctcaga gaatatgcgg aagtaaggat ttagcatatc tatatacaga
    90841 agggaaataa tgacatgcaa gatggaataa ggggcggcat aagccacccc tgtttcacac
    90901 aaacggttta caaaataacc ggtagattgt taccgccaca tcgcgtgttt atcgcgatag
    90961 caatcgaccc acatcgccgt cgcgccacaa acggcaacgg gcatgatgaa cagattaagc
    91021 agcgggatca tcgtaaacag gctggttaaa gcaccaaact gcatattggt gattttgcgt
    91081 gtgcgcaggg cggtgcgcat ctctttaaac ggcactttgt ggttatcgaa ggggtaatcg
    91141 caatactgga tggctaacat ccaggcgcta aacaggaacc acagtaccgg cgcgacggtt
    91201 tgcccaatac cagggatgaa gtaaagaatt agtaatacaa ttgcgcgcgg cagataccag
    91261 acaaattttt gccattcgcg tttcatgatt cgcggcacat ctttcatgat accgaaaatc
    91321 ccggtatctg gcggtgtagc gccagtcaat cgtgcttcca gttgttcagc caataaaccg
    91381 ttaaacggag cggcaatcca gttagcaatc gtggagaaga aatagccaaa cactaacagc
    91441 acagagatga cagccagagg ccacaacaga taactcagcc attgtagcca gtccggaacg
    91501 taactcatga aagtcgggat ccagacatcg agctgtgtaa agagccacca gaatgcgccc
    91561 cccatcaaca aaatattgac cagcagcggt aaaataacga aacgccgaat cccaggttgc
    91621 gagacgagct tccagccttg cgcaaaatag taaaaaccgc tgcgtggggc agatgtgaat
    91681 gatgaaacca taatcaggat gagctccttt tgaccaatcc caggaaaatt ctgcgtattt
    91741 taccgggtaa ttgcgcaatg gacagttagg atatgttcga aaaaacagca aaaagcacga
    91801 tttcatctat ctttgtgctg tgaaagttaa tagtgcactt gcacttgagg taatcggcaa
    91861 atactcttag tgagtaaatg tttgccgtgg tggcaaggtg ttagaacaac agagaatata
    91921 atgatgcagg atttgcgtct gatattaatc attgttggcg cgatcgccat aatcgcttta
    91981 ctggtacatg gtttctggac cagccgtaaa gaacgatctt ctatgttccg cgatcggcca
    92041 ttaaaacgaa tgaagtcaaa acgtgacgac gattcttatg acgaggatgt cgaagatgat
    92101 gagggcgttg gtgaggttcg tgttcaccgc gtgaatcatg ccccggctaa cgctcaggag
    92161 catgaggctg ctcgtccgtc gccgcaacac cagtaccaac cgccttatgc gtctgcgcag
    92221 ccgcgtcaac cggtccagca gccagtttcg ccacaggtcg cgccagcgcc gcagccagtg
    92281 cattcagcac cgcaaccggc acaacaggct ttccagcctg cagaacccgt agcggcacca
    92341 cagcctgagc ctgtagcgga accggctcca gttatggata aaccgaagcg caaagaagcg
    92401 gtgattatca tgaacgtcgc ggcgcatcac ggtagcgagc taaacggtga actgcttctt
    92461 aacagcattc aacaagcggg cttcattttt ggcgatatga atatttacca tcgtcatctt
    92521 agcccggatg gcagcggccc ggcgttattc agcctggcga atatggtgaa accgggaacc
    92581 tttgatcctg aaatgaagga tttcactact ccgggtgtca ctatctttat gcaggtaccg
    92641 tcttacggtg acgagctgca gaacttcaag ctgatgctgc aatctgcgca gcatattgcc
    92701 gatgaagtgg gcggtgtcgt gcttgacgat cagcgcagta tgatgactcc gcagaaattg
    92761 cgcgagtacc aggacatcat ccgcgaagtc aaagacgcca acgcctgata cacttaaggc
    92821 aaattaactc ctcttcgaac ccccgcttgt cgggggtttt tagcattgat ggtgcgatat
    92881 ggaatcaatc gaacaacaac tgacagaact gcgaacgacg ctttgccatc atgaatatct
    92941 ttatcatgtg atggatgcgc cggaaattcc cgacgctgaa tacgacaggc tgatgcgcga
    93001 actgcgcgag ctggaaacca aacatccaga actgattacg cctgattcgc ctacccaacg
    93061 tgtaggtgct gcgccgctgg cagctttcag ccagatacgc catgaagtac caatgctgtc
    93121 actggataac gtttttgatg aagaaagctt tcttgctttc aacaaacgtg tgcaggaccg
    93181 tctgaaaaac aacgagaaag tcacctggtg ctgtgagctg aagctggatg gccttgccgt
    93241 cagtattctg tatgaaaatg gcgttttagt cagtgccgcg acccgtggcg atggcaccac
    93301 cggggaagat atcacgtcta atgtgcgtac tattcgcgcc attccgctga agctgcacgg
    93361 agagaatatc ccggcgcgtc tggaagtgcg tggtgaagtg ttcctgccgc aggcggggtt
    93421 cgaaaagatt aacgaagatg cgcgacgcac gggcgggaaa gtttttgcta acccacgtaa
    93481 tgcggcagct ggttcactgc gtcagcttga tccgcgtatt acagcgaagc gaccgctcac
    93541 ttttttctgc tatggcgttg gtgttctgga agggggcgag ctgccggata ctcatcttgg
    93601 ccgtttactg caatttaaaa agtgggggtt gccggtcagc gatcgggtaa cgctttgtga
    93661 atcggcggaa gaagtgctgg cgttctatca caaagtggaa gaagaccgcc cgacgctggg
    93721 ctttgatatc gacggcgtgg tgattaaggt caactcactg gaacagcagg agcagcttgg
    93781 ctttgtcgcg cgtgccccgc gctgggcggt agcgtttaaa ttcccggcgc aggagcagat
    93841 gacctttgtg cgtgacgtcg agtttcaggt tgggcgtact ggcgcgatta cgcctgttgc
    93901 gcgtctggaa cctgtccatg ttgctggcgt gctggtgagt aacgcaacct tacacaatgc
    93961 ggatgaaatc gaacgtcttg gtttacgcat tggcgataaa gtggtgattc gccgcgctgg
    94021 cgacgtgatc ccgcaggtgg ttaacgtcgt gctttctgaa cgcccggaag atacccgtga
    94081 ggttgtattc ccgacgcatt gtccggtatg tggttctgac gttgagcgtg tggaaggtga
    94141 agcggttgcc cgctgtaccg gtggcctgat ttgcggtgcg cagcgtaaag agtcgctgaa
    94201 acactttgtt tcccgccgtg cgatggatgt tgacggaatg ggcgacaaaa tcatcgatca
    94261 gctggttgaa aaagaatatg tccatactcc ggcagatctg ttcaaactca ccgcaggcaa
    94321 actgaccgga ctggagcgta tggggccaaa attggcacaa aacgtggtta acgcgctgga
    94381 aaaagcgaaa gaaaccacct ttgctcgctt cctctatgca cttggcatcc gtgaagtcgg
    94441 cgaggccacc gcagcaggtc tggcggcata tttcggcacg ctggaagcgc tggaagccgc
    94501 ttcgattgaa gagctgcaaa aggtgcctga tgttggcatt gtcgttgcat cccacgttca
    94561 caacttcttt gccgaagaaa gcaaccgcaa tgtcatcagc gagctgttgg cggaaggtgt
    94621 tcactggcct gcgccgatcg ttatcaacgc ggaagagatt gacagcccgt ttgctggtaa
    94681 aaccgtggtg cttacgggca gcttaagcca gatgtcgcgt gatgacgcta aagctcgact
    94741 ggtcgaactg ggcgcgaaag tcgcgggcag cgtgtcgaag aaaaccgatc tggtgatagc
    94801 gggtgaagct gcaggatcta aactggcgaa ggcgcaggaa ctgggcattg aagtcatcga
    94861 cgaagcggaa atgctgcgtt tgctgggtag ctgagatgga aaaagagcag ctgattgaaa
    94921 tagccaatac gataatgccg tttggcaaat acaaagggcg tcgcttaatt gacctgccgg
    94981 aggagtatct gctgtggttt gcccgcaaag atgagttccc ggcagggaag ctcggtgagc
    95041 taatgcaaat cacgctgctg attaaaaccg aggggctgac gcaactggtc cagcccctga
    95101 agcgtccgct ttaagcttta tcggcgctgc tttcctgctg cgcctgtaac tgttcggtct
    95161 ggcgtttgta tcgacgcgct agcaccgcac agaccatcaa ttggatctga tggaaaatca
    95221 tcaggggcag caccatcata ccgatcaccg atgtggggaa cagaatgttt gccatcggga
    95281 tgccatttgc cagactcttt ttcgaaccac aaaagacgat agtaatttca tctgccttat
    95341 tgaagcccag tcggcgtgcc ataaagacgt taactacaat cacgatagcc agaagaacgc
    95401 agctgaccac cacgataaac agcaatgatc cccagccaac tttatgccag ataccattaa
    95461 cgacggcttc gctgaacgct gtataaacca ccaacagaat ggacgtctgg tcagttttcg
    95521 caatccattt tttattgcgc gacacccagt caccaatcca cggccgggaa agatgcccca
    95581 acacaaacgg cagcagcagt tgcagcataa ttttaccgac ctgctcaagg ctgccccttg
    95641 caccgtgaac gttcatcacc agaccaacca gcaatggtga aaggaaaatc cccagcaggc
    95701 tggatgctga cgcagaacaa accgccgccg cgacgttacc gcccgccatt gacgtgaagg
    95761 cgattgcaga ctgcacggta gccgggagaa tgcacaagta gagaaaaccg gagtagagca
    95821 tcgggtcgac atttaccggt ttccaccagg caaacagtac acccagaatc ggaaacagca
    95881 cgaaggtgct gcacattacc cacaaatgca gtcgccagtg accaccgcca gcaataatcg
    95941 cctcacgcga caacttcgcg ccgtgcataa agaacagcag ggcaatagct gcggtggtca
    96001 gattttcaaa gaaggggacg aaatcgcctc tggccggaaa gaaagaggcc agcaacacca
    96061 ccgtgatcag ggttaaggtg aaaggatcga ggatacgaaa aagtttcata aagactccag
    96121 atgatcgatg gttctattgt gcttcttcca gtttgagaaa taaaattgat ttattgcatc
    96181 cattcatgaa gaaaacagat gaattattct ttaaaacaat taaaggtttt cgtcacagta
    96241 gcgcaggaga aaagttttag tcgtgcagga gagcgtatcg gcttgagcca gtcggcagta
    96301 agtcacagtg tgaaggagct ggaaaatcat actggtgttc gcctgctgga cagaaccacg
    96361 cgtgaagtgg tgcttacaga tgcagggcag cagttggctt tgcgtcttga gcgactgctg
    96421 gatgaactta acagcacgtt gcgcgatacc gggcgtatgg ggcaacaact gagcggaaaa
    96481 gttcgggtcg ctgccagcca gaccatttcc gcgcatctta ttccgcaatg cattgctgaa
    96541 agccatcgcc gctatccaga tattcagttt gtcctgcatg atcgtccgca gcagtgggtg
    96601 atggaaagta ttcgccaggg agatgtcgac tttggcatcg tcatcgatcc aggcccagtg
    96661 ggcgatctgc aatgtgaagc gattctttcc gagcctttct ttctgctttg ccatcgcgat
    96721 agcgctttgg ccgtggaaga ttatgtaccc tggcaggcat tacagggagc gaaattggtg
    96781 ttgcaggatt acgcgtcagg cagccgaccg ctgattgacg cagcgctggc gcgcaatggt
    96841 attcaggcga atattgtgca ggagattgga catccggcga cgctgttccc gatggtagcc
    96901 gcaggcatcg gcatcagtat tcttcctgca ctggcgctac cattgcctga aggtagccca
    96961 ctggtggtta agcgcattac gccggtggtt gaacgccagt tgatgctggt gcgcaggaag
    97021 aaccgttcac tttccaccgc cgctgaagcg ttatgggacg tggtacgaga ccagggcaat
    97081 gcgctaatgg ctggtcggga aggggatccg ttgtatcaga tatagatatc gatatgagtc
    97141 gttttttcaa caggatttag aagggataca ttcagagaca aacgctgctc tttctcctgc
    97201 gccttttctg cctgctgttt ctgcaactgc gccagttgtg tttccagtag ggtgatttgc
    97261 tgttgaatca attcggcctg tttttgcttg tcttcagcac tgccgctagt gtctacaatc
    97321 tctttaattt gttgagtgag tttgatgatt tgctgactta tccgcgcgat ttgtgaagca
    97381 acatcgctac cttgtgatgc cctgttacta ctctgtgttt gtatggaagg aattgaagtt
    97441 gaagagatag tggccattgt tcagtcctcg tcactgtacg aggtaatatc ggtagaagaa
    97501 caaggaactt gagaagaaaa atcaatctta caaatcgcag atagcaaaaa agcgccttta
    97561 gggcgctttt ttgcattggt gggtcgtgca ggatgactcg cttcgctcgc ccttcgggcc
    97621 gtcgccgcga gcggctccgt tgtttcgctg cgctcaactc aaacctgctg caggttcgaa
    97681 tctttgagta tcgcagatag
//
LOCUS       DAJTYI010000003        68382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYI010000003 DAJTYI010000000
VERSION     DAJTYI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..471)
                     /gene="rsmI"
                     /locus_tag="OO564_000201"
     CDS             complement(<1..471)
                     /gene="rsmI"
                     /locus_tag="OO564_000201"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6651469.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALK"
     gene            536..2572
                     /gene="lpoA"
                     /locus_tag="OO564_000202"
     CDS             536..2572
                     /gene="lpoA"
                     /locus_tag="OO564_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6651470.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            2530..2925
                     /locus_tag="OO564_000203"
     CDS             2530..2925
                     /locus_tag="OO564_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6651471.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            2945..3535
                     /gene="diaA"
                     /locus_tag="OO564_000204"
     CDS             2945..3535
                     /gene="diaA"
                     /locus_tag="OO564_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6651472.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            3545..4120
                     /gene="yraP"
                     /locus_tag="OO564_000205"
     CDS             3545..4120
                     /gene="yraP"
                     /locus_tag="OO564_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6651473.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4234..5274)
                     /locus_tag="OO564_000206"
     CDS             complement(4234..5274)
                     /locus_tag="OO564_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6651474.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5347..5982)
                     /locus_tag="OO564_000207"
     CDS             complement(5347..5982)
                     /locus_tag="OO564_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6651475.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSFSELRKRAE"
     gene            6110..6628
                     /gene="yhbO"
                     /locus_tag="OO564_000208"
     CDS             6110..6628
                     /gene="yhbO"
                     /locus_tag="OO564_000208"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6651476.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(6608..7051)
                     /locus_tag="OO564_000209"
     CDS             complement(6608..7051)
                     /locus_tag="OO564_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6651477.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDLGTEQA"
     gene            7102..7404
                     /gene="yhbQ"
                     /locus_tag="OO564_000210"
     CDS             7102..7404
                     /gene="yhbQ"
                     /locus_tag="OO564_000210"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6651478.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(7391..7894)
                     /locus_tag="OO564_000211"
     CDS             complement(7391..7894)
                     /locus_tag="OO564_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6651479.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(7888..8412)
                     /locus_tag="OO564_000212"
     CDS             complement(7888..8412)
                     /locus_tag="OO564_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6651480.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            8621..9616
                     /locus_tag="OO564_000213"
     CDS             8621..9616
                     /locus_tag="OO564_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6651481.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            9625..10503
                     /locus_tag="OO564_000214"
     CDS             9625..10503
                     /locus_tag="OO564_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6651482.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            10584..11591
                     /locus_tag="OO564_000215"
     CDS             10584..11591
                     /locus_tag="OO564_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6651483.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(11709..12953)
                     /gene="mtr"
                     /locus_tag="OO564_000216"
     CDS             complement(11709..12953)
                     /gene="mtr"
                     /locus_tag="OO564_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6651484.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13107..14996)
                     /gene="deaD"
                     /locus_tag="OO564_000217"
     CDS             complement(13107..14996)
                     /gene="deaD"
                     /locus_tag="OO564_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6651485.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(14989..15069)
                     /gene="yrbN"
                     /locus_tag="OO564_000218"
     CDS             complement(14989..15069)
                     /gene="yrbN"
                     /locus_tag="OO564_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6651486.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15176..16060)
                     /gene="nlpI"
                     /locus_tag="OO564_000219"
     CDS             complement(15176..16060)
                     /gene="nlpI"
                     /locus_tag="OO564_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6651487.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16169..18304)
                     /gene="pnp"
                     /locus_tag="OO564_000220"
     CDS             complement(16169..18304)
                     /gene="pnp"
                     /locus_tag="OO564_000220"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6651488.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(18551..18820)
                     /gene="rpsO"
                     /locus_tag="OO564_000221"
     CDS             complement(18551..18820)
                     /gene="rpsO"
                     /locus_tag="OO564_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6651489.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(18969..19913)
                     /gene="truB"
                     /locus_tag="OO564_000222"
     CDS             complement(18969..19913)
                     /gene="truB"
                     /locus_tag="OO564_000222"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6651490.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(19913..20314)
                     /gene="rbfA"
                     /locus_tag="OO564_000223"
     CDS             complement(19913..20314)
                     /gene="rbfA"
                     /locus_tag="OO564_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6651491.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(20478..23126)
                     /gene="infB"
                     /locus_tag="OO564_000224"
     CDS             complement(20478..23126)
                     /gene="infB"
                     /locus_tag="OO564_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6651492.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23151..24638)
                     /gene="nusA"
                     /locus_tag="OO564_000225"
     CDS             complement(23151..24638)
                     /gene="nusA"
                     /locus_tag="OO564_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6651493.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(24666..25118)
                     /gene="rimP"
                     /locus_tag="OO564_000226"
     CDS             complement(24666..25118)
                     /gene="rimP"
                     /locus_tag="OO564_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6651494.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25325..25401)
                     /locus_tag="OO564_000227"
     tRNA            complement(25325..25401)
                     /locus_tag="OO564_000227"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25365..25367),aa:Met,seq:cat)
     gene            25749..27092
                     /gene="argG"
                     /locus_tag="OO564_000228"
     CDS             25749..27092
                     /gene="argG"
                     /locus_tag="OO564_000228"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6651495.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27100..28725)
                     /locus_tag="OO564_000229"
                     /pseudo
     CDS             complement(27100..28725)
                     /locus_tag="OO564_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29184..29270)
                     /locus_tag="OO564_000230"
     tRNA            complement(29184..29270)
                     /locus_tag="OO564_000230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29234..29236),aa:Leu,seq:gag)
     gene            complement(29285..29617)
                     /gene="secG"
                     /locus_tag="OO564_000231"
     CDS             complement(29285..29617)
                     /gene="secG"
                     /locus_tag="OO564_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6651496.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(29845..31182)
                     /gene="glmM"
                     /locus_tag="OO564_000232"
     CDS             complement(29845..31182)
                     /gene="glmM"
                     /locus_tag="OO564_000232"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6651497.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31175..32023)
                     /gene="folP"
                     /locus_tag="OO564_000233"
     CDS             complement(31175..32023)
                     /gene="folP"
                     /locus_tag="OO564_000233"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6651498.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32113..34047)
                     /gene="ftsH"
                     /locus_tag="OO564_000234"
     CDS             complement(32113..34047)
                     /gene="ftsH"
                     /locus_tag="OO564_000234"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6651499.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34147..34776)
                     /gene="rlmE"
                     /locus_tag="OO564_000235"
     CDS             complement(34147..34776)
                     /gene="rlmE"
                     /locus_tag="OO564_000235"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6651500.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34902..35195
                     /gene="yhbY"
                     /locus_tag="OO564_000236"
     CDS             34902..35195
                     /gene="yhbY"
                     /locus_tag="OO564_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6651501.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35351..35827)
                     /gene="greA"
                     /locus_tag="OO564_000237"
     CDS             complement(35351..35827)
                     /gene="greA"
                     /locus_tag="OO564_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6651502.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36075..37508
                     /gene="dacB"
                     /locus_tag="OO564_000238"
     CDS             36075..37508
                     /gene="dacB"
                     /locus_tag="OO564_000238"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6651503.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(37694..38866)
                     /gene="cgtA"
                     /locus_tag="OO564_000239"
     CDS             complement(37694..38866)
                     /gene="cgtA"
                     /locus_tag="OO564_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6651504.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(38882..39847)
                     /locus_tag="OO564_000240"
     CDS             complement(38882..39847)
                     /locus_tag="OO564_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6651505.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(39974..40231)
                     /gene="rpmA"
                     /locus_tag="OO564_000241"
     CDS             complement(39974..40231)
                     /gene="rpmA"
                     /locus_tag="OO564_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6651506.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40252..40563)
                     /gene="rplU"
                     /locus_tag="OO564_000242"
     CDS             complement(40252..40563)
                     /gene="rplU"
                     /locus_tag="OO564_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6651507.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            40822..41793
                     /gene="ispB"
                     /locus_tag="OO564_000243"
     CDS             40822..41793
                     /gene="ispB"
                     /locus_tag="OO564_000243"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6651508.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42021..42299
                     /gene="sfsB"
                     /locus_tag="OO564_000244"
     CDS             42021..42299
                     /gene="sfsB"
                     /locus_tag="OO564_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6651509.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42347..43606)
                     /gene="murA"
                     /locus_tag="OO564_000245"
     CDS             complement(42347..43606)
                     /gene="murA"
                     /locus_tag="OO564_000245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6651510.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(43661..43915)
                     /gene="ibaG"
                     /locus_tag="OO564_000246"
     CDS             complement(43661..43915)
                     /gene="ibaG"
                     /locus_tag="OO564_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6651511.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44075..44368)
                     /gene="mlaB"
                     /locus_tag="OO564_000247"
     CDS             complement(44075..44368)
                     /gene="mlaB"
                     /locus_tag="OO564_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6651512.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44368..45003)
                     /gene="mlaC"
                     /locus_tag="OO564_000248"
     CDS             complement(44368..45003)
                     /gene="mlaC"
                     /locus_tag="OO564_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6651513.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45022..45573)
                     /gene="mlaD"
                     /locus_tag="OO564_000249"
     CDS             complement(45022..45573)
                     /gene="mlaD"
                     /locus_tag="OO564_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6651514.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(45578..46360)
                     /gene="mlaE"
                     /locus_tag="OO564_000250"
     CDS             complement(45578..46360)
                     /gene="mlaE"
                     /locus_tag="OO564_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6651515.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46368..47177)
                     /gene="mlaF"
                     /locus_tag="OO564_000251"
     CDS             complement(46368..47177)
                     /gene="mlaF"
                     /locus_tag="OO564_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6651516.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            47387..48352
                     /locus_tag="OO564_000252"
     CDS             47387..48352
                     /locus_tag="OO564_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6651517.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48366..49352
                     /gene="kdsD"
                     /locus_tag="OO564_000253"
     CDS             48366..49352
                     /gene="kdsD"
                     /locus_tag="OO564_000253"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6651518.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49373..49939
                     /gene="kdsC"
                     /locus_tag="OO564_000254"
     CDS             49373..49939
                     /gene="kdsC"
                     /locus_tag="OO564_000254"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6651519.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            49936..50511
                     /gene="lptC"
                     /locus_tag="OO564_000255"
     CDS             49936..50511
                     /gene="lptC"
                     /locus_tag="OO564_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6651520.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            50480..51037
                     /gene="lptA"
                     /locus_tag="OO564_000256"
     CDS             50480..51037
                     /gene="lptA"
                     /locus_tag="OO564_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6651521.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51044..51769
                     /gene="lptB"
                     /locus_tag="OO564_000257"
     CDS             51044..51769
                     /gene="lptB"
                     /locus_tag="OO564_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6651522.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            51817..53250
                     /gene="rpoN"
                     /locus_tag="OO564_000258"
     CDS             51817..53250
                     /gene="rpoN"
                     /locus_tag="OO564_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6651523.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53273..53560
                     /gene="hpf"
                     /locus_tag="OO564_000259"
     CDS             53273..53560
                     /gene="hpf"
                     /locus_tag="OO564_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6651524.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            53678..54169
                     /gene="ptsN"
                     /locus_tag="OO564_000260"
     CDS             53678..54169
                     /gene="ptsN"
                     /locus_tag="OO564_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6651525.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54215..55069
                     /gene="rapZ"
                     /locus_tag="OO564_000261"
     CDS             54215..55069
                     /gene="rapZ"
                     /locus_tag="OO564_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6651526.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55066..55338
                     /gene="npr"
                     /locus_tag="OO564_000262"
     CDS             55066..55338
                     /gene="npr"
                     /locus_tag="OO564_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6651527.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            55551..56183
                     /gene="yrbL"
                     /locus_tag="OO564_000263"
     CDS             55551..56183
                     /gene="yrbL"
                     /locus_tag="OO564_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6651528.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56180..56908)
                     /gene="mtgA"
                     /locus_tag="OO564_000264"
     CDS             complement(56180..56908)
                     /gene="mtgA"
                     /locus_tag="OO564_000264"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6651529.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(56905..57558)
                     /gene="elbB"
                     /locus_tag="OO564_000265"
     CDS             complement(56905..57558)
                     /gene="elbB"
                     /locus_tag="OO564_000265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6651530.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(57788..60124)
                     /gene="arcB"
                     /locus_tag="OO564_000266"
     CDS             complement(57788..60124)
                     /gene="arcB"
                     /locus_tag="OO564_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6651531.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60220..61149)
                     /locus_tag="OO564_000267"
     CDS             complement(60220..61149)
                     /locus_tag="OO564_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6651532.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            61824..66284
                     /gene="gltB"
                     /locus_tag="OO564_000268"
     CDS             61824..66284
                     /gene="gltB"
                     /locus_tag="OO564_000268"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6651533.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66297..67715
                     /gene="gltD"
                     /locus_tag="OO564_000269"
     CDS             66297..67715
                     /gene="gltD"
                     /locus_tag="OO564_000269"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6651534.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            67899..68378
                     /locus_tag="OO564_000270"
     CDS             67899..68378
                     /locus_tag="OO564_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6651535.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16729 a        18575 c        16988 g        16090 t
ORIGIN      
        1 ttttagcgca tcacggcggc cttttgattt ggcaggtaaa aagccttcgt aacagaaacg
       61 gtcagatggt aaacccgcgg cgcttaacgc agtgatagca gcacacggtc cgggtagcgg
      121 caccacacga atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat
      181 tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gcttcgccag
      241 cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat
      301 cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa tcagatcaac
      361 ggcctgtaat acctctaacg tacgctgggt gatatccgcc agattgccga ttggcgtcgg
      421 tacaatgtaa agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc
      481 cgtattgccg atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt
      541 accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct
      601 gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac
      661 ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag
      721 gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc
      781 ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac
      841 actgctggcg gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct
      901 ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa
      961 aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga
     1021 accgctgctt ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc
     1081 ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca
     1141 aggctggctg gctctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa
     1201 agccgggatc gccgactggc agaaacgtta tccgaacaat ccgggcgcga aaatgctgcc
     1261 aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct
     1321 gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc
     1381 cgcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga
     1441 cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt gccagcccgg cacaagcctc
     1501 ggttagcgat ctgaccggtg atcagcctgc ggcccagccg gtgcctgtaa gcgccccggc
     1561 gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc gcagagctga aaatctacga
     1621 tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat
     1681 tgtggttggt ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct
     1741 gagcgtactg gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt
     1801 cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa
     1861 acaagcgccg ctggtgctga tcccacgcag tgcattgggc gatcgcgtag ccaatgcgtt
     1921 tgcgcaagag tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac
     1981 cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat
     2041 tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac
     2101 gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtct acattgtggc
     2161 aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag
     2221 cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc cggatttccg
     2281 actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc
     2341 gttaatgcag caggcgctca gcgcggtgaa caacgattat tcgctggctc gcatgtatgc
     2401 gatgggcgtt gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg
     2461 ttttgaaatc aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag
     2521 gaagttatca tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa
     2581 cagaccggcg atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg
     2641 tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc
     2701 ctgaccaccg tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca
     2761 gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg
     2821 cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg
     2881 aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat
     2941 tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc
     3001 ggcagaggcg cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct
     3061 caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt
     3121 tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact
     3181 aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta
     3241 tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg
     3301 tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg atatgaccat
     3361 tgtggcattg accggctatg acggcggcga acttgcaggt ttgttagggc cacaggatgt
     3421 ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt
     3481 aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg attaaggaga
     3541 atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg
     3601 tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg
     3661 cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc
     3721 gaaagacgaa cagattaaga aagaaacccg tattaatgtc acggcctatc agggcaaagt
     3781 gctgctggtt gggcagtcac caaatgctga actttcggct cgcgccaaac agattgctat
     3841 gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct
     3901 gggcgaagca tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag
     3961 cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat
     4021 ggggctggta actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg
     4081 cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga
     4141 tgcgacgctt gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc
     4201 gtttacgccg catccgacat gttttccctc aaatcagaac aacagcgcca gcccgccgac
     4261 aatcacacca gacactgcca ccatcgcccc tgtcagccat aaggctttcg ccgggaacgc
     4321 tttgcgcagc ataatcagtg acggcaaact cactgccggg agcgtcatca acaatgccag
     4381 cgccggagcg gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc
     4441 cgtgggaatg acaaacaagc atcctgctac cgccatcgcc accacccaca tcaggctgtt
     4501 atcgacagca ccatcggcat gggggaataa ccagacgcgt gcggcaccca acaccagtac
     4561 tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc catagcgccc tgccccagcg
     4621 gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg
     4681 tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc
     4741 caccagacga atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc
     4801 cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg atacctgttg
     4861 gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga
     4921 aaacagcgtt cccggcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca
     4981 gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt
     5041 ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg gattagcatc
     5101 cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa acgctttgcc
     5161 gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc
     5221 gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt
     5281 ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg
     5341 gaataattat tcagctcttt tgcgtaattc tgaaaagctt aaaatcgtca cgccttcatg
     5401 ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt
     5461 ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt
     5521 gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc
     5581 aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt
     5641 ggcacccata gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc
     5701 ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg
     5761 ccgcgtggtg ccgagacaac aaaatacaat gtcgatgggt tcggtgacct gcgccaacgc
     5821 gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg
     5881 cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca gcaggtgacc
     5941 gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc ctttacagaa
     6001 ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa tagaccatga ggtaattatc
     6061 cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa
     6121 aattgccgtt ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga
     6181 gttccgtaaa gccggacacg aagtgattac tattgaaaaa caagcgggta aaacggtgaa
     6241 aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc
     6301 ggagtttgat gccctgttgc taccgggtgg tcattcaccg gattatctgc gtggcgacaa
     6361 ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg
     6421 tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcagt
     6481 taaaccgatc attattgatg ttaaaaatgc gggcgtggaa ttttacgatc aggaagtcgt
     6541 ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg
     6601 cgaggcgtta cgcctgctcg gtgcctaagt cgcgtagcca aatcattttt ttaccaaagc
     6661 ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca
     6721 gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac
     6781 cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct accgttttcg
     6841 gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg
     6901 ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat
     6961 tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca
     7021 gccaacggct gatggcgatg agtgtttcca ttgctgttct cttttatact gtgggcctga
     7081 ccttaacatg cgatgcagac gatgaccccc tggtttcttt acttgatccg taccgccgac
     7141 aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc
     7201 ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca
     7261 gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact gaccaaacgg
     7321 caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag cagcctgcaa
     7381 accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg gttacgccat
     7441 tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc ggccagcggc
     7501 aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc gccggatcgc
     7561 ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca
     7621 gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga
     7681 cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct
     7741 gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt
     7801 gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca tcaatacccg
     7861 gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt
     7921 ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg
     7981 taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacacccc
     8041 cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg
     8101 tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg
     8161 cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat
     8221 atcacacaca tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag
     8281 cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg
     8341 cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag
     8401 tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa
     8461 aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa
     8521 ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta
     8581 acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc
     8641 cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg
     8701 gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt
     8761 gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatct cgattaacac
     8821 ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct
     8881 gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta
     8941 cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa
     9001 cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca
     9061 tcaggtgaaa cagctggcac gggttactcc tgtcccgctg gaagtgtttg ctttcggcag
     9121 cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc
     9181 caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg
     9241 gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg
     9301 ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga
     9361 agaaccaacc agtctcaata ccctggaact gctgccggag ttaatggctg cgaatattgc
     9421 ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg tggcgaaagt
     9481 ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc
     9541 gtggatggag acgctcgggt cgatgtccga aggcacgcaa accacccttg gcgcgtatca
     9601 ccgtaaatgg cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg
     9661 ccaaaagaga cgctggaaga tttttatcag caagccgccg ccagcagcgc cgacgtgatt
     9721 tatcttggtg aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctgtctggag
     9781 atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg
     9841 caggcatcct ctgaactggg cgaactgaaa cgctatgttg agaacggtga gtttctgatt
     9901 gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc
     9961 gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg
    10021 atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag
    10081 tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta
    10141 ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag
    10201 tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa
    10261 caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac
    10321 gagctggcat ccatgcaggg attggttgat gtagtccgcc tgtcaccgca gggcactgac
    10381 actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgtgcc actgccgttg
    10441 acggcaaaca gcgactgtaa cggctactgg cggcggctgg caggactgga gctgcaagcc
    10501 taagtaaata gctcactttg ttaacaactt taactattct ttaatgcagt attaaagatt
    10561 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc
    10621 tggcccccat acccgaaggc tcttcagcgc gggaagcgtt ctcccactct ctcgatctcg
    10681 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    10741 ccggcattgc cagtgctgca acatcggtat tgattggcta tctggcggcg aataccacca
    10801 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    10861 aacagttcgg cacactcaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg
    10921 ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc ggcgatattg
    10981 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11041 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11101 gcctttacag tgcacaactg gcggcgcagc ttggtctgcc atttgcgttt gcctcacact
    11161 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagtaacttc aaaccgtcgg
    11221 ctcggctgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    11281 gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg
    11341 aaaccgggca actgccgccg ccgattcaaa atatggatca gttctggtca ccgtctgagc
    11401 agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg cgataaagcg aaagtgcgtc
    11461 atggtttgca gtcgatcctg cgcgaaaccg acgctgatga gattatggtt aacgggcaga
    11521 ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    11581 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    11641 taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    11701 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg caccagtgcg
    11761 ttgccgacgc caaacaccag aatcagcgca atcatcggct tgccacccca gacgcggaat
    11821 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    11881 cagatggtag ccgctaaacc agcataacca atggcataca ggaatccgtt cgggaacagc
    11941 agaccaccca caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg gcccacagcc
    12001 gagtcgtcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12061 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12121 ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat taccgccctt ctcggcgatg
    12181 ccgataaact ccggacgagg gatgttgccc attgtcgcca gcaaccagat ggtatacagc
    12241 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    12301 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccaa gcagaacggc
    12361 agggtcatca gcaaatacgg cgcataagat gcattgcttt cggcaacgtt gaacaatgtc
    12421 gcaggctgca catgccccag caggctaccg aaggtgagga agaaggtaat gactttcgcc
    12481 cccagcacaa tcgccgtcat gcggctgacg gctttagtac tcaaccacac cacaaacgct
    12541 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    12601 aaggtgtgat gcagaatcga accactggca gaaatatagg cataggtcag gatatagagc
    12661 acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    12721 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    12781 gaatgcagca tacagaacca ggtaaagatc agcgctgcca ttgaccagaa aaaccacgcc
    12841 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    12901 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    12961 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacatga
    13021 gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat attattttac
    13081 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13141 gagtcatcac gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    13201 ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgctcgcca
    13261 ccgaaaccac gaccaccgcc acgaagctca ccgccagtat gcggctgtgc atcgcccagt
    13321 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    13381 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttaccg
    13441 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca
    13501 acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    13561 tcacgacgcg gacgatcttc acggtcgcca cgcgggccac ggtcgttacg atcgcgcgga
    13621 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca
    13681 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    13741 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    13801 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    13861 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    13921 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    13981 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14041 ttaaccacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc
    14101 aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag cgcctggttc
    14161 atgtcaccgt tcagcgcggc gctattgtag ccgttacgct caagagcttc agccacttcc
    14221 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    14281 aaacgtacca gtgcttcgtt tttacgcata ccccacacag tccagtagct ctggctgatg
    14341 tcaggacggg tagtcacgct ggactgaata cgcacttcct gcggctcttt cataaagcgg
    14401 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    14461 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    14521 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    14581 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    14641 aattgcacgt cataacgctg gccgccgtac agggcaacca catttacgcc gcgcatgtgt
    14701 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    14761 accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg taaagagaat
    14821 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    14881 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    14941 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15001 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15061 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15121 tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    15181 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    15241 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    15301 tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    15361 tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc cttgagcctt tccattaacg
    15421 tgtgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    15481 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    15541 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    15601 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcaatc ccgcgattca
    15661 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    15721 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat
    15781 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    15841 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    15901 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    15961 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16021 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16081 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16141 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    16201 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    16261 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    16321 ctttctcaac gcgtttgtca gcgatttgag agatgtgtac cagaccttct ttaccgccgc
    16381 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    16441 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    16501 cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat ttcgatggtg gtgccagttt
    16561 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    16621 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    16681 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    16741 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    16801 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    16861 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    16921 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    16981 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17041 ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac
    17101 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    17161 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    17221 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    17281 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    17341 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    17401 tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    17461 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    17521 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    17581 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    17641 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    17701 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca gcggcttcag
    17761 taccggcaac aaccagatcc agtttgctct ctttcaactc gtcctgagtc gggttcagta
    17821 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    17881 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    17941 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18001 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc
    18061 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18121 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    18181 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    18241 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    18301 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    18361 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    18421 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    18481 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggcccct ttttctgaaa
    18541 ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt
    18601 ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac
    18661 gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg
    18721 cagtcagcag tgctacctga acttcagtag aaccggtgtc gtttgcgtca cgaccaaact
    18781 cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat
    18841 agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta
    18901 aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagac
    18961 ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg
    19021 tcaatttcac ccataccgat aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct
    19081 tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt
    19141 aacggaagat tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga
    19201 tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gttcgaccag ttcacgcaga
    19261 tgttccaggg tcacaatccg ttcaaccgga tatttactta ccgccagacg acgcaggtaa
    19321 ataacatgcg cgccacaacc gagtttttca cccaggtcat caatgatggt gcggatataa
    19381 gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcgtggcg aataaacagc
    19441 aattcataaa cggtaatcgg gcgcgcttca cgcggtactt caatgccctg acgcgcatat
    19501 tcgtacagtt ttttaccctg atatttcagt gctgaataca tcgaagggat ctgttcgata
    19561 tcgccacgga aagtatccag cgccgccgcc agttgctctg cgctaaaggt taccggacgt
    19621 tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg
    19681 acccgatagc gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg
    19741 caaattggca acatgccagt tgccagcggg tccagcgcac cggtatgccc ggcacggttg
    19801 gcgttataaa tccgtttcac tttttgcagc gcatcattgc tggacatacc ctgcggttta
    19861 tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct
    19921 ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca
    19981 ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca
    20041 cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcttgca
    20101 acgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata
    20161 ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca
    20221 ggcgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg
    20281 ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg
    20341 ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa
    20401 gacgcgccag cgtcacatca ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc
    20461 atggaaccct aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg
    20521 atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg
    20581 ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag
    20641 ataaccacgt tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta
    20701 accatacagc ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc
    20761 agaccgataa tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact
    20821 tcgtcaatca ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc
    20881 actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc
    20941 gcagccaggg tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact
    21001 ttaacttcgt cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt
    21061 acgtctgcct tcaggacgat gttcacttcg tgaacttcgc cttcggtcat gttggcgaac
    21121 atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga
    21181 cgatacagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc
    21241 gcagccggta cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc
    21301 acttcctgac ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga
    21361 acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga
    21421 cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc
    21481 agctccagaa cttccgcctg cagcaggata gcgtccagca gttcatcgat accagtaccc
    21541 gctttcgcag acacatgtac gaactggctt tcaccgcccc actcttccgg caggatgccg
    21601 tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc
    21661 actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc gatcgtctgc
    21721 ggcattacgc cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca
    21781 cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg
    21841 ttttcagttt caacgtggta tgcaccaatg tgctgagtaa tgccgcccgc ttcgccagag
    21901 gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc
    21961 atgatggtca caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt
    22021 accgcctctt ccagctcgtt ttcacgacgc aggataactt tatggcccat ctcttcagca
    22081 accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc
    22141 atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg
    22201 gtgatagttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct
    22261 tgctgcagcg aagaaccttt acgttttccg cctttaccgc cacgtactgc tgcgcgtgct
    22321 tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc
    22381 gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg
    22441 cgagcatgtt gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta
    22501 tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct
    22561 tcttcgagtt tacgacgcgc ttcttcttca gctttacgct tgagctctgc agcttcctgc
    22621 tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact
    22681 ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct
    22741 gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga
    22801 cgggcttgct cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg
    22861 cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta
    22921 agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc
    22981 aggtggtcaa tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg
    23041 atacctgcat cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc
    23101 agcgttttaa tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg
    23161 tcaccgaacc agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc
    23221 aacccttcga tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa
    23281 acgccacggg cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga
    23341 tcgtcagccg gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt
    23401 gcatttttag cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc
    23461 aacagctctt tcatcggcac ataggccagt tcttccagcg tcgagaaacc ttcttctacc
    23521 agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg
    23581 tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt gagttcccaa
    23641 ccgctcagct gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg
    23701 ttaccggctt caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca
    23761 acgtctgccg gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg
    23821 atatcgatac gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata
    23881 cctacgcaag cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca
    23941 cgagaacccg ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttccggc
    24001 acttcaatac ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc
    24061 gcgccacgcg cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg
    24121 aagttttcac gcggcagcat atcttcgcgc aggatcacgg cttcagcatt gttgcccaga
    24181 tccagagaga tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg
    24241 tgttcacgga actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata
    24301 acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca
    24361 acgtaatcgc ccaagttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc
    24421 ttggtcggct gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg
    24481 cttttgcgat cgatctgtac acggacgtcg atctcttgct catatttttt ctttgttgct
    24541 gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg
    24601 gatacggctt caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca
    24661 gactattaaa agtggggaac caggttcgcc ttctggatat tactcagcgc gaacacttca
    24721 tctttacctt cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc
    24781 catttacgac ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa
    24841 cgggcgtagt gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg
    24901 ttataagcaa cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca
    24961 tcagcacaat catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat
    25021 gtgcgaccgc gaataaattc gatgcctacc agttcaaaac ccagggcctc aactggcgca
    25081 gtaatcatct ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa
    25141 aagggcctaa agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc
    25201 tttatataac tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt
    25261 ttcaaatccc actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg
    25321 aaagtggttg cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct
    25381 accagactgc tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa
    25441 tgcaaatact gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag
    25501 tgatccatgc cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca
    25561 ctttcatttg ttgaatactt ttgccttccc ctgctctccc ttaagcgcat tattttacaa
    25621 aaaacacact aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt
    25681 ttgcataaaa attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt
    25741 gttattttat gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt
    25801 tttccggcgg tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc
    25861 cttatgcata tactgcaaac ctaggccagc cagacgaaga ggattatgat gcgatccctc
    25921 gtcgtgccat ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg
    25981 tggccgaagg tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga
    26041 cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga
    26101 tgaaagaaga tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg
    26161 aacgtttcta tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc
    26221 ttgatactga ctttattgat gaactgggcg gccgtcatga gatgtctgaa tttatgattg
    26281 cctgcggttt cgactacaaa atgtctgtcg aaaaagccta ctccacagac tccaacatgc
    26341 ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca
    26401 acccgattat gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca
    26461 cagtacgctt tgagcaaggt catccggttg cgctgaacgg taaaaccttt agcgacgacg
    26521 tagaaatgat gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc
    26581 agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg
    26641 cactgctgca cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa gacaccattg
    26701 agcagtatca cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg cgttggtttg
    26761 actcccaggc gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg
    26821 gtgaagttac cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag
    26881 agaacctgac ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc
    26941 cagatgatcg tattggtcaa ttgaccatgc gtaatctgga tatcactgat acccgagaga
    27001 aactttttgg ttatgccaaa actggcctgc tttcctcctc tgccacttca ggcctgccgc
    27061 aggtggagaa tatggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt
    27121 ggggttacct tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc
    27181 atggtttgca tccacaaccc gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt
    27241 ttgcggctgt tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc
    27301 tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca tccccaagaa cggggctata
    27361 acagataaac atcgggacat gatatgcctg ctggctagcc tccctgcctc catgaaaata
    27421 gacgttcttc ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc
    27481 gcggcgatct tttaataatt caaaaaactg acctagcaaa ctatctgtgt aatgaatgga
    27541 gttgtcatag caggcatcct gatcgtcctg cggttgaaac acgcgctgga ttgcggatag
    27601 gcgctacaag ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc
    27661 gtattttgct gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca
    27721 taaactgttt ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa
    27781 gcggattgcg agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg
    27841 ttgtcggggt aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga
    27901 accgacagcg cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt
    27961 ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg
    28021 acacgtacag actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat
    28081 aattgaaaat acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct
    28141 aaagcaaaat agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata
    28201 tatggactga acgctttttt attttttgca tctttataag caaattgaca agcggaaaat
    28261 aaactgcccg aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa
    28321 acatcatatt ttattattac tgccaaaaaa aaaagggata aaaaagcaaa agcacataga
    28381 taagggatat acatccccag cattttcaca acttcatccg gatcactctg cagcacacta
    28441 atcgcaaatc catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc
    28501 atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat
    28561 gatatacgtt taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc
    28621 gctccggaag aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac
    28681 caatgagatt taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt
    28741 cacaaccaat ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac
    28801 tctgaaccat atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg
    28861 cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc
    28921 atcagtagtt atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc
    28981 gtaccggaac tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt
    29041 ataactaagg cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga
    29101 cgagatacag atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt
    29161 ttcagcgtct cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat
    29221 tgggcactac cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta
    29281 ctttttagtt cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg
    29341 ttttcgccgg tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga
    29401 tgttacccag caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg
    29461 tcatgaagtt accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg
    29521 aggctcccat atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg
    29581 ccacaataag gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt
    29641 tgccgcgtac caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg
    29701 ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc aattttatcg cattgtatca
    29761 actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc
    29821 ctttttagcc agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc
    29881 aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt
    29941 gccggattta cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt
    30001 cacggcttta actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt
    30061 aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt
    30121 acgtgccatc gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt
    30181 atccagcagg atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat
    30241 tttttccagt acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt
    30301 cagcgccagt tcaagcccca tgttgctcat caatgtaccc acagcgccac cacgcagctg
    30361 gccctgacga agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt
    30421 gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc
    30481 cgctttttca gccagcacac gagcctggag cacgcgaacg tcggtagccc ccacttcggc
    30541 attgatgttt acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg
    30601 cagcacgttc ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag
    30661 ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc
    30721 ggcatcaacg atacggctgg ctttacccag ttctgccgaa tcaacgcagc tgatctcctt
    30781 ttccatttcc gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga
    30841 gaagaattta atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc
    30901 ctctgcgcgg aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa
    30961 gagtgcggaa aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc
    31021 agaaatacgc gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt
    31081 acccgcggcc caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg
    31141 accacgaatc ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg
    31201 cagacagagt ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac
    31261 gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc
    31321 gctcggacgg ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca
    31381 acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg
    31441 agagattttt accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg
    31501 cctgctcgca acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt
    31561 catacttcgg agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg
    31621 gtaaaccggt ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat
    31681 taataatgtg agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga
    31741 ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca
    31801 actcttcttc aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa
    31861 tgatcgtcgc gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt
    31921 tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag
    31981 gatggctaag gtccagtgaa gtaccctggg caaagagttt catgatgtta tccctggtat
    32041 gaattgataa gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc
    32101 tgatgcggga acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt
    32161 acgcggttca tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga
    32221 agcgcctggt tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc
    32281 aatctgcggt gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat
    32341 atccatattg tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt
    32401 cacttcctgg tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct
    32461 acggccgagg aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga
    32521 gaagccccac tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga
    32581 cgcaccggta gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta
    32641 cagcgtagaa atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg
    32701 caagaagaaa gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg
    32761 atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga
    32821 ttctttctgc gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc
    32881 tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag
    32941 tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc
    33001 aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat
    33061 ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg
    33121 acgcaacagg gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc
    33181 ttcgttacct tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc
    33241 atcgtgacca ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat
    33301 aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc
    33361 acccacaccg acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt
    33421 cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat
    33481 caggacgcct ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata
    33541 ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt
    33601 cgttttgatc tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc
    33661 tttgccaccg ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag
    33721 catcgggaac caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc
    33781 gacaaccttg acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat
    33841 gtaagtggta taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg
    33901 cgcttcacga acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg
    33961 gccattagac tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac
    34021 cagccagagt attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg
    34081 tgttaaaaac agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc
    34141 tcccggttag ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga
    34201 agagtccggc ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc
    34261 atcgaaacct tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc
    34321 acgacacatt tctagcgcca gttccaccag atacatggca cgggggatat ccaccgccgg
    34381 tgttccgctc atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac
    34441 gcgctccagc agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac
    34501 accaacgata ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat
    34561 ttgggtgacc acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat
    34621 tcccggttta aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga
    34681 acgtaatccc tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca
    34741 gcggctggag ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc
    34801 atcgtaacca attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt
    34861 agaatgaccc gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac
    34921 aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta
    34981 atggtttgac cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca
    35041 tcaaggtgaa aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg
    35101 tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc
    35161 caactaaaaa acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt
    35221 gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt
    35281 gataaaaggc cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat
    35341 tgggtaattc ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg
    35401 ttttgatgac cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt
    35461 ttacagaaat caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct
    35521 gttcttcgtc agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat
    35581 tgttgggcat tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt
    35641 tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt
    35701 tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag
    35761 atttcagaaa atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag
    35821 cttgcatttg ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct
    35881 gttcggcctg cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg
    35941 tacaagtccc aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg
    36001 aagggtcatc gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt
    36061 gttagcgcga gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt
    36121 tcagtgttca ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc
    36181 ttgccctgat ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc
    36241 agatggcgct gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac
    36301 tcggccccga ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg
    36361 tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata
    36421 ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt
    36481 tgatagatac ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca
    36541 tgacacaatg ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg
    36601 tctcgctcta cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt
    36661 accccgttac gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat
    36721 actgcgaact ggatgtggtg ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc
    36781 cacaacgttc tgagccgctc ccgttggctt ttggcgtgca ggatggagcc agctatgccg
    36841 gtgcaattct gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc
    36901 gccagactca ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc
    36961 acgatctgct taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc
    37021 gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg
    37081 tgcgtcagat cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg
    37141 gttcagggct ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat
    37201 acattgccca acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg
    37261 acggctcttt gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag
    37321 cgaaaaccgg ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg
    37381 ggcaacgaat ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc
    37441 gtaatcgccg tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga
    37501 acaattagtc aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc
    37561 gctttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt
    37621 caatatattg caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat
    37681 tggtcattag tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc
    37741 agtcgtcatc ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat
    37801 aatcatccca catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg
    37861 ggttttcaat gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg
    37921 ccgcagagat cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt
    37981 tctcttcggc ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt
    38041 tcgccgccag atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa
    38101 ccggatcggt gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt
    38161 ccaggtgctt caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc
    38221 ctggaatatc ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg
    38281 gcaccagagt ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa
    38341 taaaggtcga tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca
    38401 gctccagcag cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac
    38461 ggttaactga cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca
    38521 gcagacgctg accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta
    38581 cacgcgtacc taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc
    38641 ggcttgcgcc gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa
    38701 gcgtgttcag gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc
    38761 catccgggcc gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc
    38821 catcacctgc aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa
    38881 atcattcgcc tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa
    38941 tacgatgacc aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac
    39001 ctaaaaggtt taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg
    39061 aaaaaaacag cgtaaacagt ggggtgagcg tgatgatcgc gctcacctga gctgcctgcc
    39121 agcgagccat cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa
    39181 ttaaacatgc gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga
    39241 agagcgcgat tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac
    39301 gcaataaaac cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa
    39361 agataactcc ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa
    39421 aaaacatcac cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt
    39481 tctctttcag gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca
    39541 cctgcgaagc ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa
    39601 acagcccggc ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta
    39661 atggcggcaa cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga
    39721 aacggtaaaa cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg
    39781 gcaacgcccc ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct
    39841 gcttcatacc gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt
    39901 ttaccggtta tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg
    39961 ggacgcgaaa aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa
    40021 cttcgaattt cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta
    40081 cgttagcgcc agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa
    40141 cggattcgcc accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac
    40201 gtgtggagcc gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct
    40261 gatgccagta attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg
    40321 tttacgacga cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc
    40381 agctttgatt acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc
    40441 gatcatcagc acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag
    40501 gcgaacggtc tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta
    40561 catataaaac tccgcttccg cgcacacctt ttcaatgatt caaagtgcgc tataaatatt
    40621 cacaataggg cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac
    40681 acgaagaaaa aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa
    40741 ttaccctata ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg
    40801 gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat
    40861 atggcgggtg ttaatgcggc aatccttgag cagcttaatt ccgacgtcca actgatcaat
    40921 cagttaggct attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta
    40981 ctggctgcac gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc
    41041 gagtttatcc acacggcgac tctgctacac gacgacgttg tggatgaatc agatatgcgc
    41101 aggggtaaag ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat
    41161 tttatttata cccgcgcgtt ccagatgatg accagcctcg gttcactcaa agtgctggaa
    41221 gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt
    41281 aacgatccgg acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt
    41341 ctgtttgagg ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaggaggag
    41401 aaaggcctgc aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta
    41461 ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa
    41521 ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag
    41581 atgatccgta ccgccatcga acaaggtaac ggtcgccatc ttctggaacc ggttctggaa
    41641 gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga agaagcagac
    41701 aaagccatcg cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc
    41761 ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg
    41821 cgggatgatt ccagtaaatt ccataaaaac actaattcag cgctataaca cctgaaaaaa
    41881 agggcgatga atctttatat attctgaata ttcacactct ttacaggaac tttttagcgc
    41941 aataggccat caggagtata gtgatgcccg acagaagaag tgttctgaat gaaagcgact
    42001 aacttaagga gtgaggaaag atggaaagta atttcattga ctggcatccc gctgacatca
    42061 ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga atctcgcaga aatggtttga
    42121 gttcctcaac gctggcaaac gcattatcgc gcccatggcc gaaaggagag atgattattg
    42181 cgaaagccct gggaactgac ccctgggtta tctggccatc acgctaccat gatccgcaga
    42241 cccatgagtt tatcgacaga acgcagttga tgcgtagcta cactaaaccg aaaaaatgag
    42301 tggtctggcg gtagccccgc gaacggggct gccagctctc agacgattac tcgcctttca
    42361 cacgctcaat atttgcacct aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat
    42421 cgatgtgata aatacgatca accaccgtcg tcccttccgc aatacagcca gccagcacca
    42481 ggcttgctga tgcacgcaga tcggttgcca taacctgtgc accagaaagt ttttcaacac
    42541 catgacaaat aacggtattg ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg
    42601 gcacatgcat aaagcggttt tcaaagaccg tttcggtgat gaacccggtc ccttcagcca
    42661 ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg
    42721 tacgtacgtt aacagccttc ggacgtttgc catgcatatc caggctaatc cagtcttcgc
    42781 cgacttcgat gtccgctcca gcatcacgca gtttcgccag cacggcgtcc agagtatctg
    42841 gctgcgcgtt acggcagata attttgccac gagagatcgc cgccgccacc aggaaagtac
    42901 cggtttcgat acgatccggc agcacgcgat agacaccgcc gcctaaacgt tccacacctt
    42961 cgatgacgat acgatcggtg ccctgaccgc taattttcgc acccagcgta atcaggaagt
    43021 tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg
    43081 ccagggttgc agcacacatg atggtcaccg ttgcgccaac gctgacttta tccatcacga
    43141 tatgtgcgcc tttcagacga ccatcgacgg aagctttaac gtaaccttct tccagtttga
    43201 tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc aaccggacga gcaccgatcg
    43261 tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg cgctaccagc ggccccagcg
    43321 cccagataga agcacgcatg gttttaacca gatcgtaagg tgcgcagaat acattaacgt
    43381 cgcgggcatc aatatgcaca gaaccattac gttctacttt cgcacccagc tggcttagca
    43441 gtttcattga tgtatcgacg tctttcagtt tcgggacgtt ctggatctct accggttctt
    43501 ccgccagtag tgcggcaaaa agaataggca gagcagcatt tttagcgccg gaaattgtga
    43561 cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt atccatttag tttgttctca
    43621 gttaacaatt catatccgct accggcgaat cgcccatagc tcaaaagccg ttcagtttgc
    43681 gatcgcgcgc ccactccgca ggggtatacg ctttgatcga cacagcatga atgcggttat
    43741 ccgcaatata ttccatcagc gggccataga ccgtctgctg ttttttaacc cgactcatac
    43801 cgtcaaacaa ctcacccacg gcaataacct gaaagtgact gccatcgccg gaaacgtgga
    43861 cttcctggag ggagagagcg ttcatcaaca cgctctgaat ttcattattt tccatgggat
    43921 cttcaatcat cagttaataa accagcgaaa catcttagag caaagttgcg ctggcataaa
    43981 taagcaaaaa gcctcgctga taaatcagac aaggctcgac ttgcaggcag gtttgccgga
    44041 caggcggtta acgccatatc cggcctgaaa aaatttaacg aggcagaaca tcagcaggca
    44101 aattatacaa tttcgcgagg gtatacactt tgtcgtttac cccctgaagc gtcacgttgt
    44161 tgccctgctt tttcgccaga tcgataagat ggagcagcag tgccagtccc cccgtatcca
    44221 cgcgggacac acggctaaga tcgatgcagg taatcccctt caccgcttcc tcacgcattt
    44281 cccaaagcgg tagcagaacg tcctgatcca gctctccgga taacgccagc gtgtcacccg
    44341 tctgcatcca gctcagtgac tcgctcatta ttttttctct tccagagtga ttttctgttg
    44401 agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc
    44461 ccactcgttt tgtttggtgg tgatcatact gacgccttca gcaatcatgt cgtaagcttg
    44521 ccagtttccc gtctgggagt ttttacgcca ctggaagtcc agacgcaccg gcggacggcc
    44581 attcgggtca ataatggtaa cgcgaatagg cacaatggtt ttatcgccca gcggctgttc
    44641 tggcgcaatc tgataggttt gaccgtgata catcgccagc gcctgaccgt aagcctgctt
    44701 caggtactca cggaaagcgg caaagtaggc ttcacgttga gcaggggtcg cactcttgta
    44761 atactggccc agcaccagcg caccggcgta tttcacctgt acgtatggca gcagttcctg
    44821 atcaacaatg gtacgcagat aatccgggtt ggcccgaatt tgcggttgct cattcttcag
    44881 gcgatcgaac gttttctgcg ccgcctcgtc catcagctta taaggattgg tctggtctgc
    44941 cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg accatcatta aacgtttaaa
    45001 catgcgtcgg ttctcctgaa attatttcgt tgtacccaca ggttcagtgg tttcattatt
    45061 acctggcgca gcagctggcg catcgccact attcttattg tcatcgcctt tactaccgta
    45121 aaggaactga ccaatgagat cttccagcac catcgcagac ttggtgtcct gaattgtatc
    45181 gccatccttc aggatagcag tccccagttc cgggtcttca aaaccgacgt ttaatgccag
    45241 atattgttct cccagcaggc cggaagtacg aatgctcagc gaactggtat caggaatgtg
    45301 gttataacgt tgttcaattt ccagcgttac gcgcggcaga taggttttcg ggtccagcgt
    45361 aatatccgcc acccggccca caacaacacc accaatgctg accggagagc gcgctttcag
    45421 gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc gattcagtac gtatggacgt
    45481 cacgttcgcc gccttcaggc aaacaaacag cgccgccagc agtgctgcta ataaaaagat
    45541 acccacccaa atttcatttt ttttcgtttg catgaactca attcccaaac atcaatgcgg
    45601 tcagcacaaa atccagcccc agaacagcca gagacgagtg gacaacggtg cgagtggttg
    45661 cccggctaat cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg
    45721 tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt gaccagatcc atacgccagt
    45781 cgacggcatt ttgcattgcc gaccagaaga acccgctatc aatgcctttc caactgacgc
    45841 cgaccagcga tccgccccag atccccacgg cgacgaagat aaccgtcaac agtggtaatg
    45901 aaataacccc agcccagaaa cggggagaaa taacccgacg cagcggatcc accgccatca
    45961 tctccatact ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg
    46021 aaccagcacg cccggcaaac aacagcgcgg caaccaccgg ccccagttca cgcagtagcg
    46081 ataacgccac cagcataccc agactggttt ccgcgctata agtggtcaga accagataac
    46141 cttgcagccc caacaccatt ccgatgaaca cgccagaaac tacaataatc agcatcgaca
    46201 ggacgccgac attatagagc tggcgcacca gcagcggcgc atgtttgcga aattccggtt
    46261 tgccgaccag cgcattgaat aacattaacc cggcccgccc gaacgttctc agggttttaa
    46321 tccctttatg tccgagcgac gccagcgcat ttaacagcat gagtggctta actccctggt
    46381 aaaagatcag cgtgataatc gccggcagga tagcggaacg gaacaggccc gtcagctatc
    46441 ccgtccagaa actgacgtac gcgcggatca ggattcgtct gcaacgcctg ggcactgcca
    46501 tgagcgacaa tttttttgtc cgccaggatc caggcgtgat ccgcaatact taacacttcc
    46561 ggcacatcgt gagaaaccac cacacaagtc acgcccagcg cgctgttcag ctcagaaatc
    46621 agcttcacca gtacgcccat ggtgatggga tcttgcccaa caaaaggttc atcaaacatg
    46681 atgagatccg gctccagcgc aatcgcacgc gccagcgctg cacgccgcgc catcccaccg
    46741 gaaagttcag aaggcattag tttagccgct ccacgcagcc ccacggcctc cagcttcatc
    46801 atcaccgtac tatgcaacaa tggcgcggga agttgggtat gttcgcgcag tggataggcg
    46861 acgttgtcaa atacgttcat atcagtgaac aacgccccgg actgaaataa catgctcatc
    46921 cgtttacgca ctgtatacag gcgcgaacga gacatcgccg gaatattctc accatcgaaa
    46981 aggatctcac catgatctgg tgcgatttgc ccgccaatca gacggagtag cgtcgtttta
    47041 ccgatgcccg atggccccat gatcgccgtg atcttccctc gcggcacggt cagggaaata
    47101 ttatcgaaga tgcagcgatt gccacgcgta aaactgacat cgcgcatatc gactaaattc
    47161 gccacagact gctccataat tcacccttcg tcttgcgttg attttctaag catggcgctc
    47221 aatttaacct tgaacccaac atatttacag aatattaccc gccgtggtta gcgaaagctg
    47281 gcatttgttt tactttttag ccgcataaag tcaaaattaa gaatccgtta cggctttctg
    47341 aaaatcttca gcggaccggc gagtatacct gaagaaagga cgttagatgc ttttagctac
    47401 ggcactgtta attgttggtt tacttttggt cgtttacagt gccgaccgcc tggtttttgc
    47461 cgcgtctatt ctttgccgaa cctttggcat cccgccgctg atcatcggca tgacggtggt
    47521 cagtattggt acatcgttac cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca
    47581 acgcgattta gccgttggta cagccctcgg ctcaaacatt atcaatatat tgctgatcct
    47641 cggtctggct gcgctggttc gtccttttac cgtccattcc gatgttctgc gccgtgaatt
    47701 acccttaatg ttgttggtca gcgtggtggc cggttccgta ctctatgacg gacaacttag
    47761 tcgaagcgat ggtatctttc tcctctttct ggctgtgcta tggctgctgt tcattgttaa
    47821 acttgcacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    47881 cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc
    47941 cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    48001 gacgatgggt ctgacggcaa ttgctatcgg aaccagtctg ccggaactgg caaccgcaat
    48061 agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat
    48121 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag agattgatcc
    48181 actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    48241 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    48301 tatcatatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    48361 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    48421 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    48481 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    48541 aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat
    48601 ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg
    48661 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    48721 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    48781 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    48841 caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    48901 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    48961 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    49021 ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta
    49081 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    49141 gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    49201 cggggggaat acgtgtgcgc cctggcattc tggccgttga ggcactgaac ttaatgcagt
    49261 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    49321 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    49381 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc
    49441 agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta
    49501 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    49561 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    49621 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    49681 gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt
    49741 tggcgatgat ctcatcgact gcccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    49801 cgatgcccat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg
    49861 tggcgcagtg cgcgaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    49921 caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct atcactggcg
    49981 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    50041 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    50101 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    50161 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    50221 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    50281 gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa tcacgacgga taacgcgcag
    50341 atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    50401 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    50461 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    50521 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    50581 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    50641 taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    50701 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggatacggta aaccggcaac
    50761 gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc agatgcacta
    50821 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    50881 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    50941 cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa
    51001 caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    51061 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    51121 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    51181 ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    51241 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga
    51301 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    51361 tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca
    51421 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    51481 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc
    51541 cggggttgac ccgatctcgg ttatcgatat taaacgcatc attgaacacc tgcgcgacag
    51601 cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    51661 cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag aaatcttaca
    51721 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    51781 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    51841 cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct gttgcagttg
    51901 tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag
    51961 caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga aacgctggac
    52021 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    52081 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgacgag
    52141 ctgccggtct accagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc
    52201 gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt
    52261 gatgaaaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    52321 gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgatccggtc
    52381 ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataag
    52441 accacgccgt ggctggaaga ggccagattg atcattagcg atcatctcga tctgttagcc
    52501 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    52561 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    52621 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa
    52681 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    52741 aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg
    52801 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    52861 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    52921 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    52981 tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat
    53041 accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cactggtgaa gaaattaatc
    53101 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    53161 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    53221 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    53281 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttacagctaa
    53341 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    53401 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    53461 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    53521 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    53581 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg
    53641 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    53701 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact gtcagagcaa
    53761 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc taccgcctca
    53821 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    53881 tattgccatt ccgcatggca aacttgaaga agatactctg cgtgccgttg gcgttttcgt
    53941 tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    54001 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    54061 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    54121 gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa
    54181 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    54241 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    54301 ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg atcgcgagat
    54361 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    54421 ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg atgccgaccg
    54481 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    54541 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    54601 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg
    54661 tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt
    54721 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    54781 gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    54841 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    54901 gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg gttgtaccgg
    54961 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    55021 taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    55081 actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    55141 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa
    55201 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    55261 gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct ctttaattct
    55321 ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa gctcccccga
    55381 caataagaaa atcacgtact gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg
    55441 cttttcactt tcagaagaac cttaagaaaa ccttaagagg cattgtttag gttttgttta
    55501 agttaatcga ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct
    55561 tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag
    55621 atgcccaacg ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc
    55681 gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac
    55741 cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg
    55801 ccgattttga cggcaagcct tccattacgc tgaccgaatt tgctgaacaa tgccgttacg
    55861 aagaagacat tgcacaactg cgccagctac tgaaacagct gaagcgttat ttgcaggata
    55921 accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt
    55981 cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga
    56041 cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc
    56101 aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg
    56161 ccctcacttc gcgcgaagct taatccagct ggtgttgttg cataaacggc tcaccgccta
    56221 actgatacat ctgccgtaaa atccacgcct gacggctacg cacgtagccg tcaggcgtgg
    56281 agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg
    56341 taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac
    56401 cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg
    56461 tttctatccc cagcgttaat ccagcttcca gcccttttcg tacccagcta cgcccatccc
    56521 ataaaaagag atttttggcc gtctgctgag aaatcgttga agcaccgcga atacggtttt
    56581 cattgcgctc gttgtgcgcc agggctttct caatggaagc gacatcaaag ccccagtgct
    56641 caggaaattt ctgatcttct gcggcaatca ccgccagtcc catccacggc gagatttgat
    56701 ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc caggcgctga
    56761 cctgtcgctc gaccattacc gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc
    56821 ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac
    56881 taaacaccgt taagcggctt ttactcattc agccagaacc agcacgcggg aaaccagctt
    56941 atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac
    57001 aatcttattg tcttcatcaa ccacgatatc atcgacagga cacggcacat gctccgcgcc
    57061 catctcttcc agtacttctg cggtatcgat atcagtaccg atggtcaaac gcagcgggaa
    57121 atcgaaaatt ttcggcagca tcgccggggc aatacacata aaaccaagcg gttttccggc
    57181 ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt cgctaccaag
    57241 actggcaaaa ttgcttaaat tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc
    57301 cagttcagcg gcatcggcct gggccagagg acggatttca ccgcgcgtta ttcgtgccgc
    57361 ttcaatcagc acattgcgcg tttccgtcat cgcttcgcca gttaaatggt tgataacatc
    57421 aacctgctgc ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa
    57481 caacgtcaac accgcttcat gaatttcaga accgtcatag acgccgcatc cgctcagaat
    57541 tacgccaatt ttcttcattg tgatcatcct ttcgcaattg actgaaacac attaattttt
    57601 ttaataaaaa tggtacgcat cacacattta actgattcat gtaacaaatc atttaagttt
    57661 tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat
    57721 aatttacggc gcagccaaga tttccctggt gttggcgcag tattcgcgca ccccggtcta
    57781 gccggggtca ttttttagtg gcttttgcca cccacgcttt cagcacttct acgtcgtgac
    57841 gccactcttc tttcatctct tcaatccatt caccgacgtt atcttcccag gcgggaaggt
    57901 caggagactg aatttgctga cccagctgtt gcagatggcg taaccccact gaccccgccg
    57961 cacctttaat tttatgtcct tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg
    58021 actccagcac gctgacatag cccggcatca tcttctcaaa caccgctaac ccgtcggtga
    58081 tcagcttcgg tcctacaagt tcgagatact gttccagcat gggaatatcg agcaatgctt
    58141 ctgatttact gttctcttct gtcgtcaccg tactcttctc atcatcctgg gtatcccaga
    58201 atttcttgat catcgcggtt agcgccggaa ccgaaagcgg cttactcagc acatcatcca
    58261 ttccagcatt gaggtactct tgtttgtctt tcagcacgtt agcggttaag gccaccagcg
    58321 gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg agagatatcc agcccggtca
    58381 tatctggcaa ctgaatatcc agcaacacca ggtcgtattc gcccggttta aacatctcca
    58441 gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc taatttttcc agcacagaac
    58501 gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag cacattcagc gccggtaaag
    58561 gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc caccgacggt gcgtggatcg
    58621 tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg ccgcccatat
    58681 ttttcgccag acgacgagaa acggccagac caataccggt gccggtggca ggtttaccgc
    58741 catgactgtc tttcacctgg taatacatgg cgaagatttt atccagctca tcctgcggaa
    58801 tgccgatacc agaatcttcc acttcaaaat gcagcatatc gccttcatcg tagcgcacgc
    58861 gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt actgatgagg ttccacagga
    58921 tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg taatggcagc gtaggctcca
    58981 ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga gagattttcc agatcggcaa
    59041 ggaagctggt gaaatcaacc ggctgattat caagctggac cttgcgccgt tccatcttat
    59101 ccatgtcgat aatatcgtta aagatattcc ccagcgtgac ggccgaaaca tggatagttt
    59161 tgagatattt ttcctgctcg gcggtgagtt cggtatccag cagaatgcgg ctcaggccga
    59221 cgataccgtt cagcggtgta cgcaattcgt gactgatggt ggaaataaac gtcgttttgt
    59281 cgcggctggc gcgttcaagc gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa
    59341 agcccatcaa accgtgacgt ttacccacgc ggtcgtagta cggcacttta cggatttcaa
    59401 agcaggcttt gcgcccgtcc gggtaatcca gccactgttc ataggtcagt gacacattat
    59461 gacggaacac tttttcatcg gtttcaatga cttttgcggc ggcttccggt gagtaaacat
    59521 cagcaggttt cagatgaacc agttgttttt cgctttttcc ggtcagcagc tccatcgcgc
    59581 ggttacagcc ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcat
    59641 caaggaagga acgtaagaat gaggattgct gctcgagctg aatttgtgtc tcttcgcgct
    59701 ctttgatttc aattttcagt tggccgaagg tttcctgcag ttctgcttcc gctttttcac
    59761 gaacggcaat ttcctgattt agctgggcaa tattatcttt taactgaacg ttgaggctca
    59821 aatcgcgctc gcgcatctcc tccagttttt gcaccagccg tgacagacgt tgtcgtgact
    59881 cctccagttg ctcgacgacc accgatagaa agtagaccgc ccacggcgta atcagcaaac
    59941 caaagaagat agaacgaata acatcaatgc tttcgacctg accatgcagc accatggtta
    60001 ccgccatttg taccacaatg gcaagaacga cgagggccag cgccagcaac attgagaagc
    60061 gcaccagacc taacttcatc atcaggtcaa cataatactg cgccagcaga cgaatttgct
    60121 tcattaggga attccttcac gacaacctgc cccaataata cccaattctg acagttacgt
    60181 tataaattgt gtgagaaatg cggaattccc ctcaacgacc tactccgttg gaggtagcca
    60241 cggacgtccc agcgccgatc cctgtacgcc atgttcattc agatagcgat ccagctcgac
    60301 cattcccgtc catcgatttt cgcaccacag cggagcaagc agcgtcgggc gacgggcgct
    60361 ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg cgaatcattt ctccggcagt
    60421 gagcgtataa tcctccagtt caataccgtt caaacgcccc gcttcccagg ctttcgccat
    60481 aatgctgcct ttcacaatat gcagcggatg cagctttatg ccgtccacgc tggtttcaac
    60541 cactcgctcc agcgtttgca aacattcagc ctgcccttcg ccaggcaggc cgacaattaa
    60601 gtgggaacac accttcagcc cgcgctgacg tgccagctgg gttgtacgct gataacaggc
    60661 aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg tgggcggttt gtagccccag
    60721 ctccagccac acttcgtaac cctggtcctt atattcgcaa agcagatcca gcaccgcatc
    60781 cggcacgcag tccgggcggg taccaacaca caaaccgaca atattggcct ggctcaccgc
    60841 ctgctgatac atagaacgca gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa
    60901 gtaggccaga tagcgtttag cgcggttaac taaattcgcc tggtgcgcca gttgctcggc
    60961 aatggaacga tgctgctgcg cttcatcggc aaacgaggca acattacaga atgtgcagcc
    61021 accacgcccg atggtaccgt cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt
    61081 atgcaccttt tgcccataac gacgggtgag atcaccacca aacatattga ctaatttctg
    61141 taactgcata atctgataga ccgcgccttg aaaagaggcc aaagcctgcc atttttagct
    61201 caattcggcg atgacctaga tcaatcgtcc cgccctgctt ttatcaactg cataatcaat
    61261 caaaattacc gaaatttcat gcataatcac ataaatcact tttgcttatc ttgtgtcaga
    61321 tttttttatc tccttatgga ttttaggcaa aaacagtagc atgaaacgtc attaccaatt
    61381 aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc aggataaggg ttaacacgcc
    61441 tttatgacag tcaggaattg actgtttctc taacgactct ccttttagcc ttaaagataa
    61501 aatctatttt aatttcagcc atttaataaa gaattttgcg ctaaagcaca tttctgtacc
    61561 aataagcttg ccatttgacc tgtatcagct ttcccgataa gttggaaatc cgctggaagc
    61621 tttctggatg agcagcctgc tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg
    61681 tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca
    61741 aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc
    61801 agagcctggg ggaggttcac gatatgttgt acgataaatc ccttgagagg gataactgtg
    61861 gtttcggcct gatcgcccac atagaaggcg aacctagcca caaggtagtg cgtactgcaa
    61921 tacacgcact ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg
    61981 acggttgcgg cttgctgtta caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc
    62041 gcggctggcg tttagcaaaa aactacgctg tcgggatgct cttcctgaat aaagatcctg
    62101 aactcgccgc tgccgcacgc cgcatcgttg aagaagaact gcaacgcgaa accttgtcga
    62161 ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct gggtgaaatc gccctctcct
    62221 ctctgccacg cattgagcaa atttttgtga acgccccggc aggctggcgt ccacgcgata
    62281 tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag
    62341 acttctacgt ctgtagcctg tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg
    62401 cggatctgcc gcgcttttat ctggatcttg cggacctgcg tctggaatcg gccatttgcc
    62461 tgttccacca gcgcttctcc actaacaccg taccgcgctg gccgctggcg caaccgttcc
    62521 gctatctggc gcataacggt gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg
    62581 cgcgtaccta taaattccag acgccgctga tccctgacct gcacgacgcc gcgccattcg
    62641 tcaacgaaac cggttctgac tccagttcca tggataacat gctggaactg ctgctggcag
    62701 gcgggatgga tatcatccgc gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc
    62761 cggatatgga cccggaactg cgtgccttct ttgactttaa ctccatgcat atggagccgt
    62821 gggatggtcc ggcgggcatc gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc
    62881 gtaacggtct gcgtccggca cgctacgtca ttaccaaaga caagctcatc acctgcgcct
    62941 ctgaagtcgg tatctgggat taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc
    63001 caggcgaact gatggtgatc gacactcgca gtgggcgtat tctgcactcg gcagaaaccg
    63061 atgacgatct gaaaagccgc cacccttata aagagtggat ggagaaaaac gtccgccgac
    63121 tggtaccgtt tgaagatctg cccgatgaag aagtgggtag ccgcgaactg gacgacgaca
    63181 cgcttgccag ctaccagaaa cagtttaact acagcgcgga agagctggac tccgtaattc
    63241 gcgtactggg cgaaaacggt caggaagcgg tcggttcgat gggcgatgat accccattcg
    63301 ccgtgctctc cagtcagccg cgcattattt acgactactt ccgccagcag tttgcccagg
    63361 tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta
    63421 tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta
    63481 aatcgccgat tctgctctac tccgatttca aacagctcac gacgatgaaa gaggagcact
    63541 accgcgcaga tacgctggat atcacctttg acgtcactaa aaccacgctc gaagcgacag
    63601 tcaaagagct atgcgacaaa gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc
    63661 tctccgaccg gaatatcgct aaagatcgcc tgccggttcc ggctccgatg gcggttggcg
    63721 cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa
    63781 ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct gggtttcggc gcgacggcta
    63841 tttatccgta ccttgcctat gaaacgctgg gccgcctggt agacacccac gcgattgcca
    63901 aagattatcg taccgtgatg ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa
    63961 tcatgtccaa aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag
    64021 cggtcggtct acacgatgat gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg
    64081 gtggggcaag ctttgaagac ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc
    64141 tggcgcgcaa gccgattagc cagggcggcc tgctgaaata cgtccacggc ggcgaatacc
    64201 acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca
    64261 gcgactatca ggaatacgcg aagctggtta atgagcgtcc gacaaccacg ctgcgcgatc
    64321 tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg
    64381 aactgtttaa acgctttgat accgccgcga tgtctatcgg cgcgttaagc ccggaagccc
    64441 acgaggcgct ggcggaagcg atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg
    64501 gcgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg catcaagcag gtagcttctg
    64561 gtcgcttcgg cgttactccg gcgtatctgg tcaatgccga cgtcattcag attaaagtcg
    64621 cccagggcgc gaagccgggc gaaggcggtc agttgccggg cgacaaagtc actccttaca
    64681 tcgccaaact gcgctattcg gtgcccggag tgacgctgat ctccccgccg ccgcaccacg
    64741 atatctactc tatcgaagac ttagcgcagc ttattttcga cctcaagcag gttaacccga
    64801 aagcgatgat ctccgttaag ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg
    64861 tggcgaaagc ttatgcggac ttgatcacca tcgcgggcta tgacggcggc accggcgcaa
    64921 gcccgctgtc gtcagtgaaa tatgcaggct gcccgtggga actcggtctg gtggaaaccc
    64981 aacaggcgct ggttgccaac ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc
    65041 tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg
    65101 gcactggccc aatggtggcg ctcggctgta aatatctgcg tatttgccac ctgaacaact
    65161 gcgccacggg tgtagcaact caggatgaca aactgcgtaa gaatcactat cacggcctgc
    65221 cgttcaaggt gactaattac tttgagttta tcgcccgtga aacccgcgag ctgatggcac
    65281 agctgggagt aacgcgtctg gtggatctga ttggtcgcac cgacctgctg aaagagctgg
    65341 acggtttcac cgccaaacag cagaaactgg cgctgtcgaa gctgctggag actgccgagc
    65401 cacatgcagg taagtcactc tactgcactg aaaacaaccc gccgtttgat aacggcctac
    65461 tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga tgaacgccag agcaaaacct
    65521 tctggttcga tatccgcaac accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg
    65581 cccagacgca cggcgatcag gggctggcag ccgatcctat caaagcgtac ttcaacggca
    65641 ccgcaggcca gagtttcggt gtgtggaacg cgggcggcgt ggaactatac ctgaccgggg
    65701 atgccaacga ctatgtcggt aaaggcatgg cgggcggctt aatcgccatt catcctccgg
    65761 ttggttccgc cttccgcagc catgaagcaa gcattatcgg caacacctgc ctgtatggcg
    65821 cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact
    65881 ccggtgctat caccgtggta gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg
    65941 gtattgtctg tattctgggt aaaaccggcg ttaacttcgg cgcgggcatg accggcggct
    66001 tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg cgttaacccg gaactggtcg
    66061 aagtcttaag cgtcgacgat ctggcgatcc atgaagagca tctgcgcggt cttatcaccg
    66121 agcatgttca gcataccggt tctcagcgcg gtgaagagat tctggcgaac tggtcaacct
    66181 tcgccacgaa attcgcgctg gttaaaccga agtccagtga tgtaaaagca ctgctgggtc
    66241 accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga
    66301 gtcagaatgt ttatcaattt atcgacctgc agcgcgttga tccgccaaag aaaccgctga
    66361 agatccgtaa aattgagttt gttgaaattt acgagccgtt ttccgaaggc caggccaaag
    66421 cgcaggctga ccgctgtctg tcgtgcggca acccatactg cgagtggaaa tgcccggtac
    66481 acaactacat cccaaactgg ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag
    66541 aactgtcgca tcagaccaac acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc
    66601 gtctgtgcga aggttcctgc accctgaacg atgagttcgg tgcggtgacc atcggcaaca
    66661 ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg gcgtccggat atgtccggcg
    66721 tgaagcaaac cggtaaaaaa gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg
    66781 cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg
    66841 gcgggctgct gaccttcggt attccggcct tcaagctgga aaaagaggta atgacgcgtc
    66901 gccgtgaaat cttcaccggc atgggtattg aattcaaact caataccgaa gtgggccgcg
    66961 acgtacagct ggacgatctg ctgagtgatt acgatgccgt gttccttggc gttgggactt
    67021 atcagtcaat gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc
    67081 cgttccttat cgccaacacc aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt
    67141 tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg tggcgacact gcgatggact
    67201 gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg
    67261 aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt
    67321 tcaaattcaa cgtccagcca ctggggattg aagtgaacgg taacggcaaa gtcagcggcg
    67381 taaaaatggt acgtaccgaa atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga
    67441 tcgttgcagg ttccgaacat atcgttccgg cagatgcggt gatcatggcg tttggtttcc
    67501 gtccacacaa catggaatgg ctggcaaaac acagcgtcga gctggattca caaggccgca
    67561 tcatcgctcc ggaaggcaac gacaacgctt tccagaccag caacccgaaa atctttgctg
    67621 gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag
    67681 cggcagacgg cattatgaac tggctggaag tttaagctct gtcccagcgc cgcctcctca
    67741 gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa tcgcctcgca taatctgaag
    67801 aaaaccaatg taatacgctc atctggattg ttaccggtag atgcccggcg tatagctgat
    67861 tccattattc aacgcagcgc catataaagg gacagataat ggaatcgctc tcagagagaa
    67921 cctcaacagg ctaccagcaa atccacgacg gcattattca tctggtcgat agcgcccgaa
    67981 cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac gtactgggaa attggccgac
    68041 ggattgtcga atttgaacaa ggtggtgagg ccagagcagc ttatggtgca cagttgattg
    68101 atcggctatc gaaggattta agtcgacggt ataaacgtgg ggtctctgca aaaaacttac
    68161 gccaaatgag gcttttttac ctcttttttc aacatgttga aattcgccag acagtgtctg
    68221 gcgaattaac accattggga attccgcaga caccatctgc agaattccca tcggcaaaga
    68281 tttggcagac gctgtctgcc aaattgagag gctgtttgat atttccacag cttttgtatt
    68341 gcatctccta ttttcggttt gtatattcat gcaaataaac ga
//
LOCUS       DAJTYI010000004        67227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYI010000004 DAJTYI010000000
VERSION     DAJTYI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1566)
                     /locus_tag="OO564_000271"
     CDS             complement(<1..1566)
                     /locus_tag="OO564_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6651536.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAAL"
     gene            complement(1550..1906)
                     /locus_tag="OO564_000272"
     CDS             complement(1550..1906)
                     /locus_tag="OO564_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6651537.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2029..2211)
                     /locus_tag="OO564_000273"
     CDS             complement(2029..2211)
                     /locus_tag="OO564_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651538.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2195..2635)
                     /locus_tag="OO564_000274"
     CDS             complement(2195..2635)
                     /locus_tag="OO564_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6651539.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2635..2931)
                     /locus_tag="OO564_000275"
     CDS             complement(2635..2931)
                     /locus_tag="OO564_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6651540.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2928..3266)
                     /locus_tag="OO564_000276"
     CDS             complement(2928..3266)
                     /locus_tag="OO564_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6651541.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3263..4474)
                     /locus_tag="OO564_000277"
     CDS             complement(3263..4474)
                     /locus_tag="OO564_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6651542.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4476..5048)
                     /locus_tag="OO564_000278"
     CDS             complement(4476..5048)
                     /locus_tag="OO564_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6651543.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5088..6245)
                     /locus_tag="OO564_000279"
     CDS             complement(5088..6245)
                     /locus_tag="OO564_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6651544.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6545..6769)
                     /locus_tag="OO564_000280"
     CDS             complement(6545..6769)
                     /locus_tag="OO564_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651545.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6911..7072)
                     /locus_tag="OO564_000281"
     CDS             complement(6911..7072)
                     /locus_tag="OO564_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6651546.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7082..7387)
                     /locus_tag="OO564_000282"
     CDS             complement(7082..7387)
                     /locus_tag="OO564_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651547.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7384..7662)
                     /locus_tag="OO564_000283"
     CDS             complement(7384..7662)
                     /locus_tag="OO564_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651548.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7942..9690)
                     /locus_tag="OO564_000284"
     CDS             complement(7942..9690)
                     /locus_tag="OO564_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6651549.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9687..9986)
                     /locus_tag="OO564_000285"
     CDS             complement(9687..9986)
                     /locus_tag="OO564_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6651550.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9992..10225)
                     /locus_tag="OO564_000286"
     CDS             complement(9992..10225)
                     /locus_tag="OO564_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651551.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10227..10447)
                     /locus_tag="OO564_000287"
                     /pseudo
     CDS             complement(10227..10447)
                     /locus_tag="OO564_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10440..10661)
                     /locus_tag="OO564_000288"
     CDS             complement(10440..10661)
                     /locus_tag="OO564_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651552.1"
                     /translation="MHNKTTPNAAAAALTTLMHAIIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10651..10851)
                     /locus_tag="OO564_000289"
     CDS             complement(10651..10851)
                     /locus_tag="OO564_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6651553.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10860..11606)
                     /locus_tag="OO564_000290"
     CDS             complement(10860..11606)
                     /locus_tag="OO564_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651554.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11664..11852)
                     /locus_tag="OO564_000291"
     CDS             complement(11664..11852)
                     /locus_tag="OO564_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6651555.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12247..13476)
                     /locus_tag="OO564_000292"
     CDS             complement(12247..13476)
                     /locus_tag="OO564_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6651556.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13725..14846
                     /gene="roxA"
                     /locus_tag="OO564_000293"
                     /gene_synonym="ycfD"
     CDS             13725..14846
                     /gene="roxA"
                     /locus_tag="OO564_000293"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6651557.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14895..16121)
                     /gene="pepT"
                     /locus_tag="OO564_000294"
     CDS             complement(14895..16121)
                     /gene="pepT"
                     /locus_tag="OO564_000294"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6651558.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16371..17507
                     /gene="potA"
                     /locus_tag="OO564_000295"
     CDS             16371..17507
                     /gene="potA"
                     /locus_tag="OO564_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6651559.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17491..18348
                     /gene="potB"
                     /locus_tag="OO564_000296"
     CDS             17491..18348
                     /gene="potB"
                     /locus_tag="OO564_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6651560.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18345..19139
                     /gene="potC"
                     /locus_tag="OO564_000297"
     CDS             18345..19139
                     /gene="potC"
                     /locus_tag="OO564_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6651561.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQSFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19136..20182
                     /gene="potD"
                     /locus_tag="OO564_000298"
     CDS             19136..20182
                     /gene="potD"
                     /locus_tag="OO564_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6651562.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20338..21159)
                     /gene="cobB"
                     /locus_tag="OO564_000299"
     CDS             complement(20338..21159)
                     /gene="cobB"
                     /locus_tag="OO564_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6651563.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21175..22086)
                     /gene="nagK"
                     /locus_tag="OO564_000300"
     CDS             complement(21175..22086)
                     /gene="nagK"
                     /locus_tag="OO564_000300"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6651564.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22115..23359)
                     /gene="lolE"
                     /locus_tag="OO564_000301"
     CDS             complement(22115..23359)
                     /gene="lolE"
                     /locus_tag="OO564_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6651565.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23359..24060)
                     /gene="lolD"
                     /locus_tag="OO564_000302"
     CDS             complement(23359..24060)
                     /gene="lolD"
                     /locus_tag="OO564_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6651566.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24053..25252)
                     /gene="lolC"
                     /locus_tag="OO564_000303"
     CDS             complement(24053..25252)
                     /gene="lolC"
                     /locus_tag="OO564_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6651567.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25514..26560
                     /locus_tag="OO564_000304"
     CDS             25514..26560
                     /locus_tag="OO564_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6651568.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26688..30134
                     /gene="mfd"
                     /locus_tag="OO564_000305"
     CDS             26688..30134
                     /gene="mfd"
                     /locus_tag="OO564_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6651569.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30277..31239
                     /gene="ldtC"
                     /locus_tag="OO564_000306"
                     /pseudo
     CDS             30277..31239
                     /gene="ldtC"
                     /locus_tag="OO564_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31322..31579)
                     /gene="bhsA"
                     /locus_tag="OO564_000307"
     CDS             complement(31322..31579)
                     /gene="bhsA"
                     /locus_tag="OO564_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6651570.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31820..32452
                     /gene="comR"
                     /locus_tag="OO564_000308"
     CDS             31820..32452
                     /gene="comR"
                     /locus_tag="OO564_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6651571.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32514..33053)
                     /locus_tag="OO564_000309"
     CDS             complement(32514..33053)
                     /locus_tag="OO564_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6651572.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33280..34584)
                     /gene="ndh"
                     /locus_tag="OO564_000310"
     CDS             complement(33280..34584)
                     /gene="ndh"
                     /locus_tag="OO564_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6651573.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34985..35527)
                     /locus_tag="OO564_000311"
     CDS             complement(34985..35527)
                     /locus_tag="OO564_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6651574.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35550..36575)
                     /gene="nagZ"
                     /locus_tag="OO564_000312"
     CDS             complement(35550..36575)
                     /gene="nagZ"
                     /locus_tag="OO564_000312"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6651575.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36586..37410)
                     /gene="thiK"
                     /locus_tag="OO564_000313"
     CDS             complement(36586..37410)
                     /gene="thiK"
                     /locus_tag="OO564_000313"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6651576.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37391..38032)
                     /gene="lpoB"
                     /locus_tag="OO564_000314"
     CDS             complement(37391..38032)
                     /gene="lpoB"
                     /locus_tag="OO564_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6651577.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38047..38424)
                     /locus_tag="OO564_000315"
     CDS             complement(38047..38424)
                     /locus_tag="OO564_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6651578.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38427..38786)
                     /gene="hinT"
                     /locus_tag="OO564_000316"
     CDS             complement(38427..38786)
                     /gene="hinT"
                     /locus_tag="OO564_000316"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6651579.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39120..39944
                     /locus_tag="OO564_000317"
     CDS             39120..39944
                     /locus_tag="OO564_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6651580.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39948..41309
                     /gene="fhuE"
                     /locus_tag="OO564_000318"
                     /pseudo
     CDS             39948..41309
                     /gene="fhuE"
                     /locus_tag="OO564_000318"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41369..42802)
                     /gene="ptsG"
                     /locus_tag="OO564_000319"
     CDS             complement(41369..42802)
                     /gene="ptsG"
                     /locus_tag="OO564_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6651581.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43097..43894)
                     /locus_tag="OO564_000320"
     CDS             complement(43097..43894)
                     /locus_tag="OO564_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6651582.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43905..44909)
                     /gene="holB"
                     /locus_tag="OO564_000321"
     CDS             complement(43905..44909)
                     /gene="holB"
                     /locus_tag="OO564_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6651583.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44906..45547)
                     /locus_tag="OO564_000322"
     CDS             complement(44906..45547)
                     /locus_tag="OO564_000322"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6651584.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45537..46559)
                     /gene="yceG"
                     /locus_tag="OO564_000323"
     CDS             complement(45537..46559)
                     /gene="yceG"
                     /locus_tag="OO564_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6651585.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46562..47371)
                     /gene="pabC"
                     /locus_tag="OO564_000324"
     CDS             complement(46562..47371)
                     /gene="pabC"
                     /locus_tag="OO564_000324"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6651586.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47491..48732)
                     /gene="fabF"
                     /locus_tag="OO564_000325"
     CDS             complement(47491..48732)
                     /gene="fabF"
                     /locus_tag="OO564_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6651587.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48819..49055)
                     /gene="acpP"
                     /locus_tag="OO564_000326"
     CDS             complement(48819..49055)
                     /gene="acpP"
                     /locus_tag="OO564_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6651588.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49266..50000)
                     /gene="fabG"
                     /locus_tag="OO564_000327"
     CDS             complement(49266..50000)
                     /gene="fabG"
                     /locus_tag="OO564_000327"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6651589.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50013..50942)
                     /gene="fabD"
                     /locus_tag="OO564_000328"
     CDS             complement(50013..50942)
                     /gene="fabD"
                     /locus_tag="OO564_000328"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6651590.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50958..51911)
                     /gene="fabH"
                     /locus_tag="OO564_000329"
     CDS             complement(50958..51911)
                     /gene="fabH"
                     /locus_tag="OO564_000329"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6651591.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51979..53049)
                     /gene="plsX"
                     /locus_tag="OO564_000330"
     CDS             complement(51979..53049)
                     /gene="plsX"
                     /locus_tag="OO564_000330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6651592.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53130..53303)
                     /gene="rpmF"
                     /locus_tag="OO564_000331"
     CDS             complement(53130..53303)
                     /gene="rpmF"
                     /locus_tag="OO564_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6651593.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53355..53876)
                     /gene="yceD"
                     /locus_tag="OO564_000332"
     CDS             complement(53355..53876)
                     /gene="yceD"
                     /locus_tag="OO564_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6651594.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54075..54659
                     /gene="yceF"
                     /locus_tag="OO564_000333"
     CDS             54075..54659
                     /gene="yceF"
                     /locus_tag="OO564_000333"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6651595.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54771..55730)
                     /gene="rluC"
                     /locus_tag="OO564_000334"
     CDS             complement(54771..55730)
                     /gene="rluC"
                     /locus_tag="OO564_000334"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6651596.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56303..59488
                     /gene="rne"
                     /locus_tag="OO564_000335"
     CDS             56303..59488
                     /gene="rne"
                     /locus_tag="OO564_000335"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6651597.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59685..60638)
                     /gene="flgL"
                     /locus_tag="OO564_000336"
     CDS             complement(59685..60638)
                     /gene="flgL"
                     /locus_tag="OO564_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6651598.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60650..62292)
                     /gene="flgK"
                     /locus_tag="OO564_000337"
                     /pseudo
     CDS             complement(60650..62292)
                     /gene="flgK"
                     /locus_tag="OO564_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62358..63299)
                     /gene="flgJ"
                     /locus_tag="OO564_000338"
     CDS             complement(62358..63299)
                     /gene="flgJ"
                     /locus_tag="OO564_000338"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6651599.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63299..64396)
                     /gene="flgI"
                     /locus_tag="OO564_000339"
     CDS             complement(63299..64396)
                     /gene="flgI"
                     /locus_tag="OO564_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6651600.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64408..65106)
                     /gene="flgH"
                     /locus_tag="OO564_000340"
     CDS             complement(64408..65106)
                     /gene="flgH"
                     /locus_tag="OO564_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6651601.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65159..65941)
                     /gene="flgG"
                     /locus_tag="OO564_000341"
     CDS             complement(65159..65941)
                     /gene="flgG"
                     /locus_tag="OO564_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6651602.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66079..66830)
                     /gene="flgF"
                     /locus_tag="OO564_000342"
                     /pseudo
     CDS             complement(66079..66830)
                     /gene="flgF"
                     /locus_tag="OO564_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66850..>67227)
                     /gene="flgE"
                     /locus_tag="OO564_000343"
     CDS             complement(66850..>67227)
                     /gene="flgE"
                     /locus_tag="OO564_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6651603.1"
                     /translation="TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFAN
                     NEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQR
                     NYQSNAQTIKTQDQILNTRVNLR"
BASE COUNT        15918 a        18102 c        16543 g        16664 t
ORIGIN      
        1 cagcgccgcc acgctcgggt ctatcttgtt cggtgatttt ttcttgttcg gcttgatatt
       61 ggcgttcgcg tcactctgca tcacgacgtt actcatcgac caggacaaca ccggatcgcc
      121 acgatgcaca atcaccctgc ggttaacaaa aacttcgaac gatttcgccg ccggactgaa
      181 tctgaggtag gtttgcggga acggctccac ctcaaaacca gccccctgta attgcgtcct
      241 gagatgcgtg gcgttccacg tatcaaagcc caccagcctg atattaaatt tctccgcatc
      301 ctgcatgatg tcatctctga tccggtcgta atcaatgcag tcacctggcg ttgtgcgtat
      361 ccagcccgct ttagcccact ggcgatagac agcgcggttt ttattggcgg ggttctgtag
      421 ctggaactcc ggcagataat gacgggaaat cagcataatg gttttaccga ccggaaaggc
      481 atagcatacg ctggaaatat cgctggttga tgataagtcc agccccgcgt aacactcctg
      541 accgtgtaaa tcttcctccg tgaacgttcc ggcacactca gcccatgcac cattacccat
      601 ccacggggta gtcccctgac accagatatt aaatcgcttt gtcatcatct ccacccactg
      661 cgacggaata ccccgcgctt tctggatggt tgaggccagt ttttcacgat ccacggaaac
      721 atcaatatta gggttcgcct ttatccacat cgccggatca tcaacctcgt ttttgtcgtc
      781 cagttcgtaa atcagtacaa aaattgaatc gttgacctct tcgccgtcca ggatctggca
      841 gcaataatca tagtgctgtt tacaggctga aacgacgtta ctgcccgatg tggtaatggc
      901 aaataacagc ccttcgggac gtgcgcccat ccccagctca agcgcggaat aaacgccgtt
      961 atcggggtgt aggtggtatt cgtccacgat ggaaagactg gggtttgtcc cctcgatggt
     1021 tgccgctttt gctgccagcg gctttaacag gctgttgctt ttcgggtgta tcaccttatg
     1081 cgcctgaata ttcacccgcc tgcgtaacgg tcgggataaa aggcacatct gacgcgcatc
     1141 atcaaacacg atccgcgcct ggtcacggct caccgctgcg gtgtagatat cctgctgacc
     1201 gttctccata atcagaaacc agttagccag aatcgcggcg gtcgtggatt tcgcattttt
     1261 gcgcggcact tcgataaagg cgctcgtgta tttgcgccgt ccggtggcct taaccttaaa
     1321 gccgaggatg cacgcaaagg cgaactgctg ccacggctcc agctcaatgg gtctgccacg
     1381 catcggtcct tttacgtgcg ggcacaccct ggaaaaggca ataaaccgct ccacaacctc
     1441 acgatcgaac atgtaaaggg ggcttttaag gtccgaaaag taccgtttaa cagcctgttt
     1501 taaccgttta caggccggaa ttttgcccgt ttttacgtct tctgcgtact tattccaggc
     1561 ggtcaagctc gtcctcttct tctgtttccg gtggattttt acggcggctt atcgggtcaa
     1621 aaccgagcaa ggaggccatt tttatcatca ctctttcagc gtcggatttt gcgcttaatg
     1681 cggggtttct gctctcgccg ccctgactgt taacaatgct gaacccgcgc gccgcaaggt
     1741 ctgcaacggc tttccggtaa atggagtagt tgacgcaata cagttccaga ttgctccagt
     1801 cggcgggggt caggtctccc cgttccgcaa gctgccgcga tttttcccgc cactgcttca
     1861 cggcgatatc atccaggtag gcgggggctt tcggtggtct tgccatgctt atttttcgtc
     1921 agattatttt tcaaaaaatt cccgtgcata aaaatttggg gaggcgttcg gtgcccggcg
     1981 gggtcgggtt tgtcctgaaa acgcccccca ccccgtcata cagcctcatc agcgattgcg
     2041 gaaacattcc ataacctcgc ggtcacggtc ggttaatcgc ttcgctgtga tgcgttctgc
     2101 gcgtcctgac gctttatctt tatgccctgt ttcctgtgtc ttccatgcgt cacgctgcct
     2161 tataagtcca cggataaggc ggttttgttc ccgctcattc atcagcgtca tacatccagt
     2221 tattgcggtt agcagcccgt tcttcctctt cacgaaatcc acctgcggca cgcttgcttt
     2281 ttgttgccgg atcaagccat ttcgttttct ggttatgaca cgcctggcac aatggttgat
     2341 ggttccattc gggccagaag agaacatcat caccgccatt aatcgggata atgtgatcca
     2401 ccaccacggc gggcgtatat atccccttct caaggcatcg cacgcataac gggtttttac
     2461 tcagatacat ggcgcggtat ttgtcccact gtctggaata tccacgcgcg cggcggtgtc
     2521 ctcgtctggc atcctctgca cgccatgccg cccgcctgtg ctcctcacac ttgccggatt
     2581 taacgcgcct gttacagcct ggttctgtgc atcgtcttaa tggttgccac ggcatcagta
     2641 cacccccaca tcacggtaag ccgtccagag tgcgcctatc gtcatgggta cgcgtgtttt
     2701 tgcgttatcc gcgacaatct ggcgattttc atacaggtga gcgataaaca tcatgcagcc
     2761 aatctttatg gctggcgtga acgccagccc gtcatcaaag cgcctgccta tgtgtatctg
     2821 gcacgcctca agcgatgcgg caatgtatcc gctgattaac tggtcttcat cgtcgccatc
     2881 gatgcggcaa tggagtttca cttcttccag ggtgataagt tcttctgtca tttttccgcg
     2941 ccctcacgac aaagaatttc aaggcgtgtc ctggcggcat ccggcagcgg ctgcccgatg
     3001 atattcagta cacgccccgc cagcggcccc gtactgacct ttatccggct ggtggcgttg
     3061 atgtccttcc ggtaccgtat ccagatcctt acggtaccaa tcgccagctc tgcgcccgat
     3121 gaaatggcct ccttgctgct gaccatgttc acgcttgccc agagtgtgtg accgtcctcc
     3181 cacgtttcga gtatttcgcc ggttatatcc ctggtctgtt tcagggtctg aatcgttatc
     3241 ctgtcacgca gtcgccctat gttcattccg gtttttctcc ctcgctgatt tttacttcct
     3301 gtttccatgc ctggctgaac tcatcaccac catcacgcgg tgacagccct tcacgttcgc
     3361 gggcttcgtt cggacacata acgccggatt ttatgccgcg ctcataggtg gcaaagcgtt
     3421 cgccaggtgt ggcccgtaac aggtccgcgc tgtcaaactc cacctgatac cggataccag
     3481 gtacaggcga ggccaccagc aaggcatttt ttatctgctg ctcaaagttc gccagccacg
     3541 ggcgcatcgt catggtgaga aatgcgcggc ttgcctcgct gaagttgctg taggtgctgt
     3601 tgctgtattc ctgcaaaaaa atcggcgaga cgttgaacat tcgggcgatg tcttcaatgg
     3661 tgaagcgacg ggaggccagc cattcggcat cctgattact catgcccagc tgctgatagc
     3721 tcatcccccc ttcaaggatg ggcgtttttc cggcgtttct ggcccctttg tagcgttcca
     3781 gtgcggctaa tgcctgtttg cccttcacgc cgtccagcca ttcgcctgac gtgataaccc
     3841 ctgccgccat catgccatca cgcatcacgc tcgcgccgtg gcgttgttgg gccagcccaa
     3901 gccccagcga ttcgcggcag atggttacag gtgagcgccc cataaaaccg tcatccgtgg
     3961 agtaacgaag gtggagaacc tcccacggta aatagttgcg ggtgtttccg gtgtaggcgt
     4021 cagtgatgca gtagcgccag ttatgatcac ctgtctgctc cacgttcacc gactgcggcg
     4081 ggtaaggatg taaagccgcc ggaaaaccat cacgccccca ctgaataacc gcataagcat
     4141 taccacttaa caggcagtgg cggatcatca ttcgcttaaa ctggtagggt gtctgccacg
     4201 cgttcgggcg ctcgttgagg atgtgatcga ccggatgaga atcaagccat tcgcgggcct
     4261 ccttcccctt ctcattgcgt accaggtaca ggtaacacgg catggtagcc accgcctcag
     4321 agatgaccgt gacggcgttc atgacggcgg agagtgattc cgctgtccca gatgatacgt
     4381 actcacccgc ccccgtgttc gatgtgccgg ccagcgccat aaattcatca agcgtcatgc
     4441 tgcgctgctc tttttttcgt ctgaaaggcc acatatcaca cccccgctaa atccgcccac
     4501 cagcggcgat tatcctcacg cggcattttt tcggggtgct gctcatacag ggaacggcgg
     4561 gccagctcca cgccggaatc ggggtaagcc ggtaccgatg taacggtaat ttcgtacagt
     4621 tccgccacca gcacggtgcg cacgcatgga tctgttgtgg tatcccatac atccttacgg
     4681 gagcgaaagc taaagctcat ccccgagatg tccccacgct taaccagttc gataacgtcg
     4741 cgccctgtac tggtatccgg tggggtcagt tcaaagcgta accctgtttc gtcctcttcc
     4801 agtttcagcg tgccggaacg ggtgcgcccc agtaacatgc tgtggtcatg ctcatacagg
     4861 ccgcgaacgt cattacccgc cgcaagccat tcagtaaacg ccccccgctg gaatttttcg
     4921 taaaactcac cccataacag ttctgaaagc ttatcccagc gaacaacgta gcccgtcagc
     4981 gtaccggctc cgctggtggt gatttccgat gaccggattt ccatactctt cataattttt
     5041 tcacccataa aacactaaag gggcttttta gccccttctg tatgctgtta atcgtcgtct
     5101 tccggcagtt ccagaatctt gatcgcgttc gaatccacca cgccaccgcc taaatatttt
     5161 tgcgtgaaaa ttttgatgaa tcctggttct gtcaggttgt ccggtctggt gcgaacacct
     5221 gtttcgtgat caacaatgta gtaaccgcgt ttgaagtcac ccagggcaat aacgttatca
     5281 ggcataaact ccagatattc gaccggaagg cccagcaacg tatcaggatc acccgcctgt
     5341 aaacggtcgc gccagatata atcaccgttc gcgttcttca ccttctgaag ttttgccgcc
     5401 gtcgtggagt taaccaccca gaccgcgttt ttgcggtatt ttttacgtaa tgcaaatttc
     5461 aggtcgatca gcgggtccgc agatgtccac gccagagatt cggaaggttt aattacctgc
     5521 aacgtaccaa aatcacgctc tttgtcgttc ttctctgcac ggggtacgga taaaaaacct
     5581 tttgcttttt tgtcaccgtc gcccacaacc agatcgcttt cttcggtttc cgtgaaggtg
     5641 tcgccaatct cacccgtcag ccatgaaagg atgtccacat cggaaaaatc cacgatttcc
     5701 tgtgtggtgc gcgggtacgc atagaccgga tacagcttaa tgctcacctc gttaatctgc
     5761 ggggtgctgg tctgttcgcg tgccttaccc tcttcaccgt ggttaacggt cgcaccgcca
     5821 gcggaaacaa gctgcttaaa ctcgttgctg ctgattttct tcacggtaca gatgcggcgc
     5881 atagtggatt catccgccag cattcgcatg atttcggtgt tcagttcagg gataacggta
     5941 taaccaccat cagcgggaac gcctgtactt aatgcgcggg tttcaccggt cagaatgtag
     6001 ttgcgtagtt ctgcggggtc agtggtctgg ctgcttttac ctggcttgct gcgctcttcg
     6061 tctgcaatgg cttcaaggcg ggaaatgtct ttatcgaggg attcagcttt agcgcgtaat
     6121 tcgtcaaatt ttgcgccctc agcatcgtta agactgcggt tttctttttc agcgttctcc
     6181 agcatgtcgc gcatctgatt tttaatggcg gttttctgct ggcgtaattc gattattctc
     6241 tgcataaaaa agtcctgggg ttaagtaagg aactccagga cgcggcaaac actcaaccgt
     6301 ttttcataag gaaatcagta atcgcaccga tcgttttccc gcctggtaat gaatattggc
     6361 gagcacatta acaggcggga aagtggcccc agcgtcctgg caccacgggc gagaataatc
     6421 atgattcagt tcgggtaaaa tatgccgatc cgctcagtga acaacgtgga acaaccacga
     6481 acaaataatt tacaaaaaat aacaaaaagc cggattgctc cggctgtatg tgctgtatgg
     6541 gtgctcagtc tctgatccct ttcagaaatt ctatcaatgc atctgtctgt tcaggattta
     6601 ctgccagcat ttcaccggat agcgcgcaac gaacaaaacc atgatgatcc ttttcaatca
     6661 gcgcccccgt ttccagaaat gcgcggtaat cagtgatatt catcgtctcc atgttgtcag
     6721 catggtattt atcacgctgt ttaagtatct catcaaattt catcggcatt gcgttttcct
     6781 ctgttgtgtc tgtttgtttc agatagtaac tatgccggac cgtgacgaaa agccagtagt
     6841 gctccatacc gtttcaaatg acgcaaaaaa agccggattg ctccggcttc gtggtgtggt
     6901 tctgatattc ctactctgct aatttgctca gccccatgtc tgcgtaagtg cgatttactg
     6961 catttctcag gtctgcgtag ttctccgttg gcggctccgg tgtgcccttg ctgctctgtt
     7021 ttaccagcgg gtcgcccacc agccgcccgt aagctgcaat ttgtgctgtc atattgccac
     7081 ctcagtgaag cgatccggca ttgttctttt tcatctcttc catcattttt tcagtgagca
     7141 ttacgcactg aaaccccatg tttttgatgt ctacgggttt catgcctttg tcagtggata
     7201 tcacaggcag aatatcctga tacacatcgc ggtcagggta actaagcaca caaagcgccc
     7261 atgatgccac gggtgaggat ccttcgtctg tttcaaccca ccagcctggt gtagctgcgc
     7321 ttatttgcag aatcgtgatc cgtccgttgt gcttgtaaaa tttttcacca aattttggtt
     7381 cgctcatgat tccacctctc cggttttaac gttgattgtt gttacctgtt ccgcttcggc
     7441 aatctcccgc tctgtcagcg tggcaaaatt tgccgccgtc gtggtcatga atgcgcttat
     7501 cagttcggga tgtgctttcg cgtatccttc ccccgcgtgg cggtctatcg ttctgattgc
     7561 cacctttaaa gcgtgctctg tcatgtctaa cgcgcgatat ttcggttctg ttctgtctct
     7621 gcgttttttg agtgatttat taaatttccc tgaagtgtgc atatttattt ttaccccctc
     7681 gtttaaaaag ttttgagttg tgcctcccct tgtctacctt agtggccctc atgccagtaa
     7741 tggcgctgct ttcagcgggg tagagtgctt ttatccacta tctaccccgt gtctacctcc
     7801 ctgtctgatt caggtaaaat caggtagaga gggtagatag tgggtagaca gtaaaaaaag
     7861 gctacctacc taacttaatg cactgaatta aatgtatttt tctttactca ggtagatagg
     7921 gtagacagca attacaaaaa attataaaaa cgcgtcgcac tcgtctgttg ttattgcgtt
     7981 agtctgcgtt actcccttaa ctttccgcgt aatatattca tgtccgtaaa ctttcgcggc
     8041 tggcttcata gccttgccaa agtcatttac gtttagcggt ttgctcctgc ctgcgtacgc
     8101 cataaacgcc agatagacgc ggtaaaggct gtttctggtc gtgtacttca ctgaatcgcc
     8161 accgccgccc atcatcaggc cgcgtgcttc ctccagaaaa ttcaggaact ggcaaaactc
     8221 aataaccgga tccgtctgtt gctttattgc cagagcttca tcaccgtcac gctgttccag
     8281 tagtaaagcc cgtgctttct cagagtcggt aaagttcgcc agcaatcggc ggataatgac
     8341 agggatttca gccgcaatct tttccggtag ctccctgtct ttttcggcct cactaacgat
     8401 attgtcgaaa cggaaaatca cgcgacgacg tgccacacct ccggcccgtt cggtgaatat
     8461 catcgggtta ttgttggtcg ccagcaccac cgccctgatt acagccgtga aacgcttttc
     8521 gtatttcggg ttaatttcca cggggtcacc gcccgtgatt ttcttgatgc ccgttccttc
     8581 gcctgtatat ttcggctggt cagccaggac gataagacga ctcccgacaa cctgcggacg
     8641 tccaccagca tcatcaagcg atgtcatttc agcgcttacc gtgttctgtt tccctgccag
     8701 aaggctggct atgtgtgtga atgtactttt accgctcccg ccgtctccgg tggcctcaat
     8761 aaacatctgc cagtcgtacc ggttcgccat aatcatgtac agcgcggcac atatacgcat
     8821 catcttgcgc tggtcttttc cggctgcgtg ctcaagccat ttatgaaagt ttagcgcgtt
     8881 atcgcggatg ttctcccctg gtgctggtgg cgtgtactca atgccgttgt gcgtggtgat
     8941 ccagttctcc ggcgtgtgcg gggaaaattc ccccgttttc aggtcaagcg caccattggc
     9001 gaacggcagc aaatcgccgg acggctcgcc cattggttcg gcaataactt ttaacgcttc
     9061 cacggcgtta ttgattacgc gcttgctgaa agtggccctg tgctctgaat agatcgccac
     9121 catttcgcgg ctaagttcca ttgtgctgac cggacaccat accccgccgc gccatacgtg
     9181 aacgatttca ctttcctgat gcacacaaac gccatcaaag cgctcggcaa gcagctgcgc
     9241 gcgctcactg tctgccattt gtgccagttg cgtatttttc tgctccggca tggtaagccc
     9301 tgcggcaata ttttcacgct cagcattcag atagcgccgc cagttttcac acttctggac
     9361 gtgcattcct tcggggtaaa aatttgcgtc ctgaatatct gccgccgcca gcttctgacc
     9421 aatctttttg gtctccacca aatccagttc tccggcctgg tacagcctta cgcgtttttt
     9481 tccatccgga acaattttca gtgcatcaag ttcggcaagc tgatttggcc caagccacac
     9541 aggcggcaca ttatcgccgg atgcggggcc atcctgctcc tgccattgtt tcgcgtgcga
     9601 ccaggcatca ctacccgcaa agataattac ctctgtgtct ttgtgtttta ttccgcgtgg
     9661 ctgttttttt acgttcggtg ccagtttcat tttttacccc tgaatacgtt aagcatcttt
     9721 tttatttcct gaatattggc gcgtgctttc tccctgctgg ttggtttact gcggggcgct
     9781 gcctgtacca gcgtgaaatc acgccggaat tgataaacag gcatcacgca atcatattcg
     9841 taaccttcac ggcggtaggt gatgcgccgt tctgccacgc ctttaatcgt taccatgccg
     9901 ccgtactggt cgcggtaaat atcaccgcgc gtaaatttag ggtgagtgtt gccactggca
     9961 gttaagccag aatatttaag tttcattatt tttattctcc ggtgtgctgt tctttatatc
    10021 tgtcgtgcaa tagatctatt tcttgcagtt ccattattac aggctcaaga agcgttatta
    10081 atgccgtggc aattcttgat ttttgtttgt cgcgttcatt gtcgccaagt gtttcaagcc
    10141 atatgcgcaa tatttccagc atgttttcac tgtgagaaag tgcaagaaat gcgcggtcta
    10201 ttgtttcgtg gtaaatatca cgcatattaa tccccgtccg tcgtttttct taaaataacc
    10261 tctgtcacga aatcagcata atcggcggcg atattcagta catcaagccc gttgatttat
    10321 attctctcgt ggaaagtaag aaaaaagccg ctctaataag ttctggcatt gacgaaagcg
    10381 catcagccgc atcatcagga acgccggaaa attcctgttt cagggaatta aaacgatcat
    10441 cacgcataac cccccccatt ttcacaatcg gcaacaagaa tatttttagc gtcattcagt
    10501 gaccgcgttg cgatgtatcg gatacattcc agggcgaact gtgtccggtc tttctgttct
    10561 tctccctgcg caagctctgc cgtgcattca atatcaataa tcgcgtgcat cagcgtagtg
    10621 agtgcggcgg ctgctgcgtt cggtgtggtt ttattgtgca taggtttccc cctgacgaat
    10681 acgggcggcg aatacagcaa cacaaccgga cgggcaacgg ctacgcgctt cgcgttccgt
    10741 ccaagcggtt acgtggatga tttgggattc tccggtactc agtgccagaa aacgccacac
    10801 aaaggccgtt tgtgtgtgta caaggtgtgg tatatgattt acggcaacca taacggctcc
    10861 tagtttacgt tgttggttag aagccccgtt actgctccta cagtgcgggg ctttgctttt
    10921 tgtgttgatt gtgtgtaatg tgtcattaca cataaataca ttacatgagg tgtaattaac
    10981 gtgtcaacac acaaaaacga aaggcgtggt aatcctccgt tccagttccg tttagatcct
    11041 gacttacggg aaaagatgga aaaggcacaa caacaggacg gcgatgattc tcttgctgcc
    11101 tggataaagc gaataatccg caaagaatta cagcaacgcg gcatagaacc caaaggataa
    11161 aaactatcag cgccgtggtg tgaggtacta cggcgcattg ctttacaggc gcacacaatg
    11221 accaacgaag aatcaaccga aaaaacatca cctgcacgaa aaagacgacg cagaaagaaa
    11281 gagcatgaat cagaacgatt cgcgccgtgt tcttttgccc ttgagaagta ccttaaagaa
    11341 tactcatcaa aggagagaac ttcgcagtta tggcaacgca ctgaaccaga ctgattgcat
    11401 tgcccaccag cctgatagcg gctatcatcc ccgtgcttat gtttgggatc acacacacaa
    11461 acggcgcagc gggttatctg ttcagaaagg cggctccatt tcggggccgc tttttttatg
    11521 cctgaaaaac cccaattttg tggttttcca gtttcaccag ggcgaacgaa tccccgccca
    11581 cgttctggcg tatattcaat cttcatggtt atagctctgt gttcagatga ttgatgtgtg
    11641 gcggctgcgt gccgccagcg tgattaatga actgccttgc agctatcctt ccaggccaga
    11701 acctcggata aagaccagcc aacggaacga ccgccaagtt tacgacgtga tgggaattgt
    11761 ccggcctttt ccaggcggta gcggcatgag cggctaaggc ctgttagctt ttcgcattct
    11821 ttttcacgta taaaccgatc agtgcttaac actattgccc cctttcgttt cttaaagagt
    11881 tatttcgtgt tctattgcgt tgggatgtgt ctgattgtgt caggatgatt cagagttggc
    11941 aaatgttgag gccgtatggt ttacagaaag aggaataatc aggataaaat catttaaatt
    12001 catgttaata caaaggcata aaatattgtt tcatgccttt tttctcgcta tttaaagagt
    12061 gattcgctag tgtataaaaa accagtaaga cgttaaaaat cagctactta taaatctgta
    12121 cactttttca cctcttgttc gtagttgttc cgcattgttg ctcattgttg ctcattgttg
    12181 ctcattgttg cacgttgtat ctgttcgcat atccagtatg cgcatactga aaaaacacga
    12241 aaaaaattat tttcttctgg ctactggtag cgtggttacg ttttcatgtg ttcccgccag
    12301 tatccctaac cgctccgtcc acatatccag cgcattgcgt ttagcatcca gataacggga
    12361 atgattataa actcgctgca ttcctggcat ctggtggcct gtaagctgct ccacgacgtg
    12421 aggatccacg cctaaatcgt tcagcatcgt tgtaaaggtg cgccggatgt catgtagtga
    12481 ccagtgaggg tgatttagcc tcctgtgcgc taatctgccg tactgcgaca cgcttgtttc
    12541 ctgtttcact tcccccagca ataagcccgt gtgcctgttc tgctccacca gctgcgtgac
    12601 gaacggcagt attgcttccg gtatgggccg gaatattgcc accttcgttt tgctgtgttc
    12661 tttcggcacg gtccagagca tttcggtaaa atcccactcg ctgatctccg ataacctcag
    12721 ttctaccgtc cggcatccga acacaatcag gaggcggatt aacgcgatgt agtaggggga
    12781 gaatattttt ttgtccagtg cctgcaataa ttcgcccagt tctttggtgc ttaagacacg
    12841 ctcgcttata tccggttttt tcccaacgtc cgccacactc atatcatcaa gaacgttgct
    12901 gattgcatag cgccgcctcc ggcagaactt aagcgcctgt ttgcacgtct gtagcaagaa
    12961 tccggcagta acaggcgttc gctttgccac ctggtcaaaa caggccagcc agtgccgtag
    13021 ctcgcattta tccagcggca tagcaccaat gtgctgtatt acgtgattat taaggcgctt
    13081 tttcagggcg gcataatcca cgcggttttc ctttgcgtac gactcaagcc agtaggtgag
    13141 cgcatcgcca accgttaccg gctttaacgc ttcctgtacg gtgtaattaa gctcatgacg
    13201 tggatttttc ccctctgcca gccatgcgcg acactgggcg gctttttccc tggctgattt
    13261 caggctcaga tcaggataat ttcccagctt aatgcgttcc ggtggtgccc cccttcccgt
    13321 tccggccctg taagtgaaat accaggttaa aaggccactg gttgaatgcc tgacgctcag
    13381 gtttccaccg tcattaagaa aggctgtttt ttgggcgggt gtgccgttga ttttcctcag
    13441 ctgtgtatcg ctcagtttgt taagtgctct gctcataatt ttgattccgg tcatcacaat
    13501 taactacacc attaactaca ccgatcgttg cacaaagggc tacaacgtga aacaagctgg
    13561 aacaaggaaa atcacaaatc tgtttataat caaaaacata atgaacaatc tgaaacatta
    13621 tgaaacggca aaaaacacta attgataaaa tatgtccata cttcacgcat tacgttaagc
    13681 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
    13741 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
    13801 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
    13861 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
    13921 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
    13981 taaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
    14041 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
    14101 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
    14161 aaaagctgca aatgaaacag cactgcccac atccagatct gttacaggtc gatccgttcg
    14221 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
    14281 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggtttt cgcgcgccaa
    14341 atacgcggga attaattagc ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
    14401 actactacag cgatccggat gttccacctc gcgctcatcc tgcggatgtt ctgccgcaag
    14461 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
    14521 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
    14581 cgccttatca gccagatgaa atctacgatg cactgaaaca aggtgatgtg ctggtgcgcc
    14641 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
    14701 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
    14761 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
    14821 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcagc atcacatgcc
    14881 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
    14941 accgcttttt ccataccttc cagaatcaca aactcatgct taccatgata gttgtaaccg
    15001 ccagtgaaca ggttcgggca aggtaatccc ataaacgaca actgcgcgcc gttggtaccg
    15061 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcatcgcctg ctgggcgata
    15121 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
    15181 accaattcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
    15241 attttacgtt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
    15301 tcggcccgtt caacggtgcc tttcatgctc gccaggtgat agaaaccttc atagccttct
    15361 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    15421 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    15481 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    15541 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    15601 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    15661 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    15721 ttaccatcgg tggtaatcaa cgtctgaccc agtagctgat gcagcaccgg gaacataacc
    15781 ggtgataaaa cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca
    15841 atttgcggat taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag
    15901 ccaatcgccg ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc
    15961 tcacttaagg tcacattgat aagccccatt tcttcgagct gctctttcag cagatgcaat
    16021 aacttccatt ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc
    16081 agagacacgt agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt
    16141 gtgacaacat tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct
    16201 gcaagcggga atatttatca gcattacctt aatgaatata aagctaagcc agtaattcac
    16261 aacaaagatt ggcgattcaa gcggtttgcc gtagaattac cgccctcatt aagagtcacc
    16321 aaggtggtta accacaaacc ccgcatcggt aagccatccg ttgcgtttac atgggacaga
    16381 gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc
    16441 gcaaatgctt tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg
    16501 agttcctcac gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg
    16561 caggtctgga aactgttgat tccggacgca tcatgctgga taacgaggac atcacccacg
    16621 ttccggcgga aaaccgctat gtgaacactg ttttccaaag ctatgcactt ttcccccaca
    16681 tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca aaaaaccccc gctgctgaaa
    16741 ttacgccccg cgtgatggaa gccctgcgga tggtgcagct ggaaaccttc gctcaacgta
    16801 aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc cattgctcgc gcggtggtta
    16861 acaagcctcg tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgta
    16921 agcaaatgca gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgttttcg
    16981 tgactcacga ccaggaagaa gcactcacca tgtcggacag gattgtggtg atgcgcgatg
    17041 gtcgcattga gcaagacggc acgccgcgtg aaatctacga agagccgaaa aacctgtttg
    17101 ttgccgggtt cattggcgaa attaatatgt ttaacgccac ggtgatcgag cgactcgacg
    17161 agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt aacttcgccg
    17221 ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga
    17281 ttaacgacga caaccacgct gaagggctga ttggttacgt tcgtgagcgt aactacaaag
    17341 gcatgacgct ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat
    17401 tcttcaatga agacgatcct gactttgacc actctctcga ccaaaaaatg gccattaatt
    17461 gggtagaaag ctgggaggtc gtactggctg atgaagaaca caagtaagtt ccagaatgta
    17521 gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct ttctgcccaa cctgatgatc
    17581 attggcacca gctttttgac ccgcgacgac gccagtttcg tcaaaatggt ctttacgctg
    17641 gataactaca cgcgtctgct cgatccgctc tattttgaag tgctattgca ctcgctgaat
    17701 atggcgctga tcgccaccct cgcctgcctg gtgctgggct atccatttgc ctggtttctg
    17761 gcgaagctgc cacacaaggt gcgtccgctg ctgctgtttc tgctgattgt tccgttctgg
    17821 accaactcat tgattcgtat ctacgggctg aaaattttcc tcagcaccaa aggctatctc
    17881 aacgagtttt tgctctggct gggcgttatc gacacaccaa tccgtatcat gttcacgccc
    17941 agtgcggtga ttatcggtct ggtttacatt ctgctgccgt ttatggtgat gccgctgtac
    18001 tccagtatcg aaaaactgga taagccgttg cttgaggcgg cgcgcgatct cggtgccagc
    18061 aagttacaga cttttatccg catcattatt ccactgacga tgccggggat tattgccgga
    18121 tgtctgctgg tgatgctgcc agcgatgggc ctgttctatg tgtccgacct gatgggcggt
    18181 gcgaaaaacc tgctgatcgg taacgtcatc aaggtacagt tcctcaatat tcgtgactgg
    18241 ccgtttggtg cagccaccag cattacgctg actatcgtaa tgggcctgat gttgctggtt
    18301 tactggcgcg cttctcgttt gctgaataag aaggtggaac tcgaatgatc ggtcgactgc
    18361 ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc attattttga
    18421 ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagagtttt accaccaaat
    18481 ggtatagcct gctgatgaac aacgatagcc tgttacaggc agcgcagcat tcactaacaa
    18541 tggcggtgtt ttcggcgacg tttgctacgc ttatcggttc actgacggca gttgcgctgt
    18601 accgttatcg ctttcgcggt aagccgttcg ttagcggaat gctgtttgtg gtaatgatgt
    18661 caccagatat cgtgatggca atttcgctgc tggtgctgtt tatgctgctg ggtattcagc
    18721 ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccattt gtggtggtga
    18781 cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct ggaagcggcg aaagatctcg
    18841 gtgccagcga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg ccagcggtgg
    18901 cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtagtt tcttcattcg
    18961 tcaccggacc aagttatgaa attctgccgt taaaaattta ttcgatggtc aaagtcggcg
    19021 tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg gtgatggtta
    19081 ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac gttaaatgaa
    19141 aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga gcgccgctca
    19201 cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc cgccaggact
    19261 gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt acgagtcgaa
    19321 cgaaaccatg tacgccaagc tgaaaacgta caaagacggt gcctatgacc tggtggttcc
    19381 ttcaacctat tacgtcgata aaatgcgtaa agaggggatg atccagaaga tcgacaagtc
    19441 gaagttaacg aacttcagca atctcgatcc agacatgctc aacaagcctt ttgacccgaa
    19501 taacgactac tccattccgt atatctgggg tgcgacggcg attggtgtta acggtgatgc
    19561 agtggatccg aaatctgtca ccagctgggc cgatctgtgg aagccagagt acaaaggcag
    19621 cctgctgttg accgacgatg cccgtgaagt gttccagatg gcgctgcgta agctgggcta
    19681 ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc tgaaaaaact
    19741 gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca tggaaggcga
    19801 agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg cgggtacgcc
    19861 tattgacgtg gtgtggccga aagaaggcgg catattctgg atggacagcc tggcgatccc
    19921 ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc tgcgcccgga
    19981 tgtggcaaaa caggttgctg aaactatcgg ttatccaacg ccaaaccttg cggcgcgtaa
    20041 gctgttaagt ccagaagtgg caaacgataa gacgctctac ccggatgctg aaaccattaa
    20101 aaatggcgaa tggcagaatg acgttggcgc agccagcagc atttatgaag agtattatca
    20161 gaagctgaaa gcaggacgtt aattcaacaa gcggacggtg gtattaccgc ccgtttttta
    20221 gttagcaagg atgtggctaa tgagtcagga cagtaaagtt ttatcccgct ctttcgtcgg
    20281 tattgtggtc cgcttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta
    20341 taatccctcc agcaactttt caacaaattc tggcaccacc tggcttgccg ggccgtaata
    20401 tttctcggca aattcattac caacctgact cggttcaaga ttcagttcaa cggtgtgagc
    20461 accatgtagt ttggcttcgt gtacaaaccc agcagccgga tagacatgcc cggaagtacc
    20521 aatggcaata aagacatccg ccatcgacaa cgccatataa atttcatcca tgccgagtgg
    20581 catttcgcca aaccaaacga cgtgcggacg caagggtgcc gggaagtggc aacaatggca
    20641 tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg
    20701 aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag
    20761 gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agagcgtctt gcagtttagc
    20821 cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc
    20881 gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc
    20941 ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc
    21001 cgcagaaatt cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac
    21061 cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcgac gtttattttt
    21121 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
    21181 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc
    21241 aatgcgcgga acacgagcta caggtaagag atgacaaggc aacctgtccg ccagttgcgt
    21301 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
    21361 ggtcaggata tttcccagag aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
    21421 tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg
    21481 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc
    21541 atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc
    21601 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttaccag taatcggttt
    21661 gccgttgaaa accagcccgc cgccaacgcc ggtgccgaga attaacccca tcaccagtgg
    21721 atattgcgta aattcatcct cccaggcttc tgaaagggca aaacagttgg catcgttatc
    21781 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt tgccgctggc
    21841 agcggggaca ttagcggcat acagcttacc atcttctgtt tccggcattc ccggaatacc
    21901 gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac
    21961 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
    22021 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
    22081 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
    22141 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
    22201 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aagtcaataa
    22261 aatagatatc gctggagagg aactgatgac cgatcagctt ttcaatccac tcaataatcg
    22321 gggtaagttg cagtgaaaca actacgccga taaccacccc acacaggctg ccgaacagcc
    22381 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
    22441 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
    22501 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
    22561 tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc
    22621 tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
    22681 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
    22741 ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg gcaatgtgca
    22801 aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg atcatgatcg
    22861 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
    22921 gctgttcgcc cgctttaaaa ttgcgccacg catccccctg aacaaacgag ggtaatgcgc
    22981 tcagacgctg ttcctgttgt gggttaacgc ccttcacctg gattgcgcgc agattcgctc
    23041 cactttccac cagaccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt
    23101 tctgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcttcgattt
    23161 cgccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag ccgttcatcg
    23221 cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag
    23281 agattagcga caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaatcaata
    23341 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
    23401 atcacgcatt tccagttggc ggctcatacg tttcgccagt tgcaggtcgt gagtgaccac
    23461 caggaaagcg gtgccctgca agcgattcaa ctctccaagc aactggaaga tgctgtcggc
    23521 gttacgcgca tcgaggttac cggtaggttc atccgccagt accagacgcg gattattgac
    23581 cagcgcacgg gcaatcgcta cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
    23641 attcgcacga tgctccagcc ccaccgcttt taacatctcc agtgcacggc tgttgatttc
    23701 agcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg cagtaaaatc
    23761 cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca gttctgcttt
    23821 cgccgctgaa gacagtttgc tcatcggctg accgttaaag atcacatcgc cggaggttgg
    23881 cgtatccagc ccgcctaaca ggtgcagcaa ggtactttta ccggaaccag agctaccgac
    23941 aattgccatc atttcacctt cgcccacgct gaaactgaca ttgtgcagca catcggtttg
    24001 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
    24061 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
    24121 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
    24181 ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc
    24241 tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg gcgctggccc
    24301 cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
    24361 cgacttcgcc ctgcttctcc atcaccatca gccccagcga ggtaataatg ttaaacgccg
    24421 caacagcgac aatcaggctc agcagtaagc ccatcatatt tttttccatg cgtacggcct
    24481 ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca ggcagttttt
    24541 gctgacttaa agagtcaact ttcagcggct catccagcca caaacgccag ccggtaatat
    24601 tggctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcatagc
    24661 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa
    24721 tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt tgatcaccgc
    24781 gattgacgcc caactgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
    24841 cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcgccgggt
    24901 cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
    24961 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
    25021 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
    25081 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
    25141 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
    25201 cacgcccacg catgtaacgc aagccaataa atagagcgac aggttggtac atgaaatccg
    25261 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
    25321 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
    25381 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
    25441 gatacccatt acacctcggg tcataaccag caggatcaac cgcatgtaaa ttaaaaaaga
    25501 gcgtgttgtt ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
    25561 ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatatga ccacttttca
    25621 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
    25681 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
    25741 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
    25801 ttggggcgtg gtgcagtggc tggcacctga gcgcgattta agcaatgcct ccaatgccgc
    25861 ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca ccagcttgtg
    25921 gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc gtctggcgct
    25981 gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc ccacgccgtg
    26041 gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg catggtttgg
    26101 cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc tgatggcttc
    26161 tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc tgttgtcgct
    26221 ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat tcggtatgcg
    26281 ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca ccacccatcg
    26341 cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag cacgctggtc
    26401 gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt tcatctgtac
    26461 tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc tgttgttctc
    26521 cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc cagccggggc
    26581 gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt atcagagata
    26641 tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg cctgaacaat
    26701 atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg
    26761 cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg gtggtactca
    26821 ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag ttcaccgatc
    26881 agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt tcgcctcatc
    26941 aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg cagcgtggcg
    27001 tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt tttctccacg
    27061 gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta cgaacccaac
    27121 tggacagcgc cggttatcgc tatgttgacc aggtgatgga gcacggcgaa tacgccacgc
    27181 gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt cttgatttct
    27241 ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg
    27301 aagtagaagc gatcaatctg ctgcccgcac acgaatttcc gaccgataaa gcggcaattg
    27361 aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt
    27421 accagcaagt gagtaaaggc acattacctg ccgggatcga gtactggcag ccattgttct
    27481 tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg ctcgtgaata
    27541 ctggcgatct ggaaaccagt gccgaacgtt tccaggctga cacgctggcg cgttttgaga
    27601 atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg ctgcgggtgg
    27661 acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact gaacatttac
    27721 cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg gccgttcagg
    27781 cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc gacggtccgg
    27841 tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg ctcgcacgaa
    27901 ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc
    27961 tgatgattgg cgctgccgaa catggttttg tcgataaggt gcgtaatctg gcgctgatct
    28021 gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct cgccgcacca
    28081 tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag ccggtggtcc
    28141 atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct ggcggcatta
    28201 ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt ccggtgtcgt
    28261 cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac
    28321 ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg
    28381 cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac
    28441 acgatcgtga gcagtatcag ttgttctgcg acagcttccc gtttgaaacc acgccggatc
    28501 aggcgcaggc cattaatgcg gtacttaacg acatgtgtca gccgctggca atggatcgtc
    28561 tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg
    28621 cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt gcgcagcagc
    28681 attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa atgatctccc
    28741 gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg
    28801 atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa gatttaggcc
    28861 tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc attaaagcga
    28921 tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt acgctgaata
    28981 tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc cgtcgtctgg
    29041 cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg atcctgcgtg
    29101 aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac attcagaaag
    29161 ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt cacgggcaga
    29221 tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc
    29281 tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac accattatca
    29341 ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt cgcgtcggac
    29401 gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg atgactaccg
    29461 atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca ggttttgcgc
    29521 tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa gaacaaagcg
    29581 gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac gccgtcgatg
    29641 cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa acagaagtcg
    29701 agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac acgcgtctgt
    29761 cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag atcaaagtcg
    29821 agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg gatattgccc
    29881 gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat gagaaaggcg
    29941 ggatgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt ggtttgctgc
    30001 aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt attcaggatt
    30061 tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga
    30121 acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa
    30181 ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt
    30241 ttattacaat aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct
    30301 cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc
    30361 aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa atttcatgtg
    30421 gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc
    30481 gctctgttac aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta
    30541 acgatcccgt tgccaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt
    30601 gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata
    30661 ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac
    30721 aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag
    30781 ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg
    30841 attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc
    30901 attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc
    30961 tttagctagg tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct
    31021 gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat
    31081 gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca
    31141 gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg
    31201 gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc
    31261 cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg
    31321 cttatttata aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac
    31381 ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca
    31441 gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct ggcgttgact
    31501 gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc gcagcgatga
    31561 gggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg caagaaatta
    31621 cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga tattttttat
    31681 aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag caattatttt
    31741 tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt cattcacagt
    31801 caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg
    31861 ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat tgttctggca
    31921 acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc
    31981 cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta
    32041 tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga aaagcgtgga
    32101 gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca aagacacccc
    32161 ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag gagatatcgc
    32221 caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt ttttatgtca
    32281 acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg cagaattcct
    32341 taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact
    32401 gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat aagtcgcaaa
    32461 aaaagcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc ttgttatact
    32521 ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc cggatcgcgg
    32581 tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata accgagcatt
    32641 ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt
    32701 tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc ccccacaaca
    32761 gtggcgacat ctttaccgcg accaccacca aactgatgcc ctatcacgcc accagcaaca
    32821 gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg atgggtcact
    32881 gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa
    32941 acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac tgccgctacg
    33001 cccaacgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt cacgggaaca
    33061 cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc tatcagacag
    33121 aagatgaaaa gcgcgggaat gactggaaat acttaaggat tcggaggagt ctgaaggaat
    33181 gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc
    33241 ggataaggcg tttatgccac atcagccagt gtacgtcgat taatgcaact tcaaacgcgg
    33301 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
    33361 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
    33421 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg agaagttcga
    33481 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct taccgttcat
    33541 ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg
    33601 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
    33661 tggatcgcga gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttagtttc
    33721 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc ataccatcag
    33781 atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag cactggtgac
    33841 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
    33901 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
    33961 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgatcg cgttgtgcaa
    34021 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc
    34081 atttgcgccc aggttggcag agtatttcag gaacagattc agcatctcct ggtggaagcg
    34141 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacgcctg gcgtattgaa
    34201 atcgttagag gtgctaccca gcgccatgac cagggtgtca taggcgattt tacgttccgg
    34261 aaccaacagt tcacctttct cgtcgcgtag ttctgcaata gtgattgttt tcgcttcacg
    34321 atcaatatca atgacggaac ccaactggaa ctggaaacca tgattgcgcg catgggccag
    34381 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagtgg
    34441 tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttacgtcc
    34501 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
    34561 aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
    34621 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
    34681 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
    34741 ttgcttaaaa gataggcaac tttaccgggg gagatcaaaa aatagcgttt attatttgag
    34801 caaattcgga agagtaatat agggtgaagc gtatatcgag ggcgtttggt cgtcctgata
    34861 agatgcgcag catcgcatca ggcattgatg ctcgactgtc ggatgcggcg tggacgcctt
    34921 atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg
    34981 gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag atattcttga
    35041 atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg
    35101 tccgctggct gttaagtgct tcatcattac gcgacaaaat aaccagacaa cgatcgcgat
    35161 tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat
    35221 caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga
    35281 tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc caggccaacg ccgcaaatta
    35341 gcggacgctc gtcaacgttc agttgcaaca ttttgtccac ttctttaagc agatgctgca
    35401 tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact
    35461 gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga
    35521 taatcatcgc ttcctcacat aagccagggt tagtgacctg ctttctcttc ctgccagcgt
    35581 tcatgtaact gattcagacg ggtgctgatc gctttccagc gagccgagtc catcagttcc
    35641 tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt gatcggtgac
    35701 agattatcta acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa
    35761 cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatcgc ggcaccttcc
    35821 atcgataaat cgtcagagaa aatcacgccg tcaaaaccca gttcctgacg caaaacggtt
    35881 ttcagccagt agggagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc
    35941 gcaggcataa tggcgtcgag tttattttcg cggattaagg aactgaagac cgacatatct
    36001 ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc
    36061 gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcata
    36121 ccatcaataa accggctggc aattgccagg gctttttgtg gatcggcatg ataagaacgc
    36181 tcgccaatcg ccgcgctgat atgcccgaca tccagcactg gcgcaaagct gatatcaata
    36241 tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc
    36301 tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg
    36361 cgaaaacgct gcacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat
    36421 gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta
    36481 aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt
    36541 tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa
    36601 tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg tttgtcgcca
    36661 gcggtactca aaccaccctc ctttgagcat cagcagccag ggaaaccatc gcctgacctg
    36721 acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg
    36781 gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc
    36841 agcatactcc cagtcgatga gttttaaccc tgatgcgcta tgcactaaat ttccggcgtg
    36901 gacatccata tgcaatggac ttaagcgtaa aggtcgtggt tcccgcgctt tgcgcagacg
    36961 ttttaacatt cgcagccaac ccacagtccg ccgcgccgga tcgctttgct gccagtacag
    37021 ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gtagataata
    37081 cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcgc cgggcagata
    37141 gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag
    37201 ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg
    37261 ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc cactaagccc
    37321 actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg
    37381 cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct
    37441 gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag gcgctggagt
    37501 acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta cgggtgccta
    37561 aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagttgc tgggcggaaa
    37621 ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg
    37681 cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg ctacccgcag
    37741 tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat
    37801 aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc gtgctcaatt gggcctggct
    37861 gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt
    37921 gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg
    37981 cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc
    38041 acccctttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc
    38101 gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg
    38161 catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg
    38221 cctttcgtta taaagcgttg aggatgctga aggttgaata tcagacgtcg aaaggacggg
    38281 cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc
    38341 attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaaca ccagagacac
    38401 cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc
    38461 ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg
    38521 gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc
    38581 gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc
    38641 ggaatgagga tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga
    38701 aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt
    38761 ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg
    38821 ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc
    38881 aaccttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta
    38941 ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa
    39001 tatgaatgcg tatatttctc atttgcattt gcaagcaaaa ttattcgcac actaaatata
    39061 accgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa
    39121 tgctttcaac acaatttaac agggataatc aatatcaagc catcaccaaa ccgtcactac
    39181 ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg
    39241 aagaaacggt gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca
    39301 gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt
    39361 cggtcactat tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag
    39421 tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt
    39481 attcccgcgg attccagatc gataactata tggttgatgg tatccccacc tattttgaat
    39541 cgcgctggaa tctgggcgac gcactttctg atatggcact gtttgaacgc gtagaagtag
    39601 tgcgtggcgc gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg
    39661 ttcgaaaaca cgcgaccagt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct
    39721 ggaacaaaga acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc
    39781 gcgcgcgaat tgtcggcggc taccagaata acgactcatg gctggaccgc tacaatagtg
    39841 aaaagacctt cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag
    39901 ccggttacga atatcagcgc attgatgtta atagcccaac ctagggcggt ttaccgcgct
    39961 ggaatactga tggcagcagc aacagttacg atcgcgcacg cagtaccgca cctgactggg
    40021 cgtacaacga taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata
    40081 cctggcaagc gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt
    40141 atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg
    40201 gacctggctt tgatcatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg
    40261 cgctggattt gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctaatgt
    40321 ttggtggcag ttacagcaaa caaaacaatc gttacttcag ttcatgggcc aacatcttcc
    40381 cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaaacc gactggtcac
    40441 cacagagcct ggcgcaggac gataccacac atatgaaatc gttatatgct gccactcgtg
    40501 tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac tggcgggttg
    40561 atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg
    40621 acatcaatga caactggtcg acctacgcca gctatacctc tattttccag ccgcaaaatg
    40681 atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac gagctgggtc
    40741 tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc cgtattgagc
    40801 aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc gaaaccgcct
    40861 ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta
    40921 ccgacaactg gcagctgaca tttggcgcaa cgcgctatct tgcagaggat aacgaaggaa
    40981 acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc tatcggttgc
    41041 ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg
    41101 acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg ctggtggatc
    41161 tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc aataacctgt
    41221 tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca ccgcgtaatt
    41281 tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca gccatctggc
    41341 tgccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca tccatctcgg
    41401 ttttcaggtt atcggattta gtaccgaaaa tcgcctgaac accagaacca gcaaccacta
    41461 cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca tcagcaacgc
    41521 tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta ccaccaaatg
    41581 cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt
    41641 cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag atggtgtagt
    41701 aaacaatcgc ataaccgata ccgacgatcg ggaacagcca cagtttgctg ctgttaccag
    41761 acagaacgat gaagtcgatc agaccgtgcg agaacgacgt accgtcacgc attcccagaa
    41821 gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg
    41881 caacgaacat gaagaagaac tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg
    41941 ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca gagtgccaga
    42001 tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaagccacca gacagtttac
    42061 ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg
    42121 cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa
    42181 acggtaccag acaacgttcg atgaaaccgt aaatgccaaa cgcaactacc gggttctggt
    42241 aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa atgaaggaca
    42301 gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag cgtttaccgg
    42361 caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac atgtacgctg
    42421 caatcgcacc ggagataatc cctccgagta cgccagtatc cgccaagtgt ttagaggcga
    42481 tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt ttaaccatga
    42541 tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta aagccgaggg
    42601 cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca
    42661 taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa
    42721 tacctgcgat aggtagtacg gataccggca gcatcagcga tttaccgacc ttttgcaggt
    42781 tagcaaatgc attcttaaac ataattgaga gtgctcctga gtataggtgc tttttttacg
    42841 ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa gcgcccttta
    42901 tttattacac agagtaaaat aattcagtgc caatttgttt gacggctatc acgtttcaac
    42961 ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata tttcaaacgc
    43021 ggttattcac cgcccaattt tcggcggtga actttactcg ttttagccat taattacgag
    43081 ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg tgaaacagac
    43141 gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct ttcaacacag
    43201 ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct cgatgcggta
    43261 ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg atccaggggg acataacgcg
    43321 cagcatcgca cagttgctcc gcattacgga aggtcacaat gccggaaaag gagatgtaaa
    43381 atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc
    43441 caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggca cgggcgtcac
    43501 gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga tggatgaagg
    43561 actcttgctg acgtacttta gtttccggtg tgtaataata atccagcccg gtttcaccca
    43621 gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg tagggatcat
    43681 tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg cctaccagat
    43741 cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc
    43801 gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca taatccagac
    43861 catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag atgaggaacc
    43921 ggtagcacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga
    43981 agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca aacatccccc
    44041 agtatagcct gcaggcgcga gggagaaaga tggtttgcca gttcggcgac caggcccggc
    44101 acatcaacat tggtcaccag cgcagcgcca tgatggcgtt ttagcgcatc catcagcaac
    44161 gttgccaccc agtgtaaacg cgccggagct tgttcatgat taagggccgc cagcagcgaa
    44221 taccagtcgc ccgatggcac gctatatgcc aacgcctgac acaatgtttc acgagcctgc
    44281 cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat
    44341 gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaagt cacggcgtac
    44401 tgttctggag gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa
    44461 cgttcaggct cgcgggtagc caggaaaaac caggtttctg ctggtggctc ttcaagcgtt
    44521 ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac ccagacaact
    44581 ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg taccgcatca
    44641 atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg atgcgtgcca
    44701 gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg cggttgttgg
    44761 cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc
    44821 tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag tttttcgaaa
    44881 tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag tgggtcacgg
    44941 tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg gtatgaatac
    45001 ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag aaatcgaaag
    45061 actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg
    45121 gggtaacatc gagatagagc gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca
    45181 gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc tgagtggaga
    45241 gatcgtggcg atcgccaatc acccaggtgc cgttagccag cgcaggtttg atgaccgttt
    45301 ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg gtaatgactt
    45361 catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca agttgcgtac
    45421 cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc gtctcaacca
    45481 ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg atatacttac
    45541 tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt atgactggca
    45601 agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag atacggcgtt
    45661 tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat cgcacccggc
    45721 ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa
    45781 agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc
    45841 ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc actggcaacg
    45901 gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc tttataaggc
    45961 agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt cttgtgagct
    46021 cgcttgagta acgcgacatc ggtggtattg gcggtataca tcaaggtgtc tggccagaac
    46081 caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt ggcgtactta
    46141 tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag gtagtcgctc
    46201 agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct ttccaacaat
    46261 ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc tttaaagtga
    46321 gaaagatccg gttcgatacg cagcagccat tgaaaaaccc gtgggcgatt gatgatctta
    46381 tcagcataaa gctgttcacc gagcgccaga cgtccggtcc ctggcttcag ggtaaatatc
    46441 gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca gacgcccaca
    46501 ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac ttttttcatg
    46561 actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct gacgaaaagg
    46621 tgacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa ataaccatct
    46681 catctgcctg caacgcctcc tccagcgagg cttgcacttc gacaagctga taagaggatt
    46741 gtgccagtaa acggatacag aattgtcgca taatgccgtt aacacctgcc tgatccagtc
    46801 gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag cattccgtaa
    46861 cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca agatgagagc
    46921 gaatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga ggattgcgcc
    46981 ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg
    47041 caggataagc cgtaacggag agaatccgcg ttgctggtcc gctgttcaat gtgctgtacc
    47101 ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc tgttctgctg
    47161 ccagcgtttt catctcttgt tcaagctgag gccagaagtc acaggaaatc atcaaccgct
    47221 gacaagcatc ctgtagtcgc tggatatgcg ccgataacaa actgacttta ccgtcgataa
    47281 ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt actgccagcg
    47341 attcctgctt ataaccgtta attaagaaca taccggctcc ttatcagaga attgttagtg
    47401 tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc
    47461 ggacctttta tatgggtgga aaatgacaac ttagatcttt ttaaagatca aagaaccatt
    47521 agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga cctgacgtgc
    47581 ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt tgatggttgg
    47641 cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg cacccgccgc
    47701 acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc ttgcagcttc
    47761 accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg tagaagtacc
    47821 gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca gagcatttgc
    47881 cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat aagcatcgct
    47941 gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc
    48001 gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat cacgctcttt
    48061 atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg cacgtgccgc
    48121 gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg caaccatcac
    48181 gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca cgccggaagt
    48241 acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag tcagatgacc
    48301 tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac gtggaccacc
    48361 gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc cggagccaat
    48421 tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat cctgcatggc
    48481 ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct gttctttgcg
    48541 cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg ttgcataggc
    48601 gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca gagctttcca
    48661 ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca caactacacg
    48721 acgcttagac acgtttgtcc tccaaggagg gaaaaatgat tctagtggga caaaaagata
    48781 aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg gccgttgatg
    48841 taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg aatctcagta
    48901 tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc cgcgcccagg
    48961 tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag ctgttcgccg
    49021 ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct atcaaaactc
    49081 gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact aaatcccggc
    49141 aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg caaatcattt
    49201 tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat
    49261 cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt gatgtaagct
    49321 gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga
    49381 cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc acgtgtcatg
    49441 tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga cgcaacttcg
    49501 cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta gttggcctga
    49561 ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc atgacgcttt
    49621 ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag gttagtttcg
    49681 ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg agtgataccg
    49741 gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc cagaacagat
    49801 tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttggc acctaaataa
    49861 tcactgatcg cctgagagcc attttcactg gtcgcagtgc caataacttt cgcgccacgg
    49921 gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt taccagtgcg
    49981 atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc tgccatcgct
    50041 gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta
    50101 agcaccttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc catgtactca
    50161 acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc acggatggca
    50221 tcaccattgg tttcgcattt cacatcaacg ttattcacaa cagaaactgt tggtgcgtta
    50281 aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat cagcgcacag
    50341 tgagacggta cgctcactgg taacggcagc gcgcgttttg cgccagcagc tttacaggca
    50401 gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc cggagagtta
    50461 aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt cgcaatagac
    50521 gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttccggtac ggcttcttgc
    50581 atgaacttac cgcgcatctc aaccaaacgc accgcatcag cgaaatcaat cacgccagcg
    50641 caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg tgctttaccg
    50701 ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc tggctgggtt
    50761 tgccaggttt tattcagttc ttcagctggc ccctgctggg tcagcgccca caggtcgtag
    50821 cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct cgccgccata
    50881 tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc
    50941 atgttttaat ccttatccta gaaacgaacc agcgcggagc cccaagtgaa tccaccgcca
    51001 aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc
    51061 agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt caccacgaca
    51121 ttgtccatcg acataccgag ttttttcgcc gttgcactga taatacgcag gttagcctga
    51181 tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag cgtctcatca
    51241 acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc catcgtcaga
    51301 tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa ttcaccataa
    51361 ctaccgtcgg catgcagatg ggtggaaatg attcccggct cttcagaggc agccagcacc
    51421 gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg atcgcaggtg
    51481 cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga tttcacgtat
    51541 tgatcggcta cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc
    51601 gggcaacctt taatgcccaa catgctttga atctgacaag ctgcgctcgg gaaagcgtgc
    51661 gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat gcccgccatc
    51721 tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca
    51781 atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt gtccaccatt
    51841 ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt accaataatc
    51901 ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc aaactgcgag
    51961 ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca gcagctcaaa
    52021 accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac
    52081 cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg caccatgact
    52141 ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt cggggttgag
    52201 gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca gtagccacca
    52261 cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc tgacaacacc
    52321 ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca catctgtctt
    52381 gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag ggattgtttt
    52441 aagcaccgct gaggcatccc gaatactgtc aagacccttt acttcttctt caccaatatt
    52501 gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa ctgagcccat
    52561 aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta aatcgaggac
    52621 caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac gctcaatccc
    52681 ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat taccggcact
    52741 gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca ttgaactccc
    52801 acgactggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg actgcgcagg
    52861 aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc
    52921 gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc
    52981 tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg ccagggttag
    53041 acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg gggaaaatcc
    53101 tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg gccgcggtag
    53161 taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt gtctacagac
    53221 aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt
    53281 ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat ccccgatcca
    53341 acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg gtttggcttt
    53401 tgcacttctt caggcagttc accaaagacc atatccgctt cggacacttc acagtgttca
    53461 gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac cattgcaagc
    53521 agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg cagtgcttca
    53581 gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg atgagtaaac
    53641 ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact
    53701 gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact gtccacactg
    53761 actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg gtaatcaagg
    53821 cgtttttgag ccgtacgaac cggatcgaga gtcaggggga attttacctt ttgcataggg
    53881 cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt
    53941 ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt gctgataacg
    54001 aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat acgctatatc
    54061 tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg cgtcgcgctc
    54121 ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc tgaggtcgac gaaaccccac
    54181 gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc
    54241 tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt gttcttgatg
    54301 gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca
    54361 gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg aatgggcatc
    54421 tacaaacaga agtggagcct tttgacgtcc atttccgtca cctaagcgag gcggagattg
    54481 ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag agtgaaggat
    54541 ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg gttggtttac
    54601 cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg atgggatagt
    54661 tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc
    54721 gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta ttagcgcgcg
    54781 ttacgcagtt tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc
    54841 acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt
    54901 aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca
    54961 aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt acgtccggtt
    55021 accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt
    55081 tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg cagaatattt
    55141 ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg caccagcgcc
    55201 aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg caacgccgag
    55261 cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg aaccagttca
    55321 aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa gcttaaacca
    55381 ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg atcatcttca
    55441 tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac cgcctcttct
    55501 tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc gagtttatat
    55561 tcaggcttaa tacgtttttt gttcacccgc acttcacctt tacgcaaaat acggtaaatc
    55621 atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg ttgccccgct
    55681 tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat ggtgggcgat
    55741 tctaattagc caacaggatt cgcgccactc atttttctat gcttatattt agttttgcac
    55801 cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca ccagcaagaa
    55861 gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct ataacaaggc
    55921 ttgcagtgga ataatgaggc cgtttccgtg tccatccttg ttaaaacaag aaattttacg
    55981 gaataaccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac gtattgatct
    56041 ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa tatttctgga
    56101 gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga aaaacgggca
    56161 accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac gcgactagac
    56221 acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg
    56281 taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc agcaggaaga
    56341 gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctagatatcg aaagtccagg
    56401 gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg aaccgagtct
    56461 ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac taaaagaaat
    56521 tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca ttaaagatgt
    56581 gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg gcaacaaagg
    56641 cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga tgccaaataa
    56701 cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga
    56761 agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca ccgctggcgt
    56821 aggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac actgggaagc
    56881 catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg attcatcagg agagcaacgt
    56941 aatcgttcgc gccttccgcg attatttacg tcaggatatc ggcgaaatcc ttatcgataa
    57001 cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc cggatttcag
    57061 cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc agatcgagtc
    57121 gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca tccggtggtt ccattgttat
    57181 cgacagcacc gaagcgttaa cagccatcga catcaactcc gcacgcgcga cccgcggcgg
    57241 cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc gccgatgaga ttgctcgtca
    57301 gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca tgacgccagt
    57361 acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg
    57421 tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag
    57481 cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt
    57541 gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg atcgaagaag aagcgctgaa
    57601 agagaacacc caggaagttc acgccattgt tcctgtgcca atcgcttctt atctgctgaa
    57661 tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag gacggtgttc gctgcgtgat
    57721 tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga
    57781 agaaacgccg accttaagct acatgctgcc gaagctgcat gaagaagcga tggcgctgcc
    57841 gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg caacctttgc
    57901 catgccggat gtgccgccag cgccaacccc agctgaacct gccgcgactg tcgtagcccc
    57961 agcaccgaaa gctgcaacgg caacaccagc agctcctgca caacctgggc tgttaagccg
    58021 cttcttcggc gcactgaaag cgctgttcag cggtggtgaa gaagccaaac cgaccgagca
    58081 accaacaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca
    58141 gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta ctgaaggcag
    58201 cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaaa ctgccgagac
    58261 gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca
    58321 agcgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg cgcaacaaga
    58381 agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg aagaacgtgt
    58441 acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc gttacgagca
    58501 aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc
    58561 aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc tgccagtcgt
    58621 agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg acaacggtgg
    58681 catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg
    58741 tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc
    58801 gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac gtccgcaaga
    58861 tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga tggtgactga
    58921 ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg ccagttgttg aagaaatggc
    58981 tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg ttgaaacgac
    59041 gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta ccgagtctga
    59101 tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg gtggttgaac cgcaggaaga
    59161 gacggcagat attgaagaag ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt
    59221 tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc
    59281 cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa tgacgcgcgc
    59341 tccggcaccg gaatatgttc cggaggcacc gcgtcacagt gactggcagc gccctacttt
    59401 tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg cctctgccgc
    59461 tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag ccctggtaac
    59521 tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca aatggcatcc
    59581 ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat cccagagcgg
    59641 gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt gttgagctgg
    59701 aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa tgccgtttgc
    59761 tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc gctcatctgc
    59821 tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag tttgttcagc
    59881 tgcgtgccta attccgtgcg aacggtaagg acattattca gcgagttttt cagtccacgg
    59941 ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc cgcgaccggc
    60001 gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc agaagcgcta
    60061 ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc gcccgtatgt
    60121 cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt tccgccgaca
    60181 taatcaccgt tagcctcact aaacggcgca gtctctgttt tataaccggc aaaaatgtag
    60241 cgcccattac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag cccctgaata
    60301 tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt
    60361 ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc ttccagtgac
    60421 actttttgag tggcgaaagt acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg
    60481 gagagaacaa cagcttgtga tgcagcaatg ggatcgtctg aagggttaac gactcgctta
    60541 cccgtcgaca tctgctcgcc gtacttcatc cattctgcct gagaattggt gatgccgcgc
    60601 atgttttgct ggtacatcat ctgggtactg aaacgcattt tatctcccct tagcgaatgt
    60661 taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat
    60721 actgctgaaa acgttgcaga tttccgtatt cctcatcgag attgacaccg gaaatcgact
    60781 gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggtgctactg gttttcaacg
    60841 tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta aaggatttcg
    60901 caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg ttgcggttgt
    60961 cgctatcacc cgcatcttct tcgctcgcca tcgcgatttt cgcttcgtcg gtgattaata
    61021 catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg ttaacggcag
    61081 gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg gcatctggtg
    61141 tcgccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta ttatcgaacg
    61201 agattttgta atctgtcgcc agtaccacgg aggcatcagt taccgtggca ccgatcgcaa
    61261 cgtcaccttt gtttttcgtg ttttgctgaa ccgcgggatt accgatagta aagaaatctt
    61321 caccagcatc gccgttagca tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa
    61381 atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagatcctga gaacggaatg
    61441 tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca atattgcctg
    61501 ccgtcccatc aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa ggaactgccg
    61561 ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggcatcg tgatgttata
    61621 agtaccgcca tcctgaacgc tgacttctac gccaacaatc tggtttaatt cgctcaccag
    61681 ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg
    61741 cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct
    61801 ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt
    61861 aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgccg ccgggtcttc
    61921 cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga
    61981 agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcggtcag
    62041 gccgctactt tgcgtctgca cagcacgtaa ctggttggta ataaacgtat catactcacg
    62101 ctgcacacca gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt
    62161 ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt
    62221 attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat
    62281 caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc
    62341 cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc
    62401 tggtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga
    62461 tcggtagcat agcccgcgtc ctgtaggacc tgcgccccct gttccgcact cgcggcggtc
    62521 gtcacggcgg cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc
    62581 agatacgagc tgtagacccg aaacttcgct tttactttct tcgcttcgcc gttttcatat
    62641 tcagtcgtgg tgatttcagt gacttgccct ttccagttgc cagaggcttt gacaccaaac
    62701 aggttatagc ttggctcgcc gttttcgcgg cggatttgcc gttgccccca accagattca
    62761 agcgcagcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg
    62821 gcgggcagcg agagttgcgc gaggaatgct ctactgtcac ccggcagcga atcatcgtag
    62881 ttacgtggta cggccttttg caccagctgc gaaagcgtct ggttttgata acgcaccacg
    62941 gtttcgagcg ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct
    63001 ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt
    63061 tgggcaattt gctggtcata catactggta tacagacgag tgtgctcgct gctgaacagg
    63121 ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc
    63181 acctgacggg ccaccggacg gatatttgcc gccggatctt cgctcgcttt cgcctttagt
    63241 tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc
    63301 agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc aaaatagaca
    63361 tcagatccat cggcgtagcg cccagcgcat tgagcgcacg caccacgtta ttgaggctgg
    63421 cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg
    63481 gagtaacaac agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac
    63541 gattaactgt tactgagaga tttccctgcg ctaccgcgca gctgtcgagg gtcacttctc
    63601 gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga
    63661 cattaacctg catattctgg atatcggcaa ggaaacggac ctgggaactg ttgccacttg
    63721 gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc
    63781 ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat
    63841 taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg
    63901 tgatccgtcc accgttcagt tggttaacct gaacactgct accgcccgcg gaggctcctg
    63961 cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa
    64021 gcggtgtcat caacaacgta cctccacgca agcttttggc attacccatg gaagaaacca
    64081 ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgacactgtc accattacca
    64141 cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga
    64201 gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccagtgccat
    64261 ccagccccac taccaggcca tagccaatca gtgagttttg ccttaccccc tgaacactgg
    64321 tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag
    64381 agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg
    64441 caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt caatacgcgc
    64501 atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac
    64561 gcccgagaag cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac
    64621 atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt
    64681 attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc
    64741 ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt
    64801 agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg
    64861 caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg
    64921 atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg
    64981 accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc
    65041 gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt
    65101 ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttatgcctt
    65161 agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt
    65221 cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg
    65281 tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg
    65341 gtgcaccgga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agtccggtat
    65401 cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt
    65461 gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg
    65521 tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc tgatccacct
    65581 ggaaagagcc gtcacgggta taggctgatg agccatctgg caacattacc tggaaaaagc
    65641 cctgcccttt aatcgcgaca tctttgctgt tattggtctg cgacagattg ccctggctgt
    65701 gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg
    65761 tggtttgttc ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa
    65821 acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga
    65881 cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca
    65941 tataatgtcc tgtagtggat tgctgacaag cagagcagaa gcacgcgaca gtgtgaattt
    66001 cccctcaccc taaccctctc cccaaagggg cgagggaata gtccgcgcgc ttttattgca
    66061 atgtcatcaa tcgttcaatt aactcatcga cagcagttgg ttggcacggc ctgcgttatc
    66121 atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc
    66181 gctcattgcc gcaacggcat tgacgttact gccttccaga acccccgaca tcacacgcaa
    66241 ggttggatct gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc ttaaacgaaa
    66301 aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt tcagacgccc
    66361 tactggcgca actgtatttg ccggatcgcc cggattgagc gccgagattg tgccatcggc
    66421 agcaatcgtg atttccgccc cttccggcac agcaattggc cccgcctcgc ctatcaccgg
    66481 atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat tacgcgtata
    66541 cccttcgctg ccgtcagcgg tctgcacggc cagccatcct gctgcaacgc aacgtccagc
    66601 gggcgcgagg tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt
    66661 accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac
    66721 tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga
    66781 ttcagcgtct ggctggctgc tcccatcgca gtataaattg cgtgatccat tgagctatcc
    66841 cgtcagcgat tagcgtaagt taacccgcgt gttgaggatc tggtcctggg ttttgatggt
    66901 ctgggcgtta gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag
    66961 atcgacgttg gacgcttcca gcgcaccgtt ggtcagggtg ccaaagtttc ccgtcccggc
    67021 tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga
    67081 tgccagacct tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgggtttg
    67141 ttcgttggaa tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc
    67201 gcccggtttg tagccattct gggtggt
//
LOCUS       DAJTYI010000005        66505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYI010000005 DAJTYI010000000
VERSION     DAJTYI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..2385)
                     /gene="glgP"
                     /locus_tag="OO564_000344"
     CDS             complement(<1..2385)
                     /gene="glgP"
                     /locus_tag="OO564_000344"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6651604.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFS"
     gene            complement(2404..3837)
                     /gene="glgA"
                     /locus_tag="OO564_000345"
     CDS             complement(2404..3837)
                     /gene="glgA"
                     /locus_tag="OO564_000345"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6651605.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3837..5132)
                     /gene="glgC"
                     /locus_tag="OO564_000346"
     CDS             complement(3837..5132)
                     /gene="glgC"
                     /locus_tag="OO564_000346"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6651606.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5150..7123)
                     /gene="glgX"
                     /locus_tag="OO564_000347"
     CDS             complement(5150..7123)
                     /gene="glgX"
                     /locus_tag="OO564_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6651607.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7120..9306)
                     /gene="glgB"
                     /locus_tag="OO564_000348"
     CDS             complement(7120..9306)
                     /gene="glgB"
                     /locus_tag="OO564_000348"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6651608.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9579..10682)
                     /gene="asd"
                     /locus_tag="OO564_000349"
     CDS             complement(9579..10682)
                     /gene="asd"
                     /locus_tag="OO564_000349"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6651609.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10874..11467
                     /gene="yhgN"
                     /locus_tag="OO564_000350"
     CDS             10874..11467
                     /gene="yhgN"
                     /locus_tag="OO564_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6651610.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11524..12864)
                     /gene="gntU"
                     /locus_tag="OO564_000351"
     CDS             complement(11524..12864)
                     /gene="gntU"
                     /locus_tag="OO564_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6651611.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12868..13395)
                     /gene="gntK"
                     /locus_tag="OO564_000352"
     CDS             complement(12868..13395)
                     /gene="gntK"
                     /locus_tag="OO564_000352"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6651612.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13534..14529)
                     /gene="gntR"
                     /locus_tag="OO564_000353"
     CDS             complement(13534..14529)
                     /gene="gntR"
                     /locus_tag="OO564_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6651613.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14753..15448)
                     /gene="yhhW"
                     /locus_tag="OO564_000354"
     CDS             complement(14753..15448)
                     /gene="yhhW"
                     /locus_tag="OO564_000354"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6651614.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15571..16608)
                     /locus_tag="OO564_000355"
     CDS             complement(15571..16608)
                     /locus_tag="OO564_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6651615.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16941..17429
                     /gene="yhhY"
                     /locus_tag="OO564_000356"
     CDS             16941..17429
                     /gene="yhhY"
                     /locus_tag="OO564_000356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6651616.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17488..19210)
                     /gene="ggt"
                     /locus_tag="OO564_000357"
                     /pseudo
     CDS             complement(17488..19210)
                     /gene="ggt"
                     /locus_tag="OO564_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19330..19770
                     /locus_tag="OO564_000358"
     CDS             19330..19770
                     /locus_tag="OO564_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6651617.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19757..20500)
                     /gene="ugpQ"
                     /locus_tag="OO564_000359"
     CDS             complement(19757..20500)
                     /gene="ugpQ"
                     /locus_tag="OO564_000359"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6651618.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20497..21487)
                     /locus_tag="OO564_000360"
                     /pseudo
     CDS             complement(20497..21487)
                     /locus_tag="OO564_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21489..22334)
                     /gene="ugpE"
                     /locus_tag="OO564_000361"
     CDS             complement(21489..22334)
                     /gene="ugpE"
                     /locus_tag="OO564_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6651619.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22331..23218)
                     /gene="ugpA"
                     /locus_tag="OO564_000362"
     CDS             complement(22331..23218)
                     /gene="ugpA"
                     /locus_tag="OO564_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6651620.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23316..24632)
                     /gene="ugpB"
                     /locus_tag="OO564_000363"
     CDS             complement(23316..24632)
                     /gene="ugpB"
                     /locus_tag="OO564_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6651621.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25029..25742)
                     /gene="livF"
                     /locus_tag="OO564_000364"
     CDS             complement(25029..25742)
                     /gene="livF"
                     /locus_tag="OO564_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6651622.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25744..26511)
                     /gene="livG"
                     /locus_tag="OO564_000365"
     CDS             complement(25744..26511)
                     /gene="livG"
                     /locus_tag="OO564_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6651623.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26508..27785)
                     /gene="livM"
                     /locus_tag="OO564_000366"
     CDS             complement(26508..27785)
                     /gene="livM"
                     /locus_tag="OO564_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6651624.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27782..28708)
                     /gene="livH"
                     /locus_tag="OO564_000367"
     CDS             complement(27782..28708)
                     /gene="livH"
                     /locus_tag="OO564_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6651625.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28756..29865)
                     /gene="livK"
                     /locus_tag="OO564_000368"
     CDS             complement(28756..29865)
                     /gene="livK"
                     /locus_tag="OO564_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6651626.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30289..30672
                     /gene="panM"
                     /locus_tag="OO564_000369"
     CDS             30289..30672
                     /gene="panM"
                     /locus_tag="OO564_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6651627.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30871..31974)
                     /gene="livJ"
                     /locus_tag="OO564_000370"
     CDS             complement(30871..31974)
                     /gene="livJ"
                     /locus_tag="OO564_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6651628.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32245..33099)
                     /gene="rpoH"
                     /locus_tag="OO564_000371"
     CDS             complement(32245..33099)
                     /gene="rpoH"
                     /locus_tag="OO564_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6651629.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33344..34402)
                     /gene="ftsX"
                     /locus_tag="OO564_000372"
     CDS             complement(33344..34402)
                     /gene="ftsX"
                     /locus_tag="OO564_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6651630.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34395..35063)
                     /gene="ftsE"
                     /locus_tag="OO564_000373"
     CDS             complement(34395..35063)
                     /gene="ftsE"
                     /locus_tag="OO564_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6651631.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35066..36562)
                     /gene="ftsY"
                     /locus_tag="OO564_000374"
     CDS             complement(35066..36562)
                     /gene="ftsY"
                     /locus_tag="OO564_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6651632.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36712..37308
                     /gene="rsmD"
                     /locus_tag="OO564_000375"
     CDS             36712..37308
                     /gene="rsmD"
                     /locus_tag="OO564_000375"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6651633.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37298..37567
                     /locus_tag="OO564_000376"
     CDS             37298..37567
                     /locus_tag="OO564_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6651634.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37570..37929)
                     /locus_tag="OO564_000377"
     CDS             complement(37570..37929)
                     /locus_tag="OO564_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6651635.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38070..38696
                     /locus_tag="OO564_000378"
     CDS             38070..38696
                     /locus_tag="OO564_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6651636.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38770..40968
                     /gene="zntA"
                     /locus_tag="OO564_000379"
     CDS             38770..40968
                     /gene="zntA"
                     /locus_tag="OO564_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6651637.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41070..41315)
                     /gene="tusA"
                     /locus_tag="OO564_000380"
     CDS             complement(41070..41315)
                     /gene="tusA"
                     /locus_tag="OO564_000380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6651638.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41536..42201
                     /locus_tag="OO564_000381"
     CDS             41536..42201
                     /locus_tag="OO564_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6651639.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42274..42831
                     /gene="dcrB"
                     /locus_tag="OO564_000382"
     CDS             42274..42831
                     /gene="dcrB"
                     /locus_tag="OO564_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6651640.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42835..44052)
                     /locus_tag="OO564_000383"
                     /pseudo
     CDS             complement(42835..44052)
                     /locus_tag="OO564_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44184..45233
                     /locus_tag="OO564_000384"
     CDS             44184..45233
                     /locus_tag="OO564_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6651641.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45288..45875
                     /gene="acpT"
                     /locus_tag="OO564_000385"
     CDS             45288..45875
                     /gene="acpT"
                     /locus_tag="OO564_000385"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6651642.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45986..47560
                     /gene="nikA"
                     /locus_tag="OO564_000386"
     CDS             45986..47560
                     /gene="nikA"
                     /locus_tag="OO564_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6651643.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47560..48504
                     /gene="nikB"
                     /locus_tag="OO564_000387"
     CDS             47560..48504
                     /gene="nikB"
                     /locus_tag="OO564_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6651644.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48501..49334
                     /gene="nikC"
                     /locus_tag="OO564_000388"
     CDS             48501..49334
                     /gene="nikC"
                     /locus_tag="OO564_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6651645.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49334..50098
                     /gene="nikD"
                     /locus_tag="OO564_000389"
     CDS             49334..50098
                     /gene="nikD"
                     /locus_tag="OO564_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6651646.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50095..50901
                     /gene="nikE"
                     /locus_tag="OO564_000390"
     CDS             50095..50901
                     /gene="nikE"
                     /locus_tag="OO564_000390"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6651647.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50907..51308
                     /gene="nikR"
                     /locus_tag="OO564_000391"
     CDS             50907..51308
                     /gene="nikR"
                     /locus_tag="OO564_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6651648.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51428..51787)
                     /locus_tag="OO564_000392"
     CDS             complement(51428..51787)
                     /locus_tag="OO564_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6651649.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51784..52059)
                     /locus_tag="OO564_000393"
     CDS             complement(51784..52059)
                     /locus_tag="OO564_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6651650.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52118..53242)
                     /locus_tag="OO564_000394"
     CDS             complement(52118..53242)
                     /locus_tag="OO564_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6651651.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53242..55977)
                     /gene="rbbA"
                     /locus_tag="OO564_000395"
     CDS             complement(53242..55977)
                     /gene="rbbA"
                     /locus_tag="OO564_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6651652.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55974..57041)
                     /locus_tag="OO564_000396"
     CDS             complement(55974..57041)
                     /locus_tag="OO564_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6651653.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57407..59029)
                     /locus_tag="OO564_000397"
     CDS             complement(57407..59029)
                     /locus_tag="OO564_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6651654.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59291..60880)
                     /locus_tag="OO564_000398"
     CDS             complement(59291..60880)
                     /locus_tag="OO564_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6651655.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61264..62295
                     /locus_tag="OO564_000399"
                     /pseudo
     CDS             61264..62295
                     /locus_tag="OO564_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62829..63593)
                     /locus_tag="OO564_000400"
     CDS             complement(62829..63593)
                     /locus_tag="OO564_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6651656.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63840..64601
                     /locus_tag="OO564_000401"
     CDS             63840..64601
                     /locus_tag="OO564_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6651657.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64708..65208)
                     /locus_tag="OO564_000402"
     CDS             complement(64708..65208)
                     /locus_tag="OO564_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651658.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65359..>66505)
                     /locus_tag="OO564_000403"
     CDS             complement(65359..>66505)
                     /locus_tag="OO564_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6651659.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16151 a        17851 c        16324 g        16179 t
ORIGIN      
        1 agagaagtag cccatattgg caatgttcag catcgctttt gcggtccact cttcctgaag
       61 ctcgtagagt tcgtcgactt tatcctgaca atcgacatag ctgcgataat ccgccagtac
      121 ctggtagtga tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc gacccggatc
      181 ttccggactg aatacaccgc tgccgatttg cgtcagcacc tgatgcagct cctcatcttt
      241 ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt tcttccactt cttccgctgt
      301 gttaccaaaa ataaagatat tgtcagcacc gacatgatcc agcatctcga cattcgcacc
      361 gtccaacgta ccgatagtca gcgcaccgtt aagcgcaaac ttcatgttac tggtgccgga
      421 agcttccgtc cctgccagcg aaatctgttc agacagatct gccgccggaa tgatcaactg
      481 cgccaggcta acgctgtagt tcgggatgaa cacgactttc agcttatcgc caatctgcgg
      541 atcgttgttg atcactttcg ctacgtcatt gatcaaatga ataatgtgct tcgccatgta
      601 ataggccgaa gccgccttac cgccaaaaat attcacgcgc ggtacccact tcgcatccgg
      661 gtcggccttg atgcggttat agcgggtaat cacatgcaac acattcatca attgacgttt
      721 gtattcgtga atacgtttga tttgtacatc gaacaacgcc tttggattca ccaccacatt
      781 cagctgctgg gcgatatact ctgccagacg ctttttgttc tccagcttcg cctgatgcac
      841 agcgtgatta accattggga aatcacagtg ttgttgcagc tcattaagca ggctaaggtc
      901 ggtgcgccag ttacggccca ggtgttcgtc cagcacggct gaaagcgatg ggttcgctac
      961 cgccagccag cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac ccgggaagat
     1021 tttcgcaaag tcggcaaaca acgattgcac catcagatta gagtgcagtt ccgatacacc
     1081 gttaactttg tggctcacaa caaccgccag ccaggccata cgcacacgac gaccgttgga
     1141 ttcatcaatg atcgacgccc gtcccagcag atcggtatcg ttcggatact gttcctgcaa
     1201 ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg gcagaatttt
     1261 acccagcata tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt ggttagtgta
     1321 ggagaagacc tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt gctcatcgat
     1381 cagcagacgc atcatctcag gaatcgacag taccggatgg gtatcattga gatgaatcgc
     1441 gattttatcc gccaggttat cgtaggtttt atgtaactga taatggcggc ttaaaatgtc
     1501 ctgaatggtc gaggaaacca ggaagtattc ctgacgcagg cgcagctcac gcccggagta
     1561 ggtggagtca tccggataca gtacgcgaga tacgttctcg gaatggtttt tatcttccac
     1621 tgcagcgaag tagtcaccct ggttgaattt accgaggtta atttcgctac tggcttgcgc
     1681 actccacaaa cgcagcgtgt tggtcgcgtc ggtgtcgtaa ccagggatta tctgatcgta
     1741 agcgactccc agaatctctt cggtttcaat ccagcgcgtt tttttacctt cctgctgaat
     1801 gcgaccacca aaacggactt tatagcgcgt gttgtggcgt ttgaattccc acgggttacc
     1861 gtattccagc cagtagtctg gcgactcttt ctggctaccg ttaacgatgt tctgcttgaa
     1921 cataccgtag tcatagcgaa tgccgtaacc gcgccccggc aaccctaacg tcgccagaga
     1981 atcaaggaag caagccgcca gacgtcccag gccaccgtta ccgaggcctg ggtcattttc
     2041 ttcatcaatc agctcttcga gatttaaccc catcgcttcc agtgcgccct gtacatcttc
     2101 gtaaattcct agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa actccatcga
     2161 caggtagtaa acctgacgag tttcttgcga caactgggca cggtttgaac gtaaccagcg
     2221 ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc agccattcat gtttattggc
     2281 gacgaccggg tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt gcttaagagc
     2341 ttctacgcta agcgtgggcg atgaatatgt aaacggagca ttcatatagg cgtttcctga
     2401 aaactatttc aagcgatagt aaagctcacg gtacgacttc gccgcgacct gccagctaaa
     2461 atccattgcc atagcctgac gttgcacaaa ccgccacagt gaaggacggg accacagtac
     2521 aaaagcacgc cgaatagccc gtaacagcga ccaggcatta ctatcttcaa agacaaaccc
     2581 actggcgacg ccatctgcaa ggttctcaag agaacagtca gaaaccgtat cagcaagccc
     2641 accggtgcgc cgcactaacg gcagcgtacc gtacttcaat ccataaagtt gcgttaagcc
     2701 acacggttca aaacggctgg gcaccagaat gacgtccgcg ccgcccataa tgcgatgcga
     2761 aaatgcttcg tgatagccaa tctgaacgcc cacctgaccg gggtattccg ctgccgccgc
     2821 aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt agcgccagct gcccgccctg
     2881 ctccagaagc cccggtaagg cttccagcac caggtcgaga cctttctggc tggtcagacg
     2941 gctcaccact gcaaaaagcg gcactttatc gtcaacctta agccccattg cgatttgtaa
     3001 ctggctctta ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg aggccaacag
     3061 taagtccgtc tctggactcc agattttctc gtccacgccg ttcagtacgc ctgaaagacg
     3121 cccttcacgg tgacgctgtt gcaacagacc ttccataccg taggcaaact gcggttcggt
     3181 gatctcgcga gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt acagaccggc
     3241 cttcaggaaa gagatttgtc cgttgaattc cagcccatga atattaaaga atgaccatgg
     3301 caattggatg tcattcatgt gatgtgcata aaacatgcct tgataggcca ggttgtgcac
     3361 agtaaacacc gacttcgcag gacgcccgcg cgccgccaga tacgcaggcg caagacctgc
     3421 atgccagtcg tgcgcatgca ccacatcagg acgccagaat gggtcaagcc cgctggccat
     3481 ttctgcccca acccacccca gcagcgcaaa acgcaatacg ttgtcggtat aggcaaataa
     3541 gttggtatcg tgatacgggc ttcccggacg atcatagaga tgcggcgcgt caatcaggta
     3601 tatgccaacc ccgttgtaat gaccgaacaa cagcgtgata tgcccggcga aagtatcacg
     3661 acgggatact acctgcgcat cggtcacgcc acggcgaata tcgggaaatg caggcaacag
     3721 tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa taacatcagc
     3781 cagaccgccg gttttaagca gcgggaacat ctctgaacat acatgtaaaa cctgcattat
     3841 cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg ttaccagcac gatgccttct
     3901 tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt caccaatcac catgccttcc
     3961 gggataacac aagcacgatc gatgacgcag cggcgcagac ggcacgagcg acctacccat
     4021 acttccggta acaatacggc ggaatcaatg ttgcagaatg aattcacgcg aacgcgcgag
     4081 aacagaacgg actgcaccac caccgaaccg gagatcacac aaccgccgga taccagtgag
     4141 ttaagggtca tcccgtggct accggagcga tcctgcacga atttcgctgg cggtaatgat
     4201 tcattgtagg tgcgaattgg ccaattgcga tcgtacatat ccagttccgg caccacagag
     4261 gccagatcga gattcgcttt ccagtaagct tccagcgtac ccacatcgcg ccagtacggc
     4321 tcggcatccg ggtcggattg tacgcaagag agcgggaacg ggtgcgcata ggccagaccg
     4381 gcttcggtga tcttgggaat caaatctttg ccaaagtcgt ggctggagtt ctcatcacga
     4441 tcgtcttctt ccagcagttc atacagatag tcggcgtcaa agacgtagat acccatactc
     4501 gccagagatt tgctcggatc gttcggcatt gacggtgggt tagcaggttt ttcaacgaat
     4561 tcgataattt tatcgttctc atcaaccgcc ataacgccaa atgcggaggc ttcttcaatc
     4621 ggtactggca tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc gataagcata
     4681 cgcgagtagt cttgcttgta gatatggtcg cccgccagga tcaccacgta ttccgcttta
     4741 taacggcgga taatgtcgag gttttgggtg accgcatctg cggtgccgcg ataccagttt
     4801 tcccctttca ttctctgctg tgctggcagc agatcgacaa actcgttcat ttcttcattg
     4861 aagaatgacc agccgcgctg aatgtgctgc accagagtgt gggactggta ctgggtgatc
     4921 acgcccatac gacggatccc agagttgatg cagttagaca gcgcaaagtc aataatgcgg
     4981 aacttaccgc cgaagtgtac ggccggtttt gctcgcttat tggttaaatc cttcaggcgg
     5041 gtaccacgtc ctcccgccag tatcagggca acagatttca atggcagctg gcgcgccaac
     5101 attaagtgat cgttcttctc taaactaacc atgactaact ccttttttat catctctgga
     5161 acacacacaa tccgtgtgca ggtccctgcc agacaaccgt aatcactggg ttatcctctc
     5221 cagcgaatgg gggaatggcg tgccactccc cagcaggtaa aacaatctct gttacctcaa
     5281 gcgtggcgtt aattgcgatc aaaaagcgat ccgagagcag aatttgcagc tgtttcggcc
     5341 cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg atttagccaa cggacattgc
     5401 cgtcaccttc ttcccaccag cgattctcca ccaaagcggg aatgcgcttg cgcagatgga
     5461 ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc aaccagttta
     5521 attggttatc ctgacagtag gcattgttat tgccatgctg gctgtgacca tgttcgtcac
     5581 cggccagtaa catcggcgtg ccctgggaga gcaacaacgt tgttaacagg gcatgaatgc
     5641 tgtcgcgccg ccgttcaacc aagtcaagag taccgcctaa cccttcttta ccatgattgt
     5701 tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca ttgtgtttat
     5761 ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg agattaatcg
     5821 cggcactcgg cagacgacca ttacgtttaa aaacatcgct ggaggcagca aaacgcccgg
     5881 caaacgcccc cagaggcaaa tcataatgta gccagaaacg acgggcagca tcgcggaaat
     5941 gatcgttcca ctcggcaaac agcggcggga aatttcccac ctgataacca ctaggagcga
     6001 tatcccacgg ttcagcaatt aacttcacct gcgagagcac cgggcagttc tggatagcgg
     6061 taaacaacgg cgcatcctga cggaactctg gcgtacggcc catgactgcc gccagatcaa
     6121 agcggaaacc atcgacgtgg caggtttcta cccaataacg caggcaggcg ctggcataat
     6181 ccaccaccgc cggatgactc aaattgagcg tgttgccgca accggtccag ttgtgataat
     6241 cgccgtcttc tcttatccaa taatagctac ggttatcgat cccacgcagc gagaataacg
     6301 ggccgtcgag gtccagttcc gcactatggt tgagcacgat atcaagaatg acttcgatac
     6361 ccgctttatg cagtgctttg attgcatcgc gaaactcatc cagcgccgtt tctggcgagc
     6421 aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag tagttactta
     6481 gccccatgcg ttgcagacgt ggttcactgg caaactgcgc cactggcagc agttccagcg
     6541 cggtaatgcc caattgtttc aaatagttga tcatcaccgg atgcccgagg gctttataag
     6601 tgccacggat ctcgaccggg atctccgggt gcaagtacgt taatcctttg acatgggctt
     6661 cataaatgat ggtgctgccc cacggcgtgc gcggcggggc gtcatcttcc cagtcatagt
     6721 gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag tcaggttcat
     6781 tatgaccggc gtgcagcagc gggttatctt taaactcccc gtcaatttgc cgcgcgcaag
     6841 gatcaatcaa caacttcgcc gggttaaagc gatgcccctc ggcgggttgc caggggccat
     6901 gaacgcgata accataacgc aaacccgggc gcgcatccgg cagataaccg tgccaaatgt
     6961 cgccactgtt ccctggcaag tcatagcgat gttcctggcc attggcgtca aagacacaca
     7021 gttctacccg ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga ccgtcgtaat
     7081 gcgcgccgag gggagcgggt ttgccaatgg cgagttgtgt cattctgcct cccgaaccag
     7141 ccagatagtg gccagcggtg gtagcgtcag gcttagtgaa tgctgacgac cgtggctggc
     7201 aatctcatcg ctgtgtaccg tgccgccatt gcctgcatta ctgccgtgat agtgtatgga
     7261 atcggtattg aggatttcac gccatttgcc tggctgatta atgccgaagc gataatcatg
     7321 ccgcggcacc ggcgtaaagt tactggcaac gataatttcg ttaccctctt tatcgcgacg
     7381 cacaaagatc agcaccgagc gttctttgtc atccaccacc agccattcaa agccgtacgg
     7441 gtcaaaatcc agttcatgca ttgctttatg gtggcggtag gtgaggttca gatcgcgcac
     7501 cagacgctgg acaccgtggt gccagttatc gccgccttcc aacagatgcc agtcgaggct
     7561 ggcgtcatgg ttccactcgc ggccctgggc aaattcgttc cccatgaaca gcagtttctt
     7621 gcccggaaat gcccacatcc agccatagta agcgcgcagg ttagcgaatt tctgccatgc
     7681 gtcgcccggc atgcggtcga gaatcgattt tttaccgtgg accacttcat catgcgacaa
     7741 cggcaggacg aagttttcag tgtagttgta gagcatcccg aaggtcagtt tatcgtgatg
     7801 atactgacga taaatcgggt cgagcttcat gtagtccagg gtgtcatgca tccagccgag
     7861 gttccacttg taccagaagc ccagaccgcc catatcctgc ggacgagaaa cgccagggaa
     7921 atcggtagac tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa gaatacggtt
     7981 ggtattacgc aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt cgttcgggat
     8041 ccactccccc tctttacggc tgtagtcacg ataaatcatc gacgccaccg catcgacgcg
     8101 cagcgcatca ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga ggaagttact
     8161 gacttcacgg cgaccatagt tgtagatcag cgtgttccag tcctgatgat agccttcacg
     8221 cggatcgctg tgttcataca agttcgtgcc atcaaattcg gcaagcgcaa agtcatcagt
     8281 cgggaagtgg cctggcaccc agtcgagaat cacgttcaga ccagctgcgt gtgaggcatc
     8341 aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat acaggccggt
     8401 tggctgataa ccccaactgc catcgaaggg atgctcgtta atgggcagta gttcgaggtg
     8461 ggtaaagccc atccatttag cataaggcac cagttgatcg gccagctcgc ggtagctcaa
     8521 ccagaaattg ttgtcggtgt ggcgacgcca ggaacccagg tgaacttcat aaatagagat
     8581 tggcgcatca aactgattcg cttttttgcg ctcttcagtc tgtacaacct tttccggcag
     8641 cccgcaaata agagacgcgg tttccgggcg catttgcgct tcgaaggcat aagggtcgga
     8701 cttcagacgc aagttgccat tggcatcaat catctcgtat ttatagagct gaccgttatg
     8761 cgccccaggg ataaacagtt cccagatgcc gctctcttta cgcaggcgca tcgggtgacg
     8821 gcgaccgtcc cagtagttga attgcccaac caccgagacc cgacgggcgt ttggagccca
     8881 gacagagaaa cgcgtacctg tgacgccatc catagtatct gcatgcgcgc ctaaggtttc
     8941 atacgggcgc aggtgagtac cttcagataa tagccaggca tccatttcct ggattagcgg
     9001 accaaaacgg taaggatcat caatcaggtt ttgctgacca tgccagacaa cagccaactg
     9061 atagcggaaa aaattcttac gtcgcggaat gacgccgcta aagaatcccc gtgagtcgag
     9121 acactccagt tttgcgagtt tgcgcccggt tttcggttca atcacccaca catcggtagc
     9181 gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt ttatgcattc ccagtacgga
     9241 aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc acgtctctat cgatacgatc
     9301 ggacatgctt gtcttcctgt tttattgtgt caccccatcc aattggattt ttgacttctg
     9361 gttgtgacat ttttttgacc tgaacggcgc agcactctgt gcatcctctc tgcgtcgtcc
     9421 tcacttcagg taaggctgtg aatactcatg tattcagcca cccttaaaga atagccaatg
     9481 ctctatttaa ctcccggtaa atcatgaaac atctgcgctt actcctgtat tacgcactaa
     9541 caggggcggc atcgcgcccc agatttaatg aataaagatt acgccagttg acgaagcatc
     9601 cgacgcagcg gctccgcggc cccccacagc agctggtcgc ccacggtaaa ggctgacagg
     9661 aactctggtc ccatattcag cttacgcagg cggcctaccg gcgtggtcag cgtgccggta
     9721 acggcagctg gggttagctc acgcatagtg atttcccgat cgttcggaac gactttcgcc
     9781 cacggattgt gcgcagccag cagttcttcg acggtcggaa tagacacatc ttttttcaat
     9841 ttaatagtga atgcctggct gtggcagcgc aatgccccga cacgcacaca taaaccatct
     9901 accggaatta cggaagatgt gttgaggatc ttgttggttt ccgcctgccc tttccactct
     9961 tcgcggctct gaccgttatc gagctgtttg tcgatccacg gaatcaggct acccgccagc
    10021 ggcacgccaa agttatccac cggcagctca ccgctacggg ttaaggttgt gactttgcgt
    10081 tcgatatcga gaatagcaga ggacggggtc gcgagttcat ctgccacatg gccatacaga
    10141 tggcccatct gggttaataa ctcacgcata tgtcgcgcac caccgccgga agcggcctgg
    10201 taggttgcaa cggacaccca atcaacaaga tcattggcga ataaaccacc caacgacatc
    10261 aacatcaggc ttacggtaca gttaccgcca acaaaagtcc tgatgccatt atttaatccg
    10321 tcggtaatga cgtcctgatt gacggggtca agaatgatga tggcgtcatc tttcatgcgc
    10381 agagacgatg ctgcgtcaat ccagtaacct tgccatccgc tttcacgaag ctttggatag
    10441 atttcgttgg tataatcgcc gccctgacag gtcacaatga tatcgagggc ctttagcgcc
    10501 tccagatcaa aggcatcctg aagtgtgcca gtggttccgc caaaagacgg cgcagcctgg
    10561 ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg ctcttcaacc
    10621 atgcgttgca tgagaacgga gccgaccata ccgcgccagc cgataaaacc aacatttttc
    10681 ataagcgttt tttcctgcaa agatgtgtgc tgtataaatg tgccggtctc ctcttggcac
    10741 atctttcacc atacaaaaag cagccaaagt cgcaagtgaa attaatcaat gatagcgaag
    10801 ccatcagtaa tgcgacttat cctgctttgt tagcacgcag aaagtccgcg gcaattatca
    10861 gggaatttga gttatgaatg aaatcatttc tgcagcagtt ttattgatcc tgattatgga
    10921 tccgctcgga aacctgccta ttttcatgtc cgtactgaaa catactgaac cgaaaagacg
    10981 gcgggcaatc atggtgcgag agttgcttat tgctctcctg gtgatgctgg tgttcctgtt
    11041 tgcgggtgag aaaattctgg catttctcag cctgcgagca gaaaccgtct ccatttctgg
    11101 cggcatcatt ctgtttctaa tcgccattaa gatgattttc cccagcgctt caggaaatag
    11161 cagcgggctt ccggcaggtg aagagccatt tatcgtgccg ttggcaattc cgttagtcgc
    11221 cgggccgact attctcgcca cgctgatgtt gttgtctcat cagtacccga atcagatggg
    11281 acatctggtg attgctctgc tgctggcctg gggcggcacc tttgtcatcc tgctacagtc
    11341 ttcgctattt ttacgtctgc tgggcgagaa aggggtgaac gcacttgaac gcctgatggg
    11401 attgattctg gtgatgatgg caactcagat gttcctcgac ggcattcgaa tgtggatgaa
    11461 ggggtaacga ggagagtttt tcgaaaagag cccggtagcg tcacaactac cgggcgaaca
    11521 aacttaattc aacagctgga acgcaatcat cccaacgatg gcaccaacag tgccgaggat
    11581 ggtttccatc atggtccagg ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc
    11641 gaacaaccag aaaccagcgt cgttaacgtg gctgacaaca atcgaaccac cagcgataca
    11701 aatcgacagt gccgccattt gcgcaccaga gtagttcagt tgttcaatga ctggcatcac
    11761 cagtcccacc gccgttaaac aggctacggt ggcagacccc tgaatgatgc gcactgcagc
    11821 agccagcacg aagcaggtga tagcaatcgg caggcccatg ccggttaacg cttcgcccag
    11881 tgccggacct acgccagagt caaccagcac ctgtttgaac acgccgcctg caccaatcac
    11941 cagtagaatg atccccgccg gttgcagcgc gtgaccgcaa atgtccatca ctttgtcttt
    12001 tggcatgccc tgacgcattg ccaggccata aatcgctacc agacaagcaa ccagaatcgc
    12061 ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg gtagagcctt ctggcacaaa
    12121 acgcgcggca atggttttca gccccaccag caccagcggc aacaggatca gcgacaggct
    12181 gaatccgaaa gatggcattt taccttcgcc gagatgcggt tcgctgatgt cgtcaggaat
    12241 atgcagctca acgtaacggc tgatgaagtt accccacagc ggcccggcaa taatcattcc
    12301 cggaattgcc gcacacaggc caatcaggat catccagcca aagtcggcat tcatctgcga
    12361 tgccagcagc attggcgctg gcccaggcac caggaacgct gccgcggccg ccacgcctgc
    12421 gaataatggg attaccagct tcaccaggtt cgtaccggtg tggcgcgcca ttgagaaagc
    12481 aacgctaatc agcagaacaa tcgccacttc aaagaacagc ggtagcgcac agaccagccc
    12541 cgcaaggccg atggcataat gcgcgcggct gtgaccgaag gatttgagca ttttgacggc
    12601 aatctgatcg actgcgccgg tttcatataa gatcttgcca aacatagctc ccagggcgac
    12661 aaccaccgcc aggaagccga gggtgcctcc catccctttt tccatcgtcg ctgcgatttt
    12721 atcgagcggc ataccggaaa aaaggccagc ccccatggac accaccatca aagccaggaa
    12781 agcgtgcata cgcgccttca tgactaaaaa cagcagcagt aaaacagacc ctactgctgt
    12841 taaaacaagc gttaatgtag tcactactta tttgcctttt ttaataacct caatggtgct
    12901 tgccacaaca ccttccagcg gttgatcgat atccaccacc agtacatcgg tttcgtccgc
    12961 acccggctcc tgcagcgttt caaactgcgt caccaacatt tgggttttaa agaaatggcc
    13021 tttgcgcgct ttcaggcgac tttcaatcac atcaaaatcg cctttcaggt agatgaaaga
    13081 gagattcgga ttaccttcac gcagcaagtc gcgatagtgc tttttcagtg cagaacaggc
    13141 gatcagcgac actttattgg tgcgctgcat agcaaacgcg gagtcgttca gcgcctgcaa
    13201 ccagggtttg cgatcgtcgt cattcagcgg ttcgccagac gccatttttt cgatattgcg
    13261 ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga agttgatgcg ccacttcact
    13321 ggcgaccgca gatttgccgc tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg
    13381 gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat taaaaatatt acgggtaact
    13441 gttatcggta acattgtcca gccggacaat atcagaagca atatccatcc gtgccttaag
    13501 tgtataagtg tgagctactt caaatttgtg ggcttaaata gatccgcccg gtgacaaggt
    13561 gaaacctaaa tctaacattt tcggtgtcac agattcgcca cgaatacgcg ccagcaggcg
    13621 ttcagcgcca atactgccca tccgctcacg cggcgtcagc acgctcgcaa gtcgtggctc
    13681 catcacctga ccaatgtcat gaccgtggaa accggcaatc gccatatcgt caggaacttt
    13741 taaccccaga cgctgacatt caaacgccgc gccgaccgcc agatcatcat tagtacagaa
    13801 cacgccatcc agctgcggat attcccgccg cgcctggcga atcagttcaa taccggaaga
    13861 gtaagaagaa gattgctcaa ccatcacgct atatggcacc aggcctgcat ccagcatcgc
    13921 ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg agacgtgcgc cgagataggc
    13981 aatgtggcga tgcccacgag caataatggc ggtagtcatc tggcgtgccg cttcaaagtt
    14041 atcaaaaccg acggcgatat cgaggcacgg cgactggctg tccatcaact ccaccaccgg
    14101 gatccccgcc acttcaatca tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat
    14161 caggccgtcg atattccagg agagcatgga ttcgagacgt tcttgctcca tttccggttt
    14221 ataaccgtag tgcgccagca tggtctgata accgtgcgcg tcggtgacgc tttcgattcc
    14281 gcgtaatact tccgcgaaaa cctggttggt gagagaaggt aacaggacgc caatcgcccg
    14341 gctggtagcg ttagagagga tatcgggcgc acgattggga atatagccca gttcatcaag
    14401 agcggcggca atcttgccgc gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg
    14461 gctgaccgtc attttggtca cgcctacacg gtcagccaca tcctgaagta cgggtctttt
    14521 ctttttcatc gtcctgaagg tacaaaagag atagattctc ttagtttaac acggacgcat
    14581 cagaaccttc ccggggaaaa cagtgcttgt ttacaattat tagatataca ctaaatcaat
    14641 tgcatcgcat tgtgctaacg acgcggcaaa accccggaag cgtgcatcag cacgcgaccg
    14701 gagcgagcga ggaagcctgc aaaaaggcag gcttcaaaga tgccgttgag ttttaaaccg
    14761 gcggcagatc gaacagtaac acttcgctgt cgctatccgc atggatggag attgcctgct
    14821 catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg gtaacattgc
    14881 cttttaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc gactgctcat
    14941 ctttcagcaa cgcccaacga tacagttcca tatcctgatg cactttcagc gaaccatctc
    15001 gcgcatccgg cgagagcacc agctgtttgc cctgcacggc atcgaagcga cgctgttcat
    15061 aacgcggcgt aatgccattt tcttcaggca tgatccagat ctgatacaga tgcagacgct
    15121 cggtgctgct tgggttgtac tctgagtgac gaatacccgt acccgcactc ataatctgga
    15181 actcacccgc cggaacctgc tctttattgc ccatgctgtc ctgatgctca acagtacctt
    15241 ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag ccctgccctg
    15301 cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa gcccataaag ttcggatcgt
    15361 aatagttagc aaaagagaaa gtatgccagg agtccagcca gccatgattt gcatgaccac
    15421 gttcatttgc tttgcgtaag tagatcattt gtattcgccc cctgaatgat ttcgatgggc
    15481 ttagtgtgga cccgatctgc ctgggatgat agagggtgaa aattgacccc tctgttcaaa
    15541 aaaattgaac tattcgaggg gccatcaaac ttaattcgcg agagtcactg tggagggaga
    15601 ggcttgctca aatccgcgtt caaggatttc cagattggta agaacttcag attccttgac
    15661 gtaatttggc gcaccgtggg tgatggtttg atacaacgca tcataaacac gcccgtaatc
    15721 gcccatctcc ggcttcatct cttctctgac cgtcacaccc tcgtcattga catactccag
    15781 cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc ggcataatat tagccttcag
    15841 gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac cgtgaacgat
    15901 aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt tcaggtcgcc
    15961 ataaaacagt tgcgcttcaa aggtgtcgtc aggattggct ttattacgca ggctgcggat
    16021 gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca tcgtatgcac
    16081 accaaggcca tagaacgcgc catcctgcgg cagtccaggt ttggtttctg ccaccgggcg
    16141 gtaatagtca aaatggcttt ccacttcgac aatctctccc agcttgccgc tttcaatcgc
    16201 ttttttcgcc gtcaggaagc aggaatcaaa gcgacgattc tgatatggcg tgacggtcag
    16261 ccctttgctt ttcgccagtg caaacagctc tttcgcctgc gcaagtgtcg gagtgaacgt
    16321 tttttcgacc agcacatttt tcccggcttc cagcgcgcgc ttcgcgtact cgaagtggct
    16381 gtccgcatgg gtgcagacaa caaccaactt aacatcggga tcgtttagca cttcgtcgag
    16441 atcgctggtg aaatggatat gagagtaaat gggagcctgt tcttccggct tcgcatggcg
    16501 acgaaaaata tgcgcgacat gccagctatc cttgcggtta agtacatacg gcagatggta
    16561 acgggtggtg cttttgccga agccaataaa ggcgcagttg atgaccatga tttcgtcctt
    16621 tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg gagtgcgcat
    16681 aacgaacaca agcactcccg tggataaatt gagaacgaaa gatcaaaaaa aaagccagca
    16741 cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc aggttctccg
    16801 cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt gtccaacact
    16861 ttttgcaaaa aaatgcattt gactcgcatt tgaaagtcaa tgatgttgaa agggacattt
    16921 accccaaaga ggacaaagga atgagtgaga tagtaatacg ccacgcagaa acacgggatt
    16981 acgagaccat caggcagatt cacgcccagc cggaggtgta ttgcaacaca ctacaggtgc
    17041 ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc atcaagcaac
    17101 tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg caacagcgcc
    17161 cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctctcgttgg aagaaccgcg
    17221 gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg ttacgggtag
    17281 atcgcattga actaaccgtg tttgtcgata acgcgccagc aattaaggtc tataaaaaat
    17341 tcggctttga gattgaaggg accggtaaga agtacgcatt gcgtaatggt gaatatgtcg
    17401 atgcgtatta tatggcgcgg gtgaaataga gaatttgcaa tctaagtttg tcatttaagc
    17461 cctcacctac cgggagggct taagatatta ataccccgcc gttaaatcat ccaccgagcg
    17521 cgggtcggat gcgccgtaca actcaccgtc cggcccaacc ataatgcttt gtgtgctgcc
    17581 catcgcctct ttcagcgcca ctttctgacc ttttgcttcc agcagcttga gcgtatccgg
    17641 gctaaaccct ttttcgacac gcagctcgtc cggcaaccac tgatggtgga aacgcggcgc
    17701 attggtcgct tcggcgacgt tcatgccata atcgatgcta ttcaccacca tttgcagcac
    17761 cgtggtgata atccggctac cgcctgggct accggtaacc agccaggttt taccgtcttt
    17821 caccacaatg gtcggcgaca tcgacgacag cgggcgtttg ttcggcccga cggcgttggc
    17881 atcaccgccc accagcccgt aaacgttcgg tacgcccggt ttggcggaga aatcatccat
    17941 ctggttatta agcagaatac cgctctcgcc cgcgacaata cccgtaccga aggtggtgtt
    18001 cagcgtatag gtcaccgcca ccgcgttacc gtctttatcc actacagagt aatgggtagt
    18061 ttgattactc tcataaggcg caagcttgcc agggcgaatc tcactggacg gcttcgcttt
    18121 tttgatatca atttgatcgg caatggattt ggcataggct ttattggtca gtgcctgcca
    18181 cgggactttg acaaaatccg ggtcgccaag atattccgag cggtcggcgt aggcgtattt
    18241 ctccgcttct gccatgattt gcatcgcatc ggcgctgcca aagccgtatt tcttcatatc
    18301 gaagttttcc agaatattga ggatttgtac gatatggatc ccgccggagg atggcggtgg
    18361 catggagtaa acctgatacc cgcgataatc gccgcttatc ggagtgcgtt cgaccgcttt
    18421 ataggccgct aaatcttctt tagtgatcaa gccaccgttt ttctgcatct cctgggcgat
    18481 ctgttccgca atcgtgcctt tatagaattc gtccgggccg ttttcagcaa tcatctccag
    18541 gctctttgcc aggttcgcct gcaccagcgt gccgcccttt ttcagcggct cgccctcttt
    18601 ccagaagata gctttactgt tttcgtgatt cggcaacact tcgctaccgt aggttttgag
    18661 atcgtcagcc agcgcgtcgt taacgataaa accatcgcgt gccagtttaa acgcgggctg
    18721 cacgactttg ttcagcggca tggtgccgta tttatccagc gccagcgaga aacctgctac
    18781 cgtacccggt gtgccggaag ccagatgcga agtgagtgat tttttgctgt ccgggttgcc
    18841 ctgatcatcg aggaacatat cgcgggtcgc tttggcgggt gccatttcgc ggaaatcgat
    18901 agccgtggta ttgccatttt tcgagcggat taacataaaa ccaccaccgc ccagattccc
    18961 tgcctgcgga tgcgttaccg ccagcgcgta gcccaccgcc acggcggcat caacggcatt
    19021 cccgccctcc ttgaggatat ccacccccac ctgagtggca gtggcgtcca cagacgctac
    19081 cattccctgt ttcgcgcgta ccggatggaa gacatcttcc tccacaccat acgagacggg
    19141 cggcgctaaa acaacttcct gagagcagag cagcaatggc cacccggcgt aaaaacgtcg
    19201 gttttatcat cgttattctc cagagattaa ggggcaaccc cagctaagcc tggtatataa
    19261 ctctgaatta atcatcgttt tgtcgggaag cgagtaaact taaaggatat cttcagagga
    19321 ggatacgcga tgaaacgact tctgattctt acggcactcc tgccgtttgt cggctttgca
    19381 cagcccatta atactctgaa caaccctaac cagccggggt atcagatacc cagccagcag
    19441 cggatgcaaa cccagatgca gactcagcaa atccagcaaa aagggatgct gaatcagcaa
    19501 ctgaaaacgc aaactcagtt gcaacagcag catttagaaa accagataaa caataattct
    19561 cagcgggtgt tgcagtcaca gccgggggag cgaaatcccg cccggcagca aatgctgccc
    19621 aacaccaacg gcgggatgtt aaacagcaac cgtaatccgg atagttcgtt gaatcagcag
    19681 catatgctgc cggagaggag aaacggcgac atgctgaatc agcccagcac gccgcagcct
    19741 gacattccgt tgaaaactat tgggccgtaa agttcggacc aatcacgtca atcgcatcgg
    19801 tacagatgca atccacgccc cagcgcagca actctgccgc gcgctggggt ttattgacgg
    19861 tataaaccag aatccgtaat ccggcgtctt tcaactgcat cactcgcgct ttatcgagta
    19921 acttatgatt gagatgaata gagacgcagc ccagccgcgc ggtcagttcg cgccagtcgt
    19981 cgcgccactc atcaagcaat aaaccacgcg gcagttccgg tgccgcctgt tgtgcagctt
    20041 ctaaagcatc aatctcaaac gatgacagca gcggcggcgt cataccggcc cacagttggc
    20101 gtgccgccag cgccaccatt ttgcccgtta atggtccagt gccagtggtg ggtttgattt
    20161 cgatattcgc catcatcccg tgttcgcgac agcgttccgc cacctgcgaa agcaacggca
    20221 gcggctcacc tttaaacgct ttgctgtacc aactgcccgc atccacgcgc agtaaatcct
    20281 gccagttcag ttcacccgcg acgccccagc cgttgctggt gcgttcgaga ttgtcgtcat
    20341 ggagcaggaa gatctcgcca tctttcgata acttcgcgtc aaattcgatc atcttatgac
    20401 cgtattttgc cccgacgtcg attgccgcca gggtgttttc cggggccagc ttaccgccgc
    20461 cacgatgagc gacgatgcgg ggataaggcc agttactcat actcgttgtc ctgtttcacc
    20521 atcaaaaaga tgcagctgat tttccgccag atgcagccac agcgtgctgc ctgccgtcgg
    20581 gcgctcctga tgcgccagtc gcaccaccag cttctgcttg cgagcttagc gcaatatgtt
    20641 ccgggcgaat gccgagggtc attttacgcc cggcatactg acggtagcca ccgtttagcg
    20701 gtagcacgat accgccgtcc agttcgaaat gcgtgccttc attattcatg cggcctgtca
    20761 gcaggttcat cgccgggctg ccgataaaac tcgccacaaa caggctggcg ggcttttcgt
    20821 agacttccac cggcgtgcca atctgttcgg caacgccgcc gttcatcacc attactcgct
    20881 gggcgagcgt catcgcctca acctgatcgt gagtaacgta gagtgaagtc gttttcaggc
    20941 gacggtgcag ctgttgcagt tcaagacgca tctgtacgcg cagcttggca tcgaggttag
    21001 agagtggctc atcaaacaag aacaccgccg gatcacgcac aatcgcgcgt cccatcgcca
    21061 cacgctggcg ctgaccgccg gaaagctcgc gcgggcgacg tttgagcaga ccgtccagct
    21121 ccagaatgcg cgccgcttct ttaacgcgct cggctatttg ctgcttgccc atgccgcgaa
    21181 ttttcagccc ccacgccatg ttttcttcga cgctcatatg cggataaagc gcgtaattct
    21241 ggaacaccat cgcaatcccg cgatctttcg gctccatttc ggtcacgcgc tggtcgttga
    21301 tccagatatc gccttcggtc accctctcca gcccggcaac catacgcagc agcgtcgatt
    21361 tcccgcagcc agacggcccg accatcacga taaattcgcc atccgccaca tcaagggtca
    21421 gcggtttaat gacctaggtt ttgccatccc agcttttggt tactgcctgt aatttcagtc
    21481 ctgccatctt atttctcact atcgaccagg ccgcgcacga aggcacgctg catcactaaa
    21541 acaatcacta ctggtgggat cagggttaac aacatcgccg ccatcactga gttccattcc
    21601 gtggtgcctt cgccagtggc gatcatccct ttgatccctg ccacggtggt gccgagatcc
    21661 acgtcggtaa taatcaacaa cggccacaaa tactggttcc agccgtaaat aaaggtgatc
    21721 acaaacagcg ccgccagatt agttttggag agcggaaaaa cgatgtcgca aaagaagcgc
    21781 attggtgacg caccgtcgat ccgcgccgct tccaccagtt catccggcag cgtcataaag
    21841 aactggcgaa acagaaacgt ggcggtcgcc gaggccatca gcggcagcgt taaaccggcg
    21901 tagctgtcga gcatcttcag gttggcgatg acttccaccg tcgggaagat acgtacttca
    21961 accggcagca tcagggtgat aaaaatcatc cagaagaaga ggttacgtag cggaaaacga
    22021 aaccagacaa tggcaaatgc cgagagcatc gagacagtaa ttttgccgag cgtaatgctg
    22081 aacgccatca caaagctgtt aagcaacatc cgccagaacg gcgcgctatt tgtgcctacc
    22141 ccgttcaccc agatgttgtg gatgttttcc agcagatgcg tgccggggat gagcgtcatc
    22201 ggcgcggcgt agacggcctg tttatccagc gtcgccgcga caaacgccac gtacagcggg
    22261 aagaggatca ccgcgatccc gaggatcagc atggtatggc tgaatatcgt cagccacgga
    22321 cggttctcaa ttattggtaa cgcaccttgc tttcaacata gcggaactgc accaccgtca
    22381 gcacgatgac gaggaacatc aacaccaccg actgtgcggc agacgaagcc agatccagtc
    22441 cggtaaaacc ttcgcggtag atcttataaa tcagcgtcgt ggtggcctga accggcccgc
    22501 cggacgtggc ggcgtcgatc accgggaagg tgtcgaagaa ggcatacacc aggttcacta
    22561 ccagcaggaa gaaactcacc ggggcgataa gcggcagcgc aatcttaaag aagcggcgaa
    22621 tcggccctgc accgtcgatg gctgcggctt cgatcaacga acggggaatg gattgcagcg
    22681 cggcatagaa gaacagaaag ttgtagctga tttgcttcca caccgaggca aacaccacca
    22741 gaaacatcgc ctgaccgcta ttttgcgcgt ggttccagtc gtagccgaac tcagcgagaa
    22801 aatgggtgat cagcccgcga ccggggttaa acaggaagat ccacaatacg gcggcaacgg
    22861 cgggagccac ggcatacggc agcaacatta aggtttggta gaaacgactg ccgcgcacga
    22921 tgtactccac cagcgccgca aagaacagcg ataccagcaa accgctgacg gtgacaaagg
    22981 tgctgaattt tatcgtcgtc cagaaggagt cgagataata gctgtcatga aacagcgtga
    23041 cgaagttatc cagaccgaca aactggctgg agaaaccaaa cggatcgacg ctttgtagcg
    23101 agtaccacaa cgcttcgccc gcaggccaga taaaaaagat aacggtgatg atgagctgcg
    23161 gcgcgaccag cagataaggc agccagcgcg agcggaacac cggacgggat gatgacatta
    23221 cggttaattc ctgaactgtg ccggatgcgc ttcgcttatc cggcctacac gcgatgccag
    23281 gtcagataag cgaaacgcat ccggcattac actgattaag acttcgtcga tttctcaaag
    23341 cggcgcagta actggttccc acgctcaact gcggtatcca gcgcctgctg tggcgtcttc
    23401 ttaccggtcc acacgctctc aagctcttca tccacaatca cgcggatctg cggcatgttg
    23461 cccagacgaa gccctttggt gaacggcaac ggcggcttgt tcagcatctg acgcgtcgcg
    23521 gtatccgcac ctgggttttt ctcgtaaaag ccctgctcac gggtcaggtc atacgccgct
    23581 ttggtaattg gcagataacc ggttttctga tgccactcag cagcgttttc tggcttcgcg
    23641 aggaagtcga gaaacttcgc cacgccggta tacgtttctt tatctttgcc ctgcatcacc
    23701 cacaggctgg ctccgccgat aatggcgttt tgtggcgcat ctttcgcatc ggcgtcgtaa
    23761 ggcatcatac ctacgccgta gttaaatttg gcgtactcgc gaatgttggc aagagaaccg
    23821 gaagaggcgg tggtcatcgc gcaatcaccg ttatagaact tctcggtgga ttcatcctta
    23881 cgaccgacgt agctgaagtc gcccttcttg ttcatctcct cgagcatggc gatgtgtttc
    23941 acctgctccg gcttgttgaa ctccagcacc gcgtccgtgc cgtcaaagcc gttgtttttg
    24001 ctggcaaacg gcagaccgtt ccaggcgcta aagttttcca gttggatcca gccctgccag
    24061 ccgctggcgt agccgcactt aataccggag gctttcagtt ttgcggaata gtccgccaga
    24121 tcctgccagg ttttcggcgg ctgttccggg tctaaccccg ctttcttgaa ggcgtctttg
    24181 ttgtaataga gaacgggagt cgagctgttg aacggctggg agagtaagtg gccggttttg
    24241 ctgtcggagt agtaaccgga aaccgtcggc acaaactgcg actcatcgaa ctgaatccct
    24301 gcctctttaa acacgtcata caccggttta atggctttcg acgccatcat ggtggcggtg
    24361 ccaacttcat aaacctgcaa aatagccgga gcgttgccgg tacgaaatgc ggcaatcccc
    24421 gcgcttaaat tctgttcgta gttgccttta taggtcggta caattttgta atccggattt
    24481 tcggcgttaa aacgttgggc cagagaatcc acctctttac ccagttcccc ttccatagaa
    24541 tgccagaacg gaatggtcgt cactgcctgt gcattcccca ttaacgccag tccgagcgcc
    24601 agtgctgaag ctgtataatg taacggtttc atcgtttatc tctcttgttg taccgaatgc
    24661 gcgaattcac gcgttttatg ctcgcggggt aacatgacat gctcgaatta cagaaaaata
    24721 acttttttgt tacatttgta agatagtaag gtgtcagaaa gatgacaagg cggtgacggc
    24781 gtgggtgagg gaaaatggga gatggggcac ggataagcgg aaaaatagaa ggtctgaatc
    24841 aaactctaca gattactcat cgtttcatgc cggatgcggc gtaaacgcct tatccggcct
    24901 acaagatcgt gcaaattcaa tatattgcaa ctcacccagt aggcctgata agcgcagcgc
    24961 atcaggcaat tttgcatttg tcacctgtct caaaggagtc ttttgactcc ttatcaatca
    25021 acgtgttatt acccgcctaa atacgcactt ctcactgctt cgttcgccag cagcgcgtcg
    25081 ccggtatcgg agagcaccac atggccgttt tccagcacgt agccgcgatc cgccagcttt
    25141 agcgcctggt tggcgttctg ctcgacgagg aagatagtca tcccctgctc gcgcagctgc
    25201 tcgatggtgt cgaaaatttg ctggataata atcggcgcaa gaccgagcga tggctcatca
    25261 agtaacaaca aacgcggatt gctcatcagc gcgcgaccaa tcgccagcat ctgctgttca
    25321 ccgccggaca tggtgcccgc ccgctgaaca cggcgctcat gcagacgtgg aaacagttca
    25381 tacacccact ttatgcgctc ctgaaactgg tcacgttcgg caaagaagcc gcccatcgcc
    25441 aggttctctt ccaccgtcat ccgcgagaag acgcgacgcc cttccgggac aatcgctacc
    25501 gcttcgcgca tgattttcgc cgtctgccag tcggtaatgt ctttatcatc aaacacaatt
    25561 cgcccgctgg tggcgcgcgg atcgccgcat aacgtgccga gcaaggtggt tttccccgcc
    25621 ccgttcgcgc caatgagagt gacaatctcg ccctgattga tatgcaggct cacctcatgc
    25681 agcgcctgga ttttgccgta gtgggcgctg actttgtcaa aggacaacat gactttttcc
    25741 atcttatgcc tcacctaaat aggcacggat cacgtccggg ttattacgga tttgctccgg
    25801 cgtaccgttt gccagtggcg tcccctgatt gaccacataa attcggtctg aaatccccat
    25861 caccagcttc atatcgtgtt caatcaacaa gatagtggtg ttgtgatgat tgcgcagttc
    25921 ggcaatcagc tcatccagct ctttggtttc ttttgggtta agacctgccg ccggttcgtc
    25981 gagcattaaa atctccggct gcgtcaccat gcagcgggca atctccagac ggcgttggtc
    26041 accataggcc aggttacttg cctgacggtt ggcgtgttcc agcaaaccaa tgcgctcaag
    26101 ccaggtggcg gcgcggtcga gcgcttcgct ctgggcgcga cggaaagaag gcgttttcaa
    26161 caggccagag aacagcccgg ttttcagttg ctgatgctgc gccaccagca ggttttcaat
    26221 taccgtcatt tcacggaaca gacgcacatg ctggaaggtg cgcaccacgc ccatgcgagc
    26281 aatttgctgc cccggtaaac cttcaagatg ctgatcgcgc agtaaaatgg tgccgccggt
    26341 gggtttgtag aaaccggtca ggcagttaaa aaccgtggtt tttccggcac cgttagggcc
    26401 gattaacgag acgatttcct gcgggtacag ttcaagattg acgttgttca ccgccagcag
    26461 gccgccgaag cgcatcatca gaccgttaac agataataat ggctgactca tgcctgctct
    26521 cctttcgctg cgccgttttt cagcttcagt tgcgggcgcg tcatgggcag caaaccctgc
    26581 ggacgccaga tcatcatcag caccatcaaa ccaccgagca ttaacatgct gtattcgttg
    26641 aaatcacgca tcaattcgcg cgacaccacc agcagaacag ccgccagaat cactgcaaat
    26701 tgcgagccca taccgccgag caccactatc gccagcacaa aggccgattc agcaaaggtg
    26761 aaggattccg ggctgacaaa gccctgacgc gccgcaaaca gcgttccggc aaaaccggca
    26821 aatgcggcgc tgatggtaaa agcggtcagc ttgatacggc gcgggcttaa gcccagcgaa
    26881 cgacaggcga tctcgtcttc acgcaacgct tcccacgcgc ggcccagcgg catccgcagc
    26941 aggcggttaa tgacaaacag gcttaacacc accagcagca acgccaccag gtagaggaag
    27001 ataacgcgat cggacggatc gtatttcagg ccaaagaaat tactgaacgt gtcccagccg
    27061 ccttcacgag cggtacggct gaactcgagt ccgaagagtg tcggtttcgg gatctgactg
    27121 attccgttcg ggccgccggt gatttcagtg ttattgagca gcaatatgcg cacaatttcg
    27181 ccgaaaccaa gggtaacgat cgccagatag tcaccgcgca gacgcagcac cggaaaaccg
    27241 agcaggaaac ccgccgccgc tgccatcagt ccggcaatcg gcaaacaggt ccagaacccc
    27301 agaccgtaat agtgattgag cagcgcaaaa gtgtaagcgc cgatggcgta aaaaccgccg
    27361 taccccagca ccagcagacc agaaagacca acaaccacat tcagcccgag accgaggata
    27421 atgtagatca tggtcagagt ggcgatatcc accgtcccac gtgacaccat aaacggccac
    27481 gccaccacaa gcaccagcag cgccacgagg aacagctttt gcttcaccgt ggacccatca
    27541 atcgcgggca gaataaactt cggcccggaa acgcttttca accctttctg gaaagccggt
    27601 cgcaaaagct ggaagaaaaa gaccaccgcc gtgccgataa acatccactg ccaacggatg
    27661 tctgaagccg tgtcgaccac cagtttggtg ccatccagct ccagttgtac gcccataaag
    27721 acgcccgcca ggacaaagaa catcgcggca gagagcagtg ccattgcaat atgcatcggt
    27781 ttcatacttt ctctacctcc gggcgaccca gaataccggt cggcatcacc agcagcacca
    27841 gaatcagcag ggcgaatgag accacatctt tatattccgt actcagatag gcagaagaga
    27901 gcgcctccgc aatccctagg atcagcccgc caatcatcgc ccctggaatg ctgccaatcc
    27961 caccgagcac cgcagcggta aaggctttca tcccggccat aaagccgata taggggttaa
    28021 tgacgcagta gaactgaccg agcagtacgc cagccactgc cgccatcgcc gcgccaataa
    28081 caaaggtcaa agcaatcacc cggtcggtgt tgatgccaag caggctcgcc attttcagat
    28141 cttccgcgca ggcacgacag gcgcgcccca tacgggaata gcgaatgaaa atcgtcagcg
    28201 ccagcatggc gaggaaggtg acaatccaga tcaccgcctg catggtggta atagaggcag
    28261 agaagttttc gctatggccc accacccact gaccgttaaa caggctcggc agcgccacgt
    28321 cgcgcgaacc ttcggtcagg ctgacgtagt tttgcaggaa gatggacata ccgattgcgg
    28381 aaataagcgc aatcaggcgc ttagagttac gcaccgggcg gtaagctacc cgttcgatac
    28441 tccagccgta ggcgctggcg atgacgattg cgccgacgaa tcccgccgcc accagcagcc
    28501 agccggtatc aatgcccatc atcatcagcg cggcgatgat cataaatgag acgtagctgc
    28561 caatcatata aacctcgccg tgggcgaagt tgatcatgcc gataatgccg taaaccatgg
    28621 tgtagccgat ggctatcagc gcgtaggtac tgcccagcgt gacgccgtta aacatctgct
    28681 gcaagaaata caaaaactgc tcagacataa ggtaaccttt ctaaacccgc ccgcatttta
    28741 cgggcggtgg gatgatcact tggctgccgt ggatgaaccg tcggcgtgcc actggaagac
    28801 accaaaatca aatcccttaa gatcgccttt ttcatcccag ttcagcggcc caatcacggt
    28861 gtttgcaccg ttagctttta aatctttcac cagcgccagc ggctcatcgc tgccggtacg
    28921 ctcaagggca gttgccagag attgcactgc cgcgtaagtg atccagacat aaggcccgga
    28981 cggatctttc ttgtctgctt tcagcgcatc aacgatgccc tggtttgccg gatcctggtc
    29041 atagcgtttt ggcatagtga ccaacatgcc ttcggcggca tcaccggcaa tgttcgacag
    29101 cgatgcgtta ccgacacctt ctggccccat aaactgggtt ttcaggccaa cggaacgggc
    29161 ttggcgcagc atctgcccca tttccgggta gtaaccgccg tagtaaacga agtcgatatt
    29221 ttcttttttc aggcgggcga tcagcgcgga gaaatctttc tccccggcgg taataccgtc
    29281 gaagaagacg acgttggcgt tagccgcttt cagcccgtcc tgcaccgaac gcgccagccc
    29341 ttcgccatac tgttgtttgt cgtgaatgat ggcgatgcgc tggggcttca ccgtctcaag
    29401 aatgtatttt gccgccgtag gcccctggga agagtccagt ccggcagtac gcataatgtg
    29461 ttgataaccg cgttgggtca gctccgggtt ggtcgctccc ggcgagatca tcagaatacc
    29521 ttcgtcttcg tagatatccg acgctggctg ggtagaggaa gaacacagat gaccaataac
    29581 gtatttaatg ccgtcgttaa cgattttgtt ggcgaccgca acggcttgtt tcgggtcgca
    29641 ggcgtcgtca tattccacgc caaccagctt gtcgccctta attccccctt tgacattaat
    29701 gtctttaatc gcctgacgcg cgccgttaaa ttccatatcg ccccactggg caatcgggcc
    29761 ggacatcgcg ccgacaacgg cgactttaat atcgtcagcc atagcggtgt gtgaaattgc
    29821 cagtgcaatc atccctgcga tgatagtttt cgcattccgt ttcatagtca aaaatcccca
    29881 ttcgtgatgt tgtgttgctt tgtttttatg tgttaacaaa tcagactgtt ctttttttat
    29941 actgcactgt ttttgcctgt ctgattaagg ggttagcgca gtattttgtg atgatagcga
    30001 ttaaaatccc tatttttcag tcgattaaga acagataata ttctgaattt attgatagat
    30061 aaacagaaaa aagtgccttt gtcagcataa aataacggca caaagggcgg aataattcac
    30121 tatcattcag gggattatgc tggacatttt tcattctcta atgttttaac tttgtaatta
    30181 ttgctgttaa aaaattaatc acctgccaaa agaaataaaa aagagaaagc ctccgattaa
    30241 attatttcgc tacactggtt ccacttttgt gatttacacg ggttacccat gaagctgacc
    30301 atcattcgat tagaaaactt tagcgaccag gaccggattg acctgcaaaa gatctggccg
    30361 gagtattccc cttcctcttt acaggttgac gataaccacc gtatctacgc cgcgcgtttt
    30421 aacgagcgtc tgcttgcggc cgtgcgggta accttaagcg gcaccgaggg agcactggat
    30481 tctctacgcg tgcgggaagt cacccgccgt cgcggtgtgg ggcaatatct gctggaagag
    30541 gttttgcgta acaatcctgg cgtttcatgc tggtggatgg cggatgcagg cgtggaagat
    30601 cgcggtgtga tgacggcgtt tatgcaggca ctgggattta cggcacagca gggcggctgg
    30661 gagaagcgtt aatcgtcaag ttgatttcaa aagtgatgac caacgagaaa ttgcctgatg
    30721 cgctacgctt atcaggccta taatgtgtgc tgcaatttac tgatttcttt ggttcttgta
    30781 ggccggataa ggcgtttacg ccgcatccgg catgaagcaa cgtattcgat attagcaatt
    30841 tggcggcaac ccaaagttgc cgattaacaa ttacttcgca tcggtcgccg tgccgttggc
    30901 gtgccagtca aatacgccga actcaaagcc ctttagatcg cctttctcat cccaggtcag
    30961 tggccccatc acggtatcca cggagttcgc ttttaggtat ttggcgattt cagccggatc
    31021 gtcagactga ttcaggcccg cctgcaaaga ttgcagcgcg gcgtaggtgg tccagacgaa
    31081 tgcgccgctt gggtcctgtt ttttcgcttt gatcgcatca acaatgggtt tgttcgccgg
    31141 aacctgatcg tagttcttcg gcttggtcac cagcagccct tccgctgatt cgcccgcaat
    31201 gttagacagc gaaacgttag ccacaccttc cggccccata aactgagttt tcagccctgc
    31261 cgcgcgtgcc tgacgcagga tttgccccat ttccgggtga taaccgccgt agtaaacgaa
    31321 gtcgatattc tcttttttca gacgcgccac cagcgttgag aaatcttttt ccccggcggt
    31381 gataccatca aagaacacca cgttagcatt gcctttcttc aggccgtcct gcaccgctcg
    31441 tgccagacct tcgccgtatt gctgtttgtc gtgaacgata gcaatacgct gcggtttcac
    31501 tttctcaaga atatatttcg ccgccgtcgg cccctggtcg gagtccaggc cggtggtgcg
    31561 caggatcagc tgatagccac gggcggtcag ctccggcgcg gttgccgctg gggtgatcat
    31621 taaaatgcct tcgtcttcgt agatgtcaga cgcaggctgc gttgatgaag aacagaggtg
    31681 accaatcaca tatttaatac cgtcgttaac gactttgttc gccaccgcaa ccgcctgttt
    31741 cgggtcacag gcatcgtcat attttacgat ttgcagtttg ttgcctttaa tgccgccttt
    31801 agcgttgata tccgcaaccg cctgctctgc gccggtaaac tcctggtcac cgtactgcgc
    31861 aaccggaccg gacattgcac ccacgaccgc gactttaata tcttctgcca gagccatatt
    31921 gctgaatgcc agcgcgatac atcctgccag taacgcttta ccctttatgt tcatcctgag
    31981 aatccccatt cttctggtta ttacgtgtgt tgtgatgttg ttgttcagca ctttatttcg
    32041 ttttatgcat gactacccgt gctttagcag catactctgc taaaacatac ccgattttta
    32101 tgatattgga atagctattt tgacagttta ttaacaatct gcgtggggat tggcgttttg
    32161 ccggaggggg aattgattat tgtagaggcc caaaaaacaa aaaccccgga ctctcatcca
    32221 gggttctctg cttaatagcg gaaattacgc ttcaatagcg gcgcgaagtt ttttcatcgc
    32281 gttcttttcc agttggcgca cacgttcagc ggaaacaccg taacggtcag ccagttcctg
    32341 caacgtggac ttgttgtctt cgtccagcca gcgcgcacgg atgatgtcct ggctgcgttc
    32401 gtccagacca tgcatcgcgt cggtcagacg gtttgccgcc tgctcttccc agttatcatc
    32461 ttcgatgccg tcggcaaagt tagatgattt atcctgcaga tagagcaccg gtgccatcgg
    32521 ctggctgtcg gaatcgtcgt cggaagacag gtcaaaggtc atgtcctgtg ccgccatacg
    32581 tgattccatc tcacgtacgt ctttgctggt tacgcccagt tcacgggcca ccatttcgac
    32641 ttcatcctgg ttaaaccagc ccagacgctg cttggtttta cgcaggttga agaacagttt
    32701 gcgctgcgct ttggtggtcg caactttgac gatacgccag ttacgcagaa cgtattcgtg
    32761 gatctctgct ttgatccagt ggacggcgaa ggagaccagg cgcacaccca cttccgggtt
    32821 aaaacggcgc actgctttca tcaggccgat gttaccttcc tgaatcaaat ccgcctgtgg
    32881 caggccatag cccgcataat tacgagcaat atgaacaaca aaccgcaggt gagacaggat
    32941 cagcgtttta gctgcttcca gatcgccatg gtaatgcagc ttttcagcca gcgcccgctc
    33001 ctcgtcagcc gacaacatcg gccacgcgtt agctgcccgg atgtaggaat ccaggttgcc
    33061 aactggggct aaagctaaac tttgcatttt gtcagtcatt caaatcctct caatcgatat
    33121 cttctggcgc ttcagtggta gcaacaactg tgccaaagct taagagcaac gaggttatca
    33181 ttcactgttt tatcagaccg tgattttatc cacaagttca atgcaagctt gtgaataaat
    33241 tacgcacaaa atgtgacata gagatgaaat accgggaaga gacaacgggg tctctttccc
    33301 tgctacggaa cccattgcag ggaaagagta taacacgctt ttattattca ggcgtaaagt
    33361 ggcgtaaatg ttgtaccgtg gcaagccacg ctgccaccca gccaatcatc gagcatacca
    33421 gcagcaatag caggcattca tcgaacgata aaccattgat atcaaacttc gttccgaaaa
    33481 cctgtgccac ttccgccacc gctgacgaca aacgcagtac cagaatttct gacaaaatta
    33541 atgacaacaa tgcgccagaa aatcccagca gcgcaccacc gtacaggaac gggcgaagga
    33601 tgaaaccatc cgtcgcacca atcagtttct gcacgttgat ggagtcacgg cgagcaaaga
    33661 tgctcagacg tacgctgttg ccgatgacga ggaacactgc cgccaccatc aacacgccga
    33721 tcatcgccga aacgcgccca accagcccgg tcaacgccgc cagacgggca aaccagctgt
    33781 catccatccg cacttcgtca atgccgttaa tctgcgtgat acgatcacgc agcgtattga
    33841 gtgattccgt cccctgaaaa tcgagtttcg ggatcaccac cgccactgcc ggaagcgggt
    33901 tttcttccag catatccagc gcaccaccaa aaccagacca gttacggaac tcgcccagtg
    33961 cgtcttcacg agaaagatag ttcactttct ccacgccttg ctcggcctgc aactgtgcca
    34021 ccacgcccgc agcggcgtca tcgtccagcg ttttttgcaa gtaaacagtg atttgcggtg
    34081 acggataata ctgcgtcgcc gcctgattaa cgtttttgta caccatataa cagacgctgg
    34141 gcagcgtcag ggagatggcg ataaccatca ccgttaaaaa cgtggcgaac ggtttgcttt
    34201 tcagatcctg caatgcgccg tggaaggcat agcgcacctg ttcgttgaaa acgttggttt
    34261 tgcgatttac cggttttggc gaggatttcg cgcgtttcgg tgcgttacga ccgccgtcgc
    34321 ctgagccgcc gaccgattta cggaagcgat ccagacgccc accaaactgc cgaatatgat
    34381 tgattgcatc gcgcttattc atggcccacg cctccatgca agtgaccatc gctcagggtg
    34441 agcatgcgat aggaacgccg tgaaatcagg ttaatgtcgt gcgttgccat caatacggtt
    34501 accccaacgc ggttaaattc ttcaaacaga cgtagaatcc cttccgacaa cgcgtcgtcc
    34561 aggttaccgg tcggttcgtc cgccagcagt accgcgggct tattcaccac cgcgcgggca
    34621 atgccaacac gctgttgttc accgccggaa agctgaatag ggaagttctt cgctttgtcc
    34681 agtagcccga ctttatccag tgccgccgac acccggcgac gaatatcgtc accgctggca
    34741 ccagcgataa tcagcgggat cgccacgtta tcgtagacag tacggtccat cagtagatgg
    34801 tgatcctgga aaatcatgcc aatctggcgg cgcagaaacg gaacttcacg gtttttcaga
    34861 cgcgtgatgt catggccgct aaaccagatt ttcccggcgc tgggccgctc aatcccacag
    34921 atcagcttca ggagggtact tttccctgcg ccggaatgac cggtcagaaa cgccatctca
    34981 cccggctgca tatggaacgt aacgccctgc agcgcctgtc tcccaccgag ataagccttg
    35041 ctgacatgtt caaagcgaat cattgttaat cctctcgggc aaaaagtgcc tctataaagt
    35101 cgtccgcctt aaacggacgc aaatcctcaa tacgttcgcc gacaccaatg tagcggatag
    35161 ggataccaaa ctggtcagcc accgagaaaa ttaccccgcc tttcgccgtg ccgtccagtt
    35221 tcgttagcgt gatgccggtt aagccaacag cttcgtggaa cagtttggcc tggcttaccg
    35281 cgttctgccc ggtgctggca tcaatagtca gcataacttc atgcggcgct tcaacgtcga
    35341 gtttcttcat cacgcggacg attttcttca actcttccat cagatgcgat ttgttctgca
    35401 gacgtccggc ggtatcggca atcaggacgt cgatattacg cgctttagct gcctgaatgg
    35461 cgtcgaagat aacagaggcg gaatccgcac cggtatgctg ggcaatcacc ggaatattgt
    35521 tgcgctgacc ccagacctga agctgttcaa ccgcagctgc acggaaagta tcgcccgccg
    35581 ccagcatcac cgatttaccc tgctgctcaa actgacgcgc cagcttacca atcgtcgtgg
    35641 ttttacccac accgttgacg cccaccatca ggatcacgaa cggcgtttta tcttcaacat
    35701 tcagcggctc atcgactttc gccagaatct cacccatctc ttctttcagc aggccataga
    35761 gcgcctcggc atcacgaagc tgcttgcggg atgcgccttc cgtcagattg gtgataattt
    35821 tacgtgtggt ttccacgccc acatcggcga tcaacagctg ttcttccagc tcttcaaaca
    35881 gatcatcgtc gattttttta ccgcggaaca ggctgataaa tccggaaccg agattttctt
    35941 tggtttttaa caggctgcgt ttcaggcgcg cgaagaagcc ttctttggtc ggtttttctt
    36001 gctcctgggc aatttcttca accggctgtt cttcttctgc cggaggaact accatcaccg
    36061 cctcttctgc cgcttcggca gccagcgcct gtgcttccag ctcttcgtcg gtgatttctt
    36121 ctttagccgc ttcttcttct gccgcttcga caatctctac ggtttccgct tcagcctgcc
    36181 actcttctgg cgaaacctct tcggcgttga cgtcttccgg caacggcagc tcttcacgtt
    36241 cgatagccac tggctccggc gtttcttcta cgaccggttc cggctgtgca acaacttccg
    36301 cttcaggctg cgctttttca ctttcaacaa cctgttcagt gacttccaca acgtcggcag
    36361 caaaagtttc cgcctcggct tcagtatgcg cctgcggctg ctcttcaacg gcttgttcag
    36421 aagccttcgc aggctcttgc gcctgaacga tttcttctac aaccggttgt tcattctgaa
    36481 cttctgtctc tttttccggg gtctgctctt tttgaccaaa gcccagccag gaaaaaaagc
    36541 cacgtctttt ttctttcgcc atttgcgact acactcctcg ctgttgcttc atggcacagc
    36601 gttaacgcta tgtacatggc agctaaaaaa atgatgaaat agtctatcac ttaacttaat
    36661 tcacatcacc gcctgcaagt atatgttatc tggcggattg agcaatttat catgaaaaaa
    36721 ccgaatcatt ccggcagcgg ccaaatccgc attattggcg ggcagtggcg aggccgtaaa
    36781 ctcccggttc ctgatagccc aggtctgcgc cccaccaccg accgcgtacg cgaaacgttg
    36841 tttaactggc tggctccggt cattgttgac gcccaatgtc tggattgctt cgccgggagc
    36901 ggcgcgctgg ggctggaagc gttatcgcgc tacgctgcgg gggcaacgtt gattgagatg
    36961 gatcgcgtgg tttctcagca gttaattaag aatctggcga cactaaaagc aggcaatgca
    37021 cgcgtggtga acagcaacgc aatgtcattc ctggcgcaaa aaggcacacc gcataatatc
    37081 gtgtttgtcg atccaccgtt ccgccgtggc ttgttagaag agacgataaa tttactggaa
    37141 gataacggct ggctggctga cgaagccctg atttatgtcg aaagcgaagt cgaaaacggt
    37201 ctgcccactg ttccggcaaa ctggtcatta catcgggaaa aagtggcggg tcaggtggct
    37261 tatcggctgt atcaacacga agcacaagga gaaagtgatg ctgattaata ttggtcgttt
    37321 gttaatgctc tgcgtttggg gatttttaat cctcaatctg gtgcatccct tcccgcgccc
    37381 gctaaatatt ttcgttaacg tggcgctgat ttttaccgtg ctgatgcatg gtatgcagct
    37441 ggcgctattg aaatccactt taccgaaaga tggcccgcag atgaccaccg ccgaaaaggt
    37501 gcggattttc cttttcggcg tgtttgaact gctggtctgg cagaagaaat ttaaagttaa
    37561 aaaataacct tattgttcgc caacaaagct aacaaagcgc gttcctttat agctcagcgt
    37621 acctttatcg cccacggtca gggcgtggta ctgctgggcg tcgaggcgaa acgtctgctc
    37681 cattcctccg ctttgcggtt tgaagcttgc ctcatagcgc atactggtgc ctgccggagt
    37741 cacttcctgc tggcgcgaac ggcgatcgtt aatcggtttt tcccgcttgt tgctcaccac
    37801 caccagcttt tgctggagcg gagccatatc attatcagct ttttcccgtc gctgttgcat
    37861 aaaacgaaac gatgcggcga cgacaattaa gccaatgata acaataaaga aaagaggtgg
    37921 tttactcatc tttatccctc atcggaaaat gcggaattaa gcataccctg ccagttatgg
    37981 tgttgtcatc cgtccaccct cgccactaaa ctggaagcaa gaccgtaggc attccgctta
    38041 cgaaaaaata acgaattcaa ggaactaaga tgctttggtc gtttatcgct gtctgtcttt
    38101 ccgcatggct atctgtggat gcatcgtatc gtgggccaac ctggcaacgc tgggtgttta
    38161 aaccgttaac ccttcttctc ctgctgttac tggcctggca agcaccgatg ttcgacgcca
    38221 ttagctatct ggtgctggcg ggactgtgcg cctcactgct gggcgatgcg ctaaccctgt
    38281 tgccacgtca acgtctgatg tacgccatcg gcgcgttttt cctctcgcac ctgctgtaca
    38341 ccatctattt cgccagtcag atgacgctct ctttcttctg gcctctgcca ctggtgttgc
    38401 tggttctggg tgcgctgcta ctggcgatta tctggacgcg gctggaagag taccgttggc
    38461 ctatctgcac gtttatcggc atgacgctgg tgatggtgtg gctggcaggt gaactgtggt
    38521 tcttccgtcc gaccgctccg gcgctctctg cgtttgttgg cgcttcgttg ctgtttatca
    38581 gtaactttgt ctggctgggg agccactatc gccgacgctt ccgtgcggat aacgcgattg
    38641 ctgcggcctg ctactttgcc ggtcacttcc tcatcgtccg ctcactgtat ctctgataaa
    38701 acttgactct ggagtcgact ccagagtgta tccttcggtt aatgagaaaa aacttaaccg
    38761 gaggatgcca tgtcgacacc tgacaatcac ggcaagaaag cccctcaatt tgctgcgttc
    38821 aaaccgctaa ccacggtaca gaacgccaac gactgttgct gcgacggcgc atgctccagc
    38881 tcgccaacgc tctctgaaaa cgtctccggc acccgctata gctggaaagt cagcggcatg
    38941 gactgcgccg cctgtgcgcg caaggtagaa aatgccgtgc gccagcttgc aggcgtgaat
    39001 caggtgcagg tgttgttcgc caccgaaaaa ctggtggttg atgccgacaa tgacatccgt
    39061 gcccaggttg aatctgcggt gcaaaaagcg ggctattccc tgcgcgatga gcaggccgcc
    39121 gacgagccac aagcatcgcg cctgaaagag aatctgccgc tgattacgct tatcgtgatg
    39181 atggcaatca gctggggtct ggagcagttc aatcatccgt tcgggcaact ggcgtttatc
    39241 gcgaccacgc tagttgggct gtacccgatt gctcatcagg cattacggct gatcaaatcc
    39301 ggcagctact tcgccattga aaccttaatg agcgtagccg ctattggtgc actgtttatt
    39361 ggcgcaacgg ctgaagctgc gatggtgttg ctgctgtttt tgattggtga acgactggaa
    39421 ggctgggccg ccagccgcgc gcgtcagggc gttagcgcgt taatggcgct gaaaccagaa
    39481 accgccacgc gcctgcgtaa cggtgagcgg gaagaggtgg cgattaacag cctgcggcct
    39541 ggcgatgtga ttgaagtcgc cgcaggtggg cgtttgcctg ccgacggtaa actgctctca
    39601 ccgtttgcca gttttgatga aagcgcccta accggagaat ccattccggt ggagcgcgcg
    39661 acgggcgata aagttcctgc aggagccacc agcgtagacc gtctggtaac gctggaagtg
    39721 ctgtcagaac cgggtgccag cgccattgac cggattctga aactgatcga agaagccgaa
    39781 gagcgccgcg cccccattga gcggtttatc gaccgtttca gccgtattta tacgccagcg
    39841 attatggccg tcgccctgct ggtaacgcta gtgccgccgc tgctgtttgc cgccagctgg
    39901 caggagtgga tttataaagg tctgacgctg ctgctgattg gttgcccgtg tgcgttagtt
    39961 atctcaacgc ctgcggcgat aacctccggg ctggcagcag cagcgcgtcg tggggcgtta
    40021 attaaaggcg gtgcggcgct ggaacaacta ggtcgtgtta cccaagtggc gtttgataaa
    40081 accggtacgc tgaccgtcgg taaaccgcgc gttaccgcga ttcatccggc aacgggtatt
    40141 agtgaatctg aactgctgac actggcggcg gcggtcgagc aaggcgcgac gcatccactg
    40201 gcgcaagcca tcgtacgcga agcacaggtt gctgaactcg ccattcccac cgccgaatca
    40261 cagcgggcac tggtcgggtc tggcattgaa gcgcaggtta atggtgagcg cgtgttgata
    40321 tgcgctgccg ggaaacatcc cgctgatgca tttgctggtt tgattaatga actggaaagc
    40381 gccgggcaaa cggttgtgct ggtagtacgt aatgatgacg tgctgggtat cattgcattg
    40441 caggataccc tgcgcgccga cgctacaact gccatcagtg aactgaacgc gctgggggtt
    40501 aaaggagtga tcctcaccgg cgataatcca cgcgcagcgg cggcaattgc cggggagctg
    40561 gggctggagt ttaaagcggg cctattgccg gaagataaag tcaaagcggt gaccaagctg
    40621 aatcaacatg cgcccctggc gatggtcggt gatggtatta acgacgcgcc agcgatgaaa
    40681 gctgccgcca tcgggattgc tatgggtagc ggcacagacg tggcgctgga aaccgccgac
    40741 gcagcattaa cccataacca cctgcgcggc ctggtgcaaa tgattgaact ggcacgcgcc
    40801 actcacgcca atatccgcca gaacatcacc attgcgctgg gactgaaagg gatcttcctc
    40861 gtcaccacgc ttttagggat gaccgggttg tggctggcag tgctggcaga tacgggtgcg
    40921 acggtgctgg tgacggcgaa tgcgttaagg ttgttgcgca ggagataagg caaaccgatc
    40981 gcaacattga gcgcgatcgg tcccctcgcc cctctgggga gagggttagg gggtgaggaa
    41041 aaggcggcat cgaagccaat cagcccctat caaccgcctt tacgaatcaa ataacgataa
    41101 ggcagtccat ccgtctcttt cgcaaccagt tcgtgttcca taaaggtaca aaacccagga
    41161 atatcgcggg tagtggccgg atcgtcggcg ataatcagca acgtttcgcc aggctgcata
    41221 ttgcgcacgg ttttgcgcac catcatcacc ggttccgggc agcgcaggcc aagcgcgtcg
    41281 agtgtgtggt cagggctgga aaagagatcg gtcattttct tctcatcact taaaaaaacg
    41341 gcgctagttt acgccctgtg agtccgtaag caaaccaggt taacgattgc gtgaaaatta
    41401 accattgcat tgtcaacgta aagcagtatc atgcggcggc tcgaaaaaag ggtaagcacg
    41461 ttattatgtt aaggtaacag acgtgtcgta cgtattgggt tccctcaccc caatggttaa
    41521 tcaaaaaggt acaatatgaa cgttttctcg caaactcaac gctataaggc gttgttctgg
    41581 ttatcgttat ttcatcttct ggtgatcacc tccagtaact atctggttca gcttcccgtc
    41641 tccatttttg gtttccatac cacctggggc gcgtttagct ttccgtttat ttttcttgct
    41701 accgacctga ccgtgcgtat ttttggcgca ccgctggccc gacgcattat cttcgcggta
    41761 atgatccctg cgttattaat ctcctacgtc atctcgtcgc tattctatat gggttcctgg
    41821 cagggattcg gcgcactcgc ccacttcaac ctgtttgtcg cccgtatcgc caccgccagt
    41881 ttcatggcct acgcgctggg gcaaatcctc gacgtgcacg tttttaaccg cctgcgtcag
    41941 agtcgccgct ggtggctggc accgacagcg tccacactgt tcggtaacgt cagcgacacg
    42001 ctggcctttt tcttcattgc cttctggcgt agcccggatg cctttatggc tgaacactgg
    42061 atggaaatcg cgctggtcga ttactgtttc aaagtgttaa tcagtatcgt tttcttcctg
    42121 ccaatgtatg gcgtattact caatatgctg ttgaaaagac tggcagataa atccgaaatc
    42181 aacgctttgc aggcgagtta aaggttcgta atcagagttg tgataagatg gatgaatgag
    42241 ccgttatggc cgtttatcga aaggaagaag tcaatgcgca atctggttaa atatgtcgga
    42301 attggcctgc tggttatggg gcttgcggcc tgtgatgata aagacactaa cgctacggcg
    42361 cagggttcgg tcgcggaaag taacgctacc gggaatcccg tcaacctgct tgatggcaag
    42421 ttaagtttct cgctgccagc ggatatgacc gaccagagcg gtaagctggg aacgcaggcc
    42481 aataacatgc atgtctggtc cgacgccacc gggcagaaag cagtcatcgt catcatgggc
    42541 gatgatccga aagaagatct ggcggtgttg gcgaagcgtc tggaagatca gcaacgtagc
    42601 cgcgatccgc agctgcaagt ggtaaccaat aaagccattg agctgaaagg tcacaaaatg
    42661 cagcagttag acagtattat ctccgcgaaa ggccagacgg cgtactcttc cgttattctg
    42721 ggtaacgtgg gtaatcaact gctgaccatg caaattacgc tgccagctga cgatcagcaa
    42781 aaagcgcaaa ccaccgcaga aaacatcatt aatacgctgg ttattcagta agttttaaga
    42841 tgatgaggcg gcctcaggga cgtgttccgg aggccgtttt tttaatcgcc acgtcagtaa
    42901 taacgcgatt gcgaccagtc ccgccgccgc cagataaatc accggtacgc ccgcccagct
    42961 catcaccagc ccagccagtg gtccagtcac gccaagcgat aaatccataa atacggtgta
    43021 agttgccagc gccgccccct aattttgctg cggaaccgct tttaccgcca ctacacccaa
    43081 tgccgggaac accagcgaaa acccggcccc cgccagtaag acgccgattt tcgccatcca
    43141 cggcatagtc gccacgccaa ccagtagcag gccgattatc tcaacgctaa agcaaatcat
    43201 cgctacgttt aagccaccga tacggttaat gccgttaggg aataacaaac gcgtaccgac
    43261 aaacgcacag ctaaacagcg tcagcgcgaa agccgcaccg tcccaacctt tagcgtcata
    43321 aaacagcgtg ataaaggtgg cgatgacgcc aaatccggcg gaagccagtg ccagcgccat
    43381 accgtacagc cagacgcgcc caagcaccgc gcgaaacggc agcggtttgc ctttactggc
    43441 ttttaccgtc ggacgcggga tcgccaacaa aatggccacc agcgccacgc ccataatgat
    43501 taacgctaac gcctgcaagc cgccccagtg ataaaacacg acgcctaacg gcgcacccat
    43561 cgccatcgcc ccgtaagtga caatgccgtt ccacgaaatc acccgcccga tatgcagcga
    43621 gccaaccact ccaacgcccc atagggtcga tcccgttccg gcaaaacttt gcccaatccc
    43681 aaggatgacg cgccccaggc aaagtaataa caggctgatg acaggcagac tggcggttaa
    43741 tcctgccgtc agatacccca gaccgctcaa aaagcagccg cataaaccga agacgacaat
    43801 ctttttgggt cccagcaaat cggcgtaacg tccggcatga gggcggctca gcaaggtggc
    43861 gaaatattgc aggctgataa ccaatcctgc ccagaaggcg ctaaagccca tcacatcatg
    43921 gacatagccc ggtaatacag cgagcggcaa cccgatggtg aggtagctgg cgaagttaaa
    43981 catgacaatg gagacaatgc gcaaattcag gcgcaatccg tttagcgcgg gttcggctac
    44041 gggttcgggc atgaggatca ccacattttt acaacagtgt ttcattttta ccatgtgctg
    44101 acgtgaaaat cagcagtaag aatcagaata ttgctggcgt gactcccgct acacttaaca
    44161 caaaaagtca caaggaagcc ccaatggaaa cccctcaacc cgataaaacg ggcatgcaca
    44221 ttctgctcaa gttggcctcg ctggtagtga tcctcgcggg cattcacgca gcggcagata
    44281 tcattgtgca gctgttactg gcgctgtttt ttgccatcgt cctcaacccg ctcgttacct
    44341 ggtttattcg tcggggagta caacgccccg ttgccattac gattgtagtg gtggtgatgc
    44401 tgatcgcact aaccgcgctg gtcggcgtac tggcggcatc gtttaacgaa tttatctcta
    44461 tgctgccgaa gtttaataag gagctgacgc gcaaactttt taaattgcag gagatgttgc
    44521 cttttcttaa tttgcatatg tcgccggagc gaatgctgca gcggatggac tcggaaaaag
    44581 tggttacctt caccacagcg ctaatgaccg ggctttccgg ggcaatggcg agcgtgcttt
    44641 tgctggtgat gaccgtagtt tttatgctgt ttgaagtgcg ccacgtccct tacaaaatgc
    44701 gttttgcgct gaataaccca cagattcaca tcgcaggatt acaccgcgca cttaaaggcg
    44761 tttcgcatta tctggcgtta aagacgctac tcagtttatg gacaggtgtc atcgtctggc
    44821 tggggctggc gctaatgggc gtacagtttg cgctgatgtg ggcagtactg gcgtttttgc
    44881 tcaactacgt gcccaatatc ggcgcggtaa tttccgccgt accgccaatg attcaggtgc
    44941 tgctgtttaa tggtgtttac gaatgtattc tggtcggcgc attgttttta gtggtccata
    45001 tggtcatcgg caatatttta gaaccacgga tgatgggcca tcgcctgggg atgtccacca
    45061 tggtggtatt tctttcattg ttaatctggg gatggctgct cggcccggta gggatgctac
    45121 tttcggtgcc attaaccagc gtgtgtaaaa tctggatgga aacgacgaaa ggtggcagca
    45181 aactggcgat tttactgggg ccggacagac cgaaaagtcg gttaccggga tgaggcgaca
    45241 agtgatacga tacgcacttt cattttccat taaacgttgg ccctgatatg tatcggatag
    45301 ttctggggaa agtttcgacc ttaagcgcag ctccactgcc accgggttta cgcgagcaag
    45361 caccgcaagg tccacgacgc gaacgctggc tggcggggcg tgcattgctt tcgcacacgc
    45421 tttccccgct accggagatc atctatggcg aacaaggcaa acctgcattc gcgccggaaa
    45481 cgccgctatg gttcaactta agccatagcg gtgacgatat cgctctgctg ttgagcgatg
    45541 aaggcgaagt cggctgcgat atcgaagtga ttcgcccgcg cgccaactgg cgctggctgg
    45601 cgaacgccgt gttcagcctc ggggaacacg ctgaaatgga cgccgtgcat cctgatcagc
    45661 aactggaaat gttctggcgc atctggacgc gcaaagaagc catcgttaaa cagcgtggcg
    45721 gcagcgcctg gcaaatcgtc agcgtagata gcacctatca ctcctcgctg tcagtcagcc
    45781 attgtcagct agaaaattta agccttgcga tctgcacccc cacccccttt acgctcaccg
    45841 ccgacagtgt gcaatggatc gattcagtta actgatccgc ccacccgact gcccatctat
    45901 tgatccagaa caggtaatca gtatgacgaa tacttaaaat cgtcatactt atttccgcca
    45961 tctattttaa tccattgggg ttaccatgct ctccacactc cgccgcactc tatttgcgct
    46021 gctggcttgt gcgtctttta tcgtccatgc cgctgcacca gatgaaatca ccaccgcctg
    46081 gccggtgaat gtcgggccac taaacccgca cctttacacg cctaaccaga tgttcgccca
    46141 gagcatggtt tatgaaccat tggtgaaata tcaggcagac ggttcggtga tcccgtggct
    46201 ggcaaaaagc tggactcatt cagaagatgg taaaacctgg accttcaccc tgcgtgatga
    46261 cgtgaaattc tccaacggtg aaccgttcga tgccgaggcg gcggcagaaa acttccgcgc
    46321 agtgctcgat aaccgtcaac gtcacgcctg gctggagctg gcaaaccaga ttgttgatgt
    46381 taaagcactc aataaaacag agctgcaaat taccctgaaa agcgcctact atcctttcct
    46441 gcaagaactg gccctgcccc gtcctttccg ttttatcgct ccctcgcagt ttaaaaacca
    46501 tgaaaccatg aacggaatta aagcgccgat tggcaccgga ccgtggattt tgcaggaatc
    46561 gaaactgaat cagtacgatg tcttcgtccg taacgaaaac tactggggcg aaaagccagc
    46621 gattaaaaag atcaccttta acgtcatccc ggacccgact acccgcgcgg tggcgtttga
    46681 aactggcgat atcgacctgc tgtacggaaa cgaagggtta ttaccgctcg ataccttcgc
    46741 ccgctttagc cagaatccgg cttaccacac ccaactgtca cagccgctcg aaaccgtgat
    46801 gctggcgctc aataccgcca aagcccccac caacgagctg gcagtacgtg aagctcttaa
    46861 ttacgcggta aacaaaaaat cgctgattga taacgcgtta tatggcaccc agcaggtcgc
    46921 cgacaccctg tttgcccctt ctgtgcctta cgctaatctc ggcctgaaac cgcgccagta
    46981 cgatccgcag aaagcgaaag agttgctgga aaaagccggt tggacgctgc ctgcgggcaa
    47041 agacatccgc gagaaaaatg gtcagccgct gcgcattgaa ctgtcgttca tcggcaccga
    47101 tgcgttaagc aaatcgatgg cggaaatcat tcaggctgat atgcgccaga ttggcgcaga
    47161 tgtctcgctg attggcgaag aagagagcag tatctatgct cgtcagcgcg acggtcgttt
    47221 tggcatgatt ttccaccgca cctggggcgc gccatacgat ccacacgcct tcctgagttc
    47281 aatgcgcgta ccgtcgcacg ctgacttcca ggcacagcaa ggattagccg acaagccgct
    47341 gattgataaa gagatcggcg aagtgctggc gacccatgac gaaacgcaac gtcaggcgct
    47401 gtatcgcgac attctgaccc gtctgcatga cgaggcggtt tatctgccta tcagctacat
    47461 ctcgatgatg gtggtatcaa aaccggagct gggtaatatc ccctacgcgc cgatcgccac
    47521 cgaaattccg ttcgaacaga ttaaaccggt gaaaccttaa tgttgcgtta cgtattacgc
    47581 cgctttctgc tgctgatccc gatggtgctc gccgcctcgg tgatcatttt tctgatgctg
    47641 cgcctcggta ccggcgaccc ggcgctcgat tatctgcgtt tatctaacct gccgccgacg
    47701 ccggagatgc tggcctctac ccgcaccatg ctcgggctgg atcagccgct gtacgtccag
    47761 tacggtacct ggttgtggaa ggcgctgcat cttgactttg gtatctcatt cgccagccaa
    47821 cgcccggtac tggacgatat gctgaacttc ctgcccgcca cgctggaact tgcaggtgcg
    47881 gcgctggtat taattctgct cacttccgta ccgctcggta tctgggcggc gcgccaccgc
    47941 gatcgtttgc cggatttcgc cgtgcgcttt atcgcgtttc ttggcgtgtc gatgcccaac
    48001 ttctggctgg catttttgct agtgatggcg ttttcggtgt atctgcaatg gctacccgcg
    48061 atgggctacg gcagctggca gcacatcatt ttacctgcgg tttccatcgc ctttatgtcg
    48121 ctggcgatta acgcgcgact gctgcgcgcc agtatgctgg acgtcgccgg tcagcgtcac
    48181 gtcacctggg cgcggctacg cggcctgaat gacaaacaga ccgaacgccg ccatattctg
    48241 cgcaatgcct cgctgccgat gatcaccgcc gtggggatgc acattggcga attgattggc
    48301 gggacgatga ttatcgaaaa catctttgcc tggcctggcg tcgggcgcta tgcggtgtcg
    48361 gcgattttta accgtgacta tccggtgatt cagtgcttta cgctgatgat ggtggtggtt
    48421 tttgtggtct gtaatttgat tgtcgatttg ctcaacgccg cgctggaccc gcgcattcgt
    48481 cgtcatgaag gagcgcacgc gtgaactttt tcctctcttc ccgctggtcg gtacgcctgg
    48541 cgctgatcat tatcgccctg ctggcgctga ttgcgctcac cagccagtgg tggctgccgt
    48601 acgatccaca ggcaattgat ttgccgtcgt gcctgctttc gccggatgcg cagcactggc
    48661 tgggcaccga tcacttaggt cgcgatattt tctcgcggct gatggcagcg acccgcgtgt
    48721 cgctcggttc ggtaatggcc tgcctgctgc tggtgctgat attagggctg gttattggcg
    48781 gcagcgccgg gttgattggc gggcgcgttg atcaggccac catgcgcgtc gccgatatgt
    48841 ttatgacctt cccgacctcg attctgtcgt tctttatggt tggcgtgctc ggcaccgggc
    48901 tgaccaacgt aattatcgcc atcgccctgt cgcactgggc gtggtatgca cgcatggtgc
    48961 gcagcttggt gatttcacta cgccaacgcg agtttgtgct ggcgtcacgg ctttccggtg
    49021 cgggccatgt gcgggtgttt gtcgatcatc tggcaggcgc ggtgatccct tcgctgctgg
    49081 tgctggcaac gctggatatc ggccatatga tgctgcacgt cgcggggatg tctttccttg
    49141 gcctcggtgt gaccgcgccg accgccgaat ggggcgtgat gattaacgac gcgcgccagt
    49201 atatctggac ccagccgctg caaatgttct ggccggggct ggcgctgttt atcagcgtga
    49261 tggcctttaa cctggtgggt gacgcactgc gcgatcatct ggatcctcat ctggtgacgg
    49321 agcacgcaca ctaatgccgc aacagattga actacgtaat atcgcgctac aggccgcgca
    49381 gccgctggta cacggtgtat cgttaaccct gcaacgcggg cgcgtgctgg cgttagtcgg
    49441 cggtagcggc agcgggaaat cattaacctg cgccgcgacg ctgggcattt tgcccgctgg
    49501 cgttcgccag acggcggggg aaattttagc cgatggcaaa ccggtttcgc cttacgccct
    49561 gcgcggcatc aaaattgcca ccatcatgca gaacccgcgc agcgccttta atccactgca
    49621 caccatgcac acccacgcgc gggaaacctg cctggcgtta gggaaacccg ccgatgacgc
    49681 tacgcttacc gctgccatag aagcggtggg gctggaaaac gccgcgcgcg tgctgaagct
    49741 gtacccgttc gagatgagcg gcggcatgtt gcagcgcatg atgattgcga tggcggtgct
    49801 gtgtgaatca ccgtttatca tcgccgatga accgaccacc gacctcgacg tggtagcaca
    49861 ggcgcgcatc ctcgatctgc tggaaagcat tatgcaaaaa caagcgccgg gaatgctgct
    49921 ggtcacccat gatatgggcg ttgtggcgcg tctggcggat gacgtggcgg tgatgtcaca
    49981 aggtaaaatt gtcgaacagg gcgatgtaga aacgctgttt aacgccccca aacatacggt
    50041 gacgcgcagc ctggtttccg ctcatctcgc cctctacggt atggagctgg catcatgacc
    50101 ttacttaacg tctgcggcct ttcccatcac tatgcccacg gtggatttag cggaaaacat
    50161 caacatcagg cggtgctgaa taacgtttcc ctgaccctga aaagcggcga aaccgtcgcc
    50221 ctgctgggac gcagcggctg cggaaaaagc accctcgcgc ggctgctggt aggcttagag
    50281 tctccctcac aagggaatat tagctggcgc ggcgaatcgc tggcgaaact taatcgcgcc
    50341 caacgtaaag ccttccgccg tgatattcag atggtgtttc aggactccat cagtgccgtg
    50401 aatccgcgca aaaccgtgcg cgagatcctg cgtgaaccga tgcgccacct gctatcactg
    50461 aaaaaatccg aacaactggc gcgcgccagc gaaatgctga aggcggtcga tctcgatgac
    50521 agcgttctcg acaaacgccc accgcagtta agcggcggcc agctccagct cgtctgcctg
    50581 gcacgcgcgc tggcggtcga accgaaattg ctgattctgg atgaagccgt ttctaacctt
    50641 gatctcgtgt tacaggcggg cgtcattcgc ctgctgaaaa atctacaaca acagtttggc
    50701 actgcctgcc tgttcatcac tcatgactta cgcctggtgg aacgcttttg ccagcgagtg
    50761 atggtgatgg acaacggaca aatcgccgaa acccaggcgg tgggagataa attaaccttt
    50821 tcctctgacg ccggacgtgt gctacaaaac gcggtattac ccgcattccc cgtgcgccgt
    50881 cgcacctcag aaaaggttta acgccaatgc aacgagtcac catcacgctt gatgacgatt
    50941 tactggagac gctggacagc ctgagccagc gtcgtggtta taacaaccgt tccgaagcta
    51001 tccgcgatat tctgcgtagt gccctggcgc aggaagctac ccagcagcac ggcacacaag
    51061 gtttcgcggt gctgtcgtat gtgtatgaac acgaaaaacg cgacttagcc agccgtattg
    51121 tttccaccca gcatcatcac cacgacctct ccgtcgccac gctacatgtg cacatcaacc
    51181 acgacgactg tctggaaatc gccgtgttga aaggtgacat gggtgacgtg cagcattttg
    51241 ccgatgacgt tatcgcccag cgcggcgtgc ggcacgggca tttgcagtgc ctgccgaagg
    51301 aagattgagt tatttctaaa acaaggaaag aaaagatatt aactgaactt aatttataaa
    51361 attaagttca gttgcataga tagatcctgt aaaggcagat attctgcgta atgtacgtcg
    51421 ctaaaattta taaatgattc aaacgctcat tcagtgtttc aataatatag gtattaacag
    51481 agacttgttg ctgcgcagcc gcattattaa ggcgttcact cagcgattct ggatagcgta
    51541 acgtaaaggt cttaatcttt tctgtacgcg catacggctc aatacccgca gctttacaat
    51601 cctcaagata ctctcgcagg gaaagctctc cttctttttg cagcccctga atactgtcag
    51661 agacaaaatc acaataacct gacaagccca ggaatttgcc acgaaatgca ttcagttctg
    51721 gcacgtaggt aataacagct ggttgcccgg caatctccat agagtttggc gtcttcagtt
    51781 tgatcatggt tttaccccta tacttagtaa ccagtcacgc acgctttcta cagcgccttt
    51841 atcggtatct ggcgacggat gaggccgatg gaaacacgca acgctcatat ttagtatgaa
    51901 cttgcaacgc gaacctcttc cttccttaat ttctccgcct aatgctttaa ccagtgactc
    51961 tatatcagac cacttgatcc cttgaggaac aggtgtttta aatatttgat ccaatgtatt
    52021 tttttgtttc ttccgtaaag cctgtacctt ggttctcatg gcatgtcctt atgacttcac
    52081 tttatgacat cacagtatga agtcaagttt cccttcctta cgccattgtc ccaatcgtct
    52141 tcctgaatcg cagcagtgcg atggtgaaaa acgcgccgcc aattgccatc agcgtcagga
    52201 actgcggcca gacgatttcg aatccggcac cccggtagag gatggcctgc gcgaggctaa
    52261 caaagtgtgt cgtcggcatg gtcagcataa tgtcctgcac catctgcggc atactttcgc
    52321 gcggcgtgga tccaccggaa agcatttgca gcggcagcag caccagaatc accagcagcc
    52381 ccagttgcgg cattgaacgc gctatcgtcc ccataaaaat gccgattgac gtggtggcaa
    52441 acagactgag cgccacgccc agcataaaca gcgggatcga gccttcaatc ggtacgccca
    52501 gtacaccttt caccatcagc accagcgata atcccgatac caccagcacc accagcccca
    52561 tcgaccagat cttcgccatc atgatctcaa acggcgttat cggcatcacc agtaaatgtt
    52621 ccaccgtgcc gtgttcacgc tcgcggatca gcgccgatcc ggtcaataca atcgccagca
    52681 tggtaatgtt gttgatgatc gccatcaccc cgccaaacca cgcgggatca aggttcgggt
    52741 taaagcgcat ccgggtttcc agcgatacca acggttcgct gttatcacgg tagcgcgcga
    52801 caaagctgtt cacttcaccg ttgataatat tctggatata cccattgccg gtaaatgcct
    52861 ggctcatgcg cgtggcatcg acgttcacct gaatatccgg ctggcgtccg gcgaggacat
    52921 cacgctgaaa attaggcgga atatttatcg cgaaggtata gcgtccggcg tccagtccgg
    52981 catccatctc atcggcggtg atcatctccg gtggcaaaaa ccacggacga tagaagctgt
    53041 taacgatccg gttcgataac tgcgattgat ccatatcggc aatggcgatc ggcgcgaggt
    53101 tcaacgatcc tggcgtaacg gtcgctgacg aatacaccga caccgtaaac gagaagacaa
    53161 tcagcgtcag catcgcttta tcaccgagca gactgcgcaa ctctttgata cccagattaa
    53221 aaatattgcg taactggcgc atcatccctc ctgttttttc agcagcagga tgcttaagcc
    53281 catcaccagc gggatggcta tcagcaacgg gataaaaagt tgccacaaat cagtcagatc
    53341 cagcgctttc gagaacgttc cgcgggcgat agtcagaaaa tgactggtcg ggtaaacctc
    53401 gccgatccaa cgtccaggcc cttccagcga agctaccgga tcgatcatcc cggaaaactg
    53461 tgtcgccggg atcaatgtga taatcgccgt tccgaaaatg gcggcaatct ggcttttcat
    53521 aaaggtggag atcagcagcc ccattccagt ggcaatgatg atatacagca gcgccgccaa
    53581 ggtgagcgtc aggaaactgc ctttatgcgg tacgccaaac acaaacaccg acaggccgca
    53641 gagcaggaaa aagttcagca tccccagcgc gatgtacggc agctgtttgc caagcaaaaa
    53701 ttcgctacgg gtggtggggg tcacgtaaag gttgataatc gaccctagct ctttttcccg
    53761 caccacgcta agggcgctta gcattgacgg gatcatcatc agcagaagcg ggatcaccgc
    53821 cggaacaatc gctggcaggc tttttacgtc cgggttatag cgataacgcg tctcaatgtt
    53881 catcagcccg ctttggctgg cgggtgtcga ttgtcggctc gccacatcct gaagccaact
    53941 ctggtgcatt gcctgcacgt aaccttttac cgtttcggca cgactgggca tcgctccgtc
    54001 gatccagacg ccgagttcca caggcgtacc acgcgcgata tcgcgcccga aattgggcgg
    54061 gatctcaatg gccaccgtga tatcgcccgc acgcatccga cgatcaagct cgtcataact
    54121 ggtgagcggc ggctgttcga taaagtaacg ggaaccggag aggttgagtg tccacgcctg
    54181 gctactgacg gtctggtcgc ggtcgagcac cgcaaagcgc aggttttcca catccatact
    54241 gatgccgtaa cccattatca gcatcaggat caccgttccc atcagcgcca gcgtcgaacg
    54301 tactggatcg cgtcgcagtt ccagcgcttc gcggcggctg tagctaaaca gacggcgcag
    54361 gctaaatccc tgacgcggcg cgtgggtggt gtcgtgtacc acgggcggcg cttcggcttc
    54421 gttgctctgc cctgccgctt cctgcaaata ggcgataaat gcctcttcca gactggcggc
    54481 tccgcgtttc tcaaccagtt cctgcggtgt accgctggca agcacttttc cggcgtgcat
    54541 cagtgagatg cggtcgcaac gttccgcttc gttcataaag tgggtggaga tgaagatagt
    54601 cactttgtcc tggcgcgaga gatcgaccat caactgccag aacatatccc tcgccaccgg
    54661 atcgacacca gaagtaggct catcgaggat taacatctcc gggcgatgaa tcaccgccac
    54721 cgccagcgaa agccgctggc gaatgccgag cggcaatgac tccggcagaa catcttcaac
    54781 gtcgttgagc ttaaaacgct cgctcatttc agccactcgc gcgggaattt ccgcttccgg
    54841 gatgtgaaac aaacgggcat gtaactccag gttttgccgc acggtgagtt cgttatagag
    54901 cgaaaacgcc tgcgacatat agcccacacg acggcgggta tcgatatctt ttggatcaac
    54961 cggttgccca aatagccacg cctcaccttc gctggcgggc agcagtccgg taagcatttt
    55021 catggtggtg gatttaccgc agccgttcga accaaggaat ccaaaaatct ccccgcgtgg
    55081 aatgcggaaa ttgacgtgat caacggcaac gaaggaacca aaacgcatgg tcagatcgcg
    55141 cgcttcgatg gcaatctctg cgttttcagg ttgatacggt gggatcacta ccgcctgatg
    55201 cgcctggcgt tgcgcttgcg gtaacagatt aataaatgct tcttccagcg tggcgctttg
    55261 cgtttgctgc cgcagctctt cggcgctgcc ggttgccagc acttctccgg cattcatcgc
    55321 taccagccag tcgaagcgtt cggcctcttc catataggcg gtggcgacca gcacgctcat
    55381 attgctctgc cgctggcgaa tactgtcgat cagatcccag aactgggcgc gggagagcgg
    55441 gtcaaccccc gttgttggct catcaaggat caacagttcc gggtcgtgga ttaacgcgca
    55501 gcacagccca agtttttgct tcatcccgcc ggagagttta cctgccggac gatcgcgaaa
    55561 cggtgctaac ccggtgctgg tcagcagctc attaattcgc acttcccgct ccgctttatc
    55621 gtgaccgaac aggcgagcga aaaaatcgac attttcatac accgacaagg tgtggtagag
    55681 gtttttgccc agcccctgcg gcatccaggc gatgcgcggg cagacgtctc ggcgatgctt
    55741 cgggtcgtgc atatcgccac ccagcaccac cacattgccc tgctcaatga cgcgggcacc
    55801 ggaaatcagt gacaataaac tcgacttccc gacgccatcc ggaccaatca gcccgaccat
    55861 gcaacgggcc ggaatatcga gagtgatatt gttcagcgca acggtttttc cataatgctg
    55921 gctcacgccc gccagttgcg cgacaggcgg gacaggaacc agttccagat gcgtcattgc
    55981 ggcaacctca ccacgaggtc gtcaggccac ggaagttctt cattcacccg cacccacgct
    56041 acgcccggta aaccggtttt gacatattcc agatgctgct ggagtaattc cggtgggata
    56101 cgcgctttga cgcggaacat cagtttcagc cgttcatcgc tggtttcgac ggtttttggc
    56161 gtgaactggg cgacactggc gacaaaactg atggttgcag gaatacgcag atctggcgcg
    56221 gcatcgagga tcagccgggc ttcaccgccc agtttcagcg tgcccgcctg ttcggttggc
    56281 aggaagaagg tcatatagac gtcgctgaga tcgaccatat tcagcacccg accgcctgcc
    56341 gccagcactt cccctggctc ggcaacccga tactgcacgc gtccgtcacg cggggctttc
    56401 agttcactgt catcgatatc tgcggcaatg cgccgttcag tggcttgcgc tgcttcgacc
    56461 cgagtttgcg cctgaatgat attggtgcgt gctgcttcta tagccgcttt agaagccgaa
    56521 acctgggctt tcgctgattc cagcgcagct cgggcgctct cagcggcggc gcggtcgtca
    56581 tccagctgtt gcgcagaaat agcccctcgt tgggcgagcg agcgggaacg cgtatgacgt
    56641 ttggcaacag agtccagttc ggcctggcgt tgattgacca gcgactgtgc ggcacgagtt
    56701 tcactttgtc gttgctccag caaagcctgc gcggcggcaa cggcgctttg tgcctctttg
    56761 atttgcgcga tggcttccag tcgctgttcc tgcaacacgc gagtatccat cttcgccagc
    56821 acttcgcctt cgcgaacaaa ctggccttct ttcaccagaa tggtgtcgat acgcccggca
    56881 gttttgctgg cgatatccac ttccgtcgct tcaatgcgcc cattgctgac agcaaagcct
    56941 tccggcacac ccgccgggcg caacagccac cacgccacga cggcagccac cgccagtgcc
    57001 ccgacaaccc accacgccag atggcgctta ctcttatcca taatcgaccc gccataatcc
    57061 ctgtaagcaa acgacgcgtg ttacaactgc cgccgaacga cgttcagcgg attcttccat
    57121 ccgggataag aaggaaacga caaatacgac cagcgcgcag agcattgagc gccaacgggg
    57181 aaggcttagc gaaaacgggt agtaatgttg tcatcatcag cattcctggc cgtaaatgaa
    57241 acacgcatac tcataagtgt gatctataaa actggctgac aaaacggtat ccatcctgtt
    57301 ggatcgcgtt tcacgcctgt tcagttgcag gaacagagca ttccttttta cgttttatca
    57361 acagtctcaa ccagatagta acattaatat tcatgttgtc tatggttcag agctgtaaca
    57421 ttgtgcgcct tctaatcaaa ggcataagtc catttctgtt tttttcatgc tggtaagact
    57481 tccagggagt ggttatctct ggccctgcac cagggttaat ttggcaatta tgcatgctga
    57541 acattcttcc agcatgttta ctgtcacggg cgttaagacc taaaactgtc cccgtttcac
    57601 ctatgcgttc tctcccacca tttccgttat gtgcaacaac ttgatttata cctaacattt
    57661 gtgaacatgc taatagtgct tcaagtttag gtctgtaatc tgtcgaaaca gaaaaataac
    57721 gattaaagga tcgacaagag atagcccggc tagttttaaa ctttttgcca ttaattatag
    57781 catgatgctt tttattaata atttccgcta attcgaccgg atcgagagca ttggtcattt
    57841 catcaataga gacagttatc ggtcctataa tgtaacgttt tcgctgatca tcaaaagtta
    57901 tcccatgatg atttgccatt attttgtatt tggggtcata gaaacataca ggataatctt
    57961 ttatcccgtc gtaggtatcc tcatcaagtg gtttcatgcg cgtgaagttt tgcatatagt
    58021 taccattgca attcgtttca tgatttccag ctaatacatg aacattttta ttgatactca
    58081 ttatgcgttt taacaaatcg atgataaatt gaacgccaca gatattacta aatcgatcgc
    58141 cggtttggtc gccaagaaac gtacatggcg tgactgcgtc tttattaata accaaataag
    58201 gctgaagact ttctaaaaca ttatctcttc tgttcagtat actgattatt gtattaacat
    58261 tagtgtcaag ttgctcatta aagttctccc ttagcaatac gttgagaact tcatcttcta
    58321 tattaagcaa ctggctaagg aaattttctc cttcagccat gattcccaaa tgtcctgttg
    58381 caaaaagcgc gtacaaaact gcaccgactg agctatcggt atcgccaaaa taggtcggcc
    58441 tggtacttgt gggatcaaca ggtgaacaat tggagaaata ggttctgtca gcgaactggg
    58501 gttttataat tgtttcgaat ttaatatcaa atacattgta ttgcgatatt tcccagttcc
    58561 agatgggttt gtacgactca gtaactgcaa catcattagc attatcattt actgtcatat
    58621 tattaagtaa ataaacaatg ctgcaccaat taatatcggt attatcaaga gggcaaccgt
    58681 gatttttact attaattgtc acaataaact gataacagtt attcctacgt agaatatgag
    58741 cgcgcatgcc cgatagcata ctatattttt ctgaatgatt cgtttgtatg gtaaatgaaa
    58801 ttttgtcatc ttgaatagat tgttggaaac gttctatttg ccaatcatca attttttcca
    58861 caacttcacc gagcattgtc ctggcaatac ttgatatatc atcacctata ccatttgcaa
    58921 tatacccaaa cagttcttta gtgaagttgg taacatgctc ttgcaccata tcatttgttg
    58981 cgattgtcgt tgtactgcca ttagtgcctg ccacagttcc aattttcatc ttttacctca
    59041 tattattcac cgcgaggaat acatagttaa cattttatct tatgacatac acctcatatt
    59101 cgatgggaaa ataattatat tttgcacaaa tcattcagcc ttaaagcata atatgaaatt
    59161 tcttatctca tagcagagaa agtccctaaa gattgtagag gcgtcatcag tacatacctc
    59221 tacaatcgat taagaaaacc tatgaaatac tggcaaaatt aaaaagagat accacagcac
    59281 tcatgaagtt ttattgaggt tgaaaacaac taagccctgg gacacctttt ttatcaccac
    59341 gttcgttagg tgctatcttc caagaattta gccccatggt ataattatca attttttgat
    59401 gctggtctac ccctaatcca taatggccaa atttctgttt agtttttttg agcgtctgtc
    59461 cacatttgaa gatatcttca ggttttggtc tgaaagcagt agtgttatta aaataatttt
    59521 catatacagg tgtgggtgct atagcattgc ttctaattag atggagaaga tgatcatcca
    59581 tgtgttgttt atgtttttta ttaaatatat ttgctaattc ctcaggatca attgggtctt
    59641 tcatctgatt aaatggaacc tgcaaagcac cgagacaata acattgactt tcttcatttc
    59701 ttattattcc gtgatgactg gttaaaactt tagtttctga gttataatta caaacatcga
    59761 gcgttttaat taagttatac gtatcaccgg cagagagttt ataattttct agtcgtgcag
    59821 tatagttacc attaaaattg atttcatgat ttccagccag aacgatgaca tttttattaa
    59881 tacggctatc tttattaccc tccatatttc gcatagaatt tagcaaagta aggatatatt
    59941 tatcaccaaa aatagtacta aatctatcgc ctgtatgatc cccaaggggg atacaagggg
    60001 ttatcgcatc gtcctttaaa aaaacatgtt gatgcaactc attcaatatc atgtctctcg
    60061 tatttaataa attgtagagc aatttattat ttttatgtat gtatgcagat gaagccatgt
    60121 cttcgtgttt taataattca cataataaac tccaaccctt ttccctgatg cctatatgcc
    60181 ctgaaacgag caatgaataa agtacagcgc ctactcttcc atctgtatcg ccaaaataag
    60241 ccgtgttggc attttgtggg ttaatcacac cacattgttt ataaaagtct attttagaaa
    60301 acacagattt aacatagcta tcaaacttat ccttaggaaa cgtctcttta gagacatccc
    60361 aacgccagtc agctttaaaa ttaccgctaa gaagagtaaa gaaattttca ggtatgatag
    60421 aaagcccatt aactttataa attatgtctg atttatctcc ggagaaatta ataaaataat
    60481 aaccatcctt acactcaatc ttaactgtaa tgggagacga taattttatc acatggttat
    60541 tgtctggtat aattgtaaag ataaacgtgt tagtagtatt atcggcaaaa ctattatatt
    60601 cttgaatatc tatagatggc tcactattgt aaaaccatat tttctccatc tgaagactat
    60661 gttcatttac cctccagtga ccatgttgca ggaaatagat gatatgcatg ccaaaatcgt
    60721 tatctatttg cgaatcatta tccagtttat caatttcaac tatctttgaa cgatgtgttt
    60781 tatcaacctt gctatggtgt tgatgattat taactttatc tgaatgctta tctttatggt
    60841 ttgaagggat gtacttaaat atattgtcaa ttgctttcat aaataacccg cgatattttt
    60901 gacctgttag taaaaaataa atgatacatg tctgaaagac atatttacga cgatataaaa
    60961 caataatgct tatatgttcg ctgattttaa gcaccgagac tatccggtct tttactaaat
    61021 aatggaaaac aatccatccc tggaagacaa ttgtaaaaaa aacaacagaa taagaattaa
    61081 tcttaggata aatatttatt tatcatggcc ttttgaccgc taaaaaatgg cactcggcac
    61141 agtaaacccc gctatagtta tgctatacat ttgtctttaa tgatatctcg tcgttatatt
    61201 aacaggctga aattatcatt aatgcacatt tcaatattag cagggatacc tgaggagttt
    61261 aaagtgaaca tatatatcgg gtagcttttc aaattaatcc ctttgattat aggcttaatt
    61321 tgcatcgcgt taggtggctt tgtgctggaa agttcagggc aacgtcacgg gtcatgtgct
    61381 gatttctctg gcggccatat gcctggcatt attcactacc gcatttatta tcatttcgca
    61441 gctcacgcgc ggcgttaata cgttttacaa tacattgttc cccattattg gctatgcggg
    61501 gtcaattatc accatgatat ggggttgggc actgttagca ggtaatgatg tgatggcaga
    61561 cgagtttgtt gccggccatg ttatttttgg cgttggtatg attgccgcat gtgtatcgac
    61621 ggtggcagcg tcgtccggtc actttctgct cattcccaaa aatgcagcgg ggagcaagag
    61681 cgacggaaca ccggtacagg cttattcttc attaatcggt aattgcctca ttgccgttcc
    61741 cgttttactc accctgctcg gtttcatttg gtctattacg ctgttacata gtgccgacat
    61801 aactccgcat tatgtcgcct tgggttaacc gcaatctgtg cctgtctaat tggcctagtg
    61861 gccacaatcg tccatcaaac acgtaatacg ttctcatcca aagagcactg gctgtggtgt
    61921 tattgggtta tttttctcgg ctcagtcacg gtgctgcagg ggatatacgt cttagtcagt
    61981 tccgatgcaa gcgcccgact ggctcccggc attattctca tttgcctcgg aatgatctgt
    62041 tacagcatat tctcaaaagt ctggctactg gcactggtct ggagacgtac ctgttcgtta
    62101 gccaacagaa taccgatgat accggtattt acctgtctgt tttgcttgtt tttgtcatca
    62161 ttcctcgcgg aaatcgcaca aactgagctg gactacttta ttccatctcg ggttctggtt
    62221 ggattaagcg cagtatgttt tacattgttc tctattgttt caatattaga ggccggttct
    62281 gcgaaaaaaa attaaaattt acataatccc agcgttattc atatcttaac gctggttttt
    62341 tacgaactta gtgaaataat gtagaatata aaattataat tttatattgg agcacatgtg
    62401 ttaatgatca accccgggat actaaaaata tagacattat taaacttgct tagcctcgca
    62461 ttgcctttac ctgttaaaga tgcatcgaga aaattgagtc tgaagttctc atcctttatc
    62521 tgattttcca gtcccgaaac tttggcctta gcattacctg agatccgtcc gttactatct
    62581 attttgtagg taaaaaaata cgaatgggaa aatttcctcg gtgtatcttc atcagttaag
    62641 ccaacaatcg taaaactacc gctaccatct ttttttaaaa tgactgaaag gttagcagag
    62701 tagtataaag aatgatctgt tttagcattt cttgttaacg tcgacgtaca ttcaagagac
    62761 gttgtgctac catcacgagc cttccagaga gcaaaaaaaa acacctgtag caaaaataca
    62821 ttttcttatt agtcatataa tagcacctct gaacaaattg cataagtttc gccattagag
    62881 gattttatac atcttgataa ataccgctgc ccttccccaa gcaaataatg catactattt
    62941 acatcatata taaaatttgc acctggcaca cagggtaaat gctttttcga aatttcagcg
    63001 gcctctattt gagatttagc gaggccaatc tcgttaagat tcgctgaaag atataaatca
    63061 caggtgtcaa tcttacctga tttaaccaat ggataatccg ttatactgta ggtatcttta
    63121 aaagccacgg caaacatttc aataataaca attactacca caagcgaaat caaaatgcga
    63181 aaaagaggaa tactcctttt gcgctctact ttttcaacaa catcttcttg taaatgagaa
    63241 tcatcataag attgtttact tattgttatg ttttcactga tgtaaaagcc tactttgggt
    63301 atcgtaagaa tcacattttc ttcaacacca atatttttaa aacttttgcg taacagggat
    63361 atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa atctctccaa aacgctatca
    63421 atgattgagt tcctgtcaac cgctccacca ttactttcca gtaacactgc caaaatacac
    63481 cccgccgtag ctggcaaatt tatctcatcc tgggtattta cattcgagag cgagcatttg
    63541 ttcggatcaa aaataatcgc gtcattgata atgaaaaaac atctccgctc catgcaggca
    63601 acaacacaaa ataagatttt gtcattttag cctattcctg gtggaactga cacaagaaac
    63661 taaaatacaa atataaccct gaaaaagtca tataaaactt atatccacag aataaaaaaa
    63721 caaaacatgt cagttttatc aatataaagt atcgcaaggc gaaaagtgat gcaagaatac
    63781 tcctaagtaa atgaaagcgc accacatgaa attatcaaca catgtaattt catcattagg
    63841 tgctatcgaa aataactcag gagctactta agatgaataa tgtcttactc atgagacccg
    63901 atgactcaga atttatgata cctgatgact ggtcagggct aaacgcaaaa ggcattacat
    63961 acgaacaaga taatagagtg acagagtctt ttctgccttt taaatctttt tattgttcac
    64021 cggtgtgcac gacagttgaa ctcatctcac aaaataattg ttttgctatg ggtatccgct
    64081 accttctgga aaagaaaaga gacttaaggc taaatcactt taaagccttc tctcataaat
    64141 gcgagacaaa acctgacgtg ttaattatga ccgggtgcac cgtcgctgat acttatagta
    64201 tattctcacg cattgttgaa gaaaatataa tgaaaacgat gacacctatc gtcatcatta
    64261 ctgggcatca ctcagaagat ctttgttctc tggcatcgaa actgggagga gttaaaataa
    64321 tatcaccgac agaaaagatt gcaaaaatag aaaacgctat caatgatgct tttaacagca
    64381 agcctaaaaa aataagattt ttaacagata tactctctct tcaacaaatt gagaccatga
    64441 aaatggttgc gcagggtaga tccgttaatg agatttcagc cgcaatgaat attagcatca
    64501 agaccgtgta tgcacataaa gttcgtgcta tcaataagct gggaattaag aaaaaagttt
    64561 atgaagcgct tttttataaa gcgctaattc agttcaactg atgtaaaaat caccctcgct
    64621 taataaataa tatattctat gtcttttttt ataagttaaa aaaggcggga tatcaaatcc
    64681 cgccatgaaa ttaactaatc atctgcatta ttctgcgtag atataagcct gggtacggat
    64741 ggtgtaagag tctggctcaa caacctgttc tttcatatgg gaaacgacgc gataggcacc
    64801 acctgttcca gtatttttca gcatcatata acgcttaccg ccaatctctt cggaggcagc
    64861 tgaaggagga tcctgctcaa gcgttgtcag ggaaactttc agtttatggt ccgggttatt
    64921 gtcaccagaa attactgcat ccgctgaagt actgctctcc gcataaatat catccgggaa
    64981 agttacagca tagtcgtggc tctgcccatc agatgcgcca acaatacctg ttgccaaagg
    65041 cgtccatgca gttaacgtac cgctatatac ccctttcact ggttgaatgg tattattaac
    65101 aacgataggc ttggtcaaag taagattagc tgacgcttca gtaccctggc ttactaaagt
    65161 tcccgcctga gaagcaaaag ctgtccccgc cagcatgatt gctaacaatg ttttcttcat
    65221 tatattttct ccatgatttt catttacact gcaaaatgca gtttctttct ttgcatttac
    65281 gtgctatcgc gaaaatgctt ccaccaaata atggaaacat cttcacaaaa ataaattatg
    65341 agtttcttaa tttagaaact ataaacatgt cgccgctatc tggtataacc ctgatgcact
    65401 tttcttcgca ggaagttggt attttgcgat acaggattga tctttagaat aaccccattt
    65461 cacattaaac tcgccttccg cactcaagcc agaaacataa gcaataccat cttcacctac
    65521 aatgccggcc atattttcag tgtctttgct ataactaatc acagcgccga aggggacggg
    65581 tttaccctct ttctgtttta aggttactaa tcctttatcc ccgctcttca ctgccaatgt
    65641 cgcaggcaca acggctccag ctgtcggtac aaccaattga ctagtttgtg gtaagtcgta
    65701 attcttacct atcgtttttt catccagaat aacatcgtct cttcggtatg gctgcactgg
    65761 cagtattgca tacccacgaa aatcagtact cagtcccggc cagccattaa cgttaacccc
    65821 cgttgctccg ggtgcctcta taagggctaa tgtcccatcc acattttgac caaatgtaag
    65881 tccatccctt gtaataagta tcccaccgct taaatttgca cctagctgtt tcatgttttc
    65941 gctggagctg taatggctac taaactgacc atatgatcca tttttgccaa cttgcacaat
    66001 gttcatcgtg ctcttcggtt gtgactgatg agcgttagtt atatttgtcg ctacatccca
    66061 ccatacacca ccactattct gacctgataa tgaaacctga tagttattac cctgctctcc
    66121 cgaacgactc atctgtgaag acgcataaat ttgattttcg tcacccagcg ataatggcac
    66181 tgaaataccc acgcttaatt tctgatctac tttccattta tgcgatgtca gcaagtcgtt
    66241 ttttgcccag ttaatggaga atgttatgcc tctccatacc gtactataac tcaggctggt
    66301 agaccgcgtg tttcctgtgc cccgggaaga attatacctc gtggtattga agttcaaatt
    66361 cccgatatta ccaaggggct gggataagga tatcgtagag ccatcgtgac tattcttatc
    66421 tctttttctg ttccgttcat cctgtgcact tactgcttca gtcagtgtgg cataacctga
    66481 tgcataacgg ttcgtggtta tagac
//
LOCUS       DAJTYI010000006        65122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYI010000006 DAJTYI010000000
VERSION     DAJTYI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..117)
                     /locus_tag="OO564_000404"
     CDS             complement(<1..117)
                     /locus_tag="OO564_000404"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="catalyzes the formation of
                     2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine
                     diphosphate from
                     2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine and
                     ATP; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6651660.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSS"
     gene            complement(114..1532)
                     /gene="pcnB"
                     /locus_tag="OO564_000405"
     CDS             complement(114..1532)
                     /gene="pcnB"
                     /locus_tag="OO564_000405"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6651661.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1571..2497)
                     /gene="gluQRS"
                     /locus_tag="OO564_000406"
     CDS             complement(1571..2497)
                     /gene="gluQRS"
                     /locus_tag="OO564_000406"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6651662.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2534..2989)
                     /gene="dksA"
                     /locus_tag="OO564_000407"
     CDS             complement(2534..2989)
                     /gene="dksA"
                     /locus_tag="OO564_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6651663.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3167..3871)
                     /gene="sfsA"
                     /locus_tag="OO564_000408"
     CDS             complement(3167..3871)
                     /gene="sfsA"
                     /locus_tag="OO564_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6651664.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3886..4416)
                     /gene="thpR"
                     /locus_tag="OO564_000409"
     CDS             complement(3886..4416)
                     /gene="thpR"
                     /locus_tag="OO564_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6651665.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4490..6919
                     /gene="hrpB"
                     /locus_tag="OO564_000410"
     CDS             4490..6919
                     /gene="hrpB"
                     /locus_tag="OO564_000410"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6651666.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7121..9655
                     /gene="mrcB"
                     /locus_tag="OO564_000411"
     CDS             7121..9655
                     /gene="mrcB"
                     /locus_tag="OO564_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6651667.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9875..12118
                     /gene="fhuA"
                     /locus_tag="OO564_000412"
     CDS             9875..12118
                     /gene="fhuA"
                     /locus_tag="OO564_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6651668.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12169..12966
                     /gene="fhuC"
                     /locus_tag="OO564_000413"
     CDS             12169..12966
                     /gene="fhuC"
                     /locus_tag="OO564_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6651669.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            12966..13856
                     /gene="fhuD"
                     /locus_tag="OO564_000414"
     CDS             12966..13856
                     /gene="fhuD"
                     /locus_tag="OO564_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6651670.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            13853..15835
                     /gene="fhuB"
                     /locus_tag="OO564_000415"
     CDS             13853..15835
                     /gene="fhuB"
                     /locus_tag="OO564_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6651671.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15870..17150)
                     /gene="hemL"
                     /locus_tag="OO564_000416"
     CDS             complement(15870..17150)
                     /gene="hemL"
                     /locus_tag="OO564_000416"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6651672.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17375..18796
                     /gene="clcA"
                     /locus_tag="OO564_000417"
     CDS             17375..18796
                     /gene="clcA"
                     /locus_tag="OO564_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6651673.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18820..18885
                     /locus_tag="OO564_000418"
     CDS             18820..18885
                     /locus_tag="OO564_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651674.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            18878..19222
                     /gene="erpA"
                     /locus_tag="OO564_000419"
     CDS             18878..19222
                     /gene="erpA"
                     /locus_tag="OO564_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6651675.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19269..19892)
                     /locus_tag="OO564_000420"
     CDS             complement(19269..19892)
                     /locus_tag="OO564_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6651676.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(19930..20730)
                     /gene="btuF"
                     /locus_tag="OO564_000421"
     CDS             complement(19930..20730)
                     /gene="btuF"
                     /locus_tag="OO564_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6651677.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20723..21421)
                     /gene="mtnN"
                     /locus_tag="OO564_000422"
     CDS             complement(20723..21421)
                     /gene="mtnN"
                     /locus_tag="OO564_000422"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6651678.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21505..23022
                     /gene="dgt"
                     /locus_tag="OO564_000423"
     CDS             21505..23022
                     /gene="dgt"
                     /locus_tag="OO564_000423"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6651679.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23152..24576
                     /gene="degP"
                     /locus_tag="OO564_000424"
     CDS             23152..24576
                     /gene="degP"
                     /locus_tag="OO564_000424"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6651680.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24731..25887
                     /gene="cdaR"
                     /locus_tag="OO564_000425"
                     /pseudo
     CDS             24731..25887
                     /gene="cdaR"
                     /locus_tag="OO564_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(25976..26362)
                     /locus_tag="OO564_000426"
     CDS             complement(25976..26362)
                     /locus_tag="OO564_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6651681.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26676..27500)
                     /gene="dapD"
                     /locus_tag="OO564_000427"
     CDS             complement(26676..27500)
                     /gene="dapD"
                     /locus_tag="OO564_000427"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6651682.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27531..30203)
                     /gene="glnD"
                     /locus_tag="OO564_000428"
     CDS             complement(27531..30203)
                     /gene="glnD"
                     /locus_tag="OO564_000428"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6651683.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30265..31059)
                     /gene="map"
                     /locus_tag="OO564_000429"
     CDS             complement(30265..31059)
                     /gene="map"
                     /locus_tag="OO564_000429"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6651684.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31427..32152
                     /gene="rpsB"
                     /locus_tag="OO564_000430"
     CDS             31427..32152
                     /gene="rpsB"
                     /locus_tag="OO564_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6651685.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32410..33261
                     /gene="tsf"
                     /locus_tag="OO564_000431"
     CDS             32410..33261
                     /gene="tsf"
                     /locus_tag="OO564_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6651686.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33408..34133
                     /gene="pyrH"
                     /locus_tag="OO564_000432"
     CDS             33408..34133
                     /gene="pyrH"
                     /locus_tag="OO564_000432"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6651687.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34425..34982
                     /gene="frr"
                     /locus_tag="OO564_000433"
     CDS             34425..34982
                     /gene="frr"
                     /locus_tag="OO564_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6651688.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35074..36270
                     /gene="ispC"
                     /locus_tag="OO564_000434"
     CDS             35074..36270
                     /gene="ispC"
                     /locus_tag="OO564_000434"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6651689.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36459..37217
                     /gene="ispU"
                     /locus_tag="OO564_000435"
                     /gene_synonym="uppS"
     CDS             36459..37217
                     /gene="ispU"
                     /locus_tag="OO564_000435"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6651690.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37230..38087
                     /gene="cdsA"
                     /locus_tag="OO564_000436"
     CDS             37230..38087
                     /gene="cdsA"
                     /locus_tag="OO564_000436"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6651691.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38099..39451
                     /gene="rseP"
                     /locus_tag="OO564_000437"
     CDS             38099..39451
                     /gene="rseP"
                     /locus_tag="OO564_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6651692.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39481..41913
                     /gene="bamA"
                     /locus_tag="OO564_000438"
     CDS             39481..41913
                     /gene="bamA"
                     /locus_tag="OO564_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6651693.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42035..42520
                     /gene="skp"
                     /locus_tag="OO564_000439"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42035..42520
                     /gene="skp"
                     /locus_tag="OO564_000439"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6651694.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42524..43549
                     /gene="lpxD"
                     /locus_tag="OO564_000440"
     CDS             42524..43549
                     /gene="lpxD"
                     /locus_tag="OO564_000440"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6651695.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43654..44109
                     /gene="fabZ"
                     /locus_tag="OO564_000441"
     CDS             43654..44109
                     /gene="fabZ"
                     /locus_tag="OO564_000441"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6651696.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44113..44901
                     /gene="lpxA"
                     /locus_tag="OO564_000442"
     CDS             44113..44901
                     /gene="lpxA"
                     /locus_tag="OO564_000442"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6651697.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44901..46049
                     /gene="lpxB"
                     /locus_tag="OO564_000443"
     CDS             44901..46049
                     /gene="lpxB"
                     /locus_tag="OO564_000443"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6651698.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46046..46642
                     /gene="rnhB"
                     /locus_tag="OO564_000444"
     CDS             46046..46642
                     /gene="rnhB"
                     /locus_tag="OO564_000444"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6651699.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46679..50161
                     /gene="dnaE"
                     /locus_tag="OO564_000445"
     CDS             46679..50161
                     /gene="dnaE"
                     /locus_tag="OO564_000445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6651700.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50174..51133
                     /gene="accA"
                     /locus_tag="OO564_000446"
     CDS             50174..51133
                     /gene="accA"
                     /locus_tag="OO564_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6651701.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDFVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51232..53373
                     /gene="ldcC"
                     /locus_tag="OO564_000447"
     CDS             51232..53373
                     /gene="ldcC"
                     /locus_tag="OO564_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6651702.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53430..53819
                     /locus_tag="OO564_000448"
     CDS             53430..53819
                     /locus_tag="OO564_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6651703.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53884..55182
                     /gene="tilS"
                     /locus_tag="OO564_000449"
     CDS             53884..55182
                     /gene="tilS"
                     /locus_tag="OO564_000449"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6651704.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55231..55491)
                     /gene="rof"
                     /locus_tag="OO564_000450"
     CDS             complement(55231..55491)
                     /gene="rof"
                     /locus_tag="OO564_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6651705.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55478..55678)
                     /locus_tag="OO564_000451"
     CDS             complement(55478..55678)
                     /locus_tag="OO564_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6651706.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55844..56377
                     /locus_tag="OO564_000452"
     CDS             55844..56377
                     /locus_tag="OO564_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6651707.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56386..56808
                     /gene="arfB"
                     /locus_tag="OO564_000453"
     CDS             56386..56808
                     /gene="arfB"
                     /locus_tag="OO564_000453"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6651708.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56822..57532
                     /gene="nlpE"
                     /locus_tag="OO564_000454"
                     /gene_synonym="cutF"
     CDS             56822..57532
                     /gene="nlpE"
                     /locus_tag="OO564_000454"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6651709.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57687..58511)
                     /locus_tag="OO564_000455"
     CDS             complement(57687..58511)
                     /locus_tag="OO564_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6651710.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58564..60282)
                     /gene="proS"
                     /locus_tag="OO564_000456"
     CDS             complement(58564..60282)
                     /gene="proS"
                     /locus_tag="OO564_000456"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6651711.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60393..61100)
                     /gene="tsaA"
                     /locus_tag="OO564_000457"
     CDS             complement(60393..61100)
                     /gene="tsaA"
                     /locus_tag="OO564_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6651712.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61097..61501)
                     /gene="rcsF"
                     /locus_tag="OO564_000458"
     CDS             complement(61097..61501)
                     /gene="rcsF"
                     /locus_tag="OO564_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6651713.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61619..62434)
                     /gene="metQ"
                     /locus_tag="OO564_000459"
     CDS             complement(61619..62434)
                     /gene="metQ"
                     /locus_tag="OO564_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6651714.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62474..63127)
                     /gene="metI"
                     /locus_tag="OO564_000460"
     CDS             complement(62474..63127)
                     /gene="metI"
                     /locus_tag="OO564_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6651715.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63120..64151)
                     /gene="metN"
                     /locus_tag="OO564_000461"
     CDS             complement(63120..64151)
                     /gene="metN"
                     /locus_tag="OO564_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6651716.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64339..64914
                     /gene="gmhB"
                     /locus_tag="OO564_000462"
     CDS             64339..64914
                     /gene="gmhB"
                     /locus_tag="OO564_000462"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6651717.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15376 a        16151 c        17968 g        15627 t
ORIGIN      
        1 cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca gggcagcatt
       61 gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca ctgtcatgtg
      121 gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg cggtgacggt
      181 tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac ctggaactca
      241 ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc tcgcaaggcc
      301 aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc gcgtttaccc
      361 tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt cgtcagacgt
      421 ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc cagcgcgaaa
      481 gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc cagcagtggg
      541 taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt atggatacgc
      601 gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc gccattttcc
      661 gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc acacaacagc
      721 ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc aaacaggcgt
      781 gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc ggtttccggg
      841 ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg taccggatct
      901 tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt cagatccttc
      961 ataccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag gctgttgata
     1021 gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc gcgcagcaac
     1081 atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc gtggtgtcca
     1141 cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag acggaaacgg
     1201 cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt ggcgttagtg
     1261 gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc gccgccaacc
     1321 agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc attttcactg
     1381 atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg tggacgggcg
     1441 actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa attagcgact
     1501 cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa aaatagcggc
     1561 taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc gactcgggca
     1621 cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg ctgaaatcct
     1681 gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt accgggcgtg
     1741 gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg ccttgtggat
     1801 taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc tggtacagcg
     1861 agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct gtaacgccct
     1921 ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca cggcgatgaa
     1981 tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc agcaggtcag
     2041 taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct ggtccatgat
     2101 gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc gcacgcgtac
     2161 aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca cgataggcgt
     2221 cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa tgttccagct
     2281 ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata tcttctatgc
     2341 gtaccagcca gcgaccttgc cgggcgcgac cctgcaaata gctgccgagc gcggcgatca
     2401 gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata tactgtgtgt
     2461 ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg taaacgtgat
     2521 ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc gttttgcagt
     2581 cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt tcaacaccgc
     2641 aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc tcgatctttt
     2701 tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct tcttcctggg
     2761 ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt gtaacggtgc
     2821 gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg aagtgcgcca
     2881 gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc accccagcga
     2941 tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg ttgcttctcc
     3001 ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat aaatagcaga
     3061 tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga ggaaaagcgt
     3121 attcgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac aatgtaaccg
     3181 gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa gccagaattt
     3241 ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg atgtggcgcg
     3301 cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc gcacgctggc
     3361 cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca gtgaccgcat
     3421 cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca atatagcagt
     3481 ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag ccgtatttta
     3541 cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta aggatagcct
     3601 ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg ctctggcttt
     3661 gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc gaataccaga
     3721 cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag tgtagcgtta
     3781 attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc tgaattagcg
     3841 tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt gcgttagcgc
     3901 ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg cgtaaagggt
     3961 gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca ccgcctcgct
     4021 ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa aacaaccgct
     4081 gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg gaggctgacg
     4141 catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa gcgtgagtgt
     4201 gaaacctggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt tctctgcgct
     4261 cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg gacgtcccgc
     4321 ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcgggttt ctgcaggtaa
     4381 gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt tatgaattac
     4441 cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg tgtcgtcgtt
     4501 gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc cgcaggtatt
     4561 attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac tgctggcgca
     4621 tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg cgcgtaacgt
     4681 cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg gctaccggat
     4741 gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtggttaccg aaggcgtgct
     4801 gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga tccttgatga
     4861 gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg atgtgcaaca
     4921 aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca atgaccgctt
     4981 gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt ttccggttga
     5041 acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg cggtagccac
     5101 cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg gcgtcggaga
     5161 aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg tgctctgccc
     5221 gctgtatggc gcgttgtcac tgaacgatca gcgaaaagcg atcctcccgg caccgcaagg
     5281 gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca tcgaaggtat
     5341 tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc gcacggggct
     5401 tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg ccgggcgcgc
     5461 cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag cagaacgcgc
     5521 cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc tgatggaatt
     5581 actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac cgccaacggt
     5641 gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg gtgaacggct
     5701 tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag cggcaatgct
     5761 ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg ccattctcga
     5821 agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca atcaaccagc
     5881 ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg gtgaggcaga
     5941 cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg ctcatcgccg
     6001 tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg atgccgacga
     6061 cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca gcgcctcgcc
     6121 ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac aacgctgtcc
     6181 gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta cgctgaaagc
     6241 ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg cgaaaccgtc
     6301 agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt taagcgtgct
     6361 caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg caaagtggtt
     6421 gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc tggaaacgtg
     6481 gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac tcgacattta
     6541 tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata gtgaattgcc
     6601 tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg aagataaccc
     6661 gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc cgacgatcgc
     6721 ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc cgctgcaaat
     6781 cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa aggagatgaa
     6841 agggcgttat cccaaacacg tctggccgaa cgacccggca aacacggcac cgacgcgacg
     6901 gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag ggccggggtg
     6961 agggcatcag cgcgcacgtt caccctcacc ctaaccctaa ccctctccct caagggagag
     7021 gggaccgatc gagcacaaat tttgagagat atcttcttct gtcttgtaac agaagaacag
     7081 aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga atgaccgcga
     7141 gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa gccgtcgtcg
     7201 ttacgaagat gacgatgatt acgacgatta tgatgactat gaggatgaag aaccgatgcc
     7261 gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg
     7321 gctactgcta aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct
     7381 cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg caactgcccg cggcagttta
     7441 tggccgaatg gtcaaccttg aaccagatat gaccatcagc aagaacgaaa tggtgaagct
     7501 gctggaggca acacagtatc gtcaggtgtc gaaaatgacc cgtcctggcg aatttaccgt
     7561 gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca gtaaagaagg
     7621 gcaggtgcgt gcgcgtctga cctttgatgg tgatcatctg gcggccatcg tcaatatgga
     7681 gaacaaccgt cagttcggtt tcttccgtct tgatccgcgt ctgatcacca tgatctcctc
     7741 gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt ttccctgatt tgctggtgga
     7801 tactttgctg gcgacagaag accgtcattt ttacgagcat gatggaatca gtctctactc
     7861 cattgggcgt gcggtgctgg caaacctgac cgcaggtcgc acggtgcagg gtgcgagtac
     7921 gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgttctt actggcgtaa
     7981 agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct
     8041 tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt
     8101 cccgctggca agcttgtatt actttggtcg cccggtagaa gagctaagcc tcgaccagca
     8161 ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac aacccgtggc gtaacccgaa
     8221 actggcgctg gagcgacgta atctggtgct gcgtctgctg caacagcaac agattattga
     8281 tcaagaactc tatgacatgt tgagtgcccg tccgctgggg gttcagccgc gtggtggggt
     8341 gatctctcct cagccagcct ttatgcagtt ggtgcgtcag gagttgcagg caaaactggg
     8401 cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca
     8461 ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac agcgtaagtt
     8521 gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat
     8581 ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcgtcgttc
     8641 gattggttcc cttgcaaaac cagcgactta tctgacggct ttaagccagc cgaaaatcta
     8701 tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg cgtcagccga atggccaggt
     8761 ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtgga
     8821 tgcgttgaca cgttcgatga acgtgccgac ggtaaatctg gggatggcgc tggggctgcc
     8881 tgcggttacg gagacctgga tcaaactggg cgtaccgaaa gatcagttgc atccggttcc
     8941 ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cattccagac
     9001 catcgccagc ggtggtaacc gtgcaccgct ttctgcactg cgttcggtaa tcgcggaaga
     9061 tggtaaggtg ctgtatcaga gcttcccgca ggcagaacgc gctgttccgg cgcaggcggc
     9121 gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacgggtc gtcagcttgg
     9181 ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga
     9241 tacctggttt gcaggcattg acggcagcac ggtgaccatc acctgggtag gccgtgataa
     9301 caaccagccg accaaactgt atggagccag cggggcaatg tcgatttatc agcgttatct
     9361 ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg cagatatggg
     9421 cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac
     9481 cagcgatccg caatcgctgt gccagcagag cgagatgcag cagcagccgt caggcaatcc
     9541 gtttgatcag tcttctcagc cgcagcaaca gccgcaacag caacctgctc agcaagagca
     9601 gaaagacagc gacggtgtag ccggttggat caaggatatg tttggtagta attaacatct
     9661 aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa taacatccca tctaagatat
     9721 taaccctttc ttttcatctg gttgtttatt aacccttcag gaacgctcag attgcgtacc
     9781 gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc attctcgttt
     9841 acgttatcat tcactttaca tcagagatat accaatggcg cgttccaaaa ctgctcagcc
     9901 aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg gcatgtctgt
     9961 ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc
    10021 gccgcaagaa agcgcatggg ggcctgctgc aactattgcg gcgcggcagt cagctaccgg
    10081 cactaaaacc gatacgccga ttcaaaaagt gccacagtct atttctgttg tgaccgccga
    10141 agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg cttagctaca cgccgggtgt
    10201 ctctgttggt acgcgtggcg catccaacac ctatgaccac ctgatcattc gcggttttgc
    10261 ggcagaaggc caaagccaaa ataactatct gaatggcctg aagttgcagg gcaacttcta
    10321 taacgatgcg gtcattgacc cgtatatgct ggaacgcgct gaaattatgc gtggcccggt
    10381 ttccgtgctt tacggtaaaa gcagtcctgg cggtctgttg aatatggtca gcaagcgtcc
    10441 gaccaccgaa ccgctgaaag aagttcagtt taaagccggt actgacagcc tgttccagac
    10501 tggttttgac tttagcgatg cgttggatga tgacggtgtt tactcttatc gcctgaccgg
    10561 tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa gagcagcgtt atgctattgc
    10621 accggcgttc acctggcgtc cggatgataa aaccaatttc accttccttt cttacttcca
    10681 gaacgagccg gaaaccggtt attacggctg gttgccgaaa gagggaaccg ttgagccgct
    10741 gccgaacggt aagcgtctgc cgacagactt taatgaaggg gcgaagaaca acacctattc
    10801 tcgtaatgag aagatggtcg gctacagctt cgatcacgaa tttaacgaca cctttactgt
    10861 gcgtcagaac ctgcgctttg gtgaaaacaa aacctcgcaa aacagcgttt atggttacgg
    10921 cgtctgctcc gatccggcga atgcttacag caaacagtgt gcggcattag cgccagcgga
    10981 taaaggccat tatctggcac gtaaatacgt cgttgatgat gagaagctgc aaaacttctc
    11041 cgttgatacc cagttgcaga gcaagtttgc cactggcgat atcgaccaca ccctgctgac
    11101 cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc tggtttggtt acgacgactc
    11161 tgtgccactg ctcaatctgt acaatccggt gaatcccgat ttcgacttca atgccaaaga
    11221 tccggcaaac tccggccctt accgcattct gaataaacag aaacaaacgg gcgtttatgt
    11281 tcaggatcag gcgcagtggg ataaagtgct ggtcacccta ggcggtcgtt atgactgggc
    11341 agatcaagaa tctcttaacc gcgttgctgg gacgaccgat aaacgtgatg acaaacagtt
    11401 tacctggcgt ggtggtgtta actacctgtt tgataatggt gtaacacctt acttcagcta
    11461 tagcgaatcg tttgaacctt cttcgcaagt tgggaaggat ggtaatattt tcgcaccgtc
    11521 taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg gaagatcgtc cgattgtagt
    11581 tactggtgcc gtgtataatc tcactaaaac caacaacctg atggcggacc ctgagggttc
    11641 cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc gtagaaatcg aagcgaaagc
    11701 ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac
    11761 caccgatact acctataaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct
    11821 gtgggctgac tataccttct ttgacggtcc gctttcaggt ttgacgctgg gcaccggtgg
    11881 tcgttatact ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac
    11941 ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt
    12001 ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc gccagctgct ttaacactta
    12061 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaacctttc gtttctaatt
    12121 tctcttttgg ggcacggatt tccgtgccca tttcacaagt tggctgttat gcaggaatac
    12181 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12241 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12301 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gtcgtcatca gcctccgtca
    12361 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc
    12421 cgcaaagtgg cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12481 ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg ggcgctttgg tgcggcagat
    12541 cgcgaaaagg tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    12601 gtcgatagtc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    12661 gatagccgtt gtctgttgct cgacgaaccg acttcggcgc tggatatcgc ccaccaggtt
    12721 gatgtgctgg cgctggtgca ccgtttaagt caggaacgtg gcctgacggt cattgccgtg
    12781 ttgcacgata tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcagtggt
    12841 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt
    12901 tatggcatcc cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    12961 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13021 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgacccc aatcgtattg
    13081 tggcgctgga gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg
    13141 tggcggatac catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga
    13201 ttgacgtcgg tttgcgtaca gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt
    13261 ttatggtctg gtcggcagga tatggccctt catcagaaat gctggcacgt attgcgccgg
    13321 gtcgcggatt taacttcagt gacggcaaac atccgttggc gatggcgcgt aaatcgctga
    13381 cggaaatggc agatttactt aacctgcaaa gcgcagcgga aacgcattta gcgcaatacg
    13441 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttactgc
    13501 tgacgacgct tatcgatccg cgccatatgc tggtcttcgg tccaaacagc ttgttccagg
    13561 aaattctcga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    13621 gtaccgccgt cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    13681 atcacgacaa cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc
    13741 cgtttgtccg tacagggcgc tttcagcgcg tacccgcagt ctggttttat ggtgcaacgc
    13801 tttcggcaat gcactttgtg cgtgttctgg ataacgccat cggaggtaaa gcgtgagtaa
    13861 acgaattgcg cttttcccgg cgttattgct ggcgctgtta gtgattgtcg ctacggcgct
    13921 cacctggatg aacttctcac aggcgctgcc gcgcagtcag tgggcgcagg ccgcctggtc
    13981 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgttgc cgcgtctggc
    14041 gatttcgctg ctggtgggcg cgggcctggg gctggtgggc gtgctgtttc agcaagtact
    14101 gcgtaacccg ctggcggaac cgacgacgtt gggcgttgca acaggcgcgc aactggggat
    14161 taccgtcacc acgctctggg cgatccctag cgcgatggcg agccagtttg ctgcgctggc
    14221 aggggcttgc gttgttggct taattgtctt tggcgtcgcg tgggggaaac ggctgtcgcc
    14281 ggtaacgctg attctcgcgg ggttggtagt gagcctttat tgcggcgcaa tcaatcagtt
    14341 actggttatc ttccatcatg accagttgca aagcatgttt ctgtggagca ctggaacgct
    14401 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    14461 gctgacgttg ctgctacttc gtccgttaac cctgatgggg cttgatgatg gcgtggcgcg
    14521 caatctcggg ctggccttgt cgcttgcgcg tctggcggcg ctgtcgctgg cgattgtcat
    14581 cagtgcgctg ctggtgaacg ctgtggggat tatcggcttt atcgggttgt tcgcgccgct
    14641 gctggcaaaa atgctggggg cgcggcgtct gctgccacga ctgatgctgg cgtcgttgat
    14701 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    14761 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgct
    14821 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtt aacgatcgtg tcgcgactga
    14881 acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg ctgttgttga tggctgtggt
    14941 ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgttgct
    15001 cgaggattta atgccctggc gctggccgcg aattatggcg gcgctgtttg cgggcgtcat
    15061 gctggcggta gcgggctgta ttattcagcg actgaccggc aacccgatgg caagcccgga
    15121 agtgctgggg attagctccg gcgcggcgtt tggtgtggtg ttaatgctgt ttctggtacc
    15181 gggtaatgcc tttggctggt tgttacccgc aggcagtctc ggggcggcgg tgatgctgtt
    15241 gatcattatg atcgccgccg gacgcggtgg attttcccca caccgcatgt tactggcggg
    15301 gatggcgtta agtaccgcgt tcaccatgct tttgatgatg ttgcaggcaa gtggtgaccc
    15361 gcgaatggcg caagtgctga cctggatttc cggttcgacc tacaacgcga ccgatgctca
    15421 ggtctggcgc accggaattg tgatggtgat tttgctggcg attactccgc tgtgccgccg
    15481 ctggctgacc attttaccgc tgggtggtga taccgcgcgg gcggtaggga tggcgctgac
    15541 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    15601 tattggaccg ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggctt
    15661 tcgacggacg atgccacata tcgtaatttc ggcgctggtg ggtggtttac tgctggtgtt
    15721 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atcccggcgg ggctgctgtc
    15781 gacctttatc ggtgcgccat attttatcta tttgctgaga aagcagagcc gttaatagaa
    15841 tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg cgaacacccg acgtgcagca
    15901 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac
    15961 gctgacggtg ccaggtaaac accttcatcc agcatcatat ggaagaaacg cttaaaacgc
    16021 tcgacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaag
    16081 ataccgaaca taccaccgac gtggttaacg accagcggaa ttcccgcttc ttctgccgct
    16141 tccagcagac cttctgccag acgtgttgtc agctcatcca gcgtttcgtg aatgcccggc
    16201 tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg caatcgggtt accggaaagc
    16261 gtacccgcct gatagaccgg acctgtaggg gccagcgcat ccattacatc acgacgacca
    16321 ccgaatgcgc ctaccggcat tccaccgccg atgatttttc cgaggcaggt taaatctggc
    16381 actacgccga aataatcctg tgcgccagct agcgctacgc ggaaaccggt catcacttca
    16441 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac gcagacctgg caggaactct
    16501 ggcagcggcg gaacacagtt catattgcct gccaccggct cgacgataat acaggcaatc
    16561 tcttgcgggt attgctcaaa cgcggcgcgt acagaggcca gatcgttata agtacaggtt
    16621 aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt ttggctggcc taacgtgagt
    16681 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    16741 ttaatgattt tgtcgcgacc ggtaaaacca cgggccaagc ggatggcgct catcgtcgcc
    16801 tcggtgccgg agttcaccat gcgcaccata tctatggtcg ggaccagttc ggtcatcagt
    16861 tgcgccattt tcacttccat ttcggtcggt gcaccaaagc ttaaaccacg ctcggcggct
    16921 tcaatcacgg cattgcggat cgccggatgg ttatggccca gcaccatcgg cccccaggaa
    16981 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17041 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    17101 ccagggatca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17161 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    17221 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17281 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17341 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17401 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    17461 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgctta tcgggctggc
    17521 tgcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    17581 acatactgct gataattatc cgctactgtt aaccgtcgct tttctctgtt cggcggtgct
    17641 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc gaagcagggg gatcggggat
    17701 cccggaaatt gaaggggcgc tggaagatca acgtcctgtt cgctggtggc gtgtattgcc
    17761 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggtgctag ggcgcgaagg
    17821 accaaccgtg cagatcggcg gcaatatcgg tcgcatggtg cttgatattt tccgcctgaa
    17881 aggtgatgaa gctcgccata cgctgctggc aaccggtgct gctgcggggc tggctgcggc
    17941 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18001 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18061 ccggattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    18121 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18181 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    18241 caaatgggta ctgatggggg gggcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18301 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag
    18361 catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt accaccttac tctgcttctc
    18421 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    18481 cgctttcgga atggttgccg ttgagctgtt tccgcaatat cacctggagg cggggacgtt
    18541 tgctattgcc ggaatggggg cattactggc ggcatctatt cgcgcgccgt taacggggat
    18601 cattctggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    18661 tcttggcgca acactattag cgcaatttac cggcgggaaa cctctatact cggcgattct
    18721 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    18781 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    18841 gaatttgccc gattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    18901 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    18961 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19021 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19081 cctggtggtt gatccgatga gcctgcaata tctggtcggc ggttccgttg actacaccga
    19141 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19201 cggttcttcc tttagcatct aatctgttgt tcatgtgccg gataaactat ccggcacatc
    19261 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19321 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19381 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19441 gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg ccagtacatc acgaatgatc
    19501 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    19561 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    19621 ggcaacatcc attttggtaa gcgtcttggc tggcgcacca gcacgatggt cagcatgctg
    19681 gtgaccatgg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    19741 agcgccatgt cgcgaattgt tccgtcgcct actgcggtaa ccacgcccag taccagaaca
    19801 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cggagatggc aaataccgct
    19861 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctgaaaa
    19921 ggtcaggcac taatctacct gtgaaagcgc attacagagc tgttgtgctg cgaggataat
    19981 acgtgggctt gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg
    20041 ttcaccccag tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc
    20101 ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc
    20161 tttaaagatg ttttctccgc cacaaacttc gagtacctgg ttctgaatcg actcttttcc
    20221 actggtaaat ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc
    20281 atattgcgct ttcaattgca cgtactgatc cagcagggat tgcgcggctt gttcggcctt
    20341 gtctggttgc ggactccagg gggccagttg acgtaacgca ttggcaattt gttcaatgct
    20401 tgtcgcatcg acccacatca cttttattcc cagcgaagcc agctggtcaa cctgccgctc
    20461 ggcattacct ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag
    20521 attcatcccc tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga
    20581 atagctgctg accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg
    20641 agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt ggcgcgagaa aagacagggc
    20701 gaccagcgcc ctgaacagtg atttagccat gtgccagttt ctgcaccagt gactcaacca
    20761 tcaggctgga ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt
    20821 gatcggccac gtcggagatg gcgcgtacga caacaaacgg gacgttgaaa ttgtggcaga
    20881 catgggcgat tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga
    20941 ttttcgccag accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta
    21001 cagcgttaag attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag
    21061 ctttaaagcc tgtcggacag cctggtaact gaccgtattc ataaccaaat gccgtgacat
    21121 ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg
    21181 ccaggccacc ggcagaaccg gtgttaataa tcacatctgg cttgcagtgt tccaacagca
    21241 gagtggcacc cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg
    21301 ttccattcag ttggccggta tagatttcgc aaccgccgag actgatagtt tgacggtttt
    21361 cgattttgtc acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca
    21421 tagatttact cgcgataagc ccgatttgaa ggcatagttt accatgcgct tacggggaag
    21481 cgtatttctc acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg
    21541 catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac atgagatcct gcggatcttc
    21601 gagagcgatc gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc
    21661 caggtttttc cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa
    21721 gtccagcagg tgggacgcta catcgccaaa gaaattttaa gccgtctgaa agagcttaaa
    21781 ttgctggaag catacggcct ggatgaactg accggcccct ttgaaagcat tgttgagatg
    21841 tcatgcctga tgcacgatat cggcaatccg ccgtttggtc attttggcga agcagcgata
    21901 aatgactggt ttcgccaacg tttgcacccg gaagatgccg aaagccagcc tctgactgac
    21961 gatcgctgca gcgtggcggc actacgttta cgggacgggg aagaaccgct taacgagctg
    22021 cggcgcaaga ttcgtcagga cttatgtcat tttgagggga atgcacaagg cattcgtctg
    22081 gtgcatacat tgatgcggat gaatctcact tgggcacagg ttggcggtat tttaaaatat
    22141 acccgtccgg cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag
    22201 ccgggttatt atctttctga agaagcctat attgcccggt tgcgtaaaga acttaatttg
    22261 gcgctttaca gtcgttttcc attaacgtgg attatggaag ctgccgacga catctcctat
    22321 tgtgtggcag accttgaaga tgcggtagag aaaagaatat ttaccgttga gcagctttat
    22381 catcatttgc acgaagcgtg gggccagcat gagaaaggtt cgctcttttc gctggtggtt
    22441 gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc gcagtacgga agatcagttt
    22501 tttatgtatt tacgggtaaa caccctaaac aaactggtac cctacgcggc acaacgattt
    22561 attgataatc tgcctgcgat tttcgccgga acgtttaatc atgcattatt ggaagatgcc
    22621 agcgaatgca gcgatcttct taagctatat aaaaatgtcg ctgtaaaaca tgtatttagc
    22681 catccggatg tcgagcagct tgaattgcag ggctatcggg tcattagcgg attattagag
    22741 atttatcgtc ctttattaag cctgtcgtta tcagacttta ctgaactggt agaaaaagaa
    22801 cgggtgaaac gtttccctat tgagtcgcgc ttattccaca aactctcgac gcgccatcgg
    22861 ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg
    22921 gaatattatt accgttgccg cctgctgcag gattatatca gcggtatgac cgacctctat
    22981 gcgtgggatg aataccgacg tctgatggcc gtagaacaat aaccaggctt ttgtaaagac
    23041 gaacaataaa tttttacctt ttgcagaaac tttagttcgg aacttcgggc tataaaacga
    23101 atctgaagaa cacagcaatt ttgcgttatc tgttaatcga gactgaaata catgaaaaaa
    23161 accacattag cactgagtgc actggctctg agtttaggtt tggcgttatc tccgctctct
    23221 gcaacggcgg ctgagacttc ttcagcaacg acagcccagc agatgccaag ccttgcaccg
    23281 atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg tagaaggtag cacaaccgtt
    23341 aatacgccgc gtatgccgcg taatttccag cagttcttcg gtgatgattc tccgttctgc
    23401 caggaaggtt ctccgttcca gagctctccg ttctgccagg ggggccaggg cggtaatggc
    23461 ggcggccagc aacagaaatt catggcgctg ggttccggcg tcatcattga tgccgataaa
    23521 ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga cggtgattaa agtccagctg
    23581 agcgatggtc gtaagttcga cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg
    23641 ctgatccaaa tccagaaccc gaaaaaactg accgcaatta agatggcgga ttctgatgca
    23701 ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt ttggtctggg cgagacggta
    23761 acttccggga ttgtctctgc gctggggcgt agcggcctga atgctgaaaa ctacgaaaac
    23821 ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg gtggtgcgct ggttaacctg
    23881 aacggcgaac tgatcggtat caacaccgcg atcctcgcac cggacggcgg caacatcggt
    23941 atcggttttg ctatcccgag taacatggtg aaaaacctga cctcgcagat ggtggaatac
    24001 gggcaggtga aacgcggtga gctgggtatt atggggactg agctgaattc cgaactggcg
    24061 aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa gccaggttct gcctaattcc
    24121 tcggctgcaa aagcgggcat taaagcgggt gatgtgatca cctcactgaa cggtaagccg
    24181 atcagcagct ttgccgcact gcgtgctcag gtgggtacta tgccggtggg cagcaaactg
    24241 accctgggct tactgcgcga cggtaagcag gttaacgtga acctggaact gcagcagagc
    24301 agccagaatc aggttgattc cagctccatc ttcaacggca ttgaaggcgc tgagatgagc
    24361 aacaaaggta aagatcaggg cgtggtagtg aacaacgtga agacgggcac tccggctgcg
    24421 cagatcggcc tgaagaaagg tgatgtgatt attggtgcga accagcaggc agtgaaaaac
    24481 atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg tgctggcact gaacattcag
    24541 cgcggcgaca gcaccatcta cctgttaatg cagtaatctc cctcaacccc ttcctgaata
    24601 cgggaagggg ttctccttac aatctgtgaa cttcgccaca actccataca tcttcatcat
    24661 cctttgggca tttgcacaat gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa
    24721 cggaggactt atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg
    24781 taccatgcgc atcatcgata ccaatatcaa cgtaatggat gcccgcgggc gaattatcgg
    24841 cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt gcattgctgg tactttcaca
    24901 gggacgagtc gtcgatatcg atgacgcggt agcacgtcat ctgcacggtg tgcggcaggg
    24961 gattaatcta ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga
    25021 accagagaat ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga
    25081 acagtcgcgg ttgatgcact tgttggcgca ggatagccgt ttgcgggaag aactggtgat
    25141 gaacctgatt caggcagagg agaatactcc cgcacttctg aatgggcgca acggctgggg
    25201 atcgatctca atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggc
    25261 gtggacagcg caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt
    25321 aataatctgg tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac
    25381 tcttttgggc gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc
    25441 atgaaagagt acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct
    25501 agcagtattg cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg
    25561 atgccagaaa gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt
    25621 ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg
    25681 gacaataacg gcttgctgcg acgaacgctg gcggcgtggt ttcgccacaa tgtgcaaccg
    25741 ctggcaacgt caaaggcgtt gtttattcat cgtaataccc tggagtatcg gcttaatcgt
    25801 atatcggaac tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg
    25861 gcgttacaac tggatgaaga gcggtagatt atgcgttaag gttgaggcgg cgctggctca
    25921 ttcccctcag cccgaagggg agaggggaat gcccgagtta accgtcaatt cgggattatt
    25981 tacgcgttag tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact
    26041 ccaggtgacg taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct
    26101 gacagatttg atcaagctca tcaatgatat accgtagatt cgggctgatt tcctggactt
    26161 ctttataacc ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa
    26221 acttaacgct cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat
    26281 cgttgttggc ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca
    26341 gacttttcag attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt
    26401 ttagcatttg gcttggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag
    26461 acttctatca tggcgtaaaa atcaaaaaat tacctgcttt attgtggcga tattccatgg
    26521 gatggattag cacatttatc ttcgttttgc gaattacatg acagtccgaa acaagatgaa
    26581 aaagtattta aaaaaggggg aggaagaatg agtaggacga attattctga gccagaaagc
    26641 atacgggcag catcatgctg cccgtatatg tactcttaat cgatggtacg cagcagttcg
    26701 ttaatgccga ctttgccgcg agttttcgcg tcaactttct taacgataac cgcacagtag
    26761 aggctgtatt tgccatcttt tgacggcaga ttacctgaaa caaccacaga ccccgccgga
    26821 acgcgaccgt agtggatttc gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg
    26881 tatacgccca tagagatgac ggaaccttct tcgacaatca ccccttcaac cacttcagag
    26941 cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt tagcctgcag cggctccagc
    27001 acgccgccga tgcccacgcc accggaaagg tggacgtttt taccaatctg cgcacaagaa
    27061 ccgacggtcg cccaggtatc aaccatggtg ccttcatcaa catatgcgcc gatgttaacg
    27121 taagacggca tcagcacggt gttacgggca ataaacgcac cctgacgtac cgccgctggt
    27181 ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt cgtagtcggc gaatttcatc
    27241 ggcactttgt cgaagtagcg gctttctgcc ccttcgatca cctgattatc attaatacgg
    27301 aaagagagca gcaccgcttt tttcaaccac tgatgcgtca cccactgacc gtcaattttt
    27361 tccgctacac gcagtgcgcc ggaatccagc agggcgatca cctgatttac cgcttcgcgg
    27421 gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata
    27481 atgttctgta actgctgcat tgttaaactc ttttcatatc agtaaacaca tcacccttta
    27541 tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta
    27601 agcgcacgcc ggtcagcggt ggcaattatg aataaatctt ctactcgctc gccaatggtt
    27661 gtaattcggg caccatgaag cgaaattccc agatcggcaa aaattttccc gactcgcgcc
    27721 agcagtccag gttggtcgag ggcgatcagt tcgaggaacg atttgcggtc ggtatgggtc
    27781 ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta atttggcggg ttggcgacgg
    27841 ggctgcggtg gctgccagct actttgcgtc agcacctgct ccagaccaaa ccgaataacc
    27901 tcatggcgat ctgcggacag cgggctgcca tcgggttcca gcacgataaa ggtatccatc
    27961 gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt gcggcggtct
    28021 aattcggcac agacggcggc aaacagatag gggcggtccg ggctccagat aaaaatctcg
    28081 gtgcctccac gcgtagcctg cgggctaagc aataccagcg gtttgcttaa atcatgctgt
    28141 aataaatggc gggcatgcca ggccagttga tttgggctat ggcggacaaa atagttagca
    28201 cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt
    28261 gccagtgcct ggagttggtg atggcgaacc cgttcgcgca tatccggcgt gttttgcatt
    28321 ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc gcaacagact ttgcttccag
    28381 ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca taccagatag
    28441 cgcagacgat tttccgtttg aacttcttcg gcaaactgct tgatgacttc agggtcctga
    28501 atatcgcggc gttgtgctgt caccgacatc agcaagtgct ggcgaaccag ccaggcaacc
    28561 agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa aatgcactac atcctgagca
    28621 ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg
    28681 aaaatcagct cagttgacgg caggcgcggc cagacgtcca cacacaacgg atggcgctgg
    28741 cgcgtttctt cactggcaaa actctccagt ttcagcatca cgcggatagt atgttcatcc
    28801 accgtgtagg cgtggaacag atcaaactgc atctgcccga cgatatgcga ccattgcggc
    28861 atatacgcgc cgagcacgct atggcgatgc attggcaata gcccgcgccg caccgctccg
    28921 gggtgacgca gaatgctcaa aaacagtttt cgtgcttgcg gaatattaca cagcggttgt
    28981 tgcagatggc gacgggcatg gcgtaactgg cgcagcgtgg tggagtaaat accggtgatc
    29041 gcactgttgc gcaccatggt gtagaacata cgcaagatgg cttccggctg gcgcataaat
    29101 agtgtttcat cacgcaggtc gattagcgta ccgcgtagct gaaactcatc gtcgagtgga
    29161 cgtggttttt cgtcggcggg aagggcgagg atggcttcat cgaacagttg cagcagcatc
    29221 tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat ccttcatcat ccgctcgacc
    29281 ggttcgttgc cttcaccact gtaattcaga cgctgagcga cgctaagttg gcgatcgaat
    29341 aacagacgat tatcgtaacg gctgacgacc agatgcaggg caaagcgaat acgccacagt
    29401 atatgcagac attcgtttaa ttccgcccgc tccgctgagg ttaagaagcc aaacccgacc
    29461 atttcatcca gcgatgttgc gccaaaatga cggcgggcca cccattgcag agtgtgaata
    29521 tcgcgcaagc cgccagggct acttttgatg tctggttcaa ggttgtagct ggtgccatgg
    29581 taacgctgat ggcgctggtt ctgttcttca actttcgctg cgtagaactt gtcggaaggc
    29641 cagaatcctt cgctaaaaat atgtttttgc agttcgagga agagcgcaac atcgccaatt
    29701 aataagcggg attcgattaa attggtggcg acggttaaat ccgataaccc ttccaacatg
    29761 cactcttcaa gcgtgcgcac actatgaccg acttccagct ttacatccca gagcagcgtt
    29821 aacagctcgc ccactttttg cgcctgatcg tccgggagct ttttacggct taaaatcagt
    29881 aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt agccaccgac ggcaaccaat
    29941 gccaggtcgg caatctggct gaatcccgct tcaatccata atcgttgcag gagctggtct
    30001 atgaactcgg tacgtgcctc aatcaactgt tctgcagaga tcccattgtc aaaggcatca
    30061 cccagccaac gctggaaagt atcgatatgg gcttttatcc cacagacggt taattcatca
    30121 cggggccagg cgcatggatt ttgcggttga ccgggcaggg tggagagagc ggtgtttgcg
    30181 tactgttctg gaagggtatt cattgtgcgc cacccataag attaaattat cgcattaaaa
    30241 aagccggcat tcgccggctt catcttattc gtcgtgcgag attatcgccg ggatggtgtc
    30301 atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc accacaatag tatgctcata
    30361 ttgtgcagac aagctgcgat ctttggtttt taccgtccag ccatctttca tggtgcggat
    30421 ctctttttta cccgcgttga ccattggctc aatggtgaac gtcatccccg gtttcagcac
    30481 gacgttggtt tcacgggagt catagtgcag cacctgcggt tcttcatgga agccgcgacc
    30541 aataccgtgt ccgcaatatt cacgaacgac ggagaagcct tctgcttcga caaatttctg
    30601 aatcgccgca ccgatttcgc gcagattaat gcctggtttt accatgcgta gcgccaggta
    30661 caggctttct tgcgtgatgc ggcacaggcg ttcgcccatg atggtcggct taccgacgat
    30721 aaacattttc gaggtatcgc cgtggaaacc atctttgatt acggtgacat caatgttaac
    30781 gatatcgcca tctttcagca gcttagcatc gtccgggata ccgtggcaca ccacttcatt
    30841 aatagagatg caaacggatt tcggatagcc gtgatagccg aggcaggcag aaaccgcgtg
    30901 ttgttcatta acaatgtaat cattacagat gcgatccagc tcgccggtgc tgacgcccgg
    30961 tttaacatac ggttcgatca tctccagcac ttcggcagcc agtcggccag cgacgcgcat
    31021 tttttcgata tcttctgggg tcttgattga gatagccatt aattctgtcc atcagcgtcg
    31081 gtgataccga caatatatat gtaagtgccg tcaatggtat cacacccggg caaattgaga
    31141 atcattctga atttcgccaa acgtgccact gaaggttttc tataatagaa aattcgacgt
    31201 ctgatgctgt acacagcgcc aacaattatt ggtgtccacg acgtatttgt ggtataaagc
    31261 gcgccggact tccgatccat ttcgtataca cagactggac ggaagcgaca atctcacttt
    31321 gtgtaacaac acacacgtat cggcacatat tccggggtgc cctttggggt cggtaatatg
    31381 ggatacgtgg aggcataacc ccaactttta tatagaggtt ttaatcatgg caactgtttc
    31441 catgcgcgac atgctcaagg ctggtgttca cttcggtcac cagacccgtt actggaaccc
    31501 gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt cacatcatca accttgagaa
    31561 aactgtaccg atgttcaacg aagctctggc tgaactgaac aagattgttt ctcgcaaagg
    31621 taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct
    31681 gagctgcgac cagttcttcg tgaaccatcg ctggctgggc ggtatgctga ctaactggaa
    31741 aaccgttcgt cagtccatca aacgtctgaa agacctggaa actcagtctc aggacggtac
    31801 tttcgacaag ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg agaaactgga
    31861 aaacagcctg ggcggtatca aagacatggg cggtctgccg gacgctctgt ttgtaatcga
    31921 tgctgaccac gaacacattg ctatcaaaga agcaaacaac ttgggtattc cggtatttgc
    31981 tatcgttgat accaactctg atccggacgg tgttgacttc gttatcccgg gtaacgacga
    32041 cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct gcaaccgtac gtgaaggccg
    32101 ttctcaggat ctggcttccc aggcggaaga aagcttcgta gaagctgagt aataaggctt
    32161 gataactccc ccaaaatagt tcgagttgca gaaaggcggc aagctcgaga attcccggga
    32221 gcttacatca gtaagtgacc gggatgagcg agcgaagaca acgcatctgc ggcgcgaaat
    32281 atgaaggggg agagccctta tagaccaggt agtacacgtt tggttagggg gcctgcatat
    32341 ggcccccttt ttcactttta tatctgtgcg gtttaatgcc gggcagatca catctccgag
    32401 gattttagaa tggctgaaat taccgcatcc ctggtaaaag agctgcgtga gcgtactggc
    32461 gcaggcatga tggattgcaa aaaagcactg actgaagcta acggcgacat cgagctggca
    32521 atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg caacgttgct
    32581 gctgacggcg tgatcaaaac caaaatcgac ggcaactacg gcatcattct ggaagttaac
    32641 tgccagactg acttcgttgc aaaagacgct ggtttccagg cgttcgcaga caaagttctg
    32701 gacgcagctg ttgctggcaa aatcactgac gttgaagttc tgaaagcaca gttcgaagaa
    32761 gaacgtgttg cgctggtagc gaaaattggt gaaaacatca acattcgccg cgttgctgcg
    32821 ctggaaggcg acgttctggg ttcttatcag cacggtgcgc gtatcggcgt tctggttgct
    32881 gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag
    32941 ccagaattca tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga ataccaggta
    33001 cagctggata tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa
    33061 ggccgcatga agaaattcac cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa
    33121 ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg ctgaagtgac tggcttcatc
    33181 cgtttcgaag tgggtgaagg catcgagaaa gttgagactg actttgcagc agaagttgct
    33241 gcgatgtcca agcagtctta attatcaaaa aggagccgcc tgagggcggc ttctttttgt
    33301 gcccatcttg taaattcagc taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt
    33361 cgctagtatt aattcatttc aatcgttgac agtctcagga aagaaacatg gctaccaatg
    33421 caaaacccgt ctataaacgc attctgctta agttgagtgg cgaagctctg cagggcactg
    33481 aaggcttcgg tattgatgca agcatactgg atcgtatggc tcaggaaatc aaagaactgg
    33541 ttgaactggg tattcaggtt ggtgtggtga ttggtggggg taacctgttc cgtggcgctg
    33601 gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca catggggatg ctggcgaccg
    33661 taatgaacgg cttggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga
    33721 tgtccgctat tccattgaat ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc
    33781 tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac aggtaacccg ttctttacca
    33841 ccgactcagc agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa
    33901 ccaaagttga cggcgtgttt accgctgatc cggcgaaaga tccaaccgca accatgtacg
    33961 agcaattgac ttacagcgaa gtgctggaaa aagagctgaa agtcatggac ctggcggcct
    34021 tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt caatatgaac aaaccgggtg
    34081 cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt aatcacggaa taattcccgt
    34141 gatggataaa taagggtaag attccgcgta agtatcgcgg gggcgtaagt ctggttataa
    34201 ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc agcaaccgga gcgtacaaaa
    34261 gtacgtgagg atggcgagca ctgctcgggg ccaaaatggc aaataaaata gcctaataat
    34321 ccagacgatt acccgtaata tgtttaatca gggctatact tagcacactt ccactgtgtg
    34381 tgactgtctg gtctgactga gacaagtttt caaggattcg taacgtgatt agcgatatca
    34441 gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc gttcaaaacc caaatcagca
    34501 aaatacgcac gggtcgtgct tctcccagcc tgctggatgg cattgtcgtg gaatattacg
    34561 gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt agaagattcc cgtactctga
    34621 aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga aaaagcgatt atggcctccg
    34681 atctcggcct gaacccgaac tctgcgggta gcgacatccg tgttccgctg ccgccgctga
    34741 cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg tgaagcagaa caagcgcgtg
    34801 ttgcagtacg taatgtgcgt cgtgacgcga acgacaaagt gaaagcactg ttgaaagata
    34861 aagagatcag cgaagacgac gatcgccgtt ctcaggacga tgtacagaaa ctgactgatg
    34921 ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga agcagaactg atgcagttct
    34981 gatttcttga acgacaaaaa cgccgctcag tagatccttg cggatcggct ggcggcgttt
    35041 tgctttttat tctgtctcaa ctctggatgt ttcatgaagc aactcaccat tctgggctcg
    35101 accggctcga ttggttgcag cacgctggac gtggtgcgcc ataatcccga acacttccgc
    35161 gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg tagaacagtg cctggaattc
    35221 tctccccgct atgccgtaat ggacgatgaa gcgagtgcga aacttcttaa aacgatgcta
    35281 cagcaacagg ggagccgcac cgaagtctta agtgggcaac aagccgcttg cgatatggca
    35341 gcgcttgagg aggttgatca ggtgatggca gccattgttg gtgctgctgg gctgttacct
    35401 acgcttgctg cgatccgcgc gggtaaaacc attttgctgg ccaataaaga atcactggtt
    35461 acctgcggac gtctgtttat ggacgccgta aagcagagca aagcgcaatt gttaccggtc
    35521 gatagcgaac ataacgctat ttttcagagt ttaccgcaac ctatccagca taatctggga
    35581 tacgctgacc ttgagcaaaa tggcgtggtg tccattttac ttaccgggtc tggtggccct
    35641 ttccgtgaga cgccattgcg cgatttggca acaatgacgc ccgatcaagc ttgccgtcat
    35701 ccgaactggt cgatgggacg taaaatttct gtcgattcgg ctaccatgat gaacaaaggt
    35761 ctggaataca ttgaagcgcg ttggctgttt aacgccagcg ccagccagat ggaagtgctg
    35821 attcacccgc agtcagtgat tcactcaatg gtgcgctatc aggacggcag tgttctggcg
    35881 cagctggggg aaccggatat gcgtacgcca attgcccaca ccatggcatg gccgaatcgc
    35941 gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa gtgcgttgac atttgccgca
    36001 ccggattatg agcgttatcc atgcctgaaa ctggcgatgg aggcgttcga acaaggccag
    36061 gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg ttgctgcttt tcttgcgcaa
    36121 caaatccgct ttacggatat cgctgcgttg aatttatctg tactggaaaa aatggatatg
    36181 cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg cgaacgcgag tgaagtcgcc
    36241 agaaaagagg tgatgcgtct cgcaagctga ggataatccg gctacagaga gtcgcgctat
    36301 ttgttagcgt agggcttcaa tgatatagtc tgcgccatct gatcgtaagt agttggcttt
    36361 ataaggtcag gtatgccgtg gttttacacg gctttttttt gtataggctt cagtattcct
    36421 gagtaccgta aaccctgtca gggaataaaa aacgcgtgat gttgtctgct actcaaccac
    36481 ttagcgaaaa attgccagcg catggctgcc gtcatgttgc gatcattatg gacggcaatg
    36541 gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg gcataaagcc ggggcaaaat
    36601 ccgtccgccg ggctgtctct tttgcggcca acaacggtat tgaagcgtta acgctgtatg
    36661 cctttagtag tgaaaactgg aaccgaccag cgcaggaagt cagtgcgtta atggaactgt
    36721 ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg acataacgtg cgtctgcgta
    36781 ttattggcga taccagtcgt tttaactcgc gtttgcaaga acgtattcgt aaatctgaag
    36841 cgctaacagc cggaaatacc ggtctgacgc tgaatattgc ggcgaactac ggtggacgtt
    36901 gggatatagt ccagggagtc aggcaactgg ctgaaaaggt gcagcaagga aacctgcaac
    36961 cagatcagat agatgaagag atgttaaacc agcatgtctg tatgcatgaa ctggcccctg
    37021 tagatttagt aattaggact gggggagagc atcgcattag taactttttg ctttggcaaa
    37081 ttgcctatgc cgaactttac tttacagatg ttctctggcc cgatttcgat gaacaagact
    37141 ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg tttcggcggc accgagcccg
    37201 gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta tcgcctgata tctgcttttg
    37261 tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc gccggtgggg ttcgccattg
    37321 taacgctggt ggtctgcatg ctggcagcgt gggaatgggg acagcttagc ggttttacca
    37381 ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt attgttggcg ctgatgcttt
    37441 ttctgttgcc ggaatatcac cgaaatattc atcaaccgct ggttgaaatc tcactttggg
    37501 cttcgttggg ttggtggatt gtcgcgctat tgctggtgct gttttaccca ggttccgcag
    37561 caatctggcg taactctaaa acattgcgcc ttatttttgg cgtgctaacc attgttccct
    37621 tcttctgggg catgctggcg ttacgggcct ggcactatga cgagaatcat tacagtggcg
    37681 caatatggct gctctatgtc atgatcctgg tatggtgcgc tgactccggc gcatatatgt
    37741 ttggcaaatt gtttggtaaa cataagctgg caccgaaggt ttctccgggt aaaacctggc
    37801 aaggctttat aggtggactc gctactgcag cggtaatctc atggggttat ggcatgtggg
    37861 cgaatctcga cgtcgctccc gtcaccttac tcatttgctc tattgtcgca gcgttagcct
    37921 cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga agcaggaatt aaggacagcg
    37981 gtcatttaat tccaggacac ggtggtattt tagatcgtat tgatagcctg acggctgcgg
    38041 taccggtctt tgcttgcttg ttgttactgg tattcaggac gctttaacgg aaggtaatat
    38101 gctgagtttt ctctgggatt tggcttcgtt catcgttgca ctgggtgtac ttatcaccgt
    38161 gcatgaattt ggtcatttct gggttgcccg gcgttgtggt gttcgcgttg agcgtttctc
    38221 aatagggttt ggtaaggcgc tctggcggcg aactgataag ctcggcaccg aatatgttat
    38281 cgccctgatc ccgttgggcg gttatgtcaa aatgctggat gagcgcgcag aaccggtcgt
    38341 tccggaactc cgccaccatg ccttcaataa taaatctgtc ggccaacgag tggcgattat
    38401 tgccgcaggt ccggttgcaa acttcatttt tgctatcttt gcctactggc tggtttttat
    38461 tattggtgtg cctggcgtac gtccggtggt tggcgaaata gcagccaatt cgatagctgc
    38521 ggaagcacaa attgcaccag gtacggaact aaaagccgta gatggtatcg aaacgcctga
    38581 ttgggatgcc gtgcgtttgc agttggtcga taaaattggc gatgaaagca ccaccattac
    38641 agtagcgcca tttggcagcg accaacggcg ggatgtaaag ctcgatttac gtcactgggc
    38701 gtttgagcct gataaagaag atccggtatc ttcgctgggg attcgtcctc gtgggccgca
    38761 aattgaacct gtactggaaa atgtgcagcc aaactcggcg gcaagcaagg caggtttgca
    38821 agcaggcgac aggatcgtta aagtcgatgg tcagccctta acgcagtggg tgacctttgt
    38881 gatgcttgtc cgggataacc cgggtaaatc cttagcgtta gaaatcgaaa ggcaggggag
    38941 tcccttgtct ttgacattaa tcccggagag taaaccgggt aatggtaaag cgattggttt
    39001 tgtcggtatt gagccgaaag tcattccttt gccagatgag tataaagttg tacgccagta
    39061 tgggccgttc aacgccatcg tcgaagccac ggacaaaacg tggcagctga tgaagctgac
    39121 ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa ctgaacaacc tcagtgggcc
    39181 gatttctatc gccaaggggg ctgggatgac agcggaactc ggggtagttt attacctgcc
    39241 gtttcttgcg cttattagcg tgaacttagg gataattaac ctgtttccgt tgcccgtact
    39301 tgacgggggg catctgctgt tccttgcgat cgaaaagatc aagggcggac cggtatccga
    39361 gcgggttcaa gacttttgtt atcgcattgg ctcgattctg ctggtgctgt taatggggct
    39421 tgcacttttc aatgatttct ctcggttatg agagttagtt aggaagaacg cataataacg
    39481 atggcgatga aaaagttgct catagcgtcg ctgctgttta gcagcgccac cgtatacggt
    39541 gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc ttcagcgtgt cgccgttggt
    39601 gcggccctcc tcagtatgcc ggtgcgtaca ggcgacacgg ttaatgatga agatatcagt
    39661 aataccattc gcgctctgtt tgctaccggc aactttgagg atgttcgcgt ccttcgtgat
    39721 ggtgataccc ttctggttca ggtaaaagaa cgtccgacca ttgccagcat tactttctcc
    39781 ggtaacaaat cggtgaaaga tgacatgcta aagcaaaacc tcgaggcttc tggtgtgcgt
    39841 gtgggcgaat ccctcgatcg caccaccatt gccgatatcg agaaaggtct ggaagacttc
    39901 tactacagcg tcggtaaata tagcgccagc gttaaagctg tcgtgacccc gctgccgcgc
    39961 aaccgtgttg acctaaaact ggtgttccgg gaaggtgtgt cagctgaaat ccagcaaatt
    40021 aacattgttg gtaaccatgc tttcaccacc gacgaactga tctctcattt ccaactgcgt
    40081 gacgaagtgc cgtggtggaa tgtggtaggc gatcgtaaat accagaaaca gaaactggcg
    40141 ggcgaccttg aaaccctgcg cagctactat ctggaccgcg gttatgcccg tttcaacatc
    40201 gactctaccc aggtcagtct gacgccagat aaaaaaggta tttacgtcac ggtgaacatc
    40261 accgaaggcg atcagtacaa gctttctggc gttgaagtga gcggcaacct tgccgggcac
    40321 tccgctgaaa ttgagcagct gactaagatc gagccgggcg agctgtataa cggcactaaa
    40381 gtgaccaaaa tggaagatga cattaaaaag cttctcggtc gctatggtta tgcctatccg
    40441 cgcgtacagt cgatgcctga aattaacgat gccgacaaaa ccgttaaatt acgcgtgaac
    40501 gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt ttgaaggtaa cgatacctcg
    40561 aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag gtgcatggtt ggggagcgat
    40621 ctggtcgatc agggtaagga gcgtctgaat cgtctgggct tctttgaaac tgtcgatact
    40681 gatacccaac gtgttccggg tagcccggac caggttgatg tagtctacaa ggtgaaagaa
    40741 cgtaacactg gtagcttcaa cttcgggatt ggttacggta ctgaaagtgg cgtgagcttc
    40801 caggctggcg tgcaacagga taactggtta ggtacaggtt atgctgttgg tatcaacggg
    40861 accaaaaacg attaccagac ctatgctgaa ctgtcggtaa ccaacccgta cttcaccgta
    40921 gatggcgtaa gcctcggtgg tcgtctcttc tataatgact tccaggcaga tgacgccgac
    40981 ctgtccgact ataccaacaa gagttatggt acagacgtga cgttgggctt cccgattaac
    41041 gaatataact cgctgcgtgc aggtctgggt tatgtacata actccctgtc caacatgcag
    41101 cctcaggttg cgatgtggcg ttatctgtac tctatgggtg aacatccgag cacttctgat
    41161 caggataaca gcttcaaaac ggacgacttc acgttcaact atggttggac ctataacaag
    41221 cttgaccgtg gttacttccc gacagatggt tcacgtgtca acctgaccgg taaagtgacc
    41281 attcctggat cggataacga atactacaaa gtgacgttag acacggcgac ttatgtgccg
    41341 atcgatgacg atcacaaatg ggttgttctg ggtcgtaccc gctggggtta tggtgatggt
    41401 ttaggcggca aagagatgcc gttctacgag aacttctatg ccggtggttc cagcaccgtg
    41461 cgtggcttcc agtccaatac cattggtccg aaagcagttt acttcccgca tcaggccagt
    41521 aattatgatc cggactatga ttacgaatgt gcgactcagg acggcgcgaa agacctgtgt
    41581 aaatcggatg atgctgtagg cggtaacgcc atggcggttg ccagcctcga gttcatcacc
    41641 ccgacgccgt ttattagcga taagtatgct aactcggttc gtacttcatt cttctgggat
    41701 atgggtaccg tttgggatac aaactgggat tccagccaat attctggata tccggactat
    41761 agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat tacaatggat gtccccattg
    41821 gggccgttgg tgttctccta cgcccagccg ttcaaaaagt acgatggaga caaggcagaa
    41881 cagttccagt ttaacatcgg taaaacctgg taagtgttct ccacaaagga atgtagtggt
    41941 agtgtagcga tgactttagg cgatcaatat aagatcgccg ggccacgcaa agaactgcac
    42001 cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa aagtggttat tagctgcagg
    42061 tcttggttta gcactggcaa cttctgctca ggcggctgac aaaattgcaa tcgtcaatat
    42121 gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt tctaacacgc tggaaaatga
    42181 gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc gatctgcagg ctaaaatgaa
    42241 aaaactgcag tccatgaaag cgggcagcga tcgcaccaaa ctggaaaaag acgtgatggc
    42301 tcagcgccag acttttgctc agaaagcgca ggcttttgag caggatcgcg cacgtcgttc
    42361 caacgaagaa cgcggcaaac tggttactcg tatccagact gctgtgaaat ccgttgccaa
    42421 cagccaggat atcgatctgg ttgttgatgc aaacgctgtt gcttacaaca gcagcgatgt
    42481 aaaagacatc actgccgacg tactgaaaca ggttaaataa gtaatgcctt caattcgact
    42541 ggctgattta gcgcagcagt tggatgcaga actacacggt gatggcgata tcgtcatcac
    42601 cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt acgttcatgg ttaacccaaa
    42661 ataccgtgag catttaggct tgtgccaagc gtccgcggtt gtcatgaccc aggacgatct
    42721 tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc tacctgactt acgcgcgcat
    42781 ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac attgcaccca gtgcggtgat
    42841 cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc gctaacgcgg tgattgagtc
    42901 cggcgttgaa ctgggcgata acgtgattat cggtgccggt tgcttcgtag gtaaaaacag
    42961 caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc atttaccatg agatccagat
    43021 cggtcagaat tgcctgatcc agtccggaac agtggtaggc gcagacggct ttggttatgc
    43081 caacgatcgt ggtaactggg tgaagatccc acagattggt cgcgtaatta ttggcgatcg
    43141 agtggagatc ggtgcctgca caaccatcga tcgcggcgcg ctggatgaca ctgttattgg
    43201 caatggcgtg atcattgata accagtgcca gattgcacat aacgtcgtga ttggcgacaa
    43261 tacggcggtt gccggtggcg tcattatggc gggcagcctg aaaattggtc gttactgcat
    43321 gatcggcgga gccagcgtaa tcaacgggca tatggaaata tgcgacaaag tgacggttac
    43381 gggcatgggt atggtgatgc gtcccatcac tgaaccaggc gtctattcct caggcattcc
    43441 gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg gtgatgaaca ttgatgacat
    43501 gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa caagactaac gttccatctt
    43561 ttgttcgcca aactttacgg cctgtctcat tcttacgatt gcggcaggcc gtgttattat
    43621 tgtcgtttct tatattttga caggaagagt atcttgacta ctaacactca tactctgcag
    43681 attgaagaga ttttagaact tctgccgcac cgtttcccgt tcttactggt ggatcgcgtg
    43741 ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa atgtctctgt caatgagcca
    43801 ttcttccagg gccatttccc tggaaaaccg attttcccgg gtgtgctgat tctggaagca
    43861 atggcacagg caacaggtat tctggcgttt aaaagcgtag gaaaactgga accgggtgag
    43921 ctgtactact tcgctggtat tgacgaagcg cgcttcaagc gcccggtcgt gcctggcgat
    43981 caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg gcctgacccg ttttaaaggg
    44041 gttgctctgg tcgatggtaa agtagtttgc gaagcaacga tgatgtgtgc tcgtagccgg
    44101 gaggcctgat acgtgattga taaatccgcc tttgtgcatc caaccgccat tgtggaagag
    44161 ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt gtatcgttgg accccatgtc
    44221 gaaattggtg agggtaccgt actgaaatct cacgttgtcg tgaatggtca tactaaaatt
    44281 ggccgcgata atgagattta tcagtttgcc tccatcggcg aagttaacca ggatttgaaa
    44341 tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta accgcattcg cgaaagcgtc
    44401 accattcatc gtggcacagt ccagggcggt ggattgacga aggtgggcag cgacaactta
    44461 ctgatgatca acgcgcacat tgcgcacgat tgtacggtag gtaaccgctg tattctcgcc
    44521 aacaacgcaa cgctggcggg tcacgtatcg gttgacgact tcgcgatcat cggcggcatg
    44581 accgcagtcc atcagttctg catcattggt gcgcatgtga tggttggcgg ctgctccggt
    44641 gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta accacgcaac gccgttcggt
    44701 gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg aggcgattac cgctatccgc
    44761 aatgcgtata agctaattta tcgtagcggt aaaacgctcg atgaagtgaa accggaaatt
    44821 gctgaactgg cggaaacata tccggaagtg aaagccttta ccgatttctt tgcacgctca
    44881 acgcgcggtc tgattcgtta atgactgacc agcgtccatt aacgattgcc ctggtcgccg
    44941 gagaaatctc cggcgatatc ctgggggccg gtttaatccg cgctctgaaa gaacgtgtgc
    45001 ccaacgcccg ctttgttggt gttgccgggc cacgaatgca ggctgaaggc tgcgaagcct
    45061 ggtacgaaat ggaagaactg gcggtgatgg gcattgttga agtgctcggt cgtctgcgtc
    45121 gcttactgca tattcgtgcc gatctgacaa agcgttttgg cgaactgaag ccagatgttt
    45181 ttgttggtat tgatgcgccc gacttcaata ttactcttga aggtaacctc aaaaagcagg
    45241 gtatcaaaac cattcattac gtcagtccat ccgtctgggc gtggcgacag aaacgcgttt
    45301 tcaaaatagg cagagccacc gatctggtgc tcgcatttct gcctttcgaa aaagcgtttt
    45361 atgacaaata caatgtaccg tgccgcttta tcggtcatac catggctgat gccatgccgt
    45421 tagatccaga taaaaatggt gcccgtgatg tgctggggat cccttacgat gcccactgtc
    45481 tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat gcttagtgcc gatttcttga
    45541 aaactgccca gcttttgcgc cagacatatc cggatctcga aatcgtggtg ccgctggtga
    45601 atgccaaacg ccgcgagcag tttgaacgca tcaaagctga agtcgcgcca gacctgtcgg
    45661 ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc cagcgatgcg gcactactgg
    45721 cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg cccgatggtg gtgggatatc
    45781 gcatgaagcc ttttaccttc tggttggcga agcggctggt gaaaactgat tatgtctcgc
    45841 tgccaaatct gctggcgggc agagagttag tcaaagagtt attgcaggaa gagtgtgagc
    45901 cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa cgggaaaacc agccacgcga
    45961 tgcacgatac cttccgtgaa ctgcatcagc agatccgctg caatgccgat gagcaggcgg
    46021 cacaagccgt tctggagtta gcacaatgat cgaatttgtt tatccgcaca cgcagctggt
    46081 tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc gcggtcgtca ccgctgcggt
    46141 gatccttgac ccggcgcgcc cgattgccgg gctgaatgat tccaaaaagc tgagcgaaaa
    46201 acgccgtctg gcgctctatg aagagatcaa agagaaagcg ttgagctgga gtctgggccg
    46261 cgcggaaccc cacgaaatcg acgagctgaa cattcttcat gcgaccatgc tggcgatgca
    46321 gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg ttgattgatg gtaaccgctg
    46381 cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggg gatagccgcg taccagaaat
    46441 cagtgccgcg tctatcctag cgaaagtgac gcgtgacgcc gaaatggcgg cgctggatat
    46501 tgttttcccg caatatggtt ttgcccaaca taaagggtat ccaaccgctt ttcatctgga
    46561 aaaactggct gaatatggcg cgactgaaca tcatcgtcgc agctttgggc ctgtcaaacg
    46621 cgcactggga cttgcgtcct gattcttgtg tcgagattaa gtaaaccgga atctgaagat
    46681 gtctgaacca cgtttcgtac acctgcgggt gcacagcgac tactcgatga tcgatggctt
    46741 ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg ggtatgccag cactggcgat
    46801 caccgatttc accaaccttt gcggtctggt gaagttctac ggagcgggac atggcgcagg
    46861 gattaagcct atcgtcgggg cagattttaa tgtccagtgc gacctgctgg gtgatgagtt
    46921 aacccacctg acggtactgg cggcgaacaa taccggctat cagaatctgg cgttgctgat
    46981 ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg atcatcgatc gcgactggct
    47041 tatcgaatta aacgaagggt tgatccttct ttccggcggg cgcatgggcg acgtcggacg
    47101 cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt gtcgcgtttt atgaagaaca
    47161 cttcccggat cgctattttc tagagctgat ccgcaccggc aggccggatg aagaaagcta
    47221 tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg cccgtcgtgg cgactaacga
    47281 tgtgcgcttt atcgacagca gcgactttga cgcacacgaa atccgcgtcg cgatccacga
    47341 cggctttacc ctcgacgatc ctaaacgccc gcgtaactat tcgccgcagc aatatatgcg
    47401 tagcgaagag gagatgtgtg agctgtttgc cgacatccca gaagcccttg ccaacaccgt
    47461 tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt gaatacttcc tgccgcagtt
    47521 cccgaccggg gacatgagca ccgaagatta tctggtcaag cgtgcaaaag aaggcctgga
    47581 agagcgtctg gcatttttat tccctgacga ggaagaacgt cttaaacgcc gcccggaata
    47641 tgacgaacgt ctggagactg aacttcaggt tatcaaccag atgggtttcc cgggctactt
    47701 cctcatcgtt atggaattta tccagtggtc gaaagataac ggcgtaccgg taggaccagg
    47761 ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg aaaatcaccg acctcgatcc
    47821 gctggaattt gacctgctgt tcgaacgttt ccttaacccg gaacgtgtct ccatgcctga
    47881 cttcgacgtt gacttctgta tggagaaacg cgatcaggtt atcgaacacg tggcggacat
    47941 gtacggtcgt gatgcggtat cgcagattat caccttcggt acgatggcgg cgaaagcggt
    48001 gatccgcgac gtaggccgcg tgctggggca tccgtacggc tttgtcgacc gtatctcgaa
    48061 actgatcccg cccgatccgg ggatgacgct ggcgaaagcg tttgaagccg agccgcagct
    48121 gccggaaatc tacgaagcgg atgaagaagt taaggcgctg atcgacatgg cgcgcaaact
    48181 ggaaggggtc acccgtaacg ccggtaagca cgccggtggg gtggttatcg cgccgaccaa
    48241 aattaccgat tttgcgccgc tttattgcga tgaagagggc aaacatccgg tcacccagtt
    48301 tgataaaagc gacgttgaat acgccggact ggtgaagttc gacttccttg gtttgcgtac
    48361 gctcaccatc atcaactggg cgctggagat gatcaacaag cggcgggcga agaatggcga
    48421 gccaccgctg gatatcgccg cgatcccgct ggatgacaag aaaagcttcg acatgctgca
    48481 acgctcggaa accacggcgg tattccagct tgaatcgcgc ggcatgaagg acctgatcaa
    48541 gcgtctgcaa cctgactgct tcgaagatat gatcgcactg gtggcactgt tccgccctgg
    48601 tccgttgcag tcagggatgg tggataactt tatcgaccgt aaacatggtc gtgaagagat
    48661 ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa tcggtactgg agccaaccta
    48721 cggcattatc ttgtatcagg aacaggtcat gcagattgcc caggtgcttt ctggttatac
    48781 cctcggtggc gcggatatgc tgcgccgtgc gatgggtaag aaaaagccgg aagagatggc
    48841 taagcagcgt tctgtatttg ctgaaggtgc agaaaagaac ggaatcaacg ccgaactggc
    48901 gatgaaaatc ttcgacctgg tggagaaatt cgcgggttac ggatttaaca aatcgcactc
    48961 tgcggcctat gctttggtgt catatcaaac gttatggctg aaagcgcact atccggcgga
    49021 gtttatggcg gcggtaatga ccgccgatat ggacaacacc gagaaggtgg tgggcctggt
    49081 ggatgagtgc tggcggatgg ggctgaaaat cctgccacca gatataaact ccggtcttta
    49141 ccatttccac gtcaacgacg acggcgaaat cgtgtatggt attggcgcca tcaaaggggt
    49201 aggtgaaggt ccgattgagg ccatcatcga agcccgtaat aaaggcggct acttccgcga
    49261 actgtttgat ctctgcgccc gtaccgacac caaaaagtta aaccggcggg tgctggaaaa
    49321 actgatcatg tccggggcgt ttgaccgtct tgggccacac cgcgcggcgc tgatgaactc
    49381 gctgggtgat gcgttaaaag cggcagatca acacgcgaaa gcggaagcta tcggtcaggc
    49441 cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt gaacaatcct acgccagctg
    49501 ccaaccgtgg ccggagcagg tggtattaga tggggaacgt gaaacgttag gcctgtacct
    49561 gaccggacac cctatcaacc agtatttaaa agagattgag cgttatgtcg gaggcgtaag
    49621 gctgaaagac atgcacccga cagaacgtgg taaagtcatc acagctgcgg ggctcgttgt
    49681 tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc ggtatctgca cgctggatga
    49741 ccgttccggg cggctggaag tgatgttgtt tactgacgcc ctggataaat accagcaatt
    49801 gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc agctttgatg acttcagcgg
    49861 tgggcttaaa atgaccgctc gcgaagtgat ggatattgac gaagcccggg aaaaatatgc
    49921 tcgcgggctt gctatctcgc tgacggacag gcaaattgat gaccagcttt taaaccgact
    49981 ccgtcagtct ctggaacccc accgctctgg gacaattcca gtacatctct actatcagag
    50041 ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg cgtgtctctc cgagcgatcg
    50101 tttattaaac gatctccgtg gcctcattgg ttcggagcag gtggaactgg agtttgacta
    50161 atacaggaat actatgagtc tgaatttcct tgattttgaa cagccgattg cagagctgga
    50221 agcgaaaatc gattctctga ctgcggttag ccgtcaggat gagaaactgg atattaacat
    50281 cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa ctgacgcgta aaatcttcgc
    50341 cgatctcggt gcatggcaga ttgcgcaact ggcacgccat ccgcagcgtc cttataccct
    50401 agatttcgtt cgcctggcat ttgatgaatt tgacgaactg gctggcgacc gtgcgtatgc
    50461 agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat ggtcgtccgg tgatgatcat
    50521 tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc cgtaactttg gtatgccagc
    50581 gccagaaggt taccgcaaag cactgcgtct gatgcaaatg gctgaacgct ttaagatgcc
    50641 tatcatcacc tttatcgaca ccccgggggc ttatcctggc gtgggcgcag aagagcgtgg
    50701 tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct cgccttggcg taccggtagt
    50761 ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg gcgattggcg tgggcgataa
    50821 agtgaatatg ctgcaataca gcacctattc cgttatctcg ccggaaggtt gtgcgtccat
    50881 tctgtggaag agcgccgaca aagcgccgct ggcggctgaa gcgatgggta tcatcgctcc
    50941 gcgtctgaaa gagctgaaac tgatcgactc catcatcccg gaaccgctgg gcggcgctca
    51001 ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa ctgctggcgg atctggccga
    51061 tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt tatcagcgcc tgatgagcta
    51121 cggttacgcg taattcgcaa aagttctgaa aaagggtcac ttcggtggcc cttttttatc
    51181 gccacggttt gagcaggcta tgattaagga aggattttcc aggaggaaca catgaacatc
    51241 attgccatta tgggaccgca tggcgtcttt tataaagatg agcccatcaa agaactggag
    51301 tcggcgctgg tggcgcaagg ctttcagatt atctggccac aaaacagcgt tgatttgctg
    51361 aaatttatcg aacataaccc tcgaatttgc ggcgtgattt ttgactggga tgagtacagt
    51421 ctcgatttat gcagcgatat caatcaactt aatgaatatc tcccgcttta tgccttcatc
    51481 aacacccact cgacgatgga tgtcagcgtg caggatatgc ggatggcgct ctggtttttt
    51541 gaatatgcgc tggggcaggc ggaagatatc gccattcgta tgcgtcagta caccaacgaa
    51601 tatcttgata acattacgcc gccgttcacg aaagccttgt ttacctacgt caaagagcgg
    51661 aagtacacct tttgtacgcc ggggcatatg ggcggcaccg catatcaaaa aagcccggtt
    51721 ggctgtctgt tttatgattt tttcggcggg aataccctta aggctgatgt ctctatttcg
    51781 gtcaccgagc ttggttcgtt gcttgaccac accgggccac acctggaagc ggaagagtac
    51841 atcgcgcgga cttttggcgc ggaacagagt tatatcgtta ccaacgggac atcgacgtcg
    51901 aacaaaattg tgggtatgta cgccgcgcca tccggcagta cgctgttgat cgaccgcaac
    51961 tgccataaat cactggcgca tctgttgatg atgaacgatg tagtgccagt ctggctgaaa
    52021 ccgacgcgta atgcgttggg gattctgggg gggatcccgc gccgtgaatt tactcgcgac
    52081 agcatcgaag agaaagtcgc tgccactacg caagcacaat ggccggttca tgcggtgatc
    52141 accaactcca cctatgatgg tttgctctac aacaccgact ggatcaaaca gacgctggat
    52201 gtcccgtcga tccacttcga ttccgcctgg gtgccgtata ctcattttca tccaatctac
    52261 cagggtaaaa gtggtatgag cggcgagcgt gttgcgggaa aagttttctt cgaaacgcaa
    52321 tcgacccaca aaatgctggc ggcgttatcg caggcttcac tgatccacat taaaggcgag
    52381 tatgacgaag aggcgtttaa cgaagccttt atgatgcata ccaccacctc gcccagttat
    52441 cccattgttg cttcggttga gacggcggcg gcgatgctgc gtggtaatcc gggcaaacgg
    52501 ctgattaacc gttcggtgga gcgggcgctg cattttcgca aagaggtcca gcggctgcgg
    52561 gaagagtctg acggttggtt tttcgatatc tggcaaccgc cgcaggtgga tgaagccgaa
    52621 tgctggcccg ttgcgcctgg cgaacaatgg cacggcttta acgatgcgga tgccgatcat
    52681 atgtttctcg atccggttaa agtcactatt ttgacaccgg ggatggacga acagggcaat
    52741 atgagtgagg aggggatccc ggcggcgctg gtagcaaaat tcctcgacga acgtgggatc
    52801 gtagtagaga aaaccggccc ttataacctg ctgtttctct ttagtattgg catcgataaa
    52861 accaaagcaa tgggattatt gcgtgggttg acggaattta agcgctctta cgatctcaac
    52921 ctgcggatca aaaatatgct gcccgatctc tatgctgaag atcccgattt ctaccgcaat
    52981 atgcgtattc aggatctggc gcaagggatc cataagctga ttcgtaaaca cgatctttcc
    53041 ggtttgatgt tgcgggcatt cgatactttg ccggaaatga tcatgacgcc acatcaggca
    53101 tggcagagac agattaaagg tgaagtagaa accattgcgc tggaacaact ggtcggtaga
    53161 gtatcggcaa atatgatcct gccttatcca ccgggcgtac cgctgttgat gccaggagaa
    53221 atgctgacca aagagagccg cacggtactc gattttctac tgatgctttg ttccgtaggg
    53281 caacattacc ccggttttga aacggatatt cacggcgcga aacaggacga agacggcgtt
    53341 taccgcgtac gagtcctaaa aatggcggga taacttgcca gagcggcttc tgggcgagta
    53401 acgtgctgtt aacaaataaa ggagacgtta tgctgggttt aaaacaggtt caccatattg
    53461 cgattattgc gacggattat gcggtgagca aagctttcta ctgcgatatt cttggtttca
    53521 cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg gaaaggggat ttggcgctta
    53581 atgggcaata tgtgattgag cttttctctt ttccgttccc gccggagcgc cccagccgac
    53641 cggaagcttg cggcctgcgt catctggctt ttagcgttga tgacatcgat gcggcagtgg
    53701 cgcaccttga aagccataac gtgaagtgtg aagccatccg tgtcgatcca tacacgcaaa
    53761 aacgcttcac cttctttaac gatccggacg ggctgccgtt ggaactgtat gagcagtaag
    53821 gcttgtcatc gccgcatttg cccggtaacg tgccgggcat tgctactgta aaatcgcacc
    53881 atcatgacac tcacgctcaa tagaccactt ctcacctcac gccagattct ggtggccttt
    53941 agcggcgggc ttgactccac cgttctgctg catcagttgg tgcagtggcg gacggaaaat
    54001 ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt taagtgccaa tgccgatgcc
    54061 tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg tgccgctggt ggtcgaacgc
    54121 gtacaacttg cgcaagaggg gctggggatt gaggcccagg cgcggcaggc gcgttatcag
    54181 gcatttgccc gtaccctatt gcccggcgaa gtgctggtca ccgcgcaaca tctcgacgat
    54241 caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg gccctgccgg gctttcggct
    54301 atggcggaag tttcggagtt tgctggaaca cggcttatcc gcccgttgct tgcccgcacg
    54361 cggggggaac tggagcagtg ggcattggcg catggtttac gctggattga agacgaaagt
    54421 aatcaggacg actcatacga tcgtaacttt ctgcgcctgc gcgtagtgcc gttattgcag
    54481 cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg ccacactttg tgctgaacaa
    54541 gagagtctgt tggatgaact gctggcggat gatttagcac actgccaaac gtcgcagggg
    54601 acgctgcaga ttgcgccaat gctggcgatg agtgatgccc gtcgcgcggc gattattcgc
    54661 cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg acgcgctggt gcgcatctgg
    54721 caggaagtgg cgctggcgcg ggaagatgct tccccctgtt tacgtttggg cgcctttgaa
    54781 atccgccgct atcagtcgca actgtggtgg attaaatccg tcaccgggca aagcgaaacc
    54841 attgtgctgt ggcagacgtg gctacaaccg ctggaattac cggcggggct gggaaccgta
    54901 cagcttactg cgggaggcga tattcgccct ccgcgtgcag acgaagcggt cagcgtgcgt
    54961 ttcaaagcgc caggattgct gcatattgtc gggcgcaacg gcggacgtaa gctgaagaaa
    55021 atctggcaag agttaggcgt gccgccgtgg ctacgtgaca ccacgccact gctgttttat
    55081 ggagaaacgc tgattgcggc ggcaggggta tttgtgacgc aagaaggtgt ggctgaaggt
    55141 gagaatggcg taagttttgt ctggcagaaa acgcttagtt aagtgaaagc cggataagac
    55201 gcatcaaacg tcgcatccgg cgaaagtcaa tcaggattcg cttaccacca ccgtaccgat
    55261 ttcagggtgg ctaaagctgg taattttatc cagacgcagc tcgcgggttt cgccagcggc
    55321 ctcgacgacc agatactcca catttttgcg ggagactaaa tcactggctt ttgcctgcaa
    55381 tttttcgcca tctttcagct caagtgtcag cattaaatga tgctggcagg cgagctcaag
    55441 attatcgtaa tcatcacaat taattggttg atacgtatca ttcattgaca taatcgctca
    55501 ccagtaagtt tgccgcagcg tatgctgctt tttccctgac agcctcagaa agggcgtcgt
    55561 cggcagccat ttcattcagc actttcaaaa cgcagcccag cgcgtccgga acgtatccta
    55621 agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa ctcacaatat ttttccacat
    55681 gccctcctgt cagcactctg acttaaccgt ggatgcaagt ctaagcctac gaagataaac
    55741 tctgtttcgc aaggtgacta taccacactc atttctgcaa tatcagcgcc gcaactgcac
    55801 gtattccgtt acaatggcct cctgattcga aaggagtttt cttatggcgc ttaaagcgac
    55861 aatttataaa gcgacggtta atgtggccga tctcgaccgc aaccagtttc tcgatgcctc
    55921 tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt atgatgctgc gcttgctggc
    55981 gtggctgaaa tatgctgatg aacgtctgca atttacccgt ggtttgtgtg ccgatgatga
    56041 gccggaagcg tggctgcgta acgatcacct gggcattgat ttgtggattg agctggggct
    56101 gccggatgag cgacgaatta agaaagcctg cacccaggcc gcagaagtgg cgctgtttac
    56161 ctataatagt cgggcggcgc aaatctggtg gcagcaaaat cagagcaaat gtgtgcagtt
    56221 tgccaatctt tccgtctggt atctggacga tgaacaactg gcgaaagtaa gcgcctttgc
    56281 cgatcgtacc atgacgctgc aggcaacgat tcaggatggt gttatctggt tatcggatga
    56341 taagaataat ctggaagtta acttaaccgc ctggtaacaa ccttcatgat tgtgatttcc
    56401 cgacatgttg ctattcccga tggtgagctt gagatcaccg ccattcgtgc gcagggcgct
    56461 ggcgggcagc atgttaataa gacctcaacg gctattcatc tgcgttttga cattcgggcg
    56521 cccagcctgc cagagtatta caaagagcgt ctgctcgccg ccagccatca tttgatcagc
    56581 agtgatggcg tgattgtcat taaggcacag gaataccgca gtcaggaact gaaccgcgaa
    56641 gccgctctgg cccggttggt ggcagtgatt aaagatttaa caaaagaaca aaaagcccga
    56701 cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa
    56761 tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc gggaataaaa agaaggaatg
    56821 gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc agcctcttta ctctgatggg
    56881 atgtaataat cgggccgaag tcgatacgct ttctccggcg caggctgccg aactgaaacc
    56941 gatgccgcaa agttggcgcg gcgtgctacc gtgtgccgat tgcgaaggaa tcgaaatctc
    57001 tctgttcctc gaaaaagacg gaacatgggt gatgaatgag cgttatctcg gtgctcgtga
    57061 agaaccttcc tccttcgctt cctacggtac atgggcgcga accgctgaca agctggtatt
    57121 aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct
    57181 cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat acgctggaac cggcacaatc
    57241 cagtttacct atgacgccga tgaccctgcg aggcatgtat ttttatatgg ctgatgcggc
    57301 gaccttcact gattgcgcga ccggaaaacg tttcatggta gcgaataacg cagagctgga
    57361 gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg gtgttactgt cagtagaagg
    57421 tcactttacg cttgaggcta atccggatac tggtgcgcca accaaagtat tagcgcccga
    57481 tacggcgggt aaattttacc cgaataagga ttgcagtagt ttggggcagt aaacctgctt
    57541 aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct tatcaggcct acagcatata
    57601 atgcaacatt ttgaattagc acgattttgt aggccggata aggcgtttac gccgcatccg
    57661 gcattttgtg cacgaggctg gctttatcac tgagtcagcc caacaaagcg acccgccttg
    57721 atataaatcc caggcttcag gtgcccaaca tactgtaact gcgtttctgg cttaaacgca
    57781 gagacatcac caatacgcat atgcatcaga tcggcagggc taatccagcc cgattgcgcc
    57841 agtgtaagtg gatgcccggc tttggcaaat gcatcagtga caaattccga acaaaaccac
    57901 gactttttgt ctccttcgca cacactgctt aactgcgctt tcgccaggtc gctgacgcac
    57961 tgttggcgaa aatcctcgga gaacggattc agtgagcaca tctggcgagt caccataaag
    58021 ggaataaatt cgacaatgtt gcgatagtta taaccgctat ctttgatttt attggcaaac
    58081 gcggtgatat ctgtggcttg ttgcggggta agatccggga ctcgtaagac gaaaagctta
    58141 tcactatgct tcatcgcttt tttaagggaa acaatctgga cgccagcgcc tgtcgcttct
    58201 gcaacgttat tatcacccag aaagattgca acgtgactca cagaggaagt gctgaagacg
    58261 cggattccaa atgaggttac cccaaggctt gaggagaaca gcaaatcgcc gggtttgaga
    58321 tcaggtgccg ttatttcttt tattgattgt tcggtgaaag agctttgatg ctggaatttg
    58381 acagcccatg tttttgcctc ggcatctact gctgtggctg atgggtctgg ctggctgata
    58441 tcaacggtac aagccgaaag taaaagaaag ctggggagaa gcaggcggca gtacgccttt
    58501 ggtttatcca ttttatacaa tccatgtaaa aaagggccct gaaattcagg accctttctg
    58561 gcatcagcct ttaatctgtt tcaccagata ttcgacgatg tcaccagtct taattaactg
    58621 tttctcgccg ttgcgacgat atttatattc gatatcgtcg ttgtcgaggt tacggtcgcc
    58681 cagcacaata gtgtgcggaa taccgatcag ttccatatca gcaaacatca cgcccggacg
    58741 ctctttgcgg tcatccagca gcacttcgat accttgtgca cgcagttcgc tgtacagttt
    58801 ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc atcggcagaa tcgccacctg
    58861 gaacggcgcg atagcgtcag gccatacgat gccgcgttcg tcgtagttct gctcaatcgc
    58921 cgcagctacc acacgcgtta ccccgatacc gtagcaaccc atcgtcagga tttggttacg
    58981 gccatcttca ccctgtacgg aggctttcag tgcttcggag tacttggtac ccagctggaa
    59041 gatgtgacca acttcgatac cacgtttgat cagcagcgta ccctggccat ccgggcttgg
    59101 atcgccagcc accacgttac ggatatctgc aacttccggg gtagcgacat cgcgatccca
    59161 gttgataccg aagtagtgtt taccatcgat gttagcacca gcagcgaaat cactcatcgc
    59221 cgcaacggta cggtcaatca ccaccggaat cggcatgttt accggaccca gtgaacccgg
    59281 accggcttta accacagcac gaatttcttc ttcggtcgcg aaagtcagcg ggcttgcaac
    59341 ctgcggcagt ttttctgctt taacttcgtt cagctcgtga tcaccgcgca ccagcagcgc
    59401 aaccagcggg aagctactgc cttcaaccgc tttaaccagc agagtcttaa ccgttttctc
    59461 aatcggcaga ttgaactgtt caaccagttc cgcgatggtt ttcgcgttcg gcgtatcaac
    59521 cagcgtcatt tcctgggtag cagcagcgcg cggttctttc ggcgcgatag cttctgccag
    59581 ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc acatcgtctt caccgctctg
    59641 cgccagcacc tggaattcgt gagaggcgct gccgccgata gaaccggtgt cggcctgtac
    59701 ggcgcggaaa tccagcccca tgcggctgaa gattttgctg taggccgcat acattgcatc
    59761 gtaggtttcc tgcagggatt cctgagaagt atggaaagag taagcatctt tcatcaggaa
    59821 ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg tcgcggaact tggtctggat
    59881 ctgatagaag ttcagcggca gctgtttgta agagctaagc tcgttacgaa tcaggtcagt
    59941 gataacttct tcatgagttg ggccgagtac gaacggacgc tcgccacggt caacaaaacg
    60001 cagcagttcc ggaccgtact gttcccaacg accactctct tgccacaaat cggctggctg
    60061 aaccaccggc atcgacacct cgatcgcacc ggcgttgttc atctcttcac gcacgatgtt
    60121 ttcgactttt ttcagaacgc gcacgccggt cggcagccag gtatataacc cggaggccag
    60181 cttgcggatc atcccggcgc gcagcatcag ctgatggctg atcacctcgg cgtcggcagg
    60241 tgtctccttg agagtggaga gcaggtattg gctagtacgc atgttgttac ggttccagtt
    60301 ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa agtgatttag tttaccagtg
    60361 caaaagaaat gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca
    60421 aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac ggcataggtt
    60481 ttgcccgttt cctcaccttt acgataggcc gggcgcgggt cctgcgccag tacttcgcgg
    60541 ataaacagcg ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct
    60601 gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt gcgcatagct ggcactggca
    60661 tcgggaagcg attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg
    60721 accagatcca gactgccgag cttcagaatc acgctgtctt tatggcaaac aacctctttc
    60781 agctctacca gcgacatgcc aattgggtta gggcggaaag tagagcgtgt tgcgaaaacc
    60841 cccattctgg cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca gccgccttcc
    60901 atcgtttgat gaaagacgaa aaggatccat aaatggctga atgcttccag gccgcgaacg
    60961 gcgtcggcct ggttgtagga agcaatgaga tgcagttcgc cgttggcgct ttttaccaga
    61021 cctggctggc gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc
    61081 tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc agagccgata catacagcct
    61141 gacgatagca gcccggcgta ccgctggtga cttcgcagct atgcagtaat acagcattgg
    61201 ctttcatttt agaggcattg atttgcatct gcttacgtgc ggttggaatg ctcggcggag
    61261 agtcctgatt agaggcctgg caagagtcgc cactgacttc accgagatcg cggaacggtt
    61321 tgccgactaa ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg
    61381 gttttgcagg ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc
    61441 tgcttaacat ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca
    61501 tagtatttcc tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga
    61561 caagacgggc aagcgcccgt cctgaatgat attacaaatt gtggaaacag cctaaaaatt
    61621 accagccttt aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcatcag
    61681 actgataagc ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta
    61741 cgatcaggtt tacgtatggg gagtctttat cttcgacaaa gataccgtct ttcgccggag
    61801 tcaggccaat ctggctggca taggtggtat tgataactgc cagagcgatt tgcgcgtcgt
    61861 ccagagaacg cggcagttgc ggtgcttcca gttcaacaat tttcagattt tttgggttct
    61921 caacaacatc aagaactgtc ggcagcaggc caacgccatc tttcagtttg atcaagccca
    61981 ctttttgcag cagcagcagt gaacgaccaa gattagttgg atcgtttggc acggcaacct
    62041 gcgaaccatc ctgcagttca tccagtgatt tgattttctt ggagtaacca gcaatcggat
    62101 aaacaaaagt gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa
    62161 ggtacggttt atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg
    62221 gcagaacata gtcgttgaag gttaccagct caacgtccag gccatatttg tctttcgcaa
    62281 ctttctgcgc gacttctgct acctgctgtt cggcaccaac aatcacgccg actttaatgt
    62341 ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc
    62401 ccactgccgc aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat
    62461 gttgtgttga acgttacttg cgagtgacag cccggacgat gcggtcgcct gcgaactgaa
    62521 ttaaataaac cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttgtagc
    62581 cgatgtagcc atactgatag ccaatctgac ctaaaccacc ggcaccgact gcaccaccca
    62641 tcgcggaata accgaccagg gtaatcaggg tgatagttgc cgcattcacc agacccggca
    62701 acgcttccgg taacagcact ttacggacga tctgcattgg cgtggcaccc attgcgcggg
    62761 aagcttcaat taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa
    62821 acggtgctgc accaacggtt aacggaacaa ttgctgcctg caaaccaatc gatgtaccga
    62881 caataacgcg ggtaaacgga atcatccata ccagcaagat aatgaacggg atggaacgga
    62941 aaatgttcac aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg
    63001 gacgcgtgac ataaagcaga acgccaaccg gcagaccaat cacaaaccca aaaaatccgg
    63061 atacgaaggt cattgccagc gtttcccata cgccacgaac cagcagccac atcatcggct
    63121 cagacataac ccagtacctc tacttttaca tggtgttcct gcagccaggc aatggcggct
    63181 tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga tgccgaattt aacgccaccg
    63241 gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca
    63301 gaaagcagtg gggcatcgac cgattgaccg gtaaactcca gacgcagcat cggcacgcag
    63361 tcagtaaatg gctccgtttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg
    63421 gtcgactgaa taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc
    63481 gtgtcctgct cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc
    63541 acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttaatgtct
    63601 ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca
    63661 cacagcaata ctttgggatt gctggctaat gcacgggcaa tcgccacacg ttgtttctgc
    63721 ccaccggaaa gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc
    63781 aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc cagcggcaga
    63841 gccacgttgc caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca
    63901 atctggcggc gagctttggt caactcggat tctgacagcg tggtcaattc ctggccatcg
    63961 accagcacgc taccctcggt tgggcgctcc agcaggttta cacaacgtat aagcgtactc
    64021 ttacccgcgc ctgaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg
    64081 acgttgttca acgcctggat ggtgcgggtg ccctggtgga acactttggt gatattcgaa
    64141 agttttatca ttgattattt attatcgtca ttaagttagt cgtagcatct cgaatgcctg
    64201 aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag
    64261 gtttatgcga agagcacttt cttgcaggtc gaaacatgcg atactagcgt cccatgcctt
    64321 attaaggagc tataaaaggt ggcgaagagc gtacccgcaa tttttcttga ccgtgatggc
    64381 accattaatg tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt
    64441 gttattgacg ccatgcgcga gctaaaaaaa atgggctttg cgctggtggt agtaaccaac
    64501 cagtctggca ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct gaccgagtgg
    64561 atggactggt cgctggcgga ccgagatgtc gatctggatg gtatctatta ttgcccgcat
    64621 catccgcagg ggagtgttga agagtttcgc caggtctgcg attgccgcaa accacatccg
    64681 gggatgcttt tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg
    64741 ggcgataaat tagaagatat gcaggcagcg gttgcggcga acgtgggaac aaaagtgctg
    64801 gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg cggcagattg ggtgttaaat
    64861 agcctggcag acctgccgca agcgataaaa aagcagcaaa aaccggcgca atgattaaaa
    64921 gatgaacggt tgaaataaaa atgcattttt ccgcttgtct tcctgagccg actccctata
    64981 atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga
    65041 agagaaaaaa tcctgaaatt caggggtgac tctgaaagag gaaagtgtga tatacgccac
    65101 ctcgcgacag tgagctgaaa gc
//
LOCUS       DAJTYI010000007        60518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYI010000007 DAJTYI010000000
VERSION     DAJTYI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..175
                     /locus_tag="OO564_000463"
     CDS             <1..175
                     /locus_tag="OO564_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCR6651718.1"
                     /translation="KDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAG
                     EFKATSTFQVTYP"
     gene            288..1082
                     /gene="panB"
                     /locus_tag="OO564_000464"
     CDS             288..1082
                     /gene="panB"
                     /locus_tag="OO564_000464"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6651719.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1094..1945
                     /gene="panC"
                     /locus_tag="OO564_000465"
     CDS             1094..1945
                     /gene="panC"
                     /locus_tag="OO564_000465"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6651720.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2019..2921)
                     /gene="rpnC"
                     /locus_tag="OO564_000466"
     CDS             complement(2019..2921)
                     /gene="rpnC"
                     /locus_tag="OO564_000466"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6651721.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3195..3575
                     /gene="panD"
                     /locus_tag="OO564_000467"
     CDS             3195..3575
                     /gene="panD"
                     /locus_tag="OO564_000467"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6651722.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3579..4808)
                     /locus_tag="OO564_000468"
     CDS             complement(3579..4808)
                     /locus_tag="OO564_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6651723.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4872..5312)
                     /locus_tag="OO564_000469"
     CDS             complement(4872..5312)
                     /locus_tag="OO564_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6651724.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5417..6187)
                     /locus_tag="OO564_000470"
     CDS             complement(5417..6187)
                     /locus_tag="OO564_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6651725.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6184..7110)
                     /locus_tag="OO564_000471"
     CDS             complement(6184..7110)
                     /locus_tag="OO564_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6651726.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7219..7881
                     /gene="can"
                     /locus_tag="OO564_000472"
     CDS             7219..7881
                     /gene="can"
                     /locus_tag="OO564_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6651727.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7922..8458)
                     /gene="hpt"
                     /locus_tag="OO564_000473"
     CDS             complement(7922..8458)
                     /gene="hpt"
                     /locus_tag="OO564_000473"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6651728.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8664..11054
                     /gene="gcd"
                     /locus_tag="OO564_000474"
     CDS             8664..11054
                     /gene="gcd"
                     /locus_tag="OO564_000474"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6651729.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11101..12650)
                     /gene="cueO"
                     /locus_tag="OO564_000475"
                     /pseudo
     CDS             complement(11101..12650)
                     /gene="cueO"
                     /locus_tag="OO564_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12816..13163
                     /locus_tag="OO564_000476"
     CDS             12816..13163
                     /locus_tag="OO564_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6651730.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13269..14135
                     /gene="speE"
                     /locus_tag="OO564_000477"
     CDS             13269..14135
                     /gene="speE"
                     /locus_tag="OO564_000477"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6651731.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14151..14945
                     /gene="speD"
                     /locus_tag="OO564_000478"
     CDS             14151..14945
                     /gene="speD"
                     /locus_tag="OO564_000478"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6651732.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14983..15345)
                     /locus_tag="OO564_000479"
     CDS             complement(14983..15345)
                     /locus_tag="OO564_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6651733.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15521..18118)
                     /gene="acnB"
                     /locus_tag="OO564_000480"
     CDS             complement(15521..18118)
                     /gene="acnB"
                     /locus_tag="OO564_000480"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6651734.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18473..20326
                     /locus_tag="OO564_000481"
     CDS             18473..20326
                     /locus_tag="OO564_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6651735.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20397..21827)
                     /gene="lpdA"
                     /locus_tag="OO564_000482"
     CDS             complement(20397..21827)
                     /gene="lpdA"
                     /locus_tag="OO564_000482"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6651736.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22152..24044)
                     /gene="aceF"
                     /locus_tag="OO564_000483"
     CDS             complement(22152..24044)
                     /gene="aceF"
                     /locus_tag="OO564_000483"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6651737.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24059..26722)
                     /gene="aceE"
                     /locus_tag="OO564_000484"
     CDS             complement(24059..26722)
                     /gene="aceE"
                     /locus_tag="OO564_000484"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6651738.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26883..27647)
                     /gene="pdhR"
                     /locus_tag="OO564_000485"
     CDS             complement(26883..27647)
                     /gene="pdhR"
                     /locus_tag="OO564_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6651739.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28188..29561
                     /gene="aroP"
                     /locus_tag="OO564_000486"
     CDS             28188..29561
                     /gene="aroP"
                     /locus_tag="OO564_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6651740.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29604..30458)
                     /gene="ampE"
                     /locus_tag="OO564_000487"
     CDS             complement(29604..30458)
                     /gene="ampE"
                     /locus_tag="OO564_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6651741.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30455..31006)
                     /gene="ampD"
                     /locus_tag="OO564_000488"
     CDS             complement(30455..31006)
                     /gene="ampD"
                     /locus_tag="OO564_000488"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6651742.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31094..31987
                     /gene="nadC"
                     /locus_tag="OO564_000489"
     CDS             31094..31987
                     /gene="nadC"
                     /locus_tag="OO564_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6651743.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32190..32630
                     /gene="ppdD"
                     /locus_tag="OO564_000490"
     CDS             32190..32630
                     /gene="ppdD"
                     /locus_tag="OO564_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6651744.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32640..34025
                     /gene="gspE"
                     /locus_tag="OO564_000491"
     CDS             32640..34025
                     /gene="gspE"
                     /locus_tag="OO564_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR6651745.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34015..35217
                     /gene="hofC"
                     /locus_tag="OO564_000492"
     CDS             34015..35217
                     /gene="hofC"
                     /locus_tag="OO564_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6651746.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35252..36295)
                     /gene="guaC"
                     /locus_tag="OO564_000493"
     CDS             complement(35252..36295)
                     /gene="guaC"
                     /locus_tag="OO564_000493"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6651747.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36457..36495)
                     /locus_tag="OO564_000494"
     CDS             complement(36457..36495)
                     /locus_tag="OO564_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651748.1"
                     /translation="MIIKTLKMSARK"
     gene            36520..37140
                     /gene="coaE"
                     /locus_tag="OO564_000495"
     CDS             36520..37140
                     /gene="coaE"
                     /locus_tag="OO564_000495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6651749.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37140..37883
                     /gene="zapD"
                     /locus_tag="OO564_000496"
     CDS             37140..37883
                     /gene="zapD"
                     /locus_tag="OO564_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6651750.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37893..38090
                     /gene="yacG"
                     /locus_tag="OO564_000497"
     CDS             37893..38090
                     /gene="yacG"
                     /locus_tag="OO564_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6651751.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38190..38579)
                     /gene="mutT"
                     /locus_tag="OO564_000498"
     CDS             complement(38190..38579)
                     /gene="mutT"
                     /locus_tag="OO564_000498"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6651752.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38639..41344)
                     /gene="secA"
                     /locus_tag="OO564_000499"
     CDS             complement(38639..41344)
                     /gene="secA"
                     /locus_tag="OO564_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6651753.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41406..41918)
                     /gene="secM"
                     /locus_tag="OO564_000500"
     CDS             complement(41406..41918)
                     /gene="secM"
                     /locus_tag="OO564_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6651754.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42149..43066)
                     /gene="lpxC"
                     /locus_tag="OO564_000501"
     CDS             complement(42149..43066)
                     /gene="lpxC"
                     /locus_tag="OO564_000501"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6651755.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43167..44318)
                     /gene="ftsZ"
                     /locus_tag="OO564_000502"
     CDS             complement(43167..44318)
                     /gene="ftsZ"
                     /locus_tag="OO564_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6651756.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44379..45641)
                     /gene="ftsA"
                     /locus_tag="OO564_000503"
     CDS             complement(44379..45641)
                     /gene="ftsA"
                     /locus_tag="OO564_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6651757.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45638..46468)
                     /gene="ftsQ"
                     /locus_tag="OO564_000504"
     CDS             complement(45638..46468)
                     /gene="ftsQ"
                     /locus_tag="OO564_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6651758.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46470..47390)
                     /gene="ddlB"
                     /locus_tag="OO564_000505"
     CDS             complement(46470..47390)
                     /gene="ddlB"
                     /locus_tag="OO564_000505"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6651759.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKSLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47383..48858)
                     /gene="murC"
                     /locus_tag="OO564_000506"
     CDS             complement(47383..48858)
                     /gene="murC"
                     /locus_tag="OO564_000506"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6651760.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48912..49979)
                     /gene="murG"
                     /locus_tag="OO564_000507"
     CDS             complement(48912..49979)
                     /gene="murG"
                     /locus_tag="OO564_000507"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6651761.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49976..51220)
                     /gene="ftsW"
                     /locus_tag="OO564_000508"
     CDS             complement(49976..51220)
                     /gene="ftsW"
                     /locus_tag="OO564_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6651762.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51220..52536)
                     /gene="murD"
                     /locus_tag="OO564_000509"
     CDS             complement(51220..52536)
                     /gene="murD"
                     /locus_tag="OO564_000509"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6651763.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52539..53621)
                     /gene="mraY"
                     /locus_tag="OO564_000510"
     CDS             complement(52539..53621)
                     /gene="mraY"
                     /locus_tag="OO564_000510"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6651764.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53615..54973)
                     /gene="murF"
                     /locus_tag="OO564_000511"
     CDS             complement(53615..54973)
                     /gene="murF"
                     /locus_tag="OO564_000511"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6651765.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54970..56457)
                     /gene="murE"
                     /locus_tag="OO564_000512"
     CDS             complement(54970..56457)
                     /gene="murE"
                     /locus_tag="OO564_000512"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6651766.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56444..58210)
                     /gene="ftsI"
                     /locus_tag="OO564_000513"
     CDS             complement(56444..58210)
                     /gene="ftsI"
                     /locus_tag="OO564_000513"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6651767.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58226..58591)
                     /gene="ftsL"
                     /locus_tag="OO564_000514"
     CDS             complement(58226..58591)
                     /gene="ftsL"
                     /locus_tag="OO564_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6651768.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58588..59529)
                     /gene="rsmH"
                     /locus_tag="OO564_000515"
     CDS             complement(58588..59529)
                     /gene="rsmH"
                     /locus_tag="OO564_000515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6651769.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59531..59989)
                     /gene="mraZ"
                     /locus_tag="OO564_000516"
     CDS             complement(59531..59989)
                     /gene="mraZ"
                     /locus_tag="OO564_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6651770.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14479 a        16772 c        14886 g        14381 t
ORIGIN      
        1 taaagattac gacccgagag gcaaagacga tacaacagga ggagtttacc cggatcaaga
       61 tacaggtata acataccctc tccatttcca ggccacgcta caacaggatg gaactatacc
      121 aatagaagct ggtgagttta aagccaccag tactttccag gtaacctacc cttaataagt
      181 ccaccgcacc gccatcatct ggcggtgcgg taattgataa atcattgccc ccaccaatgt
      241 gactgataaa atgccaggca acacgacatc atttatcagg atacgttatg aaaccgacca
      301 ccattgcttc acttcagaaa tataagcagg ataaaaagcg tttcgcgacc atcaccgctt
      361 acgactacag cttcgcgaaa ctatttgctg atgaagggct taacgtcatg ctggtaggcg
      421 actcgctggg catgaccgtt cagggccacg attccactct gccagtaacc gttgctgata
      481 tcgcctacca cactgccgcc gtacgtcgcg gcgcaccaaa ctgcctgcta ttggctgacc
      541 tgccgtttat ggcgtatgcc acgccggaac aagcctttga aaacgccgca acggttatgc
      601 gtgccggtgc caatatggtc aaaattgagg gcggtgagtg gctggtcgaa accgtaaaaa
      661 tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg tttaacccca cagtcagtga
      721 atattttcgg tggctacaaa gtacaggggc gcggcaatga agcgagcgat cgactactca
      781 gcgatgcatt agccttagaa gccgcggggg cacagctgct ggtgctggaa tgcgtgccgg
      841 ttgagctggc aaaacgtatt accgaagcac tggcgatccc ggttattggt attggtgcag
      901 gcaacgtcac tgacgggcaa atcctcgtga tgcacgacgc ctttggtatt accggcggtc
      961 acattcctaa atttgctaaa aatttcctcg ccgaaacggg cgacatccgc gcggctgtgc
     1021 ggcagtatat ggctgaagtg gagtccggcg tttatccggg cgaagaacac agtttccatt
     1081 aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg ctgcgtcagc aaattcgccg
     1141 cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact atgggtaatc tgcacaatgg
     1201 ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat gtggtcgtcg tcagtatttt
     1261 cgttaacccg atgcagttcg accgcccgga agatctggca cgttatccac ggaccttgca
     1321 ggaggactgc gagaagctaa acaaacgtaa agtggattta gttttcgccc cttcggtaaa
     1381 agagatctac ccgaacggta ctgaaaccca cacttacgtt gacgttcctg gcctttcaac
     1441 catgctggaa ggtgccagcc gtccgggaca ttttcgcggc gtttcgacta ttgtcagcac
     1501 gctgttcaac ctggtccagc cggacatcgc ctgcttcggt gaaaaagatt ttcagcaact
     1561 ggcgttgatc cgcaaaatgg ttgccgatat gggcttcgat attgagattg tcggtgtgcc
     1621 aattatgcgc gccaaagacg gtctggcgct aagttcccgt aacggttatc tgacggcgga
     1681 acaacgcaaa attgcgcctg gtctgtacaa agttttaagt tcgattgctg acaaattgca
     1741 ggctggggaa cgggatctcg atgaaattat taccattgcg gggcaagaac tgaatgaaaa
     1801 aggcttccgc gccgatgata ttcagattcg cgatgccgac acattgctgg aagtttctga
     1861 aaccagcaaa cgggcagtaa ttctggtagc cgcctggctt ggcgatgctc gcctgatcga
     1921 caacaaaatg gtcgagctgg cgtaatactt aactggcgct acggctgatg gcgccagtta
     1981 ttaatttacc ccacgtcatc tgtcataaca caggtaactt aaataaggtt aattacctta
     2041 tcaatctcag caagaggtaa atttgccatc tctgcaacgt cttcccgaga cattccttta
     2101 cttagaagac gcagggcgaa ttcttgcctt tcctcctgtc ttccctgcgg aatccccttc
     2161 tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca tagacttccc tcccgtttcc
     2221 ctgtctctca gcacaccata aaacaaatcc gcttgttcag tatgaccgcg ttgcagcata
     2281 tagttttgca tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat cagcgtgacc
     2341 agttgctcca gcaataacat taagtcgcgc tggcgaatat gtttttgcag tagttcgaga
     2401 atcgcaatcc gccgatgttg catgatttcg tcatccggtg tgatggtgat atccaccagc
     2461 gggaaaggac tgttatagac gcgtcgcgcc agctccggcg agtaaaacat atcaaaccag
     2521 cacattgaga gcggataagg tgtggcctcg ccctgataaa acagtatcgg caccaccagc
     2581 ggcagcttat cgtggccagc ctccagatgc cggtgcatgg cagcaataga ataacgcatc
     2641 atgcgaaagg ccattttctt atccggcttg ctttggtgtt caatcacaac atgcagataa
     2701 ccgggattgc cctgcatttg cacggaatag agcacgtccg tgctgtgacc tttcaggctc
     2761 tcttcaatga aactccccga ctctaaatga agcgtgttga gatcacaaag ttcgcgtaat
     2821 tccactggca aatgtatctc cagaaagtcg cgagccgtct ccgcatgcat taaaaattgt
     2881 ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca tcttccctgt cttccgtgtg
     2941 gccttgcgtg aaggcagtat ccagtcaagg cgtgctatcg tcagccacct ctttattgtt
     3001 taccgagcag cattcataaa aatatcaatt cgttacaggc gttacatggc acgcttcggc
     3061 gcggtacgca aactgctgac gcaaatgcca tcagatgagt agacactcat caaaaatcgg
     3121 gcaatactgc gtgagaattt cctcaaagca ggcaccgcct gcttcgttaa cgacagggta
     3181 gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc gtgaaagtga
     3241 ctcatgcgga cctgcactat gaaggttctt gcgccattga ccaggatttt cttgacgcag
     3301 ccggtattct cgaaaacgaa gccatcgata tctggaatgt caccaacggc aagcgtttct
     3361 ccacttatgc catcgcggca gaacgcggtt cgagaattat ttctgttaac ggtgcggcgg
     3421 cccactgcgc cagcgtcggc gatattgtca tcatcgccag cttcgttacc atgccagatg
     3481 aagaagctcg cacctggcga cccaatgtcg cctattttga aggcgacaat gaaatgaaac
     3541 gtaccgcgaa agcgattccg gtacaggttg cttgattgtt atccctgcgg ctggttactc
     3601 accagccgcg acatcttctc cagcgaatcc gttcttaaga tataaagtcg ttttagtaac
     3661 aacggattat cccccggttt tactttgcct ttcatggttg tcaccgccag gtgaaatccg
     3721 gcatcgtttg ctgccttcac ggctttgtca ttaaagccgc caaacggata cgaaagatac
     3781 cagacatgtg gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc aaacagaata
     3841 ttgtgctcac tacggctcag taatatgggt cggcgataac catctacccg atgcaaaaaa
     3901 tgggtatgtg actggaaatc aaaaacatcg cgaatttcgt taagctcaga aacgctcata
     3961 aattgcagcg attttgggtt ccacttctgc gggtgacgtt tgatgcgtga ggtaacaata
     4021 aacaccgtcg ccttcatgcc atattgtttc aacacaggat acgcatagcg gctcaccgac
     4081 ttgaggccat catcaaaggt aatcaccacc gctcgcgcag ggagattgat cgtattcttc
     4141 acgtaacctt ccagctgagc catgctcagt gtcgcgtatc ccctgtcgcg cagccaggcc
     4201 atttggttat tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa acgggtgttt
     4261 tcttcgtcgc gcagaatatg gtgataagtt agcaccggca ggccattatc gggttgggca
     4321 tccagcgcgc tgatataggc cagtcgatcg ccaatacgga tctgatacca ggtttgattt
     4381 aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag taccccaaat
     4441 ggcgcactgc ccacactcgg cgcgttgtag accggcgtat ctttccaggt aattaagttc
     4501 tgattactca gcggcttgtt aaggtcaccc aaaccgtctt caactttttg tcgcccctga
     4561 actggctcga gatgcccttt atcgataaac cctttaccaa agccaaaatt aaatgcgtaa
     4621 taacttgcgg cagtgggctc tacggcaatg atttgtccgg cccgaatatt ccccacggtc
     4681 accatcttgt caccaatttg cgcccagacc gcagcatttt cgatggtttg catataacgg
     4741 gcaggtaacg cggcactgac acttgcggta aacagcatca gcaggagaat aacagcttgt
     4801 ttatacatga aaaaataacc gagggtgaaa caacgctttt attgtagcaa aagcgccatc
     4861 agaatcgcgg gttaatactc atacaaatta tgtttgagga cgaaaggcgg atttttttgt
     4921 tgttggtgcc aaagactact cacctccggc ccacccagtt cgacaataca ctcacgaaac
     4981 tctgaactgg tcatggtttc acggcaagcc atcatctgtt caattaacgg taacgtgaca
     5041 ccagaaatca cttcgcaacg ggagtgtttg tggcttaata atgaagccac gcgatacggt
     5101 ggcgctccgg ctatatcggt taagaagatg acaccctcac cagagtccgc ttcatgcaga
     5161 gcatcgcaca tcatgcggct gagcatatta gagcttaatc cgcgccagaa attcacggcc
     5221 cggcactgaa gaagtgcccc atgttttttc tccagcgcat ccagtatctc ttgcgcccgg
     5281 tcatcgtgac aggtaattac ccaacctaac atcgccttcc tccttagccg aaaatcagcc
     5341 taccctgacc attgttgata aagggtgata gccgtcaaaa ctcctctccc ccgaatccag
     5401 aggagaggaa atagccttag ctacgcaaac cacgtccacg ttggatcagc gaccaacaga
     5461 tcaaataaaa cgccacaata aagaccacca gtacgccaaa ggtagtgacc agcggcacat
     5521 catttatacc gaggaagccg tagcggaaac cactgatcat ataaacgatt gggttcaggt
     5581 gcgacagccc ttgccagaac ggcggcaaca aagtcagtga gtaaaagacc ccgcccaaat
     5641 acgtgagtgg cgttaacaca aaggttggca ccaggctgat gtcatcgaac gttttggcaa
     5701 acacgccgtt cagcaaaccc gcaagggaga atagcaccgc cgtgagcacc agggttaagg
     5761 caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt gccgtcacca
     5821 gaatgccaac aaacagacca cgcgccacgc caccgccgac atatccggca ataatgacgt
     5881 gagtcggaac cggcgctacc agcagctctt caatattacg ctggaacttg gcaccaaaaa
     5941 atgatgacgc gacgttagcg taggcgttgg tgatcaccga catcatgatc agccccggta
     6001 cgatgaactg catatagctg aagccatgca tatcgccaat acgcgaacct atcaggttac
     6061 cgaagataat aaagtaaagg gtcatggtga tgactggcgg caccagcgtc tgcacccaga
     6121 tacgcataaa gcgatggatc tctttcgccc agatgctttt tagcgccacc cagtaaagat
     6181 gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa acaaacagct cttccagacg
     6241 gttagctttg ttacgcatac ttaatacctg aatgccctgc tcacttaact gcgtaaatac
     6301 gctgttgatc ccctgctcac gtagcacttc aacttccagc gtcgcagtat cgaccagtcg
     6361 atactgatag ccatcgagct tcggtaacgg acttttcggt gcgagatcga gaataaaggt
     6421 ttccgatttc agcttcgcca gcagcgcctt catcgaggta ttttccacca gctcaccatg
     6481 ttgaataatg ccgatattga ggcacagcat ttctgcttct tccaggtagt gtgtggtaag
     6541 aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg agcggcgaag
     6601 ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt catgcattaa
     6661 cgcacgggca atcattaaac ggcgcttcat cccgccagat aacatacgcg cacgttcgtt
     6721 gcgttttccc cataaatcga gctgcttaag atatttttcg ctgcggatgt acgcctcttt
     6781 gcgctccaca ccgtagtacc ctgcctgatt caccacaatt tgctgcacgg tttcaaacgg
     6841 gttgaagtta aattcctgcg gcaccagtcc caactgacgt ttagcgttca cgacatcttt
     6901 ctcgagatcg taaccaaata cgctgacccg cccggaggtt ttatttacca gagagctgat
     6961 aataccgata gtggtcgatt tcccggcccc gttcggcccg agaagcgcat aaaaatcacc
     7021 cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg ccgcctggat agtttttttt
     7081 aagctgttga agttccagtg caatggtcat aaatttttac ttaccttacg ttcttacact
     7141 ttatctgtgg tttaaatcgt cccggagttg ccctatatta gccaaacgta attatttggt
     7201 tacaggtcgt taacctccat gaaagacata gatacactca tcagcaacaa tgcactatgg
     7261 tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga aactggcaca agcgcaaaaa
     7321 ccgcgctttc tatggattgg atgttccgac agtcgcgttc ctgcagaacg tttaaccggt
     7381 cttgagccgg gcgaactctt tgttcaccgt aatgttgcta acctggtcat tcacaccgac
     7441 ctgaactgcc tttccgtggt tcagtatgca gtggatgtac tcgaagttga acacattatt
     7501 atctgtggcc actacggttg cggcggcgta caagccgccg ttgaaaaccc ggaactgggg
     7561 cttatcaaca actggctgct gcatatccgc gatatctggt tcaaacatag ctcattgctc
     7621 ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg aactgaacgt catggaacag
     7681 gtgtataacc tgggccactc caccattatg caatcagcgt ggaaacgcgg gcagaaagtt
     7741 accattcacg gctgggccta cggcattcac gacggcttgc tgcgtgatct ggatgttacc
     7801 gccaccaacc gcgaaaccct tgagcaacgt taccgtcacg ggatttccaa cctcaagctg
     7861 aaacacgcca accacaaata aaaatgccat gccggatgca acacatccgg caacttcaca
     7921 cttactcgtc cagcagaatc actttgccaa tatacggcag atgacggtaa cgctgtgcgt
     7981 aatcaatgcc gtaacccacc acaaactcat ccgggatcga gaaaccgata aattcaaccg
     8041 ggacgttcac ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc aacgacttcg
     8101 gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt attccccgag tcgatgatat
     8161 cttcaacaat cagcacgtcc ttgccacgga tatcttcatc cagatctttg aggattttca
     8221 catcacgggt ggtggacatg ccgctaccgt agctggaggc ggtcataaag tcgacttcat
     8281 gagatacctg aacttcacgg cacaggtccg ccataaacat aaatgagcca cgcagcagac
     8341 ccaccagcac catatcgctg ccgctgtctt tgtaacgctc agtaatctga cgacccagtt
     8401 cggcgatacg cgctttaatc tccgcttcgg ggatcattac ttctacagta tgtttcatat
     8461 ctctaaccat atgatttaaa aacaaatcac ttcgcacggg caggttttgg caccgcaaca
     8521 aagcgcaaga cacgcagtat accagtaaaa ctattcattc gcggaatctg ttaataaagc
     8581 tcgaattgtg atgacggtca cacatgttaa aatctataat taattgctac taaaatatta
     8641 atgaattgaa atggtgtctc tttatggcaa ttaacaatac aggctcgcga cgattactcg
     8701 tcacgctaac agcccttttt gcagcgcttt gcgggctgta tctactcatt ggcggaggct
     8761 ggctggtcgc gattggcggc tcctggtact accctatcgc tggccttgtg atgctcggcg
     8821 tcgcctggat gctgtggcgc agtaaacgcg ccgcgctttg gctatacgca gccctgctgc
     8881 tcggcaccat gatttggggc gtctgggaag ttggtttcga cttctgggcg ctgactccgc
     8941 gcagcgatat tctggtcttc ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc
     9001 tggtcattcc tgccagcggc gcagttgccg cactggtggt cgcactgctg attagcggtg
     9061 gtatcctgac ctgggccgga tttaacgatc cgcaggagat caacggcacc ttaagcgccg
     9121 atgccacacc tgccgaagct atctcccccg ttgccgatca ggactggcct gcgtatggcc
     9181 gcaatcagga aggtcaacgc ttttcaccac tgaagcaaat tcacgccgat aacgtccaca
     9241 agctgaaaga agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac gatccgggtg
     9301 aaatcaccaa tgaagtgacg ccgattaaag tgggcgatac cctttacctg tgtaccgctc
     9361 accagcgcct gtttgcgctt gatgccgcca gcggcaaaga gaaatggcat tacgatcctg
     9421 agctgaaaac caacgagtct ttccagcacg taacctgccg tggcgtctct tatcatgaag
     9481 ccaaagcaga aacggcttcg ccggaagtga tggcggattg cccgcgtcgt atcattcttc
     9541 cagtgaatga tggtcgcctg attgcgatta acgctgaaaa cggcaaactg tgcgaaacct
     9601 tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt
     9661 atgagccgac ttctccgcca attatcaccg ataaaaccat cgtgatggcc ggttcagtca
     9721 ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg
     9781 gggagctgct gtgggctttt gatccgggcg cgaaagatcc gaacgcaatc ccgtctgacg
     9841 aacacgcctt tacctttaac tcaccaaact cgtgggcacc agcggcctat gacgcgaagc
     9901 tggatctggt ctatctgccg atgggcgtga ccacgccgga tatctggggc ggtaaccgca
     9961 caccggaaca ggaacgttat gccagctcga ttctggcgct gaatgccact accgggaaac
    10021 tggcgtggag ctaccagacc gttcaccacg acctgtggga catggatctt ccggcacagc
    10081 cgacgctggc ggacatcacc gttaatggtc agaaagtgcc gattatttac gctccggcga
    10141 aaaccggtaa catttttgtg ctcgatcgtc gtaatggcga actggtggtt ccggccccgg
    10201 aaaaaccggt tccccaaggt gctgccaaag gcgattacgt tacccctact caaccattct
    10261 cggaactgag cttccgtccg acgaaagatt tgagcggtgc ggatatgtgg ggagccacca
    10321 tgtttgacca actggtgtgc cgcgtgatgt tccaccagat gcgctatgaa ggcattttca
    10381 cgccgccatc tgaacagggt acgctggtct tcccgggtaa cctggggatg ttcgaatggg
    10441 gcgggatttc cgttgatcca aatcgtgaag tggcaattgc caacccaatg gcactgccgt
    10501 ttgtttcgaa actgctcccg cgtggtcctg gcaacccgat ggagcagccg aaagatgcca
    10561 aaggcacggg tacggaatcc ggcattcagc cacagtacgg tgtaccgtat ggtgtcacgc
    10621 tcaacccgtt cctctcacca tttggtctgc catgtaaaca gccagcatgg ggttatatct
    10681 cggcgctgga tctgaaaact aatgaagtgg tgtggaagaa acgtattggt acgccgcagg
    10741 acagtatgcc gttcccgatg ccggttccgg tgccgttcaa tatgggtatg ccgatgctgg
    10801 gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca gataactacc
    10861 tgcgcgctta caacatgagc aacggtgaaa aactgtggca gggtcgttta ccggcgggtg
    10921 gtcaggcaac accgatgacc tatgaagtga atggcaagca gtacgtggtg atctccgcag
    10981 gcggtcacgg ttcatttggt acgaagatgg gcgactatat tgtggcttat gccctgccgg
    11041 atgatgtgaa ataaccataa tgcccggaga aatccgggca tatttccgaa tacggtcttt
    11101 ttataccgta aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc
    11161 catataagca tgttctttcg gtgcgttgtg attaaacttc accagcactt cactgacatt
    11221 gccttctacc ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt
    11281 ttctgacaag atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc
    11341 cacgccagag ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt
    11401 catatcaaac gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg
    11461 gttcatatga ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc
    11521 ggccatcgcc tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat
    11581 cgggtccata gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg
    11641 caggctactt aatgtgtcag gcaaagcacc ggaggcacta atagcaatcg gctgaatccg
    11701 cattaccgga tgcggcttgt caaacggtgc aatcgccatc cccatctggc tgaccggcag
    11761 cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc
    11821 catcagcacc ggcagctcgc tcaccttcac cggttcaggt agcagaccac cgtcgctggc
    11881 aatcacatac agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc
    11941 attgagcaaa cgcaggcgca gccaaccacg cggggcagcg tgttgtgggt agattgcacc
    12001 gttggtcagc agcgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata
    12061 atcaatctgc ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat
    12121 accccactgt tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc
    12181 agccagcccc atcgccacct gtcgccggtc ttgccatgtt gatgtggatg gaaccagcag
    12241 gtagcggcag gttgatcaac gttcaacgtc accgagcgct tgccacctgg cggaataatt
    12301 ccctgtgggc cgccgtcgac gtcacccggt acttccagcc cgtgccagtg caacgtcgtc
    12361 tcttccgcca gttggttgta gatatcaacc gttaccgctt tgccgcgctg taatttcacc
    12421 gccggcccca gcagattgcc gttatagccc caggtagttg cagttttctc gccaaaggtg
    12481 gactgacctg cgccaatagt taactgaatg cgattacggg catcggtcgt gagcaaatca
    12541 gggatcggta acgttgggcg ttccgccgca aagactgcac ggctccacag tggcaaggct
    12601 gaagccacac ccagtgcgac ggaatatttc aagaaatcac gacgttgcat agttatttcc
    12661 ttattcttaa gcaggcaaaa caaaatcaaa cgttgagcat agtccttccc cttacgggaa
    12721 ggtcaagccg cagacacaat ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc
    12781 taacgtttat cttctttaag cccctggtag aagcaatgaa gacgtttttc agaacagtgt
    12841 tattcggcag cctgatcgcc gtttgcgcaa acagttacgc gctcagcgag tctgaagccg
    12901 aagatatggc cgatttaacg gcagtttttg tctttctgaa gaacgattgt ggttaccaga
    12961 acttacctaa cgggcaaatt cgtcgcgcac tggtcttttt cgcccagcaa aaccagtggg
    13021 atctcagtaa ttacgacacc ttcgacatga aagctctcgg tgaagacagc taccgcgatc
    13081 tcagcggcat tggcattccc gtcgctaaaa aatgcaaagc cctggcccgc gattccttaa
    13141 gcctgcttgc ctacgtcaaa taatccctga tacctttttg tagaaatatt gaccgtgcat
    13201 ccgcggtcaa tgttagctat tatgttgcgc ccttttttta cgggtgttaa caaaggaggt
    13261 atcaacccat ggccgaaaaa aaacagtggc atgaaacgct acacgaccag tttgggcagt
    13321 actttgcggt agataacgtt ctgtatcatg aaaagaccga tcaccaggat ctgatcattt
    13381 ttgagaacgc tgcatttggt cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc
    13441 gcgacgagtt tatctatcat gagatgatga cccatgttcc gctactggcc cacggtcacg
    13501 cgaaacatgt gctgattatc ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac
    13561 ataaaaacgt tgagtcaatc acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc
    13621 gtcagtatct gcccaaccat aacgccggta gctacgacga tccgcgcttt caactagtga
    13681 tcgacgatgg cgtcaatttc gttaatcaaa ccagccagac ctttgatgtc attatctccg
    13741 actgcaccga tcctatcggt cccggcgaaa gcctgttcac ctcagcattt tatgaaggct
    13801 gcaaacgttg cctgaatcct ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac
    13861 agcaggaaga agccatcgac agccatcgca aactcagcca ttacttcagc gacgttggct
    13921 tttatcaggc ggcgatcccg acctattacg gcggtatcat gacttttgca tgggcgacag
    13981 ataacgacgc cttacgccat ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg
    14041 gcctgaaatg ccgttattac aatccggcaa tccatacggc agcttttgcc ttacctcagt
    14101 atctgcaaga cgcactggct tcacagccgt cctaaggaga agataagaaa ttgaaaaaac
    14161 tgaaactgca tggctttaat aatctgacca aaagtctgag tttttgtatt tacgatatct
    14221 gctacgccaa aactgccgaa gagcgcgacg gttatattgc ttatatcgat gaactctata
    14281 atgccaaccg tctgaccgaa atcctgtcag aaacctgttc cattatcggg gccaatattc
    14341 ttaacatcgc ccgccaggat tacgaaccac agggtgccag cgtcactatt ctggtgagtg
    14401 aagaaccggt tgacccgaaa ctcatcgaca aaacagaaca ccccggccca ctgccagaaa
    14461 cggtggttgc tcatctcgat aaaagtcata tttgcgtaca tacctacccg gaaagtcatc
    14521 ctgaaggcgg tttatgtacc ttccgcgccg atattgaagt ctctacctgc ggcgtgattt
    14581 ctccgctgaa ggcgctgaat tacctgatcc accagcttga gtccgatatc gtaaccattg
    14641 attatcgcgt gcgcggtttt actcgcgaca ttaacggtat gaagcacttt atcgaccatg
    14701 agattaattc gattcagaac tttatgtctg acgatatgaa ggcgctgtat gacatggtgg
    14761 atgtaaacgt ctatcaggaa aatatcttcc ataccaagat gttgcttaaa gagttcgacc
    14821 ttaagcacta catgttccac accaaaccgg aagatttaac cgacagcgag cgccaggaaa
    14881 ttaccgctgc gctgtggaaa gaaatgcgcg agatttatta cgggcgcaat atgccagctg
    14941 tttaacggct ctggcggagc tcccaggctc cgccagattt atttacttct gctgcacgaa
    15001 attgcggtaa gccgccacga cctgcagaaa atcctcaacg ccgcatagcg acaggctttc
    15061 ttcgtcgtag tagttcatcc cctcttccat ttcatcgcca gcgaattcca gttgattggc
    15121 gcgaaccatc acctcttcag cgtccatcca cagggtgtat tcatgccctg cccgttgcca
    15181 ggaccgttcg ctacctttca gtgcgtgcgc tgcttgttcc acttcatcaa gcaaggccag
    15241 gttttctttc acctcttcat taaaccagtg cccgaccact tcatgcccca tggacatacg
    15301 cacctttacc actccggtaa tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc
    15361 tcccggcaat gcgccgaaat cgcatcgcgc cattgatgta attatcgcag ccttaccgga
    15421 gaaaaacagc ggaacggcga tggtttagaa ataaaaaatg cccgggaatc tcattcaccg
    15481 ggcattggtg tcgtttatgc gcagcgcgtg cgcttactta ttacaccgca gtctggaaaa
    15541 tcaccccatc ggctttttcg gtgtactgag aaagctggtt gaagttcaga taacgataag
    15601 tatcaacggc agttttatct acttgcgcaa cgtaggtctg gtactcttcc ggcgtcggca
    15661 gtttgccaat cagcgccgca acagccgcca gttctgcaga agccaggaag acattcgcgc
    15721 cagtacccaa acggttcggg aagttacggg tagaggtgga aaccaccgtc gcaccgtctg
    15781 ctacacgcgc ctggttaccc atacacaggg aacagcccgg gatctcgata cgagccccgc
    15841 tcttaccgaa gacgctgtag tagccttctt ccgtcagttg cgcggcgtcc atacgggttg
    15901 gcggtgccac ccacaggcgg gtcggcaact gacctttgtg cgcatccagc agtttaccag
    15961 cggcacggaa gtgaccgatg ttagtcatgc aagaaccgat aaatacttcg tcgatcttct
    16021 cgccctgtac cgcagacagc ggacgtgcgt catccggatc gttcggagca cacaggattg
    16081 gctctttaat ctccgccaga tcgatgtcga tcactgccgc gtattccgca tctgcatcgg
    16141 cttccagcag ctcaggattc gccagccatt tttccatgcc ctgaatacgg cgttccaagg
    16201 tacgacgatc gccgtaacct tccgcgatca tccacttcag caggacgatg ttggagctca
    16261 ggtattcgat gatcggttct ttgttcagct tgatagtaca accagcggca gaacgctcgg
    16321 cggacgcatc ggttagctca aaggcctgct caactttcag atccggcaga ccttcaattt
    16381 ccagaatgcg accagagaag atgtttttct tgcctttctt ctcaacggtc agcagacctt
    16441 gtttgatcgc atacagcgga atggcgtgta ccagatcgcg cagggtgatg cccggctgca
    16501 ttttgccttt gaagcgcacc agaacggatt ccggcatatc cagcggcatt acgccagttg
    16561 cggcagcaaa cgccaccaga ccagaacccg ccgggaaaga gataccgatc gggaaacggg
    16621 tatgggagtc accaccggta ccgacggtat ccggcagcag catacggttc agccaggagt
    16681 gaatgacgcc gtcacccgga cgcagcgaca caccgccacg gttcataatg aagtccggca
    16741 gcgtgtggtg cgtgttcacg tcaactggct tcggatacgc cgcggtgtgg cagaaagact
    16801 gcatcaccag gtcagccgag aagcccaggc acgccaggtc tttcagttca tcacgggtca
    16861 tcgggccggt ggtgtcctga gagcctacag aagtcatttt cggttcgcag tacgcgcccg
    16921 gacgaatgcc tttcacgcca caggcacggc ctaccatttt ctgcgccagc gagaagccgc
    16981 gatcgctctc agcgacatct ttcgcctgac ggaacacatc actgtgcggc agaccaagtg
    17041 cttcacgagc tttggtggtc aggccacgcc cgataatcag cggaatacgg ccgccagcac
    17101 gcacttcatc aatcagcacg tcggttttca gttcgaaggt cgccagcagt tcgcctgttt
    17161 cgtggttacg cacttcacct ttgtacgggt aaacgtcaat cacgtcgccc atgttcaggt
    17221 tagagacgtt gacttcgatt ggcagtgcac ccgcgtcttc catcgtgtta aagaagatgg
    17281 gtgcaatttt accgccgagg cacaaaccac cgccgcgttt gttcggcaca tgtggaatat
    17341 catcgcccat aaaccacaga acggagttcg tggcggattt acgcgatgaa cccgtaccca
    17401 caacgtcacc gacgtacgcc agcgggaaac ctttctgttg cagagcttca atctgtttga
    17461 tcgggccaac aacacctggc tggtctggct caataccttc acgggcgttt ttcagcatcg
    17521 ccagcgcgtg cagtgggata tccgggcgtg accacgcatc cggtgccgga gaaaggtcat
    17581 cggtgttggt ttcgccagtg actttaaaga cggtaacggt cagtttttca gccagcgccg
    17641 ggcgattcag gaaccattcg gcatccgccc aggactgcat tacctgcttc gcatattcgt
    17701 tgcctgcttt cgctttctct tctacgtcat agaagttatc gaacatcagc agcgtgtgag
    17761 aaagtgcttt ggcagcaata ggtgccagtt tggcatcatc cagcgcgtcg atcagcggat
    17821 gaatgttgta accaccctgc atggtgccca gcagttcgat agctttttcc ggagtcagca
    17881 gaggggattt agcttcgcct ttagcgatag cagccaggaa gcctgctttt acataggcgg
    17941 cttcatcgac gcctggggga acacggttgg ttaacagatc taacaggaat tcttcttcgc
    18001 ccgcgggcgg gtttttcagc agctctacaa gcgcggccat ttggtttgca tccaggggtt
    18061 tgggcgcaat cccctcagcg gcacgctcag ctacgtgctt acggtattct tctagcacga
    18121 cggttctcct cgctctcatt gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag
    18181 acagcagttt gtagggtaaa tgcccagtac cgcaacgggc agaatagcag gattttgacg
    18241 acgtgttaat ctgtttacaa aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt
    18301 ctagagattg tttccctccg taaaaacttc gttaattcgc atggtgataa tcacctttct
    18361 caacgaatcc caagattagt caaaatttaa actaccgcct ctttatactc ggattcacag
    18421 cacctgcggg tggcagttcg ccgaccattg cgatttcctt gagatccgaa ttatgaaaat
    18481 gactttgccg tttaaacccc atgtgctggc gctaatttgc agtgccgggc tttgtgctgc
    18541 ctctgccggg ctatatataa aaagccgcac agtggaagcg cctgtagaac cgcaatcgac
    18601 acaactggct gtgtctgacg ctgccgctgt tacgcttcct gcaacggttt ccgcacctcc
    18661 cgtaataccc gcagtcgtca aatccgcatt cagcactgca caaatagatc aatgggtcgc
    18721 acccgtcgcg ctgtatcccg acgccctact ttcgcaggtg ctgatggcat caacctatcc
    18781 gacaaacgtt gctcaagcag tgcaatggtc gcacgataat ccacttaaac aaggcgatgc
    18841 tgctattcag gcggtatctg accagccgtg ggacgccagc gttaaatcac tggtggcctt
    18901 tccacaattg atggcattga tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc
    18961 ttttctggcg cagccgcagg acgtgatgga ctcggtacag cgattgcggc aactggcgca
    19021 acaaaccggc tcgctgaagt catcaaccga acagaaagtt attaccacaa cgaagaaagc
    19081 tgtaccggta aaacagacag tcacggcacc cgtcatacca tccaataccg ttttaactgc
    19141 caaccccgtc attacagagc cggcaacaac cgtcatttcc attgagcccg ccaatcctga
    19201 tgtggtctat attcccaact acaacccaac cgtggtttac gggaactggg ccaatactgc
    19261 gtatccgccg gtttatctgc caccaccagc cggagaaccg tttgttgaca gctttgtgcg
    19321 cggattcggc tatagcatgg gcgttgctac cacgtacgca ctattcagca gcatcgactg
    19381 ggatgacgac gatcatgacc atcatcatca tgacgatgat gattatcatc accacgatgg
    19441 cggtcatcgt gacagtaatg gctggcaaca caacggcgac aacatcaata tcgacgtcaa
    19501 caatttcaac agtatcaccg gtgagcatct tactgataag aatatggcat ggcggcacaa
    19561 tccaaactac cgtaatggtg tgccctatca tgatcaggat atggcaaagc ggtttcatca
    19621 aaccgatgtc aacggcggaa tgagtgccac gcagctacct gctccaacac gcgacagcca
    19681 gcgtcaggcg acagcaagtc agtttcagca acgaacacac gccgcccccg tcattacacg
    19741 agatacccaa cgtcaggcag cggcacagcg gtttaatgaa gctgaacact atgggagcta
    19801 tgacgacttc cgcgacttca gccgtcgcca accactgacc cagcagcaaa aggacgccgc
    19861 tcgtcagcgt tatcagtcgg cctctcctga gcagcgccag gcagtccgcg agaaaatgca
    19921 gactaacccg cagatccagc agcgaaggga ggcagcgcgc cagcgtattc agtccgcctc
    19981 gcctgagcag cgccaggcgg ttcgcgagaa aatgcagact aacccgcaga accagcagcg
    20041 aagagaggca gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtgtttaa
    20101 ggaaaaagta cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat
    20161 acaatcggca tcacctgaac aacgtcaggt ttttcgggag aaagttcagg agagccgccc
    20221 acaacgtcta aacgacagta accatactgt caggctgaat aacgagcaac ggtcaacagt
    20281 acgcgaacgt ctctctgaac gtggagcaag aagactggaa aggtaaattg cagacgtaaa
    20341 aaaagcggcg tggttagccg cttttttaat tgccggatgt tccggcaaac gaaaaattac
    20401 ttcttcttcg ctttcgggtt cgggttcggc aggtcggtaa tgctaccttc gaacacttct
    20461 gccgccaggc ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct
    20521 tcagcatcac aacccatttc gattgccagc ccgatttcac ccagcagctc gccgccgttg
    20581 gtaccaacaa tcgcaccacc gatcacgcgg tgagattctt tgtcgaaaat cagcttggtc
    20641 ataccgtctg cgcagtcaga agcgatagca cgaccagaag cagcccacgg gaaggtggcg
    20701 gtttcatagc tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact
    20761 tctggttcgg tataggcgat ggacgggata actttcggat cgaagtagtg tttcttaccg
    20821 gcgataactt cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga
    20881 ccgacgatat cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg
    20941 cggatgaaac cacggtcgtc aacttccacg ccagctttgc ctgcgtcgag gtttttaccg
    21001 ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agctggtgct
    21061 tttttgcctt ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg
    21121 gtttccagca tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct
    21181 ttgtcagctg ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc
    21241 gcgtggtaaa cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt
    21301 tctggcactt ctttcagttc cagcgcgtca gtggagtccc agatacgcgg atcttcatgc
    21361 ggaataaacg gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg
    21421 atcacggttt tgccgttctc accttcaact tccagggtgt tagccccggt gaatttaccc
    21481 agaccgttga ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc
    21541 tgattgatca ctttctcttt ccaggtacga atcttgtcaa tatcggtttt cggttcgccg
    21601 aagacgatac cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc
    21661 agtgctttag aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt
    21721 tctacgatta cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg
    21781 gggcctgccc caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata
    21841 tatttatctc cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc
    21901 cctcacccca gtcacttact tatgtaagct cctggggatt ctggcgcttg ctaccttgct
    21961 gcacccagaa atccataggg tatgtgaagt aataccctaa ccaccaccgg gtcgttctat
    22021 ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc
    22081 tattcgctaa taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc
    22141 ggctctttta cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta
    22201 atgaaacggg caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc
    22261 agcatcagac gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa
    22321 acgccgagga tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg
    22381 ccgccgatgc tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg
    22441 tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg
    22501 ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg
    22561 ttgatgtatt tcttcagggt cagacgctga ccgtcttccg acagcgaact attgaagcga
    22621 ggcatctgct caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc
    22681 acatccagct tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg
    22741 gtgatatcgg ttttgtcgaa gtgggtaaca tgcgggatca tcacccagtt acggctcagg
    22801 ttagcaccag aaattttctg gatacggccc agttccactt cttcgatttc gccaaacttg
    22861 ctgaagtcca ccttcggcca cggcagcatg ccagggatac caccgccagt cgccgccgga
    22921 gctgcttctg cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata
    22981 cgacctttac ggccagtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga
    23041 cggcggatca gcggggtcgc gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct
    23101 tccgctttcg cagccggagc tgctgccgga gcttcagctt ttgctgccgg agccggcgct
    23161 gccgcttcct gtttcgcagg agctgccgca ggtgctgcgc cttcaacttc gaagatcata
    23221 atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct
    23281 gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtaat cagtgactgt
    23341 tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg
    23401 ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcgggggt cgctgccgga
    23461 gtcgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc
    23521 ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggta
    23581 cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt gatcagcgac
    23641 tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc aacttcgtcg
    23701 ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc tgctaccgga
    23761 gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa
    23821 tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct
    23881 ttaacgatac ccgcctgcgg agatggaact tccatagagg ctttgtcgcc ttctacggtg
    23941 atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca
    24001 acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt
    24061 acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa
    24121 ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat
    24181 aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc
    24241 catcagtacc cagtacgcga tagtcgtcag ccggtacgta agtacggacc tgctcagcga
    24301 acagtttcat atagtcggta gatgccactg ccggcgcgtc gttcatcacc tgagcgatat
    24361 acggaacgcg cggagtttcc agcggatgca gcatgttcca gcgttcacaa tcctgaccat
    24421 cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg ccgtaatctt
    24481 tcgccaggat ctctgccgct tcacggacgt gacgcaggat ggaaccggag cccagcagct
    24541 gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt
    24601 cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta gtgatgtagt
    24661 agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc ataatgacag
    24721 caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa
    24781 tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag
    24841 taccgccgat caggaagcca cgcgcttgct ggtctccagc cgcccagcac agatcgccga
    24901 tacgctggaa gccgaacatc gagtaataga tgtagaatgg gatcatcggc agattgttcg
    24961 tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg ttgatccctt
    25021 cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg
    25081 gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac
    25141 cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct
    25201 tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagagctct ttgctctgct
    25261 cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg
    25321 gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga taggtatgct
    25381 cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg
    25441 gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc ttaacctggt
    25501 gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa
    25561 ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct
    25621 tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca
    25681 gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca ccatctttcg
    25741 atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc
    25801 tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg atcacgttcc
    25861 agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg
    25921 ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc agtttttcac
    25981 gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga
    26041 acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga
    26101 atttagcctg gtaaatagca ccaatcggac ccagacccat agatacggtc gggaactgcc
    26161 agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct
    26221 gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca cgggcgtaaa
    26281 cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg
    26341 cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca
    26401 tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg atggcgttcc
    26461 agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt
    26521 cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct acgtttacac
    26581 cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt
    26641 cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt
    26701 catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct
    26761 ggaacctgtc ccattgaact ctcgccagaa agctcaataa gacaggttct acgtttagtt
    26821 accgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat
    26881 cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct ctcttcacgg
    26941 cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct
    27001 tcttccggct taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc
    27061 ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgggccaac
    27121 atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg
    27181 gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc
    27241 agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc
    27301 gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt
    27361 gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg
    27421 ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt
    27481 tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag ttcgcgttcc
    27541 ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc
    27601 tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt
    27661 aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca
    27721 aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta
    27781 acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtag actttacgta
    27841 ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc gattcactta
    27901 ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcactctgc atattgagag
    27961 gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc aaccatgcat
    28021 aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag tctttttgta
    28081 acaattcaaa cttccttgat gtaaataaat caatacaaca aacggaattg caaacttaca
    28141 cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg atggaaggtc
    28201 aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc
    28261 tgggtggcgc gatagggaca gggctattcc tgggtagcgc ctccgtaata cagtccgcag
    28321 ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg atcatgcgtc
    28381 agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac tttgcttata
    28441 aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta ctgtacgttt
    28501 tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg tatccggaaa
    28561 tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc aacctgacca
    28621 acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt attgcggtag
    28681 tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc ccgcaggcga
    28741 gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc ggactggtga
    28801 tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg atcaccgcag
    28861 cagaagctga taacccggag caaagtatcc cgaaagcaac taaccaggtt atctaccgca
    28921 tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg tggacccgcg
    28981 ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat acctttgtgg
    29041 cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac agctgcgtat
    29101 attgcaacag ccgtatgctg tttggtctgg cacaacaggg gaacgcgcca aaagcgctgg
    29161 cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg ctggttacag
    29221 cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg ttaatggcac
    29281 tggtggtatc cgcactggtg atcaactggg cgatgatcag tctggcgcat atgaagttcc
    29341 gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg
    29401 gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg atgaccccag
    29461 gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt atcggctatc
    29521 tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac gccctcaccc
    29581 gtacagggtg agggcaataa tctctacacc aacgccccgt aaatcgtcag tagcgcaatc
    29641 accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc
    29701 accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt taatacctga
    29761 tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc tttctcacca
    29821 tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc cagtacatga
    29881 agcactgcat caatgccaga ctgtaaacga tgatgcggcg tctggtatcg cgccagccag
    29941 tattgccatg cacgcaagaa cgcatacccc atcagcgtaa cgggtcccca ggttcccccc
    30001 acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag caatgcattt
    30061 tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg
    30121 agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc tgtcagataa
    30181 gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat cagcagccac
    30241 accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag taaaaaagtc
    30301 acacccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg tttcacccgc
    30361 cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt aaacaggcgc
    30421 tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg ctgaccagcg
    30481 cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc ggcgcaatat
    30541 cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt gcccgcgtaa
    30601 ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg ccttcaagct
    30661 caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag actcccgcat
    30721 gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg cgaatcaaac
    30781 agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa
    30841 tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa
    30901 tattgtgcac caccagcagg gtgggtgttt cgtcatccag gcggcaatcg taatgtggtg
    30961 agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag gatctcctta
    31021 tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt cgttagctat
    31081 ctggagtttt aacatgccgc ctcgccgcta caaccctgac acccgacgtg acgagctgct
    31141 ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg aagatttagg
    31201 cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa attctcgctc
    31261 tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt ggcaaacgct gggttgaaga
    31321 ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg atgacggcga
    31381 tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg tgctgttaac
    31441 gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca gtaaggtacg
    31501 ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc gcaaaacctt
    31561 acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg
    31621 tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgcct ccggctcagt
    31681 gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat gcgccagtag aagtcgaagt
    31741 agagaatctg gaagaacttg atgaggccct gaaagccgga gccgatatca tcatgctgga
    31801 taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacgaca aggcgctact
    31861 ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa cgggcgtgga
    31921 ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt caatgcgttt
    31981 tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc ccttccccaa
    32041 ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata aaaaacctcg
    32101 gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt ttgccaaagt
    32161 agcaccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt acacttatcg
    32221 aactgatggt ggttattggc atcattgcca ttttaagcgc cattggtatt cccgcttatc
    32281 aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg ccttaccgta
    32341 ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac ggtggcagca
    32401 atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg gcaaagggcg
    32461 tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg acgccaggtt
    32521 gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa agtgacagcg
    32581 cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa ggagcggcaa
    32641 tgaatattcc acagctcacg gccctgtgcc tgcgttatca gggagtcttg ctggatgcca
    32701 gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc acatgagttg ctggacgcat
    32761 tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa caaatggaag
    32821 gtcacgccag tcgcacacaa cagacattgc ccgtagctgt tcaggagaag catcagccca
    32881 aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgcgcg tctgatattc
    32941 atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc gtattgcatc
    33001 ctttaccgga tgtttcaccg gatgccggag tcgcattaac agccagatta aaagtgctgg
    33061 gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact gtcgaactgg
    33121 caggaaacac cgtctcattt cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg
    33181 tattaaggtt gttacagcag gtgagccagg cactggatgt taacacgctg ggaatgcagc
    33241 cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg gtgctggtaa
    33301 ctggccctac aggcagcggc aaaacggtca cgctttatag tgccctgcaa acgctgaata
    33361 ccgctgacat taatatttgt agcgtcgaag atccggttga gatccccata gccggactaa
    33421 accagacgca aatccatccg cgtgccgggc tcacctttca gggtgttttg cgtgcgttat
    33481 tgcgccagga tcctgacgtc atcatgatcg gagagatccg cgatggcgaa acggcagaaa
    33541 ttgccattaa agccgtgcaa accggtcacc tggtgttgtc cacgctacac accaattcca
    33601 ccactgaaac gctggtacgt ttacagcaaa tgggggttgc ccgctggatg ctatcatcgg
    33661 cgcttacgct ggtaatagcc cagcgtctgg tacgtaaact ttgcccacat tgtcgccgac
    33721 agcaagggga gcccatccat attccagaca ctgtatggcc gtcgccgctg ccccactggc
    33781 aggcacccgg ttgtgtacat tgctaccacg gtttttatgg tcgtaccgcc ttgtttgaag
    33841 tcctgcccat tacaccagcc atacgccagc ttatttccgc taatactgaa gttgaatcgc
    33901 tggaaacgca cgcccgacag gcgggtatgc gtacgctttt tgaaaacggc tgcctggccg
    33961 tggagcaagg cttaaccacc tttgaagagt taatccgcgt actggggatg ccgcatggca
    34021 agtaagcaac tctggcgctg gcatggcata accggcgacg gcaatgcgca agatgggatg
    34081 ctatgggcag agagccgtac tttactgctt atggcactac agcaacagat ggttacccca
    34141 ctaagcctga agcgaatcgc catcaattct gcgcagtggc gaggagataa aagcgcggaa
    34201 gtcattcatc aactggcgac gctacttaaa gccgggttaa cgctttctga agggctggct
    34261 ctgctggcgg aacagcatcc cagtaaacaa tggcaagcgt tgctgcaatc gctggcgcac
    34321 gatctcgaac agggcattgc tttttccaat gccttattac cctggtcaga ggcatttccg
    34381 ccgctctatc aggcgatgat ccgcacgggt gaactgaccg gtaagctgga tgaatgctgc
    34441 tttgaactgg cgcgtcagca aaaatcccag cgtcagttga ccgacaaagt gaaatcagcg
    34501 ttgcgttatc ccatcatcat tttagcgatg gcaatcatgg tggttgtggc aatgctgcat
    34561 tttgttctgc cggagtttgc cgctatctat aagaccttca acaccccact accggcacta
    34621 acgcagggga tcatgacgct ggcagacttt agtggcgaat ggggctggct gctggtgttg
    34681 ttcggctttc tgctggcgat agccaataag ttgctgatgc gccgcccgac ctggcttatc
    34741 gcgcggcaga aattgctgtt acgcatcccg attatgggtt cactgatgag gggacaaaaa
    34801 ctcacgcaga tctttacgat tctggcgctg acacaaagtg caggcattac ttttttacag
    34861 ggcgtagaga gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact tctgacacaa
    34921 atccagcacg atatcagtaa cggtcatccc atctggctgg cgctaaaaaa tgccggtgag
    34981 tttagcccgc tctgtttgca attagtgaga acaggagagg catccggctc gctggacctc
    35041 atgttagaca acctcgccca tcatcatcgg gataacacaa tggcgctggc ggataacctc
    35101 gcagccttac tggaaccggc gttgctgatc ataacgggag gaattatcgg tacgctggtg
    35161 gtggcaatgt atctgccaat tttccattta ggcgatgcga tgagtgggat gggataacgc
    35221 tggcgtgttg ctccacgcca gcgttgggag attacaggtt gttgaagatg cggttttctt
    35281 gttccagcac acgaataaac gtggtgcgct tggtcagctc tttcaggcgt gaagccccaa
    35341 cgtatgtaca agctgaacgc aggccgccca aaatatcacg cgcggtattt tcaaccgggc
    35401 ctcgcagcgg cagcttgacg gttttacctt ctgctgcgcg gtattccgca acgccgccaa
    35461 cgtgacgttt catcgcagac tcggagctca tgccgtagaa cagcataaat ttctcgccgt
    35521 tctcctcaac gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg ccaagcatga
    35581 cgaaatcggc accgccgcca aaggctttcg ccacatcgcc cggcgtggta cagccaccgt
    35641 cgctgacgat cattccgccc agaccgtgcg ccgcatcggc acattcgatt accgcagaaa
    35701 gctgcggata accgacgcct gttttgacgc gagttgtaca aacagaacct gggccaatgc
    35761 caactttaac gatatcagca ccggagagga taagctcctc acacatttca ccagtcacta
    35821 cgttgccagc acaaatggtt ttggtcggcc acgcttcacg cgctttcgca acgaactgca
    35881 cgaagtgttc ggaataacca ttcgccacat caatacaaac gaagtttaat gccgggttca
    35941 ggtcgagaat ctgtttagtt ttttcgaaat ccgcatcaga cgtgccggta gaaaccatca
    36001 catgtttcag cacatcagcg gaagaattgt tgataaacgc ttgccactct tcgacagaaa
    36061 agtgtttatg cacagcagtc agaatatcaa aagaagccag cgcagaggcc atagaaaatg
    36121 tgcctacggt gtccatattt gcggcgataa tcggcacgcc ggaccagctc tgacctgaat
    36181 gtttgaaggt gaattgacgt tccagttcaa catcggaacg gcttttaaga gtggagcgtt
    36241 tagggcggat gagaacgtct ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg
    36301 gattcctggg gttaatggct ataagggtaa gagcgcaact ccagtgacgc tatcatacgc
    36361 actaatcaat gcggcgcaag actgcgaaat tgtctttttt tacgctacaa tcccataaat
    36421 ttacctggta aatagtgagc gaacgtttca tcaatttcac tttcgcgcgc tcatttttaa
    36481 cgttttgata atcatgatga attcccggga atataaatta tggggtatat agttgcctta
    36541 acgggaggca ttggcagtgg taagagtacc gttgccaatg cgtttgctga tctcggaatt
    36601 aacgtcattg atgccgatat tattgcgcgt caggtggttg aaccaggtgc acctgcgcta
    36661 catgccattg ctgatcactt tggcgaaaac atgattgctg ctgatggaac attgcagcgc
    36721 cgggccttgc gcgagcggat cttcgccaac ccggaagaga aaaactggct taacgacctg
    36781 ctgcatccgc tgattcagca agagacgcaa caccagatcc agcaagcaac ttccccctat
    36841 gtactgtggg ttgtgccatt gctgatagaa aactcactgt ataaaaaagc gaatcgagtg
    36901 ctggtggtgg atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta
    36961 actcgcgaac atgtcgaaca aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc
    37021 gtggcagatg acgtcattga taataacggc gcaccggatg ctatcgcatc ggatgttgcc
    37081 cgcctgcacg cacactattt gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa
    37141 tgcagaccca ggtccttttt gaacatccac taaatgaaaa aatgcgtaca tggctgcgca
    37201 ttgagttttt gattcagcaa ctcaccgtta atttacccat cgttgaccac gctggcgcgc
    37261 tgcatttctt ccgtaatgtc agtgaattac tggatgtttt cgagcgcggc gaagtccgca
    37321 ctgagctgtt gaaagaactt aaccggcagc aacgtaaact ccagacctgg attggcgtgc
    37381 ctggcgtgga ccagagccgt attgaagcat taattcagca gttaaaagcg gcggggagcg
    37441 tattaatttc cgcgccgcgt atcgggcaat ttctgcgtga agatcgtttg attgctctgg
    37501 tgcgtcagcg actgagcatc ccaggcggct gttgcagctt tgatttacct acattgcaca
    37561 tttggctgca tctaccccag gcgcagcgcg acagccaggt agaaacctgg attgccagcc
    37621 tgaacccgct cacccaggca cttaccatgg tgctggattt aattcgccag tcggccccct
    37681 tccgtaaaca aaccagcctg aatggttttt atcaggataa cggtggcgat gccgacttgc
    37741 tgcgcctgaa tctgtcgctc gattcacagc tttatccgca aatttccgga cataagagcc
    37801 gttttgccat tcgttttatg ccgctggaca ctgaaaacgg acaggtaccg gaacgtctgg
    37861 atttcgaact ggcctgttgc taaggagtaa aaatgtcaga aactattacg gtgaattgcc
    37921 caacctgcgg gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca ttttgctcca
    37981 aacgttgtca gctgatcgac ctcggagaat gggctgctga agaaaaacga atccccagca
    38041 gtggcgatct ttccgaaagc gatgactgga gcgaagagcc aaagcagtga catttgtctg
    38101 atgcctcacg taggcctgat aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg
    38161 tcggatgcgg cgtgaacgcc ttatccgacc tacagacgct taagcttcgc aattaccggt
    38221 tcattggctg gcggaaaatc atcggcatta agaccgacca gcgacatcca ctcaccgggt
    38281 tgcccttctt taccccacgg caccccttcc caactttcga ccagccagaa ccacagagtt
    38341 atatgcctgt ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgtggggta
    38401 atcccgacct cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc gcccatttca
    38461 attttaccgc cgggaaactc cagtttattc gccatgtgcg catctgctgc gcgacgtgtt
    38521 ataaagattt cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt
    38581 gtcttaaacc tataaaaaag gcgcagaatc ctgcgccttt tacttcaact gttagctttt
    38641 attgcaggcg accatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt
    38701 tacgtcctac tttgcgttca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt
    38761 catcctgatg gctaagctgc tgcatttgtg ctaaacgctc ggcttccata cgacgctgtt
    38821 gttccagctc ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa
    38881 cttcatattt caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt
    38941 cctgcttcgg atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca
    39001 tcgctgccag gtgctctttc cacagggagt caagcgtttg cagcatgacg cctttctcga
    39061 agtgacgcat catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact
    39121 gcgccagaat gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact
    39181 cggcaattgg taaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat
    39241 cccacatttc ttccagcgac tgtggcggaa tgtaggcatc aatggtggct ttgaacacat
    39301 cttcacgaat gctgttgatg gtttcgctca catcgctgac atccagcagt tcgttacgct
    39361 gggagtaaat ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac
    39421 gaatgtcgaa gttacgactt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc
    39481 acgggtgttc aatggcttcg cctagcttca tacccagttt acgcatcatg ccggatactc
    39541 ggtcggaagc aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac
    39601 cagcatcccc ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt
    39661 gacgctcggt accgatgata tgcaggccac ctgcttccag taccgcatcg tgacgtacct
    39721 gccagtcggc tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt
    39781 ctgcctgcca gctacctccg agcacaatat ctgtaccacg acccgccatg ttggtcgcga
    39841 tagtcaccgc agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt
    39901 tggcgttcag gacattgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg
    39961 atttttcgat ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga
    40021 tatcttcaat gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat
    40081 ctttacgaat cattggacgg ttggtcggaa caacgacggt atccagtttg tagatggagc
    40141 taaattcgaa agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac
    40201 ggaagtagtt ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac
    40261 cttctttcgc ttccacagcc tggtgcagac catcggacca gcgtcggccc tgcatggtac
    40321 gaccggtgtg ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac
    40381 gggtaaacag cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg
    40441 ccggagagta cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa
    40501 tcagcaccag accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt
    40561 ggccttcgcc ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa
    40621 ttttattcac gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg
    40681 gtgtacgcgc ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt
    40741 tacgctgtac acgttcttca gggctgaacg ccatgttgtc gcgcaggtag tcaaagccgt
    40801 attcgttgtt cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg
    40861 gcatgcccgg caggttgata ccaacagtca ggccaaggaa ttcaaacagc ggacggttgt
    40921 tttcggcgtc acgttgcgcc aggtagtcgt tgacggtaac tacgtgcacg cctttaccgg
    40981 ttagtgcgtt caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca
    41041 tttcggcgat gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac
    41101 gcataccaaa gacgcgctta cttgcctcac gcaccacggc gaaagcttcc gggatcagat
    41161 tttccagcac ttcgcctttt tccagacgcg cacgaaactc tgcggttttc cctttcagtt
    41221 cttcatcgga gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc
    41281 gcatccggcg cagggtgcga tcgttacgac taccgaaaac tttagttaac aatttgatta
    41341 gcataataaa atctcaaacg ccccgcgtta cggagttaat aaaatgaagt aaaggtttat
    41401 tgttgttagg tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg
    41461 ggcgtgctga attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca
    41521 gacggcgtgc cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc
    41581 gcctgaagag gcaaagattc ttcagcaacg ggcagtgttt gcggtgccat tgcgaaagaa
    41641 agatgacgga ttaccgtgcg aatagcatgt tgatgccagt aatcaacgga atagttcgaa
    41701 ttcgggcggc gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa
    41761 ggctcgtggt tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc
    41821 gcaggcaaac ctaaactcgc cgcgaccatc cctaataaga gatgcggcca gaagtagcgt
    41881 ttaccaaact gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgaat
    41941 tcaaacgtat ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa
    42001 gataagttgt gcgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagctctt
    42061 ataaaattca aaaaaaaacg actggttcac ctggccggag agagtgccag atttgccagt
    42121 cgaattttat acgacagtat aaatgtcgtt atgccagtac agctgaaggc gctttgaagg
    42181 ccaacggcag ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc tgtttcgcta
    42241 ggacagcctg cagcagtttg ttattcagtg catgaccgga tttataagcg gtaaatgcac
    42301 caataatatt gtgaccacac atgaacaagt caccgatcgc atcgagcatt ttgtgacgca
    42361 caaattcgtc ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg tcaacaacga
    42421 tggcacaatc gaagctgccg cccaggcaca aaccacggga ctgcagatat tcgatatcac
    42481 gcatgaaacc gaacgtacgc gcacggctga tctggcgcat aaacgcatca gcggagaagt
    42541 tcatcgcata gcgctggttg ctggaatcaa tagccggatg gttaaaatcg atggtgaaat
    42601 ccagcgaaaa accattgtac ggcttaaatt cagcccactt atcgccatct tcgacacgaa
    42661 cagtctcttt gatgcgaaca aattttttgg cgcagttcaa ctcgtcgata ccggcgtcaa
    42721 gcagcaggta tacaaacgga gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa
    42781 cttcgataac aatgttatcg atgcccaggc ccgcgagagc agcattgagg tgctctacgg
    42841 ttgaaatccg tacatcatgc tcgttgacca gacacgtaca gagcatggta tcacgcacag
    42901 atttggcatc ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga tagatgaccc
    42961 cggtgttggc cggcgcaggg cgtaacgtca gggtgacttt cttgccggta tgtaaaccga
    43021 cacccgtcgc ctgaacgata cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc
    43081 aaattaccta tccaaccgaa gtgtactata cattcggcgg gccagtttag cacaaagagc
    43141 ctcgaaaccc aaattccagt caattcttaa tcagcttgct tacgtaggaa tgctgggata
    43201 tccagataat ccggctcttt cgcagtttgc ggcgcattgt cattcactac tttagcaacc
    43261 ggcttctgct cctgggtcag cggagccatc ccatgctgct ggtagcgatc catcactggc
    43321 tgctgaacct gcttattggt caccagagtg atttcaggac gtttgtccat gccgatacct
    43381 gtcgcaacaa cggttacgcg cagctcgtca ttcatatccg ggtcaagaga agtaccgata
    43441 accacagtcg cgttgtcgga agcaaatgca cggatggtgt tacctaccgt ttcgaactca
    43501 tccagacgca ggtcgaagcc cgccgtgatg ttaaccagca cgccgcgcgc gccagacagg
    43561 tcgatatctt ccagcagcgg agaagagata gccatttcag cagcttcttc cgcacggtct
    43621 tcaccgctcg ccacgccaga acccatcatt gcgtagccca tttcagacat tacggtgcgt
    43681 acgtctgcaa agtccacgtt catcaaaccc ggacgagtaa tcagttcggc gataccttgc
    43741 acagcgcctt tcagtacatc gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg
    43801 cccagaactt tcagcagttt gtcgttcggg atagtgatca gagagtccac atgcttggac
    43861 agttcagtga tcccctgctc ggcgaatgcc atacgcttct tgccttcaaa gttgaaaggc
    43921 ttagtgacga cagcaacggt caggataccc aaatcttttg ccacttcagc gacgactggt
    43981 gctgcacctg taccggtacc accacccata cccgcagcaa taaagaccat gtctgcacct
    44041 tccagcgccg cacgcaatgc atcgcgatcc tcatcagccg cattgcggcc aacttctgga
    44101 ttagcgccag cgcccagtcc tttggtgata ccgctaccga tttgaatcgt ctgtccaacc
    44161 gctgttttac gcagcgcttg tgcatcggta tttaccgcga agaattcaac accttcaata
    44221 cgctcgcgca ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc gatgacttta
    44281 atcaccgcgt cattggtaag ttccattggt tcaaacatag tttctctccg atttgtgcct
    44341 gtcgcctgag gccgtaatca tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa
    44401 ctattgagtc gcttgatcca cgagccaact gatgctgtaa cacgtttttc tacttcagct
    44461 tcaccgttaa gatgtgactc tttcccatag tgaagcaatc ccaccgccgt cgaataatac
    44521 ggctcctgag cataatccgt taaaccggta atgttcagcg gcgcgccgat acgcacttgc
    44581 gtatgaaaca cgcgctgagc acaggctgca agaccttcga tctgcgctgc gccaccggtt
    44641 aatacaatgc ctgccgccag gtgatgttta accccttgtt ggcgaagctt ttcctgcaac
    44701 tgcaatatct cttcgttgac caggttgagc aactcggtat agcgcggctc gatcacctct
    44761 gccagggtct gacgttgcag actccgcggc ggacgaccgc ctacgctcgg cacttccacg
    44821 ctctcatctt ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg
    44881 gcgtcgcttg gcggcgtgcc aaaggcgtaa gcgatatcac tggtcacgac attgccagca
    44941 taaggaatta ccttagtgtg gcgtaatgcc ccaccggtat aaacggcgat atccattgta
    45001 ccaccaccga tatcgacgac gcagacaccc agttcacgtt catcttccgt caataccgaa
    45061 taacttgatg ccagtccggc aaatatcagt tggtcaactt tcagcccaca acgttcaacc
    45121 gctttgacga tgtttttcgc catatcgttg tgacatgtga tcaggtgcac ttttgcctgc
    45181 atccgcacac ccgaaagtcc taccggattc ttgatccctt cctgatagtc aatcgcatac
    45241 tcttgcggga tcacatgcag cacacgatgc tcatcgcgca cacgcaccga tttcgcggta
    45301 tggacgacgt tttccacatc ttcttgcgtc acttcttctt cagaaatagg caccatacca
    45361 atttcattct ggcagctgat gtgcttacca gaaagcgcca gatataccga agagatctga
    45421 caatctgcca tcaattctgc ctggtcaatg gcgcgttgta cgcacttgac cacggattcg
    45481 aggtcgttca ccccgccttt atccatacca cgtgacgggc agctgcccac gccaatgata
    45541 ttgaccatac cgtcgggcag aacttcccct actaaagcgg caaccttcgc ggtaccaatt
    45601 tccagtccta ctaccagttt tctgtccgtc gccttgatca ttgttgttct gcctgtgcct
    45661 gattttgttg ctgagtagat tcctctggcg gcaagggcgc ccagcctact gccgctccag
    45721 agtcataacg caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta
    45781 aaaccggata aagttctaca aagcgagcca aacgtttcat cgtatcgccc cggccaagat
    45841 tgagcttaat atcgttattc agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt
    45901 ccttcagagt aaatctgtcc tttgccagca tctgccccat ttcgcgatag ccctgcaaca
    45961 cttcattagc gctgccttcc gggccataca gcattggaag cacctgcttg ctggtgcgat
    46021 ctggcggcac gctgaaggtg tttccttccg cgtctaccat gtgttgatca ttccaccgcg
    46081 caatcggcac atattcaacc agatgaatct tcaattcatc aggccactgc tttctgacgc
    46141 tcacctgctt aatccacggc aggcgttgtt ctatttgcgt ctggatgatg ttgacatcct
    46201 gggtcataaa ggtacccggc tcacccaatg ccaggatcga ctgccggata tcgtcattac
    46261 gcgtgtaatg gcgttcaccg gtcaacacca gctttgagag cggcaggcgt tgcgcatctt
    46321 ccatccagct caacacgacc cagccgctca ccaacactgt cgttaaaacg gtcagcagga
    46381 aaaggatccc cgccagacgc gttccattat tgcggcgaga agaaacctct tcttcgctgt
    46441 ttcgcgtgtt cagagcagcc tgcgacatat tagtccgcca gttccagaat tcgtactacc
    46501 aactgcgaga agctcatacc tgcctgacgt gccgccatcg gcaccaggct gtggctggtc
    46561 atacccggtg aggtattggc ttccagcaga taaaactgtc catcactgtc cagcataacg
    46621 tcaatacgtc cccatccttt gcaacctaac gtcgtccatg ctttcagcac taatgcctgc
    46681 aaattggcct cttgtgacgc tttcagacct gcggggcaga aatactgtgt ctcatcagag
    46741 agatacttcg cctcataatc atagaaggtt ccggacggtt gaatacgtat tgacggtaaa
    46801 atttcttcac cgagtatcgc aaccgtgaac tccggcccac ttagccattt ttcaatcaat
    46861 acttcttcat cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt ttctgctact
    46921 acttttgaca ttcccacact ggaaccttcg cggctcggct taacgataac cggcaaaccc
    46981 agagcagaaa tttctgctaa ctgcttatcg ctcaggcttt tttcaaactc tgcgcgggtt
    47041 aacgctaccc acggcgcgac cggtaaaccg gcaccttgcc acagaagttt gctgcgtagt
    47101 ttatctattg aaagcgcaga tgccatcact ccgctgccgg tataaggcaa gcccatcagc
    47161 tcgagcatgc cctgcagcgt accatcttca ccgccgcgac cgtgtagcgc gataaacact
    47221 ttctgaaaac ccatcgactt cagttgcgtc acgtcgactt ctttcgggtc gacaggatac
    47281 gcatcaatac cgccttcacg cagcccggct aacaccgctg cgccagaatt cagagaaact
    47341 tcccgctcag cggaagtccc gcccaacagg accgcgattt tatcagtcat gttgttcttc
    47401 ctccggagtt tgcggcttca gtttgatttc agctaaagaa cgggcaattt ttccgatatt
    47461 accagccccc tgaacgagaa tcaggtcgtt accggttaat accggtgcca gcatctcggc
    47521 tacctgcgca gggtccggca ccagaatggg atcaattttc ccacgtccac gaattgtgcg
    47581 acacagcgaa cggctgtccg ctcccggaat tggcgcttcg ccagccggat acacttccag
    47641 catcaacagg gtatcaacct gcgtcagcac attggcgaaa tcatcataca ggtcgcgcgt
    47701 acgggtaaaa cggtgcggct gaaacagcat taccaggttt ttatccggcc agcctgcgcg
    47761 cgccgcttta atggtggcgt caacttccgt cgggtggtgg ccgtagtcat cgaccagcat
    47821 tgccgtaccg cttttaccat tcactggctc cagcgggaat tcaccgagga aatcaaaacg
    47881 gcgaccagtc ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt caattccctc
    47941 ttccgtagca accgcaaccg cagctgcggc gttcagcgcg ttatgacggc ctggcgcatt
    48001 cagggtgacg cgcatcggct ctttgtcctg acgcagcagc gtaaagtgcc cctgcgggcc
    48061 aatctgctga taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt aagttgtggt
    48121 ctgacgtccc acacgcggta acaattcgcg gatcaccgga tcatcaacac acatcaccgc
    48181 acgaccgtaa aaaggcaggt tgtgcagaaa attaataaag gtctgtttta aattctcaaa
    48241 gtcgccctgg taggtatcca tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg
    48301 ttgcagatgc aggaacgatg catcactctc atctgcttcg gcaatcaggt accgaccatg
    48361 ccccaaacgc gcatgaaccc ccgccgcttt taccagcccg ccgttaacga aggttgggtc
    48421 gagccccgct tctgcgtaga tgctggaaac catcgcggta gttgtcgttt tgccgtgcgt
    48481 tcctgcaatg gcgatgccat gacgaaaacg cattaactca gccagcattt cggcacgacg
    48541 gatcaccgga atacgcgctt catgagctgc gacaatttcc gggttgtcgg cagaaatggc
    48601 gctggaaaca acgaccacgc tggcatcacg tacgttttcc gggcgatggt tgaaataaat
    48661 cgtcgcaccc agattcagta actgctgcgt gaccggattt ggcgctaaat cggaaccact
    48721 gatctgataa ccttcattgg ccagaacttc ggcaataccg cccataccgg caccaccaat
    48781 gccgacaaaa tgtatgtgcc gaacgcgacg catttcgggc acgatggaac gcagttttgc
    48841 caattgttgt gtattcattc tttacgccat taacttctta aattcatacg atgcaaaagg
    48901 catcgctaca attacgcccg ggcagcccgg ctcacttcat ttgccactcg ctcggtggca
    48961 tccggaatgg atgcagcgcg ggcgcgttct gccatggtta ataaggtttc tcgcgaccac
    49021 ccggccaggg tgttggcgac agcatccacg ctaagctgtg gctgctcgat aattttggct
    49081 gcgcccgctt tttccagcgg tagcgcattc cagtattgct ggcggtcttt atgttgaaac
    49141 ggcacaaaca acgccggtag tcctgccgcg gcgatttcac tcacagttaa agcaccggag
    49201 cggcaaacga cgacatccgc ccacgcatac gccgccgcca tatcatcaat aaattcagtc
    49261 actttatgct gcggttgccc cgcttcggca tacgcctgtt caacggattg ttgcgaacct
    49321 ttgccgctct gatgccagat agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt
    49381 gtctggttaa gaatgcgtgc gccctgggaa ccacccacta ccaacacacg aaccggacct
    49441 tcacgtccag ccaaacgttg ctgcggcaac ggcagcgcca gcacatcggt acgcaccggg
    49501 ttacccacca cttccgcatt agggaaagcg cctggaaacg cctgcatcac tttggtagca
    49561 atcttcgcca gccatttatt ggttaagccc gcaataccgt tttgttcatg aagtacaacc
    49621 ggaatgccta acgaccacgc ggccagacca cctggacctg atacatagcc gcccataccg
    49681 agtaccacgt caggtttgta cgctttcata atcgcccgcg cctgacgcca gccgttgaag
    49741 atacgcagcg gcgcagctat cagtgctttt ataccttttc cacgcaaacc agagatacga
    49801 atgaaatcaa tttcgatgcc atgttttggc actaagtccg cttccatacg gtcggcagtc
    49861 cccagccagc gaacttgcca accctgagcc attagatagt gcgcaaccgc cagtcccggg
    49921 aatacatgtc caccggttcc gcctgccatc accattaatc gctttccttg accactcatc
    49981 gtgaacctcg tacaaacgcc tgcgctttct ccagacgcgt ttcataatca atacgcaaca
    50041 gcatcatgat ggctgtcgac ataatcagta agctcgaacc accgtaactg attagcggca
    50101 atgtcagacc tttggtcggt aacatccccg ccgccgcgcc tacgttaacc agcgcctgga
    50161 agctaaacca gatgccaata gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta
    50221 atgctttgcg gccaatcgac atcgcacgaa aagcgacgaa gaataccatt aaaagtgcta
    50281 gtaccacacc gacataacct aattcttcgc cgataatggc gaaaataaag tcagtgtgcg
    50341 cttccggcag atactccagt ttttgtaccg aattacctaa accttgcccc caaagttcgc
    50401 cgcgaccaaa cgccatcagc gattgcgtta actgatagcc gctgccaaag ggatcttccc
    50461 acgggttcca gaatgcggta acacggcgga tacggtacgg ttcggcgagg atcagcaaca
    50521 caaccgctga aatgcccata ccgataatgg caatgaactg ccacaatttc gctcccgcca
    50581 ggaacaacat cgccagcgta gtcacaaaca acaccaccac cgtaccaagg tctggctgtg
    50641 ccagcagtaa cactgccaac accagaatca cgcccatcgg tttcaggaag ccgcgcaggt
    50701 tattacgtac ttcgtcgcct ttacgcacca gatagttggc gatatagcaa aacagcgaca
    50761 gttttgtcag ctccgcaggc tggatacgca gcaaaccgag atcgatccaa cgcgatgccc
    50821 ctttaacagc gctacctact accagcacga tcatcagcag gatgatagat ccgagcagca
    50881 tcgtggcact gtagcgttgc cagaactcca tcggcagacg cagcgtaatg atcgccagaa
    50941 taaacgccaa aatcagatag acaccatcac gcttcgcgaa gaagaacgga tcgttggtta
    51001 agcgttgccc tatgggcatt gacgccgagg tcaccatgat aaagccaatc gccgcgaggc
    51061 cgaaggtcag ccacagtaag gtgcgatcgt acatgatcag gctgtcggta tctttttccc
    51121 gcgagcccat cacccagccc tttagcgccg tggagatcca gaccaagata ctgaatcctg
    51181 gcaggcgcgg cattttcagg cgagggagag ataaacgcat caacctaact ccttcgccag
    51241 acgggcaaac tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc
    51301 cggggagagc agaaccatat cgcccggctg aacacgcgga gccagcaagc gcatagcctg
    51361 ttccatagtt tcggtttgtt ctgccacttc cgggcgtagc gccgccagct gcgcgccgtc
    51421 acgaccgaaa caatacagac gtacgttatc gccattcagg taacgcgcca gtgggctaaa
    51481 gtccgccgat ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta cgtgcaggcc
    51541 attcagcgcc gcttccgtac tgccgacgtt ggtcgctttt gaatcgttaa tccagcgcac
    51601 accgttatgc tccagcacca cttcaaagcg atgcggcaga ccggtgaagg tggttaacgc
    51661 tttcaggctg ctggcgcgcg gtaaccctgc ggcatctgcc agcgccagcg ccgccagcgc
    51721 attggtgtag ttatgctgcc cggaaagttt catctctttc acattcagca ctttctcgcc
    51781 tttaacccgc agccaggttt cgccctgctg atgattcagg tgatagtcac ccatgttgac
    51841 gccaaagctg acgcagcgtt catccgcacc gcgaatcggc attgttaagg catcatcagc
    51901 attaaccacg caaactttcg cgttttcgta aatgcgcagt tttgctgcac gatactgttg
    51961 taaaccaaac gggtagcgat ccatatgatc ttcagtcacg ttcagaatgg tcgctgctac
    52021 cgcctgtaag ctggaggtgg tttccagctg gaagctcgac agttccagca cgtacagttc
    52081 acactcatca tccagtagca tcaacgcagg caggccaata ttgccaccca caccaacgtt
    52141 aacccccgcc gctttcgcca tttcacccac tagcgtggtg accgtgcttt tgccgttaga
    52201 accggtaatc gccacaatcg gtgcttgtgc ttcgcgacaa aacagctcga tatcgccaac
    52261 gatttcgatt ccggcatcag cggcagcgct taaggatgga tgcgccagtg caataccggg
    52321 actggcgaca atcagatctg ccgccatcaa ccattcatca ttcagactgc ccgtgtggcg
    52381 ttctacggct tcgggtaatt tatccaggct aggcggtgtc atacgcgtat ccataacgcg
    52441 cggcgtcaca ccgcgagcga ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc
    52501 gataatgaca acatttttac cctggtaatc agccatgatt aacgtacctt cagcgttgcc
    52561 agaccaatca ggaccagcat cagcgaaata atccagaaac gcacaatgac gcgcggttcc
    52621 ggccagcctt tcagttcata gtggtgatga atcggtgcca tgcggaaaat acgttgtccg
    52681 cgcagtttaa aggagccgac ctgcaggatg acagaaagcg tttctaccac gaacacgccc
    52741 cccataatca ccagcaggaa ttcctgacgt agcagtacgg cgataatgcc taacgcacca
    52801 cctaacgcca gcgaaccgac atcgcccata aagacctgcg ccggataggt gttaaaccac
    52861 aggaagccca gtcctgcccc gactatcgcg gtacagacaa taaccagttc cccggcgtgt
    52921 cgcagatacg gtatgtgtaa atagctggca aagttcatgt tgccggtcgc ccacgccacc
    52981 agcgcaaaac caccggcgac aaatacggtc ggcataattg ccagaccgtc aagaccatcg
    53041 gtcaggttta ccgcgttgcc agtacccaca atgacgaagt aagccagcag aatgtagaac
    53101 agccccagtt gcggcatcac atctttaaag aatgggacca ccagctgcgt tgcgggcgtg
    53161 tctttgccgg caaggtacaa agcgaaagca acacccagcg caatgaccga catccagaaa
    53221 tatttccaac gagcgatcaa ccctttggta tctttacgca ccactttgcg ataatcatca
    53281 acaaagccaa taacaccgta acctaccagc accaccaaca cgcaccagac gtacggattg
    53341 gacgggtaag cccacagcag cacggagatc acaatcgctg tcaggatcat aatcccgccc
    53401 atggtcggcg taccgcgctt gctgaagtgt gattcaggac cgtcgttacg cactacctga
    53461 ccaaaggaaa gtttttgcaa atgagcaatc atgcgcgggc ccatccacaa tgagatgaac
    53521 agcgcggtca gcaggctgac gatggcgcga aacgtcagat aggaaaagac gttaaagccg
    53581 gaataatatt tgaccaaatg ttcggccagc caaactaaca tgtcccattc tcctgtaaag
    53641 cgcgtactac ctcttccatg gcagcactac gtgaaccctt aactaaaatc gtaattacct
    53701 gttgctcagc aatcagtgat ttaagacgcg taattaacgc agttttatca gcaaaatgtt
    53761 cgccaacgcc gctggcggtg ctgatagcat ggctttgttt acccatgctt aacacgcggt
    53821 caataccagc agcttttgcc gcctcgccca cctgtacatg gcagtcttcg ctttcagcgc
    53881 ccagttccgc catatcgccc accaccagca cgcggtagcc cggcatttca gccagtacct
    53941 gaacagctgc ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc agcaactggt
    54001 tttctgccag ttggatgggg aacagacggc caggaacagc tttcagattt gccagccccg
    54061 ctttgatagc atcaagcgtt gcgcccacgg acatggagag cgcagcggct gccagcgcat
    54121 tcgcaatatt gtgacgcccc ggcaacggca gcagaacatc gacgctaccg gttggggttt
    54181 gtagggtaaa ttccataccg tgcgaggtca catggatatt ggtggcggtg aaatcgctgt
    54241 tggcggcatt gggtgagaaa cgccacactt tgcgtgagcc aattacgctc tgccagttca
    54301 gccagtcgtt gttgtcggcg ttcataatgg cgataccgtt ttccggcagg ccgctaaaga
    54361 tttcaccttt cgctttcgcg acacccgcaa gcgagccaaa accttccaga tgcgccgctg
    54421 ccaggttgtt gaccagcgcc gcttccgggc gagtcagact cacagtcctg gcaatctcgc
    54481 cctgatggtt cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca
    54541 gcgtcatcgg tacaccgatg tcgttgttga gattgcctgc cgtataaagc gtgttgccgc
    54601 actggcttaa aatcgccgcc gtcatctctt taacggaggt tttgccggag gaccccgtca
    54661 gagcaaccac gcgcgccgga acttgctggc gaacccatgc agccagttca ccaaacgcca
    54721 gacgcgtatc cttgacgatt aactgcggca ggtcgatgtc cagcggacgg ctaaccagta
    54781 gtgcgcctgc gccgccagct ttcgcctggt cggcaaaatc gtgggcatca aaacgttcac
    54841 ctttcagggc aacaaacagg cagcccggcg tcagttttcg ggtatcagtg gttacagcat
    54901 caagggtgat atctgcacct tgcagttcac cgttgagaat atcggtaagt tggctaaggg
    54961 ttacgctaat catgcaatcc cccccagcag acgcgccacc gtgacgcgat cggagtagtc
    55021 cagacgctga ttgccaacaa tctggtaatc ctcatggcct ttgcccgcga ccagtaccac
    55081 atcattctct ttagcctgca taacggcgca agtcaccgct tcagcacggc cttccatcac
    55141 tttggcatgt ccggcatcta acattcccgc cagaatatcg ttgatgatgg cacgcggttc
    55201 ttcggtacgc gggttatcgt ccgtcaccac cgccacgtca gcaaactctt cggcaattgc
    55261 gcccatcagt ggacgcttac ctttatcgcg atcgccacca cagccaaaga cgcaccacag
    55321 cttgcccgca cagtgcaggc gcgccgcctg taaggctttt tccagtgcat ccggcgtatg
    55381 cgcgtaatcc accaccaccg tcggtttgcc tggcgcagtg aacacttcca tacgtccgca
    55441 aaccggttgc agacgcgcgg cggttttcag cagatcagcc agtggatagc ccagcgccag
    55501 cagtgttgcc agcgcgagca acaggttgct gacgttaaaa gcgcccatca gatggctttc
    55561 aatttcgcca tcgccccagc ttgaactaaa gcgaatggtc gcgccgctgt cgtgatagtt
    55621 cacttcggtc gctttcaacc agcgtccgtg acagttcgga ttgatatgat cttccattga
    55681 taccgcaacc gcgtccggca gttttgccag ccagcggcgg cccacttcat cgtcggcgtt
    55741 aataatcgcc tgaccgcaat gatgttcaga ataaagcagc catttcgcag cttcgtagtg
    55801 ttccatatca ccatgataat caaggtgatc gcggcttaag ttggtaaaga ccgacgccgg
    55861 aaatttcaat gccgccacac ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca
    55921 aaaagtcgcg ccctgatcca ccagccccgc cagctcatgc tgaacatcaa ctgccgaacc
    55981 ggtagtattt tctgtcggga tcactttccc cagcagaccg ttaccaacgg tgcccattac
    56041 cgcgctggtt tcgccaagca gttggctcca ctgcgccaga agctgggtcg tcgtggtttt
    56101 gccgttggtg cccgttacgc ctacgagacg taaattgtca gagggttcat ggtaaaagcg
    56161 gcccgccagt gcagataaac gctcgttgag ctggctgaga tagatgaccg gtacgccgtg
    56221 catttcacgg atttcaccgt cggtcgcctc atctttcgcc tctgcaataa tggcagccac
    56281 accttgcgct atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc
    56341 tacaaagaga tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg
    56401 cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg
    56461 tcccctcgcc ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct
    56521 cgatgttcat ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg
    56581 cgccgccgta gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg
    56641 gctgactcgc aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt
    56701 ccggcccgac ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg
    56761 ccttcacgcc gccgccgcct ggtagcgcca cgctttccat catatgcacc acggtgcgaa
    56821 caatggattc cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca
    56881 gtgggcgata aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg
    56941 ttaccattag cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt
    57001 tttgaggata taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc
    57061 caaaacgtga gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa
    57121 caccgacgtt actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca
    57181 cgtctttgat ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc
    57241 gcaccacgcc acgttgcaac gcggtcatca ccaccatcgg tttaaccgtt gagcccggtt
    57301 caaacacgtc ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt
    57361 tagggttgta tgacgggctg ttagccatcg ccagcacttc acctgtgttg acatccacca
    57421 gcacggcgct accagattca gccttgttaa aggccaccgc attgttcagt tcgcgataaa
    57481 ccagcgcctg caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag
    57541 tagaagaaat atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg
    57601 gctgcccggt aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga
    57661 cgttagtaaa gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact
    57721 cttcacgcag atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagagt
    57781 ttacctgacg cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa
    57841 gctgatccag cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga
    57901 taccgccagc gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca
    57961 ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa
    58021 cgcgaagaga acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc
    58081 acgctacgcg tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa
    58141 aacgccaact gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg
    58201 ctgctttcat gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat
    58261 caacatgctg catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga
    58321 gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca
    58381 ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacggtca
    58441 ccgccgtcaa aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat
    58501 cgtcaccgat aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt
    58561 tgcttagagc ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa tacgcagaac
    58621 tgaactacgg gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag
    58681 tgctcgcagc tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc
    58741 cggaacttgc ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag
    58801 cgagtggaag ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt
    58861 tagcgcctgc tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt
    58921 acgggtcgcg ggatgtttaa acttatcttt caccggcgtt gcagcagcca cgacttccgc
    58981 cagttctttg gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat
    59041 gcgtttggca aaacgctctt caccataggt tttcaatacc caggcgatat cggcttcttc
    59101 tgcggtttgt agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag
    59161 cggaccatcg cgcataaagg aaaagccgcg ttcagcatca tcaagttgcg gtgaagagac
    59221 gccaagatcg aggagaatgc cgtcgatctt gccaataaga tcgcgctcgg caacgtattc
    59281 gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc
    59341 aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg
    59401 cgagaggatc agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc
    59461 aggacggata ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt
    59521 ttccatcatt ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact
    59581 gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa
    59641 acttgttgaa ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta
    59701 ctggcgcgat taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca
    59761 gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct
    59821 cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg
    59881 tgcaaaccat ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg
    59941 ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac
    60001 cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa
    60061 agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata
    60121 tcagcgttgt ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa
    60181 gaaaaaatcc ctcatcgtca tcactgtttt aaaatgactc actattttcc acagcaaata
    60241 taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc
    60301 cttgttacgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgt
    60361 ttgttaaatc ggcaattcat cgccgcatgt aacaaaataa tgcaaaaacg cgctgtcgat
    60421 atcacttaat tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaaccat
    60481 ttatcaataa tagtcaggaa tagtcttatt tactttaa
//
LOCUS       DAJTYI010000008        57497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYI010000008 DAJTYI010000000
VERSION     DAJTYI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..115)
                     /locus_tag="OO564_000517"
     CDS             complement(<1..115)
                     /locus_tag="OO564_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6651771.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDE"
     gene            270..842
                     /gene="yeiP"
                     /locus_tag="OO564_000518"
     CDS             270..842
                     /gene="yeiP"
                     /locus_tag="OO564_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6651772.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1065..2531
                     /locus_tag="OO564_000519"
     CDS             1065..2531
                     /locus_tag="OO564_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6651773.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2649..3635
                     /gene="yeiR"
                     /locus_tag="OO564_000520"
     CDS             2649..3635
                     /gene="yeiR"
                     /locus_tag="OO564_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6651774.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3674..4387
                     /gene="lpxT"
                     /locus_tag="OO564_000521"
     CDS             3674..4387
                     /gene="lpxT"
                     /locus_tag="OO564_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6651775.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4799..5365
                     /gene="mepS"
                     /locus_tag="OO564_000522"
     CDS             4799..5365
                     /gene="mepS"
                     /locus_tag="OO564_000522"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6651776.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5546..7102
                     /locus_tag="OO564_000523"
     CDS             5546..7102
                     /locus_tag="OO564_000523"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6651777.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7184..8998
                     /locus_tag="OO564_000524"
     CDS             7184..8998
                     /locus_tag="OO564_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6651778.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8999..10093
                     /locus_tag="OO564_000525"
     CDS             8999..10093
                     /locus_tag="OO564_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6651779.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10093..11118
                     /locus_tag="OO564_000526"
     CDS             10093..11118
                     /locus_tag="OO564_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6651780.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11120..12709
                     /gene="yejF"
                     /locus_tag="OO564_000527"
     CDS             11120..12709
                     /gene="yejF"
                     /locus_tag="OO564_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6651781.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12713..13057)
                     /locus_tag="OO564_000528"
     CDS             complement(12713..13057)
                     /locus_tag="OO564_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651782.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13390..14580)
                     /gene="bcr"
                     /locus_tag="OO564_000529"
     CDS             complement(13390..14580)
                     /gene="bcr"
                     /locus_tag="OO564_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6651783.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPSLPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14608..15303)
                     /gene="rsuA"
                     /locus_tag="OO564_000530"
     CDS             complement(14608..15303)
                     /gene="rsuA"
                     /locus_tag="OO564_000530"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6651784.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15453..17213
                     /locus_tag="OO564_000531"
     CDS             15453..17213
                     /locus_tag="OO564_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6651785.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17338..17622
                     /gene="rplY"
                     /locus_tag="OO564_000532"
     CDS             17338..17622
                     /gene="rplY"
                     /locus_tag="OO564_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6651786.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17761..18768)
                     /gene="yejK"
                     /locus_tag="OO564_000533"
     CDS             complement(17761..18768)
                     /gene="yejK"
                     /locus_tag="OO564_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6651787.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18950..19177
                     /locus_tag="OO564_000534"
     CDS             18950..19177
                     /locus_tag="OO564_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6651788.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19197..20957
                     /gene="pbgA"
                     /locus_tag="OO564_000535"
     CDS             19197..20957
                     /gene="pbgA"
                     /locus_tag="OO564_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6651789.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21032..21108
                     /locus_tag="OO564_000536"
     tRNA            21032..21108
                     /locus_tag="OO564_000536"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21066..21068,aa:Pro,seq:ggg)
     gene            complement(21211..23802)
                     /gene="yejO"
                     /locus_tag="OO564_000537"
     CDS             complement(21211..23802)
                     /gene="yejO"
                     /locus_tag="OO564_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6651790.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24122..24769
                     /gene="narP"
                     /locus_tag="OO564_000538"
     CDS             24122..24769
                     /gene="narP"
                     /locus_tag="OO564_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6651791.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24980..26032)
                     /gene="ccmH"
                     /locus_tag="OO564_000539"
     CDS             complement(24980..26032)
                     /gene="ccmH"
                     /locus_tag="OO564_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6651792.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26029..26586)
                     /gene="dsbE"
                     /locus_tag="OO564_000540"
     CDS             complement(26029..26586)
                     /gene="dsbE"
                     /locus_tag="OO564_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6651793.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26583..28526)
                     /gene="ccmF"
                     /locus_tag="OO564_000541"
     CDS             complement(26583..28526)
                     /gene="ccmF"
                     /locus_tag="OO564_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6651794.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28523..29002)
                     /gene="ccmE"
                     /locus_tag="OO564_000542"
     CDS             complement(28523..29002)
                     /gene="ccmE"
                     /locus_tag="OO564_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6651795.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28999..29208)
                     /gene="ccmD"
                     /locus_tag="OO564_000543"
     CDS             complement(28999..29208)
                     /gene="ccmD"
                     /locus_tag="OO564_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6651796.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29205..29942)
                     /gene="ccmC"
                     /locus_tag="OO564_000544"
     CDS             complement(29205..29942)
                     /gene="ccmC"
                     /locus_tag="OO564_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6651797.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29984..30646)
                     /gene="ccmB"
                     /locus_tag="OO564_000545"
     CDS             complement(29984..30646)
                     /gene="ccmB"
                     /locus_tag="OO564_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6651798.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30643..31260)
                     /gene="ccmA"
                     /locus_tag="OO564_000546"
     CDS             complement(30643..31260)
                     /gene="ccmA"
                     /locus_tag="OO564_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6651799.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31279..31881)
                     /gene="napC"
                     /locus_tag="OO564_000547"
     CDS             complement(31279..31881)
                     /gene="napC"
                     /locus_tag="OO564_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6651800.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31891..32340)
                     /gene="napB"
                     /locus_tag="OO564_000548"
     CDS             complement(31891..32340)
                     /gene="napB"
                     /locus_tag="OO564_000548"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6651801.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32337..33200)
                     /gene="napH"
                     /locus_tag="OO564_000549"
     CDS             complement(32337..33200)
                     /gene="napH"
                     /locus_tag="OO564_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6651802.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33187..33882)
                     /gene="napG"
                     /locus_tag="OO564_000550"
     CDS             complement(33187..33882)
                     /gene="napG"
                     /locus_tag="OO564_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6651803.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33889..36375)
                     /gene="napA"
                     /locus_tag="OO564_000551"
     CDS             complement(33889..36375)
                     /gene="napA"
                     /locus_tag="OO564_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6651804.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36372..36635)
                     /gene="napD"
                     /locus_tag="OO564_000552"
     CDS             complement(36372..36635)
                     /gene="napD"
                     /locus_tag="OO564_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6651805.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36625..37119)
                     /gene="napF"
                     /locus_tag="OO564_000553"
     CDS             complement(36625..37119)
                     /gene="napF"
                     /locus_tag="OO564_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6651806.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37527..38015
                     /gene="eco"
                     /locus_tag="OO564_000554"
     CDS             37527..38015
                     /gene="eco"
                     /locus_tag="OO564_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6651807.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLESWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38165..39196)
                     /locus_tag="OO564_000555"
     CDS             complement(38165..39196)
                     /locus_tag="OO564_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6651808.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39430..39789)
                     /locus_tag="OO564_000556"
                     /pseudo
     CDS             complement(39430..39789)
                     /locus_tag="OO564_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40007..41650)
                     /gene="yojI"
                     /locus_tag="OO564_000557"
     CDS             complement(40007..41650)
                     /gene="yojI"
                     /locus_tag="OO564_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6651809.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41726..42320)
                     /gene="alkB"
                     /locus_tag="OO564_000558"
                     /pseudo
     CDS             complement(41726..42320)
                     /gene="alkB"
                     /locus_tag="OO564_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42320..43384)
                     /gene="ada"
                     /locus_tag="OO564_000559"
     CDS             complement(42320..43384)
                     /gene="ada"
                     /locus_tag="OO564_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6651810.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43458..44513)
                     /gene="apbE"
                     /locus_tag="OO564_000560"
     CDS             complement(43458..44513)
                     /gene="apbE"
                     /locus_tag="OO564_000560"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6651811.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44625..45746)
                     /gene="ompC"
                     /locus_tag="OO564_000561"
     CDS             complement(44625..45746)
                     /gene="ompC"
                     /locus_tag="OO564_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6651812.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46484..49156
                     /gene="rcsD"
                     /locus_tag="OO564_000562"
     CDS             46484..49156
                     /gene="rcsD"
                     /locus_tag="OO564_000562"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6651813.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49173..49823
                     /gene="rcsB"
                     /locus_tag="OO564_000563"
     CDS             49173..49823
                     /gene="rcsB"
                     /locus_tag="OO564_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6651814.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50023..52872)
                     /gene="rcsC"
                     /locus_tag="OO564_000564"
     CDS             complement(50023..52872)
                     /gene="rcsC"
                     /locus_tag="OO564_000564"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6651815.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(53147..53923)
                     /locus_tag="OO564_000565"
     CDS             complement(53147..53923)
                     /locus_tag="OO564_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6651816.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53928..55577)
                     /locus_tag="OO564_000566"
     CDS             complement(53928..55577)
                     /locus_tag="OO564_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6651817.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55578..57497)
                     /locus_tag="OO564_000567"
                     /pseudo
     CDS             complement(55578..57497)
                     /locus_tag="OO564_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14060 a        15526 c        14123 g        13788 t
ORIGIN      
        1 gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc atctggcata ccgggaatag
       61 ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat tccatacgtt
      121 gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt ttttgcacca
      181 gcaaaagtgc gaataccact tgcccgaaag gcccatcgcg agtactttgt cgcgatattt
      241 ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt aaaaaaggta
      301 tggtactgaa ttacaacggc aaactgctgc tggctaagga tattgatatt cagtcgccca
      361 ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga
      421 aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg acccgccgtt
      481 acgttgactt ctcctatgtc gatggcaacg aatatgtctt tatggataaa gaagactata
      541 ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg
      601 gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc
      661 agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg
      721 cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa
      781 gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact
      841 aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac
      901 caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga
      961 tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataact
     1021 ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct
     1081 ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac
     1141 gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc
     1201 gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg
     1261 atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg
     1321 cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg
     1381 attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga
     1441 cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc
     1501 cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg
     1561 tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg ctctcctgcg
     1621 acaatattcc cgacaatggc catgtggtga aaaacgcggt gctgagaatg gcagaaaaac
     1681 gttcgccaga actcgccggg tggataaaag aacacgtcag ttttccggga accatggtcg
     1741 accgcattgt tccggctgca accgacgaat cactggcaga aatcagccag catctggggg
     1801 taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt gaagataact
     1861 tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc
     1921 catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttctc gcttatctgg
     1981 gttacctctc aggattcgcc tatatcagtg attgcatgca ggatcgcgta tttcgctatg
     2041 ctgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt
     2101 taacacaata tgcggataag ttaattgcac gttttgctaa tccgtcgctg aaacataaga
     2161 cctggcaaat cgcgatggat ggcagccaga aattaccgca acgtatgctg gcaggtattc
     2221 gcatacatct ggggcgtgaa acggactggt cgttgctggc attaggcgtt gctggctgga
     2281 tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta
     2341 gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc
     2401 tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca
     2461 tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc
     2521 tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc
     2581 ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttacca gagtattgcg
     2641 gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt
     2701 cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg
     2761 aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag
     2821 agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata
     2881 ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc
     2941 cgaaacagct cctcgatctg ttaaccgcac cagtctatga accgtggata gatctgcgcg
     3001 ccaccttgtg cattctcgat ccacgcctgc tgctggacga aaaaagcgcc agcaatgaaa
     3061 acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga
     3121 cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat
     3181 taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt
     3241 tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag
     3301 cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat
     3361 atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg
     3421 aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc
     3481 tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac
     3541 cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgcc ttgcagagcg
     3601 cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat
     3661 aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggccc
     3721 cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc
     3781 ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt tatggctggt
     3841 tgcattgact aacaatcgcg ccttcgacgg ttgttcactg ctggcgatgg gtatgttgat
     3901 gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct
     3961 ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa
     4021 acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt
     4081 tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat
     4141 tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat
     4201 ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat
     4261 tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag
     4321 tgatcgatta atcacctttt ttgacaaatc actaccagga aaaaacaaac atttccaaaa
     4381 caaataactc acagtaatta acatcatcag ggttattttt atagtgagga taatcctgat
     4441 gatgcgcacc gtgctttcat ctatcgaacg caaaaatcat tctctaagta aatgaatgga
     4501 ttgcatgcgt ttcactcaat tgtactttaa ttgaccaacc ccgcttatta actttctgta
     4561 tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa
     4621 tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta
     4681 gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgtcg
     4741 cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat
     4801 ggtcaaatct caaccgattt tgagatatat cttgcgcggg attcccgcga ttgcagtagc
     4861 ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg
     4921 tgcaatgggt agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt
     4981 tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg
     5041 ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggtttcg tacagcgtac
     5101 attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg
     5161 taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc
     5221 aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag
     5281 cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc
     5341 acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga
     5401 cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat
     5461 gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc
     5521 gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct
     5581 cctgcttacc tgcattgtag caggcgtgat gattgcgata ctggttagct gccttcagtt
     5641 tttagtggcc aggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa
     5701 gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac
     5761 cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa
     5821 tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga
     5881 gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa acgtcgatat
     5941 ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg
     6001 caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc
     6061 tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac
     6121 tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt
     6181 ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac
     6241 atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct
     6301 gctttgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca tgaccgccat
     6361 caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgggt
     6421 aacgggcctg gaagtactgc tacgctggcg gcatcctgta gcgggagaaa ttcccccgga
     6481 tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt
     6541 tgagttaatt gcccgcgatg ccgcagaatt agaaaaagta ctgccggtag gcgtcaaatt
     6601 tggcattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa
     6661 actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga
     6721 tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcacttgg tcggcgtaga
     6781 aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac
     6841 gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac
     6901 ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc tgacagttac
     6961 tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg ttaatttcat
     7021 gcaaggctac tggattagtc gcccgttacc gctggacgat tttgttcgct ggctgaagaa
     7081 accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat
     7141 gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg tgcgcatact
     7201 gctgctgttt atcgctctgt tcaccttcgg tgcgcaggcg caggctatca aggaaagcta
     7261 tacctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt
     7321 gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggta ccttcgataa
     7381 tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac
     7441 gttatttacg acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagtgc
     7501 acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca
     7561 tgatggttct cccattactg cccgcgatgt agagtttact tttcaaaaat ttctgaccga
     7621 aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcgccgtt
     7681 aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc tgttttcgct
     7741 gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc
     7801 tccgcttgcc agtggtccgt accacgttac gtcctggaaa atggggcaaa atattgtcta
     7861 ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgtggac gctggcattt
     7921 cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc
     7981 aggtgccttt gatttgcgta tggaaaacga cgctaaaaac tgggccacgc gttataccgg
     8041 taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga
     8101 tacgcgctgg ctggcgttta atatccaacg tgcggtattc agcgatcgcc gggtgcggga
     8161 agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaacgcctg
     8221 gagtcgcacg aacagttatt ttcaaaatac cgaatacgcg gccagaaatt accccgacgc
     8281 cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca
     8341 aatctaccag ccgccggtat ctaaaggcga tggctacgat cgtgacaacc tgttaaaagc
     8401 cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg ttaatgccac
     8461 aacgggtcag ccgctcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt
     8521 attgccgttc cagcacagcc tgcaacggct gggtatcaat atggacattc gcaaggtgga
     8581 taactcgcaa atcactaacc gcatgcgcaa tcgcgactat gacatgatgc cgcgcctatg
     8641 gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa
     8701 ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga tcaaccaaat
     8761 tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt
     8821 attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg
     8881 gtgggataaa ttctcccaac ccgctgtacg ccctgtttac agcctaggta tcgatacctg
     8941 gtggtatgac gttaataaag cggccaaact gccgtcagcc agacaacagg gagagtagat
     9001 gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acgttatggg ctattatcac
     9061 tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg ccatcgccgc
     9121 cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag
     9181 ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat taccgtggcg gacgcggatt
     9241 agatccagaa gtgatcgctg agatcactca tcgctacggt ttcgataagc cgatccacga
     9301 acgttacttc aaaatgctct gggactacat ccgctttgat tttggtgata gcctgtttcg
     9361 cagcgcgtcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt
     9421 gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta
     9481 taatgggagt cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc
     9541 ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct
     9601 gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat
     9661 caccgattat ctgtggcata tcacgctacc ggtgctggcg acagtgattg gtggctttgc
     9721 ggcgctgacc atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt
     9781 gaccgcgcgt gcgaaagggg taagtgagaa aaacattctc tggaaacata tgttccgcaa
     9841 cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg
     9901 ctcgctgctg attgaggtga tgttttcact caatggtctt ggcttactgg gctacgaagc
     9961 gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg
    10021 cctgctgctg aatattgtca gtgatatcag ctatacgctg gttgatccgc gtatagattt
    10081 tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgt
    10141 cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg
    10201 tgttctgaac ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg cagttggtat
    10261 ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct
    10321 gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca
    10381 ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca
    10441 ccctcccggc aaaactggct gggaacggat gcaaacggcg gcgatgtgct ggcacgcatt
    10501 ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg
    10561 atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctggggg
    10621 caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc
    10681 agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg
    10741 agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga ctacattcgt
    10801 gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat
    10861 gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg
    10921 acctcgctcg atttcctcgg cttcggtcta ccgctcggtt caccgtcact cggtgaactg
    10981 ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc cttcttgtcg
    11041 gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat
    11101 cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt tgtcggtggg
    11161 ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc
    11221 tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc
    11281 aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttca
    11341 tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat
    11401 cgccatgatt tttcaggaac cgatgctgtc gttaaatcca ttgcataccc tggaaaaaca
    11461 gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gtggcgaaat
    11521 tcttaactgc cttgatcgcg tcggtatccg ccaggcggca aaacggctga cagattatcc
    11581 gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg
    11641 accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca
    11701 gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac
    11761 tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg
    11821 ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca
    11881 aaagctactc aacagtgaac cgtcaggcga tccggtaccg ttgccagaac ctgcctcaac
    11941 gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg
    12001 cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacggg cgggtgaaac
    12061 actgggttta gtgggcgagt ctggttccgg gaaaagcacg acgggactgg cgctgctgcg
    12121 actgattacc tctcagggca gcatcgtctt tgacggtcag ccactgcaag atttaaatcg
    12181 ccgccagctg ttacctactc gtcatcgtat tcaggtggta tttcaggatc caaactcctc
    12241 gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc
    12301 gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt
    12361 agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat
    12421 tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg aaccgacatc
    12481 atcactcgac aaaacggtac aggcgcaaat attgacgctt ttgaaatcat tgcaacaaaa
    12541 gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca
    12601 tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt
    12661 tgccacaccg cagcaggagt atacgcgtca gttactggcg ttgagctgac gcttaaaaag
    12721 gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg
    12781 cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac tccaacaccg
    12841 aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat
    12901 tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg cacggtccat
    12961 tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga tagttctgcg
    13021 gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg aagtacattc
    13081 gcttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat
    13141 cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt aatatctgcg
    13201 ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt
    13261 aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg
    13321 gaaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttacc ttcctcgttg
    13381 tgcaatagat caccgttttt tcggccgact ggcgtacaga cagaagagaa tggagctggt
    13441 tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag
    13501 caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt
    13561 tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga
    13621 cacgcagccc acaaacaccg caacgccaac caccagcgac cagaatccca gccccagcag
    13681 cgcactgatg accatccacg ctgccataat aaattgtatc cacaaccccg agcggaacat
    13741 atttaacgcg ccaatgcggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa
    13801 aacaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt taatttcaat
    13861 ataaacaaac ggtccggcgc ttaagaatga gaacatcccg gcaaagctga aaccactggc
    13921 aagcatgtag ctcaggacac gtttatggcg gaacagcgcc gcaaagttac caatagtggt
    13981 acgaatgtga aatggctgac gacgctccgg tggtaaggtt tctttaatca ggaagaaaat
    14041 cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat gccagctcag
    14101 ccacaccagc acccagccgc caactatcgg tgccatcagc agtgcaatgg tcgtcaccag
    14161 catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcac gcatcaaagc
    14221 gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag
    14281 ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtccc
    14341 gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc
    14401 caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg ccgatacgcc
    14461 aaactgcgct gaaattaccg gtagcgaggg cagatacata tcaatcgaca gcggcatcaa
    14521 catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac
    14581 aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt cttcagttaa
    14641 cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa tacgttcacg
    14701 atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct gatgataacg
    14761 cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttcaagca ccgccggctt
    14821 agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc
    14881 agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc gcggagaagt
    14941 aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg tatcgatatc
    15001 cagacgtcct gcggcgtgca gtttccacgc taccggttca tccagaaaat agagcaccgt
    15061 cgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta
    15121 acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcagggag
    15181 cagtttgaac gctgcattac gaacgatttc gccatcgacg gtgacacgat tgccgcggat
    15241 ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg
    15301 catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact
    15361 gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagaa ccgtggcata
    15421 ctattagcag aataatctac ctacgcgaaa ccatgatttt tacactccgc ccatatcagc
    15481 aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg
    15541 tgctgcccac cggcgcaggt aaaagcctgg tgatagcgga actggcacgg ctggcgcgtg
    15601 gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaagtatc
    15661 aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg
    15721 gtaaagtggt atttggcagc gttcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg
    15781 aattttcgct gttgattgtc gatgaatgcc accgtattgg tgacgatgaa gagagccagt
    15841 atcagcaaat cctcactcac ctgactaaag tgaatcccca cttacgcctg ctggggctga
    15901 ctgccacgcc ttttcgattg ggcaaaggct ggatctacca gttccattat cacggcatgg
    15961 tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg ctgcgttata
    16021 tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta gtgcaatacg
    16081 atttcagccg cttgcaggca cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg
    16141 agctgaaaaa acagcaacgc attacgccac atatcatcag ccagatcatg gagttcgccg
    16201 aaaagcgcaa aggggtaatg attttcgccg ccaccgttga acacgcaaaa gagattgtgg
    16261 gattactgcc tgccgaagat gctgcactga ttactggcga cccccccggc gctgagcgcg
    16321 atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac gtcgcagtac
    16381 tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat
    16441 cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg ggcaagactg
    16501 attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta
    16561 caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg
    16621 ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgcc
    16681 gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc
    16741 gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg
    16801 aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag
    16861 atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg
    16921 aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc
    16981 tgcaaacgcc cgcccagcgt accgccttcg agcagctttt tatccgcccg catacgcgca
    17041 caccgggcat ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttac
    17101 tgcgacatcc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa
    17161 aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt
    17221 tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc
    17281 agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg
    17341 tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt
    17401 gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcaccgct ggctatcgag
    17461 ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag cgaagttctg
    17521 accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg
    17581 tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa taagttgtaa
    17641 aaaaccccgc tccggcgggg ttttttgtat ctgcagatta tgcctgatta cggtattgct
    17701 attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga
    17761 ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt
    17821 aatggtcaag gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc
    17881 aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct
    17941 acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa
    18001 gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga tctcttcgcc
    18061 tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc
    18121 tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg
    18181 cgctttcgcg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt
    18241 gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat tggtttccca
    18301 ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg
    18361 gttgatgtcg agattttcgt taacgcgcat actgctcagg ttgctcagaa ctgccaccag
    18421 cagatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa
    18481 aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa
    18541 gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa
    18601 caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac
    18661 gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc
    18721 acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc
    18781 tttaagaccg ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc
    18841 attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc
    18901 gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat
    18961 ttcccgctac agtaatgaac aggttgaaca attgctcgca gagctgctca acgtactgga
    19021 aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct
    19081 tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc
    19141 cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg
    19201 taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt
    19261 ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg
    19321 actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca
    19381 gcttcctggt gttcgccacc tacttgctga tcctcttccc gctgaccttt atcgtcggct
    19441 cccagaggct gatgaggttt ttgtccgtca ttctggcgac ggcgggaatg acgctattac
    19501 tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagt
    19561 tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg
    19621 tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca
    19681 gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta
    19741 tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg atcaccatgc
    19801 agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgtttcctt gagaagcatg
    19861 gcctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat ccagacaccg
    19921 tttccgtaca gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg
    19981 tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc
    20041 tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta
    20101 cagacaacgg catctttggc ctgttctatg gcatctcgcc gagctatatg gacggcattc
    20161 tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg
    20221 ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt
    20281 tctcgatgcc gagcgtacgc acccaatctg acgatcagac cgccacgcag tggatcaact
    20341 ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtttct ttcaatggca
    20401 ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcggcaggca
    20461 atgtcgatga ccagatcaaa cgcgtgctca atgcactgcg tgattctggc aaactggaca
    20521 atacggtggt gattatcact gccggtcggg gtattccact gagcgaagag gaagaaacct
    20581 ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg
    20641 cgcagcgtat taatgctctg actgatcata ccgatctgat gacgacgctg atgcaacgcc
    20701 tgctgcatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc
    20761 aacgccgtca ttactgggtt actgctgcgg ataacgatac gctggcaatt accaccccga
    20821 aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa
    20881 gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca gacgagaagc
    20941 gttttatcgc taactgatta attataaatc agttagcgaa atatcttact tgcaatcggt
    21001 gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcttg gtagcgcacc
    21061 gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga
    21121 aaaaaccaac ccttacggtt ggttttttta tatctgcaat taattcgata aacagaccgt
    21181 gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccacac ccgcctgagt
    21241 attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat gaccgcttaa
    21301 cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccactggttc cagccacatc
    21361 attattaaat ttgccatcgt cattaacttt cacctggtta gaatcggcgt attcctgacg
    21421 cacggcggct ttcagccagg gttccagcgt cgtaccgttt tgcaggtcca tgtgatagct
    21481 taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc
    21541 gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca gatagggtct
    21601 aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac ccgcgccgtt
    21661 actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga tggtgttggc
    21721 aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct cccaaccggc
    21781 ataagccccc agggtatagc tatcgatatt accttttccg ccgcgatcaa aaccaatatc
    21841 agaatgagag taaccaaaga acaagccgcg aattgtactg ctttcttcac gggagaaacg
    21901 gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt
    21961 ggtcacgttg ttgcgggtgt taattgccga actccacatc accgtatcgt aactaacgcc
    22021 ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata ccagcggttg
    22081 tgcggctgcc atgttcagca catcggtggt tgaaggggta atttgcgcgc gattctctgc
    22141 gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac cgatatcaac
    22201 aacgcctccg gcattgccca acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt
    22261 aaggctatct cctgctgccg ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc
    22321 tgttgcctga ccggtgacgt tgagctgatc gctctgatga ttggccatat cggtacgcat
    22381 ataaaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat tttccgcact
    22441 tgccgtcaca gatgaacgag tcactggctc agccatcata atcacatttg cgccggacat
    22501 cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat cggcaatgat
    22561 ggtccatgcg ctattttcga gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt
    22621 tctaaaagaa gcgccctcag aaaccactac cttacccacc aacatagcgg gtgcataatt
    22681 tatttgcggc ttcatgacct caagaatgcc atcattccct ctgactgaca cgttaaccat
    22741 tgtgccagcc cagacattag cgcggccatt gttgattacc atattttcag agatggcttt
    22801 tgaggaatac gtgatacttc cattctggtc tgatcgaccg agatcatata ccgcatttgc
    22861 ctgcactttc gtaccagtat cctttcccag cgattgcata gtggcatgct catcaaggat
    22921 agtgtcaata gcctgcgtgt cctccataac aatgagcccg gaaccatcat ccagttcata
    22981 attttttgac acaccatcct ttatactaaa ttggccttta ctgtttgtac cactcacttc
    23041 cagcgcattc gttgagacaa ttaatttccc gcctgctttt ttatcaacgc cagttgcagt
    23101 cccgccatcc ataacctcca gtaagccgcc actatccaca gtagtattat aagcgaagtc
    23161 attttcttca actcgcaaac ttccgccgtt ttccaacaac atattattag caataccatt
    23221 cttgatatcg aactgaccga gacggtttgt tccgaatacc tccatggccc gcgtggttgt
    23281 tttaatagca ccgcctgctt tctgatctac cgcaagcgca aatcccccct ctttaacctc
    23341 cagtagtcct cccttttcga tagcggcaaa aacggtcccc ccatcttttt caacgattaa
    23401 gcgcccgcct ttaatcgtgg tatcattcgc tgggccataa acctgtagca caccattatt
    23461 gagggtagtc tggtttgccg cgccacccat ttgaacaatt tgataaccat catttaatac
    23521 tgagtattct gcggtaccat tcatttcaat gtattgatac cctccgttaa tttcagtatt
    23581 actacttaca ccaaattctt ttatatgctg ttctccgcca ggattgattt tcgtattatt
    23641 ggcggtccca taaaccagct ggatatcttt ttccaggata acaccatcaa ctgtctcacc
    23701 atactcaact gttgatgcca atgctatggg tgaatataac gctgtagcca ccagaacaga
    23761 tattgcggaa cgacaaagag aaacagaacc agattgatgc attgagcttt catcctatga
    23821 aattaattgc agttaaaagc attgggtaca gaaaataccc atagctccat acccggagtc
    23881 agtttttaaa aactgtttaa agaaatgcac aagtattgtg attgattttt tagttgtttt
    23941 tcttgatgag aagctgatgc aaaattccgt ctttataatg aaaatgatgc caaagcgaac
    24001 gacaaggttg tagttttcac tacatgtcca tacataaaat ggggtaacat tcacgcgcct
    24061 ggtagcgtta ccaacgctac gctcaaacat aatgattcta ataaaacctc aggagactac
    24121 tatgcctgaa gcaacgcctt ttcaggtgat gattgtggat gatcatccac ttatgcgacg
    24181 cggtgttcgt cagttactgg agcttgatcc tggctttgaa gtggtcgccg aagcgggcga
    24241 cggcgcgagc gcgatcgatc tggcgaatag actggatatc gacgtgatct tgctggatct
    24301 caatatgaaa ggtatgagtg gcctggatac cctcaatgcc ttgcgcaggg atggcgttac
    24361 cgcgcaaatt attatcctga ccgtatccga tgcctccagc gatgtctttg cgctgataga
    24421 cgcaggcgca gacggttatc tgttgaaaga cagcgacccg gaagtattgc tggaagcgat
    24481 tcgtaccgga gcgaaaggca acaaagtctt tagcgaacgc gtcaatcagt acttacgtga
    24541 acgtgaaatg tttggcgcgg aagaagatcc cttcagcgtg ctgacggagc gcgagctgga
    24601 tgttctgcac gagctggcac aggggctgtc aaataaacag attgcatcgg tgttgaatat
    24661 ttccgagcag acagtaaaag tacatattcg caatctgctg cgtaaactca atgtccgctc
    24721 acgcgtggcg gccaccattc tgttcctgca acaacgcggg gcacaataaa aatagcccga
    24781 tggatttatc atcgggctga gatttatgac aaacgcaaaa ctgcctgatg cgctacgctt
    24841 atcaggccta cgtggatcga tcaatttatt gaatttacac aattttgtag gccggataag
    24901 gcgttcacgc cgcatccggc ataaactaag cgcactttgt catcagtcta gcccgatggc
    24961 atcaccatcg ggcctctttt tatttactct cctgcggcga caaatgttgc atcgcctgcg
    25021 cgatactacg ttcaatcacc gcacggcgag tatcgttggc aggtaagagt ttcaacatca
    25081 tctcccacgc ggcaacggct tcgccaaatc gctgctgctc aaaggcatta aacgcataca
    25141 tgcttagcac acggatattg ctatggtccg ttctcaccag ctgacgtagc agttcaccgc
    25201 cgaggcggtt gtcgttggga tcagatgaac gagtcaacgc ttcagcgtat cccagtgcgg
    25261 catcgctgtt cttcggatcg aggcgatacg cagtagcgta tgcatcggtg gcgatactgg
    25321 cgttacccag cgccatgcca acgcggccca acataatcca gccttctata tctcccggat
    25381 ttttttgcag ttgagtacgc atccccagcg caaggcgcga catctcttct tcgttgagcg
    25441 gatcggcttt cgggtccagc gccctgtcca gcaacgccgg agcctgtgcc gtggcctgct
    25501 gccagatttt cacctgctga taattgccag tctggtagta gctgacgcca gccacaatta
    25561 acgccaccac aatacccggc agataaacaa catatccggc acgcttacct tccggcacgc
    25621 tttgttcagg aaacgcttcc ggcaccacgc gtacccgacg ccgcgagcgg gcgtatatga
    25681 cccaaccgcc aatgccaata gccactactg gcagcaccca cagcagcacg gtcagcggcg
    25741 ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc caccatataa tcgacaatct
    25801 cttttttact tttaccttcc tgcatcagtt catacacttt ctgacgcagg tcggtggcaa
    25861 tcatcgagtt ggaatcggca atgctgttgt tctggcattt cgggcagcgc agttcttcag
    25921 tgagctgacg gaactgttgt tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg
    25981 ccagcgctga gccggagatc atcagcatca gcacgcccaa taaaaacctc attgtgcggc
    26041 ctccttactg tatttctccc acagcggctt gatctcttct tcccagacgc gaggattgag
    26101 atcgcccgca tggcgatagc gaatgatacc gttgccgtca ataaggaagg tttctggcgc
    26161 gccatagaca ccgagatcca gccctaacat gccgtcgcca tcaaacaggc ttagcgcgta
    26221 aggattgccc agctctttca gccagctgat tgccttctgg cgatcgtctt tatagttcat
    26281 gccgaccacg cgaatgccct gcgctgaaag ctgattcaga tattgatgtt ccgcacggca
    26341 ggtcggacac caagtcgccc agacgttaag cagtactggt ttgccctgag tcagcacatc
    26401 cgcctgataa aactgccccg gattgtccag tgattcgaga cgaaacttcg gcacaggctt
    26461 gccaatgagc gccgattcca gattggtcgg atcatcccct tcggcattac gcgccagctg
    26521 ccacagcagc gccgcggcaa tcgccaggaa gataatcaac ggaattaaca atactttgcg
    26581 cttcatacgg cctccggcgc agttttttgc ggactcacgc gcttacgata gcgaggatca
    26641 aacagacaca gcagtccgcc caacgccatc atcagcccgc ccgcccaaat ccagcgaaca
    26701 aatggtttgt agtaaagacg cacggcccac gcgccgtttt ccagctcttc accgagcgcg
    26761 gcgtacaggt cacgcgtgat gccgccgtca attgccgctt cggtcatcat cgacccggca
    26821 gtgttgtaat aacgtttttc cgcatacagc accgtttccg gtttaccctc tcgcgttacg
    26881 ccaatagtcg ccacaccgcc gcgccagttc gggccagtca cctctttgac atcacggaag
    26941 gtgaagcgat attcatgaat atcgacgcta tcgccggact tcatgcgcac atcacgctca
    27001 acgctataat tctggctaaa ggcaatgcca acaattgtca ctgccagccc caggtgagcc
    27061 gccaccatcc cccaataact gaaggtggtt ttcgtgccgc gtgaaatacg cagcgcagct
    27121 tccgcagttg ccagcaccgc aatccagcag gccattgcca ggccgagcac cgtcatcgcc
    27181 acaactttgc tttcgaacag ccacggcaac agcagagaca gcaccagcgt agagatgaag
    27241 gcgataatca ataaattgcg gattttacgt gggcgatcgc gcccccagcg caccagagga
    27301 ccgacaccaa gcagtagcgc aaacggcacc atcagccagg taaacatggt gttgaagaac
    27361 ggttcgccaa tcgaaatact gcccagtcca agttgcttgt gcaccagcgg cagcagcgtc
    27421 cccagcaaca ccaccagcat cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat
    27481 tcccgcgacc acagcgcatt gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac
    27541 agcagcagcg aaccgccaat caccagcacc ataaaggcga ggataaacat accgcgcgcc
    27601 ggatcagacg caaacgcgtg taccgatacc agcacgccgg aacgcacgag gaaagtcccc
    27661 aacagacaca acgagaaggc actgattgcc agcagtaatg tccacgcttt gaagctggcg
    27721 cgttgttcag tgaccgccag tgagtgcatc agtgcagtcc ccaccagcca cggcataaac
    27781 gaggcgtttt ctaccggatc ccagaaccac cagccacccc agccgagttc gtaataggcc
    27841 catgcggaac cgagcacaat gccgagcgtc aggaaaatcc acgccgccag cgtccacggg
    27901 cgagtaaaac gcgcataagt gctgtccaga cgcccgctca gcaaagaagc aatggcaaaa
    27961 gcaaacgcca ccgagaaacc cacgtacccc atataaagca gaggcggatg gaagatcagc
    28021 cccggatcct gtaacagcgg gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg
    28081 cgtgagaacg ggttagaggt aaagagaatg aacagcaaaa agccgacgct gaccatcccc
    28141 attatcgcca gtacacgggc cacaatatcc agcggaatac gctgactaaa aatcgccacc
    28201 gcaaaggtcc agccgctcat cagcagcacc cacagcagaa gcgaaccttc atgcgcgccc
    28261 caggtagccg ccacgcgata ccacaccgga agctgggtat tggagttgct ggcaacatag
    28321 gtgacggtaa agtcgttgac cacgaaagca ttgaccagca ccagaaatgc gccagccaca
    28381 gacataaaca gcagccaggc aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc
    28441 gccacgcccc atagcggata cacggacagc agcagcgcaa tccctaacgc caagcacagc
    28501 agcccgtttc caatttccgg catcatgatg ctgggtcctt ataaacactc gccgggcgac
    28561 ggtggttagc ttccatcgct ttctcaactt ctggcggcgt gtagttttcg tcgtgtttcg
    28621 ccagcacttc tttcgcgagg atatgattgc ctttttccag ctcgccctgc accacaacgc
    28681 cctgcccttc acggaacaga tccggcaaaa tgccttcgta agagacatcc actgagcctt
    28741 cagcatcgta aatggtgaag gtcactttca gcgaattggg atcgcgctgc acgctacccg
    28801 gcatcaccat cccgccaacg cgcagacgct gaccgacttc cggcatctgc tgagtttcac
    28861 gcttgccgta gagaatttcc cccggcgtat aaaagagatc gatattcgag cgcagcgcat
    28921 atagcaccag accgatagtc agcgccagcc ctgccaacac ggcacaggca atccacaagc
    28981 ggtttttacg gcgaatattc atgcagcctc ctgctgttgc gcagcacgta aacgcgcctc
    29041 acgcgcccgc tgttgcgcca caccacgcag aattgcgcga tgttgcatca ccgagtgcac
    29101 gaccaaaacc accagcggaa taacggtcat caccaccgcc agccagacaa aaaaggcgta
    29161 accgcccatt gcgaaaaatt cattccagga agcaaatgca ggggtcattt acggcctctt
    29221 ttcagtatca gttcactcac ccacggacgg cgtttttcca tcagcaaaat caaattacgc
    29281 atccgcatca gcgtcagcgt ggcagacagg agcaggaagc caaaaatcga ccagcgcagc
    29341 ggcgaacgca tcgccggatc gatactttgc tgcatccgcg ttgatccctg atgcagggtg
    29401 ttccaccact ccacggagta atgaataatc ggcagattca ccacgccaat cagcaccagg
    29461 atacctgccg cacggcccgc cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca
    29521 cccacataca aaaacagcag caccagttca gaagtcagac gtgcatccca tacccaccag
    29581 gtgccccaca tcggttttcc ccatgcagag ccggtaacca gggcaataaa ggtaaacacg
    29641 gcaccaatgg gggccatcgc cgccaccgcc aggttggcca ttttcatctg ccagacaagg
    29701 ccaataaacg ccgccaccgc cattgatgca taaatgccca tcgaccagat cgccgcaggc
    29761 acatgcaggt agataatgcg gtagctattt ccctgctgat aatcagccgg agcaaagccg
    29821 aatccccaga tccagccgac ggtaagcacg accacactgg caattgccag ccacggtata
    29881 aaccagccac agatttgata cagccgtggt gggatcgcca gttgatgcag tgttttccac
    29941 atagtttcga taccagactc gaacaaaaat cagtaatcca gcgttattga atgctgattc
    30001 gtaacgctgc cgccgtcgca aaaggactta atgtcgcggt gcctgccagc aacgcgccta
    30061 aaattgccag atacccatca acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg
    30121 caaagatgag taatgggata gtcagcggta acaccagtat gctgagcagc acaccaccgc
    30181 gcttaagtcc cactgtcagc gccacgcccg gtgcaccgag aaagccaagc gtaggcgttc
    30241 ccagcagcag cgtcagcgcc atcacttgcc agccataaac atccattccc agtagcattg
    30301 ctaccagtgg cgaaaggatg agtaacggca gaccggttac catccagtgc gccatcacct
    30361 tcgccagcac aacggcgggc aagggtaacg gcaacaacat caattgttca agactgccgt
    30421 cctgcaaatc gtcacggaac agtcgttcca gcgccagcaa ggatgaaagc agcgcagcaa
    30481 cccagataat gcccggtgca atacgcgcca gcagttgcgg ctccggaccg atactgagcg
    30541 gaaaaagggt aattacaatc aggaagaacc acagcgggtt ggcgatttcg gcgctatggc
    30601 gaaacgctac gcgcagttca agacggaaaa tacgccagaa catcatgcgg ccctcgtttg
    30661 cgtcagtgaa atgcggcgaa ttttactttc agcaacgttg agcggctggt gggtagtcag
    30721 aatcacaatc cccccctgct ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc
    30781 gttaacgtca atcgcggtaa aaggctcgtc gaggatccat aatgtggcac gggtcaacca
    30841 cagacgcgct aaagcgacgc ggcgttgttg cccggcagaa agttgattta ccggaatatc
    30901 ttcgaatccg gcaagcccgg cctgcgccaa ggcttccaga cattgtgcgg catcgccatc
    30961 gcgatgataa aagtgcagat tttctaacgc cgtcagccgg gttttgatcc ccggctgatg
    31021 gcctatccat aacaggtttt gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg
    31081 ccagagaacc tcgccagcgt cagggcgaga caaccccgtc agcaaacgga gaagcgttgt
    31141 cttccccgcg ccgttgctac cggtgatttg tacccactct cctgcgttca gcgtaaatga
    31201 caagccacta aataaggttc gttcatcccg ctcacaaagt acctctctgg cttcaagcat
    31261 acccacgcaa taaccctgtt aaaaacctgg ctcgacttca cgcatatcgg gcagcttgtg
    31321 cgcgatccct ttatggcaat caatacaggt ttgcccatct ttcaccgcct ggtcatgcat
    31381 cttcgcggca accgatttct gggcggttgt atccatatac tcgaagttgt gacagttacg
    31441 gcactcctgc gagttattgt ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg
    31501 atgagcttca aatttctgcg gcgtgtcaat aacgccaaaa attttaccat acagctcttt
    31561 acttgctttg agcttgcgta tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg
    31621 acaggtcgca cggacgccgc tacggttgtt gtagtgcacg gaatccatgt attcctgata
    31681 caccgtgttg cgcatttcgt ggcagctaat gcagaactct tcggtattgg ctttttccat
    31741 cccggtgtta aagccccccc agaagacgat gccgccaaca aaaccgatca acagcagcgt
    31801 ccccagcgcc agatggctgg gggtacgcca ccatttccac aggcgcttaa tcagaccagg
    31861 cttacggtca gaatttccca taataacctc ttatttcccg taaccttttg atggggtaaa
    31921 ggtattcccc acgattggcg cggtatcggc ctgcggtacg tgacattgca gacagaaata
    31981 acgacgcgga gccacttccg cgcccacttt gccgtcgctg tccataaagt gagtaggact
    32041 gatacgcggc gcgccagtgg tgcgatagct ttcgacaccg tggcactgca agcagcgatt
    32101 ggtattggtc gttacctgat aaccttcaac gctatgcggg atcattggcg gctgattcac
    32161 atagttcagc ggcatccgat cctgctcttt tggcatccga atggcccctt cctgcgtccc
    32221 ggatacttcc ggcgactggc taaaatcgac gccattagcc gcccaaaccg cgccgcttac
    32281 caccagggcc agcatcgccg tccattgaca cagcgctttc ttcaggtcat ggcttttcat
    32341 gatttcgctc ccgaactcca tcgtgtagtt attgtaaata catcctcaga acagacatcc
    32401 acgcagcgac cgcacgtcat gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc
    32461 agcaccgggg cacgtagcac atgcggttcc gggcaaacat gaaaacaatc catacagcgg
    32521 ttacatttct gccgatcgct ggccgcaacg gtaatcacac ctttgctacc cagcacgcca
    32581 tacagcgcgc caaccggaca aatgtgcccg caccagccgt gttcaacgac cagtagatca
    32641 aataaaaaca gcgcgagaat aagcagcgcg ccgctgccga agcccattac caggctacgc
    32701 cccatcagag aaacggggtt gatccattcc caaatgagcg tgccagttaa ggctgatccc
    32761 accagaatga ccaccaacag cacgtagcga atgtggcgag ggatcgtcgc agactgattg
    32821 aggtcaaagc gcctgcgtaa ccagttcgct aagtcggtaa tcgggttcag cgggcagacc
    32881 cagctgcaaa ataatcgctt tcccgccaag gcgtagagca cggtgataat caccgcaccg
    32941 gtcaacgcca ccgtggcagg cagatgacca ctggcaagac tttgcagcgt catcagcgga
    33001 tcggtcagcg gaacggtgtc gaataacagg ctactgctgt agttgccgtg caagatccac
    33061 acaccaaacc atggaccgct caaaaacatc cccagcacga agaactgaca aaggcgacgt
    33121 aacaccagcc aacggtgact gcgccaccag ccttttttct ccagcgcctc gcgcccggcg
    33181 tcacgtttac gatttgccat tgttcccctc cagccagccg aagcggtaat ggtgacctaa
    33241 ctcccctttc gccagtgaca gcggtaacac cttgattgcc ggttgttcca gcacgcatac
    33301 tttttcgcac ttaccgcaac cggtacaggc gtcgctgtga accgtcggca gaaagcgggc
    33361 gtgcttaccg gtacgcgtgt tgcgctccag ctccaaggtg atggcctcat caattttcgg
    33421 gcattcgcga taacaaacat cgcagcgcag cccctgaaag ttgagacagt tttcctggtc
    33481 caccagtacc gccagcccca tccgcgcgtc gtcgatcgat tcaatctcac gatccagcgc
    33541 accgcttggg cacactttgg cgcacggaat gtcctcacac atttcgcaag gaatatcccg
    33601 tgcgacaaaa tatggcgtgc ccgccgacag accagaggcc agcgtcgcca gttttaaggt
    33661 gtcgtaaggg caagcctgaa cacactgacc gcaacgcaca caggcactgg caaaggcgtt
    33721 ctcgtttatg gctccgggcg ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg
    33781 taaccccagc gccacaccca cggcagccag cccgcctgct gtgcgaacga catcgcgcag
    33841 aaagcggcgg cgaccatttt gaggtttcgc tgaccgggac ataatggctt acaccttctc
    33901 cagtttgacc gcgcacttct tgaagtccgt ctctttcgag agcggatcgg tcgcatccag
    33961 cgtcagtttg ttaaccagct gtgcggcgtc gaagaacggc atgtacacca ggccctgtgg
    34021 cggacggtta cgaccgcgcg tttcaacaat cgagatcact tcgccacggc gagaaaccac
    34081 tttcactttg tcgccacggc gcagatcgcg cgctttcgca tccagcgggt gaataaacaa
    34141 gaccgcttcc gggaaggcgc ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg
    34201 ccagtgctcc agaacgcgtc cggtagagag ccacaggtcg tactcttcat ccggtgcttc
    34261 cgccgccggt tcgaacggca gtgcgaagat caccgctttg ccatccggtt taccgtagaa
    34321 cttatagcct tcgcccgctt tcacgtacgg gtcgttacct tcgctgtaac gccactgtgt
    34381 ttctttacca ttcaccaccg gccagcgcag accgcgcgct ttgtggtagt catcgaacgg
    34441 tgccagatcg tgaccgtgac cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg
    34501 cagatagaag cccagctcgc gggattcatc gttcagctga tcttccgcca gttcggatac
    34561 cgggaatttg ctcacttcgg gggtggcata cagaacttcg tacagcgttt tgccacgcag
    34621 ttccggtttc ttcgccagca gctcttccgg ccatacttct tcagttttga agcggcggga
    34681 gaactggact aactgccaga gatccgattt cgcttcgcct ggcgcctgta cctgctgacg
    34741 ccagaactga gtacggcgtt cggcgttacc gtaagcgccc tctttctcta cccacattgc
    34801 ggtcggcagg atcaagtcgg cggccagcgc actgactgtc ggatacggat cggagacgat
    34861 gatgaagttg cgcggatcgc gccagcccgg catacgctct tcattgatgt tcggcccggc
    34921 ctgcatgttg ttggtacaca tggtccagta aacattcagc ttgccgtctt tcagcgcacg
    34981 gtcttgtgcc accgcatgaa gaccgatttt cgccggaatg gtgccgctcg ggatattcca
    35041 cttcttctca cagatatcac gatgtttctc gttagttacc accatgtccg caggcagacg
    35101 gtgagcaaag gtgcccactt cacgcgcagt accacacgca gaaggttgcc cggtcaggga
    35161 gaacggaccg caacccggct gggaaatttt gccggtcagc aggtgcaggt tgtagaccag
    35221 gttgttggcc cacacaccac gggtatgctg gttgaagccc atcgtccagt aggagatgac
    35281 tttcttgttc ggatcggcat acagctgcgc cagttgttct aactggtctt tcggcacgcc
    35341 ggtcatttca gcggtttttt ccagcgtata ctcagcaacg aaggctttgt aatcttcaaa
    35401 gctcatcggt tcggaggcgt cagaacccgg attcttcgct gctttttcca gcggatgggt
    35461 cggacgtaaa ccgtagccga tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa
    35521 gaagtcctga tttatcgcat tgttttgaat gatatagttg gcgatgtagt tcaggatcac
    35581 caggtcagat tgcggcgtaa agatgatgcc gttatccgcc agctcgaagc tacgatgctg
    35641 gtaggtagaa agcaccgcca cggtgacgtt ctggttagag agacgacggt tagtgatgcg
    35701 tgaccagagg atcgggtgca tctccgccat gttagcgccc cacagcacaa acgcgtcagc
    35761 ctgctcgatg tcgtcatagc agcccatcgg ctcatccata ccaaaggtac gcataaagcc
    35821 aactactgcc gacgccatac agtgacgtgc gttcgggtcg atgttgttcg aacggaagcc
    35881 cgctttgaac agcttggacg cggcataacc ttcccagata gtccactgac cagaaccgaa
    35941 cataccgatc gattccggcc ctttttcttt catggcggtt ttgaatttct cttccatcac
    36001 atcgaaagcc tgatcccagg tgattggggt aaattcgcct tctttgtcat atttaccgtt
    36061 tttcatacgc agcagcggct gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa
    36121 atagccctta atgcagttca ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc
    36181 caccacacgc ccctgctgcg ttccgaccag aacgccgcaa ccagtaccgc agaaacggca
    36241 cggcgcttta tcccatttaa tggcttcctg ctgaccaaca acggcgcggg caacgcccgg
    36301 cacgctgaga ccggcagccg ccgcagcggc cgcaacggcg ttagctttca taaagctacg
    36361 acgactgagt ttcatggtgt ttcctcacct tgctcttcct gctggtgata aaccagcgac
    36421 accgccagca cgccctctac gttgcgtact gactcaatgg tttggatcag cgtttcgctg
    36481 tcttctgctt ccaccaccac aatcaactga ccgctcggcg cgtcgctgac agcaacttca
    36541 cagccgggaa aggcgttcag ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc
    36601 acgaccaggc tgcaaacttg ccagttagtg tgcatggaga tactccgcag ttatggctga
    36661 taccggacag ctggctgcac atgctccaca tccgttgcaa agttggctat taagttgcgc
    36721 ctggtagatc ccggagagcg tcgggcgaaa gataattgcc attggctcac agctatcctg
    36781 acagcggcgg cattcaactg actgatacgc caggcacgcg tccccgatgg tgaactgcaa
    36841 atcccagacc ctggtgtggc gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc
    36901 gtaacagaag ctgcactcgt tatttttgaa attaacgctc ggatagccac ccgtgccgcg
    36961 ttgcagaatg ttgttttcac aagcattaat acaggcgtcg cagcgggtgc aatgggtcag
    37021 aaaatgagat tcatcaccgg accagggcgg acggataccg ttactggctt tgcgccagcg
    37081 accagtgagt atgccccgac gggatgcatc aatcttcaca ttgaccttcc atcattaacg
    37141 cgctctgaaa attgagagca accaaataga ccgcataatt aataagccat ttttatagcc
    37201 gctaagatat taaaggatgt gtcaaagatg cataccccga tcggggtaaa acctgtagca
    37261 ggatcaaaag agtggcggga agcgtggcaa aaacgggctt ttgctcacat ttcaaatggt
    37321 tataaacata tttatatagc gattaattca ccagagatat ttctgcttgt ttgctccctc
    37381 atcagaattt aacactaaaa gagcaggtaa aattgtctga atgttcttta agttattcat
    37441 aaagcaaatt aataaatctg atgaatatgt taaccttcag cgacatcatc ggtgaaaacc
    37501 tataaatgaa gaaggaaagc aaaaaaatga agaccattct acctgcagta ttgtttgccg
    37561 ctttcgctac cacttccgcc tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc
    37621 cttatccaca agctgaaaca ggcatgaagc gtcaggtgat tcagttaacc ccgcaagaag
    37681 atgaatctac cctgaaagta gaactgttaa tcggtcagac gctggaagtc gattgcaatt
    37741 tgcatcgtct cggcgggaag ctggaaagca aaacgctgga aagctggggc tatgattatt
    37801 atgtctttga taaagttagc tcgccggttt ccacgatgat ggcctgcccg gatggcaaga
    37861 aagagaagaa atttgtcacc gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca
    37921 agctgccgat cgtggtgtat acgccagaca atgtagatgt gaaataccgc gtctggaagg
    37981 cggaagagaa aattgacaac gcggaagttc gctaaactgt tgtgaagtac ggcaccccgt
    38041 tggttggata aggcggttac gccgcatcca acatctaacg cccgagccgg ttgcctgatg
    38101 cgacgctggc gcgtcttatc agtatacgcc acatccggca taccatgccg gatgtggcac
    38161 atcattacaa cgcaatatcc gccacttctt tttgaacggg ttgcggtttc aactgcggtt
    38221 tcggcgtact atctgctgct tgcggtttgt cgtatttcag ccccagcact tcgctggtgt
    38281 actgcaactc gcgttctgtt gccgctacat caccgttcag cttgcgtcca taagacggaa
    38341 cgatcgcttt caacgtagac tgccattgcg ggctggaaac gcgatcgcca aatacttttt
    38401 ccagcagatt caacataatc ggcgcggcgg ttgacgcccc tggcgatgcc cccagcagcg
    38461 ccgcaatggt cccttgctgg tcactgacca cttcggtacc cagacgcagt acgccacctt
    38521 tctcggcatc acgcttgata atctgcacac gctgccccgc ttgccacaaa cgccagtcct
    38581 cttttttcgc ttgcggatag tactctttca acgcttcaaa acgatcctct tcactcaaca
    38641 tcacctgact caccagatat ttcaccagat cgaaattatc cagcccgacg tgcatcatcg
    38701 gcatcacgtt agaggtggtg gtggaactca tcagatccca caatgaaccg tttttgagga
    38761 atttggttga gaaggtggca aatggcccaa acagcactac gcgtttaccg tccagaacac
    38821 gggtatcgat atgcggaacc gacatcggtg gtgcgccaac ggatgcttta ccgtaaactt
    38881 tcgccagatg gcgattaacc acgtctggat tttccgaaac aaggaactgt ccgcccaccg
    38941 ggaaaccggc gtagtctttc gcttccggaa tccccgattc ctgtaacagc ttcagcgccg
    39001 caccgcccgc gccgataaag acaaatttgg tgcggatgtt ctgtgcagtg ccgtttttca
    39061 gatcggcaac ggtaacggtc caggtgttat cgtcattacg ctttagggcg cggacttcgc
    39121 tgctgagttg cagcgagaag ttagatttct tctgtaagga agcaattaac tggcgggtga
    39181 tctcaccata gttcacatca gtgccaattt ctgttgcgga tcgcgccctt ccatcactaa
    39241 cggtgcccac tctttgatct gcgcgtgatc ttcagagtaa cgcataccgc gaaacagcga
    39301 gctttgttgc aacgcggcgt aacgggcgcg caggaaatta acgttatcct cgccccagac
    39361 aaagctcata tgcggaacgg tattgataaa tgaacgcgga gtacgcagca cgccgcgctc
    39421 gacctggtgc gcccagaact ggcgggaaat ctgaaatgct tcgttaatgg cgactgcttt
    39481 ttcaatactg atgctgccat cggcgttttg cggggtgtag ttcagttcca tcagtgcaga
    39541 atgcccggtt ccggcgttgt tccagccgtt cgaactctcc tgcgcgcccc cctccaggcg
    39601 ctccaccatg gtcatcgacc attcaggctc cagctcgcgt aaataggtcc ccaacgtggc
    39661 gctcataatg ccgccgccaa tcaacagtac atcagtttcc tgctcctcgg acgctttcgc
    39721 ttttgccgcc atcgaaacgg cattaagccc cacggccatc gagaagagca tggcagtcac
    39781 ttttttcatc ttgttaatgc cttactttta gtcgctttat tgcaggtgag atttgcgcgg
    39841 catcaacggt aacacttaag taacaacatt taaataatgt ttataaatta tattctaatt
    39901 ttagaaacat tataatttcg ttgattaatt atagggttat tagaaatgaa gggattttta
    39961 gcatattgac ctgacggcag cagtccgcca ggtcagcgtc gtaatattat gccgtccggg
    40021 caacggcatc acgcgaagcg gcatcgcgct cttcgcccgt cagctcgcta agttgcccat
    40081 tgcgcatttc cagcaggcgg tcggcgtgga taaagtaatg atcatcatga ctgatagcga
    40141 aaatagtttt acccatctcc tgcatcagcg gcagcaacac ctgataaaac tcacgacgga
    40201 agtgtggatc ctgatccgcc gcccattcat ccagcaggat aatatcgcgt tcttctgcca
    40261 gcgccagcaa cagcggcacg cgttttttct gcccttttga taacttcagg ttaacaatac
    40321 gcccgttgct taactcaagc ttatgagcca ttttcagctg cgccagccac ttctcaacca
    40381 gttgcgggtt agcgggttgc ccctccggcc ccagcagttg atcaaacagc cagacatcgg
    40441 taaacactgc cgaaaacagt ttgcgataat cttccggttg ttcgccgctg acaggtttgc
    40501 catccagcaa gatttcgccg ctttgtggct gatacaagcc cgtcaacaac atcgccagcg
    40561 tcgattttcc gctaccgttg ccgccaatca gaaacagcag ctcgccacgt ttgatggtga
    40621 gattaatcgg accaacggaa aacgcgttat cctgataagc aaacgtcacg ttacgcagct
    40681 ccagcgtttg ccagttggga aacgcctgcg ggcgcggaaa ctcagctttg aaaggcgcga
    40741 gcgcgaattt gttcagcttg ttaaacgcca cctgcgccgt cagcagcgtc ggcaatgcgc
    40801 caaccgccga aagcagcggc gtacgcagga ataacagcgt caacgaatag gtcgcagcaa
    40861 cgttggtatc agcccaaccg agactgttcg ccatccagaa caccaggccg atagcgccca
    40921 gcatcatgat gtttgaccag ttcacggcac taagatggaa ggtgtctgcg cgaataatat
    40981 gatggcgata ctcttgcgca tcggggatgt agaggttgtt aaacacatac tcggcgcgtt
    41041 cccggttcag agtcagctct ttgcgccctt caagtacagt ttgaaaatcc gtgtacagct
    41101 tgtcttcggt ttcacgtagg gtcgccatat gtttgtacac ccgcgccacc agcacaaaac
    41161 cgccccagat ggtgatcgcc atccagatag ccgttaccag caacattttg cccgacagca
    41221 tccacagata cgccgatgaa ccgatagtga gaatgatccc ctgccccagt tccggcagac
    41281 gcacaaaagc aatggtgata ttgcgcacat cgctggttaa tcccgccagc aacgaggcgc
    41341 taccgagttg ttcaatgcgc tcgacgtggg tatccagaat ccgcttgata aattcactac
    41401 gcagtcggta aacgaagtga tgccccaaag tggtgagcgc cagttgcgat ccgagagtga
    41461 ctgccatcaa cagcagcaat aatcccagaa actccggtaa caccagcaga ctggtatccg
    41521 ccgtttcgat aaggcgctga ttgataaaag caattaagcc aatgcctaat gccgcactgg
    41581 cgaggcttag cgccatcaca ctgataaatg gccagcgata ctgccgccag acaagtacaa
    41641 gaagttccat gcagaaaacc cggacaatga attacagccc gcagtttaaa catcttcgcg
    41701 cgcacagcaa taataattct tatttttatt cttttttacc tgcctgacgg aatgtcaggt
    41761 tgtagcggca gtcggtggtg agtggatgaa accccgcttt caacggttga ataccgtgat
    41821 aaaacagccg cgattcaccg ccccaggcca ccaaattgaa aaatcgcggg taagcccaga
    41881 gaaacagaaa caattggcgc gcgcagatcc ggttcgtctt tatcctgatg cagcgacagt
    41941 ttcgcgctag gagcgtagcg gttgataaga caagcatctg gctggaaatc tggatagccc
    42001 gccgccgtag ccgcacgttg acataaatca tgaaagcttt gtggcatggc gggccacggt
    42061 ttatttgttt gcggatcaat gggcgaatag agataacctt gccgatgggt cgtccagccc
    42121 agatgcccac agttggtcat cgccaccgac atggtatatc cgcctggagc gaccatctgg
    42181 cgaaacggcg actggctggc aacgtcatta atatcgcgga ttagttgctc cgcagcatta
    42241 aaagcaaaac gccgtaaaat taccgcacca gccgccagtg gctcttgcca cggttcagca
    42301 tcggcaaaca gatccaacat tacctctcct cattttcagc ttcgcggcgc agcagttgcg
    42361 ctttacgcga cacgccccag cggtaaccgg aaagtgtgcc atcaccacgg accactcgat
    42421 gacagggtat tacgatagcc agcttgttgg cggcacaggc gctggcaacg gcccgtaccg
    42481 ctttcggttt gccgatggcg ttagccagtt gctgataact gacggtttca ccacaaggta
    42541 tcgtgcgcag tgcctgccag acttgttgct gaaaagcagt gccgcgaata tccagcggta
    42601 acgtcagcgg cgtatcgcgt tgattgaggc tggcgatcac ttcacgcaca tgttgctgaa
    42661 acgtcagatc ggcaggcgcg ttgtcggcag cgggaaacat ctgctgcaac tcgctgatta
    42721 atgtcgcgtc atcatcgccc agcaatatcg cgcaaatccc ccgctcgctt tctgccacca
    42781 ggcaacgacc cagctcacaa tcagccagcg cgtaacgcac cgccagattt tcgccaccgt
    42841 gacggaattg tttagccgtc atgcccagcg tttcatcggc tttgcggtag taactgctgc
    42901 tgtcggggaa tccggcgtta agaatagacg tcgtcacgct ctcccctttc gccagcgatt
    42961 cgcgcaaacg gcgagcgcgc caggcctgtt gccaggcttt aggcgtcatt ccggtagtag
    43021 ctttaaacaa ccgatgtaga tgaaatggac tcatcgccac ctggtcggct aaggcttcca
    43081 gcgttacagg cgtttcctgt tccagcagtc gacacgcgtg ggtgatttta tccaaccgat
    43141 gttgccgggg attggctttg tctggctgac aacgtttgca ggggcgaaat ccggcggcga
    43201 gtgcctcgct ggcatttgcg tagaaggaga cattttcccg caaggcatgt ctggcgcggc
    43261 aagacggacg gcaaaagata cccgtggtgc gcacggcgaa aacgaattcg ccgtcggcat
    43321 ccgggtcgcg ggctaagaca gattgccagc gttgatcgtc agttaagcat gtggcgtttt
    43381 tcataatcag ctccctggtt aaggatagcc tttagtctgc ccggtcacca tcacgcaaaa
    43441 accaacaatc ttgcgcttta atttttttcg ctgacaagga agcttttaaa ctgtggtgac
    43501 atccaggttt taaagctatc gccttctttg gtgatcatat aaaccgccag cccttcccgg
    43561 cgaacaacct ctttggcttt ctccggcccg agtaccatca agccagtatc ccaggcatcg
    43621 gcttccagcg ccgtcggagc aatcaccgtc acggatacca gattgtgttc gatgggacgc
    43681 ccggtttgcg gatcgataac atgggaaaga cgtttgccgt ccagttcgta atagttacgg
    43741 tagctgccag aagtgctaat cccatgaccg ttgatatcca ccacagcctg aaccgcgttt
    43801 tctttatcgg ttggtttttg aatcgctacc cgccacggct ggccttcacc gttcatacca
    43861 cggctgttca gcgcgccgcc caccgacacc agatagcggg aaatcccttc ctgctccatc
    43921 aagcgtgcca ggtgatccgc cgcataacct tcaccgacgg tggagagatc gacatataaa
    43981 tccggcaggt ctttttgcag atattgctga tgcgactgat tgatgaccgt caggtgctgt
    44041 aagccggttt tggctttcat cgcatcgatc tgttcctggc tcggaatttg aaccggctgt
    44101 tgttccgggc caaagcccca cagattcacc agcggcccga cggtaatatc catcgcacca
    44161 tcggtccttg ccccaatgcg cagcgaggtg gtgacgatat cggccatcgc ttcacttacc
    44221 ggccacggcg acaaactttg cgagtcgtta aagcgcatca gcgcggaatc ttttttatag
    44281 gtcgaaagca gctgatcgtc ggcgtccagc tgggtctgaa tcttttcttt aagttcggca
    44341 ctgcgtttgg catcaatgcc cgggatgctg gcacgccaga aggtacccat ggttttgcct
    44401 tcaagaacgg taacttcagt agcgtgggtt ttggcgggtt gtggtttttg atcgcaacca
    44461 acaaagaaga gcgcggcaac cagcagtgcc acgcgggtaa agcttatttc catacgtgat
    44521 tatcctcatg cgaacggtcg caagagtaca ccaaaaaact gtgtttgtac gctgaaaaca
    44581 atgaaaaaag ggcccgcagg ccctttgttc gatatccatc gagattagaa ctggtaaacc
    44641 agacccagag ctacgatgtt atcagtgttg atgccagcgt cacgagtgaa ctggttgtcg
    44701 tccagcaggt tgattttgta gtcaacgtag gtggacatgt ttttgttgaa gtagtaggtc
    44761 gcaccaacat caacatattt caggatatct tcgtcgtcgt agttacgacc attgatgaca
    44821 cccaggtttt tacctttaga ctgcaggtaa gctagggacg gacgcagacc gaagtcgaac
    44881 tggtactgag caacagcttc gaagttctgt gctttgttcg cccaacccaa gttgcctacg
    44941 cgagttgcgt tgtaggtctg ggtgtactga gcagctaggt agatgttgtt cgcgtcgtat
    45001 ttcagaccac cggtgtaggt ttcagcgcgg tcgccattac cgatgtaacg ctcatcataa
    45061 cggttcagac cgaagttctg gtcatcagta cgtttggagc tggaaactgc agcaccgata
    45121 ccgaagcctt cgtaatcata agtaatagaa ccgccaacgc cgtcaccgtt ctgacgcaga
    45181 gcttcacgac cgttgttggt catgccttca ccttccgggc taccgttttt gccctgatac
    45241 tgaacagcga agttcagacc gtcaaccaga ccaaagaagt cggtgctacg gtaggttgcg
    45301 aagccgttac cacgctgctg catgaagttg tcagaaccgt aagtgtcacc accgaattct
    45361 ggcagtacgt cggtccagga agttacgtcg taaactacgc cgtagttacg accgtagtcg
    45421 aaagaaccta catcctggaa tttcagacct gcgaatgcca cacgggtcca ggagttgttt
    45481 tcgttttcag cgctgttgcc ctggatctga tattcccact ggccgtaacc ggtcagctgg
    45541 tcagtaacct gagtttcacc tttgaagcca agacgcatgt aggtctggtc gccatctaca
    45601 gacttgtcgt cagagaaata gtgcaggccg tctactttac cgtacagatc taatttgttg
    45661 ccgtctttgt tgtaaacttc agcagcgttt gctgctcctg ctaccagcag agctgggacc
    45721 aggagggaca gtactttaac tttcatgtta ttaaccctct gttatatgcc tttatttgct
    45781 tttttatgcc actgcatact gattaaccct cattaatcag tcggcaagtc cattctcccc
    45841 aaaaatgcag aataatccaa cacgaatatg atactaaaac ttttaagatg tttcatttat
    45901 cgctacagat gtttcaaaat gtaaatacaa gggaactttt taagattatt gcggaatggc
    45961 gaaataagca cctaacatca agcaataata attcaaggtt aaaatcaata acttattctt
    46021 aagtatttga cagcactgaa tgtcaaaaca aaaccttcac tcgcaactag aataactccc
    46081 gctatcatca ttaactttat ttattaccgt cattcatttc tgaatgtctg tttaccccta
    46141 tttcaaccgg atgcctcgca ttcggttttt ttacccttct ttacacactt ttcattattc
    46201 tgtgctacca cagaaaaact ataacgcttg ttaactattt cacaaataat taacatcagc
    46261 ataatttcca gcaatctttg tttatttaca attaatttcg ttgggctttt tgtaggttat
    46321 ttgtacagca aaatggcgct tgtacatcta tttcccccca tacaggatga taaatatcac
    46381 gggagaatag agaatcatca atcaggtaag agtctggaat ttcacactgt accctttata
    46441 ctgccctatc acttcgcgaa gttttaacag gtcataaaca cgaatgcgtc agaaagagac
    46501 aacggccacg acccgctttt cactcctacc ggggagcatt acccgcttct ttttactgtt
    46561 gatcattgtg ttactggtga cgatgggggt aatggtacaa agtgccgtta acgcctggct
    46621 gaaagataaa agttaccaga ttgtcgacat tacccacgca atccaaaagc gcgtcgatac
    46681 ctggcgttac gtgaccaggc agatctacga caacattgcc gcgacgacct ccccctcctc
    46741 cggcgaaggt ttacaagaga cgcgcctgaa acaggatgtc tactatctgg agaaaccacg
    46801 ccgcaaaacg gaagcgttaa tctttggctc tcacgacaac tcaacgcttg agatgactca
    46861 gcggatgtcc acttatctgg atacattgtg gggtgcagaa aatgtaccgt ggtcgatgta
    46921 ttacctgaat ggtcaggata acagtctggt gctgatctca accctacccc ttaaagatct
    46981 cacctccgga tttaaagaat cgaccgtcag tgacattgtt gattcacgtc gtgcagaaat
    47041 gttgcaacag gccaacgccc tcgatgaacg cgaaagtttt tctaacatgc gccgcctggc
    47101 ctggcagaac ggtcattact ttaccttgcg taccacgttc aaccagccgg gacatctggc
    47161 aacggtcgtg gcttttgatc tgccgattaa tgatttgatc ccaccgggta tgccgctgga
    47221 cagtttccgc cttgagccag acgcgacggc aacgggaaac aatgataatg agaaagaagg
    47281 gacggatagc gtcagtatcc actttaacag tacgaagatt gaaatctcct cggcactcaa
    47341 ctctaccgat atgcgcctgg tctggcaggt tccttatggc accttattgc tggatacgtt
    47401 gcaaaacatt ctgctgccac tgctgctgaa catcggtttg ctggcgctgg cgttatttgg
    47461 ctataccaca ttccgccatt tctccagccg cagtacagaa agcgtcccca gcacggcggt
    47521 caataacgaa ttgcgcattt tacgggcaat taatgaagag atagtctcac tgttgccgct
    47581 cggcctgctg gttcacgatc aggaatcgaa ccgcactgtc ataagtaaca aaattgccga
    47641 tcatttgctg ccgcatctga atctgcaaaa catcaccacc atggcggaac agcatcaggg
    47701 gattattcag gcgacgatca ataacgagct gtatgagatc cgcatgttcc gcagccaggt
    47761 cgcgccgcgc acacaaattc tcattattcg cgatcaggat cgcgaagtgc tggtaaacaa
    47821 gaaactcaag caggcgcagc gtctgtatga gaaaaaccag caggggcgga tgacctttat
    47881 gaaaaacatt ggcgatgcgc tgaaagaacc cgcacagtcc ctggcggaga gtgcggctaa
    47941 actcaacgcc ccggaaagca aacaactggc gaatcaggca gatgtgctgg tgcggctggt
    48001 cgaagaaata cagttagcga acatgcttgc ggatgatagc tggaaaagtg agacggtgct
    48061 gttctccgtg caggatttaa ttgatgaagt tgtgccttca gtgttgcctg ccatcaagcg
    48121 taaaggtctg caactgctga ttaacaatca tctgaaagca cacgatatgc gccgcggcga
    48181 tcgcgatgcc ttacgacgta ttttgctgct actgatgcaa tatgccgtga cctcaacgca
    48241 attgggaaaa atcacccttg aggttgatca ggatgagtcc tctgaagacc gcctgacatt
    48301 ccgcattctg gacacgggag aaggcgtaag tattcatgaa atggataatt tgcacttccc
    48361 gtttatcaac cagacccaaa acgatcgtta tggaaaggcg gacccgctgg cattctggct
    48421 gagcgatcaa ctggcacgta aactgggcgg tcatttaaac atcaaaacgc gggatgggct
    48481 tggtacacgc tactctgtgc atatcaaaat gctcgccgct gacccggaag ttgaagagga
    48541 agaagagcgt ttactggatg atgtctgcgt aatggtggat gttacttcgg cagaaattcg
    48601 gaatattgtc actcgcctgt tagaaaattg gggtgcaacc tgtatcacac ccgatgaaag
    48661 attaattagt caagattatg atatcttttt aacggataat ccgtctaatc ttactgcctc
    48721 tggcttgctt ttaagcgatg atgagtctgg cgtacgagaa attgggcctg gtcaattgtg
    48781 cgtcaacttc aatatgagca acgctatgca ggaagcggtc ttacaattaa ttgaagtgca
    48841 actggcgcag gaagaggtga cagaatcgcc tctgggcgga gatgaaaatg cgcaactcca
    48901 tgccagcggc tattatgcgc tctttgtaga cacagtaccg gatgatgtta agaggctgta
    48961 tactgaagca gcaaccagtg actttgctgc gttagcccaa acggctcatc gtcttaaagg
    49021 cgtatttgcc atgctaaatc tggtacccgg caagcagtta tgtgaaacgc tggaacatct
    49081 gattcgtgag aaggatgttc caagaataga aaaatacatc agcgacattg acagttatgt
    49141 caagagcttg ctgtagcaag gtagcctatt acatgaacaa tatgaacgta attattgccg
    49201 atgaccatcc gatagtcttg ttcggtattc gcaaatcact tgagcaaatt gagtgggtga
    49261 atgttgtcgg cgaatttgaa gactctacag cactgatcaa caacctgccg aaactggatg
    49321 cgcatgtgtt gattaccgat ctctccatgc ctggcgataa gtacggcgat ggcattacct
    49381 taatcaagta catcaagcgc catttcccaa gcctgtcgat cattgttctg actatgaaca
    49441 acaacccggc gattcttagt gcggtattgg atctggatat cgaagggatc gtgctgaaac
    49501 aaggtgcacc gaccgatctg ccgaaagctc tcgccgcgct gcagaaaggg aagaaattta
    49561 ccccggaaag cgtttctcgc ctgttggaaa aaatcagtgc tggtggttac ggtgacaagc
    49621 gtctctcgcc aaaagagagt gaagttctgc gcctgtttgc ggaaggcctc ctagtgaccg
    49681 agatcgctaa aaagctgaac cgcagtatta aaaccatcag tagccagaag aaatctgcga
    49741 tgatgaagct gggcgtcgag aacgatatcg ccctgctgaa ttatctctct tctgtgacct
    49801 taagtccggc agataaagac taatcatctg taggccagat aagacgcgtt agtgtcttat
    49861 ctggcatttg caccgattgc cggatgcggc gtaaacgcct tatccggcct acgattccca
    49921 ttatttcaac agattacatt aaagtaggcc agataagacg cgtcagtgtc ttatctggca
    49981 tttgcactga atgccggatg cggcgtaaac gccttatccg tcctacgaat cccgcgattt
    50041 cctgaccctc tcggcatata ccgtcagcgt cttttttatc acatccagcg ttaccggctt
    50101 cgagaggcag ctgtccatac cggactccag acaccgctgc ttctcttcag ccaacgcatt
    50161 agcagttact ccgattaccg gcaacgtcag tcccaactga cgaatgcgtt gcgtcaagcg
    50221 gtaaccatcc atatttggca tgttgacgtc gctaagcacg atatcaatat gattcttact
    50281 aagtacatta agcgcatcga cgccatcatt cgcggtttta cattgatagc ccaacgatcc
    50341 caactgatct gccagcagac gtcggttaat cggatgatca tccacgacca gaatcatcat
    50401 atcgtcgtta tcgctgaccg ctttgtccgt cgacggcaga gcgttagcag gatcgtcgct
    50461 ctccatctcg atcaaataaa tacgcgccaa caatgccggt agctcatgcg gagcagccac
    50521 actgtgtacc cactcccctg gcgctttctc cagcggaata ccaatatggc gacgacagaa
    50581 ggtcactacc gctctgccct gccatttttt actcactacc tcgtcagtga ttaacacatc
    50641 ttcgggagtc ggttcctgcc cttcgtatgt tgtaacgacg atgccgctgc gctgcaaact
    50701 ggtttccagg aactgacaga gcgacgcatt gcggaccgcc agccagcagc gtttaccact
    50761 caacccttcc acgccttttt tctgcgggta ctgagcgccg tacaacggaa tacgcacggt
    50821 aaactggctg cccattcccg gttctgaatc taccgagata tcgccgtcca tcatgctgat
    50881 cagtttttca caaatcgcca gacccagacc ggtcccctgg aagtttcgct gtacgcccgt
    50941 tccaacctgg aagaagggat caaacaagcg caccacttct ttcgccggaa tccccacgcc
    51001 ggtatcgcga acacggatag agagataatc gccatcagcg cgaacatgca aaactataca
    51061 gccggtatcg gtgaatttta tggcgttact caacaggttg gagatgacct gctgtaaacg
    51121 catcgggtcg ccatttaagg ccactggcac atccggttca ataaagcagt acaagcctaa
    51181 ctgcttgcgt accaccagcg gtaaatagtt ggcggtgatg tggttcatca cttcacgcgg
    51241 tgaaaactca cgcggttcga tcttcaactg ttccgattca atcttcgaga aatcgagaat
    51301 atcgctgata attttcaaca acaggctgga agagttgttc attgccgtca ccagacgatc
    51361 gacgcctttc ggtaactctt tggtttgcaa cagatccagg ttaccgataa tgccatacag
    51421 cggcgttcgc agctcatgac tgacggtggc aaggaacatc gattttgact ggctcgcctg
    51481 ttccgctgct tgtgccatct cctgcaacga ctcttccatc ttcacgcgcg aagaaacatc
    51541 caccagcaca caaatggcca cgttttcatt acgatagcgc gaatggacga agctgatttg
    51601 cagattggta ttgttgctgg tcaggacatc aacaaaattg acctgctgcc cacagataat
    51661 ttgcgtcagt cgttggcggt cctcatgcgt aagcatattg agataggtat gcgccagttc
    51721 gttacttaaa atattgacgc catcagcggt acgcaaaatg cagataccca ctggcgcgga
    51781 ggcgacaatc ttgcgattga actgctcatg ttcttccagt cgcagggcgt cgctttccgc
    51841 cggaatgaaa atacgtcgct cgtacatccg cgcgagagta aacaatgcag ctccggcaag
    51901 cacattcagc aaaattgcgt taaggatcaa catgcgaatg cgttccagca ccttatcaac
    51961 cggcaccgaa tacacgatgc ttagcgatga gggtggcaga tttttcttca gcaccagctc
    52021 ccggaaccct tccgtatagc caaaccagga gcgttcctgc atccagcgag gatcgccctt
    52081 aattttactt tctggtccgg taagcgaaat cagggtatga ccattttcat caagaatggt
    52141 aacccccatc ggcaacgtac ccggtaagaa aaagttctcc atccggatgg tctgctcgac
    52201 acccaaaagc gcctgcaacc ggttcgccag ataaactggc gtcaacgcgt aaaaataccc
    52261 gacgccaggg cgcggacctt cgctgatcca gtagaggtta ctgccgctat catcttgtgg
    52321 tgcatttcga tatttattga tgcgttcatg caacgctttc aacgcggtat cgcgttccac
    52381 tggcatatca cgcagaccga aattggccat gcagaggtta tcactgccga ttaaaaatac
    52441 ccggttgaga tcgtaagccg cagaaaaatt atcgcgccag tagcgcataa accacgccaa
    52501 tgactccaga gaacctcgcc aggtgttact cattgcggaa caatcggagt cggcaaacag
    52561 cggttcaaac gcaggcacat ccgcctgcgt ttctcgtcca cgcggggaaa gcacaccgtt
    52621 ttctgccgat aagcgatttt cggcgatgta cttaagctct ttcatcacat cagaggtgcg
    52681 ttgaataaag cgctgagcct gatcggaact cagattaaat tcctgacgaa tttccgattc
    52741 tcgctgatgt aacgcattaa cgatgtaaaa aacggatgaa aaagcaatca acagccagag
    52801 cactaacgcc aatgctctga acatgtagcg cgaggctttc agggttgtac gaaaagaagc
    52861 aaggtatttc aaaggggcga agctccgcct caggtgaccg atggagtgtg gttaaggtag
    52921 cggtaaaagc gtgttaccgc aatgttctgt caccattgca ataatgcgta tcgcttagaa
    52981 accattgcct taaccaggaa agccggagta gatggacagt tctcagtacg aaaaataaga
    53041 aatgaccaat ctgccgacgc ttgttaatgg ttatatcctg gcgtcggcag atcgcccagc
    53101 agtcttatct gaaaaaagag agagccaggt tgtgtgaagg gatggatcac cagtcaaaac
    53161 ttttcaccag cgtcagctcg ccagcattac gcatcggtac aataaatgtt tcctgtttct
    53221 cattgaccga tccttcatcg gtgatcagcg tcagttgggc ggtcgtcaat tccgtttcgc
    53281 tgcgcccacc atagtagttg atatacacct gatagcgccc gtgaactggt gctggcatgg
    53341 cgaaaatctc gggtccgtac cccgtcgtga catccatatc cagtgcacca ctgtttttca
    53401 gcacggtgtt accgtaccag gcgtgttcgc catcgggcgt gacaacatgg agatcgagat
    53461 cggtattgtc catatcccac gagagaacca gccgtaaacg tgcacgaata gttcctgtac
    53521 ccggcgttga gtaaaactgc attttttgcc ggctttgccc atccgggctg atgacctgca
    53581 cgctgttgct gccttcagtg aaaatataag ggcgtgcaaa tgaaccatcg gattcaattc
    53641 tctgcggcat actggcaccg ttgaccacca gccggccctg ctgaacttta cccgccgcag
    53701 cataattctt tattttgccg ctgatctgcg ctgagatatt ttgatcatct gccatgttga
    53761 cggacgacgc cgggtaattg atcgattgtg aaaaactggc atcttcgccc tctgccggat
    53821 gccagccaga gagcggtgcg tcaatctcga cttcctgcac accttcactg tgagcgacag
    53881 gcgaaagtgc caccagtaac aacggaagaa aaatctttcg catagcatta gtccattaat
    53941 aattgtcggg tcagcgtttc gatatagttc tcatcaagcc cggtgggata accatcaaag
    54001 gccaagtgca gatactcatg ggttaaatcc agccgatcct gaagcgtaaa gaagttacga
    54061 ataaacagcc gtttctgctg ccgatcggta taggggaaac ctgagactaa acgacagacc
    54121 gcaaacacgt ctggttcgtt gtaccccgtc tcgccttgta atatacgccg ccactgcggc
    54181 atttttttcg ccagccaggc tttcgctttg ggtaataact ggcaggttga gcgtggagct
    54241 ccccagcggc taaggctgtt gtcgggataa gcaaacgcga gtatctggtc gtaacgctcc
    54301 ccctgccccg cctgggccgt ggcttgccgc caggaaagtg tcccttcggt agcgcgactg
    54361 ccatgataat gaaccggatc gcctgcatag atgagctcct gcgtccaggc ggtcatcgtt
    54421 cgcgcccccg tggtcgctgg cgaagcagaa acacgctgcg tggcgctgct gccggggatg
    54481 gtcaggcaat caccttcccg attggcgttt tgctgtaaaa aagtacgtat cgccacagtc
    54541 atggctttcg ctgcctcagg tggcgtactt ttcgcctcgc gatccagaac tctggcaacg
    54601 tactcttcac gatcgagatg cgactgcaat ttcagtttgc ccttttcgct cagtaaggtc
    54661 gtctcaccgt ggctgacaaa cgtaatgtgg ttaccattag taaacgtcac acgatagcgc
    54721 ccattaagta cgctgggttt tactgccgtc gtactctttt ccgctgtgat tttcttcagc
    54781 ggatagcggg caaacagctc cacctctaca cattgcccac tggcaacctg cgtcggcact
    54841 ggcaaaaccc gattaagcac cgtcgcataa cgggttagta ccgttttgct ggtgccactg
    54901 ccagtaaccc acagcggtgt accatcggtt aaccagccag caaaaccacc ctgacggatt
    54961 tcctgtttat catcggcaaa ccagctccag gttttcaccc gtacccgact accgagcatg
    55021 gatgagacac cgattttcgc ctcgtccagc accacatcca gtaagacttc ctgagctttg
    55081 ttttgcgcag gtaacgtcgc taatgaatca agcagcgatt taaccgttac cgaggtttct
    55141 ggcttcagcg ttgtaagaga cgctaaccac gcaggtgcct gacgctgttg ccagtattgc
    55201 ccccacacat ctgcgccaat atgcaaccgc tgcggcgcaa aatagagccc acaggaacgt
    55261 accagcgcgg tatcgcgcgt tatgctctcc ccagcctgac aacaataaac ctcttccggg
    55321 gagtttccgc gacactggta gggctgctcc ggttgatggg tatcctccag ccaggcgtaa
    55381 acatacagtt tccacaaact ccctaacggc gtggtgagcg agtcaggtaa tgctgagact
    55441 ttggtcacgc ctgatgacga cagctgatac agttgatcgt gctgcgcacc gcgcagcacc
    55501 agttgtaacg gcgtctcctc cgcaagcgtt ggcagagtaa ccaacgccaa tagccagacg
    55561 attctgcgcc agttcactta tttcacctgc atcccggtcc attcactccc tgccgcgaca
    55621 ctttgctgcg caggtgcata ggaacgcaca taacgagcag gaggcagaac gaattgccct
    55681 ttttgcgaga agcgcagcaa atggcggaaa gtgaccgttc ccgtcagttc tttcaccggc
    55741 accatatagg ccagttcgcc catttcatta cgcgcttttt ccagcaattg cccctgctgt
    55801 ttcgcggcgt tgggtttatt gactgaaatg ccccatgttg tgcgctcaac gtcggctccc
    55861 ggcgggagcg gtacttccac ctgaccatag cgcagaactg catcctgctc gcaggtaagc
    55921 gtaatttcat cgagatacag cgcatcgctg tcaatctcat tgctggtcac cggctgcaga
    55981 gtaaagctca tctcttcttc accggggata agccgataca actggcgttc aacggtcacc
    56041 ggaatgttac tttgttgagc cgtttttgcc ggttcacgcc agcggacctg cacattttgc
    56101 ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctgaccaac ccaacgccag
    56161 tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc aggcaaaaca
    56221 actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc gcgctcaata
    56281 gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg tcaccaccgc
    56341 tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct ttatcccacg
    56401 catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc agcaacgtca
    56461 tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg aggatcaggc
    56521 tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt ccgcgccggg
    56581 cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc tcctgtgccc
    56641 acgccagcac cagcgcccga tgcaataacg gcatgttatc cgcctgctcg gcataactgt
    56701 cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg ctggcctgcc
    56761 aatcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct tcaccccacc
    56821 aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta tcctgaatca
    56881 tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc caggcgagac
    56941 tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca tcgactaacg
    57001 catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc cggatattac
    57061 tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca tttttctgct
    57121 gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg tcctgcactt
    57181 gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc tgcgtcagcg
    57241 aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg cctgcaaatt
    57301 tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt cctgccgccg
    57361 gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga tttttttccg
    57421 aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca cgcgcggtga
    57481 tacgccagcg ggttaac
//
LOCUS       DAJTYI010000009        56951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYI010000009 DAJTYI010000000
VERSION     DAJTYI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            98..574
                     /locus_tag="OO564_000568"
     CDS             98..574
                     /locus_tag="OO564_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6651818.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            926..2038
                     /gene="potF"
                     /locus_tag="OO564_000569"
     CDS             926..2038
                     /gene="potF"
                     /locus_tag="OO564_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6651819.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            2133..3209
                     /gene="potG"
                     /locus_tag="OO564_000570"
     CDS             2133..3209
                     /gene="potG"
                     /locus_tag="OO564_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6651820.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            3276..4229
                     /gene="potH"
                     /locus_tag="OO564_000571"
     CDS             3276..4229
                     /gene="potH"
                     /locus_tag="OO564_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6651821.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            4226..5071
                     /gene="potI"
                     /locus_tag="OO564_000572"
     CDS             4226..5071
                     /gene="potI"
                     /locus_tag="OO564_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6651822.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            5131..5619
                     /locus_tag="OO564_000573"
     CDS             5131..5619
                     /locus_tag="OO564_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6651823.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            5660..6787
                     /gene="rlmC"
                     /locus_tag="OO564_000574"
     CDS             5660..6787
                     /gene="rlmC"
                     /locus_tag="OO564_000574"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6651824.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(6962..7693)
                     /gene="artJ"
                     /locus_tag="OO564_000575"
     CDS             complement(6962..7693)
                     /gene="artJ"
                     /locus_tag="OO564_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6651825.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7985..8653)
                     /gene="artM"
                     /locus_tag="OO564_000576"
     CDS             complement(7985..8653)
                     /gene="artM"
                     /locus_tag="OO564_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6651826.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(8653..9369)
                     /gene="artQ"
                     /locus_tag="OO564_000577"
     CDS             complement(8653..9369)
                     /gene="artQ"
                     /locus_tag="OO564_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6651827.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(9376..10107)
                     /gene="artJ"
                     /locus_tag="OO564_000578"
     CDS             complement(9376..10107)
                     /gene="artJ"
                     /locus_tag="OO564_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6651828.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(10125..10853)
                     /gene="artP"
                     /locus_tag="OO564_000579"
     CDS             complement(10125..10853)
                     /gene="artP"
                     /locus_tag="OO564_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6651829.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(11071..11586)
                     /locus_tag="OO564_000580"
     CDS             complement(11071..11586)
                     /locus_tag="OO564_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6651830.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            12235..14004
                     /locus_tag="OO564_000581"
     CDS             12235..14004
                     /locus_tag="OO564_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6651831.1"
                     /translation="MINNISDQASSFPGTQLNQSDHFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            14215..14538
                     /locus_tag="OO564_000582"
     CDS             14215..14538
                     /locus_tag="OO564_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6651832.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            14535..15365
                     /gene="amiD"
                     /locus_tag="OO564_000583"
     CDS             14535..15365
                     /gene="amiD"
                     /locus_tag="OO564_000583"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6651833.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(15362..16375)
                     /locus_tag="OO564_000584"
     CDS             complement(15362..16375)
                     /locus_tag="OO564_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6651834.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(16474..17904)
                     /locus_tag="OO564_000585"
     CDS             complement(16474..17904)
                     /locus_tag="OO564_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6651835.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(17915..18916)
                     /gene="ltaE"
                     /locus_tag="OO564_000586"
     CDS             complement(17915..18916)
                     /gene="ltaE"
                     /locus_tag="OO564_000586"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6651836.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(18953..20671)
                     /gene="poxB"
                     /locus_tag="OO564_000587"
     CDS             complement(18953..20671)
                     /gene="poxB"
                     /locus_tag="OO564_000587"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6651837.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(20804..21772)
                     /gene="hcr"
                     /locus_tag="OO564_000588"
     CDS             complement(20804..21772)
                     /gene="hcr"
                     /locus_tag="OO564_000588"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6651838.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(21784..23436)
                     /gene="hcp"
                     /locus_tag="OO564_000589"
                     /gene_synonym="priS"
     CDS             complement(21784..23436)
                     /gene="hcp"
                     /locus_tag="OO564_000589"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6651839.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(23580..24479)
                     /gene="lysO"
                     /locus_tag="OO564_000590"
     CDS             complement(23580..24479)
                     /gene="lysO"
                     /locus_tag="OO564_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6651840.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(24936..25631)
                     /gene="aqpZ"
                     /locus_tag="OO564_000591"
     CDS             complement(24936..25631)
                     /gene="aqpZ"
                     /locus_tag="OO564_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6651841.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            26056..27714
                     /locus_tag="OO564_000592"
     CDS             26056..27714
                     /locus_tag="OO564_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6651842.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(27711..28667)
                     /locus_tag="OO564_000593"
     CDS             complement(27711..28667)
                     /locus_tag="OO564_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6651843.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            28818..29933
                     /gene="macA"
                     /locus_tag="OO564_000594"
     CDS             28818..29933
                     /gene="macA"
                     /locus_tag="OO564_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6651844.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            29930..31876
                     /gene="macB"
                     /locus_tag="OO564_000595"
     CDS             29930..31876
                     /gene="macB"
                     /locus_tag="OO564_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6651845.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(31949..32173)
                     /gene="cspD"
                     /locus_tag="OO564_000596"
     CDS             complement(31949..32173)
                     /gene="cspD"
                     /locus_tag="OO564_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6651846.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            32496..32816
                     /gene="clpS"
                     /locus_tag="OO564_000597"
     CDS             32496..32816
                     /gene="clpS"
                     /locus_tag="OO564_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6651847.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            32847..35123
                     /gene="clpA"
                     /locus_tag="OO564_000598"
     CDS             32847..35123
                     /gene="clpA"
                     /locus_tag="OO564_000598"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6651848.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(35526..35613)
                     /locus_tag="OO564_000599"
     tRNA            complement(35526..35613)
                     /locus_tag="OO564_000599"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35577..35579),aa:Ser,seq:gga)
     gene            complement(35867..36085)
                     /gene="infA"
                     /locus_tag="OO564_000600"
     CDS             complement(35867..36085)
                     /gene="infA"
                     /locus_tag="OO564_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6651849.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(36370..37074)
                     /gene="aat"
                     /locus_tag="OO564_000601"
     CDS             complement(36370..37074)
                     /gene="aat"
                     /locus_tag="OO564_000601"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6651850.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(37116..38837)
                     /gene="cydC"
                     /locus_tag="OO564_000602"
     CDS             complement(37116..38837)
                     /gene="cydC"
                     /locus_tag="OO564_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6651851.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(38838..40604)
                     /gene="cydD"
                     /locus_tag="OO564_000603"
     CDS             complement(38838..40604)
                     /gene="cydD"
                     /locus_tag="OO564_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6651852.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(40727..41692)
                     /gene="trxB"
                     /locus_tag="OO564_000604"
     CDS             complement(40727..41692)
                     /gene="trxB"
                     /locus_tag="OO564_000604"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6651853.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            42237..42731
                     /gene="lrp"
                     /locus_tag="OO564_000605"
     CDS             42237..42731
                     /gene="lrp"
                     /locus_tag="OO564_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6651854.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            42866..46972
                     /gene="ftsK"
                     /locus_tag="OO564_000606"
     CDS             42866..46972
                     /gene="ftsK"
                     /locus_tag="OO564_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6651855.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            47127..47738
                     /gene="lolA"
                     /locus_tag="OO564_000607"
     CDS             47127..47738
                     /gene="lolA"
                     /locus_tag="OO564_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6651856.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            47749..49092
                     /gene="rarA"
                     /locus_tag="OO564_000608"
                     /gene_synonym="mgsA"
     CDS             47749..49092
                     /gene="rarA"
                     /locus_tag="OO564_000608"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6651857.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            49183..50475
                     /gene="serS"
                     /locus_tag="OO564_000609"
     CDS             49183..50475
                     /gene="serS"
                     /locus_tag="OO564_000609"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6651858.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            50715..53159
                     /gene="dmsA"
                     /locus_tag="OO564_000610"
                     /pseudo
     CDS             50715..53159
                     /gene="dmsA"
                     /locus_tag="OO564_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            53170..53787
                     /gene="dmsB"
                     /locus_tag="OO564_000611"
     CDS             53170..53787
                     /gene="dmsB"
                     /locus_tag="OO564_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6651859.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            53789..54652
                     /gene="dmsC"
                     /locus_tag="OO564_000612"
     CDS             53789..54652
                     /gene="dmsC"
                     /locus_tag="OO564_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6651860.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(54688..55314)
                     /locus_tag="OO564_000613"
     CDS             complement(54688..55314)
                     /locus_tag="OO564_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6651861.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            55629..56777
                     /locus_tag="OO564_000614"
     CDS             55629..56777
                     /locus_tag="OO564_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6651862.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        14075 a        14068 c        14989 g        13819 t
ORIGIN      
        1 gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt tgcgtaagct
       61 gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg
      121 tctggatacg ctgcgtcaat ggctcgatgg cctggggatg agtttttttg aatgtaataa
      181 ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat
      241 cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt
      301 attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt
      361 tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat
      421 gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc
      481 gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca
      541 aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca cgcagtcgct
      601 gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct taaagattat
      661 ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat aaaaatttgt
      721 taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga
      781 tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta caccgcgccg
      841 ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg ttcgtttgtt
      901 aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc tatcgggtct
      961 ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac aaaaaacact
     1021 ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt ttgaaaaaga
     1081 aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg aaggcaaatt
     1141 aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc ttgaacgcca
     1201 gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt ggaagaatct
     1261 cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat ttgctatgcc
     1321 ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag ctgtgctggg
     1381 cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc tggaaaaact
     1441 gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa
     1501 ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac cggcaaccga
     1561 tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat acattaacga
     1621 cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc
     1681 aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc caaaagaagg
     1741 ggcgatggcg ttctttgatg tattcgccat gcctgcggat gccaaaaaca aagacgaagc
     1801 ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt
     1861 gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag tccgtgagaa
     1921 cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga aagtgcagga
     1981 cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc
     2041 cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac catcatggtg
     2101 cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct cgcccgcagg
     2161 cgaaaacccg taaggtgctg acgccgctat tagaaatccg caacctcacc aaatcctacg
     2221 atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc
     2281 tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac
     2341 aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc
     2401 tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg accgtggaac
     2461 agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt tccagtcggg
     2521 tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc
     2581 tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac
     2641 tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc
     2701 ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc
     2761 aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg aaatttgtcc
     2821 agattggcga accggaagag atctacgagc atccgactac ccgctatagc gccgaattta
     2881 ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc
     2941 ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg gtggtcgata
     3001 acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg
     3061 ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc ggcgatcttt
     3121 cggtgtatca cgttcgtctg aaaagtgggc agatgattag cgcccagcta caaaacgccc
     3181 atcgtcatcg taaagggtta ccgacctagg gcgacgaagt gcgtttgtgc tgggaagtgg
     3241 acagcagtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa cctgctgccc
     3301 agtcgaaacc gccgggcgga tttaagctgt ggttgtcaca gctgcaaatg aagcatgggc
     3361 gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg ctgccatttc
     3421 tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct tataccgaac
     3481 tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat tttctgcaac
     3541 tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg gctgcgattt
     3601 cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg cacagtaagc
     3661 cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc tcgtttctga
     3721 tccgcgttta tgcctggatg ggaatattaa aaaacaacgg tgtgctgaat aattttctgc
     3781 tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg gcagtttata
     3841 ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc gcgttgattc
     3901 gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt
     3961 tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg atgctggtgt
     4021 ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg gacagcatca
     4081 tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg gtggcctcgg
     4141 cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc
     4201 agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt cgccctggcg
     4261 gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga tgctggttat
     4321 ctattcgttt aacagctcga agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta
     4381 tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc
     4441 tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg tgctggtgcg
     4501 ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc cgctggtaat
     4561 gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat
     4621 tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca ctttctgtac
     4681 ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga
     4741 agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat
     4801 gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc ttgatgatct
     4861 ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc tggtcttttc
     4921 cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga tcctcggcgc
     4981 ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa aacagcggat
     5041 acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa cctggctaca
     5101 taatgctgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg attttttaag
     5161 aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc
     5221 gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc
     5281 ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct ggtcttttta
     5341 agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt gaaagggcgt
     5401 cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg
     5461 gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa
     5521 atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat gctgctgttc
     5581 gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc gtcgcccctg
     5641 atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg
     5701 tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg atcttaaaaa
     5761 tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg
     5821 gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat
     5881 gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc cagcctcatt
     5941 tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa cgccctacaa
     6001 cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc agagtgatgg
     6061 cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc
     6121 gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca atattcagcc
     6181 ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac aacaggcact
     6241 ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct tccagactaa
     6301 tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac agctgccggt
     6361 taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc
     6421 tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg caaagcagtc
     6481 agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca ctcagtttgc
     6541 cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc gcggcattgg
     6601 taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct actccagctg
     6661 taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca tcgaacgggt
     6721 acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa
     6781 gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt tgttcatgcc
     6841 ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat acattgcaga
     6901 gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc atcagtctca
     6961 attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct ttaattgctg
     7021 ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca gcgataccca
     7081 ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc agttgcggat
     7141 tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca atacgaccat
     7201 ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc acttccgggt
     7261 gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg cgtttgcctt
     7321 tcaggtcggc aaacgttttg taggtatctt ttttggcaat cacgacggct gagttttcat
     7381 agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc ataccggaga
     7441 ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac gcgtgattag
     7501 taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg atatcaaagc
     7561 cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct gaaacgccaa
     7621 aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa
     7681 ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg
     7741 gcatgggata acaatgccac caagtgaatt tatatgcaat aaacatgatt aaataattta
     7801 aatgaaataa aaaaagacgg ataacttagt tagttgtccg tcttcattat aagaatttat
     7861 gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt
     7921 ctgatgcgac gctggcgcgt cttatcagac ctacaaatcc ccccggcgaa tgtacgcagc
     7981 tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc atcatcagcg
     8041 tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt
     8101 aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag
     8161 ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt
     8221 taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac
     8281 aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat
     8341 acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct gacaacaaat
     8401 gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa taaatcagga
     8461 agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca
     8521 ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca
     8581 caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc agctcgggta
     8641 aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt
     8701 gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca
     8761 ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta aatcattcac
     8821 actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc
     8881 aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga
     8941 cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca acgcgccgcg
     9001 cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa
     9061 cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga acccaagatt
     9121 gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat agataaacag
     9181 cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc
     9241 gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg ccagcccgac
     9301 aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa
     9361 ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc
     9421 tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt tgccctgacg
     9481 taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat cgcccactgc
     9541 cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac caaatacgct
     9601 gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt
     9661 ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc
     9721 gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag
     9781 ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct ccggagtgat
     9841 atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc
     9901 aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg cctgtgccag
     9961 gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggatagga
    10021 ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat
    10081 taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag
    10141 tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata
    10201 tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg
    10261 gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt
    10321 gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc
    10381 atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg
    10441 taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc
    10501 gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg
    10561 gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg
    10621 cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc
    10681 gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg
    10741 ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac
    10801 agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact
    10861 cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta
    10921 tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa
    10981 aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa atgccggata
    11041 atttacgtgg tttattggtt gatcaaggcg ttagcgattc tcgatggact gacggagcgt
    11101 acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga cccaacactg
    11161 gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt tgagatccac
    11221 ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg caacgtgggc
    11281 gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt cacgatgact
    11341 attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat aggggcgcag
    11401 ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt gagccacact
    11461 gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat aagctggcgt
    11521 gacggtggta caggcactca gcagcagtgc acatggaata agtagagata gtgaacggta
    11581 gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca aacgctatca
    11641 gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc tgattgggtt
    11701 tgagtccatt gctggctacc cactcaaggt tgctcgtagc tgacttactt aaattaccca
    11761 aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg aaatgatcat
    11821 ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga ggttaagagc
    11881 atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg ttattgtcta
    11941 atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga ttgccaaaat
    12001 tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact ctcgataata
    12061 ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt gaagaataaa
    12121 ttaaattgct tcagtatttc gttaattaat atgaatacaa aaggtgttgt gcttttataa
    12181 ttataaagcg cgaggagttc ggtggattat cgtttttctt ggatgatttt taatatgata
    12241 aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca aagtgatcat
    12301 ttcctggatt cactgaggga atttttcgcg atattaaatt ccagtcgtaa gggtgagttg
    12361 tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga cagtgatctc
    12421 ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca atttaataca
    12481 ttttttagca atgctttcga gattgatgtt aaaaaatatc tgggtaaatc agaagataac
    12541 gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa tgtatcgaat
    12601 ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa attacaagaa
    12661 caattagata agtattttac gattgaatcg cacccattat tatacaggta taccattggt
    12721 agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt taacaaagaa
    12781 caatatatat cctttataaa ggacgcattt attcattttt atgattatag caggcgatat
    12841 gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt gatgagcact
    12901 ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac agaagaagag
    12961 tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc tgatgataat
    13021 tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc ctttcaagat
    13081 accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat ttgtagcgta
    13141 agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa tgaagaagtt
    13201 ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag taatttagtc
    13261 aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt taatgacgtg
    13321 gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt gaatgaaagc
    13381 atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat aacaaatagc
    13441 aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat taataacgta
    13501 aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat gataagagtt
    13561 aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat aaaaccctgg
    13621 ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg tttactatct
    13681 ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa cgtgaaggtt
    13741 gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg tattaaacta
    13801 ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt cgcttatatt
    13861 aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga ccaaaaagca
    13921 atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca tttacaatat
    13981 aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca ggtaagcggt
    14041 accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac ttaagtgggt
    14101 gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc aacatggcat
    14161 atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc gatcatgcaa
    14221 ttttctacaa ccccaactct ggaaggccag accatcgttg aatattgcgg tgtggtgacc
    14281 ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat ccgcgatatc
    14341 gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga gatcgccttt
    14401 gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg tattgatatc
    14461 gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag cggtacggcg
    14521 gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg ttactggcag
    14581 ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat acccgacacc
    14641 aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc gcagatgatt
    14701 ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat ctgatccctg
    14761 cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg gaacaagaac
    14821 tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac gacacctcta
    14881 ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa tattttgccc
    14941 cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt attgcccgtt
    15001 atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag cgcaaagacg
    15061 atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt gcctggccgg
    15121 atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg gtagataccg
    15181 cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat atgacaccgc
    15241 gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg ttatataacg
    15301 gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa tacgggcagg
    15361 attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg atgccttcat
    15421 ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata tccagcgtaa
    15481 agtcaaaatt caacttggag acgccgtagt gggtcagcgg cggctctttt gctgacttgc
    15541 ggcctaaacg ctccatgctg tgggcgatca tatccagcat cgggtaaggg acggaacgaa
    15601 tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat
    15661 gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag gcttcctggc
    15721 ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc agcgcactgc
    15781 cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga cggggaataa
    15841 agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg
    15901 gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg cgggcaaact
    15961 cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatc gcgatggtgg tgataatcga
    16021 agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg acagcccatt
    16081 cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt gtcccccagg
    16141 gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc actttagctt
    16201 gtgatgaaac cagctcggtc agatccgtca gaacaaactc tgcgcccatt ttttccagca
    16261 atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc tggcataaaa
    16321 actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc ttcatatcaa
    16381 tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca tgatgcaatg
    16441 ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt ctgccagtct
    16501 ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca tcaataacca
    16561 gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga tagtacgata
    16621 gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt tcatgccaaa
    16681 taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat ccaccgcatc
    16741 gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac cgatcgcgcg
    16801 gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct ctttaccgcc
    16861 gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa cggtaaaccc
    16921 cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct gagcgtcgta
    16981 gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc
    17041 atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat ctgccagcag
    17101 atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg aagtaatcac
    17161 attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca accagcggcg
    17221 cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc
    17281 ggcggcttcg aagatgcggt gtttgttggc tggatggtct aacagcgcca ccagatagtg
    17341 cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa
    17401 cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac
    17461 gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa tgtccgccgt
    17521 agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca acgaactgag
    17581 aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga gagccacctg
    17641 gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa aatagacggt
    17701 atcgatatcc tgcaacaggg caggaaggtt atccggccag ctgagatcga ctttatggca
    17761 actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgccaggat
    17821 ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc
    17881 gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat gcgcgccagt
    17941 gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa
    18001 tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac gcggcagcat
    18061 tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg
    18121 cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga
    18181 cgttattttt cagggcatac atcccggcgg cagccagaat cccggactga cgcatcccgc
    18241 caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta ccgacgagta
    18301 atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt
    18361 gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg tgcgcaccgt
    18421 caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc aggtattcac
    18481 gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat
    18541 ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag
    18601 ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca
    18661 gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag tgactgagca
    18721 gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa
    18781 gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc
    18841 caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta acggtatcac
    18901 tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta
    18961 gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata attgcgcgca
    19021 acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaatt
    19081 cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag gcgcgttgca
    19141 gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg cacgcttcgg
    19201 caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa cctccggcct
    19261 tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt ttcaccggca
    19321 gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc
    19381 acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc tgcggcatgg
    19441 cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttca
    19501 gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg
    19561 cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt
    19621 tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa
    19681 acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt gatttgatat
    19741 cgccgaccag cgccatatct accttgctat gtgcgccgat gctggctgga ttaatatcaa
    19801 tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga gtaccgagta
    19861 gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg attaacccgg
    19921 tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa
    19981 caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc
    20041 cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc agtttgcgta
    20101 actcttcttc ttccggcgtc acgactggct gtggcgcatg ataccagtgc gtggttgccc
    20161 cttctggcgc aggttttaac gccacgtcgc ccggtaacac aacaacggaa acgccacggt
    20221 taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc gggctggaaa
    20281 ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat
    20341 agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc ggaacgttat
    20401 tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg cacgatccgg
    20461 cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac gccgccactt
    20521 cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg tcgctaagac
    20581 cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat tcgagtgttt
    20641 tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg
    20701 taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg
    20761 gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat
    20821 ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca
    20881 gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta cagcagccgc
    20941 aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc gcctccagta
    21001 gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg aatttcagac
    21061 cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc
    21121 cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca
    21181 cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgaga cgaccagcga
    21241 taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta cgccactcat
    21301 cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg
    21361 gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgccg
    21421 ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtccg
    21481 aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag ccgacaccgt
    21541 catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg
    21601 tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat tgcccggcgc
    21661 gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgcg
    21721 taatgtgatg aacctgcatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac
    21781 tccttacgcg ctcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc
    21841 gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaactaggtg cagtcgggcc
    21901 ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa tcgctttctg
    21961 ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc ccagtttctc
    22021 tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac ctgcatctac
    22081 cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca
    22141 ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg
    22201 gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg
    22261 gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac caaagcccac
    22321 ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat
    22381 gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa cgatgctgcg
    22441 ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat
    22501 cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc cgctgccgta
    22561 gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc
    22621 gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc
    22681 tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga cgttgacctg
    22741 ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt
    22801 gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca
    22861 ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt cgtattgacc
    22921 gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag
    22981 acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc
    23041 tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt
    23101 atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc
    23161 aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagggt
    23221 tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg
    23281 cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc
    23341 agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg
    23401 agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt
    23461 taatatacat gtttaaggtt aagacgctta acgcggggat aaaagggatt tttcatgcaa
    23521 ctttaaggga gattgattta gcgcaatttt ggcggcaggg atctaccgcc agagaggtat
    23581 tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca
    23641 attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc
    23701 attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat cagcccaggg
    23761 atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc gctcccgatt
    23821 accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc gaaaccggag
    23881 gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat taaaccacca
    23941 attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc
    24001 tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt
    24061 aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg
    24121 gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat cgcaatacgc
    24181 gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc cagccacatc
    24241 agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc
    24301 gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat aaaaaagaga
    24361 ataaggtaaa ccatccagct taatagctga ttaataactt ttaacgcagc ttgttggcga
    24421 agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc agaaaacatg
    24481 aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac tgaaagcgga
    24541 aggacgagta aagttgcaat taaaaggaaa tgttatgcat cagaggggga tttttaatcc
    24601 tatctgagtg tttagcattg ttgcgctcct ttcatgtgca atgccatata aagccactgg
    24661 cggagggagc gcgcaggggg cagccagtcg ccgccgcccc ctgcaccccc gggctctggc
    24721 gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc ttcggcaacc aggtcgggcg
    24781 cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc atgtgggctg ccccggcctt
    24841 cggggaacgc ttcagcgatt ttgacgccac caatacccgt gcggtttgta atgtcgaaga
    24901 taaaacaacc agggcagata aaatgccaat gctaattaat cacgcttttc cagcagagtc
    24961 cggtaaatca gaccaccgat aatgccgccg acaattggca ccacccagaa gaaccacagt
    25021 tgttctaatg cccagccgcc ctggaagata gcaaccgcgg tgctgcgcgc cgggttaaca
    25081 gaagtgttag tcaccggaat actgattaag tgaatcaggg ttaaggccag accaatagcg
    25141 atcggcgcaa aacctgccgg agcgaatttg tcggttgcac cgtggatcac taacaggaag
    25201 cccgcactca ataccagttc aactaccagc gcggaaagca tggaataacc gcctggtgaa
    25261 tgctcgccat aaccgttaga agcaaaaccg ctggctgccg cgtcaaaacc cgttttacca
    25321 ctgacaatta aatacagcag cgccgctgca acaataccgc cgacaacctg ggcaatcacg
    25381 tagccaacga cttcttttgc cggaaaacgt ccgccagccc ataaaccaat agtgaccgcc
    25441 gggttaaaat gaccaccaga aatatgacca acagcgaagg ccatcgtcag aacggtcaaa
    25501 ccaaatgcca gcgccacgcc ggcaaaacca atgcctaatt ccgggaagcc tgcggccagt
    25561 acagcactac cacagccacc aaaaacaagc cagaaagtac caaaacattc agctgctaat
    25621 tttctgaaca tatccacctc aattaaaaat tgaccctgtg aaaaataggg tcgttttata
    25681 gggtcgtcgt aaaaaatgac gacggaaata atgcgcggct attttaaaaa cgaaggcgag
    25741 tcattcacca gataaataaa tccagtaaat ttgatttagg gcaacagcgg gttgccccat
    25801 atagtcattt gtctgattga cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa
    25861 taaaaaataa tttatcatgc taattatttg attttgttgt ttttgcgggc ttatcagcaa
    25921 gagggagtat aacgcgatta ttcgctcatt tttcagacaa ttgccatgct taaatgtgat
    25981 gtcatcacgt attagcaagg cctttcccgt tatactgcca gcgtaaagga taagtcacat
    26041 atttctggag gggatatgat tcttgagcgc gttgaaattg tgggttttcg cggtatcaac
    26101 cgtttgtcgt tgatgctcga acaaaacaac gtcctgattg gggagaacgc gtggggtaaa
    26161 tccagcttgc tggacgcctt aaccctgctg ctatcgccag aatcagatct ctaccatttt
    26221 gagcgcgacg atttctggtt cccgccggga gatatcaacg ggcgagaaca tcatctgcat
    26281 attattttga ccttccgcga atcgctgcca ggccgacatc gggttcgccg ttatcggccg
    26341 ctggaagcgt gctggacgcc atgcaccgat ggctatcacc gtatttttta tcgtctggaa
    26401 ggggagagtg cggaagacgg cagcgtgatg acactgcgca gttttctcga taaagacgga
    26461 catccgattg atgttgagga tattaacgat caggcacgcc atctggtgcg tttaatgccg
    26521 gtgctgcgct tgcgtgatgc ccgttttatg cgccgtattc gtaacggcac ggtgccaaat
    26581 gtccccaatg tggaagtcac cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca
    26641 catccgcaaa atctctctga tggacagatt cgtcagggac tttccgcaat ggtgcagttg
    26701 cttgagcatt atttctctga gcagggggct ggacaggcgc gatatcgttt aatgcggcgg
    26761 cgggccagca atgagcaacg aagctggcgc tatctggaca tcatcaaccg gatgattgac
    26821 cgacctggag ggcgctcgta tcgggttatt ttgctcggcc tgtttgctac tttattgcag
    26881 gcaaaaggca cattgcgact ggataaagac gcgcgtccgt tattgctaat tgaagatcca
    26941 gaaacccgcc tgcatccgat tatgctttcc gttgcctggc atctgttgaa tcttttgcct
    27001 ttacagcgta ttgctaccac caactctggc gagctgcttt cgttaactcc agtggaacat
    27061 gtttgccgcc tggtgcgtga atcttctcgt gttgccgcct ggcgtctggg gccaagtggc
    27121 ttgagcaccg aagacagccg ccgcatctct tttcacattc gttttaatcg tccgtcatcg
    27181 ctgtttgctc gctgctggtt gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa
    27241 ctggcgcgtc agtgcggaca tcattttgat gccgaaggga tcaaggtcat tgagtttgcc
    27301 cagtccgggc taaagccact ggttaaattt gcccgccgaa tggggattga atggcatgta
    27361 ctggtcgatg gcgatgaagc agggaagaaa tatgccgcta cggtacgcag cctgttgaat
    27421 aacgatcggg aagccgaacg agaacattta acggcgttac cggcgctgga tatggaacat
    27481 tttatgtatc gccagggatt ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat
    27541 gtaccgatga atctacgcaa aattatctcg aaagcgatcc atcgctcttc caaacccgat
    27601 cttgccattg aagtggcaat ggaggcagga cgtcgtggtg tggactccgt accaacgctg
    27661 ctgaaaaaaa tgttctcacg cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga
    27721 aacatcgtgg ccatttgtgg ctgaatagcg tcgagcatct catagcgccg acggtattca
    27781 gcccgttttt tactggcgat ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg
    27841 taatggcgtt cagcatcaca tacgccgcca accgactccc agaacgcgtt gtaatcagca
    27901 tggatcttgc cttctttatc gcgataacgc aggctgcggt aaatatgcgt ttcattgctg
    27961 acggcaataa tctgctctac ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg
    28021 aggcgtttgg gaaatagccc gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga
    28081 atttcccatt ttgcgccttg cagtccgcca ataaacatcg ttcttttccc ctgatattca
    28141 cacagggtaa acgtgatctc tgccagagga ataccttcgc tgttgcggaa caggattgtg
    28201 ctgtcacctt ctttatccat tgagatcatc atggtcagct caagcgtgaa ctgctcaccg
    28261 tttttgcctt ccagtttagc cagttgcagc ccgggggtat tcaaatataa gctgaattct
    28321 tccgccgaca tacacccgcg gagtaacgca taatggtaac gtaacgcctc cagcaattgc
    28381 ttacggctaa gattcgccgc aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg
    28441 cgcgttaaca acacattgag attaggccag tgggaaagtt cgttcatcca ctcaacgctt
    28501 aaacgcggca taatcaacga gcgcagcaaa aatttctggc gaaaactaca gcgatgccag
    28561 aatttacccg gccgacactg tccacgtgcc agactaagaa aaagtgacag gctgctgaga
    28621 gattcagatg gcgtaaaggt ccgttcagtt agctgcgaca tattcatgaa atcaatggtt
    28681 atacatgacg tcgatttcac cattgcgtat cttaaccaaa catcaatagt gtgattacta
    28741 acgtaaattt tagggttttg ttgatatttc gttgaagtta atgacccgga ttggcatgtg
    28801 gagtattcag aatatttatg aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc
    28861 tggtgatagt catcgccgga ctgattacgt tatggagaat tcttaacgca cccgtgccga
    28921 cttatcagac actgattgtg cgccccggtg atttacagca aagcgtgctg gcgaccggaa
    28981 agctggacgc gctgcgtaag gttgacgtag gcgcgcaggt cagcggtcag ttgaaaacgc
    29041 tgtcggtggc gattggcgat aaagtaaaaa aagaccagct tttaggggtc attgatcctg
    29101 aacaggctga aaaccagatc aaggaggtcg aagcaacgct gatggagcta cgcgcgcagc
    29161 ggcagcaggc ggaagcggag ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc
    29221 tggcacaaac gcaagcggtt tcactgcagg atctcgatac cgccgcgacg gagatggctg
    29281 tgaaacaggc gcaaattggc accattgacg cgcaaatcaa gcgcaatcag gcctctctcg
    29341 atacggctaa aaccaatctc gattacaccc gtatcgttgc cccgatgggc ggggaagtga
    29401 cgcaaatcac cactctgcaa ggccagacgg tgattgccgc acagcaagca ccgaacattc
    29461 tgacgctggc agatatgagc actatgctgg taaaagcgca ggtttctgaa gcggatgtaa
    29521 tccacctgaa gccggggcaa aaagcctggt ttacggtgct tggcgatcca ctgacgcgct
    29581 acgaggggca aatcaaggat gtgctaccga cgccggaaaa ggtaaacgac gctattttct
    29641 attacgcccg ttttgaagta cccaacccca atggtttgct gcggctggat atgaccgcgc
    29701 aagtgcatat tcagctcacc gatgtgaaaa atgtgttgac gatccctctg tcggcgttag
    29761 gcgatccggt tggcgataat cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg
    29821 agcgtgaagt gacgattggc gcacgtaacg ataccgatgt tgagattgtc aaagggctgg
    29881 aagcgggcga tgaagtggtg attggtgagg ccaaaccagg agctgcacaa tgacgccttt
    29941 gctcgaatta aaggatattc gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct
    30001 gaagggcatc agcctcaata tttatgcggg tgagatggtc gcgattgttg gcgcttcggg
    30061 ttccggtaaa tcgaccctga tgaatattct cggctgcctg gataaggcca ccagcggcac
    30121 ctatcgcgta gccggtcagg atattgccac gctggacgcc gatgcgctgg cgcaactgcg
    30181 ccgcgagcat ttcggcttta ttttccagcg ttaccatttg ctttcgcatt taaccgccga
    30241 gcagaacgtt gaagtacccg ccgtctatgc cggtcttgag cggaaacagc gactgcttcg
    30301 tgcccaggag ttgctgcaac ggctggggct ggaagaccgt acagagtatt atccggcaca
    30361 gctttcgggt ggtcagcaac agcgcgtcag catcgcgcgg gcattgatga acggcggtca
    30421 ggtaattctt gccgatgaac cgaccggcgc actggacagc cattctggcg aagaggtgat
    30481 ggcgatcctg catcagctgc gcgatcgcgg gcatacggtg attatcgtca cccacgatcc
    30541 gcaggtcgct gctcaggccg agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa
    30601 tcctcccgcc attgaaaaag tgaatgttgc tggcgggacg gagcctgttg tcaatacagt
    30661 gtctggctgg cggcagtttg tcagcggttt taacgaggcg ctgacgatgg catggcgggc
    30721 gctggcagcg aataaaatgc gtactttact gaccatgctg gggattatta tcggtattgc
    30781 gtcggtggtt tccattgtcg tggtgggtga cgccgccaaa caaatggtgc tggcggatat
    30841 tcgttctatt ggtacgaata ctattgatgt ctatcccggg aaagattttg gcgatgacga
    30901 tccgcaatat cagcaggcgc tgaagtacga cgacttaatc gccatccaaa aacaaccgtg
    30961 ggtcgcctca gccacacctg ccgtctcgca aaacctgcgc ctgcgttata acaatgttga
    31021 tgttgctgcc agtgccaatg gcgtgagcgg cgattatttt aatgtctatg gcatgacctt
    31081 cagtgaagga aacaccttta atcaggagca gctgaacggt cgtgcgcagg tcgtggttct
    31141 cgacagtaat actcgccgcc agcttttccc ccataaagca gatgtggttg gcgaggtgat
    31201 tctggtcggc aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt
    31261 tggtagcagt aaagtgctgc gtgtctggct accttacagc acgatgtccg ggcgagttat
    31321 gggccagtcg tggcttaact ccattactgt cagggtgaaa gaaggatttg acagcgccga
    31381 ggcggaacag caactcacgc gtttactttc actgcgccac ggtaagaagg atttctttac
    31441 ctggaacatg gacggcgtct tgaaaactgt tgaaaagacc acacgtactt tacaactgtt
    31501 tctgacgctg gtggcggtga tttcgctggt ggtgggcggt attggtgtaa tgaatattat
    31561 gctggtgtca gtgaccgagc ggacgcggga aattggcatt cgcatggctg taggtgcgcg
    31621 agcaagcgat gttttgcaac agttcctgat cgaagccgta ctggtttgcc tggtcggtgg
    31681 cgcgttggga ataacactgt cactgttaat tgctttcacc ttgcagcttt tcttacccgg
    31741 ctgggagatt ggtttttcac cgttggcgct gctgctggcg tttctctgct cgacggccac
    31801 cgggatttta tttggctggt tacccgcacg aaatgcggca cgactggatc cagtagatgc
    31861 tctggcacgg gagtaatttt tgagataaaa atgccagccg atcgggctgg cattttgcct
    31921 ttaggatgta cacaatgaga cagaagagct atgcgactgc cgcttctact tcgacgggca
    31981 caataacact ggcgtgattg ccttttggcc cctggtggac atcaaactga acggattgtc
    32041 cagcttttag cgttctgtaa ccatccatct gaatggtgga ataatgagcg aaaatatctt
    32101 cgccgccgcc ttcagggcag atgaaaccaa aacctttggc attgttgaac cacttaacag
    32161 tacccttttc catgcttcga catccttcgc aaatcttata caagtaagat ggaataaacc
    32221 ggggtcagag agggggctgt tcaaaacctc gccaactcta gaaatacaat ttagagaatt
    32281 agggcgagcc gtcaagcatt tgacaggggc cagggggcag gtatgaatca aaaatttgaa
    32341 gcagttaacg ctattgacag gaatgtgaca gatgtcgctg atgccaacga tagatgatag
    32401 ttatctatca tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggcgtt
    32461 ctgccgataa ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg
    32521 actttgatca actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata
    32581 aagtgatatt agtcaatgat gattacactc cgatggagtt tgttattgac gtgttacaaa
    32641 aattcttttc ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg
    32701 ggaaggccat ttgcggagtc tttaccgccg aggttgcaga aaccaaagtg gcgatggtga
    32761 acaagtacgc gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc
    32821 aggcataaaa attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata
    32881 tggctttcgc cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac
    32941 tggcgctgct cagtaaccca tctgcccggg aggcgctgga agcgtgttct gtggatttgg
    33001 ttgcgctccg tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca
    33061 gtgaagagga gcgcgacaca cagccgacgc tgagttttca gcgtgtactg caacgtgcgg
    33121 tcttccatgt ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta
    33181 tcttcagcga acaggagtcg caggcggcat atctgttgcg taaacatgaa gtcagccgtc
    33241 tcgatgtggt gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg
    33301 atcctggcag ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atggagaatt
    33361 tcacgacgaa cctgaaccag cttgcgcgtg tgggcggaat cgacccacta attggtcgtg
    33421 agaaagagct ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc
    33481 tggtggggga atctggtgtc ggtaaaaccg cgattgcaga aggtcttgcc tggcgaattg
    33541 ttcagggtga tgtgccggaa gtgatggctg actgtacgat ttactctctc gatatcggtt
    33601 ctctgttagc gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa gcgttgctca
    33661 agcagctgga gcaggacact aacagcatcc tgtttattga tgagatccac accattatcg
    33721 gtgcgggtgc agcgtctggt ggtcaggtcg atgcggctaa cctaatcaaa ccgttgctct
    33781 ccagcggtaa aattcgcgta attggttcga caacctatca ggagttcagc aacattttcg
    33841 agaaagaccg tgctctggcg cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg
    33901 aagaaactgt tcaaatcatc aatggcctga aaccgaagta tgaagcgcac cacgacgtgc
    33961 gttataccgc aaaagcggtg cgtgcggcgg tagagctggc ggtgaaatac attaacgatc
    34021 gtcatctgcc ggataaagcc attgatgtta tcgacgaagc gggcgctcgc gcacgtctga
    34081 tgccggtaag caaacgcaag aaaaccgtta atgtggcgga tattgagtcc gtggtggccc
    34141 gtattgcacg cattccagag aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc
    34201 tcggcgatcg cctgaaaatg ctggtcttcg gccaggataa agccattgag gcgctgactg
    34261 aagccattaa gatggcgcgt gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc
    34321 tgtttgccgg tcctaccggg gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt
    34381 tgggcattga gcttctgcgc tttgatatgt ccgagtatat ggaacgccat accgtcagcc
    34441 gtctgattgg tgcgcctccg ggatacgttg gttttgatca gggcggtttg ctgaccgatg
    34501 cggtaatcaa gcatccacat gcggtgctgc tgctggacga aatcgagaaa gcgcatccgg
    34561 acgtgttcaa tattctgttg caggtgatgg acaacggtac gctgaccgat aacaacggac
    34621 gcaaagcgga cttccgtaac gtggtgctgg tgatgaccac taacgccggg gtacgggaaa
    34681 ctgagcgcaa atccattggt cttatccacc aggataacag caccgatgcg atggaagaga
    34741 tcaagaagat ctttacaccg gaattccgta accgtctcga caacattatc tggttcgatc
    34801 atctgtcaac tgacgtgatc catcaggtgg tggataaatt catcgtcgag ttgcaggttc
    34861 agctggatca gaaaggtgtt tctctggaag tgagccagga agcgcgtaac tggctggccg
    34921 agaaaggtta cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc caggacaacc
    34981 tgaaaaaacc gctcgccaac gaactgctgt ttggttcgct ggtggacggc ggtcaggtga
    35041 cggttgcgct ggataaagag aaaaatgagc tgacttacgg attccagagt gcacaaaagc
    35101 acaaggcgga agcagcgcat taatctgatt gtcaggtagg ttggtgaagt ccgtaatctc
    35161 gtcaggggtt acggactttt tatttatggg gtggaacggt gaaaacccta tttttggagg
    35221 tgaaggtaag ttgttgataa ttagtgctgc tggaaggtaa ggataaaaaa gggtgctgca
    35281 ggagaatggg atggttttgc tttattaaca acgggctaaa cgtgtagtat ttgagttcac
    35341 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
    35401 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
    35461 tgccgtacag acagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
    35521 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
    35581 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
    35641 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
    35701 taattattgt ttgctcatac tgtaaacaac ttgtgcagta tatctacatc gagacaagtt
    35761 atggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
    35821 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
    35881 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
    35941 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
    36001 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
    36061 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
    36121 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
    36181 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
    36241 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
    36301 ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
    36361 cgaaaacatt cattcttgtg gtgaaaacaa gcaccgtggt acccagaaat tattcggcaa
    36421 tcgtccgagg cgcatttgat tgagataatt aaggtaatcc cggcggggaa tttcgcaggc
    36481 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgacc
    36541 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
    36601 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
    36661 atcttcacgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc
    36721 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat
    36781 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
    36841 catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat cgggcgacca
    36901 ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
    36961 caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct cacgtaatgc
    37021 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
    37081 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt gaactggtaa
    37141 taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
    37201 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
    37261 accgttttct cacgcatcat ttttgcaagc aattcaagga tctggctttc ggttgtggca
    37321 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
    37381 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
    37441 ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac
    37501 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    37561 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    37621 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc
    37681 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc tatatgttcc
    37741 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    37801 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    37861 aaggtgacct ccggtttttg atccgttaag tcagagatac gtacggcaga ggcaatgact
    37921 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgca
    37981 caaaagacaa acagggcaat taatgcgccg ggttgagcat tgccgccaac gccgccagac
    38041 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    38101 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    38161 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    38221 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    38281 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aacgctgccc
    38341 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc
    38401 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    38461 tccgccacca cgcggttgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    38521 aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    38581 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc
    38641 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgcaaccgct
    38701 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca
    38761 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    38821 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
    38881 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatccggcc atcctgcata
    38941 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    39001 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    39061 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
    39121 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggtgtatca
    39181 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    39241 aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa ttttgctgcc
    39301 ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    39361 tcacgtaatt ctatcccgtt gattcgtaat gatccctgat atgagagaaa accagaaagc
    39421 gcgttcagca gtgagctttt acctgaaccg ctgcggccaa ccaacactgc acgttggcct
    39481 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    39541 agatcctcgg cctcaatggt cagcggatcg gtcaatgcta attccgcctc accgcgctgc
    39601 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    39661 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg
    39721 atcagggcca gaaaacctgc agccagcgtc accccagtat cgtagtgacc aaaatccagc
    39781 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    39841 aattcgagaa tgccggagga taaaaacgcc agtcgtagca cttccattgt ccgttggcgg
    39901 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    39961 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    40021 aagttacgcc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    40081 agcggtgctg tgcccagcag aatgagcgcc gcagcccagt tagaggggaa gatcgccacc
    40141 acaatcagca acggcaccga cactgccagc gccatttgcg gcagatagcg tgcatagtaa
    40201 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc
    40261 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    40321 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc
    40381 aatacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    40441 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    40501 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    40561 tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt
    40621 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    40681 agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    40741 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag
    40801 taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc
    40861 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    40921 ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg
    40981 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    41041 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    41101 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    41161 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    41221 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    41281 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    41341 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    41401 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    41461 tttcaaactt ggtggcatgt tcgtgcatgc gcgccattaa taacggaccg gtcagatcgt
    41521 ttggatcacc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    41581 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    41641 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    41701 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    41761 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    41821 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    41881 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa attgatgttt tgctttgaca
    41941 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    42001 ataaccatgt atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    42061 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    42121 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    42181 cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca ataataatgg
    42241 tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    42301 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    42361 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    42421 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    42481 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    42541 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    42601 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    42661 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    42721 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    42781 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    42841 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    42901 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    42961 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    43021 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc
    43081 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    43141 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    43201 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    43261 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    43321 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    43381 agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    43441 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    43501 cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca
    43561 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    43621 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    43681 ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc cggacgacgt
    43741 cctatttttg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    43801 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cgacacaaag
    43861 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    43921 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    43981 tattgctcct gcaccggaag gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca
    44041 atataatgag ccgctgcaac aaccagtaca gccgcagcag ccgtattatg cacctgcagc
    44101 tgaacaacct gcgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    44161 gtattacgca actgcgccgg aacagcctgc gcaacagccg tattatgccc ctgcgccaga
    44221 acaaccggtg gcaggtaacg cctggcaagc cgaagagcag caatccattt ttgctccaca
    44281 gtctacatac cagactgagc aaacttatca gcagccagcc gctcaggagc cgttgtacca
    44341 acagccgcaa cccgttgaac agcagcctgt tgtggagcct gaacccgttg tagaagagac
    44401 aaaacccgcg cgtccgccgc tttactattt tgaagaagtg gaagagaagc gagcccgtga
    44461 acgtgaacaa cttgcggcct ggtatcaacc gattccagaa ccggttaaag aaccagaacc
    44521 gatcaaatct tcgctgaaag caccttctgt tgcagcagta cctccagtag aagccgctgc
    44581 cgctgtttct ccgctggcat ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc
    44641 aaccgttgcc gcgccagtct tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa
    44701 agaggggatt ggcccgcagt tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga
    44761 attggcgtct tacggtatta agctgccctc acagcgtgcg gcggaagaaa aagcccgtga
    44821 agcccggcgc aatcagtatg attctggcga tcagtataac gatgatgaaa tcgatgcgat
    44881 gcagcaggat gaactggcac gtcagttcgc ccagacacag cagcaacgct atggcgaaca
    44941 gtatcaacat gatgtgcccg taaacgcaga agatgcagat gctgcggcag aggctgaact
    45001 ggctcgtcag tttacgcaaa ctcaacaaca acgttattcc ggcgaacaac cggctggggc
    45061 gaatccgttc tcgctggatg attttgaatt ttcgccaatg aaagcgttgc tggatgatgg
    45121 tccacacgaa ccgctgttta cgccaattgt tgaacctgta cagcagccgc aacaaccggt
    45181 tgcaccacag cagcagtatc agcagccaca acagccggtt gcgccacagc cgcagtatca
    45241 gcagccacaa cagcaggttg cgccgcagcc acaatatcag cagccgcaac aaccggttgc
    45301 gccacagcag caatatcagc agccgcaaca accagttgcg ccgcagccgc agtatcaaca
    45361 gccgcaacaa ccggttgcac cgcagccgca gtatcagcag ccacaacagc cagttgcgcc
    45421 acagccgcag gataccctgc ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt
    45481 gcacaaaccg acgacgccgc tgccttctct ggatttgctg acaccgccgc cgagcgaagt
    45541 ggagccggta gatacctttg cgcttgaaca aatggcgcgt ctggtggaag cgcgtctggc
    45601 tgatttccgt attaaagccg atgtcgttaa ttactctccg gggccagtta tcactcgctt
    45661 tgaacttaac ctggctcctg gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct
    45721 tgcccgttca ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta
    45781 tgtaggtctg gagttaccaa ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga
    45841 taacgccaaa ttccgcgata atccgtcgcc attaaccgtg gtgctgggta aagatatcgc
    45901 cggtgagccg gtggttgccg atctggcgaa aatgccgcac ttgttggttg cggggactac
    45961 cggttccggt aaatctgtcg gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca
    46021 gccagaagat gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt cggtttatga
    46081 aggcattccg cacctgttaa cggaagtcgt tactgatatg aaagatgccg ccaacgcgct
    46141 gcgctggtgt gttaacgaga tggagcgtcg gtataaactg atgtctgcgc tgggtgtgcg
    46201 taatctggcg ggttataacg aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc
    46261 agacccgtac tggaagccgg gtgacagtat ggatgcccag catccggtgc tgaaaaaaga
    46321 accatacatt gtcgtgttgg ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa
    46381 agtggaagag ctgatagcac gtctggcgca aaaagcccgt gccgcgggta tccacctcgt
    46441 actggcaact cagcgtccat cggttgatgt tattactggt ctgattaaag cgaatattcc
    46501 gacccgtatc gcctttaccg tatccagtaa gattgactca cgtaccattc ttgatcaggc
    46561 tggcgcggaa tcactgctgg gtatggggga tatgctctac tctgggccga actccacgtt
    46621 gccggtacgt gtccatggtg cttttgttcg cgatcaggaa gttcatgccg tggtgcagga
    46681 ctggaaagcg cgtggtcgcc cacagtatgt tgatggcatc acctccgaca gcgaaagcga
    46741 aggtggtgcg ggtggtttcg atggcgctga agaactggat ccgttgttcg atcaggcggt
    46801 gcagtttgtc actgaaaaac gcaaagcgtc aatttctggc gtacagcgtc agttccgcat
    46861 tggttataac cgtgcagcgc gtattatcga acagatggaa gcgcagggga ttgtcagcga
    46921 acaggggcac aacggtaatc gtgaagtgct ggccccaccg ccgtttgact aattaatgca
    46981 ttgccggata aggcgcggta gcgtcgcatc cggcactcta tcaactgaaa attcagtatt
    47041 ttcttctttc ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccgatcg
    47101 ggagtgacgt aatttgagga ataatgatga aaaaaattgc tatcacctgt gcattactct
    47161 caagcttagt agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agccgcctgg
    47221 ataaagtcag cagcttccac gccagcttca cacaaaaagt gactgacggt agcggcgcgg
    47281 cggtgcagga aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata
    47341 tgacacaacc tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc
    47401 cgttcgttga gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta
    47461 tgctgattgc ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg
    47521 atgactttgt cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg
    47581 tgggacgtga tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca
    47641 gttatcagct gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc
    47701 cgccgcaagg cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg
    47761 tcgctcgatt tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat
    47821 ttagcacagt atatcggtca gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct
    47881 atcgaagccg ggcatttgca ttctatgatc ctctgggggc caccgggtac cggcaaaaca
    47941 accctcgctg aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc
    48001 acctctggcg tgaaagagat tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca
    48061 ggtcgccgca ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat
    48121 gcatttctgc cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac
    48181 ccgtcgtttg agcttaattc ggcactgctt tcccgtgccc gtgtttatct gttgaaatcc
    48241 ctgagtacag aggatattga gcaagtacta actcaggcga tggaagacaa aacccgtggc
    48301 tatggtggtc aggatattgt tctgccagat gatacacgac gtgccattgc agaactggtg
    48361 aatggcgacg cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc
    48421 tatgatagcg gtaagcgggt cctgaagcct gaattactga ccgaaatcgc cggtgaacgt
    48481 agcgcccgct ttgataacaa aggcgatcgc ttttacgatc taatttccgc actgcataag
    48541 tcggtacgtg gtagcgcacc cgatgcggcg ctgtactggt atgcgcgaat tattaccgct
    48601 ggtggcgatc cgttatatgt cgcgcgtcgc tgtctggcga ttgcgtctga agacgtcggt
    48661 aatgccgatc cacgggcgat gcaggtggca attgcggcct gggattgctt tactcgcgtt
    48721 ggcccggcgg aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca
    48781 aaaagcaacg ctgtctacac tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgcccg
    48841 gattatgacg tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc
    48901 tacgggcagg aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt
    48961 tacttcccgc cggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa
    49021 ggcaagattg gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa
    49081 cgctaccgtt aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct
    49141 gtggccacat ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg
    49201 ctgcgtaatg agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat
    49261 gtagataagc tgggcgctct tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac
    49321 ctgcaagcgg agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa
    49381 gatatcgagc ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa
    49441 gccgagctag atgctttaca ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg
    49501 cctgcagatg aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagccgctgg
    49561 ggtaccccgc gtgagtttga ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac
    49621 tctggcctcg actttgcagc tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa
    49681 gggcagattg ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa
    49741 cagcatggct acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt
    49801 acgggtcagc tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa
    49861 gcagacacca gtaactatgc gctgatccca acggcagaag ttccgctgac taacctggtg
    49921 cgcggtgaaa tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgc
    49981 ttccgttctg aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag
    50041 ttcgacaaag ttgaaatggt gcagatcgtg cgcccagaag actcaatggc ggcgctggaa
    50101 gagatgactg gtcatgcaga aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc
    50161 atcctttgca ctggcgacat gggctttggc gcttgcaaaa cttacgacct ggaagtatgg
    50221 atcccggcac agaacaccta ccgtgagatc tcttcctgct ccaacgtttg ggatttccag
    50281 gcacgtcgta tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat
    50341 accctgaacg gttctggtct ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat
    50401 cagcaggctg atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg
    50461 gaatatattg gctaataccc aatttttctg aatctaaaaa gcgcctttag ggcgcttttt
    50521 ttgtctccct ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga
    50581 gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag cctatatagc
    50641 gagctgctat agaaataatt acacaatacg gtttgttact ggaatcaatc gtgatcaagc
    50701 ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggagagtc
    50761 gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa
    50821 cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcagacg
    50881 aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc
    50941 acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg
    51001 acggcctgca ctaggttcgc gcctgcctgc gtgggcgttc catgcgtcgc cgtgtctaca
    51061 atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg
    51121 agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca
    51181 aagagtacgg caacgagtct atctatctga actatggcac cggtacgctg ggcggcacca
    51241 tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg
    51301 gttatcttaa ccattacggt gactactctt ccgcgcaaat tgcggaaggt ttgaaccata
    51361 cctacggcgg ctgggcagat ggtaacagcc cgtcggatat cgaaaacagt aaattggtag
    51421 tgctgtttgg taataaccca ggcgaaacgc gaatgagtgg cggtggggtg acttactatc
    51481 ttgaacaggc acgccagaaa tctaatgctc gcataatcat catcgatccg cgctataccg
    51541 acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccaggaaca gatgccgcac
    51601 tggttaacgg tctggcgtac gtcatgatca ctgaaaacct ggtggatcag gcattcctcg
    51661 ataaatattg cgttggctat gacgagaaaa ccctgcccgc cagtgcgccg aaaaatggtc
    51721 attataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaaca ccggaatagg
    51781 cctcgcaaat caccggtgtt ccggcagaca aaatcatcaa actggctcgt gaaatcggca
    51841 gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct aacggtgaaa
    51901 tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt attaatggag
    51961 ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg ccgaccttgg
    52021 aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc
    52081 cggaaatgac ggcgctgcgt gatggtgtgc gcgggaaaga taagctggat gtgccgatca
    52141 aaatgatctg gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta
    52201 cccatgaaat cctccaggat gataagaagt gcgagctgat tgtggttatc gactgccaca
    52261 tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga
    52321 tggactttgc actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg
    52381 tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac
    52441 gtcttggcgt tgagcaacag tttactgaag gccgtaccca ggaagagtgg atgcggcatc
    52501 tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc
    52561 aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa gccttccgtg
    52621 aagatccgca ggcaaaccca ctgactacgc catcgggcaa aattgagatt tattcgcagg
    52681 cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc
    52741 cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc
    52801 agcttacagg tttccactat aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc
    52861 tgaaagcggc ttgccgtcag gaaatgtgga tcaacccgct tgatgcccaa aaacgcggta
    52921 tccacaacgg cgataaagtc aggatcttta acgatcgtgg tgaggttcat attgaggcga
    52981 aagtgacgcc acgaatgatg ccgggcgtgg tcgcactggg tgaaggcgcc tggtatgacc
    53041 cggatgcaaa acgtgtcgat aagggcggtt gtatcaacgt actgaccact caacgtccgt
    53101 ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag
    53161 gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc
    53221 aaaacctgcg agctggcttg taaagactac aaagatttga cgccagaagt cagtttccgc
    53281 cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg gcaccagaac
    53341 gtgtttgcct actatctgtc gatttcgtgc aaccactgcg aagatccggc ttgtactaaa
    53401 gtctgcccga gcggtgcgat gcataaacgc gaagatggtt ttgttgtggt cgatgaagat
    53461 gtatgcattg gctgccatta ctgtcatatg gcttgcccgt atggcgcacc gcaatataac
    53521 gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa
    53581 aagccgatct gtgttgaatc ttgcccgctg cgggcgctgg atttcggccc tatcgacgag
    53641 ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc
    53701 aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgacagggga taccactggc
    53761 tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat
    53821 gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt atcgttctgg ctttggcgct
    53881 gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggctt
    53941 atgggtactg atgggcattg gctttatcgc ctctatgctc catcttggtt caccgatgcg
    54001 cgcctttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg
    54061 ttcgatcttt tttgctgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc
    54121 accggcattg cgtacgctat ggctggtggt gacgatggtt cttggcgtcg tctttgtctg
    54181 gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac
    54241 gccgatgggc ttcttcctga cgatgttcat gggcggcccg ttactgggtt acctgttgct
    54301 gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagccattt ctgtactggc
    54361 actggtggtg agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag
    54421 ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat
    54481 cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc
    54541 tgcggttccg ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg
    54601 cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac
    54661 aaccggggtt tcggccccgg ttctctttta tttctgcttc gttaacgtgt cataactggt
    54721 catcaaatta cgataatcag gaatatggtt agagaacaac gtcgccagtc cttcaatatc
    54781 attacgccag tcacggtgca gctcgcaagc cacgccgaac catgtcatca attgcgcacc
    54841 agcttgcgac atgcgatccc acgccgaatg ccgggtaatt tcattaaagg tccctgatgc
    54901 atcagtcacg acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac
    54961 gcaaacttcg gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc
    55021 ttttacaaaa tcttcgttat cccaggcgtt aatattgccc gggcgagcaa tgtaaggtgc
    55081 atcggggaat tgtgctttca gttctagaac taatgggccg ttaggaccgg tttcaaaact
    55141 ggtggtaaga atagttggta agttgaaata cttagccagg tcacccagcg ccagcacgtt
    55201 atttttaaac ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctggtgatc
    55261 aacaagcaaa acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt
    55321 ctcctttcga gagtcagtgg tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa
    55381 gcatagacgg gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta
    55441 tgaaatttgt gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta
    55501 gcgttatgaa tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca
    55561 cccattgaca atgtttttgg cggtggcatg atgcgcatga aatttgaact tcctcacggt
    55621 tttaattcat gtccacgtat acccggcctg tcatgctttt actgtctggc ctgcttttgt
    55681 tgactctggc gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca
    55741 tgtccacatg gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta
    55801 cattgctgac agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctgtcct
    55861 ccttcatttt tgctgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt
    55921 tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg
    55981 cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg
    56041 tttattacgt gggaacgttt ttaggccagt tactggtcag caaagtctca accgagctga
    56101 tgagcgtatt gccgtgggtt acaggtttga cattggcagg gatcttaccg ctgttgttta
    56161 cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac gtcaattacg gcaatgctaa
    56221 aactccgtca ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gttctgggat
    56281 ctctatatgg cctgatgcct ctgtacctca atcacaaagg ggtgagcaat gccagtattg
    56341 gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc
    56401 tggcggataa gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc gtcattctcg
    56461 gcagtattgc gatgcttagc caggcggcga tggccccagc gttattcatc ctcggtgccg
    56521 ctggctttac gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc
    56581 aactggtggc gatgaaccaa gccttactgt tgagctatac cgtgggaagt ctgcttggcc
    56641 cgtcatttac cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg
    56701 ccagcgtatc gtttatctat ttgctgatgc tgcttcgcaa cgccggtcat acgccgaaac
    56761 ccgttgctca cgtgtaaatg aattcaagca aagtgtgaac ttactgtttc acactctgct
    56821 tttttgtttc ttctatctga cttgcttttt tccaaatttt attcgtttaa aaataaaatg
    56881 tgcagcaggt tataattttg catttcgcta tttccgcact tcttatttgc cgcgcataat
    56941 ccctcgtttt a
//
LOCUS       DAJTYI010000010        56007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYI010000010 DAJTYI010000000
VERSION     DAJTYI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..991)
                     /gene="setA"
                     /locus_tag="OO564_000615"
     CDS             complement(<1..991)
                     /gene="setA"
                     /locus_tag="OO564_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6651863.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQ"
     gene            complement(1093..1224)
                     /gene="sgrT"
                     /locus_tag="OO564_000616"
     CDS             complement(1093..1224)
                     /gene="sgrT"
                     /locus_tag="OO564_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6651864.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1313..2968
                     /gene="sgrR"
                     /locus_tag="OO564_000617"
     CDS             1313..2968
                     /gene="sgrR"
                     /locus_tag="OO564_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6651865.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3002..3101
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3132..4115
                     /gene="thiB"
                     /locus_tag="OO564_000618"
     CDS             3132..4115
                     /gene="thiB"
                     /locus_tag="OO564_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6651866.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4091..5701
                     /gene="thiP"
                     /locus_tag="OO564_000619"
     CDS             4091..5701
                     /gene="thiP"
                     /locus_tag="OO564_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6651867.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5685..6383
                     /gene="thiQ"
                     /locus_tag="OO564_000620"
     CDS             5685..6383
                     /gene="thiQ"
                     /locus_tag="OO564_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6651868.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6497..7261)
                     /locus_tag="OO564_000621"
     CDS             complement(6497..7261)
                     /locus_tag="OO564_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6651869.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7347..8225)
                     /gene="araC"
                     /locus_tag="OO564_000622"
     CDS             complement(7347..8225)
                     /gene="araC"
                     /locus_tag="OO564_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6651870.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8564..10200
                     /gene="araB"
                     /locus_tag="OO564_000623"
                     /pseudo
     CDS             8564..10200
                     /gene="araB"
                     /locus_tag="OO564_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10211..11713
                     /gene="araA"
                     /locus_tag="OO564_000624"
     CDS             10211..11713
                     /gene="araA"
                     /locus_tag="OO564_000624"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6651871.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11913..12608
                     /gene="araD"
                     /locus_tag="OO564_000625"
     CDS             11913..12608
                     /gene="araD"
                     /locus_tag="OO564_000625"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6651872.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12683..15034
                     /gene="polB"
                     /locus_tag="OO564_000626"
     CDS             12683..15034
                     /gene="polB"
                     /locus_tag="OO564_000626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6651873.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15198..18104
                     /gene="rapA"
                     /locus_tag="OO564_000627"
     CDS             15198..18104
                     /gene="rapA"
                     /locus_tag="OO564_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6651874.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18116..18775
                     /gene="rluA"
                     /locus_tag="OO564_000628"
     CDS             18116..18775
                     /gene="rluA"
                     /locus_tag="OO564_000628"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6651875.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18892..19707)
                     /gene="djlA"
                     /locus_tag="OO564_000629"
     CDS             complement(18892..19707)
                     /gene="djlA"
                     /locus_tag="OO564_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6651876.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19962..22316
                     /gene="lptD"
                     /locus_tag="OO564_000630"
     CDS             19962..22316
                     /gene="lptD"
                     /locus_tag="OO564_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6651877.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22369..23655
                     /gene="surA"
                     /locus_tag="OO564_000631"
     CDS             22369..23655
                     /gene="surA"
                     /locus_tag="OO564_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6651878.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23655..24644
                     /gene="pdxA"
                     /locus_tag="OO564_000632"
     CDS             23655..24644
                     /gene="pdxA"
                     /locus_tag="OO564_000632"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6651879.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24641..25462
                     /gene="rsmA"
                     /locus_tag="OO564_000633"
     CDS             24641..25462
                     /gene="rsmA"
                     /locus_tag="OO564_000633"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6651880.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25465..25842
                     /gene="apaG"
                     /locus_tag="OO564_000634"
     CDS             25465..25842
                     /gene="apaG"
                     /locus_tag="OO564_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6651881.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25849..26691
                     /gene="apaH"
                     /locus_tag="OO564_000635"
     CDS             25849..26691
                     /gene="apaH"
                     /locus_tag="OO564_000635"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6651882.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26769..27248)
                     /gene="folA"
                     /locus_tag="OO564_000636"
     CDS             complement(26769..27248)
                     /gene="folA"
                     /locus_tag="OO564_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6651883.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27439..29301)
                     /gene="kefC"
                     /locus_tag="OO564_000637"
     CDS             complement(27439..29301)
                     /gene="kefC"
                     /locus_tag="OO564_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6651884.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29294..29824)
                     /gene="kefF"
                     /locus_tag="OO564_000638"
     CDS             complement(29294..29824)
                     /gene="kefF"
                     /locus_tag="OO564_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6651885.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29872..31266)
                     /locus_tag="OO564_000639"
     CDS             complement(29872..31266)
                     /locus_tag="OO564_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6651886.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31325..31612)
                     /gene="fixX"
                     /locus_tag="OO564_000640"
     CDS             complement(31325..31612)
                     /gene="fixX"
                     /locus_tag="OO564_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6651887.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31609..32895)
                     /locus_tag="OO564_000641"
     CDS             complement(31609..32895)
                     /locus_tag="OO564_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6651888.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32946..33887)
                     /locus_tag="OO564_000642"
     CDS             complement(32946..33887)
                     /locus_tag="OO564_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6651889.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33902..34672)
                     /locus_tag="OO564_000643"
     CDS             complement(33902..34672)
                     /locus_tag="OO564_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6651890.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35143..36657
                     /gene="caiT"
                     /locus_tag="OO564_000644"
     CDS             35143..36657
                     /gene="caiT"
                     /locus_tag="OO564_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6651891.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36688..37830
                     /gene="caiA"
                     /locus_tag="OO564_000645"
     CDS             36688..37830
                     /gene="caiA"
                     /locus_tag="OO564_000645"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6651892.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37959..39176
                     /gene="caiB"
                     /locus_tag="OO564_000646"
     CDS             37959..39176
                     /gene="caiB"
                     /locus_tag="OO564_000646"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6651893.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39250..40803
                     /gene="caiC"
                     /locus_tag="OO564_000647"
     CDS             39250..40803
                     /gene="caiC"
                     /locus_tag="OO564_000647"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6651894.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40912..41697
                     /gene="caiD"
                     /locus_tag="OO564_000648"
     CDS             40912..41697
                     /gene="caiD"
                     /locus_tag="OO564_000648"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6651895.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41703..42293
                     /gene="caiE"
                     /locus_tag="OO564_000649"
     CDS             41703..42293
                     /gene="caiE"
                     /locus_tag="OO564_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6651896.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42379..42774)
                     /gene="caiF"
                     /locus_tag="OO564_000650"
     CDS             complement(42379..42774)
                     /gene="caiF"
                     /locus_tag="OO564_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6651897.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43036..46257)
                     /gene="carB"
                     /locus_tag="OO564_000651"
     CDS             complement(43036..46257)
                     /gene="carB"
                     /locus_tag="OO564_000651"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6651898.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46275..47423)
                     /gene="carA"
                     /locus_tag="OO564_000652"
     CDS             complement(46275..47423)
                     /gene="carA"
                     /locus_tag="OO564_000652"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6651899.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47878..48699)
                     /gene="dapB"
                     /locus_tag="OO564_000653"
     CDS             complement(47878..48699)
                     /gene="dapB"
                     /locus_tag="OO564_000653"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6651900.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48866..49780)
                     /gene="rihC"
                     /locus_tag="OO564_000654"
     CDS             complement(48866..49780)
                     /gene="rihC"
                     /locus_tag="OO564_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6651901.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49846..50796)
                     /gene="ispH"
                     /locus_tag="OO564_000655"
                     /gene_synonym="lytB"
     CDS             complement(49846..50796)
                     /gene="ispH"
                     /locus_tag="OO564_000655"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6651902.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50798..51247)
                     /gene="fkpB"
                     /locus_tag="OO564_000656"
     CDS             complement(50798..51247)
                     /gene="fkpB"
                     /locus_tag="OO564_000656"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6651903.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51334..51828)
                     /gene="lspA"
                     /locus_tag="OO564_000657"
     CDS             complement(51334..51828)
                     /gene="lspA"
                     /locus_tag="OO564_000657"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6651904.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51828..54644)
                     /gene="ileS"
                     /locus_tag="OO564_000658"
     CDS             complement(51828..54644)
                     /gene="ileS"
                     /locus_tag="OO564_000658"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6651905.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54687..55628)
                     /gene="ribF"
                     /locus_tag="OO564_000659"
     CDS             complement(54687..55628)
                     /gene="ribF"
                     /locus_tag="OO564_000659"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6651906.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55636..55854)
                     /locus_tag="OO564_000660"
     CDS             complement(55636..55854)
                     /locus_tag="OO564_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6651907.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
BASE COUNT        13369 a        15359 c        14573 g        12706 t
ORIGIN      
        1 cctgaaacca tagcatccca atacccgcaa caatgccgat aaatacagcg ttaaaaagtt
       61 gcagcgtcat caacgccata cggctatgaa agaaaatcaa tccagtgtaa aacagcactc
      121 ctgccaccac tgcaatgacc atcattcgcc gcttaccata acgtttgaca tagtagccag
      181 ccagaatcat tgctggtatt tccagtccag ctgccgtccc catcaggaaa cccgccagtt
      241 tgtctggcaa tcctaaatcg ctactgatcc acaacggcat atcaataatg tacatagtgt
      301 tgcaggtcca cattaacgtc gaggcgacaa ataacatccg tacgttacta tcctgccagt
      361 caccttgcat tgataaagca ttttccgacg gcagttctac ccgcgccaca gacggaagca
      421 taaatgcaat caataccaga ctgagtgtga atatcccggc ggcaatcgaa aacatcactg
      481 taaagccgta attcaacgcc agcataaagg ccagcggtgg accgattacc catgccagag
      541 aaagctgcgc acgcatcacc gagctaaaca tcaccacttc tcgcgccgag ttatccgcgt
      601 attcccgcgc cagagcaaat aactgtggca ttgccgtatt ggccagagat gccagaagca
      661 caccacaggt gataagcgtc agataatgac gattaaatgc aaacagtagc gcattgccaa
      721 tagccatcaa acagcaaaat ataatcagtt ttcgccgatc gccctgactg tcagaacgtt
      781 ttgccaacca gaggcttacg ccgatcccag caatagcatt caccgtataa aagagtccga
      841 tccagaaagg ttgcgcgcca acctcacgac tcagaaataa gctcaatgta ggagcctgta
      901 gcgccccggc cacccccatc ataaaagcga ccagcataaa tgccgcgtaa acaccgttca
      961 tacggcgagc catcgtcatt atccagatca tacgttccct ttttagcgcg gcgagaataa
     1021 aaaaaaccag caggtataat ctgctggcgg gtgattttac accaatactc agtcacacat
     1081 gatgcaggca agtcaacttt cagaattgcg gtcatcccac tgcatcagtc cttcaagcat
     1141 ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac aacttcgctg tcgcggtaaa
     1201 atagtgctga taaaactgac gcatggggca tctccttgct tcatcgttag gaattattga
     1261 cttaatatag ggaaaataaa attgctgtct tttgcgcagg agttcccctt ttatgccatc
     1321 tgctcgtctg caacaacagt tcatccgcct gtggcaatgc tgcgagggta aatcgcagga
     1381 cacaacgctc aacgaactgg cagcgttatt gagctgctcg cgtcgtcata tgcgtaccct
     1441 gctcaacacc atgcaggatc gcggctggct gacgtgggaa gcggaagtcg ggcgcggtaa
     1501 acgctcgcgt ctgacattcc tctataccgg gctggcgctt cagcaacagc gggcggaaga
     1561 cctgctggag caggatcgta tcgaccaact agtgcagttg gttggcgaca aagcgactgt
     1621 gcggcaaatg ctggtttctc atctgggccg cagcttccgc caggggcggc acatcctgcg
     1681 cgtgctctac tatcgtccgt tgcgtaatct gctacctggc agcgcattgc gccgttccga
     1741 aacccatatt gcccggcaaa tcttcagttc gctaacgcgc ataaatgagg aaaatgggga
     1801 actggaagca gacatcgccc accactggca gcaaatatca ccgcttcact ggcgtttctt
     1861 tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg gaaatggacg acgtgatcgc
     1921 ctctttaaaa cgaatcaata cgctgccgct ctattcgcat attacggaca ttgtgtcgcc
     1981 gacgccctgg acgctggata tccatctcac gcagccggac cgctggttgc cgttactgct
     2041 ggggcaagtt ccggcgatga tcctgccgtg cgaatgggaa accctcagta actttgccag
     2101 ccatcctatt ggcaccggtc cgtatgcggt gattcgcaac agcaccaatc aactgaaaat
     2161 tcaggcattc gatgacttct tcggttaccg ggcattaatc gacgaagtta acgtctgggt
     2221 tctgccggaa attgccgacg agccagccgg tgatctgatg ctaaaaggtc cacagggcga
     2281 ggaaaaagag attgaaagcc gcctggagga aggttgctac tatttactgt tcgacagccg
     2341 cacccatcgc ggggcgaatc agcaagtcag ggactgggta agctatgtgc tttctccaac
     2401 taatctggtc tatttcgctg aggaacagta ccagcaactg tggttcccgg cttatggact
     2461 gctcccccgt tggcaccatg cccgcaccat aaagagcgaa aaaccggctg gcctggaaag
     2521 cctcaccctg accttttatc aggatcacag tgagcatcgg gtgattgccg ggatcatgca
     2581 gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa gagatcagct acgatcagtg
     2641 gcatgaagga gagatcgaga gcgatatctg gcttaatagc gccaacttta cgctgccgct
     2701 ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg ctccaacact gtattccgat
     2761 cgactggcaa gccgacgccg cccgctggcg caatggcgaa atgaacctgg cgaactggtg
     2821 ccagcaactg gtcgccagta aagcaatggt gccgcttatc caccactggc tgatcattca
     2881 gggacaacgc agtatgcgcg gcctgcgtat gaataccctc ggctggttcg attttaaatc
     2941 agcgtggttt gcgccaccgg atccatgatt gctggtagat aacaaaatca ctacactaac
     3001 gccgttctca acggggtgcc acgcaaacgc gtgcgctgag aaaatacccg tcgaacctga
     3061 tccggataac gccggcgaag ggatttgagg ctccttctca agtcctttgc cactcttttt
     3121 tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca gcgcccgttt
     3181 tcgctaaacc cgttctgact gtttatacct acgattcctt cgccgccgac tgggggcctg
     3241 gtccaaagat caaaaaagct tttgaagccg actgtaactg cgaactgaaa ctggtggcgc
     3301 tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga aggcaaaaat agtaaagccg
     3361 atgtggtgct ggggctggat aacaatctgt tagacgccgc cagtaaaacc ggactatttg
     3421 ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg cggctggaat aatgacactt
     3481 tcgtcccgtt tgattacggc tatttcgcct tcgtctatga caagaacaaa ctgaaaaacc
     3541 cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa ctggcgggtg atttatcagg
     3601 atccgcgtac cagcacgccg gggctgggtc tgctgttgtg gatgcaaaaa gtctatggcg
     3661 atgacgcccc acaagcctgg caaaaactgg cgaagaaaac cgtcacggtc accaaaggct
     3721 ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga tctggtactg agttacacca
     3781 cctctccggc ttatcacatt ctcgaagaga aaaaagataa ctacgccgcc gcgaacttta
     3841 gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac cgctgccagc aagcagccgg
     3901 agctggcgca aaaattcctc cagtttatgg tttctccggc ttttcagaat gcgatcccaa
     3961 ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc tgccgggttt gaacaattga
     4021 ccaaaccggc caccacgctg gagttcacac cagctgaagt ggcggcacaa cgtcaggcat
     4081 ggattagcga atggcaacgc gccgtcagcc gttaattccc ggctggttaa ttccaggtgt
     4141 aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg tttctcgccc tgtggtggaa
     4201 cgcgccgcag ggtaactggg tggcagtctg gcaggacagc tacctgtggc atgtggtgcg
     4261 cttctccttc tggcaggcgt ttctctcggc gctactctct gtcgtacccg cgatattcct
     4321 cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc gtctgtgcgc
     4381 aatgaccttg atcctcccgg tgctagttgc tgttttcggc attcttagcg tctatggtcg
     4441 tcagggctgg ctggcatcgc tctgccaatc gctcggtctg gagtggacct tttcgcccta
     4501 cggcctgcaa ggcattttgc tggcgcacgt attttttaat ctgccgatgg cgagccgctt
     4561 attactccag gcactggaaa acattcccgg cgaacaacgt cagcttgccg cccagcttgg
     4621 gatgcgcggc tggcattttt tccgcttcgt cgaatggccg tggctacggc gacaaatccc
     4681 gccggttgcc gcgcttatct ttatgctctg tttcgccagc ttcgccaccg tgctatcgct
     4741 gggcggcggt ccgcaggcaa ccactatcga gctggctatt tatcaggcgc tgagttacga
     4801 ctacgatcct gcccgcgcgg cgatgctggc gctgctccag atggtgtgtt gccttgggct
     4861 ggtgctgttg agtcagcgac taagtaaggc cattgcgccc ggcaccacgc tgctgcaagg
     4921 ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc gacacggtgt taatagtgct
     4981 ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc gtcgatgggg taaatcgcca
     5041 gttgccggaa gtgctggcac aaccggtgct gtggcaggcg ctgtggacct cgttgcgtat
     5101 tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg atgctgctat ggagcagtcg
     5161 cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg ctggagatga gcggcatgtt
     5221 gatcctcgcc atgccgggga ttgtgctggc taccggcttc tttttactgc tcaacaacac
     5281 tatcggcctg ccacaatctg ctgacggcat tgtgattttc accaatgcgt taatggcgat
     5341 cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat atcaccgccc gctacagtat
     5401 gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta aaagtggtcg agctgcgcgc
     5461 cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc gtgctgtcga ttggtgattt
     5521 tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc ctgccgtttt atctctacca
     5581 gcaaattggc tcctatcgca gccaggacgg cgcggtcacc gcgttaattc tgctactact
     5641 ctgttttctg ctgtttaccg tgattgaaaa actaccgggg cgaaatgtta aaactgactg
     5701 atatcacctg gctttatcac catttgccga tgcgttttag cttaacggta gaacgcggcg
     5761 agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag taccctgctg aatttgattg
     5821 ccggttttct gacgccagcc agcggttcgc tgactatcga tggcgttgat cacaccacga
     5881 cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga gaacaacctg ttcagccacc
     5941 tgacggtcgc gcagaacatc gggctggggc taaatccggg gttgaaactg aacgcggcac
     6001 agcaggagaa aatgcacgcg attgcccgtc agatggggat tgataattta atggcgcggt
     6061 taccgggcga gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt tgtctggtgc
     6121 gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg gcgttacgtc
     6181 aggagatgct gacgctggtg agcacgagct gccagcagca aaaaatgacg ctattgatgg
     6241 tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg ctcggtggtg gtcgccgacg
     6301 ggcgcatcgc ctggcagggt aagactgaag aattgttgag cggtaaggcg agtgcttcgg
     6361 caatattggg gattacgggt tagtatgcac tgtcagatgc ggcgtgaaca ccttatccga
     6421 cctacggaca gtgcgttttg taggcctgat aagacgcggt cagcgtcgca tgaggcaacc
     6481 cgcacaagac aatcccctaa accccaacca ctttacgcaa aatatcgata tacaccggca
     6541 tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa atcaacggcg
     6601 tcagccacaa caaacggccg cgcgacaaat aatgacttaa acgatcggtt gctttaccgc
     6661 tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc gtcgccagca
     6721 gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc gcgcccgcca
     6781 gaatcccagg caggaagtaa aacggtggcc acagcaggca gccgataata ttcggcgtaa
     6841 tgaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc ggacgcgtcg
     6901 ggccaacaaa gcgaccgacc agaatggtga acatgctgtg ttgatgcaac gcatgttcag
     6961 ttttatcgag taatgctttg tttttcttca gaaatgacca gcgatgcaac ggctttttaa
     7021 aacgccagcc cagccagaag gagatccagt cgcccagcag acaaccgaca atccctgcca
     7081 gccaagcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc cccgccatca
     7141 gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag gccaccaaca
     7201 ccaccgccat caatgaatac acggtagatt gggtaataaa gtgttccagc aatgcttgca
     7261 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
     7321 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
     7381 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
     7441 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
     7501 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
     7561 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
     7621 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
     7681 gcctcgcgta cacgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
     7741 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
     7801 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat acggctggtg cgcttcatcc
     7861 gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
     7921 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacaaccg
     7981 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca gacaaattct
     8041 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
     8101 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
     8161 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
     8221 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     8281 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
     8341 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     8401 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     8461 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
     8521 tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga ttgcaattgg
     8581 cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca gcggtgaaga
     8641 gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt gtgatgcccc
     8701 gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag cggcactgaa
     8761 aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga ttggcgttga
     8821 cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg cgctgcgccc
     8881 ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg aaagaccaca ctgcggttga
     8941 agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact actcccgcta
     9001 tattggcggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg tgactcgcca
     9061 ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact gggtgccagc
     9121 tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca gcgccgggca
     9181 taaatctctg tggcacgaaa gctggggcgg cttgccgcca gccagtttct ttgatgagct
     9241 ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgacacct ggactgccga
     9301 tattccggtg ggcaccttat gcccggaatg ggcgcagttg gcgcaggcgc acagcctaac
     9361 gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc
     9421 gttggcgagc gggcagttaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga
     9481 tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc
     9541 gtactcggct ggccgctgga acagcttgcc gcccagcatg cggaactgaa agcgcaaatc
     9601 aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct
     9661 ctggatcacc tgccggtggt gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac
     9721 caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacactcc gctgctgttc
     9781 ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat
     9841 caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc
     9901 attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag
     9961 tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc
    10021 ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa
    10081 caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa
    10141 caacactatc ttccaacttc cgccccggca caggctgccc aggccgttgc gactctataa
    10201 ggacacgata atgacgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca
    10261 tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc
    10321 gctgaatacg gaagcgaaac tgccctgcaa actggtgttg aaaccgctgg gcaccacgcc
    10381 ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt
    10441 ggtgtggctg cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa
    10501 caaaccgttg ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagtatcga
    10561 tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg
    10621 cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac tggcaggata aacaagccca
    10681 tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa
    10741 agtctgccga tttggcgata acatgcgtga agtggcggtc accgatggcg ataaagttgc
    10801 cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt
    10861 ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta
    10921 caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc
    10981 gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac
    11041 cacctttgaa gatttgcacg gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat
    11101 gcagcagggt tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat
    11161 catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta
    11221 tcacttcgag aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc
    11281 gattgccgta gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga
    11341 cgatcctgcc cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat
    11401 tgatctcggc gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca
    11461 ctccctgccg aaactgccgg tggctaatgc actgtggaaa gcgcaaccgg atctgccaac
    11521 tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct
    11581 gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga
    11641 taacgacacc cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg
    11701 atttcgtcgc taagtagcca catcaggtat gtaacgcctg atgcgacgct gactcgtctt
    11761 atctggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattcaac
    11821 gcctgatgcg acgctggcgc gtcttatcag gcctacgcgc tgcgattttg tagaccggat
    11881 aagcgaagcg catccggcac gaaggagtca acatgttaga agatctcaaa cgccaggtat
    11941 tagaagccaa cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca
    12001 gcgccgttga tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg
    12061 tcatgaccgc tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta
    12121 cgaaaaagcc ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca
    12181 ttggcggcat tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt
    12241 cgattccagc aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc
    12301 gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg
    12361 tagaaacctt tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt
    12421 cccacggccc gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc
    12481 tggaagaggt cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata
    12541 tgcagcaaac gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg
    12601 ggcagtaatg actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct
    12661 ggttttttga tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg
    12721 ggacaccccg caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca
    12781 ggttacgctt gcaccgcaag agtccgtggc gtttattcac gccgatcagg ttccccgcgc
    12841 tcagcatatt ttgcagggtg aacaaggctt tcgtctgaca ccgctggcgt taaaggattt
    12901 tcaccgccag ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga
    12961 aaagcgcctg cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg
    13021 ctatctgatg gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg
    13081 cactatcgtt aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt
    13141 ttctatagat attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg
    13201 cgggcagcgc atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt
    13261 cgaactggaa tacgtcgcca gccgcccgct gctgctggaa aaactcaacg cctggtttgc
    13321 aaaccacaat cccgatgtga tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct
    13381 gcaaaaacat gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct
    13441 ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct
    13501 aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct
    13561 ggaaactgtc gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggttcg
    13621 aatggacgaa attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct
    13681 gaaagattgc gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact
    13741 cgaacgagca acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt
    13801 tggtcatctc tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga
    13861 agtgccgccg cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga
    13921 ttcagtgctg gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat
    13981 tgatcccgtc gggctggttg aaggcatggc gcaacctgat ccagagcaca gtaccgaagg
    14041 ttttctcgat gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat
    14101 ctggcacggg cgcgatgaag ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa
    14161 gatcattatg aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc
    14221 gcgcctggca tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt
    14281 gattgaagcg cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct
    14341 aaaaggtgca cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt
    14401 taacgcctgg tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga
    14461 gtatgaaact catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag
    14521 taaaaagcgc tacgccgggc tgattcagga gggcgacaag cagcggatgg tgtttaaagg
    14581 gctggaaacc gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctgta
    14641 cctgcgtatc ttccgcaacg agccatatca ggagtacgtc cgccaaacca tcgacaaact
    14701 gatggcgggt gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag
    14761 cgaatatcag cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa
    14821 tcaaaagcgc ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac
    14881 caccaacggc ccggagccgc tggactacca acgttcacca ctggattacg aacactatct
    14941 gacccgccag ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc
    15001 tacacttatg accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca
    15061 ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac
    15121 ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga
    15181 tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa
    15241 gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc
    15301 catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt
    15361 tcaaccctgg tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa
    15421 aagaagaaaa tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg
    15481 tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc
    15541 tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt
    15601 attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga
    15661 tcccgcatca gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc
    15721 tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac
    15781 tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt
    15841 ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt
    15901 atgccgaagc tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct
    15961 cgctggattt tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg
    16021 acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg
    16081 aatatcaggc cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga
    16141 ccccggaaca gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc
    16201 gtttccacga ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg
    16261 ccgttgccat tctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg
    16321 agatgatcgg cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag
    16381 atgcccagag cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc
    16441 gcgtgctgtt ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca
    16501 ccattaagct gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg
    16561 gcgcacgtaa aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc
    16621 aggaatttga aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga
    16681 tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca
    16741 ctgcgctgca actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc
    16801 acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca
    16861 ccggcgcaca ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg
    16921 ccagccacat ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg
    16981 gtcgtctgga tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga
    17041 aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca
    17101 cctgcccgac cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg
    17161 cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg
    17221 aagcgctgaa agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg
    17281 gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc
    17341 tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg
    17401 acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt
    17461 cggaagatgg catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac
    17521 agtttattac ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg
    17581 ataccggtag cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt
    17641 tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct
    17701 tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc
    17761 aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca
    17821 aactggttaa caccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga
    17881 tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt
    17941 ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg
    18001 aactgaccgc cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt
    18061 ggcgtctgga tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg
    18121 gatggaaaac tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca
    18181 tattatggtg gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca
    18241 caaagacagc gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg
    18301 tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga
    18361 gttaaaacgc cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg
    18421 gcatccatcc cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg
    18481 cccgaaacag aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt
    18541 ggagtatgcg gcggataaca cggcaagagc ggtgttaaaa ccgattaccg ggcgttcgca
    18601 tcaattgcgt gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc
    18661 atcaccagaa gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat
    18721 tacccatccg gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc
    18781 acgctggcgc agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc
    18841 gattgcctga tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa
    18901 ccctttctgc tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc
    18961 catctccatc atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact
    19021 catcagctta cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag
    19081 cacattacag gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc
    19141 agattgctgc tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg
    19201 gtcaaactgg gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc
    19261 attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg
    19321 aattaagtca aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg
    19381 gtaattatct gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg
    19441 aaggttcatt cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc
    19501 tttggatttg gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg
    19561 ctcacgctgg ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc
    19621 aattaacagg cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac
    19681 gccaatgatt tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc
    19741 tatctcccac aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat
    19801 cgcctataat ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct
    19861 attcggacag gattaacact agcgtagaga tgacaagtac gttagtctct gaccgtttgt
    19921 cacgcgcaac gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca
    19981 ctctcctggc caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc
    20041 tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata
    20101 ccaatgactt acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg
    20161 ccgtgtttac tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag
    20221 tgcagctcca tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc
    20281 tcggtaatgt ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga
    20341 atctgaacac caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg
    20401 gtcgcggtaa agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata
    20461 acggtagctt tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa
    20521 ttattcatga ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc
    20581 cggtaccgat cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg
    20641 gtttcttgat cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat
    20701 attactggaa catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc
    20761 gtggcaacat catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga
    20821 tggaactgga ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca
    20881 gttcacgtcg ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt
    20941 tcaacgtcga ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt
    21001 acggttccag tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa
    21061 acttcaatgc caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta
    21121 gctactcggc agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg
    21181 atacgcgtat ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag
    21241 caacccgtgt tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca
    21301 tcaataccga agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata
    21361 actccagaaa cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag
    21421 ttgacggcaa aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa
    21481 cgctggaacc gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata
    21541 actacgactc gtctctgctg caatctgact actctggcct gttccgggac cggacttacg
    21601 gcggtcttga ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat
    21661 atgatgatgc tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg
    21721 agtctcgcac tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg
    21781 tgtgggcagg cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc
    21841 agtacgatac acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg
    21901 atgaagaccg tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta
    21961 cgctgcctaa gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg
    22021 ctgtcgccag caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca
    22081 ccaatgcgaa caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg
    22141 caattcgcgt cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg
    22201 tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc
    22261 tgggtacgca agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg
    22321 ttgatttacc acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg
    22381 aaaacgctgc ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta
    22441 gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga
    22501 ttaatgcagt cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg
    22561 acgctgcgcc accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg
    22621 cagaaaatgg gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg
    22681 aaacagaaca acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac
    22741 tacaacactt atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac
    22801 gaggtgcgtc gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg
    22861 ggtaaccaaa acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg
    22921 gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat
    22981 caggcgcgta acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag
    23041 gcgctgaacg gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc
    23101 caggcattaa gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc
    23161 ttccatattc tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa
    23221 gttcatgctc gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc
    23281 gtgaaactgg agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca
    23341 gcgaaagagt tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct
    23401 acaccagata ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa
    23461 atgagtgcac cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt
    23521 aatgtcgata aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt
    23581 aagttctcgg aagaagcagc aagctggatg caggaacaac gtgccaacgc ctacgttaaa
    23641 atcctgagca actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg
    23701 ggattggccc ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg
    23761 tttgtgccga tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc
    23821 tccgccctta ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac
    23881 ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt
    23941 acgtggtgga gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc
    24001 tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc
    24061 ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg
    24121 aagaacttcg cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta
    24181 tcacccctgc acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat
    24241 ttggtattgc cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg
    24301 gtcatatggg tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc
    24361 agggaatgaa actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg
    24421 ataacgccga cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc
    24481 agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg
    24541 accacggcac cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta
    24601 cggcgcttaa tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca
    24661 gggccactta gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga
    24721 cagcattgtt tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg
    24781 tcttgcggca ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact
    24841 tgaccgcgat ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat
    24901 ttatcagcag gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc
    24961 gctgcgtgtt ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt
    25021 tagctatact gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg
    25081 tctggttgca ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta
    25141 ttgcaacgtg atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt
    25201 ggattccgcc gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatgt
    25261 tcgcgtgttg agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa
    25321 cagcctcggc aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat
    25381 gcgagcggaa aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa
    25441 cgcgcctttg caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa
    25501 agcgtctaca ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc
    25561 gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc
    25621 accaatggca atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca
    25681 cttatcgcgc ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg
    25741 ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac
    25801 attcccgtat tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac
    25861 cttattggcg acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa
    25921 tttacccccg ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt
    25981 tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc
    26041 aatcacgatc tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat
    26101 cgcctgacac cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc
    26161 cagcctctgc tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg
    26221 ccgcagtggg atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg
    26281 agtgactcct atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca
    26341 ccggaattgc gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt
    26401 ttttgcttcc cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct
    26461 gccccactga aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc
    26521 tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat
    26581 accggctgct gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt
    26641 gtccagccgt cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct
    26701 gatacaggaa ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct
    26761 atacaaactt accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg
    26821 gcgtcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg
    26881 gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg
    26941 aactgctcat aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc
    27001 gcaatggctt catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg
    27061 ctgaggataa tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg
    27121 cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg
    27181 ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga
    27241 ctgatcattg agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt
    27301 cgtcgactgg tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc
    27361 tctccccctg gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt
    27421 gatttacgtc actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc
    27481 ggtatgttta ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc
    27541 gcggtcctcg gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg
    27601 ggctttggtg tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg
    27661 gaacacatcg gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag
    27721 acgcccggtt ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg
    27781 acgcaaacga atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa
    27841 atgttctttc accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat
    27901 cagcacttcc gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc
    27961 ataaaacact ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag
    28021 caccaccatt ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc
    28081 gaatccggcg ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc
    28141 ctccgtagaa gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat
    28201 cgacagcgcc accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat
    28261 ctgcgccgca ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa
    28321 ccagcgacgc tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat
    28381 tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt
    28441 gccaaagtct atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa
    28501 cggttcgata tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc
    28561 cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa
    28621 cacaaggaat aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg
    28681 cagcgccgga cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac
    28741 ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag
    28801 cgggatcatc gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc
    28861 actgcggccc atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc
    28921 cgttgatgag agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag
    28981 taacatacag aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc
    29041 gaacactgcc gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa
    29101 cagcatcagc accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag
    29161 tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag
    29221 accaagacgt accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg
    29281 aaccagcgta tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt
    29341 ggcgcgcctg cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg
    29401 gtggcagcca gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat
    29461 caaagcccgg atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc
    29521 acagcaaatg tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca
    29581 aaactttatc gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct
    29641 gccagacgat cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga
    29701 agtcaggata gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt
    29761 caagcatccg tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga
    29821 tcatagttat tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt
    29881 cacgcggctt atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc
    29941 cattctctgc ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc
    30001 cggggcaaac gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat
    30061 cgtgttactg ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt
    30121 gccaatacga cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa
    30181 gagttcatta ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac
    30241 cgcaaaggcc atcactacca gccagatccc catatccggg attagcccca aaaccgccag
    30301 cgccagcgtc atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag
    30361 ccacagcatc ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag
    30421 tgccgcgttt ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta
    30481 aatggcgaac attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg
    30541 aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa
    30601 agccaccggt tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt
    30661 gcgtaataac cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca
    30721 agggatcgcc gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta
    30781 gccgaccaga tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga
    30841 aaacgcccgc tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc
    30901 tgcaccgatg acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac
    30961 acacatcgtc gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg
    31021 ccgtccgact ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag
    31081 cgttcccgcg cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg
    31141 ctccagcgcc acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc
    31201 ccacagcaaa atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg
    31261 ttgcatgggt tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc
    31321 aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca
    31381 gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga
    31441 agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca
    31501 gctccagcac ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga
    31561 cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt
    31621 cactcctttc atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag
    31681 gattttcttg cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac
    31741 gcccaccgcc agttccgggt agccgctaaa catgcgtggg ttatcaagga acgccggtag
    31801 tttctggtac atgcgcatat cgcgcagcgg gctgctctcc agatgctgac gatattccgc
    31861 gagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc
    31921 ttccccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat
    31981 tccggcggca tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat
    32041 gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg
    32101 tgcaacggcc ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc
    32161 atgcagatga tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag
    32221 gaagccgccg cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg
    32281 attaccctgc aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt
    32341 cacgccaacc gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag
    32401 gatggagttc accccatcag caaggagcac cgttttcgct tcaatcacat cgccatcggc
    32461 ttctacaccg acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat
    32521 taactgcgcg cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa
    32581 aacggagtaa gaacgctggg atagcgaggt ttcgtcaccg ttgcagtagt ccatagtcat
    32641 cgctgacttt tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc
    32701 ggaatcggcg aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt
    32761 aacgttcttg gcacccgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg
    32821 ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa
    32881 gatatcttcg gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga
    32941 gtggatcaac gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca
    33001 atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg
    33061 gtttgcgacg cgttagcgcc aaccatgtgc tggatctgcc cggagatccc caccgccagg
    33121 tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat
    33181 ttttcgtttt ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc
    33241 tgttctgcca gtgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga
    33301 cgggctttgt cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc
    33361 gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc
    33421 tgagccgcat cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt
    33481 tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt
    33541 acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc
    33601 gccagtaatt tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg
    33661 cgaatagtgt cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg
    33721 tttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg
    33781 aggacaaagg cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga
    33841 cgagaggggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta
    33901 attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt
    33961 caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg
    34021 cgttcacggc gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg
    34081 cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa
    34141 caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg
    34201 tgagggtatc tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga
    34261 ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac
    34321 agaggatcag atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt
    34381 gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg
    34441 atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg
    34501 tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat
    34561 cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga
    34621 ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc
    34681 cagaaatcat gaaggaaacg tcgttcatat cggtagtgtt aattacagat atcacgccct
    34741 ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca
    34801 actttcaata ttgttaagtt cctcaccaat attgaaaaca tggcgtagca aaaagaaatt
    34861 ttcaatattg ttttatagat caccaatatt gaaagtcaga tgctattttt tacccacaaa
    34921 agaacataac aaactgatta ataaccaaaa ttaacgaaaa acgtcctcta ttcactcgaa
    34981 acaaaaatgt gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca
    35041 ttaacgcgtc cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg
    35101 ggaacacaaa ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga
    35161 aaaacgggaa tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt
    35221 tggcttacag tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac
    35281 gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg
    35341 ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt
    35401 agcaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc
    35461 tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac
    35521 tcgacaggag cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg
    35581 tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg
    35641 gaagtgattc gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg
    35701 ttgttcggta ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc
    35761 agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tgacattccg
    35821 cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc
    35881 gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc
    35941 ttcctgatgc tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc
    36001 accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc
    36061 atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt
    36121 tacgccatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa
    36181 ctgtgcttcg gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc
    36241 ggtagtaaca ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa
    36301 cagtacggtg tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc
    36361 accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc
    36421 tcttataccc tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg
    36481 ctggtgcgta tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc
    36541 ggcggcctga aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc
    36601 aacattatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta
    36661 acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg
    36721 tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag
    36781 tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc
    36841 gacagtctgc tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc
    36901 gccgtgtgga tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg
    36961 ggcgggttca ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct
    37021 ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc
    37081 gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt
    37141 agtaagtgtt ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac
    37201 ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc
    37261 atcaaagtga ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc
    37321 tttgacgatg tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac
    37381 cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg
    37441 gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag
    37501 gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac
    37561 tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct
    37621 ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc
    37681 gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg
    37741 cgtgacctgc gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg
    37801 ggtcgtgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg
    37861 cgtcttatca ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc
    37921 ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga
    37981 aattcggacc gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt
    38041 ttgccggaca aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct
    38101 gggccgacac cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg
    38161 cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa
    38221 ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg
    38281 atgaagtact gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc
    38341 agtacggcac cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca
    38401 gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg
    38461 ccgattactt ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc
    38521 gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg
    38581 gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag
    38641 gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga
    38701 tggaactggt gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc
    38761 tactcggtac gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt
    38821 acggcccact ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag
    38881 ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac
    38941 tggaaagcaa tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg
    39001 gtcgcacctg caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct
    39061 ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca
    39121 gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc
    39181 gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat
    39241 agaggtgcaa tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg
    39301 gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg
    39361 tatagttatc ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg
    39421 gggattcgca aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt
    39481 tgctggttcg ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta
    39541 cgcgaggaaa gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg
    39601 caattttatc ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt
    39661 tgcctgacag atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa
    39721 aatcaacaac ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa
    39781 attctcttca cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac
    39841 ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac
    39901 atgacggtaa tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt
    39961 tctgccgggg ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag
    40021 gcgcagaagt accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg
    40081 gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc
    40141 aacttgtcgg agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg
    40201 tcttatggga tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga
    40261 cgctggccgt cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac
    40321 aatcgcccgc tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa
    40381 accatcttca aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac
    40441 ggctggctac ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc
    40501 gatcgccgct gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa
    40561 aatatcatcg ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg
    40621 attcgcgatg aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa
    40681 gaggaatttt ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg
    40741 gagatcagaa aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa
    40801 taaatgaaac gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca
    40861 ttttttgcag ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa
    40921 tcattacatc tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg
    40981 aatgctattg atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac
    41041 gatccgcaat tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc
    41101 tgggatttaa aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt
    41161 tttgcgggat taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc
    41221 tacgcctttg gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat
    41281 aacgccagct tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg
    41341 ctgcgtctgc cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga
    41401 cgaatgggcg cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg
    41461 gaactgatgg ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg
    41521 attgcggcgc tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat
    41581 cgctatattc gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc
    41641 attgaagggc cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa
    41701 ccgtgagcta ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc
    41761 atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac
    41821 tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg
    41881 atggctgcat tatgcatggc tactgcgaca ctgacaccat cgttggagaa aacggccata
    41941 tcgggcatgg agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga
    42001 acagcgtaat tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct
    42061 ttgtcaaagc aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg
    42121 ctgtacgcag tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc
    42181 aggatcttgt cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg
    42241 aggaaaatcg cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc
    42301 ggatgcgacc tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc
    42361 ggtatcaacg agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg
    42421 ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac
    42481 taatttttcc cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac
    42541 tttaaccagt cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac
    42601 ttcgcagctt atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct
    42661 gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat
    42721 catccattcg gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac
    42781 tccagtttca caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaagacca
    42841 tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa
    42901 tcctgcttat gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa
    42961 aacggtcctc atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc
    43021 cgccacgaca cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc
    43081 agtcgcatcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata
    43141 atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg
    43201 gcctgaggtg gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg
    43261 cggacggcct tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac
    43321 aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag
    43381 gtccaccacg cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt
    43441 catggtggag ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac
    43501 gcccatgact tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa
    43561 tttattgaac ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt
    43621 tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg
    43681 tacgccggtg gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat
    43741 caggtaaact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa
    43801 ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt
    43861 gtacgctggc agcgaacatg cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc
    43921 catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac
    43981 tgcgtcatca aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt
    44041 ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag
    44101 aacgtaagac ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat
    44161 ttcaatagca gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg
    44221 ctggaagcgt tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg
    44281 tacgccagcg gcttccagtg cacgcgccag tttcagcggg gtttgaccgc cgtactgaac
    44341 gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg
    44401 ctcgaagtag aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt
    44461 aaccataatg gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta
    44521 gtcgaattcg ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc
    44581 acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt
    44641 gtcggtggca aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata
    44701 ttggtcacgc agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc
    44761 atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc
    44821 cacttccgcc actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg
    44881 gtcaatgtta gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc
    44941 gatgtaccag atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc
    45001 ttccgggtca tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg
    45061 cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga
    45121 tttcatctga gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg
    45181 aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt
    45241 gatgtcgttc atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc
    45301 aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat
    45361 ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc
    45421 ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc
    45481 tttgtcagtc agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat
    45541 cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac
    45601 ttccatctcg tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg
    45661 agagagatcc agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc
    45721 gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc
    45781 ggcaacagcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc
    45841 aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc
    45901 agtggcacca atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag
    45961 cgcgcagttc agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt
    46021 ttcaataatc ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat
    46081 ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc
    46141 ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat
    46201 aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc
    46261 tcttttactc ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg
    46321 tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg
    46381 aacgccggtt tatcgatacg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc
    46441 gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg
    46501 gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca
    46561 aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag
    46621 atgccgaata ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg
    46681 cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc
    46741 acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc
    46801 atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg
    46861 tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta
    46921 taggcttctg cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg
    46981 cgggcttttt ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc
    47041 tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc
    47101 acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca
    47161 atcagcggca ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg
    47221 gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa
    47281 taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc
    47341 ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga
    47401 accaatagcg ctgacttaat caaaacacct ccatagaata ttcactcact ttatttgcat
    47461 attaattcac agtgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa
    47521 acggcggcat tctggagata tgggcgctaa aagtcaactt aatggcagga ttttttatat
    47581 tattttgtgg ctcatgcatg ttttacaggc attaagcgac aaaaacatca tctaacctgt
    47641 tctggaaaac gcttgcgcag caagaagatt gcatgaaata aaaacgagca ggaaaaagtg
    47701 gaccaaatgg tcaaaattta cattaaaaca acaaaaatag aagacgaaac atgtaatttt
    47761 tggcagttat agaaaattta aaagcataac accaaaaata aaagggctaa aaattaaagc
    47821 cctttataat taataaatta aatgtgttac ttttgcatca taataaacat tttgtggtta
    47881 caaattattg agatcaagca catctcgcat atcaaaaaga ccactttcct taccactcaa
    47941 ccacaaagcc gatcttaccg caccgttagc aaatgtcatg cggctggacg ccttatgggt
    48001 gatctccaga cgctcgccaa tatcggcaaa catcgcggta tgttcgccaa cgatgtcacc
    48061 tgcacgcacg gtggcaaaac caatggtacc aggcacacgt tcaccggtat ggccttcacg
    48121 actgtagacc gcgcaatctt tcagatcttt atcaagggcg tgggcgatcg cctctcccat
    48181 tgccagtgcg gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc
    48241 gatatcggtg tagtcaccca tcactttggc tgctttctcc agcagcttaa gcatgacgtt
    48301 aacgccaacg ctaaaattgg cggcaaagac aatcgcaata tcggcggcgg cgtcacgaat
    48361 tgcctgttta ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg
    48421 ccgacaaaaa gcgagatggt tcagcgtacc ttccggacgg gtaaaatcga taaacacatc
    48481 aaaatcatct tttatcgcat caagactgct ttgcacggta acgcctgttt tccctgcacc
    48541 agccagctca ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc
    48601 cagctgcacg ccttctaatg ccagtgccgc ctgaatcaac tggcggccca tacgcccccc
    48661 ggctcccgcg atggcaacgc ggatgtttgc atcatgcata gctattctct tttgttaatt
    48721 tgcatagacc gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat
    48781 aattagaaat taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga
    48841 ctgcatgccg ataacatgtg acaggttacg gcaccagagc cagcacctca gccacccact
    48901 gttggaagcc ttttacattc agatccaatg ccacctgtac attggctggt ttgcccaggc
    48961 aaccgtcgat atcaaccacc gtcgtgcctg aggtaaattc gccttgagtt tctaccgcga
    49021 caaaacacgg cttgagggtg aataagtccg ggcgcaccag ccaggcgatg gcgcagagat
    49081 cgtgcattcg cagaccgctt tgcatactgc cgctacggta gtggctaaac agggcgtgga
    49141 gcatttttcc ggtgcgattc aactccggta aagtagcgag atagtcaggg gttaatattg
    49201 cctgattggt gacatccaga ccgcacatga caatttcgat gccgctacgg aagacacagg
    49261 cagccgcttc tggatcggca gcgatattaa actcggcatt tggtgtacag ttgccgcgac
    49321 cagcagaacc gcccatgatc accagacgac gaatatacgg tttacattcc gggcattgta
    49381 aaagtaacag cgcaatattg gttaacgggc cgatggccac cagggtaacg ggctctggtg
    49441 cacgcatcag ggcatcccga atcgccagaa acgccggtat cccgagtggc tttcggttgt
    49501 gctcaacaaa gtcgtagcca gccattcccg attcgccgtg cacagatgct gcatcacgcg
    49561 gtgcgcgtac cagcggcaca gcggctccct gggcgagcgg aatctccgca ttccagaaat
    49621 gcagcagttg cagggcattg cgggtagttt tctcaaccga gacattaccc gcgacggtgg
    49681 tcatcagttg caggtcgagt tcgggggcaa aaatcgcggc ggcaatggcg acggcatcgt
    49741 caatgccggg gtcagtatcg aggaagatag gtaaacgcat gttttcttca taaaaaatgc
    49801 cggtaacaag accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg
    49861 aatatcgaca cgcagctctt tcggcacttc gaaaacaata ttttcttcac ggccttccag
    49921 cggaatggct tcaccaccac ccaactgctg caaacgtgcc accacattct gcaccagaat
    49981 atccggagcc gatgcgcccg cagtcacgcc gacgcattta acctctttca cccactcttc
    50041 ctggatatct ttcgcatcgt caatcaaaaa cgcgtgtttg cccatacgct gggccagctc
    50101 cgccagacgg ttggagttgg aggagttttt cgaaccgacc accaacacaa cttccgcctg
    50161 ttctgccagg gcgcgtaccg cttcctgacg gttagtcgtg gcgtagcaga tgtcatcttt
    50221 gcgcggaccg acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtatc
    50281 atccaccgac agcgtggttt gggtcataaa ggagagcttc tcttcgtttt tgaccgtcag
    50341 tttccacaca tcgtctggcg attcgaccag atacattccc ccttcggggt tgctgtactg
    50401 ccccatcgtc ccttccactt ccgggtgccc ggcgtggccg atgagaatag attcttcgcc
    50461 acggcgactg gcgcgggcaa cttccatatg cactttggtc accagcggac aggtggcatc
    50521 aaacaccgtc aaatcgcggc ttttcgcttc gttacgaacc gcctgagaaa caccgtgtgc
    50581 ggagaaaatc aggatcgcgc cgtccggcac ttcgctaatc tgctcaataa agatagcccc
    50641 acgctcgcgc aagctatcga ccacgtagcg gttatgcacc acttcgtgac ggacatatat
    50701 cggtgcgccg taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc
    50761 acaaaaacca cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga
    50821 tttccagcac ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa
    50881 cggtaatgga gtcgccgtta atttcgcgga tcacaccagg catctcactg ccatccattg
    50941 cggtaaaaag catgattgcg ccaatttctg gctcgcctgc atccataaat tcacggcggg
    51001 agaagtactg aatcaggtcc ggtgacggca cgccaaacgc cgcatctggc tccagcgaga
    51061 aggtggtttt gtcgcccact ttcagcccca gcaggtgttg ctccagccct tcagaaagag
    51121 aagcatcgcc caggcggaac agcgccggtt taccgttgtt gcgagtagac tcggcggtgg
    51181 tgccatcgtc gagttttagc gtgaagtgca ccaggacggc gctattgctc tgtacagatt
    51241 cagacatgaa ggtttgctct tatttttgag gatttttgcc ggatgcggcc tacgatttcg
    51301 tagcaatctg taggccgcat ccggcagggt ttattattgt tttttcgctt tggaaggtaa
    51361 aaacccttcc agcacgatca gtgccgcacc gacacaaatg gcagtatcgg caaggttgaa
    51421 ggtggcgaag tgccagtcgc cgacgtagaa gtcgatcata tcgacaacga agccgtgcca
    51481 caggcggtcg aacaggttgc ccagcgcgcc gccaataatc agcgcgtaag cgatattgtt
    51541 tagcttctgc gtggccttcg agcgatacat catcactgcc aggagcacgc taataccaat
    51601 cgcaataccg gcaaagaacc aacgctgcca gccgccgcta tcggcaagga aactaaacgc
    51661 cgcgccatag ttacgcgcat aatgcagatt aagcgacggg aacagcggga ccgtatcccc
    51721 cagagcaaag ttctggagga tcaggtattt gctgcccaga tcgataatca gcacgactac
    51781 caccagccac agccagcgta gccctgttga acagatcgat tgactcatca ggcaaactta
    51841 cgtttttcac cgtcaccggc gacgttgctg acacagcggc cgcagatttc tgcgtgttcc
    51901 gccaccttgc cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc accttcggct
    51961 ttactcaacg cgactttcag ccctttgagt acttcgctct gctgagcatc agcaggtgcg
    52021 tcgttatagt ctgcaacggt agcgccggag gtcaacagga caaatcgtaa ttcatcgccc
    52081 agcgcggtca gtttcgccgc cagttccggt tctgcataca aggttactgc cgcttccagc
    52141 gagccaccca ctttcttgtc ggcacgcgct tgctcaatga ctttgttcac ttcgccacgc
    52201 actttcaaca gctcgtccca gaacgcatcg ttcattgctt cactgtctgc cagaccaaac
    52261 aggccttcgt accactcacc ggtgaagacg tatttttcac gttcgcccgg cagatagccc
    52321 cacacttcat cagcggtgaa ggagaggatt ggtgccatcc agcgcaccag cgcttcagcg
    52381 atgtgataca gcgcagtctg gcagctacga cgcgccacgc tatccgcttt ggcggtgtac
    52441 tgacggtctt tgatgatgtc gaggtagaag gaacccatct caacggagca gaagcgcatc
    52501 agacgctgta ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat gtcttcctgt
    52561 gccgctttcg cacaacctac ggcccagcga tccagtacca ccatctcttc cggtttcacc
    52621 atatcttttg ctggatcaaa accattcagg tttgccagca ggaagcgcgc ggtgttacgg
    52681 atacgacgat agctatcggc agcacgtttc aggatctcgt cagaaacggc catttcaccg
    52741 gtgtagtcgg ttgatgccac ccacagacgc agaatatccg cgcccagttt gttcatcaca
    52801 tcctgcggcg aaacggtgtt gccgatggat ttagacatct tgcgaccctg accatccacg
    52861 gtaaagccgt gggtcagtac ctgacgatac ggcgctttgc ctttcatcgc ggtggagatc
    52921 attagggaag acatgaacca accacggtgc tggtcagaac cttccagata catgtccgct
    52981 gcgtgaccgg caaattccgg acgcacgtca acaacagaag agtgggtaga tccggagtca
    53041 aaccatacgt cgagagtatc cggcactttc acatactggt cagcttcgtc gccgaggatc
    53101 tctttcgcat cgagatccca ccacgcctgg atgccgtcaa cttcaacgcg tttagccact
    53161 tcttccatca gttcgagggt acgcggatgc agctcttccg tgtctttgtg cacgaacagt
    53221 gacatcggta cgccccaggt gcgctgacgg gagatacacc agtcaggacg gttagcaacc
    53281 atcgactcga tacgcgcctg gccccagtcc gggatccact gcacgccttt gatctctttc
    53341 agtgactgcg cacgcagacc tttctgatcc atgctgacga accactgcgg tgtcgcgcgg
    53401 aagatgatcg gcgttttgtg acgccagcag cacggatagc tgtgctgcat tttctcaacg
    53461 tgcagcagcg cgcctttttc ctgcagcagc gcaacgacga tgtcgttcgc tttgaagacg
    53521 ttcacgccat ccagcgtcgg ataagtgccc ggcagataag tgccgtccgg gccaaccggg
    53581 ttagcggttt ccaggccgta tttctgaccg atcacatagt cgtccgggcc gtggccaggc
    53641 gcggtgtgaa cggcaccggt accggcatcc agggtaacgt gatcgccgag gattgccgga
    53701 acgtcgaagc ccataaacgg atgggtaaag cgcagcagct caagctctgc accttttacc
    53761 gtgccgagaa tggtgtaatc ggtcacgccg atacgctgca ttacgctttc aaccagatct
    53821 ttcgccagaa tcgcggcctg accgtcgatc tgcaccagcg catagtcgaa atcaggtgca
    53881 atagagattg cgcggttcgc aggcaaagtc cacggcgtag tggtccagat caccagcgag
    53941 attgggccgt taacgttgct tacgccaaat ttcgctttca gcgcatcctg atcgaccgcc
    54001 tggaaagcga cgtcaatgga cggagaagtt ttgtcgtaat actcaacttc cgcttctgcc
    54061 agtgcagaac ggcagtctac gcaccagtgc accggcttcg cgcctttgtg caggtgacca
    54121 ttgccgatga ttttgcccag cgcgcggatg atgttggctt cagttttgaa gtccatagtc
    54181 agatacgggt gcgaccagtc gcccagcacg cccagacgga taaagtcttt gcgttgaccg
    54241 tcaacctggg tcgccgcgta ttcgcggcac ttggcgcgga actcggcggc ggtgaatttc
    54301 tcacccggct taccgtattc ttgctctact ttcagctcga tcggcagacc gtggcagtcc
    54361 cagccaggca catacggcga gtcataaccg gaaagccctt tggacttcac gataatgtct
    54421 ttcagaatct tgttaaccga gtgaccaata tgaatgctgc cattcgcata aggagggcca
    54481 tcatgcagaa tgaaggtttt tttgcctttt ttagccgcac ggatgatgcc gtacagatca
    54541 tcatcagtcc aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc acgcatcggg
    54601 aaccctgttt ccggcaaatt cagggttgat ttatagtcac tcatcagatt ctcggttccg
    54661 tgtttcggtt tgattacata acaggcttaa gccggttttg ttagcccaaa aaattcgcgg
    54721 gcggttaatt catcacgcgc aatctgcgct ttcagttcgt ccagcgacgc aaatcgctgc
    54781 tcattgcgta tttttttacg cagcactact tgtatatggc gaccgtaaag gtccattgca
    54841 acatctaaca aatgcacttc cagctgctgg cgaataccgg caaccgttgg gcgtgttccg
    54901 atgtttgcca cgccgggtaa cggcttttca ccgaggccca gcacttctac cgcataaacc
    54961 cctttcaccg gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa acctatagtg
    55021 cgccctaatt catcaccgtg gactacacgc ccggagatgg caaacgggtg ccccagtaaa
    55081 ctttctgcca gagccagatt gtcatcagcc agcgcctgac gcacggcggt gctgctgata
    55141 cgcacaccac cttcgcaaaa agtttgcgta ctggtgatat cgaagccgta ttccatgcca
    55201 gctttctgta ataacaagaa atcgccttca cgaccagcgc caaagcggaa atcatcacct
    55261 acggcaagaa attttacgcg caaatgcttc accaaaagat cgctgatgaa attttgcgcg
    55321 gttaacgccg caaaacgcct gtcgaaacgc acgcacagca cgtaatcaac gccacactct
    55381 gcaaggtaac gcagtttttc ccgcagccgg gtcagtcttg ccggggcttt atcggtagca
    55441 aacagttcca gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt gcgcttgcgc
    55501 ccttcttcct gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc gtcgaaatta
    55561 ccaatagtca gcacacaccc ttcttgcggg gcctggctga gattatgtat gccgcgtatc
    55621 agcttcatgt ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta cagcggtcaa
    55681 ggttaaccgg cgattgagta ccgaaatcag aaagaagtgg cttttcattg ccatggcgca
    55741 aatcacggga agaaactgac cgcctgctgc aatttttatc gcggaaaagc tgtattcaca
    55801 ccccgcaagc tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg
    55861 cgcttatttg cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt
    55921 gaattgtcca tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag
    55981 aagcgcgcca ttcagtctga aaaggct
//
LOCUS       DAJTYI010000011        52364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYI010000011 DAJTYI010000000
VERSION     DAJTYI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..284)
                     /gene="atl"
                     /locus_tag="OO564_000661"
     CDS             complement(<1..284)
                     /gene="atl"
                     /locus_tag="OO564_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein"
                     /protein_id="HCR6651908.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQI"
     gene            447..543
                     /gene="ffs"
                     /locus_tag="OO564_000662"
     ncRNA           447..543
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO564_000662"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            663..1016
                     /locus_tag="OO564_000663"
     CDS             663..1016
                     /locus_tag="OO564_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6651909.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1058..2608)
                     /gene="pdeB"
                     /locus_tag="OO564_000664"
     CDS             complement(1058..2608)
                     /gene="pdeB"
                     /locus_tag="OO564_000664"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6651910.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2772..3242)
                     /locus_tag="OO564_000665"
     CDS             complement(2772..3242)
                     /locus_tag="OO564_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6651911.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3358..3909)
                     /gene="maa"
                     /locus_tag="OO564_000666"
     CDS             complement(3358..3909)
                     /gene="maa"
                     /locus_tag="OO564_000666"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6651912.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4081..4299)
                     /gene="hha"
                     /locus_tag="OO564_000667"
     CDS             complement(4081..4299)
                     /gene="hha"
                     /locus_tag="OO564_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6651913.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4325..4699)
                     /gene="tomB"
                     /locus_tag="OO564_000668"
     CDS             complement(4325..4699)
                     /gene="tomB"
                     /locus_tag="OO564_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6651914.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5245..8394)
                     /locus_tag="OO564_000669"
     CDS             complement(5245..8394)
                     /locus_tag="OO564_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6651915.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8417..9610)
                     /gene="acrA"
                     /locus_tag="OO564_000670"
     CDS             complement(8417..9610)
                     /gene="acrA"
                     /locus_tag="OO564_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6651916.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9752..10399
                     /gene="acrR"
                     /locus_tag="OO564_000671"
     CDS             9752..10399
                     /gene="acrR"
                     /locus_tag="OO564_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6651917.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10527..13889
                     /gene="mscK"
                     /locus_tag="OO564_000672"
     CDS             10527..13889
                     /gene="mscK"
                     /locus_tag="OO564_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6651918.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14101..14262)
                     /gene="rsmS"
                     /locus_tag="OO564_000673"
     CDS             complement(14101..14262)
                     /gene="rsmS"
                     /locus_tag="OO564_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6651919.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14276..14803)
                     /gene="priC"
                     /locus_tag="OO564_000674"
     CDS             complement(14276..14803)
                     /gene="priC"
                     /locus_tag="OO564_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6651920.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14873..15250
                     /locus_tag="OO564_000675"
     CDS             14873..15250
                     /locus_tag="OO564_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6651921.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15403..15954
                     /gene="apt"
                     /locus_tag="OO564_000676"
     CDS             15403..15954
                     /gene="apt"
                     /locus_tag="OO564_000676"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6651922.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16083..18008
                     /gene="dnaX"
                     /locus_tag="OO564_000677"
     CDS             16083..18008
                     /gene="dnaX"
                     /locus_tag="OO564_000677"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6651923.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17341..17405
                     /gene="dnaX"
                     /locus_tag="OO564_000677"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18061..18390
                     /locus_tag="OO564_000678"
     CDS             18061..18390
                     /locus_tag="OO564_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6651924.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18390..18995
                     /gene="recR"
                     /locus_tag="OO564_000679"
     CDS             18390..18995
                     /gene="recR"
                     /locus_tag="OO564_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6651925.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19105..20979
                     /gene="htpG"
                     /locus_tag="OO564_000680"
     CDS             19105..20979
                     /gene="htpG"
                     /locus_tag="OO564_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6651926.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21160..21804
                     /gene="adk"
                     /locus_tag="OO564_000681"
     CDS             21160..21804
                     /gene="adk"
                     /locus_tag="OO564_000681"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6651927.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21936..22898
                     /gene="hemH"
                     /locus_tag="OO564_000682"
     CDS             21936..22898
                     /gene="hemH"
                     /locus_tag="OO564_000682"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6651928.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22895..23855)
                     /gene="aes"
                     /locus_tag="OO564_000683"
                     /pseudo
     CDS             complement(22895..23855)
                     /gene="aes"
                     /locus_tag="OO564_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24007..25311
                     /gene="gsk"
                     /locus_tag="OO564_000684"
     CDS             24007..25311
                     /gene="gsk"
                     /locus_tag="OO564_000684"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6651929.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25444..27120)
                     /locus_tag="OO564_000685"
     CDS             complement(25444..27120)
                     /locus_tag="OO564_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6651930.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27358..28578)
                     /gene="fsr"
                     /locus_tag="OO564_000686"
     CDS             complement(27358..28578)
                     /gene="fsr"
                     /locus_tag="OO564_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6651931.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28796..30448
                     /gene="ushA"
                     /locus_tag="OO564_000687"
     CDS             28796..30448
                     /gene="ushA"
                     /locus_tag="OO564_000687"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6651932.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30485..30964)
                     /gene="ybaK"
                     /locus_tag="OO564_000688"
     CDS             complement(30485..30964)
                     /gene="ybaK"
                     /locus_tag="OO564_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6651933.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31168..31962)
                     /locus_tag="OO564_000689"
     CDS             complement(31168..31962)
                     /locus_tag="OO564_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6651934.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32100..32441
                     /locus_tag="OO564_000690"
     CDS             32100..32441
                     /locus_tag="OO564_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6651935.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32656..35160)
                     /gene="copA"
                     /locus_tag="OO564_000691"
     CDS             complement(32656..35160)
                     /gene="copA"
                     /locus_tag="OO564_000691"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6651936.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35422..36354
                     /gene="glsA"
                     /locus_tag="OO564_000692"
     CDS             35422..36354
                     /gene="glsA"
                     /locus_tag="OO564_000692"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6651937.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36357..37649
                     /locus_tag="OO564_000693"
     CDS             36357..37649
                     /locus_tag="OO564_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6651938.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37774..38181
                     /gene="cueR"
                     /locus_tag="OO564_000694"
     CDS             37774..38181
                     /gene="cueR"
                     /locus_tag="OO564_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6651939.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38182..38640)
                     /locus_tag="OO564_000695"
     CDS             complement(38182..38640)
                     /locus_tag="OO564_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6651940.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38637..39554)
                     /locus_tag="OO564_000696"
     CDS             complement(38637..39554)
                     /locus_tag="OO564_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6651941.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39700..40377
                     /gene="fetA"
                     /locus_tag="OO564_000697"
     CDS             39700..40377
                     /gene="fetA"
                     /locus_tag="OO564_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6651942.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40364..41143
                     /gene="fetB"
                     /locus_tag="OO564_000698"
     CDS             40364..41143
                     /gene="fetB"
                     /locus_tag="OO564_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6651943.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41206..42060)
                     /gene="cnoX"
                     /locus_tag="OO564_000699"
     CDS             complement(41206..42060)
                     /gene="cnoX"
                     /locus_tag="OO564_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6651944.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42121..42930)
                     /gene="ybbO"
                     /locus_tag="OO564_000700"
     CDS             complement(42121..42930)
                     /gene="ybbO"
                     /locus_tag="OO564_000700"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6651945.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42920..43542)
                     /gene="tesA"
                     /locus_tag="OO564_000701"
                     /pseudo
     CDS             complement(42920..43542)
                     /gene="tesA"
                     /locus_tag="OO564_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43513..44199
                     /locus_tag="OO564_000702"
     CDS             43513..44199
                     /locus_tag="OO564_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6651946.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44196..46610
                     /locus_tag="OO564_000703"
     CDS             44196..46610
                     /locus_tag="OO564_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6651947.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47322..48416)
                     /gene="mnmH"
                     /locus_tag="OO564_000704"
     CDS             complement(47322..48416)
                     /gene="mnmH"
                     /locus_tag="OO564_000704"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6651948.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48485..49411)
                     /gene="allS"
                     /locus_tag="OO564_000705"
     CDS             complement(48485..49411)
                     /gene="allS"
                     /locus_tag="OO564_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6651949.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49641..50123
                     /gene="allA"
                     /locus_tag="OO564_000706"
     CDS             49641..50123
                     /gene="allA"
                     /locus_tag="OO564_000706"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6651950.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50201..51016
                     /gene="allR"
                     /locus_tag="OO564_000707"
     CDS             50201..51016
                     /gene="allR"
                     /locus_tag="OO564_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6651951.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51106..>52364
                     /gene="gcl"
                     /locus_tag="OO564_000708"
     CDS             51106..>52364
                     /gene="gcl"
                     /locus_tag="OO564_000708"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6651952.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGP"
BASE COUNT        12614 a        13530 c        13781 g        12439 t
ORIGIN      
        1 tcgatttgcc cgcttcccga taccatcaca ccttctgcca gtaatgcctg tcgctgacgc
       61 tgtaaatccg gcccggttag cgaaattgtg ccatggcgat taaccacccg gtgccagggt
      121 aaggtgctgc cttcagggag acgctttaac acaccgccca cctggcgcgc ggcgcggggc
      181 gatcccgcca gtttcgccac atcaccgtaa gtggtgacat agccttcggg aatagcggcg
      241 acgatttgcc agacgcgttg gggaaatgaa tcttcttttt ccatcttttc ttcctgaggt
      301 aatttttcag cataatctgg aaaaacgccc gagtgaagtc gcattgcgca agaaaccagc
      361 atctggcacg caatgggttg caattagccg gggcagcagt gataatgcgc ctgcgcgttg
      421 gttttcaacg ctctcaatgg gggctctgtt ggttctcccg caacgctact ctgtttacca
      481 ggtcaggtcc ggaaggaagc agccaaggca gatgacgcgt gtgccgggat gtagctggca
      541 gggcccccac ccatttctgc ctcccaccgt ttcgtcaaaa aatcccagca tggctaaact
      601 ttaaccacaa ctgacgtcgc aagaattgtc tggctgcgca gtacgcttcg gaggtatgtc
      661 tgatgaagta tgttgatggt tttgtggttg ccgttcctgc cgataaaaag gatgcctatc
      721 gggaaatggc cgctaaggcc gcgccattgt ttaaagagtt tggcgcgctt cgtattgtcg
      781 aatgctgggc cagcgatgta ccggatggca aagtgaccga ttttcgtatg gcggtgaaag
      841 cggaagagaa tgaagaggtt gtctttagct ggattgaata cccttcaaaa gaggtccgcg
      901 acgcagctaa tcaaaagatg atgtcggacc cacggatgaa agagttcggc gagtccatgc
      961 cgtttgacgg caagcgaatg atctatggcg gattcgagtc aatcatcgac gaataggtag
     1021 cgtgacgggc tgtgcggcgt gccgtcagcc caaaaattca caaatgttgc tcggcccagc
     1081 gtaagaaatc ttctttcggt aatgccttgc tgtagagcca gccctgaccg tagtgcacgc
     1141 catgctggcg tagccactct tcttgtttac tggtttcgat tccctccgct accattttca
     1201 gtttcagtgt ttttgccatt tcgatgatat gcggcgtgac atttttatat tccagcgcat
     1261 caacgaaaga tttatcgatc ttcagaatgt cgacatccag atcctgtaaa taacttaaac
     1321 ttgaataccc cgtaccaaaa tcatcaagat aaatttcatg gcccgcctcc cggtagcgag
     1381 aaattatcgg ggcgctggtt ttcggatcgg caaactcgcg ttcagtgagt tcaagcgcga
     1441 tctgtctggg attaacctga tagtgattga tcatttcacg cagcaattgc gggatttttt
     1501 ccgaggtgag cacggtggat tcaagattga tcgaaatatg ctgctgtgga tgctgacgca
     1561 agcagtcgcc catatcttca aagacgcttc ttataatcag tagcgtcaat ggctcagaaa
     1621 ggcccgtttg ctgtgccagc ggaataaaac tatctggtga caaccaacta ccgtctgtct
     1681 gcggccagcg cgccagtgcc tcagcaccga caattttgcc attggctaag gagacaatcg
     1741 gctgatagtg cacgcaaata tcacgatttt cgatagcatc ctgcagccga tgatgcggtg
     1801 actggatacg gcgcaaaata cgcagcacaa acatcgctgc cagcaggcca atcaccaacc
     1861 cgagcggtaa ccaaataaag acttgccgat gccagccttt ctgcaacatt ttcgttgaag
     1921 cccaggtgat gatcgaaata ttcatctccg ggaagggcag gatatcgtaa atgatcccat
     1981 tattttcgat atgctcaccg ggtgtttttt gtagcctggt aataattccc tgagcaattt
     2041 catcgctgct ggttatgaca acgttatggg cattgccaat aatggcggca tcaatttgcc
     2101 atgagctata gggaatgaca tcaataaagg aagcggggtc gatcatgacg acataatgtg
     2161 ccgttcccat ggcgaccatg taacggataa tgcctaaatc gttatgcgat gttaaccaga
     2221 cacgataacc atctttcgaa attttacctg gctcgggaaa ggtatcgggc ggactttcat
     2281 gctccagaga cgaacactgg ggaacgttgt tatcgatata aaccacttcc tgaatatagc
     2341 gataactgta agatacccga cgcatttcca tgagatgggc ttcgctacag gcagcgcctt
     2401 gccatctttc cagctcctgc agcgcatctt tcccttgtgt cgccacctta ttggctcgaa
     2461 tagcgacgcg ggaggaatag gtatccagct cttcaataaa cgatgtttct acctgctgat
     2521 gggccagcca gatgcttaag ccgacaggca gcaatactga aagaatcagt actcccgaaa
     2581 taaggccgac cagatgtcgt gttctcacca gcatatcctt attaacacag tgtagggaaa
     2641 gtatatctga ttgtggaaaa aggctggcag gagataagga ggtagagctg gtatttcatg
     2701 aagtgatggg tggaaacgat cattgtcatt tgaccaggcg cagagcttta cgcctggtat
     2761 gtaaccctaa cttaagatgt tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac
     2821 agttcacctt tacggacgat cattttttgc agttgttcct tatgaacatc cgcgacacgc
     2881 ggagagttca agatggcttc aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg
     2941 ccgttatagc aaacgcggaa atccagcacg tcgatatctt catagcggta gcgtgggtag
     3001 acatcgaaaa agaagaaacc attaagcagg ataaataata caaccagtag ccagacagag
     3061 cctgacagcg tttcggactg caacatcacc gccagggtgg caaaaaaagc aacgtacata
     3121 ccgataaaca gccccggatg tttacggata aaactgatac taaagcgggg tttgttgtcg
     3181 cgtttttcgc gggtattcag agactcaatc gtttcggtca gcaggcgttg tatttcggtc
     3241 atcgtttgcc ttcgtggtat ctgcaacttt ccagggaaca ccctatttta acgtgggaat
     3301 agtagagggg aagtaacaga tctaccacaa ttttgcataa cagttgcgaa aaaccgatta
     3361 caatttttta attattctgg ctggattacc gcccacgaca acgttgtccg ggacaccttt
     3421 tgtgacaact gcacctgaag ctaccacgac gttatcacca atggtcacac cagggttaat
     3481 gaccgcgcgt ccgccaatcc agacgttatt accgatggtg acgggtttcc ccagttcagc
     3541 accgctatta cgtgctacag ggtcgatggg atgtgttgcc gtgtagatat gaacgcctgg
     3601 tgccaacata cagttatcac cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa
     3661 gttggcgaaa aaattattgc cgagaaaaat gttatagcca taatcacagc gaaacgttgg
     3721 ctcaatataa gcctctgtca cctgaccgaa taaatcagcg agaatttgct ggcgtaatgt
     3781 gtgctcttcc gctagggaat gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc
     3841 gcgagataac gtctcatctg ccgagcgata caactcacca gcaatcatct tttctttttc
     3901 tgtgctcatt actgacctcc ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca
     3961 gtgaagagaa cgttctcatt acatctgtgt gataaagatc acatagggta tgtcttcatg
     4021 gcgaaaaagt ataaaattct taataaacag ccggttatag ctccgaaagc gaattattta
     4081 ttagcgaata aatttccata ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa
     4141 ttcggcgagg cggtgatctg cggctgagta aaataccgcc agttcattat ctgataattc
     4201 gtatttattt ttctcgataa cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg
     4261 cattaaataa tcggtttttg ttaaaggttt ttcggacata cttctaccca tggttgtaat
     4321 aattctaaca agataaactc gcaggattct ctttcgtcgc attgacaaaa caacggaata
     4381 gtcgattacc tgacttccgc catttctgaa gagcctgcat attaatacca taactgctga
     4441 acaacataaa ggtgtcatcc agatattcgt cgatctgctc aatgagctta ttgtcttcat
     4501 tatacttaat tttgtaatta agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact
     4561 ggaggttgat cgccgaggtt gggtcgttaa cccagccatg attgctttct tcaaggtttg
     4621 caagacagtc atgatacagg gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt
     4681 tgggtgagta ttcatccata acgcgtcccc ttcttagtgg ttgaactaac ggacaccttt
     4741 cgggatggaa aaaacttact gacctagact tgccctctcg tttgttagct taactataag
     4801 ccactctttg caggttttca tcgcattatt acgaaacatt acaattagga aggaatcggg
     4861 acaggactca ctttttcata ctagcactaa cccgcagcag gtaaaagcag tttgctcgaa
     4921 ctgttcataa catttcagga ttatcgccag atgaaattgg tgacactaat accaggattg
     4981 ctctgaatat gacgtaataa ccgaggaatg aataaagaat taccgcaaat aattaagaat
     5041 agcctctaaa tgattatgga ttattaaatc tattagcgct acttaatata aattaactaa
     5101 caatcagtat tttttatgaa ttttatacgt ggttaatact ggttttcgta tgagatcctg
     5161 agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag gccgcttacg
     5221 cggccttagt gattacatgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc
     5281 ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat
     5341 tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc
     5401 gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga
     5461 ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt caatcagacc
     5521 tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc
     5581 cgacaaccca atggttgtga gcagtcctac ttggaagtaa acgtcattgg tcaggccacg
     5641 gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa
     5701 cggagtcgac cagctctcgt acagcgccgc cagacacagg aatacgacaa tcaacgaaac
     5761 cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca
     5821 gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc
     5881 accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca ggccgttgta
     5941 acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg agaatggcac
     6001 catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta
     6061 tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac
     6121 atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag aaacacccag
     6181 cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt
     6241 tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca gctggttacg
     6301 cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc
     6361 ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt
     6421 gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc
     6481 cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct gaccacgtcc
     6541 cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttttttt ctttggtcag
     6601 atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg
     6661 cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg agcttggcag
     6721 acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacta ggtaacgccc
     6781 cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat
     6841 gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg
     6901 tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta ccgacagcgc
     6961 cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa
     7021 gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg
     7081 aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac
     7141 acgctccacg ttttctacca caacgatagc gtcatccacc aacaagccga tggcgagcac
     7201 catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt
     7261 cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag
     7321 gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt taaccacttc
     7381 gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacccga
     7441 cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc
     7501 gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc
     7561 gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca gcagtacgcg
     7621 ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg
     7681 cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag
     7741 ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt
     7801 tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt actgtgaacc
     7861 gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca tattcgccgc
     7921 tacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac
     7981 catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa cttcttgcgg
     8041 cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc
     8101 agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga
     8161 catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc
     8221 ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg ccggcggtgc
     8281 aatcgtagga tattgtgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat
     8341 gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta
     8401 acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct
     8461 ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca
     8521 gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc
     8581 caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg ccaactacca
     8641 gtacggtggc atcaccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt
     8701 ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc agagtgtgat
     8761 ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca acggtaacgt
     8821 cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca
     8881 ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca
     8941 ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg
     9001 ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gacttaccaa
     9061 tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc gcagtttcaa
     9121 cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc agagcctgat
     9181 cgtacccttg cttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca
     9241 attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca ctgtcgtatg
     9301 tcgcctgata ggtcgcagga tcaatctgat agagagagac accagcttcg atgtcgctac
     9361 cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc
     9421 ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg
     9481 ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc
     9541 ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt
     9601 ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa
     9661 taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat
     9721 gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca
     9781 agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc
     9841 atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg
     9901 gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat
     9961 tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag
    10021 agagatatta attcatgttc ttgaatccac ggtgacagaa gaacgacgtc gattattgat
    10081 ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca
    10141 acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga
    10201 agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta
    10261 tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga
    10321 agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa
    10381 tcctgctact aacgaataac cctgaatctg actccaggat ttttcctgga cattttcgtc
    10441 gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc
    10501 ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc
    10561 attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt
    10621 ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg
    10681 actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga
    10741 cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca gttcagctac
    10801 ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac
    10861 ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc
    10921 agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg
    10981 cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga
    11041 tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg
    11101 gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag gcgttgctga
    11161 atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg caggatacct
    11221 tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcat cagttgcagc
    11281 tgttgcaaga agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag
    11341 ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa caggaactgg
    11401 aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga
    11461 tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata
    11521 ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc
    11581 aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt
    11641 tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag agcgatgcgt
    11701 tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt
    11761 tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa cagttgggta
    11821 accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg agcgtatcga
    11881 aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac cgtccaatgg
    11941 actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa
    12001 tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gccggtttgc
    12061 cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac
    12121 agaaactggc ttccgctgtg ggttccctgc gtaacgatag ccagctcaac acaccaaaag
    12181 cgatccttat cgacctgatc cgcgcgctgc cggtgtgcct gattattctc gcggttggcc
    12241 tgattctgtt gaccatgcag ctcaatatca gcgaactgct atggtcgttc agtaaaaaac
    12301 tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa aatggcgttg
    12361 ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg caaattgtcc
    12421 gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa ctttccccgc
    12481 tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg ctgctgattg
    12541 ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc
    12601 gactagtcac tattaccgtg ctgtcgatta tcccgattgc attgatggtg ctgactgcca
    12661 cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg
    12721 tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta gcggcgcggc
    12781 gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag
    12841 gtgccgaacc gccggaagaa cccaccattg cactggagca agttaaccag cagacgctgc
    12901 gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg
    12961 atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac aacggcactg
    13021 aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg
    13081 cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa gtgctggtgc
    13141 tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat tactaccatc cttaactaca
    13201 tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctct tgggataaac
    13261 tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa gaaattttcg
    13321 gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg
    13381 taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta
    13441 ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga
    13501 tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc gtggcctatg
    13561 gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg
    13621 tgatgcacga accaatgccg gaagtcttct ttacggcatt tggtgccagc acgttggatc
    13681 atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc gatgagctga
    13741 accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg
    13801 aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtaaaacgc gactacaaag
    13861 gcgacgatcc gacaccagcg gttgggtaat aaccaaaggg ggcgacattt agttgccccg
    13921 agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc
    13981 tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata agcgtagcgc
    14041 atcaggcagt tttgcgtttg cccgcaatct taggggacat ttagcggccc catttatttc
    14101 tcacttttcc gcctcatcat cgtgcgttaa tttcttttca taatcacgct ttacaatatc
    14161 cagcgcgcgc agcacggtac tggcagggat ctgattttcc tccagcagca caatcaaatc
    14221 gacagccagt ttgacatcgt caggggcatt ttcaagtgac atactctttc cattgctagc
    14281 gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag cgtgccaggc
    14341 gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc
    14401 gcgccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca aaatcctgat
    14461 gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc cactcgcgca
    14521 atgaccaggc ggcggcttca cgcgcgatgg cttccagttg tgccgccaga tgttccgcca
    14581 gccaggccac ttgcggcagc tgttgctgct caactgcatg acgaagcgca gccagattat
    14641 cgcccgcctc gtcgagacaa gcctgtagtg ttgtcgcacg agtctgaaaa agatgcctgt
    14701 cgaaacgagc acttagcgtg gcgaactgtg acaccggggc acaacgctga cgcagcgtag
    14761 cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga cctcaatgaa
    14821 aatgataatt gttatgctaa agtagccact ctgtaagctg acaacactca atatgcaacg
    14881 aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt
    14941 accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg cccgttcttc
    15001 cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc tacgtttctg
    15061 gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct
    15121 cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt
    15181 aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg aaaagcaaga
    15241 aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt acattctggc
    15301 gttttcgagc acaggcgcag gcggtcaaag gttaaacaac ggttactttt gatacgttta
    15361 aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc gactgcacag
    15421 cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa acccggcatt
    15481 cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct cagcatcgac
    15541 ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt
    15601 ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt accggtccgt
    15661 aaaccgggca aactgccgcg tgaaaccatc agtgaaaatt acgacctgga atacggcacc
    15721 gatcagctgg aaatccacgt tgatgccatc aaaccgggcg acaaagttct ggtggtggac
    15781 gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg tcgtctgggt
    15841 ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg cgaacagcgt
    15901 ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca ttaattatcg
    15961 ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc tcgccgttct
    16021 gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt cagagcctgc
    16081 caatgagtta tcaggtttta gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg
    16141 gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt attcatcatg
    16201 cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga
    16261 aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc
    16321 gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca
    16381 aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg cgtggtcgtt
    16441 tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt
    16501 taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg accgatccac
    16561 agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag gcgctggatg
    16621 tcgagcaaat tcgccatcag cttgagcaca tcctcaatga agaacatatc gctcacgagc
    16681 cgcgggcgct gcaattgttg gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc
    16741 tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg gtcagtgcga
    16801 tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg gtcgagggca
    16861 acggcgagcg cgtaatggca ctgattaatg aagccgctgc ccgtggtatc gagtgggaag
    16921 cgttgctggt ggaaatgctc ggcttgttgc atcgtattgc gatggtacaa ctttcgcctg
    16981 ctgcacttgg caacgacatg gccgccatcg agctgcggat gcgtgaactg gcgcgcacca
    17041 taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc aaagaattac
    17101 cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc
    17161 atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca cccgtcgcgc
    17221 caacggcagt aatgacgcca accctggtgc cgccgcaatc agcgccgcag caggcaccga
    17281 ctgtaccgct cccggaaacc accagccagg tgctggcggc gcgccagcag ttgcagcgcg
    17341 tgcagggagc aaccaaagca aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg
    17401 tgaataacgc tgcgctggaa agactggctt cggtcaccga tcgcgttcag gcgcgtccgg
    17461 tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc gtatcgctgg aaggcgacca
    17521 ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg
    17581 aacatgaaaa aacgccggaa ctggcggcga agctagcggc agaagccatt gagcgcgacc
    17641 cgtgggcggc acaggtgagc caactttcgc taccaaaact ggtcgaacag gtggcgttaa
    17701 atgcctggaa agaggagagc gacaacgcag tatgtctgca tttgcgctcc tctcagcggc
    17761 atttgaacaa ccgcggcgca cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt
    17821 caacggttga actgactatc gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt
    17881 ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt attgcggata
    17941 ataatattca gaccctgcgt cggtttttcg atgcggagct ggatgaagaa agtatccgcc
    18001 ccatttgatc gtaagcacag cttacgttcg tcatccttaa cgtgattgag agagaaacct
    18061 atgtttggta aaggcggtct gggtaacctg atgaagcaag cccagcagat gcaagaaaaa
    18121 atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtta ccggcgaatc tggcgcaggt
    18181 ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc gcgtagagat cgacccgagc
    18241 ctgctggaag acgacaaaga gatgctggaa gacctggtgg ctgcagcatt caacgacgca
    18301 gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag
    18361 ctgccgcctg gctttaagat gccgttctga tgcaaaccag cccgctgtta acacagctta
    18421 tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca
    18481 cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc gcaggcgctc acccgggcga
    18541 tgtcggaaat cggccactgc gccgattgcc gcactttcac cgaacaggaa gtctgtaaca
    18601 tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag agtccggcgg
    18661 acatctacgc cattgagcag acggggcagt tttctggtcg ttattttgtg ttgatggggc
    18721 atctgtcacc gctggacggc atcggtccgg atgatatcgg gcttgatcgt ctggaacagc
    18781 gtctggcaga ggaaaaaatc actgaagtga tcctcgccac caatcccacg gttgaaggtg
    18841 aagctaccgc taactacatt gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa
    18901 tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc acgctgtcac
    18961 actccctcgc cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg agcacctgct
    19021 ctcgcttgaa attattctcc cttgtcccca tctctccctc atcctgtttt taaccttaaa
    19081 atggcattat tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa
    19141 gtgaaacagc ttctgcacct gatgatccat tctctctatt ccaataaaga aatcttcctg
    19201 cgtgagctta tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct
    19261 aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac
    19321 aagcgcacgc tgaccatctc cgataacggc gtggggatga cccgcgacga agtgattgac
    19381 catctgggga ctatcgctaa atccggtacc aaatcattcc tcgaatccct gggttctgac
    19441 caggcgaaag acagccagct gatcggtcag tttggtgttg gtttctactc tgcgtttatc
    19501 gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc
    19561 ttctgggaat cggctggcga aggtgaatat accgttgccg acatcaccaa agaagatcgt
    19621 ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc
    19681 gtgcgttcca tcatcagcaa atactccgac catatcgcgc tgccggtaga gatcgaaaaa
    19741 cgcgaagaga aagatggcga aaccgttatc tcctgggcaa aaatcaacaa agcgcaggcg
    19801 ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt ataaagagtt ctacaaacac
    19861 atcgcccacg actttaacga tccgctgacc tggagccaca accgcgttga aggtaagcag
    19921 gagtacacca gcctgctgta catcccgtcc caggctccgt gggatatgtg gaaccgtgat
    19981 cataaacacg gcctgaaact gtacgttcaa cgcgtgttta tcatggacga cgctgaacag
    20041 ttcatgccga actatctgcg cttcgtgcgt ggtctgattg actccagcga tctgccgctg
    20101 aacgtttccc gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg caatgcgctg
    20161 accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga aaaataccag
    20221 accttctggc aacagtttgg cctggtactg aaagaaggtc cggcggaaga tttcgctaac
    20281 caggaagcga tcgccaaact gctgcgtttt gcttctactt ataccgattc ttctgcgcag
    20341 accgtgtctc tggaagacta cgtttcccgc atgaaagaag ggcaggagaa aatctactac
    20401 atcactgcag acagctatgc ggcagcgaag agcagcccgc acctggaact gctgcgtaag
    20461 aaaggcatcg aagttctgct gctttccgac cgcatcgatg agtggatgat gaactatctg
    20521 actgagttcg acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa
    20581 ctggctgacg aagttgatga gagcgcgaaa gaagcggaga aagcactgac tccgttcatc
    20641 gaccgcgtga aagctctgct cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg
    20701 accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa
    20761 ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat acatcttcga actgaacccg
    20821 gatcacgtac tggtgaaacg tgcggcagat actgaagatg aagccaagtt cagcgagtgg
    20881 gtagaactgc tgctggatca ggcgctgctg gcagaacgcg gcacgctgga agatccgaac
    20941 ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat gtcatgccgg atgacctttg
    21001 tgtcatccgg catttttctt ttcatcatct gtactttccg caaattatct cgccattaac
    21061 cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct
    21121 ttttcaaaaa aatcgacaca ttttaagggg attttcgcaa tgcgtatcat tctgcttggc
    21181 gctccgggcg cggggaaagg gactcaggct cagttcatca tggagaaata tggtattccg
    21241 caaatctcca ctggcgatat gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa
    21301 caagcaaaag acattatgga tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg
    21361 gttaaagagc gcattgctca ggaagactgc cgtaatggtt tcctgttgga cggcttcccg
    21421 cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg
    21481 gaattcgacg taccggacga actgattgtt gatcgtatcg taggccgccg cgttcatgcg
    21541 ccgtctggtc gtgtttatca cgttaaattc aatccgccga aagtagaagg caaagacgac
    21601 gttaccggtg aagaactgac tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt
    21661 ctggttgaat accatcagat gactgcaccg ctgatcggct actactccaa agaagcggaa
    21721 gcgggtaaca ccaaatacgc gaaagttgac ggtaccaagc cggttgctga agttcgcgct
    21781 gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc
    21841 tcatccacct caggcaatac ctctcacagc aattagttct tcttcctcac ttttccgcta
    21901 caattatcaa caagttgaat cgataagagg cggtaatgcg tcagactaaa accggtatcc
    21961 tgctggcaaa cctgggtacg cccgatgccc ccacacctga agcggtaaaa cgctatctga
    22021 aacaattttt aagcgacaga cgcgtggttg atacctcacg gttgttatgg tggccgttgc
    22081 tgcgcggcgt gattttgccg ctgcgctcgc cgcgtgtggc gaagctgtac gcctctgtct
    22141 ggatggaaga cggctcgccg ctgatggttt acagccgcca gcaacagcag gcgttggcac
    22201 aacgtttacc ggatacgcca gtggcgctgg gcatgagcta cggctcgcca tcactggaaa
    22261 gcgccgtcga tgaactcctg gcagagcatg tagatcatat tgtggtgctg ccgctttatc
    22321 cgcaattctc ctgttctacg gtcggtgcgg tatgggatga actggcacgc attctggcgc
    22381 gcaaacgtag cattccgggg atatcgttta ttcgtgatta cgccgataac cacgattaca
    22441 ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa ccggatctgc
    22501 tgctgctctc ttatcatggc attccccagc gttatgcaga tgaaggcgat gattacccgc
    22561 aacgttgccg cacaacgact cgcgaactgg cttccgcact ggggatggta ccggaaaaag
    22621 tgatgatgac ctttcagtcg cgctttggcc gggaaccctg gctgatgcct tataccgacg
    22681 aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat acaggtgatg tgcccgggct
    22741 ttgctgcgga ttgtctggag acgctggaag agattgccga gcaaaaccgt gaggtcttcc
    22801 tcggtgcggg cgggaaaaaa tatgaatata ttccagcgct taatgccacg ccggaacata
    22861 tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag ctgagcggta aagaactgag
    22921 cgccgtcgcg aagagcctcg tcggcggttt tcatcatccg tgaataatgc aaaaaggcat
    22981 gcagcgtacc tgggtagagt ttgaactcac agggctgctg atgcgccgct aacgtctggt
    23041 aaagcagacg gctgtcatcc agcagcggat cgaactcctc cccggcaata aaacagggcg
    23101 gaacttcgcg agtgagatca ttattaaaca gacagtaata cggtgactcg cggtccgcgt
    23161 cgttgcttaa atacgcctct tcatacatct gcaaatcctg ttgcgttaag ccatcccaga
    23221 caccgcccaa cagacgacga gtcacggaat cccgtaatcc gtaaagccca taccacagca
    23281 aaacacccgc aattttaccg caatcgatct gtttatcacg caaccacaac gcactggcga
    23341 gcgccagcat ggcacctgcg gaatcaccgg caaagccaat gcgggacata ttgatttgat
    23401 aatcctccgc ctgctggtgg aaataacaac aagcagccac aatttcctct atcgcttgtg
    23461 gaaaacgcgc ttcaggtgaa agggtgtaat caataccaat caccgtacat tggctgtagc
    23521 ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt gccgagaata aaaccgcctt
    23581 ccatgcaaat aaaatagcgt cgccgggcta tctggctgcg gacaaaagag acgtgtttcc
    23641 acctgcccat attttgttgg aaccatgtaa gctctggttg ccatttctgg agcgcccgca
    23701 ttccagaatc ggcgctcaag cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg
    23761 ggccaggacg gtaaatccgg ctgaagagta ttcacaacgg tcttcatttc agcagaaata
    23821 aggtccagaa caggtagttt gttttccggc ttcatacaaa aactcctttc aaattacgtc
    23881 attgtaagga accactgcca tgaaaatgcg atcccgcctg ctgatattga aactggctgc
    23941 gtctcgcgcg ctcccgtcag attgtgttaa cattcgccgc tcagttaacc acccgtaaaa
    24001 acaaccatga aatttcccgg taaacgtaaa tccaaaaatt acttccctgt aaatgcacgc
    24061 gatccgctgc ttcagcaatt ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt
    24121 attgaccaga cgctggtcga tattgaagcg aaagtggatg atgaattcat tgagcgttat
    24181 ggattaagcg ccgggcattc actggtgatt gaggatgacg tagccgaagt gctttatcag
    24241 gagctaaaac agaaaaacct gattacccat cagtttgcgg gtggcaccat tggtaacacc
    24301 atgcacaact actcggtgct cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc
    24361 aatattgaaa ttggcagtta tgcctatcgt tacctgtgta acacttccag ccgtaccgat
    24421 cttaactatc tacaaggcgt ggatggtccg attggtcgtt gctttacgct gattggcgag
    24481 tccggggaac gtacctttgc tatcagtcca ggccacatga accagctgcg ggctgaaagc
    24541 attccggaag atgtgattgc cggagcctcg gcactggttc tcacctcata tctggtgcgt
    24601 tgcaagccgg gtgaacccat gccggaagca accatgaaag ccattgagta cgcgaagaaa
    24661 tataacgtac cggtggtgct gacgctgggc accaagtttg tcattgccga gaatccgcag
    24721 tggtggcagc aattcctcaa agaccacgtt tctatccttg cgatgaacga agatgaagcc
    24781 gaagcgttga ccggagaaag cgatccgttg ttggcatctg acaaggcgct ggactgggta
    24841 gatctggtgc tgtgcaccgc cgggccaatc ggcttgtata tggcgggctt taccgaagac
    24901 gaagcgaaac gtaaaaccca gcatccgctg ctgccgggcg ctatagcgga attcaaccag
    24961 tatgagttta gccgcgccat gcgccacaag gattgccaga atccgctgcg tgtctattcg
    25021 cacattgcgc cgtacatggg cgggccggaa aaaatcatga acactaatgg agcgggggat
    25081 ggcgcattgg cagcgttgct gcatgacatt accgccaaca gctaccatcg tagcaacgta
    25141 ccaaactcca gcaaacataa attcacctgg ttaacttatt catcgttagc gcaggtgtgt
    25201 aaatatgcta accgtgtgag ctatcaggta ctgaaccagc attcacctcg tttaacgcgc
    25261 ggcttgccgg agcgtgaaga cagcctggaa gagtcttact gggatcgtta agttatcgtc
    25321 ggttcgtagg ccagataagg cgttcacgcc gcatctggca tttggctctc gatgcctgat
    25381 gcgacgctgg cgcgtcttat catgcctaca tatttttcat attttacatc cggcaaccac
    25441 cgtttacccc gtcaccactt cacccgccgg tggcgtttcc agcagttcca gcatagtacg
    25501 ggcaatttca cgttcaccca tcactacctg attcgcgcca cgttcggtga tatacgtcac
    25561 ttcatcgtca taatgggcgc gggcgataat ctcaatatcc gggtttttcg cgcgggcaga
    25621 cgccacaatc tcacccgctt cataaccgtt ggggatcgtc aggatcagcc attttgcaca
    25681 ttccagatgt gccagttgca taatttcttc gttcgctgca ttgcccaata cagcgcggac
    25741 cccacgctct cgcagctcat caacacgggt tcgtgacgtc tcaatcacca ccagcggaat
    25801 atcagaggcg agcaatttct cccccagcag gctgcctaca cgaccgtaac ccaccaatag
    25861 cgcatggttg caaatatcca ctgggatctg tttctcttct tcgattgcct cttccagcgt
    25921 ctgctcttcc agcgtttcgg tcttcgccag atatttctcc agtagtgcga acagtaccgg
    25981 gttgagcata atcgacagga tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag
    26041 taaattcaat gccattccca gtcccgccag gataaacgcg aactcaccaa tctgcgccag
    26101 gctggcggcg atggttaatg ccgtacgttg ggagtgacca aacagacgga caaggaacag
    26161 ggccgcgacc gatttaccaa acagaataat tgccagcgtc gccagcactg ccagcggttg
    26221 ctgaatcaga attaacggat caaacaacat cccgactgag acaaaaaaca gcaccgcaaa
    26281 cgcgtcgcgc aatggcaacg tatcgtgggc ggcacggtga ctcaattcag actcgttcag
    26341 taccatcccg gcaaagaacg caccgagtgc aaaggagaca tcaaacagct ctaccgcacc
    26401 aaaggcaatc cctaacgcca gcgccagcac tgatagggta aacagttcgc gagaaccggt
    26461 tgccgcgctg cgtgccataa tccacggcac cagacggcga cctaccagca tcataatggc
    26521 gataaatgcg atcaccttgc cgatggtgat ccccatatcg actgcaagag tggcaaagcc
    26581 cacatcgccc tgttccatca ttcctgccac tgcgggcagc aacaccagcg tcagaaccat
    26641 caccaggtct tccacaatca accaaccgat ggcgattttc ccacgctgac tgtctattaa
    26701 ttgccgttct tcaagtgcgc gcagtaacac cacggtactg gcggtggaaa gacataagcc
    26761 gaacacgata ccggtcatta acgaccagcc cagcacggcg gagagcgcca tacccagcag
    26821 cgtcgccacg gctatctggg cgatcgcgcc gggaatggcg atggacttta ccgccatcaa
    26881 atccttgagc gaaaaatgca aaccgacgcc gaacatcagc aaaataacgc ccagttcagc
    26941 cagttccggc gcaagcttgg tatcggcaac aaagcccgga gtgaatggtc ctgccagcac
    27001 acccgctaac agatatccca ccagaggaga aatacgtagt ttattggcca gcatgccgag
    27061 gataaaggcg agcacaaggc cgccaacaat ggtggtgata agcggggtgg cgtgatgcat
    27121 tccgtctcct tttcctggtg gttattgtcc atttttggcc gggaaaacca aaattacagg
    27181 taatagttta tgacaatttc attgatgatg tttatgaata attgttgaat tttgcagaaa
    27241 aatggaatta gctgcaaaaa aagcacggat cggaaaacag aaggcggttt aacagaggaa
    27301 aggcttatgg cgactgtaaa acgatgcagc caaagtttgc ctttggctgc aatgaaatca
    27361 gtctttatgc cggttatcag gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc
    27421 acagatttta tagactaact cgatgctggt gtgatcggcg ataagcccca gaactgccgc
    27481 tcccagacct cccatgccga aagcaaaacc gaaaaagagt ccagaaatca taccgatacg
    27541 tcccggaagt agctcctgag cgtagaccag aatggcagag aatgccgaag cgaggataaa
    27601 tccaataatc accgttaaaa ctcccgtcca gtgcaggcgg gcgtagggta aaatcagcgt
    27661 aaacggcgca acgccgagga tagagcccca aatcacatat ttccgtccaa ttttatcccc
    27721 tacaggcccg ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa acagatgaag
    27781 ctgggcattc tggatagata atccgaattt ttgcatcaga taaaaggtgt aatagctgct
    27841 gatgctcgcc atatagaaat atttcgagaa aatgaggatt aacagaatgc tgactgccag
    27901 taccactttg ttgcgcggca gtggattgat tatcgtcgct ttgggttttc ctttattcat
    27961 tcggtgctgt gccgagtacc aacggctaat ttgcgccaac accacgatcg ccagcagtgc
    28021 cgcaagcaca aaccaggcaa cgttgccttt gccataaggc gcgataatca ccgccgccag
    28081 caaggggccc tgggaactgc caaagttgcc gccgacctga aagatagatt gcgccaggcc
    28141 atgccgcccg ccggaagcca tacgggctac gcgagaagat tccggatgaa agaccgatga
    28201 accggtaccg accagcgccg ccgccagcag aactgcgcca aaactgcccg ccagcgcaag
    28261 cagcaccaga ccacttaagg taaagcacat gccaattggc aacgaccacg gcatcggata
    28321 tttatcggtc cagtagccga ccactggttg cagtagcgaa gaggcgagct ggaaggtgag
    28381 ggttatcatg ccaatctgca taaatgtcag agaaaattct gactgaagca gcggataaat
    28441 cgccagaatc agcgattgga tcatgtcgtt cagcagatgt gagaggctga tagcacctaa
    28501 aataccaaac gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt
    28561 ttgttcactc attgccatag gaaagtcact ttttcagggt tgcgatgtaa agaatgatct
    28621 tatttgtgat tattacccga ctaacatacc tgtatgcgtc gtctgaagga agtctcaacg
    28681 ccgaatacag aatttctaat ctggatgcag atttatcttc accggacgca gacttgtcta
    28741 tgatgtcgcg tcatactatt ttttaacacg ttgaaatcag gtcagggaga gaagtatgaa
    28801 attattgcag cggggcgtgg cgttagcgct gttaaccaca tttacactgg cgagtgaaac
    28861 tgctctggcg tatgagcagg ataaaaccta caaaattaca gttctgcata ccaatgatca
    28921 tcatgggcat ttttggcgca atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct
    28981 ggtggatggt atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc tactttccgg
    29041 tggcgacatt aacactggcg tgcccgagtc tgacttacag gatgccgaac ctgattttcg
    29101 cggtatgaat ctggtgggct atgacgcgat ggcgatcggt aatcatgaat ttgataatcc
    29161 gctcaccgta ttacgccagc aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat
    29221 ctaccagaaa agtactggcg agcgcctgtt taaaccgtgg gcgctgttta agcgtcagga
    29281 tctgaaaatt gccgttattg ggctgacaac cgatgacaca gcaaaaattg gtaacccgga
    29341 atacttcact gatatcgaat ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga
    29401 gctgcaacag acagaaaagc cagacattat tatcgcggcg acccatatgg ggcattacga
    29461 taatggtgag cacggctcta acgcaccggg cgatgtggag atggcacgcg cgctgcctgc
    29521 cggatcgctg gcgatgatcg tcggtggtca ctcgcaagat ccggtctgca tggcggcaga
    29581 aaacaaaaaa caggtcgatt acgtgccggg tacgccatgc aaaccagatc aacaaaacgg
    29641 catctggatt gtgcaggcgc atgagtgggg caaatacgtg ggacgggctg attttgagtt
    29701 tcgtaatggc gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa
    29761 agtgacctgg gaagacggga aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa
    29821 ccagcaaatg atctcgctgt tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt
    29881 gaaaataggc aaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca
    29941 gaccaatatg gggcggttga ttctggcagc ccaaatggat cgcactggtg ccgactttgc
    30001 ggtgatgagc ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca gctataaaaa
    30061 cgtgctgaaa gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga
    30121 ggtgattgat tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca
    30181 atttgccaac gttagctttg tggcgaaaga cggcaaactg aacgacctta aaatcaaagg
    30241 cgaaccggtc gatccggcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg
    30301 cggtgatgga tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga
    30361 tgccgaagtg ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga
    30421 accgaaaggt gaggtgagct ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca
    30481 atgcttaatc gcggcgggcg atatcagcaa atttggcatc gaggatcttt gccagatcgc
    30541 ctgccgccag ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt
    30601 cttgtgcggg ggcgtcgata atcgtgggca gacgtttttt ctgccccagt gggctaatcc
    30661 ccccaaccag gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct
    30721 tggcacccag cgcttttgct acttttttaa gatccagttg acctgcgacc ggcgtaacgg
    30781 ccacggcaag gtgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat
    30841 ccggattcaa acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt
    30901 gctcgtaggt atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg
    30961 tcatggtaat tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc
    31021 caaaaaaaag agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc
    31081 gagttacacc gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac
    31141 gggccaatag cgatattggc cattttttta gcgcaacatt tgcggcaaat tcccttctcc
    31201 atacaggtgt agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt
    31261 atcccgccag gcgagatttc gctgatgcat cagcacatcg tacagcgact gactgaacgt
    31321 attgggcagc gttatatcat tattttgcgg cggtgcattc agccaccagc tcatcatttg
    31381 ttgcagcaac cgtgcgttgg tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc
    31441 tttgtcaggg agctggagca acatggcaat ctggttttca gccccttcca gttcaatcac
    31501 gggtttatgt tgttgcttcg ccgcctgcaa tagctggtaa tcgataccgt attctgcccg
    31561 cagccccagt ttttgtgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg
    31621 ggtagaaaag agtgagggag aaatgcccat ctcctggcta atgtgctgca ggttttggag
    31681 ttgctcctcg ctaatgcgct cttccagcgc ctcgcaggca ggcaaattag caaaaggcgt
    31741 atcgctggtg gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag
    31801 caaacgggtg ggcaggggag ccatatcgtg gctacccata tgaatactgc caatcagatg
    31861 aaaatggcga ttgccgggga gggtgatatc gatggcaggc caggtgtaat gattaccgcg
    31921 cagcgcggcc caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa
    31981 atatcatgct agcgcgcggt gagggatggc gcaaatggag ttggggactt gtttaatgtg
    32041 tttgtatgat tcagtatgtt cttgcatcgc tattcacagg gaagcaacag ttaaaaacca
    32101 tgaaacaggc aacaagaaaa ccgactacac ccggagatat tcttctctat gaatatctgg
    32161 aaccgctcga tttgaaaatc aatgagttag cagagttgct gcatgttcat cgtaatcgcg
    32221 tcagtgcact gatcaataac aatcgtaaac tcaccactga gatggcattc cgtctggcga
    32281 aagtttttga taccacagtc gattttaggc taaacctcca ggcggcagtt gatctttggg
    32341 aagttgaaaa caacatgcgc acccaggaag aattgggacg gattgaaaca gtggctgaat
    32401 atttggcacg ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat
    32461 ctcgtaggcc ggataaggcg ttcacgccgc atccggcatc cgagcaccat tgcctgatgc
    32521 gacgcttacg cgtcttatca ggcctacaaa cctgtgcctg aaccgtaggc cggataaggc
    32581 gtttacgccg catccggcag tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca
    32641 tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg cgttactcac
    32701 tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa gcagtgttcc
    32761 agtaaacggc cacaaaatac cggcggcgac cggaataccg atgctgttgt agataaaggc
    32821 accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatcgcga gcgcatccgc
    32881 aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg caacatcact
    32941 gccgccaccc atcgcaatgc cgacatccgc ctgggccagc gctggcgcgt cgttaatgcc
    33001 gtcgcccacc attgccacct gacgtccttc actttgcaga cgtttgatcg cttcggcttt
    33061 accgtccggc agcactccgg caatcacttc atcaattcca gcttctttgg cgatcgcatt
    33121 ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt tatgcaggcg
    33181 ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca gggctactgc
    33241 tttcccgtca atcgccagca gcacaggcgt tgctccttgc gatgcctgag ctgaaatatc
    33301 ggcttcaatc gctttagtat cgacttgctg atcatttaac aacgcctgat tacccagcaa
    33361 taacgcatga ccttctgctt caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt
    33421 gacctgcggt agctgcatat cgcctgcttt atcaaggatc gctcgtgcca atggatggct
    33481 ggaaccttgc tccagcgccg ccgccagacg cagcgcctgc gcttcgtcaa catcagcaaa
    33541 tgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt tatcgaacac
    33601 gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa
    33661 ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagtc ccagcgcaca
    33721 cgggcaggcg ataatcagca ccgtagtggc aatcaccagg gtatagacaa tctgcggtgc
    33781 cggaccaaag aaataccaga ttgccgcact gacaagcgca ataaccacta ctaccggcac
    33841 aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc tctgggcctg
    33901 gcgcaccatg cgaatgatcc ttgatagcgt ggtatggctg ccaaccgcac tggcacgaaa
    33961 cagcacactg ccgtcctgta ccactgtccc ggcatggacg ctatcgcctt cgcctttttg
    34021 ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc cctgggtaat
    34081 ctcgccatct accggcacgc gatcgccggt cgtcaggcgc agcaacatac ctggctgcac
    34141 ttctgccaga ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg tcggtggggt
    34201 taaatcgagt aatttctcca gtgccttaga agaacgctga cgtgcgcgtg cttccagcat
    34261 atggccgaga ttgatcagac cgataatcat cgcactggct tcatagtaaa gatgtcgcgc
    34321 ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc acgccacgcc
    34381 cgttcccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc
    34441 actgcggtaa aaatggccgc cggcgaaaac catcaccgcc agggttatca ggccgataac
    34501 caaccacagg ctgcggttgt cggcggtgac catcatgtta tcgccgatca tcccccagac
    34561 catcaccggg atacccaccg ccagtgcgac aattgcctgc cagcggaagc gcttcatcgt
    34621 agcgacggcg gtttcttgct ggcgttcgcg gcgtttcgcg tcatcttcaa tcgcttccgc
    34681 gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac tgcccatcac
    34741 cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta cgctttgcag
    34801 cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca gcaactgctg
    34861 gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc
    34921 cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag atgcgtcata
    34981 acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc
    35041 ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcaagac tttctttcac
    35101 gcgtttaacg cagtgaccgc aggacaggcc gtccaggttc aggtcgatag tttgtgacat
    35161 aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct tccagaaagg
    35221 ggaaggtcaa gaaattaata aaccaggcgg gtaaaagtcc gtaaagatta aaaaatcggc
    35281 tcgatttgca tcaggattag acatttattt ctttgttttc ctgtagttaa gttgcgggtg
    35341 ctaagttaaa tcaggatgcc tgaaaatcgg cacaggggtg aggaattacc tcccgcatct
    35401 ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc agtggatcag
    35461 gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgattacat tccctttctg
    35521 gcgaatgtac caggtcaact ggcggcagtg gctatcgtga cctgcgatgg caacgtctat
    35581 agtgcgggtg acagtgatta ccgctttgca ctggaatcca tctctaaagt ctgtacgtta
    35641 gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaaattgg cgctgacccg
    35701 accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa accgctttcg
    35761 ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga aaacgccgaa
    35821 caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagca ggtagcgctc
    35881 tctgacgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc cattgcctgg
    35941 ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga cgtgtacacc
    36001 cgtcagtgct ctacgctcat caatactgtt gaactggcaa cgcttggcgc gacgctggcg
    36061 gcgggtggtg tgaatccgtt gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac
    36121 attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc
    36181 gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg
    36241 atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaatagtgt tcgcggtcaa
    36301 aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg ctgatcatga
    36361 tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc gtttccatcg
    36421 gtattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct gcgttgctaa
    36481 tggaagcctc gacctggggc gcctttgctt ttggcggtat tgtggcgatg ttttccggtt
    36541 atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggtattatc gacttttttc
    36601 gtcgcggatt aggcaacggc gtcttttcgc tagcgctctc gttgctatac ctgttgacgc
    36661 tggcggtgag catcgccatg gttgcccgtg cttttggcgc ttatgccgtg cagtttttgc
    36721 atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc attgcggtga
    36781 tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg atcctcgtcg
    36841 gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg
    36901 cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt attgggataa
    36961 ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa gtgaaagatc
    37021 cgcaggtcat tatgccacgg gcgtttctgg tggcgattgg cgttaccacg ttgctttata
    37081 tctcgctggc gctggttttg cttagcgatg tatcggcatt agagttagaa aaatatgctg
    37141 ataccgccgt agcgcaggct gcttctccgc tgctcgggca tgtgggttat gtgatcgtcg
    37201 tcatcggcgc tttactggcg acggcttcag gcattaacgc gaacctgttc gccgtgttta
    37261 acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat aaatccctgt
    37321 ggcggcagag tacctggggc aacatcatcg tcgtggtgtt gataatgctg atgacggcgg
    37381 cgctaaattt agactcactc gccagcgttg ccagcgccac ctttttgatc tgctatctgg
    37441 cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg ccgattctta
    37501 tcgttggtac gttggtgatg ttgttggtga tcgttggctt tatctacagc ctgtggtccc
    37561 agggcagccg tgcgttgata tggattattg gtgcaatctt actcagcctt attgtggcaa
    37621 tggtcatgaa gcgtaataaa accgtataac acctctctgt gcgcagtact tcctgtatta
    37681 ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc
    37741 ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt
    37801 accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt gacgccgccg
    37861 atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga actgacctta
    37921 ctgcgccagg cacggcaggt gggctttaac ctggaagaga gcggcgagct ggtgaatctg
    37981 tttaacgacc cgcagcggca cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg
    38041 gagatcgaac gacacattga ggagctgcaa tccatgcgcg accagctgct ggcactggcg
    38101 aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct ctccggctgc
    38161 tgtcatcatc gggcagggtg attaagacga gacggctcgg atatgtaggg ttatcccttc
    38221 tatcgcaatg acttcaacat acgtacctgc accgagatcc tcgctggcgc tgacaggcca
    38281 tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc agcggagatt ccagcacaaa
    38341 acgtcggcca atcagctgct gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc
    38401 gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg tcaggatggc
    38461 aaacattacc ccttgccact cccaacccag tggcaccagc cagaccacca ggccggtaat
    38521 caccgctgcc acgccgctcc acaacaaata accatttccg cccagcatct cggctgccag
    38581 cagcaacccg ccgagactga gccagaaaat atgtggatga acgaccatta actccatcat
    38641 ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc aatcgacccc
    38701 atcaggctgc tggcctctaa tggcatcatc actactttgc tgttactgga ggaaccgatc
    38761 tgctgtaacg cttcggtgta tttttgcgct acgaagtagt tcaccgcctg aatatcaccg
    38821 gaggcgatag cttcagacac cattttggtg gcgcgggctt ctgcttcggc ggaacgttca
    38881 cgcgcttcag cctgtaaaaa cgccgactga cgttcgcctt ccgctttcag gatttgcgac
    38941 tgtttttcac cttcggcttt gaggatttcc gcctgacgga tcccttccgc ttcaagaatg
    39001 taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat aagctctgcc
    39061 ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg gttggtggct
    39121 tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag catttcgtca
    39181 agttccattg aacccaacac ggtacggatg ttagtcatgg tcaggttgat gatcgccagc
    39241 tccagattgc tgacttcata ggcagcgcgt ggcgcgtcaa tcacctgaat aaaacagact
    39301 gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg gatatcgagc
    39361 acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac caccagactg
    39421 agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc
    39481 tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg
    39541 ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca aaattgctgc
    39601 ttgattaaac aaattatacc tgattactga aagagagttc ccccttattc ctgcgaagga
    39661 taaactgttt ttagtaaaaa tcagaaaaag ggaacagcga tgcaggaaaa tagtcctttg
    39721 cttcagctac aaaacgtagg atatctggcg ggtgatacga agattcttaa taacatcaat
    39781 ttttcgctgc gtgctggcga atttaagtta attaccggtc cttctggttg tggcaaaagt
    39841 acgctgctaa aaatagttgc ttcattgatc agcccaacca gcggaacgtt actgtttgaa
    39901 ggtgaggatg tcagcacact aaaaccagaa atctaccgcc agcaagtctc ttactgcgcc
    39961 cagacgccga cgctgtttgg cgatacggta tacgataatc tgatctttcc ctggcagatc
    40021 cgtgaccagc agcctgaccc ggccattttt ctcgattttc tcgaacgctt cgctttgccg
    40081 gacagcattt tgacgaagaa tatcgccgag ctatctggtg gtgaaaaaca acgcatctca
    40141 ttgattcgta acctacaatt tatgccgaag gttttattgc tggatgaaat aaccagtgcg
    40201 ctggatgaaa gtaataaaca taacgtcaat gagatgatcc atcgttatgt gcgcgagcaa
    40261 aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc ggataaagtg
    40321 attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact cgcataatat
    40381 tactaacgaa tcattagcac tggcattaat gctggtggtg gtggcaatct taattagcca
    40441 taaagaaaaa ctggcgctgg agaaagatat tctctggagc gtcgggcgag cgataattca
    40501 gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg ccagcctgac
    40561 attattgatg gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa
    40621 atatattgct aaagctttta tctcatcgtt tattgccatt acggtcgggg cgggaattac
    40681 cctggcggtg ctgattctct cagggtcgat tgaatttatc ccgatgcagg tgatccctgt
    40741 cgccgggatg attgccggta acgccatggt agcggtgggg ttgtgttaca acaatttagg
    40801 gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg gcgcgacgcc
    40861 gaagcaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa ttccgacggt
    40921 cgattcagca aaaacggttg gcttagtgag tttaccagga atgatgtccg ggctgatatt
    40981 tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct ttatgctgct
    41041 ctcaaccgcc agcttgtcga ccattattgc ctgctattta acctatcgta agttttataa
    41101 ttcgcgccac cagttggtgg tgactcaatt gaagaagaaa tgatgcgatg ccggatgcgc
    41161 agcatcgcat ccggcgttgt ggttcatgtg ccgaacaacg gaatatcaat acaacaatgc
    41221 atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagcg cagcgaggat
    41281 ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac gcagatgccc
    41341 gaacagcaac tccagcgcct cttcattgcg cccaacctga tgcagttgca gcgccagttg
    41401 cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact gttgaatttc
    41461 cggcttatca gccgcctgct tcagcagttc gatttgcgct accagcccct ggtagcgggt
    41521 gtcctgatcc tgcaacggaa tggttttcag caccgcttcc gcatcttcag aacggttcag
    41581 cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg acaactgcca
    41641 ggcgtctttc agcaatggca gggcatcggt gtaattgcct tcctgcatca gttgcatcgc
    41701 ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc
    41761 ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga acagatacac
    41821 ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt cgcagtccag
    41881 cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga tcggggttaa
    41941 ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag tggtcatcga
    42001 ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt ctacggacat
    42061 ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc gcgcttcaac
    42121 tcacccctgc aatattttgt ccatcacgcg cccaggcagc aggcgctcga gcaccattac
    42181 tgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa taaaagcatg
    42241 gcgcactttg tcgaccaccg cttccggtcc caacgtaaag cgggcggcga tgccgggatt
    42301 ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg
    42361 accgggttcg atcaggctga ctttaattcc gctgtggcgt agctccatgc gcagcgcgtc
    42421 tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg gcgtggagat
    42481 taatcccatc accgatgatg tcatcacaat acgcccttca ccgtgcggta acatcgcggg
    42541 taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat
    42601 ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat tgttaaagat
    42661 cccatacaga caattatcgg tcagggcgat cacctcgtct gctgcgcgat taacactttc
    42721 tggtgaatcc agatcgatca acacgccggt aaatcccatg ttgttcatac gctcaacatc
    42781 attcggtttt cggcaacctg ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct
    42841 ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca taactttacc
    42901 tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac tgcttcgcca
    42961 tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc
    43021 attgtggctt gaggtagacc tcttccataa aaaagggcag cagcggaaca tcaaactctt
    43081 tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca tagtttgcag
    43141 gcagacgtat ttgcattaac aatggttcag cgttggcggc tttgacatcc tgcaaaatct
    43201 ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg caaaccatca ttgcgcccag
    43261 ttcaaccagc acccaacgcg gctgatgctg tttgagcaga gccggaaggc gcgccagccc
    43321 ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac tctgccactt
    43381 atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatatccgg cgctcaggct
    43441 atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa
    43501 caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc
    43561 ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc
    43621 gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc
    43681 gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat
    43741 atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca
    43801 tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc tctgctgcgc
    43861 ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg
    43921 ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca acgagtggcg
    43981 ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggcaac
    44041 cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa ccgtgaacat
    44101 ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg cgaccgctgc
    44161 ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg
    44221 aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg gtggcctgcg
    44281 tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag caaagccgtg
    44341 agtttatggc gggcgatcgg gcgttgcgca gttcacgcga agtgccgcaa gcgtggctgg
    44401 aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc acaatgacct
    44461 ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt
    44521 atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg
    44581 ccccacgcct gatggcactg cttaacctga aaacggacga caccattgac gtgggcgatg
    44641 ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt aaccccttcc
    44701 agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga gccgtgcagc
    44761 cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag ctcgacggct
    44821 atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt ctggaacagg
    44881 acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc
    44941 ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat tactgtcgca
    45001 gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg caactgcgta
    45061 agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc ggtggggcca
    45121 taggcctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac
    45181 taccgccagc cagcctctgg ccgtggctgt gggcgcttgg caccatgacg gtcatctcgc
    45241 tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta cgcgtattac
    45301 gtaatgatgt ggtcgcgaac gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg
    45361 tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg gcggtgctgg
    45421 cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac
    45481 ttcgccgcat gacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc ctgttacgtc
    45541 agccgtggtc aacgttaagc cagctttcgg cattttcgct ctcctttatg ctgctggcac
    45601 tgctgctggt gctgcgtggc gatctgctcg accgctggca gcagcagcta ccgccagaaa
    45661 gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta aaagcgttcc
    45721 tcgcggaaca tcatatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg
    45781 cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaatcgc gaactcaatc
    45841 ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac tggccgccaa
    45901 aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg
    45961 ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca
    46021 aagtggactg ggaaagtctg cggcctaatt tctattttat tttccctgaa ggggcattag
    46081 acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga
    46141 cgcaactcaa ccgccagttc ccgaccatta gcctgttaga cattggcgcg attttaaaac
    46201 aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg gtgctggtca
    46261 ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc
    46321 aggagctggt ggtatggcgc acactcggtg cggggaaaaa actgctgcgc acgacattgt
    46381 ggtgtgagtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa
    46441 cggcactggc agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca gactggcgat
    46501 tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg
    46561 gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgtggggtga tgacaggtgt
    46621 tagattgcct gatgcgctac gcttatcagg cctacgaaga gcgtgcaata tattgatttt
    46681 gcaagattgt gtaggccgga taaggcgttt acgccgcatc tggcatgaac agtgtgcatt
    46741 ttggcaataa atatgaaacc aactgtcatg tcggtttttt taaaggccga aggaggtaaa
    46801 ccttcggcct tagctacgtt tcctcagtta ttcaataaac tgacggatca gttccagcag
    46861 cgcgattagc gccttaagaa cgatgagaac tttatcgaga aaagttttca tccgttgctc
    46921 ccttaaggga gaaagacaat cacagcttac ccttacgttg cctgtaacta tgattttctt
    46981 catgtgctag atgaggactg cctctctcca aattagagca ccagaatcag caccacctgt
    47041 atcctgacca ggacttaaaa gtacttttcc gcactgcggt aatcccttga ggcagtatca
    47101 ctactagcgt cgggattgta tcgccaacat agctgtttga atatgtaggc atcctgactt
    47161 ataattcact tgctagatga ggactgttta atttctgccc ggaacaacca agtcttgggc
    47221 ggaaaaaata aaaaaaccga ctgtcatgtc ggttttttat atctgtattc tggtgcaacc
    47281 attttgttct cacgacaatc gccgtttcag aataccgccc atcactgcgc cttaacccat
    47341 accgccactt ccgcccactc accgcgaaag acaacttttt ccgctttttt ctcaagctga
    47401 tagcgataca ttgggtcgta atattcttta agtaacggca ccagccaggc cagatgaccg
    47461 tcggtgctgc cggtggtgag ttgcgttgtc agcgccgtat ccagctgggc agcgagttcg
    47521 ttatagcgct gtaaccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg
    47581 caatattcct gccagccctg ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc
    47641 aagaaatact cttcattcag gcgttcaaga cggatctcaa acggatcttc taccaccgca
    47701 atcgccgcct gagtcattcg ctcgcgcaga cattccggca ggtgattcga accgatcatc
    47761 cggctttcat cttccagcac ccacaggcgc aaatcctgac gggcgtcggt ttttagcatt
    47821 tcggcagcca gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca
    47881 aacgccgaac cgcgatgacg cgccagccct tccagatcaa caccgtttgg ctgttgttgc
    47941 actaatagcg ttttaccgct gccggtacaa ccgccaatca gcactatcgg tttttgtgcc
    48001 agttcaatgg tcgcctgaat cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc
    48061 ggataatcaa tccccgcttc atgcagccag cgttgcacaa tatgtgaacg ttgaccgcca
    48121 cgggcgcagc agagaatacc ttgcggattt tgcaggcacg ctgcccgcca ggcgtacata
    48181 cgctgctgac gaatttcacc cgccaccagt ttatgcccca gcgccagcgc tgcgtctgag
    48241 ccttgctgtt tatagcaggt gccaacggcg gcgcgttcat cgttattcat taacggcaga
    48301 ttgatagcgg cgggcattgc gccttgctca aactcgatag gggcgcgaac atcaataatg
    48361 ggcgtatcag caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt
    48421 aaaagtgaac ctcaaatcag cttgcgcgct attttacgcg ccaacgcgca aggaaacttg
    48481 atttttaact gcgtgggttg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgtgtt
    48541 gggtaaacaa ggtcacaata tcttctaccg ctttgccgct gccaaatttg cgccatgcca
    48601 gactcaatgg cgaaggaggg cgcatcgttg ggattacccg gctgaccagt tgttgattat
    48661 cgatcattga ctggcaaagt gattttggca aaaaaccaat gccaacaccc gccagatggg
    48721 cggcgatttt cgtttccata tcgggaacaa taatctcttt ttgccccggc aatcgccagg
    48781 cgacgcgttt ggttaaggtg cgggcgctgt cttcaatatt gaccgccgga aagcgacgca
    48841 actgcgcttc tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa
    48901 atcgccattg caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcagttc
    48961 ccgtgacccc gatagccagc gaaaaacctt cgtacaatag cgagtcccag acgcccatat
    49021 agatttgtcg ggagatgtga aactgggtaa aggggtaacg ctcattcagc cacgccagca
    49081 actgggcgac ggcctggggg ttatagagca ggttgttgat gacaatattc acctggcgtt
    49141 ccacgccatc attcacctgt tgcagctcgc tgggcatact ttccagccag ctcagccagt
    49201 ctctggcctg ggaaagtaga tgctcgccag ccgctgtcaa cgttacgcta cgagtcgtac
    49261 ggaaaaacag cgctactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg
    49321 ccgtggtttt acataatcgt tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa
    49381 tgaaagtccg caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag
    49441 ttgcgtgatt ttcttatcct ctgatttatc agtattttta catgataatc ctgttcaatt
    49501 tgtggaccaa atctagtttt ggaaaaacat tccaactttt gtattgatgt tgttctctta
    49561 aggttttaga ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat
    49621 tatgttacaa ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta
    49681 gtgcttatgg cgacgtaatc gaaacgcagc aacgggattt tttccatatt aacaatggcc
    49741 tggtggagcg ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta
    49801 tcagcattaa ccgcgcgcaa ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc
    49861 atccgctggg cactcaggcc tttatcccaa tgaaaggtga agtgtttgtg gtggtcgtgg
    49921 cgttaggcga cgacaaacca gacctgtcaa cgctgcgggc gtttatcacc aacggtgaac
    49981 agggggtgaa ttaccatcgt aacgtctggc atcacccact tttcgcctgg cagcgcgtca
    50041 ccgattttct gaccatcgat cgcggcggca gtgacaactg tgatgttgaa agtattcctg
    50101 aacaggaact ctgttttgcg tgacgcctgc aaccgacttg cataagataa actaattgtt
    50161 cattgtttat gctcacttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg
    50221 cggcaggcca ggacaggcgg agcctgtggc acagaagggc gcacaggcgt tagagcgggg
    50281 aattgcgatt ctgcaatatc tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc
    50341 tctcaatctg gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaagcagc
    50401 ggattttgtc tatcaggaca gtcaattagg ctggtggcat ataggattag gtgtctttaa
    50461 cgtcggtgcg gcgtacatcc ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg
    50521 ccgcctgatg ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc
    50581 ggtattaatt ggtcagttag agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag
    50641 tcgtctgccg ctgcatgctt ccggtgcggg caaagcgctg ctttatccgc tggcggaaga
    50701 ggagttgatg agcatcattc tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt
    50761 ggatatgccc accttgctga aggacctgga acaagcgcgt gaactgggct ataccgtaga
    50821 taaagaagag catgttgtag gtctgaattg catagcttca gcaatttacg atgatgtcgg
    50881 tagcgttgtt gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt
    50941 tgtcagtcag ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact
    51001 gaaagcacat ccatagtgtc tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa
    51061 tttaaaagtt ggaaaatttt tccaattaat agaggtagga ataaaatggc aaaaatgaga
    51121 gccgttgacg cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt
    51181 ccgggagctg caattaatcc gttctactca gcgatgcgta agcacggcgg tattcgtcac
    51241 attctggcgc gtcatgtgga aggtgtttcg cacatggcgg aaggttatac ccgcgcaacg
    51301 gcagggaata tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc
    51361 gcgctcaatt ccgcttctgc tgattccatt cctattctgt gcattaccgg ccaggcaccg
    51421 cgcgcccgtc tgcataaaga agattttcag gccgtagata ttgaagcaat tgctaaaccg
    51481 gtcagcaaaa tggcggttac agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag
    51541 gcatttcacc tgatgcgttc tggtcgtccg ggtccggtac tggtggattt accgttcgac
    51601 gttcaggttg cggaaatcga gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa
    51661 cctgctgcca gccgtatgca gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt
    51721 ccggtgattg ttgccggggg cggggtaatc aatgctgatg cagctgcact gttacaacag
    51781 tttgctgaac tgaccagcgt tccggtgatc ccaacgctga tgggctgggg ctgtatcccg
    51841 gacgatcatg aactgatggc cgggatggtc ggtctgcaaa ccgcgcatcg ttacggtaac
    51901 gcaacgttgc tggcgtccga catggtgttt ggtatcggta accgttttgc taaccgtcat
    51961 accggttcgg tagagaaata caccgaaggg cgcaaaatcg ttcatattga tattgagccg
    52021 acgcaaattg gtcgcgtgct gtgtccggat ctcggcattg tctctgatgc taaagcggcg
    52081 ctgacactgc tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt
    52141 aaagaatggg tcgccgactg ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc
    52201 gacaacgtgc cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc
    52261 gatgtttgtt atgtcaccac tattggtctg tcacaaatcg ctgcggcaca aatgctgcat
    52321 gtctttaaag accgccactg gatcaactgt ggtcaggctg gtcc
//
LOCUS       DAJTYI010000012        49857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYI010000012 DAJTYI010000000
VERSION     DAJTYI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..255
                     /locus_tag="OO564_000709"
     CDS             <1..255
                     /locus_tag="OO564_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6651953.1"
                     /translation="ELQILNAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGW
                     IIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIKDI"
     gene            366..1289
                     /locus_tag="OO564_000710"
     CDS             366..1289
                     /locus_tag="OO564_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6651954.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1293..2111)
                     /gene="nlpA"
                     /locus_tag="OO564_000711"
     CDS             complement(1293..2111)
                     /gene="nlpA"
                     /locus_tag="OO564_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6651955.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2333..2626
                     /locus_tag="OO564_000712"
     CDS             2333..2626
                     /locus_tag="OO564_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651956.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2667..3857)
                     /gene="nepI"
                     /locus_tag="OO564_000713"
     CDS             complement(2667..3857)
                     /gene="nepI"
                     /locus_tag="OO564_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6651957.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4068..4520)
                     /locus_tag="OO564_000714"
     CDS             complement(4068..4520)
                     /locus_tag="OO564_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6651958.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4573..5907)
                     /gene="adeQ"
                     /locus_tag="OO564_000715"
                     /gene_synonym="yicO"
     CDS             complement(4573..5907)
                     /gene="adeQ"
                     /locus_tag="OO564_000715"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6651959.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6082..7848
                     /gene="adeD"
                     /locus_tag="OO564_000716"
     CDS             6082..7848
                     /gene="adeD"
                     /locus_tag="OO564_000716"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6651960.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7894..9285)
                     /gene="uhpT"
                     /locus_tag="OO564_000717"
     CDS             complement(7894..9285)
                     /gene="uhpT"
                     /locus_tag="OO564_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6651961.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9423..10742)
                     /gene="uhpC"
                     /locus_tag="OO564_000718"
     CDS             complement(9423..10742)
                     /gene="uhpC"
                     /locus_tag="OO564_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6651962.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10752..12254)
                     /gene="uhpB"
                     /locus_tag="OO564_000719"
     CDS             complement(10752..12254)
                     /gene="uhpB"
                     /locus_tag="OO564_000719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6651963.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12254..12844)
                     /gene="uhpA"
                     /locus_tag="OO564_000720"
     CDS             complement(12254..12844)
                     /gene="uhpA"
                     /locus_tag="OO564_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6651964.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12919..13209)
                     /gene="ilvN"
                     /locus_tag="OO564_000721"
     CDS             complement(12919..13209)
                     /gene="ilvN"
                     /locus_tag="OO564_000721"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6651965.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13213..14901)
                     /gene="ilvB"
                     /locus_tag="OO564_000722"
     CDS             complement(13213..14901)
                     /gene="ilvB"
                     /locus_tag="OO564_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6651966.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15007..15105)
                     /gene="ivbL"
                     /locus_tag="OO564_000723"
     CDS             complement(15007..15105)
                     /gene="ivbL"
                     /locus_tag="OO564_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6651967.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15670..15759
                     /gene="tisB"
                     /locus_tag="OO564_000724"
     CDS             15670..15759
                     /gene="tisB"
                     /locus_tag="OO564_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6651968.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16039..17223
                     /gene="emrD"
                     /locus_tag="OO564_000725"
     CDS             16039..17223
                     /gene="emrD"
                     /locus_tag="OO564_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6651969.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17231..17728)
                     /locus_tag="OO564_000726"
     CDS             complement(17231..17728)
                     /locus_tag="OO564_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6651970.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17725..18087)
                     /locus_tag="OO564_000727"
     CDS             complement(17725..18087)
                     /locus_tag="OO564_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6651971.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18077..18424)
                     /locus_tag="OO564_000728"
     CDS             complement(18077..18424)
                     /locus_tag="OO564_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6651972.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18533..18946
                     /locus_tag="OO564_000729"
     CDS             18533..18946
                     /locus_tag="OO564_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651973.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(18993..20376)
                     /locus_tag="OO564_000730"
                     /pseudo
     CDS             complement(18993..20376)
                     /locus_tag="OO564_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20411..22126)
                     /locus_tag="OO564_000731"
                     /pseudo
     CDS             complement(20411..22126)
                     /locus_tag="OO564_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22293..23186
                     /locus_tag="OO564_000732"
     CDS             22293..23186
                     /locus_tag="OO564_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6651974.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23183..23997)
                     /locus_tag="OO564_000733"
                     /pseudo
     CDS             complement(23183..23997)
                     /locus_tag="OO564_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23997..25613)
                     /locus_tag="OO564_000734"
                     /pseudo
     CDS             complement(23997..25613)
                     /locus_tag="OO564_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            25908..26582
                     /locus_tag="OO564_000735"
     CDS             25908..26582
                     /locus_tag="OO564_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6651975.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26621..28282)
                     /locus_tag="OO564_000736"
     CDS             complement(26621..28282)
                     /locus_tag="OO564_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6651976.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28478..28906)
                     /gene="ibpB"
                     /locus_tag="OO564_000737"
     CDS             complement(28478..28906)
                     /gene="ibpB"
                     /locus_tag="OO564_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6651977.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29018..29431)
                     /gene="ibpA"
                     /locus_tag="OO564_000738"
     CDS             complement(29018..29431)
                     /gene="ibpA"
                     /locus_tag="OO564_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6651978.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29425..29497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29736..30068
                     /locus_tag="OO564_000739"
     CDS             29736..30068
                     /locus_tag="OO564_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6651979.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30070..31284)
                     /locus_tag="OO564_000740"
     CDS             complement(30070..31284)
                     /locus_tag="OO564_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6651980.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31385..32403
                     /locus_tag="OO564_000741"
                     /pseudo
     CDS             31385..32403
                     /locus_tag="OO564_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32364..33701)
                     /locus_tag="OO564_000742"
     CDS             complement(32364..33701)
                     /locus_tag="OO564_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6651981.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33776..34924)
                     /gene="dgoD"
                     /locus_tag="OO564_000743"
                     /pseudo
     CDS             complement(33776..34924)
                     /gene="dgoD"
                     /locus_tag="OO564_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(34921..35538)
                     /gene="dgoA"
                     /locus_tag="OO564_000744"
     CDS             complement(34921..35538)
                     /gene="dgoA"
                     /locus_tag="OO564_000744"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6651982.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35522..36400)
                     /gene="dgoK"
                     /locus_tag="OO564_000745"
     CDS             complement(35522..36400)
                     /gene="dgoK"
                     /locus_tag="OO564_000745"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6651983.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36397..37086)
                     /gene="dgoR"
                     /locus_tag="OO564_000746"
     CDS             complement(36397..37086)
                     /gene="dgoR"
                     /locus_tag="OO564_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6651984.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37364..38020
                     /locus_tag="OO564_000747"
     CDS             37364..38020
                     /locus_tag="OO564_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651985.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38066..38878)
                     /gene="yidA"
                     /locus_tag="OO564_000748"
     CDS             complement(38066..38878)
                     /gene="yidA"
                     /locus_tag="OO564_000748"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6651986.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(38992..39390)
                     /locus_tag="OO564_000749"
     CDS             complement(38992..39390)
                     /locus_tag="OO564_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6651987.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39630..42044)
                     /gene="gyrB"
                     /locus_tag="OO564_000750"
     CDS             complement(39630..42044)
                     /gene="gyrB"
                     /locus_tag="OO564_000750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6651988.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42073..43146)
                     /gene="recF"
                     /locus_tag="OO564_000751"
     CDS             complement(42073..43146)
                     /gene="recF"
                     /locus_tag="OO564_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6651989.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43146..44246)
                     /gene="dnaN"
                     /locus_tag="OO564_000752"
     CDS             complement(43146..44246)
                     /gene="dnaN"
                     /locus_tag="OO564_000752"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6651990.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44251..45654)
                     /gene="dnaA"
                     /locus_tag="OO564_000753"
     CDS             complement(44251..45654)
                     /gene="dnaA"
                     /locus_tag="OO564_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6651991.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            45951..46031
                     /locus_tag="OO564_000754"
     CDS             45951..46031
                     /locus_tag="OO564_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6651992.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46261..46401
                     /gene="rpmH"
                     /locus_tag="OO564_000755"
     CDS             46261..46401
                     /gene="rpmH"
                     /locus_tag="OO564_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6651993.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46418..46777
                     /gene="rnpA"
                     /locus_tag="OO564_000756"
     CDS             46418..46777
                     /gene="rnpA"
                     /locus_tag="OO564_000756"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6651994.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46741..46998
                     /gene="yidD"
                     /locus_tag="OO564_000757"
     CDS             46741..46998
                     /gene="yidD"
                     /locus_tag="OO564_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6651995.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47001..48647
                     /gene="yidC"
                     /locus_tag="OO564_000758"
     CDS             47001..48647
                     /gene="yidC"
                     /locus_tag="OO564_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6651996.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48753..>49857
                     /gene="mnmE"
                     /locus_tag="OO564_000759"
     CDS             48753..>49857
                     /gene="mnmE"
                     /locus_tag="OO564_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6651997.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVL"
BASE COUNT        12312 a        13417 c        12560 g        11568 t
ORIGIN      
        1 gaattgcaaa ttctaaatgc catcttcctt ggtattctct gtggtatcgg catgctttat
       61 tttcaggact tgatgcctga aaaaataggc tctgcgacaa cgttatatgc aaatacttca
      121 cgcgtcggct ggattatcgc cggctctgtt gacggaatta tggttgaaat ctggagctac
      181 catgcgttgt tctggctggc gatagggatg ttgggtattg cgatgatttg cctgctgttt
      241 attaaagata tttagctcac cagtctgggc taagaaaaat cggcgtggcg caaacagagc
      301 catcctcgcc atttttgcta tcatgcctgc atacataaac gacaaaacag tatgcagagg
      361 gaaaaatggg ttccaccaga aaggggatgc tgaacgttct gattgccgcc gtgttgtggg
      421 gaagttcagg ggtctgtgcg caatacatca tggagcaaag ccagatgtcg tcgcagtttt
      481 tgactatgac gcgtttgata ttcgccggtt tgattctgct gacgctgtca tttgttcatg
      541 gcgataaaat cttttctatt attaacaatc acaaagatgc cattagtctg ctgatttttt
      601 ccgtggttgg cgcgctaacc gtacagctca cttttttgct aaccatcgaa aaatcgaacg
      661 cagccacggc aacggtgctg caattcctct caccgacgat tatcgtcgcc tggttctcac
      721 tggtacgtaa atcgcgcccg ggcattctgg ttttctgcgc tattttgaca tcgctggtcg
      781 ggactttttt attggtgaca cacggtaatc cgacgtcatt atcgatctct cctgccgcgt
      841 tattctgggg cattgcctcg gcatttgctg ctgcattcta taccacctat ccctcaacgc
      901 tgattgcccg ctatggcacg ttgcctgtcg tcggctggag tatgctgatc ggcggtctga
      961 ttctattgcc tttttatgcc agacaaggga caaactttgt cgttaacggc agtttgattt
     1021 tggcgttttt ttatttgatc gtcattggta cgtccctgac atttagtctg tacctgaaag
     1081 gagcacaatt aattggcggt ccaaaagcca gcattttgag ctgtgcagaa ccattaagta
     1141 gcgcgctact ctctttgctg ttgctgggga tcacgttcac attaccggac tggctgggaa
     1201 cgctgctgat tctgtcatcg gtaattttga tttcaatgga ttcccgtcgc cgcgccagaa
     1261 aaataaatcg tccggcgcgg gatgagtgag aattaccagc caggcaccgc gccaccgtta
     1321 aagatggttt ctgccgcttt agcgacttcg gatgattgat aagattgcag aaattccttc
     1381 acgttttctg cattcttatt atcttcccgt gccaccaaaa tattcacata cggcgaattt
     1441 ttatcttcaa taaatacact gtcgtgcacc ggagaaagcc cggtctgctg aatgtaagtg
     1501 gtgctgataa tcgccacatc aactttggga tcatccagta cgcgcgggag ctgcgccccc
     1561 tcgagttcca taatctgcaa atgacgagga ttatcggtta tatccagcgc ggtaggtaat
     1621 aacccttttc cctctttcag agtaattagc ttctcttttt gcaacagtaa aagcgcacga
     1681 ccaaggttgg tcgggtcgtt ggggatcgcc actgtcgcgc cttcttttat ttgcgccacg
     1741 gttttgattt ttttggaata acccgccatg gggaagacaa acgtatttcc caccgctacc
     1801 agtttataac catgcgcctg attatcttgt tcaagaaagg gtcgatgctg gaagacgttc
     1861 gcatcaagtt cgccatgatt tgtcgcatcg ttcggtagca acgaaccgct aaatcctacc
     1921 agttgaacat cgaggccata tttctctttt gccacctttt tagcgacttc cgcgacatct
     1981 tgttctgcgc catttataac gccaacttta atgtgttttg catcgctgct actctggtcg
     2041 caacctgcca gcagaattcc ggccagcaat aatgcggccc ctgtccgtag atgatgtgtt
     2101 gtcagtttca tttttttatc cttttattaa tgattacctg actatagcgg taaggtcgct
     2161 gcggtttaaa aaacgaaacg ctatcgataa gaataaaaag gaataaaagt ggaatataaa
     2221 gtctggcact tcttactgac cacgcaagcg cgtttcgtac agcatgatga gagcgatgaa
     2281 agcaaactac acttatgctt cattcgatac acatttgtaa aaggttgaat ccatgaaacc
     2341 aacgacacta ctgcttattt tcactttttt cgctatgcca ggcatcgtat acgccgagtc
     2401 gccttttagc tcactgcagt ccgcaaaaga gaaaaccacg gtattacaag atttacgtaa
     2461 aatttgcacg ccacaggcgt cattatcaga tgaagcgtgg gaaaagttaa tgttgtcaga
     2521 tgaaaacaat aaacaacata tacgcgaggc catcgtggcg atggaacgta ataatcagag
     2581 caattattgg gaagcgttgg gtaaggtaga atgcccggat atgtaagaac agcaacaact
     2641 actgaccaac gcttctttac ctgatatcag gatttcttca ttttcacctt tgcagtaacc
     2701 aacaatgcag tcagcaacat caatgtgccg gacaacatca acggcgaagt cagaccaata
     2761 ttatccagcg cataaccgcc gattgccgcg ccacaggtat tagcaagctg aataaccgcc
     2821 acctgaatag acccggcttt ttctgcctga tcggccagcg agcgggtgat ccacgttgac
     2881 cagccgacgg gaaccaatgc aaaagttagc ccccagataa tcgccacgcc ggtagcaacg
     2941 attttatcgc ttccccacaa cgtcagtacc aacgcactca cagccagtat taacggcgcg
     3001 cctgctaagg ccagttttac cgaacgttta agaatgaacg acgaaagcga cgtaccaata
     3061 aagctggcga taccaaaact caacagcacc agcgttaagc catccacgcc gaatcccgcc
     3121 aggttcatat acaccgggcg aatatacgtg aagaaagcaa actgcccggc gaaagacatg
     3181 aagatggcga tcatccctgc catcacaccc ggacgttgta ataagcggaa agtattttgt
     3241 ttctgatgcg agggttcgcc tggcagtgaa ggcaatgatt tgataatcca gaaaatacac
     3301 agcacgccca tcaccgccgc cgcattaaag acattgcgcc aaccgataag ctcgcctaaa
     3361 aaactgccca acggcgcggc aatcaccagc gcaatagaaa ccgcgccgaa gatcaccgac
     3421 agcgccttcg gcaccgtacg cggcggtacc agacgcatgg tcagcgacgc cgacatcgcc
     3481 cagaacccgc ccagcgccag ccccagacag gcacgaccga ttaaaagcaa actgaatgag
     3541 ttagcaaagg aaaccagcaa gcaggagagc gtcagcaaaa cggcaaacaa aataacaacg
     3601 tagcggcggt cagtggcctg aattgtctgg gtaataaaca aactggcaaa cattgccaca
     3661 aaggcggtca cggtcaccga ttgcccggca accccttccg aaatgcctaa atcctgggcc
     3721 attggcgtca acaaactgac gggcaaaaac tcaacgataa tcagacaggc gacacaaaac
     3781 gccaccgaga aaacggctga ccagttcggt cgggtgatgg catccgcgcc gcggttttcg
     3841 gcaataaatt cactcatggt gttacccgtg gcatgtttca ggggaaaagc cgaccacttt
     3901 aacattgaaa tgtgtgacgc atttaacgtt tttgcaactt ttacggtgtt gcttgagctg
     3961 gagttaagat cgaaacggaa cagggcaaag attaccggat gcggcgtgaa cgtcttatcc
     4021 acccgacagg tcaggcgcgg tctgtaggct gataagacga aacggtttca atgcaaccag
     4081 tgaacgccct cggcaggctg aaagaacgcg ttatagcgca tctgaaaagc attaatatcc
     4141 tgcatttcag gttcggtttc acgcagaaaa cccagcgcca gcctgacctg agcatcggta
     4201 atcggcgcgg aaaggcgggc tttttgtaaa atccggtgcc attgcagcag gttctgttcg
     4261 ctatcatcga caatgacaat ttgccagata agcaacacct gcgcggcgtt ttgcaggtgc
     4321 gactcatccg ggcgatgcaa ataacgtaaa aattcgtcac cggctgactt tcccatttga
     4381 tcgaccatcg actccacggg tacgacatcg atattcagcc gatcgctgag acggcgaatc
     4441 gcttttctgg ccccggatgt cagcacccga aaaccaacca caaacactac cgccagcgtg
     4501 gcgagcatta tccagatcat gagttctcct gcgtaatcag aactcatgat atctggaaac
     4561 cctcgccagt tactaatcca ccagaataat cttcaatgcg aacagagctg cgaccaccac
     4621 cacacacagg ttcagatcgc gccagcgccc ggtgcacact ttcatgatgc agtacgacat
     4681 aaagccgagt gcaatccctt cggtgatcga gaaagtaaag ggcatcatca ccgtggtaat
     4741 aaacgcaggc accgattcgg taaaatcatc ccagttaacg cgcgccaggc tcgaagtcat
     4801 cagcacgcca acaaagatta acgctccagc ggttgcgtaa ggaggaacca tcgccaccag
     4861 cggtgagaag aacataacta acaggaacat aacgccgacc acgacagccg ttagccccgt
     4921 gcgaccaccg actgccacac cagaagtact ttcaatatag gcagtaacag acgaggtacc
     4981 gataaatgcg cctgccaccg aactgacgct atcaacatac agcgccttat tcatattggg
     5041 gaatttaccg ttgctatcta ttaagcccgc tttatcagtt acaccaatta atgttcctga
     5101 tgaatcaaat aggttgatca gcataaagga gaaaatgata ccggcgagtt caagtgttaa
     5161 cgcgccgctc aaatctactt caccaatgac gccgctaata tcaggcggaa tggaatagac
     5221 gccgctaaaa tgaacatcac cgaaaaataa tccacagcaa gacgtcacca caatagaaac
     5281 cagcaccgcg gcatgaaaat gacgtgatga caacacggtt ataataaaaa accctaaaat
     5341 acctaacaac acgccgtgag aacttaaatc gccaatcatc accagcgtgt ctttattggc
     5401 gacaataaca ccagtgtttt ttaatcccat taaggcaata aataatccga ttccactggt
     5461 gataccaata cgtaaactta atggaatgtt ggagatcatc cagtaccgga tacgaaacag
     5521 cgtgagcaaa aatagcccaa ttgcgcccca gaatatcgcg cccatcccgg tctgccagga
     5581 gatccccatc gcccccacga ccacgaacgc aaagaaggcg ttcagcccca ttgccggagc
     5641 cagcaccacg ggtaagttag caaatatccc catcgcaata ctgccgatac cggcaatcaa
     5701 acaggtggta acaaacacca ctttcgggtc catttgtgcc gcgccgagga tttgcgggtt
     5761 cacaaaaaca atgtacacca tggttaaaaa agtggtcatc cccgcaatca attctgtgcg
     5821 gacggtggtc ccatgctgag gtagtttaaa taatcgcgaa agcgtccctg attcattact
     5881 cacgtaatcg gtattgtcat tattcatttt tttgtccatt ggaggagatt taatcccttg
     5941 cttttgtaaa acaaatgaca acaacggttc agtgataatt atcacatttc aattgcacat
     6001 taatagatat tctttaataa tctcgcgacg tttctttatg ataaataata atcaaattga
     6061 taaaatcaaa atgagaaaaa tatgaataat tctattaacc ataaatttca tcacattagc
     6121 cgggctgaat accaggaatt gttagccgtt tcccgtggcg acgctgttgc cgattatatt
     6181 attgataatg tctctattct cgacctgatc aatgccggag aaatttccgg cccaattgtg
     6241 attaaaggac gttacattgc cggtgttggc gcagaatacg ctgatactcc ggctttgcag
     6301 cggattgatg ctcgcggcgc aacggcggtg ccagggttta ttgatgctca cctgcatatt
     6361 gaatccagca tgatgacgcc ggtcactttt gaaaccgcta ccctgccgcg cggcctgacg
     6421 accgttattt gcgaccctca tgaaatcgtc aacgtgatgg gagaagccgg attcgcctgg
     6481 tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact tacaggtcag ctcttgcgtg
     6541 cccgctctgg aaggctgcga tgttaacggt gccagtttta cccttgaaca gatgctcgcc
     6601 tggcgggacc atccgcaggt taccggcctt gcagaaatga tggactaccc tggcgtaatt
     6661 aacgggcaga atgcgctgct cgataaactc gatgcatttc gctacctgac gctggacggt
     6721 cactgtccgg gtttgggtgg caaagaactt aacgcctata ttgctgcggg tattgaaaac
     6781 tgccacgaaa gttatcagct ggaagaagga cgccggaaat tacaactcgg catgtcgttg
     6841 atgatccgcg aagggtccgc tgcccgcaat ctcaacgcac tggcaccgtt gatcaacgaa
     6901 tttaacagcc cgcaatgcat gctctgtacc gatgaccgta acccgtggga gatcgcccat
     6961 gaagggcata tcgatgcctt aattcgccgc ctgatcgaac aacacaatgt gccgctgcat
     7021 gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact ttggtctgaa tcacctcggc
     7081 ttactggcac ccggcaagca ggccgatatc gtcctgttga gcgatgcgcg taaggtcacg
     7141 gtgcagcagg tactggtgaa aggcgaaccg attgatgcgc aaaccttaca ggcggaagag
     7201 tcggcgagac tggcgctgtc tgccccgcca tatggcaata ccattgcccg ccagccagtt
     7261 tccgccagcg actttgccct gcaattcacg cccggaaaac gctatcgggt cattgacgtc
     7321 atccataacg aattgattac gcactcccgc tccagcgtct acagcgaaaa tggttttgat
     7381 cgcgatgatg tgtgctttat tgccgtactt gagcgttacg ggcaacggct ggctccggcc
     7441 tgtggtttgc tcggcggctt tggcctgaat gaaggcgcgc tggctgcgac agtcagccat
     7501 gacagccata atattgtggt gatcggtcgc agtgccgaag agatggcgct ggcggtcaat
     7561 caggtgattc aggatggcgg cgggctgtgc gtggtacgta acggccaagt acaaagtcat
     7621 ctgccgttac ccattgccgg gctgatgagc accgacacgg cgcagtcgct ggcggaacaa
     7681 attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt tacccgatga gccgtttatt
     7741 cagatggcgt ttctttctct gccagtgatc cccgcgctaa aactaaccag tcaggggcta
     7801 tttgatggcg agaagtttgc cttcactacg ctggaagtca cggaataata aaaaaagccc
     7861 ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc actgtcaact gctgaatttt
     7921 tttctcgcgg cggattttgc gttcttccat taccgccact atcgccatca ggcagataca
     7981 accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc cagccggtaa ggccgaatac
     8041 cggcgtccca tcggcaatca ttcccagacc taacttggca aagctgtcac caatcaggta
     8101 agcaaaggtg cctttaatac catcggcagc gccaatcgct tttttaggta caaagccaac
     8161 agcagccaca ccaatcaaca attgcgggcc aaagaccagg aaacccaacg caaagagaga
     8221 agccagatag atatattcgt tactggcatg ttgatacaca ccgagcgtgg cgataatcag
     8281 cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc gccaggtcag agagccagcc
     8341 ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc gtaaagccct gaatcgccac
     8401 cgctttagag agtttcagtt cctggaacgc gtatacggtt gaccactggt cgataccaat
     8461 acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc cagatcactt tgtttttcag
     8521 cacatactca acaaagatct gccacttggt catatcggta gattctgtct ctttgtcctc
     8581 ttcgctgacc tcctcgccga acagttcttc agctttgccg aggccataag attccgggga
     8641 gtcgctaccg taacgcaggc cgataaaacc gacaatcagc gcgataatcg acgggaagat
     8701 aaacatgccg atgacatggc catcgaacag gtaatttgcc ccgaacagcg ccacacctgc
     8761 tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa ccgaggaatg tcccgcgttt
     8821 acgacgcggc gtccatttgg tgatggtgga gtaactgcac gaaccgccgg tactctggaa
     8881 aaagccgctt aaggcgtaga aggcaatcat caggaacagg ctaaccgagc cgctgcccat
     8941 actggcactg aagcccagca tacaaatggc agagaggata agcatgaacg gcaggaattg
     9001 tttggtgttt ttgccgtcgg cgtagtatga aaccaacgtt ttacccacgc cataagtgat
     9061 ggagaaaccc aggccgatca tccccagctg cgtcatgctc aacccgtagg tcgaaatcat
     9121 atcgttctgc gcgatgttaa agttcttgcg aatcaggtac atcgtcaggt agccgataaa
     9181 gaccaccagg taggattgca tgaacggttt gaaccacatt ttgcgccgca cttcgagcgg
     9241 aaggtccagg gtcggcttgc gaacctggtt taggaaagcc agcatgggtt actcctgaaa
     9301 tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc aaatcaggcc tgagatagcg
     9361 tccaagaaaa acctggaaaa tttcttagtt ttgccggata tgaagtgaaa aggtgagatg
     9421 catcacgctt cacgcggtgt ctgggcgttc aaaaagggca gtaacagcag tgcggaaatc
     9481 ccggcggcga tagcgataac cacaaaaaat ccgctccagt gccaggtatc gagcactttc
     9541 gccagcggcc aaccagcaag cgacgccccc agataagcaa acaagccgac aaaccccgtc
     9601 gccgcccctg ccgcctcttt gtgggaacac tctgccgccg ccataccaat taacatctgc
     9661 gggccaaaga caaaaaaacc aatggtgaag aagcaggttg cctgcatcac gtagctggca
     9721 aatggcatca gccacagcga gccgactgaa agcaaaattc cggcggcgaa aatcaaattc
     9781 atcggcccac ggttgccgtt aaacaatttg tccgagcccc aaccggctac cagcgcaccg
     9841 ataaatccgc ccagttcaaa catcgtcact gccgtattcg ccgtgaccag atcgacgccc
     9901 agcgtctcgg acatatacaa attgccccag tcgttgatcg ccgcccggac cacatagacc
     9961 agcacatagc aaaacgaaag cagccagata tacggattca gcaacacata tttggtgagg
    10021 atctctttac gcgtcaaccc tgccccttct tgttgttgag caatttccag cgcgtcgtgc
    10081 cgccattcac cgaccgccgg taaacctaac gcctgcgggc gatcgcgtag ccgccagcag
    10141 agaaaaatcc ccacgactat cgccatacaa ccagcaatca tcatcccggc acgccagccg
    10201 taatgcagcg cgctcgctgc catcacaatg ggaatgagtg cgccgccgac gttatgcgcc
    10261 gtgttccata atgcccacca accgccgcgc tcggtacgtg aataccaggc cgttaacaga
    10321 cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt tcagcaccca gagcacggca
    10381 aacgcccata gcgacgtcga gaagccaaac agaatgttca tgatgcccgt ggcgataagc
    10441 cctatcccca taaaataacg ggcatttgag cgatcgctga caatgccgga gacaaacttc
    10501 gacacgccgt aagtaatgta aaacagggtc gctaacaggc cgatatcgct acggctgagc
    10561 acgccgttag caaggatttc tggtaccgcg gcgttaaaac ttttccgcgt gaagtaaaac
    10621 agcgcgtaac ccagccagat ggtcagcaga atatgccgac gccagtagcg ataacgggca
    10681 tcaatttcat gtttatcagt cattaatggc gcatcggcag gcgctttcag aaacggcaac
    10741 atcatcgaac cttagacata acgttgaggt agagaaacgc tgacacgcgt gccgtgcaga
    10801 caggaaatgt gtaatgtgcc gcccagcgcc gttacgcgct cgcgcattcc ggtgaggcca
    10861 aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat cgtcttcaat caccagcatc
    10921 aaccgttcat cctgctgcca gccttgcagc gtaaccgcgc tggcatcggc atgtttcaca
    10981 atgttgttca gcccttcctg gcagacacga aacagcgtca cgcgctggtt ttcgcttaat
    11041 gctgattcat cgatacgcca ttcgagatgg ctgacaatgc cgcgcccttc cagctccatt
    11101 tcccgcatca gtgagcggat ggcctgctcc agggtgagat catccagctg gcgcggacgt
    11161 aaccgtccta acaaacggcg caccgcgtcg taaacgccca gcgacagttg ttcgatgagc
    11221 tgcccgctct gcttcacact ggcgttatct gccgccagcc gctgaacaat gcccgcctga
    11281 gtacgaatag cagtgatggt ctgaccgata tcgtcgtgca gctcacgcgc cacgtcgcgg
    11341 cgcacgctct cttcggtttc taacaaacgt tcagccagat gctgattgcg cgccagttcc
    11401 ttttgtagcg actggttaag ttcacgcaac cgctggatgc ctgcgcccag taacaaccct
    11461 gtcagacttt gcaccagcaa cgagagtaat aaatccacca gatgatcgcg ccaggtttga
    11521 ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca gcgctccttg ccagccatag
    11581 tgccaggcca gcgcgataat cggcagcgcc agacagaatg gcgtaaagcg cgacagttcg
    11641 tccggcaatc ccaactggag ccagagactg ataacaaaca gcagcaagta ccagaccaga
    11701 tgccgcccgc gccagttgat gggctgagaa accagtgacg ggccgagcgg cagccaggtg
    11761 ttattggcga gatagtgcca gaacaccaga catatcgggg ccagcgtcag accgccagtt
    11821 aaagtcagca acagcgcatt ccacgactct ttgccgtgcc aaagccaggg cagcgactgc
    11881 aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca aggtgcgcca gtcacgctga
    11941 tggcgatagc gcgagatcag cgctacgggc agtaacgtca gtaaactacc gatcatcaat
    12001 aacggaaaat gggttaaacc gaccgcctgc gttagccagt aaatcagcag ccactccgca
    12061 cccagcaata cgggccagta tccgcgcggg cattgcagca ttagccccag acgcagacca
    12121 aacggaaata acagcaccgc catatcaggg cgctcaacca gatgcaggct gatactccat
    12181 aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa taacggtaat taagcgggag
    12241 aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc cagctctacg tcgttactga
    12301 cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac cgttttcggt gacaagccca
    12361 gttcggcggc aatctctttc accgccattc cttgcgccag tttttccgcc acctggcgtt
    12421 cacgtttggt cagcgggtcc tgacgaccgg atgccagttt aatggcaata tccggcgtca
    12481 gataacagcc gcccgtggca accgtatgca ccgcagcaat gagttcatcc gggctacagc
    12541 gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg ctcaaccagc gcaggactgt
    12601 cgtgaacgga gagcataatc gtcgccatac ctttcggcag ctggcttagc agctccagac
    12661 cggagatatc gggcatggag atatcgcaaa tacacacctg cacaccgcgc cccggcagcc
    12721 ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac ctgcaaatca ggttccagcc
    12781 ccagcagctg cgcaaagccg gagcggacga tgaggtgatc gtctataagg gcaacggtga
    12841 tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac cttaacgata agcgcgatgt
    12901 tgttcaagcc ttgagcggtt actgaaaaaa caccgcgatc ttgttaaaca tcgtcggatc
    12961 ggactgatta cgctgcactt tcacgacatc ttccagctta tcgatttggc ttatcatctg
    13021 ctccagacgc tggtcgtcat tgaccagtag ccagatatgg cttttgtcgc tgtcctgaat
    13081 cggcagacaa agaatgcctt caacgttaaa agcgcggcgg gcaaaaaggc cacaaacgtg
    13141 ggtcattacg cccggatggt tgcgaacggt gagctccaga attacgttgt tatgagttgt
    13201 gttttgcatg gcttattccc ccaccatttc agtattcgcc gcacctggcg gcaccatcgg
    13261 gtaaactttt tcttcggcat caatgcgcac atggatcagc gccgggccag ggcgattgat
    13321 tatttcctgc aatgcagcct gcggatcggc ttcgttattc aaatcacagg tttcgaggcc
    13381 gaatccggcg gcaatctgca taaagttgat tttgcccgga taggtggcgg caaaaacgcc
    13441 ttgctcgtag aacagactct gttgctgatg caccagcccc agcgcttcgt tgttcatcag
    13501 aatgattttg acatccagct gattttcact ggcggtcgcc atctcctgaa tattcatcat
    13561 caggctgccg tcgccggaga aacacaacac tttgcgatcc gggttcgcca gcgccgcgcc
    13621 aatcgccgca ggcaggccaa aacccatcgt gcccagccca ccggaggtca gccactggcg
    13681 tgggcgattg agcggataag cttgcgcggt ccacatctga tgctgaccca catcggtggt
    13741 gataatcgca ttgtcatcga cacaggcggc aacggcgttg atcaggccgt aatggcttaa
    13801 tggatcgcac gctttcggga ttggacacgg gaattcgcgc tgcaaatccg ctaccaactg
    13861 gtgccactct gcacgcagtt gcgcttccac ctgcgggatc aattgcgcca gcacgtcatc
    13921 aacatccgcc tgaatcgcca catgcggctg cttgatttta cccagctctg cgcgatcgat
    13981 atcgacatga ataattttgg cattcggaca gaactgctcg gttttgccaa tcgcccggtc
    14041 atcaaaacgc gcaccgagca caatcaacaa atccgcctct tgcaaaatat agttggtact
    14101 gcgcacgccg tgcatcccca gcatacccag cgacaacgga tgcgcttttg gcagcatgcc
    14161 cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc tccgccagtt cacgcacccg
    14221 cgcaggcgca ttgatcacac caccacccag ataaagcacc gggcgtttgg cagcgttaat
    14281 cattgtagct gcgtcacgaa tgctttcttc gctaaaggag ggtgcagcgg ctttttctgc
    14341 caccgcgggc tgagcttcaa tctcaaaaac cgccgtttgt acatccttag gaatgtctat
    14401 ccacaccggg ctagggcggc ctgattgcgc aatgcggaag gcatcgctca tgacctgtgg
    14461 gagctcttcg atatgtctga ccagatagtt gtgtttggtg atggggatag agatgccgta
    14521 ggtgtccact tcctggaagg cgtcggtgcc gatcatcgag gcgggaacct gaccagtgat
    14581 gcaaatcagc gggatggagt ccagccgcgc atcagcaatg gcggtcacca ggttagtcgc
    14641 acccggcccg ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg ccattccctg
    14701 agcgataaac cccgcgccct gttcatggcg agccagaata tggcggattt gcgtgctttg
    14761 gcttaaggcg tcgtaaacag gcaggataga accgcccgga atgcccgtca caatcttaat
    14821 gccctgctgt tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga
    14881 tgttgtgccc gaacttgcca tgctccagtc cttttcttct gggccgactt tccgggaggt
    14941 gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc
    15001 cagtccctac ggcgcattgc cgacgaccac caccacacgc acgacgacca ctgcggcgga
    15061 tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta gtcattgggg accttgttgg
    15121 ttttgtgttt aacaatattt atacaagcac agctttacag gggagacaat ggaaaatttt
    15181 tcagcaaggg aaaattgagg ggttgatcac gttttgtact gaattgcaga taacaaaaaa
    15241 ccccgccgga gcgaggtttc gtcagccgcc tgcggctggt aaccgcaaag cacactgtat
    15301 tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc gcaatttcgg cacgaatttt
    15361 gacgtattta gtgcatagtt gagtatcgtt cacagtttgc gttttgtcca aatattactg
    15421 tttatttata cagtaaactt ctataatatc actgttcgca atgtgttatg cgggggccgc
    15481 atcgttaccc ggcgcactaa gtcctggctg aaacgggtgg tgccgtcagc gccttaaccc
    15541 cgcgtgagca cactgtgtta tgtcaacaag cacaacgttt ctccttgaga taccgcgtgc
    15601 acaacagctg gcaacaggca gcggaaaggt acgtcagctg gcagtgctcc tgaaccacag
    15661 gagacgcgta tgagcctggt ggatatcgcc attcttatcc tcaaactcat tgttgcagca
    15721 ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat tcagattcaa gtcgcaccaa
    15781 aggggagcgg gtaaccgctc cccttttata tttagcgtgc gggttggagt cggatgcgat
    15841 gctgacgcat cttatccgcc ctaccatccc tcccggcaac atttattgcc gcttttgttt
    15901 acatattctg ccgctaaaca attccccatt cctggcgtat atctggctaa cattcatcaa
    15961 tgtgatagat tcctctcccg catttatggg aatacgtagt gacttattct aattattttt
    16021 ataaaagcat ccgtgataat gaaaaggcaa agaaacgtca atttgttatt gatgttggta
    16081 ttactcgtgg ccgtcggtca gatggcgcaa accatttata ttccagctat tgccgatatg
    16141 gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg taatgggcgc ttatctgctg
    16201 acttacggtg tctcacagct gttttatggc ccgatatccg accgtgtcgg tcgccgacca
    16261 gtgatcctcg tcggaatgtc catttttatg ctggcaacgc tggtcgcggt cacgacctcc
    16321 agtttgacgg tattgattgc cgccagcgcg atgcagggga tgggcaccgg cgttggcggc
    16381 gtaatggcgc gtactttgcc gcgtgattta tataaacgga cacagttgcg ccatgctaac
    16441 agcctgttaa acatggggat tctcgtcagt ccgttgctcg caccgctaat cggcggtctg
    16501 ctggatacga tgtggaactg gcgcgcctgt tatctctttt tgttggttct ttgtgctggt
    16561 gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc cggtcgatgc accgcgcacg
    16621 cgcctgctta ccagttataa aacgcttttc ggtaacagcg gttttaactg ttatttgctg
    16681 atgctgattg gcggtctggc cgggattgct gcctttgaag cctgctccgg cgtgctgatg
    16741 ggcgcggtgt tagggctgag cagtatgacg gtcagtattt tgtttattct gccgattccg
    16801 gcggcatttt ttggggcatg gtttgccggg cgtcctaata aacgcttctc aacgttgatg
    16861 tggcagtcgg ttatctgctg cctgctggct ggcttgctga tgtggatccc cgactggttt
    16921 ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc tgttcttttt cggtgccggg
    16981 atgctgtttc cgctggcgac cagcggcgcg atggagccgt tccccttcct ggcgggcacg
    17041 gctggtgcgc tggtcggcgg tctgcaaaac attggttccg gcgtgctggc gtcgctctct
    17101 gcgatgttgc cgcaaaccgg tcagggcagc ctggggttgt tgatgacctt aatgggattg
    17161 ttgatcgtgc tgtgctggct gccgctggcg acgcggatgt cgcatcaggg gcagcccgtt
    17221 taagcgcacg tcaccgcagc atcgtcatca gctccatggg agaacgatgc tgctttatca
    17281 gatcacgcat cacccgcata tgcggtgcgg agtaagaata aaacgcctga tagcccgcac
    17341 aaagcacgct gtctggcgtc tcctgccgag cgtgcgggca caggcgatgc cagtcgcacg
    17401 cctggcagtc acggcgcggt tgcgggtttt cgctgccaca ccatttttgt aaggtggcgt
    17461 caaaaagctc aatcgagata cgactgatat ctgcccgcac ccaacactca aacagggcaa
    17521 ttaaaaattt cccccacagc tctgctgtca ccgaagcggc cgtcagatcg ccccggtcat
    17581 caaattcaag atggggaatg agctgtacgg agcggatgcc ccgctgtttg aaattgtgat
    17641 aaaaatcggc aggttgcaga gcatccttgt actctacaac cagcttatgc ctgtcctctt
    17701 cagcatcaat gacctgactt cctgtcatgc gactctctca ataaatacga taagttgatg
    17761 tatatgcgtt acagcaaaca gtattgcgag agataacacc gcgagggaga tcaaaaattt
    17821 gtcacggact acgtgaaatt gggaaaaatc gctattcgtg acatccatta aattgcgatt
    17881 acgggtgtag tgccagagga tcagcgccac gatggcgagg atgccaatcg aaatccagaa
    17941 taacatgccc gcctggtgcc agttgtgttt gatagccaac gccatcaacg cgccgtagcc
    18001 cagcatggta cgaaaccacg ccagtgatgt gcgctccggc tgcagccctg ggtcggcaat
    18061 gcgtctggct ttacggctat ccggcataca acaccagtcc cataacgatc accgccacaa
    18121 ccatcaatat taagctgatg attaataagc tgttggtgta gggcaggtcc tctttcagac
    18181 gcatcgcttt ttcattgcgt aaccagcgca ggtagccata cattgctaac ccgccggaaa
    18241 acagacacaa caacaacgcc agcagttcac gaatgacggg cgtggcgaaa tctggcgcaa
    18301 gttgatcaag cccaaccccc gccgccagaa atcctagtgc ggtacggatc cacgtcagaa
    18361 aagtacgctc attagccagc gagaagcggt aatcgggcgc ttctccaagg cgggaaattt
    18421 tcattgttgc tcctttgcaa tttcactaac gctctatctt tagcggtttt ttttaagaat
    18481 ttcgtccgcc cgtttcctga tatgcttttg ccatccgcta caaggaggtc agatgggtat
    18541 catagcgcaa aataaaatca gttcattggg catgctgttc ggtgccatcg ctttaatgat
    18601 ggggattatc cacttttcat tcggcccttt ttccgcacca ccgccaacgc ttgaaagcat
    18661 cgtggcggac aaaacggctg aaattaaaag aggtcttctc gccggaataa aaggcgaaaa
    18721 aattaccact gtggagaaaa aagaggacat ggatatcgat aaaatcctcg atcaaagcgg
    18781 tatcgcactg gccattgctg cgctactttg cgcatttatt ggcggtatgc gtaaggaaaa
    18841 tcgctggggc attcgcggcg cgctggtttt tggtatcggc atcgtgtgca gcattttgct
    18901 gatattcctg attttttcct tcctgaccgg cggttcgctg gtttaagcag cctgaataat
    18961 gcccgccggg tgatcatcag cgggcattat cgtcagaatt tctgtttttt ctcctccacc
    19021 ttcactcctt gcgtcggtag gccggtgtca tagtcgcgta ccactggcga atagccatct
    19081 tgtgggcgtg ggcgaaacgc ccccatccag cgcggtcgtg catctttacg ccacggacgc
    19141 agactccatt ggtaactgcg gaacggatcg cgaattttgt ccatgtaatc caataaggcg
    19201 tcatgcattt tgctgcgaac gtctgcaaaa cggatatcat cgatcaggtt atgcatttca
    19261 tttgggtcat tacggcgatc gtaaagttca tcactggtga agaggttgag caccagttta
    19321 aagtcatccg tcacccagca acgcaccgga ataaaaccgc caaagctgtc atgctcaatc
    19381 tcgtagcggt taaattccac catcacgccg cgtggctctt tcacggcaag gatattttcc
    19441 cccggcagaa tctctggttt ttcaatatct gccagcgcca tcattgtcgg cagtaaatca
    19501 atatgactga ctggcgtatc gacctgtcgc cgctccttta ctgatcagct tatgtgcgcc
    19561 catcatttca ccgtgatcgg aggtataaat gacccacgta ttttcacgtt gctctggcgt
    19621 taaggcattg atgacacgtc cgatttggtc atcaacaaag tcattgcagg caaaatagag
    19681 cggatggtga taaagcccgt catcaccgac tggcgatggc atcgcctgcg cccataagcg
    19741 gtgatgttcc ggtttgttcg ccaggtcatc ctgtgatttc tcgccaagtt cgtagtaaaa
    19801 atcagcgtat ttctctaaat actccaccgg acaggtgaac gggtgattcg gttcatcata
    19861 cgaaaccacc atcaggaagg gttcctcggc gcgcacgggc tgttgcagaa aatctaccgc
    19921 tcgattgctg atgcggtgcg cccaggtgaa ggtttcgtca atatggttcg cctgtaaatc
    19981 ttcgacgctg tttaggccat tgcgccacag gctgatctct ttttccgtca gttcgctaag
    20041 atagttcgcc ccatcaaacc agtaatcagc atcccactcc ggcggacact cgccagtgcc
    20101 gaaatagtca tgaccgtcga gatgccattt gccgatgtaa caggtgtgat agccggcatc
    20161 cttaaagtag cgccccatag tggagatgtt tttgcctggc gcgacgttgt tggtccacgg
    20221 gccggactgg ttagcgtaga taccggtgaa tagtccggcg cgtgcaggcg tacaaaccgg
    20281 tgaacaagtg taggcggaat taaagcgaat accttccgcc gccagactat caatattttg
    20341 cgtattcagc ggtttaccgc tatagcaacc gaccatattg gtggcctggg tatcggtcaa
    20401 ttggggcgtt tcataggcta tccttcgcgt cgggagtaaa ggttacggct gggtcgtgac
    20461 gatgccgcca gctgtcaaaa atcaggtaga caatcactac ggcagcgatg aaataactga
    20521 tcatcgctaa ccagcgcgta ccgtagccgc cgaattcagc cagcccgaca tagacgccaa
    20581 tcatcgcgaa caggatacca attgacgcga tcttgacgtt tttccacggt ttcatgtcca
    20641 ccgcaaacgc atctttgaag gtgaacggcg tggcgcgcgg tttgataaaa ccgatcacca
    20701 gcatcacgac cacgttgatg cagaacgtac aggccagcac atagaggaaa tggaagtcga
    20761 acttcaccag atagttgatg gtgatgtagc tgattatgcc aatccccatc gccacttttg
    20821 ccgccagcgc cgggatgcgc gggaagaaaa agcccataat gatgatggta accagcggca
    20881 cgttgtaaat gccgttgagc tgtttcatcc agctatacag cccctgcggc gcgttggcga
    20941 tccacggcgc taccaaaacc gaaacgatag cgataaagaa accaaatttg cgcccgacgg
    21001 tgaccagctg ctgcggctcg gcattctggt taatgatgcg acggtaaata cccatactga
    21061 ataatgtact ggcgctattc agaaagccgt tgaaggtact gatcaccgca ccaaataaca
    21121 ccgcgccgaa gaaacccacc agtggcactg gcagaacgtt attgaccagc gtcgggtagg
    21181 ccatgtcggc tttcggtaga tcctgataca gatgaaacgc aatcaaccct ggcagcacca
    21241 gtaccagcgg gtcgagcatt ttcagcaccg ccgttaacag cgcccccttt tgcccttccg
    21301 ccaggctttt tgacgccagc gtgcgttgca cgatgccctg atttgtacac cagtaaaagg
    21361 tgttcaccag aattaaaccg gtaaatgccg cgccaatcgg caagggatcg gtcgggccac
    21421 cgactgagtt taatttctcg gcgtgaacgg tggtaatttg ctcaatgccc tgcataaagc
    21481 tgcccttgcc catcgcgatc aggccaaata ccggcaccat caacccgccg ataaccagcc
    21541 caataccgtt gatggagtcc gccactgcca ttgcgcgcag tccgccgatc accgcataca
    21601 aaattcccgc cagaccaagc aaaattacca gtagccagat agccgcaccg tgagaaatct
    21661 gtagcgattc cccgacgtga aacaggctgt tgagcgccaa cgcgccggag tagagaacaa
    21721 tcggcagaaa gcagacgccg gtggcaatga ggaagcagaa gtcgatgata atacgcgtcg
    21781 ttttatcata acgttcctcc agaaaatcgg ggatggtggc aatgccgcgc ttcagatagc
    21841 gcggtaaaaa gattagcgcg aggaagatca gcgtcaccgc tgaagtcact tcccagccca
    21901 tcaccgacat gccgcttttg taggcctgcc cggaaagacc aaccagttgt tccgtggaaa
    21961 ggttggttaa cattaacgaa gcggcaataa ccggcgcttt tagtgaacgt ccggcaagaa
    22021 aatagccttg ttgcgatccg gtatctgttt tgcgaacctt ccactaggtg atcaccgcca
    22081 ccagcagcgt aaaaccgaca aaactcaaga tttgtaacga attcatctca tagcccttat
    22141 ttatgtttat gtcccggacg catgaatata gggagaggta aaatgtgtcg ggtcaggcag
    22201 tcagattctt aaaatacaaa cgtcgtatcc ctgaacggat tgttttctgt taagttcagg
    22261 ttgtgagcat aattcgccgg agggatatta aaatgaatgg aaaattgcaa agttcagatg
    22321 taaaaaacga aactccgtac aatattccct tattgattaa cgaaaatgtg atctccagcg
    22381 gaatttctct gatctcgctc tggcatacct acgccgacga gcattaccgg gtgatctggc
    22441 cgcgggacaa gaagaaaccg cttatcgcca actcatgggt tgcggtgtat accgtacaag
    22501 gatgtgggaa aattctttta aagaatggcg aacaaataac tctgcatggc aactgtatta
    22561 tatttttaaa gccaatggat attcactctt atcactgtga aggtttagtc ttggaacagt
    22621 actggatgga atttaccccc accagtatga tggatattcc cgttggtcag caaagcgtta
    22681 tttataatgg cgaaatttat aatcaggaac tcaccgaagt tgctgagtta ataacttcac
    22741 cagaagcaat aaaaaataat ctggcagtcg cttttctgac gaaaattatt tatcagtgga
    22801 tttgtcttat gtctgcagat ggtaaaaaag atccacaacg gcggcaaatt gaaaaattaa
    22861 ttgccacttt acatgccagt ctgcaacaac gctggagcgt agctgatatg gctgccacga
    22921 tcccctgtag cgaagcctgg ttgcgtcgtc tgtttttacg ctataccggc aagacgccga
    22981 aagaatatta cctcgatgcg cgtctggatc tggcgctatc gctattaaag caacaaggaa
    23041 actcggttgg cgaagtcgct gatacgctca acttcttcga ctcctttcat ttcagcaaag
    23101 cctttaaaca taaatttggt tatgcgccgt cagccgtgct aaagaatacg gaccagcacc
    23161 caacggatgc cagtccacac aattaacgta actctggcca gtacgctttg ttggcctcca
    23221 gcaactcatc cagaatagct ttggcgaccg aggcgctcgg taccgttttc gacaacgtaa
    23281 tcgcctgcca caggtgctga tatgaacgct gttcccaggc gtccacgacc agtttttcca
    23341 ccgccacttg ctgactcatt aaccctttct gaaactgcgg gatatcgccg accaccagtg
    23401 gttcaggccg tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc
    23461 catccggctt tcaataccga ttggcatgtc acagatgttg aggattttcg cattcgggcg
    23521 cagacgacgc gtggcttctg cgacaattgc tgccgggttg gagtagttga gcatctaggc
    23581 gtttggtgaa tatttttcca tataatccac cagttccagg acgccgccaa tggaacgcat
    23641 gccgtacgct attccgccgg gtccgcaagt ttcctgacca acaacaccgt ggcgccgtgg
    23701 gattttttca tccagttcgc gcatcgggta cttgccgacg cggatgtgcg ccataacaaa
    23761 atcaacgtcg ctgaacgcca cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc
    23821 tttttcttta aggatgactt tacaggcttc ggcaatcact tcctgccgcg cgccatcgtt
    23881 atcataaaat ttcagtgcac gaagcgggaa acggtcctga ttcgccagga gcatcaacac
    23941 gatgcctggc gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt tggtcattat
    24001 actgcctccg taatggcaac attttctgct gattgatgag aattaattaa gctatcgagc
    24061 tgttcacgca attgggatac atgcagacca atgatcacct gaatggcatc gccactacgg
    24121 aagacgccgt gcgctcccag ctttttaaag acttcgtcat ccagcgtttg tgacatgtca
    24181 tgcagtgcaa tacgtagacg cgtcgcgcaa ttgttaatgc tggagatatt gccgaccccg
    24241 cccagggctt gcaggatacc ggcagcctga tccagctctt tttttggctc tgcagcggtg
    24301 gtttagcctc gcgaggcttt gtattcggct tttgagtaga gtttcacttc cgcatcttca
    24361 cgtcccggcg tgcacatgtt gaactgcaga atcagggggc ggaaaaccac gaagtacagc
    24421 agggtaaagc acaacccaat ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac
    24481 atcgggatcc agttttgcgg taaaacctgg tcaatcagac ctccgcccat gttgcccacc
    24541 acaccaaaga gatacattac ggtcgacatt gaggccgcca gcacggcgtg taccgcaaac
    24601 agcaacggtg aaatgaacag gaaggtaaat tccagcggtt cggtaattcc caccagcatg
    24661 gcggttaagg ttgcgggaat cagcaagccc gccactttta cccgattttc cggtgcggca
    24721 gtgaagtaca tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt accgtgcagg
    24781 gcaaaaccgc cttccgggaa caacgatttc agcggctcgg cgctcagact gaactcttgc
    24841 agatgctgcg cccagtacat ctgaatgccg ccttcaacag ctgccggacc aaagataaac
    24901 ggtccgtaga tgaagtggtg taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa
    24961 acccacaccc caagcgcacc cgccgaacgc aggaacgctt gcagagattc aatccccatt
    25021 tgtacttttg gccagccgag caatgtcagc caggcacagg ggatcatcac caggaaggcg
    25081 ataatcacca cataagacgt cccctggaaa atgccgagaa aaaccggcag ttttttatcg
    25141 aacagacggt tatgcagcgc cgtcacaatg ccggaaatga taattgcgcc gataatgctg
    25201 gtatcgaggg ttttaatccc ggccatcatt gtcagaccgc tacctgccac cgcgtcctga
    25261 gtgaaatcga cgccgaagta gcttccccag gtcattccca tcgcattgat gaaatagttc
    25321 caggtcagga aactcaccat caccgccaga caagcacgtc cctgcgcttg cttagcaagg
    25381 ccaatgggta aaccgacagc aaaaatcagc ggcatattac ggaataccgt ccaaccgccc
    25441 tcttcaataa tgtgtacgat ttgcgcgaat aaactgttcg gatcggtcag tgattccccg
    25501 acaaacatcg ggttttgcag caagatggca agacccacca caatcccggc gaagggaaac
    25561 agcagcactg gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag catttccaca
    25621 tcctcttttc tcaattctgt aagtaggaag cctgattggt tatgcggcag acctggagcg
    25681 aggataggaa gatgcgatcg gcagaacatg acgacccaat gggattcgtg atcgctttca
    25741 tgcttttcgt tgggcggtag agatctacgt tttcgcgaaa aagtagacat ctgtgagcac
    25801 aggtacacag catcgaacac atctttaaaa aaaagatttt tttcaatcga ttaagcagaa
    25861 cttgtgggcg cattacccgt gcttgcaggc aaaaaagaga gctagagatg atctacaaaa
    25921 gcattgcgga gcggttaaga attcgactta attccgcgga tttcacgcta aacggccttc
    25981 ttcccggtga aaaaaagctg gcggaagagt ttgcggtatc gcggatgact atccgtaaag
    26041 ccattgacct gctggtagcg tgggggctgg tggtccgccg ccacggcagc ggcacttacc
    26101 tggtgcgcaa agatgtgctg catcaaaccg ccagcctgac cggactggtg gaggtgttaa
    26161 aacggcaggg aaaaacggtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc
    26221 cggccattgc cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg
    26281 ttcgttttgt ggaagggaaa ccgctaatgc tggaagacag ctatatgccg gtaaaactgt
    26341 tccgtaacct ttcactgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt
    26401 gcgggatttt gattggcggt aattatgaaa gcctgatgcc ggtgctcgcc gatagactgc
    26461 tggcgcggca aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat
    26521 atagcgagag cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatact
    26581 aggtggagta ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga
    26641 gcaccgccag taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg
    26701 cgtaagagag cgccgccgca ccactggttg gatgaagatt attagcaaac gccagcgccg
    26761 gaggatcggt catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta
    26821 acatccgcgc cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac
    26881 caatccagct tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca
    26941 gaccaacgac cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg
    27001 gcggcataaa ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa
    27061 tcagcggacc gcccgccagc cccagtttca acgcggccgg gaatcctggc acaaagacgg
    27121 gaatagaacc taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct
    27181 gttgcagttt ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac
    27241 gccccaccag gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt
    27301 cgaccccggc acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt
    27361 cgcgaatacg ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat
    27421 cagtgccttt cgtagacagc gaggtatcga cctcctgacc aatcaccagt tgcgcattat
    27481 gtaaatccgc tggctgcccc accagatgca gcaaatcgcc cagttggata atggtatctg
    27541 gcgaaggtac ttttagggtt tcttctcgtt tcagacgcga gcagataatt ttgtcgccgt
    27601 tgagaatcgg tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat
    27661 tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg
    27721 tctcgacatt gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa
    27781 tgccgaatgg atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg
    27841 tacccaggtc gcgcaagatc tgctgccctg cccccagcgc tggcgtattg gttaccgcac
    27901 cggagaaaat ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg
    27961 taaccagacc accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga
    28021 cgcgcaatga ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga
    28081 tcaggccaaa ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag
    28141 aaacaaaatg gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc
    28201 cgcgaaattt gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga
    28261 ccgttaatgc tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg
    28321 attatggctg aatcggtgaa gcagatttgc cttatagcgc acattatcac gggaaagaac
    28381 gtgccgaaat atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa
    28441 agccccacca gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt
    28501 cgctgatagc gatacgctgc gctgcgatgg gttctggctc attacgaatt aaatcaatat
    28561 gcagtaaacc gtttacgaag gttgcgccag agacttccat attttcagcc agcgtaaaac
    28621 tcaggctaaa tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct
    28681 gctccggcgt gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct
    28741 gacggaaacc tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt
    28801 tgtacggcgg gaagctctgg ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa
    28861 aaccgatcca ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt
    28921 ctaagaagcg agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc
    28981 atggagatgt caggccgcgc caggcggcct tagggaatta gttgatttcg atacggcgcg
    29041 gttttttcgc ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt
    29101 tagccccacg aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag
    29161 cgatgccctg gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca
    29221 ccagcagatt atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga
    29281 tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat
    29341 tactctggct ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac
    29401 ggtaaagcgg ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat
    29461 gtaagacctt ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat
    29521 cgactacgat gtaaaatggg tctggaaatg aacttttcaa gccttatcag cggacttttt
    29581 tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt aaagtgcgat
    29641 agccgccaca gtgcgacgta atgatgatgt tattttcagc aagggatcgc tataactcat
    29701 caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg ttgttagcgt
    29761 tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg tcccataccg
    29821 gggggaaaga aggaacgtat cccggcaccc gcgccagcgc caccatgatt ggcgacgatg
    29881 aaaccaactg gggctctaag tcgctggcga ttctcgatat gccgtttacc gctgtgatgg
    29941 acacgctttt actgccgtgg gatgtgttcc gcaaagacag ctcggtgcgc tcgcgcgttg
    30001 aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc agaatgcctg
    30061 ataactgatt tagcgatcgg tttcggtgat ccacagctgg ccaccttcca tccacgccag
    30121 ccattgacca tccggcgaga agaccacggc gtccgcagaa ggtggattgg cgtggttttc
    30181 ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa acaattcgat tatccagcac
    30241 aaagcccaac cattctcctg acggatgcca gttaaatgca gactgaatat ccgttttgtt
    30301 atgggttaac ttgcgcggtt cgccgccctg tggcgagata agccacagtt gcacaatgcc
    30361 gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc gcacccagtg
    30421 gcgcgggacg ttgactaacc ccggataagc ccgatgatgg gtaaaggtta aacgtcgctg
    30481 cacgacgcca cgcggtggcg cgggtagggt tgtttccgtt ccacttaacg gcgcatcacc
    30541 tgccgatttc cagctagctt catcttgcgg taactcaacg ataaatagct ccggcacttt
    30601 ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc
    30661 ttcttcataa gcacgattga tttcatcact gccaggctgc ggcgtgggcg tggttttact
    30721 caccagcaca caccagtggc taccgctgta ttcacgcgga tgttgctttt gtacgttgac
    30781 cgggccaaac ggtgcagcaa ccccgacgtt tcgcaaatcc agcgccggat cgagttgatg
    30841 cattacatgg tcgttatagg taaagctccc cctttcaccg ttcgggctaa agacatgcac
    30901 atggctgcca ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag
    30961 attgctcacc ttgccgcctt cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca
    31021 tgtttcatca ggattttcag ggccgtgaat aaagacatat ttctctgact tcggatgaac
    31081 ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga cctcgcctgt
    31141 atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc
    31201 aaataccagc cactggctgt cgggcgtcca ggtattggta ttggtgagca ggtgattacg
    31261 gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacggttc ccgccatcat
    31321 acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga atgtgagtga
    31381 aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcagggt cggcgttggc
    31441 acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat aaaaagcacc agtgcggtac
    31501 tgaaggtttc agcaaacctt gcagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg
    31561 ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg
    31621 atgaaatcgc tgatccccgc cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc
    31681 tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggctggga gcaacatatt
    31741 accgcccgct atctggtcgg tgccgatggc gcaaactcga tggtgcggcg acatctctac
    31801 ccggatcatc aaatccgtaa atatgtcgct atccagcagt ggttcgcaga gaaacatccg
    31861 gtgccgttct actcctgcat ctttgataat gcgataactg actgttactc atggagtatc
    31921 agcaaagatg gttattttat ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt
    31981 ttcacgacgc tgaaagagaa aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa
    32041 agtgagaaat gcacggtgtt gtttccctca cgctggcagg attttgtctg tggtaaggac
    32101 aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggatt
    32161 agctatgcgc tggatagcgc agagattctg cgtgcggtgt tcctgaaaca gccggagaag
    32221 agcaacgccg cttactggcg cgccacacgc aaactgcgtt taaaactctt cggcaagata
    32281 gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg
    32341 gcgcatattc cacagttgaa agattatcca acgcgcttca catcacccac cagcaggatg
    32401 taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg
    32461 taaccctgcg ccaggtaccc caccaccagc ggaacggtga tgccgcccag accaccggcg
    32521 aagttaaaca cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agagaccagc
    32581 gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca gcgccatcag gcacataatc
    32641 atcatcggat cgttggtgta gttagcgccc ataatgcagg tggagatcag caagccgcag
    32701 ataatcggcg ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgcg
    32761 acccagccag agagcaggac gccgacaaac gccgcgagga atggcaccgt ggtcataaag
    32821 cctgctttca gcgccgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag
    32881 aaaaaccaca gtgcagaagc caccgcaaat tgcccaagat aaacgccgat cagtttacga
    32941 tggaacacca gtttccagtc tttggctgtt aacggctgac gcgcctcttt cttcaccggc
    33001 gcatcaccat ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgata
    33061 cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg
    33121 ataccaccag tgacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc
    33181 agcggagtca aaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcggaa
    33241 gcgcgttcat gttccggaaa ccagctggtc accatccggt tattggtcgg gaaagcgggc
    33301 gcttcgaaaa taccggttat cgcacgcaga ccaattaatg acattaatcc cgtggcaaag
    33361 ccctggaata aagtcgccac tgaccaacca aatatcgcaa taaaataagt cacgcgagaa
    33421 cctacgcgat ctaaaaacca accgccgggg atctggcata gcgtataaag ccaggcgaag
    33481 gccgaaaata catagcccat ttccgctttc gtaatgccga actcttcctg aatatgggcg
    33541 gaagccacgg ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc
    33601 accagcgtca gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg
    33661 agctttctcc agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg
    33721 gttatatttt tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc
    33781 actctgctac gctgttatct tcatgacgcc agagcggatt acgccagtcc ggggcatttt
    33841 tactgaactc aataacttta gcttcgtcga tttccacacc taagcccggt ttcgttaacg
    33901 gtttaaagaa gccgccaacc atgctgaagt cttctttgtt tttcacaaag tcgagtaact
    33961 ccgcgccttt gttgtaatga atgcccatac tttgttactg aagtaccgcg ttataggaaa
    34021 caaagtcgat atgcaggcaa gccgccagtg caatcggtcc gaacggacag tgcggcgcaa
    34081 gggtcacatc ataggcttct gccattccag caattttgta gcattcggta ataccgcctg
    34141 catgggagag atccggttgc agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat
    34201 cgaaacgtga gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccaatt
    34261 tcgggtagta ttcggcttgt tccgccagca ccggctcttc aataaacagc gggcgatacg
    34321 gctccagttc tttaatcagc actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa
    34381 gaccaaactc aatctgattg ccaaaagctt cacgaatttg tgccacggtg ttgactgccg
    34441 catctaccgc gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg
    34501 tatcgaagcc gatttcgcgc agggttttaa tgccgtcgat aacatccgcc ggacgatcgc
    34561 cgccgaccca actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga
    34621 ctggcgcatt cagcactttg cctttgatat cccataacgc ctggtcgatc ccggcgatgg
    34681 cgctcatcag gattggaccg ccacgataaa atccggcgcg atacatcact tgccataagt
    34741 cgttgatgcg cgaaggatcc tgaccaatca aatagtcacc cagctcgtga actgccgctt
    34801 ccaccgtacg ggcgcggcct tcaatcacgg gctcgcccca accgaccacg ccttcatcgg
    34861 tttcaatttt caggaacatc cagcggggag gtaaacgata cgtggtaatt ttggtgattt
    34921 tcattgcact gcctctcgat acgccttaac aaatgctgct gcctgctgcg cggtgcgttc
    34981 tacggactgc ccggcgcgat aaagatcgct gcctaagccc gccccaacac aacctgcgtc
    35041 tatccactgc gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg
    35101 tggtaatacc acttttaacg ctttgatgta ttgcggacca aaagccgatg acggaaatat
    35161 tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc
    35221 ggggcaaacg gtcatgccgt agcccaccgc gcggcggatc acttcactat ggatattggg
    35281 cgtaacgatg agctgacagc ccatcctggc gagcgcatcg acctgttcag gtttcagtac
    35341 cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctctg
    35401 ctcccattgt ggggaattca gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc
    35461 gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag
    35521 tttagtttgc cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg
    35581 ccaccgccgc cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg
    35641 atgttccggc gacaagggtg atggcgtgtt gatgggtcac atagtcgcgc atactggcaa
    35701 cctctgcgcc aatcaacaaa ccagagagaa attcgctgac ctgttcgcgg agaagtgttc
    35761 ccagcacatg cgaggcgcga acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc
    35821 cgcgctcaag gccagccgcg aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc
    35881 caatcaatga gtgatttaac agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat
    35941 cgttgatttg ctggctatcg gcctgcaccc atttgcaatg cgtaccgggc ataacataaa
    36001 gagaggaagg agccagagct cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat
    36061 tgtggttatc gtcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag
    36121 acgttaattg ttcgccaata gacgaaaaac gggcaggaac agataaatac ggagcaactt
    36181 tccagccgac attgctgccg accattcctg ccatcaccac tggcgtattt tcttcacgcc
    36241 agtcggtcgt gacttctgct aacaccgcag ccggagattt tccgttcagg cgcgtgacgc
    36301 ctgcttctga ttgcctgctc tccaggcagt ggtcgccctg ataaagccag gcgcgcagat
    36361 tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg
    36421 tgtcgagctg gcaatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg
    36481 tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc
    36541 catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc taagttgctg
    36601 taacaccggg ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt
    36661 aaacgcttcg cggtcctgat tgttggcaat catctcgttc agcgccgatt caatctgcgc
    36721 cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt
    36781 tcgcacttcg ctcatggcac tgataagccg tgggtcgtag tcgttttcca gcacccattg
    36841 cagtacgtca gtgtcgaggt aattccactg gttacgcggt gccacaaacg ccccgcgata
    36901 acgtttcatt tcaatcagcc gcttcgccat cagcgaacgg aacacctcac ggatgatgtt
    36961 gcgcgaggtt gcaaactctt cacagagctc cgcctcagcc ggaagtggcg agccaggtac
    37021 gtatttgccg tgaacgatct gtttacccag cgtaatgaca atgcgatcgg ttttattgag
    37081 agtcatggag agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata
    37141 acgcggcaac tttgtagtac cagcgtgatg acgttcgcgt ttgccgtgcg tgtaatgtag
    37201 tacaaactta tattgttgtg ctacaattta gatcaaaaaa agaacaatgt ataaaaaaat
    37261 gacatgcgtc gggcagaaat ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag
    37321 agtatgcttt caacggctta gctactcgtt taaaagatta atcatgaagt tgaattttaa
    37381 gggatttttt aaggctgccg gtttattccc actggcgctg atgctttcag gctgtatctc
    37441 gtatgctctg gtttcccata ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga
    37501 caccatcact gggctatcgc aggcaaaaga tagtaatgga acaaaaggct atgtttttgt
    37561 aggggaatcg ctggattacc ttatcactga tggtgccgat gacatcgtta agatgctcaa
    37621 tgatccagca cttaaccggc acaatattca ggttgccgat gacgcaagat ttgttttaaa
    37681 tgcggggaaa aagaaattta ccggcacaat atcgctttac tactactgga ataacgaaga
    37741 agaaaaggca ctggcaacgc attatggttt tgcctgtggt gttcaacact gtaccaggtc
    37801 actggaaaac ctaaaaggca caatccatga gaaaaataaa aacatggatt actcaaaggt
    37861 gatggcgttc taccatccat ttaaagtgcg attttatgaa tactattcac ccagaggcat
    37921 tccggatggt gtttccgcag cattactgcc agtgactgtt acgctggaca tcattactgc
    37981 accgctgcaa tttctggttg tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg
    38041 tttatgctgt ttgcccgccc acagattaat tcagcacata cttctcaata gcaaacgcca
    38101 cgccatcttc aaggttggat tttgtgacaa agttcgccat ttctttcacc gaaggaatag
    38161 cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg ttttcctgat
    38221 cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt gatttcacac
    38281 ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca cttttcagca
    38341 cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg
    38401 gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc tccgcttcgc
    38461 agaacaccag cggaattgtg gcaacgaagg attcatgcac cgtgtagtag ctgatatcgc
    38521 ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag
    38581 agagtttttc caggaaacga tagtcgtcat agctgagagc ggtttgcgcc acggtgctac
    38641 catcagcggc cttctgtacc agcgcgccgt tataagtaat gcagtagtcg cccggctgtt
    38701 ccatatgcag ctctttcagg tagttgtgca cacctgcata cggacgaccc gtcgttagca
    38761 cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg
    38821 tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg atagccatga
    38881 gttcccaagg tagattagtt cctgacccat gctaacgcga ttccgctcaa aaatcagtac
    38941 aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa
    39001 gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtgctga
    39061 cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact
    39121 ggaagccgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc
    39181 gagcgccgat tccagttgct cgccgctaac tgattgattt ctctgttgat tactcaacca
    39241 agttgagaga atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc
    39301 accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa
    39361 ggcaccgaca acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga
    39421 cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa aagggcggag
    39481 atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc
    39541 ctacaaaatc atgcaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg
    39601 catcaggcac gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    39661 ttctcttcaa taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac
    39721 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    39781 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    39841 gagaggccac gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    39901 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    39961 tcacccagcg tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga
    40021 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    40081 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    40141 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc
    40201 aacgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    40261 ttgatcattt tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc
    40321 aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    40381 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    40441 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga
    40501 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    40561 atgatgatgc tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag
    40621 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    40681 ttctcgacgt taaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc
    40741 ccctgcttcg cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc
    40801 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct
    40861 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    40921 attttgccaa ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt
    40981 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    41041 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    41101 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    41161 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    41221 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    41281 gccacttcaa cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    41341 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    41401 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    41461 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    41521 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    41581 gccagcggag cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    41641 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    41701 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    41761 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    41821 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    41881 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    41941 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    42001 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    42061 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    42121 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    42181 cgcttttaag cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc
    42241 atctatcagg tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag
    42301 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    42361 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    42421 gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    42481 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcatccat
    42541 atcggccgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    42601 gatcagttct ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    42661 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc taggttcgtt
    42721 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    42781 aaacccttct ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg
    42841 cccgtccgta ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    42901 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg
    42961 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    43021 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    43081 agataaagcg agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag
    43141 ggacattaca gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca
    43201 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    43261 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    43321 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc
    43381 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg
    43441 cgagcaaacg cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc
    43501 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag
    43561 tcgccaacgt gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg
    43621 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt
    43681 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg
    43741 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc
    43801 tgatgcgcca tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg
    43861 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    43921 gacagcgaaa aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg
    43981 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    44041 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    44101 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    44161 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    44221 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    44281 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg
    44341 aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt
    44401 agtcagctct ttcgctagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg
    44461 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    44521 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    44581 gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc
    44641 cccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc
    44701 cggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag
    44761 ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc
    44821 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    44881 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    44941 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    45001 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    45061 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    45121 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    45181 cgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    45241 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    45301 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    45361 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    45421 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    45481 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacaggga
    45541 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    45601 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    45661 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    45721 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    45781 caagatcctg caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    45841 tgtttctcac agattttccc tatttatcca caggactttc cagaactcgc taagtgtaaa
    45901 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    45961 acaaaaattg gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccatc
    46021 agcccgtata atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca
    46081 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt
    46141 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    46201 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    46261 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    46321 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    46381 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    46441 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    46501 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    46561 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    46621 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    46681 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    46741 atggcgccgc cactgtctcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    46801 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    46861 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    46921 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    46981 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    47041 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    47101 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    47161 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    47221 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    47281 cccagccgtt ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    47341 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    47401 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    47461 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    47521 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg
    47581 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    47641 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca
    47701 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    47761 cgatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    47821 tctataccgc taatctgggt aacggcatcg tcgctatcgg ctataaatct cagccggtac
    47881 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    47941 aggacaaaat ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt
    48001 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    48061 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    48121 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    48181 gtgagcgtct gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag
    48241 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    48301 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    48361 ggatccacga cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa
    48421 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    48481 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    48541 tactgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    48601 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    48661 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    48721 gcgcatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    48781 aggccacacc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    48841 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    48901 cgtttaaaga cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    48961 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    49021 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    49081 agttttccga acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg
    49141 ccgatcttat cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag
    49201 gcgcattctc cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg
    49261 tcgaagcggc aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg
    49321 aagcccagct taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcacgtcagg
    49381 gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat
    49441 cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa
    49501 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    49561 acactgccgg gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt
    49621 ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    49681 ccgttgatcc ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    49741 tcaccgtggt acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga
    49801 acggtcacgc gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgc
//
LOCUS       DAJTYI010000013        47834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYI010000013 DAJTYI010000000
VERSION     DAJTYI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(18..93)
                     /locus_tag="OO564_000760"
     tRNA            complement(18..93)
                     /locus_tag="OO564_000760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Val,seq:tac)
     gene            complement(145..220)
                     /locus_tag="OO564_000761"
     tRNA            complement(145..220)
                     /locus_tag="OO564_000761"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Lys,seq:ttt)
     gene            complement(223..298)
                     /locus_tag="OO564_000762"
     tRNA            complement(223..298)
                     /locus_tag="OO564_000762"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Val,seq:tac)
     gene            complement(435..510)
                     /locus_tag="OO564_000763"
     tRNA            complement(435..510)
                     /locus_tag="OO564_000763"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Lys,seq:ttt)
     gene            complement(675..1466)
                     /gene="cpoB"
                     /locus_tag="OO564_000764"
     CDS             complement(675..1466)
                     /gene="cpoB"
                     /locus_tag="OO564_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6651998.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1476..1997)
                     /gene="pal"
                     /locus_tag="OO564_000765"
     CDS             complement(1476..1997)
                     /gene="pal"
                     /locus_tag="OO564_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6651999.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2032..3324)
                     /gene="tolB"
                     /locus_tag="OO564_000766"
     CDS             complement(2032..3324)
                     /gene="tolB"
                     /locus_tag="OO564_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6652000.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3457..4713)
                     /gene="tolA"
                     /locus_tag="OO564_000767"
     CDS             complement(3457..4713)
                     /gene="tolA"
                     /locus_tag="OO564_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6652001.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4778..5206)
                     /gene="tolR"
                     /locus_tag="OO564_000768"
     CDS             complement(4778..5206)
                     /gene="tolR"
                     /locus_tag="OO564_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6652002.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5210..5902)
                     /gene="tolQ"
                     /locus_tag="OO564_000769"
     CDS             complement(5210..5902)
                     /gene="tolQ"
                     /locus_tag="OO564_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6652003.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5899..6303)
                     /gene="ybgC"
                     /locus_tag="OO564_000770"
     CDS             complement(5899..6303)
                     /gene="ybgC"
                     /locus_tag="OO564_000770"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6652004.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6453..6746)
                     /gene="ybgE"
                     /locus_tag="OO564_000771"
     CDS             complement(6453..6746)
                     /gene="ybgE"
                     /locus_tag="OO564_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6652005.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6746..6859)
                     /gene="cydX"
                     /locus_tag="OO564_000772"
     CDS             complement(6746..6859)
                     /gene="cydX"
                     /locus_tag="OO564_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6652006.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6874..8013)
                     /gene="cydB"
                     /locus_tag="OO564_000773"
     CDS             complement(6874..8013)
                     /gene="cydB"
                     /locus_tag="OO564_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6652007.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8029..9597)
                     /gene="cydA"
                     /locus_tag="OO564_000774"
     CDS             complement(8029..9597)
                     /gene="cydA"
                     /locus_tag="OO564_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6652008.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10446..13079)
                     /gene="mngB"
                     /locus_tag="OO564_000775"
     CDS             complement(10446..13079)
                     /gene="mngB"
                     /locus_tag="OO564_000775"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6652009.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(13097..15013)
                     /gene="mngA"
                     /locus_tag="OO564_000776"
     CDS             complement(13097..15013)
                     /gene="mngA"
                     /locus_tag="OO564_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6652010.1"
                     /translation="MNLTTLTHRDALCLNASFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            15183..15905
                     /gene="mngR"
                     /locus_tag="OO564_000777"
     CDS             15183..15905
                     /gene="mngR"
                     /locus_tag="OO564_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6652011.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(16009..16878)
                     /gene="sucD"
                     /locus_tag="OO564_000778"
     CDS             complement(16009..16878)
                     /gene="sucD"
                     /locus_tag="OO564_000778"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6652012.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16878..18044)
                     /gene="sucC"
                     /locus_tag="OO564_000779"
     CDS             complement(16878..18044)
                     /gene="sucC"
                     /locus_tag="OO564_000779"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6652013.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18138..19355)
                     /gene="odhB"
                     /locus_tag="OO564_000780"
     CDS             complement(18138..19355)
                     /gene="odhB"
                     /locus_tag="OO564_000780"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6652014.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19370..22171)
                     /gene="sucA"
                     /locus_tag="OO564_000781"
     CDS             complement(19370..22171)
                     /gene="sucA"
                     /locus_tag="OO564_000781"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6652015.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22472..23188)
                     /gene="sdhB"
                     /locus_tag="OO564_000782"
     CDS             complement(22472..23188)
                     /gene="sdhB"
                     /locus_tag="OO564_000782"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6652016.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23204..24970)
                     /gene="sdhA"
                     /locus_tag="OO564_000783"
     CDS             complement(23204..24970)
                     /gene="sdhA"
                     /locus_tag="OO564_000783"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6652017.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24970..25317)
                     /gene="sdhD"
                     /locus_tag="OO564_000784"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24970..25317)
                     /gene="sdhD"
                     /locus_tag="OO564_000784"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6652018.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25311..25715)
                     /gene="sdhC"
                     /locus_tag="OO564_000785"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25311..25715)
                     /gene="sdhC"
                     /locus_tag="OO564_000785"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6652019.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26096..26203
                     /locus_tag="OO564_000786"
     CDS             26096..26203
                     /locus_tag="OO564_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652020.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26409..27692
                     /gene="gltA"
                     /locus_tag="OO564_000787"
     CDS             26409..27692
                     /gene="gltA"
                     /locus_tag="OO564_000787"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6652021.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28083..28649
                     /locus_tag="OO564_000788"
     CDS             28083..28649
                     /locus_tag="OO564_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6652022.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28700..31159
                     /locus_tag="OO564_000789"
     CDS             28700..31159
                     /locus_tag="OO564_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6652023.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            31174..31905
                     /locus_tag="OO564_000790"
     CDS             31174..31905
                     /locus_tag="OO564_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6652024.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31902..32964
                     /locus_tag="OO564_000791"
                     /pseudo
     CDS             31902..32964
                     /locus_tag="OO564_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            33116..34162
                     /locus_tag="OO564_000792"
     CDS             33116..34162
                     /locus_tag="OO564_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6652025.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSTGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34159..34950)
                     /gene="nei"
                     /locus_tag="OO564_000793"
     CDS             complement(34159..34950)
                     /gene="nei"
                     /locus_tag="OO564_000793"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6652026.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34986..35720)
                     /gene="pxpA"
                     /locus_tag="OO564_000794"
     CDS             complement(34986..35720)
                     /gene="pxpA"
                     /locus_tag="OO564_000794"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6652027.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35710..36642)
                     /gene="pxpC"
                     /locus_tag="OO564_000795"
     CDS             complement(35710..36642)
                     /gene="pxpC"
                     /locus_tag="OO564_000795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6652028.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36636..37292)
                     /gene="pxpB"
                     /locus_tag="OO564_000796"
     CDS             complement(36636..37292)
                     /gene="pxpB"
                     /locus_tag="OO564_000796"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6652029.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37315..38058)
                     /gene="ybgI"
                     /locus_tag="OO564_000797"
     CDS             complement(37315..38058)
                     /gene="ybgI"
                     /locus_tag="OO564_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6652030.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38329..39810
                     /gene="dtpD"
                     /locus_tag="OO564_000798"
     CDS             38329..39810
                     /gene="dtpD"
                     /locus_tag="OO564_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6652031.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39960..41378)
                     /gene="phrB"
                     /locus_tag="OO564_000799"
     CDS             complement(39960..41378)
                     /gene="phrB"
                     /locus_tag="OO564_000799"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6652032.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(41375..41884)
                     /locus_tag="OO564_000800"
     CDS             complement(41375..41884)
                     /locus_tag="OO564_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6652033.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            42013..42330
                     /locus_tag="OO564_000801"
     CDS             42013..42330
                     /locus_tag="OO564_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6652034.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(42385..42765)
                     /locus_tag="OO564_000802"
     CDS             complement(42385..42765)
                     /locus_tag="OO564_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652035.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(42768..47432)
                     /locus_tag="OO564_000803"
     CDS             complement(42768..47432)
                     /locus_tag="OO564_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6652036.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            complement(47451..47654)
                     /locus_tag="OO564_000804"
                     /pseudo
     CDS             complement(47451..47654)
                     /locus_tag="OO564_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11332 a        13148 c        11633 g        11721 t
ORIGIN      
        1 tgagctaacg acccgagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
       61 agggaggtgc tctcccagct gagctaatca cccgatactg cgctggatac tacaaactac
      121 ttccaccgaa cccaccgtaa ttagtggtgg gtcgtgcagg attcgaacct gcgaccaatt
      181 gattaaaagt caactgctct accaactgag ctaacgaccc ggtggtgggt gatgacggga
      241 tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccga
      301 cactacgctg gatactacaa ctaactatgt tagtggtggg tcgtgcagga tgactcgact
      361 tcgtctcgcc cttcggcgcc gttgctgatg caacgttatc cttcacattt tactaccgct
      421 tccaccttag cgattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
      481 caactgctct accaactgag ctaacgaccc actttttcgt tgctttcggt ttgtttgata
      541 tcccgtggca acggcggcat atattactga tttcagactt gagcgcaaca aaaatttcga
      601 tgcagatcac tcaactgctt atgattcgca cgacacgacc agaaataatg cgacttctgg
      661 tcgtgtttta tgcattacat cgcgttcaga cgtttttgtg cctgtttagc gccatcagta
      721 ccagggtatt tactgataac ctgctggtat acggctttcg cttttgcggt gtcacctttg
      781 tcctgcatga tgacgccgac tttaaacatc gcatcagcag cctttggtga cttcggatag
      841 tttttcacta ccgaagcaaa atagtacgcc gcatcatctt ttttaccctt gttgtagttt
      901 aactgaccca gccaataatt ggcgtttggc aggtaagttg aatcagggta atttttgatg
      961 aaattctgaa atgccaccat tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata
     1021 gctgcattgt aatccgtgtt tgcatcgccg cttttcaccg gcgcgccagc attcgcagta
     1081 ccagcatcag ctgtcggcgt cgttgatgcc gccgcaccgc tttgatcgct gctggttgat
     1141 tgcgccgctg caccaccgct gctgaggctg tcgatctgca acaggatctg cttttgccgc
     1201 tccacgacct gattcagttg atactgattt tcctgaatct gaccacgcag ggaatcaata
     1261 tcggattgat tatcagaaag ttgttgctgg agttgggtta aaagctggct gtgagcatta
     1321 gaaatacgct caagttgagt gacgcggtct tcgaccgagc ctgagccgac actactgatt
     1381 ggtgcctgag caaaagcggc ccagggggcc gctataccaa ccagtaacga cagactcaat
     1441 agttgatgtc tgaagttact gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg
     1501 tttttggagt atgctgcttc gtcatgaccc agtactgcag gtttttcttt accgtaagaa
     1561 acgatggaga tctggtctgc agaaacgcct ttaccctgca ggtacatctt aacggcgttc
     1621 gcacgacgtt cacccaggga gatgttgtat tccggagtac cacgttcgtc cgcgtgacct
     1681 tctacggtga ctttgtaaga cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt
     1741 tgagcgaagt cagaacggat atcgtacttg tccagatcga agtaaacgat gttgttctgc
     1801 tgcagctgtt gcatttgcag acgagcctgc tcttcggaag acatgttgcc gttgccgccg
     1861 ttcgcatcca taccagtgcc agcacccagc atgccttcgc tgccgtcatt gctggcgttc
     1921 ttgttggaag aacatgccgc aattgccata acaggcagag caatcatcag ccctttcagc
     1981 actttgttca gttgcatttc aatgattcct ttactattca attaattatt atcacagata
     2041 cggcgaccag gcagggaatt tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg
     2101 cccatctgta gaaaccaaat tcagcacgga tcccatcccc tgagaagagc tgtagattac
     2161 catagtgccg ttaggtgcca gacttggcgt ttcatccagg aacgtggacg acagaacttg
     2221 tacgcctccc gttgccagat cttgtttggc aatgtgctgc tgcccaccat tggagctgac
     2281 cattaccata aatttaccgt cgctgctgac atccgcatcc tggttctgcg aaccttccca
     2341 ggtaatacgt tgtggcgcac cgccgttgat attcacttta taaacctgcg gacgaccggc
     2401 ctggtcagaa gtaaatgcca ggttctggct atccgggaac caggtcggtt cggtattgtt
     2461 actgcgacca tcagtcacct ggcggatctg accagaagcc aaatccatta cgtacaggtt
     2521 cagactaccg gttttcgaca aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg
     2581 tgcaccgttg tgacgcggga atgaagccac ctgacgtaca gcgccatttg ccagcgtctg
     2641 aataaccagc gcggaacgac cgctttcgaa ggtcacataa gccagtttag aaccgtctgg
     2701 tgaccacgcc ggagacatca gcggctgcgg tgaacggtga acgacaaact ggttgtaacc
     2761 gtcatagtca gatacgcgca gttcatacgg gaactgaccg ccgttggtct gaacaacgta
     2821 ggcaatacgg gtacggaacg cacctttaat gccggtcagt ttttcaaaca cttcatcact
     2881 ggcggtatga ccagcataac gcagccactg cttgttcact ttgtacgagt tctgagcaag
     2941 tacagtaccc ggtgcgccgc cagtgtcaac aagttgataa gcaacattgt aagaaccatc
     3001 cggattcgga gtgacctgac cgacaactac agcgtcaatg cccagtgcgg accatgcagc
     3061 tggttgtact tcctgcgcac tacccggctg ctgtggcaga cgagcgcgat ctaacggatt
     3121 aaatttaccg ctgttacgca agtcagcagc aacgatgccg ccaatatctt caggtgccgc
     3181 accaggcccc gcccactgga aaggaacaac accaatagga cgaccggaat ctacaccgct
     3241 gtcgatcaca atgcggactt cagcatgcag aactgatgcc cacagtatga gaaaaccaaa
     3301 tgctactcgt aatgcctgct tcatcatatc tcccttatct ggaccgatgg cccacgataa
     3361 tttagcagaa tgttaacaaa ctaaaataca caaaactacc agaaccccgt ggcaaccggt
     3421 gcctgatgtt gaccgtccga acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc
     3481 gtttttgaac acttcatata ctgcctggct tggtggtttc gggatcttcg caagtttagc
     3541 tgctgccaac gcagcctgac aaagtgcggg atcgccacct tcaggtttga tatccagtaa
     3601 cataccatcg ggtgccagtt ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc
     3661 gtcatagaac ttactttcga tagcagattt aatctgcccg gcatagttat tgatatcggc
     3721 ccctgatgcg ccattgtttt tagtattacc actcccggca ggcgaagcat tgttcccttt
     3781 cgcccctccc cccgttttcg gtgcattctt accagagctt agctcaccga aaatatcatc
     3841 tgcctctgcg gcagcttttg ctgcagcggc tttttcagct gctgcttttg ccgctgccgc
     3901 ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg ccttatcagc
     3961 agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg ctttcttatc
     4021 agctgcagcc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
     4081 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
     4141 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
     4201 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
     4261 agcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
     4321 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
     4381 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
     4441 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
     4501 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
     4561 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
     4621 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
     4681 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
     4741 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
     4801 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
     4861 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
     4921 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
     4981 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
     5041 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
     5101 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacggt acaatgttga
     5161 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
     5221 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
     5281 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
     5341 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
     5401 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
     5461 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
     5521 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
     5581 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
     5641 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
     5701 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
     5761 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
     5821 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
     5881 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
     5941 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
     6001 attctcggcg ttgacaatat gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
     6061 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcataatatt ccaccgtcat
     6121 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
     6181 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
     6241 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
     6301 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
     6361 cgcgattgag gtttgggaat gagggcaagt taagggagcg taaaaattta tgggcctaga
     6421 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
     6481 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
     6541 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
     6601 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
     6661 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
     6721 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
     6781 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
     6841 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
     6901 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
     6961 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
     7021 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
     7081 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
     7141 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcggca gaaccacacc
     7201 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
     7261 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
     7321 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
     7381 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccccggt
     7441 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
     7501 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
     7561 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
     7621 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
     7681 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
     7741 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
     7801 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
     7861 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
     7921 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
     7981 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
     8041 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
     8101 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
     8161 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
     8221 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
     8281 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
     8341 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
     8401 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
     8461 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
     8521 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
     8581 cgcctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
     8641 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
     8701 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
     8761 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
     8821 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
     8881 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
     8941 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
     9001 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
     9061 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
     9121 gaaatcggac gcgattgggt tttgcatcca gccgttcgca atcagaatcc acagtgcgga
     9181 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
     9241 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
     9301 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
     9361 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
     9421 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
     9481 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
     9541 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
     9601 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
     9661 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
     9721 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
     9781 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
     9841 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
     9901 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
     9961 ttaatcatgt ttcaacaagg taactttagt gacatttatg tttaaaatgt gtgaattata
    10021 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    10081 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    10141 aataaaggca taaatctcct ccccgccgat attaacattt aattaactta taattatatt
    10201 ataattaact cacccggggt tattattttg ttaaaaataa agccacatat acgtagcttg
    10261 agagtgaaga taaacgcaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    10321 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    10381 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    10441 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    10501 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    10561 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    10621 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    10681 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    10741 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    10801 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    10861 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    10921 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    10981 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    11041 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    11101 ggcgcttctt tccagccttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    11161 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    11221 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    11281 ctgttgttat gactaagagt gacaaccaac tcgacaccta ctctgccagt ggattgtctt
    11341 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    11401 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    11461 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    11521 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    11581 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    11641 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    11701 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    11761 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    11821 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    11881 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    11941 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    12001 gccagttcga agcgggcgac gatttcgcga tgaactttgt cactacagca gcagccgatg
    12061 ctgtcgtggg catgattttt taagatctct ttccatattt tctccagcaa gccgtggtgg
    12121 tattcaaaac ccaacgtcca ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    12181 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    12241 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    12301 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    12361 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    12421 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    12481 tcgaggcgtt tgcggaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    12541 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    12601 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    12661 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    12721 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    12781 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    12841 ccggcttcta ccagtttctt cacgcggtct ttgttttccg gtttcaccgc gaaataatct
    12901 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    12961 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    13021 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    13081 gtcgtttcct ttttgtttat tgcattacgc catcagtcag atagttgcca tgcttaaccg
    13141 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    13201 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    13261 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    13321 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    13381 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    13441 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    13501 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    13561 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    13621 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    13681 taagcgagtt attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg
    13741 ctcccaatgt accgagcacc aggtagagat aaaaagtcag gaagccattg aatttactgc
    13801 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    13861 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    13921 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    13981 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    14041 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    14101 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    14161 ccgttttgac gcttttcacc ggttgcgtat cttgctgtac ggtacgcgtc tcatcgccac
    14221 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    14281 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    14341 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    14401 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    14461 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    14521 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    14581 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    14641 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg gggggaatcg
    14701 ccagcaacac cactaaatca atcgcttccg ggccatccac gctttcccac tgaagcggct
    14761 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    14821 cagccaaccc ttcacctaag gcggtcgggc caaggctttc acggcgatac acttctttca
    14881 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    14941 tggcctcttc acggctggta aagctcgcat tcagacacaa cgcatcgcgg tgggtcagag
    15001 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgat
    15061 aaaacaatac cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tgtatttgta
    15121 ttatttacag gatgaattta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    15181 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    15241 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    15301 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    15361 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    15421 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    15481 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    15541 aggatcgtgt gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    15601 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    15661 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    15721 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    15781 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    15841 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    15901 gataagcaca aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
    15961 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
    16021 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
    16081 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
    16141 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt
    16201 aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
    16261 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt
    16321 agagccaggg atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc
    16381 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
    16441 acccggtttg tgaatgtgac ccggctggat accgattttg cattcacccg gggtgataac
    16501 gcctgggcag ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt
    16561 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
    16621 gatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga
    16681 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
    16741 agtgccgcct ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg
    16801 ttctgagtgg aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt
    16861 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
    16921 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
    16981 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc
    17041 gcgccgatga taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
    17101 agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
    17161 ttggttgcgc cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact
    17221 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
    17281 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
    17341 tggtcacgca tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt
    17401 ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc
    17461 agcgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc
    17521 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
    17581 gggccagtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact
    17641 ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg
    17701 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc
    17761 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag
    17821 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
    17881 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
    17941 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
    18001 tagcgggcaa acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
    18061 tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc
    18121 aggtgaagct taaactacta cacgtccagc agcagacgcg tcggctcttc cagcaactct
    18181 ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac
    18241 agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct
    18301 ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc
    18361 agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc
    18421 gtcagcttgc catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc
    18481 atgccgaggg tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc
    18541 gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc
    18601 acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc
    18661 agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc
    18721 ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc
    18781 agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca
    18841 gccagagccg gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct
    18901 ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta
    18961 atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca
    19021 tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct
    19081 tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc
    19141 tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct
    19201 gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt
    19261 acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca
    19321 ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt
    19381 tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg
    19441 ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt
    19501 cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc
    19561 agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga
    19621 acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct
    19681 gttccagcag gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg
    19741 gatcaagttc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga
    19801 ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac
    19861 gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg
    19921 gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg
    19981 aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca
    20041 tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt
    20101 tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat
    20161 aaccatattc aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg
    20221 cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt
    20281 ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa
    20341 ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga
    20401 acagtttctc accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact
    20461 gcatttcaac tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca
    20521 tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga
    20581 aagagtgcat gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat
    20641 cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca
    20701 gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg
    20761 gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt
    20821 cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg
    20881 caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga
    20941 tatcagtaca gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct
    21001 ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt
    21061 tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg
    21121 gcagcacttt gttgctgctc ggctcatcaa gtctgtccag acgggcacga acagagccga
    21181 taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca
    21241 ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc
    21301 cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca
    21361 gcacgttcac cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg
    21421 tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc
    21481 cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt
    21541 cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac
    21601 cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact
    21661 cggcaccaat cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca
    21721 tggtttcttt gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg
    21781 tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg
    21841 gatctagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt
    21901 taacctgctt cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt
    21961 tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg
    22021 taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta
    22081 agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt
    22141 ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct
    22201 tacttcgccg tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct
    22261 cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt
    22321 ttttaaagat ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg
    22381 ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg
    22441 caacgcttgc gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact
    22501 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
    22561 tgatgctgtg acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag
    22621 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
    22681 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
    22741 tacattcata cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
    22801 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
    22861 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
    22921 caatcttctt gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct
    22981 tgccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
    23041 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac
    23101 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
    23161 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
    23221 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
    23281 ccgactcagg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
    23341 cgaagcggct atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat
    23401 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
    23461 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa
    23521 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
    23581 gcagcgcttt acggatcgcc accggatctt caccgttacg attattgttc caccggttca
    23641 ggcgatccag agacgcttca acatcagact cgctggcatc gcgcagtgcg ccctgctcgg
    23701 cgatagactc ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg
    23761 agttgccgcc cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa
    23821 acagtcccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa
    23881 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
    23941 tcaccggatc gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt
    24001 cgagaacttc tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat
    24061 cacagccgcg accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg
    24121 ccaggtcttt ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca
    24181 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg
    24241 tcgggtggaa ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc
    24301 cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgccc
    24361 cgccagtcgc cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc
    24421 acagtgcggt acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc
    24481 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    24541 cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt
    24601 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca
    24661 ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    24721 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    24781 tatcttcatg ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg
    24841 aacgggtcgg gaagacttta gagagcagcg cacaggtctg gccgctctgg gaaatttgca
    24901 gcgccgcgcg catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg
    24961 gcaatttcat cacacccccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    25021 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    25081 cacctgccac atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa
    25141 cactttggtg aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact
    25201 ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg
    25261 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    25321 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    25381 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    25441 acgacgacgt gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    25501 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    25561 agccacagca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    25621 gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    25681 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac
    25741 tacgggcaca gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc
    25801 tgcttgcggg tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca
    25861 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    25921 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    25981 gttttatcct gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc
    26041 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    26101 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    26161 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    26221 tacttgattc gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc
    26281 tgtacccagg ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    26341 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    26401 gaccttaaat ggctgataca aaagcaaaac tcaccctcaa cggggacaca gctgttgaac
    26461 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    26521 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    26581 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    26641 cgaccgattc taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    26701 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    26761 ttacccgtct gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta
    26821 ttaccggcgc gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    26881 gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca
    26941 agtattccat tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact
    27001 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    27061 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca
    27121 ccgtgcgtac cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg
    27181 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    27241 aaattagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt
    27301 ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg
    27361 taatgcgtga aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg
    27421 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac
    27481 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    27541 ccatgttcac cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg
    27601 aaatgcacag ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    27661 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt
    27721 aacccgccgt tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa
    27781 taaaatccac tatcggacat tcatatattc taaaaaaaaa tatatcggta taaattataa
    27841 acaaatcgat gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat
    27901 atagcgcacc cctataattt ccaatacttt attcatcaaa catttaattc ataccatcaa
    27961 ttaattactt ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg
    28021 acaatcaaat aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag
    28081 ttatgtttaa aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac
    28141 ctgtttatgc ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag
    28201 caccgtgtga aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca
    28261 ccagccacat taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca
    28321 actgtgatct ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca
    28381 cctttgatag ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg
    28441 gtgaagccac ctgcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag
    28501 gcaccgctac tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct
    28561 ttgcctggat ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga
    28621 atgcaaccta cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt
    28681 cataaaatat ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg
    28741 cctggtcgtg gcgatgcctt gcgcattggc gctcgaattc aatctgaatg ttctcgataa
    28801 atcaatgcgc gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg
    28861 tgagtatttt gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa
    28921 ctggcacaaa aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt
    28981 tggcttaaaa cctgaagtcc gtcagtcatt accattgata aatcagtgcg tcgattttag
    29041 ctcccgacct gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat
    29101 tccgcaagcc tggctggcgt ggtactcaga aaactggacc ccaccctcca catggaaaga
    29161 aggtgtcgcc ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga
    29221 tggcagcagc agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg
    29281 gcgcttacgc agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc
    29341 aggcgaaata tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac
    29401 cctcggcgaa acggatttta gttccaatat tttcgacggt ttttcttata ccggcgcggc
    29461 actggcaagt gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag
    29521 cggtattgca cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca
    29581 gaaaaaagtc ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac
    29641 actggatgtc aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc
    29701 atcgacgccc ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc
    29761 acggccctcc atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg
    29821 ggggatgctg tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca
    29881 ttctgccgca atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt
    29941 cacgtgggcc agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt
    30001 taattacagc aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt
    30061 ctccgatcgt cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga
    30121 tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa
    30181 cctcaatctt tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac
    30241 cgccaacatc acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc
    30301 tacgtcattc aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc
    30361 gatttcgctc cccttcggta acggtggtca ggttggctat gacatgcaaa acagtagcca
    30421 cagcaccaca caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat
    30481 gtctgccgga ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca
    30541 gcacctgagt tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag
    30601 ttccgtcagc agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca
    30661 tcgccgcagc tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat
    30721 tccggttcag ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat
    30781 ttccagttac cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac
    30841 agttgcagaa aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact
    30901 ggcttcccgt tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc
    30961 tcccctcgga gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga
    31021 ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg
    31081 tgatagccag cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct
    31141 gattttacct tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt
    31201 ttgttgttgg ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt
    31261 gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc
    31321 ccctacctcg cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg
    31381 gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa caacgcaggt acggatagta
    31441 aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg
    31501 cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt
    31561 cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag
    31621 gtcgaactgc agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc
    31681 gcgtattatc tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc
    31741 aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc
    31801 agccagtttt atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac
    31861 tgcagcggac aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag
    31921 tgttgctcgt catctgtctg ttatttctgc cgttgaagtc tgcgcttgcc ctgaactgct
    31981 actttggttc atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac
    32041 caggcaatgc caaactcggc gataagatct gggaatctga cgatattaaa attcccgtct
    32101 attgtgacaa caataccaac ggcaactttg aaagcgaaca tgtctacgca tgggtaaacc
    32161 cctacccggg ggtgcaggat cgttattatc aactcggagt aacttacaat ggcgttgatt
    32221 atgacgccaa ccagggaaaa agccgcatcg acactaacca gtgtatcgac agtaagaaca
    32281 ttgacattta tacccttgag cagatcatcg cgatgggatg gcagaacaaa atttgctcgg
    32341 gtgatcccgc taatattcac atgtcgcgta cctttctcgc tcgcatgcgg ttatacgtca
    32401 aaatacgaga aatgccgccg catgattatc aaagtacgct tagcgactat atcgtcgtgc
    32461 aatttgacgg tgccggtagc gttaacgaag accctactgc ccaaaacctg aaatatcata
    32521 ttactggtct ggaaaacatt cgcgtgctgg attgcagcgt caatttttcc atttcaccgg
    32581 aaacacaggt gattgatttt ggtaaattta atttgctgga tattcgtcgc cacacaatgt
    32641 cgaaaacatt cagtattaag acgacaaaat cacaaaatga ccaatgcacc gacggattta
    32701 aagtcagttc ctccttttat accgaagaaa cgttggttga agaagataaa gcattgttga
    32761 ttggtaatgg tctgaagcta cgtttgctgg atgaaaacgc ctcgccttat accttcaata
    32821 aatatgctga atacgccgat ttcaccagcg acatgttggt ctacgaaaaa acctatacag
    32881 ctgaactttc gtccattgca ggtaccccca tcgaggctgg ggcccttcga tacagtggtg
    32941 ctttttaaaa ttaactataa ctgatttgcc atgatgccct ttgcagccgg aaagggcatc
    33001 tctttcagaa aattgctgat tttccccttt tccttgattg ctgttatttt ttcctagccc
    33061 ggctttcttt atgctgtatc gatacaccaa agagaaaata atgagggagc gaaggatgcc
    33121 agttttgcag tggggaatgt tatgtgtgct atcacttctc ctttctattg gtttcctcgc
    33181 ggtacatctc ccggcggcgc tattacttgg gccgatgatc gccgggatca tcttcagtat
    33241 gcgcggaatt actctgcaac ttccccgctc cgcttttctc gccgcgcagg ccattcttgg
    33301 ctgcatgatt gcgcaaaacc tcactggttc gattctcacc acgctggccg ccaactggcc
    33361 gatcgtgctt gctattttac tggtgacgct gctttccagc gccatcgtgg gttggttatt
    33421 ggtgcgctat agctcactgc ctggaaatac cggtgcctgg ggttcttcta caggcggcgc
    33481 agcggcaatg gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat
    33541 gcaatatctg cgagtgctgt tcgtcgcggg tgccgctgtt ctggtcaccc gcatgatgct
    33601 gggtgataac gctgaagcgg ttaaccagca aatcgtctgg ttcccgccag tgagcattaa
    33661 tctcctgctt acgattttgc tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct
    33721 tccttccggc acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct
    33781 catcaccatc gaactaccgg aatggctgct ggcgatggcg tatatggcaa ttggctggcg
    33841 gattggtctt ggtttcgata aacaaatctt actgcgggca ctacgcccgc taccgcaaat
    33901 cctgctgtcg atttttgctc tgctggctat ttgtgcaggt atggcgtggg gactgacccg
    33961 gtttatgcat attgatttta tgactgccta cctcgccacc agtcccggcg ggcttgatac
    34021 cgtggcggtc atcgccgcag ggagcaatgc cgatatggcg cttattatgg cgatgcaaac
    34081 cctgcgcctg ttcagtattt tgctgacagg gccagccatt gcgcggttta tttcaaccta
    34141 tgcgccgaag cggtcggcct agtgctggca gcctggacac cagtaaaacg ggcgcgagga
    34201 caacgtggtt ttctcaatga tgccgccaca gcgttcgcac agttcgccat cacgatgaaa
    34261 aaccttaaag cgaaacagcg ccccatgatg cttattctca tccacctgcc cccgcgtagc
    34321 gtaagaaaac cgaggaatct ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt
    34381 gagatctttc gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa
    34441 ataattgcca agcccagcca gaaacgcctg atcgagcagt aatcctgcaa actgacggtt
    34501 acgaaagcgc ggcgacaata accgttcctt taccacctcc ggcgtcagat tcggatccag
    34561 cacatcggga ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat
    34621 ctcaatatcc gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg
    34681 caatactcgc gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag
    34741 ctgattatgg ctgtagagcg ttaaaccgtt gggaaaatga gttaacaacg ccttaccacg
    34801 cgtttccaca tgggtaacgt gttgaccgat aagttgtgat tgataagttt ttaactgcgg
    34861 gaaggcaaac cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc
    34921 tgcacggcgg atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaag
    34981 cggggttatg ctgcgacaac aatccccttt ttggcaaatg tagctcgtaa tcggcgggcg
    35041 aacgccagag cgtgctcgcc gtcgccatgc aggcagaccg tttgcgccgc gaccgttgcc
    35101 cattcgccgg tgatactttt gactctgccg tgttgcacca tctccagcgt ttgcgccaac
    35161 gcctgttctt cgttttcaat caacgcgcct ggctgacttc gcggcaccag cgagccgtca
    35221 gcctgataac cgcgatcggc aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct
    35281 gcacgaatca gctcgcttcc tgccagccca acgagaacca atgccggatc gcaagcgtat
    35341 accgctctgg cgatggcgtc tgccagttgt gcttctttcg ccgcctggtt gtacaacatg
    35401 ccgtgcggtt tgacatgacg cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg
    35461 ccaatttgat acagcgtctg ggcgtaaacg gtttccggcg gcagctgcat ggcgctgcga
    35521 ccaaaatttt ccctgtcggg aaaactcggg tgagcgccaa tcgcgacacc attttttatt
    35581 gcttcacgca cgcaagcctg catggtttgc gcatcgcctg catgaaatcc acaagcaata
    35641 ttggcagagg aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg
    35701 gcgttcaggt caattttcat tgtgcagccg ccacgctaat tgttcaaaat aacgttgctg
    35761 atcttgccgc gctttcagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc
    35821 gagcggaatt tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgtggata
    35881 accaccggtg gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gcggcacctg
    35941 caccacgccc ggtaacaaac cgtgagataa cagttcgcga tcggtgatgc gttttaaaat
    36001 ttgcccctgt aaacgatagc ccattcggtt actttgcgag ctaagctgcc agggcgaacg
    36061 ccagaatgca tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg
    36121 aatgcggttg ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcga
    36181 tttgccaatc gggagtcggt caccatcctt cagtaaacgg ccttccagcc cgccaatccc
    36241 cactttgaga tcggtgctgc atgaccccat taccggcgga acatcaatac cacccgcgac
    36301 cgccagataa ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt
    36361 catcggcaat cgccagccgg tccagacggc gttatcatcc aaccgcgctt cgcaacctgc
    36421 acccgtcagg gcaaaccacc catcagtttc gaactcaact gttaactgac cgagcgtgat
    36481 ctccagcgcg ggggcattgg cgtcattacc caccagcagg ttagcaatgc gcagcgcagg
    36541 catatccagt gcgccacagt ggctgatacc cgactggcga aaaccgtgac gaccgccatc
    36601 ctgcacagtg gtgtacatgc ccgcacgaat aatcttcagc atattccctc cttctgcggt
    36661 acaaatcgca cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac
    36721 aggctgagtg aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca
    36781 ccagtctgcg gcccgccgat cccgacagaa cctgccggaa cgagcaagcg cggttcagcg
    36841 cgccgtggcg tgtgttgttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa
    36901 cctaaaaacc agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc
    36961 aacccgcaat acgccgcgac caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc
    37021 ggaatttcaa taaagcgaga ctcaggttcc agggcctcgc tctcctccca ccagcgttgc
    37081 aaacgctcta tggcatccag cgccagcgac tcaggattac gcaaaatcac cgtgatattg
    37141 ttcatgccgg gaatggcttc aaccacattc ggcatatcca ccagacgctg cgccagtcgc
    37201 cagatccgct tctggctagc cagcgtcacc ggcggttcca gttccagcac taccgccgtt
    37261 tcacctatca gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc
    37321 aggattagga atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct
    37381 caatgcgcga ataccaccgc gttcagtggc atggtgacct gcagcataaa aatgcaatcc
    37441 ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac
    37501 gccaaaacgc gcggcgctat cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg
    37561 ctggaccacc tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc
    37621 aatccaggag gctagttcca ttcccggcac aggcatggtc agctctcccc acggcaccaa
    37681 cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc
    37741 cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac aggttgatat cattcgccag
    37801 caacgttttt aaacggttac gcttcattcc gcgaatgacc ggagactctc ctttccagaa
    37861 gtagccatga tgcacaatga ccgcatccgc gcccagacgc actgcctcat cgagcaaagc
    37921 ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ccacctgcaa
    37981 accattcggc gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc
    38041 cagttcggtg tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag
    38101 cgttacctcg cctgttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg
    38161 ccaaaagacg caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag
    38221 acagcatatt gcataaataa tcagttctgt atcaaagttt taactttaag gaaacacaag
    38281 cacgccgcgc aactattacg tgacgttgcc gtcaattgtg gaattattat gaataaacac
    38341 gcatcacaac cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc
    38401 ttttacggca tgcgtgccct gctgattctc tatctcacca atcaactaaa atacaacgat
    38461 actcacgcct acgagttatt tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc
    38521 ggtggctttt tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgttg
    38581 ttgatggcga tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat
    38641 ctgtccctgg cgattatcgt ctgcggctat ggcctgttta aatctaacgt aagctgtctg
    38701 ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat
    38761 gcggcgggta acgtggggtc tattatcgca cctatcgcct gtggttacgc ccaggaagag
    38821 tacagctggg cgatgggctt tggcctggcg gcggttggca tgatcgcggg tctggtcatt
    38881 ttcttatgtg gcaatcgtca tttcactcat acccgcggcg ttaacaaaaa agtactgcgt
    38941 gcgacaaact ttctcctgcc gaactgggga tggctgctgg ttctgctggt ggcaacgcct
    39001 gcactgatta ccgtgctgtt ctggaaagag tggtcggtat acgccttaat tgtcgcgact
    39061 attattggcc tgggtgtact ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgg
    39121 aaggagctgg ggctgattgt gacgctcacc ttattcagta tgttgttctg ggccttcgca
    39181 caacagggcg gtagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatgttt
    39241 ggttataccg ttccgaccgc gatgttccag tcgattaatg ccttcgcagt tatgctgtgc
    39301 ggtgtgttcc tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc
    39361 tggggaaaat ttgctcttgg ccttggcctg atgagcgccg gattctgcat tctgacctta
    39421 agcgcccgct ggtccgcaat gtatggtcac tcttctctgc cactgatggt gttaggcctc
    39481 gcggtgatgg gctttgcgga actgtttatc gacccggttg ccatgtcgca aattacgcgc
    39541 attgaaattc cgggcgtgac cggcgtatta accggcatct acatgctgct ttctggcgcg
    39601 atcgcgaact atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct
    39661 tccggggcga tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg
    39721 ggcgcactgg cgtgtgtagg actggtactg atgatttggc tgtatcaggc gctgaaattc
    39781 agaaaccgcg cgctggcgct ggagtcataa taggccagca ccgatgcctg atgcgacgct
    39841 tgacgcgtct tatcaggcct acgtatttcc tgcaatttat tgaatttgca caaatttgta
    39901 ggctggatta ggcgttcacg ccgcatccgg catctgggct cgattgcctg acgcgtctgt
    39961 tatttcccct tccgcgccgt ctcatacgct gccaacgttt gtactctcgc ctctttgtgc
    40021 tcgactatcg gttgcggata atccagcttc acacctgctt tctgcgccca cttccacggc
    40081 tcatgcaccg ctttccctgg tacatcgcgc agctccggta accatcgacg aataaactcg
    40141 ccctcacggt caaatttctc gccctgagtt ataggattga aaatacgaaa atacggcgca
    40201 gcatcagtac ccgtagaagc cgcccactgc cagccaccgt tattagctgc caaatcacca
    40261 tcaatcagct gcgacataaa atatcgctcg ccttcgcgcc agtcgatcaa caaatctttc
    40321 accagaaaac tggctgtaat catccgtagc cggttatgca tccagccagt gctgttaagc
    40381 tgacgcatag cggcatcaat aatcgggtat cccgttttgc ctttctgcca ggcctgtaaa
    40441 tgtgcgggat tgctttgcca ctgcacacga tccgtccagg caataaatgg acgatgttta
    40501 cacaacgagg ggtaatacgt catcagatga cggtagaact cgcgccagat cagctcacta
    40561 agccagacac taccggcccc accgtccagc gcctgcggct gttccgccaa caagcgatgc
    40621 aaacactggc gaggcgataa cccgcccgtt gccaagctgg cggacaaacg gctggtgcct
    40681 tccactgccg gaaaatctcg ttgttgctca tactctccag caccgttctc gcaaaactgg
    40741 cgtaattgcg caatcgccgc tttttcttcc accgggaaat gcgcagtatc gaaagactga
    40801 cgaggataat tcagcgtaat ggatggcgcg ggctttatcg atccgctact acgaactttt
    40861 ggtgcagcga cgcactccgg catcccttcc cgcagccgtt tcagccaggc attcttaaaa
    40921 ggcgtaaaga ctttgtacat ctcgtgattg ccggtcatca ccgcccccgg cggcaggatc
    40981 acgctgtcat caaatccttc acacaccacg ttacgcagcg ctctttcaac gtccacatcc
    41041 cgcgctcgct cattcacctc atactgatag ttataaaaaa gatgagtaac gctgttttcc
    41101 gcgcacacct gtttaactat ttcgacactg gcgacaaagt catccacttc acggaacaat
    41161 aaaggaatac ctttttccgc cagcgcgatt tgtagcccat tcagttgagc attgataagt
    41221 tcagcctgac gcggcgacat gttatgcgtc tcccactggc gtggtgtagc gatatacagc
    41281 gccagcacgc gtgcagacga attgcggcag gcggcagcca gtgcgagatt atcgtgcaga
    41341 cgtaaatcct ggcgaaacca ggccagatgg gtagtcataa aactcctgaa tggcgcaagt
    41401 taatcacacg cggccaaagt tcgaaataac gctgccctga aagccaggcg tcaggataaa
    41461 gcgccatata gtgtttgata cgcatcagcg gcgcaagaag tggttgctcg ccacggcgat
    41521 aactgtcgat aagcgcagcc agctgctggc gttctgtgga atcaatatgc gggcgaaaat
    41581 aaccctgaac gtgcatcaaa acgttggtgt gatcgcgcgg gttggcggga tgagaaagca
    41641 aaacaattac gcgttggcgg tactggttgt aaaaatcatc cagattttgc cactgatgaa
    41701 tatcagccac aaacgggcca agtttgcggt actcgggctg agaatgggcc agaaagacga
    41761 gtttatagcg gctgtgataa tcgagaagcg ccccgcgcgt taagccatgt tctttcagtc
    41821 gatgtaactc atgaagggcg aagatacgga ttagggtgct gtcatcaaat cgttgtagat
    41881 tcattactgc tcctgaacgg tatacaggag taagtgtaga cgttggtaaa tgatgtttgt
    41941 gaagttaatc cgctagcagg gcaagattac gaaagcgcgc tccccgcaag caactaatca
    42001 aggtagttaa tcatggctga acacgatatt acgccagaag tcaccatttc caaaacacag
    42061 cgccgctata aagtcggcta tgtcagagcc cgccaccagg accgcaaaac cggtatagcc
    42121 cggtattata gcctgcacct taagggcgac tggctgaaag aggccggatt tgataccgga
    42181 cggggtgtca cggtgaagat ttcggagggg ggcctgacca tcatcgctga cagtgacgag
    42241 gtgcaggaac tgcgggagga actttatcag gttaaacagg tagttaaggg gatgcgggat
    42301 gggatgttta gtgtgctgaa cgaaagttaa atattaaaaa agctgggaag atttaaaacc
    42361 ttcctagctt tttattctgt cttattataa tgtatactca tcatcttccg atgaatttat
    42421 aaattgctcc aagtttacat gaagagtact taacctagtt tttaactcat taagtgaagc
    42481 gataatttca tattttccaa cataatcata tgatggatcg tattcattat ctatgtcaga
    42541 agctaaaaat gatgatattc gtagtttaaa tttagcttca tctttattac attcattggc
    42601 ataaggtgaa tgccctatca tagcatattc ctctaatcta aaaggataag atggttttaa
    42661 ttcatcgatc aatgaaattt caatccatgt tctatatcca ggcattaatg gactaatttc
    42721 tttgagcaaa acctttccac ttaataatgt attgagattg ttcattatta tgtctccctc
    42781 gggaaaaata tatgtctaac acttccacca tgttgatgaa cttgtaaatg tggtaaatca
    42841 ccatcaggat ttcctggtcc atgacctgac actctatatg gaggttttgt tacagaagga
    42901 tcttttatat catcccaatg atatgaacct gcttttgttt tatttccagt aagatattcc
    42961 attaagttct tagtgctggg gcctgactca cctgtcatat ttttataatt atgacctaaa
    43021 tatctatcca taaacaattg ttcagcgtca cttctagacc taacagtaac tttggtttca
    43081 ctactatcta ataaacgatt cgctttttgg ttactaacag cacatggagt taatccaagt
    43141 ggatcagcat attttagagg attaggtgca taactgtaga gatttaaacc gcctctcaac
    43201 cctatcgggt cagttaccat gaaccgcccg acatccggat catagtacct gtgcagattg
    43261 tagtgcagcc ccgtttccct gtcgaggtac tgtccctggt agcgcaggtt ctgctccacc
    43321 ccgccgtggt ccttctcctg tatcgtgtta ccccagacct gataccccgt ctcccacacg
    43381 attttcccgt cgctgtccgt catctcttcc ggcgcgccgt tcacatccgt gtgaaaatac
    43441 agtatccggt tcgggccctc tttgcccgcc ttatccaccc gcgccagcgg ttcataactc
    43501 ccctggtccc tgtagatgta caggctgcct gttccctccg gcctgctctc ctgcgccagc
    43561 cgcattccgt cccataaaaa ttttgtccgg ttgtacggct taccgtcagg gctgatgtgc
    43621 tgtttttccg tccgcctccc cagtgcatcg tacacatacc ggtatgtccc ccgcgctgtc
    43681 gtcacttcca ccatccggtg ttccgcatca taacggtaac tctgcgttcc gctccggccc
    43741 tccttttcca ccgcacggcc aaattcgtca taacggtagt gcacaccacg gtaagacatc
    43801 agccggttga acggcaccac gctgttttct gccgtggccc tttcccccgc gcgcctcccc
    43861 agcaggtttc ccgccgcgtc gtactgccag ctcgccagat acccctcatc ctccgaacga
    43921 cacgccgtga tgctccccgt ggggtcgtag aagtaatccg tttccccccg ccggctgtgc
    43981 cgcgtcttca gcagttcatc ctcgccactg tagctgtact gccggtccgt gaacgtctcc
    44041 ggtaccaccc cgcgcatccc gctgtatgtc tccctgcgct tcagccgccc gccggggtcg
    44101 taaagctgcc gcgtgctcag tcgcccctgg cttctgagca cttcacggtg caggtggtcg
    44161 cgcgtgaact cgctgatgac ctcccggtcc agatttatct gctggagatg gccgctgccg
    44221 tagtacagat ggcgctgcgt tctgccgtcc ggaaaggtgg tggcagtccg gttgcccagt
    44281 tcatcgtact ggtgggtcag gatgccctgc gccgtctcct cgctcagcat ccgccccagt
    44341 ctgtcgtagc tgaagcggat aacttccggc gcgtcagttt cgtgccgctg gcgacggatt
    44401 tccagtagct ggtctgccgc atcatggacg tattccgtac ggccatcggc tgtctctttt
    44461 gccgtgagcc ggcccgccgc atcacgcgcc agccggtggc ggatttcacc gccccggtcc
    44521 ccggtgaaca ccacttccgt gaccgcgttc agcgcgttgt agcggtacga ctggcggcag
    44581 ccgtccgggt ttatctgctc cgccagacgg tccagcacat catggcggaa ccagtagcgt
    44641 tctccgttct cgttcgtgag cgtgaccaga cgcccgtaag cgtcgtaccc gaatgccgtc
    44701 ctgcgcccca tcgcgtccgt ccggctgcgc acccggccct gcccgtcccg ctgccagcgc
    44761 gttatcctcc ccatcgggct ggtgtacttt accagcctgc ccgccgcgtc tgactggtaa
    44821 ttcaccgtgc ggccgtccgc cagtatcacc tccgtcagat gaccgctgtc gtcatacccg
    44881 tagcgcgtgc tgtggctctg cgcatccgtc attctgataa gcctcgtgcg ttcgtcgtag
    44941 aaccacgccg tctgcgagcc ggaacagtcc gtgtgccgca tgagctgccc gtcttcattc
    45001 cactggaggt atttattccc cccgcgcgca tctgttatct gcaccacctg gccgtggtgg
    45061 tcatactgat accgggtgtt ctgctgcagc gggtcggtca cggcaagaag gttgccgcgt
    45121 tcatcgtaca cacagcgcca ggtgtttccc gccgcgtcca cctccgtttc cggctggtgc
    45181 cacaccgggt gccactgcgc ctgctccacc cgccccagcg ggtcgtgcgt ctccgtgata
    45241 tgcccctgca ggtcatacac atagcgccat ttgccgccct gagggtcggt catccccagc
    45301 aacagtcgtt cttcgtcgct ccagcggaat gtcgtcacct gtccggcttc gtcctggtac
    45361 gccgtcacct gattctgcgc atcccactgg tgataacgtg tgcgccccag tccgtccgtc
    45421 acgcacagcc tgcgctgcgc caggtcccac gccagccggt actgttcacc gctgtccgtg
    45481 tggtgccccg tcacccgcca tgccgcgtca tccggacctt cataccagcc ccactggtaa
    45541 taataccctt cgccgcccgg ctggcggtgc agcgtcatgc agtggttttc gtcgtaagca
    45601 aactcacgca gcaggtgccc cgtgctgtcg tacacgccgt tcaggtggcc ggcctcgtca
    45661 tagctgtaac gacgcagcag taaaggtgca gtctcaggga aatgctgata aatctgtgtc
    45721 acccgccggg gatgcgccgc cagttcgtaa tacagacgga tgcacggacg ctgctgttcg
    45781 ccgctgattt ccacaatgcg gccccggtcg tcgtagtaca gattcagaca gttgctgtta
    45841 cggtcgccca gcattttcag ccgctggcgc tgcgggtggt gcgggtcagc ctcaaacaga
    45901 aaacactgcc cgtcatcgct gctgataagc cagtgcccct gctcaccacg ccttaccgcc
    45961 agcccttcac cggggctgta aaacccgccc cccggcagta cggcttccat atccaggcgt
    46021 cgccccatgc ccgacacaaa ggtcatgcag ttttcgtctg cgctacccgg tgtgcacacg
    46081 aggctgactt catacattac actccagccc tgaccaaaca ttccttccgt acgttcgtca
    46141 cgactgctgt acacgcgctg ccagtccagc ggaaagtggc ccggcagact gaaatcgaag
    46201 tcatcgtcat cggccaggta tttggccccg gtggcggcat tgaccggatg cggaagggca
    46261 ctggtggcct tctgcgtcag catgctcatc ccgacactgg cagcaaaaca ggcgatttta
    46321 gaaagcattt tgcccgggcg aagaaacatc agcgccacgg ttgccaccag ggttatctgc
    46381 gacctgccgc ttctgatatc cctgaccacc agtaacggac cgccaatgcg cacatccggt
    46441 gacacaaacg ccccgttccc cggctcattc accaccctgg cctcgcaggt acagcgtgcc
    46501 ccgctgcgtg tcgccggctg gctgttgata cgcacatccc gcgcgccttc cgcgatatat
    46561 tccgctgcgg gcggctcgtc atcgcctttg aacagattac tgatgctgcc aataaccgca
    46621 ttcactccct gaaaggcggt ttccagcata ccgcccggat tcagcaggtt aagggccgtt
    46681 cccagggggt catccagaaa ggcggctttt ttttgtgcga gaaactgcgt cagtgaggaa
    46741 ggatgtttat ggcagtcgat tttatcgtca ggggtcgggg tggagcccgg ggccgcactg
    46801 gccacagtgg gctgccatag ttccgacaca aaatgtgtca cgccatccac catttcagaa
    46861 cgcccttctt ccgtactgag ctggtcgtgc agagcccggg acatcagcac aggccctgtc
    46921 accgggttaa ccgccagggc tatggccccc gccatttcca gatattcccc ggccacatcc
    46981 cggaacgttc tggcatcctc ctgacgctgc gcttcttcct gttctgcctt cgcctgcgct
    47041 tcctgcgcct ctatgtcctg ccgcgatgcc gcagtcgccg ctgcccgcgc ggcaggtttg
    47101 ctgttgatcc atgtgtcacc agagccggtg aggatgtaac cttcaatctg aggcgggaaa
    47161 agcatatttg cgagggctga acaggcttcg ctgacctttt ctgtcacgcc ggtgtacacc
    47221 atcgccacac ccgccacaaa accggtcagc acgacggccg tgccccccat cgtgatcccc
    47281 actgacgtcg ccagtaacga ggttgtgccg acaatgaggg catcaataac gacatttgca
    47341 gccacctcca gcaccgcccc cagcacatct gccataaacg acgtatgcag cagggcatcc
    47401 ccctgtcttg ccgcccagaa tgcctcttcc atgtcccgtt ccctgtatga ttaattcgtc
    47461 ctgacttcct gccgcatcct gaagcgcaga ttctttttaa tttcagccca gcgcgtcata
    47521 tctgccgggg tcagcggtgt ggttctggcg taggtgaagg ccatcatgac ccgctcaccc
    47581 ggcaatacaa atgccacctg acactggtac acctgctcgt taccccggag aaactggctc
    47641 tgtatttcca aaccgcctcg ttgagataat attccgtcat aatgtaaata gtccgtttta
    47701 ataaaggaat tatttattga caggttctgt ttttttaatc atgaatgtaa tacccatcac
    47761 cgctttacag cacactttat ctttccagcc ataatcacta ataatattaa gcaattagca
    47821 atatttacaa cgac
//
LOCUS       DAJTYI010000014        47271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYI010000014 DAJTYI010000000
VERSION     DAJTYI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            49..2442
                     /gene="malP"
                     /locus_tag="OO564_000805"
     CDS             49..2442
                     /gene="malP"
                     /locus_tag="OO564_000805"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6652037.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2452..4536
                     /gene="malQ"
                     /locus_tag="OO564_000806"
     CDS             2452..4536
                     /gene="malQ"
                     /locus_tag="OO564_000806"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6652038.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4581..5885)
                     /gene="gntT"
                     /locus_tag="OO564_000807"
     CDS             complement(4581..5885)
                     /gene="gntT"
                     /locus_tag="OO564_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6652039.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6246..6821)
                     /gene="nfuA"
                     /locus_tag="OO564_000808"
     CDS             complement(6246..6821)
                     /gene="nfuA"
                     /locus_tag="OO564_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6652040.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(6880..7563)
                     /gene="gntX"
                     /locus_tag="OO564_000809"
     CDS             complement(6880..7563)
                     /gene="gntX"
                     /locus_tag="OO564_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6652041.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7601..8389
                     /gene="bioH"
                     /locus_tag="OO564_000810"
     CDS             7601..8389
                     /gene="bioH"
                     /locus_tag="OO564_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6652042.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            complement(8390..8755)
                     /locus_tag="OO564_000811"
     CDS             complement(8390..8755)
                     /locus_tag="OO564_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6652043.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(8749..9018)
                     /locus_tag="OO564_000812"
     CDS             complement(8749..9018)
                     /locus_tag="OO564_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6652044.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            complement(9091..9969)
                     /gene="rpnA"
                     /locus_tag="OO564_000813"
     CDS             complement(9091..9969)
                     /gene="rpnA"
                     /locus_tag="OO564_000813"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6652045.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(10172..10408)
                     /gene="feoC"
                     /locus_tag="OO564_000814"
     CDS             complement(10172..10408)
                     /gene="feoC"
                     /locus_tag="OO564_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6652046.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(10408..12729)
                     /gene="feoB"
                     /locus_tag="OO564_000815"
     CDS             complement(10408..12729)
                     /gene="feoB"
                     /locus_tag="OO564_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6652047.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12746..12973)
                     /gene="feoA"
                     /locus_tag="OO564_000816"
     CDS             complement(12746..12973)
                     /gene="feoA"
                     /locus_tag="OO564_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6652048.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(13411..15732)
                     /locus_tag="OO564_000817"
     CDS             complement(13411..15732)
                     /locus_tag="OO564_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6652049.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15829..16305)
                     /gene="greB"
                     /locus_tag="OO564_000818"
     CDS             complement(15829..16305)
                     /gene="greB"
                     /locus_tag="OO564_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6652050.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            16533..17252
                     /gene="ompR"
                     /locus_tag="OO564_000819"
     CDS             16533..17252
                     /gene="ompR"
                     /locus_tag="OO564_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6652051.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            17249..18601
                     /gene="envZ"
                     /locus_tag="OO564_000820"
     CDS             17249..18601
                     /gene="envZ"
                     /locus_tag="OO564_000820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6652052.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18677..20299)
                     /gene="pckA"
                     /locus_tag="OO564_000821"
     CDS             complement(18677..20299)
                     /gene="pckA"
                     /locus_tag="OO564_000821"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6652053.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20678..22402
                     /locus_tag="OO564_000822"
     CDS             20678..22402
                     /locus_tag="OO564_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6652054.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(22537..23415)
                     /gene="hslO"
                     /locus_tag="OO564_000823"
     CDS             complement(22537..23415)
                     /gene="hslO"
                     /locus_tag="OO564_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6652055.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23440..23841)
                     /gene="hslR"
                     /locus_tag="OO564_000824"
     CDS             complement(23440..23841)
                     /gene="hslR"
                     /locus_tag="OO564_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6652056.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23852..24520)
                     /gene="yrfG"
                     /locus_tag="OO564_000825"
     CDS             complement(23852..24520)
                     /gene="yrfG"
                     /locus_tag="OO564_000825"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6652057.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24585..26720)
                     /gene="igaA"
                     /locus_tag="OO564_000826"
     CDS             complement(24585..26720)
                     /gene="igaA"
                     /locus_tag="OO564_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6652058.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            27040..27600
                     /gene="nudE"
                     /locus_tag="OO564_000827"
     CDS             27040..27600
                     /gene="nudE"
                     /locus_tag="OO564_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6652059.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27765..30317)
                     /gene="mrcA"
                     /locus_tag="OO564_000828"
     CDS             complement(27765..30317)
                     /gene="mrcA"
                     /locus_tag="OO564_000828"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6652060.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            30437..31216
                     /gene="hofM"
                     /locus_tag="OO564_000829"
     CDS             30437..31216
                     /gene="hofM"
                     /locus_tag="OO564_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6652061.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            31216..31755
                     /gene="hofN"
                     /locus_tag="OO564_000830"
     CDS             31216..31755
                     /gene="hofN"
                     /locus_tag="OO564_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6652062.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31739..32179
                     /gene="hofO"
                     /locus_tag="OO564_000831"
     CDS             31739..32179
                     /gene="hofO"
                     /locus_tag="OO564_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6652063.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            32169..32573
                     /gene="hofP"
                     /locus_tag="OO564_000832"
     CDS             32169..32573
                     /gene="hofP"
                     /locus_tag="OO564_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6652064.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            32485..33723
                     /gene="hofQ"
                     /locus_tag="OO564_000833"
     CDS             32485..33723
                     /gene="hofQ"
                     /locus_tag="OO564_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6652065.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            34124..34645
                     /gene="aroK"
                     /locus_tag="OO564_000834"
     CDS             34124..34645
                     /gene="aroK"
                     /locus_tag="OO564_000834"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6652066.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34702..35790
                     /gene="aroB"
                     /locus_tag="OO564_000835"
     CDS             34702..35790
                     /gene="aroB"
                     /locus_tag="OO564_000835"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6652067.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            35882..37168
                     /gene="damX"
                     /locus_tag="OO564_000836"
     CDS             35882..37168
                     /gene="damX"
                     /locus_tag="OO564_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6652068.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            37275..38111
                     /gene="dam"
                     /locus_tag="OO564_000837"
     CDS             37275..38111
                     /gene="dam"
                     /locus_tag="OO564_000837"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6652069.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            38129..38806
                     /gene="rpe"
                     /locus_tag="OO564_000838"
     CDS             38129..38806
                     /gene="rpe"
                     /locus_tag="OO564_000838"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6652070.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            38799..39557
                     /gene="gph"
                     /locus_tag="OO564_000839"
     CDS             38799..39557
                     /gene="gph"
                     /locus_tag="OO564_000839"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6652071.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            39550..40554
                     /gene="trpS"
                     /locus_tag="OO564_000840"
     CDS             39550..40554
                     /gene="trpS"
                     /locus_tag="OO564_000840"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6652072.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            40824..41729
                     /locus_tag="OO564_000841"
     CDS             40824..41729
                     /locus_tag="OO564_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652073.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            41746..42108
                     /locus_tag="OO564_000842"
     CDS             41746..42108
                     /locus_tag="OO564_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6652074.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            42192..43355
                     /locus_tag="OO564_000843"
     CDS             42192..43355
                     /locus_tag="OO564_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6652075.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            43356..44582
                     /locus_tag="OO564_000844"
     CDS             43356..44582
                     /locus_tag="OO564_000844"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6652076.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            44579..45457
                     /locus_tag="OO564_000845"
     CDS             44579..45457
                     /locus_tag="OO564_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6652077.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            45468..45759
                     /locus_tag="OO564_000846"
                     /pseudo
     CDS             45468..45759
                     /locus_tag="OO564_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            45771..47075
                     /locus_tag="OO564_000847"
     CDS             45771..47075
                     /locus_tag="OO564_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6652078.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            47087..>47271
                     /locus_tag="OO564_000848"
     CDS             47087..>47271
                     /locus_tag="OO564_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR6652079.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADA"
BASE COUNT        11141 a        11876 c        12951 g        11303 t
ORIGIN      
        1 aaactagcga taacgttgtg ttgaaaatct aagaaaagtg gaactcctat gtcacaacct
       61 atttttaacg ataagcaatt tcaggaagcg ctttcacgtc agtggcagcg ttatggctta
      121 aattctgcgg ctgaaatgac tcctcgccag tggtggctgg cagtgagtga agcactggcc
      181 gaaatgctgc gtgctcagcc attcgccaaa ccggtggcga atcagcgaca tgttaactac
      241 atctcaatgg agtttttgat tggtcgtctg acgggcaaca acctgttgaa tctcggctgg
      301 tatcaggatg tacaggattc gttgaaggct tatgacatca acctgactga cctgctggaa
      361 gaagagatcg acccggcgct gggtaacggt ggtctgggac gtctggcggc gtgcttcctc
      421 gactcaatgg caactgtcgg tcagtctgcg acgggttacg gtctgaacta tcaatatggt
      481 ttgttccgcc agtcttttgt cgatggcaaa caggttgaag cgccggatga ctggcatcgc
      541 agtaactacc cgtggttccg ccacaacgaa gcactggatg tgcaggtagg gattggcggt
      601 aaagtgatga aagacggacg ctgggagccg gagtttacca ttaccggtca agcgtgggat
      661 ctccccgttg tcggctatcg taatggcgtg gcgcagccgc tgcgtctgtg gcaggcgacg
      721 catgcgcatc cgtttgatct aactaaattt aacgacggtg atttcctgcg cgccgaacag
      781 caaggcatca atgcggaaaa actgaccaaa gttctctatc caaacgacaa ccatactgcc
      841 ggtaaaaagc tgcgcctgat gcagcaatac ttccagtgtg cctgttcggt agcggatatt
      901 ttgcgtcgcc atcatctggc ggggcgtaaa ctgcacgaac tggcggatta cgaagttatt
      961 cagctgaacg atacccatcc aactatcgcg attccagaac tgctgcgcgt gctgaccgat
     1021 gagcaccaga tgagctggga tgacgcctgg gccatcacca gcaaaacttt cgcttacacc
     1081 aaccataccc tgatgccaga agcgctggaa cgctgggatg tgaaactggt gaaaggctta
     1141 ctgccgcgcc atatgcagat tattaacgaa attaatactc gctttaaaac gctggtggag
     1201 aaaacctggc cgggcgatga aaaagtgtgg gccaaactgg cggtggtgca cgataaacaa
     1261 gtgcatatgg cgaacctgtg tgtggttggc ggtttcgcgg tgaacggtgt tgcggcgctg
     1321 cactcggatc tggtggtgaa agatctgttc ccggaatatc accagctatg gccgaacaaa
     1381 ttccataacg tcaccaacgg tattacccca cgtcgctgga tcaaacagtg caaccctgca
     1441 ctggcggctc tgttggataa atcactgcaa aaagagtggg ctaacgatct cgatcagctg
     1501 atcaatctgg aaaaattcgc tgatgatgcg aaattccgtc agcaatatcg cgagatcaag
     1561 caggcgaata aagtccgtct ggcggagttt gtgaaagttc gtaccggtat tgagatcaat
     1621 ccacaggcga ttttcgatat tcagatcaaa cgtctgcatg agtacaaacg ccagcacctg
     1681 aatctgctgc atattctggc gttgtacaaa gaaattcgtg aaaacctgca ggctgatcgc
     1741 gtaccgcgcg tcttcctctt cggcgcgaaa gcggcaccgg gctactacct ggcgaagaat
     1801 attatctttg cgatcaacaa agtggctgac gtgatcaaca acgatccact ggttggcgat
     1861 aagttgaagg tggtgttcct gccggattat tgcgtttcgg cggcggaaaa actgatcccg
     1921 gcggcggata tctccgaaca aatttcgact gcaggtaaag aagcttccgg taccggcaat
     1981 atgaaactgg cgctcaatgg tgcgcttact gtcggtacgc tggatggtgc gaacgttgaa
     2041 atcgccgaga aagtcggtga agaaaatatc tttatttttg gtcataccgt ggaacaagtg
     2101 aaggctattc tggccaaagg ctacgacccg gtgaaatggc ggaagaaaga taaggtgctg
     2161 gacgcagtat tgaaagagct ggaaagcggt aaatacagcg acggcgataa gcatgccttc
     2221 gaccagatgc tgcacagtat cggcaaacag ggcggcgatc cgtatctggt gatggcggat
     2281 ttcgcagcct atgttgaggc acaaaagcag gtggatgtgc tgtaccgcga ccaggaggcc
     2341 tggactcgcg cggcgatcct caataccgcc cgctgcggta tgtttagctc ggatcgctct
     2401 attcgcgatt atcaggctcg tatctggcag gcaaaacgct aaggaagctc gatggaaagc
     2461 aaacgtctgg ataatgccgc gctggcggcg gggattagcc ccaattacat caatgcccac
     2521 ggtaaaccgc agtcgattag cgccgaaacc aaacggcgtt tgcttgacgc gatgcatcaa
     2581 cgtaccgcca cgaaagtggc ggtaacgcca gtcccgaatg tcatggttta taccagcggc
     2641 aaaaaaatgc cgatggtggt ggagggcagc ggcgaatata gctggcagct gaccaccgaa
     2701 gaaggaacgc agtacaaagg ccatgtaacg gggggcaaag cgttcaatct accgacgaag
     2761 ctgccggaag gttatcacac gctaacactc acccaggacg accagcgcgc gcattgccgg
     2821 gtgattgtcg ccccgaaacg ttgttacgaa ccgcaggctt tgctgaataa acaaaagctg
     2881 tggggtgcct gcgttcagct ttatacgctg cgatcggaaa aaaactgggg tattggggat
     2941 tttggcgatc tcaaagcgat gctggtggat gtggcaaaac gtggcgggtc gttcattggc
     3001 ctgaacccga ttcatgcgct ctatccggca aatccggaga gcgccagccc atacagcccg
     3061 tcttctcgcc gttggctgaa tgtgatttat atcgacgtta acgccgttga agatttccat
     3121 cttagcgaag aggctcaggc ctggtggcag ttgccgacca cgcaacagac gctgcaacag
     3181 gcgcgcgatg ccgactgggt cgattactcc acggttaccg ccctaaaaat gacagcatta
     3241 cgaatggcgt ggaaaggttt cgcgcaacgt gatgatgagc agatggccgc gtttcgccag
     3301 tttgttgcag agcagggcga cagcctgttc tggcaggcag cctttgatgc gctacatgcc
     3361 cagcaagtga aagaggacga aatgcgctgg ggctggcctg catggccaga gatgtataag
     3421 aacgtggatt caccagaagt gcgtcagttc tgcgaagaac atcgtgatga cgtcgatttt
     3481 tatctctggt tgcagtggct ggcttacagc cagtttgccg cctgctggga gataagccag
     3541 ggctatgaaa tgccgattgg cttgtatcgt gatctggcgg ttggcgtagc ggaaggtggg
     3601 gcggaaacct ggtgtgaccg tgaactatat tgcctgaaag catcggttgg cgcgccgccg
     3661 gatatcctcg gcccgttggg gcagaactgg ggattaccgc caatggaccc gcatatcatc
     3721 accgcgcgtg cctatgaacc gtttatcgag ctgttgcgtg ccaatatgca aaactgcggc
     3781 gcattacgaa ttgaccatgt gatgtcgatg ctgcgtttgt ggtggatacc gtatggcgag
     3841 acggcagatc agggcgcgta tgttcactat ccggtggatg atctgctctc gattctggca
     3901 ctcgaaagta aacgtcatcg ctgtatggtg attggtgaag atctcggtac cgtaccggta
     3961 gagattgtcg gtaagctgcg cagcagcggt gtgtactctt acaaagtgct ctatttcgaa
     4021 aacgaccacg agaagacgtt ccgtgcaccg aaagcgtatc cggagcagtc gatggcggtt
     4081 gcggcgacac atgacctgcc aacgctgcgt ggttactggg agagcgggga tctaacgctg
     4141 ggcaaaaccc tggggctgta tccggatgaa gtggtactgc gcggtctgta tcaggatcgc
     4201 gaactggcga agcaagggct gctggatgca ctgcataaat atggttgtct gccaaaacgt
     4261 gccgggcata aggcatcgtt gatgtcgatg acgacgacgc tgaaccgtgg tttgcagcgc
     4321 tacattgccg acagtaacag tgctctgtta ggactacagc cggaagactg gctggatatg
     4381 gccgaaccgg tgaatattcc tggcaccagt taccagtata aaaactggcg acgcaagctt
     4441 tccgcaacgc ttgagtcgat gtttgccgat gatggcgtga acaagttgct gaaggatttg
     4501 gacagacggc gcagagctgc agcgaagaag aagtagagtg cggttgatgt cggatgcgat
     4561 gcatccggca gtcagtgtta tcaaatcacc atattcagca gcagacagcc taccagaccg
     4621 cacaccgaga taatggtttc cagcatcgac caggatttga tggtctcgcc gatagtcagg
     4681 ttaaagtact ctttgaacag ccagaagccc ggatcgttca catgagagaa aatcacacta
     4741 ccggaaccca ccgcaataac catcagctct gggctaacac ccgtcgttgc aatcagcggt
     4801 gccgcgatac cacccgcagt gattgccgca acggttgcgg aacccagcgc gatacgcagt
     4861 acggcagcaa tcgaccaggc catcagcagc ggagaaatgt tggtttcgtg catcatggaa
     4921 gcaatgtatt tgtccacgcc gctgtctacc agcacctgct tgaacgcacc gccaccaccg
     4981 atgatcaaca gcatcatcgc aatgattttg atggaagaaa ccagcgtgtc gttaatctga
     5041 tccattgaac gaccacggtt cagaccaaag gtgaacatcg caatcagcac ggcaatcagc
     5101 gttgccatta ccgggtcacc gaggaactcc gctaccggca ggaaagcgtg acctttcggc
     5161 aggatcattt cggcaatcgc acgcatcgcc atcagtacaa ccggcaccag agaggtccag
     5221 acgctgacgc caaagctcgg catctcttct tcgctgaagg ttttcgcgct gtagagacct
     5281 tccgggattg gcttatcgat acctttcaac acgcgagcgt aaaccggacc ggcgagaatc
     5341 acggtcggga ttgccagaat agtaccgtac agcagggttt tacccatatc ggcattgaaa
     5401 atggtggcaa tcgcagtcgg gcccggatgc ggtggcagga agccgtgggt cacagacagt
     5461 gcagccgcca ttggtacacc aacatacagc aatggaatat tcgcggaagc cgcgatggta
     5521 aacaccagcg gcagcatcag cacgaagccc acttcataga acagggcaaa accaacggta
     5581 aaaccggtta acaccaccgc ccactggatg tgctttttac caaatttggc aatcagcgtg
     5641 gtggcgatac gttgtgcgcc accgcagttt gccagcattg cgccaaaacc catgatcagg
     5701 gcaaggctac cgagcgtccc gccgacaccg gctttgatgg agccaataac tttatccagc
     5761 ggcattcctt gcattaatcc aacagcaagc gccacgagga cgagagcgat gaagccgttc
     5821 attttgaagc ggatcatcag gagcaacaac aagattacac cgatagcaac aatgactaat
     5881 ggcatgattt acctggcctt tcatttgtta tgggtaatgt caattttctg acgacaaact
     5941 ctaattatcc caatcgggaa cagagatatt gcggcaccac gactgatacc cactaaaact
     6001 aattattgta gtcagatgtc aggagtatgt ttggtaccca tgtgaatgat acgggtaaca
     6061 tctggcgttt gagaatcacc agagcggggt aaatttaaat tatgagaggt tggtcatatt
     6121 atcgcgggga aacgaaggga gggatttgcc aaagcaatgc tgtgccaacg tctggcacat
     6181 gttcaacgta ggcccgaaat gacgctttag cgtcgcatcg ggcaatctac aaaagagggg
     6241 ataacttagt agtaggagtg ttcgccgcgc tggtgttcgg tgagatcgcg cacacctttc
     6301 agctccggga attcgttcag cagctgcttc tcgatccctt ctttcagcgt cacatcgacc
     6361 atggaacaac cgttacagcc gccgccaaat tgcagaatgg cgtaaccgtc ttcggtgatt
     6421 tccatcagcg aaacgcgacc accgtgacca gcaagctgtg ggttgatctg cgactgcagc
     6481 atatactcca cgcgctccat cagcggtgca tcgtctgcca ctttacgcat tttggcgttc
     6541 ggggctttca gcgttaactg agaacccaac tggtcggtaa caaagtcgat ctctgcatct
     6601 tccaggtatg gtgcgcttaa ctcatcaaca tacgcggtca gcaggtcaaa tttcagggca
     6661 gtgtcggtgg cttccacggc atccggcgga caataagaaa caccacattc agcgttaggc
     6721 gtgccagggt taatcacaaa tacgcggatt tgtgtccctt cttcctgatt tgccagcagt
     6781 ttggcaaagt gcgcttgtgc agcatcggaa atacggatca tagtaatggc ctaatagttg
     6841 actattttag ttggttataa tacgcccatc atcgaggctc tacaaggttc gacaaaggca
     6901 ccagacctgg acagtcgccg caccattgcg taaaagcaac tgcgcaatct ctgcgacggt
     6961 acttccggtg gtaacgacat catccacaat caccatatgg cgaccttgca cgggcaattc
     7021 aagacgaaag gcatttttca ggttgcgctt gcgcagccgg gcactgagaa aatgctgggt
     7081 cgcagtggcc cgtgtacgtg tgacggcttc gctatcccat tggcagtgca accagcgtga
     7141 taacggctga cacagcaaat cgctctgatt aaatccccga cgccagtgac gccgctgcca
     7201 taacggaacg ctgatgatgc gatccggcaa ttgcaacccg gtggtgcgac gagcgtgtaa
     7261 gacttccaat agtaacagac gtgacagggc gctggcgatt tcactgcgcc gggaaaattt
     7321 aagctggtgg ataagcggac ttaacggcgg cgcatagtcg gcaaccgtga ccagtctttg
     7381 ccagggcggc ggtttttgca ggcagcgacc gcagggaaga tgggagtgtg tggcgggtaa
     7441 tccacattgt gggcataacg ttttatctgt gcgggtggcg cgtgaacaga ccgaacaaat
     7501 cccccaatga cctaacgcca gtggcattcg gcatagccag cataatcccg gtactgttag
     7561 catatgttca tccttgtaag tcaaaagaga acaatagcgg atgaataaca tctggtggca
     7621 gaccaaaggt caggggaatg ttcatcttgt gctgctgcac ggatggggac tgaatgccga
     7681 agtgtggcgt tgcattgacg aggaacttag ctcgcatttt acgctgcacc ttgttgacct
     7741 gcccggcttc gggcgtagcc ggggatttgg tgcgctgtca cttgctgata tggccgaagc
     7801 cgtgctgcaa caggcacctg ataaagccat ttggttaggc tggagtctgg gcgggctggt
     7861 ggcaagccag attgcgttaa cccatcccga gcgtgttcag gcgctggtca ccgtggcgtc
     7921 gtcaccttat tttagtgctc gtgacgagtg gccggggata aaaccggacg tgctggcggg
     7981 atttcagcag caactcagtg atgattttca gcgtacagtg gagcggttcc tggcgttaca
     8041 aaccatgggg actgaaacgg cgcgccagga tgcgcgggcg ttgaagaaaa ccgttctggc
     8101 gttaccgatg ccggaggttg acgtgcttaa tggcgggctg gaaatcctga aaacggtcga
     8161 tctccgtcag ccgctgcaaa acgtgtccat gccgtttttg cgattgtatg gctatctcga
     8221 cggtctggtg ccgcgcaaag tggtgccgat gctggataaa ctttggcctc acagcgaatc
     8281 atatatcttc gccaaagcgg cccatgcgcc gtttatttcg catccggtcg agttttgtca
     8341 cctgctggtg gcgttgaagc agagggtgtt ggtggtaagt gagagctaat tattgtgggt
     8401 cgattaaatt caccagttta ctgataactt cactaatcaa tttttcatcg gcaacggtat
     8461 gaaatttagc gccacgggca ataagatcga cggcttttag ctggtgacaa agtacaacgc
     8521 catgcaggtt accggtttgc gtatcacagg ggaggacgtt caccgtcacc cctgtggaac
     8581 gtgctgcatt tgcccctgtt gaaatcgggc agcacatagc aacttttaaa acattgttga
     8641 gttttttgtc cgttaccaca atgcaataat gtggcccctg aagttcatgc ccggcaaccg
     8701 gatcagggtt aaaataccag atctcaccac gatgtggcgt ccgtgccttt accatgcttc
     8761 attacctttt ttcccttcag cccatccttc tgtggactga tttagctccg taatttcgct
     8821 ttcatcgatt tggctcaaca actgcgcgac ggtaagacgc ccacgcggtt tgtgctctgt
     8881 ggcgcgcaaa ttaacgctat cgcctttttt ctccacggta agctctgtgc ccggtgacca
     8941 gccaagatcg atggcaagat ctcgtgggat agtaacgatc atggctcctc cttgttgccg
     9001 taatttcact gtctgcatat cagttcccta agtgctactt tgtgttacat gcaagatagc
     9061 acaaagtagc acttaagcag ggtagagtat ctactgactc gccgcagcca actcttcttc
     9121 tgacagcccg gtaaatcgca tgatgtctgc aagagggact ccggattcaa gcattatttt
     9181 ggctatatgc agggctttgg attgttcccc ctcctgccgc aatctttccg caatagtcat
     9241 taaactctcc ttgtgtttcg gtgaacgttc ggcaacgccg tcgataaaat cgttaaaacg
     9301 tacagcgtcg ccagtttgca gtatgtaatt aaacagccct ttgatttgtc tgtcattagc
     9361 gtatccacta cttaataagc aggccatttg ctctaccagc cccatcagat cgcgttgacg
     9421 aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga tttcatcatc
     9481 aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc ttgccagttt
     9541 gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt caataccgtg
     9601 gtaaaacaac aatggcacca ccatcggcaa cgttttgtat ccagcatcaa ggtgattttg
     9661 cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg aggtgctttg
     9721 atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg accacagcac
     9781 atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca gcttgagggt
     9841 tttcatatca cagagcgcgt gaatgggtgc cggtaaatga aacgcaagaa aatcgcgtgc
     9901 cgtctccggt tggcgtaaaa agagtttgaa cagcgcatca tgtggggtgg aactctgctt
     9961 tttactcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc gccactccac
    10021 atctgtgcat tagcgagttt tcatccctgc gagacgcttt cagaggaact tacgttgcgc
    10081 tgcaagaatt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc aaacgcatac
    10141 cgggcttttc aaaacaggcg atggagtatg gttaacgcag cgcccaccac tcgcgcagac
    10201 aggctttgcc ttccgggcag cttttacaac tgccagagag gcagccgtca ggttcttcct
    10261 gaatccgcac ggctttgccc atgctttcca gctgttgcag catggcgtta atcattggct
    10321 gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt aacgccagca
    10381 aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc tggctgcgca
    10441 gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc tacgcgcgcg
    10501 gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc acaccaggct
    10561 gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca gtgagtaagc
    10621 gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt cacgggcgat
    10681 ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt agctgtatgc
    10741 ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac ccatttcacc
    10801 gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg tgtctttcag
    10861 gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga attccgccgg
    10921 attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg tacccaccac
    10981 cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt tatcttcatg
    11041 tacgccaatc ggcttgaaga caggggttat cacccggctg acggaagcca gcgccgagtc
    11101 gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc tcaggaaaat
    11161 gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac gctgccaggt
    11221 ctggataatc aggcttttaa cgtgtggtac atggtatacc ggcagctcca tgacaaacgg
    11281 cgttgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca ccgccatcac
    11341 gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga agaaggcagc
    11401 cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg ccatcatgat
    11461 ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg acggtacgtt
    11521 acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca gcgcctgcat
    11581 caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa ggaaggagag
    11641 gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa taccgccacc
    11701 catgccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt agccaatcca
    11761 ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct gtaacgcccc
    11821 gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa tcggcagacc
    11881 gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg gttctgccgt
    11941 cagggtgttg cttaccacat cacagatggc agcaatgcac tggtaacgcg catcggcaat
    12001 gtgcagcgcc ggatcatcca tttcattacg cagacgggcg agggtggcat ccagatgctg
    12061 cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt gcaggcccag
    12121 ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg aatctgcttc
    12181 gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt tatagcgatc
    12241 aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg ggatcaccgg
    12301 gcagccaaga cgtgccgata gagcatcaat ttcaatacga atattttgct tctcggcaat
    12361 gtcgagcatg ttcagcgcca cgatgcaggg aatgccgagt tccagcagtt gcagcgtcag
    12421 gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt cggcgtcgcc
    12481 actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg agatggtggt
    12541 cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg tggagaattg
    12601 cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac gtgcgccagt
    12661 gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc caatggttaa
    12721 ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct aataaggcca
    12781 gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg gggtcgccga
    12841 gtggagcgac gcgcaccaca tcaaaagagg agccaggtaa catgcctaga gaaagcagtt
    12901 tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac gcagtatctg
    12961 gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc tcatggggcg
    13021 tttgaccgcg acatgagacc agatgatttg ccaacaaagg agagattaaa taatcaacaa
    13081 cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtacg aatgttggtt
    13141 tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc gaaaatataa
    13201 ttcgaatagt gagttacccg gactggctaa tgtttaatta aggtttcagt tgttaatgaa
    13261 gtgataaata ttaacggagt atattgtgag tttggctgtg gaataaaagt gccggagata
    13321 ttctccggca cttttatgtc aggcaaagcg ttcgttgcca gatgcggcgt gaacgcctta
    13381 tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttacccatcg ctgccgccag
    13441 cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg gtttggctgc
    13501 cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgagagttgg tttcgccagg
    13561 ctgctcgtcc agacgcatag tcagggcgat acgtttacgc tgaagatcca cttccagcac
    13621 tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca caaacttgtt
    13681 cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga caaacgcgcc
    13741 aaagttggtg acgttggtca ctgcgccttc gaggatcata cccggttgca ggtcgttcat
    13801 tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat cgcgacccgg
    13861 tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt catcagtaaa
    13921 gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt tcagtgcctg
    13981 ctgtgttgct gccagaatgc gttccaccac cggataggct tccggatgaa cggtagaggc
    14041 gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct cgaaggcttt
    14101 cggccccaga cggctgactt tcagcagttg ctgacggttc tggaactggc cgttctcatc
    14161 gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc gggttaatag
    14221 cggaacagaa gcggtgttga gatcgacgcc aacgccgttt acgcagtctt ctactactgc
    14281 atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac ctacgccgat
    14341 agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc gggcgataga
    14401 taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca gctcggaagc
    14461 tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca ctttcgggaa
    14521 ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac cgttgccgat
    14581 cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg ctgcttttgc
    14641 ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag tggcatcgac
    14701 caccgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg cacgcagtcc
    14761 ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga ttgcttcatc
    14821 ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca gcaccttgat
    14881 gcgccaggtc cagctcacta cgcttttgcg ccagctatcc gccggggcat tgttcaggcg
    14941 caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg gctcatcgaa
    15001 ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac ggaacatcgc
    15061 cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat agtcgcggaa
    15121 tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg cgttcttcca
    15181 cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt ccatcaggat
    15241 atagcgcgcg ccatccagcg cggctttggt atctgccacg cctttatcgg catcaacata
    15301 ttgtgcagcg gcgacttctg gcgtgtgtga cggatcgctc cacagcaggt cagccaacgg
    15361 ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt tgtagggcag
    15421 gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg ccagatcatc
    15481 ggtgagtttg ccttgctcgg aaatggattt gaggatcgcc tgacgtctct cttccagctc
    15541 gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca gaccgccggt
    15601 gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcatcaa gcaggcgaac
    15661 ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc ggcagaacga
    15721 atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat agttatacgg
    15781 actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcctt acggtttcac
    15841 gtactcgata gcattaacat accagttcgc ttccccggca ggggtattca ccaccgccag
    15901 atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag agatgtaatc
    15961 tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca cgccatcgtc
    16021 gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag gggagtaatc
    16081 gacaattttg agattttcca ggcatttagt gagatagcgc acgcgacggt cgatttcacg
    16141 cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca gacttgcggc
    16201 ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat taagctcttg
    16261 tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt tgattccctt
    16321 tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga atcaataatg
    16381 tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa caatttaggc
    16441 tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa tacacgctta
    16501 caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag attctggtgg
    16561 tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa caaggcttcc
    16621 aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt gaatctttcc
    16681 atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt tgccgacgtc
    16741 ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg gaagaagtgg
    16801 accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg tttaacccgc
    16861 gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa ctgccaggcg
    16921 caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac ctcggtacgc
    16981 gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt gcggtactga
    17041 aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg aaccttgccc
    17101 gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg cgtctgcgcc
    17161 gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg ggcctgggct
    17221 acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc cacgaagttc
    17281 atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg tgacgactta
    17341 tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata aagtcctcgc
    17401 gtacgaagtg cgtatgttga tgaccgataa actgcaactg gaggacggca cgcagttggt
    17461 tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc tctactccaa
    17521 cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct taagccatca
    17581 gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca aaagttcgcc
    17641 tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc cgctgaccga
    17701 aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta tgctattggc
    17761 gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg atctcgaaca
    17821 cgcagccttg caggttggta aagggattat tccgccgccg ctgcgtgaat atggcgcttc
    17881 ggaggtgcgt tccgttaccc gtgcctttaa ccatatggcg gctggtgtta agcaactggc
    17941 ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc cgctgacgcg
    18001 tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag aatcgatcaa
    18061 taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc tgcgcaccgg
    18121 gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg tgattgctgc
    18181 cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca ttgaagtgaa
    18241 aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg ccgcccgtta
    18301 tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct ggttccaggt
    18361 ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc agccgtttgt
    18421 ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa ttgtgcagcg
    18481 tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg gcgggctttc
    18541 cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa aagaagggta
    18601 aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg gatagatatt
    18661 ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca cccgcagggg
    18721 tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg gctttttcct
    18781 gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg tctacgcccg
    18841 gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct gcattatcca
    18901 gtgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag atacgtttgc
    18961 cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc tgcatacgtt
    19021 tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg ccgaagcaag
    19081 cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt ttggcggtga
    19141 agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc ggcggcaaca
    19201 cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct ttggaaaccg
    19261 gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc tcggtttttg
    19321 aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc agcagcgcat
    19381 cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg atagtttttg
    19441 cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt tcgtcatcgc
    19501 caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg gaaaggccga
    19561 agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata gaagcgatac
    19621 ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt tcgccgccgt
    19681 accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg aagttttcgg
    19741 agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc ataacgataa
    19801 agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg tttttgacaa
    19861 aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta tccgggttcg
    19921 caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg gtcaccaggc
    19981 ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta cctttgcctt
    20041 tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac ttatcttttg
    20101 gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta gttaacaccc
    20161 cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg tcgtagcttg
    20221 ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct tgcggggtca
    20281 aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct gaatagtaaa
    20341 gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg gagaaattca
    20401 tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc gcagaaatag
    20461 cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg gcgggaatat
    20521 tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta aagtatcgat
    20581 tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac tgcccggatg
    20641 cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg aactttcacc
    20701 ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt gctacctgct
    20761 tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc ctgcgaccgt
    20821 cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc gcctttgggg
    20881 atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga agtggcaaac
    20941 tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct gctatctgct
    21001 gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc gcaatggcag
    21061 tagccgctat agctactttt accagtcagt atggcataac gtacttatat tactggtgat
    21121 ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt tgtttaaact
    21181 tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta tgtatcttac
    21241 atttggtctg gttaccgcac tggcggtgat cctcgcgcgt acacagtcac gtttaatcga
    21301 ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag tatcattgct
    21361 aaccctgatg tttatcatca ccctgccgtt tacgggcctg agcgcgattt ctcgccacat
    21421 ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa tggctattgt
    21481 ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc tgattaaaac
    21541 cgctttgctg gttgctccgc tgtatgtgtt cgtcgccacc tgggggttat ggctgcgggt
    21601 cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggagc tggtgttact
    21661 ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa atccccttgt
    21721 tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac tggtgcttct
    21781 taattcgccg gtgctggaca gtatgcgtat tagcgtgaac agtcatatgg cgcgttatca
    21841 gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga gcggtcgcta
    21901 tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag acccgaaacg
    21961 cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac aggtatcgga
    22021 aaaagtatta gccgaaaatg tgttaattgc ccctggttct gttaaaccgg atgcgacatt
    22081 ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa aagacgcctg
    22141 cgtactggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc tgtttgcttt
    22201 taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat gggacgcgct
    22261 tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag ctgcgaagga
    22321 tggaaaactg gggacgaggc cgaaagcgtg gcgcgatctg acggtagatg gtgaaacgct
    22381 ggaaataaac ttaagtaagt gacaacgctt ttatcggata cgacgctgta tctgaccaaa
    22441 ataatttgcg atttatttca taggcgctgg taagcgcagc acatcaggcc attaagtcgt
    22501 aaaatccggc agagccctct ctgccggaca tactcattaa tgaacttgcg gatctgccgg
    22561 agacgcgttg ttgcggattt cagcaatatc catcgcattg aacagatagt ggttaccgca
    22621 gtaatcacaa tgcatgtcaa tttcgccatc ttccgccagg atgctatcaa cttcttcatc
    22681 aggcagcgtt ttcagcgcat cggcgcaacg ttcacgcgag caggtgcatt tgaactccac
    22741 atcctgcgga tcgtaaaccg tcacctcttc ttcgtgatac aaacgccaca acacttcgtt
    22801 tgccggtaag gtcagcagtt cttcggtttt gatggtttcg gttagcgtcg ccaggtggtc
    22861 aaagtcgtcc tgctgggtat tttgcgcagg cattacctgc aacaacatac cgcctgcagc
    22921 cggtttgccg tctacgtcgc cggtgcgaat aaacaggcgc gtcggcagct gttcagaacg
    22981 cataaagtaa tcttctaggc aggccgccag ggtatcacct tccagaccaa ctacgccctg
    23041 atagcgttcg ccttcgctcg gggtaatggt gatcaccacg taaccattgc cgaccagcgt
    23101 tttcaggtcg gcattttctg gaatttcgcc ctgcacgcgc gccacaccgc gcatttgctg
    23161 gttattgtta ccgttaataa ccgccagatt catcggaccg tcgccctgca gctgtacggt
    23221 gatatcacca tcaaacttca gcgtagcggt taacaggctg gtcgcaacca gcagttctgc
    23281 cagcacgttt ttaacgggct gcggataatc gtggttctca aggatctgtt gcagggtttc
    23341 cgaaacggtt accagttcgc cgcgaacggc aaaattttca aacagatagc gatgtaattg
    23401 gtcatgttgc ggcataatca tctctcttgc aggtgacagt tattcactgt cgccgtgttt
    23461 aaatcgtaac aggtcgcggc gctctttttt gtccgggcgt cggtccgggt gcggcatggt
    23521 taaggcatta agtttacgtg ccatcgccat tttttcgcgt ttctctacac tttccgcagt
    23581 ctcttcatac agcaaggctg cctcgctggc ggggcgacgc tgttcagtaa tcgcctttac
    23641 aatcaccgtg cgttcgtcat ttccctggcg cagagtgagc gtggcattca gctcgacgat
    23701 tttgctcggc ttgctgcgct gcccgttgta atgcacctta ccgcctttaa tcatttcacg
    23761 ggccagcgcg cgggttttat aaaaacgggc agcccatagc catttatcca gtcgaacctc
    23821 aacagcaggt ttctctttca tggcgtctcc ttcacattag cgaggggatc aggcggcggt
    23881 agtcattcag tgacggatgg cgttgatact gtttctcggc aatcccggaa tcaggattag
    23941 tcacgccgag gcagtaacga ataccaaatt gcgcggcagc atcgagaatc gcttcgctgt
    24001 catcaataaa cagcgttctt tcagctttca gacccgtagc ttcggccacc gcatgccata
    24061 accgctgatc ctctttcgga taaccaaatg tgtgggtgga aagtaataaa tcaaggtgtg
    24121 cgtccagacc cgtatgctca agttttaccg ccaggttgtg cggatgagca ttggtgagca
    24181 aaattcgctg cttaccgctg gctttcagtg cctcaagaaa cggaatggta tcttcacgca
    24241 gtacggcgcg cggtcccatc tcggtggtca tcgcacagat atccagaccc agttgctcac
    24301 tccagtaatc aagacagtac cagtttagcg tatgctgtac gtcgtgatat tgctggcgca
    24361 tatattccat cgcttcctgt ggcgtaaccc cgtttttcgc gccccatgtt tcaggcacca
    24421 gcttttgcca gaaatagtta tcgaaggcga ggtcgagcaa cgtgccgtcc atatccagca
    24481 gaacggtatc tacgtcctgc caggcaatgt tgatatgcat gagggaaatc tccagagtga
    24541 agcaatttgc gcgacagggt agcataacct gccgcgcaaa cgtgttattc gataaggctt
    24601 tctgaagggg tgatcagttg cgggttcagg cagctttcat aataggcctg aatctccatc
    24661 atgcgagtgc gatgacgctg gtaacgacgc caggcctgta cgccattgta aatggcgcta
    24721 cccagcatcg tcagtagcag caatgtggtg ctgagatagc gccacaggcc ggaacgatcc
    24781 gggatcggat gaaggccaat atgctgcgta ccattggcgt cagtgaagat ttttgtgacg
    24841 ataccttcgg cgttaaacgg cgtatgcatc agcatttgtg ccagtttctg gaaagcgttc
    24901 cactgttctt gcggcgggta gtcgtaaagt gatgccgaag gccagggctg atcaacaaaa
    24961 tcgctgcctt cgtcgctgac aatcaggaat ccgccggggg ccggactgtt tagtgattgt
    25021 gccgctcgcg ccgtttcatg cgtgataaac ggcgcggtgg aggttgccac caggttatcc
    25081 agcgattccg cactcaccgg gcgtaataac acattcacgc catcgagctt cccagcgttg
    25141 gcgcgtttta ccagcgcgtc ccagtcttta ctgttgccga gattgaccag cgcatttttc
    25201 agtcgcacac agtcatcttt ggcagaacat aaatccgctg tcttcagtac aatgtcgcca
    25261 aaatcatcaa gcaataccat gccggatttt tgaatcgctg aacgtaatga ggcactgacg
    25321 cgagattcat cttccggttt agggtgcagc tggcgattaa ctgcttcagt caatgccgtc
    25381 gctttgttga ccagttcaga ttctggtaat ggtaatgagc gggcgtcatt ccagatgatc
    25441 tgcgagcagt caaatggtaa aaaaggtgaa ttggttttcg cgctccaggt tccggaagtg
    25501 cgaatattac acattcccgt accgctaata cgcaatgtat cgcctacccg cacgccagcg
    25561 tcagccagtt gttttacgct ggtggcttca atggtctgcg cgcctttcat ccatgagaga
    25621 gtgaatttca gcggcatatc cagcgggatc cagaataaca gcataaacag caccagcagc
    25681 gagcccgcag cgataatcgt actgcgcagc cagtgctgta acggaaagtt ttttacttca
    25741 tcatgcagcg aaagatatcg cccctgacgc actacgtggc ggtcgagata gatatcaata
    25801 tcggtttgtt gaccgagatc ctgagcaatg tatggctgcc agtgtgcggg atagaccagg
    25861 tcgataatac cgagtgaaat attgttgatc tgttcctgat cgttttcgcc aaacaatccc
    25921 caacgacggg gtgtaccgcg cagacaatga atttctcgca gggaggattt tgccgggggc
    25981 gcgaatagcc cccacagacc tgcgcccagc agcagtaatg cgccgcctgc cagccacgga
    26041 acaaatacat ccggggtaat caggcagaaa aagaacatca ggaaggaggc gacgatcagc
    26101 aacgcttcac gcagcccgcg cggacgactc agcgcatatt cttcatgcgt ttctttgcga
    26161 atattgagta gctcgatttg ctcactctct tccccgcgaa tcgacgcctg cgtggacgga
    26221 acaggttgca aggcatagcc gcgcgtttcc tgcatgtact cctgcagcgt atgaccgttg
    26281 agtgaaataa ccaacggcag cgaatcggta tgaatcagtt caacggtatt ctcatcgttg
    26341 atgtactgtt cccagaaggg gggcaggtgg acttctaccg aatcgaggta gtaacgccat
    26401 ttattcggat cgtcggtaga gatgccgtaa cgtgtgatag cgtgtgtcag catcatgacg
    26461 ttgttgcttt cggcattcag cgccagagag atcggtgccg cgctagctcc cgttgggcca
    26521 ggcacctgta atacctgcgt caggctctca agataatttt caacggcgct acgttcttca
    26581 ggtgtgagtt tacgcgtttg cgcatccgcg aaggcgttgg tccagggcag ctgacgccgt
    26641 ctggatcgca cttttatcag ccatcccgca agtagtgagc aggccagcaa agcagctaaa
    26701 aaaatcacaa tggtgctcat gctttcccca tcttacttag tctgtcaggc gtggtcagtc
    26761 gcaccggaca caatacatga ttctgcggtt ggctatcggc aagcgtggag tttaacttga
    26821 gcatcattaa gcacatcaca gtaaccgctg atcatagcaa accctctgaa ggcggaatat
    26881 cagcaaattc ctggagttat ttcaacctat tgacttttaa tttgatttga ggacgattat
    26941 ttattaagtt acataaatgt aaataacatg caattcacat tgccgaaagc gtgtccgata
    27001 tcgcacaata accgctgatt tcacagcata gatcccacga tgaacaaatc attacaaaaa
    27061 cccaccattc tgaatgttga aactgtagcc cgttcccgac tgtttaccgt cgagagtgtg
    27121 gatctggagt tcagcaatgg cgtgcggcgt gtttatgaac gaatgcgtcc aaccaaccgg
    27181 gaagcagtga tgattgtgcc gattgtggac gatcacctga tcctgatccg cgaatacgca
    27241 gtgggaactg aatcctacga attaggtttt tcgaaaggat taattgatcc gggtgaaagc
    27301 gtctacgaag ccgctaaccg cgagctaaaa gaagaggttg gatttggtgc gaacgatctg
    27361 acttttttga agaagctcag catggcaccg tcttactttt ccagtaaaat gaatatcgtg
    27421 gtcgcgcaag atctctaccc ggaatcgctg gaaggcgatg agccagagcc gctaccacag
    27481 gtgcgctggc cgctggcgca tatgatggat ttgctggaag accctgactt caacgaagcg
    27541 cgtaatgtca gcgcgctgtt cctcgtgcgc gaatggttga aagggcaggg gcgagtgtaa
    27601 atgcgtgaag tttgcctgat gcacaacgct tatcaggctt atggggttcc tgcaatatat
    27661 tgatttttca cgattttgtt agccggataa cgcgttcacc cgcatccggc ataaacaaat
    27721 gcactttgtc agccaactga aaaggcgccg aagcgccttt ttaatcagaa caattcctgt
    27781 gcctcaccat tatcgataat ggtcgttccc acctcgtgca ccgcctgttg tgtcggctgc
    27841 gtaccttcga tgaaatactc ttcgcggctg ttgccaccat tagctaactg cccggtgctg
    27901 cgatcgatat tcaccgtcac aatacccgga ggcggcgtca gcggctgctc cggcacacct
    27961 tcaagaacgg ctttcatata agcgtcccat gcaggctggg cactcttggc accgccttcg
    28021 taacctgaga tctgatcttt aatcgctccg gaagccgttg tatgaccgag attacgacgg
    28081 tgatcatcaa agccaatcca gaccgaggtc acaacgcccg gaccgtaacc cgagaaccac
    28141 gcatctttcg aactgttagt ggtaccggtt ttcccgccga tatcgcgacg ctgcaaatca
    28201 cgacctgcac gccagccggt accctgccag cctggttcac caaagatatt ggtgttcaaa
    28261 gcactcttaa tcaggaatgc cagcggagtg ttgatgacgt gcggtgcgta ctcctgcgcg
    28321 ccagtcttcg ccactaacgc ctgatttgcc tgctccagct gcggcattgg tacagaaaca
    28381 ttctgctgct cgcgggagat agcgacatct tcaacatcgt tattttccag cacgttcgat
    28441 ttctgcgtat caccgtaaat cactggaata tcgcattccg ggcaggctac tttcggtttc
    28501 gcttcgaaaa tcacgccgcc ctgatcgttt tcaattttgc tgataaacca cgggtccact
    28561 aggaagccgc cgttcgccat gaccgcgtag ccgcgcgcca cctgcattgg ggtgaaggac
    28621 gctgaaccca gcgccagcga ttcggtgtgg acaatgtttt gcgccgggaa gccgaagcgt
    28681 tgcagatatt ctgcagcgta gtcgacgccc atcgcccgca ttgcacgtac catcaccacg
    28741 tttttcgact gacccagccc ctgacgtaag cgaattggac cagcatactg cggtggtgag
    28801 ttcttcggct gccagtcaga accggcacct gcatcccagc gagaaattgg cacatcgttc
    28861 aacatacttg ccagcgtcag acctttatcc atcgccgcgg tgtagaggaa cggtttgatg
    28921 ttggaaccca cctgacgcag tgcctgggtg gcgcggttaa acttgctctg attgaaatca
    28981 aagccaccga ccagcgccat aacggcacca ttttgcggat tgatcgacac cagcgccgag
    29041 ttcacttccg gcacttgtgc cagccaccat gcatcgccaa cctgacgaac ccagatttgc
    29101 tgacccgttt gcagaacatc ggtcacttta cgcggcgtcg gtccctgctg agtatccgaa
    29161 cggtaaggac gcgcccagcg aacgccttcc atactcaatg cgacggtcga cccgtccgcc
    29221 agcatcgccg tcgctccctg aggattggcg ctggtgactg cggcaggcag cagcggacca
    29281 taggtcggca gcgccttcag cgtatcggta atcttgttgt tatcccacgc cgactcgccc
    29341 actttccaca gcacatttgc cgggccgcga tagccgtggc gcatgtcgta gtccagcacg
    29401 ttattacgta ccgcctgctg cgcggcctgc tgcactttgc gggtgatggt ggtgtaaatg
    29461 cgataaccgt cttcataggc actttcgcca taacggttat acatctcctg gcgcaccatt
    29521 tcgctcaggt acggcgcaga gaaagcaatc tccggcgcgt gataattagc gttaatcgcc
    29581 tcagtgcgtg tctgatcgaa ctgttgctgg gtgatatacc cttcatccag catccgcgac
    29641 agtacgacgt tacgccgcgc gacggcacga tccattgagt agagcggatt aaaggtggaa
    29701 ggcgctttcg gcaacccggc tatcaccgcc atttcgttca gcgtcagttg gtcgaccgtt
    29761 ttaccgaaat agacttgtgc cgcagcaccg acaccatagg cgcggtaacc aaggtaaatc
    29821 ttgttcagat aaagctcgag gatttcgtct ttcgtcagca actgttcaat gcgaatcgcg
    29881 aggaagactt ccttaatctt acgcatcagc gtgcgttctg gactgaggaa gaaatttctc
    29941 gccagctgct gggtaatggt actcgcccct tgtgacgcgt ggccggagaa cagcgccaca
    30001 cttgccgcac ggaagatccc caccgggtca acgccgtgat gctcgtagaa gcggctgtct
    30061 tccgtcgcga taaaggcttt caccatctcc ggtgggattt gatccaacgt aaccggaata
    30121 cgacgtttct caccgtattg agcaatcagc tcgccatcgg cgctgtaaat ctgcatcgga
    30181 atttgcaggc gaacatcttt taatgtcacc acatccggca gttgtggctc gatgtagcgg
    30241 tataggccat aaatcgagcc tgctcccagc agaatgcaac agactgcaag gatcaaaaaa
    30301 tactttacga acttcactgg aaatttccca tttagtttca tttgggcagt ttataaacaa
    30361 acgcgcggta gtataaaggc aagccagacg cattgatata cccgtcagag tgacgggtga
    30421 taaggagatc atcacaatga catttaagat ctggcaaatt ggtttgcatt tacaacagca
    30481 agaagtggca gcggttgcga tcgtgcgggg cgcaaaagaa tgctttttgc aacgctggtg
    30541 gcggttgccg ctggcgcacg acattatcaa agatgggcgg attgttgatg cgcagcagct
    30601 ggctaaaacg ttgttaccgt ggagtcgcga actgccgcag cgtcatcaca ttatgttggc
    30661 gtttcccgcc agtcgcacat tacagcggtc atttccgcgc ccgtcgatgt cccttggtga
    30721 gcgggagcaa acggcctggc tgtcagggac gatggcccgc gagctggata tggatccgga
    30781 ctccctgcgc ttcgattaca gcgaagactc actcagcccc gcttataacg tgactgccgc
    30841 gcaaagcaaa gagctggcaa cgctgcttac gctggcagaa aggttgcgtg ttcatgttag
    30901 tgcgatcacc ccggatgcca gtgcattaca gcgattcctg ccttttttac cttctcatca
    30961 gcaatgtctg gcctggcgtg ataacgaaca gtggctgtgg gcgacgcgct atagctgggg
    31021 gcgcaaactg gcggtgggga tgactagcgc gaaggagctg gcggcagcgt tatccgttga
    31081 tcccgacagc gtcgcgatat gtggcgaagg cggatttgac ccctgggagg ccgtttctgt
    31141 tcgtcagccg ccgctaccgc cgcctggtgg agactttgcc atcgcgctgg ggctggcgct
    31201 tgggaaggcg tactgatgaa cccgccaatt aattttttgc cctggcgaca gcaacgccgg
    31261 accgcttttc tgcgtttctg gttgctgatg ttcgttgcgc ctctgctgct ggccgtcggg
    31321 ataacgctaa tactgcgtct gacaggcagc gccgaagcgc gcatagacgc agttttgctt
    31381 caggcggaac aacaactcgc ccgcagctta cagataacga agccacgttt gctggagcaa
    31441 cagcagttac gcgaacagcg ttcgcaacgg cagcgccagc gacaatttac ccgcgactgg
    31501 caatctgcgc tggaagcact ggcggcgctt ttacccgagc acgcctggct gacaacgata
    31561 agctggcagc agggaacgct ggagatcaag gggctgacaa caagcattac cgcgttaaac
    31621 gcactagaaa cgtcactccg ccaggatgcg tcttttcatc tcaatcagcg gggagccacg
    31681 cagcaggatg cgcagggacg ctggcaattt gagtatcagt taacaaggaa ggttagcgat
    31741 gaacatgttc tttgactggt ggttcgccac atcaccccgc ctccgccagt tttgctgggc
    31801 attctggttg ctgatgttag ttacgctcat ttttctatca tcgacacacc atgaagagcg
    31861 cgacgcatta attcgactac gggcaagtca tcaccagcag tgggccgcac tgtatcgcct
    31921 ggtagacacc actcccttca gcgaggaaaa aacgctgccc ttttcgccac tggattttca
    31981 gttatccggc gcgcaactgg tttcctggca tccatccgcg cagggaggcg agttggcgtt
    32041 gaaaacgctg tgggaagcag tgccgtcggc atttacacgg ctggcagagc gcaacgtcag
    32101 cgtgagccgt ttttcgttaa gcgtggaagg tgatgatctt ttgttcacgc tacaactgga
    32161 gatgccgcat gagggttaaa cgctggttgt tggcaggtat tgcattgtgc cttttaaccg
    32221 gtatgcgtga cccttttaaa tcgccggaag atctatgccg gattagcgaa cttagccagt
    32281 ggcgctatca ggggatggta gggcgaggcg agcgcatcat cggtgtaata aaagacgggc
    32341 aaaagaaatg gcgacgggtg cagcaaaacg atgtgctgga aaacggctgg acaattttac
    32401 agctgacgcc agacgtacta acgctgggta ccgggacaaa ctgcgaaccg ccacaatggt
    32461 tgtggcaacg gcaaggagat acaaatgaag caatggatag ccgcactact gttgatgctg
    32521 atatccggcg tacaggcggc aaagccgcaa aaagtgacgc tgatggtgga tgacgttccg
    32581 gtagctcagg tgttgcaggc gctggctgaa caggagaagt tgaacctggt cgtgtcgcca
    32641 gacgtcagcg gtacggtgtc gttacatcta acagatgttc cctggaagca ggcactacaa
    32701 actgtagtga aaagcgccgg actgataacg cggcaggaag gcaacattct ctcagtgcat
    32761 tccattgcct ggcagaatga caatatcgcc cgccaggagg cggagcaggc gcgggcgcag
    32821 gcaaatctgc cgctggaaaa tcgcagtata accctgcaat acgccgacgc gggagaactg
    32881 gcgaaagcgg gggagaagct actgagtgcc aaagggagta tgaccgtcga taaacgcacc
    32941 aatcgccttt tgctacgaga taacaaaacg gcgttaagcg cgcttgaaca gtgggtagcg
    33001 caaatggatc tgccggtcgg gcaggttgag ctgtcggcgc atattgtcac cattaatgaa
    33061 aaaagtttgc gtgagttagg cgtgaaatgg acgctggccg atgcgcaaca cgctggtggc
    33121 gttgggcaag tcaccacgct tggtagcgac ctctccgtag cgacggcgac aacgcatgtc
    33181 ggttttaaca ttgggcgcat caacggacgc ttgctggatc ttgagctttc cgcgctcgaa
    33241 caaaaacagc agctggatat tatcgccagt ccgcgtctgc tggcctcaca tcttcagcct
    33301 gccagcatta aacaggggag cgaaattcca tatcaggttt ccagcgggga aagtggcgcg
    33361 acgtcggtgg aatttaaaga ggccgtcctg gggatggagg tcacgcccac ggtgttacaa
    33421 aaaggtcgca tccggctgaa attacacatc agccagaacg ttccggggca ggtgctacag
    33481 caggccgatg gcgaagtgct ggcgattgat aagcaggaga tcgaaacgca ggtcgaggta
    33541 aaaagcggag aaacgttggc gctgggcggc atttttaccc gtaaaaataa atcgggtcag
    33601 gatagcgtac cgttgcttgg cgacattccc tggttcgggc aattatttcg tcatgacgga
    33661 aaagaagatg aacgacgcga gttagtggtg tttatcacgc cacgactggt ttccagtgag
    33721 taaacagccg taaaagcggt aatgttttta tgctgaacgt gtttcatcta tttgacgcgc
    33781 gcaggtattt agcatacaag gagtaccgat ttgagagttg gtgctcttcg ctgcctgcgt
    33841 tccatgatga tgatttatca ttcaggcggc attttgctgt cttttttacg ctaatcttac
    33901 ccggtgattt atcgccagag cggtggtagc aaggcagtgc gcttgcagcg accagatatg
    33961 cagagggatg ggtgatttat tcagttgcca aacccgctgg agtattgaga taattttcag
    34021 tctgactctc gcaatatctt atgaggtttc agttcatgtc ctgcggcgct ctctgagcga
    34081 agcgggttta tcattaacga atagtcttag tagtaccgaa aaaatggcag agaaacgcaa
    34141 tatctttctg gttgggccta tgggtgccgg aaaaagcact attgggcgcc agttagctca
    34201 aaaactcaat atggaatttt acgattccga tcaagagatt gagaaacgaa ccggagctga
    34261 tgtgggctgg gttttcgatt tagaaggcga agaaggcttc cgcgatcgcg aagaaaaggt
    34321 catcaatgag ttgaccgaga aacagggtat tgtgctggct actggcggcg gctctgtgaa
    34381 atcccgtgaa acgcgtaacc gtctttccgc tcgtggcgtt gtcgtttatc ttgaaacgac
    34441 catcgaaaag caacttgcac gcacgcagcg tgataaaaaa cgcccgttgc tgcacgttga
    34501 aacaccgccg cgtgaagttc tggaagcgtt ggccaatgaa cgcaatccgc tgtatgaaga
    34561 gattgccgac gtgaccattc gtactgatga tcaaagcgct aaagtggttg caaaccagat
    34621 tattcacatg ctggaaagca actaattctg gctttatata cactcgtctg cgggtacagt
    34681 aattaaggtg gatgtcgcgt tatggagagg attgtcgtta ctctcgggga acgtagttac
    34741 ccaattacca tcgcatctgg tttgtttaat gaaccagctt cattcttacc gctgaaatcg
    34801 ggcgagcagg tcatgttggt caccagcgaa accctggctc ctctgtatct cgataaggtc
    34861 cgcggcgtac ttgaacaggc gggtgttaac gtcgatagcg ttatcctccc tgacggcgag
    34921 cagtataaaa gcctagctgt actcgatacc gtctttacgg cgttgttaca aaagccgcat
    34981 ggtcgcgata ctacgctggt ggcgcttggc ggcggcgtag tgggcgatct gaccggcttc
    35041 gcggcggcga gttatcagcg cggtgttcgt ttcattcaag tcccgacgac gttactgtcg
    35101 caggtcgatt cctccgttgg cggcaaaact gcggtcaacc atcccctcgg taaaaacatg
    35161 attggcgcgt tctaccagcc tgcttcagtg gtggtggatc tcgactgtct gaaaacgctt
    35221 cccccgcgtg agttagcgtc ggggctggca gaagtcatca aatacggcat tattcttgac
    35281 ggtgcgtttt ttaactggct ggaagagaat ctggatgcgt tgttgcgtct ggacggtccg
    35341 gcaatggcgt actgtattcg ccgttgttgt gaactgaagg cagaagttgt cgctgccgac
    35401 gagcgcgaaa ccgggttacg tgctttactg aatctgggac acacctttgg tcatgccatt
    35461 gaagctgaaa tggggtatgg caattggtta catggtgaag cggtcgctgc gggtatggtg
    35521 atggcggcgc ggacgtcgga acgtctcggg cagtttagtt ctgccgaaac gcagcgtatt
    35581 ataaccctgc tcacgcgggc tgggttaccg gtcaatgggc cgcgcgaaat gtccgcgcag
    35641 gcgtatttac cgcatatgct gcgtgacaag aaagtccttg cgggagagat gcgcttaatt
    35701 cttccgttgg caattggtaa gagtgaagtt cgcagcggcg tttcgcacga gcttgttctt
    35761 aacgccattg ccgattgtca atcagcgtaa caacaagaaa ggtcaggccg cttatcaagc
    35821 ggtctattag cttcaggtta attgcaacgt ggtaagcatt aaccttttag tggggtgtta
    35881 aatggatgaa ttcaaaccag aagacgagct gaaacccgat cccagcgatc gtcgtactgg
    35941 tcgttctcgt caatcttctg aacgttctga gcgtactgaa cgtggcgaac cgcagatcaa
    36001 ttttgatgat attgaacttg atgacactga cgatcgccgt ccgactcgtg cgcaaaaaga
    36061 gcgcaatgag gaaccggaaa tcgaagaaga aattgacgaa tccgaagatg aaaccgtgga
    36121 tgaagagcgc gtagagcgtc gtccgcgtaa gcgcaaaaaa gcagccagta aacccgcttc
    36181 tcgtcagtat atgatgatgg gcgtcggcat tctggttcta ctgctgttga tcatcggtat
    36241 cggttctgcg ctaaaagccc cctcgaccac ttccagcgat caaaccgcgt ctggcgagaa
    36301 gagtattgat cttgctggca atgcgaccga tcaggcgaat ggcgtgcagc cagcgccggg
    36361 aaccacgtct gcggaaaata ctcagcagga tgtttctctg ccaccgatct cttctacgcc
    36421 gactcaaggg caaaccccgg tggcaacgga tggtcaacaa cgtgttgaag tgcagggtga
    36481 cctgaacaat gcgctgaccc agccacaaaa tcagcaacag ctgaacaatg tggcggtcaa
    36541 ttccacgttg ccgactgaac ccgcaacggt tgcgcctgtt cgcaatggca atgcatcgcg
    36601 tgacacggcg aaaacgcaaa ccgctgaacg tccgtccact acgcgcccag ttcgtcagca
    36661 ggcggtgatt gaaccgaaaa aaccgcaagc aaccgtgaaa acggagccga agccggtagc
    36721 acagacgccg aagcgtactg aaccagctgc ccctgtggcg agcacgaagg caccggctgc
    36781 gacttctacg ccagcaccaa aagagacggc gactacggct ccagtacaga cggcatcccc
    36841 ggcgcaaacc acggcaacac cagccgctgg agggaagacc gcaggtaatg ttggttcgtt
    36901 gaaatcggca ccgtccagcc attacactct gcagctgagc agttcctcta actacgacaa
    36961 cctgaacggt tgggcgaaga aagagaatct gaaaaactac gttgtctatg aaacgacgcg
    37021 taatggtcag ccgtggtatg tcctggtttc tggcgtgtac gcttcgaaag aagaggcgaa
    37081 aaaagcggta tctacattgc cagcagatgt tcaggccaaa aacccgtggg cgaaaccgct
    37141 gcgtcaggta caggccgatc tgaagtaatc aaggttatct cccgcaatgg tttatcgttg
    37201 cgggagctgc ctgaagcgct ggatgctgtc ggagctttct ccacagccgg agaaggtgta
    37261 attagttagt cggcatgaag aaaaatcgcg cttttttgaa gtgggcaggg ggcaagtatc
    37321 ccctgcttga tgatattaaa cggcatttgc ccaagggcga atgtctggtt gagccttttg
    37381 taggtgccgg gtcggtgttt ctcaacaccg acttttctcg ttatatcctt gccgatatca
    37441 atagcgacct gatcagtctc tataacattg tgaagatgcg tactgatgag tacgtacagg
    37501 ccgcacgcga gctgtttgtt cccgaaacaa attgcgccga ggtttactat cagttccgcg
    37561 aagagttcaa caaaagccag gatccgttcc gtcgggcggt actgttttta tatttgaacc
    37621 gctacggtta caacggcctg tgtcgttaca atctgcgcgg tgagtttaac gtgccgtttg
    37681 gccgctacaa aaaaccctat ttcccggaag cagagttgta tcacttcgct gaaaaagcgc
    37741 agaatgcctt tttctattgt gagtcttacg ccgatagcat ggagcgcgca gatgatgcat
    37801 ccgtcgtcta ttgcgatccg ccttatgcac cgctgtctgc gaccgccaac tttacggcgt
    37861 atcacacaaa cagttttacg cttgaacaac aagcgcatct ggcggagatc gccgaaggtc
    37921 tggttgagcg ccatattcca gtgctgatct ccaatcacga tacgatgtta acgcgtgagt
    37981 ggtatcagcg cgcaaaattg catgtcgtca aagttcgacg cagtataagc agcaacggcg
    38041 gcacacgtaa aaaggtggac gaactgctgg ctttgtacaa accaggagtc gtttcacccg
    38101 cgaaaaaata attctcaagg agaagcggat gaaacagtat ttgattgccc cctcaattct
    38161 gtcggctgat tttgcccgcc tgggtgaaga taccgcaaaa gccctggcag ctggcgctga
    38221 tgtcgtgcat tttgacgtca tggataacca ctatgttccc aatctgacga ttgggccaat
    38281 ggtgctgaaa tccttgcgta actacggcat taccgcccct atcgacgtac atctgatggt
    38341 gaaacccgtc gatcgcattg tgcctgattt cgctgccgct ggtgccagca tcattacctt
    38401 tcatccagaa gcctccgagc atgttgaccg cacgctgcaa ctgattaaag aaaatggctg
    38461 taaagcgggt ctggtattta acccggcgac acctctgagc tatctggatt acgtgatgga
    38521 taagctggat gtgatcctgc tgatgtccgt caaccctggt ttcggcggtc agtctttcat
    38581 tcctcaaaca ctggataaac tgcgcgaagt acgtcgccgt atcgacgagt ctggctttga
    38641 cattcgtctg gaagtggacg gtggcgtgaa ggtgaacaac attggcgaaa tcgctgcggc
    38701 gggcgcggat atgttcgtcg ccggttcggc aatcttcgac cagccagact ataaaaaagt
    38761 cattgatgaa atgcgcagtg aactggcaaa ggtaagtcat gaataagttt gaagatattc
    38821 gcggcgtcgc ttttgatctt gatggtacgc tggtcgacag tgctcctggt cttgctgctg
    38881 cggtagatat ggcgctgtat gcgctggagt tgcccgtcgc aggtgaagaa cgcgttatta
    38941 cctggattgg taacggcgca gatgttctga tggagcgcgc attgacctgg gcgcgtcagg
    39001 aacgtgcgac tctgcgtaaa acaatgggga aaccgcccgt tgatgacgac attccggcag
    39061 aagaacaggt acgtattctg cgtaaactgt tcgatcgcta ctatggcgag gttgccgaag
    39121 aggggacgtt tttgttcccg cacgttgccg atacgttggg cgcgttgcag gctaaaggcc
    39181 tgccgctagg cctggtcacc aacaaaccga cgccgttcgt cgcgccgctg ctcgaagcct
    39241 tagatattgc caaatacttt agcgtggtta tcggcggcga tgatgtgcaa aacaaaaaac
    39301 cgcatccgga cccgctgtta ctggtggctg agcggatggg aattgcccca caacagatgc
    39361 tgtttgtcgg cgactcacgc aatgatattc aggcggcaaa agcggcaggt tgcccatcag
    39421 ttggcttaac ctacggatat aactacggcg aggctatcga tctcagccag cctgatgtaa
    39481 tttatcagtc tataaatgac cttctgcccg cattagggct tccgcatagc gaaaatcagg
    39541 aatcgaaaaa tgactaagcc catcgttttt agtggcgcac agccctcagg tgaattgacc
    39601 attggtaact acatgggtgc gctgcgtcag tgggtaaaca tgcaggatga ctaccattgc
    39661 atttactgta tcgttgacca acacgcgatc accgtgcgcc aggatgcaca gaagctgcgt
    39721 aaagcgacgt tggatacgct ggccttgtat ctggcttgtg gtatcgatcc tgagaaaagc
    39781 accatttttg ttcagtccca cgtaccagaa catgcgcagt taggctgggc actgaactgc
    39841 tatacctact tcggcgaact gagtcgcatg acgcagttta aagataaatc tgcgcgttat
    39901 gccgagaaca tcaacgctgg tctgtttgac tatccggtgc tgatggctgc ggacatcctg
    39961 ctgtatcaaa ctaatctggt accggtgggt gaagaccaga aacagcacct ggaactgagt
    40021 cgcgatatcg cccagcgttt caacgcgctg tatggcgaga tctttaaggt gccggagccg
    40081 tttattccga aatctggcgc gcgcgtaatg tcgctgctgg agccgaccaa gaagatgtcc
    40141 aagtctgacg ataaccgcaa taacgttatc ggcctgctgg aagatccgaa atcggtagtg
    40201 aagaaaatca aacgcgcggt cactgactcc gacgagccgc cggtagttcg ctacgatgtg
    40261 cagaacaaag cgggcgtttc caacctgctg gatatccttt ctgcggtaac gggccagagc
    40321 atcccagaac tggaaaaaca gttcgaaggc aagatgtatg gtcatctgaa aggtgaagtg
    40381 gctgatgccg tttccggtat gctgactgaa ttgcaggaac gctatcaccg tttccgcaac
    40441 gatgaagcct tcctgcaaca ggtgatgaaa gacggcgcag aaaaagccag tgtacacgct
    40501 tcccgtacgc tgaaagcggt gtacgaagcg attggttttg tggcgaagcc gtaatatcaa
    40561 aacgctcgga cggtcccctc gcccctttgg ggagagggtt agggtgaggg gagcaggcaa
    40621 gcgccagtgg gaattaccct ttgctcgcat tgtcgaataa atcacttcgt catcactctg
    40681 aaaccgggga aacccggttt ttttatcctc ttttcgcaac aacccgaaaa aatgtgaagc
    40741 gcctcgccgt ttccacatct cgtcgtcgcc acttatcttc attttaatga agatataaat
    40801 attcatttta ttgaaaatat attatgcgtc gaacgtttat caaaaaagaa ggcgtcgtca
    40861 tcacgacact ggcccgttat ttgttgggtg aaaaatgcgg taatcgactg aaaaccatag
    40921 atgagctggc aactgaatgc cgttcatcgg ttggcctgac gcaggccgcg ttgaaaacgc
    40981 tggaatcaag cggagcgata cggattgaac gccgtgggcg caatggcagt tatcttgtcg
    41041 agatggataa caaagcattg ctgagtcatg tggatatcaa caacgtggta tgtgcgatgc
    41101 ctttgcccta tacccgcttg tacgaaggtt tagcgagcgg attgaaagcc cagtttgatg
    41161 gcattccttt ttactatgcg catatgcgcg gcgcggatat tcgcgtggag tgtttactta
    41221 acggtgtcta tgacatggca gtagtttccc gcctggcggc ggaaagttat ctcagccaaa
    41281 acaatttgtg catcgccctc gagcttggcc cgcatacgta tgttggtgaa catcaactga
    41341 tatgccgcaa aggtgagtct ggaaatgtta agcgagtagg tctcgatagt cgttcggcgg
    41401 atcagaaaat catgaccgat gtcttttttg gcgatagtga tgtggaacga gtcgatctct
    41461 cttatcacga gagtttacaa cgcattgtta aaggcgatgt cgatgcggtg atctggaacg
    41521 tggtggcgga aaacgaactg accatgctgg gattagaggc gacgccgcta acagacgatc
    41581 cgcgattttt actggccacc gaagccgtgg tcctgacgcg agtcgatgat tacccaatgc
    41641 aacaattgct gcgtgccgtt gtagataaac acgccctgct tgcccatcaa caacgggtgg
    41701 tgagtgggga acaggaaccc agttattaaa cgaaaggcat ctgatatgga aaccagactc
    41761 aacctgcttt gcgaggcagg cgttattgat aaggacgtct gcaaaggcat gatgcaggtc
    41821 gtcaacgtac tggaaaaaga gtgccatctg ccggtgcgca gtgagcaagg aacgatggcg
    41881 atgacacata tggcgagtgc actgatgcgc agtcgccgtg gtgaagaaat agagccgctg
    41941 gataacgagt tgctggcgga actggcgcaa tccagccact ggcaagccgt tgtgcaattg
    42001 catcaggtgt tgttgaagga attcgctctg gaagttaacc cgtgtgaaga aggctatttt
    42061 ctggccaatc tttatggatt atggatggct gctaacgaag aggtttgaag ctcccgacaa
    42121 atccacgtcg cacaacctaa atatttaaaa aaatgaatat ttaattcaag gaacagaaac
    42181 gaggcaacaa gatgtttgta gaagcattga aacgccagaa cccggcgctg atttccgccg
    42241 cactaagttt gtggcagcag ggcaaaatcg ccccggacag ctgggtgatc gacgtggatc
    42301 agatactgga aaacggtaag cggctcattg agacggcgcg gctttacggc attgaactgt
    42361 atctgatgac caagcaattt ggtcgtaatc cgtggctggc ggaaaaattg ctggcattag
    42421 gctacagcgg cattgtggcg gtggattaca aagaggcgcg agtcatgcgc cgcgctggtt
    42481 tgccagtggc gcatcagggg catctggtac aaatcccttg tcatcaggtt gctgacgccg
    42541 ttgaacaggg caccgacatc atcaccgtgt ttactctcga caaagcacgg gaagtttctg
    42601 cggcagcggt taaggccgga cgggtccagc ctgtgctgct caaagtttat agcgacgatg
    42661 attttcttta tccggggcag gagagcggtt ttgtacagca ttcgctgcac gaggtgattg
    42721 ctgaaattaa gaaactgccg gggctgcatt tagccggact aacccatttc ccttgcctgc
    42781 tttgggatga ggctgccggg aaagttttgc caacaccgaa tcttcacacg ctggtacaag
    42841 cacgggatca actggcgaaa tctggtattg cgattgagca actgaacgta ccttcagcga
    42901 ccagctgcac ttcgctgcca ttgctggcgg aatacggtgc gactcatgcc gaacccggtc
    42961 atgcgctgac cggtactatt ccggcaaacc agcagggcga tcagcctgaa cgtatcgcga
    43021 tgctctggtt aagtgaaatc tcccatcatt tccgtggcga cagctactgc tacggcggcg
    43081 gttactaccg tcgtggtcat gcgcaacatg cgctggtgtt tacgccagaa aatcaaaaga
    43141 ttactgaaac caatctcaaa accgtggatg acagcagtat cgactatacc ctgccgctgg
    43201 caggcgagta tccggtgagc agtgcagtgg tgctctgttt tcgcacgcag atttttgtca
    43261 cccgtagcga tgtggtgctg gtgtccggta ttcagcgcgg cgaaccagaa atagttggtc
    43321 gttatgacag cctcggcaac cctctgaagg cataaatggc gcgatttgtg gtgttagtga
    43381 ttgatagctt tggcgtaggg gcaatgaaag atgtcacgct ggtgcgtccg caagatgccg
    43441 gggcgaatac ctgtggtcac atcctgagcc agttgccgca tttgcagcta ccaacgttgg
    43501 agaagctggg gctaatcaac acattgggtt atgcgccagg cgatatgcag ccgtcagatt
    43561 ccgcaacctg gggcgtggca gagctgcaac atgaaggtgg cgataccttt atggggcatc
    43621 aggaaatttt aggcacgcgc ccgttaccgc cgctgcggat gccttttcgc gatgtgattg
    43681 accgtgttga gcaggcatta gtttccgctg gctggcaggt ggagcgccgt ggcgatgatc
    43741 tgcaatttct gtgggtcaat caggcggttg cgattggcga taatctcgag gcggatttag
    43801 gccaagtcta taacattacc gccaatctct ctgtgatctc ttttgacgac gcaatcaaaa
    43861 ttggtcgtat cgtgcgtgac caggtacagg tcggtcgggt cattacattt ggcggcctgt
    43921 taaccaacag tcagcgtatt ctcgatgccg cagaaagcaa agaagggcgc tttattggta
    43981 ttaatgcgcc gcgttctggc gcttatgaca acggcttcca ggtcgtgcat atgggctacg
    44041 gtgttgatga aaaagtgcag gtgccgcaaa aactgtatga agcgggcgtg ccaaccgtgc
    44101 tggtgggtaa ggtggcagat atcgtcagca atccttatgg tgtgagctgg caaaacctgg
    44161 tggatagcca gcggattatg gatatcaccc tcgacgaatt taacacccat ccgacggcgt
    44221 ttatttgcat caatatccag gaaaccgacc tcgctggtca tgcagaagac gtcgcacgtt
    44281 atgccgaacg tttgcaggtc gttgaccgta accttgcccg gcttgttgag gcgatgcagc
    44341 cagatgattg cctggtcgtg atggcggatc acggtaacga tccgaccatt ggtcacagcc
    44401 accatacccg tgaagtggtg ccagtgctgg tttatcagca agggctggtc gccacgcagc
    44461 tcggtgtgcg caccacgctt tctgatgtgg gagctaccgt gtgtgaattt ttccgtgcgc
    44521 caccgccaca aaatggtcgc tcttttcttt cctccctccg gtttgcagga gacaccctat
    44581 gagttttgat ccgacgggtt acaccctggc ccatgagcat ctgcatattg atctctccgg
    44641 ctttaaaaac aacgtggact gccgccttga tcagtatgcg ttcatttgcc aggagatgaa
    44701 cgacctgatg acccggggtg tacgtaatgt gattgagatg accaaccgtt acatggggcg
    44761 caatgcgcaa tttatgctcg atgtaatgcg cgaaaccggg atcaacgtgg tggcctgtac
    44821 tggttattac caggatgcgt ttttcccgga acatgtggtg acccgcagcg tgcaggaact
    44881 ggcgcaggag atggtcgatg aaattgaaca gggtatcgat ggcacggagc tgaaagccgg
    44941 gatcatcgcg gagatcggct ccagcgaagg aaagattacg ccgctggaag agaaggtatt
    45001 tattgccgct gcgctggcgc ataaccagac cggacgcccg atctccacgc atacgtcgtt
    45061 cagcaccatg gggctggaac aactggctct gttgcaagcc cacggagttg atctttcgcg
    45121 cgtcaccgtt ggtcactgcg atctgaaaga caacctcgac aacattttga agatgatcga
    45181 tctcggcgcg tacgtgcagt tcgacaccat tggtaagaac agttactacc cggacgaaaa
    45241 gcgcattgcg atgctccacg cgctacgtga ccgtgggttg ctgaaccgcg tcatgctgtc
    45301 gatggatatt acgcgccgct cccatttaaa agccaatggt ggttatggct atgactattt
    45361 attgaccacc tttattccgc aattgtgcca gtcaggattc agtcaggccg atgtggatgt
    45421 gatgttacgt gaaaatccct ctcaattttt ccaataagga cagactcatg aaaaagattg
    45481 gcgttgcagg cttacagcgt gagcagatta aaaaaaccat tgaagcgacg gctcctggct
    45541 gttttgaagt tttcattcac aacgacatgg aagcggcaat gaaggtgaaa tccgggcaac
    45601 tagattatta cattggcgcg tgtaataccg gtgcgggcgc ggcataaagc gaaagacgag
    45661 catatcgcca aaatgatcgc tgaagggaaa gtggcgtttg gcctttccgt tgagcacgtc
    45721 gaacacgcga ttccgatgtt gattaaccat ctgaaataaa aggcacgact atggatctgt
    45781 atattcagat tatcgtggtg gcgtgcctga cgggtatgac atcgcttctg gcgcatcgct
    45841 cggcggctgt ttttcatgac ggcatccacc cgatcctgcc gcaactgatt gaaggctata
    45901 tgaaccgtcg cgaggcgggg agtatcgctt ttggtctgag cattggtttt gtggcctcgg
    45961 tggggatctc ttttaccctg aaaacctggc tgctcaacgc atggttactc tttcttccta
    46021 ccgatatcct cggcgtactg gcgataaaca gcctgatggc gtttggtctt ggcgctatct
    46081 ggggcgtgtt gatccttact tgcctgttgc cagttaacca gctgctgacc gcgctgccgg
    46141 tggatgtatt aggtagcctc ggggaattaa gctcgccggt ggtttctgct tttgcactgt
    46201 tcccgctggt ggcgattttc taccagtttg gctggaagca aagtctggtc gccgccgtgg
    46261 ttgttctgat gacccgtgtg gtagtcgtgc gctatttccc acatcttaac cctgaatcca
    46321 tcgaaatctt tattggcatg gtgatgctgc tggggatcgc gataactcac gacctgcgtc
    46381 atcgtgatga aaatgacatc gatgccagcg ggctttcggt gtttgaagag cgcacgtcgc
    46441 ggattatcaa aaacttacct tatatcgcca tcgtgggagc attgattgcc gccgttgcca
    46501 gtatgaagat ttttgctggt agcgaagttt cgatcttcac actggagaaa gcgtattccg
    46561 caggcgtgac gccggaacaa tcgcaaacgc tgattaatca ggcggctctg gcagaattta
    46621 tgcgcggact ggggtttgtg ccgttgattg ccaccaccgc cttagcaacg ggtgtgtatg
    46681 cagttgcggg ctttaccttt gtttatgcgg tgggctatct ctcgccgaat ccgatggttg
    46741 cagcggtatt aggcgcagtg gttatttcgg cggaagtctt gctgcttcgt tcgatcggca
    46801 aatggctggg acgctacccg tcggtgcgta atgcgtcgga taacatccgt aacgccatga
    46861 atatgctgat ggaaatggcg ctgctggtcg gttcgatttt cgcagcaatt aagatggcgg
    46921 gttataccgg attctctatc gcggttgcta tttacttcct caacgaatcc ctgggccgtc
    46981 cggtacagaa aatggcggca ccggtcgtgg cagtaatgat caccggtatt ctgctgaatg
    47041 ttctttactg gcttggcctg ttcgttccgg cttaaggaag gctcctatga agacgtttcc
    47101 tttgcaaagc ctgactcttg ctgaagcgca gcaaaagcag tttgcgctgg tggacactat
    47161 ttgccgccat tttccaggca gcgagtttct tgctggcggt gatttagggt taactccagg
    47221 tctgaatcaa ccgcgtatta cccaacgggt ggagcaggtg ctggctgatg c
//
LOCUS       DAJTYI010000015        43624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYI010000015 DAJTYI010000000
VERSION     DAJTYI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..562
                     /locus_tag="OO564_000849"
                     /pseudo
     CDS             1..562
                     /locus_tag="OO564_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            632..1408
                     /gene="yaaA"
                     /locus_tag="OO564_000850"
     CDS             632..1408
                     /gene="yaaA"
                     /locus_tag="OO564_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6652080.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(1561..1857)
                     /locus_tag="OO564_000851"
     CDS             complement(1561..1857)
                     /locus_tag="OO564_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6652081.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2071..3357)
                     /gene="thrC"
                     /locus_tag="OO564_000852"
     CDS             complement(2071..3357)
                     /gene="thrC"
                     /locus_tag="OO564_000852"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6652082.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(3358..4290)
                     /gene="thrB"
                     /locus_tag="OO564_000853"
     CDS             complement(3358..4290)
                     /gene="thrB"
                     /locus_tag="OO564_000853"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6652083.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4292..6754)
                     /gene="thrA"
                     /locus_tag="OO564_000854"
     CDS             complement(4292..6754)
                     /gene="thrA"
                     /locus_tag="OO564_000854"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6652084.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6780..6899)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6835..6900)
                     /gene="thrL"
                     /locus_tag="OO564_000855"
     CDS             complement(6835..6900)
                     /gene="thrL"
                     /locus_tag="OO564_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6652085.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7114..7800)
                     /locus_tag="OO564_000856"
     CDS             complement(7114..7800)
                     /locus_tag="OO564_000856"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6652086.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8200..8340)
                     /locus_tag="OO564_000857"
     CDS             complement(8200..8340)
                     /locus_tag="OO564_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652087.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8436..9152
                     /gene="arcA"
                     /locus_tag="OO564_000858"
     CDS             8436..9152
                     /gene="arcA"
                     /locus_tag="OO564_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6652088.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9211..10563)
                     /gene="creD"
                     /locus_tag="OO564_000859"
     CDS             complement(9211..10563)
                     /gene="creD"
                     /locus_tag="OO564_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6652089.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10621..12045)
                     /gene="creC"
                     /locus_tag="OO564_000860"
     CDS             complement(10621..12045)
                     /gene="creC"
                     /locus_tag="OO564_000860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6652090.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(12045..12734)
                     /gene="creB"
                     /locus_tag="OO564_000861"
     CDS             complement(12045..12734)
                     /gene="creB"
                     /locus_tag="OO564_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6652091.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12747..13220)
                     /gene="creA"
                     /locus_tag="OO564_000862"
     CDS             complement(12747..13220)
                     /gene="creA"
                     /locus_tag="OO564_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6652092.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13431..14300
                     /gene="robA"
                     /locus_tag="OO564_000863"
     CDS             13431..14300
                     /gene="robA"
                     /locus_tag="OO564_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6652093.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14297..14944)
                     /gene="gpmB"
                     /locus_tag="OO564_000864"
     CDS             complement(14297..14944)
                     /gene="gpmB"
                     /locus_tag="OO564_000864"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6652094.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14996..15517
                     /gene="yjjX"
                     /locus_tag="OO564_000865"
     CDS             14996..15517
                     /gene="yjjX"
                     /locus_tag="OO564_000865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6652095.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15602..15928)
                     /gene="trpR"
                     /locus_tag="OO564_000866"
     CDS             complement(15602..15928)
                     /gene="trpR"
                     /locus_tag="OO564_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6652096.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16018..17955)
                     /gene="sltY"
                     /locus_tag="OO564_000867"
     CDS             complement(16018..17955)
                     /gene="sltY"
                     /locus_tag="OO564_000867"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6652097.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18166..19833
                     /gene="ettA"
                     /locus_tag="OO564_000868"
     CDS             18166..19833
                     /gene="ettA"
                     /locus_tag="OO564_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6652098.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19940..20107)
                     /locus_tag="OO564_000869"
     CDS             complement(19940..20107)
                     /locus_tag="OO564_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652099.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(20141..21373)
                     /gene="nadR"
                     /locus_tag="OO564_000870"
     CDS             complement(20141..21373)
                     /gene="nadR"
                     /locus_tag="OO564_000870"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6652100.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(21394..22776)
                     /gene="radA"
                     /locus_tag="OO564_000871"
     CDS             complement(21394..22776)
                     /gene="radA"
                     /locus_tag="OO564_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6652101.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22825..23793)
                     /gene="serB"
                     /locus_tag="OO564_000872"
     CDS             complement(22825..23793)
                     /gene="serB"
                     /locus_tag="OO564_000872"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6652102.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23899..24543
                     /locus_tag="OO564_000873"
     CDS             23899..24543
                     /locus_tag="OO564_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6652103.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24571..25587
                     /gene="lplA"
                     /locus_tag="OO564_000874"
                     /pseudo
     CDS             24571..25587
                     /gene="lplA"
                     /locus_tag="OO564_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            25619..25882
                     /locus_tag="OO564_000875"
     CDS             25619..25882
                     /locus_tag="OO564_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6652104.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(26043..26762)
                     /gene="deoD"
                     /locus_tag="OO564_000876"
     CDS             complement(26043..26762)
                     /gene="deoD"
                     /locus_tag="OO564_000876"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6652105.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26819..28042)
                     /gene="deoB"
                     /locus_tag="OO564_000877"
     CDS             complement(26819..28042)
                     /gene="deoB"
                     /locus_tag="OO564_000877"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6652106.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28094..29416)
                     /gene="deoA"
                     /locus_tag="OO564_000878"
     CDS             complement(28094..29416)
                     /gene="deoA"
                     /locus_tag="OO564_000878"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6652107.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(29543..30322)
                     /gene="deoC"
                     /locus_tag="OO564_000879"
     CDS             complement(29543..30322)
                     /gene="deoC"
                     /locus_tag="OO564_000879"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6652108.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30580..32121
                     /locus_tag="OO564_000880"
     CDS             30580..32121
                     /locus_tag="OO564_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6652109.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPHVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            32093..32956
                     /locus_tag="OO564_000881"
     CDS             32093..32956
                     /locus_tag="OO564_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6652110.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33170..33952)
                     /locus_tag="OO564_000882"
     CDS             complement(33170..33952)
                     /locus_tag="OO564_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6652111.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(33949..35022)
                     /locus_tag="OO564_000883"
     CDS             complement(33949..35022)
                     /locus_tag="OO564_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6652112.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(35144..35305)
                     /locus_tag="OO564_000884"
     CDS             complement(35144..35305)
                     /locus_tag="OO564_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6652113.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(35432..36037)
                     /gene="osmY"
                     /locus_tag="OO564_000885"
     CDS             complement(35432..36037)
                     /gene="osmY"
                     /locus_tag="OO564_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6652114.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(36430..38019)
                     /gene="prfC"
                     /locus_tag="OO564_000886"
     CDS             complement(36430..38019)
                     /gene="prfC"
                     /locus_tag="OO564_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6652115.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(38110..38787)
                     /gene="yjjG"
                     /locus_tag="OO564_000887"
     CDS             complement(38110..38787)
                     /gene="yjjG"
                     /locus_tag="OO564_000887"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6652116.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(38802..39248)
                     /gene="rimI"
                     /locus_tag="OO564_000888"
     CDS             complement(38802..39248)
                     /gene="rimI"
                     /locus_tag="OO564_000888"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6652117.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(39217..39630)
                     /gene="holD"
                     /locus_tag="OO564_000889"
     CDS             complement(39217..39630)
                     /gene="holD"
                     /locus_tag="OO564_000889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6652118.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            39733..40764
                     /gene="rsmC"
                     /locus_tag="OO564_000890"
     CDS             39733..40764
                     /gene="rsmC"
                     /locus_tag="OO564_000890"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6652119.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            41041..41127
                     /locus_tag="OO564_000891"
     tRNA            41041..41127
                     /locus_tag="OO564_000891"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41075..41077,aa:Leu,seq:cag)
     gene            41156..41242
                     /locus_tag="OO564_000892"
     tRNA            41156..41242
                     /locus_tag="OO564_000892"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41190..41192,aa:Leu,seq:cag)
     gene            41277..41363
                     /locus_tag="OO564_000893"
     tRNA            41277..41363
                     /locus_tag="OO564_000893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41311..41313,aa:Leu,seq:cag)
     gene            complement(41402..41638)
                     /locus_tag="OO564_000894"
     CDS             complement(41402..41638)
                     /locus_tag="OO564_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6652120.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            41779..42567
                     /gene="fhuF"
                     /locus_tag="OO564_000895"
     CDS             41779..42567
                     /gene="fhuF"
                     /locus_tag="OO564_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6652121.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(42605..43282)
                     /gene="bglJ"
                     /locus_tag="OO564_000896"
     CDS             complement(42605..43282)
                     /gene="bglJ"
                     /locus_tag="OO564_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6652122.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(43240..>43624)
                     /locus_tag="OO564_000897"
     CDS             complement(43240..>43624)
                     /locus_tag="OO564_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6652123.1"
                     /translation="IENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSE
                     RKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARE
                     LNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        10385 a        11921 c        10961 g        10357 t
ORIGIN      
        1 gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca ccctggtcat
       61 ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct
      121 ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct agggtgctga
      181 atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta
      241 tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg
      301 gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc tgatgtggca
      361 actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
      421 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
      481 cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc cggacgcgtg
      541 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
      601 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
      661 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
      721 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
      781 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
      841 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
      901 cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
      961 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     1021 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     1081 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     1141 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     1201 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     1261 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     1321 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     1381 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     1441 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     1501 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     1561 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     1621 gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     1681 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     1741 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     1801 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     1861 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     1921 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     1981 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     2041 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     2101 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     2161 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     2221 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     2281 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     2341 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     2401 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     2461 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     2521 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     2581 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     2641 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     2701 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     2761 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     2821 cgtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     2881 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     2941 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     3001 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     3061 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     3121 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     3181 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     3241 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     3301 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     3361 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     3421 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     3481 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc
     3541 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     3601 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     3661 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     3721 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     3781 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     3841 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     3901 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     3961 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     4021 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     4081 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     4141 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     4201 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     4261 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     4321 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     4381 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     4441 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     4501 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     4561 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     4621 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     4681 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     4741 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     4801 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     4861 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     4921 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     4981 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga
     5041 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     5101 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     5161 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     5221 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     5281 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     5341 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     5401 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     5461 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     5521 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     5581 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     5641 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     5701 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     5761 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     5821 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     5881 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     5941 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     6001 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     6061 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     6121 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     6181 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     6241 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     6301 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     6361 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     6421 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     6481 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     6541 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     6601 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     6661 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     6721 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     6781 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     6841 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     6901 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     6961 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     7021 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     7081 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     7141 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
     7201 taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac
     7261 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
     7321 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
     7381 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
     7441 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
     7501 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
     7561 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
     7621 aactttaata ttatcaataa tatcactaga tccatgtgcg acccagcggg tggctggctc
     7681 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
     7741 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
     7801 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
     7861 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
     7921 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
     7981 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
     8041 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
     8101 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
     8161 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
     8221 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
     8281 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
     8341 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
     8401 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
     8461 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
     8521 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
     8581 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
     8641 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
     8701 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
     8761 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
     8821 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
     8881 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
     8941 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
     9001 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
     9061 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
     9121 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
     9181 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
     9241 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg
     9301 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
     9361 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgagggt
     9421 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
     9481 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
     9541 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
     9601 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
     9661 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
     9721 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
     9781 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
     9841 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
     9901 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
     9961 cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta aagggatatg
    10021 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    10081 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    10141 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    10201 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    10261 ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact
    10321 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    10381 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    10441 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    10501 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    10561 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    10621 ctatgtgaag ttacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    10681 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    10741 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    10801 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    10861 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg tgttatccag
    10921 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    10981 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    11041 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    11101 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    11161 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    11221 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    11281 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    11341 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    11401 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    11461 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    11521 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    11581 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    11641 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    11701 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    11761 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    11821 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    11881 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    11941 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    12001 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    12061 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    12121 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    12181 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctagcga cttgagtaac
    12241 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    12301 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    12361 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    12421 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    12481 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    12541 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    12601 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    12661 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    12721 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    12781 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    12841 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    12901 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    12961 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    13021 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    13081 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    13141 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    13201 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    13261 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaggcc
    13321 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    13381 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    13441 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    13501 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    13561 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    13621 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    13681 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    13741 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtaaattca
    13801 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    13861 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    13921 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    13981 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    14041 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    14101 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    14161 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    14221 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    14281 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    14341 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    14401 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga
    14461 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    14521 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    14581 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    14641 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    14701 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    14761 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    14821 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    14881 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    14941 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    15001 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    15061 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    15121 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    15181 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    15241 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    15301 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    15361 cgtgatgtcg cgttataccg gtattgatga gattggccgt aaggaagggg cgatcggcgt
    15421 gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct
    15481 gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg ttcacgccgc
    15541 atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa
    15601 atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg ggcgcggctt
    15661 tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca ttttttaact
    15721 cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga gtccccaacg
    15781 cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga tcgttttggt
    15841 aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt tctgccatcg
    15901 ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa
    15961 atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac gtgcggatca
    16021 gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca tgaaatagcg
    16081 gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg agaacggaat
    16141 actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc aggttcgcac
    16201 ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc caaactgctg
    16261 ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat ccagcaattg
    16321 cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg ctgtaccagg
    16381 cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat tccaggcgct
    16441 ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac tggtgtaacg
    16501 tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc aaagcttccc
    16561 ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat accgagccag
    16621 ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc tacgcgcggt
    16681 attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag
    16741 ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt caccgatgcg
    16801 ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca aaatctcttt
    16861 cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac gccattcatc
    16921 tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc
    16981 ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg agcgcataat
    17041 ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca aacgccaggc
    17101 gacgatatcg cgcagctcct gaatctcatc ttcattaagc tgctgcgcct gggcaagcga
    17161 cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa acgccaccgc
    17221 cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca gtaccgtatt
    17281 agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg caggcatctg
    17341 ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac ggatacgctc
    17401 taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata acttgtcaca
    17461 ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc aggcttcttc
    17521 actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg tagttccggg
    17581 cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca gttcattgac
    17641 gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt
    17701 gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt ccaggtaggg
    17761 ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca tctgtcgatt
    17821 atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg
    17881 cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc aggtaacttg
    17941 tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag cgtcataccg
    18001 cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga cactttttta
    18061 cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc tacacttcgc
    18121 ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc tcaattcgtt
    18181 tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc
    18241 tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag
    18301 tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg
    18361 cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc ggaacacacc
    18421 gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa acgcctggat
    18481 gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc cgctgaacaa
    18541 ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt acagctggag
    18601 cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa cctctccggt
    18661 ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg
    18721 ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga acgcttcctg
    18781 cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct cgataacgtt
    18841 gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg taactactcc
    18901 tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt
    18961 cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg ccgccagtcg
    19021 aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata ccagaaacgt
    19081 aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa agtgctggaa
    19141 gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct gagcttctcg
    19201 atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg
    19261 ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt gggtgaaacg
    19321 gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa aaccgtttgg
    19381 gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat gccaagccgc
    19441 gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt tggcgaactt
    19501 tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg
    19561 ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac
    19621 gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac
    19681 cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt cttcgaaggt
    19741 aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc gctggagccg
    19801 aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat gcggcgcgaa
    19861 cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt aggcctgata
    19921 agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg cgtgaacacc
    19981 ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc cgtgcaggtt
    20041 ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac tcatcaaaaa
    20101 aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc tcccccatca
    20161 tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc
    20221 gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc
    20281 gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca
    20341 gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac ggatgctcac
    20401 gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca ataaacgcca
    20461 ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac gcgattttat
    20521 cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag acataatcgc
    20581 ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt accagggtgg
    20641 atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac ggcttcactt
    20701 cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca ccgctgatac
    20761 tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt tccatatact
    20821 gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc ttttcagcca
    20881 taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc tccatgccct
    20941 cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc aatagccagc
    21001 gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt
    21061 cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct
    21121 ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg acaccaatgg
    21181 ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc agcttttgcg
    21241 cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc
    21301 tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat
    21361 caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc
    21421 gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggtacgtt
    21481 agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa tccgctcctg
    21541 gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc
    21601 ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaagtcggc
    21661 gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct gatcggccat
    21721 ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt tttgttccag
    21781 ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg cctgaatctc
    21841 caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg aggtcacttc
    21901 atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgttcggt
    21961 catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt
    22021 gcgaaaacga gagtcggcat cgccatccag aagcaccgaa cagtcgatac agtgttccag
    22081 cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga caatcgccac
    22141 accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct gcgccacgct
    22201 accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa ttaccatcag
    22261 cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt ccgacaacat
    22321 attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga
    22381 ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag
    22441 caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac ttcctggcac
    22501 cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc
    22561 ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac cggcatagcc
    22621 gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt
    22681 gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg ccccgcattc
    22741 attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc
    22801 gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga ggatgcagaa
    22861 tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca cttttggctt
    22921 ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat tagctccatc
    22981 gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt
    23041 tttcgctttg tactgtgcgt ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc
    23101 catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc gcaggtattc
    23161 agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt
    23221 gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat
    23281 attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag
    23341 ttcgccgcgc atcgcccgtt cggttacctc cgctaccatc tcgcccgttc cggccagctt
    23401 ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa
    23461 acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct gcgcttcgtg
    23521 ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct gataatcttc
    23581 cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt attgggtcag
    23641 acgctgttta tccagcccac gaccatacag cagccagccg ctacgagctg cgtggtaatc
    23701 cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata aagagacatc
    23761 ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat cgttcgaagc
    23821 agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa
    23881 attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca tcgggcagtg
    23941 attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggagcgtc atggtttagt
    24001 caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac cctggcgcgt
    24061 caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt
    24121 caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac
    24181 gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga tcggctggcg
    24241 ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc aattgcgggt
    24301 aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct cgccaccgaa
    24361 gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct ctcactggca
    24421 atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg gcagcaatcg
    24481 cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga gaaaaaagag
    24541 tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catctctgac
    24601 tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca aatgcccgcc
    24661 acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg tcgcgcgcag
    24721 aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcgcgg
    24781 cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt tacctttatg
    24841 gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa tgcgctgaac
    24901 gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa aaccgtcgaa
    24961 ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg cttccaccac
    25021 ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa tccggataaa
    25081 aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa cctcaccgag
    25141 ctgctgccgg ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc
    25201 cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca
    25261 aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg
    25321 gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac
    25381 gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg
    25441 ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa
    25501 gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg
    25561 acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat
    25621 gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc
    25681 ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt
    25741 aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat
    25801 caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg
    25861 gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt attttatttt
    25921 ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact
    25981 aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac
    26041 aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag
    26101 gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg
    26161 cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc
    26221 atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag
    26281 tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct
    26341 gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta
    26401 aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc
    26461 agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg
    26521 aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc
    26581 attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg
    26641 ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg
    26701 cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc
    26761 attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc
    26821 agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc
    26881 cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata
    26941 ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt
    27001 ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac
    27061 ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg tggccccagg
    27121 aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct
    27181 ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac
    27241 cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat
    27301 caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg tgacggttac
    27361 cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga
    27421 tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat
    27481 ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga
    27541 aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg
    27601 aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca
    27661 gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga
    27721 caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc
    27781 atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag
    27841 aaccttcgtg tgctttcgcc agccccagac ggatcagatt tggcagattg agcggccctt
    27901 tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag
    27961 ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca
    28021 ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca
    28081 gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt
    28141 tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt
    28201 ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca
    28261 tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca
    28321 ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat
    28381 cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga
    28441 agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt
    28501 tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg
    28561 agatcagcat ctccacgcac agcaccatcg tgacatcaaa cagacgcggg ttacgatact
    28621 cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca
    28681 gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc
    28741 caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac
    28801 ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg
    28861 aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac
    28921 gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa
    28981 taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt
    29041 tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc
    29101 cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg
    29161 agtgtttatc aacaatcggg ccatccagat gcaggctttt ccagtcgaga acggttcctg
    29221 aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa
    29281 tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga
    29341 taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt
    29401 cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat
    29461 gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg
    29521 catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca
    29581 gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag
    29641 cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag
    29701 tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca
    29761 cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg
    29821 ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt
    29881 cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag
    29941 cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc
    30001 ggtacgggaa taccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg
    30061 ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga
    30121 tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga
    30181 tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt
    30241 tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc
    30301 tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg
    30361 cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa
    30421 ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc
    30481 aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct
    30541 tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat
    30601 gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag
    30661 cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa
    30721 gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac
    30781 aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg
    30841 gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac
    30901 cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa
    30961 ccgcatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt
    31021 tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg
    31081 cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc
    31141 tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa
    31201 tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc
    31261 tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta
    31321 cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc
    31381 actctaccgc actactgcca gcagcagatc gccatcatcg atacgcggtg tgaattcctc
    31441 tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct
    31501 gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt
    31561 gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat
    31621 cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa
    31681 aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc
    31741 gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg
    31801 catcatgctt attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa
    31861 cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa
    31921 tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg
    31981 tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa
    32041 gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag
    32101 cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg
    32161 tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac
    32221 gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca
    32281 gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct
    32341 gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct
    32401 tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagt
    32461 cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact
    32521 gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa
    32581 attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca
    32641 tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga
    32701 gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca
    32761 acacatcgaa gaactggcgg cgtttatcaa gggacttgga gatgttccgg ttcgcctgaa
    32821 cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga
    32881 agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc
    32941 ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg
    33001 ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac
    33061 gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc gttaagcgtc
    33121 gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa
    33181 cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg
    33241 ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc
    33301 cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg
    33361 gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta
    33421 cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc
    33481 cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt
    33541 gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt
    33601 cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga
    33661 tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa
    33721 gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg
    33781 gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa
    33841 caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa
    33901 taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc
    33961 ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt
    34021 atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag
    34081 atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg
    34141 accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc
    34201 gatactatgt aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt
    34261 ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag
    34321 cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta
    34381 gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc
    34441 ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga
    34501 cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt
    34561 ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata
    34621 aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct
    34681 tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa
    34741 tggatcaaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg
    34801 ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc
    34861 gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg
    34921 ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa
    34981 cggcgcaata ttaccaagcg ttacaggtat tctctgcccc acttctgcct tcctgttttt
    35041 gttgtgaaag aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc
    35101 cgctggactg gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc
    35161 ccatgaacaa actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc
    35221 ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca
    35281 gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt
    35341 gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc
    35401 gacaaagttc gggaggacga attacgacga attacttagt tttcagatca tttttaacgc
    35461 ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt gcctgagaat
    35521 ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact ttacgtgaag
    35581 ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg
    35641 cgtagtcctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg
    35701 tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc tgactttcaa
    35761 cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga
    35821 tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg tcatccatga
    35881 aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct
    35941 gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca
    36001 gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct
    36061 cgtaatttga gcctagcaac acgaggttgc attgctgaat agggagagac ttcaccctct
    36121 acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac cattacagtc
    36181 tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta
    36241 cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat atagatcaca
    36301 attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca
    36361 ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac cgcgccctgg
    36421 aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac gttcctgtgc
    36481 caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc
    36541 cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg cacattctac
    36601 ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt attcgctctt
    36661 caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga tcagatcgtt
    36721 gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt
    36781 gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggtgc
    36841 gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg
    36901 aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt caacgtgcga
    36961 acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac
    37021 ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa acgccacgcg
    37081 gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttatc
    37141 ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag gcgcccactc
    37201 caccaggcca ttcagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac
    37261 cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg cgcctttaac
    37321 cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag catcgagatc
    37381 cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt taccgctctg
    37441 atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc
    37501 aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt catcgagcag
    37561 ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc
    37621 gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc
    37681 gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc
    37741 ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac
    37801 agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg acttagcgtg
    37861 ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc cgaacagcag
    37921 caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg caaaagtgcg
    37981 gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt cttgagtaag
    38041 cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc atcaacgggg
    38101 gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag aaacggtcca
    38161 ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag
    38221 cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg
    38281 tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct tattcggttt
    38341 ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca ggcccgtccg
    38401 ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga ttttagcgtt
    38461 gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga tttccgccat
    38521 cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt cggcccagct
    38581 ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat aatccaccca
    38641 cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg tgacgctgta
    38701 atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc
    38761 atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact gattggcaac
    38821 gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc
    38881 gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga ggcacggact
    38941 tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg ttccagcagc
    39001 gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa caatgtagct
    39061 tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg cgttaactga
    39121 aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca cggaaaggcg
    39181 tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc
    39241 gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa
    39301 ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt cgtcagtacc
    39361 caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt ctggcgtcag
    39421 ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc
    39481 agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg ggctcgcaat
    39541 ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa tgcccagttg
    39601 ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa
    39661 atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc agtatcaatt
    39721 gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg tcacagtgat
    39781 gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct gcccgcgcgt
    39841 ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca ggtgttaagc
    39901 tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat
    39961 tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt ccagttgatg
    40021 aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga gaaccgcagc
    40081 ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa agtcgacagc
    40141 gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt tgatgcggat
    40201 aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt
    40261 agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac tccgcacacg
    40321 aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc
    40381 cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt agaagccagc
    40441 cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc
    40501 gaggtgaaag gttgttttga tatgatcatc tccaacccgc cgttccacga tgggatgcaa
    40561 accagcctgg atgcggcgca aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc
    40621 ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct ggatgagaca
    40681 tttggcttcc atgaagttat cacgcaaacc gggcgcttca aggtatatcg cgccattatg
    40741 acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc gtgtcggatg
    40801 cggcgtgaac gccttatccg acctacaaaa cctgcacgtt agcccttcgt aggccagata
    40861 agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag
    40921 tttcctgatg gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa
    40981 agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatggtat
    41041 gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg
    41101 ggggttcaag tccccccctt cgcaccaaaa accacgttga tattgctcgc actgggcgaa
    41161 ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gttcttacgg acgtgggggt
    41221 tcaagtcccc cccctcgcac caaacgaggc gatatcaaaa aaagtaagat gactgtgcga
    41281 aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg gacgtggggg
    41341 ttcaagtccc ccccctcgca ccaattatct ttacttcctt tcttgtttct tccctgtttt
    41401 gtcatccctg attcaaattc attatcatca gcatgatgcc agcaataagc gccaggcacg
    41461 atggtagcaa aatgtaatgc cgcaactgtt tgtgatacag catcaccggc gtcaatacta
    41521 atcccatcag aatgacaatc cgccactgat ccggcgataa atccgcatac atcagccctg
    41581 caatcagcaa tcccggcagc aacactcccc agccactgcc cagcgtacgt tgcaacatgg
    41641 tttcatctct ttcattgata atgaaaacca atatcatatg ataattttta tcatttgcaa
    41701 gccagagaaa tcccttgcta tcgagtaaac ctatcgatag gattagcaat cattatcatt
    41761 tagattacta tcacgattat ggcctatcgt tccgcaccgc tctatgaaga tgtcatctgg
    41821 cgaacgcatc tccagccgca ggatgctggg cttgcacaag ccgtacgggc gatgatcgcc
    41881 aaacatcgtg aacatttgct ggagtttatc cgcctggatg aacccgctcc gcttaacgcc
    41941 atgacgctgg cgcaatggtc atcaccgaat gcgctaagtt ctctactggc ggtctattcc
    42001 gatcatatct atcgcaacca accaacgatg atccgcgaga acaaaccgct gatctcacta
    42061 tgggcacaat ggtatatcgg cctgatggtg ccaccattaa tgctggcgct actgacgcag
    42121 gaaaaagcat tagatgtgtc gccggaacat ttccatgctg agtttcacga aaccggacgc
    42181 gtcgcctgtt tctgggtcga tgtgtgtgaa gataaaaacg caacaccaca ttcgccgcag
    42241 cagcgaatgg aaacgttaat cagccaggcg ctggttccgg ttgtgcaagc actagaagcg
    42301 accggagaga tcaacggcaa acttatctgg agtaataccg gttatttgat caactggtat
    42361 ctcactgaga tgaagcaact gctcggcgag gctactgttg aatcgctgcg ccatgccctc
    42421 ttttttgaga aaacgctcac gaacggtgaa gataatccac tctggcgtac cgtggtactg
    42481 cgcgacggcc tgctggtgcg ccgcacttgc tgccagcgtt atcgcttacc ggatgtgcag
    42541 caatgtggct attgtacgct gaaatgaaag cactgccggg gaagtaaacc cggcatcatg
    42601 cggattaata gggatgcaac acattacttg cttcgcaatg ccgcatacat aacagaatat
    42661 ctgcggcctc caacaaccct gcatcagaac tgacgcccag tttcgacatc acattaaatt
    42721 tatgcgctcg gatcgttttg atattacgtt taagctgctc ggcaatttgt gtcattgagt
    42781 agccacgcga cataaagcgc aatatttctc tctccgtcgg gcttaacgtc cggctttggt
    42841 taatgtacca ctgattgttc agtcggtcgg tcgcctgacg tacaccattt aatgacaaaa
    42901 agaattcctg atgaaaaatc tccagcgttg acgctttact taatacaccg tccagcggtg
    42961 atggcgacaa tgaaccaatc agccgagctt caatatcatc atccacaata actaaacgcc
    43021 gggtacgcgg aaacttaatc gccagctcag tcaggcaaga taatccctct ctccgctcac
    43081 ttctcatggc agaaaaagaa aaaatgaccg ccgcaaacga gatccgcgac atggcatcct
    43141 gaaatgactc ctgactggta taggtatgga gcttataagg gttgcccgca aactttctga
    43201 ataaactctc aataccgata ctactcatga cgcatttttc tatgagtgcg acatttctct
    43261 tcttaattcg gctgtgttcc attctgcggc atctccataa acagaaggca agttaagttc
    43321 tcttgcaccc tgcgcgctat ttatccatgc atacatttca gcgttgctgt gaattgccag
    43381 tcgacgcatc gcactgtttt tttgggcgct gatagtttta ttacttttct taagcaatga
    43441 agctatctgg ttgatgcccc atcctttacc taatagccgc agtaccttgc gctctgaaag
    43501 agtgagaatg acggagcgat agctaaaatc atgagtttca gtcattgccg gagaggtcag
    43561 catggcgctg atacgctctg catgcgtatt gccggaacgt acggtcttga ccagattctc
    43621 aatg
//
LOCUS       DAJTYI010000016        43172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYI010000016 DAJTYI010000000
VERSION     DAJTYI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..414
                     /locus_tag="OO564_000898"
     CDS             <1..414
                     /locus_tag="OO564_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6652124.1"
                     /translation="IMYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIK
                     LFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPF
                     DKYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            439..3038
                     /locus_tag="OO564_000899"
                     /pseudo
     CDS             439..3038
                     /locus_tag="OO564_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3029..4099
                     /locus_tag="OO564_000900"
     CDS             3029..4099
                     /locus_tag="OO564_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6652125.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4111..4653
                     /locus_tag="OO564_000901"
     CDS             4111..4653
                     /locus_tag="OO564_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6652126.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4661..5176
                     /locus_tag="OO564_000902"
     CDS             4661..5176
                     /locus_tag="OO564_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6652127.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5142..5879
                     /locus_tag="OO564_000903"
     CDS             5142..5879
                     /locus_tag="OO564_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6652128.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5990..7000
                     /gene="pyrD"
                     /locus_tag="OO564_000904"
     CDS             5990..7000
                     /gene="pyrD"
                     /locus_tag="OO564_000904"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6652129.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7174..7716
                     /gene="zapC"
                     /locus_tag="OO564_000905"
     CDS             7174..7716
                     /gene="zapC"
                     /locus_tag="OO564_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6652130.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7713..8822)
                     /gene="ycbX"
                     /locus_tag="OO564_000906"
     CDS             complement(7713..8822)
                     /gene="ycbX"
                     /locus_tag="OO564_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6652131.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9066..11174
                     /gene="rlmKL"
                     /locus_tag="OO564_000907"
     CDS             9066..11174
                     /gene="rlmKL"
                     /locus_tag="OO564_000907"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6652132.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11186..13093
                     /locus_tag="OO564_000908"
                     /pseudo
     CDS             11186..13093
                     /locus_tag="OO564_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13223..14476
                     /gene="pqiA"
                     /locus_tag="OO564_000909"
     CDS             13223..14476
                     /gene="pqiA"
                     /locus_tag="OO564_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6652133.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14481..16121
                     /gene="pqiB"
                     /locus_tag="OO564_000910"
     CDS             14481..16121
                     /gene="pqiB"
                     /locus_tag="OO564_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6652134.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16118..16681
                     /gene="pqiC"
                     /locus_tag="OO564_000911"
     CDS             16118..16681
                     /gene="pqiC"
                     /locus_tag="OO564_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6652135.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16937..17104
                     /gene="rmf"
                     /locus_tag="OO564_000912"
     CDS             16937..17104
                     /gene="rmf"
                     /locus_tag="OO564_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6652136.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17174..17692)
                     /gene="fabA"
                     /locus_tag="OO564_000913"
     CDS             complement(17174..17692)
                     /gene="fabA"
                     /locus_tag="OO564_000913"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6652137.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17761..19521)
                     /locus_tag="OO564_000914"
     CDS             complement(17761..19521)
                     /locus_tag="OO564_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6652138.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19706..20158
                     /gene="matP"
                     /locus_tag="OO564_000915"
     CDS             19706..20158
                     /gene="matP"
                     /locus_tag="OO564_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6652139.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20234..21280)
                     /gene="ompA"
                     /locus_tag="OO564_000916"
     CDS             complement(20234..21280)
                     /gene="ompA"
                     /locus_tag="OO564_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6652140.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21636..22145)
                     /gene="sulA"
                     /locus_tag="OO564_000917"
     CDS             complement(21636..22145)
                     /gene="sulA"
                     /locus_tag="OO564_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6652141.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22364..22993
                     /gene="sxy"
                     /locus_tag="OO564_000918"
     CDS             22364..22993
                     /gene="sxy"
                     /locus_tag="OO564_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6652142.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22956..25118)
                     /gene="yccS"
                     /locus_tag="OO564_000919"
     CDS             complement(22956..25118)
                     /gene="yccS"
                     /locus_tag="OO564_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6652143.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25128..25574)
                     /locus_tag="OO564_000920"
     CDS             complement(25128..25574)
                     /locus_tag="OO564_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6652144.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25697..27751
                     /gene="helD"
                     /locus_tag="OO564_000921"
     CDS             25697..27751
                     /gene="helD"
                     /locus_tag="OO564_000921"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6652145.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27783..28241)
                     /gene="mgsA"
                     /locus_tag="OO564_000922"
     CDS             complement(27783..28241)
                     /gene="mgsA"
                     /locus_tag="OO564_000922"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6652146.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28337..28999)
                     /locus_tag="OO564_000923"
     CDS             complement(28337..28999)
                     /locus_tag="OO564_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6652147.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29172..29585
                     /locus_tag="OO564_000924"
     CDS             29172..29585
                     /locus_tag="OO564_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6652148.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29630..29947)
                     /gene="hspQ"
                     /locus_tag="OO564_000925"
     CDS             complement(29630..29947)
                     /gene="hspQ"
                     /locus_tag="OO564_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6652149.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30005..31195)
                     /gene="rlmI"
                     /locus_tag="OO564_000926"
     CDS             complement(30005..31195)
                     /gene="rlmI"
                     /locus_tag="OO564_000926"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6652150.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31290..31568
                     /gene="yccX"
                     /locus_tag="OO564_000927"
     CDS             31290..31568
                     /gene="yccX"
                     /locus_tag="OO564_000927"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6652151.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31565..31894)
                     /gene="tusE"
                     /locus_tag="OO564_000928"
     CDS             complement(31565..31894)
                     /gene="tusE"
                     /locus_tag="OO564_000928"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6652152.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31985..32644)
                     /gene="yccA"
                     /locus_tag="OO564_000929"
     CDS             complement(31985..32644)
                     /gene="yccA"
                     /locus_tag="OO564_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6652153.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32851..32938)
                     /locus_tag="OO564_000930"
     tRNA            complement(32851..32938)
                     /locus_tag="OO564_000930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32902..32904),aa:Ser,seq:tga)
     gene            33364..34482
                     /gene="hyaA"
                     /locus_tag="OO564_000931"
     CDS             33364..34482
                     /gene="hyaA"
                     /locus_tag="OO564_000931"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6652154.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34479..36237
                     /gene="hyaB"
                     /locus_tag="OO564_000932"
                     /pseudo
     CDS             34479..36237
                     /gene="hyaB"
                     /locus_tag="OO564_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36256..36963
                     /gene="hyaC"
                     /locus_tag="OO564_000933"
     CDS             36256..36963
                     /gene="hyaC"
                     /locus_tag="OO564_000933"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6652155.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36960..37547
                     /locus_tag="OO564_000934"
     CDS             36960..37547
                     /locus_tag="OO564_000934"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6652156.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37544..37942
                     /gene="hyaE"
                     /locus_tag="OO564_000935"
     CDS             37544..37942
                     /gene="hyaE"
                     /locus_tag="OO564_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6652157.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37939..38796
                     /locus_tag="OO564_000936"
     CDS             37939..38796
                     /locus_tag="OO564_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6652158.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38930..40474
                     /gene="appC"
                     /locus_tag="OO564_000937"
     CDS             38930..40474
                     /gene="appC"
                     /locus_tag="OO564_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6652159.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40486..41622
                     /gene="appB"
                     /locus_tag="OO564_000938"
     CDS             40486..41622
                     /gene="appB"
                     /locus_tag="OO564_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6652160.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41634..41726
                     /gene="cbdX"
                     /locus_tag="OO564_000939"
     CDS             41634..41726
                     /gene="cbdX"
                     /locus_tag="OO564_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6652161.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41806..43104
                     /gene="appA"
                     /locus_tag="OO564_000940"
     CDS             41806..43104
                     /gene="appA"
                     /locus_tag="OO564_000940"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6652162.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT        10743 a        10503 c        11499 g        10427 t
ORIGIN      
        1 attatgtaca ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta
       61 cgggcagtcc cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa
      121 agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg
      181 gcgaaactgc gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat
      241 ttcattaccc tgacagacct gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc
      301 ccctttgata aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat
      361 cgaaccatca atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc
      421 ttcagttaag agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt
      481 ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa
      541 gctgaaacct ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg
      601 tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg
      661 aatcagacaa ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg
      721 attgccgcct gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt
      781 ccggctttta aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat
      841 gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc
      901 atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg
      961 ctattactgg gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat
     1021 tctggcgaca gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg
     1081 cgcaacaatt caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc
     1141 agctatttgc agcgggcggt tattccacta aaaggcgaac tgaccgtagg cgatgattat
     1201 actgcaggcg attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac
     1261 aacatgctgc cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc
     1321 aatgcccaga taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct
     1381 ggtgcttttg aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa
     1441 atcaaagaag cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta
     1501 ctccagcgtc aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt
     1561 aatgaacagc aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga
     1621 acgacatggt atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg
     1681 ggatttaacc ttggtgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg
     1741 ctggcagagc aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc
     1801 aaccaattgg gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac
     1861 accctttccg acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat
     1921 gaagatacgc cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg
     1981 caggtcaata tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag
     2041 caaacttact ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa
     2101 attaaagatc tctcgctggg ggtttcctgg aactacagta agtcccgtgg tcaacctgat
     2161 gctgatcagg tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt
     2221 aatgatagct ataccaggaa aaaaattacg cctggatgac ctctaacacc agtatcgata
     2281 acgaagggca cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc
     2341 tgagctacag cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca
     2401 gcatggacta caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg
     2461 gcagtcaaca acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca
     2521 ttaccctggg gcaatcgctg ggggaaacta acgtgctgat tgtagcacca ggcgcagaga
     2581 atactcgtgt ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc
     2641 cctatgccac ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta
     2701 acgtggatct tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg
     2761 cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac
     2821 cgctgcgttt tggcgcgata gcgacgctgg acggcataca gactaatagc ggcattattg
     2881 atgatgatgg ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct
     2941 ggggcgaagc tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta
     3001 actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt
     3061 attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta
     3121 tatcgggcag tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa
     3181 aactctggat gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa
     3241 cgaatcgagt gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg
     3301 atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa
     3361 tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc
     3421 tttcgaaaac gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca
     3481 acgtttaggt ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca
     3541 gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta
     3601 caacataccc gcaggtgatt cctgcacgac tactggtaca ccggttttag tgtattacct
     3661 gtctggtacg atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt
     3721 tgatctgggc gacgtatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc
     3781 cagaacggca gaacttgcaa ttccggtcag gtgtaacacg ggaaacgcag ggttagttaa
     3841 cgtcaatctg agtctgacgg caacctcaga ccccagctat ccccaggtga ttaagacgtc
     3901 acgtcctggc gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg
     3961 tgggacacta ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta
     4021 tccagtcagc acgacaggcg taccaccaga aaccgggcga tttgaagcca cggcaacggt
     4081 gagaataaat tttgattaac gcgtgaacgt atgaagaaaa aaacgatata tcagtgcgtt
     4141 attttgttct ttagccttct taacatccat gtcgggatgg ctgggcctga acaagtcagt
     4201 atgcatattt atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta
     4261 caaaatattc atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc
     4321 actgctgctg atttgaatat tgatatcacc ggttgtgccg ctggtattac tggcgcgggc
     4381 gtccttttta gcggcgaggc tgacaccctt gcgccgacac tgctcaaact aactgacaca
     4441 ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag
     4501 caagaaatcc agctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca
     4561 ctcaaatatc aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg
     4621 gcggttctct attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg
     4681 gattctgttc ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt
     4741 caacattagc ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat
     4801 caatgtgcag ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga
     4861 gaaatctttc atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgatgtcac
     4921 cttttcagga accctcgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga
     4981 tgcagccagt gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa
     5041 ccaggcgagt aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca
     5101 attgcgacct gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt
     5161 acttcattat gactaatacg tggaacagat tagcgctctt gattttcgcc gttttatcgc
     5221 tgctggtggc gggtgaattg caggcaggag tggtggtcgg cggaacgcga tttatctttc
     5281 cggcagacag agaatcgata tctattttac tgactaatac cagtcaggaa tcttggctta
     5341 ttaacagcaa aatcaatcgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag
     5401 cactattact ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc
     5461 gcttgctgag aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa
     5521 gcattgccag cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct
     5581 cggtatttaa actgttctgg cgacacgaag gtttgccggg cgacccgctg gaagcttatc
     5641 aacaattacg ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact
     5701 acattaacct gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagtcgtgc
     5761 cgcctaaaag ccagcgtcag acaaactggt gtcaggtaat agcaccgtgt catgtcgcct
     5821 ggcggacgat aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa
     5881 acaggttctg aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac
     5941 tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc
     6001 cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca
     6061 gcaattacgc cgcattaccg gaacaccgtt tgaagcactg gtgcggcaga aagtgcctgc
     6121 gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct
     6181 tgataaagac ggggagtgca ttgatgcgtt aggcgcgatg ggatttggat cgatcgagat
     6241 cggtaccgtc acgccacgtc cacagcctgg taatgacaag ccgcgtctct ttcgtctggt
     6301 agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt
     6361 agagaacgta aaaaaggccc attatgacgg cgtcttgggt attaatatcg gcaaaaataa
     6421 agatacgcca gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc
     6481 ctatgcggga tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct
     6541 gcaatatggt gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt
     6601 gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga
     6661 agacgaattg atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc
     6721 aaccaatacc actctcgatc gttctctggt tcagggaatg aaaaattgcg atcaaaccgg
     6781 tggcttaagt ggtcgtccgc ttcagttaaa aagcacagaa attattcgcc gcttgtcact
     6841 agaattaaac ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc
     6901 gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa
     6961 aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct
     7021 ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct
     7081 tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcaaca atggattgtg
     7141 ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg
     7201 cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta
     7261 tttcgctcac gttttgcgcg caaaatgttg actccagatg ccttttctcc cgcaggcttt
     7321 tgcgttgatg atgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta
     7381 tctaaagacc aaaaagccga actggtgctg aatgcactgg tagcaattcg ttatctcaaa
     7441 ccgcaaatgc cgaaaagctg gcattttgtc tcccatggtg aaatgtgggt gcccatgccg
     7501 ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt
     7561 gaatctggcg aaaatgccgc gctatgcctg ctggcacaac cctgcgttgt tattgcgggt
     7621 cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtg
     7681 aatgttgaca gcttcagcct cgaacaggca gtctaacgcg tcagcttaag ggcagtcttc
     7741 ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc
     7801 gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa
     7861 taagggatac gaattccctg attttctaat tgctccagca acacttgttg gttatttcca
     7921 cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg
     7981 gcggtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc
     8041 acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg
     8101 acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc
     8161 ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta
     8221 accacatcaa acaccacatc accaatgcga atcactttcc agctatcttc ttcccaggct
     8281 gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa
     8341 cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca
     8401 aatgacagag gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag
     8461 cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca
     8521 attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca
     8581 gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca
     8641 ggtgaaggag taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca
     8701 ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt
     8761 gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc
     8821 acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc
     8881 tataatgcgc aacaattttc ttagcgcctg tcccgttagg ctattttatt tgtcagtttg
     8941 gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctct
     9001 ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg
     9061 acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg
     9121 aactggaaaa tctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg
     9181 gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt
     9241 tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga
     9301 tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga
     9361 atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg
     9421 ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg
     9481 ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt
     9541 tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag
     9601 ccgctattgt gatgcgatcc ggctggcagt caggaacacc gctgctcgat ccgatgtgtg
     9661 gttccggtac gttgctgatt gaagcggcga tgctggcgac cgatcgcgca ccaggcttgc
     9721 accgtgggcg ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag
     9781 tgaaagcgga agcgcaaact cgcgcccgta aaggcctggc tgagtatagc tctcattttt
     9841 acggttcgga cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg
     9901 ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc
     9961 cgaaagggcc gtacggtaca gtgttgagca acccgccata cggtgaacgt ctggacagcg
    10021 aaccggcgct gattgcgctg catagtctgc tgggccgaat catgaaaaac cagttcggtg
    10081 gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcctg cagctgcgtg
    10141 cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg
    10201 ttgccgaaag cacaccagac agtaaaccgg caatggtagc ggaagattac gccaaccgcc
    10261 tgcgtaagaa cctcaaaaaa ttcgagaagt gggcacgcca ggaagggatt gaatgttacc
    10321 gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg
    10381 tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg cgtcagcgtc
    10441 tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc
    10501 tgaaaacccg tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg
    10561 agtttcttga agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg
    10621 atactggtct gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag
    10681 gcaaagattt cctcaacctg ttttcttata ccggcagcgc caccgtgcat gcgggattag
    10741 gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggaa tgggcagaac
    10801 gcaacctgcg tctgaatggc ctgactaggc gtgcgcatcg cctgattcag gccgattgcc
    10861 tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct
    10921 tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga
    10981 tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac
    11041 gtggcttccg tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta
    11101 cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga
    11161 ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc
    11221 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt
    11281 ttgtctggtg ggccgcaacg gcgcaggaaa atcgacgtta atgaaaatcc tcaaccgtga
    11341 acaagggctg gatgacggtc gcattattta tgagcaagat ttgattgtag ctcgtctgca
    11401 acaggatccg ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga
    11461 agaacaagcg gaatatctga aacgctatca cgatatttcg tgcctggtga tgaacgaccc
    11521 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct
    11581 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt
    11641 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
    11701 gagcaatccg cgcgtattgc tgctggacga accgactaac catctggata tcgaaaccat
    11761 cgactggctg gaggggttcc ttaaaacctt caatgggacg attattttca tctcccacga
    11821 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
    11881 gacctatcca gggaattacg accagtacct gcaggaaaaa gaagaagccc tgcgcgtgga
    11941 agaactacaa aatgccgagt tcgatctcaa actggcgcag gaagaagtgt ggattcgcta
    12001 ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg
    12061 tcgcgaacgt ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc
    12121 cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa
    12181 gcaactggtg aaagattttt ctgcccaggt tctacgtggc gacaaaattg ccctgattgg
    12241 tccgaatggg tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcggg
    12301 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
    12361 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
    12421 ggttaatggc aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa
    12481 acgggcgatg acgccagtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc
    12541 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
    12601 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
    12661 ggttagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga tcttcgaagg
    12721 cggcggtaaa attggtcgtt atgttggcgg ttatcatgat gcccgtggtc agcaagaaca
    12781 atatgtggca ctcaaacagc ctgcggtgaa aaaaagcgaa gaagccgccg cgccaaaagc
    12841 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca
    12901 gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc
    12961 ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ctgatatggc
    13021 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
    13081 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt
    13141 gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat
    13201 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
    13261 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
    13321 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
    13381 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
    13441 gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc
    13501 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
    13561 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
    13621 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
    13681 gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
    13741 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
    13801 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg
    13861 cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
    13921 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
    13981 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
    14041 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
    14101 cattctgtta tggagcgaag gctcttatcc cgtcgctgtg gtgatctttc tggccagtat
    14161 tatggtgcca acgttaaaga tgagcgccat cgcgtggctg tgttgggatg ccaaagggca
    14221 tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
    14281 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
    14341 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
    14401 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
    14461 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
    14521 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc
    14581 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
    14641 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
    14701 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
    14761 tggaaaaatt gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg
    14821 aagggattag cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg
    14881 cgaaaggcag caaaatggat aaatacgatt tgctggactc accaccgttg gccccgcctg
    14941 atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag
    15001 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc
    15061 aaaaacgcaa tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca
    15121 gcaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc
    15181 gtgtggagat gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg
    15241 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
    15301 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
    15361 attcggtacg cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta
    15421 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
    15481 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
    15541 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
    15601 ccggaaacct ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg
    15661 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc
    15721 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga
    15781 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
    15841 tgcaaacgac gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc
    15901 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
    15961 gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
    16021 aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga
    16081 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
    16141 tgcagcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt
    16201 acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt
    16261 agagcaggtc actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
    16321 tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg
    16381 caacaccctg gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc
    16441 tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga
    16501 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
    16561 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
    16621 ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta
    16681 attataaaga tttgtaaata taaccgtctc cggtatgccg cctgaggcgg tttttttgtt
    16741 tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt
    16801 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
    16861 attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt
    16921 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
    16981 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
    17041 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc
    17101 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc
    17161 tgtctgatga taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg
    17221 cggtatagat cagacgacaa tcaaccagca cttcgccatc tgccagaccc ataatcagac
    17281 gacgattaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta
    17341 cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca
    17401 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    17461 taaccggatc gccaatgaag tggcatccga agaaccacag atccggattg atatccagtt
    17521 ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggttattttg accacacggt
    17581 ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgt
    17641 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    17701 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    17761 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    17821 ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    17881 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    17941 gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    18001 aacgttagca gtggggataa tgacaccttg tttccccgtt aactcacgtt gctggcaaat
    18061 agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctggacgc ggccgaactg
    18121 atcgactgaa cctgtgatag cgatactctg attcactggc acatcagcga gggcgcttat
    18181 cagggcgcag agttcagcca tcgaggcact atcgccatcc acttcactgt atgactgctc
    18241 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    18301 cgcctgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc
    18361 gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc
    18421 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    18481 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc
    18541 gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa
    18601 ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc aatgcgggct
    18661 aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca
    18721 agcatccgct cccggggcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    18781 ccactggctt actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    18841 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    18901 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    18961 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    19021 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    19081 atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag
    19141 ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    19201 tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt gatcgcccac
    19261 cagttggcct gcatcgcttt gtagcgtacg cgcggcatcg gcaataagat tcagatactc
    19321 agactcttcc ggggccttag ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    19381 ctccagcgca aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    19441 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    19501 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    19561 accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg ctgggcgtta
    19621 aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga
    19681 gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg
    19741 ttggaaatgg aagtatctgg taaagaagca tcgcgaaggg gagttaatta cccgttacat
    19801 agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctcgaaa atgaacccgt
    19861 gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca
    19921 gaccattcgg gccagacgga agcgccattt taatgcggaa caccagcata cgcgcaaaaa
    19981 atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcac agcgtcgcgg
    20041 taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata
    20101 cgcgaataaa atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact
    20161 gacgaaagtc agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct
    20221 accagacgat aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga
    20281 tctctacgcg acgatccgga gccaagcagt cgatcagtgc agcacgctgt ttcacgttgt
    20341 cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg
    20401 ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac
    20461 cctggttgta agcgtcagaa ccgatgcggt cagtgtaacc cagaacaact acggaaccgt
    20521 ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg
    20581 gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg
    20641 tctgtacttc cggtgccgga gctggagcaa ctaccggagc tgcttcgccc tgaccgaaac
    20701 ggtaggaaac acccaggctc agcagaccgt tgtccggacg agtaccgatg gtgtttgcgt
    20761 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt
    20821 actcaacacc acctgcgaaa accggagaaa cgccagtgtc gtggtcttta aaggatgcgc
    20881 caccaggtac gttagccttg gtgtctgcac gccataccat accacccaga cgagtgtaga
    20941 tgtccagatc gtcagtgatt gggtaaccca gtttagcggt cagctgaacg ccctgagctt
    21001 tgtatgcgcc gttgatgttg tcgcctttgt acggcatacg acctaaccag tcgtaaccca
    21061 tttcaaaacc aacatacggg ttaacctggt aaccaccaaa agcacctgca cccagttggt
    21121 tttcgtgggt cggaccattg ttaggaataa aaccagtgtc atggtactgg gaccagccca
    21181 gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac
    21241 cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc tcgttatcat
    21301 ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc accgggggat
    21361 ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa
    21421 agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat gaaaaaaaag
    21481 tcttgtataa ggtatgttta atctttttgt cagcgacaat ttacagaaaa aaaacgcgga
    21541 aaccgcttca gacaagcctt cgcaaggaaa attagtcacg actgaaagca ttggctgggc
    21601 gacaaaaaaa gttccaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt
    21661 tttagcccgg aaagttgtct cgttgtgtga gaggatgcgc ttaccggacg cataataaac
    21721 cccatagcgt taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa
    21781 tcatctgtca accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt
    21841 gactccactg tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc
    21901 ccagatgcct gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag
    21961 cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc
    22021 tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca
    22081 cgcgcaattt tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg
    22141 tacataatca atccagcccc tgtgagttac tgtatggatg tgcagtacat ccagtgacaa
    22201 caaagatcaa ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa
    22261 cataaattaa tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt
    22321 aatgtgaatc attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta
    22381 taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc
    22441 attgtttggc agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg
    22501 tgagttgtat cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt
    22561 ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga
    22621 aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc
    22681 gctgaaagag aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc
    22741 ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat
    22801 acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa
    22861 gattctgttt atgcttgaag gtgccattat cggcattcat gaagctgcgc tcccggtggc
    22921 acgccgccag gagcttgcag aatgggctga ctctcttacg ccgaaacagg agtttcctgc
    22981 ggagcttgag taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac
    23041 ccaacttgtt gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc
    23101 cgctgtttca ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt
    23161 aacgcgtcat caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt
    23221 aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac
    23281 agcaagcgaa acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt
    23341 gataccacag acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac
    23401 gccagacggt tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag
    23461 ttggcctctg tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcgggccag
    23521 atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt
    23581 aacgctactt caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg
    23641 aacatcgttg catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata
    23701 accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc
    23761 gcgataccta ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag
    23821 tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt
    23881 atttgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag
    23941 agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc
    24001 gggctgtcat cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct
    24061 gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt
    24121 gttttgagta aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata
    24181 tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag
    24241 cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc
    24301 acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca
    24361 cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga
    24421 ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc
    24481 agctgaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca
    24541 ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc
    24601 aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg
    24661 taccagacgg caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat
    24721 gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt
    24781 tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat
    24841 agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc
    24901 gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct
    24961 gccaccatcc ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac
    25021 gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccaa
    25081 gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga
    25141 cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca
    25201 accggccata gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg
    25261 attgaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag
    25321 aaaatcagcc agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca
    25381 gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta
    25441 gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga
    25501 gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc
    25561 aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa
    25621 cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt
    25681 gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca
    25741 cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga
    25801 ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg
    25861 cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg
    25921 cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat
    25981 gagcgaaatt gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg
    26041 agaaaataaa tggctgacgc gtgagcaaac ctctgacgtt cagcaacaaa tccgccaggc
    26101 tttgtcggcg ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc
    26161 gtggcgtaaa tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca
    26221 ggcgtatacc gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc
    26281 accgctgaat ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct
    26341 ggccggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc
    26401 gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga
    26461 agagatggac gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt
    26521 tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa
    26581 actggaaaat gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg
    26641 cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg
    26701 gtcagtgcca gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg
    26761 cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc
    26821 cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt
    26881 attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat
    26941 tcatcaggcg attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct
    27001 ggttgatgaa tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa
    27061 gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt
    27121 cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg
    27181 ctgtgattta gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt
    27241 tattcagcag aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa
    27301 aaaagctgtc acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg
    27361 ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc
    27421 cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca
    27481 tgccagcaaa gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtga
    27541 tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga
    27601 ggatttcccg gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca
    27661 tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa
    27721 tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa
    27781 tattacttca gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg
    27841 tcgttgaaat gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga
    27901 atgttccata ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca
    27961 tttagtggat cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca
    28021 acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg
    28081 cgggaaatca agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt
    28141 tgatgccgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca
    28201 atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa
    28261 ctttcctaca gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg
    28321 cacaggcaca aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc
    28381 gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag
    28441 actgcggtgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca
    28501 tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct
    28561 cgacctcata atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg
    28621 gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag
    28681 cttcccgctc attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat
    28741 taaaactgac caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa
    28801 tcgtcttctc aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg
    28861 caccgcgcag cagagagctg gaaacctttt tgccgtcgag taccagaaga tcgacatcgg
    28921 ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg
    28981 tcacgatgcc ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc
    29041 aaaatttttc tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat
    29101 tcattcgtat tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc
    29161 tggaggaaat aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg
    29221 cgctggtggg ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac
    29281 tcgaccaggg gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg
    29341 gccagcaggg gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt
    29401 tccgtaattc agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa
    29461 ccctctggat gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg
    29521 ggttaaatgt ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca
    29581 aataaaaaat ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca
    29641 gacgcggggc ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt
    29701 caggatgttc atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg
    29761 gtaggccgtt atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat
    29821 tcaccgccag ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga
    29881 ccactccgag ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg
    29941 caatcatagt cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt
    30001 caagttacat gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga
    30061 tcaccggatg atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat
    30121 caattgcggc atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg
    30181 agaaagtcag gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt
    30241 tataaccacg acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca
    30301 tcacgataac gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat
    30361 catcacggac aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg
    30421 caatatccag cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca
    30481 gtgccgatac ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa
    30541 cgtagcggcg ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc
    30601 cgtgctgaat atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg
    30661 gcaactcgcc ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa
    30721 catcgctgcg atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg
    30781 ccgcgcgctg atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac
    30841 gatcgatagt aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga
    30901 ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa
    30961 aaaaagcaat gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg
    31021 aagctggcga ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg
    31081 tttcaccgag gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat
    31141 ggcgacgaag taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt
    31201 acttttctgt cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa
    31261 cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt
    31321 acgggcgagt tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg
    31381 gcttaaccgg gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg
    31441 aagaagggca ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc
    31501 gtgtagaacg ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta
    31561 tccgttaaat acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg
    31621 ggcctttcgg gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat
    31681 tcgccatcgc ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt
    31741 cacgtacaaa acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg
    31801 caatcaccac tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat
    31861 ccgtttctat ctctttacct tcgaagatca gcataattat tactacctta atcagactgg
    31921 cggcagtgta acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt
    31981 gagattaatc gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca
    32041 gcgaaacata caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt
    32101 tgctggtttc aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca
    32161 gatgcagagc aggcagttgc aggaagatat tagcaaccat accaatcagt accaccacaa
    32221 tacccgccat cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg
    32281 cagagcagca gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca
    32341 ttccggcaga cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa
    32401 aggcgaatgc ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca
    32461 aaccatacat acccaccagc gtcagaatca gacccggaga tggcagcatc agcaccgtac
    32521 tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac
    32581 gcagcacctt atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat
    32641 ccattgagtc actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa
    32701 gggcacccag aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga
    32761 ttatcaggtc attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg
    32821 gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    32881 accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg
    32941 aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc
    33001 tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca
    33061 ccatgaattc cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct
    33121 aaagaaaaat aggtccattt ttatcgctaa aagataaatc cacacagttt gtgttgtttt
    33181 gtgcaaaagt ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga
    33241 aggatgagct ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac
    33301 gtcgcttgcc gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc
    33361 gatatgaata acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc
    33421 agctttctca aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca
    33481 ccaaagattg cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt
    33541 ctggaatgta cctgctgtac cgaatctttt atccgctccg ctcacccact gacgaaggac
    33601 gtcatccttt ccctgatttc cctctattac gacgataccc tgatggctgc cgccggaacc
    33661 caggcggaag aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca
    33721 gtagaaggta atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg
    33781 tttattgaga aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc
    33841 tgcgcgtcct ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc
    33901 gacaaagtca tcaccgacaa acccattatc aaagtaccta gctgcccgcc gatcccggat
    33961 gtgatgagcg ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga
    34021 atgggtcgtc cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct
    34081 cacttcgacg ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat
    34141 tgcctgtaca aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc
    34201 tggaatgatg gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa
    34261 aatggtttct gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt
    34321 actcattcca ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt
    34381 ggtgtgcacg cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca
    34441 gaaaccgaac atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc
    34501 agggatacac catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg
    34561 aaggccacat gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct
    34621 cctgcggcac catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg
    34681 cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg
    34741 tttacgccat cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca
    34801 acattatgct gacaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg
    34861 ggatggactg gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac
    34921 tggcgcaaag tctctcctct tggccgaaat catcccctag ctatttcttc gacgtacaaa
    34981 atcgcctgaa gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg
    35041 ggcacccgca gtacaaattg ccgctagaag ccaacctgat gggctttgcc cactatctcg
    35101 aagctctcga tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc
    35161 cgcatccaaa ctggattgtc ggcgggatgc cttgcgccat taacattgac gaaagcggcg
    35221 cggtcggggc ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg
    35281 cagacttcat taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac
    35341 cgtggagcga aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc
    35401 cggatattgc caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta
    35461 acggcgactt caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt
    35521 ttgtcgatca cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg
    35581 gcattaccga cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc
    35641 agctgaatga acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga
    35701 tggaagtggg gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg
    35761 ttgagtcggt cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa
    35821 cgttgggccg caggtaagtt gcagtatttc ttcgacaagc tgatgaccaa cctgaaaaat
    35881 ggcaatctcg ccactgcttc cactgaaaaa tgggaacctg caacctggcc gacagagtgc
    35941 cgtggcgtcg gctttaccga agcgccgcgc ggagcgttag gccactgggc cgccattcgc
    36001 gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca cctggaacgc cagcccgcgc
    36061 gatcccaaag ggcagattgg cgcttatgaa gcggcgctga tgaacaccaa aatggcgatc
    36121 cccgagcaac cgctggagat cctgcgtact ctgcacagct ttgatccgtg cctcgcctgt
    36181 tcaactcatg tgctgggcga cgacggtagc gagctgatct ccgtgcaggt gcgttaacag
    36241 cgaaggagaa tcatcatgca acagaaaagc gacaacattg tcagccacta tgtctttgaa
    36301 gcaccagttc gtatctggca ctggctgacg gtgttatgca tggcggtgtt gatggtcacc
    36361 ggttacttta tcggcaagcc gctaccttcc gtcagtggcg aggcgacgta tctgttctat
    36421 atgggttaca tcaggttaat tcacttcagc gccgggatga tttttaccgt ggtcttgctg
    36481 atgcggatct actgggcttt tgttggcaat cgttactccc gcgagctgtt tatcgtgccg
    36541 gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa tccgctggta tctgtttctg
    36601 gcaaaacgtc cgagtgccga tataggccat aaccccatcg cccaggcggc gatgttcggc
    36661 tatttcctga tgtcggtctt tatgatcatc accggttttg cgctgtacag cgaacacagc
    36721 cagtacgcta tttttgcgcc gttccgttat gtggtggaat ttttctactg gacgggtggc
    36781 aactcaatgg acattcacag ctggcatcgg ctggggatgt ggctgattgg ggcgtttgtg
    36841 atcggtcatg tctacatggc gctgcgtgaa gacatcatgt ccgacgacac ggtgatctcc
    36901 accatggtca acggctaccg tagccacaaa tttggcaaaa taagtaacaa ggagcgttca
    36961 tgagcgagca acgcgtggtg gtcatggggc tgggcaacct gctgtgggcc gatgaaggct
    37021 tcggcgtgcg ggtggcggaa cggctgtatg cccattacca ctggcccgag tatgtggaga
    37081 ttgtcgatgg cggtactcag ggactgaact tgctggggta tgtcgaaagc gccagccatc
    37141 tgttgattct cgatgccatt gactacgggc tggaacctgg aaggctgcga acctatgccg
    37201 gagaacgcat tccggcctat ctcagcgcga agaaaatgag cctgcatcag aacagtttct
    37261 ccgaagtgct ggcgctggca gatatccgcg gacatctgcc agcacatatt gccctcgtcg
    37321 ggctgcaacc cgcgatgctc gacgactacg gcggcagcct gagcgaactg gcacgggagc
    37381 aactgcccgc tgcggaacaa tcggcgctgg cgcagttggc tgcgtgggga agtgtgccgc
    37441 aaccggctaa tgaatcgcgt tgtctcaatt atgactgtct gtcgatggaa aattacgaag
    37501 gcgttcgctt gcgccagtac cggatgacac aggaggagca gggatgagca acgacacgcc
    37561 atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg acgccggtca gtgaatcccg
    37621 tcttgacgac tggcttacgc aagcgccaga cggcgtggtg ttattaagca gtgacccgaa
    37681 acgcacgcca gaggtcagcg ataatccggt aatgattggc gaattactgc acgagtttcc
    37741 cgactataca tggcaggtgg cgattgctga ccttgagcag agcgaagcca tcggcgatcg
    37801 ctttggcgcc tttcgctttc ctgccacttt agtgtttacc ggcggaaact atcgcggcgt
    37861 gctgaatggt attcacccgt gggcggaact gataaacctg atgcgcgggc ttgtcgaacc
    37921 gcagcaggag cgtgcctcat gagcgaaact tttttccatc tgctggggcc aggaacgcaa
    37981 ccgaacgatg acagtttcag catgaatcca ctgccgatca cctgtcaggt gaatgatgaa
    38041 ccgagtatgg cggccttgga gcaatgtgct cacagcccgc aggtgattgc gctgttaaac
    38101 gagttacaac atcaactaag cgaacgccag ccgccgttgg gcgaggtgct ggcagtcgat
    38161 ctgttaaatc tcaacgccga cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa
    38221 gtgtcagtgc gcattcagca ggctgacgac agtaaaagtg aaatacagga ggcgatcttc
    38281 tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt tgctggagga caaactggag
    38341 gctggctgcg cgccgctggc gttgtggcag gcggcaacgc aaaatctctt gccgacagat
    38401 tcgctgttac cgccgcccat tgatggcctg atgaatggcc taccgttggc gcatgagtta
    38461 ctggcacatg tacgtaaccc cgacgcgcag ccgcacagca ttaatctgac gcaattaccc
    38521 atcagcgagg ctgatcggct ttttctctca cgtctctgtg ggccgggaaa tattcagatt
    38581 cgtaccattg gctatggcga gagctatatc aactccacgg ggttacgcca tgtctggcat
    38641 ttacgctgta cggacacctt aaaaggcccg ttactggaaa gttatgaaat ctgcccaata
    38701 ccggaagtgg tgctggcagc gccagaagat ttggtcgact ctgcgcagcg gcttagcgag
    38761 gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag acggtaagta tcgctttcag
    38821 tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag agtttcagga taaagaggga
    38881 gatctaacat tatcgggtta tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt
    38941 cattgattta tcgcgctggc agtttgctct gaccgcgctg tatcactttt tatttgtacc
    39001 ccttaccctg gggctgattt ttttgctggc tatcatggaa accatttacg tggtcaccgg
    39061 caaaacaatc taccgcgata tgacgcgctt ctggggtaag ctcttcggta tcaattttgc
    39121 tcttggcgtg gctaccggcc tgaccatgga gtttcagttt ggtactaact ggtcattcta
    39181 ttccaactat gtgggcgata tttttggcgc accactggcg atggaagcat taatggcctt
    39241 cttcctcgaa tccacctttg tcgggctgtt cttcttcggc tggcaacggc tgaataaata
    39301 ccagcacctg ctggtgacgt ggctggtggc gttcggttca aatctctctg cattgtggat
    39361 attgaatgcc aacggttgga tgcaataccc gaccggtgcg cattttgata tcgacaccct
    39421 gcgtatggag atgaccagct tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt
    39481 tgtgcacacc gtcatggcgg gctacgtgac cggggccatg tttattatgg cgatcagcgc
    39541 ctggtattta ctgcgcggac gggagcgcga tgtcgcatta cgctcgtttg ccatcggttc
    39601 cgtcttcggt actctggcga ttatcggtac cctgcaactc ggagacagtt ctgcgtatga
    39661 agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa ggggagtggc aaacggaacc
    39721 tgctcctgca ccgttccatg tggttgcctg gccggaacag gatcaagagc gtaacgcctt
    39781 tgccatcaaa attcccgcgc tgctagggat cctcgccact cactcattag ataaacccgt
    39841 gccgggtctg aagaatttga tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc
    39901 ctggctgtta atgcaggaaa tatctcaagg caatcgtgag ccgcatgtgt tgcaggcatt
    39961 ccgggagctg gaaggcgacc ttggttacgg catgttgctc tcccgctatg cgccggatat
    40021 gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc
    40081 gccggtattc tggagtttcc gcatcatggt gggctgtggt tccctgctgc tactggtgat
    40141 gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa
    40201 aatggcgctc tggagtttgc cgttgccgtg gattgcgatt gaagccgggt ggtttatgac
    40261 cgagtttggt cgtcagccgt gggcgataca ggacatctta ccgacatact ccgcgcactc
    40321 cgctttaacc acaggacaac tggctttctc actgatcatg atcgtaggtc tttacaccct
    40381 gttcttaatc gccgaagtct acctgatgca gaaatatgcc cgtctggggc cgagcgcgat
    40441 gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa
    40501 acattgcgct tcatctggtg gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc
    40561 gacggatttg acatggggat cggctgtttg ctgccgctgg tggcgcgtaa tgatgatgaa
    40621 cgccggatag tgattaatag cgttggtgcg cactgggaag gcaatcaggt ctggttgatc
    40681 ctcgcaggtg gggcattatt tgccgcctgg cccagagtgt atgcagcggc gttttccggc
    40741 ttttatgtgg cgatgatcct ggtgctgtgc tcgctgttct tccgtccgct ggcctttgat
    40801 tatcgcggaa aaattgccga tgcacgctgg cgtaaaatgt gggatgccgg tctggtcatc
    40861 ggcagtctgg tgccgccggt agtcttcggt atcgccttcg gcaacttgtt gctcggcgtg
    40921 ccgtttgcct tcacaccgca attacgcgtg gagtatctcg gcagcttctg gcaactgctg
    40981 acgccattcc ctttattgtg tggtttgctc agccttggga tggtgatttt gcaaggtggc
    41041 gtctggttac aactgaaaac tgttggtgtg attcatctgc gttcacagct ggcgactaaa
    41101 cgcgctgcac tgttggtgat gctgtgcttt ttgctggcag gttactggct gtgggtcggt
    41161 attgatggct ttgtactgct cgcccaggat gctaacggtc cttccaatcc gttaatgaaa
    41221 ctggtggcag tgctacctgg tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg
    41281 atcttcccgc tgctggggtt cttctgccca ttgctgacgg tgatggcgat ttatcgtggt
    41341 cgcccgggtt ggggattttt gatggcatca ttgatgcaat ttggcgtgat tttcacggca
    41401 ggcatcacgc tgttcccctt tgtcatgccg tcaagtgtga gtccaatctc cagcctgacg
    41461 ttgtgggaca gcacttccag tcagctgacg ctgagcatta tgttggtaat cgtgctgata
    41521 tttttgccca ttgtgttgct ctacactctc tggagctact acaaaatgtg gggacgcatg
    41581 acaacagaaa ctctccgccg taacgaaaac gagttgtact aaggagcagg aacatgtggt
    41641 atttactttg gttcgtcggc attttgttga tgtgttcgct ctccaccctt gtgttggtat
    41701 ggctggacct gcgtctgaaa agttaacgaa cgtaggcctg atgcggcgca ttagcatcgc
    41761 atcaggcaat caataatgtt agatatgaaa agcggaaaca tatcgatgaa agcgatctta
    41821 atcccatttt tatctcttct gattccgtta accccgcaat ctgcattcgc tcagagtgag
    41881 ccggagctga agctggaaag tatggtgatt gtcagtcgtc atggtgtgcg tgctccaacc
    41941 aaggccacgc aactgatgca ggatgtcacc ccagacgcat ggccaacttg gccggtaaaa
    42001 ctgggttggc tgacaccgcg cggtggtgag ctaatcgcct atctcggaca ttaccaacgc
    42061 cagcgtctgg tagccgacgg attgctggcg aaaaagggct gcccgcagtc tgctcaggtc
    42121 gcgattattg ctgatgtcga cgagcgtacc cgtaaaacag gcgaagcctt cgccgccggg
    42181 ctggcacctg actgtgcaat aaccgtacat acccaggcag atacgtccag tcccgatccg
    42241 ttatttaatc ctctaaaaac tggcgtttgc caactggata acgcgaacgt gactgacgcg
    42301 atcctttgca gggcaggagg gtcaattgct gactttaccg ggcatcggca aacggcgttt
    42361 cgcgaactgg aacgggtgct taatttcccg caatcaaact tgtgccttaa ccgtgagaaa
    42421 caggacgaaa gctgttcatt aacgcaggca ttaccatcgg aactcaaggt gagcgccgac
    42481 aatgtctcat taaccggtgc ggtaagcctc gcatcaatgc tgacggagat atttctcctg
    42541 caacaggcac aaggaatgcc ggagccgggg tggggaagga tcaccgattc acaccagtgg
    42601 aacaccttgc taagtttgca taacgcgcaa ttttatttac tacaacgcac gccggaggtt
    42661 gcccgcagtc gcgccacccc gttattggat ttgatcatgg cagcgttgac gccccatcca
    42721 ccgcaaaaac aggcgtatgg tgtgacatta cccacttcag tgctgtttat tgccggacac
    42781 gatactaatc tggcaaatct cggcggcgca ctggagctca actggacgct tccaggtcag
    42841 ccggataaca cgccgccagg tggtgaactg gtgtttgaac gctggcgtcg gctaagcgat
    42901 aacagccagt ggattcaggt ttcgctggtc ttccagactt tacagcagat gcgtgataaa
    42961 acgccgctgt cattaaatac gccgcccgga gaggtgaaac tgaccctggc aggatgtgaa
    43021 gagcgaaatg cgcagggcat gtgttcgttg gcaggtttta cgcaaatcgt gaatgaagca
    43081 cgcataccgg cgtgcagttt gtaatgcata aaaaagagca ttcagttacc tgaatgctct
    43141 gaggctgatg acaaacgaag aactgtctaa tg
//
LOCUS       DAJTYI010000017        43132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYI010000017 DAJTYI010000000
VERSION     DAJTYI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(930..2612)
                     /gene="glcA"
                     /locus_tag="OO564_000941"
     CDS             complement(930..2612)
                     /gene="glcA"
                     /locus_tag="OO564_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR6652163.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
     gene            complement(2967..5138)
                     /gene="glcB"
                     /locus_tag="OO564_000942"
     CDS             complement(2967..5138)
                     /gene="glcB"
                     /locus_tag="OO564_000942"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6652164.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5160..5564)
                     /locus_tag="OO564_000943"
     CDS             complement(5160..5564)
                     /locus_tag="OO564_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR6652165.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5569..6792)
                     /gene="glcF"
                     /locus_tag="OO564_000944"
     CDS             complement(5569..6792)
                     /gene="glcF"
                     /locus_tag="OO564_000944"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR6652166.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6803..7855)
                     /gene="glcE"
                     /locus_tag="OO564_000945"
     CDS             complement(6803..7855)
                     /gene="glcE"
                     /locus_tag="OO564_000945"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6652167.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7855..9354)
                     /gene="glcD"
                     /locus_tag="OO564_000946"
     CDS             complement(7855..9354)
                     /gene="glcD"
                     /locus_tag="OO564_000946"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR6652168.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9600..10370
                     /gene="glcC"
                     /locus_tag="OO564_000947"
     CDS             9600..10370
                     /gene="glcC"
                     /locus_tag="OO564_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6652169.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10494..11636)
                     /locus_tag="OO564_000948"
                     /pseudo
     CDS             complement(10494..11636)
                     /locus_tag="OO564_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12040..13728
                     /locus_tag="OO564_000949"
                     /pseudo
     CDS             12040..13728
                     /locus_tag="OO564_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            13725..14639
                     /locus_tag="OO564_000950"
     CDS             13725..14639
                     /locus_tag="OO564_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6652170.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14672..14920
                     /locus_tag="OO564_000951"
     CDS             14672..14920
                     /locus_tag="OO564_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6652171.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            14920..16092
                     /locus_tag="OO564_000952"
     CDS             14920..16092
                     /locus_tag="OO564_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6652172.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16128..17207)
                     /gene="lptG"
                     /locus_tag="OO564_000953"
     CDS             complement(16128..17207)
                     /gene="lptG"
                     /locus_tag="OO564_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6652173.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17204..18274)
                     /gene="lptF"
                     /locus_tag="OO564_000954"
     CDS             complement(17204..18274)
                     /gene="lptF"
                     /locus_tag="OO564_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6652174.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18305..18865)
                     /locus_tag="OO564_000955"
     CDS             complement(18305..18865)
                     /locus_tag="OO564_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6652175.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(18877..19701)
                     /locus_tag="OO564_000956"
     CDS             complement(18877..19701)
                     /locus_tag="OO564_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652176.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19701..21050)
                     /locus_tag="OO564_000957"
     CDS             complement(19701..21050)
                     /locus_tag="OO564_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6652177.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSVWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21096..21854)
                     /locus_tag="OO564_000958"
     CDS             complement(21096..21854)
                     /locus_tag="OO564_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652178.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21886..22599)
                     /locus_tag="OO564_000959"
     CDS             complement(21886..22599)
                     /locus_tag="OO564_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6652179.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22773..23465
                     /locus_tag="OO564_000960"
     CDS             22773..23465
                     /locus_tag="OO564_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6652180.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23514..25013)
                     /gene="pitB"
                     /locus_tag="OO564_000961"
     CDS             complement(23514..25013)
                     /gene="pitB"
                     /locus_tag="OO564_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6652181.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25305..27164)
                     /gene="gss"
                     /locus_tag="OO564_000962"
     CDS             complement(25305..27164)
                     /gene="gss"
                     /locus_tag="OO564_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6652182.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27369..28235
                     /gene="yghU"
                     /locus_tag="OO564_000963"
     CDS             27369..28235
                     /gene="yghU"
                     /locus_tag="OO564_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6652183.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28358..28606)
                     /gene="hybG"
                     /locus_tag="OO564_000964"
     CDS             complement(28358..28606)
                     /gene="hybG"
                     /locus_tag="OO564_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6652184.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28740..29081)
                     /gene="hypA"
                     /locus_tag="OO564_000965"
     CDS             complement(28740..29081)
                     /gene="hypA"
                     /locus_tag="OO564_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6652185.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29074..29562)
                     /gene="hybE"
                     /locus_tag="OO564_000966"
     CDS             complement(29074..29562)
                     /gene="hybE"
                     /locus_tag="OO564_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6652186.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29555..30049)
                     /locus_tag="OO564_000967"
     CDS             complement(29555..30049)
                     /locus_tag="OO564_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6652187.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30049..31752)
                     /gene="hybC"
                     /locus_tag="OO564_000968"
     CDS             complement(30049..31752)
                     /gene="hybC"
                     /locus_tag="OO564_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6652188.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31749..32927)
                     /gene="hybB"
                     /locus_tag="OO564_000969"
     CDS             complement(31749..32927)
                     /gene="hybB"
                     /locus_tag="OO564_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6652189.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32917..33903)
                     /gene="hybA"
                     /locus_tag="OO564_000970"
     CDS             complement(32917..33903)
                     /gene="hybA"
                     /locus_tag="OO564_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6652190.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33906..35024)
                     /gene="hybO"
                     /locus_tag="OO564_000971"
     CDS             complement(33906..35024)
                     /gene="hybO"
                     /locus_tag="OO564_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6652191.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35213..35500)
                     /locus_tag="OO564_000972"
     CDS             complement(35213..35500)
                     /locus_tag="OO564_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6652192.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35619..36506)
                     /locus_tag="OO564_000973"
     CDS             complement(35619..36506)
                     /locus_tag="OO564_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6652193.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36663..37703
                     /gene="gpr"
                     /locus_tag="OO564_000974"
     CDS             36663..37703
                     /gene="gpr"
                     /locus_tag="OO564_000974"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6652194.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37743..38237)
                     /locus_tag="OO564_000975"
     CDS             complement(37743..38237)
                     /locus_tag="OO564_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6652195.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38428..39313
                     /gene="yghA"
                     /locus_tag="OO564_000976"
                     /pseudo
     CDS             38428..39313
                     /gene="yghA"
                     /locus_tag="OO564_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(39585..40010)
                     /gene="exbD"
                     /locus_tag="OO564_000977"
     CDS             complement(39585..40010)
                     /gene="exbD"
                     /locus_tag="OO564_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6652196.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40017..40751)
                     /gene="exbB"
                     /locus_tag="OO564_000978"
     CDS             complement(40017..40751)
                     /gene="exbB"
                     /locus_tag="OO564_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6652197.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41003..42190
                     /gene="metC"
                     /locus_tag="OO564_000979"
     CDS             41003..42190
                     /gene="metC"
                     /locus_tag="OO564_000979"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6652198.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42330..42989
                     /locus_tag="OO564_000980"
     CDS             42330..42989
                     /locus_tag="OO564_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6652199.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43029..>43132)
                     /locus_tag="OO564_000981"
     CDS             complement(43029..>43132)
                     /locus_tag="OO564_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6652200.1"
                     /translation="GYESASQFSREFKRYFGVTPGEDAARMRAMQGN"
BASE COUNT        10682 a        11518 c        10629 g        10303 t
ORIGIN      
        1 caaaatgtca tcacttgcag agttatcgct gacagatacc tttaatctct tactactttc
       61 gtgttattgc aatacaacca tccgtaaagc agggctattt ttagcaggaa atactcaggt
      121 gtcaacattc gaactccaac tctgaaatat tcatataaat taatgatttt aatgattttt
      181 tcatttcttc gcgcctaata aaagtattca aattcagatg atttttctgc tcaacagaca
      241 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      301 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      361 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      421 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      481 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      541 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      601 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      661 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      721 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      781 gaagccaagt tgtataaata ttaaacaatt cgcatcgtga aacggagact acgtataaag
      841 aagccaatcg taaaaacggt tccctggcga gataaacggc agacaaatgg aaagccgggt
      901 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      961 gcctgcagca gggtgataac gccgataacg ccggcaaaaa tcagactgtg cttcacggtg
     1021 tagcggaaca gttcagattc tcggcccacc atgcccgtcg cggcgcaggc cacggcgata
     1081 gattgcgggg agatcatctt gccagttacg ccgccgctgg tatttgctgc caccagcaga
     1141 gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca gtgaaccaaa cagggcgttt
     1201 gaagaagtgt ccgacccggt aaggaatacg cccagccagc cgagaaacgg tgagaaaaac
     1261 gggaacatca cgcctgtacc tgccagtacc agcgccagcg tggtggacat gccggaatag
     1321 ttggtgacga aggcgaatgc cagcaccatg ccaatcgaca atatcggcca cttcagacta
     1381 attagcgttt cggcaaagac gccaatccct ttcttgatgc ccacgccgag gatgaagata
     1441 gagataatcg ccgcaataaa aatagcggtg ccgccagccg agagaggttc gaatttaaac
     1501 accgcatcca ttggcgttgg ttgggcgaca atgggtgccg ctttcaacac ttgttgatgc
     1561 aaatgaggga tctggaaatt aatcaccagt gaataaaacg cgccgcccgg agcaaataac
     1621 gctttaaacg gcttcatggt ccagatggtg accagcaccg ttaagattaa aaacggtgac
     1681 cacgcacgaa tgatttgccc cagactatat tctgaaggca cgggaccgcc agaagatggc
     1741 ttatttacca ccatcgcacc tgcggattgc cccatgctga ttgccgtttc ggtatttttc
     1801 ggccgccaga ctttaaggaa taaagcgagt gagacgatac tcaccagcgc cgaagtaata
     1861 tccggcagtt ccggaccaat atagttagag gtaaagaact gagtgacagc gaagcttccc
     1921 ccagcaacca gcgccgctgg ccacgtctct ttcacccctt tccagccgtc catcattgct
     1981 accagccaga acggcacaag aaccgacagg aacggtaact gacgtcccgc cattgcgcca
     2041 atgtggaacg gatcgattcc cgttacctga ccggcgacca gaatcggcac gcccaacgca
     2101 ccaaacgcca ccggcgcagt attggcaatc agacacagcc ccgccgcgta taacggtttg
     2161 aagcccaggc ccaccagcag cgcaccggta atcgccaccg gcgcaccaaa gccagccgct
     2221 ccttccagca acgcaccaaa ggagaaacca atcagtaaca cctgcaaacg ctgatcgtcg
     2281 gtgatggaga taaccgagct gcggataata tcgaactgcc cgctggcaac ggttaattta
     2341 tacaggaaca ccgccgcgac aataatccac gcaattggcc ataatccata aataaagcca
     2401 tagcccgcag cagcaaatgc catatcaatc ggcattttaa aggcgaatat cgcaatcagg
     2461 atagataata taagggttat tgctccagcg acatgtcctt tcagacgtaa taccgcgagt
     2521 gcaacgaaga agaatattat cgggatcagg gcgaccagag cggatagccc cagtcctccc
     2581 atcggcatat acatttgggt ccaggtaacc atattgttca gtctcttatt atctttatat
     2641 gcttgatata cttaaggttg taataagcaa aagaggactg aactgtaaaa tataggcgtt
     2701 atactttaca gcaacagtac gccgctaacg caattgctac ctctggcata acaagtatat
     2761 cgggtaaggg tttctgttcc gcacacgcag gcgcagagta tcgttaagat gtccatattg
     2821 ttgttttagg cccgctagta atgcgctacg ggtatttaat attgttaaac cctgataatc
     2881 gctccggtta tttccgggat aaatgtacta ccgcagttac tatcatagcc ccgacaataa
     2941 aacttgccgg ggcttttttg acgctattaa tgactttctt tttcgcgtaa acgccaggcg
     3001 tgtaataacg gttcggtata accgtttggc tgtttcacgc cgaggaagat taaatcgctg
     3061 gcagctttaa aagcacacga gttagcgaaa ttccccgcca tcggacgata agccggatcg
     3121 ccagcatttt gctgatcaac cactttcgcc atattctcca gcgacgcctg cacctgttct
     3181 ttggtcagaa taccgtgacg taaccagttg gcgatatgct ggctggagat acgcagcgtt
     3241 gcgcggtctt ccatcagcgc cacgttgtga atatccggca cttttgaaca accaatcccc
     3301 tgctccaccc aacgcaccac gtaccccaga atcccctgca cgttgttatc cagctcttgt
     3361 tggatctctt gcgccgacca gttagcgttt tcagccaccg gaatagtcag cagatcgtcc
     3421 agcagcggtt caaattcagc attgaactcg gtctgggcaa tgttggcttg cacgctctgt
     3481 acgttggttt ggtggtagtg cagcgcatgg agcgtagcag cggttggtga cggaacccag
     3541 gctgtgtttg ccccggcacg cagttggtcg cccttctggc tgtacatgtc tgccatcagg
     3601 tccggcattg cccacatgcc tttaccgatt tgcgctttac cgcgcagccc acagaacaga
     3661 ccggaaagca cgttattacg ttcgtaggct ttgatccacg gcgtcgattt catctggttt
     3721 ttacgcagca tcgggccagc ttccatcacc gaatgcattt catcgccggt acggtcgagg
     3781 aaaccggtat tgatgaacgc cacgcggttg cgcgcctgag cgatacagct ccgcaagttc
     3841 agcgaggtcc ggcgttcttc atccataatg cccattttca gggtattcgg tactataccg
     3901 agcattgtct caatgcgggt aaacagtttg ttggcgaacg ccacttcctg cggaccgtgc
     3961 attttcggtt tcacaatata gacgctgcca gtgcgcgagt ttttctgcac ttttaaatca
     4021 tagagggcaa tcgcgccagt catgacgcca tcaagaatac cttccgggat ttcattgcct
     4081 tcgctgtccc aaatcacagg aatggtcatc aaatgaccca cgttgcggat aaacagcaac
     4141 gagcgtccgt gcagagaaat ttcagagcca tcggcggcgg tgtactgacg atcgtcattc
     4201 agtttacgta cgatttgccg accgtttttc tccattttct cttgcagagt cccctgcatc
     4261 aggcccagca gattgcggta cagcaggatt ttatcttccg catcaaccgc cgcgaccgaa
     4321 tcttcgcagt cgagaatggt actgatagca gcttcgacga taacatcgtt gatgtgcgcc
     4381 ggatcgtctt tgccaatccg cccgttggca tcaatttgca gttcaatatg caggccgtta
     4441 tttttcagca aaatgcaggt cggcgcagcg gcatcgccac ggtaaccgac aaactgtgct
     4501 ggagtacgta acgtggtttc tttaccattt ttcaactgga tgcgtaattg tttatcgacc
     4561 accttaaacg ccaccacatc ctgatagctg ccgttttcca gcggtagtga ttcatcgagg
     4621 aaacgtcgaa cccaggcgat aacctgctca ccgcgctgcg gatcgtagcc actgaccatc
     4681 gccccttcct gcgggatgat gtcgctgccg tataacgcat cgtacagtga gccccagcga
     4741 gcgttcgccg cgttcagcgc gtagcgggcg ttcattgccg gaaccaccag ctgtggcccc
     4801 gcctgactgg tgatttcgct gtcaatgccc gtggtttcca ccgtcacgcg ctccggttgc
     4861 ggcaccaggt agcccagttc acgaaggaaa gatttatagg ccgctttatc ttttaccggc
     4921 cccggattgc tgcgatgcca ctcatcaagc gccgcctgaa tgcgatcgcg ttctgccagc
     4981 aactgacgat tttctggtgc cagatcgtga acgatctcat caacattgtg ccagaatgca
     5041 gcagcgtcca gttccacgcc cggcaaaact tctccatcca caaaacgttt aaaattggcg
     5101 tcaatgcgta aacggccctg ggttatggtt tgactcattg tttctctcct cgttttcgct
     5161 tatttcgcca acaccgctgc tgccgctttc gcgacctgcg catcctgtgc tccggttaaa
     5221 ccagaaacgc ccacggcacc aataatttgc ccatcaacaa caaccggtac gccgccttcc
     5281 agcgacgtca gtaacggcgc agtcacgaac gcggtacgtc cgttgttcac catctcttca
     5341 tagcccttag tttcacgacg ccccagcgcg gcggtacgcg ctttctcctg ggagatgtaa
     5401 gccgcaatcg gcgcgcaatc gtccatgcga cttaacgcca gcagatgacc gccgtcatcg
     5461 gcaacagcaa tggaaacaga ccagttattt ttctgcgcct cttcctgacc tgcggcaata
     5521 attgcactcg ccatttgctg gctaagaatg actttagttt tcattttttt attccttttc
     5581 aagggcttgt tctacaattt caatccagtg acgcacagag gtacgaccgg cgctcgccag
     5641 atgcgtctgg caaccaatgt tggcggtgac gatcatttcc ggtttgccgc tttccagcgc
     5701 attcattttg ttatcccgca gctggcgtgc cagatcggga tgcgttaacg catatgttcc
     5761 cgctgaaccg cagcacagat ggctgtcggg aacgtccgtt aaggtaaatc caagacgaag
     5821 taacacgttt tccacttcgc cgttcagctt ttgcgcatgt tgtagggtac acgggcagtg
     5881 gaaggccagt tttttatcgc cgcgaattgc cagtttttcc agcggttcct cgcgcagaag
     5941 ttcgactaaa tcgaccgcca gttcactgac ctgacgcgct ttatcggcat ataacgcatc
     6001 gtttttcagc atctgcccat actctttgac gaacgcgccg caaccgctgg cggtttgcaa
     6061 aattgcctcg gcacctgctt caatcgcggg ccaccaggca tcaatattat tgcgcgcccg
     6121 tgccaaccct ttctcctgcg cattaagatg atagtccacc gcgccacaac agcctgcttc
     6181 gttagctgac atgacgctga tccccagacg atccagcact cgcgcagttg ccgcgttggt
     6241 gttgggcgaa agcgtaggct gggcgcagcc ttccagcatt aaaacccgac gcttatggcg
     6301 cagcggcgga cgcggtttag ctttcaccgt ttcagcaggc agttttgctc tgacctgttc
     6361 cggtaaaaac ggtcgcagca ccagccctac ctgcgtcagc gcacggaaga ccgccggacg
     6421 cggcactacc tggcgcaatc cttcgcgcag cattcgctcc ggcagtgggc gtttcacttt
     6481 ctgctcgaca atatcacgcc cgatatccag caaattgtga tagcgcacac cagaaggaca
     6541 ggtggtttca caattgcggc aagtgaggca gcgatcgaga tgctcctgtg ttttaagcgt
     6601 gacttcgttg ccttccagca cctgtttaat cagatagatg cgcccgcgcg gcccgtccag
     6661 ttcatcgccc agaagctgat aggttgggca ggttgcggta caaaatccgc agtgaacaca
     6721 ggcgcgcagg atgctgtcgg cttccagcgc gcgcgcgttc tgccgcatct cttcggttaa
     6781 tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat gcgaccgggg ttaaacacgc
     6841 cgcaagggtc gagctgctgt ttaagctgct ggtgatagca gaataaagga gccgatagcg
     6901 gggcaaagcc accatctccg gcactaaagc gggtcgcatg accgccagcg ttgcgggcga
     6961 tgcgatggat ttgattgtca tcggctgtcg atttcagcca gcgtaacgcc ccgccccagt
     7021 cgatcagttg ctcgccgggt aaatccatca tcggcgcatc gctgggtaat gaaatgcgcc
     7081 ataaggtacc tggtaacgag aagaacggca gttgttgttc acgcaattgc tgccagaact
     7141 gacccgcaac ctcttcgcca cccagcagtt cacgcgctgc ttttaccgat ccttcgccgc
     7201 cctcaaggcg gatccacaac gcattgtcga agtaacataa gccactaatg ggtaatggct
     7261 ggagttgcca ctcggcgatt tcactcatgg cttcttgcag gctgatttcc cgacgcaggc
     7321 tcagagaggc acgcggtcgc ggtaacactt tcattgagat ttcagtgagc acgccaagac
     7381 aaccgtagct tccggccatt aaccgtgaga gatcgtatcc ggcaacgttt ttcatcactt
     7441 cgccaccaaa acgcagatgt tttccagtgc cggtaatgat gcgcgtgccg aggacaaaat
     7501 cgcggaccga accgctccac gggcgacgcg gccccgccag cccgcaggcg accatcccgc
     7561 cccaggtggc ttcttcacca taatgcggcg gctcacaggg aagcatttgc ccggcgctct
     7621 ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg agcggttatc accagctcgg
     7681 tcgggtcgta attaacaatg ccgcgatgac aacgcacatc cagcgtttgc ccggtgactg
     7741 ggcgacctaa aaaggctttg ctattgctgc cctgaatcac cagcggcgtt ttatcgctaa
     7801 ttgcctgatt cacctgctcc agcagcgcct gactgtaatc acactcgcgt agcatcaaaa
     7861 gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca tgcatggcac caaattcagc
     7921 acagcggtgt agcgtgggaa tgtttttccc agggttcagc aaaccatcgg ggtcaaacgc
     7981 cgccttgacc gcatggaagg tcgtgatttc atcgctgttg aactgggcgc acatttgatt
     8041 gattttttct cgcccgatgc catgttcccc actgatgctg ccgccaactt caacgcagag
     8101 ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat tcaccaagtt cgttggcatc
     8161 gaaaaggatt aacgggtgca tgttgccatc tccggcatga aagacgttgg caacacgtaa
     8221 atcatattgc tgcgataaac gggcaatgcc ttccagtacg ccaggcaggg cgcgacgcgg
     8281 gatggtgcca tccatgcagt agtaatccgg ggagatacgt cctaccgccg ggaacgcatt
     8341 tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc tgtgccaaac ggacgtcagt
     8401 cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag tcttcctgta cgtcagactc
     8461 cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg acgggataac cggcatgaat
     8521 aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc tccagcccgc cggggataat
     8581 gccattggcg atgatgtcac caaccgcaag tccggctttt tctaccgagt caaagctggc
     8641 taacagaacc cgcgccacgg gcggcttcgg cagcagtttt accgtcactt cggtggtcac
     8701 gccgagcata ccttccgatc cggtgaacag cgccagcagg tcaaaaccag gtgaatccag
     8761 cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt tgcacttcaa ttttcagcag
     8821 gttatgtacg gtcagaccat atttcaggca gtggacgcca ccggcatttt ccgccacatt
     8881 gccgccaatt gaacaggcga tttgtgagga aggatccggc gcgtaataga gattatgcgg
     8941 cgcaacggcc tgggagatcg ccaggttaca tacgcccggc tgcacgcgcg cgcggcgacc
     9001 aacggggtta atgtcgaggc tctctttaaa gcgcgccatc accaacaaca cacctttttc
     9061 cagcggcagc gcgccaccag aaagcccggt gcctgcacca cgggtcacca ccggtacacg
     9121 caggcgatgg cagacagcca gaatcgctgt cacctgttcc atttgcttag gcagaacaac
     9181 cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac tcgtaaggaa tgatctcctc
     9241 atcggtatgc aggatctcaa gtccagggac atgctcacgc agtgccatca gtaccgatgt
     9301 gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg tacaagatgc tcatgagtag
     9361 gcttcgcttt gttgtgtggc agctgatttt tgcgcgctgc ttctgtgaac agttattaag
     9421 cgggcttttc gttttcgtct atctctttag ctaccggtca gaccattttt tttccagctc
     9481 tgtgaccttg tcttggttaa cttaatgtta aattgatgta acataatcac ttgcgtgatg
     9541 tgcgtgtttt gcgagttaag aacagaaaaa ttggtcctac ctgtgcacga ggtccgggaa
     9601 tgaaagatga acgtcgccct atttgcgaag tggttgcaga gagtatcgaa cggttaatta
     9661 tcgacggcgt actgaaggtc ggtcagccgc ttccctcgga acgtcgactg tgtgaaaagc
     9721 tcggcttctc acgctccgca ctgcgtgaag ggctgaccgt gctgcgcggg cgcgggatta
     9781 ttgaaacggc gcagggtcgc gattctcatg tcgcacggct taatcgggtg caggacacca
     9841 gcccgctgat ccatctgttc agtacgcagc cgcgaacgct gtacgatctg ctcgacgttc
     9901 gcgcattact ggagggcgaa tcggcaaggc tggcggcaac gctgggaacg caggctgatt
     9961 ttgttgtgat aacccgctgt tatgaaaaaa tgctcgccgc cagtgagaac cacaaagaga
    10021 tttcgctgat cgaacatgcg cagttggatc acgctttcca tctcgccatt tgtcaggctt
    10081 ctcacaatca ggtgctggtg tttacgctgc aatcattgac cgatctgatg tttaattcag
    10141 tgtttgccag cgtaaataat ctctaccatc gaccacagca aaaaaagcag atcgatcgcc
    10201 agcatgcgcg gatctacaac gcggtgttgc agcggctgcc gcacgtcgct cagcgcgcag
    10261 cgcgcgatca tgtacggacc gtgaaaaaga atctccacga tatcgagctg gaaggccacc
    10321 atttgattcg ctcggcggtg ccgctggaga tgaacaaggt gggaatgtga gcagagtttt
    10381 accctatccc caaggcgagg ggaccgcccc tgcacttaac aggatctggt gttaaatctg
    10441 ttcatctata ccctctcccg aatgaccgcc cctgcgctaa acaggatctg gtattaaatt
    10501 tgcttttcat acaatcggta acgcttgtac ggctccgccc caatgcgttc cagcatgtta
    10561 ttcatgcctg tattggtttc gaggatccat tacatctcca gcgcatcgat cttccggcgg
    10621 gcaaacggat cgcgtaaggc ttcaatcaat aacagcgcaa tcaccgggcc gatgcggctg
    10681 aactgatact cgtcgcgcac gcccatcagc ggtactcgcg cagttcgcac accgctgact
    10741 ttcaaacgcc acagcaattt tgcccagccg aagggaaaga gcgatccgtt cagatcggca
    10801 atcgcctcgt tgatgttagg caagccgaca ataaacgcgc agggtgcaga atcaatctca
    10861 gcgatgtaga tcatatcgtc cggcaccaga tatttaagtt ggtcgcccat ggtcgcgaat
    10921 tcatgttcgg taaacggcac aaatccccag ttgtgctgcc agccagagtt gaaaatctca
    10981 cgcaggatct gcatctcttc ggcaaaccgc tgacgattga tgcagcgaat ggtcaccttt
    11041 ttgcgcacct gatccatcag tttttttagc gccggagaga aagtgagatc ggttcgctgc
    11101 atccaccacg ccagtaaatc aatgcctttg tgataaccca gttgttcaat atgcgcggca
    11161 taccacggtt tgccgtgtgg catcatcgca cagggtggtg tgtcaaaacc ttcaatcagt
    11221 aatccgcttt cctgattgat attcaggctg aaaggcccgc tgatcttact tgcaccttgt
    11281 gacttcaacc acgcttccgc tgcgccaaac aacgcggcaa aatcctgcgg atcatcaatg
    11341 gcgtcaatca tgccgaaatg accggtatct tcaccgtaac gctcacggtg caaggtatct
    11401 atttgcgcgg taatacgccc aactatctgc cccgcttttt ttgccaccca cgcctgccag
    11461 atgatatggt ccgtccccgg atttttcgca gacaaatgct cgttgcgttc aatgaataaa
    11521 gggggtatcc agtttggatc gtcgggataa agtgatgacg gaaaagcgat aaatgcctta
    11581 aggtcatttt tattaattac tttttcaatt ttaatcatca gcagatcgca ttccatatct
    11641 ctgtggataa caaataatta atggtgaaaa acaggttgca acagcaacga gcggcatctt
    11701 aataatcaac tcaatgaata caaacgacga ataaatagta ttaattaatg attatgataa
    11761 ggtcagaggc tacacagaaa cgcctcaatg ccttttgtcg atttaaggcg accggacaag
    11821 gcttcttctt aatatattca taaattaaat atcattcata ataaaaataa aaaactcaaa
    11881 ctcattattt aaatcaatgt tcattttcac accgatgaaa atactatctc atttatcttt
    11941 tatgcctata taatccaccg cattattgtc attttatttt tgatatccaa ttttttcgtg
    12001 gtgtatatgt ctaataaaat ctttacgcat tccctaccta tgcgctatgc cgattttcca
    12061 acgctggttg atgctttgga ctacgccgct ctgagtagag ccggaatgaa tttttatgac
    12121 agacgttgcc aacttgaaga tcaactggaa tatcagacat taaaaacgcg tgccgaagct
    12181 ggtgcgaagc ggttgttatc gctgaacctg aaaaaaggcg aacgcgtggc gctgattgcc
    12241 gaaacgagta gcgggtttgt agaggctttt tttgcctgtc agtatgctgg cttagtcgcc
    12301 ttcccgttgg cgattccaat gggcgtcggt cagcgggatt cctggagcgc caaactctag
    12361 ggtttactgg caagttgcca gcccgcagcc attatcactg gtgatgagta gttgccactg
    12421 gtcaatgccg cgacgcataa caaccccgaa ttacatgttt taagccacgc ttggtttaag
    12481 gcattaccgg aagccgatgt tgcgctccag cgtccagttc cgaacgatat cgcctacctc
    12541 cagtacacct ccggcagcac ccgttttccc cgtggcgtca ttatcaccca tcgcgaagtg
    12601 atggctaatc tacgtgctat aagccacgat ggcattaaat tacgccctag cgaccgctgc
    12661 gtctcctggc agcctttcta ccatgatatg ggactggtcg gctttctcct gccccccgtc
    12721 gccacgcaac tttcagtaga ttatttgcgc actcaggatt ttgccatgcg tcctttgcaa
    12781 tggcttaaat tgatcagtaa aaatcgcggc accgtttccg ttgcgccgcc gtttggctat
    12841 gaattgtgcc aacgccgcgt gaatgaaaaa gatctcgctg aactggatct ttcctactgg
    12901 cgcgtcgctg gtattggtgc tgagccgatc tccgcagaac aactccatca attcgctgaa
    12961 tgtttccgtc aggttaactt tgacaataaa actttcatgc cgtgctacgg actggcagaa
    13021 aatgcgctgg ctgtcagctt ctctgatgaa gcctccgggg ttgtggttaa cgaagtggat
    13081 cgcgacatcc tcgaatatca gggtaaagcc gtcgcgccgg gtgcagagac acgcgccgta
    13141 tcgactttcg tcaactgcgg caaagcgttg ccggaacatg gtattgaaat ccgcaatgaa
    13201 gcaggtatgc cggtcgcgga acgtgtggta ggccatattt gcatctccgg tcccagtctg
    13261 atgagcggtt actttggcga ccaggtttcg caagacgaga ttgccgcgac gggctggtta
    13321 gacaccggcg acctcggtta tctgctggac ggttatctgt atgtcaccgg acgcattaaa
    13381 gatctgatta ttattcgtgg ccgtaatatc tggccgcagg atattgaata tatagcggaa
    13441 caggaaccgg aaattcattc tggcgatgcg attgcttttg ttaccgccca ggaaaaaatc
    13501 attttgcaga tccagtgtcg gatcagcgac gaagaacgtc gcgggcagct tatccacgcg
    13561 ctggcggcac ggatccaaag cgaatttggc gtgaccgcgg ctatcgatct gttgccgccc
    13621 cacagtattc cccgaacgtc ctccggcaag cctgcccgtg cggaagcgaa aaaacgttat
    13681 cagaaggctt atgctgccag tcttaatgtg caggaatccc tggcatgaat caaactgtcg
    13741 cggtgacggg cgctaccggg tttatcggta aatatattat cgataacctg ctcgcccgcg
    13801 gctttcacgt tcgcgcattg acgcgtactg cccgcgctca cgtcaatgat aatctcacct
    13861 gggtgcgcgg ttcgctggaa gatactcatt cacttagcga gctggttgcc ggagccagcg
    13921 tagtggtcca ttgcgccggg caagtgcgcg ggcacaaaga agagattttc acccgctgta
    13981 acgttgacgg cagcctgcgc ctgatgcaag cagcaaaaga gagcggcttt tgccaacgtt
    14041 ttctgtttat ctcttcgctg gcggcgcgcc atcccgagct ctcctggtat gcaaaatcca
    14101 aatacgtcgc cgagcaacga ctggcagcaa tggcggacga aattacgctg ggcgtttttc
    14161 gcccgacagc cgtgtatggt cccggcgata aagagttaaa accgctgttt gactggatgc
    14221 tacgcggcct gctgccacga cttggtgcgc cagatacaca actctctttt cttcacgtca
    14281 ccgattttgc gcaagcagtg ggtcagtggt taagcgccga aactgtacag acgcaaacct
    14341 atgaattatg cgatggcgtc gccggtggct atgactggca acgcgtacga cagcttgccg
    14401 ccaaagcccg ttgtggttcc gtgcgaatgg ttgacattcc tctgccggta ctcacaggcc
    14461 ttgcggatat cagtaccgcg ttgagtcgcc tggcaggtaa agaacctatg ctgacccgct
    14521 cgaaaattcg tgaattaacc cacgccgact ggtcggcaag taataaccgt atttctgaag
    14581 atatcaattg gtttcccggg attagcctgg aacacgcatt acgcaacggg ctattttgat
    14641 tgtcttgttt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta
    14701 catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga
    14761 tctcgtcaac gatcttggcc tggagtcgat caaagtgatg gatctcttaa tgatgctgga
    14821 agacagattc gatatttcca ttcctattaa tattctgctg aatgtcaaac cccctgcgca
    14881 attaatggaa actctacttc catggctgga gaataaataa tggggctata cgataaatat
    14941 gcgcgccttg ctggcgagcg tctgcaattt agtgacaacg ggttaacacc gtttggcacc
    15001 tgtattgatg aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa agtgattctg
    15061 gccgggacca ataattatct cggtttaacc tttaatcatg atgccatcag cgaaggtcag
    15121 gccgcgttag cggcccaagg caccggcacc accggttctc gtatggcaaa cggcagttat
    15181 gcgccgcacc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc taccgctatc
    15241 gttttttcaa ccggttatac cgctaattta ggcgtcatca gcgcacttgc cgaccataac
    15301 gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggt
    15361 ggcgcggaaa ttattcgttt ccgccataac gatgctaaag atctggaacg gcgcatggtt
    15421 cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg agggcatcta cagcatgctc
    15481 ggtgacgttg cgccgctggc ggaaattgtt gatatcaaac gtcgtctcgg tggttatttg
    15541 attgtcgatg aagcgcattc gtttgtcgtg ttaggcgcaa cggggcgcgg actggctgaa
    15601 gctgtcggcg ttgaagatga tgtggatatt atcgttggta ctttcagtaa aagtctggcc
    15661 tcgattggcg gttttgctgt cggttcggaa gcgatggaag tgctgcgcta cggcagccgc
    15721 ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcgtcgtta
    15781 agaactattg ccacacaacc agagctgcgg caaaagctga tggataacgc caaccatctg
    15841 tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc ggtagtgcca
    15901 gtaataatcg gttcgaaaga ggaaggttta cgcatctggc gcgagcttat ctcgccgggc
    15961 gtgtatgtga atcttatcct gccgcctgcc gcgcctgcag gcatcacgct gctgcgctgt
    16021 agcgtaaatg ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc atttgctact
    16081 ctgaagcagt aatgtgagcc cgccgtggtg tttgcgccac ggcgggttta atgttgtctg
    16141 tcataccaat acaccaggct caacgccacc aacacaaacg ctaccggtaa accgagcgcg
    16201 gtaacaggtg cgcttaacgc aaatagcaat cccagattaa ccatgatttg atagctgatc
    16261 caggtgagta agcccacgat gacgcctaca gcgagacggc tacccatccc aggcgaacgc
    16321 ggggcactaa aggtaaacgg cacagccagc aaaatcatcg ccagggtcag gatgggttgc
    16381 cccagttttt cccacagagc aaggcggtat tcgctgctgg gttgcccggt ttcctgcaaa
    16441 taatggatgt aatgattaag ctgacggaca gaaaaagtat tccccggcat tgataactct
    16501 tcaagattca tactggtgaa ggccgattgc caggcaagat tatctaatgt ttccagcgtt
    16561 tctttgccat tcagccattt tttatgattc acaccatgca ggacccacgt tttgtcgtct
    16621 tcaatggtgg cactgcgtgc ataaatgtag gattcaagcg agagatcgtc gcgataatga
    16681 aatatctcca cgcccaccgg ctggccctgc tcgttcagcg atttcactgt cacaaattca
    16741 ttaccacgcc tggcccacag catattgccg gtaatgtcat cgtcctcccc caacgcggtg
    16801 gcggtcgatt tgatttgcaa cgcctgttgc tgcaatggcg acgccaccca ctcatctatc
    16861 gcgcctaacg aaacagtcca caatatcccg gcaaccagcg ccaccagtgc aatacggaag
    16921 atagaaaacc ccgtgctgcg aatggcggta agctcactgt ttttcgataa ctgccccagt
    16981 ccaacaatac cacccaataa cgcgataaac ggcgaaagtt cgaccagtgt gcgcggtaag
    17041 gtcattagca ccaccagcac cgcctgagtc cagcgataac cgcccgggct gacaccatcc
    17101 agttcgttaa tcaggttgaa ggtggtaaaa agcggcagca atagccctgc agcggcggca
    17161 aaaccgagaa agagatgacg gattaaatag cgactgaaaa cattcatcgg gaaaatttct
    17221 gcagcaaaga gaagtcacgt gcgaccagta tcagcaggcc cattagcatg agtccgggca
    17281 ctaaccacaa accaggaatg aggggaatcg cgccgttagc caccagtgta cggcagacgt
    17341 cgccgccata aaaaatggca ataaacagca acgtcagtgg caataacgtt gaaaatcgcc
    17401 cttgccacgg ttttacccga cttaatgaaa tggccagcag cgccatcaac aatgcggtca
    17461 ggccacggct ttgtcgccat tgcagttcgg catgatcggc aggaaaggcg gagcgcgcca
    17521 gctccgtgac cgatttcgct ttgcgtttga cgttcgggct ttgatccagc ggattcagat
    17581 gtaattgcag attacggtag atctgttcgt tgtcgtcacg cccctgatga tcgagaagat
    17641 aggcagtgcc gttgtgcaac ataacggtcg gtttctcggg tgatgggtca accacatcaa
    17701 ccgaacgggc gcggaaaata cgggttcgat tggcagtaga agtataaatc agcgcgtcag
    17761 tcaggcgatt attatcctga tcaaccgtct gcgaaaggat cattcgtccg ttatcgttag
    17821 tgttaaattt ctttgcccgc aactggcgaa catccagctc cgactgcgac tgctgctcca
    17881 gttgataaat ctgcgcataa gcccacggac gaccatacat cgacagaagg gtgacaaaaa
    17941 tacttagcgg tatcgccaga taaagaacgg ctttgtacaa gcgtcccgga ctgccccccg
    18001 ccgcagagat agcggtaatt tccgagtcgg tatacatctg ccctagcgtc acgccaactg
    18061 acacatacag accaacaggt aacaacatct ccagtgcaat cagtactttg tagaaaacga
    18121 tatccagcac aacatcaaga gctaatgtcc catttgccgc ttcggtcaga taacgctgtg
    18181 cggagtagct ggcgaaaata aagatcagaa aaccgacgat aatcagcacc agacggcgcg
    18241 taccacgcat gatgtagtgt tcaaccagct tcatctcact aacgccctca atcctttgag
    18301 ttttttattc attgctcagt ttctgcgcca acgcaatgac atgatctacc tcttccggcg
    18361 tcaggctgcc gtcctttgcc cactgaattt gcccggcttt attcagcacc agaatggttg
    18421 aactctgctc cggcaatcgc caggcaacgc gtccctgtcc attgccgtca ataacgaatt
    18481 gcgcccacgg atagcggcgt ttattttttt cgattttgcc aacaacaaaa tagccagtac
    18541 caaagatggc atcatcggtg ttcacaatgg tggtgggctg aaaacgatcc tgcggaaagt
    18601 ttgccgcttc aacagcttta attaacagtg agttcttttt cttcgccgac ttacgcccgg
    18661 cgatatactg aataatgcgg acttttcccg ctaattgtgt gctgttccac ttttggtagt
    18721 gaatttcgtc gtcgctattc agaagcagct cgccgccttc ctggatataa acaggggtaa
    18781 ccgttttgcc agtgacgaaa ttgtgtgcct gcacacccgg ggctaagagc atgataatca
    18841 acgcaataat caaacgagat gacatcattg ctccggctat agcggaaaac gcgaaatcag
    18901 cagctctaag cgctgacaaa tatcgctatc cagcgccaac ggtgcataat acgactggcg
    18961 gttttccata tcatccgcca ccatgtcgtc gtgtttatct cccatcaggc cattaatggc
    19021 ggcgaggaaa cgggaaatct gtaacgcctc tttattttca ggatcaagcg atcttaaggt
    19081 gtcgtaataa gaaacctgct ggctgatgat ttttcgcaca tcatgaaatt ccctgccggt
    19141 tagtcgcggt ttggcaataa tctctttcag ggtatgcatt tgcttttgcc gataggcaat
    19201 aaaactgctt acgctttcta actggctatg gcgtaatgaa tagttcacca acgaccagac
    19261 cggagagctt aaataaatcc gtaacaggtt gccgtaatac gacacaatat ttttcttacc
    19321 gttacgaaag ccgtcctgaa aacgccccag aaaaacggtc gttttgccga ataaaaatcc
    19381 ggcctgatgc ttctttaaat acagccgttg cgcaaaacgc aggtgcttgt agcgcgctcg
    19441 catgtattta aattgtttgc gctcatcagc agtcagctca tcgcctttgg cttgtttgcg
    19501 aactaaacgc agtaattcct gacgatcaac gccattgagc cagaattgca ggcataacgc
    19561 gtaattatca tgaatgattt cggtggtaca acttagtgca atagtcggcg gcaaactaac
    19621 ctgtgcatca acgacatcat cttcttcaat cgcaacaaat agacgctcgc tcgcctcaag
    19681 cgggaaatat tttctcatca tcaggatttc tctccggctg cgttataaag caaggaatgg
    19741 cgctgaaaaa atgcctttaa ggttggaatt aacgagagcg ccacgtccag acattgccca
    19801 ccgaagtaag caaacgctgc tcccagcaca ccaaacagat gacagaacat gaacagcaat
    19861 acgatgtagc cgattgaggc gatggtttgc gccacgagaa aagcgcgctg ttttcccgcc
    19921 atcaatagca atgactcctg cgggaagccc agcatcgaaa tcacaattgc gcccagcatc
    19981 acctgaatca ggtcatacgc ttcgagatac ttaacgccaa acaccagcga aatgagcggc
    20041 ttgccgacga tcaacaccgc gagcgccacc agaataccga ttccgcccgc cagtaaaccg
    20101 gacttcacac ctaacaacca cggtctggtg gtgcgcggat ctaaacgcat cacctctggg
    20161 taaaaacttt tacccagcaa acccgctggc gttccggcgg cgtcgaagaa tgtcatggcg
    20221 attttaaata acccagcggc agcgggtcct aacacgatcc ccaccaacac tgtgctgcac
    20281 gagttacgcg ccgaccagat ggagtgggca atgttggttg accagacaaa actccacgcg
    20341 cctttaatgt gtcgggcaga ctcaaacaga ttcaatttga aggcgttatg gatatttcta
    20401 cggcgtaatt cgcgcgcggc aaaccaccag tacatggtgc cgccaaccag attcgacacg
    20461 taccaggcaa taacaaaacc cgcaaaacca aagtcaaaat accaggctgc gacgctcccc
    20521 gctgcgcgca gaaaaggttt cgtcgcctgc tgtacagcaa tcaaatcaaa gcgatctacc
    20581 gcacgcagaa tgccggtcgg cgtggaggaa gccattgaag gaatgagcgt gcaatacagc
    20641 gccgccagcc aaaagctttg gtcatccaga cctaatgaat gggaaaggaa tggcagtaag
    20701 gcaatgccgc cgacaatcgc caccgcgccg ctgacgatat ccagcgagaa ggaaaatgag
    20761 acgacattgc gaaattgctg cggattattg ttggtcaagg ctggcgttcc gtactgaacc
    20821 accagctgcc atgtctgaaa cttaataaaa tcgctgatcg acttggcgta cgattgcaca
    20881 atcaccagta caccaaacat ggcgggcgtc atccctttac cggcacacga gagcgccaac
    20941 agccccagca aggcgctctc gacattactg gagcctaacc agacgctatt gcgaataatg
    21001 gtgcgaaacg cgccatctgc aaaccaatgt ttgatgttaa aaccagccaa ttcagcctga
    21061 ccttatcgtt gatagtaaaa aatatcccgc cagccttaag ttaaacttcg gcggtcagaa
    21121 acgatggcaa ccagagaaac cgccttctgt acctgttcca gcacttcgct gtagggcgct
    21181 ctggaatcaa tctcaagaat ttttgtgccg ttatagccaa tcgtcgacat gacgccgatt
    21241 ttgtcctgca gctcggcata ctcatggtca ggcttgcggg agatggcagt atcaatatca
    21301 atccccaaac gaatgattaa ttcaggacgg tattgcgcca ttttttggta taaactccgt
    21361 tcgcgctgcg ccagaaacat gctgattttc ccggtcgcac gttcgacgcc gatccccggt
    21421 ccgtcataat aaaagcccga aatttcagcc tgcgggaagc gatcgctgac caccagaacg
    21481 ccactttgcg ccagtcgctg aaccttgcgt agattcgcca ttcttcgcag cgagaagcag
    21541 tacataatca ccgccgccca tagcgccgga gatttggttt tcatgctttg ggttttcgag
    21601 gatttggccg ccagtcgccg ttccagccag acgccaacca acggcaatcg tttgattttg
    21661 tctccgtctt cgccggagag cagccccaga tagcggcgct cggtttgcca gtgttgttgc
    21721 agcgatttca ccaggtcggt ggtcagcgtg gatttaccgg tgccatcaca cccgaccacc
    21781 gcaatcagcc ccggaatgta gttgggctgc ggcgcggttg tactattaac agtttgtgtt
    21841 tgtagtgcat ccattcgtta aatcatcccc tatccatttt ggtgatcagg aaagcgcggc
    21901 gtgaattgcg cgtagtgacg cttgcataat ttcatcggct ggttgccgac catccagttc
    21961 aaggatcttg gcgccattga atgtcagttg cggcgtaacg gcgatttttt cctgtaacgc
    22021 tgccagttgg tggtcaggtt tacgcgcaaa cgcggtttgt tcatcaatgc caagacgaat
    22081 caacaatacg ggtaaataag acgccatcca ttggtacagc ttcaactcgc gctgccttaa
    22141 catttttatc caaccgttac cgcccgtggt ttttgccaat tgcgggccat caaagcgaaa
    22201 acccggcact tcaacctgcg ggtagcggtc ggtgatgagc agatagcctt gctggctttt
    22261 acacaacatt ttgcgaaact tgtacgcccg ccagcaggaa agcagataga taaccagcgc
    22321 agtaatattg ccaggcggtg ttgagggctt ttcgtgtaca tgtgccgctt tacttcgcag
    22381 ataacaccca caaggtgcgc caataacagg gagctgtgaa atccattcgc caattcgccc
    22441 ggacgattgc ccgagataaa tgtgttctgt tggcattctt gctgccagtt catttaccag
    22501 gcttgccgtg agggtcgatt tacctgaacc atcacatcct acaatggcaa taacacgcac
    22561 tggcgtggaa ttaattattg acatattacg ttgattcacg aaaaacccgg cagtaaataa
    22621 tgtattgaat attacgtggt ccgtgctcag aatatccgtt caaccttatt gtgacaatgg
    22681 ttaattgtga cattgacctg aattacgctt tcataaaaac atattaacca aataaatatt
    22741 tttaatggat atttaaatta aaggatatat tcatgcagtc aataacacct ccattaattg
    22801 ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctt attaccgttg
    22861 tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga aatgtcggtc
    22921 gtgcagtgac aaaattacct ttgatgggaa aatccttaca taaaacaatt gaacgaaatc
    22981 aggtgaaaac agcaaaaaaa ttgcctggac cagttccggc gctggtaatt acagcgtttg
    23041 tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc gggttaattg
    23101 ttctaaccga ccgttatcct caggaccaaa ttcctggcgc ttacgatggt acggtgttcc
    23161 cacctaacgt tgaaggtggt cgttttgtct catggctggc aagccaggaa cgtaaagcgt
    23221 ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt gaccttgaag
    23281 ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct ggcgaggaag attgccataa
    23341 cgccacagtt aacctttggt ggtgcacaac tggttgatat cgatgccaat cagcctctgg
    23401 aacaggtgtt ggttgatgca gaaaaagcga ttacggattt tatgaccgcg cgtggttatc
    23461 actagtcaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga aggttaaatc
    23521 aactgcaatg ctatccagta cagcccacca gaaagaaaaa ttgccgccgg taaagtaaac
    23581 acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc gtccaccacc
    23641 atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg agacaggcat cccaatataa
    23701 ctggcaaggc cgatagacac tgccgccgtc atttgtgccg ccatgccttg cgcatacgtc
    23761 atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca gccaatcatg
    23821 gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc acaccggagc gtactcaatg
    23881 gtgcttaaca tatcgctgcg cagtttcttc agcaggttct ggtcttcttt actgacgcct
    23941 ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca gcataatgcg gcgcagctgg
    24001 ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat tgcctggcag catcgtttta
    24061 acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt tacttgcgtg
    24121 ccatcagtcg atgctgcagg caatggaggt tccatggcaa tcaacttctg cggcagttca
    24181 ggatgctgtt gcaggtagtg ttcgaagttg gtaacggcat cgcgggtacg ggtaatttca
    24241 tagccggacg cattcatatt gacgacgaag ccagcagggg caatccccac cagtaccagc
    24301 attaccaggc cgatcccttt ctgcccgtcg ttcgcgccgt gcgaaaacgc cacgcctgca
    24361 gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc ctttttcttt
    24421 ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccact ccagaaacgg
    24481 cgcagcagga aaatcagacc acccgcaatg accaggccga cgatagggga aacaatcagc
    24541 gaggagaaaa ttttggttac ttcacgcagg tttaacgcat ccatcaccga tgagccggtt
    24601 aacagcgcgt tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg cgaactggag
    24661 gccggtaaac caaagaacca cgttcccagg ttccagataa tcgccgccag cagcatggaa
    24721 aagaccatcg ccagaccgtg agttgacccc atattcagca acaaatcggt tggtaacata
    24781 tggacaatgg cataggcaac gctaagtccg cccaataaca cgccaaaaaa gttaaaaaat
    24841 gccgccatca ccacagcaag ttgtggctgc atggcacgag tataaataac ggttgccacc
    24901 gcattcgccg tgtcatgaaa accattgatt gcttcgtaga acaacacaaa tgccagagca
    24961 agtaataaca aaagccccgt atatatatca aggccaacaa ataaatttag catatattag
    25021 attacgccat tttgaattta cgaacggacg cattatcagt gactttaacg gcatgggcaa
    25081 agtgaaatat cattttttaa cgataaaaag agtgttattt cttgtgtatt agacaataag
    25141 cattcattca aaacctttag aaataatcaa aacacaggta ttccactggt taatatattc
    25201 acatatgaaa tgaataaata tttgaatata taaatattga atattttgat taatccccgt
    25261 actaattatt cttcataatc agtacggggt tgctacaaca tgtattactt tttcaccaca
    25321 attaacggtt caatatcact ctcttttttg atgaccagtg attcatcacc gcgcaaacac
    25381 gtcccaccgt agttgccgcc aacggtgaag gtacatacct gaatgtattt accgtccact
    25441 ttcggcaaac accacagttg ctgatagatg tttttctgct cggcaaattt accgctggtt
    25501 ttgtccagca cctcttcatg atggccgacc aggtcgatat tgctaccaca gcgaccggcg
    25561 atcggtttca ctgcgtaacc tgttttcacc aattcatcat taacagtgaa atcggtatcc
    25621 agcaggtaac gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc tttgttgccg
    25681 gggatcaccg tccacagcgg ctcaaagacc agcacttccg ggcgcagcaa tacgtcgata
    25741 agacgcactt cgttttgcgg atgaccggta cggattggca ccgcagcaaa ctcacggtcg
    25801 ctaacttcac gaatctgatc aaacgcggtt tcccacgccc aggttttcca cacgcagtta
    25861 accagtcgcc cttccccatc aatcagttgc ccggcagcat cccagcccag ttcatccagc
    25921 ccgcgcaaga tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat aaactgcgcg
    25981 tgatagtttt cctcgatatc tttgtcctgc atgatatgga caaacggacg tgcacgactg
    26041 tgtttccagg caccagccag ttcgttaatc agcccttccg ccggattgaa gccgttgcct
    26101 ttatagccct gctccgccca acgttcgagg atcaagcccg cttcggtatg acaggaggcg
    26161 gagtcggcgt tgtattcgta aaccttcagg ccacgctcat ccatgcagaa atccatacga
    26221 ccagtgatca tatggtgacg gcgacgctgc caggagagac gcaaacgtgg ccagaggatt
    26281 ttcggaatgt cgaacagcgc cagcaggttg tcatctttca gcaccttgtc ggttgcgtga
    26341 agatacatca gatgcagctc gttggtggct ttaattagct cctgctcggc actctcggta
    26401 atggtgtagt aatgatacgg atcctgattg atcacctgac cgttggcctg cacataggcg
    26461 ttttgtagcg gatctttttc atccagccat ttaccgtcaa actggccttt gttttccaga
    26521 cgcgctccac tgattttcag cagctcgcct gcaatttccg gctgcggtaa gctgtattca
    26581 gtatcttccg tctggatcat ccagcccaga atggtggtgt catcaaaagt gtctttaagg
    26641 gtatagcagc cgttttcgac caccatctcc agctcgcgcg tccactgttg cccttgcggc
    26701 aacggggaat gaatcacgtt ctgttccgca atacggactt tgttgccatg caattgggta
    26761 atgatggcga catggccagt gtctttaaat tcaccgcctt tatcccagat aagaagcgca
    26821 cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag gatgttgtca
    26881 ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc cacgtcagta
    26941 aagaccacac cgtaattcag aaagagaaaa cggcgagcaa attcaacgca ttgccacttg
    27001 tggcccatat attcgtcgtc gatatagcta cggaatacgg cgtcatcttc gtattcctgc
    27061 ggatcgagag aactgtaatc tgaagagtag attgctaccc cgcctggggc gtagcccaat
    27121 aatgtcccga acggggcatc ctggctggtc gttcctttgc tcatcacttt acctttaaca
    27181 atacaaccta agcagttggc gtaattgttg tgctctgatt acctgctcag cgacattaac
    27241 cggacagagg tcagactaat catacacctc atcgcagcgg ctggcgcaaa gggttaaaaa
    27301 attcacattc tgtacagcaa gtgacctgct acactgcttc aacactacca ctcagaaggc
    27361 aactcactat gacagacaat acttatcagc ccgcgaaagt ctggacgtgg gataaatccg
    27421 ctggcggcgc gttcgccaat atcaatcgcc cgttttctgg tccgacgcat gaaaaaacgc
    27481 tgcccgttgg caaacatcca ttgcaacttt attcgctggg aacgccgaac ggtcagaaag
    27541 taacgattat gcttgaggag ctactggcgc tgggcgttac tggtgcagag tacgacgcct
    27601 ggctgattcg tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa gtgaacccaa
    27661 actcgaagat cccggcgctg cgcgatcata cgcataatcc gccgatccgc gtgtttgaat
    27721 ctggttcgat cctgctttat ctggcggaga aatttggcta cttcctgccg caggatctgg
    27781 caaaacggac tgaaacgttg aactggctgt tctggttaca gggtgccgcg ccgttcctcg
    27841 gcggtggttt tggtcacttt taccattacg cgccggtgaa aattgagtac gccatcaacc
    27901 gctttaccat ggaagccaag cgcctgctcg acgtgctgga taagcaactg gcgcagcata
    27961 agtttgttgc gggcgatgag tacaccattg cggatatggc gatttggccg tggtttggca
    28021 acgtggtgct cggcggcgtg tatgatgccg cagagtttct ggatgcggga agttataaac
    28081 atgtgcaacg ctgggcgaaa gaagtgggcg aacgtccggc agtgaaacgt gggcgtattg
    28141 ttaaccgcac caacgggccg ctgaacgagc agttgcatga gcgccatgac gccagtgatt
    28201 ttgagacaaa tacagaagat aagcgtcagg ggtaagggtt ggtgttcgtc gcagcaagcc
    28261 atccaggccg gataaggcgt tcacgccgca tccggcaatc gtgcacaatg cctgatgcga
    28321 cgctgccgcg tcttatcagg cctacaaaag ctaatcatca cgcgctggta atgtcgtaat
    28381 ccatttggcg cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg
    28441 caaatccgac gtgtaccagc acccactggc ccagcagatc ggcagggtta ccttcgcaaa
    28501 tcagggcgat attcacgtcg cgcttgatac cacatacttc aacctgcgca agctggtgaa
    28561 tatcttcacc gacagccagc acctggcctg gaacgccaat acacattgtt aactccggtt
    28621 atttcgattg ccggacaaac acgaaaaaga acctttagcg ggtgttccct ctcaccctaa
    28681 ccctctcccc ggaggggcga ggggaccgtc cgtgcgcttt ttaggcgttg tcctcagtct
    28741 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
    28801 ccgtgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
    28861 cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
    28921 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
    28981 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg
    29041 ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgcccc tcctgcggcc
    29101 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttggtgactg gcaacgaaag
    29161 gatcattcgt gcgcagtcat ccgtcaggcg ttggccttct tcaatcgaca tgctgtgcga
    29221 aagcggcgac atcagcgaac aggagaggta ttgcgaaaca ccgtccagtt cgccaacggt
    29281 aaaggtcatg gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg
    29341 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
    29401 ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttgaaggatg
    29461 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
    29521 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga
    29581 tggcttccac gcctgattcg cgcagcgcag ccagaaccag ctcaagcgca ggttcaatca
    29641 ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag cgattccggg atcacgccga
    29701 ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat acgtcggcca
    29761 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
    29821 ggatcatcat cgttcccggc gtgttctttt tcgacacaat ggcatccgca ataatcagat
    29881 gatcgcgatt tgccatgtcg ctaagcagct ccattcccgc cgtgccgcca tcgaggatct
    29941 caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acgccgatgg
    30001 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
    30061 cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg
    30121 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcgg caaccggtgt
    30181 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
    30241 ccaggttgat ggaacaaccg cctggtagtt gctgatgata ccgtctttaa taaccatcca
    30301 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
    30361 cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
    30421 actgtattgg ttttgcagga tatcctgcaa ttcacagcag tgaacggtac gaccaataat
    30481 acggcccagc gtggagtgca gctgtgccac ttccagcgtg ttgccagtca gtttctgata
    30541 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
    30601 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gtgatttcac
    30661 ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca
    30721 cggcgcctgt ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
    30781 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
    30841 aatgtagccg cccgggaaca ggaagctgcc gtttttgctg tcggtcggga attccggcac
    30901 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcgat
    30961 aaccgcggta tcaaccttat aaacctgctc aacaaagtcg ctcagtttgt cgatgaaaga
    31021 cttgatgtac atcaggcgct caaggttcaa cacgcccaac ccgtcgaggt tgattgggtt
    31081 cgcgacacca ctcactgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
    31141 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag
    31201 gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag tagccattgg cgaaaatacc
    31261 caactgaccg ctggcaacca gatctttgat cttgttctga actttagtga actcttccgg
    31321 actgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc
    31381 ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat
    31441 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
    31501 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
    31561 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
    31621 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
    31681 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
    31741 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
    31801 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
    31861 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
    31921 gccaccaacg aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac
    31981 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag
    32041 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
    32101 gacagcttgt cgcgatagat aagctcgcca aagcgcagcc cgatgaaaat cgccagcaac
    32161 acactgatgg tgttggtcag cttgacgaac aggctctttt catccggacc gttgccacgc
    32221 agacccgcct gcaccagtga accttcaaag atgacaatcg agaagcccat gatgaacgcc
    32281 gtcagcagcg agaacagcgg caacatttca tagctctgcc agagcggatg caccttgtag
    32341 cccgccgaga tcatcagcga ccccattgaa gactggtgca tggtcggcag cagcgcaccg
    32401 agcgcgatga tgaagaacat caccttgttc agacgttgta gcgacacctt ccagcccaga
    32461 cgttcaaaca gtgccggagc aaactccaga gccatcacgc cgatgtagat ggtcatacag
    32521 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
    32581 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac
    32641 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
    32701 accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccaatcag caggtcaaac
    32761 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
    32821 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
    32881 ggtttactga tgattttgcc acccagcggt tgtggatcat gactcatgat cgtctcctcc
    32941 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
    33001 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
    33061 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
    33121 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
    33181 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
    33241 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac cgaaaatcac
    33301 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
    33361 acctttctgg ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
    33421 cttcggcacg ttgtacggac aggcgaccat gcagtaacgg caaccggtgc acacgtcttt
    33481 gtcgtaatgg acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga
    33541 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc
    33601 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
    33661 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggaa
    33721 gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta
    33781 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
    33841 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
    33901 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
    33961 accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa accaatagcg
    34021 cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc
    34081 tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc tttttcgtta
    34141 cagccatagc acgggtgacc aatcgccacc ggccacacac cgccaacatc gcagaattgc
    34201 agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag
    34261 ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
    34321 cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca
    34381 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc
    34441 gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac ttcttgcaag
    34501 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc ggagcaggaa
    34561 ccaatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc
    34621 tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccaccact
    34681 aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc gacctgatga
    34741 ccgaaggcgg cggaaagcac ttcgtgatat tccagagaga tagtctccag cacgaggttt
    34801 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
    34861 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg
    34921 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
    34981 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
    35041 gcgaggacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
    35101 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga
    35161 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
    35221 cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
    35281 aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg
    35341 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
    35401 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    35461 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacccc attcgttata
    35521 tatcgccata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca
    35581 tacttgttta agacgtaatc ccgtaagaga taaacctatc aggagagata tttatcgaac
    35641 catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    35701 tcattatgga atccgtgatt aacccccgga tagatataag cctcataaac cttattattg
    35761 tctttcaacg ccgcctcgta aggaggccag ccctcgttga ttcgggtgtc cagttccgcg
    35821 aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca
    35881 taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca
    35941 ccgccatagc aaaatccggt aatacccact ttacctgtcg cttgcggata gcgttgcata
    36001 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc
    36061 tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaatcc gtcaggtgcc
    36121 agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt
    36181 ccacgattct catgcaccac ccccacggct ggcgttttgc cgctcatttt tgcgggtttc
    36241 accagataac cccgtacctc gccgtgacca tttggcgaag gatacgtgat gtactcagca
    36301 acaatctccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa
    36361 tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
    36421 tcacgtttcg aaattttccc gtgaacgtaa tagtcgtagt aatccaacaa ctcttgtggg
    36481 aaatctttgg cggttaaacg cggcatcgtt gcattcctca actggtgttt ttttaagcaa
    36541 agcataagcg cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac
    36601 gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg
    36661 tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcagtaccgc tattgcggaa
    36721 aaagtggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta
    36781 acgcgctgga atcacagcgt gcaatcctgc gtaaagcgtt tgatttaggc attacgcact
    36841 ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac tttggtcgcc
    36901 tgctgcgcga ggattttgct gcttatcgcg atgaactgat tatctctacc aaggctggct
    36961 acgatatgtg gcccggcccg tacggctctg gcggttcacg taaatacctg ctcgccagcc
    37021 tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac tctcatcgcg
    37081 tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg
    37141 gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg
    37201 agttgctgca cgagtggaaa attccgcttt taattcatca accttcgtac aatttactga
    37261 accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgta
    37321 ttgcctttac tcctctggct cagggattgc tgaccggaaa atatctcaac ggcattccgc
    37381 aagattcacg gatgcatcgt gaagggaata aaattcgtgg tctgacgccg aaaatgctta
    37441 ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag cgtggacaat
    37501 caatggcgca aatggcgtta agctggttgc tgaaagatga tcgcgtgacg tcggtattga
    37561 ttggtgccag ccgcgcggag caactagagg agaacgtgca ggcgctgaat aatctgacat
    37621 ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggcgaa ctgaatctgt
    37681 ggcaggcgtc gtccgataaa tgacctgtta ataacgggcg cgcgaaccgc gcccgaatca
    37741 gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc agaaagcaca
    37801 aacgtcagat ggataatgac gtaccacatc agtttgttat ccgggacatt tttcgcatcc
    37861 ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt
    37921 ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt ctcggagata
    37981 tccagctgtg agacgaaatt ctcataaccg gaaaacatca ccatcaccag caaaccgcca
    38041 accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag
    38101 aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac
    38161 gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt
    38221 tcaaaaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa
    38281 ttgagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt cgcataattc
    38341 tgaggtttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag
    38401 gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aatgacccga ccacgcagta
    38461 ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg cgaagatgac
    38521 accgataccg gattgcggcg agaaaaccta tgttggtagc gctcgcctga aagatcgtaa
    38581 agcactggtg acagggggcg attccggaat aggtcgcgct gccgccatcg cttacgcgcg
    38641 agaaggggct gacgtggcga tcagttatct tcccgtggaa gaagaagacg ctcaggatgt
    38701 gaaaaagatc attgaagaat gcggacgcaa agccgttctg ctgccaggcg atttaagcga
    38761 tgagaaattc gcccgttcgc tggttcacga agcgcacaag gcgttaggcg ggctggatat
    38821 tatggcgctg gtcgccggga aacaggttgc cattccggat attgcagacc tcaccagcga
    38881 acagtttcaa aagacctttg ccattaacgt tttcgcgctg ttctggctaa cccaggaagc
    38941 gatccccctg ctaccgaaag gtgcaagtat catcaccact tcgtcaatcc aggcatacca
    39001 gccaagtccg catttactgg actatgcggc tacgaaggcg gcgattctga actacagccg
    39061 tggcctggca aaacaggtcg cggagaaagg tattcgggtg aatattgtcg cgccaggccc
    39121 tatctggacg gcactgcaaa tttccggcgg acaaacgcag gataagatcc cgcagtttgg
    39181 tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg tatatgttta
    39241 tctggcaagt caggagtcga gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga
    39301 gcatttaggt taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt gtgaattcac
    39361 tcttacctga tgtggcgaaa acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt
    39421 tgcaatagat tttgtaggcc tgataagcgt agcgcatcag gcattgagca cctaatgccg
    39481 gatgcgtcgt gaacgcctta tccggcctac aaacgcacga agattcaaca tgttgcaata
    39541 gattttgtag gcctgatgag cgtagcgtat caggcaattc tactttactt cgctttggcg
    39601 gtttcttcgc cgaccagacc tatcttcagg taacccgcct gatgcagcgt atccattacc
    39661 ttcatcaacg tctcgtaatc gacggttttg tccgctcgga agaagatggt ggtgtctttc
    39721 ttgccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac cggatcgtta
    39781 ccgataaaca tcgagttgtc tgccttcacc gacagataaa ccggtttttc cgggcgcggc
    39841 tgcggcgtgc tggtagaagc aggcaagttc accttcacat ctaccgtcgc taacggtgcc
    39901 gccaccataa agataatcag cagaaccaac atcacgtcga taaacggcgt cacgttgata
    39961 tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcattgccat cggacattat
    40021 cctgcgcgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc ttccagatcc
    40081 aggtcacggc tttgcagcaa caatacctgc gctgcaacat cacccagcat cgctttaaag
    40141 ccgccaatct ggcgtgcaaa tacgttatag ataacgaccg caggaatcgc tgccacgagg
    40201 ccgattgccg ttgctaacag agcttctgcg atacccggcg caacgactgc caggttagtg
    40261 gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt accaaacaga
    40321 ccaacgaacg gagaaatcgc gccgatggtt gccagatagc cgttaccgcg gcccatttga
    40381 cgacccactg cggcgacccg acgctccaga cggaagctgg tacgttcttt aataccttcg
    40441 ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag caaatgcagg
    40501 cttaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa ggaacgtgct
    40561 tcagccagca gttgctgctc gcgcttaagg cgacgcttct gattgaagaa ctctacgctc
    40621 ttactgaaga agattgccca ggtgactacg gaggccaaaa taagcccaat catcacgcac
    40681 ttaacgacaa tatcggcgtg ctgatacata ccccagacgg aaaggtccgt ctgcattaaa
    40741 ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgcgcata ataatatcaa
    40801 aacgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta cctttgcttt
    40861 cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga aaattttctg
    40921 ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag acatccagac
    40981 gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac tggtgaatgc
    41041 aggacgcagc aaaaaataca ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc
    41101 gctggtattt gagagtatgg aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga
    41161 gttgttctat ggacggcgcg gaacgttaac ccatttttcc ttacaacaag cgatgtgtga
    41221 actggaaggt ggcgcaggct gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc
    41281 gattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg cctatgaacc
    41341 gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat cgtggtttga
    41401 tccgctgatt ggtgccgata tcgttaagca tctgcagcca aacactaaaa tcgtgtttct
    41461 ggaatcgccg ggttccatca ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt
    41521 gcgcagtgtg gtgccggatg ccatcattat gatcgacaac acctgggcag ccggtgtgct
    41581 gtttaaggcg ctggattttg gcatcgatgt ttctattcag gccgccacca aatatctggt
    41641 tgggcattca gatgcgatga ttggcactgc cgtgtgcaat gcccgttgct gggagcagct
    41701 acgggaaaat gcctatctga tgggccagat ggtcgatgcc gataccgcct atataaccag
    41761 ccgtggcctg cgcacattag gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt
    41821 ggccgaatgg ctggcagaac atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg
    41881 cagtaaagga cacgaattct ggaaacgaga ctttacaggc agcagcgggc tattttcctt
    41941 tgtgcttaag aaaaaactca atgatgaaga gctggcgaac tatctggata acttcagttt
    42001 attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcaa atcaaccaga
    42061 acatatcgcc gccattcgcc cacaaggcga gatcgatttt agcgggacct tgattcgcct
    42121 gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg gttttgcacg
    42181 gattgtataa cattgccact tttggacaat tttgcagaca ttttattgtg aaaagtctta
    42241 aattgttgcg tccgggatca aggcgtcccg gacgattcag gagtacaata ggcagataaa
    42301 agcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc atcgctgcgc
    42361 tctggcaaca cgactttgcc gcgctggcgg atcctcatat tgttagcgtt gtttactttg
    42421 tcatgtttgc cacgctgttt ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg
    42481 acagcttgtt gatactggca ggcgcattga ttgcccaagg ggttatggat tttctgccta
    42541 cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat attcaggggc
    42601 gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct gctaaatatc
    42661 accagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg gctggacgtt
    42721 ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt ctgccaaacc
    42781 gccgcttcca gtttttcaac tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt
    42841 ttggctatgc cttaagtatg attccgttcg ttaaacgcca tgaagatcag gtaatgacgt
    42901 tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg
    42961 tgattaaaaa aaaatactgt aacgcctgac gattttcccc gttcccggct gctgtaccgg
    43021 gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc cggcgtcaca
    43081 ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc ga
//
LOCUS       DAJTYI010000018        41160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYI010000018 DAJTYI010000000
VERSION     DAJTYI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..103)
                     /locus_tag="OO564_000982"
     CDS             complement(<1..103)
                     /locus_tag="OO564_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652201.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIY"
     gene            complement(143..988)
                     /gene="glpF"
                     /locus_tag="OO564_000983"
                     /pseudo
     CDS             complement(143..988)
                     /gene="glpF"
                     /locus_tag="OO564_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1414..1659
                     /gene="zapB"
                     /locus_tag="OO564_000984"
     CDS             1414..1659
                     /gene="zapB"
                     /locus_tag="OO564_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6652202.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1744..2229)
                     /gene="rraA"
                     /locus_tag="OO564_000985"
     CDS             complement(1744..2229)
                     /gene="rraA"
                     /locus_tag="OO564_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6652203.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2322..3248)
                     /gene="menA"
                     /locus_tag="OO564_000986"
     CDS             complement(2322..3248)
                     /gene="menA"
                     /locus_tag="OO564_000986"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6652204.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3315..4646)
                     /gene="hslU"
                     /locus_tag="OO564_000987"
     CDS             complement(3315..4646)
                     /gene="hslU"
                     /locus_tag="OO564_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6652205.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4656..5186)
                     /gene="hslV"
                     /locus_tag="OO564_000988"
     CDS             complement(4656..5186)
                     /gene="hslV"
                     /locus_tag="OO564_000988"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6652206.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5279..6238)
                     /gene="ftsN"
                     /locus_tag="OO564_000989"
     CDS             complement(5279..6238)
                     /gene="ftsN"
                     /locus_tag="OO564_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6652207.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6330..7355)
                     /gene="cytR"
                     /locus_tag="OO564_000990"
     CDS             complement(6330..7355)
                     /gene="cytR"
                     /locus_tag="OO564_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6652208.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7511..9709)
                     /gene="priA"
                     /locus_tag="OO564_000991"
     CDS             complement(7511..9709)
                     /gene="priA"
                     /locus_tag="OO564_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6652209.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            9912..10124
                     /gene="rpmE"
                     /locus_tag="OO564_000992"
     CDS             9912..10124
                     /gene="rpmE"
                     /locus_tag="OO564_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6652210.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10185..10793)
                     /locus_tag="OO564_000993"
     CDS             complement(10185..10793)
                     /locus_tag="OO564_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6652211.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(10977..11294)
                     /gene="metJ"
                     /locus_tag="OO564_000994"
     CDS             complement(10977..11294)
                     /gene="metJ"
                     /locus_tag="OO564_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6652212.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11571..12731
                     /gene="metB"
                     /locus_tag="OO564_000995"
     CDS             11571..12731
                     /gene="metB"
                     /locus_tag="OO564_000995"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6652213.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12734..15166
                     /gene="metL"
                     /locus_tag="OO564_000996"
     CDS             12734..15166
                     /gene="metL"
                     /locus_tag="OO564_000996"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6652214.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15515..16405
                     /gene="metF"
                     /locus_tag="OO564_000997"
     CDS             15515..16405
                     /gene="metF"
                     /locus_tag="OO564_000997"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6652215.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16734..18914
                     /gene="katG"
                     /locus_tag="OO564_000998"
     CDS             16734..18914
                     /gene="katG"
                     /locus_tag="OO564_000998"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6652216.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19008..19913
                     /gene="yijE"
                     /locus_tag="OO564_000999"
     CDS             19008..19913
                     /gene="yijE"
                     /locus_tag="OO564_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6652217.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(19940..20557)
                     /locus_tag="OO564_001000"
     CDS             complement(19940..20557)
                     /locus_tag="OO564_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6652218.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20832..21924)
                     /gene="gldA"
                     /locus_tag="OO564_001001"
                     /pseudo
     CDS             complement(20832..21924)
                     /gene="gldA"
                     /locus_tag="OO564_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(21935..22597)
                     /gene="fsa"
                     /locus_tag="OO564_001002"
     CDS             complement(21935..22597)
                     /gene="fsa"
                     /locus_tag="OO564_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6652219.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22609..25110)
                     /gene="ptsP"
                     /locus_tag="OO564_001003"
     CDS             complement(22609..25110)
                     /gene="ptsP"
                     /locus_tag="OO564_001003"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6652220.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25419..26498
                     /locus_tag="OO564_001004"
     CDS             25419..26498
                     /locus_tag="OO564_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6652221.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26513..26833
                     /locus_tag="OO564_001005"
     CDS             26513..26833
                     /locus_tag="OO564_001005"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6652222.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            26884..29181
                     /locus_tag="OO564_001006"
     CDS             26884..29181
                     /locus_tag="OO564_001006"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6652223.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29147..30025
                     /locus_tag="OO564_001007"
     CDS             29147..30025
                     /locus_tag="OO564_001007"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6652224.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30027..30368
                     /locus_tag="OO564_001008"
     CDS             30027..30368
                     /locus_tag="OO564_001008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6652225.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30355..31206)
                     /locus_tag="OO564_001009"
     CDS             complement(30355..31206)
                     /locus_tag="OO564_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6652226.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31355..33088)
                     /locus_tag="OO564_001010"
     CDS             complement(31355..33088)
                     /locus_tag="OO564_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6652227.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33271..35922)
                     /gene="ppc"
                     /locus_tag="OO564_001011"
     CDS             complement(33271..35922)
                     /gene="ppc"
                     /locus_tag="OO564_001011"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6652228.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36274..37425)
                     /gene="argE"
                     /locus_tag="OO564_001012"
     CDS             complement(36274..37425)
                     /gene="argE"
                     /locus_tag="OO564_001012"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6652229.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37579..38583
                     /gene="argC"
                     /locus_tag="OO564_001013"
     CDS             37579..38583
                     /gene="argC"
                     /locus_tag="OO564_001013"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6652230.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38591..39367
                     /gene="argB"
                     /locus_tag="OO564_001014"
     CDS             38591..39367
                     /gene="argB"
                     /locus_tag="OO564_001014"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6652231.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39428..40801
                     /gene="argH"
                     /locus_tag="OO564_001015"
     CDS             39428..40801
                     /gene="argH"
                     /locus_tag="OO564_001015"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6652232.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            40985..>41160
                     /locus_tag="OO564_001016"
     CDS             40985..>41160
                     /locus_tag="OO564_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6652233.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLE"
BASE COUNT         9369 a        10570 c        11285 g         9936 t
ORIGIN      
        1 ggtagatttg ctcaaattcg cgctgcgaca cgctaatgat attggcatcg tgatccatta
       61 cgaccgcgcg ggagctggtg gtgccctggt cgagcgcaac gatatatttt tttcagtcat
      121 gtttaattgt cccgtagtca tattacagcg aagctttttg ttctgaagga gttgtggttt
      181 ccttttcttc cacaacacag atatcgcaag gcaaatggcg accaatcagt ttgcggtagg
      241 caaatgcacc tacaatcgcg ccaacgatag ggccgaaaag cggcaccagg aagtaaggaa
      301 tgtctctgcc gccggtaaag gcgacattgc cccagcccgc cagctaggca aagactttcg
      361 gaccgaagtc acgcgctggg ttcatggcaa aacctgtcaa tgggcccata gatgcgccaa
      421 tgaccgcaat cagtagacca atcagcaagg gagccaaagg gccgcgtggt acaccgttgc
      481 catcgtccgt taacgccagg ataagcccca tcagaatagc ggtaatcacc atctcaactg
      541 cgaaagcctg cacaaaattg atatgaggat tagggtaagt agagaaagtg ccagccagat
      601 caacactttc aacgctgccg cgaacaatgt gatgagtctg ctcgaagtcg aaaaataaat
      661 tgtagtaaag cccgtaaact aaagccgcag cgcagaaagc gccggcaact tgtgaaacga
      721 taaaaggaat aactttgcgc ttgtcgaaac aggcaaacag ccacaatgca atggtaacag
      781 cgggattaag atgcgcgccg gaaacccctg cggtcaggta gatggccatt gccaccccca
      841 gtccccaaat gacactgatt tcccactgac caaaagacgc accagcgact tttagtgctg
      901 caacgcaacc cacaccgaaa aaaatcaaca acccggtacc gaggaattca gcaatgcact
      961 ggcctttcaa ggttgatgtt tgactcataa tcggatcctg aagagttaat gtttgttgta
     1021 tgtgtgaaag tcacggacct ccacgatgct tgtaggcatg ctgttaactt atcgttaacg
     1081 agcaaaaacg agaaatatcg aacttaaaat gtgtgtgctt cgtcataaaa tgagcgttat
     1141 cgcgccattt attccatctg attgtacatg atgcatgttt gttagatcat tcgcgcaaca
     1201 aatttattaa cattttcgat tgatgtggat tatgtcacta ggagaaggat gaaaagtgtg
     1261 gcaaaccgta atctgcaagg cgtgccgctg gacagcgatg gcgcggcttc atacaatcgg
     1321 agctaactaa agtgcgctcg tatttattaa ggcgtcaccg gtaatcggga cgaggatttt
     1381 ttatccatca acgccttgca attcaggaga ggtatgacaa tgtcattaga agtgtttgag
     1441 aaactggaag caaaagtaca gcaggcgatt gataccatca ctctgttgca gatggaaatc
     1501 gaagagctga aagaaaaaaa caactcactg tcgcaggaag ttcaaaatgc ccagcatcag
     1561 cgcgaagagc tggagcgtga gaacaaccat ctgaaagaac agcagaacgg ctggcaggaa
     1621 cgtctgcagg ccctgctggg tcgcatggaa gaggtctgat tacgcttcat ctttcgcgta
     1681 atttctttac tgtgtaggcc aacaggtaac gcagaaaaaa ggcaccttgc ggtgcctttc
     1741 ttatcattca atatccagcg gatcttctga aagaataatc ccggtattgt cggcataaag
     1801 atggtcgccg gagaagaagg tgacaccgcc aaaattgacg cggacatcgc tttcgccaat
     1861 gccttcgcca gcggcaccaa ccggaattgc cgccatcgcc tggatgccga tatccaactc
     1921 ttccaggtca tctacctgac gcaccgcgcc gtaaatgacc agaccttccc attcattttg
     1981 tactgccaga cgcgccagtt cagcatcgac cagtgcgcga cgaacagaac caccgccatc
     2041 gacgacaaga acacgaccac ggccattctg ttcgagcaga tcgtacagca acccgttgtc
     2101 ctcgaaacat tttaccgtga ttatttgtcc gccaaacgac gcccgtccgc caaagttgga
     2161 gaacagcggt tccacgacgt taacatcttc ttgatagatg tcacaaagct cggaagtatc
     2221 gtatttcata ggcttaacat tcagttgctg cgagaatttt cagtatatcg cgctatgtgg
     2281 gctgttggca aagtcatcaa ttgttaattg atatttgtca gttatgctgc ccactggctt
     2341 aggaatatcc ctaaaacaaa cagcaggtta gtcagtaacg ctcccttgac agtacgttcc
     2401 agcattggtc gcatcgccac cgggtccatt tcccgcatca cataacgggc ttgcttcacc
     2461 agtaatggtg ccgccagcag gaacagccag ccccacaggc tatgcagcga aaagagatta
     2521 aacagcgcca gacacaccag cgagcccatc agcaggcagg catgataacg acgcgcgttc
     2581 acttcaccta agcgcaccac cagcgtgttt ttgccatttt cgcggtcgct attgatatca
     2641 cgcaggttat taatattcag tactgccgtt gccagcaggc cgcatgcggt cgccggaagg
     2701 atcagtgccg gaatcaatgt atgagcctgt aaataccagc tccccatgac actcaaccag
     2761 ccaaagaaaa ccagtacgga aatatcaccc agaccgatat aaccataagg acgattgccc
     2821 acggtgtagg tgatagcggc aatgatcgac aacccgccaa gaatcaggaa accgacaaaa
     2881 tcggccagcg tatggcatgc cactgcaacc agtgccagcc cggagagaca gatgagcacg
     2941 acggtaataa tgagcgcccg tttcatcttt tgctgggtaa tgaccccttt ttgcatgccg
     3001 cgtagcggcc caatgcggtc aggtttatcg ctgcctttta ccgcatcgcc gtaatcattg
     3061 gcgaggttag aaaggatctg taatagcccg gcggtaatta gtgccagcag ggcgaccagc
     3121 ggatcgaagt gaccttgcca ccatgccagc gccgtcccga cgataattgc agcaaaggcg
     3181 agggggaggg ttttaggtcg taaactttcc agccacgcct gagttcggct aatttgttgt
     3241 tcagtcataa tacgcgccaa taaaaatggg gcctttcagc cccatcaaac aatgatgaaa
     3301 atgattgaac gcgattatag gataaaacgg ctcagatctt catctgccac caacgcatcc
     3361 agatgtttgc tcacataatc tgcgtcaata gtgatatttt gaccgcttaa atcgctggcg
     3421 tcgtaggaaa tctcttccat taaacgctcc agaacagtgt gtaaacgacg agcaccgatg
     3481 ttttcggtag attcgttcac ctgccatgcc gcttccgcga tgcgtttaat accggagtcg
     3541 gtaaactcga tatttacgcc ttcagtcgcc atcagtgctt tgtactgcac ggtgatagag
     3601 gcattcggct cggtcagaat acgctcgaag tcgctggtgg tcagtgcctg cagttcaacg
     3661 cggattggca gccgaccttg cagttccggg atcaggtcag acggtttcgc aatctggaac
     3721 gcgccagaag cgataaacag aatgtggtca gttttgacca tcccgtgttt ggtggaaacg
     3781 gtgcaacctt ctaccagcgg cagcaggtca cgctgaacgc cttcacgaga aacatccgga
     3841 ccggaagact cgccgcgctt acagattttg tcgatttcgt cgataaacac gatcccgtgc
     3901 tgctcaacag cgtcgatagc gtcttgcttc agctcttccg ggttcaccag tttcgccgct
     3961 tcttcttcaa tcagcagctt catggcgtct ttgattttca gcttacgcgc tttttgcttc
     4021 tggccgccca ggttctggaa catggactgc agctggctgg tcatctcttc catgcccgga
     4081 ggagccataa tttcaacgcc catcggtgct gcggcaagat cgatctcgat ttctttgtca
     4141 tcaagctggc cttcacgcag ttttttgcgg aatgcctgac gagcagcgga aggttcctgc
     4201 tgctgttcag tctgtcccca gttgttttta gcaggtggga tcagcacgtc gagaatacgt
     4261 tcttctgcca gttcttcagc gcgataacgg tttttctcga tagcctggac gcgtaccatt
     4321 ttcacggcgg catcggtcag atcgcgaata atagaatcaa cttccttacc gacgtagccc
     4381 acttcggtaa atttggtcgc ttcaactttg atgaacggcg cattcgccag cttagccaga
     4441 cgacgggcga tttcagtttt accgacaccg gtcgggccga tcatcaggat atttttcggg
     4501 gtcacttcat ggcgcagctc ttcgttgagc tgcatgcgac gccagcggtt acgcagagca
     4561 atcgccacag aacgcttggc gttgtcctgg ccgatgatgt gcttatccag ttcgctgacg
     4621 atttcgcgtg gggtcatttc agacatggga gatccttacg ctttgtagct taattcttcg
     4681 atggtgtgga aatggttggt atagatgcaa atgtcgcctg caatatccaa cgccttttca
     4741 gcaatttcac gggcgctaag ttcagtgttt tctaacagcg cgcgcgccgc agcctgggcg
     4801 taagggccgc cggagccgat agcaataaga tcgttttctg gctgcaccac gtcaccgtta
     4861 ccggtgatga taagcgatgc agtttcatcc gcgactgcca gcagtgcttc aagtttgcgc
     4921 agcatgcgat cggtacgcca gtcttttgcc agctcaacgg cggctttaac cagatggccc
     4981 tgatgcattt ccagtttacg ttcaaacagt tcgaacagcg taaaagcatc cgcagtaccg
     5041 cccgcaaagc ccgcgatgac tttgtcgttg tacagacggc ggaccttttt cacgttgcct
     5101 ttcattacgg tattgcccaa cgtggcctga ccatcaccag cgatgaccac atggccgtta
     5161 cggcgtacgc ttactatagt tgtcacgagc tgaccccttg gttacgaata cagagtacaa
     5221 accccgtaca aaagtacggg gcataatgca attatagatg ggggggattt tgagggtttc
     5281 aacccccggc ggcgagccga atgcagtttg tatgacccgc catcttcaac cgattgaggg
     5341 tgctgtctgc gttctctttg cctttcaccg ggccaatgac cacacgattc cagccattgt
     5401 tggtggtgat tttcgagtca aatccttcga acgccagctg agcacgtacc gtctctgcct
     5461 gttccgcgcc tctgaacgaa ccgcactgca ccatccagcg gcgttcgtct tttttctccg
     5521 ccgtcggttt tggcgcgtca gcggcacgag caaccggcgc agcttgctgc ggcttcgact
     5581 gcgcggtcgt gtgcgcaggg gtttgcagca gatcctggta cggctgctga gtagaagctg
     5641 gtttagactg gcgcggttgc gcctgtaccg gcgcggcttg cgacgtacgc gtctgctgct
     5701 gccagctttg ttcagtcgta cgcgactgct gagccaggcg ttgctgctcc gctaattgct
     5761 gcgcctgacg ctggcgttgt agcgtttgct gacgctgttc cggcgtctgt tcattccacg
     5821 gaacctcaac cagctgcgtt ggctgctggc gcatatcagc ctgcatttgt tcaagaagct
     5881 gacgttgttc tggtgtcagt tgctccggcg ttttcacttc accaccggca gaaggttctg
     5941 tgggcgcacg cacacccggc tggcgacttt ccagctcttt aatgtagcgc cagcgttctt
     6001 ctggttttgg tggtagtccg tttccggtca ctttctggct ttgcagcgtc tcggactctt
     6061 ctttcttgtg atgcgtaatg aagtacagac caccgataaa ggtcacaaga acggcggcag
     6121 caatagcgac catagcggga gaaaccgcag gcagatttcg ttgctttttc cgtgaggtgc
     6181 tcttttttcg ccgcgaaggt gccggttggc tgcggcgtac ataatctcgt tgtgccacta
     6241 tcgtttcgct gtatttattc gttcgtcagc ccgccatgtt acttaagcgg cgggcctttg
     6301 accagtcacg gcagtcttaa aggtttactt taaggtaacg cgcgtgttga tccccggatg
     6361 ataagttcgc agtccattaa acgagagcca ctgccaacgt gttgcccctg catttgatca
     6421 agcaataaca gcatagcttc ccgaccgatt tcgtaacgcg gctgcgcgat ggttgtcagc
     6481 ggcggatcac aaaattgcgt caggtcgatg ttatcaaaac cgattatgga aaggtcttcc
     6541 gggactttca gcccctggcg ttttgcctga gaaagtgcgc cgagcgccat cacatcgcta
     6601 tggcagaaga ctgcagtagg cggttgtgga agatcaagca gctgttgcat tgctttgctt
     6661 ccggcttcga aggtgaagtc gccacgggcg atgtattgtg gatcaaccat aattccgcag
     6721 cgacgcagcg cctgaacata gccttgcagg cggtagtgac acagtggcat ctcttcggga
     6781 ccggctatac agccaatccg tttatgccct tgctcatata aataatttac tgcatcaaat
     6841 gcggcggtca gattgtcgat atgaacggta ggcagctcca gttccggtgc aaattcgttc
     6901 gccatcacca tcggcggcag attacgctgt tcctcaatgc tggcatcaaa cggcagcctt
     6961 gaacccagca gcaacatgcc atcaatttgc ttggtgatga tcaaatcgat aaaggttttt
     7021 tcctgctgat tttgatgcgc acagtcgcca atcagcacca gatatccgtg atttgccgcc
     7081 gtaacttcga taccgcgaat aatttcgcta aagaagggat cgcagatatc cgggacaatc
     7141 accagaatgg tgcgggattc attacgcttg acgttgcgcc ccataggctg cggtaaataa
     7201 cccacttccc gggccgcttt ttcaacccga ttacgggtgg cctgggagac tttatcggga
     7261 ttcattaatg ctcgggagac agtcgctgta gagacttttg ccttgagggc aacgtctttc
     7321 atggtcgcgg cagtttcctg cttcttcgct ttcacactca ctcctcgcct ggcacgtcag
     7381 gcgtactaca tccatgttta cttcacatcg gcaacatttt tagcagatag cgcgtgaaaa
     7441 cggttacaga attttcatga aaagtgtgat gaatattgaa tttttcgatc cgcctcgcat
     7501 cgtaagcggt ttaaccctca atcggatcaa catccagtac ccatttcacc ttgcgggaat
     7561 ccggtattgt attgatgagc gccagcgtac cactaatgat gtgttgcaag cgcacgcggg
     7621 aagggtgctg caacaatatc tgccagcgcc agcggccgcc acgtttaggt gccagtgccg
     7681 gaaccggacc gagaacccac agtttgtcgt ctgccagcgg actggagagg atcagattac
     7741 gcagttgttg caggaacagc ggcgcatgct gattgttatg atcttccgca cgcacaatca
     7801 catggctggt ccatggcggc agttgcatca tccgtcgctc agccagcgcc tgttcggcaa
     7861 aggcgtcgta gcctttatag agcaacgttt gcaacagagg atgttccgga tggtgcgttt
     7921 gcagcaccac ttcgccttgt ttacccgcac gcccggcacg accagcgacc tgggtgtaaa
     7981 gctgagcgaa acgctctgcc gagcgaaaat cggcagaaaa cagcgcgccg tccacatcca
     8041 gtaacgccac cagcgtcaca tccggaaagt ggtgaccttt cgccagcatt tgtgtgccga
     8101 tcaaaatcct cgcaccgccg cgatgtactt ccgccagttg ctgttccagc gcccctttgc
     8161 ggctggtggt atcgcggtcg atacgagaaa tgggcacatc agggaacagc ggcgcaagcg
     8221 tctgttcaag ctgttcggtg cccagcccca cgggtaccag gtgcgtggaa ccgcaggaag
     8281 ggcactggcg cggcaccgga cgctgactgt cacagtggtg acagcgcaaa tgctgttgcg
     8341 cctgatgcag cgtgtagtag tgatcgcaac gtgggcattc ggcaatccag ccgcagtcgt
     8401 ggcacagtag cgcaggcgca aagccacggc ggttaagaaa gaggataacc tgattgttgg
     8461 cctgtaaatg ctggcgcata cgggtgatta acgccggagc cagacctgcc tgcaccttct
     8521 gaccttttaa atccagcacg tgttgaattg ccggacgcgc attccccgcc cggcgcgtca
     8581 ggcgcagcag gcggtatttt ttctgctgga cgttgcacaa cgtttccagc gcgggcgttg
     8641 cggagccaag aataatcggg atttgctcgc tatgcgcacg atacaccgcc aggtcgcggg
     8701 catgatagcg ccagccttcc tgctgcttgt aggaactgtc gtgctcttcg tcaatgacaa
     8761 tcacgccgag atttttaaac ggcgtaaaca gcgcggagcg ggtgccgatc acaatcgccg
     8821 cctcgccatt tttcgctttc agccacgccg aaagacgctc gctgtcgttc agaccggaat
     8881 gcagaacttc cacgggcgca ttaaaccgtt cacgaaaacg ggcgatagtt tgcggtgtca
     8941 ggccgatttc cggcaccatc accagcgcct gtttgccctg agcgagcacg ttttccagta
     9001 cactgagata aacctccgtt ttaccggaac cggtaacgcc cgccagcagc caggcagaaa
     9061 aagtatctgc cgcgctatga attgcgccaa cggcggtggc ctgttcggta ttcaatcgca
     9121 accgctcacc agaaacggca tagttcgttc gccagtcgct aaactctggt gtttcacttg
     9181 ctaaatcaca cagacctttt ttgcgcagcg cctgcaacgc ggcatcatta aattcgagcg
     9241 tggcaacctg gtcgcgccag attttgcctt gccgtaacgc tgccagcgcc tgttgttgct
     9301 tgggtgagcg tttcaggctg ttcagatcca ccgcctgacc ttgttcagtg gcaaaccagt
     9361 accacatcgg cgcgttcgcc gcaggccgcc cctggcgtag taaaatcggc aaggcatgaa
     9421 acagcacatc gccaatcgga tgatgatagt aatctgccgc ccatagcagc aatcgccaga
     9481 cggagtgagt aaacaccggc tcaccatcca gcacttcgac taccgctttt agctcagtga
     9541 gcggcagttc gctggcatcg ctaactgata ccacaatccc gatgcgctcc tgctgtttgc
     9601 caaacggcac gcgcacgcga cacccagctt taaccgtcat gccttctggc agcagatagt
     9661 caaaggtacg aggaagcgga acgggcaagg caacgtgggc aacgggcata gcatcatcct
     9721 gacttgaaat tcggtgggtt agtatacaca ttgccgtaga agagtgcgga tcagtttgca
     9781 tacgctggtt aatttctgta tgatttcgcg ccttcgtacg aaatgatcgt attgaagcta
     9841 tacttttaac atcgcgtggt gtctggcgtt agggctggaa gagcgacgcg gccttaaacc
     9901 gaggttttcc catgaaaaaa gatattcacc cgaaatacga agaaattact gctagctgct
     9961 cttgcggtaa cgtaatgaaa atccgctcca ccgttggtca tgacctgaac ctcgacgtgt
    10021 gcagcaagtg ccacccgttc ttcactggca aacagcgtga tgttgctacc ggtggccgtg
    10081 ttgaccgctt caacaagcgt ttcaacatcc cgggcagcaa ataagaccgg atttccgaaa
    10141 aaagcgccgc acggcgcttt ttttgtgcct gaattttggt cgtatcacca cgaaaacagc
    10201 ggccactctt tggcaaccgt ggtgagttgt ggcgcgtcga aaacggcttg tggtgaaact
    10261 accgtttcct caagcggaat atttttaccg tagcgttctt tgagcttcgc ctggaccagc
    10321 gggttgctga gatcaaactc tttcaacttc tgctgttcgc ccacgcgcat tcccagcgcg
    10381 ttctggtaaa ccttccacac cacttccgaa cagtactggc gatcgtcgct ccatgagaag
    10441 ctgaaatcat acggtttacc gagataacgt tttgccgttt gcgtcagttt ttgctgttgt
    10501 tcaacactca gtccgccttc aacgcggcga acaacgtagt tgcccttttc accatgggca
    10561 atccactgct tgagcggggt gtatttcacc gggccgaccg cttcaaaaac gtagggcttt
    10621 ttgttacgca tcaccagcat accggtgtgg ctgtaatcgg aatgggttgc cagttggatc
    10681 gctttacttt gcgatgagcg agagatttga aagatgatgt cgccagtttg cggctgccag
    10741 gcaaaggcag gtacagaaac cagcaggctg aggatcagca gcctgttttt catagttaaa
    10801 cgtccatgta taaaaagcgg tgggtcgcag acaacgtgct cgttgtttat gccggatgcg
    10861 gcgtgaacgc cttatccggc ctacaagttc gtgcaaattc aataaattgc aatatgacgt
    10921 aggcctgata agcgtagtgc atcaggcgat tccactccgc gccgctcttt tccgctttag
    10981 tattcccacg tctccgggtt aatccccatc tcacgcatga tctcttttgc cgcttccggg
    11041 atttcgtcgc tgcgctcttt acgcagatcg gcatcatccg gcaaaggttg cccggtaaag
    11101 gcatgcagaa acgcttcgca cagcagctcg ctgttggtag cgtgacgcag gttgttcacc
    11161 tgacgacgcg tgcgttcatc ggtgaggatt tttaacacct taagaggaat ggaaaccgta
    11221 atctttttga cttgttcact cttcttgccg tgttcagcgt atgggctgat atattcgccg
    11281 ctccattcag ccatgagata cttaatcctc ttcgtcaata aattgagacc agaccccaca
    11341 atttatgtgg gtactgaccg taaacccgca tagtttaccg tacaggcgtt accgtgacat
    11401 cgtgtaatgc acctgtcggc gtgataatgc atataatttt aacggctatt tgggatttgc
    11461 tcaatctata cgcaaagaag tttagatgtc cagatgtatt gacgtccatt aacacaatgt
    11521 ttactctggt gcctgacatt tcaccgacaa agcccaggaa acttcatcac atgacgcgta
    11581 aacaggccac catcgcagtg cgtagcgggt taaatgacga cgaacagtat ggttgcgttg
    11641 tccctcccat ccatctttcc agcacctata actttaccgg atttaatgaa ccgcgcgcgc
    11701 atgattactc gcgtcgcggc aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac
    11761 tggaaggcgg tgctggcgca gtacttacta ataccggcat gtccgcgatt cacctggtaa
    11821 cgaccgtctt tttgaaacct ggcgatctgc tggttgcgcc gcacgactgc tacggcggta
    11881 gctatcgcct gttcgacagt ctggcgaaac gcggttgcta tcgcgtgttg tttgttgatc
    11941 aaggcgatga acaggcatta cgggcagcgc tggcagaaaa acccaaactg gtactggtag
    12001 aaagcccaag taatccattg ttacgcgtcg tggatattgc gaaaatctgc catctggcaa
    12061 gggaagtcgg ggcggtgagc gtggtggata acaccttctt aagcccggca ttacaaaatc
    12121 cgctggcatt gggtgccgat ctggtgttgc attcatgtac gaaatatctg aacggtcact
    12181 ctgacgtggt ggctggcgtg gtgattgcta aagacccgga cgttgtcact gaacttgcct
    12241 ggtgggcaaa caatattggc gtgacgggcg gcgcgtttga cagctatctg ctgctacgcg
    12301 ggttgcgaac gctggtgccg cgtatggagc tggcgcagcg caacgcgcag gcgattgtga
    12361 aatatctgca aacccagccg ttggtgaaaa aactgtatca cccgtcgctg ccggaaaatc
    12421 aggggcatga aattgccgcg cgtcagcaaa aaggctttgg cgcaatgttg agttttgaac
    12481 tggatggcga tgagcagacg ctgtgtcgtt tcctgggcgg gctgtcgttg tttacgctgg
    12541 cggaatcatt agggggagtg gaaagtttaa tctctcacgc cgcaaccatg acacatgcag
    12601 gcatggcacc agaagcgcgt gctgccgccg ggatctccga gacgctgctg cgtatctcca
    12661 ccggtattga agatggcgaa gatttaattg ccgacctgga aaatggcttc cgggctgcaa
    12721 acaaggggta aaaatgagtg tgattgcgca ggcaggggcg aaaggtcgtc agctgcataa
    12781 atttggtggc agtagtctgg ctgatgtgaa gtgttatttg cgtgtcgcgg gcattatggc
    12841 ggagttctct cagcctgacg atatgatggt ggtttccgcc gccggtagca ccactaacca
    12901 gttgattaac tggttgaaac taagccagac cgatcgtctc tctgcgcatc aggttcaaca
    12961 aacgctgcgt cgctatcagt gcgatctgat tagcggtctg ctacccgctg aagaagccga
    13021 tagcctcatt agcgcttttg tcagcgatct tgagcgcctg gcggcgttgc tcgacagtgg
    13081 tattaacgat gcggtgtatg cggaagtggt gggccacggt gaagtgtggt cggcgcgcct
    13141 gatgtctgcg gtacttaatc aacaagggct gccagcggcc tggcttgatg cccgtgagtt
    13201 tttacgcgct gaacgcgctg cgcaaccgca ggttgatgaa gggctttctt acccgttgct
    13261 gcaacagcta ctggtacaac atccgagcaa acgtctggtg gtgaccgggt ttatcagccg
    13321 taacaacgcc ggtgaaacgg tgttgctggg gcgtaacggt tccgactatt ccgcgacaca
    13381 aatcggtgcg ctggcgggtg tttctcgtgt aaccatctgg agcgacgtcg ccggggtata
    13441 cagtgccgac ccgcgtaaag tgaaagatgc ctgcctgctg ccgttgctgc gtctggatga
    13501 ggccagcgaa ctggcgcgtc tggcggctcc cgttcttcac gcccgtactt tacagccagt
    13561 ttctggcagc gaaatcgacc tgcagctgcg ctgtagctac acgccggatc aaggttccac
    13621 gcgcattgaa cgcgtgctgg cctccggtac tggtgcgcgt attgtcacca gccacgatga
    13681 tgtctgtttg attgagtttc aggtgcccgc cagtcaggat ttcaaactgg cgcataaaga
    13741 gatcgaccag atcctgaaac gtgcccaggt gcgtccactg gcggttggcg tgcataacga
    13801 tcgccagttg ctgcaatttt gctacacctc agaagtggcc gacagtgcgc tgaaaatcct
    13861 cgacgaagcg ggattacctg gagaactgcg cctgcgtcag gggctggcgc tggtggcgat
    13921 ggtcggggcg ggtgtcaccc gtaacccgct gcattgccac cgcttctggc agcaactgaa
    13981 aggccagccg gtcgagttta cgtggcagtc cgatgacggc atcagtctgg tggcagtact
    14041 gcgcaccggc ccgaccgaaa gcctgattca ggggctgcat cagtccgtct tccgcgcaga
    14101 aaagcgcatc ggcctggtat tgttcggcaa aggcaatatc ggctctcgct ggctggaact
    14161 gttcgcccgc gagcagagca cgctttcggc gcgtactggc tttgaatttg tgctggcagg
    14221 cgtggtggac agccgccgca gtctgttgag ttatgacggg ctggacgcca gccgcgcgtt
    14281 agccttcttc aacgatgaag cggtcgagca ggatgaagag tcgcttttcc tgtggatgcg
    14341 cgcccatccg tatgatgatt tagtggtgtt ggacgttacc gccagccagc agcttgctga
    14401 tcagtatctc gatttcgcca gccacggttt ccacgtcatc agtgccaaca aactggcggg
    14461 agcaagcgac agcaataaat atcgccagat ccacgacgcc ttcgaaaaaa ccgggcgtca
    14521 ctggctatac aacgccaccg ttggtgcggg cttgccgatc aaccataccg tgcgcgatct
    14581 gatcgacagc ggcgatacca ttttgtcgat cagtgggatc ttctccggca cgctctcctg
    14641 gctgttcctg caattcgacg gtagcgtgcc gtttaccgag ctggtggatc aggcgtggca
    14701 gcagggctta accgagcctg atccgcgtga cgacctctcc ggcaaagacg taatgcgcaa
    14761 gctggtgatt ctggcgcgtg aagcgggtta caacattgaa ccggaccagg tacgtgtgga
    14821 atcgctggtg cctgctcatt gcgaaggtgg cagcatcgac catttctttg aaaatggcga
    14881 tgaacttaac gagcagatgg tgcaacggct ggaagcggcc cgcgaaatgg ggctggtgct
    14941 gcgctacgtg gcgcgtttcg atgccaacgg caaagcgcgt gtgggcgtgg aggcggtgcg
    15001 tgaagaccat ccgctggcat cactgctgcc atgcgataac gtctttgcca tcgaaagccg
    15061 ctggtaccgc gataatccgc tggtgatccg tgggcctggc gctgggcgcg acgtcaccgc
    15121 cggggcgatt cagtcggata tcaaccggct ggcacagttg ttgtaatttc agaaatttaa
    15181 taatgcccgg tactcatgtt ttcgggtcta tggtttctaa tgaaatatat tgaattatca
    15241 tagaattagg ccggatgaag cgtttacgac gaatccggca agaagcaata agtacatggt
    15301 tagtttatat ttgcagtccg gtttgctttg catatcggat tttctttttc ttaccatcct
    15361 gaagtttttt catcttccct gatttttcct caccatcatt ggtcattttt cggttgacgc
    15421 ccttcggctt ttccttcatc tttacatctg gacgtctaaa tggatagatg ttcacaacac
    15481 aacatataac tacaagcgat tgatgaggta aggtatgagc ttttttcacg ccagccagcg
    15541 ggatgccctg aatcagagcc tggcagaagt ccaggggcag attaacgttt cgttcgagtt
    15601 tttcccgccg cgtaccagtg aaatggagca gaccctgtgg aactccatcg atcgccttag
    15661 cagcctgaaa ccgaagtttg tatcggtgac ctatggcgcg aactccggcg agcgcgaccg
    15721 tacgcacagc attattaaag gcattaaaga tcgcactggt ctggaagcgg caccgcatct
    15781 tacttgcatt gatgcgacgc ccgacgagct gcgcaccatt gcacgcgact actggaataa
    15841 cggtatttgt catatcgtgg cactgcgtgg cgatctgccg ccgggaagtg gtaagccaga
    15901 aatgtatgct tctgacctgg tgacgctgtt aaaagaagtg gcagatttcg atatatccgt
    15961 ggcggcgtat ccggaagttc acccggaagc aaaaagcgct caggcggatt tgcttaatct
    16021 gaaacgcaaa gtggatgccg gagccaaccg cgcgattact cagttcttct tcgatgtcga
    16081 aagctacctg cgttttcgtg accgctgtgt atcggcgggc attgatgtgg aaatcatccc
    16141 gggcattttg ccggtatcta actttaaaca ggcgaagaaa tttgccgaca tgaccaacgt
    16201 gcgtattccg gcgtggatgg cgcaaatgtt tgacgggctg gatgacgatg ccgaaacccg
    16261 caaactggtg ggcgcgaata ttgccatgga tatggtgaag attttaagcc gtgaaggagt
    16321 gaaagatttc cacttctata cgcttaaccg cgctgaaatg agttacgcga tttgccatac
    16381 gctgggtgtt cgacctggtt tataaatagt gtggcttttg tgaaaatcac acagtgatca
    16441 caaattttaa acagagcaca aaatgctgcc tcgaaatgag ggcgggaaaa taaggttgtc
    16501 agccttgttt tctccctcat tacttgaagg atatgaagct aaaacccttt tttataaagc
    16561 atttgtccga attcggacat aatcaaaaaa gcttaattac gatcaatttg atctacatct
    16621 ctttaaccaa caatatgtaa gatctcaact atcgcatccg tggattaatt caattataac
    16681 ttctctctaa cgctgtgtat cgtaacggta acactgtaga ggggagcaca ttgatgagca
    16741 cgtcagacga tatccataac accacagcca ctggcaaatg cccgttccat cagggcggtc
    16801 acgaccagag cgcgggggcg ggcacaacca ctcgcgactg gtggccaaat caactccgtg
    16861 ttgacctgtt aaaccaacat tctaatcgtt ctaacccgct gggtgaggac tttgactacc
    16921 gcaaagaatt cagcaaatta gattactacg gcctgaaaaa agatctgaaa gccctgctga
    16981 cagaatctca gccgtggtgg ccagccgact ggggcagcta cgccggtctg tttattcgta
    17041 tggcttggca cggcgcaggt acttaccgtt caatcgatgg acgcggcggc gcgggtcgtg
    17101 gtcagcaacg ttttgcaccg ctgaactcct ggccggataa cgtaagcctc gataaagcgc
    17161 gtcgcctgtt gtggccaatc aaacagaaat atggtcagaa aatctcctgg gccgacctgt
    17221 ttatcctcgc aggtaacgtg gcgctggaaa actccggctt ccgtaccttc ggttttggtg
    17281 ccggtcgtga agacgtctgg gaaccggatc tggatgttaa ctggggtgat gaaaaagcct
    17341 ggctgactca ccgtcatccg gaagcgctgg cgaaagcacc gctgggtgca accgagatgg
    17401 gcctgattta cgttaacccg gaaggcccgg atcacagcgg cgaaccgctt tctgcggcag
    17461 cagctatccg cgcgaccttc ggcaacatgg gcatgaacga cgaagaaacc gtggcgctga
    17521 ttgcgggtgg tcatacgctg ggtaaaaccc acggtgccgg tccgacatca aacgtaggtc
    17581 ctgatccaga agctgcaccg attgaagaac aaggtttagg ctgggcgagc acttacggca
    17641 gcggcgttgg cgcagatgcc attacctctg gtctggaagt agtctggacc cagacgccga
    17701 cccagtggag caactatttc ttcgagaatc tgttcaagta tgagtgggta cagacccgca
    17761 gtccggctgg agcaatccag ttcgaagcgg tagacgcacc ggaaattatc ccggatccgt
    17821 tcgatccgtc gaagaaacgt aaaccgacaa tgctggtgac cgacctgacg ctgcgttttg
    17881 atcctgagtt cgagaagatc tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag
    17941 cctttgcccg tgcctggttc aaactgacgc acagggatat ggggccgaaa tctcgctaca
    18001 tcgggccgga agtgccgaaa gaagatctga tctggcaaga tccgctgccg cagccgatct
    18061 acaacccgac cgagcaggac attatcgatc tgaaattcgc gattgcggat tctggtctgt
    18121 ctgttagtga gctggtatcg gtggcctggg catctgcttc taccttccgt ggtggcgaca
    18181 aacgcggtgg tgtcaacggt gcgcgtctgg cattaatgcc gcagcgcgac tgggatgtga
    18241 acgccgcagc cgttcgtgct ctgcttgttc tggagaaaat ccagaaagag tctggtaaag
    18301 cctcgctggc ggatatcatc gtgctggctg gtgtggttgg tgttgagaaa gccgcaagcg
    18361 ccgcaggttt gagcattcat gtaccgtttg cgccgggtcg cgttgatgcg cgtcaggatc
    18421 agactgacat tgagatgttt gagctgctgg agccaattgc tgacggtttc cgtaactatc
    18481 gcgctcgtct ggacgtttcc accaccgagt cactgctgat cgacaaagca cagcaactga
    18541 cgctgaccgc gccggaaatg actgcgctgg tgggcgggat gcgtgtactg ggtgccaact
    18601 tcgatggcag caaaaacggc gtcttcactg accgcgttgg cgtattgagc aatgacttct
    18661 tcgtgaactt gctggatatg cgttacgagt ggaaagcgac tgacgaatcg aaagagctgt
    18721 tcgaaggacg tgaccgcgaa accggcgaag tgaaatacac agccagccgt gcggatctgg
    18781 tatttggttc taactctgtc ctgcgtgcag tagcggaagt ttacgccagc agcgatgccc
    18841 acgagaagtt tgttaaagac ttcgtagcgg catgggtgaa agtgatgaac ctcgaccgtt
    18901 tcgacctgct gtaatctgac cttcttcagc gactgtcttt caggcagtcg ctgaagtttc
    18961 tttaccggcg tatagtgtcc acaggaaaac tacacactgg atctctcatg tctgccgcag
    19021 gaaagagcaa cccactggca atcagtggcc tggttgtgct cacacttatc tggagttata
    19081 gctggatttt catgaagcaa gtcaccagtt acatcggtgc cttcgacttt accgccttac
    19141 gctgcatttt cggcgctctc gttttattca tcgtcctttt attacgtggt cgcggaatgc
    19201 gcccgacgcc gtttaaatac accttagcca ttgccctgtt acaaacctgc gggatggttg
    19261 gtctggcgca gtgggcgttg gtcagcggag gtgcggggaa ggtggcgatc ctgagctata
    19321 ccatgccgtt ctgggtggtg attttcgccg cgttgtttct cggtgaacgc ctgcgacgtg
    19381 ggcaatattt cgcgattctg attgccgctt tcggcttatt tttggtgttg cagccgtggc
    19441 aactcgattt ctcttcgatg aaaagtgcca tgctggcaat cctctccggt gttagttggg
    19501 gggcgagcgc gattgttgct aaacgcctat acgcccgtca tccgcgcgtg gatttattat
    19561 cgttaacatc ctggcagatg ctgtatgcgg cgctggtgat gagtgtggtc gctttactgg
    19621 tgccgcaacg tgaaattgac tggcagccca ccgtgttctg ggcgctggcc tacagtgcga
    19681 ttctggcgac ggcgctagcg tggagcttat ggctgtttgt gctgaaaaac ttgcctgcca
    19741 gtattgccag cttaagcaca ctggccgttc ccgtttgtgg cgtactcttt tcctggtggc
    19801 tgctcggtga gaatccgggg gccgttgaag gtagcggtat tgtgctgatt gtgctggcac
    19861 tggcgctggt gagccgtaag aaaaaagaag ctgtcagtgt aaaaagcatc tgaatttttt
    19921 cttcatgtgg ggcgatctct tatttaacaa aataacgata atgccccacc atccgccagc
    19981 taaacagcac atcttcttcc tgcgcgcctg cgccaatgtt atgtatcacc agcggcgtac
    20041 cgtcgcgggc gaagccatct gacgccaccc caatatgcgc cagcccgtta tctaatcgcc
    20101 aggagacaat atcgcccgct tgatagtcac cggcattttt actgatgggg cgtgttttgt
    20161 tgtggcggct aaaccaggtt tccagattag gcacccgacg gtgatcgatg ttgctgtccg
    20221 ggcgctttaa cttccatttt tgcgggtact cagcaaaatt cttcgccata tcttcgtgaa
    20281 ccagtttctg caaatcgacc ttctggctgc gcaatgcgcg gatcaccaca tcggaacata
    20341 caccgcgttc ttgcggaaca tcaccgccag gataggtaag ctgcatatac gccggatcgt
    20401 aaaatagcgt gctgccaatt tgctgtctgg caccgtctgc gatggcaagg ttggtattgg
    20461 cctggatttg taccacggtt ggtggaacgg cgggagattt taaggagtgg ctggtaaatg
    20521 ccgttagcag gctgagcagc gccagtgaag ctttcatctg gttcgtccgt gaaaataaga
    20581 gtcacagatt cgaccttccg ggctaacgat tgttctgtgg agaaaatgtc cagaagcgga
    20641 taaaaaactc ctgaacaacg tgggagtttc agattactgg cacagagtgc cggatgcgga
    20701 gtgaacgcct tatccggcct acaaaagcac gcaaattcaa tatattgcag agattgcgta
    20761 ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag
    20821 tttggagtag gttattccca ctcttgcagg aaacgctgac cgtactggtc agctaccagc
    20881 agtgcggcgt aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacct
    20941 tctgcacaag atgcttttgc aacaatgcgc attttcgccg ggacatcttc tttaatatcc
    21001 agttgagcga gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt
    21061 tcctccaccg gcgcgttttc cagtaccagc tgcgtcagcg taccaaatgc cactttttca
    21121 ccgtgataat agtgatgcgc gtccgggata gcggtcaggc cgttatgcac cgcgtgagcc
    21181 gccgctttca aaaccaacgc cgctcaaata ggtgttcgct tcaatcacgc gctccagcgc
    21241 cggggttact acatgctgtt cggcggcaag catcgctttt tcgccttctt ccagcagggt
    21301 gttgtagcac agttcagcca gtgccagcgc cgcctgggtg cacttgccgc ccgccatggt
    21361 ggtcgcgccg ctacgagagc aagcacgcgc ttcaaaccag gttgccagcg catcgccgat
    21421 acccgctgct aacagacgtg caggtgcgcc cgcgacgatt ttggtgtcga caatgaccat
    21481 attcgggtta tttggcaaca gcagatagcg gtcaaactca ccctcatcgg tgtagataac
    21541 agacaatgcg ctgcacggtg catcggtaga ggcgatagtc ggtgcgatcg ctaccggaac
    21601 acccatgaaa tgtgccagtg ctttggcagt atcgagggtt tttccgccac cgataccgag
    21661 aattgcgcca cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga
    21721 acattcaccg ccaaacggcg taatttctac taccagtcca gcatctttaa agcttttctc
    21781 gacagtggat tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttctgc
    21841 cagcggcttc aggtattcgc ccagacgatt aatcacatca gcgccctgga tgtatttacc
    21901 cggtgattga ataatgcggt ccataattgc tcctttagag atgagtagtg ccaaatgcgg
    21961 cattccagtc gtgttcgaac ttctctatag ctgactctac tgcaggggtg ttgagcattt
    22021 gttgcgctac atctaagggc agggtgatgg attcacatcc tgccagtaaa cagtccagcg
    22081 cctgacacgg cgttttaaag ctggctgcca gcaccatgct ttctggcgcg tgcatttcta
    22141 acagcgtttg cagctcctga accgtacgaa tgccgtcccc gccctgggca tctacgcggt
    22201 taacatacgg agcaacgtat tttgcccctg ccagtgcggc taataaccct tctgcggcgc
    22261 tatatacagc ggtgccaagt gtagtaatac cctctttttt cagtatttta attgctgcca
    22321 gaccttcgga agtcaccggg attttcacca caatacccgg aatagcgtcg cgcaggcgct
    22381 tcgcttcttc caccatcccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct
    22441 catcaccaat cgctttttgc agacgcggca gcacttccca gatggactcc ttgctggcag
    22501 cgataatgct cgggttagtt gtcaccccgg caatcgggaa tatgcgtgcc agacgttcga
    22561 cttctgcgac gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag
    22621 ttcatgttgc agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcgttacg
    22681 gaattcttcg tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatgt gctgatcgcc
    22741 cgcagcgtgt ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg
    22801 caccggcgct tgcagacgcg ccacgctgat ggtggattgc tcaatgtgtt ctgatttgct
    22861 gtgtggaatg gcaaaactaa agcccagacc ggtagagaaa acggcctcgc gcgcccataa
    22921 gtcggcttcc agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat
    22981 gcctttgagc acttcttctt tgctgcgcca gtcactttcc agcgtgatgc actcggcggt
    23041 gaccagcggt gcgtcctgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag
    23101 cgaagtacgg caggccattg cctggttgag caacttgcgg cactcacggc tatcaagttg
    23161 cgccatccgc gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc
    23221 taagccgacc agcaacggca gcacggaacc tttcgctccc agctcaccgc acagaccaat
    23281 ccatttaccc tggcgatgca ccgcctgcac ggcgtaatcg agcgcccgca agaatgccgg
    23341 attcaggctg ttgtagtgac gagtaacctt agcgttatcg cgatccactg ccagcagata
    23401 ttgcgtcagg tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat
    23461 gatgaacatc accgacggca cttccagcat aatgccgagc tggatcttct catcaaacgg
    23521 aatgtgttcg ttacgtagtt gctgtttggc ttctgccagt ttttctttca cccataagat
    23581 ctcttccatt gaggagatca tcgggatcat gattttcagg ctgccgtgag cggaggcgcg
    23641 caggatcgag cgtagttgtg tggtaaataa cgacgcgtat tcttcataaa tacgcacggc
    23701 gcgataaccg aggaacgggt ttgcctctgc gggaatgttt agataatcaa cgggtttgtc
    23761 gccgccaatg tccatagtgc gcacaataat gctgcgtccg ttggcagact ccagcgcctg
    23821 acaaaaaatg ttgtacagct cgctttcgcc cggtgcactg gtgcgatcca tatagagcat
    23881 ttcagtgcgg aacaaaccaa cgccttccgc accattgccg aatgcggcct gcgcttccac
    23941 ggagtgagcg atgttagcgg caatttcaat gcggataccg tcagcggtac gggcttgttg
    24001 ggtcagccag acacgctgtt gctcacgcag ggcgtcctgt acgcgggctt cttgctgata
    24061 ataacgagct acggcttctc ctggctcaac cacaatcgcc ccggcgttgc cgtcgatata
    24121 aatcgtttga tgctgccacg gagtgagggc atcaatatcc acaccaacca gcgtcggaat
    24181 gttgaacgaa cgggcaagga tcaccgtatg tgaggtggtg ccgccgcttt tcagtaacaa
    24241 tcctttgagg tgatttttat ccagttcgag gaactggctg ggggtcagtt catcagccat
    24301 acaaatggcg gactgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg
    24361 ctggagtaac tggaagcaga cgtcgcgtac gtccagggca cgttcttgca ggtatctgct
    24421 gctggaacgg gcgaactctt cgcaaaagtg attcgcactg gcgacaatcg cttcggcgca
    24481 gcttaatccg gcgctgactc ctgccagtaa atgttcgtgc agggaagtat cgccagccag
    24541 ggatcggtgc gcttccagaa tcgcgctggt agcaccgtcg ctatccagca gacgaaactc
    24601 aatgtttttc agtaccagcg tcaggccgtt ttccagtgcg gattgctcgg cgtcaacgtc
    24661 tttggctgcg ggaagattac tcagcgcatt gagatctaaa gaagagatcg gcgtcagaat
    24721 gccgcctgca ctaccgctgc acacggtgcg gacgcggata atttgcggat tcagattggt
    24781 cagtgaaacc ggcagtggtt ccagttcgtc agatttaact tccgccagcg gcgcatcgca
    24841 gtgggggaat tcatcgcgca gccattggct taaacgctgg tgcccttcct gttcgtcggc
    24901 cccggaaatc agtagctggc agttatcacc caccagcgta tcggtgccaa tcagcgccag
    24961 agcgcttttg gcgttgcctt tacggtcagt acgcaggtta tgccactcaa tttgagatga
    25021 aaaagtatta cacagcgttt caacgtggct tgccggacgc gcatgtacgc cgttaggtag
    25081 ctcacaaata aattccacaa tcagggccat tgcctttctc ccggtacggt tttctgttta
    25141 caggataaga gcgcacggta acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa
    25201 tgtaatgtaa ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcatgcttcc
    25261 ggattatcgc aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta
    25321 aatggccttt ttatccactg tttgctccgc ctgcgattgc ctattgtttc ctccatcaaa
    25381 aatatggaca tgggcttttg caagcccggg agcagcttat gaatgagttg gtgcagatcc
    25441 tgaaaaatac ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat
    25501 cgggcggtat tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg
    25561 ccgtagccga tccgaatttg aaaaaactgt ttgatattgg cgttgcaggt ttgacgctga
    25621 tggtgccttt cctcgccgct tacattggtt actccattgc agagcgttct gcgctggctc
    25681 cgtgcgctat cggtgcctgg gttggtaaca gctttggtgc gggcttcttt ggtgcgctga
    25741 tcgccgggat tatcggcggc atcgtggtgc attacctgaa gaaaattccg gtgcataaag
    25801 ttctgcgctc ggtgatgccc atcttcatta ttcctatcat cggcacactg attaccgcag
    25861 gcatcatgat gtggggcttg ggcgagcctg taggggcgtt gaccaacagc ctgactcagt
    25921 ggcttcaggg gatgcagcag ggcagcattg ttatgctggc ggtgatcatg gggctgatgc
    25981 tggcgttcga tatgggcggt ccggttaaca aagtggccta tgccttcatg ctgatttgcg
    26041 ttgctcaggg tgtttatacc gtggtggcca ttgctgccgt gggtatttgt atcccgccgc
    26101 tggggatggg gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa
    26161 ccggcaaagc ggcactggtg atgggctgtg ttggcgttac tgaaggggcg attcctttcg
    26221 ccgctgccga tccgctgcgt gtcattcctt ccattatggt cggttcagtt tgtggtgcag
    26281 taactgcggc gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc
    26341 cggtggttga aggtaagttg ggttatatcg cagcagtggc tgtcggagca gtggtgacgg
    26401 ctgtttgtgt gaacgtgctg aaaagtctgg cgcgtaaaaa tgggtcttcg actgatgaaa
    26461 aagaagacga cctggatttg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa
    26521 aattattgca gtaaccgcat gtccttcagg tgttgcccat acctatatgg tagcagaggc
    26581 gctggaaagt gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc aggggtcaat
    26641 tggtctggaa aatgaactga ctgcggaaga cgtggcgagc gctgatatgg tgattctgac
    26701 caaagatatc ggcatcaagt ttgaagagcg ttttgctggc aagaccatcg tgcgcgtcaa
    26761 catcagcgat gcagtaaaac gtgctgacgc cattatgagc aaaattgaag ctcacctggc
    26821 gcaaaccgct taagttcttc tcccgctcgc aagggcgggt tcgctttccc acaggagttc
    26881 ctcatgacga atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc
    26941 tcgctggagc gggcgctgct ttataccgcc agccatcggc aaaccgaagg cgaaccggtg
    27001 atattgcgcc gggcgaaagc aacagcgtat atccttgaac atgttgaaat ttcgattcgt
    27061 gatgaagaac tgattgccgg taaccgcacc gtaaaaccgc gcgccgggat tatgtcgccg
    27121 gaaatggacc cttactggct gctgaaagag ctggatcaat tcccgacgcg tccgcaggac
    27181 cgctttgcta tcagcgaaga agataaacgt atctaccgtg aagagttgtt cccgtactgg
    27241 gaaaaacgtt cgatgaaaga tttcatcaac gggcagatga cagatgaagt aaaagccgcg
    27301 accaacacgc agattttcag catcaaccag acggataaag gccaggggca cattattatt
    27361 gattacccac gcctgttgaa tcacggtctg ggtgagctgg tggcacagat gcagcaacat
    27421 tgtcagcaac agccggagaa tcacttttat caggccgcgt tgttactgct ggaagcctcg
    27481 cagaaacaca ttttgcgtta cgccgaactg gcggaaacga tggcggcaaa ctgcacagat
    27541 gcccagcgtc gcgaagagct gctgactatt gcggagatct cccgccataa cgcgcaacat
    27601 aagccgcaga cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa
    27661 tacgaatcca acgccagttc gctgtcttta gggcgcttcg accagtatat gttgccgttc
    27721 tatcaggcat ctttaaccca gggcgaagat ccggcgttcc tgaaagaact gctcgaatct
    27781 ttatgggtga aatgcaacga catcgtgctg ttgcgctcca ccagtagcgc gcgttatttc
    27841 gccggtttcc cgaccggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc
    27901 gcggtgaacg tgctttcgtt cctttgcctt gacgcctatc aaagcgtgca attaccgcaa
    27961 ccgaacctcg gcgtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgcc
    28021 gaaaccattc gcctcggcac cggtattccg caaatcttta acgatgaagt ggtggtgcca
    28081 gcgttcctca accgtggcgt ttcgctggaa gatgcgcgcg actattccgt agtgggctgt
    28141 gtggaattat ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaatctg
    28201 ctgaaagtga tggaaatctg cctgcatgaa aatgaaggca atgctgcgct gacttatgaa
    28261 ggtttactgg agcagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc
    28321 agcaatattt gtgatatcgg ccatcgcgac tgggcacctg taccgctgct ctcgtctttt
    28381 atcagcgatt gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc
    28441 tccggcgtac agggaattgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg
    28501 atggtttttg agcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac
    28561 tttgcaacgc cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt tgagaaatac
    28621 ggtaacgata tcgacgaggt ggataacatt agcgccgaac tgttgcgcca ctactgcaaa
    28681 gaagtggaaa aataccagaa cccgcgcggc ggttacttca cgccgggatc gtataccgtt
    28741 tctgctcacg ttccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccggg
    28801 gaacaactgg cagacggcgg cttgtcacct atgctgggtc aggacgcaca agggccaacg
    28861 gcggtactga agtcagtcag taagctcgat aatacgctgc tgtctaacgg tacgttgctg
    28921 aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac
    28981 ttcttacggg tgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc
    29041 gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt
    29101 gctggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc
    29161 cggacagcgc atcagctgta acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt
    29221 caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa
    29281 aggctgtccg catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca
    29341 gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga
    29401 atgcccctcc ggggcgtttg aacggattgg tcgcgatatc agccttgacg ctctggaacg
    29461 ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg
    29521 cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt ttacagcgac tgcggctgtg
    29581 gggtgtctca tgcgccattg aaactgccgg agacgcgcca gcaagcaagc tgttaccgct
    29641 ggcgaaattg tgcgatgaag tgctgttcga tttaaaaatt atggacgcga ctcaggcgcg
    29701 ggatgtggtg aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga
    29761 gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt ttcacgctca gccgggagaa
    29821 tatgcagcag gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc
    29881 gtttcatcag tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga
    29941 ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg
    30001 atttcaggtt accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa
    30061 gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc cagttagaga
    30121 agtggggcgt cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg
    30181 aggatatccg tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg
    30241 agcgatttga acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc
    30301 agcgggtaat gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc
    30361 tggagtagtc ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga
    30421 gtgttcggtg tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc
    30481 gtgcgccact tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag
    30541 tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga
    30601 gagatagttt ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc
    30661 atagcgttcg tcgatataat cgcgaatagc ttcaaatagt gcctggctgc gtgaggcggt
    30721 ctggatttgg ctgccgcgca aatcgcggca gtggctaagc aggctggcta cgataagcct
    30781 tgccgtttgc tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga
    30841 acctatgcgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc
    30901 atcccattgc acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtggt
    30961 agcgggtgtt ttccattgtg ggaaattcca gccaccagcg gcaacgtaca gcacatcgcc
    31021 gggaactaac gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag
    31081 ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta
    31141 aatctggcgc agggacagcg ggccgttgat caggcgggag agcaaatagc tgacgtcgtg
    31201 atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt attaaaaaag
    31261 gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gtatcgcatc
    31321 cggcaaatag cgccagacgc tatacaaagg tcatctactg attacccacc tgatcgccat
    31381 acggcagagt gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca atccagcggg
    31441 tagtttcttt gaactgcgga ttcaccactg aacgggttgg atcgtaaccg tcgtaagata
    31501 agcccgccaa atctgaccag gtgtggatca gttccgccag gctgtattta cgatcgacat
    31561 cctgtgagaa atcacgggga tgagtcgctt gccatttttc cgacgtccac agcaggaacg
    31621 gaatggtgta catgtggcgc gtcgggttgt cttcgttacg cccctgagtt ttgtgcggcg
    31681 gcgtgtcgta aacctcttca ccgtggtcag agaagtaaac caggaatccg ttcgggtctg
    31741 ccgctttaaa gtctttaatc aggctggcaa ccacatgatc gttatacagg ttagcgttgt
    31801 cataatcgtt atatgattcc agctcttctg cgcttaatcc tggaggaaca tgatcggtat
    31861 tgccatcaaa cttgccctga ttttccgggt agcggtattt gtatttgata tgcgtaccca
    31921 gcagatgaac gatgatcagt ttcttcggcg cagggtcatt cagcacatcc tggaacggct
    31981 tcagcacgtt ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc atgtagtact
    32041 gcttgtcggt ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc atcgtctgct
    32101 ggttggtgat ccagaaggtt ttataacccg cctgtttcat catgttcatc agcgacggct
    32161 gcgtcagata cagatccggg ttcttttcat tggcaaaggt cagcgcctgt tgcaggattt
    32221 caatggtgta cggacgagac gtaactacgt tattaaacac ggtcagattc ggatcggttt
    32281 tatgcagcgc atccagctcc ggcgtggttt cacgcggata accgtacaga ctcatgcgtc
    32341 cgcgctgggt cgactcgcca atcaccagca ctaaagtgcg cggttcgtta cccgattcat
    32401 ctttgaaatt agccagtggc ggcaaggcat tattttcatt cagtaacttt gttagcgagt
    32461 ttagttgctg acgatactga taatagccgg tcaggaattg ccacggtgcg gcaggctcca
    32521 tacgcgaggc taggttatcc aacgttttct caaacggctt gtttttgata aacgtattca
    32581 tggcgatcgg atgcagaatc aagccataaa gcagggcaaa agagacaaca taacgccacg
    32641 gctttggaat atagaccggg cgcaggcgtg tccacagcag aactgccacc gccgtatagg
    32701 ccagcgcgat aagcacaatt ttcaggctga aatactggct taaatactcg ctggcttcgt
    32761 tggtgttggt ttcgaacatc acaaacagaa cgctctgcga gaactcctgc ccgtagatga
    32821 cgtagtagca cagcgccgcc agagaggccg tccatagcac cacgccgatc actgcggcaa
    32881 taattttaat ccgcttcgga aagaggaata ccgggatcaa ccacagcgag ctgaataaca
    32941 gcgagtcgcg aatgccgttg gtgccactat aaccactgat gtaaataatg gcctgtagca
    33001 gagtagagaa aaaccaaaag tagagcagtg cccaacccag ggctttccag ctagaaaggg
    33061 gtttagcctg gacttctgtg gaatgcatag taagaacctg tcttgaaaaa aatatcgccg
    33121 aatgtaacga caattcctta aggatatctg aaggtatatt cagaatttga ataaaatgca
    33181 gacagaaata tattgaaaac gagggtgtta gaacagaagt atttcagaaa accctcgcgc
    33241 aaaagcacga gggtttgcag aagaggaaga ttagccggta ttacgcatac ctgccgcaat
    33301 cccggcaata gtgaccatta acgcttgttc gacgcgagga tccggttcct ggccttcttt
    33361 ttctgcctgg cgggagcggt gcagcaactc ggcctgcaat acgttcagcg ggtcggtgta
    33421 aatattccgt agctgaatag actctgcaat ccacggcaga tcggccatca gatgggaatc
    33481 gttggcaatc gccagcacca ctttgatgtc ttcttcttgc aggttgcgta actctttacc
    33541 taacggccac agtgctttgt ctaccagacg ttgatcatag tattccgcca accacaggtc
    33601 tgctttggcg aagaccatct ccagcatgcc gagacgcgtc gagaagaatg gccaatcgcg
    33661 gcacatggct tccagctcgc tctgtttgcc atcttcgacc actttttgca gcgccgtacc
    33721 tgcacccagc caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa tccacggtat
    33781 ggcgcgcagc gactcgacgc cgccggttgg gcgacgtttc gccggacgtg aacccaacgg
    33841 cagtttgccc aattcttgtt ccggcgtagc ggagcggaag taaggcacaa aatctttgtt
    33901 ttcacgtacg tagccacggt agagatcgca ggagatgact gacagttcat ccataatgcg
    33961 acgccagctc tctttcggct caggtggtgg cagcaggttg gcttccagaa tcgccccggt
    34021 gtaaagcgac aggctgctga cggtgatttc tggcagacca tatttaaagc ggatcatctc
    34081 gccctgttca gtcacgcgca ggccaccttt caggcttcct ggcggttgtg acagcagcgc
    34141 cgcatgggca ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga acaacgtcag
    34201 ctcaataccc gctttttcgc aggttttgat taatgcatcc tgtgcctgat attgcgccca
    34261 ggaagctgcc atcacgcccg catcttttgc tgagtcggaa tagccaatca tcaccatctg
    34321 tttgccctga atcaggccgc gataccagtc gatattgagc agctgggtca tgacatcgtt
    34381 ggcgttgttc aggtcatcga gggtttcaaa cagcggagca accggcatcg caaacccgat
    34441 acccgcttct ttcagcagca ggtggacagc cagtacgtcg gacggcgttt tcgccatcga
    34501 gatcacgtag gcggcaatgg agccttgcgg cgcttcggca atcacctggc aggtatcgag
    34561 cacttcgcgc gtttcggcgc ttggttgcca gttgcgcggc agaagcggac gtttggagtt
    34621 cagttcgcgg atcaggaacg cctgtttgtc ggcctctgac cagctttcgt agtcgccgat
    34681 accgaggtag cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct cctgacggat
    34741 atcaatacgg accagcggta cgccgaaaca tttcacgcgg cgcagggtgt cgagcagatc
    34801 gccgttggcg ataataccca tgccacacgc ctgaagtgac tggtagcaag cgtagagcgg
    34861 ttcccacagt tcttcgtttt gtgtcagcag cccttctggt tttggcagtt cttcgccttt
    34921 caggcgcgct tccagccatg cctgtgtcgc catcaggcga gaacgcaggt ttttcatcag
    34981 atagcgatac ggttctgcgg caccttcttc gccaaccagc gccagcagtt caggggtcgc
    35041 ttcaaccatc gacagttcag aaaccagaac ctgaatatct ttcaggaaca gatcggtggc
    35101 tttccagcgg ctgagcagca ggacgtggcg ggtgatatcg gcagtgacgt tcgggttgcc
    35161 gtcgcggtca ccgcccatcc acgaggtaaa acgtaccgga acaaattcga cgggcagttt
    35221 gtagtcgaga ttctcttcca gttgttcgtt cagttcgcgc aggtaattcg gtacgccttg
    35281 ccacaggctg ttttctacta cggcaaagcc ccatttggct tcatctaccg ggcttggacg
    35341 cagcttacgg atttcatcgg tatgccatga ctgggcgatc aactggtgca ggcgacgcat
    35401 cagctggttg tgttcgtagt cagcgatatc tttgttatcg agctgtttta aacaggcgtt
    35461 cacttccacc attttgtgga tcagtgtacg acgggtaatt tcggttgggt gagccgtgag
    35521 gaccagttcc agcgacagcg attccactgc ttttttgatg gtgtcttcgc tcagttccgg
    35581 ctggtttttc agtttacgca gggtgcgggc gatcacttcc gggttgctgg cagcttcgcc
    35641 tttcggcgaa atgctgtggt attgctcggc ggtgttggcc aggttcagga actgactaaa
    35701 cgcacgcgca acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg tgagcaactc
    35761 ctggcggtta gcatcattgc cagcgcgtga agatttcgac aacttacgga tagtttctac
    35821 gcgttcaaga atgtgttctc ccaacgcatc cttgatggtt tctcccagca ctttgccgag
    35881 catactgaca ttactacgca atgcggaata ttgttcgttc atattacccc agacacccca
    35941 tcttatcgtt tgatagccct gtatccttca cgtcgcattg gcgcgaatat gctcgggctt
    36001 tgcttttcgt cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg ctgcgaaatc
    36061 gcttcagcaa acgaataaat agcaggaatt tacgccatta aattcacgac gctttaaata
    36121 agcgtaactt atggaaatgt taaaaaatcg ccatacataa caccaaaggt gtaggtcgga
    36181 taagatgcgc aagcatcgca tccgacatta ttgcggcacc ggagtttggc aacggtgccg
    36241 gatgcggcgc gagcgcctta tccggcctac gttttaatgc cagcaaaaat ggtgaattac
    36301 ctggataatc aactcacggg tgggtttaat aaaccgcgtc tccagatatt catcaggctg
    36361 atgagcttga ttgatggaac ccggaccgag caccagcgtc gggcataacg tttgaataaa
    36421 cggcgcttcg gtacagtaat tcaccacttc ggtttttgct ccgagcaatt tctcaaccac
    36481 ttcaaccagt tgatgattcg gtgggcattc atagccaggg atcggcggat gcagctcgtc
    36541 gaccgtcaga cgacccggcc agcgttcgct caccggagcc aatgcatcgt tgagcaaacc
    36601 attaagttca ttgagtgtca tgccaggctg cggacgaata tccatatgca actcacagca
    36661 agcgcaaata cggttagaag cgtcgccacc gtgaatatgc ccgaggttga gcgtagggta
    36721 tggcacggta aatgcttcgt agtgataacg ttctttcagg ttatcgcgca attgcaaaat
    36781 atgcccgatg gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg gatcgctgga
    36841 gtgccccgac tggccctgaa tacggatggc gttagagata tgacctttat gtgcgcgtac
    36901 cggttgtagt gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg cggtagtttc
    36961 ggcaaaataa cgcgctccgg ccatactggt ttcttcatca gcagtcgcca gaatgtagag
    37021 cggttttttc agtttcgtga cgtcgacatc gcgtagcgca tcaaggataa acgcaaaaaa
    37081 gcctttcatg tcggcggtgc ctaagccgta aagcttgccg tcatgctccg tcagtgtaaa
    37141 cggatcgcgc gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc ccgccagcaa
    37201 caagccgcca gccccctgtc cgtaactggc cagcatatta aatttgttgc gagttcctgg
    37261 aacaggctgc acttccacat tgaagcccaa atctttaaac cagtccgcca gcagagtgat
    37321 taaatctgca ttgctttgat cgagtgcctc ttccgtagcg cttattgaag gtgtggcaat
    37381 cagagcgcgg taaatctcga taaatggcgg taatttgttt ttcattgttg acacacctct
    37441 ggtcatgata gtatcaatat tcatgcagta tttatgaata aaaatacact aacgttgagc
    37501 gtaataaaac ccaccagccg taaggtgaat gttttacgtt taacctggca accagacata
    37561 agaaggtgaa tagccccgat gttgaatacg ctgattgtgg gtgccagcgg ctacgctggc
    37621 gcagagctag tgacctatgt aaatcgccat ccgcatatga acataaccgc tttgactgtt
    37681 tcagcgcaaa gcaatgatgc gggaaagtta atctccgatt tgcatccgca gctaaaaggc
    37741 atcgttgagc tgccgttgca gccgatgtcg gatatcagcg agtttagccc aggggtggac
    37801 gtagtgtttc tcgccaccgc ccatgaagtt agccacgatt tagcgccgca gtttcttgaa
    37861 gcgggctgcg tggtgttcga cctttccggc gcgtttcgtg ttaacgacgc caccttctat
    37921 gaaaaatatt acggctttac ccatcaatac ccggaactgc tggaacaggc agcctacggt
    37981 ctggcggagt ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt gccaggctgt
    38041 tatccgacgg cggcacagct ggcgctgaaa ccgttgattg atgccgatct tcttgacctc
    38101 aatcagtggc cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg taaggcagcg
    38161 atttcaaata gtttttgtga agttagcctg caaccgtatg gcgtctttac tcatcgccat
    38221 caaccagaga tcgccacaca cctcggtgct gacgttatct tcaccccaca tctgggcaat
    38281 ttcccgcgcg gcattctcga aaccattacc tgccgcctga aatcgggtgt gacccaggcg
    38341 caagtcgcgc aagcgttaca gcaggcgtat gcccataaac cgctggtgcg gctgtatgac
    38401 aaaggcgttc cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat cgggtttgcc
    38461 gttcagggcg agcatctgat tattgtggcg accgaagaca acttactgaa aggcgcggcg
    38521 gcacaagcgg tacagtgcgc caatattcgt ttcggctatg cggaaacgca gtctcttatt
    38581 taagggtgca atgatgaatc cattaattat caaactgggc ggcgtactgc tggatagtga
    38641 agaggcgctg gaacgtctgt ttagcgcatt ggtgaattat cgtgagtcac atcagcgtcc
    38701 gctggtgatt gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag ggctgaatct
    38761 gccggtgaaa aagaaaaacg gcctgcgggt gacgcctgct gatcagatag acattatcac
    38821 cggagcactg gcgggaacgg caaataaaac cctgttggca tgggcgaaga aacatcagat
    38881 tgcggccgta ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc agcttgatga
    38941 agagttaggt catgttggac tggcgcagcc aggttcgcct aagcttatca actccttgct
    39001 ggagaacggt tatctgccgg tggtcagctc cattggcgta acagacgaag ggcaactgat
    39061 gaacgtcaat gccgaccagg cggcaacggc gctggcggca acgctgggcg cggatctgat
    39121 tttgctctcc gacgtcagcg gcattctcga cggcaaaggg caacgcattg ccgaaatgac
    39181 cgccgcgaaa gcagaacaac tgattgagca gggcattatt actgacggca tgatagtgaa
    39241 agtgaacgcg gcgctggatg cggcccgcac gctgggccgt ccggtagata tcgcttcttg
    39301 gcgttatgcg gagcagcttc cggcactgtt taacggtatg ccgatgggta cgcggatttt
    39361 agcttaagtt ttgttagccg gaggagcagc tttccggcat tgaatttcaa aataaggaaa
    39421 cagagttatg gcactttggg gcgggcgttt tacccaggca gcagatcaac ggttcaaaca
    39481 attcaacgac tcactgcgct ttgattaccg tctggcggag caggatattg ttggctctgt
    39541 ggcctggtcc aaagccttgg taacggtcgg cgtgttaacc gcagaagagc aggcgcaact
    39601 ggaagaggcg ctgaacgtgt tgctggaaga tgttcgcgcc aggccacaac aaatccttga
    39661 aagcgacgcc gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca aagtgggcca
    39721 gttaggcaaa aagctgcata ccgggcgtag ccgtaatgat caggtagcga ctgacctgaa
    39781 actgtggtgc aaagataccg ttagcgagtt actgacggct aaccggcagc tgcaatcggc
    39841 gctggtggaa accgcacaaa acaatcagga cgcggtaatg ccaggttaca ctcacctgca
    39901 acgcgcccag ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga tgctggcgcg
    39961 tgatgaaagc cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat taggctgtgg
    40021 cgcgctggcg ggaacggcct atgaaatcga ccgtgaacag ttagcaggct ggctgggctt
    40081 tgcttcggcg acccgtaaca gtctggacag tgtttctgac cgtgaccatg tgttggaact
    40141 gctttctgct gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag atctgatttt
    40201 ctttaacacc ggcgaagcgg ggtttgtgga gctttcggac cgcgtgactt ccggttcatc
    40261 attaatgccg cagaagaaaa acccggatgc gctggagctg attcgcggta aatgcggccg
    40321 ggtgcagggt gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc tggcttacaa
    40381 caaagatatg caggaagaca aagaaggtct gttcgacgcg ctcgatacct ggctggactg
    40441 cctgcatatg gcggcgctgg tactggacgg cattcaggtg aaacgtccgc gttgtcagga
    40501 agcggcgcag caggggtatg cgaacgccac tgaactggcg gattacctgg tggcgaaagg
    40561 cgtacctttc cgcgaggcgc accatattgt gggtgaagcg gtggtggaag ccattcgtca
    40621 gggcaaaccg ctggaagatc tgccactcag tgagttgcag aaattcagtc aggtgattgg
    40681 cgaagatgtc tatccgattc tgtcgctaca atcgtgcctc gaaaagcgtg cggcaaaagg
    40741 cggcgtctca ccgcagcagg tggcgcaggc gattgctttt gcgcaggctc ggttagagta
    40801 atttgtttat attgctcccc tgaaaatggg gagtttacca cgcctacaaa gtgcgacact
    40861 tcgtaggcgg ataaggcgtt tacgccgcat caggcagaaa tttgtcagca ttctggtgta
    40921 atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat ccggaaacgc
    40981 tcacttgttt ccggattttt ttatgcacat tggacaggct cttgatctgg tatcccgtta
    41041 cgattctctg cgtaacccac tgacttctct gggggattac ctcgaccccg aactcatctc
    41101 tcgttgcctt gccgaatcag gtactgtaac gctacgcaag cgccgtcttc ccctcgaaat
//
LOCUS       DAJTYI010000019        40477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYI010000019 DAJTYI010000000
VERSION     DAJTYI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            100..1533
                     /gene="cscA"
                     /locus_tag="OO564_001017"
     CDS             100..1533
                     /gene="cscA"
                     /locus_tag="OO564_001017"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6652234.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1541..2599)
                     /locus_tag="OO564_001018"
     CDS             complement(1541..2599)
                     /locus_tag="OO564_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6652235.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2767..2985
                     /locus_tag="OO564_001019"
                     /pseudo
     CDS             2767..2985
                     /locus_tag="OO564_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3003..4331
                     /gene="dsdA"
                     /locus_tag="OO564_001020"
     CDS             3003..4331
                     /gene="dsdA"
                     /locus_tag="OO564_001020"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6652236.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4439..5977)
                     /gene="emrY"
                     /locus_tag="OO564_001021"
     CDS             complement(4439..5977)
                     /gene="emrY"
                     /locus_tag="OO564_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6652237.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5977..7140)
                     /gene="emrK"
                     /locus_tag="OO564_001022"
     CDS             complement(5977..7140)
                     /gene="emrK"
                     /locus_tag="OO564_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6652238.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7556..8170
                     /gene="evgA"
                     /locus_tag="OO564_001023"
     CDS             7556..8170
                     /gene="evgA"
                     /locus_tag="OO564_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6652239.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8175..11768
                     /gene="evgS"
                     /locus_tag="OO564_001024"
     CDS             8175..11768
                     /gene="evgS"
                     /locus_tag="OO564_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6652240.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11824..12969)
                     /gene="yfdE"
                     /locus_tag="OO564_001025"
     CDS             complement(11824..12969)
                     /gene="yfdE"
                     /locus_tag="OO564_001025"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6652241.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13043..13987)
                     /gene="yfdV"
                     /locus_tag="OO564_001026"
     CDS             complement(13043..13987)
                     /gene="yfdV"
                     /locus_tag="OO564_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6652242.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14057..15751)
                     /gene="oxc"
                     /locus_tag="OO564_001027"
     CDS             complement(14057..15751)
                     /gene="oxc"
                     /locus_tag="OO564_001027"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6652243.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15805..17055)
                     /gene="frc"
                     /locus_tag="OO564_001028"
     CDS             complement(15805..17055)
                     /gene="frc"
                     /locus_tag="OO564_001028"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6652244.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17568..18191)
                     /locus_tag="OO564_001029"
     CDS             complement(17568..18191)
                     /locus_tag="OO564_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6652245.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18488..18763
                     /locus_tag="OO564_001030"
     CDS             18488..18763
                     /locus_tag="OO564_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652246.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18840..19082)
                     /locus_tag="OO564_001031"
     CDS             complement(18840..19082)
                     /locus_tag="OO564_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6652247.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19435..20355
                     /gene="lpxP"
                     /locus_tag="OO564_001032"
     CDS             19435..20355
                     /gene="lpxP"
                     /locus_tag="OO564_001032"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6652248.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20710..20781
                     /gene="ypdK"
                     /locus_tag="OO564_001033"
     CDS             20710..20781
                     /gene="ypdK"
                     /locus_tag="OO564_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6652249.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20846..22084)
                     /gene="alaC"
                     /locus_tag="OO564_001034"
     CDS             complement(20846..22084)
                     /gene="alaC"
                     /locus_tag="OO564_001034"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6652250.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22461..24158
                     /gene="ypdA"
                     /locus_tag="OO564_001035"
     CDS             22461..24158
                     /gene="ypdA"
                     /locus_tag="OO564_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6652251.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24173..24906
                     /gene="ypdB"
                     /locus_tag="OO564_001036"
                     /pseudo
     CDS             24173..24906
                     /gene="ypdB"
                     /locus_tag="OO564_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            24919..25776
                     /locus_tag="OO564_001037"
     CDS             24919..25776
                     /locus_tag="OO564_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6652252.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25779..28280)
                     /gene="ptsP"
                     /locus_tag="OO564_001038"
     CDS             complement(25779..28280)
                     /gene="ptsP"
                     /locus_tag="OO564_001038"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6652253.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28305..29342)
                     /gene="ypdE"
                     /locus_tag="OO564_001039"
     CDS             complement(28305..29342)
                     /gene="ypdE"
                     /locus_tag="OO564_001039"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6652254.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29342..30425)
                     /gene="ypdF"
                     /locus_tag="OO564_001040"
                     /pseudo
     CDS             complement(29342..30425)
                     /gene="ypdF"
                     /locus_tag="OO564_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30440..31687)
                     /locus_tag="OO564_001041"
     CDS             complement(30440..31687)
                     /locus_tag="OO564_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6652255.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31709..32035)
                     /locus_tag="OO564_001042"
     CDS             complement(31709..32035)
                     /locus_tag="OO564_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6652256.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32254..33219)
                     /gene="glk"
                     /locus_tag="OO564_001043"
     CDS             complement(32254..33219)
                     /gene="glk"
                     /locus_tag="OO564_001043"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6652257.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33423..34679
                     /locus_tag="OO564_001044"
     CDS             33423..34679
                     /locus_tag="OO564_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6652258.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34794..35120
                     /locus_tag="OO564_001045"
     CDS             34794..35120
                     /locus_tag="OO564_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6652259.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35261..36499)
                     /locus_tag="OO564_001046"
     CDS             complement(35261..36499)
                     /locus_tag="OO564_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6652260.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36835..38037
                     /gene="nupC"
                     /locus_tag="OO564_001047"
     CDS             36835..38037
                     /gene="nupC"
                     /locus_tag="OO564_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6652261.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38087..40276)
                     /locus_tag="OO564_001048"
                     /pseudo
     CDS             complement(38087..40276)
                     /locus_tag="OO564_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10759 a        10073 c         9389 g        10256 t
ORIGIN      
        1 catttgcgat attcatcgca tttatgaaag taagggacta tatttttata aaagttaacg
       61 ttaataattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc tcgattgcat
      121 gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt ctatccccat
      181 tttcacctcg cgcctcctgc cggatggatg aacgatccaa acggcctgat ctggtttaac
      241 gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg accaatgcac
      301 tggggacatg ccaccagtga cgatatgatc cactggcagc atgagcctat tgcgctagcg
      361 ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga tgacaatggt
      421 gtcctctcac ttatctacac cggacacgtc tggctcgatg gtgcaggtaa tgacgatgca
      481 attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga gaaacagggt
      541 gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt gtggcgtgaa
      601 gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg gcagatcctg
      661 ctttatcgcg gcagttcatt gcgtgaatgg gccttcgatc gcgtactggc ccacgctgat
      721 gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga tcagcattat
      781 ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa tcgctttcaa
      841 agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc cgggcatttt
      901 actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc gaaggatggt
      961 cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc aaaacgtgaa
     1021 ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg caaacttcta
     1081 caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt ctctccccgc
     1141 acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat tcagttgcag
     1201 tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac tggaatgcgg
     1261 ctgtatattg ataaccaatc tgagcgactt attttgtggc ggtattaccc acacgagaat
     1321 ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct aaggatattt
     1381 atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat gagtagtcga
     1441 atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg agtggctgtg
     1501 ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa caacggatca
     1561 acagcgggca agggatccgc gtcactcttc ccccttcacg accttcaata atatgcaatg
     1621 cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc ggtaaaaaca
     1681 gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc gggatacgta
     1741 cccccttcgc caaaagaacc tgataagcca caaaggctgc gcgatcgtta ccacatatca
     1801 gaacatcaaa atctggtttg cccgatttga agtgagcatt gagtaaactt gcgagatcgg
     1861 tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga tctcgtccag
     1921 catcacgcca agcctgctca aatccctgcc gacgataccc tgttgccaac gcactttccg
     1981 gtagccagaa gcataatggt tgacgatagc ccgccgcgag caaatgctgt gttgattcat
     2041 attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc gccacacagt
     2101 tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgg cgcagcccca
     2161 ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc gcgcgggcag
     2221 cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg ctggcggttt
     2281 gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc tgcgccagca
     2341 cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa agatcaggaa
     2401 cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga cgcacagatt
     2461 ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccaag cgtgcgacat
     2521 cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag cagaaacaga
     2581 cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa aaaataacaa
     2641 taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt aaccatatag
     2701 gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat ataaatatgg
     2761 atcattgtgc tgccactgta tcccgacatc agcccggaaa ttattgcgat tgctatcggt
     2821 tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctggt gaagcaatat
     2881 tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt tatcgcttca
     2941 gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca aagagacgta
     3001 ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta aaggatctgg
     3061 ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct gaaggtttac
     3121 cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta tcccgttttg
     3181 caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt attgaatcag
     3241 aactggttgc cattccggct atgcaaaaac ggctggaaaa ggaatatcaa caaccgatca
     3301 gcgggcaact cctactgaaa aaagatagcc atttgcccat ttccggctct ataaaagcac
     3361 gcggcgggat ttatgaagtc ctggcacatg cagaaaaact ggctctggaa gcggggttgc
     3421 tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag ttctttagcc
     3481 aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc attatgagcg
     3541 cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca tggaaaaaag
     3601 cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat ggtgttgccg
     3661 tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt gatgacgaaa
     3721 attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa gcgcaatttg
     3781 cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg ccgtgtggtg
     3841 ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc gatcatgttc
     3901 actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc catacaggat
     3961 tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca gcggatggcc
     4021 ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg ctggatggct
     4081 tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg caggaagaag
     4141 gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc gtgtgtgcat
     4201 cagtaagtta ccaacagatg cacggtttca gcgcagaaca attgcgtaat gccactcatc
     4261 tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa tatctggcaa
     4321 aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg agcgatgctg
     4381 ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc ttttatcatc
     4441 acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga
     4501 taaaagcaat agccgccatg cggaaaatct catttgcaga aatagaaagt gactgttggg
     4561 ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat ttatccataa
     4621 tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc aactgactat
     4681 ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca actgatcctg
     4741 acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta tctggcaagc
     4801 ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat ccctggaaaa
     4861 actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt
     4921 aatagcaaac cgcatacatc aaaaaactga atgtcactaa cacccgcatg tctattttat
     4981 tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg ggcgcataag
     5041 caagcccggc ccatatcgca ttatacccca tcgttttctg gagtaactgc ggcataagga
     5101 cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca atggtgaaat
     5161 tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgat tcccaaatga
     5221 ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta ctcgagttga
     5281 accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca ccgagcacta
     5341 acagggtcag tcttggtaga ttcattttta ccggtgaagt ttcagtttct cttcctttaa
     5401 gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt aaaaatatcc
     5461 aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat atcggagcga
     5521 taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt ggataattcc
     5581 ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc tgaacgactc
     5641 taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa gacagcgaaa
     5701 aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc agcctgccgg
     5761 taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag gtgccttcgt
     5821 ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa atagtggaat
     5881 ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat aacgtcccac
     5941 cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat tatgcgaaat
     6001 aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct tactggtata
     6061 agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt cttcgtcctt
     6121 cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca tgagttcttt
     6181 tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc
     6241 attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc ctgtcacccg
     6301 accatgaaac acaacatttt caccataaag atcgctgata atattgactg attgaccaat
     6361 ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt gacgtgccgg
     6421 tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa cacttcgctg
     6481 ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca accaggcttc
     6541 tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg tgttcattac
     6601 taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt tgctacttat
     6661 taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct tcgctaaagg
     6721 cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg aagcgacttc
     6781 ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa tatttgccag
     6841 attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat
     6901 gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac tacctgagac
     6961 ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac taatcatgtc
     7021 ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata aaactatcac
     7081 caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa tctgttccac
     7141 tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag tattcattct
     7201 tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag gcctaattta
     7261 aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata caattcttac
     7321 gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat catcggtata
     7381 atccctgatt ttattgttga catttcattt atgccgacta tttatatggt atacttgtcg
     7441 aattatctta aaggaagctc agattttctt atttttattg agaaaatgag atgacgcctt
     7501 atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat aatctatgaa
     7561 cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa
     7621 aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc agcgggtgga
     7681 aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca
     7741 ggtgttagaa acgctgagga agcgccaata tagtggaatt attattatcg tctccgctaa
     7801 aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt tcgtgagtaa
     7861 aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg gctactgcta
     7921 tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga
     7981 ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa
     8041 tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata aaagtcgcct
     8101 gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc aacgtaacaa
     8161 aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt ggtctttggt
     8221 cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt agtaacaacc
     8281 gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta gcgagcaaaa
     8341 aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat accgattcgc
     8401 agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata
     8461 tcaaattaac actccgggaa tacgcagatc atcaaaaagc aatggacgcg cttgcagaag
     8521 gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat aatgacattg
     8581 ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt cacgactcaa
     8641 tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat taccccccag
     8701 acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca aatttatatc
     8761 aggcattagc atccgtctca gctgggcaca atgattactt tattggtagt aacatcatta
     8821 ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa tattataact
     8881 cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt aatgaagtac
     8941 tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca caaaattggc
     9001 ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa catgaaaaac
     9061 agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg cccccctatt
     9121 ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt aatattatta
     9181 ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc catgctggaa
     9241 cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt gaagatcgag
     9301 aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt gtcatgcaaa
     9361 aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt ccatattatt
     9421 atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata caggtcgata
     9481 atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg gtcgcgacac
     9541 agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat cattttctta
     9601 ttcctggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta
     9661 aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg cgcctgacgg
     9721 aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat agcgagcaat
     9781 tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta tggggattct
     9841 acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat
     9901 cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta aactggcaag
     9961 gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat tactacaaaa
    10021 atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt tctaatgcgc
    10081 atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta tttgaaattg
    10141 ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat cttcctgcaa
    10201 gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt gatctaatta
    10261 atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa agtcagtttc
    10321 tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc ttcctggaac
    10381 ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca cttgcctacg
    10441 ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat
    10501 cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc cagaacactt
    10561 gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc agcagtacat
    10621 ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt
    10681 tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg acctccctgg
    10741 gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct ggaagtggat
    10801 tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt gcaggtcgtc
    10861 agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa aatatgcagg
    10921 gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc acaatcccgg
    10981 tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc atcacactac
    11041 ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta ttactcaaac
    11101 gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg caagcgctac
    11161 acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg ccgaatatgg
    11221 atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc atctgggggc
    11281 ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc atgaacttat
    11341 gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag ttacaccagg
    11401 ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa aataatacgg
    11461 cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa acgcataaag
    11521 atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc catcagtgta
    11581 ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt aatattagcc
    11641 atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt cagctgctga
    11701 actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc tgtcagaaaa
    11761 atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca gcacatctta
    11821 catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc
    11881 cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg gcatcattat
    11941 tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa tggcctcagc
    12001 cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta accaaacttc
    12061 agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct ggttttgtac
    12121 gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct ccagtgcctg
    12181 gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct tatcctgagt
    12241 atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt gtggtgactt
    12301 ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat
    12361 cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg cactcactat
    12421 tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa cacgcactgg
    12481 cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa tgatggtatc
    12541 gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg aagcatatat
    12601 gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt ccattgtacc
    12661 taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa atatactttt
    12721 atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa taaaactgta
    12781 atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac catgacctgg
    12841 tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc cgaaaggtcc
    12901 attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt tgctttcatt
    12961 atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt cggcatttat
    13021 gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc cacaagggag
    13081 ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta cgggtgtaga
    13141 cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg cctgccagta
    13201 ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga agcagcgcca
    13261 gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg aactcgaatt
    13321 tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta gccttcgcaa
    13381 tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc accaggatcg
    13441 ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat gcgctcagat
    13501 tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga ccaataggaa
    13561 tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt gatacggaat
    13621 caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt gaacccgcaa
    13681 ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag caacaaaacc
    13741 aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga cgagtgtccg
    13801 caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca ggaagagcgt
    13861 agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt tctcgtctgc
    13921 cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca ataaaaaatg
    13981 ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag ttggcaagag
    14041 gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg gtaatatggc
    14101 cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt ttgcgcgact
    14161 gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta tagccaacgc
    14221 cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc gttggtgatg
    14281 gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca ttattaaaaa
    14341 taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc ccactgaaac
    14401 caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg ctagcaccga
    14461 tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga cgacgacgtg
    14521 gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct tcattaacta
    14581 aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca ttaaagtaat
    14641 ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt tgctgcttgt
    14701 ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt ttcagttctg
    14761 ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca atggggcggt
    14821 tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct gccgcccatc
    14881 cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg acaacgtcag
    14941 catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta tcttcaagga
    15001 tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata aattcacgaa
    15061 gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat ggccgttcag
    15121 cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat aatgctggcg
    15181 acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc gtcgcggcca
    15241 ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca gagacccgga
    15301 tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat gctgctttgg
    15361 catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc tgtaggtcga
    15421 cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt accgttgcgt
    15481 tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg cagatccccg
    15541 gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc tcatgacgaa
    15601 aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc acaggaatac
    15661 ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg atgatatgca
    15721 taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt tttataaatt
    15781 gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg cagcaatttc
    15841 atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta atagcggcgc
    15901 agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg tcagatattt
    15961 tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag agggatcaag
    16021 tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag tcaaataggc
    16081 caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa aaatatcgaa
    16141 aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc attctggttt
    16201 gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat aaatataggc
    16261 gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac cgccaccacc
    16321 tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact gcgggtattc
    16381 ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc ggcaaaggtt
    16441 caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt tttcgcgatg
    16501 cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt cacccaacgc
    16561 tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg cgccacccgc
    16621 tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact catcaaaccc
    16681 tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc aggtgaagcc
    16741 catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt cgcggatcag
    16801 cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag aacgtttgtt
    16861 actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca gttggtgacg
    16921 cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa accaggccag
    16981 catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt taattccttg
    17041 aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat ttagcgttta
    17101 tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg gcactcgata
    17161 tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca attcctttct
    17221 gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg tggtaatcag
    17281 agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg gtgatttaca
    17341 agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa taaatgattt
    17401 gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg tttatagaac
    17461 cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata aaaaattgtc
    17521 ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta gttcacaaac
    17581 agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag gtttgcctcg
    17641 tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg agtctgttta
    17701 agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag ctcttccatt
    17761 gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc ggcttctttt
    17821 tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac aatatattgg
    17881 tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc ggtactgtca
    17941 tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg atcgccatga
    18001 aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg ttcagagata
    18061 cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc taccggtgca
    18121 ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt agccataatt
    18181 aaacgtttca tacttacctc attctttaac agttttagaa aacgcctcca tcatattggg
    18241 tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat cttacaattg
    18301 tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc tcctgtcgca
    18361 gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt aaatattgat
    18421 taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat tttcaggata
    18481 actttctatg aaagtaaact taatactttt cagcttattt ttattggtct ctattatggc
    18541 atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct ataaagagac
    18601 tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat cacaggcgat
    18661 tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa tcacgcctac
    18721 ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt cacatggcag
    18781 gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat cctgacccac
    18841 tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg ggcaagaaac
    18901 ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa gtaataaatg
    18961 agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac gttcagtacc
    19021 tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca taaataaatc
    19081 atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta atagtaagac
    19141 aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt taaatgcaac
    19201 gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc tgaaagcagt
    19261 cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt cctgactagt
    19321 ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg
    19381 ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt aactatgttt
    19441 ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac atggtttggg
    19501 cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt tctcggcacg
    19561 cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac
    19621 ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat tgccgaaaac
    19681 tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg gcccgacagt
    19741 cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa
    19801 aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg cggccgcgtg
    19861 atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca gctgatggaa
    19921 tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag aaataatctg
    19981 cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc cgatcaggat
    20041 tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc
    20101 aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt aacgatggta
    20161 agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga aggctacccg
    20221 acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga gatcatgcgc
    20281 gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt gggagaatcg
    20341 tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc cataataacc
    20401 tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct aattattacc
    20461 gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag taaataagaa
    20521 aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga gcagtttagc
    20581 tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg ctttgcccgg
    20641 tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa ttacgcggag
    20701 agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt ttgggccggt
    20761 acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc catcaggctc
    20821 ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctgg
    20881 cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc tgacgaatac
    20941 ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg ccaaagccaa
    21001 tccccggcga gacacaagcc ttcgcttcgt taaggagctt cttggcaaat tccagcgatc
    21061 ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc ttcggcattt
    21121 cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg cgtttgtact
    21181 gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc gccgccacct
    21241 gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg gcactgacca
    21301 gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag cttttcgaca
    21361 gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt gacggcgctt
    21421 tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc
    21481 gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc gcggttgggt
    21541 tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga atggcgcgct
    21601 ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgcgcccctg
    21661 caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc accgtgtcac
    21721 catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca atagtgacga
    21781 tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag cgggaaatgg
    21841 cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc gggcgctggg
    21901 ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg ttacccatgc
    21961 tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtgatgt
    22021 taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag
    22081 ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct gccacaatgg
    22141 tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag aacattttta
    22201 tcaatggatt taaatgagga actccacttc aaaaacagaa aatttttcta cgctaacaag
    22261 aaaattacca ccaattttat taacattata atttcaataa atttaccagc cagaagtgca
    22321 ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt aaatttatgg
    22381 tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca tagaggttta
    22441 atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg gtcttcgatc
    22501 gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg tttcgcgaac
    22561 tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc attttctcgc
    22621 tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg gtgaacgtac
    22681 gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc atcattaccg
    22741 gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg gcgatcccct
    22801 gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg aaaatcccca
    22861 aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg ttgaccatga
    22921 ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc tctaaaatcg
    22981 gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg gtgcaaagcg
    23041 ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg gatatcgcca
    23101 acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa
    23161 ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg
    23221 cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta
    23281 cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca
    23341 caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa
    23401 cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg
    23461 tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg
    23521 aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt
    23581 ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt
    23641 tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa
    23701 tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct
    23761 ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca
    23821 gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag
    23881 gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc
    23941 gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg aatgaaatgc
    24001 cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg
    24061 ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac
    24121 gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt
    24181 catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca
    24241 cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca
    24301 gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt
    24361 gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg
    24421 gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccgta
    24481 tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca
    24541 gaccagcgta cgcctgccgc gacggtaacg cgtgagaatg acaccattaa tctggtgaaa
    24601 gatgagcgaa taatcgtcac gccaattaac gatatctatt acgccgaagc gcacgagaaa
    24661 atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt
    24721 tgcagcaaac tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg
    24781 aacaaaatac gcgaaatcga accgtggttt aataacacct acattctgcg actgaaagat
    24841 ctggattttg aagtgccggt cagccgcagc aaagtgaaag aatttcgcca gttaatgcat
    24901 ctttaatcgg gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc
    24961 gatctgttca gcggcctagt gcttaacccg caactactgg ggcgcgtctg gtttgccagc
    25021 cagcctgcct cgttaccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg
    25081 ctgcgcggcg aatacggcaa cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa
    25141 atgctgttta ttccggcacg cgcggctaat ttaccgatca acaacaaacc ggtgatgctg
    25201 ttaagcctgg tgttcgcacc gacctggctt gggttatcgt tttacgatag ccgcaccacg
    25261 tcgttgttgc atcctgctcg ccagatccag cttcccagcc tgcaacgcgg tgaaggtgaa
    25321 gcgatgctta ccgccctcac ccatcttagc cgttcgccgc tggagcagaa tatcattcag
    25381 ccactggtgt taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat
    25441 tcgcaaccgc gcggcgattt tctctatcac agcatttgta actgggttca ggataattat
    25501 gcccagccgc tcacccgcga gagcgtggcg cagtttttta atatcacgcc caatcatctg
    25561 tcaaaactgt ttgctcagca tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga
    25621 atggcgaagg cgaggatgat tttgcagaaa tatcatctgt caattcatga agtggcacag
    25681 cgttgcggtt ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttggtctg
    25741 acgccgggag agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc
    25801 agcagcgtca ggatactttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta
    25861 ttcaccagtt tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca
    25921 cccagcgtca gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt
    25981 ttcgccagcc gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg
    26041 atcgctacgc caaaaccaac gccggtggta acaatctctt cccgctgcca gacatcttct
    26101 tccagctcaa acggatgttc agtgcgcccg ttaacgccga ggttgccgca caggaactgg
    26161 atcgcctgct ctttattgct aagatcctga tcaacaaaga tattctccag cgccagcagt
    26221 gggcgaacgt cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg
    26281 cggcattcac atgcctgacg cgccagttcc cgacacgcct cgctatccag ttgacgaagc
    26341 tggcttttca ccgccggaat acgcgggcta ctcatactca gctcatccag gcccagccca
    26401 agcagtagcg gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg
    26461 ccccgctgat gcgcagtggt aactatttgc tgcaacatgc gcaggaacga tggcgtaatc
    26521 gggttatata gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc
    26581 atatcgttgg agccgatact gaagaaatcg acctcatcgc agaagtggtc gatgatgtag
    26641 cacaccgacg gcacttccac catgatccca agcgtaatcg tctctgcatg acgcaggcca
    26701 tcgcgcttaa gctcaacgat cgctttttgg atctcgcctt tcacccataa gatctgatcg
    26761 aggctgtgaa ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg
    26821 gcccgcagtt gagtgcggaa caggccagca aattccgggt aaatacgtac cgcgcgatag
    26881 ccgaggaatg ggttctcttc ctggggaaca ttcagataag aaatgctttt atcgccgccg
    26941 atgtccatcg tgcggaagat aatcggcttg tcgcccgccg ccagtagcac ctgctggtag
    27001 gcttcaaatt gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta
    27061 cggaacagcc cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg
    27121 ccgatattcg ccgcaatatc aatacgcttg ttatcacggg aataggccag ctgcgcggca
    27181 gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg
    27241 ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt
    27301 tgcccggcat aacgggcaat cgcatccagc ggcaagccac tcagtaccgg gatcgccgag
    27361 gcacgggcca gaatcagtgt atgcgaggtg cgcccggttt tctccagaat catacccgca
    27421 agatttttca aatcaaggct caaaaactgg cttggggtta aatcttcagc caccagaatg
    27481 gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt gatatgcagc
    27541 aactgttcgc tgatatcgcg aatgtcgctg acccgctcgc gcagataatc gctggcagaa
    27601 gcagatagtt tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagtccctga
    27661 tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac
    27721 aaatgagcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctgattcagt
    27781 tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg
    27841 attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg
    27901 ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc
    27961 agcggatgcg ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc
    28021 accaggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc
    28081 aggctgcaac tgtcattaaa taaggtgcca gttccaatca acgccagcga gcttttagca
    28141 tcggcctttg cgttctggcg atggttgata aaggtgattt cgctttgcca ctggctgcac
    28201 tgttctttaa gttcccgcgc cggacgagca tgtagaccgt taggcatagg caaaggacag
    28261 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
    28321 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
    28381 tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc
    28441 cccattacca atgtgggtac gccagtaccg gttaagtgca ccgctccacc gtccgtgccg
    28501 ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgccac ggtttcaatc
    28561 cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag catcggcccg
    28621 ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
    28681 tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc gccgcgtaat
    28741 cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc ggcgtcgtgc
    28801 agctcacgta gtaacgtcac cagcagatag caaccgaggc gatcatcaaa ggctttgccc
    28861 atcacccgct ggtgagggag gacctgaaaa gtggtatcga aggtgacgcg atcgcctgga
    28921 cgaattcccg cctgcatcac ttcgtcatag gtgcgcgcgc caatgtccac gcgcatggcg
    28981 ctaacgtcat tcccctgccg gtcgccgtca agcaggccta gaattttgca ctcttcacgg
    29041 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc
    29101 acgtcaatag cgccttcgcg ggagatgctg cgcaccataa atcccacttc gtccatatgg
    29161 gcgcagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga
    29221 ccatcaaatc gcacttcttt ttgcaggcga gccgcttctt ccagcaggat ctgccgcact
    29281 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
    29341 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
    29401 gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctagc aaataaatcc
    29461 ccggctccac ggtcagcaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac
    29521 gcggatcttc atggacttca atgccgatag cgtgaccagt gttatgaccg aaatagtcgc
    29581 caaatccaga ctcggtaatg acccggcgcg cggcgtcgtc aatctgctgg cagcgcacgc
    29641 cggggcgaat tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa
    29701 acagcgggtg agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat
    29761 cagcagtagc cctgatacag cgcaccaaaa tcgagagtga caaactcgcc cgctgcaaca
    29821 atcttgtcgc tggctttgcc gtgcggcagc gccccgcgcc agccgctggc gacaatggtg
    29881 tcaaaagagg ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc
    29941 tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat gctccgctcc gcaatcggca
    30001 atcccacagg cgaggcggat tatctccacc tcctctggcg ttttgatttg ccgcagcaca
    30061 tccggcgtgg cgctaaccag tttcgcattg agttcagact tccagcggtg cgcggtttcc
    30121 caactcacct gctggccttc aaaaccgagc gtttgcaact gctcatcggc aatgatttga
    30181 ttgacgatag tggtaagcgt gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca
    30241 cgggcttcca cctcaacgaa atagcgcgaa tctaccagaa tgtgcgcact ttcacggcta
    30301 ataaccacat agcctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag
    30361 gagagaagca ccgcatccag ttgttgcacc ttaagccagt tgcgcagcga agcgagtaat
    30421 gtcatctgga ttttccttct tacaggctat cgattaacaa tttgcctttg cggaacatca
    30481 tcagacgcag gaacaccacc atcagcgcag taatgattgc ccccagcgcg atccccgcca
    30541 tataaacgcc caggttagtg accagcggcc atgcccagat agcggattcc gggaaccatt
    30601 gcactgcgcc cagccagaca gctgcggttg agccgacaat cgcgccgacc atatatgacg
    30661 gaatggcggt gatggggctt tccagcgcaa acggaatcgc accctcacta atccccataa
    30721 aggccaggaa catcgcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc
    30781 cggttaaacg acggtcaata atggtcgcca gacccagacc aatcggcgga ataacgatag
    30841 cgatagaacg agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacgaaac
    30901 ctgcggcttt gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg
    30961 cgtacatcag cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga
    31021 tccagccacc aaagggggtg atgacgtagt acatcgccag catgacaaaa atggcggaaa
    31081 gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct
    31141 ggttcatcca tttcacaagg taaccggcaa cgatagaaat aatcagcgca ccaatgaagg
    31201 tggacggcac cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt
    31261 gcgtcggctg ggtagacatc aacccgccga taaaaccagc cgggaacgcc agtttgccac
    31321 cgatagagtt cgccacaaat gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc
    31381 cgaaggactg cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc
    31441 cgctattaag agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa
    31501 tcagctggct gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa
    31561 tccctgacat cacatgctgc gggagttcac cccagaagct gcttttagag gcctgcggat
    31621 tcttaactgc cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa
    31681 tggccataac aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga
    31741 tttctttgat gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt
    31801 cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc cacggaatgg atgatgatgg
    31861 tcgcttcggc gatatcctgc gccgtcaggc gattctggat gccgtccgcg ccctgggttt
    31921 caattttcac ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa
    31981 aagtgtgagc caggcccatt gggcaggcac ataaggcaat cagtttctta ctcatcgtta
    32041 attcctcgtt cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt
    32101 accagacaaa taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg
    32161 tcggccttgt gaggggacac aaagaaaaat tgaattaact gtatgattta aaatattatc
    32221 gggagagtta cctcccgata taacaggaag gatttacaga atgtgaccta aggtctggcg
    32281 taaatgtgca ccggaaccga gaagacccgg atagtcatgg acgatgagat acaccggaat
    32341 atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccggaggc
    32401 tttgaagaac tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt
    32461 cccgagattg agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa
    32521 tgcgcggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt
    32581 gagattttct ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc
    32641 agggccagaa agcacgcgct ccgccgaaac atgaccaatt tccgcacgca atatttcgag
    32701 gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcccggcaa
    32761 gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc
    32821 gtaaaccgca ataggcttac cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc
    32881 ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg
    32941 gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat
    33001 tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac
    33061 cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga
    33121 ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt
    33181 ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag
    33241 tcaaaataat tctttctcac actgtaaata cttgggggca taacaacaac gccggaaagg
    33301 caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt
    33361 gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga
    33421 acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg
    33481 ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt
    33541 tgctctggca acgactgccg ggaactctgg ggatagccca ggattccccc ctctggatca
    33601 tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc
    33661 atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc
    33721 tacctggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg
    33781 aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac
    33841 cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt
    33901 ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag
    33961 ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta
    34021 ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga cagatggaaa
    34081 tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg
    34141 ccgtatggtg cttaccacgg ttgcacgcga taatgaatca aatgaaaaat ccggtgctcg
    34201 tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga ccagtttcgc
    34261 tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg
    34321 attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct
    34381 ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc
    34441 atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt ctcggcatcg
    34501 tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca
    34561 ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag
    34621 gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat aatgtttagc
    34681 aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc
    34741 gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca
    34801 ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca aatgcaggtg
    34861 aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat tacggtaatt
    34921 actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat gaatggcgca
    34981 aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcat
    35041 atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg
    35101 gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga tgcgacgcta
    35161 gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata aggcgtttac
    35221 gccgcatccg ccaatagtgc cagatacgac gctcattcaa ctacaatccc agcgccgtcc
    35281 ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag cctgtctgtt
    35341 ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca
    35401 gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata accagaatcc
    35461 gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac
    35521 gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc cccgccagtg
    35581 tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag accgttgccg
    35641 cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag
    35701 tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga ttgacaaaac
    35761 cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg
    35821 aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg
    35881 tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca
    35941 ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag
    36001 ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagcggtttt tgcccgcgac
    36061 gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg
    36121 aaacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg
    36181 ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat
    36241 cacgaatctg ctccgccaga tttttaccgg tggcaatccc tagtttggca gagaggatct
    36301 gaattagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc
    36361 tagcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa
    36421 tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct
    36481 caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta
    36541 ttctaaacga gaatgattat caaattcatt taaatggatt gtggtgattt ctctgataga
    36601 ccaagattac gattacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag
    36661 cacatttaca taactttcag cttccataca caacatagca gaaatgtatg acagatcact
    36721 atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac
    36781 catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac
    36841 cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc
    36901 agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta
    36961 ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa
    37021 atgttcgaaa aactactcgg atttgccaac gaagggacta acttcgtctt tggtagcatg
    37081 aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct
    37141 gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt
    37201 ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc cgtcagctcc
    37261 ctgattctgg gtcagtcaga aaactttatt gcctataaag atatcctcgg caaaatctcc
    37321 cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat gtccatcgtt
    37381 ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt actgaacatg
    37441 ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc cagtgaagaa
    37501 aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac
    37561 attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg ctttatcgcc
    37621 ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg ctacagcatc
    37681 tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct
    37741 tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc
    37801 gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc
    37861 atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca
    37921 gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaaactggtt
    37981 tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac
    38041 catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa tcagctaatg
    38101 gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta tgcaatagcg
    38161 cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc gctttcgcca
    38221 gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata tctttcacaa
    38281 agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg tagttggcat
    38341 atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt
    38401 tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca agaatgaccg
    38461 cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgtccggca atattctttt
    38521 gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg tcgcaagggt
    38581 gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt
    38641 taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg tcatatttca
    38701 gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc tgggcgtaga
    38761 gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc acctgtttag
    38821 tggtctgccc ggaaaccatc tcctctctgc tatccagcgc tagcacatga tgatgtgcgc
    38881 aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc
    38941 catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg ttccagtgaa
    39001 tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt tccggcccgc
    39061 tcagcactaa cagcagttca ctacccggca attgatacaa cttttcgttt tcctgcatca
    39121 acggcagcag cgtacggcaa attgagcgga tacagtgaac gcgcatcatt aagccgtaat
    39181 gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaaactcttg cccgcctctt
    39241 gctccggcgc ttgttccaac gcacgaaagt tgggtaggag cgttaacgga tctgttagcg
    39301 cctgcaaatg ccagcggcga ttaagccatt cactgcgatg ataaatgcgc accatataga
    39361 gcaggcaaac gctaaaggaa atcagcaccg cgagaataaa tgccagcgaa tattcggttt
    39421 ccaccccttg caaaaagttc tggttgtaat tcagaaggca aagcgttgaa acagcctagg
    39481 tgagatttaa aaacggatag cgaagattac cgaccccgag ggtaaagatg atgaagaaaa
    39541 cgggtactag gtaaccggca ataaagtcgt tttcataagg tgtgcacaac agaagtagca
    39601 gcacgcttaa cgctgccagc cagcttaagg taaatgcgcg tttctctttg cccaacgacg
    39661 gagcgatatc cctgcgccac aatatctgcg caaagtgggg acttacaatc atgcgagtga
    39721 gatagtagaa aagcatgttg taaatcagca ctgcggtgaa aaggcttagt aaatcaacga
    39781 ccgtgaaaat ggcatccgca tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga
    39841 agctccccac aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga
    39901 acagacgttg ccagacatat cggctggtca atccataacg ccaacgcgtc cccagctgcc
    39961 agcgtaatat tgcgcaggca cacaacacga caaacgtctg gctgccgagc atgaccgcag
    40021 tctgtaaaaa agagagatga aagttccaca gattggtgca aaacatcccg cataaaacgg
    40081 gaacgacacc gcgccagcca aagataaaca agattgacag cattacgcac agtggcatcc
    40141 acgccagaaa gatataactg gaatggataa tggcaagtgg cgaaataaaa cgggatagct
    40201 gaataagtac cacggtgagc gtaaacgcta gtgtgaatat cttgatattt tttatcaggt
    40261 tatgctccac aaacatgagt acactactct cactgatcgg ggttttcagg cacacaaata
    40321 ataagtttat tcgcatcgcg aagcaaacag gggccagcct gttgccgatt tttcatccaa
    40381 tgatgctttc accaggcggc gctaacccgg acgtaaactg tattacaaaa gcggcaaaaa
    40441 gcagagacaa aaaacccccg ctttgcagcg agggttg
//
LOCUS       DAJTYI010000020        40031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYI010000020 DAJTYI010000000
VERSION     DAJTYI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..153)
                     /locus_tag="OO564_001049"
     CDS             complement(<1..153)
                     /locus_tag="OO564_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR6652262.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSK"
     gene            complement(279..584)
                     /locus_tag="OO564_001050"
     CDS             complement(279..584)
                     /locus_tag="OO564_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6652263.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(692..768)
                     /locus_tag="OO564_001051"
     tRNA            complement(692..768)
                     /locus_tag="OO564_001051"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:Val,seq:gac)
     gene            complement(773..849)
                     /locus_tag="OO564_001052"
     tRNA            complement(773..849)
                     /locus_tag="OO564_001052"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(813..815),aa:Val,seq:gac)
     gene            1157..2413
                     /locus_tag="OO564_001053"
     CDS             1157..2413
                     /locus_tag="OO564_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6652264.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2454..3827)
                     /gene="mdtK"
                     /locus_tag="OO564_001054"
     CDS             complement(2454..3827)
                     /gene="mdtK"
                     /locus_tag="OO564_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6652265.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            4042..4683
                     /gene="ribE"
                     /locus_tag="OO564_001055"
     CDS             4042..4683
                     /gene="ribE"
                     /locus_tag="OO564_001055"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6652266.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(4723..5871)
                     /gene="cfa"
                     /locus_tag="OO564_001056"
     CDS             complement(4723..5871)
                     /gene="cfa"
                     /locus_tag="OO564_001056"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6652267.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6162..7373)
                     /locus_tag="OO564_001057"
     CDS             complement(6162..7373)
                     /locus_tag="OO564_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6652268.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7486..8418
                     /locus_tag="OO564_001058"
     CDS             7486..8418
                     /locus_tag="OO564_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6652269.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8415..9440)
                     /gene="purR"
                     /locus_tag="OO564_001059"
     CDS             complement(8415..9440)
                     /gene="purR"
                     /locus_tag="OO564_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6652270.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            9739..9828
                     /gene="ynhF"
                     /locus_tag="OO564_001060"
     CDS             9739..9828
                     /gene="ynhF"
                     /locus_tag="OO564_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6652271.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            9994..11163
                     /locus_tag="OO564_001061"
     CDS             9994..11163
                     /locus_tag="OO564_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652272.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11309..11890)
                     /gene="sodB"
                     /locus_tag="OO564_001062"
     CDS             complement(11309..11890)
                     /gene="sodB"
                     /locus_tag="OO564_001062"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6652273.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(12018..12845)
                     /gene="mepH"
                     /locus_tag="OO564_001063"
     CDS             complement(12018..12845)
                     /gene="mepH"
                     /locus_tag="OO564_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6652274.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13180..13527
                     /gene="grxD"
                     /locus_tag="OO564_001064"
     CDS             13180..13527
                     /gene="grxD"
                     /locus_tag="OO564_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6652275.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13566..14066)
                     /gene="tnpA"
                     /locus_tag="OO564_001065"
     CDS             complement(13566..14066)
                     /gene="tnpA"
                     /locus_tag="OO564_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6652276.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            14074..15282
                     /locus_tag="OO564_001066"
     CDS             14074..15282
                     /locus_tag="OO564_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6652277.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVAKLASLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            complement(15326..19943)
                     /locus_tag="OO564_001067"
                     /pseudo
     CDS             complement(15326..19943)
                     /locus_tag="OO564_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(20036..20683)
                     /gene="rnt"
                     /locus_tag="OO564_001068"
     CDS             complement(20036..20683)
                     /gene="rnt"
                     /locus_tag="OO564_001068"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6652278.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(20786..21193)
                     /gene="gloA"
                     /locus_tag="OO564_001069"
     CDS             complement(20786..21193)
                     /gene="gloA"
                     /locus_tag="OO564_001069"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6652279.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(21274..22371)
                     /gene="nemA"
                     /locus_tag="OO564_001070"
     CDS             complement(21274..22371)
                     /gene="nemA"
                     /locus_tag="OO564_001070"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6652280.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(22408..23007)
                     /gene="nemR"
                     /locus_tag="OO564_001071"
     CDS             complement(22408..23007)
                     /gene="nemR"
                     /locus_tag="OO564_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6652281.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            23110..23349
                     /locus_tag="OO564_001072"
     CDS             23110..23349
                     /locus_tag="OO564_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6652282.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            23398..24294
                     /locus_tag="OO564_001073"
     CDS             23398..24294
                     /locus_tag="OO564_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6652283.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            24375..24896
                     /gene="sodC"
                     /locus_tag="OO564_001074"
     CDS             24375..24896
                     /gene="sodC"
                     /locus_tag="OO564_001074"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6652284.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(24897..26879)
                     /locus_tag="OO564_001075"
                     /pseudo
     CDS             complement(24897..26879)
                     /locus_tag="OO564_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(26872..27663)
                     /locus_tag="OO564_001076"
                     /pseudo
     CDS             complement(26872..27663)
                     /locus_tag="OO564_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(27666..27902)
                     /locus_tag="OO564_001077"
     CDS             complement(27666..27902)
                     /locus_tag="OO564_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6652285.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            28103..28537
                     /gene="slyA"
                     /locus_tag="OO564_001078"
     CDS             28103..28537
                     /gene="slyA"
                     /locus_tag="OO564_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6652286.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(28584..29051)
                     /gene="slyB"
                     /locus_tag="OO564_001079"
     CDS             complement(28584..29051)
                     /gene="slyB"
                     /locus_tag="OO564_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6652287.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            29325..30434
                     /gene="anmK"
                     /locus_tag="OO564_001080"
     CDS             29325..30434
                     /gene="anmK"
                     /locus_tag="OO564_001080"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6652288.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            30532..30861
                     /gene="mliC"
                     /locus_tag="OO564_001081"
     CDS             30532..30861
                     /gene="mliC"
                     /locus_tag="OO564_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6652289.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            30920..31576
                     /gene="pdxH"
                     /locus_tag="OO564_001082"
     CDS             30920..31576
                     /gene="pdxH"
                     /locus_tag="OO564_001082"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6652290.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            31705..32979
                     /gene="tyrS"
                     /locus_tag="OO564_001083"
     CDS             31705..32979
                     /gene="tyrS"
                     /locus_tag="OO564_001083"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6652291.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            33038..33907
                     /gene="pdxY"
                     /locus_tag="OO564_001084"
     CDS             33038..33907
                     /gene="pdxY"
                     /locus_tag="OO564_001084"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6652292.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(33951..34556)
                     /gene="gstA"
                     /locus_tag="OO564_001085"
     CDS             complement(33951..34556)
                     /gene="gstA"
                     /locus_tag="OO564_001085"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6652293.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(34662..36164)
                     /gene="dtpA"
                     /locus_tag="OO564_001086"
     CDS             complement(34662..36164)
                     /gene="dtpA"
                     /locus_tag="OO564_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6652294.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(36775..37410)
                     /gene="nth"
                     /locus_tag="OO564_001087"
     CDS             complement(36775..37410)
                     /gene="nth"
                     /locus_tag="OO564_001087"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6652295.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(37410..38105)
                     /gene="rsxE"
                     /locus_tag="OO564_001088"
     CDS             complement(37410..38105)
                     /gene="rsxE"
                     /locus_tag="OO564_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6652296.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(38109..38729)
                     /gene="rsxG"
                     /locus_tag="OO564_001089"
     CDS             complement(38109..38729)
                     /gene="rsxG"
                     /locus_tag="OO564_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6652297.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(38733..39791)
                     /gene="rsxD"
                     /locus_tag="OO564_001090"
     CDS             complement(38733..39791)
                     /gene="rsxD"
                     /locus_tag="OO564_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6652298.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(39792..>40031)
                     /locus_tag="OO564_001091"
     CDS             complement(39792..>40031)
                     /locus_tag="OO564_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6652299.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT        10098 a        10038 c        10413 g         9482 t
ORIGIN      
        1 ttttgagttt tcctcatcac tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa
       61 gatagaaata gaaagtggag tttgttgctg tagaagcgag aataaggtgt agatccccgt
      121 aggcccggca cccacaatag caattttttt cattgttact tacctgtacg cattctttcg
      181 aatttagtgt tatcagataa gtttggttga gatttttaga atttgagaaa tttcagacag
      241 ggatgaaaag ggcgctaacc gcccttgatt aaattctgtc aggcgagttt tgcctgattg
      301 atttgactaa gtggctcatt gacatcgaaa actttggcga caacttcctc aacgccgaga
      361 tttttcagtc gggcagtatg tttttccaga taggcaaggg cgcttttttc atcagtgaaa
      421 aggtagatcc caccggcttc gtggttcttt tcactttctg tccatacctt ccacagaaaa
      481 ccaggttcct gattaatcga ctcagcaagt ggtttaagct gctcagccat tgcgtcgcca
      541 aatgggccat taaaagcaaa atgaaattgt aacaaagttg ccatagcgat tctccttctg
      601 ataacgcata cgaagacagt gtaaatcttt taacgtattg gatacagaga agaaacagag
      661 ggaaaaagga aaagaccaga ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg
      721 acccccacca tgtcaaggtg gtgctctaac caactgagct acggacgcag gatggtgcgt
      781 tcaattggac tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
      841 tatgaacgca acgttgtagg tgacaacggg gacgaatatt agcggcagag tggaaaggtg
      901 gcaagaggca aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa
      961 ttggctatat tttgttgatt tgcaagggcg atttttattc aggatcgcat ttacatctga
     1021 tacaacccaa taattcgtgt atcagagttt aaacagagtt gtttgccacc atctcaaatc
     1081 tgtcgtggat aaatcctccg tagcctttac acttaggtag caataaaggc acacagatgc
     1141 agcaaaggaa taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca
     1201 aattgttaag accggcgagg tcattggcgc gacgcaactt actgaaggcg agttgattgt
     1261 tgaagctggc ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc
     1321 aaccggtggg atcgccaaat gcacacagta cggtaacaat ggcacgctat cggtcagcga
     1381 cggtgccatt gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct
     1441 cgctacggtt aatggccgcc atcccgaagg tgaattcagc gttgatcagg gttatgccta
     1501 cggtttgttg ctggaaaatg gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa
     1561 aatcattctc gatcaagagg gcggcctgtt ggttaatggg acaacctcag cggtcgtggt
     1621 agatgaaggt ggtgaattgt tggtgtatcc aagtggagaa gccagcaatt gtgagattaa
     1681 tcagggcggc gtttttatgc tggccgggaa agccagtgat acgttgcttg ctggtggcac
     1741 catgaataat ctcggtggtg aagactctga cactattgtt gagaatggat ccatttatcg
     1801 tctggggacg gatggccttc agctctacag ttccggtaag acgcaaaacc tgtcggtgaa
     1861 tgtgggggat cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg
     1921 aacagtgatc ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg
     1981 cgcaccggtt gaactgaccg ggagtgttgc attactggac ggcgcttcaa tgattattgg
     2041 ttatggcgca gagctgcaac aatcaacgat tactgtacag cagggcggtg tgttgattct
     2101 cgacggcagt acggtaaaag gtgacagcgt cactttcatt gttggtaaca tcaatctgaa
     2161 tggcggaaaa ctgtggctga tcactggtgc ggcaacgcat gtgcaactga aagtgaaacg
     2221 cctgcgcgga gagggagcga tttgcctgca aaccagtgcg aaagaaattt cacctgactt
     2281 catcaatgtg aaaggggaag ttaacgggga tatacgcgtt gagataacag atgccagtcg
     2341 gcaaactctg tgtaacgctc tgaaattaca gccagacgaa gacgggattg gcgcaacgct
     2401 ccagcctgcg taaaaacaca ggcgcaaccc gtgaaggttg cgccttgtct ttattagcgg
     2461 gacgctcgtt gcagaatgat ggctgacggc atacgttgca ggaaccgcat acgcagcatc
     2521 atcataatgg ctgccgatgt caggccgata ataaagccta tccagaagcc tgctggcccc
     2581 ataggttcaa cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc
     2641 cagtaagctg taaaggtgat atagaaaatg gaacgcgtat ctttataacc acgcaaaatc
     2701 ccactgccaa tcacctggat tgagtcagaa atctgatata ccgccgccag caacatcaaa
     2761 tgcgcagcca gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc
     2821 agtgaaaccg tgaaaatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc
     2881 cgcgcagcgg tttgcgcatc cagcgttgag ccctgaccca gacgataacc tacgcggata
     2941 gttaccgctg ccgccagcga cattggaagc acaaacatca gtgaactaaa gttcagggca
     3001 atctggtgtc ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca
     3061 aacagtgtca cttcaaagaa cagtgccagc gcaatcggca aaccgagttg aatcagtcgt
     3121 ttcataaccg caggatcggg cttagcggtg cctttttcgt tacgaatatc gcgcatggag
     3181 cgggcgcgtt taatgtaaga aaccatggca aggaacatga cccaatacac cgccgcagta
     3241 gccacgccac aaccaacgcc accgagctca ggcataccga aatgaccata aataaagata
     3301 tagttcaccg ggatgttcac cagcaggccg ataaagccca ttaccatacc cggcttggtt
     3361 tttgccagac cttcacattg gttacgggca acctggaaga acagatatcc cggcgcgccc
     3421 cacaacaacg cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc
     3481 atggagcgga taatgtaacc tgcattccac agcaccagca taatgagaac ggaaacaaaa
     3541 cctgccagcc agaaaccttg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca
     3601 tttaattgcg cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc
     3661 ggaagccaga tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc
     3721 gccatcacgg tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg
     3781 attgctaatg ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt
     3841 atttgtagtt atatgagaga ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag
     3901 gggaaattca gtctattgta gcgaggattt attaattctc cagtgaaaaa atagccattc
     3961 ttaccccttc actggtaagg gcgttttcca cctgctattg tgctgaacag aatgtcttaa
     4021 ctgatttcag gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg
     4081 tcgattgacg agaaaccaaa ttttcgtacg catgtggtgg agttacccga ccacatgctg
     4141 gacggcctgg aaaccggtgc ttccgtggcg cataacggtt gctgcctgac cgtgacggaa
     4201 attaacggca accatgtcag ttttgacctg atgaaagaaa cgttacgcat taccaatctt
     4261 ggcgatttaa aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa
     4321 attggcggac acttaatgtc aggtcatatt atgaccaccg ctgaagtggc gaaaatatta
     4381 acctcggaaa ataatcgcca gatctggttt aaagtccagg atagtcagtt gatgaaatat
     4441 atcctgtaca aaggatttat tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg
     4501 ccaacgcgtt tttgcgtcca tttaattccg gaaacactgg aacgcacgac tcttgggaag
     4561 aaaaaacttg gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat
     4621 acggtagaac gtgtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc
     4681 tgatctgaac tcgcccccga agagggggcg atagaatagc ctttagcgag cgactcgaag
     4741 gccgttttca acaccgcgtg agaacaccac ctgccagagc tgaatatcac gggcgcggaa
     4801 agcacctgcg caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt
     4861 atccgcaatt tctggccatg cggcgaggaa tcgttcatac cacgccatca acgtagtatc
     4921 gtaatcagca ccgaagttat gccagtcttc catcacaaag tggggttcgc tggattgagc
     4981 aatctggcgt acagagggca ggcaaccgtt cggaaaaata tatttattaa tccagggatc
     5041 aacattcaga tcggtttttt tcgaaccgat agtatggagc aggaatatac cttccggttt
     5101 caaattacga tccaccaccg caaaataggt atcgtaattt ttcggtccga cgtgctcgaa
     5161 catccccaca gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa
     5221 aatggtgaca tccaggcctt tacagcgttc ctgagccatt ttttgctgtt cggcagaaat
     5281 ggtgacgccg accacgctta cgtcatagtt agatgccatg tagtgcgcca gtccgcccca
     5341 gccgcagcca atatccagta cgcgcatccc tggttttaac tgcaattttt cacaaatcat
     5401 tttgagcttc gcctgctggg cagattccag attatcggca tctttccagt aagcgcagga
     5461 atattgcatg aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc
     5521 cttgccgact atccaggcac gttttttact ctgcagattg aagagacgag cgccggcaat
     5581 acgcagcgtg tctttgaaat gatgggggag ttggttctcg agacctgcgc gtaagacttt
     5641 gctaaaaaac atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa
     5701 ccccaaagag ccttcctgca gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc
     5761 cgccggggca gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg
     5821 gtaccagttg tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc
     5881 atcactggtc ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa
     5941 atctcgcaga aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc
     6001 cttgccgaaa tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta aaaacataat
     6061 atttatcgta gctttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca
     6121 aaattttgtt agcagcctaa gtataagtat atcgatatag atcagtgtga ttcgctatga
     6181 gcgacttcgg cattgccatg attcgggcag tcaacttctt cacaacgttg catcatgtaa
     6241 cccagcgcga ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg
     6301 ctgatactaa taagccagga aactaccaga cttgcgagga agcacagacc cagttgcaga
     6361 gtgttctgca acgctgcggc gcgaccggtt gcatgtggga agggacgcag cgcctgggcg
     6421 acaacaatag ggtagatcgc gccattggca atcgccatca cacagaatgg gatcaggatt
     6481 tcgaccagcg acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca
     6541 aacagcacca gcaaccacgg taataactgc ttgccttgcc atttctgcag tgcggtgcga
     6601 cagccataac caccaatcag aaacgcgata gtttgcggga cataacttaa accaataact
     6661 gccgggctgt agcccatttc actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa
     6721 ctggctgaac aggctgcgta tatcagcacg ttgccgcgat aggttttaga acgtagcagg
     6781 tcggtaaagg tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag
     6841 aaaataggca gaatcagcac cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag
     6901 gaaaaatgga ccagcagcca gcttcctaac aggggagcca gtgccggaga cagacccacc
     6961 agcggcatga tggtcgcaaa aatacggttg actttctgtg aaggataata atctgtcact
     7021 aatgcttgcc agataaccgc cgcggcgcag acccccacag cctgtacaaa acgcaatacc
     7081 agcagcgtgg cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc
     7141 aggccgatta ataacaccgg tttacgacca taacggtcgg agagtggccc ccacagaagc
     7201 tgggctgcgg caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc
     7261 gtttgcaggt cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa
     7321 ccgagtacgc tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt
     7381 ctctaaatct ctcaattaaa caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa
     7441 acggtaatat ttgatgactg gtttcaaaaa ttttgcaggc tgactatgtg gtcagaatat
     7501 tcactcgaag ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag
     7561 ctgcatcgcg ttccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg
     7621 gtaccgctct ttgaacgacg gcaccgtgat gtggagctga ccgctgctgg agcgtggttt
     7681 ctcaaagaag ggcgctctgt tgtcaaaaaa atgcagatca cccgccagca atgtcagcag
     7741 atagcgaacg gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagaa
     7801 cgtacacggc agatgatcgt tgatttttat cgccattttg atgatgttga acttcttgtc
     7861 tttcaggaag tgttcaacgg tgtctgggat gcgctttccg acgggcgcgt ggaactggca
     7921 attggcgcaa cacgcgcgat tccggtaggc ggtcgttatg ccttccggga tatggggatg
     7981 ctaagctgga gttgcgttgt tgccagtcac cacccgctgg cgttgatgga tggcccgttc
     8041 agcgatgata cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta
     8101 cctaaacgta ttacctggtt gctggataac caaaaaagac ttgtcgtacc tgactgggaa
     8161 tcatcggcaa cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc
     8221 aaaccgtggc ttaatgaggg gaagtgggta gcacttgaac tggaaaaccc cttcccggat
     8281 tcggcatgtt gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggctgctt
     8341 gagtatctcg gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact
     8401 cccgcaacgg gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt
     8461 tcaatcaaac gcggatgcac ttcaatggac tgcggttctt cacgtttgtt gacgatacga
     8521 tccaacagca tgttgaacgc tgtttcaccc agcgaatctt ttggctgatg gatcgtggtc
     8581 agcgccggcg taaaatagcg cgcgttgcgc acgttatcat aaccgatcag cgaaacatcc
     8641 tgcgggacgc gcaggcccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg
     8701 ccaccacaga agacggcagt agggcgatgc ggctgcgaca ggatttgctg catggcgcga
     8761 taaccggatt caggttcaaa gtcaccctgc acaatccagc tttccggcac cttgatcatc
     8821 gcttcttcca tcgccttcat aaaaccggca aggcggcctg cgccggtgtt acgttccagc
     8881 gggccgggga tgacgccgat ttcgcggtga ccgcgttcaa tcagataacg cccggccatg
     8941 tagccgcctt cgaacgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc
     9001 cagtccatca ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct
     9061 gggtactcag aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga
     9121 taagcccgct gtttctcaag attgttccac gcattaccca gaatcagggt gtaacctttc
     9181 tggaagcaat ttttttcaac tgcttcaatg atctcggcaa aataggccgc ttcgctgctg
     9241 gtcgccagca aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta
     9301 ggggagtagt gtaattcttt aatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg
     9361 aaacgtgttt tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct
     9421 acatctttta ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaaat
     9481 cgcaaggtaa acgtttgcct ttacacacct tacgcacaac aaaagtgttg cggtacgccg
     9541 gaaaaacgac acggagcgtc aggaaggggt caatggccag tacgcaaata gtgtaagcgt
     9601 ggaattttgg ccgatcttga cgaaaagggg aagtggtaaa aacacttatc attataaatg
     9661 acgggagatt ttttcataac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat
     9721 aaaaaaggag aaaacttgat gagcaccgac cttaaatttt cactggtaac aacgattatc
     9781 gtcctcggtt tgatcgtagc cgtgggtttg actgccgcgc tgcactgatt tcctctcgag
     9841 ggagcgcgtt ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc
     9901 tctttttgtt attttcattt ttttgtgatt gctgtcatgc tttcgccact ttaatgaata
     9961 tcgcgtaatg acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctggcg
    10021 ctggcgattg gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc
    10081 gtcattgcgc gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcctat
    10141 gcagttggcg taatggtggg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc
    10201 cgcagtgcgt tgattttcct gatggcaatt ttcacgctag gcaacgttct ttccgccatc
    10261 gcgccggatt atatgaccct gatgctttca cgtattttga ccagcctgaa tcacggagca
    10321 ttttttggtt tgggttcagt cgtggccgca agcgtggtgc caaaacataa acaggccagc
    10381 gcagttgcca ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg
    10441 acctggttgg gtgaaaccat cggctggcgg atgtcatttc tggcaacggc ggggctggga
    10501 gtgatttcaa tggtaagtct gttcttctca ttacctaaag gtggtacagg ggcacgacct
    10561 gaagtgaaaa aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg
    10621 acggtactgg gagctggtgc aatgtttacc ctctacacct atatctctcc ggtactgcaa
    10681 agtattaccc acgcaacacc ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga
    10741 ttctctatcg gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg
    10801 aaaggctttt tgttgctgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat
    10861 gagttcggcg cagctattag catggtggtg tggggcgcag caacctttgc ggttgtacct
    10921 ccgttacaga tgcgcgtgat gcatgtcgcc agtgaagcgc caggtctgtc ttcatcagtc
    10981 aatattggtg cctttaatct tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt
    11041 tccgctgggc tgggatacag ctttgtgccg gtgacggggg cgattgtcgc gggactggca
    11101 ttattgctgg tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt tgctaacagc
    11161 taattaaaca aaaacgcaga aggataacct tctgcgttac agttgctgac aacgtgcgat
    11221 ttatcttgcc gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat
    11281 caggcaatgc tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc
    11341 agttcaccag cgcccagaag tgctccagat agccaggacg tgcattgcga tagtcgatgt
    11401 aataagcgtg ttcccagaca tcaacggtca gcagcggagt cgcatcggtg gtcagcggag
    11461 tacccgcgtt agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg
    11521 tccagccaga accaaagttt ttgatcgctg catcagtaaa ctgcgctttg aaatcggcaa
    11581 agctgccaaa agatgcggcg atagcttcag cgacttttcc agtcggttcg ccaccggcgt
    11641 tcggtgccag gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata
    11701 cgccaccttc agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt
    11761 taatcaggtt gttcaggtta gtgacataag tctgatgatg cttgccgtag tgatactcga
    11821 tagtttccgc agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt
    11881 cgaatgacat tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg
    11941 cgtatggata gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt
    12001 aacaacaggg taaagtttta gcgaagtgtt tttggggtca ttacccgacg agcgccaaca
    12061 tagtggcgct gccagtagtc ttcactgaga gaagtgattt gaatttcctg acctgtgcgc
    12121 ggtgactgaa taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt
    12181 ccctgagtac ggaaaaagac caggtcgccg tttttcagct cactacgttc gataggtcct
    12241 gcatcacgca ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct
    12301 ttataagcgt aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga gctgccgccc
    12361 caacgatatg gcttaccaat ttgctgcatc agtttattca ttgccacttt cgtggctttt
    12421 tgcaccttcg ctttgtgcgc gtcggcaagg gttcctgacg cggcattttt gactttcaca
    12481 caatgcgatt tatagccctt acgtttggta cacttttcgg taaagctggc tgaggccgtt
    12541 ttattgacgg cgtgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt
    12601 ttaacggaag aagcggtttt gcttttggtc gtacttttac tggcggcttt tttcgctgtt
    12661 ttctggctgg ctttctgggt ctttttggtg gttgccgtgc ttttcttttt atctgctttt
    12721 ttggtgatat gagcggtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc
    12781 gttaaaggca gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc
    12841 gccactgagc gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact
    12901 tgatgattct aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt
    12961 ttcgttggcg agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat aaatacgcca
    13021 gtttgccgcg catcagagaa gaaacgaata tttgaaatgc aacaaataga gcaaagggat
    13081 aaaatagcaa aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg
    13141 tcagattact gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa
    13201 atccaacgcc agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg
    13261 ccgagctgcg gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt
    13321 gcctatgttg atattctgca gaatccggac attcgtgcgg aactgccgaa atatgctaac
    13381 tggccgacct tcccgcaact gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg
    13441 atcgaaatgt atcagcgtgg cgaactgcag cagctgatca aagaaactgc cgctaaatac
    13501 aagtctgaag agccggacgc ggaataatcc gtcctgcaat actgacggat gcgaacgggc
    13561 atccgtcagt cctttatccg gtgcgccgta aaacctcgtc cttcagggcg gggatataag
    13621 gcgcggtttt ccacctgacc aggtgtttgc tgttgttcaa tgtattggcg gatgacggat
    13681 attggcgcac cttcgcagct actggcaaaa tagccaggac tccacaaaac gcctttgtag
    13741 taatacctga ctgcaatatc tggtcgatct cgtcgcagta acctattcga tatgcctttg
    13801 aggctgttta ccagactgga tatcgccagt ttgggaggat agttaattaa caaatggacg
    13861 tgatctggtt cgccatccat ttcaaccagt tcagctgcaa aatcagcaca tacatttgaa
    13921 aagtaagtgc gtagtttttc tgtcgtgtca tggtcaaaaa tctggcgtcg gtatctggtg
    13981 acaaagacca ggtgaacatg catcaggaaa acacaatgcc ttccacgccg gatatcggtt
    14041 tcttttttca cagaccaaag tatcatttga cctgtgaaac gattacaggc atttaaattc
    14101 cagttaagac ccggtggtca acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt
    14161 ttcgttttca atcgtgctct ggcgcttcag aatgagaatc atgaggccgg aaataaatac
    14221 atcccttacg ggaaaatggc ttcctggctg gttgagtgga aaaacgccac tgaaacgcaa
    14281 tggcttaaag attcgccctc acagccattg caacagtcac tgaaagacct tgagcgggct
    14341 tacaaaaact tcttccggaa gcgggctgct tttccccgat tcaaaaagcg gggacagaat
    14401 gctgcattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg tatttttctg
    14461 ccgaaacttg gctggatgcg ctaccggaac agccgtcagg tcacgggagt tgtgaaaaat
    14521 gtcactgtca gccagtcctg cggtaagtgg tacatcagta ttcaggcaga aagtgaagta
    14581 tcaactccgg ttcacccttc agcatcaatg gtcgggctgg atgctggcgt ggctaaactt
    14641 gcctcgctgt cagatggcac agtctttgag cctgtaaaca gttttcaaaa aaaccagaag
    14701 acgctggcga gacttcagcg acagttaagc cggaaggtca aattcagcaa caactggcag
    14761 aagcagaaac gcaaaataca gcgactgcat tcctgtatcg caaatatccg cagggactac
    14821 cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg
    14881 aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc
    14941 cgggcaaaat caggtttaaa ccgttcgata ctggatcagg gctggtatga aatgcgccgc
    15001 cagcttgagt acaagcagct ctggagtggc ggtcaggtgc ttgctgttcc gccagcgtac
    15061 acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaaccgcct gtcacaaagt
    15121 aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaatgg cgctcgtaac
    15181 attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgctcg
    15241 ttgaagcagg aacccaccga aatgattcag gcgacagctt gaacgtagca gggaaccacg
    15301 tctttcaggg cgtggaggat gtcaactatc cccaatccag cccttgtggc gaacttgaaa
    15361 atcccgcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggaaga tcattcactt
    15421 ctgttagcgt aaagcgcagc cttggttcgc gacgcagtgc ggtggtcagc gcctggaaaa
    15481 tctctggggc gagtaattcc tccttttcct gccagaccag catttttttg ccgccttgcg
    15541 gcagatagag taacaatttg ccgccagaaa cgaccaccag cgcacccgcc cgacgcgttg
    15601 gtaccagcgc tgccggatgt gcaggccagg gcagaagatt tccccacaca ttagccggat
    15661 cgttggcgga gagcgccact ggtgtatagt ggcgtgtttg cgtggcttgt gtcgccagat
    15721 cgcgcaatcg gtcaatagtc agacgttcag cgaattgcgc gccacccaga ccttctacaa
    15781 aacgacctcg cataattcgc ccggagtctt ccatacttcg acaaagtgtt tgcatcgatg
    15841 gaaacccgcc agggatattt tcggctatca ccgcctgacg actgatgatg ccgtagcggt
    15901 cgagcatatt ttccgccagc gccagcatcc tttcggtatc gtttagtggc tccacctgca
    15961 ataacgacca gcgtccagcc agatttggtg tgttgtaaga tacccgcggc gagacgggtt
    16021 gtgcatagac aggacgccca cgacgagccc ggtgactgcg gcgagttgag gtgcgtgcgt
    16081 tggaactgct gcgggtgagg gcgcgtaacg gtgcccaaat atcgctggta atgacgcctt
    16141 gccagaccag cgcccataac gcctcttgca gtgccgagga atcaaccgat tcgccgattt
    16201 tatcgcgtat ccgttggctg atttgttgtg caaaccaggc tcctccgtca gccagaacag
    16261 cgattatcgc ttgctgcagc gccgaacgat tcgcctgatc cgcttcggcg ggagtgaacg
    16321 attctgcggc atatttctgt agatgcagtg ccaccaggcc gtcatcttca cccagctttt
    16381 ttttgtcccg accagataac cgctccggtt gccagtaatt catcgagcat ttctggcgaa
    16441 tagtcgcgta cgcgggcagg caggatctgg ctttcccaga gtgaggcggg taaaccgact
    16501 ccggcaagct gttcgatcac ccgcatcacg ccatctacgc cctcataaac gcctggcgat
    16561 gttgaggcaa agagcgccgg gctaccatcg gtggcgggta atacgccctg acgttccagc
    16621 agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg cttctctggc ggcttgcagc
    16681 gagcgcaaac gcagacgacg aaatacttca tcgctcaccc agatgtcttg ttgcagattc
    16741 atcaccagac cctgttcacg cagttgctga agctgctctt cgacaatggc aataccgaga
    16801 ctaaactcat gagccagttg ttcagccgtg accagagcat gagcccggag atagcgcaga
    16861 aagaggtcgc gaagcgggta actgactcta tgtaaataaa tctctggcaa tgactctggt
    16921 agtcgtacgc cgagggcatc acgcagcctg gcggcatcat ccatacaggc aagacgctcc
    16981 tgtccgctaa tcatcgcagg gaagattcgt tttactgcaa gaagattttc cagatacgac
    17041 gcaacctctt cactgctgcc tgtatgccgt tgcgccaggt cttcaacggt cattggcccc
    17101 agttcgcgca gcaggtcgaa caatccttct tcacctttcg ctcttctgcc aggagccagt
    17161 cgttgcaact cttcttccac ctggcgaatg acctgcgggt cgagtaattc ccccggatcg
    17221 acctgtccga gtagattgcg cagtaactcg ctgtccagcg acagtacgga tgcccggcgc
    17281 tctgccagcg gggcgtcgct ctggtacata aattccgcga catagccgaa caataaactt
    17341 gtggcgaaag gcgagggcgt agtggtcgtt atatcggata tttgaatttc gccaccgttc
    17401 aggcgacgca tcaaacgttc cagtgcggga agatcataaa catcttgcag acattcgcgt
    17461 agggtttcga gaatgaccgg aaaatccgga tatccctgag cgatttccag caactgactg
    17521 gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg gcgtgcggcc cggcattaat
    17581 aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa gtgccgagct gcctaccgcc
    17641 tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa aaatcgcggc gtcgggcaat
    17701 ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat cactggcgac cacagacgcg
    17761 tcagcgcccc atagggcatg tattcgcccg gcaatcgcca gcgcccaggg ttcatgcacc
    17821 cgtcttccat agggagagtg caaaataata cgccagtcgc caatttcatc acggcaccgt
    17881 tcgaggacca gatggcgact ccccggaacg atgcccgtcg cgttgcgttg ctcgtcaatc
    17941 aacccctgaa tattggcgtt cgtattttct tctgccagcc acgggggaat agtcccggaa
    18001 aagaacgcgc catccgccag taaatgaaga aaatcgccga tcatctcgcc taattcagcc
    18061 ggacgtccgt taccttcacc acgccagaag gggagccggg cagaacgacc cggagcagga
    18121 gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc atgaggtagc gccgagagtg
    18181 ataatgtcgt tcacccgcga ctcatatacc atctcctcat ccagttcacc cacccgccgc
    18241 gaaccggcct tttcttcacc ttcgggtaat aacacgctat acatgccacg atccggaatg
    18301 gtgccgccgc tggtaaccgc caacaattga gcgccaggtc gggcggtcaa tatcccggtc
    18361 tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat cgccagaggg atagcgcccg
    18421 gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca gatctttcca cggtgcggca
    18481 cggcgtacgc gggagtacca ttcgtctacc tgtaatgcat ccatcgccgc ggcggcaacg
    18541 gtttgctgcg caaggacgtc gagaggatta tgcggtggtg tcaggttttc cagcctgcct
    18601 gcgaacatac actctacaat gactgcggaa tcgactaaat cacgccgggt acgggggaaa
    18661 aacagccctt tagatacacc gccaacctga tgtccggcgc gaccaatgcg ttgtaaccca
    18721 ctggcaacag aaagcggcgt tgccacctga atcaccagat ccaccgcgcc catatcaatc
    18781 cccagttcaa gactggaggt tgcgaccacg cagcgtaatt cacccgattt cagcgcctgt
    18841 tcggtgattg ctcgttgttc tttagagacg gagccgtggt gtgagcgggc aataaaaacg
    18901 tcgctacttt gtacacggtt agaggttgcg ccggaggtcg actcgaaatg ggccgcatca
    18961 acggcgatag acggggaacg ctgtaagcgt gcggcgtaaa gctcatttaa tcgtgccgtc
    19021 agtttttccg ccagcccgcg tgaattagta aagacaatgg tcgagcgatg gcgcaacact
    19081 tcatcaagga tacccgtttc aatatatggc cagatggagc cttcccggcc ggcatggctg
    19141 tcttcgccgg tgccgctggc gaccgatgag acatcatcca tattggcgac cggtacgaca
    19201 attcgtatct gcggatggcg cattgcgggc gggttgacta ccgtaaccgg gcgatcgcca
    19261 ccaagaaatg ctgccacatc gctggctgag cgcacagtgg cagaaaggcc aattcgctgt
    19321 gctgaggtgt ggagcagcgc atcgagccgc tccagactta acgccagatg cgcaccacgt
    19381 ttactgcccg ctaccgcgtg gacttcatca ataattaccg tttcgacgcc gtgtagcgtt
    19441 tcgcgcgcgc gggaggtcag catcagatag agtgattcgg gtgtggtgat cagaatatcc
    19501 ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg tatcgccagt acggatccct
    19561 acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt catcggcaat acccttcaac
    19621 aggatctgca agttgcgctg aacgtcggtg cccagggctt ttatcggtga aatatagagg
    19681 atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt cgccgccttc gcggaagagc
    19741 cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg agccagtcgg tgcaatcacc
    19801 agcgcatgtt cgcctcgcgc cgccacatgc caggtttgcg actggacagc ggtcggctgt
    19861 ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa acacgtccgg caggagcgat
    19921 gaagggtctg gattatctgc cattaacgct attccccgtg aatatcatgc ttgatgcgtg
    19981 gaatagcctc actatagatg cctgaatcat tgcatgtcat caggcatcga ctcgattaca
    20041 cctcttcggc ggcaggtagc ggccagcctc ccagacgttt ccagcggttg acgatttcac
    20101 aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga gtgcgcctgg gtgctgtcga
    20161 agtccatgcc agcggtctgg caagcctttg acaatacggt ttgtccgagt gccagcccgg
    20221 ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg gttacgtttc agcgaggcgc
    20281 gttctgcggc ggccatcata aagctgtgat caaaattggc gttgtgcgcc accataatgg
    20341 cgcggttaca gccgctcgct ttaatacctt tacgtacaac tttaaaaatt tcgtgcagcg
    20401 cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg gtcaatgccg ttgaaggcga
    20461 gggcttctgg ttgcaaattt gcgccgacaa atggttcgac gtggaaatgt aatgtggtgt
    20521 ccggcatcag ccagccttgt tcatccattt tcagggtgat ggcggcaatc tcaagcagcg
    20581 catcggtttt ggcgttaaat ccggctgttt caacatcgat cacaacagga taaaaaccac
    20641 gaaaacggtc gcacagaccg gtaagttgag cgttatcgga catctgattc tcttaatacg
    20701 gataaattgc agcgcgcatt atgacaaata ttacttgtcg atgcagtaaa gatgcgggcg
    20761 cgatgagttc acgcccggca ggagattagt tgcccagacc gcgaccggcg tctttctctt
    20821 cgattaactc aattttgtaa ccgtccggat cttccacaaa cgcgataacc gtagtaccgc
    20881 cttttaccgg acccgcctca cgggtcacgt tacccccgtt ttgacggatt ttttcgcacg
    20941 cttcagcggc gttatctacg ctaagcgcga tgtgaccata agcagtgccg agttcgtatt
    21001 tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc ttcggtttcc gggccgtagc
    21061 caacaaacgc cagtgagtat ttgtattccg ggttttcgct ggtacgcagc agtttcatgc
    21121 ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc gccaacgcgc agcatggtat
    21181 gaagaagacg catcttttta tcctcaaaat ggctaacaga ttcaaaatgt tgttttagta
    21241 tagcggcggc tttacgccgc tcgcaatgtt ggattacaac gtcgggtaat cggtatagcc
    21301 ttccgcgccg ccaccgtaga aactttcggc acgctgtggg ttaagctcag ctttgagctg
    21361 caagcgggcg accagatccg ggttcgcaat ccagtcacga ccaaatgcca ccgcatcaat
    21421 taaccctttg ccgatcagcg tttcagcttt ttctactgtg tatgcacctg cgccgataat
    21481 cggaccgtgg aaacgggcgc gtactttttc gcggaacgca tcagtatacg gttcaccccc
    21541 cgcccaatct ggttctgaca tatgcagata agcaatgccg cgtttaccca gttgttcaat
    21601 cagatacagt gcatcggctt cttcattcgg gccgttatct gtgttctgga aagtaccgat
    21661 tggcgaaacg cgaatgccaa tgcgatcggc accccattct tcaatcccgg catcgaccac
    21721 ttccagtacc aaacgtgcgc gattttccac gctgccgccg tactgatcgg tacgatggtt
    21781 tgaagaaggg gaaaggaact gatgcagcaa ataaccgtga gcagagtgga gctctaccag
    21841 atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg aaatcattga cgatacccgg
    21901 aatctcttcc agttcaagcg cacgcggcat ggatgtttca acgcggatcg cccgaccatt
    21961 ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct gaaggcgcta ccggtgcctg
    22021 accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg tgccacagct gcaaggccat
    22081 atgaccattt tcagcatgaa cgccagcggt gatttttttc catgcggcaa tttgctccgg
    22141 actatggatg ccaggcgcac ctgcatatcc ttttgcctgg gcagaaattt gcgtggcttc
    22201 actaataatc aaaccggcac tggcacgttg gcgatagtat tccgccatca acggggtagg
    22261 aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt gccataaaaa tacggtttgc
    22321 cgccgtgatc gcgcccactt tcagtgggga atacagtttt tcagatgaca taaaggctcc
    22381 cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc gtcgcaataa tgttttttac
    22441 atgggccagc gcgttttcca gtggctcgaa actgcgcgaa attttggcct gcagattcgc
    22501 gccaagccat agtgcgtaaa gcacttgtgc ctgttgtagc ggttcgccac aaaaggttaa
    22561 acaatggttc tcacggccat tttccagagc ctgcgagagc agggcgatca cgccgcgagc
    22621 acctttatcc atcgcgctgc gcatatcttc tgacagatcg cacacttcgg cagagagttt
    22681 tactgtcagg caaccactga tggttccatg ttggcaaaac tggttcagtg tttgctggta
    22741 ataagccagt atgcggtcgc ggtagttacc ttcgccggat tgcagcaact cagccagtcg
    22801 ctggtgatat gcggcgtaat gacgctcaag catggcaacg ccaaacgctt ctttagagcg
    22861 aaagtagtga tagaaggacc ctttcggcac ttcagcggtt tttagtaatt cgcttagccc
    22921 catcccggtg aatccacgtt gcaggcaaag ttgctcgccc gtcgccaaga gatgttcgcg
    22981 agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta gtagaccagt cggtctagtg
    23041 caagcaaagt tgctactttg gacattaaca gcttcaataa tggtaataat cttatctgag
    23101 gagggcgagg tggcggagca attagagttc tttcccgtcc agagcccgtg ccggggaatt
    23161 tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc gtagtcggga tgagcgtttt
    23221 aactggaata aaatgagcga tggcgaaaaa caagaggtgc tgcgcctttg tcggcagcga
    23281 ttaatgcgca aattacgcgc aaataagcca gcgtcatcag acgaaccgga gcaaccatca
    23341 ctcttttgaa cctaatcttg cgtatactcc tctcatatct gataagagga agcgattatg
    23401 gttcagcgta ttactattgc gccggaaggc ccggagtttt cccgttttgt gatgggctac
    23461 tggcgattga tggactggaa tatgtccgcc cgccagctgg tcagttttat tgaagagcat
    23521 ctggatctcg gcgtgaccac cgtggaccat gctgatattt atggtggcta tcagtgcgaa
    23581 gcggcgtttg gcgaggcact gaaactggca cctcacctgc gtgaacggat ggaaatcgtc
    23641 agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg tcattggtca ttacatcact
    23701 gaccgcaatc acatcattaa gagcgccgaa cagtcgctaa ttaatctcgc gaccgatcat
    23761 ctggatctgc tgttaatcca ccgaccagac ccgttaatgg atgccgatga agtggcggac
    23821 gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt ttggcgtatc gaactttacg
    23881 cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta cccttgccac taatcaggtg
    23941 gaaatatccc cggtgcatca gccgttactg ctggatggca cgctcgacca actacaacaa
    24001 ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg gtcgtctgtt taatgatgat
    24061 tatttccagc cgctgcgtga tgaactggct gtggtggcgg aggagttaaa cgcgggctcg
    24121 attgaacagg tggtttacgc ctgggtatta cgtttaccgt cgcagccgct gccaattatc
    24181 ggctcaggta aaattgagcg cgtacgggca gctgtcgaag cagaaacact gaaaatgacc
    24241 cgtcaacaat ggtttcgtat ccgtaaagct gcactggggt acgacgtacc gtaagcactt
    24301 ttaggaatag ccgccgttca aaaatgtgtc actggtttac acttattcag gaatgcacaa
    24361 tgaacggagg tcctatgaaa cgttttagtc tggccattct ggcgctggtt gttgcgaccg
    24421 gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt cacgtcgcaa ggggttgggc
    24481 agtcaattgg tagcgtcacc attactgaaa ctgataaagg tctggagttt tcacccgatc
    24541 tgaaagcatt accccccggt gaacatggct tccatattca tgccaaagga agctgccagc
    24601 cagccaccaa agatggcaaa gccagcgccg cggaatccgc aggcgggcat cttgatccac
    24661 aaaataccgg taaacatgaa gggccagaag gtaccgggca tttaggcgat ctgcctgcac
    24721 tggtcgtcaa taatgacggc aaagctaccg atgccgtcat cgcgcctcgt ctgaaatcac
    24781 tggatgaaat caaagacaag gcgctgatgg tccacgttgg cggcgataat atgtccgatc
    24841 aacctaaacc gctgggcggt ggcggtgaac gctatgcctg tggtgtaatt aagtaattac
    24901 gaggccagcg taccttgcgg tggtgcttgc tcaagttgcg aaagcgagca gtacagccgc
    24961 cagacaattg ccgccagctc acgggcggca ggttgatgat gacgggcaag gctgtcgcaa
    25021 atccgctgta attcttcaag tgtggccgcc agtgattttt gctgaacgcc acgctcactc
    25081 atcactcccc gcaacagtga aatacagtta tcccgtactc gcgataacgg atcggaacgc
    25141 gattcccagt cgcgcaattg ccagacaaca tgagaacagt tcagcagcac tacaccccag
    25201 cgtaataacc aacggcgagc cagcgcatcc tggctgttac tcaactgact gacgtgatga
    25261 taagtgagcg attcaaattc gctttcactc agtgttggat ggcggcttag ctgatcgaca
    25321 aaatcccggc gcagcgcgcg aatatggcgg cggcttttac gagcatccga tcctggacgc
    25381 agaatggcga acgctagcca agccagcgca acgccaacga tttttgccag attatcgtta
    25441 agaaaatcag caaaatcata caccggggga ttagtgacag cgataaacga acccataaaa
    25501 acaatcagtt gcccccacag tgcggcaaat ttgggcatct gcaatttaag aagctgcatt
    25561 gtcgccagca gtggaaagag aaacagtaaa aattgccaca gatcgctaat ctggaccatc
    25621 aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca ccagcgtgcg catcagcagt
    25681 gacaacgact taaacggtgc tgcgacggtg gagtagagta cgcagctaat tgctgccagc
    25741 gttaatgcat tggcaccggc atcccattgc gatgcaatac tccatgcgcc aatcatcatc
    25801 aacgtacaaa atgtacgcag cccgctccac atagcttcgg cgttgtcggt atgacgagcc
    25861 agagcggatg tccgtgcgag tctggcatga tcatctacac cgctttgcag acgatgtaat
    25921 tcctgactac tttgcagata aaggcggcaa aaataacgta gtcgctgcca gaaggcgacg
    25981 tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga taatacgtgc gacggtgtaa
    26041 gcatctgttt gcgaactggc gagcgccgtc agtaattgtt cgagaatttc tcgcgtggca
    26101 cctggcggtg agggccagtt gagcaacata cgtcgcaggc tggagatgac actggtcata
    26161 cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt gctggcgaaa acgatagtgg
    26221 ctccagaaag cctggatacg cagcaaattc atggtcagta tctgcccaat cacctcctca
    26281 tgtgctgcac gaatggcatc gtttgtttca ggctgccaga gtaaactggc atgttccagt
    26341 aatcgggcgt gcatgttttt caatgcagtt aaaagggcag tagcatcgga actgctaggc
    26401 aggatcatca tcatcatgcc gccgcacaaa ataccgacta tcacctcgca aacgcgcgcc
    26461 tgagcgatat cccacagctg gctggcctca gtaatattaa ccatcggaaa ggcgatgatg
    26521 gcagccgtgt agcccgccag ttgaaatgca tacgcgacgt tattcgtgaa gtgcgcacag
    26581 gcctaggtac aaaagccaag ccacgccgac atgctcaata gaaaaaacca cggctcattg
    26641 agcgtatgtc cggcaagaag cagtgccgca atggctccca gtaaactgcc agcgatgcgt
    26701 ccgaggcttt tgctgatgac accgccaacg gtgggaaagc taaccactgc agccgaggtc
    26761 atcgcccaat agggttcatc cagattcaaa taataggcaa ccgtcagcgc cagacacatg
    26821 gcaatggtat tgcgtaacgc ataacgccat tgcgccagcg tggccctgaa ccagcgcaaa
    26881 gcggacggga acgcgttggg cgagacgaac ccaggggacg ttaggtttca catccggaat
    26941 aaggcttgag tcactttcaa cgctctgatc ataaatcgcc cgaccgatac tggaaacgtg
    27001 accctgtaac gttttgttat cactatagag ggtaatttgt gctggtgcac cttcgcggat
    27061 atggcgcaac ttggtttctt caaaataacc aatgacgtaa aacgagtggc tgtcgacaag
    27121 ggcaaacagc ggtttcccgg tgtcagcgta atcgccgatg cgggtagtga ggttagtcac
    27181 ccatccactc accggggcgc gaatttccgt ttgcgccagt tgccattgtg tctgtttcag
    27241 cgtcgcctgt gcggcattaa cactggcctg catcgcttta acattgaggt tagcggtatc
    27301 gagttcttcg gcaaaaataa aattttgtga gagatgacga cgacgattcg cttcgttatt
    27361 ggcttttgcg aggtcagatt gcgctttcgc cagctgagcc tgtgcgttca gctcggcgat
    27421 ctgaaagggc gttttatcga tcgtgagcag aagttccccc gcgttaacca gttggttgtc
    27481 ttttatattc agctcaacaa tacgtcccga cacctgtggg gtgatagaaa cctgttctgc
    27541 gtgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa taattccaca gccaccatcc
    27601 ggcaagaacc gcaactatcg ctacaatgat tgttgagaaa tacttaattg ttttcattga
    27661 catagttacc acgcaatcag tattgccaga gcaaggcaaa cgcaaatcgc aaacagcgat
    27721 aaatccatta acaagggatg ccagatgtca ccggcgtaga tccagccacg aagcagacgg
    27781 tgtacgacaa gccagatgac gaatccaaac gcgaatgctt tgaaaaacgg aggaaaatag
    27841 acggacgcac cgaacaccag atcttgtaag ggcaatcctg ttgcattgag cataaacttc
    27901 acaaagtaga ttcctttacg accgaatata ttgcgttaga ttaataaata ttctttaagt
    27961 gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac ggtaaccaaa tgcagcaata
    28021 catttgtttt agcaatacaa ttgctgcaca ctattctaaa agccgcataa tatcttagca
    28081 agctaattat aaggagatga aattggaatc gccactaggt tctgatctgg cacggttggt
    28141 gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg ctggagttaa cacaaaccca
    28201 ttgggttacg ttacacaata tccatcagtt acctccagac cagtcgcaaa ttcaactggc
    28261 aaaagcgatt ggcatcgagc agccatcact ggtccgtact ctggaccaac tggaagaaaa
    28321 agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg gctaaacgta ttaaactgac
    28381 ggaaaaggca gagccactga tcagcgaaat ggaagctgtt attaacaaaa cccgcgcgga
    28441 aatattacat ggcatctccg cagaggaact ggagcaactg attacgctca tcgcaaaact
    28501 tgagcataat atcattgagt tacaggccaa agggtgaaat gaagggggcg tgtggtcagt
    28561 aacctgacca cacgcaaact tatttagcgc ggagaaacgg tcacctgact gccattgctg
    28621 gccagtacga cacgttggcc cggagagaaa cgagtgttgc cttgtttctg taccaccatg
    28681 atggtattac catcgtcttt acgaatttcc agctcgacac cctgcgtttt gttcattgca
    28741 ctctgtacgc cctgaccagc tacgccacct gcaacagcgc ctgctgcagt agccagagaa
    28801 cgcccggttc cgccaccaac agtattcccc aggaaaccac caagaacagc accgccaatt
    28861 gcaccgataa cgttggaatc atcaccgccc tgaatctgta ccggacgtac gttaacgatg
    28921 gtgccatagc tgacattctg tacttgtttc gcttcagaag cggtataaac atcccctgac
    28981 agggtgtcgt tattaacaca accgacaaga gacagaccta ccattgaaac aaccaatacg
    29041 cgtttaatca ctgaaaaatc tcctgttcac catgaaacgc cacgcaagca tccctcatgg
    29101 tcaaagtata tggcatatgt gaatccatgg ttacatgttc taccaaatat gagcaaatca
    29161 taatgaaagt aggatcaacc aattataaac aaaacattca tgatgattat tgatttgaag
    29221 ataatgtttt actaccaacg gtaaaaatcg gtcattccgc atggtattca cgaaaagttt
    29281 atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg aactatgaaa tcgggccgct
    29341 ttattggcgt tatgtcaggc accagccttg atggtgttga tgttgtgttg gcgacaattg
    29401 atgaacaccg ggtcgcacag ctggcaagct tgagctggcc gatcccggtg tctctgaaac
    29461 aggctgtact ggatatttgc cagggccagc agcttacact ttcgcagttt ggacagcttg
    29521 atactcaact cgggcgactt tttgctgatg cggtcaatgc cttgcttaag gaacaaaacc
    29581 tgcaggcgag agatatagtt gcgatcggct gtcacggtca aaccgtctgg catgaaccga
    29641 cgggcgtggc accacacact ttacagattg gcgataacaa tcaaattgtg gcacgcaccg
    29701 gaattacggt tgtcggtgat tttcgccgtc gcgatattgc cttgggagga caaggcgcac
    29761 cgctggtacc tgcgttccat catgccttgc tggctcaccc aaccgagcga cgaatggtgc
    29821 tcaatattgg cggcatcgcc aatctgtcac tgctcattcc tgggcagccg gttggcggct
    29881 acgataccgg tcctggtaac atgctgatgg atgcctggat ctggcgtcag gcaggtaaac
    29941 cttacgataa agatgccgag tgggcacggg cgggtaaagt tattctccca ctgctgcaaa
    30001 atatgctcag cgacccgtat ttctcgcaac ctgcaccgaa aagcaccgga cgcgaatact
    30061 ttaactacgg ctggctggag cgccatttac gccattttcc gggtgttgat ccccgtgatg
    30121 ttcaggccac actggcagaa ctcaccgccg tgaccatttc tgaacaagtt ttgttgagcg
    30181 gtggctgcga acgattgatg gtatgtggtg gaggtagtcg taatccgcta ctcatggcgc
    30241 gtctggcggt attactgcta ggcacagaag tcaccaccac cgatgccgtt ggcattagtg
    30301 gcgatgacat ggaagcattg gctttcgcct ggcttgcctg gcggacgctg gcgggattac
    30361 caggaaatct gccttccgtc actggcgcaa gccaggagac ggtactgggg gctattttcc
    30421 ccgccaactc gtgatatcag aattaactga attttttctt atcgtgtctg ccgttacact
    30481 tggaagatta aggagggtat ttgccctccg gaccaggaaa gtcttcggga tatgactatg
    30541 aaaaaactgt taattatcat tttgccggtc cttctctccg gttgtagcgc ctttaatcaa
    30601 ctggttgagc gaatgcaaac cgatacgctg gaataccagt gtgatgaaaa accgttgacg
    30661 gtcaaactga ataatccgcg ccaggaagtc agttttgttt acgataatca actactgcat
    30721 ctcaaacagg gcctttcagc ctctggcgcg cgttactccg acgggatcta tgttttctgg
    30781 tcgaaaggtg aggaagcgac tgtctataaa cgcgaccgca tcgtcttgaa taactgtcag
    30841 ttacaaaatc cacagcgttg agatttttcc aggggcggcg cacaatagcg tcacccactg
    30901 acaatccgta aagaaaacca tgtctgataa cgacgaattg cagcaaatcg cgcatctgcg
    30961 ccgtgaatac accaaaggcg ggttacgccg ccgcgatctt cccgccgatc cattaaccct
    31021 ttttgaacgt tggctctctc aggcttgtga agccaaactg gcggacccta ccgcgatggt
    31081 ggtcgctacc gtggatgaac atggtcagcc ttatcagcgc atcgttttac tcaaacatta
    31141 cgacgaaaaa ggcatggtgt tttacaccaa cctcggtagc cgtaaagcac atcaaattga
    31201 aaataatccg cgcgttagcc tgctgttccc gtggcatacc cttgaacgcc aggttatggt
    31261 gatcggtaaa gcagaacgac tttcgactct cgaagtgatg aaatattttc atagccgccc
    31321 gcgtgatagc cagattggtg catgggtttc gaagcagtcc agtcgcattt ctgcccgcgg
    31381 tatccttgaa agtaaattcc tggagctgaa gcagaagttt caacagggcg aagtgccatt
    31441 gccgagcttt tggggcggtt ttcgcgtcag ccttgaacag attgagttct ggcagggcgg
    31501 tgagcatcgc ctgcatgacc gctttttgta ccagcgtgaa aatgatgcgt ggaagattga
    31561 tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac tcctgattct
    31621 ggcactttat tctatgtctc tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca
    31681 gtcgttatat acatggagat tttgatggca agcagtaact tgattaaaca attgcaagag
    31741 cgggggctgg tagcccaggt gacggacgag gaagcgttag cagagcgact ggcgcaaggc
    31801 ccgatcgcgc tctattgcgg cttcgatcct accgctgaca gcttgcattt ggggcatctt
    31861 gttccattgt tatgcctgaa acgcttccag caggcgggcc acaagccggt tgcgctggta
    31921 ggcggcgcga cgggtctgat tggcgacccg agcttcaaag ctgccgagcg taagctgaac
    31981 accgaagaaa ctgttcagga gtgggtggac aaaatccgta agcaggttgc cccgttcctc
    32041 gatttcgact gtggagaaaa ctctgctatc gcggcgaaca actatgactg gttcggcaat
    32101 atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact tctccgttaa ccagatgatc
    32161 aacaaagaag cggttaagca gcgtctcaac cgtgaagatc aggggatttc gttcactgag
    32221 ttttcctaca acctgttgca gggttatgac ttcgcctgtc tgaacaaaca gtacgatgtg
    32281 gtgctgcaaa ttggtggttc tgaccagtgg ggtaacatca cttctggtat cgacctgacc
    32341 cgtcgtctgc atcagaatca ggtgtttggc ctgaccgttc cgctgatcac caaagcagac
    32401 ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt tggatccgaa gaaaaccagc
    32461 ccgtacaaat tctaccagtt ctggatcaac actgcggatg ccgacgttta ccgcttcctg
    32521 aagttcttca cctttatgag cattgaagag atcaacgccc tagaagaaga agataaaaac
    32581 agcggtaaag caccgcgcgc ccagtatgta ctggcggagc aggtgactcg tctggttcac
    32641 ggtgaagaag gtttacaggc ggcaaaacgt attaccgaat gcctgttcag cggttctttg
    32701 agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg acggcgtacc gatggttgag
    32761 atggaaaagg gcgcagacct gatgcaggcg ctggtcgatt ctgaactgca accttcccgt
    32821 ggtcaggcgc gtaaaactat cgcctccaat gccatcacca ttaacggtga aaaacagtcc
    32881 gatcctgaat acttctttaa agaagaagat cgtctgtttg gtcgttttac cttactgcgt
    32941 cgcggcaaaa agaattactg tctgatttgc tggaaataat gcattaagtg gaaaggggga
    33001 gtgagaaatc actccccctg gtttttatac agggaacatg atgaaaaata ttctcgctat
    33061 ccagtctcac gttgtttatg gtcatgcggg taacagtgcg gcagagtttc cgatgcgccg
    33121 cctgggcgcg aacgtctggc cgctgaacac cgttcaattt tctaatcaca cccaatacgg
    33181 caaatggact ggctgcgtga tgccgcccag ccatttaacc gaaattgtgc aaggcattgc
    33241 cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc
    33301 ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagcag cgaatccgca
    33361 ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa aaaggctgta tcgttgcacc
    33421 gggtgtcgca gagtttcatg tacggcacgg tttgcctgcc agcgatatca ttgcgccgaa
    33481 tctggttgag ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag aagcggttct
    33541 ggcagcgcgc gaactcattg agcaagggcc acaaattgtg ttggttaaac acctggcgcg
    33601 agctggctac agccgtgacc gttttgaaat gctgctggtc accgccgatg aagcctggca
    33661 tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac
    33721 gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca
    33781 tgtgaccgct gcagtctacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagct
    33841 gcaagtggtg gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa
    33901 gctctgaaat atctcatttc ggcccacaac gacctgtggg ccgatggcta ttactttaag
    33961 ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc
    34021 gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagaaccgta
    34081 aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt
    34141 gcctcgttca catattgcag cttcttatcc agctgcgcgc gaactgtcgg tttgtactct
    34201 tccggtgtat ctgggcgaaa cagaggtgtg aaacctttat gcagctcggt ggcgatgtaa
    34261 ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg
    34321 tcggggacgc tgtcggcaag atactgcata atcgcaacgc cttccgtcag caaagtacca
    34381 tcgtccagca gcaatgcagg cacctgcccc ttagggttaa cggaaaagta atcgtcaccg
    34441 ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca
    34501 cgcagggtaa tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt
    34561 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaagccgc
    34621 taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac agtagctttc
    34681 gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttaggcgc ggtcagcagc
    34741 atcagtactg caataacggc agtagcgaca ccaatctgca agaatacgcg accatagact
    34801 tccagtgaca tcagcgggtc ggtaacgtta tccggcacag ccatcatacc cgcaacataa
    34861 ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat gaagcccatc
    34921 agacgctgcg gaacgagttg tgcaaccatt gccagaccca gaccagagat catcagttcc
    34981 ccgatgctct gcaggccata gcttgcgacc agccagctga cagacacgat acctgcgtca
    35041 gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat caccatgccg
    35101 attgcaaact tggttggcat cggcagggta tcacccatct tgttatagat agcggccaga
    35161 atcggactac cgatgatgat ccagaacggg ttcagtgcct gatactgctc aggttctacg
    35221 gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa agaagttcag tgacgttggc
    35281 atctggctgt acagcacgaa gaagatgatt gcttcgagca tcaggatgaa ggcaacgatc
    35341 attttacgac gcgcagcacc tttcatcgcg aaagcttctt taccgaagat aaccacaata
    35401 ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag tagccaggtg
    35461 gcaacagcga tcagcgccac aacaccaata atggtcagca acaggttacg gtagttgact
    35521 ggttcaaagt ctggttttga accgtactgt ttaacccagc gttggcagaa ggcgaagtta
    35581 acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca gccgtatttc
    35641 gcggccagcc acggggtggc tatcatagag aagaaagagc cgatgttgac ggacatgtag
    35701 tacatggtga atgcaccgtc cagacgtggg tcgtttttct catagcatgt agaaagcaga
    35761 gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat acccatataa
    35821 acaataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc cagcacaata
    35881 gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca gccgccgata
    35941 gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga atccgcttca
    36001 gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc gtaataacca
    36061 aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg tttgaaagcg
    36121 ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac ctcttttttt
    36181 acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg
    36241 ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct
    36301 ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat
    36361 ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga
    36421 tgatggcttt acggagatat gttctgctat ttagcgtttt tcctagtgag atagtccagt
    36481 ttctgaaaaa tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta
    36541 aatcatttta acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc
    36601 acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg
    36661 caaacggatt acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt
    36721 taaaagagga taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat
    36781 gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt
    36841 gcgggcaatg caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa
    36901 ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc
    36961 aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc
    37021 gaatgcagtg tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag
    37081 cgcagcacga tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt
    37141 gatgatattt tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc
    37201 ttcaacgccc agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt
    37261 cgccttatta acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc
    37321 aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt
    37381 gaggatctcc agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat
    37441 gcacgttcgg cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt
    37501 cctgccagca tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa
    37561 ggggagtcgg tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca
    37621 tctgcaccgt caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac
    37681 atggcgcagg ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct
    37741 tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga
    37801 ataaaaatcc ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca
    37861 gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc
    37921 cagtgacgca gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt
    37981 cctaaaccca gagcgttagt ggcagtggac gtgaccgcca atagcggaca aaggccgagc
    38041 agttggacca gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg
    38101 ctcacgcttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg
    38161 tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg
    38221 aactggtcga aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg
    38281 attttttttc ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca
    38341 agccctggcg tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg
    38401 gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg
    38461 gcggctaccg gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac
    38521 tctggcgcag ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc
    38581 agcacctgac caaataacgc cttttgttgc agactggcct gttcagcaat tgtcgttttg
    38641 gtcatctggt tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta
    38701 atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc
    38761 ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc
    38821 acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg
    38881 ccgaacatca gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag
    38941 aatgcgccga gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct
    39001 ggtgagaaca accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga
    39061 atatgccagc gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga
    39121 tttacccatt gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc
    39181 tgttcaaccg aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga
    39241 ctaatgccat caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg
    39301 ctgaaaataa cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc
    39361 ggtaaccagc tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt
    39421 gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca
    39481 aacaccgtac ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc
    39541 aataagcctg tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt
    39601 agtttgagta cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg
    39661 agagtacccc aaccaaaaaa ccacagttgc gctgcgattc ctggcacggc tgcgagcaac
    39721 accaatagca taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt
    39781 ctgaatacca tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg
    39841 cgggcaatag ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca
    39901 ttagcctgtt gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca
    39961 gctttgcgcg gatcaacctg ttcttctggc tccgcattgg cctgttgctg ttccagcttg
    40021 cgtgctttgg c
//
LOCUS       DAJTYI010000021        37374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYI010000021 DAJTYI010000000
VERSION     DAJTYI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..82)
                     /gene="ygdR"
                     /locus_tag="OO564_001092"
     CDS             complement(<1..82)
                     /gene="ygdR"
                     /locus_tag="OO564_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6652300.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMA"
     gene            complement(220..933)
                     /locus_tag="OO564_001093"
     CDS             complement(220..933)
                     /locus_tag="OO564_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6652301.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1002..1691)
                     /gene="mutH"
                     /locus_tag="OO564_001094"
     CDS             complement(1002..1691)
                     /gene="mutH"
                     /locus_tag="OO564_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6652302.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2376..2906
                     /gene="rppH"
                     /locus_tag="OO564_001095"
     CDS             2376..2906
                     /gene="rppH"
                     /locus_tag="OO564_001095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6652303.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            2919..5165
                     /gene="ptsP"
                     /locus_tag="OO564_001096"
     CDS             2919..5165
                     /gene="ptsP"
                     /locus_tag="OO564_001096"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6652304.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5316..6191
                     /gene="lgt"
                     /locus_tag="OO564_001097"
     CDS             5316..6191
                     /gene="lgt"
                     /locus_tag="OO564_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6652305.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6198..6992
                     /gene="thyA"
                     /locus_tag="OO564_001098"
     CDS             6198..6992
                     /gene="thyA"
                     /locus_tag="OO564_001098"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6652306.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLKGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7176..7646
                     /locus_tag="OO564_001099"
                     /pseudo
     CDS             7176..7646
                     /locus_tag="OO564_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7637..8200
                     /locus_tag="OO564_001100"
     CDS             7637..8200
                     /locus_tag="OO564_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6652307.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8197..8604
                     /locus_tag="OO564_001101"
     CDS             8197..8604
                     /locus_tag="OO564_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6652308.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8589..8912
                     /locus_tag="OO564_001102"
     CDS             8589..8912
                     /locus_tag="OO564_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6652309.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            8925..12293
                     /gene="recC"
                     /locus_tag="OO564_001103"
     CDS             8925..12293
                     /gene="recC"
                     /locus_tag="OO564_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6652310.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12469..15357
                     /gene="ptrA"
                     /locus_tag="OO564_001104"
     CDS             12469..15357
                     /gene="ptrA"
                     /locus_tag="OO564_001104"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6652311.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15350..18892
                     /gene="recB"
                     /locus_tag="OO564_001105"
     CDS             15350..18892
                     /gene="recB"
                     /locus_tag="OO564_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6652312.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18892..20718
                     /gene="recD"
                     /locus_tag="OO564_001106"
     CDS             18892..20718
                     /gene="recD"
                     /locus_tag="OO564_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6652313.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20780..22111)
                     /gene="argA"
                     /locus_tag="OO564_001107"
     CDS             complement(20780..22111)
                     /gene="argA"
                     /locus_tag="OO564_001107"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6652314.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22343..23596
                     /gene="amiC"
                     /locus_tag="OO564_001108"
     CDS             22343..23596
                     /gene="amiC"
                     /locus_tag="OO564_001108"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6652315.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23671..23747)
                     /locus_tag="OO564_001109"
     tRNA            complement(23671..23747)
                     /locus_tag="OO564_001109"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23711..23713),aa:Met,seq:cat)
     gene            complement(23781..23857)
                     /locus_tag="OO564_001110"
     tRNA            complement(23781..23857)
                     /locus_tag="OO564_001110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23821..23823),aa:Met,seq:cat)
     gene            24066..25163
                     /gene="mltA"
                     /locus_tag="OO564_001111"
     CDS             24066..25163
                     /gene="mltA"
                     /locus_tag="OO564_001111"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6652316.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25402..26208
                     /gene="tcdA"
                     /locus_tag="OO564_001112"
     CDS             25402..26208
                     /gene="tcdA"
                     /locus_tag="OO564_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6652317.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26259..26702)
                     /gene="csdE"
                     /locus_tag="OO564_001113"
     CDS             complement(26259..26702)
                     /gene="csdE"
                     /locus_tag="OO564_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6652318.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26702..27907)
                     /gene="csdA"
                     /locus_tag="OO564_001114"
     CDS             complement(26702..27907)
                     /gene="csdA"
                     /locus_tag="OO564_001114"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6652319.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28099..28326
                     /locus_tag="OO564_001115"
     CDS             28099..28326
                     /locus_tag="OO564_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6652320.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28677..29594
                     /gene="gcvA"
                     /locus_tag="OO564_001116"
     CDS             28677..29594
                     /gene="gcvA"
                     /locus_tag="OO564_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6652321.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29613..30008
                     /locus_tag="OO564_001117"
     CDS             29613..30008
                     /locus_tag="OO564_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6652322.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            30001..31101
                     /gene="rlmM"
                     /locus_tag="OO564_001118"
     CDS             30001..31101
                     /gene="rlmM"
                     /locus_tag="OO564_001118"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6652323.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31145..31876)
                     /gene="fucR"
                     /locus_tag="OO564_001119"
     CDS             complement(31145..31876)
                     /gene="fucR"
                     /locus_tag="OO564_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6652324.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31934..32356)
                     /gene="fucU"
                     /locus_tag="OO564_001120"
     CDS             complement(31934..32356)
                     /gene="fucU"
                     /locus_tag="OO564_001120"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6652325.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32358..33777)
                     /gene="fucK"
                     /locus_tag="OO564_001121"
                     /pseudo
     CDS             complement(32358..33777)
                     /gene="fucK"
                     /locus_tag="OO564_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33886..35661)
                     /gene="fucI"
                     /locus_tag="OO564_001122"
     CDS             complement(33886..35661)
                     /gene="fucI"
                     /locus_tag="OO564_001122"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6652326.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35694..37010)
                     /gene="fucP"
                     /locus_tag="OO564_001123"
     CDS             complement(35694..37010)
                     /gene="fucP"
                     /locus_tag="OO564_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6652327.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8958 a         9276 c        10087 g         9053 t
ORIGIN      
        1 tcgccatgac gtaatcactg gaacacccgg aaacagcaaa cgccagtccg actgcagaaa
       61 ttattactgc ccattttttc atccggttat cctcatttgg cctacctatg ataatagtct
      121 gtttataaat cgacgttata tatcttgaaa gcaggaaaat cttaaactgt gagggtaaaa
      181 ggagtgagta cgccctcccg caggagagcg tacggagtat cagagcggat tctttttgtt
      241 gcgaatcaag ttgaggcttt caaccgcaat agagaagaac atcgcgaagt agatgtaacc
      301 tttcggtacg tggatgtcga aactttccag aatcagggta aagcccacca gaatcaggaa
      361 agagagcgcc agcattttta ccgaaggatg gcgttcgaca aaatcaccaa tcgagcgcgc
      421 ggcgaacatc atcacgccta cggcaatcac cacggctgcc atcataataa acaggtgatc
      481 tgacagaccc acagcggtaa tcaccgagtc gaggctgaag ataatatcca gcagcataat
      541 ctgcacgata gcgccgagga atgatgaaac gcgtgttttc agcccttctt cttcaccttc
      601 aatggattcg tggatttcct tgctggcttt ccagataagg aacaagccac ccagaagcag
      661 aatcaaatca cgggcagaaa tttcctgact gaatattgta aaaagcggat ttgtcaggcg
      721 cgtaacccaa gagattgatg ccagcagagc cagacgcata accatggctc ccgccaaccc
      781 cagacggcgc gcatgagcac gttgtgctgt gggaagcttt gccaccacca gagaaaggaa
      841 aataatattg tcgatcccaa gaacgatctc cagcagcgtc agcgtaccga gcgcaagcca
      901 ggcgttagga tcggttatcc atgcaaataa catctaaaaa gccctgccaa aaaaatgaag
      961 cggtaattat atgcccggaa agcggcaggt caaagcgatg gctactggat cagaaaatga
     1021 cgggccagta gtgcactggt gaaattcttc ttcaaataaa agccgcgtgg cagcgtcaga
     1081 atccgttcgc cccgggcacc aatggcttcg gtaagcgctt tcgcattcgc tgctttcggt
     1141 cttatctgta aatattcccc gtgacgagcg gtgatccgct caacctgacc gagaacaatc
     1201 atatccatta attcttccca gtcttcgcgt agctgtcggt cttcctcttc attcgggctc
     1261 cacagcaacg gtgatcctac gcgacgctgc gccagcggga tgctgcgctc gccttcaacc
     1321 ggtatccaca gtacgcgttt gagcttgtgg cgcacatggc tggtttccca ggtcaccccg
     1381 ctattacccg ttaacggggc aacacaaacg aatgttgttt ccagcggacg accaagacta
     1441 tccacaggga tagttttaag ttccacgccc agagcagcaa aatcttgctc aggtttactc
     1501 cctgcgctgg cacctagcca gatctccagt aacacgccaa tccagccctt atcgcgtttt
     1561 aaattctctg gcgtaaccag cccggcaagt gccgccagtt ctcccaatgt ataaccagaa
     1621 agttgctgtg cttgcgctaa caactgttct tcagtttcgg gaggagagag cagtgggcga
     1681 ggttgggaca tgtcatgatg ccttggttaa aaaacaaaca gcaattatgt gccgaatatt
     1741 cgcagtgtaa ctttttccac atgtgttgtt caattctctg atttatattg ctttttaggc
     1801 tgccggtaat cgagttgcgc aacactgcaa taggttttcc aaatctggtc actgaaaata
     1861 aacaggatct tacaccatgt tatccacaga aagctgggat aactgtgaaa aacctccact
     1921 actgttttca tttacagcct tgacgtgcga cgaaactcca gtttttagtg gttcagtgct
     1981 taacttagtg gcacattctg tggataaata cggcattggt tgatctttcg ccaacccgta
     2041 aatgagaggt gtgacctggg tcacgaatta ctctttttca ttactcaaat tacatgtaat
     2101 gcattgacta ataatgaata ttttaaaatg tctatatcgg gcttattcag aatgctccgg
     2161 gttttcccgt agtaattccg tagttcttca caactctatc cacagaaaag gtgaataaaa
     2221 acggctatcc accccttcct ctgtttataa ctctgcacat aactgtgagt tattcaattg
     2281 ttattagtgt gtaatgccct gcagagagtg gtttaccgtc ttcctggagt atgaaacaat
     2341 cattcgtata taaagcttta ttttgaggta gtccggtgat tgatgacgat ggctaccgcc
     2401 caaacgtagg tatcgtgatt tgtaatcgcc aggggcaggt aatgtgggcc cggcgatttg
     2461 gtcagcactc ctggcaattt ccgcaaggcg gaatcaaccc cggagaatcc gcagagcagg
     2521 cgatgtaccg tgaattgttt gaagaagtag gattaagccg caaagacgtt cgaatccttg
     2581 cttcaacgcg taactggttg cgctacaaat taccgaaacg tttggtgcgt tgggacacga
     2641 agccggtttg tatcggccaa aaacaaaaat ggtttctctt gcagctggta agcggcgatg
     2701 cagaaatcaa tatgcaaacc agcagtacac cagagtttga cggctggcga tgggtaagtt
     2761 actggtatcc ggtcagacag gtggtgtcat ttaaacgtga tgtctaccgt agggtaatga
     2821 aagagttcgc gagtgtggtg atgtcactgc aggaaaatac gccaaaacca caaaacgcat
     2881 ctgcttatcg acgtaaaaga ggttaagtca cgccaattat gctcactcgc ctgcgcgaaa
     2941 tagtcgaaaa ggtagccagc gcaccacgcc tgaatgaggc gttaaatatt ctggttaccg
     3001 acatctgtct tgcgatggat accgaggtct gttcggtcta cctggccgat catgatcgac
     3061 gttgttacta cctgatggcg acccgggggc tgaaaaaacc acgcggtcgc actgtaacgc
     3121 tcgcgtttga tgaagggatc gtcggcctgg ttggcaggct ggcggaaccg ataaaccttg
     3181 cagatgcgca aaagcacccc agcttcaaat acatcccctc cgtaaaagaa gaacgtttcc
     3241 gcgcgttttt aggcgtacca attattcaac gtcgccagtt gcttggtgta ctggtggtac
     3301 agcaacgaga gttgcgccag tatgacgaaa gtgaagaatc cttcctggtg acgcttgcca
     3361 cccagatggc agctattctt tctcagtcgc agttgactgc cctgtttggg caatatcgcc
     3421 agacgcgaat ccgcgcatta ccggcagcac ctggtgtggc gattgccgaa ggctggcagg
     3481 atgccacgtt acctttaatg gaacaggtgt atcaggcatc aacgctggat ccggctctgg
     3541 aacgcgaacg actgaccggg gcgctggaag aagcggcaaa cgagtttcgc cgctacagca
     3601 aacgctttgc cgccggagca caaaaagaaa cggcggctat tttcgatctt tactcgcacc
     3661 tgctttcgga cacccggctg cgtcgcgaat tgtttgccga ggttgataaa ggctcggtgg
     3721 cagagtgggc ggtaaaaacg gtcattgaaa aatttgccga acagtttgcc gcgctaagcg
     3781 ataactatct caaagagcgg gctggcgatt tacgtgcgct gggtcagcga ttgctgtttc
     3841 atcttgatga cgctaatcaa gggccgaacg cctggccgga acgtttcatt ctggtggcag
     3901 atgaactgtc agcgacaact cttgctgagc tgccccagga tcgcttagtc ggtgttgtcg
     3961 tgcgagatgg cgcagccaac tcccatgctg cgatcatggt acgtgcgctg gggatcccta
     4021 ccgtgatggg cgcggatatt cagccttcgg tgctgcatcg taggacgctg atcgttgatg
     4081 gctatcgcgg tgaattgctg gtcgatccgg agccggtact gctgcaagaa tatcagcggc
     4141 taattagtga agagattgag cttagccgtc tggcggaaga tgacgtcaat ttacccgcgc
     4201 agttaaaaag cggtgagcgt ataaaagtca tgctcaatgc tggtttaagc ccggaacatg
     4261 aagaaaaact gggcagccgt attgatggca tcggtcttta tcgcactgaa atcccattca
     4321 tgctgcaaag tggtttcccg tcggaagaag aacaggtggc gcagtatcag gggatgctgc
     4381 aaatgttcaa tgataaaccc gtcaccttgc gtacgctgga tgtcggagca gataagcagc
     4441 tgccttacat gccgatcagc gaagagaatc catgcctggg ttggcgtggt attcgcatta
     4501 cgctcgatca gccggagatc ttcttgatcc aggtgcgggc gatgctgcgt gctaatgccg
     4561 ctacgggcaa cctgaatatt ctgttgccga tggtcaccag cctcgatgaa gttgacgaag
     4621 cacgccgcct gattgaacgt gccggacgtg aagtcgagga gatgatcggt tacgaaattc
     4681 ccaaaccacg tatcggcatc atgctggaag tgccgtcaat ggtatttatg ctgccgcatc
     4741 tggcaaagcg ggtcgatttc atctctgttg gcaccaacga tctgactcaa tacattctgg
     4801 ccgttgatcg caacaatacc cgggtggcta acatttacga cagtcttcat cctgcaatgt
     4861 tacgagctct ggcgatgatc gcccgggaag cggaaataca tggtatcgat ctccgtttgt
     4921 gcggtgaaat ggcgggcgat cccatgtgtg tggcaatcct cattgggctt gggtatcgcc
     4981 atctgtctat gaacggacgt tctgtagcgc gggtaaaata cctgctgcgg cgcattgatt
     5041 ttgccgaagc agaaaatctt gcgcagcgta gtctggaagc gcaactggcg accgaagttc
     5101 gtcatcaggt tgcagccttt atggagcgtc gcggcatggg cgggctgatt cgcggagggt
     5161 tatagcgcgg atcatataca tatcttttaa cggtatccgg caaccagcca ggtccccttg
     5221 tgctattatt cgcacctttg gagcgcctga aacctgcggc gcgcatttca atcgctgttc
     5281 tctttcagcg aaataacaag aacttgtggt gacagatgac cagtagctat ctgcattttc
     5341 cggagtttga tccggtcatt ttctcaatag gacccgtggc gcttcactgg tacggcctga
     5401 tgtatctggt gggtttcatt tttgcaatgt ggctggcaac acgtcgggcg aatcgtccgg
     5461 gcagtggctg gaccaaaaat gaagttgaaa acttactcta tgcgggcttc ctcggcgtct
     5521 tcctcggggg acgtattggt tatgttctgt tctacaattt cccgcagttt atggccgatc
     5581 cgctgtatct gttccgtgtc tgggacggcg gcatgtcttt ccacggcggc ctgattggcg
     5641 ttatcgtggt gatgattatc ttcgcccgcc gtactaaacg ttccttcttc caggtctctg
     5701 attttatcgc accactcatt ccgtttggtc ttggtgccgg gcgtctgggc aactttatta
     5761 acagtgaatt gtggggccgc gttgacccga acttcccgtt tgccatgctg ttcccaggct
     5821 cccgtacaga agatattttg ctgctgcaaa ccaacccgca gtggcaatcc attttcgaca
     5881 cttacggtgt gctgccgcgc cacccatcac agctttacga actgctgctg gaaggtgtgg
     5941 tgctgtttat tatcctcaac ctgtatattc gtaaaccgcg cccaatggga gctgtctcag
     6001 gcttgttcct gattggctac ggcgcgtttc gcatcattgt tgagtttttc cgccagcccg
     6061 acgcgcagtt taccggtgcc tgggtgcagt acatcagcat ggggcaaatt ctttccatcc
     6121 cgatgattgt cgcgggtgtg atcatgatgg tctgggcata tcgtcgcagc ccacagcaac
     6181 acgtttcctg aggaaccatg aaacagtatt tagaactgat gcaaaaagtg ctcgacgaag
     6241 gcacacagaa aaacgaccgt accggaaccg gaacgctttc catttttggt catcagatgc
     6301 gttttaacct gcaggatgga ttcccgctgg tgacaaccaa acgttgccac ctgcgttcca
     6361 tcatccacga actgctgtgg ttcctgaagg gcgatactaa cattgcttat ctacacgaaa
     6421 acaatgtcac catctgggac gaatgggccg atgaaaatgg cgacctcggg ccagtgtatg
     6481 gtaaacagtg gcgcgcctgg ccaacgccag atggtcgtca tattgaccag attactacgg
     6541 tactgaacca gctgaaaaac gacccggatt cgcgccgcat tattgtttca gcgtggaacg
     6601 taggcgaact ggataaaatg gcgctggcac cgtgccatgc attcttccag ttctatgtgg
     6661 cagacggcaa actctcttgc cagctttatc agcgctcctg tgacgtcttc ctcggcctgc
     6721 cgttcaacat tgccagctac gcgttactgg tgcatatgat ggcgcagcag tgcgatctgg
     6781 aagtgggtga ttttgtctgg accggtggcg atacgcacct gtacagcaac catatggatc
     6841 aaactcatct gcaattaagc cgcgaaccgc gtccgctgcc gaagttgatt atcaaacgta
     6901 aacccgaatc catcttcgac taccgtttcg aagactttga gattgaaggc tacgatccac
     6961 atccgggcat taaagcgccg gtggctatct aattacgaaa catactgcca gagccgacgc
     7021 cagtgtgcgt cggttttttt accctccgtt aaattcttcg agacgccttc ccgaaatttt
     7081 gcaacgtcct gcaacggcgt aaatagtccg gaagatgcgc cgaagaaata gaaactggcc
     7141 ctcgtctttt tttctcctcg ctccatactg ccggcatgaa aacacaacgt ggttatacgc
     7201 tgattgaaac gctggtcgcg atgctgattt tggtcatgct aagcgcaagt ggactctatg
     7261 gctggcaata ctggcagcag tcgcaacggc tttggcaaac cgccagctag gcgcgggact
     7321 atttgctcta tttacgtgaa gatgccaact ggcataaccg cgaccacagt atcagcgtta
     7381 tcagggaggg gacgttatgg tgccttgtga gttccgctgc tggggccaat acctgtcatg
     7441 gcagttcacc attggtcttt gtgccacgct ggcccgaagt cgaaatgagc gacctgacac
     7501 cttcgcttgc tttctttggc ctgcgcaata ccgcatgggc cgggcatatt cgcttcaaaa
     7561 actcaacggg cgagtggtgg ctggtggttt cgccgtgggg aagactccgg ctttatcagc
     7621 aaggagaaac agaaggatgc ctgtaaaaga gcaaggtttt tctctgctgg aagtgttgat
     7681 tgctatggcg atcagtagcg tattgttgct gggggctgca cgctttctgc ctgcgttaca
     7741 gcgtgaaagt ttaacgagca ctcgtaagct ggcactggaa gatgaaatct ggctgcgggt
     7801 atttaccgtc gcgaagcatc tccagagggc gggttattgt catggcagct gtaccggcga
     7861 agggctggaa attgtcggac agggtgactg tatcattgtg cagtgggatg cgaacagtaa
     7921 cggtatctgg gatcgcgaac cggtaaaaga gtccgaccag attggatttc gtctgaagga
     7981 gcatgtgctg gaaacgctac gcggtgcgac atcctgtgaa ggtaagggct gggataaagt
     8041 cactaatccg gatgccatca ttatcgacac ttttcaggtc gtacgtcagg atgtcagcgg
     8101 cttctcgccg gtgttgacgg ttaatatgcg tgctgccagt aagtctgaac cgcaaaccgt
     8161 ggtggatgcc agctatagcg tgacaggatt caacctgtga accgcgaaaa aggtgtttcg
     8221 tcacaggctc tggtcctgat gctgctggtt ttgggtagct tgctattgca aggaatgagt
     8281 cagcaggatc gcagttttgc ctctcgcgtg agcatggaaa gtcagtcatt gcgccgccag
     8341 gccatcgttc agtcggcgct ggcgtgggga aaaatgcact cctggcagac gcagccagca
     8401 gttcagtgct cgcagtacgc tggaaccgat gcccaggttt gtttgcgttt actggcagat
     8461 aatgaagcct tattgattgc cggttatgaa ggcgtttcgt tgtggcgaac aggcgaagtc
     8521 attgatggaa aaattgtttt ttcgccacgc ggctggagcg atttttgtcc gctgaaagag
     8581 ggggcattat gtcagcttcc ctgaagaatc aacaaggctt tagcctgccg gaggtaatgg
     8641 ttgcgatggt gctgatggtg atgattgtca ctgcgttatc gggtatccag cgaacattaa
     8701 tgaacagtct cgccagcaga aaccagtacc aacagctctg gcggcatggt tggcagcaaa
     8761 cgcaactgcg cgcgatttcg ccacctgcca actggcaggt caaccgaatg cagacatcgc
     8821 aggcgggatg tgtcagcatc agcgttacgc tagtttcacc cgggggcaga gaaggcgaga
     8881 tgacccgcct gcattgcccg aatcgtcagt agtcaggagc cgctatgtta agggtctacc
     8941 attccaatcg tctggacgtg ctggaagcgt tgatggagtt tattgtcgaa cgcgaacggc
     9001 tggacgatcc tttcgaacca gagatgattc tggtgcaaag taccggtatg gcgcagtggc
     9061 tgcaaatgac cctgtcgcaa aagtttggta ttgcggcaaa cattgatttt ccgctgccag
     9121 cgagctttat ctgggatatg ttcgtccggg tgttaccgga aatccccaaa gagagcgcct
     9181 ttaacaaaca gagcatgagc tggaaactga tgactctgct gccgcaattg ctggagcgcg
     9241 aagactttac cctgttgcgg cattatctga ctgacgatag cgacaagcga aaactgttcc
     9301 agctttcctc aaaagcggcg gacctgtttg accagtatct ggtctatcgt ccggactggc
     9361 tggcacagtg ggaaacagga catttggttg aagggctggg agaagcacag gcctggcaag
     9421 ccccgttgtg gaaggcgctg gtggaatata cccatgaact cggacaaccg cgctggcacc
     9481 gcgcgaatct ctatcagcgc tttatcgaaa cgctggagtc cgcgacgacc tgcccgccgg
     9541 ggttaccttc gcgcgtcttt atatgcggta tttccgcgtt accgcctgtt tatctccagg
     9601 cgctccaggc gctgggtaaa catattgaaa tccatctcct gtttaccaac ccctgccgtt
     9661 attactgggg cgatattaaa gatcctgctt atctggcgaa actgctgact cgtcagcgtc
     9721 gacacagttt tgaagatcgc gaattgccgc tatttcgcga tagcgaaaat gccggacagc
     9781 tctttaacag cgatggtgaa caggatgtcg gcaacccgct gctggcctca tgggggaagc
     9841 ttgggcgcga ctacatttat ctcctttctg acctggagag cagccaggag ctggacgctt
     9901 ttgtcgatgt gacgccagat aacctgctgc ataacattca gtctgacatt ctggaactgg
     9961 aaaaccgcgc cgttgcgggt gtgaacatcg aagagttttc ccgtagcgat aacaaacgcc
    10021 cgcttgatcc actggatagc agtatcacat tccacgtttg ccatagcccg cagcgtgaag
    10081 ttgaagtttt acacgatcgc ttgctggcga tgctggagga agccccgaca cttactccgc
    10141 gcgacatcat cgtgatggtg gctgatatcg acagctacag tccgtttatt caggcagtgt
    10201 ttggtagcgc acctgcggat cgttacctgc cttacgccat ttccgaccgt cgtgcgcggc
    10261 agtcacatcc ggtactggaa gcgtttatca gcctgttatc actgcctgac agtcgctttg
    10321 tgtcagagga tgtgctggcg ctgctggatg tgccggtgct ggcggcgcgg tttgacatca
    10381 ccgaagaagg gctgcgttat ttacgccagt gggtcaacga atccggcatt cgttggggga
    10441 tagatgacga caacgttcgc gagctggaac tccccgccac cggacaacac acctggcgat
    10501 ttggcctgac gcgtatgttg ctgggctacg cgatggagag cgcgcagggc gagtggcaat
    10561 cggttctacc ttatgatgaa tcgagcggct taattgcaga actggtgggg catctggctt
    10621 cactgctaat gcagctaaac atctggcgtc gcgggctggc acaggagcgt ccgctagaag
    10681 agtggttgcc ggtttgtcgc gatatgctca acgccttctt cctgccggat gcggaaaccg
    10741 aagcggcgat gacgctgatc gaacaacaat ggcaggcgat tatcgccgaa ggtttaggtg
    10801 cgcagtatgg cgacgcggtg ccgctgtcac tattgcgtga tgaactggca cagcgtctgg
    10861 atcaagaacg tatcagccag cgttttctcg ccggaccggt taatatttgt actctgatgc
    10921 caatgcgttc aattccgttc aaagtggttt gcctgctggg aatgaacgac ggcgtttatc
    10981 cacgtcagct tgcgccattg ggctttgacc tgatgagcca gaaaccgaag cgtggcgacc
    11041 gtagccgtcg cgatgacgac cgctacctgt tcctggaagc gttaatttcc gcgcagcaaa
    11101 aactctatat cagctatatc gggcgttcca ttcaggataa cagtgaacgt ttcccgtcgg
    11161 tactggtgca ggaactgatc gactacatcg ggcaaagtca ttatctaccg ggcgatgaag
    11221 cgctcaactg tgatgaaagc gaggcaaggg taaaagcgca tcttacttgc ctccataccc
    11281 ggatgccgtt tgatccgcaa aactaccagc caggcgaacg acaaagctat gctcgtgaat
    11341 ggctacctgc ggccagccag gctggtaaag cacattctga atttgttcag ccgctgccgt
    11401 ttaccttacc ggaaaccgtg ccgctggaaa cgctacaacg attctgggca catccggtgc
    11461 gggcgttctt ccagatgcgt ttgcaggtga acttccgtac tgaagacagc gaaatcccag
    11521 acaccgagcc atttattctg gaaggactta gccgttatca aatcaatcag cagttattga
    11581 atgcactggt tgagcaggat gatgccgaac gcttgttccg ccgcttccgg gcggcagggg
    11641 atttaccgta tggcgctttt ggtgaaattt tctgggaaac acagtgccag gagatgcagc
    11701 agcttgccga cagagtcatt gcctgtcgcc agccggggca gagtatggaa attgatctcg
    11761 cctgcaacgg tgtgcagata actggctggt tgccgcaggt gcagccggat ggcctgttgc
    11821 gctggcgtcc ctctttatta agtgtggcgc agggaatgca actttggctg gaacaccttg
    11881 tctactgtgc cagcggtggt aatggcgaaa gtcgcctttt tctacgcaaa gacggtgagt
    11941 ggcgttttcc gccgcttgca gccgaacagg ctttgcatta cctctcacaa ctgattgagg
    12001 ggtatcgtga aggaatgtcc gcgccattgc tggtgttacc tgaaagtggc ggcgcgtggc
    12061 taaaaacctg ttatgacgcg caaaacgatg ccatgctgga tgacgattcc acgttgcaaa
    12121 aagcccgtac gaaattcctt caggcttacg aaggcaacat gatggtgcgt ggcgaaggtg
    12181 atgatatctg gtatcaacgg ctctggcggc aattaacacc agagacaatg gaggccatcg
    12241 ttgaacagtc gcaacgtttc ctgttaccgc tgtttcgctt taatcagtca tgagtgttgt
    12301 ataaaaattg cgcaatctat ccgcttactt tatgatgcgc accagtcacg gactgatggt
    12361 tatataaaca taggctgact cgtgcagcac aagattaaat tctggcagat gatttgcgtt
    12421 aacctgttga atctgtccag aaaataaagt tgattatgag gtccgtgaat gccccgcagc
    12481 acctggttca aagcattatt gttgtttgtt gccctctggg caccattaag tcaggcagaa
    12541 acgggatggc agccgattca ggaaaccatc cgtaaaagtg ataaagataa ccgccagtat
    12601 caggctatac gtctggataa cggtatggtg gtcttgctgg tttctgatcc gcaggcagtt
    12661 aaatcgctct cggcgctggt agtgcccgtt gggtcgctgg aagatcccga ggcgtaccag
    12721 gggctggcac attaccttga acatatgagt ctgatggggt cgaaaaagta cccgcaggct
    12781 gacagtctgg ccgaatatct caaaatgcac ggcggtagtc acaatgccag cactgcgccg
    12841 tatcgcacgg ctttctatct ggaagttgag aacgacgcct tgcccggtgc ggtagaccgc
    12901 atggccgatg ctattgcaga acccttgctc gacaagaaat acgccgaacg tgagcgtaat
    12961 gcagtgaacg ctgaattaac catggcgcgt acgcgtgacg ggatgcgcat ggcacaggtc
    13021 agcgcagaaa ccattaaccc ggcacacccc ggttcaaagt tttctggtgg taacctcgaa
    13081 actttaagcg acaaacctgg taatccggtg cagcaggcgc tgaaagattt ccacgagaag
    13141 tactattccg ccaatttgat gaaggcggtt atttacagta ataaaccgct gccggagttg
    13201 gcaaaaatgg cggcggacac ctttggtcgc gtgccgaaca aagagagcaa aaaaacggaa
    13261 atcaccgtgc cggtagtcac cgacgcgcaa aagggcatta tcattcatta cgtccctgcg
    13321 ctgccgcgta aagtgttgcg cgttgagttt cgcatcgata acaactcagc gaagttccgt
    13381 agtaaaaccg atgaattgat tacctatctg attggcaatc gcagcccagg tacactttct
    13441 gactggctgc aaaagcaggg attagttgag ggcattagcg ccaactccga tcctatcgtc
    13501 aacggcaaca gcggcgtatt agcgatctct gcgtctttaa ctgataaagg cctggctaat
    13561 cgcgatcagg ttgtggcggc aatttttagc tatctcaatc tgttacgtga aaaaggcatt
    13621 gataaacaat acttcgatga actggcgaat gtgctggata tcgacttccg ttatccgtcg
    13681 atcacccgtg atatggatta cgtcgaatgg ctggcagata ccatgattcg cgttcctgtt
    13741 gagcatacgc tggatgcagt caatattgcc gatcggtacg atgctaaagc agtaaaggaa
    13801 cgtctggcga tgatgacgcc gcagaatgcg cgtatctggt atatcagccc gaaagagccg
    13861 cacaacaaaa cggcttactt tgtcgatgcg ccgtatcagg tcgataaaat cagcgcacaa
    13921 actttcgccg actggcagaa aaaagccgcc gacattgcgc tctctttgcc agagcttaac
    13981 ccttatattc ctgatgattt ctcgttgatt aagtcagaga agaaatacga ccatccagag
    14041 ctgattgttg atgagtcgaa tctgcgcgtg gtgtatgcgc caagccgtta ttttgccagc
    14101 gagcccaaag ctgatgtcag cctgattttg cgtaatccga aagccatgga cagcgcccgc
    14161 aatcaggtga tgtttgcgct caatgattat ctcgcagggc tggcgcttga tcagttaagc
    14221 aaccaggcgt cggttggtgg cataagtttt tccaccaacg ctaacaacgg ccttatggtt
    14281 aatgctaatg gttacaccca gcgtctgccg cagctgttcc aggcattgct cgaggggtac
    14341 tttagctata ccgctacgga agagcagctt gagcaggcga agtcctggta taaccagatg
    14401 atggattccg cagaaaaggg taaagcgttt gagcaggcga ttatgcccgc gcagatgctc
    14461 tcgcaagtgc cgtacttctc gcgagatgaa cggcgtaaaa ttttgccctc cattacgttg
    14521 aaagaggtgc tggcctatcg cgacgcctta aaatcagggg ctcgaccaga gtttatggtt
    14581 atcggcaaca tgaccgaggc ccaggcaaca acgctggcac gcgatgtgca aaaacagttg
    14641 ggcgctgatg gttcagagtg gtgtcgaaac aaagatgtag tggtcgataa aaaacaatcc
    14701 gtcatctttg aaaaagccgg taacagcacc gactccgcac tggcagcggt atttgtaccg
    14761 actggctacg atgaatacac cagctcagcc tatagctctc tgttggggca gatcgtacag
    14821 ccgtggttct acaatcagtt gcgtaccgaa gaacaattgg gctatgccgt gtttgcgttt
    14881 ccaatgagcg tggggcgtca gtggggcatg ggcttccttt tgcaaagcaa tgataaacag
    14941 ccttcattct tgtgggagcg ttacaaggcg tttttcccaa ccgcagaggc aaaattgcga
    15001 gcgatgaagc cagatgagtt tgcgcaaatc cagcaggcgg taattaccca gatgctgcag
    15061 gcaccgcaaa cgctcggcga agaagcatcg aagttaagta aagatttcga tcgcggcaat
    15121 atgcgcttcg attcgcgtga taaaatcgtg gcccagataa aactgctgac gccgcaaaaa
    15181 cttgctgatt tcttccatca ggcggtggtc gagccgcaag gcatggctat tctgtcgcag
    15241 atttccggca gccagaacgg gaaagccgaa tatgtgcatc ctgaaggctg gaaagtgtgg
    15301 gagaacgtca gcgcgttgca gcaaacaatg cccctgatga gtgaaaagaa tgagtgatgt
    15361 cgccgagaca ctagatcctt tgcgcttgcc cttacagggc gagcgcctga ttgaagcctc
    15421 tgccggcaca ggcaaaacct ttacgattgc ggcgctctat ttgcgcctgt tacttggatt
    15481 aggcggttcc gccgcctttc cccgcccgct gatcgttgaa gaactgctgg tggtcacctt
    15541 taccgaggct gccacggcag aattgcgcgg tcgtatccgt agcaatatcc acgagttgcg
    15601 catcgcctgt ctgcgtgaaa ccaccgacaa tccactgtac gaacgcctgc tggaagagat
    15661 cgacgataaa gcgcaagccg cgcagtggtt gttgttagcc gaacggcaga tggatgaagc
    15721 ggcagtcttt actattcacg gcttttgcca gcgcatgctc aacctgaatg cctttgaatc
    15781 cggcatgctg tttgagcagc agctgattga agatgagtct ctgctacgct accaggcctg
    15841 cgccgatttc tggcgtcgcc actgctaccc gctgccgcgt gaaatagccc aggtcgtctt
    15901 tgaaacctgg aaagggccgc aggcgttgct gcgcgatatt aatcgttatc tgcaaggcga
    15961 agcgccggtt atcaaagcac cgccgcccga tgatgaaacg ctggcttccc gccacgcgca
    16021 aattgtggcg cgtattgata cggtaaaaca gcagtggcgc gacgcagtgg gtgaactgga
    16081 tgcgctgatc gaatcttctg gtattgatcg acgcaagttt aaccgtagca atcaggctaa
    16141 atggatcgaa aagatcagcg cttgggcaga agaagagaca aacagttatc agttgccgga
    16201 gtcgctggaa aaattctccc agcgtttctt agaagatcgc acgaaggccg ggggggaaac
    16261 cccgcgacat ccactgtttg aggtgatcga tcaactgctt gcagaaccat tgtcgatccg
    16321 cgatctggtg atcacccgag cattggctga gatccgcgaa acagtagcgc gtgaaaaacg
    16381 ccgccgtggc gaattgggtt ttgatgacat gttaagtcgg ctcgattccg cgctgcgtag
    16441 cgaaagcggc gaggtgttgg cagcggcgat ccgtacgcga ttcccggtgg caatgatcga
    16501 tgaatttcag gataccgacc cccagcagta ccgaattttt cgccgtatct ggcaccatca
    16561 gccggaaacc gcattgttgc tgattggcga cccgaagcag gccatatatg cattccgggg
    16621 tgcggatatc ttcacttata tgaaggcgcg tagtgaagtt cacgcccact acactttaga
    16681 caccaactgg cgctccgcac ctggaatggt gaacagtgtg aacaagcttt tcagccagac
    16741 tgatgacgcg ttcatgtttc gcgaaatacc gtttattcca gtgaaatcag ccgggaaaaa
    16801 tcaggcgtta cgttttgtat ttaaaggtga aacgcagcct gcgatgaaaa tgtggctgat
    16861 ggaaggcgaa agctgcggcg ttggcgacta tcaaagtacc atggcgcagg tatgtgctgc
    16921 gcaaatccgc gactggctac aagccggaca gcggggcgaa gcgttgctga tgaacgacga
    16981 cgacgcgcgt ccggtgcgtg cttcggacat cagcgtgctg gtgcgcagcc gccaggaggc
    17041 cgcccaggtg cgcgatgcct taacgttgct ggaaatccct tccgtttacc tttcgaaccg
    17101 cgacagtgtt tttgaaactc tggaagcgca ggaaatgctt tggttgttgc aggcggtgat
    17161 gacgcccgaa cgtgagaaca ccctgcgcag tgcgctggca acgtcaatga tggggctgaa
    17221 tgcgctggat attgaaacgc tgaataatga cgaacatgcg tgggatgcgg tagtcgaaga
    17281 gttcgatggt tatcggcaaa tctggcgcaa acgtggcgtt atgccgatgc tgcgggcgct
    17341 gatgtcggcg cgtaacattg cagaaaactt gctggcaacg gcaggcggtg agcggcgtct
    17401 taccgatatc ttgcatatca gcgaactgct tcaagaagcc ggaacgcagc tggaaagtga
    17461 acatgcgctg gtacgctggt tatcgcaaca tatcctcgag ccagacagta atgcctccag
    17521 ccaacaaatg cgcctcgaaa gtgataaaca tctggtgcag attgtcacga tccataaatc
    17581 gaaagggctg gaatatccgc tggtctggtt gccatttatt accaatttcc gcgtccagga
    17641 tcaggcgttt tatcacgatc gccactcgtt tgaggcagtt ttggatctta atgctgcgcc
    17701 agaaagcgtc gacctcgcgg aggtcgaacg tctggcggaa gatctgcgtt tgctttacgt
    17761 ggcgctgacg cgttcggttt ggcattgcag tctcggcgtt gcaccgctgg tgcgccgtcg
    17821 tggcgataaa aaaggtgaca ccgacgtcca ccaaagtgcg ctcgggcgtt tgctgcaaaa
    17881 aggggaaccg caagatgcgg cagggcttcg cacctgtatt gaagcgttat gcgatgatga
    17941 tattgcctgg caaacggcac aaactggtga taaccagccc tggcaggtta atgatgcttt
    18001 aactgcagaa ctgaatgcga ggacactaca acgattgccc ggcgataact ggcgtgtcac
    18061 cagctactcc ggcttgcagc agcgtggtca cggtatcgct caggatctga tgccacggct
    18121 ggatgttgat gccgcaggcg tggtcagcgt cgttgaagaa ccgacgttaa caccgcatca
    18181 gttcccgcgc ggtgcgtcac cggggacatt cttgcacagt ttgtttgaag acctcgattt
    18241 tacccagccg gttgacccga actgggtaca ggaaaaactg gaactcggcg gctttgaatc
    18301 gcagtgggaa ccggtgttga ccgagtggat cacggctgtc ctccaggcac ctctcaatga
    18361 aacgggtgtt agcctgagtc agctttccga ccgcgataaa caggtggaga tggagtttta
    18421 cctgccgatt agcgaaccgc tcatcgccag ccagcttgat gcactaattc gccagtttga
    18481 cccgctatcc gctggctgcc cgccgctgga gttcatgcag gtacgcggca tgttaaaagg
    18541 ctttatcgac ctggtattcc gccacgaagg gcgttattac ctgctcgact ataaatccaa
    18601 ctggctgggt gaagacagtt cggcttacac ccaacaggct atggcagcag cgatgcaggc
    18661 acaccgctat gatctgcaat atcagcttta taccctggca ctgcatcgtt atctgcgcca
    18721 tcgcattgct gattacgact atgatcttca ctttggcggc gttatctatc tgttcctgcg
    18781 tggcgttgat aaagaacatc cgcaacaagg gatttacaca acccgaccca acgccgggtt
    18841 gattgccctg atggatgaga tgtttgccgg tatgaccctg gaggaggcgt aatgaaattg
    18901 caaaagcaat tactggaagc tgtggagcac aaacagctac gcccgctgga tgtgcaattt
    18961 gccctgaccg tggcgggaga tgaacatcct gctgtcaccc tcgcggcggc actgttaagt
    19021 catgatgccg gagagggaca cgtctgtttg ccgctttcac gactggaaaa taacgaggcg
    19081 tcgcatccgc tgttggcgac ctgtgtcagt gaaatcggtg agctacaaaa ttgggaagaa
    19141 tgcttgctgg cttctcaagc ggtcagcagg ggagatgaac ccacgccgat gatcctctgt
    19201 ggcgatcgtc tttatttgaa tcgcatgtgg tgtaacgagc ggacggtagc gcgcttcttc
    19261 aatgaggtga atcataccat tgaggttgat gaagctctac tggcgcaaac cctggacaaa
    19321 ctttttccag taagcgatga aattaactgg caaaaagttg cagcggcagt ggcgctgacg
    19381 cggcggatct cggtgatttc cggcggccca ggcaccggta aaacgaccac cgtggcgaag
    19441 ttgctggcag cgttaattca aatggccgac ggcgaacgct gccgtatccg tctggctgca
    19501 ccaacgggta aagctgccgc gcgcttaacc gaatctctcg gcaaggcttt gcgacagtta
    19561 ccgctgaccg atgaacaaaa gaaacgcatt ccggaagatg ccagcacttt gcaccgattg
    19621 ctgggcgcgc agccgggtag ccagcgttta cgtcatcatg ccggtaaccc gctgcatctt
    19681 gatgtgctgg tggtagatga agcgtcaatg atcgatctgc ctatgatgtc gagactgatc
    19741 gacgccttgc cagatcatgc gcgagtgatc tttctcggcg atcgtgatca actggcctcg
    19801 gttgaggctg gggctgtgct gggcgatatc tgcgcttatg ccaacgcggg ctttaccgcc
    19861 gagcgtgcca ggcagctaag ccgcctgacg gggactcacg ttccggcagg aaccggcaca
    19921 gaagcggcat ctttgcgcga cagtctctgc ctgctgcaaa aaagctatcg tttcggcagc
    19981 gattctggca ttggtcagtt agctgcggcg atcaaccgtg gtgataaaac ggcagtgaaa
    20041 accgtttttc agcaggattt tactgatatc gaaaaacgac ttttacagag cggcgaagat
    20101 tatattgcga tgcttgagga agctcttgcg ggttacggac gttatctgga tctgctgcaa
    20161 gcgcgtgccg agccggattt aatcattcag gcgttcaatg agtaccagct tttgtgcgcc
    20221 ctgcgggaag ggccgtttgg cgtggctgga ctgaatgagc gaattgagca gtttatgcaa
    20281 cagaagcgca aaattcatcg tcatccgcac tctcgttggt acgaaggccg accggtgatg
    20341 attgcccgta atgacagcgc gcttgggttg tttaatggcg atatcggtat tgcgctggat
    20401 cgcgggcagg ggacgcgcgt ctggtttgcg atgccggacg gcaatattaa gtctgtgcaa
    20461 ccgagtcgct tgccagagca cgaaactacg tgggcgatga cggtacataa atcgcaggga
    20521 tcggagttcg accatgcggc gttgatttta ccgagccaac gcacgccggt agtaacgcga
    20581 gagctggtat acaccgcggt gacccgcgcg cgtcgtcgtc tgtcgctgta tgccgatgag
    20641 cgtatattaa gtgcggcaat cgccactcgt actgagcggc gcagtggtct ggcggcgttg
    20701 tttagttcgc gggaataaac gtaattgccg gatgcgacgc acgagtgtta cgcctgccgc
    20761 atccggtgat tttcatcgct taccctaaat ccgccatcaa cactttggat ttacgctggt
    20821 agttgtacaa ctgctttttg ctctcgggca gtaaatcaat atccaccggg gtaaatccac
    20881 gttcctggaa ccagtgaata ctgcgcgtgg tcagcacaaa caatttgctt aagccgctct
    20941 gcttcgcctg agcggcaatg cgttccagca gaacctcgcc ccgtgatgaa ctgcggtaat
    21001 ccgggtgaac tgccacacag gccatttccc caatcttctc ttccgggaac ggatagagcg
    21061 cggcgcaggc aatagtcgtg ttatcgcgct gaataatggt gaatttgtcg atttccatct
    21121 ccagctgctc gcgagaacgg cgcaccagaa taccttgctg ctccagcggg cgaatcaact
    21181 ccagaatacc gccaatatcg ttgattgttg cgcgacgaat ctgctcggcg ctttccatca
    21241 caatctgcgt accgataccg tcgcgtgaga acaactcttg caacagcgcg ccatcttcct
    21301 gataactgat taaatgacag cggcgcacgc cgctgcggca ggctttcact gcgccacgca
    21361 aaaagcgcac cgtaccggag ttgtaatcgc ctttctcttc ctgggcttct acccgtgctt
    21421 gcgcttcgtt agggaaaagt tcggagacaa tatcaccgtc gtcattagta acgccctggg
    21481 aagagcaaaa accaatcatc ttttcagctt tcagtttgat ggctagttga gtggcaatct
    21541 cttcagaggt cagattaaag ctctcgccag tgactgaaac agcgaccggc cccattagca
    21601 ctattgcacc gctttccagt tgacgatgga tcgcgtcttc atcaatccgc cggatacgcc
    21661 cgctatggca gtaatccacg ccgtcatcga cgcccagcgg ctgggcaata ataaaattgc
    21721 cactaacgac gttgatatgc gcgccctgca gcggcgtgtt attgagactc attgacaggc
    21781 gagcagtaat atccagttgc aatgttcccg cagcctgctt caccagttcc agtgttttgg
    21841 cgtcggtcac acgtatattc ttgtgataca gcggttcgtg gtgatgcgca gccagatttg
    21901 cgtcgatctg cggacgtgcg ccatagacca ccaccagacg gatgccgagg ctgtgcaaca
    21961 acccgatatc attaacgata ctggagaaat tctcatgctc aatggcttca ccgccgagca
    22021 tgatgacaaa cgtttttccc cggtgggtat tgatataggg aaccgaatgg cggaatccct
    22081 cgaccagctc ggttttacgt tcctttacca cggcacaccc ctttgcatga ttatttgaaa
    22141 ttattgtatt tttattctgt tttttcgcag ggtgcaagtg taaattttat gcgagagcga
    22201 cttttttatc agtaatgccg tgaatataaa aagaatgttt accgtttatt agatgacaga
    22261 ttatgcgtct ttcgctaaag tttccggtca aattagtcgt ttacttgtta cacagcttag
    22321 atttcttttg ctcgggagag gcatgtcagg atccaacact gcaatcagcc gtcgtcgttt
    22381 actgcaaggc gcgggcgcta tgtggctatt gagcgtaagt caggtcagcc tggctgcggt
    22441 tagccaggtc gtggcggtgc gcgtctggcc tgcgtccagc tacacccgcg tgacggtaga
    22501 atcaaatcgt cagctgaaat ataagcagtt cgcgttgagt aaccctgaac gcgtggtggt
    22561 ggatatcgaa gatgtaaacc tgaactcggt actcaagggg atggcggcac aaattcgtgc
    22621 agacgacccg ttcatcaagt cggcgcgcgt cgggcaattt gacccgcaaa ccgtacgcat
    22681 ggtttttgaa ttaaagcaaa acgtaaaacc gcagctgttt gcccttgcgc cggtcgccgg
    22741 gtttaaagag cgtctggtga tggacctcta tccggccaat gcacaggata tgcaggaccc
    22801 gctgctggcg ctgctggagg attacaacaa aggcgacctc gaaaagcagg tgccgccagc
    22861 gcaaagtggt ccacaaccgg gtaaagcagg gcgcgatcgt ccgattgtca ttatgcttga
    22921 ccctggccac ggtggcgaag attccggtgc ggtggggaaa tacaaaacgc gcgaaaaaga
    22981 tgtggtattg caaatagctc gccgtttgcg ctctctgatc gagaaagagg gcaatatgaa
    23041 ggtgtacatg acgcgcaatg aagacatctt cattccgttg caagtgcgcg tagcaaaagc
    23101 ccagaaacag cgtgctgacc tgtttgtctc tatccatgcc gacgccttta ccagtcgtca
    23161 gccgagcggt tcctcggtgt ttgcgctctc aaccaaaggc gcaaccagta ctgcggcaaa
    23221 atatctggca caaacccaga acgcctcgga cttgattggt ggcgtgagca aaagcggtga
    23281 ccgctatgtc gaccacacca tgttcgatat ggtgcagtcg ctgactattg ctgacagcct
    23341 taagtttggt aaagcggtac tgaataagct cggaaaaatc aacaagctgc ataaaaatca
    23401 agttgaacag gccgggtttg ccgtactaaa ggcaccagat attccctcca ttctggtcga
    23461 aacggcgttt atcagtaacg ttgaggaaga gcgtaaactg aaaacggcga ctttccagca
    23521 ggaagttgcg gagtctattc ttgcggggat taaagcgtat tttgccgatg gggcaacgct
    23581 ggcgagaagg ggatgataaa aaggcgctga atggcgcttt ttttatggga gatcagaaac
    23641 aaaaaaacac ccgttagggt gttcaataat tggttgcggg ggccggattt gaaccgacga
    23701 ccttcgggtt atgagcccga cgagctacca ggctgctcca ccccgcgtca ccgtactgct
    23761 tttacttcat caaatttgat tggttgcggg ggccggattt gaaccgacga ccttcgggtt
    23821 atgagcccga cgagctacca ggctgctcca ccccgcgtcc gtggatgcgc actatactcc
    23881 gctcgcgttt tcatgcaacc ttttttttac cccgatcacg gatttagcat gattttgaac
    23941 aaaaacaaac cactaaagct tattttttgt gcgaaatttg cgtcagggag aggctgctca
    24001 tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt attttttaac ctgaagaaga
    24061 gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg cacggttgtg gcaatgcttg
    24121 ccgcctgctc ttccaaacca accgatcgcg gacagcaata taaagacggg aaatttaccc
    24181 agcctttctc tctggtgaac cagccagatg ccgttggcgc gccgattaac gccggtgatt
    24241 ttgccgagca aattaaccat atccgtaatt cgtcaccgcg tctgtatggc aaccagagta
    24301 atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg tgatacccgc aatatgcgcc
    24361 agttcggcat tgatgcctgg cagatggaag gtgccgacaa ctatggtaac gtgcagttta
    24421 caggctatta cacgccggta attcaggcgc gccatacccg ccagggcgag ttccagtatc
    24481 ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc tcgtgcggag atctacgccg
    24541 gggcattggg cgataaatac atcctcgctt acagcaactc tctgatggat aacttcatta
    24601 tggatgtgca gggtagtggg tatatcgact ttggtgatgg cagtccgctt aactttttca
    24661 gctatgcagg gaaaaacggt catgcctatc gcagcattgg taaggtgctg atcgaccgtg
    24721 gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg tcattggggc aaaacacaca
    24781 gtgaagccga ggttcgcgag ctgctggaac agaacccgtc tttcgtcttc tttaaaccgc
    24841 aatcttttgc accggtgaaa ggggcaagtg cggtgccgct ggttggccgt gcttctgttg
    24901 cctccgatcg ttcaattatt ccgccaggta ctaccttgct ggcagaagtg ccgctgctgg
    24961 ataataacgg caaatttaac ggtcagtacg aattgcgtct gatggtggcg ttggatgtcg
    25021 ggggcgcaat caaaggccaa cacttcgata tctatcaagg gatcgggccg gaagccggac
    25081 accgcgcagg ttggtacaac cactatggac gggtttgggt gctgaaaacg gctccgggcg
    25141 caggtaacgt ctttagcggc tgatgtgata ttctgtatgc cgtttttacg gatatgacag
    25201 ggtgaggggt aacctcacat cctgattatt gactaagtgc gcgttgttca taccggatgc
    25261 ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc aaaatattgc aggagttatg
    25321 taggcctgat aagcgtagcg catcaggcat ttatgcgttt agggtgaggg ataacctcgc
    25381 cctttttaat tctgaggtgc tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt
    25441 ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac tgtttgctga cgcgcatatt
    25501 tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg aagcgctggc gcgcacgggg
    25561 attggcgcaa tcacgcttat cgatatggat gatgtgtgcg tcaccaatac caatcggcaa
    25621 attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg aagtcatggc ggagcgcatt
    25681 cgccagatta acccggagtg ccgtgtaacg gtggtggatg atttcgtgac gccggataac
    25741 gtagcgcaat atatgagtgc cggttattcg tacgtgattg atgccattga tagtgtacgg
    25801 cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt
    25861 ggcgcgggtg ggcagattga cccgacgcag attcaggtta ccgatctggc gaaaacgatt
    25921 caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa
    25981 aacagtaaag gtaagctcgg cgtggattgc gtgttttcta ctgaagcgct ggtgtacccg
    26041 cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag aagggccgaa gcggatggat
    26101 tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca cctttgggtt tgttgcggtt
    26161 tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc agggttaaac aatcaggtcg
    26221 gatgagacgc gaacgtctta tccgacccgg ttctgtgctt aaacctgctt cgtcgcagcg
    26281 ataatcgcct cgcttaacgc atttaacccc tggctgcgtg aggcgctaag ctgcgcacgt
    26341 aatcccagct catcaaacaa tgccagcggc gactgtgcct gtaattcggc ggcggttttc
    26401 ccctcaacgg cagtcaacaa caccgccaac aggccgcgca caatgcgccc ttcgctgtcg
    26461 ccaaagaagt gcattttgcc gttttcagcc acggtatatc ccagccagac gcggttttcg
    26521 catccggcaa tctctttagc ctgcgctttt aactcgtctg gcaaagccgg aagctgtttc
    26581 cccagcatga tcaactggcg atatttatct tcccattgcg acaacggtgc gaaggtattg
    26641 cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc
    26701 attaatccac caataattcc agcgcgcggt caacggcatt caccagcgca tccacatcac
    26761 tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc ggttacgcct aattctgcca
    26821 gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat accgtactcc gccagtagcg
    26881 tcaccatatc gctgtgatga acgccagcaa aatcaaaggc cagcaggctg gaatcctggc
    26941 agcggaatga acgaaagcct ggacgtttcg ccagcgcttc ttctgcaagc gttgctaagc
    27001 tacggctcca gctttcggcc tggttgatat cgtaatctgc cagccattcc agcgccgcgc
    27061 ttaatcctat gacgccagcg acatttggcg ttcctgcttc cagtttccac ggcgcagatt
    27121 gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt gccgccaccc agccagggcg
    27181 acatcgcttc cagcagttct gatttgccat acagcacgcc gatacccgtc gggccataca
    27241 gtttgtgacc agagaaagca tagaaatcga tatccagttg ctgaacatcc gcggggaaat
    27301 gcaccgcccc ctgagcacca tcaaccatca ccaccatccc ggctgaatga gcaaaggtaa
    27361 tcgctcgcgc cagatccggg caaccgccag tcacgttcga catctgaccc aacgccagaa
    27421 tccgactacg gggagtaatc agttctggca acaaatcgac atccggcagt cgctgcgcat
    27481 taagcggcaa tttcaccact ttggctctag tttgttgggc gaccatcagc caggggacga
    27541 ggttagcgtg gtgttctgcc acgctgacaa taatctcatc gcccggttgc agacgcggac
    27601 gcgcatagca ttgtgccacc atgttgatgg attcagtggt gccgcgcgtc cagacgatag
    27661 ttttatcatc cggtgcattc agtaattgcg ccactttctc tcgtgcagct tcataacgcg
    27721 cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac gtttccggcg ctcaggctgt
    27781 aaaactgtcg agtggcttca accacggctt caggtttaag cgcggtcgcg gcgctgtcga
    27841 gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg aaactgcgcg ggattaaaaa
    27901 cgttcatggt actcctcggc ttgatgcacc gatcgtgacg caaaattcac tttgatacaa
    27961 ggagagtgct aaccatcaga attatctaga tatcctggcg aagccgcgta agtaacatat
    28021 gctgaatagt gttaggcgtt aattttagcc tgttaatcaa tactattaag atttattaat
    28081 cgctttaagg agaagatgat gaaaaagact gccgcaatta tttctgcctg tatgctgact
    28141 tttgccctga gcgcctgttc cggttcgaac tatgtgatgc acaccaatga cggacgtacc
    28201 atcgtctctg acggcaaacc acagactgat aacgataccg gtatgatttc gtataaagac
    28261 gctaatggca acaaacagca gatcaaccgt actgacgtga aagagatggt cgaactggaa
    28321 cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc
    28381 actatggaca gacagggtaa atgtacagga agtgaaaaaa ggtagctttg ctaccatggt
    28441 ctgaatcgca gaccaattgc aaacacaaca acacaacatc acaaccgtaa gccaaaagtt
    28501 caccagaata cgcattccga taaaactttt cgttccggct caggaagtgc cgccactata
    28561 ggtatttgct ggtagaagct caacggacaa tttataatgg ctcagattaa aaaaactaat
    28621 aggttacaca gtgtgatcta attgttaaat tcatttaaca tcaaagttta aaagccatgt
    28681 ctaaacgatt accaccgcta aatgccttac gagtttttga tgccgcagca cgccatttaa
    28741 gttttactcg cgcagcagaa gagctttttg tgacccaggc cgcagtaagt catcaaatca
    28801 agtctcttga ggattttttg gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc
    28861 tgaccgagga agggcaaagc tatttcctcg atatcaaaga gatattttcg caattaaccg
    28921 aagcgacgcg taaactccag gcccgtagcg ccaagggggc gttgacggtc agtttactcc
    28981 ccagtttcgc cattcattgg ttggttccgc gactttccag ctttaattca gcttatccgg
    29041 gaattgacgt tcgaatccag gcggttgatc gtcaggaaga taagctggcg gatgatgttg
    29101 atgtggcgat attttatggt cggggcaact ggccggggct acgggtggaa aaactgtacg
    29161 ccgaatattt attgccggtg tgttcgccgc tactgctgac aggcgaaaaa cccttgaaga
    29221 ccccggaaga tctggctaaa catacgttat tacatgatgc gtcacgccgt gactggcaga
    29281 catatacccg acagttgggg ttaaatcata tcaacgttca gcaagggcca atttttagtc
    29341 atagcgccat ggtgctgcaa gcggctattc acgggcaggg agtggcgctg gcaaataacg
    29401 tgatggcgca atctgaaatc gaggccggac gtcttgtttg cccgtttaat gatgttctgg
    29461 tcagtaaaaa tgctttttat ctggtttgtc atgacagcca ggcagaactg ggtaaaatag
    29521 ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc
    29581 gttatgaaca ataatttacg tagggtacga ccatgaccag ccgttttatg ctgattttcg
    29641 ccgccattag cggcttcatt tttgtggcac tgggcgcttt tggtgcgcat gtgttaagta
    29701 aaaccatggg ggccgttgag atgggctgga tccaaaccgg tctcgaatac caggcgtttc
    29761 atacgctggc gatcttaggt ctggcggtgg caatgcagcg tcgcatcagt atctggtttt
    29821 actggagtag cgttttcctc gcgttaggca cggtgctgtt cagcggcagc ctttattgcc
    29881 tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc ggttggcggc gtgagcttcc
    29941 ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc
    30001 atgaataagg ttgtattgct gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt
    30061 accgataaag ccggccagcg ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt
    30121 tatgtcattt atgaatgtta tcaacctgat gatggcgata agttaatccg tgagctgccg
    30181 ttcagttcat taatttttgc ccgccagtgg tttgtggtgg gggaactcct gcagcatttg
    30241 ccgccagaag atcgtattac ccccattgtc ggcatgttac agggtgtagt agagaagggc
    30301 ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca aagagttgct gaaattctgc
    30361 cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg ctggggtgct ggcgaactat
    30421 gaaacgccga agcgtccggt tgtgcatgta ttctttattg caccaggatg ctgctatacc
    30481 ggttactcat acagcaacaa taattcgccg ttctatatgg gcattccgcg cctgaaattt
    30541 ccggcagagg cgccgagtcg ttccacgctc aaactggaag aggcatttca tgtgtttatt
    30601 cctgcggatg agtgggatga acgcctggcg aacgggatgt gggcggtgga tttaggcgct
    30661 tgccctggcg gctggaccta ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac
    30721 aacggcccga tggcgcaaag tctgatggat accgggcagg tgacgtggct gcgggaagac
    30781 ggtttcaaat tccgtccgac acgcagcaat atctcctgga tggtatgcga tatggttgaa
    30841 aaaccggcta aagttgcggc attgatggcg cagtggctgg ttaatggctg gtgccgtgaa
    30901 accattttca atctcaaact gccgatgaaa aaacgctacg aagaagtatc acacaatctg
    30961 gcgtatattc aggcacagct tgatgaacat ggcataaatg ctcagattca ggcgcggcag
    31021 ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc gcatctgggc tgcggtgggt
    31081 ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc gtctccggcc tgctaccctt
    31141 tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc atcagaaata atgtgcgtta
    31201 cctcatccag atacccgatg cgggcttccc ctgaacgatt aaatttactt ttatcaatca
    31261 ataacaacga ttgcgcggca cgttttaata gcatcgattt gtaatcagcg ttgatcgcat
    31321 tggagtccca cagtgcgccg ctgctatcga tcccttcaca agaaaaaata aacagatcga
    31381 tttccagcga tttaagttgg gaaatcagcg agggattgac gtaacagcca tatttgcgct
    31441 caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg tttaccgagt tcatggcaaa
    31501 tcggatggct attggtgaag acctgaatgt tgatgtcagg caactggcgt gccagatacc
    31561 agcaagttga actggcgtct aaggctatca ccatcctttc ttcaatccac gcgagcgcct
    31621 cgcgcgcgat atctgcttta tgcgcataat ggcttttcag cctgatgtga aagagagcgc
    31681 tactgtcttg attttgacgg tggatatatt tagcgcgtcc atgattgcgc agaattttac
    31741 cctgcgtctg taattcattg agatcgcgac gaatggtttc tttactgacc tttagctgtt
    31801 cagagagagc ttccgtggtc aggctggtat ggttcagcag caggtcgact atcgcttgct
    31861 ggcgtgccgc tttcatattt ttcaccccat cattcgataa ccgcccgcgt catgcgggcg
    31921 taccggtatg agattacggt gttacccctt tttttaaaag aatattcccg tacttcgctc
    31981 gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg ttcataaaac gcaaaacgat
    32041 tgatgcggat gatgtcagga cacggggctt gtagtgaaag cgcattacgg taacgtcgtt
    32101 ctacttcagg atcgagagtg tcaccctcta ccgccgccat catcaccagc ggcggggcat
    32161 aactgtccag ctcaaataac gggataatcg cctggagcaa gtcgctcacc aacaggccat
    32221 cagcgcggat cacctgcggt cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa
    32281 tttcatctcc atgtcccatc tctgccagca ctttcaatag ttcgggagaa attaacggcg
    32341 aaattgtttt cagcatttca cacttcctct ataaattcag gttcagtttg cgggtagaaa
    32401 taacggtact gataatgaat ctgtgcacgg gcttcttccg ggctgttaaa ttcccctacg
    32461 ccataccaac cgaacagcgc agctcctgcg acggtcgttt cggcatcgtc gagaactttt
    32521 accggaatat caagcatatt ggctttaatc tgattccaca atgtgttgcg acttcctcca
    32581 ccgactaaca ataattcata ggctttaaag tgcccgattt tttccagcat ctgtagattg
    32641 cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat agaaatgccc ccgcgtggta
    32701 ttaagcgtca ctccttgcca gccagcgttc tgacacgaca ataaatcaca ctgcattttc
    32761 acaccatccg cgccaggtgc gatcagacga gcttcttcaa tcaacatttg ccagggtgtt
    32821 tcagccgtcc agaacagttt tctcacccat tccagcacgc cggatgccag ccattgcata
    32881 cctgggttat acaaccatgc ctggctatct agttcgcagg tggaaccggc gtactgactt
    32941 aacagcgaag tatcaaccta ggcgctgcga accattaaaa tttcccatgt accggaggag
    33001 agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct
    33061 gcggaaatca ccggtatgcc aacgggtaag ccgagcattg ctgcggcgct gttctgtagc
    33121 gtaccaatct gttcacccgc ttccaccaga cgagggaaga gtcggcgtgg aataccggtg
    33181 gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat ccagcatctg gctggttccg
    33241 gctatcgtga tatcagtagt gaattcgccg gttaaacggt ggttaatcag cgacgaaatg
    33301 aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat tttctttcaa ccacaccaac
    33361 ttatataacg tattgaaact aaaggctccg acgccagaaa tagcctgcaa ccgctgtgcg
    33421 gagattaacc gttcaatatt gtccataacc gctgctgttc gcggacattt ccagctaatg
    33481 atcggataga gcagattgcc ttgcttatct accagagcgc catccacacc aaaggtggtg
    33541 acggcgatac cgcggatgtg gcattcagtc agttcactat tgatttgccg acagcaatca
    33601 gcaaagcgtt gcaaaatggc agtctaaaga ccactggtgc caggtgttgt tttccatcgc
    33661 gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc
    33721 gatggccctg acattggtcg cgccacagtc gagtaccagg ataacttctt gtttcataat
    33781 cgctcctgca atatagccgg ataacattgt ttatccggct aaccactctt gcttgttcag
    33841 gcccggtagc cggagcgacc gggcatcaca tcagggtgta atgtattaac gcttgtacaa
    33901 cggaccgtag ttctggcaag cgcggtaatc ctggccttca atatccatgc cgtgcgcagc
    33961 ccaggcagaa ggacgataca ctttggtctc ttcaacgttg tgcatacata ccgggatacg
    34021 cagcatggag gcaagggtga taaagtctgc gccaacgtgg ccgatggtca gaactccgtg
    34081 gttggcaccc cagttcgcca ttaccgaata cacatccgta aacggccctt taccagtgag
    34141 gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg cgtttgttga ggatgtcatg
    34201 cacatccttc ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg gtcccaggcc
    34261 tttgatgatg ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga
    34321 agagtaaccg ccgccacgga agtattcgtg gatcgccggg caccattcgg tagcggcgag
    34381 gcaagcgtca gcctcttgct gagagatttc ccagtgcggc ttcatcgtcg ggttaccttc
    34441 gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa cctgagttga tcaaatggat
    34501 gatgccgtgt tctgccagtc cgtccagttt atgccccgtt acacgctcaa ttgcttctgg
    34561 tgaccagtag gtacgcacat cggcaaatac ctgagcggtg ccggtgagct ggtgacccat
    34621 tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac
    34681 gccattccag tcaaatgaac tgttgaggat cgcttcggcg gtgtcaccat tgggatattg
    34741 atcggtccag tgacgttgcc cctggaagcc cgcagcgatg gcgttgtagc caagtgattc
    34801 ttccacgcga ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc ggatacacat
    34861 cgccatcagt aaactttcgc gcagaactgc gcggctttgc tcggcattgc gttgatactg
    34921 tttgttattt tcatcttcgc catagcggaa gtttttatca gcccaggcca gtgccatttc
    34981 cagttcggct tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg tcatatccac
    35041 cgcctggact ttcattccca gccaggattc aaagaagttg tgatcaacaa tagaaccggc
    35101 gatacccatc gaaacgccgc ccagcgacag atagctttta cctttcatgc tggcgacggc
    35161 caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc
    35221 atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg
    35281 agctgccaga gccgctgcca ggtaaacagc gccggggcgt tcagtgccgt taaagcccca
    35341 aatggctttc gggcgggttg gatccatgtc gatggtttca ctgccatagc accagcaagg
    35401 cgttaccgta atggtgaggc ctacattctg gctgctgaat ttttcttcgc aagcagcggc
    35461 ttcagccata cccgcgatac aggtatcgga aatgacacac tcgacggcag ctccgcaggt
    35521 atggcgcagt ttctcggtca gcagtgcggc cgtagctttc gccatattca ttgtttgttc
    35581 ttcaagcgac tcacgaacac ccatgcgacg accgtcaata accgggcgga taccaatttt
    35641 cggtaagctg atttttttca ttacagattc ctcactttat tcggaaaata tgttcagtta
    35701 gttgccgttt gagaacggaa acgggcaaag ataaagatga ccgcgaagca gagtgcgggg
    35761 atcagttcag cagtggggat gttgcccgcc gcgtcactga caaaacccat gaccggagtg
    35821 acaataccgc cgccaataat ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg
    35881 ccgagattct taatgcccag cgagaagatt gttgggtact gaatcgacat aaaggcgctg
    35941 cataaagtca gggctattaa gcccacatga ccgccagcga aggctgagat caggcacagt
    36001 gccatagcga ttaatgcgta ggcggccagg actttgtgtg gtgcgaagcg actgatgagc
    36061 caggtaccgg tgatacgacc aataaagaag cacaccatgg ttccggttaa atagttagcg
    36121 gcaaagcctg cagtcatacc tggaatttct tctacagcgt agcgaatcaa atagctccag
    36181 caggccgttt gtgcgccgac atagcagaat tgcgctaata ccgcccagcg ccagtgacga
    36241 atacgcgcca gacgagaaag cgatgcggag aacgatcctt gtttggcgtc actgtgatta
    36301 tcactctgca atgccgggaa tttcgtcagc atgatcagca gggcgaccag taacacgata
    36361 gccacgatga tcatataagg tgtctgtacc gataatacta ggctgtgttt atacgcactc
    36421 aattgctctg gagacatttt atcgagaacg tcttgcgatt gatgtggcac gttagacaaa
    36481 ataagacttt gcccaaagac aaccgcgata attgcgccaa acgagttaaa tgtttgcgca
    36541 agatttaagc ggaagtgact actgctttcc ggccctaata ccgtaacaaa agggtttgcg
    36601 gcagtttcca gacaacctaa tccggctgca ataataaata ggccaactaa aaataaggtg
    36661 tagttcatta tttctgcagc gggccagaat aatgcagcgc ccaaggcata taaaaataat
    36721 ccggtaataa tccctgcttt ataactgagt tttttcatca atatcccagc agggattggg
    36781 ataatgaaat aaccaaagta aaaggccgat tggatcaggc cagcctggaa atttgtcagc
    36841 gtaaaagcct gctggaattg aggtaataaa atgtcgttaa ggttattggc taccgcccaa
    36901 agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt aacttctgct ttgccctgca
    36961 tctttatcta ccgcacggta actctgcgtt tgtattgatg tgtttcccat agcatcctct
    37021 taggttcaga agcttaagca cgtaatcggt tatgttttca aaagtgcaaa accagcgagt
    37081 tcgggcttca tatcggtgaa tatttgcctg tctgaaatga actcatggat tcattttcaa
    37141 ataaaaaacg ctctgtaata tgacggcggt cacacttatt agctggaagg tgactttatg
    37201 tgactaccat cactttaata atcgaaattt catgcccgtt tcagaaaata aatcataaaa
    37261 aacacaaacc agatgaccgt attaattgtt tgtgccgaaa acgggaattt ctggcgaggg
    37321 gaaatggaaa aatgaccgtt ttgataaata tttttaagag cggtcatttt taac
//
LOCUS       DAJTYI010000022        37078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYI010000022 DAJTYI010000000
VERSION     DAJTYI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..822)
                     /gene="yhdJ"
                     /locus_tag="OO564_001124"
     CDS             complement(<1..822)
                     /gene="yhdJ"
                     /locus_tag="OO564_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6652328.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRK"
     gene            complement(908..1204)
                     /gene="fis"
                     /locus_tag="OO564_001125"
     CDS             complement(908..1204)
                     /gene="fis"
                     /locus_tag="OO564_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6652329.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1230..2195)
                     /gene="dusB"
                     /locus_tag="OO564_001126"
     CDS             complement(1230..2195)
                     /gene="dusB"
                     /locus_tag="OO564_001126"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6652330.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2524..3405)
                     /gene="prmA"
                     /locus_tag="OO564_001127"
     CDS             complement(2524..3405)
                     /gene="prmA"
                     /locus_tag="OO564_001127"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6652331.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3417..4868)
                     /gene="panF"
                     /locus_tag="OO564_001128"
     CDS             complement(3417..4868)
                     /gene="panF"
                     /locus_tag="OO564_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6652332.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4858..5100)
                     /locus_tag="OO564_001129"
     CDS             complement(4858..5100)
                     /locus_tag="OO564_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6652333.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5209..6558)
                     /gene="accC"
                     /locus_tag="OO564_001130"
     CDS             complement(5209..6558)
                     /gene="accC"
                     /locus_tag="OO564_001130"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6652334.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6569..7039)
                     /gene="accB"
                     /locus_tag="OO564_001131"
                     /gene_synonym="fabE"
     CDS             complement(6569..7039)
                     /gene="accB"
                     /locus_tag="OO564_001131"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6652335.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8017..8991)
                     /locus_tag="OO564_001132"
     CDS             complement(8017..8991)
                     /locus_tag="OO564_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6652336.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9143..11083
                     /gene="csrD"
                     /locus_tag="OO564_001133"
     CDS             9143..11083
                     /gene="csrD"
                     /locus_tag="OO564_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6652337.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11388..12431
                     /gene="mreB"
                     /locus_tag="OO564_001134"
     CDS             11388..12431
                     /gene="mreB"
                     /locus_tag="OO564_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6652338.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12497..13600
                     /gene="mreC"
                     /locus_tag="OO564_001135"
     CDS             12497..13600
                     /gene="mreC"
                     /locus_tag="OO564_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6652339.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13600..14088
                     /gene="mreD"
                     /locus_tag="OO564_001136"
     CDS             13600..14088
                     /gene="mreD"
                     /locus_tag="OO564_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6652340.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14097..14690
                     /gene="yhdE"
                     /locus_tag="OO564_001137"
     CDS             14097..14690
                     /gene="yhdE"
                     /locus_tag="OO564_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6652341.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14680..16149
                     /gene="rng"
                     /locus_tag="OO564_001138"
     CDS             14680..16149
                     /gene="rng"
                     /locus_tag="OO564_001138"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6652342.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16217..20017
                     /gene="yhdP"
                     /locus_tag="OO564_001139"
     CDS             16217..20017
                     /gene="yhdP"
                     /locus_tag="OO564_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6652343.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20173..21618
                     /gene="tldD"
                     /locus_tag="OO564_001140"
     CDS             20173..21618
                     /gene="tldD"
                     /locus_tag="OO564_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6652344.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVSLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21746..22675)
                     /gene="aaeR"
                     /locus_tag="OO564_001141"
     CDS             complement(21746..22675)
                     /gene="aaeR"
                     /locus_tag="OO564_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6652345.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22858..23061
                     /locus_tag="OO564_001142"
     CDS             22858..23061
                     /locus_tag="OO564_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6652346.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23069..24001
                     /gene="aaeA"
                     /locus_tag="OO564_001143"
     CDS             23069..24001
                     /gene="aaeA"
                     /locus_tag="OO564_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6652347.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24007..25974
                     /gene="aaeB"
                     /locus_tag="OO564_001144"
     CDS             24007..25974
                     /gene="aaeB"
                     /locus_tag="OO564_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6652348.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26066..26338
                     /locus_tag="OO564_001145"
     CDS             26066..26338
                     /locus_tag="OO564_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6652349.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26394..26657)
                     /gene="yhcN"
                     /locus_tag="OO564_001146"
     CDS             complement(26394..26657)
                     /gene="yhcN"
                     /locus_tag="OO564_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6652350.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27022..27492)
                     /gene="argR"
                     /locus_tag="OO564_001147"
     CDS             complement(27022..27492)
                     /gene="argR"
                     /locus_tag="OO564_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6652351.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27927..28865
                     /gene="mdh"
                     /locus_tag="OO564_001148"
     CDS             27927..28865
                     /gene="mdh"
                     /locus_tag="OO564_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6652352.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(28927..29994)
                     /gene="degS"
                     /locus_tag="OO564_001149"
     CDS             complement(28927..29994)
                     /gene="degS"
                     /locus_tag="OO564_001149"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6652353.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30084..31451)
                     /gene="degQ"
                     /locus_tag="OO564_001150"
     CDS             complement(30084..31451)
                     /gene="degQ"
                     /locus_tag="OO564_001150"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6652354.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31605..32003)
                     /locus_tag="OO564_001151"
     CDS             complement(31605..32003)
                     /locus_tag="OO564_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6652355.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32197..33324
                     /gene="zapE"
                     /locus_tag="OO564_001152"
     CDS             32197..33324
                     /gene="zapE"
                     /locus_tag="OO564_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6652356.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33543..33971
                     /gene="rplM"
                     /locus_tag="OO564_001153"
     CDS             33543..33971
                     /gene="rplM"
                     /locus_tag="OO564_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6652357.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33987..34379
                     /gene="rpsI"
                     /locus_tag="OO564_001154"
     CDS             33987..34379
                     /gene="rpsI"
                     /locus_tag="OO564_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6652358.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34774..35412
                     /gene="sspA"
                     /locus_tag="OO564_001155"
     CDS             34774..35412
                     /gene="sspA"
                     /locus_tag="OO564_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6652359.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35418..35915
                     /gene="sspB"
                     /locus_tag="OO564_001156"
     CDS             35418..35915
                     /gene="sspB"
                     /locus_tag="OO564_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6652360.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35958..>37078)
                     /gene="dcuC"
                     /locus_tag="OO564_001157"
     CDS             complement(35958..>37078)
                     /gene="dcuC"
                     /locus_tag="OO564_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6652361.1"
                     /translation="GGYARYMERIGASRAMVSLLSRPLKLIRSPYIILSATYVIGQIM
                     AQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVA
                     GMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLY
                     YAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDD
                     VQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMAL
                     VIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVV
                     VVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8947 a         9277 c         9786 g         9068 t
ORIGIN      
        1 ctttcttttt ttcactttcg ccagttcttc cgcggagtaa tgcgacgcga catccagccg
       61 tcgaagcccc attttgatgt actcgctgtt gatctcaata ccaatgaatt ttcgtccgct
      121 ggcgatggct acggcaccgg tagtaaagct accggcaaac gggtcgagaa cgatatcgcc
      181 tgggttggaa gaggcgagaa taatgcgttt cagtaaggct ttcggttttt gcgtcgggtg
      241 gttctcatat tcatccatca aataacgcac gcgtggaaaa tcccagacgt tacccggtac
      301 tttttgatga ttgtatggct gtggaggatt tttgcgataa tagatcaacg cgcgctgcga
      361 tccggttttg gcttctacca gaatagcatc accgttgaat gtgtagttct ttgcgtcttt
      421 caccatcatc aggatgggtt cgtacatgga gccgtagtgt tttttcgcct gtactccaga
      481 actgtcatat gaccagacga tgcgactttt gatggtaaaa agcttgcggc actggagatc
      541 gataaagggc atgttttccg tactgttcat aatgtacatg ctgccctgct ttttcagaac
      601 gcggtggcac tctgcaatca cttcaaacag ccagtcgata aacagatctt ctttccaggc
      661 ttcgatcaaa ccatcaaaat ttttaccgat gttatacggt gggtcggcaa agagcagatc
      721 gacgctttca gtaggtagct ttttaagttc ggcaagggca tcaccgtgaa taatggtctt
      781 agcttcatta ccaaaccggg tcggttcaca tcctgttctc atggtcactc cctttgtgac
      841 acctataaaa aaggcgcttc cctatgccga gtagcgcctt tttaatcaag catttagcta
      901 acctgaatta gttcatgccg tattttttca attttttacg cagcgtacca cggttgatgc
      961 ccatcatcag cgcagcacgg gtctggttac cacgggtgta ttgcatcacc atgtccaaca
     1021 ggggctgttc tacttcagcc agtaccagct catagaggtc attcacatcc tgaccattca
     1081 gttgagcaaa atagttcttc agtgcctgtt taaccgagtc acgcaggggt ttttgggtta
     1141 cctgatcctg agagttaacg gtagaaacgg tcagtacgtc agaatttacg cgttgttcga
     1201 acatagttct gtcagctctt tatttctgtt tacgcaaaat tttcgaagta tgcctccaac
     1261 gcctccagct gttcgctggc atcctcaatg gcgttgaatg tgcgccgaaa ctggtcattt
     1321 ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg caattcggta cccttttgcc
     1381 ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa gcaagcgctt aacctctgcc
     1441 aaaggcagcg ggggcagcaa ctccccagtg tccagataat gctggatttc ccgaaagatc
     1501 cagggtcttc cctgagctgc gcggcctatc atcagggcat ccgcccctgt atagtcgagc
     1561 acagctctgg ctttaagcgg gtcagtaatg tcgccattcg cgataaccgg aatggaaact
     1621 ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc cattgaacaa acaggcgcgt
     1681 gtacggccat gaatggtcag agcctgaatg ccacagtctt cagccagttg ggcaatctct
     1741 tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct tcagggtaac aggaacgtcc
     1801 actgcattga cgacctcggt aaggatcgat ttaacgacat ccgggtactg caagagggct
     1861 gaacctgcga gcttgcgatt cactttttta gccgggcaac ccatattgat atcaataatc
     1921 tgggcaccgc tttccacgtt aatacgtgct gcatctgcca tttctttcgg agcgctacca
     1981 gcaatttgca cggtgcgaat accgggttca tcaatgtgca ccatccgtaa acgagatttg
     2041 tcgctttccc aaacctgtgg gttagaagac atcatctcgg atactgtcaa tccggctccc
     2101 atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc cagccatggg cgctgcgatc
     2161 aggcgatttc tgagctgata ttgtccgatg cgcatgagtt aagaaatgac catactgtga
     2221 ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg aaaggccaaa ctttgaccaa
     2281 tcctctgaga tggatcaaag aattgcattt aaaatgagcg tggtgcgata attactcata
     2341 aaaatcatca tattagaaaa tagtgactaa aatttacact caaagaaatt tgagtaagtt
     2401 ctcaattttt ctttatgaat gaaaattttg gcacgcaaat ttgcgtaaat aatcggcaaa
     2461 tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc gtcaccctac ggcgatgcga
     2521 aggttaattc ttacgaccgg taatacggca ccactcttct ttttccacga ccgggtccag
     2581 tgcgaagcta tcggcataag cttcacaaac gctctctgcc tggcttgcca gaataccgga
     2641 aaggcccagc aaaccgcctg aaaccggcag gacgctgatt aacggtgcca gttcacgtaa
     2701 tgggcctgca aggatgttag cgaccaccac gtcggctttc atttcttctg gctgatcttt
     2761 cggtaagtag agttccagac ggtcagaaac gccattacgt tcggcgttat cgcggctggc
     2821 ctgaatcgcc tgcggatcga tatcaatacc aatggctttt gctgcaccca gtttcagcgc
     2881 cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg actgttttac cggttaaatc
     2941 gaggctgtcg agccattgca ggcacagaga ggtggttgga tgggtacccg taccaaacgc
     3001 cagccctgga tctaacatca cgttgacggc gttttcgtcc ggcacatcac gccagctagg
     3061 gcagatccac agtcgttcac caaagcgcat cgggtggaaa ttatccatcc attcgcgctc
     3121 ccagtcttta tcttctagtt gttcggtttt atgcgcgaag cctgcgccga gcagcggatg
     3181 gttttccaga atcgccacca cgtcgttcat atcggtttca gcgtcgaaca gaccaatcac
     3241 atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc tcaaatactg gcgtatcgtg
     3301 ggtatcctga aaagtgatag aaacggcacc cgcttccatc agcgcatcgc taagatcttc
     3361 cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa ggcatggcaa aactctttat
     3421 ttatcagtag tcaaaacggt agcttgcggg acggatgtac cgaaacggtt tccgaccagg
     3481 aaagccagca aacttagtag taacgagggc acgatagggt ggaagcccag gtactgaata
     3541 ttcagcgtcg cgagtacggc atacagcacg ccgccaacga tcatcgcact tagcgcgcct
     3601 ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg gccacaggaa aacggcttcc
     3661 agcccaccga aggccagcaa attcagccag atgatcattt ctggcggctt ccaggcggca
     3721 agcagcagca acgcgccgag aactaacgta attaccgccg acatccgctt cagacgcgtc
     3781 tcgttttgca tttgatccgg acggatattc agatagagat ctttaatgat cgtagcggaa
     3841 ctttgcagca gttgggcgtt aattgtcgac atgatcgcag ccataggtgc agccagaaag
     3901 atcccggcag caaaaggtgg cagcactttt accattaacg tcgggatcac cagatccggt
     3961 acggtgagat cggggatcac tgcacgacct aacgctccgg ctaggtgcat accgaacatc
     4021 aggattgcga ctacaatcgt accgatgatg atcccccggt gtactgcttt gctgtcttta
     4081 taagagatac agcgcaccgc agtatgcggc aggccaatca cgccaaagca caccagtacc
     4141 cagaacgacg tcataaaggc aggcgacaga atatcgtcag cgccttgtgg cgtaaccagt
     4201 tgcggatcga tggtttgcaa ggtctgtact gcgttgctta agccgccagc ggcatgtacc
     4261 acgccaataa gcagcacaac ggtgccaatc agcatcacaa gcccttgcat ggtgtcgttc
     4321 agcacgctgg cgcgaaagcc accaaaggcg gtatataacg caatgctgat accaaaaatc
     4381 agcagcccgg tttcataagg aatacccgcc gcggtttcca gcaggcgcgc accgccgata
     4441 aactgcacgg tcatcgcacc aacgaacgca accagcaaac tcaaactcgc cagccacacc
     4501 agaagacgac tctggtagcg ggcaaacagc atatcgttca gcgtcactgc attgtagcgg
     4561 cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg aaagccagac agcaggaagc
     4621 tgaatcatcg ccagcaatac ccagcccagc ccgtatttat aagcagctcc tggcccgccg
     4681 ataaacgaac tggcactgat ataggtcgcg gtgagcgtca tcgccagcac aataccgccc
     4741 atagagcggc tgccgaggaa atactcatta aggaaggtgc cggtgctccg tttacgcatc
     4801 gcataaaccg agataccgaa caccaccacc agataggcga ccagcggtag aattacttca
     4861 agctgcatcg tcatcctcca gtgggatatc gcgatagata aatttcacca tcgcccagca
     4921 cagtccaata aacagtagcg gcgtcaggat gcaggccatc tcaaaccagc gcggaaagcc
     4981 ggtaaaaccg ggggcaacgc caggtaagta agcatctact aaccaaactg ccagatacaa
     5041 aagggtcagc cccagcgccc agcgcgcctc tttatgggcc tgaacaaaac tagtgtccat
     5101 tttttgtccc tgatgggtga agaaagcgag gattgtacct tatgggggtt gtcgatcccc
     5161 agtaataaaa aaggccggaa aatccggcct tttgacgctt ttgcagtctt atttttcctg
     5221 aagaccgagt tttttctcca gatagtggat gttagtgcca ccatgctgga agttctcgtc
     5281 attcatgatg cggatctgca gatcaacgtt ggttttgata ccgtcgatga tcagctcctg
     5341 cagcgcattc ttcatgcggg caatcgccac gtcacggttt tcaccgtagc aaatcagctt
     5401 accgatcatt gagtcatagt acggcggtac ggtgtagccc gcgtagatat gagactccca
     5461 acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt ttgcctgggc ttggcaggaa
     5521 ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca tggccgcgaa cgtgaacttc
     5581 ttcttgcttg atcgacagcg gttgaccggc agcgatacgc agctgttctt tgatcaggtc
     5641 aacgccggtg atcatttctg taaccgggtg ttctacctga atacgggtgt tcatttcgat
     5701 gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta cctgcaccgc gatagccgat
     5761 atcaacacac gctttagcgc aacgttcgcc gatgtagcga cgcagttccg gggtaatgcc
     5821 cggtgctggt gcttcttcga ccactttctg gtggcggcgc tgcatggagc agtcacgttc
     5881 cgccagatag atagcgttgc cctgaccgtc agccagtacc tgaatctcga tgtggcgagg
     5941 attctccagg tatttctcca tgtaaaccat atcgttgctg aaagcagctt tcgcttccgc
     6001 acgggtcatg gagatggatt gtgccagttc agcgtcgccg cgcactacgc gcataccgcg
     6061 accgccgccg ccgccggagg ctttgataat caccggataa ccaatgcgtt tagcaatggc
     6121 gcggttttta tccatatcgt cgcccagcgg gccgtcagaa ccaggtacgc aaggaacgcc
     6181 cgcttttttc atcgccgcga ttgcggatac tttgtcgccc atcaggcgaa tggtttctgc
     6241 tttcgggccg atgaagataa agccggagcg ttcaacctgc tcggcaaagt tggcgttctc
     6301 ggagaggaag ccgtaacccg gatggattgc tactgcgccg gtgatttcag cggcgctgat
     6361 gattgccggg atgttcagat aactttgtac tgacggagca gggccaatac agaccgtttc
     6421 atctgccagt aatacgtgtt ttagatcgcg atccgcgctg gagtgcacag cgacagtctt
     6481 gatgcccagt tctttacagg cacgaagaat acgcaatgca atctcgccgc ggttggcaat
     6541 aacaatttta tccagcatgt tcgcctcgtt actcgatgac gaccagcggc tcgtcaaatt
     6601 ctaccggttg tccactttcg accagaattg ctttcacggt accggatttg tccgcttcga
     6661 tctggttcat cattttcatg gcttcaacga tgcacagggt atcgcccacg ttgactttct
     6721 gacccacttc gatgaacgct tttgcgtccg ggcttggggt gcggtagaaa gtaccaacca
     6781 tcggggaacg tacgatgtga ccactgattt ccgctgctgc tggcgcttcc atggaaggaa
     6841 cggtcgccgg agcggctgcg ttagattgag ctggctgctg catcattggt gcagcgtaag
     6901 cttgttgcat cacagggaaa cttgcggcag gagctgcacg gctaatgcgt actgactctt
     6961 cgccttcaga aatttccagt tcggagatgc ctgattcttc aaccagctcg atcagttttt
     7021 taatcttacg aatatccatg agtgggttcc gtactctttg tttagtgtga ttgtgacagg
     7081 cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg tatgcatagc gataaattcc
     7141 agcaggccgg gacgccggtc tattttgccc cagagtgtca atgttagact tgacggacat
     7201 tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa tataccttcg ttgcgccaat
     7261 gtcacctttt ccgcagcgca aaaactgcgt cagggaggac gaggtcgcac attataacga
     7321 tttcgtagca attggcagct aaatactggt cttatcaggg aagataatca acagctaaca
     7381 tgtaaataac cttcaacacc gtgtaatttg caacaagccg cacaattcac gaaattagcg
     7441 ccatcatcga cggaacttct tataacgtaa ggtgaaaagc tgaaaagcca gccctgcgaa
     7501 gatgaggggc tgcaatgaga taatcttcac agaccacaaa taatgtaggg atgccaggat
     7561 cgcgacaaga tagacgacat tacgcagcgg ttgctgggga ttacccaatt ttcactgcat
     7621 cgtctggata gatgtgaatg ctaaagcacg caaaatcaac caactgacaa ttctcagcat
     7681 taaataggtt cgagtggtta atttgtagtc aaataaaatc agattattca ccccatggta
     7741 ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc aaagcaccat aaacctaaca
     7801 ggcgacggag gcgtatcaat aatggctgtt tagaggagta tcaatgcgca tcaatttgtg
     7861 gtcagtcgaa atcatgactt tccagtttgg atgatgttgt acaacttcct gaccaaggtg
     7921 ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac cgattgaata atgatgtcaa
     7981 aatgagttcc ctctcttatt attccttgct aaatggttag ttaaccttca ccagcgtgcg
     8041 accttggatc tggttattaa tgatggcctc ggcaaagttc ggtgcctctg acagagatat
     8101 ctcttttgcc gcctgggtat agaatgattc cggtaaatcg gcgaccagtc gctgccaggc
     8161 ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc ccttgcaaac ggacattacg
     8221 cagaataaat ggcatgaccg tgcttggcag agtaaaacca cccgccagac cacaggccgc
     8281 cacgcagccg ccgtaattca tttgcgccag cacttttgcc agcactttgt cgccaacagt
     8341 gtcaattgcc ccagcccaga cttgtttttc cagaggacgg gattcggcaa actcatcacg
     8401 agggagaata cggctggcac ctaaactttt cagatattca tgggtacttt cgcgaccgga
     8461 aacggcaacg acctgataac ccaacttatg cagcagcgcc acggcggtac tgccgacgcc
     8521 accactggca cccgtcacga caatctcccc gtcctgcggg cgaacaccgg catcttccag
     8581 cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg ataatcattg ctttacgcgc
     8641 gtccagccct tgcggcatgg caaccagcca gtcacctttc actcgcgcct gctccgccag
     8701 cccaccccag tggttttcac caacgcccca gccagtgagt aacacctcct gaccggcatg
     8761 aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa tcgatcccag gaatcatcgg
     8821 aaaattacgg atgatttttc ccttaccggt aatcgccagc gcatctttat agttcaggct
     8881 cgaccagtga acatcgaccg tgacatcgcc ctccggcagg cgactttcgt ccagagtctg
     8941 tactgatgcg agagttttgc cgtcctgctg ttctaaaagt aacgcctgca taagtggtcc
     9001 tcatgtgcat gatggattgg aaaataattt ctgaagacta tactcgctaa tggaaaagca
     9061 aagccgatga agcgcaataa attgcgagat aaatctgatt tgctagtatg cccgcttcct
     9121 cactatcgga gttaacacaa ggatgagatt aacgacgaaa ttttcggcct ttgttacgct
     9181 gctcaccggg ttaacaattt ttgtgacttt gctgggctgt tcgctaagtt tctacaacgc
     9241 cattcagtat aagtttagtc atcgcgttca ggcggtggcg acggcgattg atacccacct
     9301 tgtgtcgaat gacttcagcg cattaaggcc acaaattacc gaattaatgg tgtcagcaga
     9361 tatcgttcgt gtagacctgc tgcatggtga taaacaggtt tataccctgg ccagaaatgg
     9421 tagttatcgt ccggttggca ccagcgatct gtttcgtgaa ctgagcgttc cgttgataaa
     9481 gcatccgggg atgtcgttgc gtctggttta tcaggatccg atgggcaact atttccattc
     9541 gttgatgacc accgcgccgc tcacgggggc gattggcttt atcattctta tgctctttct
     9601 ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa ttgctggaaa cccgggctac
     9661 tcgtatctta aacggtgagc gtggctctaa tgtgttggga accatctatg aatggccgcc
     9721 cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt cagaacgcac gcgaacaaca
     9781 cagccgtctt gatacgctga tccgctctta tgccgcacag gacgtgaaaa ccggcctcaa
     9841 taaccgactc tttttcgata atcagttagc aacgttactg gaagatcagg agaaagtagg
     9901 tacccacggg atcgtgatga tgattcgtct gccggatttc aatatgttga gcgatacctg
     9961 ggggcacagc caggttgaag aacagttctt cactctgacg aatctgctgt cgacatttat
    10021 gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc agtgattttg ctgcgctgtt
    10081 accgcaccgg acgttaaaag aggcagagag catagccagt cagttaatca aagccgtcga
    10141 taccttgccg aacaataaaa tgctcgatcg cgacgatatg atccacattg gtatctgtgc
    10201 ctggcgtagt ggtcaggata ccgagcaggt aatggaacat gcagagtctg ccacgcgtaa
    10261 tgcgggattg cagggtggca atagctgggc tatttatgat gactcgttgc ctgaaaaagg
    10321 acgcggtaat gttcgctggc gtacgcttat cgagcaaatg ctcagtcgcg gcggcccgcg
    10381 cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt catcatcgcg aactcatgtg
    10441 ccgcatcttc gatggtaatg aagaggttag ctcggcggag tatatgccga tggtcttgca
    10501 gtttggctta tcggaagagt atgaccgtct gcaaatcagc cgtcttattc cactattgcg
    10561 ttactggcca gaggaaaatc tggcgattca ggttaccgtt gagtcgctga ttcgtccgcg
    10621 ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa aaatcacaac gaaaacgcat
    10681 aattattgaa cttgcagagg ccgatgtagg tcaacatatc agtcgtttac aacctgttat
    10741 tcgtttagtg aatgctttag gggtacgggt agccgtcaac caggctggtt tgacgctggt
    10801 aagcaccagt tggatcaaag aacttaatgt tgagttactc aagctccatc cggggctggt
    10861 caggaacatt gagaagcgaa cggagaacca gctgctggtt caaagcctgg tggaagcctg
    10921 ctccgggacc agcacccacg tttacgccac cggcgtgcgt tcgcgaagcg agtggcagac
    10981 cctgattcag cgcggtgtta caggcgggca aggggatttt ttcgcgtcct cacagccact
    11041 tgatactaac gtgaaaaaat attcacaaag atactcggtt taacctgccg tttaatccgt
    11101 tttcacgtag aataacgcgc gctgcgtctc atgggagtgt gcttgtctgc tcgccagatt
    11161 gttgcagcac atatgcagat gaatgacctt acgcggttgc aaacaggcga ggaatgctgc
    11221 tgatgcatta agcctttctg gactcaggca gagatttgta acaaaggaaa cgaactgcac
    11281 taattttcac cgtagcagat gatttttgcg ccttgtcgct gctgcgtgtg gttggtaaag
    11341 taagcggatt ttcttttccg ccccagcttt caggattatc ccttagtatg ttgaaaaaat
    11401 ttcgtggcat gttttccaat gacttgtcca ttgacctggg tactgcgaat accctcattt
    11461 atgtaaaagg acaaggcatc gtattgaatg agccttccgt ggtggccatt cgtcaggatc
    11521 gtgccggttc accgaaaagc gtagctgcag taggtcatga cgcgaagcag atgctgggcc
    11581 gtacgccggg caatattgct gccattcgcc caatgaaaga cggcgttatc gccgacttct
    11641 tcgtgactga aaaaatgctc cagcacttca tcaaacaagt gcacagcaac agctttatgc
    11701 gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc gacccaggtt gaacgccgcg
    11761 caattcgtga atccgcgcag ggcgctggtg cccgtgaagt cttcctgatt gaagaaccga
    11821 tggctgccgc aattggtgct ggcctgccgg tttctgaagc gaccggttct atggtggttg
    11881 atatcggtgg tggtaccact gaagttgctg ttatctcctt gaacggcgtg gtttactcct
    11941 cttccgtgcg cattggtggt gatcgtttcg acgaagctat catcaactat gtgcgtcgta
    12001 attacggttc tctgatcggt gaagccaccg cagaacgtat caagcacgaa atcggttcgg
    12061 cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg ccgtaacctg gcagaaggtg
    12121 ttccacgcgg ttttaccctg aactccaacg aaatcctcga agcactgcag gaaccgctga
    12181 ccggtattgt gagcgcggta atggttgcac tggaacagtg cccgccggaa ctggcttccg
    12241 acatctccga gcgcggcatg gtgctcaccg gtggtggcgc actgctgcgt aaccttgacc
    12301 gtttgttaat ggaagaaacc ggcattccag tcgttgttgc tgaagacccg ctgacctgtg
    12361 tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat gcacggcggc gacctgttca
    12421 gcgaagagta atcggatgca ggcaggggaa gcgtctgttt accctgcctg gtctgatacg
    12481 agaatacgca taacttatga agccaatttt tagccgtggc ccgtcgctac agattcgcct
    12541 tattctggcg gtgctggtgg cgctcggcat tattattgcc gacagccgcc tggggacgtt
    12601 cagtcaaatc cgtacttata tggataccgc cgtcagtcct ttctactttg tttccaatgc
    12661 tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc tcgcgtgacc aactagaact
    12721 tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac agtgaactgc tgatgcttgg
    12781 acaatacaaa caggagaacg cgcgtctgcg cgagctgctg ggttccccgc tgcgtcagga
    12841 tgagcagaaa atggtgactc aggttatctc cacggttaac gatccttata gcgatcaagt
    12901 tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc cagccggtca tcagcgacaa
    12961 aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc agtcgcgtgc tgctgatttg
    13021 tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac gatatccgcg taattgcagc
    13081 cggtaacggt tgtacggatg atttgcagct tgagcatctg ccagcgaata cggatattcg
    13141 tgttggtgat gtgctggtga cctccggtct gggcggtcgt ttcccggaag gctatccggt
    13201 cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct tatactgtga tccaggcgcg
    13261 tccgactgca ggactgcaac gtttgcgtta tctgctgctg ctgtggggcg cggatcgtaa
    13321 cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt gctaatgaac gtctgatgca
    13381 gatgatgccg caggtattgc cttcgccaga cgcgatgggg ccaaagttac ctgaaccggc
    13441 aacggggatc gctcagccga ctccgcagca accggcgaca ggaaatgcag ctactgcgcc
    13501 tgttgcgccg acacagcccg ctgctaatcg ctctccacaa agggctacgc cgccgcaaag
    13561 tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag tggcgagcta tcgtagccag
    13621 ggacgctggg taatctggct ctctttcctc attgcgctgt tgctgcaaat catgccctgg
    13681 ccggataacc tgattgtttt ccggccaaac tgggtgttac ttatcttgtt gtattggatc
    13741 ctggccttgc cccatcgcgt aaatgtgggc acaggttttg tgatgggtgc catactggat
    13801 ctgatcagcg gctcgatgct tggcgtacgc gtattggcga tgagcatcat tgcttacctg
    13861 gtggcgctga aataccagct tttccgcaac ctcgcattat ggcagcaggc gctggtcgtc
    13921 atgttgcttt cgctggtggt ggatattatt gttttctggg cagagttttt agtgattaac
    13981 gtctctttca gaccggaagt gttctggagt agtgtagtca atggggtgct ctggccgtgg
    14041 attttcttgc tgatgcgtaa agtccgtcag cagtttgccg tgcaataaag gtttctatga
    14101 cttctctgta tttagcttcc ggttctccgc gtcgtcagga gttacttgcg caacttggcg
    14161 tgacctttga acgtattgtt acgggcattg aggagcagcg tcagccgcag gagagcgcgc
    14221 agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc gggtgtcgcg caaacggcac
    14281 aggatctccc ggtgctgggt gcggatatta tcgttatcct gaacggagaa gtgctcgaga
    14341 aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa attatcgggt cagacccatc
    14401 aggtgatgac ggcagtggcg ttggccgaca gccagcatat tctcgattgc ctggtggtca
    14461 ccgatgtgac tttcagaacg ttaacagacg aagacatcgc gggctatgtc gccagcggtg
    14521 aaccgttaga taaagcaggt gcatacggta ttcaggggct gggtggctgt tttgtcagga
    14581 agataaatgg cagctatcac gccgtagtcg gcttaccgct ggttgaaacg tatgaattat
    14641 taagtaattt taacgcactg cgtgagaaaa gggataaaca tgacggctga attgttagta
    14701 aacgtaacgc cttcggaaac gcgagtggcg tatattgatg gcggtattct gcaggaaatt
    14761 catattgaac gtgaggcgcg acgcggaata gtaggcaata tctacaaggg tcgtgtaagt
    14821 cgtgtacttc cgggtatgca ggcggctttt gtagatattg ggctggataa agccgcgttt
    14881 cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg cgggtgaaga acaaaagcaa
    14941 ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg
    15001 gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca ccgatatcac gctgccttct
    15061 cgctatctgg tatttatgcc aggggcttct cacgttgggg tttcccaacg aattgaaagc
    15121 gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg
    15181 tttatcatcc gcactgcagc ggaaggggtt ggcgaggctg aactggcctc cgatgccgct
    15241 tatctgaaac gcgtctggac caaagttatg gagcgtaaaa agcgcccgca gacccgttat
    15301 cagctgtacg gcgaactggc gctggcgcag cgtgttctgc gtgattttgc cgacgccgaa
    15361 ctggaccgca ttcgcgttga ctcacgcctg acttacgaag cattgctgga gtttacctcg
    15421 gagtacattc ccgagatgac cagcaagctg gagcattaca ccggacgcca gccgattttc
    15481 gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg aacgcaaagt agaactgaaa
    15541 tccggtggtt atctgattat cgaccagacc gaagcgatga ccaccgtgga catcaatacc
    15601 ggagcgtttg tcggtcatcg caatctggac gacactattt tcaataccaa tattgaagcg
    15661 acgcaggcta tcgctcgcca gttacggttg cgtaatctgg gcgggattat cattattgat
    15721 ttcatcgata tgaataatga agatcaccgc cgccgcgtgc tgcattcgct ggagcaggcg
    15781 ttgagcaaag accgggtgaa aaccagcgtt aatggttttt cggcgctggg gctggtggag
    15841 atgacgcgta aacgcacccg cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc
    15901 tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag
    15961 attgttcgtg tccaccatgc ttacgactcc gaccgtttcc tggtctatgc ttctccggca
    16021 gtagccgaag ccttgaaagg cgaagagtca cactcgctgg cggaagtgga aattttcgtt
    16081 ggcaaacagg ttaaagtaca aattgaaccg ctctataacc aggagcagtt tgacgtcgta
    16141 atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt ttttgagtca catttttagc
    16201 agacaaggag tgacgggtga ggcgattgcc ggggatttta ctgcttactg gagccgcgct
    16261 cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt gctttaccgc atcttgacgc
    16321 ctggcgtccg gaaatcctca acaaaataga atccgcgact ggcatgccgg tagaagccag
    16381 tcagctctca gccagctggc agaattttgg cccgacgctt gaagcacacg atatccgtgc
    16441 agaactaaaa gatggcggcg aattttcggt taaacgcgtt actctggcgc tggatgtctg
    16501 gcagagcctg ttacatatgc gctggcagtt tcgcgacctc actttctggc agctgcgctt
    16561 tcgcaccaac actcctatca ccagcggtgg tagcgatgac agtctggaag ccagtcacat
    16621 cagcgatctg tttcttcgtc aatttgacca tttcgatctc cgcgacagtg aagtcagttt
    16681 tctgacgcca tccggtcaga gcgccgagct ggcgatccca caactcacct ggctgaacga
    16741 tccacgtcga caccgtgcgg aaggcctggt aagcctctcc agccttaccg ggcagcacgg
    16801 cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg ttgttaagca atggtcgcgt
    16861 ctggctacag gcggatgaca tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa
    16921 tattgctctg gaaacggcac agttctccct tgaaggctgg atgacgatcg acaaaggcga
    16981 tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc agctggttgg gcgagaagca
    17041 aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt acgcgtgaaa atccgggctg
    17101 gcagttctct attcccgata cacggatcac gatggacggc aaaccctggc cgagcggagc
    17161 attgacgctg gcctggatac cggaacagga cgttgacggc aaagacaata aacgcagtga
    17221 cgaactccgg attcgcgcta gtaatctgga gctggcaggc ttggagggca tacgcccgct
    17281 ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc tccacacaac cgagcggcaa
    17341 gattaacact ctggcgctgg atatcccgct tcaggcggca gacaagaccc gttttcaggc
    17401 atcgtggagc gatctggcct ggaagcaatg gaaattatta ccgggtgcgg aacacttctc
    17461 cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa
    17521 gatgccttac gaaacggtat tccgtgcgcc actggaaatc gccgacggcc aggcaactat
    17581 aagctggctg aacaatgaca aaggtttcca gctggatggg cgtaatattg acgttaaagc
    17641 caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa cctgctaacg atgaaccctg
    17701 gctgggtatt ctggctggca tcagtaccga tgatggttca caagcctggc gctatttccc
    17761 ggaaaacttg atgggtaaag acctggttga ttatttaagt ggtgcgattc agggcggtga
    17821 agcggataac gcgacgctgg tttatggtgg caatccgcaa ctcttcccct ataaacacaa
    17881 cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg aagtttgcct tccagccgga
    17941 ctggcctgca ttaactaacc ttgatattga actggacttt attaacgacg gtttatggat
    18001 gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc
    18061 tgactactca aaagaaaaac tgctgattga cgctgacatt aaaggtccgg gtaaagccgt
    18121 tggcccttac tttgatgaga caccgctgaa agattctctg ggtgcgaccc tgcaagaact
    18181 ccagctcgac ggcgatgtga atgctcgctt acatcttgat atcccgctga acggcgaact
    18241 ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt ctgtttatca aaccactcga
    18301 cagcatcctg aaaaatttga gcggtaaatt cagctttatc aatggcgatc tgcaaagtga
    18361 accactgaca gcaagctggt ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg
    18421 ggcaaaagcc taccaggttg cggtgaatct caacggtaac tggcaaccgg cgaaaaccgg
    18481 cgttctgcct gaagcggtga acgaagcatt gagtggcagc gtggcgtggg atggtaaagt
    18541 gggcattgat ctgccttatc atgctggcgc gacctataac gtagagctga acggcgatct
    18601 gaataatgtg agtagtcact taccttcacc gttagccaaa cctgcgggtg aaccactagc
    18661 ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa ttaaccggac aggctggtgc
    18721 ggataaccat ttcaatagcc gctggttgct tggtcaaaag ctgacgctcg atcgtgctat
    18781 ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa caaagtggcg ttgaacttaa
    18841 tatgccgccg atgaatggtg ccgagtggct ggccctgttc cagaaaggcg ctgcggagag
    18901 tgtcggtggt gcagcgagtt tcccacaaca cataacatta cgtacgccta tgttgtcact
    18961 gggaaatcag caatggaata acctgagtat tgtttcgcaa ccgacggcaa atggcaccct
    19021 ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg atgcgtaata acgcgccgtg
    19081 gctggcgaat atcaaatatc tttattacaa cccgagcgtg gcgaaagctc gtggtgattc
    19141 aacgccgtca tcacctttcc cgacaacgga gcgcattaac ttccgtggct ggccggacgc
    19201 acaaatacga tgcacagagt gctggttctg ggggcaaaaa ttcggtcgca ttgacagtga
    19261 tatcaccatt tctggcgata cgttaacgct gaccaatgga ctgattgata ctggtttctc
    19321 gcggcttact gccgatggtg aatgggttaa taatccgggg aatgaacgta cctcgctgaa
    19381 aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc
    19441 catacgccag tcgtcattta atgtggatta cgatttacac tggcgcaaag caccctggca
    19501 gccagatgaa gcgacgttga atggcatcat tcatactcaa ctgggtaaag gcgaaattac
    19561 cgaaatcaat accggacatg ccgggcaatt gctgcgctta ttgagcgtag atgccctgat
    19621 gcgtaagctg cgttttgatt tcagagacac ttttggcgaa gggttctatt ttgactccat
    19681 tcgcagcacc gcgtggatta aagacggcgt tatgcacacc gacgacacgc tggtggatgg
    19741 cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg gtacgtcgag acctgaatat
    19801 ggaagcggtt gtcgcaccag agatttctgc gacggtgggc gtggctgcgg cttttgcggt
    19861 taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa gtgctggggc cgctgtggag
    19921 caaagtctcc attttgcgct atcacatttc gggtccgctg gatgatccgc aaatcaacga
    19981 agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc
    20041 ccaatctcat atgataatcg ttgccaaagg ccaacgagcc ggaacataac tgtaggccgg
    20101 atagggcgtt cacgccgcat ccggcagccg taaaaaatcc tctactgcag taactaacga
    20161 gtagcaaaaa cgatgagtct gaacctggta agtgaacaat tgctagcggc gaacggcctg
    20221 aaacatcagg acttgttcgc gatcctcggt caactggccg aacgtcgcct tgattatggc
    20281 gatctctatt ttcagtcgag ctatcacgaa tcctgggttt tagaagaccg cattattaaa
    20341 gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc
    20401 ggatttgctt acgctgacca aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg
    20461 cgcaccatcg tccgtgatag tggtgatggc aaagtacaga cgctgggcgc ggtagagcat
    20521 agcccgttgt atacctcggt agatccgctg caaagcatga gccgtgaaga gaagctggat
    20581 atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact
    20641 gccagcctca gtggtgtcta tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg
    20701 gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa
    20761 cgcgaacgcg gtgccagtgg cggcggcggt cgttttagtt atgagttctt ccttgccgat
    20821 cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc
    20881 agtctttctg ccgttgctgc accagcgggc accatgccgg tagtacttgg cgcaggttgg
    20941 ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg aaggcgactt caaccgccgt
    21001 ggcacttcag tatttagtgg acaggtcggg gagctggtgg cttcagaact gtgtaccgtg
    21061 gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg cgattgatga cgaaggtacg
    21121 ccaggccagt acaacgtgct gattgagaac ggcattctga aaggctacat gcaggataaa
    21181 ctcaacgcgc gtttgatggg gatgacgccg actggcaacg gtcgccgtga atcctacgcc
    21241 catctgccca tgccgcgtat gaccaacacc tatatgctgc cgggtaaatc gaccccgcaa
    21301 gaaattattg aatccgttga gtacggtatc tatgcaccga actttggtgg cggtcaggtg
    21361 gatatcacct ccggcaaatt cgttttctcc acttcagaag catatctgat tgaaaacggt
    21421 aaagtaacga agccggtgaa aggcgcaacg ttgattggtt ccggtatcga aaccatgcag
    21481 cagatttcga tggttggcaa cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa
    21541 gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt tgaaagtcga taacctgact
    21601 gttggcggta ctgcgtaata atttcattat tttcagaagg ataattaatc tttctacgtg
    21661 cacgaacggt cccctcgctc ctctggggtt agggtgaggg gaacccgttg gcacaggttt
    21721 gtagaacgta acagtacaat atgaattact tctctttccc gcgcccgtgc atctcctgaa
    21781 acaatttacc gacctcaaca aaataatccg tcagcgagtt gatcacgacc tgtaccttca
    21841 gcggcagctt atctttttcg gtatataacg cataaaccgg gcgtggatct gactgataac
    21901 gcggcagcag gatctccagc tccccacgat tgatctcgtt gatcacccac atcagcggca
    21961 cgtaggcgat cccggcaccc gccgttagcc agcgcaccag cgtcatcgga tcattagtca
    22021 caaatctccc ttgcgggatc aggcgagtcg agatcccttc cggtgcaatc agttcaaatt
    22081 cattgtcggg ccgcacgctg tattcaagcc atgaatggct acttaaatcg gcgggttttt
    22141 ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca caccaccatt ggcatcgcgc
    22201 caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc gacgcggatc accacatcca
    22261 gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac cagattggcg ctcaaacctg
    22321 ggtattcttt cagcattttg gctgtcagcc cggcgagaac attttgtgcc atagttgaag
    22381 aacagccaat gcgtagcgtc ccgatggggg tgttattgaa ggcatacagt tgctcatgaa
    22441 catcctgcac ttcatgaagc atacgacggc agccctggta gtaaattcta ccggcttcgg
    22501 tcaggccaat gctgcgtgtg ctacggttta acagctttac ctgcaactca tcttccagtt
    22561 ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa
    22621 aagagccaaa ttcaactact ttggcaaaca ccgacatgcg ttttagtcgt tccattattc
    22681 actctgactt aaaagtgatt tagatcacat aatatagata acagcataac agttacgcta
    22741 atatattaaa tatcaatcta cagcaatgtt gctctcgccc ggctttccat gcccttctct
    22801 gcggcgacag atgctgaaaa taataacgcc tgctctctct atacaaccaa ggtcaacatg
    22861 agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc caccgatatt ttttgaattg
    22921 cttttatcac tggcgatttt ctggctggtg cgccgggtac ttgtgccaac aggtatctac
    22981 gactttgtct ggcatccggc gttgttcaac accgcgctct attgctgctt gttttatttg
    23041 atatcgcgac tgttcgtttg aggttgaagt gaaaacacta ataagaaaat tctcccgtac
    23101 ggccatcacg gtcgtattag tcattctggc cttcatcgca atttttaatg cctgggtcta
    23161 ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc gctgacgtcg ttgcgatcgc
    23221 gccggacgtt tctggactca ttacccaggt gaatgttcat gataaccagc tggtgaaaaa
    23281 aggacagata ctgttcacca tcgaccagcc gcgctatcaa aaggcgcttg aggaagcgca
    23341 agccgatgtt gcttattatc aggtactggc acaggagaaa cgccaggagg ccggacgtcg
    23401 taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc gaccaggcta acaacgtact
    23461 acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc cgcgatctgg caaaactgga
    23521 tcttgaacgc acggtgatcc gagcgccagc agatggctgg gtgaccaacc tcaacgtcta
    23581 taccggtgag tttattactc gaggatcaac ggcggttgcg ctggtgaaac agaactcctt
    23641 ctatgtactg gcctatatgg aagaaactaa gctggaaggg gtgcgtccgg ggtatcgtgc
    23701 agagatcacg ccgcttggca gtaacaaagt gctgaaaggg actgttgata gtgttgccgc
    23761 tggggtcacc aacgccagca gcacgcgtga cgacaaaggg atggcgacca tcgactctaa
    23821 ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt attcgtctcg acaaccagca
    23881 agagaacatc tggcctgcgg gcaccactgc tacagtggtg gtcactggca aacaagatcg
    23941 cgacgaaagc caggattcgt tcttccgtaa aatggcccat cgcctgcgtg agtttggtta
    24001 atcacgatgg gtattttctc cattgctaac caacatattc gctttgcggt aaaactggcg
    24061 accgccattg tactggcgct gtttgttggc tttcacttcc agctggaaac gccacgctgg
    24121 gcggtactga cagcggcgat tgttgccgcc ggtccggcct ttgctgcggg aggtgaaccg
    24181 tattctggcg ctattcgcta tcgtggcttt ttgcgcatca tcggcacatt tattggctgt
    24241 attgccggac tggtgatcat cattgcgatg atccgcgcac cattattgat gattctggtg
    24301 tgctgtatct gggccggttt ttgtacctgg atatcctcgc tggtacgaat agaaaactcg
    24361 tacgcgtggg ggctggccgg ttataccgcg ctgatcattg tgatcaccat tcagccggaa
    24421 ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg agatcgttat cggtattgtg
    24481 tgtgcgatta tggcggattt gctcttttct ccgcgatcga tcaaacaaga agtagatcga
    24541 gagctggaaa gtttgctggt cgcgcaatat caattaatgc aactctgtat caagcatggc
    24601 gatggtgaag ttgtcgataa agcctggggc gacctggtgc gacgcaccac ggcactacaa
    24661 ggcatgcgca gcaacctgaa tatggaatct tcccgctggg cgcgggccaa tcgacgttta
    24721 aaagcgatca atacgctatc gctgacgctg attacccaat cctgcgaaac ttatcttatt
    24781 cagaatacgc gcccggaatt gatcactgat actttccgcg aattttttga cacgccggta
    24841 gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc ggagagttat cgcctggacc
    24901 ggggaacggg aaacgcctgt caccatttat agctgggtcg cggcggcaac gcgttatcag
    24961 cttctcaagc gcggcgttat cagtaacaca aaaatcaacg ccaccgaaga agagatcctg
    25021 caaggcgaac cggaagtaaa agtagagtca gccgaacgtc atcatgcaat ggttaacttc
    25081 tggcgaacca cactttcctg cattctgggc acgcttttct ggctgtggac gggctggact
    25141 tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt cactggcaat gcgtttgccg
    25201 aatccacgca tggtggcgat cgactttatc tatgggacgc tggccgcgct gccgttaggg
    25261 ctgctctact ttttggtgat tatccctaat acccaacaga gcatgttgct gctgtgtatt
    25321 agcctggcag tgctgggatt cttcctcggt atagaagtac agaaacggcg actgggctcg
    25381 atgggggcac tggccagcac cataaatatt atcgtgctgg ataacccgat gactttccat
    25441 ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg gctgtgtgct cgcgttcacc
    25501 gttattttgc tggtgcggga taaatcgcgc gacaggactg gacgtgtact gcttaatcag
    25561 tttgtttctg ccgctgtttc cgcgatgact accaatgtgg cacgtcgtaa agagaaccac
    25621 ctcccggcac tttatcagca gctgtttttg ctgatgaata agttcccagg ggatttgccg
    25681 aaatttcgcc tggcgctgac gatgattatc gcgcaccagc gcctgcgtga tgcgccgatc
    25741 ccggttaacg aggatttatc ggcgtttcac cgacaaatgc gccgcacagc agaccatgtg
    25801 atatctgccc gtagcgatga taaacgtcgt cggtactttg gccagttgct ggaagaactg
    25861 gaaatctacc aggaaaagct acgcatctgg caagcgccac cgcaggtgac ggaaccggtt
    25921 catcggctgg cggggatgct ccataagtat caacatgcgt tgaccgatag ttaagtcaaa
    25981 accgacgcca aaagcgtcgg ttttttcatg gctatactta gcgatgcacg gcagaactcg
    26041 ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc tttgatttta atgagattga
    26101 gagtcaggag gatttttatc gtgactttag ccaaaccctt ggtctggcta aagataaggt
    26161 acgcgatctt gactcactat gggatgtgtt aatgaacgat gtcctgccgc taccacttga
    26221 gattgaattt gttcatctgg gagagaaaac gcgtcgccgt tttggcgcgt taatattgct
    26281 gtttgatgag gcagaggaag agctggaagg gcatttgcgt tttaatgttc gtcattagcg
    26341 caaaaaaaag cccccgaacc gggggcaata tcgtcggaca aaacgatgag ggtttatttg
    26401 tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag cttcagtaat ctggtaggcc
    26461 gttgcgcctt tctcttccgc ttttttgttc agcatttcac gtatatccat tggcgaagac
    26521 gccacaccac ttacggatac ggtcccgatt gcttcacgat tttgtgcttg tgcagcatca
    26581 atggagtcgg cagcgaatgc accgaaagag agaacagaaa gtacgcttaa tgcagctaca
    26641 gtggttttga ttttcatgat ttttacctcg acataatctt ttagctgggc ctttgtttcg
    26701 tgacccatat cacaaaatca agcatacact aattattgaa ctaattaata ccacgctaac
    26761 tgtttcagtt gatactaagt gttaaaaatg tgaattttaa taacaaaaaa gtataaaaaa
    26821 cacggttatt tattattaat tatagtattt tcaattagat aatgaatttt gcataatgtg
    26881 ctttttttac cgtatgtatt cattttgtga atgatgtgtc gcggtaaaac gcactattcg
    26941 ttaaatgtat cgcaggagaa agaaggggaa tgagagggat aagcaacatt ttccccgccg
    27001 ccagaagcga cggggcagag attaaagctc ctggtcgaac agctctaaaa tcgcttcgta
    27061 caggtctttg acggtgaaac cgttagcagg ggtggtaaag atggtgtcat cgccagcgat
    27121 ggtgcccaga ataccttctg ctttgcccag tgagtccagc aggcgagcaa ttaactgcgc
    27181 cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg tagtcgatat ccagcaccag
    27241 attcttcaat ggactggagg tggttggtac acccagttca gctggcaggc agtaaaccat
    27301 ttccattttg gcattgcgtg tacgtacagc accaaacttg gtcaacatcc gcgagacttt
    27361 agactgatta atattgtcaa agccttgctc ctgcaacgcg gcgacgattt cgccctggga
    27421 gctaaatttc tcttctttaa gtaatgcttt aaatgcttta actagttctt cttgcttagc
    27481 cgagcttcgc ataagtcacc cgatatggtg gttgatacaa cattattgtg catacagatg
    27541 aatttttatg caaacagtca gccctgaaga aggctgaaat aatgttatga aagagcggga
    27601 ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac ggcatgcaaa ttctgtttaa
    27661 aagtaaatta attgttatca aattgatgtt gttttggctg aacggtaggg tatattgtca
    27721 ccacctgttg gaatgttgcg ctaatgcata agcgactgtt aattacgtaa gttaggttcc
    27781 tgattacggc aattaaatgc ataaacgcta aacttgcgtg actacacatt cttgagatgt
    27841 ggtcattgta aacggcaatt ttgtggatta aggtcgcggc agcggagcaa catatcttag
    27901 tttatcaata taataaggag tttaggatga aagtcgcagt cctcggcgct gctggcggta
    27961 ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc ttcaggttca gaactctctc
    28021 tgtatgatat cgctccagtg actcccggtg tggccgtcga tctgagccat atccctactg
    28081 ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc ggcgctggaa ggcgcagatg
    28141 tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat ggatcgttcc gacctgttta
    28201 acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt tgcgaaaaac tgcccgaaag
    28261 cgtgcattgg tattatcact aacccggtta acaccacagt tgcaattgct gctgaagtgc
    28321 tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg cgttaccacg ctggatatca
    28381 ttcgttccaa cacctttgtt gcggaactga aaggcaaaca gccaggcgaa gttgaagtgc
    28441 cggttattgg cggtcactct ggtgttacca ttctgccgct gctgtcacag gttcctggcg
    28501 ttagttttac cgagcaggaa gtggctgatc tgaccaaacg tatccagaac gcgggtactg
    28561 aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct gtctatgggc caggcagctg
    28621 cacgttttgg tctgtctctg gttcgtgcac tgcagggcga acaaggcgtt gtcgaatgtg
    28681 cctacgttga aggcgacggt cagtacgccc gtttcttctc tcaaccgctg ctgctgggta
    28741 aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag cgcatttgaa cagaacgcgc
    28801 tggaaggtat gctggatacg ctcaagaaag atatcgccct gggcgaagag ttcgttaata
    28861 agtaattgat tagcgaataa taaaaaaccg gagcacagac tccggttttt gttttgagca
    28921 ctcgacttaa ttggttgccg gatattcctg aatggtgacc tgcagcgtta actgcttatc
    28981 atcacgcatc actacaaccg ggatcaccga accagggcga atttccgcca cctgatccat
    29041 cgtctccaga gcagagatgg ccggtttgtt atccaccgaa ataatcagat cgttgacctg
    29101 aatacccgca ttcgccgctg ggccgtcagg tgacacttca ttaaccacga tcccttgcag
    29161 ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc tcgcgtccgc cgataccaat
    29221 gtagccgcgg atcacgcgac catcgcggat cagcttatcc ataattttgg ttgctaactg
    29281 gaaaggaatc gcaaagccga taccttccgg cgtttcgcca tcgttactct tatcaaacga
    29341 cagcgtgtta atgcccatca gttctcccag cgagttcacc agcgcgccgc cagagttacc
    29401 gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc ccggtcgggt tcagaccgat
    29461 tcgacccgtg gcactaataa tcccttgggt aatggtctgc ccgaggttgt acgggttacc
    29521 gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt gcattaattg gaatggtagg
    29581 taaaccgcca gtggcattaa ttttaagtac cgccagatcg gttagagagt cagatcccac
    29641 cagcaatgct tcaaatacac gtccatcctg taaggcgacg atgatctgat cggcgtcgtt
    29701 gatgacgtgt ttattggtga tgatataacc gcgttgatcc atgattacac cggatcccag
    29761 ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa ccacggttgt aaacgttaac
    29821 caccgctggc gcggcgcggc gaaccgccag attatagctg gcaggcgtct catcggtact
    29881 gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc gaaggcatgg caaccagcag
    29941 aatagcgccg acaattaatc caatcgcaac ggaacgtaag agcttcacaa acatgatgga
    30001 ggcgtcatta aaaaagggaa cggcagcagc ataccacgag ttaaccggac atcacacgtc
    30061 agcctgatgc ccggtttacg acattaacgc atcagcagat agatgctttc attgccgcgt
    30121 acaatttgca gggcgatgat ggccggtttt gccgccagca ctttacgcat ttcagcaatc
    30181 gagttcaccc gatcgcggtt gacgccaatg atcacatcgt ctttttgcaa gccagcctga
    30241 gcagctgggc ttcctttgac aacttcatct atcttaatac ctttgccgcc atcttttagc
    30301 tgaccatcgc tcaacgttgc accttccagc gctggcgtga tcatttcagc gctggccgac
    30361 gaagaggtgt tggtatcaag cgtcacttct acttccagtg gtttgccgtt acgcagcagg
    30421 ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac gagagcgcaa ctcagcaaag
    30481 ctattcagcg gtttgccgtt gaggctggta ataatatcgc ccgctttgac gcccgctttc
    30541 gctgagccag aacttggcaa cacttcgctg acaaacgcgc cacgctgcac gtcaaggttg
    30601 aaggctttgg cgatatcggc actcatctcg gtgcctttga tgcctaacaa accgcgtttg
    30661 atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg ccatattact ggggatggca
    30721 aatccaatcc cgacgctccc gccgccaggc gcaaggattg cagtgttgat gccaattaac
    30781 tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc ggttaatgga agcatctgtc
    30841 tggataaagt tttccagacc ttcaagattc aacccgctgc ggcctaatgc ggaaacaatg
    30901 ccagaggtgg cggtttgccc aaggccaaat gggttaccga ccgctacggc aaaatcaccg
    30961 acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta atttgctcgg gttttgaatt
    31021 tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca gtttttcatc aaactcgcgc
    31081 ccatcattga gctgaatact gattttctgt gcctgattaa tcacatggtt gttggtcagc
    31141 acatagcctt tactggcgtt gatgatgaca ccggaaccta aaccttcgaa gggttgtgca
    31201 ggttgatccg gtaaatcatc accaaaaaac tttttgaatt cttccgggat tttctgtccc
    31261 tgactggccg ttccttccac ccgtacgctc accactgccg gaagcacttt ttccagcatt
    31321 ggagccagac tggggagagg ggcctgatcg gcaacctggc ctggaatcga cgcgacggcc
    31381 tgaaatgacg ccgagagagt taacccggca cttaacgcta atgcactcaa cagctgggtt
    31441 tgttttttca ttattcctgc tctcgtacct gaatgataag aaaagagatt caaaacagtt
    31501 tgatgttatt gaattttcag acggctaaca atgagacacc agattaaatg atagtcgtgg
    31561 agggagagaa gaggagggcg caatggctgc gcccgaaaaa taaattagtc gcgcttcgcg
    31621 ccagtacgca gcaggccgga tgcgccttca gaatagtcgc gaggcatctg caccggtgcc
    31681 tgatcgttgc tggcttcaga ctctgccaga cgattacgga acgggtttgc ttcagcagac
    31741 agttccggca gcaggctgct ggagcttttt gccatgtgct gatacagctg gcgatagtcg
    31801 tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt ggctaaccag ctcttcgcga
    31861 tactcgtcca gttcagcttt attcttttcc agttcgtact gcaacgcctg ttgctggcgt
    31921 agtttacgat taccaaaacg catggccaca gcaccaataa tgatgccgac gactaaccca
    31981 attagcgcat attcccaggt catgaacatc tcccgttgtc ttttgtttcc gtagggtgtt
    32041 ggcttcaggc tccatgcctg cggctgatta tgccactata accgttaatt ccacagaagt
    32101 ggaatcccga ctgcatatcg cgtagtgtag aacggccttt ttttcgtcaa ccgtgaacaa
    32161 cggcgcaccg attattcaag gaataacaat aagatcatgc aaagcgttac cccaacatcg
    32221 caatacctga aggcgctcaa tgaaggcagc catcaacccg acgacgttca aaaagaggcc
    32281 gtcagccgcc tggaaattat ttatcaagaa ctcatcaata gcaggccacc agcccccagg
    32341 acgagtgggc taatggcgcg ggtcggtaag ctgtggggta aacgcgaaga cacaaagcat
    32401 acgccagtgc gtggcttata tatgtggggc ggtgtaggac gcgggaaaac ctggctgatg
    32461 gaccttttct atcaaagcct gccgggagag cggaaacagc gcctgcactt tcaccgtttt
    32521 atgctgcggg tgcacgaaga gctaactgcc ttacaggggc agaccgatcc gctggaaatt
    32581 attgccgatc gctttaaagc cgaaactgac gtgctctgtt ttgacgaatt ttttgtttct
    32641 gatattaccg acgccatgct acttggcggt ctgatgaaag ccctgttcac ccgcggtatt
    32701 accctggtag cgacgtcaaa tattccgccg gacgaacttt atcgaaatgg cctgcaacgt
    32761 gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt gtgatgtaat gaacgtggac
    32821 gctggtgttg attatcgact gcgtacactc actcaggcgc atctgtggct ttcgccactc
    32881 aacgatgaaa cccgggcgca gatggataaa ctatggttgg cgctggcggg ggtgaaacga
    32941 gaaaattcac cgacgttaga aatcaaccat cggccattgg cgacaatggg cgtcgagaac
    33001 cagacgctgg cggtttcttt tactacgctg tgcgtcgacg cccgcagtca gcatgactat
    33061 attgcgctct cacgcctgtt tcacacagta atgttgtttg atgtgccagt catgacgcgg
    33121 ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg atgagtttta cgagcgccat
    33181 gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa tttatcaggg tgagcggctg
    33241 aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga tgcaaagcga agagtatctg
    33301 aagcgcgagc atttggcggg ttaaaacctg ccacaaatca caaaaagggg tcgatctttg
    33361 accccgactt ctctataatc ctgcgacccc acgttacaag aaagtttttt tcccaaaact
    33421 ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg ccggactttg tcgtgtgaac
    33481 ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa ctatttattg ggtaagcttt
    33541 taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg cgactggtat gttgttgacg
    33601 cgaccggtaa aactctgggc cgtctggcta ctgaactggc tcgtcgcctg cgtggtaagc
    33661 acaaagcgga atatactccg cacgtagata ccggtgatta catcatcgtt ctgaacgctg
    33721 acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt gtactatcac cacaccggcc
    33781 acatcggtgg tatcaaacaa gcgacctttg aagagatgat tgctcgccgt cctgagcgtg
    33841 tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc gctgggtcgt gctatgttcc
    33901 gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc ggcacagcaa ccgcaagttc
    33961 ttgacatcta atcgggatta taggcaatgg ctgaaaatca atactacggc actggtcgcc
    34021 gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa cggtaaaatc gtaatcaacc
    34081 aacgttctct ggaacagtac ttcggtcgtg aaactgcccg catggtagtt cgtcagccgc
    34141 tggaactggt cgacatggtt gagaaactgg acctgtacat caccgttaaa ggtggtggta
    34201 tctctggtca ggctggtgcg atccgtcacg gtatcacccg cgctctgatg gaatacgacg
    34261 agtccctgcg ttctgaactg cgtaaagctg gcttcgttac tcgtgacgct cgtcaggttg
    34321 aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc gcagttctcc aaacgttaat
    34381 tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc gcttcggcgg gtttttttat
    34441 ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt tttgctgccg tctgaaagac
    34501 aggtaaaaca aaaaccacat cgccaataag ggtctaagtc aactatttca gactaaagcg
    34561 catctctttt tccccatttc cggcatccac tcaccacaaa ggtcgcaaaa tctggtaaac
    34621 tatcatccaa ttttctgccc aaatgtcggg tattgctcat tttttgtttg attttcgaac
    34681 aaagagagtc gttccttatt gggtaacaca acttctgact ggccacctgg tggctggtag
    34741 cagtaaaaat tctgactata cctggaggtt ttcatggctg tcgctgccaa caaacgttcg
    34801 gtaatgacgc tgttttccgg tcctactgac atctatagcc atcaggtccg cattgtgctg
    34861 gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa aggacaatcc gcctcaggat
    34921 ctgattgacc ttaacccgaa tcagagcgtt ccgaccctgg tggatcgtga gctgaccctg
    34981 tgggaatctc gcatcattat ggaatatctg gatgagcgtt tcccgcatcc gccactgatg
    35041 cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca tgcatcgcat cgaaaaagac
    35101 tggtacacgc tgataaacac tatcatcaac ggttcagctt ctgaagcaga tgccgcacgt
    35161 aagcaactgc gcgaagaact gctggcgatt gcgccggtct tcggtcagaa gccgtacttc
    35221 ctgagcgatg agttcagcct ggtcgattgc taccttgctc cgctgctgtg gcgtctgccg
    35281 caactgggca tcgagttcag cggcccgggt gcgaaagagc tgaaaggcta tatgacccgc
    35341 gtctttgagc gtgactcttt ccttgcttct ttaactgaag cagaacgtga aatgcgtctg
    35401 ggccggagtt aatctgtatg gatttgtcac agctaacacc acgtcgtccc tatctgctgc
    35461 gtgcattcta tgagtggttg ctggataacc agctcacgcc gcacttggtg gtggatgtga
    35521 cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga cgggcaaatc gtactcaaca
    35581 ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga tgaggtgcgc tttaacgcgc
    35641 gctttggtgg cattccgcgt caggtttctg tgccgctggc tgccgtgctg gctatctacg
    35701 cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc tgcctacgat gaagatacca
    35761 gcatcatgaa tgatgaagag gcatcggcag acaacgaaac cgttatgtcg gttattgatg
    35821 gcgacaagcc agatcacgat gatgacactc atcctgacga tgaacctccg cagccaccac
    35881 gcggtggtcg accggcatta cgcgttgtga agtaatacaa aacaggccca ggcggcctgt
    35941 tttgtctttt taatgactta ataaaatagc gtgattgtgg caatcatatt caccacgaaa
    36001 ccgactgcca tggggatcgc tgtccgcttc accaccgcaa aaggcgaaac gcctgcaatt
    36061 cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg cgcgcgctag cgtcgtggca
    36121 aaatgcatcg gcataatcat tacaaccgct ggtacatgta gtccggctgc gatattcgga
    36181 ataagactgg caaatgacat aaacggcgca ttgccagagc ccatcacaat ggcacaaatg
    36241 gcaatgacta gcgccataat aatcatcacg ccaataccgc ccagaccaga atgctccgca
    36301 cccctgataa ccgcatcaac agtgccaatc gtcgttaagc ctttcgcaaa tatttcaccc
    36361 gcgaccacca gcgttaccac gttgacaaac tgcgtaccca tgccgtcaaa aaacgcctgc
    36421 acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga actcaacaaa catcgtcacc
    36481 gtcaaactta gtaacatcac caccaccaga tgcagttctg acttcatcag cccgacgtgg
    36541 gcgaggaaca gcgagccgag catcaggatt aacggcatca caggtaaaat ggcgtaatag
    36601 agcggcggga cattatcgag agttttttgc tctgcctgtt cgtgattgat atttttatct
    36661 tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg ccaccgagat aatgacgcaa
    36721 gaggcgaccg gaagctggta gtggaagaag tatgtggcaa ttttcattcc cgcgacctgg
    36781 gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa tggacatcgt ggttgcgata
    36841 accgccaccg cagagaggcg acttactccc agactcacca gcgtcggaaa taaggtgacc
    36901 atcagcaaca tacccagacc ggaggcgctg gtaataaact gcgccatgat ttggccgatg
    36961 acgtaagttg ccgacagaat aatatacggc gagcgaatga gttttaacgg gcggcttagc
    37021 aagctcacca tcgcgcgact ggctcctatg cgctccatgt agcgggcata accgccca
//
LOCUS       DAJTYI010000023        36807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYI010000023 DAJTYI010000000
VERSION     DAJTYI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1312
                     /gene="speC"
                     /locus_tag="OO564_001158"
     CDS             <1..1312
                     /gene="speC"
                     /locus_tag="OO564_001158"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6652362.1"
                     /translation="IYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDL
                     NENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPF
                     YPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELEDGKPWQ
                     SYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFG
                     VPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPL
                     AEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQE
                     ANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGI
                     NLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1309..2628
                     /gene="potE"
                     /locus_tag="OO564_001159"
     CDS             1309..2628
                     /gene="potE"
                     /locus_tag="OO564_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6652363.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(2684..4324)
                     /gene="pgm"
                     /locus_tag="OO564_001160"
     CDS             complement(2684..4324)
                     /gene="pgm"
                     /locus_tag="OO564_001160"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6652364.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEQHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4350..4895)
                     /gene="seqA"
                     /locus_tag="OO564_001161"
     CDS             complement(4350..4895)
                     /gene="seqA"
                     /locus_tag="OO564_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6652365.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5080..5844
                     /gene="ybfF"
                     /locus_tag="OO564_001162"
     CDS             5080..5844
                     /gene="ybfF"
                     /locus_tag="OO564_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6652366.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            5984..6277
                     /gene="ybfE"
                     /locus_tag="OO564_001163"
     CDS             5984..6277
                     /gene="ybfE"
                     /locus_tag="OO564_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6652367.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6417..6947
                     /gene="fldA"
                     /locus_tag="OO564_001164"
     CDS             6417..6947
                     /gene="fldA"
                     /locus_tag="OO564_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6652368.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7157..7243
                     /gene="uof"
                     /locus_tag="OO564_001165"
     CDS             7157..7243
                     /gene="uof"
                     /locus_tag="OO564_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6652369.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7236..7682
                     /gene="fur"
                     /locus_tag="OO564_001166"
     CDS             7236..7682
                     /gene="fur"
                     /locus_tag="OO564_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6652370.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(7766..8092)
                     /locus_tag="OO564_001167"
     CDS             complement(7766..8092)
                     /locus_tag="OO564_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652371.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8129..9535)
                     /gene="chiP"
                     /locus_tag="OO564_001168"
     CDS             complement(8129..9535)
                     /gene="chiP"
                     /locus_tag="OO564_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6652372.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10114..11778)
                     /gene="glnS"
                     /locus_tag="OO564_001169"
     CDS             complement(10114..11778)
                     /gene="glnS"
                     /locus_tag="OO564_001169"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6652373.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(11981..13927)
                     /gene="nagE"
                     /locus_tag="OO564_001170"
     CDS             complement(11981..13927)
                     /gene="nagE"
                     /locus_tag="OO564_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6652374.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14260..15060
                     /gene="nagB"
                     /locus_tag="OO564_001171"
     CDS             14260..15060
                     /gene="nagB"
                     /locus_tag="OO564_001171"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6652375.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15120..16268
                     /gene="nagA"
                     /locus_tag="OO564_001172"
     CDS             15120..16268
                     /gene="nagA"
                     /locus_tag="OO564_001172"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6652376.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16277..17497
                     /gene="nagC"
                     /locus_tag="OO564_001173"
                     /gene_synonym="nagR"
     CDS             16277..17497
                     /gene="nagC"
                     /locus_tag="OO564_001173"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6652377.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            17545..18287
                     /gene="nagD"
                     /locus_tag="OO564_001174"
                     /pseudo
     CDS             17545..18287
                     /gene="nagD"
                     /locus_tag="OO564_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            18684..20348
                     /gene="asnB"
                     /locus_tag="OO564_001175"
     CDS             18684..20348
                     /gene="asnB"
                     /locus_tag="OO564_001175"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6652378.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            20728..20804
                     /locus_tag="OO564_001176"
     tRNA            20728..20804
                     /locus_tag="OO564_001176"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20762..20764,aa:Met,seq:cat)
     gene            20813..20897
                     /locus_tag="OO564_001177"
     tRNA            20813..20897
                     /locus_tag="OO564_001177"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20847..20849,aa:Leu,seq:tag)
     gene            20921..20995
                     /locus_tag="OO564_001178"
     tRNA            20921..20995
                     /locus_tag="OO564_001178"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20953..20955,aa:Gln,seq:ttg)
     gene            21030..21104
                     /locus_tag="OO564_001179"
     tRNA            21030..21104
                     /locus_tag="OO564_001179"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21062..21064,aa:Gln,seq:ttg)
     gene            21120..21196
                     /locus_tag="OO564_001180"
     tRNA            21120..21196
                     /locus_tag="OO564_001180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21154..21156,aa:Met,seq:cat)
     gene            21245..21319
                     /locus_tag="OO564_001181"
     tRNA            21245..21319
                     /locus_tag="OO564_001181"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21277..21279,aa:Gln,seq:ctg)
     gene            21357..21431
                     /locus_tag="OO564_001182"
     tRNA            21357..21431
                     /locus_tag="OO564_001182"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21389..21391,aa:Gln,seq:ctg)
     gene            complement(21585..22760)
                     /gene="ubiF"
                     /locus_tag="OO564_001183"
     CDS             complement(21585..22760)
                     /gene="ubiF"
                     /locus_tag="OO564_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6652379.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            22906..24330
                     /gene="miaB"
                     /locus_tag="OO564_001184"
     CDS             22906..24330
                     /gene="miaB"
                     /locus_tag="OO564_001184"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6652380.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            24483..25523
                     /locus_tag="OO564_001185"
     CDS             24483..25523
                     /locus_tag="OO564_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6652381.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            25520..25987
                     /gene="ybeY"
                     /locus_tag="OO564_001186"
     CDS             25520..25987
                     /gene="ybeY"
                     /locus_tag="OO564_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6652382.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26077..26955
                     /gene="corC"
                     /locus_tag="OO564_001187"
     CDS             26077..26955
                     /gene="corC"
                     /locus_tag="OO564_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6652383.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            26980..28518
                     /gene="lnt"
                     /locus_tag="OO564_001188"
     CDS             26980..28518
                     /gene="lnt"
                     /locus_tag="OO564_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6652384.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            28915..29822
                     /gene="gltI"
                     /locus_tag="OO564_001189"
                     /pseudo
     CDS             28915..29822
                     /gene="gltI"
                     /locus_tag="OO564_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            29992..30732
                     /gene="gltJ"
                     /locus_tag="OO564_001190"
                     /pseudo
     CDS             29992..30732
                     /gene="gltJ"
                     /locus_tag="OO564_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            30732..31406
                     /gene="gltK"
                     /locus_tag="OO564_001191"
     CDS             30732..31406
                     /gene="gltK"
                     /locus_tag="OO564_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6652385.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31406..32131
                     /gene="gltL"
                     /locus_tag="OO564_001192"
     CDS             31406..32131
                     /gene="gltL"
                     /locus_tag="OO564_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6652386.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32249..33184
                     /gene="rihA"
                     /locus_tag="OO564_001193"
     CDS             32249..33184
                     /gene="rihA"
                     /locus_tag="OO564_001193"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6652387.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33268..34938
                     /gene="hscC"
                     /locus_tag="OO564_001194"
     CDS             33268..34938
                     /gene="hscC"
                     /locus_tag="OO564_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6652388.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(34998..36449)
                     /gene="djlC"
                     /locus_tag="OO564_001195"
     CDS             complement(34998..36449)
                     /gene="djlC"
                     /locus_tag="OO564_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6652389.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(36446..>36807)
                     /locus_tag="OO564_001196"
     CDS             complement(36446..>36807)
                     /locus_tag="OO564_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6652390.1"
                     /translation="WDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         8966 a         9445 c         9357 g         9039 t
ORIGIN      
        1 gatttataac gcccgccagg tggtggataa aattggtcat ctgtgtgact acattctgtt
       61 tgactccgca tgggttggct atgagcaatt tatcccgatg atggctgact gttcgccgct
      121 gttgctggat cttaatgaga acgatccggg tattctagtt acgcaatccg tacataaaca
      181 gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa gacagccaca tcaaagggca
      241 acagcgttat gtaccgcaca aacgcatgaa caacgccttt atgatgcacg cctccaccag
      301 cccgttctat ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag
      361 cggtcgtaat atgtggatgg attgtgtggt aaatggcatt aatgcccgca aactgatcct
      421 cgataactgt cagcatattc gtccgttcgt acctgaactg gaggatggta aaccctggca
      481 gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg taccagggga
      541 acactggcat tcttttgaag gctatgcaga gaatcaatac tttgtcgatc cttacaaact
      601 gttgctgaca accccaggta ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc
      661 cgcgacgatt cttgctaact tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct
      721 taactccatc ctcttcctgc tgactccggc agaagatatg gccaaacttc agcaacttgt
      781 tgccctgctg gtacgcttcg aaaaactgct tgagtccgac gcgccattag cagaagtgct
      841 gccttccatc tacaaacagc atgaagagcg ctacgccggt tataccctgc gtcagttgtg
      901 ccaggaaatg catgatttgt atgcccgcca caacgtgaaa caactgcaaa aagagatgtt
      961 ccgcaaagcg cacttcccac acgtcagcat gaatccgcaa gaagccaact acgcctattt
     1021 acgcggtgaa gtggagctgg ttcgtctgcc ggatgccgaa ggtcgtatcg ctgccgaagg
     1081 ggcgcttcct tatcctccgg gtgtgctgtg tgttgttccg ggtgaaatct ggggttgtgc
     1141 tgttttgcgt tacttcagcg ctctggaaga agggatcaac ctgctgccag gttttgcacc
     1201 ggagctgcag ggtgtctata tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt
     1261 catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg gaaaaattat gagtcaggct
     1321 aaatcgaaca aaatgggcgt cgttcagtta accatactga cgatggtcaa catgatgggc
     1381 tccggtatca tcatgctgcc gacaaagctt gccgaagttg ggacaatttc aattatctcc
     1441 tggctggtga cagccgtagg ctcaatggca ctggcatggg cattcgcaaa atgcggtatg
     1501 ttcagccgta aatcaggtgg tatgggcggt tatgccgagt atgctttcgg taaatccggt
     1561 aactttatgg cgaactatac ctacggcgtc tcactgctga ttgctaacgt cgcgattgct
     1621 atttcggcgg ttggttacgg caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt
     1681 ggtcttgcga ccatcggggt gcagtggatt tgtaccgtgg ctaactttgg tggtgcgcgc
     1741 attaccgggc aaatcagtag cattaccgtg tggggggtca ttattccggt ggtaggcctg
     1801 tgcattatcg gctggttctg gtttagcccg acgctttacg ttgattcctg gaatccgcat
     1861 catgcaccgt tcttcagtgc agtaggttct tccatcgcta tgacgctgtg ggcttttctt
     1921 ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa acccggaacg taatgtgcca
     1981 atcgcggtac tcggcggtac gttaggtgcg gcggtgattt atatcgtctc caccaacgtg
     2041 attgcaggga ttgtgcctaa tatggagctg gcaaattcaa cggcaccatt tggcctggcc
     2101 ttcgcacaga tgttcacacc agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc
     2161 tgctgcggtt cgctgcttgg ctggcagttc accattgccc aggtgtttaa atcttcatct
     2221 gatgaaggct acttccctaa aattttctcc cgtgtaacca aagtggatgc accggtgcag
     2281 ggaatgttga ccattgtgat tatccagagc gggttgccgc tgatgacgat tagcccgtcg
     2341 ctaaacagtc agttcaacgt gctggttaac ctggccgtgg tgaccaatat cattccgtat
     2401 attctgtcaa tggcggcatt agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa
     2461 gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac
     2521 tcatccgggg aagaagccat gctgtacggt tccatcgtga ccttccttgg ttggacactg
     2581 tatggtctgg tatcaccacg ctttgaactg aaaaataaac acggttaaaa aagcaaagta
     2641 aaaaagggcg atcttgcgac cgcccttttt ttattaaatg tgtttacgcg tttttcagaa
     2701 cttcgctaac aatctcaacc gcttctttct caatctgctt gcgatgttct tcaccgagga
     2761 agctttcgca gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc
     2821 cgttgtcagt catcactttc agaccgccaa tagaagcacc gttgcccgga gcagcagtca
     2881 ggcgcgcggt gatcgggtca cctgccaggg tgctggcgct caccatttcc ggagacagct
     2941 tagacagcgc cgctttttgt gcggaagtcg cagctgcctg caaacggttg tagctcggcg
     3001 caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg
     3061 tgatttccgc cgccagcaga cacataatga tgccgtcctt gtcggtggac cacggcgtgc
     3121 cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt
     3181 cgaacagacc atcaacaaac catttgaaac ctaccggtac ttctaccagc ttacggccca
     3241 agtcattgac cacacggtcg atcatcgcag aagaaaccag cgttttaccg acagcaacat
     3301 ctttgcccca ctgcggacga tgctggaaca ggtagttgat cgccaccgcc aggtagtggt
     3361 tcggattcat caaacctgcc ggagtgacga taccgtgacg gtcataatcc gggtcgttag
     3421 caaacgccag atcgaactta tcacgcagtg ccagcagacc cgccatcgca cactcggagg
     3481 agcagtccat acggatcgca ccgtctttat cgaggtgcat aaagcggaag gtttgatcga
     3541 cctgatcgtt aacgatggtc aggttgaggt tgtaatactc gccaatgcgc ttccagtatt
     3601 cgataccgga accgcccaac ggatcaacgc ccagcgtcag gcccgctttc tgaatcgccg
     3661 ccatatcaac gatatcggcc agcccttcca cgaacggctg caccagatcc tgctctttca
     3721 catagccgga tgccatcgct tcgtcgaggg agatacgctt cacgcctttc aggccatcgg
     3781 ccatcagtgc gttggccctg tcttccacca ctttggtgac gttagtatca gccgggccac
     3841 catttggcgg attgtacttg ataccaccat cttccggcgg gttatgggac ggtgtaatca
     3901 cgataccgtc tgccagcggg ccaccttttt tattgtgaac caggatggca ttggaaacgg
     3961 caggcgtcgg agtgaagcca ttgttttcct gcacaatgac atcaacgccg ttcgctgcca
     4021 gcacttccag tacggaaatg aacgcaggct cggacagggc gtgagtatct ttacccacat
     4081 agcaagggcc agtgatgccg tttttcgcac gttcttcagc aattgcctga gcgatcgcca
     4141 gaatgtgctg ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga
     4201 atttcaccgc gtgctccgca ttccctgctt ctggtttcag tacataatat tgcgccgtca
     4261 gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga ttgtggattg
     4321 ccattgcttt gtcctttgtc tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat
     4381 tccgccggga attgcatcga ctgcatgatg tgttcgatca tgctgcattt acggccggtg
     4441 ttggtgttgg tgatcaccca atacggcgtg cctggcacat gtttcggctt ggtctgatta
     4501 ccatttttca gcagcgtttg ttcatctgcc gcaaagtaca cgcgtgtacg accgtgcaac
     4561 gattccgttg cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga caacagcagc
     4621 ataaagcgat tgaccgctcg cttttgctct gcgtattcgt ccgaaagcag aagttcacgc
     4681 attgcgcgaa ccttgtcttt aatcgttttg accggcttcg cttcgacgat agcaggtgac
     4741 gcaacgcgaa cctctttcgt caccggagca gcaggctgtg atgcggcgga aaatttcaac
     4801 atacgccgta aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct ggcaatatag
     4861 ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct
     4921 gaatacaagt cgccaagagt atatgaataa atcccggcag cggatagcgt caagcctggc
     4981 ttgcagaaaa actcggaata aaccttattt gttgcagccc tgtggcacaa tggtcaacat
     5041 aataccctaa cccgacaacg cgaaaaaaag aactttgcca tgaaattaaa tatccgcgcg
     5101 caaactgcac aaaaccagca caataattct cccatcgttc ttgtccatgg tctgtttggt
     5161 agcctcgaca accttggcgt actggctcgc gatctggtaa acgatcacaa tatcatccag
     5221 gttgatatgc gtaaccacgg tctttcacca agagagccgg taatgaatta cccggcgatg
     5281 gcgcaggatc ttgttgatac cctggatgca ctgcagatcg acaaagcaac atttatcggt
     5341 cactccatgg gcggtaaagc ggtaatggca cttactgcac tggcccccga tcgcatcgat
     5401 aaactggtgg cgatcgatat cgcaccggtc gactatcacg tacgccgtca tgatgagatt
     5461 ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca agcagcagca
     5521 ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt
     5581 gacggggagt ggcgctttaa cgtaccggta ttgtgggatc agtatccgca tattgtgggt
     5641 tgggagaaaa tcccggcatg ggatcaccct gccctgttta tccctggcgg caattctccg
     5701 tatgttagcg agcagtaccg tgatgattta ctggcacaat ttccacaggc acgggcgcat
     5761 gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt attacgcgct
     5821 atccgtcgct atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt
     5881 ccgcgcgtgg gcgttggcgt gagtcatcgc gctgatgtat tatggcgcgc tatccatccg
     5941 ggcagaggcc tggctgattg tttcccccga agtcaccaag atcatggcca aagaacaaac
     6001 ggaccgtacg acattagatc tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa
     6061 tccgctttcg cgcgatgaac agctgcgtat taataaacgc aaccagctaa aacgcgacaa
     6121 agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa
     6181 cgagctggcg gaatcgcgta atatgagccg tagcgaactg atcgaagaga tgttaatgca
     6241 gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt tccactttca tgtagcacag
     6301 tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct gttctgctat gattgccttt
     6361 atccgtgggc aattttccac ccccatttca ataagtttca agaggttatt tcactcatgg
     6421 ctatcactgg catctttttc ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga
     6481 ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga cattgcaaaa agcagcaaag
     6541 aagatctgga agcttacgac attctgctgc tgggcatccc aacctggtat tacggcgaag
     6601 cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga gattgatttc aatggtaaac
     6661 tggttgcgct gtttggttgt ggtgaccagg aagattacgc cgaatatttc tgcgacgcat
     6721 tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac catcgttggt cactggccaa
     6781 ctgcgggcta tcatttcgaa gcatcaaaag gtctggcaga tgacgaccac tttgtcggtc
     6841 tggctatcga cgaagaccgt cagccggaac tgaccgctga acgtgtagaa aaatgggtta
     6901 aacagatttc tgaagagttg catctcgacg aaattcttaa tgcctgatgt gatgcggcgt
     6961 agactcatgt ctacgccgta ttaatagata atgccaatca aaataattgc tacaaatttg
     7021 taacttttgc tgttgtacct gtacaatgtc ccagtgttca agtggccttg ccgttgtaaa
     7081 tgtaagctgt gccacgtttt tattaacaat atttgccagg gacttgtggt tttcatttag
     7141 gcgtggcaat tctataatga tacgcattat ctcaagagca aattctgtca cttcttctaa
     7201 tgaagtgaac cgcttagtaa caggacagat tccgcatgac tgataacaat accgccctaa
     7261 agaaagctgg cctgaaagta acgcttcctc gtttaaaaat cctggaagtt cttcaggagc
     7321 cggacaacca tcacgtcagt gcggaagatt tatacaaacg tctgatcgat atgggtgaag
     7381 aaattggtct ggctacggta tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca
     7441 cccgccacaa ttttgaaggc ggtaaatccg tatttgagct gacacagcaa catcaccacg
     7501 atcacctgat ttgcctcgac tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag
     7561 cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct gactaaccac agtctctatc
     7621 tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat
     7681 aagccagcct gaacgagaaa agccaacctg cgggttggct tttttataca agggaaataa
     7741 gaaaagcgag agttacagct ctcacttatt tgttattact gcgtatttcc tgccagacct
     7801 tatcacaatc ggctttcacc gcctgatcat ttccggtttg cgttgcacgc agacactgct
     7861 gataatccag tacgcgtaca ctttcctggt cggcaaactg ctgatgttgt ttctctttct
     7921 tcagcacgtt taacacgctc tggcaggctt caattttttc tggcgaacct tgcgcggtgt
     7981 tgatacaggc gctgtatgcc tctttcagac ggctgtcttc ctgtggtgcg gttgattgcg
     8041 cacaagctac cagccccgat gccatcatgg cgatgagaat cagttttttc attgaatgcg
     8101 gcaggcacaa aacctgccgc gtcgggcatc agaagatggt gaatggtgcg attaccataa
     8161 atttcacgtc acgctcatcc tggaagatgt taccgtaacc gccgccccag cttgggatgt
     8221 cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt
     8281 cctgaatggt gtagaccgca tccaggctgt aggcagactc ttcaatagtc cggtttttgt
     8341 cgttgtacgc atccggattg ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt
     8401 aggaagcgcc gatggcgaag cctggaagat tccagttttt caggtcatac atcgcaccga
     8461 agaagaccgc tttttcgccg ttagcgttga agtaggaacg gttatcccac cagatatcca
     8521 ggcggccgtt ggaggaagcg taggttgggg tcatacgttg caggaagtat ccctgctgac
     8581 tgtcagcctt aacccaggtg ccttcgaggc gcaaatccac tacgtcagcc gcccggtaac
     8641 caaaggtcaa cgcttgcagc caagcggtgc cgtcataaag atcgttgacg ctgcgatcgt
     8701 caactttatc gcgggtacca tagaactggt agctggtggt taacgggcta ccggcgatat
     8761 caaatttgta gctggctttg gcaaaatatt gatcgatata cccttccgcc tgaccaaatg
     8821 ccgcttccag tacgaagtta tttttgaagt cgtatttcgc accgatggag tgcagataat
     8881 caactttggt ggttttatcg ttctgataaa actcatccat ttccagatgc cacggcgctt
     8941 tgtattcgtt ggtccacatg taggagaaac tcaaggcacc agcatcgccg taatcaaaat
     9001 tcgccccggc ttccgccccc tgataagtac ccggcataaa gctccagtgc ggcgctaaca
     9061 gcgtttgacc ggttggctga atgtaacctg ccctcgccca aaccggaccg tatttaaatt
     9121 tggccgcagc tttatacagg cttataccgc ttttgtcgcc ggaccagtct tcgtcatagg
     9181 ctttattact ttttgaaaac gcgatttcgt tcgggtggga gctgtcgccg ttttccgcca
     9241 tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat atcagcagca taaccggact
     9301 gaaaatcgag gttggcattc caggtggagt gaaaaaggtt ggttttgtat ttgtcgccgt
     9361 cggtaacatc tttacggtcg cgttcacgct gccagtaata gataccgccg gttaaggttg
     9421 aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc cataaagccc gacattgctg
     9481 taacaccggc aatagccagc gccagcgtac tacgtttgcc actaaacgta cgcatgggtt
     9541 aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac agcgaaaaaa aataaaaatt
     9601 aaaaaagtag cgttgagaaa agtctcgctg actacaggaa cagactattt ttcgcgacgc
     9661 gaattatcaa tctctttctg taaccagaat gtaagaacat gagcaaccgc acatattttg
     9721 atgatttttg ttacaaggct taagtttgcg gttgaaaggc ttgcagaaga ataaaaacgt
     9781 gaaattccag caagatgaaa gcggttacat tttatttaat tatgttttgt gattttaaat
     9841 ttattttatg gataaattgc gaagcggaat taattcgcga agaaaagcag aaaaaacgcc
     9901 gcagagcgcg gcgtttggga ttgtttgcta tttcttgccg gatgcggcgt gaacgcctta
     9961 tccggcctac aaaagcacgc aaattcaata tattgcagag atcatgtagg cctaataagc
    10021 gaagcgcatc aggcaattta gcgtttgaaa tttattgtct atttattgcc ggatgcgacg
    10081 taaatgcctc acccggcttg gtagaactta aaattactcg cctactttcg cccaggtatc
    10141 acgcagccca acggtgcggt taaataccgg tttttcggcc gtggaatggc ggctgtcgag
    10201 gcagaagtaa ccttcacgct caaactggaa tgctttaccc gctaccgcat ctttcagcga
    10261 cggttcagca aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc
    10321 atccgcagca cctgggttag gcacgctgaa cagacgatca tacaaacgga tttcaaccgg
    10381 cagcgcatgt gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg
    10441 atctttgctt aaggtgtcgg cgtcataagt acagaagatg gtggtgatat taccttcggc
    10501 atctttctcc acgcgttcag ccttaatgac gtaagcatta cgcagacgca cttctttacc
    10561 cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca
    10621 aatttcaccg ctaaacggca cctgacggct ccccatttcc ggtttgttcg gatggttcgg
    10681 catggtgacc atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat
    10741 aactgccatt gcgcgcggcg cattttcgtt gagatcttca cggatgcagg attccagcga
    10801 cgccatctca atggtgttgt cctgcttggt cacgccgatg cgtttgcaga actcacgaat
    10861 agaagccgca gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc
    10921 ccagccttca acgtgcttgt cggtcaccag caggttcagc ttacgcttgg acatcacagt
    10981 gtattccaga ttcaggcgcg agaattcgta ctggcgcgga tgaacaggaa tggtgatgtt
    11041 atccagtacc cagtcgtaca gacgacggtt gtcctggaac tcaagcgtac acagagagtg
    11101 cgtaatacct tccagcgcat cgctgatgca atgggtgaag tcgtacatcg ggtagatgca
    11161 ccacttgttg ccagtctggt ggtgttcagc aaatttaatg cggtacagca ccggatcgcg
    11221 cattacgata aatggcgacg ccatgtcgat tttcgcacgc aggcaggctt taccttcttc
    11281 aaaaccaccg gcacgcattt tttcgaacag cgccaagttc tcttcaacgc tgcggtcgcg
    11341 gtacgggctg tttttacccg gctgcgtcag ggtaccgcgg tattcgcgga tctgttccgg
    11401 tgtcagttca tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag
    11461 ctgatcaaaa taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc
    11521 tacgtcgttt ttgatcgaat cgacatactc gatatcttct tttaccgggt tagtgtcgtc
    11581 gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga
    11641 tttcgcatgg ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt
    11701 ggtgtgctta ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg
    11761 ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga
    11821 tcttataagc gggacaggct gacaactgtt agttttttaa aattgataat ctaacaagga
    11881 ttatggggat gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt
    11941 tggatgcgac gctgccgtgt cgcatcaggc ataaagcaga ttactttttg atttcataca
    12001 gcggtgtttg acccgccaca acatggccct gagctttaat gatcaagcca ctgaaatcgt
    12061 cgatattgct gcaaaccacc gggctaatca tcgagcgggc gttagcgttc aggtaatcca
    12121 gatccatttc cagaatcggt tgccctgcgc ttacctgcgc accctcttcc accagacgtt
    12181 taaagccttt accttccagc gctacggtgt cgatacccat atggacgacg atctccgcgc
    12241 ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa gattttcacg attgtccctg
    12301 cggctggtga tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt
    12361 tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac gacatcaccg gtaatcggcg
    12421 ataccagctc cgcgatagat accgcgtttg gtacagcctg cggttttgct acaggcgcgg
    12481 cagttgccgg agttgcttca gctgacgcag cggctaccgg accacgggca acgactttct
    12541 tcatcgcatc gccgatggat tctgctttcg cgccaacaat cacctgaata gtctgtttgt
    12601 tcagtttcac tacaccagaa gcacccagac gtttacacat cgtatcgttg acgcgggcag
    12661 agtcagccac ggtcaggcgc agacgggtaa tacaggcatc aatcgctttc aggttgtcag
    12721 taccgccaac cgcagcaata tagttggttg ccagttgagt cagaccttct tcagtattgc
    12781 tgttggcttc ttcagtaacg atctcgtctt ctttatcttc acgacccggc gttttcaggt
    12841 tgaacatgcg gataaccaaa ctgaacacca cgaagtagat agcgaagaag ataacgccca
    12901 tcaccagcag catccagacg ttctggctgg cggccggcag gttatacatc aacgcgtagt
    12961 cgatagcacc cgcagagaaa gagaagccgg catggatacc cagcagcgtt gccacaaaca
    13021 ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag cggcgcaagg aacatgaaca
    13081 ggaattccag cggctcagtc acaccggtca ggaacgcagt aacagcaaca gaaagcagca
    13141 tcccgccaac catcggacga cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac
    13201 ccggcagacc gaacatcatg atcgggaaga agccggacat gaacatcccc gcggtgccgt
    13261 caccagcgta gaaacggttg atgtcgccgt ggaaaaccgt acccgccgcg ttggtgaatt
    13321 caccaatctg gaaccaggcg atggtgttca gcacctgatg cagaccggtt gggatcagca
    13381 gacggttgat gaaaccaaag ataccggaac ccagcgcgcc cgcagaaacg atccactcgc
    13441 cgcctgcatg gatagcgtgc tgtaccggcg gccagacgta accaaaaatg gccgccagca
    13501 ccagacagaa gaagccggtg gcgatcggca caaagcgttt gccgccgaag aagctcagga
    13561 agtccggcag tttaatatcg gaccaacggt tataggctgc gccaccaacc agaccggtaa
    13621 tgatacccgc cagtacaccc atgttaattt ctgggttgat ggtcaccatc gctttggtta
    13681 acacaaagta acctaccgca cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc
    13741 tggatgccac accgatggcg aagattaatg cgaggttatc aaaaattgca ccgcccgcct
    13801 gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac agcgccgcca
    13861 ccggcagcac cgcgataggg agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa
    13921 tattcatctt attcccccta cgagaaccct atttggctcg tttaaagccg tatttttatt
    13981 ttgctgcaaa ttttactgcc gatgttctgt aatcagattg ttagatcatc tgctacagag
    14041 tgtgtgaaaa tttaattcgt atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc
    14101 aaatatcgtt atcatcactc ccttttactg gctaaaccag aaaacttatt ttatcattca
    14161 aaaaatcaag tcggattgac gcctgtctgc gcaaatccag gttacgctta aagatgtcta
    14221 atccgccaac ggcttacatt ttacttattg aggtgaataa tgagactgat ccccctgact
    14281 accgctgaac aggtcggcaa atgggctgct cgccatatcg tcaatcgtat caatgcgttc
    14341 aaaccaactg ccgatcgtcc gtttgtactg ggcctgccga ctggcggcac gccgatgacc
    14401 acctataaag cgttagtcga aatgcataaa gcaggccagg tcagctttaa gcacgttgtc
    14461 accttcaaca tggacgaata tgtcggtctg ccgaaagagc atccggaaag ctactacagc
    14521 tttatgcacc gtaatttctt cgatcacgtt gatattccag cagaaaacat caaccttctc
    14581 aacggcaatg ccccggatat cgatgccgag tgccgccagt atgaagaaaa aatccgttct
    14641 tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg acggtcatat tgcatttaac
    14701 gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa ccctgactca tgacactcgc
    14761 gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg tgccaaaata tgccctgact
    14821 gtcggtgttg gtacactgct ggatgccgaa gaagtgatga ttctggtgct gggtagccag
    14881 aaagcactgg cgctgcaggc cgccgttgaa ggttgcgtga accatatgtg gaccatcagc
    14941 tgtctgcaac tgcatccgaa agcgatcatg gtgtgcgatg aaccttccac catggagctg
    15001 aaagttaaga ctttaagata tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa
    15061 ttgttatccc tgccctggtt ccttgctcag ggcaatattt tttaaaatcg ggggtcagaa
    15121 tgtatgcatt aacccagggc cggatcttta ccggccacga atttcttgat gaccacgcgg
    15181 ttgttatcgc tgatggcctg attaaaagcg tctgtccggt agcggaactg ccgccagaga
    15241 tcgaacaacg ttcactgaac ggggccattc tctcccccgg ttttatcgat gtgcagttaa
    15301 acggctgcgg cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg
    15361 aaatcatgca gaaagccaat gagaaatcag gctgtactaa ctatctgccg acgcttatca
    15421 ccaccagcga tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac ctagcaaaac
    15481 atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag
    15541 gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg
    15601 aaaacgccga cgtcattacc aaagtgaccc tggcaccgga aatggttcct gcggaagtca
    15661 tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg tcactccaac gcgacgttga
    15721 aagaagcaaa agccggtttc cgcgcgggga ttacctttgc cacccatctg tacaacgcga
    15781 tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca
    15841 tttattgcgg tattatcgct gatggcctgc atgttgatta cgccaacatt cgtaacgcta
    15901 aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca
    15961 acattgaaca gttcattttt gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg
    16021 atgagaacgg tacgttaagc ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg
    16081 tcgaacattg cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc
    16141 gtgcgattga cgttgaaaaa cgtctcggca cgctcgccgc aggtaaagta gccaacctga
    16201 ccgcattcac acctgatttt aaaatcacca agaccatcgt taacggtaac gaggtcgtaa
    16261 ctcaataaga gaaagtatga caccaggcgg acaagctcag ataggtaatg ttgatctcgt
    16321 aaaacagctt aacagcgcgg cggtttatcg cctgattgac cagtacgggc caatctcgcg
    16381 gattcagatt gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca
    16441 gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag gcctccaccg ggggccgccg
    16501 cgctatctcc atcgtcaccg aaacccgcaa tttccacgca atcggcgtac ggcttggtcg
    16561 tcatgacgcc accatcactc tgtttgatct cagcagcaaa gtgctggcag aagaacatta
    16621 cccgctgccg gaacgtaccc agcagacgct ggaacatgcc ctgctgaatg ccattgctca
    16681 gtttattgat agctaccagc gcaagctgcg cgagctaatc gccatttcgg tgatcctgcc
    16741 agggcttgtt gacccggaca gcggcaaaat tcattacatg ccgcatattc aggtagaaaa
    16801 ctgggggctg gtagaagcac tgaaagaacg ttttaaagtg acctgtttcg ttggtcacga
    16861 tatccgtagt ctggcgctgg cggagcacta cttcggtgca agtcaggatt gcgaagactc
    16921 cattctggtg cgtgtccatc gcggaaccgg ggccgggatt atctctaacg ggcgcatttt
    16981 tattggccgc aacggtaacg tcggtgaaat tggccatatt caggtcgaac cgctgggtga
    17041 acgctgccac tgcggcaact ttggctgcct ggaaactatc gccgccaacg ctgccattga
    17101 acaacgggtg ttgaatctgt taaagcaggg ctaccagagc cgtgtgccac tggacgactg
    17161 caccatcaaa actatctgca aagccgcgaa caaaggcgat agcctggcct cggaagtgat
    17221 tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt gctatcaact tatttaatcc
    17281 gcaaaaaatt gttattgctg gtgaaatcac cgaagccgat aaagtgctgc tccctgctat
    17341 tgaaagctgc attaataccc aagcgctgaa ggcgtttcgt actaatctgc cggtggtacg
    17401 ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct
    17461 caacggtatt ttgctccagc atttgctgga aaattaatgt gcttttatag tggcgcttat
    17521 tgttgtcaat attctgggta gtccatgacc attaaaaatg taatttgcga tatcgacggc
    17581 gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg aatttttgca cgggattatg
    17641 gataaaggcc tgccgctggt gttgctgacc aactatcctt cgcagactgg gcaagatctg
    17701 gcgaaccgct ttgccactgc cggtgtcgat gtgccggata gcgtgtttta tacctctgcg
    17761 atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga aagcgtatgt ggtgggcgaa
    17821 ggcgcactga ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat
    17881 ttcgtgattg ttggcgaaac acgttcctac aactgggaca tgatgcataa agcagcctat
    17941 ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg acacggtttt tatcccgctt
    18001 gtggcgcgtt gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc tatgttggta
    18061 agcccagccc gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa
    18121 cagtgattgt cggcgataac ctgcgtaccg atattctggc gggcttccag gccggtctgg
    18181 agacgattct ggtgctttct ggtgtttcat cgctcgacga tatcgacagt atgccgttcc
    18241 gcccaagctg gatttacccg tcagtcgctg aaatcgacgt tatctgaata ccacccacgt
    18301 ctgcggacgt ggttggctct gatgacaaac gccaaactgc ctgatgcgct acgcttatca
    18361 ggcctacgca gctcctgcaa tatattgaat tttcatgctt ttgtaggccg tataaggcgt
    18421 tcacgccgca tccggcatga acaaatcgca ctttatcagc aatttggccc ccgtaaaata
    18481 taaccattca ataaaacccg ctaaaaatta tcgattcatc aataaataca tcctcacacc
    18541 agaccctttt tcaccaatcg acaatcacca ttacgttttt tatttttgca ctgccaaata
    18601 gcttgcgcac agtgctgctt tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa
    18661 ataccaggtt aacggagaag gttatgtgtt caatttttgg cgtattcgat atcaaaacag
    18721 acgcagttga gctgcgtaag aaagcactcg agctgtcacg cctgatgcgt catcgtggcc
    18781 cggactggtc cggtatttat gccagcgata acgccattct cgctcacgaa cgtctgtcaa
    18841 ttgttgacgt taacgcgggg gcgcaacctc tctacaacca acaaaaaacc cacgtactgg
    18901 cggtaaacgg tgaaatctac aaccaccagg cattgcgcgc tgaatatggc gatcgttatc
    18961 agttccagac agggtctgac tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg
    19021 aatttctcga cgacttgcag ggcatgtttg cctttgccct gtacgacagc gaaaaagatg
    19081 cctacctgat tggtcgcgac catctgggga tcatcccact gtatatgggg tatgacgaac
    19141 acggtcagct gtatgtggcc tcagaaatga aatcgctggt gccagtttgc cgcacgatta
    19201 aagagttccc ggcggggagc tatttgtgga gtcaggacgg cgaaatccgt tcttattatc
    19261 atcgcgactg gttcgacttt gatgcagtga aagataacgt gaccgacaaa aacgagctgc
    19321 gtcaggcact ggaagattcc gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc
    19381 tactttctgg tggtctggat tcctcaatta tttccgctat caccaagaaa tacgcagccc
    19441 gtcgcgtgga agatcaggaa cgctctgaag cctggtggcc gcagttacac tcctttgctg
    19501 taggtctgcc gggttcaccg gatcttaaag cagcccagga agtggcaaac catctgggca
    19561 cggtgcatca cgaaattcac ttcactgtac aggaaggtct ggatgccatc cgcgacgtga
    19621 tttaccacat cgaaacttat gatgtgacca ctattcgcgc ttcaacaccg atgtatttaa
    19681 tgtcgcgtaa gatcaaggcg atgggcatta aaatggtgct gtccggtgaa ggttctgatg
    19741 aagtgtttgg cggttatctt tacttccata aagcgcccaa cgctaaagaa ctgcatgaag
    19801 agacggtgcg taaactgctg gccctgcata tgtatgactg cgcgcgcgcc aacaaagcga
    19861 tgtcagcctg gggcgtggaa gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg
    19921 cgatgcgcat taacccgcag gataaaatgt gcggtaacgg caaaatggaa aaacacatcc
    19981 tgcgtgaatg ttttgagtca tacctgcccg caagcgtggc ctggcggcag aaagagcagt
    20041 tctccgatgg cgtcggttac agttggatcg acaccctgaa agaagtggcg gcgcagcagg
    20101 tttctgatca gcaactggaa actgcccgct tccgcttccc gtacaacacg ccgacctcaa
    20161 aagaagcgta tctgtaccgc gagatctttg aagaactgtt cccacttccg agtgccgctg
    20221 agtgcgtgcc gggcggtcct tccgtcgcgt gttcttcagc taaagcgatc gaatgggatg
    20281 aagcgttcaa aaaaatggac gatccgtctg gtcgtgcggt tggcgttcac cagtcggcgt
    20341 ataagtaaga aatgcggtac tgccccggag aaatccgggg ctgttttagt tattgcgctg
    20401 cttgttgatt ctgatatgca gtaaggaaat gttccgggtt tgccacatca ctgtttgaca
    20461 actcatcact gtaggccagt atttcataga ttaatttccc accactattg ttgaattccg
    20521 aatttcatcc tgtacgacaa aagaatggct gtagggtggg gaaaaaacac aaagtaaaca
    20581 ataattgacg aatatagcgc cacgctgttc gcaacctaac caaacagtca ctttcgagca
    20641 attttccttg aaaaagaggt tgacgctgca aggctctata cgcataatgc gccccgcaac
    20701 gcagataagg tatcgcgaaa aaaagatggc tacgtagctc agttggttag agcacatcac
    20761 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accatctttt ttgcgggagt
    20821 ggcgaaattg gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag
    20881 tctcgcctcc cgcaccattc accagaaagc gttgtacgga tggggtatcg ccaagcggta
    20941 aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcttc
    21001 ttcgagtaag cggttcaccg cccggttatt ggggtatcgc caagcggtaa ggcaccggtt
    21061 tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcgaag aaataatctg
    21121 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    21181 cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat
    21241 ttgttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg
    21301 aatcctcgta ccccagccaa tttattcaag acgcttacat cgtaagtgca cccagttggg
    21361 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg
    21421 taccccagcc acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc
    21481 aatgtcgaat gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt
    21541 cttatccgac ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca
    21601 tatttcagcg cctgacgttt caacacgcca gcacgctctg ccgccattaa cccgagatta
    21661 cgcacaaaac gcagcggcgg cagattatta ctgaatccgg cataaaacag atccatacca
    21721 ctttgcataa tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa
    21781 ctggcccacg cttcaccgta gctgcgggca ttcaccagaa catcaatcag ggcatcgaca
    21841 tcacgataac caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg
    21901 cccaccagcg caagccccgg ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac
    21961 gcaccagcgg caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc
    22021 gcctggagtt gcgccatatt catattctgc aactggcgaa tacgcgccgg agagtcatac
    22081 cacaccagcg atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggggta
    22141 aattgctgcc aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatgcac
    22201 gactgcgcat actgccaggc atgaacgcca attcccgcca tctgccgcac ctgagaattt
    22261 gcgccgtcgg caccaatcac cagtttcgcg cggatcgttt caccgccctt cagttccagc
    22321 tcctgaagat cattatctcg atgtaatgca atcagcgagg ttggcacacg taacgttact
    22381 ttcggatgcg cttccagcgc ctgccacaac gcctgttgca ggactgtgtt ttccaccatg
    22441 tagccaagca acggcaactt taattcggct gcgtcaaata ccacatgtgc cgtttcccac
    22501 tcccaggttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc
    22561 cctacccctt taagcaatga taccgaagcc gcgctgatcg ccgagatccg cacgtccggt
    22621 tggctgtcag cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg
    22681 tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt
    22741 tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg
    22801 atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca
    22861 ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc
    22921 catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg
    22981 ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctactg
    23041 ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc
    23101 tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca
    23161 tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg
    23221 caaacgctgc accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt
    23281 gtagatatca gcttcccgga aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa
    23341 gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgcac ctactgcgtg
    23401 gtgccttaca cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt
    23461 gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc
    23521 tggcgtggtg agaactacga cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt
    23581 gctgcgatcg acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc
    23641 gacgatatca tcgaagtgta ccgcgacacg cctgagctgg tgagcttcct gcatctgccg
    23701 gtacagagcg gttccgatcg cattctgaac ctgatgggac gtacccatac ggcgctggag
    23761 tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccgg atattcagat cagttctgac
    23821 ttcattgttg gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt
    23881 gccgacgtca atttcgacat gagctacagc tttatcttct ctgcacgtcc aggaacgcct
    23941 gccgccgata tggttgatga tgttccggaa gaagagaaga agcagcgtct gtatattctg
    24001 caagaacgta tcaatcagca agcgatggca tggagccgtc ggatgctcgg caccacccag
    24061 cgtattctgg tagaagggac ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa
    24121 aataaccgcg tggtcaactt cgagggcaca ccggatatga tcggtaaatt cgtcgatgta
    24181 gaaattaccg acgtctaccc gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa
    24241 atgggtctgc gcatggcaga aacaccggag tcggtgattg cccgcacccg caaagaaaac
    24301 gaccttggcg tgggttatta tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg
    24361 ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga
    24421 ataatttcct tataggccgg tgaatgttca gcttccggcg gcatacagat aagaggaacg
    24481 gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaatgcg cgtctgttga
    24541 gcctgtgcgg cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga
    24601 tcaatcgccg cgataaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg
    24661 cagacattct gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag attcaggata
    24721 tcgaaccgga acagatccac cttgcgatta aagaagcgcg ggtactggag caaagcgcgg
    24781 agagcgtgcc ggagtacggc aaagcggtca atatcaaaac taaacgcggc gtaattaagc
    24841 cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg
    24901 gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc
    24961 tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga
    25021 aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccactgt
    25081 acgacgcgct gtttgaaatg ctgggctttg agaaagtcga gaaactgatt gagcgcaacg
    25141 ttattgaagt cgcgccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca
    25201 ttctcgatga gagccagaac actaccatcg aacagatgaa gatgttcctg acccgtatcg
    25261 gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata
    25321 ctaaatcagg cttacgtcat gccattgaag tgctggccga tgtcgaagag atcagcttta
    25381 acttcttcca cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt
    25441 atgaagccag ggaagaagca gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc
    25501 gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga
    25561 taattccggg ttaccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca
    25621 gtttcaggaa gagtcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct
    25681 gaatctgacc taccgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt
    25741 gccgcctggc atggagatgt cgctactggg cgatctggtt atctgccgtc aggtggttga
    25801 gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca
    25861 cggcagtctg catttgttag gttacgatca cattgaagat gacgaagcag aagaaatgga
    25921 agccctcgaa acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa
    25981 agaataattt gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc
    26041 gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt
    26101 agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac
    26161 ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac
    26221 cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc
    26281 gttcgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg
    26341 gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa
    26401 gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc
    26461 gatgctgaag ccttcggcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt
    26521 aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt
    26581 attgacgaat tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactgatt
    26641 gttggtgaga ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt
    26701 cgtcatacct ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc
    26761 acccacttta gcgatgaaga agtcgacact atcggtggtc tggtgatgca ggcgtttggg
    26821 catcttccgg cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg
    26881 gccgatagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagcca
    26941 aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt
    27001 gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc
    27061 ttctctcctt acgacgtctg gcctgcggcg attatttcgc tgatggggct tcaggcgttg
    27121 acctttaacc gtcgcccact ccagtctgcc gctattggct tttgctgggg atttggcctc
    27181 tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc
    27241 ccggttaaca tcttcctagt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg
    27301 tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc
    27361 gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg
    27421 tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc accgctaatg
    27481 ggcgtggaag ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg
    27541 gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct acccttcccg
    27601 ctgcgttaca tccaatggtt taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt
    27661 cagggcgata ttccgcaatc gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag
    27721 atttactaca acgcaacggc accgctgatg ggcaaatcat cgttgattat ctggccggag
    27781 tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag
    27841 ttgcgtgata aaggtagctc gctggtaacc gggattgtcg acgcgcgtct caataagcag
    27901 aaccgctacg atacctacaa caccatcatc acgctgggta aaggtgcacc gtacagctac
    27961 gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg
    28021 gagtcgattc tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt
    28081 gggccatata tccagccgcc gctgtcggca aatggtattg agcttactgc ggctatttgc
    28141 tacgagatca ttctcggcga gcaagtgcgc gataacttcc gcccggatac cgactacctg
    28201 ctgactatct ccaacgatgc gtggtttggt aaatctattg gtccatggca acacttccag
    28261 atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc
    28321 attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc
    28381 gaggtgttaa ccactaacgt gacgccgacc accggactca caccatacgc acgtaccggc
    28441 aactggccgc tgtgggtgct gacggcactg tttggttttg ctgctgtgtt gatgagtctg
    28501 cgtcagcgac gtaaataatc ccttcttatc gtgccggaat acatcccata tttcggcacg
    28561 ctcaccggat gcggcatcag tgcattatcc ggataacaat attctcctat ccaactttgg
    28621 cacatctatt gctttgttat acaaggcaaa gcttgaacca gcatcagtac aacccggttg
    28681 caccacagca atccaccggt agcaccaaaa gagtgcattt taagaatttt gcttcagaat
    28741 ggtgcggtgc acctcgcaaa aacaaacaat aaaacatcaa tatctttaca tttagccaaa
    28801 ctaaatgtta cctaacaggc acaacactgc acaataaagt tgcagacgat aacaacacaa
    28861 acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa
    28921 ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca
    28981 gatgacgccg ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt
    29041 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
    29101 gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
    29161 aaaccggact tgcaggtaaa actgattccg attacctcac aaaccgtatt ccactgctgc
    29221 aaaacggcac tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaat
    29281 aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgttgacc aaaaagggcg
    29341 gcgatatcaa agattttgcc gacctgaaag gcaaagccgt agtcgtcact tccggcacta
    29401 cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg cgcatcatca
    29461 gcgccaaaga tcacggtgac tctttccgca ccctggaaag cggtcgtgcc gttgccttta
    29521 tgatggatga cgctctgctg gccggtgaac gtgcgaaagc gaagaaacca gacaactggg
    29581 atatcgtcgg caagccgcag tctcaggaag cctacggttg catgttgcgt aaagacgatc
    29641 cgcagttcaa aaagctgatg gatgacacta tcgctcaggt gcagacctcc ggtgaagcgg
    29701 aaaaatggtt tgataaatgg ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt
    29761 tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc gaatgacaag gcactgaact
    29821 aattacaaga accagaggcg gaaattccag ccctctcgat tgttacgtag cacggacaga
    29881 ctatacgcct gatggtcgtt ccccatcggg cctgaaaacc gcaatacgct gggtaacaat
    29941 cttcgagggt agcagttaac gctgctaccc tcttttttct ggagtagatt tatgtctata
    30001 gactggaact ggggtatttt tttacaacaa gccccgttcg gcaataccac ctatcttggt
    30061 tgggtctgga gtggttttca ggtgacgatc gctttgtcga tctgcgcctg aattatcgct
    30121 ttcctcgtcg gttcattttt cggcatttta cgtaccgttc caaaccgttt tctctccggt
    30181 ctgggtacgt tgtatgtcga actgttccgc aacgtgccgt tgattgtgca attctttacc
    30241 tggtatctgg tgatcccgga gctgctgccg gagaaaatcg gcatgtggtt taaggccgag
    30301 ctggatccca atattcagtt tttcctttca tccatgctct gcctggggct gtttaccgcc
    30361 tcccgtgttt gcgaacaggt acgcgccgct attcagtcgc taccgcgtgg gcagaagaat
    30421 gccgcgctgg cgatgggcct gacgctaccg cagacttatc gatacgtact gctgcctaat
    30481 gcttatcgcg ttatcgtccc gccgatgact tccgagatga tgaacctggt gaaaaactcc
    30541 gccatcgcct cgaccatcgg tctggtggat atggcggcgc aggcgggtaa attgctcgac
    30601 tactcggctc acgcctggga gtcgtttacc gccatcacgc ttacgtatgt gttgattaac
    30661 gcttttatca tgctggtgat gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg
    30721 gggggcaaat aatgtacgag tttgactgga gttccattgt tccttccctg ccatatctgc
    30781 tcgacgggct ggtgatcacc ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt
    30841 ggggtaccat gctggcggtg atgcgcttgt ccagcttcgc gcctgtttcg tggtttgcca
    30901 aagcctacgt taacgtattc cgctcgattc ctttagtcat ggttttgctg tggttttact
    30961 tgattgtgcc gggatttctg caaaatgtgc tgggattatc gccaaaaaat gatattcgtt
    31021 taatttcggc gatggtggcg ttttcgatgt ttgaagcggc ctactattca gagattatcc
    31081 gtgccggtat tcaaagtatt tcccgtggtc agtcgagcgc cgcgctggcg ttggggatga
    31141 ctcactggca gtcgatgaaa ctgattattc tgccgcaggc tttccgcgcg atggtgccgc
    31201 tgctgctcac tcagggcatc gtactgttcc aggatacctc gctggtgtat gtgttatctc
    31261 tggccgattt cttccgtacc gcctcaacca ttggtgagcg tgatggtacg caggtcgaga
    31321 tgatcctgtt tgccggattt gtttatttcg ttattagcct tagcgcgtcg ttgttggtca
    31381 gctacttgaa aagaaggaca gcataatgat taccctgaaa aatgtttcaa aatggtatgg
    31441 tcactttcag gtgctgaccg actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt
    31501 ttgcggcccg tctggttccg gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc
    31561 agtgcagcaa ggtgaaatca ccgtcgatgg tatcgtggtt aacgacaaga aaaccgatct
    31621 ggcaaagctg cgctcccgcg tcgggatggt attccagcat ttcgagctgt tccctcatct
    31681 gtcgattatc gaaaacctga ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc
    31741 cgctcgtgaa aaagcattga aattgctgga gcgcgtggga ctttccgcac atgccaataa
    31801 atttccggcg cagctttccg gcggtcagca gcagcgtgtg gcaatcgctc gcgcattgtg
    31861 tatggctcct attgcgatgc tgttcgacga accgacatcg gcgctggatc cggagatgat
    31921 caacgaagtg ctggacgtga tggtggaact ggcgaacgaa ggaatgacca tgatggtggt
    31981 gacccacgaa atgggctttg cccgcaaagt ggcgaatcgg gtgatcttta tggacgaggg
    32041 taaaattgtc gaagactcgc cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc
    32101 aaaagacttc ctcgcgaaaa tcctgcatta atcactctgg cgcgcatacc caattgcgcg
    32161 ccatctcacg ctttatcgtt tcgcccctcg tatctgttac atcctggcgt tacccttgtc
    32221 gcaaagaagc acacaacaag gagcaacaat ggcactgcca attctgttag attgcgaccc
    32281 aggtcatgat gacgctatcg caatagttct cgccctcgcc tcaccagagc ttgatgtcaa
    32341 agcaattacg tcttccgccg gaaaccagac accagaaaaa accttacgca atgttctgcg
    32401 tatgctgacc ttgcttaatc gcaccgatat tccggtggca ggcggcgcgg taaaaccgtt
    32461 aatgcgtgag ttgattatcg cggacaatgt gcacggcgaa agcggtctcg acggcccggc
    32521 attaccggaa ccgacgttcg caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac
    32581 gctgcgcgaa agtgcggaac ctgtcacgat tgtttctacc ggaccgcaaa ctaacgttgc
    32641 cttgctgctc aatagccatc cggaactgca tagcaaaatt gcccgtatcg tgattatggg
    32701 cggcgcaatg gggcttggta actggacgcc tgcggctgaa tttaatattt acgttgaccc
    32761 ggaagcggca gaaattgtct tccagtcagg gatcccggtg gtgatggccg gtctggatgt
    32821 tactcataaa gcgcaaatcc acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc
    32881 tgtttcaacc attgttgccg aactgctgga tttcttcctc gaatatcata aagacgaaaa
    32941 atggggcttt gtcggcgcac cgctgcatga cccatgcacc atcgcctggc tgttgaaacc
    33001 cgagttgttt accactgttg agcgctgggt tggcgtggaa acacagggga aatataccct
    33061 gggtatgacg gttgttgatt attattatct gactggcaat aaaccgaatg ccactgtaat
    33121 tgtcgatgtt gatcgtcagg gctttgttga tttactggct gatcgtctga aattttacgc
    33181 ttaacactcg tcattgatta agatcaccac gcgttacctg ggtggtctta atctatttat
    33241 ctctttttct ttcaagatat aaatgcaatg gataatgcag aactcgccat aggtatcgat
    33301 ctcggcacta ccaatagttt aattgccgtc tggaaagacg gtgccgcgca attaattcca
    33361 aataagttcg gtgaatattt aacaccatcc ataattagca tggatgaaaa taatcatatt
    33421 ttagtcggaa aaccggctgt atcacggcgt acttcgcatc cggataaaac ggcagcgtta
    33481 tttaaacgtg caatgggcag taataccaac tggcggttag gcagcgacac atttaacgcg
    33541 ccagaactgt cctctttggt attacgctca ttaaaagaag atgccgaaga atttctgcaa
    33601 cgtccgatta aagatgtggt gatctccgtt ccggcttatt tcagcgataa acaacgcaag
    33661 catacccgtt tagcagcgga cttagccggg ttaaatgcgg tacgcttaat taatgaaccc
    33721 acagcagctg cgatggcgta tggcctgcat acccaacaaa atacccgttc gctggtgttt
    33781 gatctcggtg gcggcacgtt tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa
    33841 gttcacgcct ccgctggcga caacttcctc ggtggcgaag attttaccca tatgttggtc
    33901 gatgacgttt taaagcgcgc ggatgtcaca aggaccacgc ttaacgagag tgaactggca
    33961 gccttgtacg cctgtgtgga agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc
    34021 tggcagtacc aggatgaaac gcgggaatgc gaattttacg agaacgaact ggaagatttg
    34081 tggttgccgc tgctcaatcg cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt
    34141 ctgaagccga gtcaaatcga cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg
    34201 gtgcagcgaa tcgccgtgcg tctgtttggc aaattaccgt atcaaagtta cgatccgagc
    34261 accattgtcg cgctgggcgc agcaatccag gccgcctgcc gcttacgcag tgaagatatt
    34321 gaagaagtga tcctcactga tatttgccct tactcgttgg gcgttgaggt taaccgccag
    34381 ggcgtttccg gcattttctc gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt
    34441 gtagaaactt attcaaccat gcacccggaa caggattcaa ttacggttaa cgtctatcag
    34501 ggagaaaacc acaaagttaa aaacaacatt ctggtggaat ccttcgatgt gccgttgaag
    34561 aaaaccgggg cttatcagtc gattgatatt cgctttagtt atgatatcaa cgggttgctt
    34621 gaagttgacg tgcttctgga agacggcagc gttaagtcca gagtgattaa ccacagcccg
    34681 gtaacattga gcgcgcagca gattgaagag agtcggacgc ggttatccgc attgaaaatt
    34741 tatccgcgcg atatgctcat caatcgcacc tttaaagcca aactggaaga gttgtgggcg
    34801 cgggcgctgg gtgacgagcg agaagagatt ggccgggtga tcaccgattt tgatgcggcg
    34861 ctgcagtcaa acgatatggc ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta
    34921 gccattgaga cctcataatc acggctgccg gatgcggcgt aaatgctgca tccggcatcg
    34981 gcgcaaaaaa cacatcgtca actaaacaac atcgcgattt tgccgagagc ataacctata
    35041 accagcaccg ttaaactgat tttgataatg ctggtaatgc tctgtggttt ttcaccctca
    35101 tcattttttt cttcatggtt ttcaggtgtt ggctcatcga catcttcatc ctccggctcg
    35161 agttttactt tcgtcaacag catccgggtg ataatggcct ctaatggatg agcatctccg
    35221 ccttccagcc ttgtattaag acgggccagc atctcttgtt tcggaaacgc ttcaagtact
    35281 acctgttccg ctacccgata caaatccagt aacggtgaat cttcttctgt ctcgctaccg
    35341 actctcgatt tccaccacgc ccatgctttg cgtttgaaga ataacgactc aataaacgat
    35401 tgacctaacg gcgataattt tccgctatgt aaaatttccg cccataacag ccgcgtccgg
    35461 gcgctctcac caggaacata tttcagcggt tgaccttgcg catctaccat gctccgcgct
    35521 tcgataacca tcactaataa cgcgaaataa tccgggcaat gacgcttgct ccactgcaac
    35581 aacaaattat cagcttgtgt agatgggtaa ctttcccagt acgcgccgag atcagcaagc
    35641 aggctttccc cctcgccgca gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg
    35701 ctttctggtt cttccgcttg ccactgtttg gctacaggaa tgagttccac catacctgtt
    35761 tgcaccgagc tgtaccagcg caacagtttg cgatgtaact ttgcatcatc aggaatgaac
    35821 cacggtcctt ccatcgccaa aaacgcagtg acatagtcag gatggtaacg gcagcaacgt
    35881 tccagcgcga agtaatattc aacagtctgg ttctgtaacg cgacgggata ttccagaagg
    35941 ctaaggaaat cgaaacaatc accagcctga atggcctcaa gaaaagcctc cctttcttcc
    36001 tcatccattc cttcgctgtc attaaacgaa tgccagttca aacgctctgc caacaaagaa
    36061 gcacaattta atgacagata tcgcgcttct atagcgattg cacacagcgg ccagcgtaat
    36121 tgatcgacat cctccatgtt ccaggtattt aattgctgaa taaatttctg ccaggcggag
    36181 ggctgaaaac gatcactttc tgaatccagt aatgtcctga atgcacgtag tatttcatgt
    36241 tcagcggtgg catctttttc ttcatcatct gcttcctcga caggatttac cgccagtcgc
    36301 agcgcctctt cataggcctg gcgtaatgct ttaaacccct gcggatcggt ttcgggatga
    36361 cacaacggta agcgagcaag ataagcctgg cggataatgt ctatttgcgt cgtgctttca
    36421 atttcgagga tttgccaaca tgttttcata ggtcgctcac atccttaagc gttaccgggc
    36481 attcaggctg gacagttaat ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg
    36541 ctgccagctc ttcgaacatc cgactcccgg cgctatcgta tttgtacagg gttagcgcct
    36601 cgcgtagcga agcgttgtct tcgcactgag aagattgcca gtagagacgc cctagtgaat
    36661 aggcagaaaa atagtgcatc cagctgtgat agtaatggtg agctcgtgca tagacgcgcg
    36721 attgcagcca taaactctct tcttccgtca gccagttgtt gagcacaccg gcccgtgaca
    36781 gaaaccccat tcgaacataa tcccagg
//
LOCUS       DAJTYI010000024        36383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYI010000024 DAJTYI010000000
VERSION     DAJTYI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(19..1254)
                     /locus_tag="OO564_001197"
     CDS             complement(19..1254)
                     /locus_tag="OO564_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6652391.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1401..1477)
                     /locus_tag="OO564_001198"
     tRNA            complement(1401..1477)
                     /locus_tag="OO564_001198"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1441..1443),aa:Pro,seq:tgg)
     gene            complement(1520..1606)
                     /locus_tag="OO564_001199"
     tRNA            complement(1520..1606)
                     /locus_tag="OO564_001199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Leu,seq:cag)
     gene            complement(1627..1702)
                     /locus_tag="OO564_001200"
     tRNA            complement(1627..1702)
                     /locus_tag="OO564_001200"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1667..1669),aa:His,seq:gtg)
     gene            complement(1761..1837)
                     /locus_tag="OO564_001201"
     tRNA            complement(1761..1837)
                     /locus_tag="OO564_001201"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1801..1803),aa:Arg,seq:ccg)
     gene            complement(1940..3325)
                     /locus_tag="OO564_001202"
     CDS             complement(1940..3325)
                     /locus_tag="OO564_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6652392.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3516..4256)
                     /gene="rffM"
                     /locus_tag="OO564_001203"
     CDS             complement(3516..4256)
                     /gene="rffM"
                     /locus_tag="OO564_001203"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6652393.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4259..5611)
                     /gene="wzyE"
                     /locus_tag="OO564_001204"
     CDS             complement(4259..5611)
                     /gene="wzyE"
                     /locus_tag="OO564_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6652394.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5608..6687)
                     /gene="rffT"
                     /locus_tag="OO564_001205"
                     /gene_synonym="wecF"
     CDS             complement(5608..6687)
                     /gene="rffT"
                     /locus_tag="OO564_001205"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6652395.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6684..7934)
                     /gene="wzxE"
                     /locus_tag="OO564_001206"
     CDS             complement(6684..7934)
                     /gene="wzxE"
                     /locus_tag="OO564_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6652396.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7936..9066)
                     /gene="rffA"
                     /locus_tag="OO564_001207"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7936..9066)
                     /gene="rffA"
                     /locus_tag="OO564_001207"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6652397.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVLVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9071..9745)
                     /gene="rffC"
                     /locus_tag="OO564_001208"
                     /gene_synonym="wecD"
     CDS             complement(9071..9745)
                     /gene="rffC"
                     /locus_tag="OO564_001208"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6652398.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9723..10604)
                     /gene="rfbA"
                     /locus_tag="OO564_001209"
     CDS             complement(9723..10604)
                     /gene="rfbA"
                     /locus_tag="OO564_001209"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6652399.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10623..11690)
                     /gene="rffG"
                     /locus_tag="OO564_001210"
     CDS             complement(10623..11690)
                     /gene="rffG"
                     /locus_tag="OO564_001210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6652400.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11690..12952)
                     /gene="wecC"
                     /locus_tag="OO564_001211"
     CDS             complement(11690..12952)
                     /gene="wecC"
                     /locus_tag="OO564_001211"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6652401.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12949..14079)
                     /gene="wecB"
                     /locus_tag="OO564_001212"
     CDS             complement(12949..14079)
                     /gene="wecB"
                     /locus_tag="OO564_001212"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6652402.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14135..15181)
                     /gene="wzzE"
                     /locus_tag="OO564_001213"
     CDS             complement(14135..15181)
                     /gene="wzzE"
                     /locus_tag="OO564_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6652403.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15193..16296)
                     /gene="wecA"
                     /locus_tag="OO564_001214"
     CDS             complement(15193..16296)
                     /gene="wecA"
                     /locus_tag="OO564_001214"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6652404.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16536..17795)
                     /gene="rho"
                     /locus_tag="OO564_001215"
     CDS             complement(16536..17795)
                     /gene="rho"
                     /locus_tag="OO564_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6652405.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17880..17981)
                     /gene="rhoL"
                     /locus_tag="OO564_001216"
     CDS             complement(17880..17981)
                     /gene="rhoL"
                     /locus_tag="OO564_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6652406.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18122..18451)
                     /gene="trxA"
                     /locus_tag="OO564_001217"
     CDS             complement(18122..18451)
                     /gene="trxA"
                     /locus_tag="OO564_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6652407.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18582..19847
                     /gene="rhlB"
                     /locus_tag="OO564_001218"
     CDS             18582..19847
                     /gene="rhlB"
                     /locus_tag="OO564_001218"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6652408.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19981..21465
                     /gene="gppA"
                     /locus_tag="OO564_001219"
     CDS             19981..21465
                     /gene="gppA"
                     /locus_tag="OO564_001219"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6652409.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21512..23533)
                     /gene="rep"
                     /locus_tag="OO564_001220"
     CDS             complement(21512..23533)
                     /gene="rep"
                     /locus_tag="OO564_001220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6652410.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23620..23901
                     /gene="ppiC"
                     /locus_tag="OO564_001221"
     CDS             23620..23901
                     /gene="ppiC"
                     /locus_tag="OO564_001221"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6652411.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23947..25422)
                     /gene="ilvC"
                     /locus_tag="OO564_001222"
     CDS             complement(23947..25422)
                     /gene="ilvC"
                     /locus_tag="OO564_001222"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6652412.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25572..26462
                     /gene="ilvY"
                     /locus_tag="OO564_001223"
     CDS             25572..26462
                     /gene="ilvY"
                     /locus_tag="OO564_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6652413.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26459..28003)
                     /gene="ilvA"
                     /locus_tag="OO564_001224"
     CDS             complement(26459..28003)
                     /gene="ilvA"
                     /locus_tag="OO564_001224"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6652414.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28006..29856)
                     /gene="ilvD"
                     /locus_tag="OO564_001225"
     CDS             complement(28006..29856)
                     /gene="ilvD"
                     /locus_tag="OO564_001225"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6652415.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29921..30850)
                     /gene="ilvE"
                     /locus_tag="OO564_001226"
     CDS             complement(29921..30850)
                     /gene="ilvE"
                     /locus_tag="OO564_001226"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6652416.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30870..31133)
                     /gene="ilvM"
                     /locus_tag="OO564_001227"
     CDS             complement(30870..31133)
                     /gene="ilvM"
                     /locus_tag="OO564_001227"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6652417.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31130..32776)
                     /locus_tag="OO564_001228"
     CDS             complement(31130..32776)
                     /locus_tag="OO564_001228"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6652418.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32779..32829)
                     /locus_tag="OO564_001229"
     CDS             complement(32779..32829)
                     /locus_tag="OO564_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652419.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32915..33013)
                     /gene="ilvL"
                     /locus_tag="OO564_001230"
     CDS             complement(32915..33013)
                     /gene="ilvL"
                     /locus_tag="OO564_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6652420.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33365..34885
                     /locus_tag="OO564_001231"
     CDS             33365..34885
                     /locus_tag="OO564_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR6652421.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34910..35248)
                     /gene="maoP"
                     /locus_tag="OO564_001232"
     CDS             complement(34910..35248)
                     /gene="maoP"
                     /locus_tag="OO564_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6652422.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35367..36206
                     /gene="hdfR"
                     /locus_tag="OO564_001233"
     CDS             35367..36206
                     /gene="hdfR"
                     /locus_tag="OO564_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6652423.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36302..36377)
                     /locus_tag="OO564_001234"
     tRNA            complement(36302..36377)
                     /locus_tag="OO564_001234"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36342..36344),aa:Trp,seq:cca)
BASE COUNT         8883 a         9952 c         9166 g         8382 t
ORIGIN      
        1 ttccaaatgg ccgctttttt acttattcac caccatcaaa tgtgcctgca taatgtcact
       61 ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg
      121 gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag
      181 catgaagcgg tgtttcgggc agcctcccca acatgctttt aacacgttac aactgcgaca
      241 ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat
      301 catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg
      361 atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg
      421 atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat
      481 cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtca aaaatcgcca ccagaaattc
      541 accaaattca tcggggcgca cagaccattc cgtcagttcc ccctgaatat caccaggcgc
      601 atgtagtttc agtccatcgc ggacagctgt ttcatcagcc aggcgctcga ccaccggaat
      661 aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgta acggttgctg
      721 cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcaggag
      781 cgtcagggca cgcatcacca gtttatgcgt gggtctgccg cctttggtca cgcgatattg
      841 attgtggatc tcagccggac catccagcga taacccaaca agaaaattat tttctgccag
      901 aaatgcacac cattcgtcat cgagcagcac gccgttagtc tggaaactgt tacttatctt
      961 cctgccagca ccatatttcg cctgtagcgc cacggcacgg cggtaaaact ccagcccgag
     1021 tagcgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcagtt cgctggcggc
     1081 gatatagtga cggatatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc
     1141 gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga
     1201 accgctcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcgc
     1261 tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag
     1321 ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc
     1381 gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc
     1441 ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta
     1501 gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
     1561 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc
     1621 taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct
     1681 ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac taccaccgca
     1741 gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc
     1801 cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat
     1861 aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgctc gtttggttac
     1921 ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc tttaccgtgg cgattaagcc
     1981 caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccgaca aacagcgaca
     2041 tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta
     2101 tgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat
     2161 gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa
     2221 gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca
     2281 ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaacc ggtacgccgt
     2341 gacgggaaac ttttgccatt gccgccggta actgacggtt tttcgccagt gcgtagagca
     2401 tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa
     2461 agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc
     2521 tgccgttgct gccaatttca ttccacggga agatggtgac gataacgaaa atcgcgccta
     2581 cgtagaaaat caggatccgc cacagcactt tgcctacggc actgcgcagc gtcacctgcg
     2641 gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca
     2701 caatacacag agcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg
     2761 tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca
     2821 ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga
     2881 tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc accgcgatca
     2941 atgcgggtat ccactgcgcc atctccgggg accaaaactg gacataaacg ccaatggcgg
     3001 tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg gtgagatagc
     3061 caaaaaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta accggttcga
     3121 ggaacaacat ttcgcccatt gaacgcatga tgaaaaaaac gaacagcccg gcgatgatat
     3181 aggccaacaa tacggatggc ccggcccatt tcagggtact ggcggccccc ataaacaggc
     3241 cgacgccaat ggtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct
     3301 gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt
     3361 taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat
     3421 ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacga
     3481 agtgaccact ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag
     3541 cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg
     3601 tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg
     3661 aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag
     3721 tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg
     3781 ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga
     3841 ctgccaacga tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact
     3901 tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc
     3961 tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt
     4021 actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc
     4081 ctgacctcgg cgttatcttc aatggtcagc attttttcgg cattaatggc aaccagcgtt
     4141 ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg catgctgcat atcacgccaa
     4201 ccaatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt
     4261 atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt
     4321 caaaaagcca gtacaacagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa
     4381 agactacgcg tgagacaaac gaatccagcc cttcacgtgc cagcacgatc atattgaaga
     4441 tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattagtc tcgcggttgc
     4501 ccagctcata cagccagtcg aaccatttga tgatcagtcc aaccacaatc gccccgagtg
     4561 ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca
     4621 gtccggagtg gttattcagt acttcccagg taaagtaatt ggctgagttc agcaccatac
     4681 tcgggcgccc cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag
     4741 ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccacg
     4801 gtgaaaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat
     4861 tcattccata gcgttttaac gccagccaga acatgccaac aatccccagc acgcccgccg
     4921 ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga
     4981 atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg
     5041 cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga aagtagacca
     5101 ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac
     5161 tggaaaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga
     5221 agatgccgac gcttaccagc gcgataccca tcaggatcac ccacgtaaga ttggtctcca
     5281 cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg
     5341 ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca
     5401 agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga
     5461 agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca
     5521 cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacca
     5581 caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc
     5641 ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt tatccgccga
     5701 cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag
     5761 cactggcaaa tgctgttccg tcatatcctg ccagaacgga ttttccctgt taagcacaca
     5821 aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat
     5881 aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc
     5941 gctcaaaatt tgcagatttt cttcgctgaa taactccaac cccgcctgac gaacttcctc
     6001 aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc
     6061 aaattgctga tgaacggcac gtaaagcggc gacatgctca ttgctgcggt cgccggagtt
     6121 ccccaccaga atggtcattt ttccttcacg ttgccgatcg ttcgccatcg tattgagaga
     6181 agggtccatc cgcgtcggga agtacagcag ttctccccgc acctttgggt gcgttttggc
     6241 aaaaaagctc aaatcgccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg
     6301 acgtagtggg taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc
     6361 ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag
     6421 tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc
     6481 gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca
     6541 gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcacgcgt gctcgctcgt
     6601 cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga
     6661 tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa
     6721 caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca
     6781 tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct gacttccgcc
     6841 agaatataaa accgcagtga cgctttggcg atcaccagat aaccaaagac ataagcgccc
     6901 acttttaaca catcacccac taactgccag gcaaagagat cgcgcataac ggtaaattta
     6961 ttcgacaaca gcaaccagat agcaaaatca cgcagcaacc agacggtaaa actcgctgcc
     7021 gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt
     7081 tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc
     7141 aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac
     7201 tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc
     7261 aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc
     7321 agatagctta acgggatgac accacgttta atcaacatga tggcggcagg aattaccacc
     7381 agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgccaaa acggtaactg
     7441 acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg
     7501 gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc
     7561 accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacaa tccctggctg
     7621 attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc
     7681 gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg
     7741 tatttggtta caccgttaaa gatgccagcc ccggcaagca cgccgagcac ggtaatcaac
     7801 tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacaccgc cagcaactta
     7861 ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct
     7921 tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt
     7981 gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt
     8041 gtagcgatct tcaccgtgga actcaccaaa gtgttccccc gcagggcaac cgtgcagcgg
     8101 aatgtaatga aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg
     8161 gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg
     8221 aatcgacggc aactcgatac gcccggcttt cgccagaggc gctaacgtat cgtagtagtt
     8281 ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag
     8341 gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt
     8401 atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc
     8461 tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtgta
     8521 gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc
     8581 tttgtaagtg gacatcacgc cctgagcggc atcttccacc acaaacaaat tatgcttttt
     8641 cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccagcacgat
     8701 aacgcgcgtt ttgtcggtga tcgctgcttc aatcaacgtt tcgtcgatgt tcatggtgtc
     8761 cgggcgaaca tccacaaaga cgattttcgc gccacgcagc acaaaggcat tggcggtgga
     8821 gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc
     8881 cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg
     8941 ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct
     9001 acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg
     9061 aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta
     9121 tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac
     9181 cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctgca ccgcgtccag
     9241 ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca tagccgcgaa
     9301 tatcgccgga cgccgcacgt agaatcagac attgatgatc aaaggtgccg cgcacggcat
     9361 tttcaatcca ctgtgcataa aagcgaccgc tggcgtcagg cgcataccac ggcgcacgaa
     9421 aacggctttg cgcaaatgcg gcgctagcta actgacgtaa tgcgggaata tcggtctctt
     9481 gtgccacgac agcaccgcta tcactgacat tgttcacggg tagcgccaaa tcaacttcac
     9541 cttctaccag ggagaatccc agctgttgca gggcatccag ttcccccgta tttgatgccg
     9601 caattttggc ctgcacccgt gaccatggcg ctaaggcgtc tgacgtcagg agcggtgctt
     9661 cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg
     9721 gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt
     9781 ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag
     9841 gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaaa
     9901 gtgctggcct caatcaggct gtcgtgagtg ccggtatcca gccaggcaaa tccgcgcccg
     9961 agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc
    10021 agttcaccgc gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag
    10081 aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttcaagcgag
    10141 atagcgcgga aattgtcgtc aaattccacc acgccaaagc gttccgggtc catcacctga
    10201 tagtcaaaaa ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag
    10261 ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc attaaggaag
    10321 gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcatattcc
    10381 agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc
    10441 ggcgtagtga tgatgagaat ttcgcggata ccggccagca tcagcaccga cagcggatag
    10501 taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga
    10561 tgcaatcggg taccggaacc gcccgccagg ataataccct tcatgtttgc ctccgctgaa
    10621 aattagcctt tcagacctaa acgctcaccc tgatagctgc cgtcctgcac ctgcttccac
    10681 cagctttcat tagccagata ccactgaacc gttttacgca ttccactttc aaaggtttcc
    10741 tgcggcagcc agccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca
    10801 tgccccggac ggtcagcgac aaaggtgatc aggtcacgat agtgcgccac gccgtgcggc
    10861 ttgttcgaag ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta
    10921 cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa
    10981 tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca
    11041 tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc
    11101 ggaaagtggt aaggaccgta gttattcgag cagttggtga tcagcgtcgg cagaccgtag
    11161 gtccgcagcc aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga ataagggctg
    11221 ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat
    11281 acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta
    11341 ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata
    11401 aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca
    11461 cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact
    11521 ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg
    11581 taggtcagct tatcgaccac taccaccgca tcgctcgttt cgttgatgat ataacgcacc
    11641 agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga
    11701 ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata actttgaact
    11761 gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg
    11821 ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca
    11881 gcgtttcgcc gctgtgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc
    11941 gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg
    12001 cgcgtttatc ggtagcagca aggcagtcgg caaccgccgc tttcacctga tcgataaccc
    12061 agaacggttt gtgatcgtta acttcgcgcg cggtacggat aagccgcgcc tgctgggggt
    12121 tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag
    12181 gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa
    12241 tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga
    12301 agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac
    12361 cttcgaggaa aattttgtac aattcgctgg cgcgggccga acaaaccggc gtcataccac
    12421 caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaat acacgttccg
    12481 ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtcagatccg
    12541 ggcgcatctc tgctaaccat tcagccatct tctcggttga ccccaccggc gaggtggatt
    12601 caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa
    12661 cgtaggtcat atctggctca tgatcgcctt taaacggcgt aggtacagca atcagccagg
    12721 catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta
    12781 ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg
    12841 cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg
    12901 taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat
    12961 ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt
    13021 atgggcgcgg ctcatagctt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc
    13081 gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg
    13141 acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg
    13201 aatgccgcct gagtcagtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa
    13261 atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg
    13321 ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt
    13381 ggcgatgtct gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac tctcacgcct
    13441 atgaccggtc accagaatca tctttttatc gggggcgata aacgggtaat ttaccgccag
    13501 ttctgaacgc agcgtgtcgc tgctcatcac ctggtcacgc acccataaca gtgcatcaat
    13561 gactgtatta ccggtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg
    13621 ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt
    13681 agcctcttcc ggccacggcg aatagagatc gcccgtgcgc agaccagcct caacgtgacc
    13741 aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgtcg tcgtatcgcc
    13801 gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat
    13861 ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg
    13921 tacaatggaa aagagtttca gtacctgatc aagcatctct cgatgctgcg cagtgacgca
    13981 aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggag ccatcttgat
    14041 ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc
    14101 ggcgagcgcc tttgcgctca ccgcagcagt gttgctattt cgagcaacgg cgggttaatg
    14161 cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac
    14221 gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac
    14281 gcggatccag ggttggacca acattcaggg tgtttaacat ggctcgattc tgatcatagt
    14341 cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac
    14401 gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc
    14461 gcgaaatatt atgctgctca gcaattttca gcgcctgctc aatgctgttc atccggcggt
    14521 cgtagatggc tttcgccacc tcttcctgac gcttcacctg agctttcatc tggatggtgc
    14581 gtgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa
    14641 aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcggca ataagcttca
    14701 cgctgtcatt gaccgcgcga gtaaagtctc cggggataaa ctggatgttg ttaatcattt
    14761 catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg taatagtcgg
    14821 tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat
    14881 aggcttcgtc catgaccgat ggttgatcgg cagaagccat gtttgaacgg acatccaggt
    14941 tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg
    15001 taatcgccgt cgagctccac tcctgacgag caaaaaaagt atacgccagc gcgattaacg
    15061 caaacgccag ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc
    15121 cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca
    15181 tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg
    15241 ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag
    15301 gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc
    15361 tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg
    15421 acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa
    15481 tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat
    15541 ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc
    15601 gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac
    15661 tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat
    15721 catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat
    15781 tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt
    15841 gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag
    15901 caccatctcc caggagccaa agatataacc caggctactg agataaagtt tgccgaacac
    15961 catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa
    16021 acgatcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc
    16081 atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat
    16141 ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa
    16201 accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat
    16261 actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta
    16321 ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac
    16381 cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacatgcc agcaaattcc
    16441 aggaattttc ctaacatcgc cagcgcgaca ttaagattac agatataaaa gcaaaacgcc
    16501 acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag
    16561 aaatcgtcat tggtcttggt catggccagt ttattaatga ggaattccat tgcatcgatt
    16621 tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagtc
    16681 gtgagcagct cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt
    16741 ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg
    16801 taaataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatggtcagg
    16861 ctgccgccct cttccacgtt acgcgccgca ccaaagaagc gtttcggacg atgaagggca
    16921 ttggcgtcca caccaccggt caacacttta cctgacgccg gaacaacagt gttgtaagcg
    16981 cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg
    17041 cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca
    17101 aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga
    17161 cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctga
    17221 gcaatgttct gcagcagcat agttttaccg gctttcggcg gtgccacaat cagaccacgc
    17281 tgaccacgac cgataggtga tgccagatcc agtacgcgag cagttaaatc ttcagtagaa
    17341 ccgttaccac gttccatacg cagacgagag tttgcgtgca gcggggttaa gttctcaaag
    17401 aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc
    17461 gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg
    17521 cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg
    17581 taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca
    17641 tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc
    17701 tgcttacgca tgcgagccag gttttccagc cccatatttt cgccgagagt gatcagctca
    17761 gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg
    17821 gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt
    17881 catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa
    17941 gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc
    18001 gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt
    18061 tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc
    18121 cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt gcgcccactt
    18181 tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat
    18241 atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct
    18301 gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact
    18361 ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt
    18421 cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt
    18481 gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata
    18541 cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa
    18601 cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa
    18661 aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc
    18721 gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa
    18781 cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccgcgtg
    18841 ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccacgccgac gcggaaccgc
    18901 tggcggaagc taccggcctg aagctgggcc tggcttacgg tggtgatggc tatgacaaac
    18961 agctgaaagt gctggaaagc ggcgttgaca ttctgattgg caccacgggt cgtttaattg
    19021 actacgccaa gcagaaccac attaacctcg gtgccattca ggtagtggta ctggacgaag
    19081 ccgatcgcat gtacgatcta ggctttatta aagatatccg ctggctgttc cgccgtatgc
    19141 cgcctgcaaa tcagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg
    19201 aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa
    19261 cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc
    19321 tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaac
    19381 accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga
    19441 caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc
    19501 tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc
    19561 acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta
    19621 caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag tatgcattga
    19681 atttgcctgc tattgagacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg
    19741 acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccgcgc acaggcaatg
    19801 gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata
    19861 ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aatcccgatg agcttactgt
    19921 agtaagtgat tcgggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt
    19981 atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg
    20041 ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaattaa acgcaaagtg
    20101 cgtctggctg ctggcctgaa tagcgaaaat gacctctcta atgaagcaat ggagcgcggt
    20161 tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc
    20221 gttgtcgcta cggcgacatt acgacttgcc gtcaatgcgg gtgattttat tgccaaagcg
    20281 caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc gcgtctgatt
    20341 tatcagggcg ttgctcacac cactggtggt gccgatcagc gcctggtggt ggatataggc
    20401 ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg
    20461 tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaagaa
    20521 aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta
    20581 cggtatcacg gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa
    20641 atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaattgaaa
    20701 cagcgagcca ttcattgcgg tcggctggag gaactggaga ttgacgggct gacgctggaa
    20761 cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat
    20821 attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg
    20881 catctggccg tcgagcagga tattcgcagc cgtacactgc gtaatattca acgccgcttt
    20941 atgatcgata ttgatcaggc acagcgcgta gccaaagttg cggctaactt cttcgatcag
    21001 gtggaaaacg aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgtcag
    21061 cttcatgaaa tcggcctgag cgttgacttc aaacaagctc cgcaacacgc tgcttatctg
    21121 gtgcgtaacc tggatcttcc cggttttacc cccgcacaga agaaactgct ggcgacgcta
    21181 ctgctcaacc agactaaccc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtacca
    21241 ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ctatcatttt tgccagccgt
    21301 cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg
    21361 acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa
    21421 aaccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga
    21481 tgcgatgctg acgcatcttc tccggccttg attatttccc tcgttttacc gccatcatcg
    21541 ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggca ctgaccactt
    21601 tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct
    21661 ccgggcgcac cagttcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct
    21721 tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat
    21781 cgatactgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa
    21841 actccagccc ttttgacgca tggagagtca tcagttgcac ctgatccagc tcttcttcac
    21901 tctcaccacg ctccatcatg tcgcgtaaag taaagcgcgt caccacctgg gtgagcgtca
    21961 tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga
    22021 cgttcttcat gcgcatttcg gcagctttcg ggctgggcga tgtttcgtac agccaggatt
    22081 cataatccat gccgtggatc agatcgcgca ccgcggcaat cggctcccgc tccgccagac
    22141 gctggatttc tgccaaccag tgagtgaagc gggtcaatgc ttcataacca cgtccgctaa
    22201 gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg
    22261 cccactcacc cagctttttc agcgtcgccg ggccaatctc tcgcttcggc gtgttaacga
    22321 tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt
    22381 ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt
    22441 tttgcatcag gaatttttca aacacccgcg actgatggtt accgcgataa agaatagcgt
    22501 aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct
    22561 cggcttcatg ttcttcgtta ttcgcgctta aaacttttag ctctgtgccg taacccagtt
    22621 cggagaacag acgcttttca aagacgtgcg ggttattggc gatcaggatg ttcgccgctt
    22681 tcaaaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc agcgccggaa
    22741 aatcctggct cagcagcacc aggttttgcg gacgtgcgcc gcgccaggag tagatcgact
    22801 ggtcatcgtc acccaccacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt
    22861 catactggct ggtgttggtg tcctgatatt cgtccaccag caggtagcga attttgttct
    22921 gccagcgctc gcggacttct tcattgcgtt gcagaagtag cgtcggcaat aaaatcagat
    22981 catcgaagtc gagcacatta caggctttca ggtgtgcatc atacagccca taacaatggg
    23041 caaaaatacg gtcccgctcg ccaatcgcac tggctgccgc ctgggacggt gttttgagat
    23101 cgttcttcca gttagagatg gtcgaaatca actgttgcag gagaactttg tcatcttcaa
    23161 tcagcccttc ggtcaactct ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa
    23221 agttcgcctt catccccagc gccgcatact cacgcttgat gatatccagc cccagcgtgt
    23281 ggaaggttga gatcatcagc ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct
    23341 ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat
    23401 aaccgcaccc gcggatcaga tgggcgattt tattggtgat aacacgagtt ttaccggaac
    23461 ccgcacccgc cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg
    23521 ggtttagacg cataggaaaa tcgctcaatg aaagtcggga ggggaaaata acagcgtggt
    23581 agtatagcca gcctcatcct taccactcaa ggcacgatca tggcaaaaac agcagcagca
    23641 ctgcatatcc ttgtaaaaga agagaaactg gctctggatc ttctcgtgca gattaagaac
    23701 ggtgccgatt tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc
    23761 ggtgatttag gtgaattccg ccagggtcag atggttccgg cgttcgataa agttgtgttc
    23821 tcttgtccgg tactggagcc aaccggcccg ctgcacaccc agttcggtta ccacatcatt
    23881 aaggtgctgt accgtaacta atagcaaggc cttctccggg agaaggcctt gaactctgaa
    23941 tcgctattaa cccgcaacag caatacgttt catatccgtc atatagccgc gcagtttctt
    24001 acctacttgc tcaatcgcat ggctgcgaat cgcttcattt acatcacgca gctgcgcgtt
    24061 atctaccgca ccttccggaa tagctttacc caaatcgccc ggttgcagtt ctgccataaa
    24121 cggtttcagc agcggcacac aagcgtaaga gaacagatag ttaccgtact cggcagtatc
    24181 agagataacc acgttcattt catacagacg cttacgggcg atggtgttgg caatcagcgg
    24241 cagttcgtgc agtgattcat agtaagcaga ttcttcgatg atgccggaat caaccatggt
    24301 ttcgaacgcc agttcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa
    24361 gtactcctgc tcgccgattt tgccttcata ctgcggcgcg gtttcgaagg cggttttgcc
    24421 ggtctcttca cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc
    24481 ggaggagaat tctccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat
    24541 ctctttcagc tgttcagaca acgcgtaagc acgcagtttc gccgggttag agagacggtc
    24601 catcatcagt gtgataccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg
    24661 aattagtttt tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag
    24721 cagcgagcca gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt
    24781 cacttccgca acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc
    24841 ccatgcttta gcgatcgcca tgccttcgcc tttcggatcg ttttccgggt gaacggcaat
    24901 cagcgtcggt acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt
    24961 cggcgcaacc atgactacgg tgatgtcttt acggatctgc tcgcccactt cgacgatgtt
    25021 gaaaccgtgc gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac
    25081 tacatcagag tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gtgggatcag
    25141 ttcttcgtaa gtacccactt taaaaccatt ttcggtcgct ttacgccagg acgcgcgctt
    25201 ctcggcaatc gcttctttac gcagagcgta ggagatatcg agaccagaat cacgcatgtt
    25261 cagaccctgg ttcagacctt gtgcgccaca gccgacgatg actacttttt taccctgaag
    25321 gtagctcgcg ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc
    25381 cagctgctga cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtggtgt
    25441 tgtgcttctt attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt
    25501 tattgcggaa attgatatat tcacaacgtc acattgcaat ttctgcaacg tcaacattga
    25561 gggctgcgcc attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc
    25621 attttggccg cagcgcgcgg gcgatgcacg ttagcccgtc cacgctctca cggcagattc
    25681 agcgcctgga agaagatctc ggccagccgc tgtttgtgcg cgataaccgc acggtgacgc
    25741 tgactgaagc gggcgaagaa ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc
    25801 agctgcgcca caccatcgac cagcaagggc cgttgctcta tggcgaatta catatcttct
    25861 gctcggtgac cgccgcttac agccatctgc cgccgattct ggatcgcttc cgcgcggagc
    25921 acccgtcggt ggagatcaaa ctcactaccg gtgatgcggc agatgcaatg gaaaaagtcg
    25981 tcacaggtga agctgatctg gctattgccg ggaagccaga gactctgccc ggcgcggtgg
    26041 cgttttcgat gctggagaat ctggcagtgg tgttgattgc acccgcattg ccctgcccgg
    26101 tgcgtaatca ggtttctgtc gaaaagccgg actggtcaac ggtaccgttt atcatggccg
    26161 atcaggggcc ggtgcgccgc cgcattgagt tgtggttccg acgcaataaa atcagtaacc
    26221 cgatgattta cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct
    26281 gtggcgtggc attgttgccg gaagtggtgc tggaaaacag ccccgaaccg gtgcgtaacc
    26341 gcgtgatgat tttagagcgc agcgatgaga aaacgccgtt tgagctgggc gtgtgtgcgc
    26401 agaaaaagcg gctacatgag ccgctaattg aggcattctg gaagattttg ccgaaccatt
    26461 aacccgccaa aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca
    26521 gctcattcag ccgggtttcg aaatccggtt catggtcgcc aagttcgaac gccgccagta
    26581 cgcgcccgta gtcggtgcca tggctgcgat agtggaacaa agaaatgttc cagtgcgtac
    26641 ccagcgtgtt gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg aagctgtaga
    26701 ggcgttcctg caacggatgt gacggacgcc cgccgaccat atagcgcaca tgcaacttcg
    26761 ccatttcgtc gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc atctgcaaaa
    26821 tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg caggcgtttt
    26881 tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc agttggcaga
    26941 atttgaggaa gctgcctttt tcttccggaa tggtcaccgc caacaacgct tcacgctgtt
    27001 cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg
    27061 aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc
    27121 ccgccagcgc cagcgcgcca gagggttccg ccaccgcacg cacatcttcg aataaatcct
    27181 tcatcgccgc acagatcgca tcgctatcga cggtgatgat gtcgtcgaga tactcctggc
    27241 ataaacggaa ggtttcgtca ccgatgcgtt ttaccgcgac accttcggcg aacagtccca
    27301 cgcgtggcag atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt
    27361 ccgcttctac ggcgatcact ttgatttgcg gcatcagttg tttgatcagc accgctacgc
    27421 ccgccgccag accgccgccg ccgactggca caaatacgcg gtcgagatgg gagtcctgct
    27481 ggagcagttc cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg
    27541 gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa
    27601 agttcgcgcc atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt
    27661 cggcggtggc ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg
    27721 cgacgccctg cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt
    27781 ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc
    27841 gatcttcgcg cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt
    27901 gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt
    27961 cggcaccttc cggagcaccg gacaggggtt gcgagtcagc cattattaac cccccagttt
    28021 cgatttatcg cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcgcag
    28081 ggcaaaggaa acctgacgtt cacggttttt cggcgtccag gctttgtcac cccgagcttc
    28141 ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt
    28201 cgggatgtcg atagcgatca ggtcaccatc ttcaatcaga ccaatgctgc cgccgcttgc
    28261 cgcttccggt gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc
    28321 ggtgatcagc gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag
    28381 catttcctgc atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc
    28441 cgcgacaact ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt
    28501 cgccgggccg gtgaatttga ggatgctgtc atcgacgccc gcggttttca cgatgcagcc
    28561 gttttcagca aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc
    28621 cagcgagcgg atacagccgt ttgcgcgatc gtcatcgaga gaatcccaac ggcaatcctg
    28681 cgagaatgcc tgtgtagtac gaatgcccgc agggcctgcg cggaacatat tttttaccgc
    28741 gtcatcctgg gtcagcataa cgtcgtattg ttccagagtt tgcggcaacg tcaggccaag
    28801 tacgtttttc acatcacggt tcagtaaccc cgcgcgatcc agttcaccga gaataccgat
    28861 aacaccacca gcacggtgaa catcttccat atggtatttt tgggtgctcg gcgcaacttt
    28921 gcataactgc gggactttac gcgaaagttt gtcgatatca ctcatggtga agtcgatttc
    28981 cgcttcctgc gccgccgcca gcaggtgaag taccgtgttg gtagagccgc ccatcgcgat
    29041 atccagcgtc atagcatttt caaacgccgc tttgctagca atattgcgcg gcagtgcact
    29101 ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca atgcgtttac cggcattgag
    29161 gaacaactgc ttacggtccg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag
    29221 gcccagcgct tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggagcc
    29281 gcaggtcgga cacgcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc
    29341 tgcgccctgg atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt
    29401 tttcccggcc tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga
    29461 agccatcagc atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc
    29521 gcagtgggcg ttgaccatat actcaacgga atcagcgatc agttcgcgag atggaagtga
    29581 ataaagcatc cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc
    29641 tttggcaacg ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag
    29701 atggacgtga cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttgcc
    29761 aaaatcggcg tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg
    29821 accatgagtg gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt
    29881 ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta
    29941 accagcccca tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc
    30001 gtttggtaac cgggccacaa cggccttcgc caatctgaat accgtctacg ctgcgcactg
    30061 gcgtgatttc tgccgccgta ccggacataa agacttcatc cgccaagtac agggattcgc
    30121 gcgacagcac ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat
    30181 cacgggtaat acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt
    30241 tcacttcaaa caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga
    30301 taccttcctg ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt
    30361 taccaccggc ttttgccgca gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa
    30421 ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag
    30481 cggcgataat cacatcggtt gagtatcccg ctggcgggtt tactcccatg ccaacatcac
    30541 cgacgaagat cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac
    30601 gacaagcttc catcagctca tcaatgctct gtgaaaccgg gaagcgatag attttggcgg
    30661 agtcatgcag acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgtgagt
    30721 cgtagcaacg gatgccttca aaaaccgagg tgccatagtg cagcgcgtgc gacatcacat
    30781 gcaccttcgc gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct
    30841 tcgtggtcat ttttatattc cttttgcgtt caggcgcgga tttgttgtga tgtggttgtg
    30901 ctctggcaga tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag
    30961 tcgaccgacc gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg
    31021 gcagccatat tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt
    31081 tctaaggttt ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt
    31141 tctccaacat ttctgaatta ctggcacctg gcggcaccag cggccagacg ttctcaagtt
    31201 cgtcgattga gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg
    31261 cttcaacctg gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca
    31321 tgaggaaatc ggggttatcg gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt
    31381 gctgccattg tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta
    31441 actgcttgcg ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg
    31501 agatacagac aacggtatcg ttcggtcgcg cgacttgtgc gccaactgcc gccggtaaac
    31561 caaaacccat ggtacctaag ccgctggagg tgatgaaatt ttccgggcga gtgtgggcga
    31621 tgtgctgcgc agcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag
    31681 gtttacgatc cgacagttgt tttaacaaca acggagcgta gatagcgtca ccgggatggt
    31741 cgtaacgcca ggaatgttca tcacgcagct gcgcgcagtg ttgctgccag tcattgatat
    31801 ttaacggctg ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg
    31861 cctgacgcag cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg
    31921 gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca
    31981 gcaggtcaca ctcctgcacc gcgaagtttg ccgctttggt tccgtgcatc cccagcatgc
    32041 ccagatagta cggataatct gcttctactg cgcccagccc tttcagcgta caggtggcag
    32101 gcatttttgt ggcagcgaga aattcacgca aagccggaac tgcctgcgcc atccccacgc
    32161 caccgccaac gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcga
    32221 cttcggcatg tgggaaagtc acttcgtttt caacggtggt gaaccacggt tccagatcgc
    32281 cgctggctaa ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc
    32341 tggcaacgtc gaatgcttca gccatgatgc gcggcaactc ttccagagac tgcaccagaa
    32401 agctgtgctt ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtcgg
    32461 tgccgataaa cggtgcggac acctgaccag tgatggcaac gacagggatg gaatctaaca
    32521 gtgcgtccgc aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata
    32581 cgccagtttt accggtagca cgggcataac cgatagccgc tattgccgca ccctgctcat
    32641 gtcggcacag caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg
    32701 cgccacccgg ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc
    32761 actgtgcgcc attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct
    32821 gttattcatt gtctgctcct cggttatgtt tttaaggtca aaaaaacccc cggacctttc
    32881 ggtgcggggg tcttagttcg ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag
    32941 ccccgcacgg tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta
    33001 gaagggctgt cattttgtct tttcttgcat cttgttcgaa ggaatgccta aagagttacc
    33061 atagattttg cgaatagcac aagatatttt tgggcctatt tttatgatgg ctttaataat
    33121 ttcaatagaa attgttgttt tttaaaaaga aaatagtgag tgaaaaaatt caatttcagt
    33181 ataaatgttg ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca
    33241 ttccaccgcc agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa
    33301 ttcacgaatt agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg
    33361 gtttatgtca ctgtcaattg ttcatacccg cgcagccctg gaagtaaatg cgcccccaat
    33421 cactgttgag gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaacaga
    33481 aacaacggtg aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga
    33541 atatccggcg aaaaaaatca ccatcaacct tgctccagcc gatctgccaa aagaaggggg
    33601 acgatatgat ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa
    33661 taagttagat gaatatgaat tagtcggaga actagcgctt acaggcgctc tgcgtggcgt
    33721 tcccggtgca atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc
    33781 gaaagataac gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca
    33841 tctgcaggct gtctgtgcgt ttctggaagg taagaacact ctcgaacgcc cgaaaccaac
    33901 tgatgcagta tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg
    33961 aaaacgagga ctggaaatta ccgccgctgg tgggcacaac cttttactga ttgggccgcc
    34021 gggaacaggt aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa
    34081 tgaagaggca ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa
    34141 acaatggcgg cagcgcccgt tccgctcacc tcatcacagt gcgtcgttaa ctgcgatggt
    34201 gggcggtggt gcaattccag ggccaggtga aatttcgttg gcgcataacg gcgtgctttt
    34261 tcttgatgag ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga
    34321 atccgggcag atccatcttt cacgcacacg agcaaaaata acctatccag cccgtttcca
    34381 gcttgttgcg gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg
    34441 cacgccagaa cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt
    34501 cgatctctca ctggagatcc cattaccccc ccccggcatt ttgagtaaaa cggtagtgcc
    34561 gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa
    34621 gcggcagaat aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact
    34681 tgagagcgaa gatgcgatgt ggttggaaga aacgctgatc catctggggt tatcgattcg
    34741 tgcctggcag cggttactga aagttgcacg aaccattgct gatattgatc agtctgacat
    34801 tatcacacgt cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat
    34861 ccatctgcaa aaactactga cataaaaaaa gggcatttcg ccctttttat taatcgtcag
    34921 aatcggtgta gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac
    34981 gttttggacg cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg
    35041 gctcacgttc gccacggcat actgctacga agagtttctc ttcctcggta accggctcgc
    35101 gtttgccaag atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt
    35161 tgatggtgaa atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc
    35221 gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag
    35281 ttatggcgcg gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa
    35341 cgacaaataa ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag
    35401 ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag
    35461 tgagctttcg aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca
    35521 gaaacaatat ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca
    35581 tgagcacctg gcaggccgcc cgcaaggagg tggcgcatac ctcacgacat aacgagtttt
    35641 ctatcggtgc cagcgcctcg ttgtgggaat gtatgcttaa tcagtggttg ggacgcttgt
    35701 atcaaaatca ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt
    35761 ctctggtaaa acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca
    35821 aaatggacga atttagtagt cagttgctgg ggtatttcac tttagcgctt tataccagtg
    35881 ccccttctaa actaaaggga gatcttaact atctgcgact ggagtggggg ccagattttc
    35941 aacagcatga ggcaggtttg atcggtgctg acgaagttcc cattctgaca accagttctg
    36001 ctgaactggc acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct
    36061 gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc
    36121 tttatgccat atggctgcaa aatagcgata aaaatgcgtt gattcgcgat cttttgaaaa
    36181 ttaacgtgct ggatgaagtg tattaatctg aatggctggc aaggatgccg gtagaaggat
    36241 ttacttcgga gaggattatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
    36301 ctggcagggg cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc
    36361 aattgaacta cgcccctaat tag
//
LOCUS       DAJTYI010000025        34876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYI010000025 DAJTYI010000000
VERSION     DAJTYI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..746)
                     /locus_tag="OO564_001235"
     CDS             complement(<1..746)
                     /locus_tag="OO564_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652424.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNRLYIINMPLFIINELHL"
     gene            1114..2244
                     /gene="fruB"
                     /locus_tag="OO564_001236"
     CDS             1114..2244
                     /gene="fruB"
                     /locus_tag="OO564_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6652425.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2244..3182
                     /gene="fruK"
                     /locus_tag="OO564_001237"
     CDS             2244..3182
                     /gene="fruK"
                     /locus_tag="OO564_001237"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6652426.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3199..4890
                     /gene="fruA"
                     /locus_tag="OO564_001238"
     CDS             3199..4890
                     /gene="fruA"
                     /locus_tag="OO564_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6652427.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5314..6255
                     /locus_tag="OO564_001239"
     CDS             5314..6255
                     /locus_tag="OO564_001239"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6652428.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6243..7181
                     /gene="psuG"
                     /locus_tag="OO564_001240"
     CDS             6243..7181
                     /gene="psuG"
                     /locus_tag="OO564_001240"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6652429.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7275..8525
                     /locus_tag="OO564_001241"
     CDS             7275..8525
                     /locus_tag="OO564_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6652430.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8596..9294)
                     /gene="yeiL"
                     /locus_tag="OO564_001242"
     CDS             complement(8596..9294)
                     /gene="yeiL"
                     /locus_tag="OO564_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6652431.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9425..10366
                     /gene="rihB"
                     /locus_tag="OO564_001243"
     CDS             9425..10366
                     /gene="rihB"
                     /locus_tag="OO564_001243"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6652432.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10466..11716
                     /locus_tag="OO564_001244"
     CDS             10466..11716
                     /locus_tag="OO564_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6652433.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11772..12860)
                     /locus_tag="OO564_001245"
     CDS             complement(11772..12860)
                     /locus_tag="OO564_001245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6652434.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(12863..13720)
                     /gene="nfo"
                     /locus_tag="OO564_001246"
     CDS             complement(12863..13720)
                     /gene="nfo"
                     /locus_tag="OO564_001246"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6652435.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13794..14843)
                     /locus_tag="OO564_001247"
     CDS             complement(13794..14843)
                     /locus_tag="OO564_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6652436.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14942..15823
                     /locus_tag="OO564_001248"
     CDS             14942..15823
                     /locus_tag="OO564_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6652437.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16029..17498
                     /gene="lysP"
                     /locus_tag="OO564_001249"
     CDS             16029..17498
                     /gene="lysP"
                     /locus_tag="OO564_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6652438.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17790..19781
                     /gene="cirA"
                     /locus_tag="OO564_001250"
                     /pseudo
     CDS             17790..19781
                     /gene="cirA"
                     /locus_tag="OO564_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(19813..20649)
                     /gene="yeiG"
                     /locus_tag="OO564_001251"
     CDS             complement(19813..20649)
                     /gene="yeiG"
                     /locus_tag="OO564_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6652439.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            20907..21575
                     /gene="folE"
                     /locus_tag="OO564_001252"
     CDS             20907..21575
                     /gene="folE"
                     /locus_tag="OO564_001252"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6652440.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21592..22749
                     /locus_tag="OO564_001253"
     CDS             21592..22749
                     /locus_tag="OO564_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6652441.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            22891..23930
                     /gene="galS"
                     /locus_tag="OO564_001254"
                     /pseudo
     CDS             22891..23930
                     /gene="galS"
                     /locus_tag="OO564_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24210..25208
                     /gene="mglB"
                     /locus_tag="OO564_001255"
     CDS             24210..25208
                     /gene="mglB"
                     /locus_tag="OO564_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6652442.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25269..26789
                     /gene="mglA"
                     /locus_tag="OO564_001256"
     CDS             25269..26789
                     /gene="mglA"
                     /locus_tag="OO564_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6652443.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            26805..27815
                     /gene="mglC"
                     /locus_tag="OO564_001257"
     CDS             26805..27815
                     /gene="mglC"
                     /locus_tag="OO564_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6652444.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(27886..29121)
                     /gene="preA"
                     /locus_tag="OO564_001258"
     CDS             complement(27886..29121)
                     /gene="preA"
                     /locus_tag="OO564_001258"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6652445.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29115..30355)
                     /gene="preT"
                     /locus_tag="OO564_001259"
                     /pseudo
     CDS             complement(29115..30355)
                     /gene="preT"
                     /locus_tag="OO564_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30549..30788)
                     /locus_tag="OO564_001260"
     CDS             complement(30549..30788)
                     /locus_tag="OO564_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6652446.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(30791..31510)
                     /gene="sanA"
                     /locus_tag="OO564_001261"
     CDS             complement(30791..31510)
                     /gene="sanA"
                     /locus_tag="OO564_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6652447.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31660..32544)
                     /gene="cdd"
                     /locus_tag="OO564_001262"
     CDS             complement(31660..32544)
                     /gene="cdd"
                     /locus_tag="OO564_001262"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6652448.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32674..33369)
                     /locus_tag="OO564_001263"
     CDS             complement(32674..33369)
                     /locus_tag="OO564_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6652449.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33366..33764)
                     /locus_tag="OO564_001264"
     CDS             complement(33366..33764)
                     /locus_tag="OO564_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6652450.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34003..>34876
                     /gene="dusC"
                     /locus_tag="OO564_001265"
     CDS             34003..>34876
                     /gene="dusC"
                     /locus_tag="OO564_001265"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6652451.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELF"
BASE COUNT         8550 a         8500 c         9217 g         8609 t
ORIGIN      
        1 agatgcagtt cgttgataat aaatagcggc atgttgatga tgtacagtct gttcgagccc
       61 cacatcaatg tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct
      121 tcaaccgtgc cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc
      181 atcacgccgc aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa
      241 cccatcgcta acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg
      301 cgtaaaaaag agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca
      361 agggcaaaca tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat
      421 agcaaaacaa agtagttgcg attccaggca aaaagggtgc aggccagcac gcctaacagg
      481 cagcaaaaga caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac
      541 tgacttacca gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga
      601 cgggcatgta tttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca
      661 ataccggtga gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg
      721 ctggagactg cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt
      781 ttacgctgct tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa
      841 atgccatttc actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg
      901 gattttccca tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg
      961 ctcataactt tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg
     1021 tttcagcgcg atcttgtctt taaccctaag ccaggttggc gctttttttc tcatagaggc
     1081 tgaatcgttt caattcagca agagaggaga actatgttcc agttatccgt acaggacatc
     1141 catccgggcg aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg
     1201 gtgcaggccg gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa
     1261 acctcaacgt tcctcggcaa ttgtattgct attccacacg gcactaccga cacccgcgat
     1321 caggtgctga aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac
     1381 ggtcaggtag cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg
     1441 ctacgccagc tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca
     1501 acaacagcag aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag
     1561 ctcgacaacg aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg
     1621 ctgaacgctg cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc
     1681 atcaatgaac aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc
     1741 aatctgcgta gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa
     1801 acggcagcca tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag
     1861 cgtctcgctg atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca
     1921 acgttgctgg cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag
     1981 tttgtggtgc gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc
     2041 attaaacaat ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca
     2101 aacggacgta gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc
     2161 tttaccgccc agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct
     2221 gctggtcttg gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg
     2281 cttatgacct tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa
     2341 ccaccggtct gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg
     2401 gaattgatgt caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac
     2461 tgttcagcga gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta
     2521 acgttaagct gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggttttgaag
     2581 tcacccccgc cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg
     2641 atatggtctg tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact
     2701 ggatgactcg cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt
     2761 tagtagcagg tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa
     2821 tctgggcagg ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac
     2881 gtgaacaagg tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta
     2941 atgcctccgg cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg
     3001 caggggattc tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac
     3061 acacactgcg tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta
     3121 ttaccgatcg tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact
     3181 gacagcagga gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca
     3241 cgcgcctata tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc
     3301 atcgacaatc cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac
     3361 agcgcgctga acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct
     3421 gagctgttcc tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg
     3481 acagcaccgg ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact
     3541 ggcgtagcac acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc
     3601 tggtgggtga aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag
     3661 gaagtagccg cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa
     3721 tttgctggta aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag
     3781 gaactggata aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg
     3841 gcgaccactg aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc
     3901 tcttacatgc tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt
     3961 ggtatcgaag cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt
     4021 ggttcagcct ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc
     4081 gatcgtccgg gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct
     4141 ggcttcattg gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt
     4201 acgcaactga aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta
     4261 atttccagtc tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc
     4321 attctcgaag ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg
     4381 ggggcgatcc tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg
     4441 tacgcattcg gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg
     4501 gcggcaggta tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa
     4561 ttcgacaaag cgcagcagga aggtggcaaa gccgctctgg tattggggct gtgcttcatt
     4621 tcggaaggtg caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc
     4681 gtgggtgggg cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg
     4741 cacggtggtc tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg
     4801 gtagcaatta ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg
     4861 gaagtggacg cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc
     4921 tgccctgtaa catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga
     4981 atttaaatgg ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt
     5041 tcctcaaggg cagattattt tctgcccttt tttagtgttc ctgttctgtg tatcccgtca
     5101 caaattcctc gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct
     5161 gcccattttg cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca
     5221 tgtttaaata tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga
     5281 cagggttggt aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt
     5341 ataggttcgg cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgtggat
     5401 tcaaatccag gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac
     5461 ctggcgttgc tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt
     5521 caatcgctgc taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg
     5581 ccgggagaaa atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt
     5641 gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt
     5701 gaatttattc agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg
     5761 gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgtcg
     5821 aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt
     5881 gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct
     5941 gcctggttcc atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt
     6001 tattacagcg atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt
     6061 aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga
     6121 atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt
     6181 gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca
     6241 gaatgtctga attaaaaatt tcccctgaat tattacaaat ttctccggaa gtgcaggacg
     6301 ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcaac cattatttct cacgggatgc
     6361 cgttcccaca aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg
     6421 cagtacctgc cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa
     6481 aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac
     6541 cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg
     6601 cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg
     6661 cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg
     6721 ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa
     6781 ccttcggtgt gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta
     6841 ccagctcgtt tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg
     6901 cggtgaaatg gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac
     6961 agtttgcgat gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg
     7021 aagagcaggg cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc
     7081 tgaccggcgg tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg
     7141 cgagtgaaat tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc
     7201 cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg
     7261 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa
     7321 tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac
     7381 tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag
     7441 tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct
     7501 tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta
     7561 tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
     7621 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc
     7681 tcaacatcag caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg
     7741 agatcccggc gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg
     7801 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
     7861 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt
     7921 ttgcacgtat tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt
     7981 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
     8041 tgaaaatcgc cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
     8101 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
     8161 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
     8221 ccaaccttgc tggtagcctg attgggcaga aactggcgat taacgaattc atcgcttacc
     8281 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta
     8341 tatcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
     8401 tttcggctat ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
     8461 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
     8521 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
     8581 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
     8641 cgccagtcct gacaactgct ttctgttttt aattagatat cctttcttgc ttttaattaa
     8701 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
     8761 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcttgtg agagtaatat
     8821 aaatgctgcc aggcgattca ttaacggaaa tgattgattc tgagttaaag aaacaatatt
     8881 acgataattt ttatgactta aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa
     8941 taacagcgga cggtaatgtt tcatagggag cgcaaggcac caacactctt caatagcctg
     9001 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcgccaataa aacagggagc
     9061 agcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
     9121 gcctcgcgtc agataaaaca gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg
     9181 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa
     9241 cgcagattca ctcatcaatt gctgcttaag gtcattattg tggatttctt tcacgatgtt
     9301 tatccgctat gagatttctc atatttagca tacgtacgct gttgttaaga ttcaatcatc
     9361 agcccggtcg aattttcttt gctgtagaaa aaaataacgc aactggaaac agaggaaata
     9421 aacaatggaa aagagaaaaa ttattctgga ttgtgagccg ggtcatgatg atgctattgc
     9481 aatgatgatg gcagcgaaac atccggcaat agatttatta ggcatcacta ttgtagcggg
     9541 taatcagacg cttgataaaa cattaattaa tggcctgaat gtttgccaga aactggagat
     9601 taatgttccg gtttatgcgg ggatgccgca acccattatg cgtaaacaaa tcgttgccga
     9661 taatattcac ggtgaaaccg gactggatgg cccggtattc gagccgctga cccgccaggc
     9721 agaaagtacc catgcggtga aatatatcat cgataccctg atggcaagcg atggcgatat
     9781 cactctggtg ccggttggtc cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc
     9841 aatcctgccc aaaatccgtg aaattgtgct aatgggcggc gcttacggta caggcaactt
     9901 caccccatct gccgagttca atatctttgc cgacccggaa gccgcccgcg tggtgttcac
     9961 ctccggcgtt ccattagtga tgatgggcct cgatcttacc aaccagaccg tttgcacccc
    10021 ggatgtaatt gctcggatgg aaagggtagg cggccctgcc ggagagctgt tcagcgacat
    10081 catgaacttc actctcaaaa cgcagttcga atactacggc ctggctggcg gcccggtgca
    10141 tgacgccacc tgcattggtt atctgatcaa ccctgatggc attaaaaccc aggatatgta
    10201 cgtcgaagtg gacgtcaaca gcggcccttg ctacgggcgt acggtctgcg acgaactggg
    10261 cgttcttggc aagcccgcta ataccaaagt cggcatcact attgataccg actggttctg
    10321 gggcttagtc gaagagtgcg tgcgcggcta catcaaaacc cattaagtgt tgataacaag
    10381 ccgggacagt atcgcaagat gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc
    10441 aaaatgacat gaagagataa tcactatgga tgtcatgaga agtgttctgg gaatggtggt
    10501 actgctggca attgcgtttt tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt
    10561 tggcgcggcg ttagtgttac aggtcgtgat tggcgggatt atgctttggt taccgccagg
    10621 gcgttgggtc gcagaaaaag tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc
    10681 aggtagcgca tttatcttcg gttctctggt cggaccgaaa atggatacct tatttgatgg
    10741 cgcaggattt atctttggtt tcagggtatt accggcaatt atcttcgtca ctgcactggt
    10801 gagtattctc tactacatcg gtgtgatggg gatcttaatt cgcattctcg gcggtatctt
    10861 ccagaaagct ttaaatatca gcaagatcga gtcattcgtc gcggtcacca ccattttcct
    10921 cgtgctaaac gaaattccgg cgatcgtcaa accctttatc gatcgtctga atcgcaatga
    10981 attatttaca gcgatttgta gtggcatggc ctcgattgct ggttcgacaa tgattggtta
    11041 cgccgcactg ggcgtacctg tggaatattt gctggcggca tcgttaatgg cgatccctgg
    11101 cgggatcttg tttgcccgcc tgttaagccc ggctacggaa tcttcgcagg tttcttttaa
    11161 taacctctct ttcaccgaaa caccgccaaa aagcattatt gaagccgatg cgacaggggc
    11221 aatgaccggg ctgaaaatcg ccgccggtgt agcgacagtt gttatggcat ttgtcgccat
    11281 cattgcgtta attaacggta ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc
    11341 ctcgctggag tccattttag gttacctgct ggccccactt gcgtgggtaa tgggtgttga
    11401 ctggagtgat gcaaatcttg ccgggagttt gattggacag aaactggcaa taaatgaatt
    11461 tgtcgcttat ctcaatttct caccctatct gcaaacggct ggcactctgg atgctaaaac
    11521 cgtggcgatt atttccttcg cgttgtgcgg tttcgctaac tttggttcta tcggggtggt
    11581 ggtgggggcg ttttctgcgg ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt
    11641 acgcgcgctg gcggcggcga cactttctaa tctgatgagt gccaccattg ccgggttctt
    11701 tattggttta gcgtagtgtg caatttatga gccggattag atgcggtcaa gcgtcgaatc
    11761 cggcaacatt gttacaccat tggcactaat gacagcgcgt tatcggcaga cagggtaggg
    11821 ttgtttaagc tgccgctggc gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg
    11881 tcacggaaat tgtttccctc cagaaagcta tataccagac ccgccataaa accatcgtca
    11941 gcaccaaaac tgtctaccgt cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc
    12001 ttttcgctgc aataaactga ctcatcgggc aaataaacaa acagttgctg aacaccttgc
    12061 tgatgcaatg cattcactgc ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat
    12121 aaaatttcca gctccggtaa agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg
    12181 attttgcccg ctttgaattc tgaaacggta tcgacaaaca ccgggatctc atcagcgagg
    12241 gtaaagaccc attccagcgc ctcggctgtc aggttgcaat caaccagtac cacgcccgca
    12301 tgacgaagta aatcgcgcga cccgttcagt agttgcggtg tcaactgttc cagcagatgg
    12361 gtatcgttaa tcgccagcac ggtttgatcg tctcgattgg caattgccag atacgtcgat
    12421 gtgctttgac catgcaaacg aacgcagccg gagacattca cgccggcacg gcgcgtttct
    12481 tccaggagca tttcgccata aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg
    12541 cctaacagcg ccagattgtg ggcgatattg cgtccaacgc cgcctgccga acaatgaatt
    12601 gtaccgggat gagaagccgc ttgcgggtaa tggatatccg ccatcccgcg aatatccata
    12661 ttgattgtcc ccaccactac gcagtattcc tgctcggtga gaatgtaacc tttgccttta
    12721 atccggcctt tgcgcattaa atccataata tgcgccgcaa cgcgcgaacg actgatttgc
    12781 agcatgtccg caatttcgtt ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc
    12841 tccttttccc ggttattcat cttcaggcta ccgctttttc agtttgttgc gctttcagcc
    12901 aggcgatctc ttctgcccag atatccgggt tgatggtttc aaggatcagc ggaatgccgt
    12961 cgaaacggtc gttctgcata atccagcgga acgcatcatg accgatattg ccttcaccga
    13021 ggctatgatg gcggtcaacg cggctgccaa aggtactttt cgcatcgtta aggtgcatcc
    13081 cgcgcagata cttaaagccg acaatacggg caaaatcagc gaacgttttt tcgcattcgg
    13141 ctggagtacg caaatcatac ccggcagcga aagcatggca ggtatcaatg cagacgccga
    13201 cgcgggattt atcttccacg ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta
    13261 agttactgcc ctgaccggcg gtgttttcaa tcaccgctgt tacaccttga gttttatcca
    13321 gcgcaatgtt gatggattcg gcaatacgcg caaggcaatc ctcttctgaa atctgcatca
    13381 gatggctgcc agggtggaag ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt
    13441 catctataaa ggcatcgcgc gatttttcca gagcttcagt gaccggatgt ccgaggttga
    13501 tcagataact gtcgtgggga agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg
    13561 ctttgaattc atcgatggtt tgcgtcgtga gcggtgcggc acgccactga cgttggtttt
    13621 tggtgaacaa ggcaaacgcg gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca
    13681 gaccgccagc agcactaacg tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc
    13741 cgctggagga aaacggtaat gatagcgggt taacaggagc aagaagtagt ggtttatgcg
    13801 atgacgcttt gaatcacata gttaatcgca ccaccaccaa caatcagcca ggcaaacagt
    13861 accagtgcca tcagcagagg tttcgcccca gcttttttca gcgcgctgac gtgagtggtc
    13921 agacccagcg ccgccatcgc cattgccagc aggaaggtat ccagcgttac cagcatgttc
    13981 accacgctct gcggtaacag gtggaacgag ttaaagatgg caactacgat gaacaagatg
    14041 gcaaaccacg gaatagtgat tttgcttttc tcgccgctgt tcgccccaga cagctgttta
    14101 acacgcgccg ccagcaggat gaggaacgga gccagcatca tcacgcgcag cattttggaa
    14161 ataactgctg cgttttctgc atccgggctg atggcatgac ctgccgccac cacctgcgcc
    14221 acttcgtgca cagtagaacc aatgtagata ccgaaagttt ccggactaaa ccattgagac
    14281 atcagcggat atatcgccgg gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt
    14341 gcaacagcca cggttacttt actggcttcc gctttcacta ccggctcagt cgccagtacc
    14401 gcggcagcac cacagatact gctaccggca ccgatcaacc aactggtgtg cttatccaga
    14461 ccaaacactt tctgccccag gaagcaagcc agcaggaagg tactggacag cgtcagcacg
    14521 tcaatgatga tcccactgat accgacatcg gcaatttgcg agaacgtcag acggaagcca
    14581 taaagaatga tacccagacg taataaatat tgcttggcaa acagcacacc accgtcacag
    14641 cttttccaga tgtgcggata gatggtgttg cctaaaacca tccccaacaa gattgcgagg
    14701 gtgagggcac taaacccggc acccgcaacc gcgggaatgg aaccacccca cagggcgacc
    14761 ccggtgataa ctgcactcag ggctaacccc ggaataaaat gccacagtgt acgatgttgt
    14821 ttctgtaagg tgatattcgt cataaccctc tcctttaacc gggcataagg ttacgactaa
    14881 ctggtttaaa aataaaattg attatatatt tataattaat ctttataagt ggtaagcgac
    14941 tatgcacatc accctccggc agttggaagt ttttgcagaa gtattgaaaa gtggatcaac
    15001 cacccaggcg tcggtgatgc tggcgttgtc gcaatcagca gtgagcgcag ccttgactga
    15061 cctggaaggg cagcttggcg ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa
    15121 tgaacacggg cggctgctct atccgcgagc gttggcactg cttgaacagg cggttgaaat
    15181 cgaacaactg tttcgcgaag acaacggcgc gattcgtatc tatgccagta gtaccatcgg
    15241 taactacatt ctgcctgcag ttatcgcccg ttatcgccat gattatccgc agttgccgat
    15301 tgaacttagc gttgggaaca gccaggacgt gatgcaagcg gtgctggatt tccgcgttga
    15361 tattggcttt attgaaggac cgtgccacag cactgaaatc atttctgaac cgtggctgga
    15421 agacgagctg gtggttttcg ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt
    15481 agaacagctg gccgctgcgc cgtggatcct gcgtgaacgc ggttccggca cgcgggagat
    15541 tgtcgattat ctgttgctgt cacatttacc gaagtttgag atggcgatgg aattaggtaa
    15601 ctccgaggca atcaaacatg cggtgcgtca tgggttggga attagttgcc tgtcgcgacg
    15661 tgtgattgaa gatcaattgc aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg
    15721 cctgatgcgt acgttgtggc gtatacatca tcggcaaaaa cacctttcca acgcgctacg
    15781 gcgctttctg gactattgcg atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca
    15841 tgctggtgct gtgtcgattt cgtgacgcag cgccttcagc atgcattcgc cagaaaagag
    15901 attggctgct ttacttataa tccctgggcg atcatgaagg tgtcttataa ccgtgtattt
    15961 ctgccggaag gattgccaat cgtctgctac aatcgcgcca tcatttttaa gatggatagc
    16021 atttttgtat gggttccgaa actaaaacta cagaagcgcc gggcttacgc cgtgaattaa
    16081 aggcgcgtca cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg
    16141 cctctggcgc aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgctga
    16201 ttggcctgat ggtttacttc ctgatgacca gtctcggtga attggctgca tatatgccgg
    16261 tttccggttc gtttgccact tacggtcaga actatgttga agaaggcttt ggcttcgcgc
    16321 tgggctggaa ctactggtac aactgggcgg tgactattgc cgttgacctg gttgcagcac
    16381 agctggtcat gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc
    16441 tcggcgttat cttcctgttg aactacatct ctgttcgcgg ctttggtgaa gcggaatact
    16501 ggttctcact gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga
    16561 ttatcggtat cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag
    16621 cgccgtttgc tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt
    16681 tccagggaac cgagctgatc ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca
    16741 ttccacgcgc ggtacgtcag gtattctggc gaatcctgtt gttctatgtg ttagcgatcc
    16801 tgattatcag cctgattatt ccgtataccg atccgagcct gctgcgtaac gatgttaaag
    16861 acatcagcgt cagtccgttc accctggtgt tccagcacgc gggtctgctc tctgcggcgg
    16921 cggtgatgaa cgcagttatt ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg
    16981 cgtctactcg tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta
    17041 aattgtcgcg tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt
    17101 tgtgcttcct gacctccatg tttggcaacc agacggtata cctgtggctg ctgaacacct
    17161 ccgggatgac gggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc
    17221 gcggttacgt attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc
    17281 cactggggcc gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg
    17341 aagcgttcct gaaagatacc attgactggg gcggcgtagc ggcaacgtat attggtatcc
    17401 cgctgttcct gattatttgg ttcggctaca agctgattaa aggaactcac ttcgtacgct
    17461 acagcgaaat gaagttcccg cagaacgata agaaataagt tttctccctt ccttgctaag
    17521 ccctctcaac cgagagggct ttttcaattc catttccctg acaaatcatg cggatataaa
    17581 atttaacatt tggattgata attgttatcg tttgcattat cgttacgacg caatcaaaaa
    17641 aggctgacaa atcagaggct gttccggctt tctgggatga tcacctgcat acaaataagt
    17701 ccaccgcgat gctgccgtac gcaaggggac gtaaagaaga tgtgagcgat acccatttta
    17761 ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtcg
    17821 ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg
    17881 aaacgatggt tgtcactgca tcttccgtgg aacaaaatct taaagatgca cccgccagta
    17941 tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc
    18001 tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta
    18061 ttcgtggtct ggacagcagc tacaccctga ttcttgtcga cggtaaacgc gttaactccc
    18121 gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg
    18181 aacgtattga agtggttcgt ggcccgatgt cgtcgctata cggctccgat gcgctcggcg
    18241 gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg
    18301 ataccaccat tcaggaacat cgcgatcgcg gtgataccta taacggtcag ttctttacca
    18361 gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg
    18421 aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaacgccg cgtattgaag
    18481 gattctccag ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt
    18541 ttactgccgg atacggtttc gaccgtcagg atcgtgattc cgactagctg gacaaaaacc
    18601 gcctggaacg ccagaactac tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg
    18661 aactgaaatc ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc agcccgataa
    18721 cttccgaaag caatacggtc gacggcaaat acacgttgcc gctgacggcg attaatcagt
    18781 ttctcacggt tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg aacctgaccg
    18841 ggggaaccag ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa gatgaatggc
    18901 ggatcttcga gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg
    18961 gtgaacactg gagtccgcgt gcctacctgg tttataacgc caccgacacc gtaacggtga
    19021 aagggggctg ggcgacggca tttaaagcac cttctctgtt gcaacttagc cctgactgga
    19081 cgagcaattc ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa
    19141 ccagcgaaag ttgggagctg gggctttact acatgggtga agaaggctgg ctggaagggg
    19201 gtgaatccag cgttatcgtt ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta
    19261 cgtctgacgt caacgctgca ccgggctacc aaaactttgt tggttttgag acgggcgcta
    19321 acggacggcg cataccggta tttagctact acagcgttaa caaagctcgt attcagggcg
    19381 tggaaaccga actgaaaatt ccgttcaacg atgaatggaa actgtcgatc aactacacct
    19441 acaacgatgg tcgtgatgtc agcaacggcg aaaacaaacc gctatccgat ctgccgttcc
    19501 atactgctaa cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg
    19561 tttctggtca ctataccggg cagaaacgct ccgacagcgc gacggctaaa acaccgggcg
    19621 gttataccat ctggaatacc ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg
    19681 caggcgtgct gaaccttggc gacaaggatc tcagtcgtga cgactacagc tataacgaag
    19741 acggacgtcg ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa
    19801 ccgggcggac tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa
    19861 agaggcgatg aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca
    19921 cgctttctgg cgcgcggctt ctgccaatac cgcaggttgc aactggtcgg caagaaactg
    19981 atcattatcg ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat
    20041 cagcgcgcaa ctatcccatt ccagccatgc atttttgtcc tcacctaaat aggtgctaaa
    20101 cgctttgatt ccccacggga cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct
    20161 ggtgtattta cccggatttt tcagcgccat aatcagcgca ccgtgaccac ccattgagtg
    20221 accgctaatg gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc
    20281 atcgcgcaga taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag
    20341 ataaaagcct gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg
    20401 cgggctggtg tctggcatca ccagtacaat ccccagttcc gccgctaccc gctgggcacc
    20461 cgccttggtg gtgaagttct cgtcattgca ggttaatccg gaaagccagt acagcactgg
    20521 tggcggggtg tgatcacgag ggggtgggag aaagatactg aacgtcatcg ggcagtttaa
    20581 ggtactggag tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag
    20641 catttccatg cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc
    20701 aatgtgctgt gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga
    20761 tgaaaggcag cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct
    20821 cacgcacaat aatcaagctg taaatcagct taataacttt gcccccacgc agggcggagg
    20881 cgtcacacct gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc
    20941 atgaagcgtt agttgcgaga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg
    21001 ataacgaaac gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga
    21061 atctcgacct ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg
    21121 tcgatgaaat tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa
    21181 acaaaatgaa ggtcgatgaa atggtcaccg tgcgcgatat cactctgacc agtacctgtg
    21241 aacaccattt tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg
    21301 tgatcggtct gtcaaaaatt aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc
    21361 aggaacgtct gacgcagcaa attcttattg cgctacaaac gctgctgggc accaataacg
    21421 tggctgtctc gatcgacgcg gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa
    21481 ccagtgccac gacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc
    21541 acgagtttct gcgcgctgtg cgccatcaca actgattaaa aggcaggaac catggagcgc
    21601 aacgtcacgc tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc
    21661 agcgcctttg ggctaccaaa ggcggcttat ctcaatcccg cctggtacga tgctattacg
    21721 ccgcaggatg catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg
    21781 ctttttgcgc tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg
    21841 atccagtcgc ggttaacgct gttagtcttg ttgggcttta ttcacggttt attgttctgg
    21901 gacggcgata ttctgctggc ttacgggctg gtgggcttaa tctgctggtg gctggtgcgc
    21961 gatgcgccat cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt
    22021 ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc ctggacgccg
    22081 gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc
    22141 agtaatcgtg ccgatggtgt tggcaatagt ttactggcac tgggcgcaca gtatggctgg
    22201 caactggcag ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg
    22261 cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc
    22321 attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc
    22381 ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg
    22441 ctgttttatg gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc
    22501 gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt
    22561 taccacctcg gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg
    22621 gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg
    22681 gtggaatggc tctggcgtca gttaactttg cgtgctgccg gactggcaat atctaaaaca
    22741 tcaagataac gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg
    22801 tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca
    22861 tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag
    22921 gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc
    22981 gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat
    23041 gcccaggcgc tggcaactca ggttagcgac accattggcg tggtggtgat ggacgtttct
    23101 gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa
    23161 tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg
    23221 ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag tgacgatgaa
    23281 ctggcacaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg
    23341 tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg
    23401 ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg cattgaagat
    23461 gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg
    23521 gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc gatggttgaa
    23581 ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct
    23641 ggcgcactga cagcattaaa agataatggc attgcgattc cgttacatct ctcaatcatc
    23701 ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac cgtgcgttat
    23761 cccattgctt caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat
    23821 attgatcctc gtgccgccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa
    23881 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
    23941 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaatg
    24001 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
    24061 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
    24121 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
    24181 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
    24241 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
    24301 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg
    24361 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
    24421 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
    24481 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
    24541 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
    24601 ttggcactga ctccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
    24661 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcagttcgta ctgctgaaag
    24721 gtgaaccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gaattgaacg
    24781 ataaaggcat caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg
    24841 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
    24901 tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt
    24961 ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg
    25021 gtgcactggc gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    25081 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    25141 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacttggct gaattcagca
    25201 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    25261 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    25321 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    25381 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    25441 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    25501 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    25561 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    25621 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    25681 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    25741 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    25801 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    25861 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    25921 aagttaccgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    25981 tggacaagat catcggcatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    26041 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    26101 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    26161 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    26221 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    26281 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    26341 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    26401 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    26461 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    26521 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    26581 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    26641 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    26701 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    26761 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    26821 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    26881 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    26941 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    27001 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    27061 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    27121 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    27181 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    27241 atggcatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    27301 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    27361 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    27421 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    27481 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    27541 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    27601 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    27661 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    27721 tcggcgtaaa cccatactgg cagtacatta tcaaaggggc gattattatc ttcgccgtag
    27781 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgcggatat tgccggatgc
    27841 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    27901 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    27961 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt
    28021 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    28081 acagccaaca catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    28141 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc
    28201 ctgatcggcg agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta
    28261 ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc
    28321 atcttcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    28381 ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    28441 ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    28501 ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc
    28561 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    28621 ggaaccccgt ttcactgccc gacaatattt ttttaccagc tccgggcttt gcccgacatc
    28681 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    28741 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    28801 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt
    28861 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    28921 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    28981 tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    29041 gcctaccggc gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat
    29101 cgaaagatct ttcgttaaca tgagcaagct ccctctaata tagtgatgaa tcgcctcggc
    29161 ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa caatatcgcc
    29221 agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg tgttacgctg
    29281 ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt
    29341 atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga ctttattgcc
    29401 ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggc
    29461 aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc aagttaccgc
    29521 ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat
    29581 aattaatgcg ctttgtggaa tgctgatatc gccttgcggc tgtcgtacac gttgcaggaa
    29641 gtcgacggca atctcaacgg tcgctatgct caaacagcgg tagcccggaa ccgcttgata
    29701 accccacggt gaccagtacc gcgcggtttt ccgctttcag ttgctccagg gtgagtgttt
    29761 taccgatttc gttattgcac ttaatggtca cgcccatttt ttcaatacgg gcgatctctg
    29821 catccagcac tgattgcggt aaccggaatt gcggaatacc gttacgcaac cagccaccgg
    29881 ggtgcgcttc tttctcataa atcgtgacgt cataaccctg gtttgtcagt gtcacactgg
    29941 cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt ttagtaccgg
    30001 gctgataaat ttccatcccg gtttgttgtt caaaatcagt aacaaaacgc tgtaagcggc
    30061 cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat aatttctccg
    30121 tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt gtctcggcag
    30181 cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg gtctgtgccg
    30241 ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct tctttaatcg
    30301 ccagtaacgg cgtaaaagcc ggagtgagtt catccagata attttgttgc ggcatgtgat
    30361 cttccttaat gctatgagta attatggcga acgctaaata aatattgcgt gctgatatta
    30421 aggctatgcg cgtggggaaa tttaaactgt gaaaggattc acaaatagct gcggttcaga
    30481 atgttatatc cgcagcttat tgagtgaagt aataattatt ctcaataatt aacagaaaag
    30541 agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
    30601 ccaatatatg ccaccatata agcgaagaag agtccgggat tttttgctcg ccagacatac
    30661 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
    30721 gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt
    30781 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtaacgg
    30841 cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc
    30901 ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
    30961 caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag
    31021 cctgaatccc catatgcagc gcaataaata atgctcgctc acagtggaaa cgttgggtga
    31081 taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac
    31141 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
    31201 tgcgcatggt catcggctcg ttataacttt gcaatgcgtt atcgccgctc agtaatagat
    31261 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
    31321 gattaataac gccggtacga taatattttg ctgtgccgag caccacgccg acctggcggt
    31381 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
    31441 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
    31501 gctttaacat gcaggagact cgagctaagg aaaatttcca tcaggctact ttacccgctg
    31561 gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc
    31621 aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcacgcggtc
    31681 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
    31741 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
    31801 attcaacaga attaacgccc cctgcaacgg cggcagagtc gggttgaatg cggcgttttc
    31861 agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggtga
    31921 cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc
    31981 accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc
    32041 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcaa
    32101 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
    32161 aggcgtatag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
    32221 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt gcaccaataa attccatatt
    32281 ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
    32341 caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc
    32401 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
    32461 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
    32521 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
    32581 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acacgtgaaa
    32641 cttacatcaa aaatgcgcga cgtatcgcaa attttagccc attactgcca gaataatcgg
    32701 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact
    32761 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
    32821 agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
    32881 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
    32941 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
    33001 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
    33061 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
    33121 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
    33181 gtttagcact tcgctacctt taaagtaact gtcgtaagag atgccggtca gcattaaaaa
    33241 aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc
    33301 cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt
    33361 cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgt
    33421 gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga aaccactacc
    33481 ggaccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
    33541 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca tttcgccgga
    33601 agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg
    33661 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
    33721 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
    33781 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
    33841 gcgcttattt tttcgctacc cggtattacg caatgagcag cgaactcagg catacatcaa
    33901 gcatagagag ggattgcagt ttgcagcaat ccctctttgc agacctttaa tccgctgacc
    33961 ataggcgcta acatagcgcc tcattttttt gcgggtgatg atatacgtgt gttactggca
    34021 ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac
    34081 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt
    34141 catcgcattt gccctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc
    34201 gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag
    34261 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt taacggtagc
    34321 ggcggcgggg cgacgttact caaagatcct gaactcatct accagggggc aaaagcgatg
    34381 cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag tgcgtctggg ctgggacagc
    34441 ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggcgctac ggagctggtg
    34501 gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
    34561 ggcgagattc gccagcggct gaatattccg gtgattgcca acggtgaaat ctgggactgg
    34621 cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
    34681 gcgctcaata ttcccaacct gagccgggtg gtaaaatata acgaaccgcg aatgccgtgg
    34741 ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg
    34801 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
    34861 gcaacggaat tatttc
//
LOCUS       DAJTYI010000026        34737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYI010000026 DAJTYI010000000
VERSION     DAJTYI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(26..1501)
                     /locus_tag="OO564_001266"
     CDS             complement(26..1501)
                     /locus_tag="OO564_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6652452.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(1648..2932)
                     /locus_tag="OO564_001267"
                     /pseudo
     CDS             complement(1648..2932)
                     /locus_tag="OO564_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3045..4979)
                     /gene="yeaG"
                     /locus_tag="OO564_001268"
     CDS             complement(3045..4979)
                     /gene="yeaG"
                     /locus_tag="OO564_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6652453.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5415..6161
                     /gene="mipA"
                     /locus_tag="OO564_001269"
     CDS             5415..6161
                     /gene="mipA"
                     /locus_tag="OO564_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6652454.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6251..7012
                     /locus_tag="OO564_001270"
     CDS             6251..7012
                     /locus_tag="OO564_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6652455.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7063..7947)
                     /locus_tag="OO564_001271"
     CDS             complement(7063..7947)
                     /locus_tag="OO564_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6652456.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8031..9026)
                     /gene="gapA"
                     /locus_tag="OO564_001272"
     CDS             complement(8031..9026)
                     /gene="gapA"
                     /locus_tag="OO564_001272"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6652457.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9368..9781
                     /gene="msrB"
                     /locus_tag="OO564_001273"
     CDS             9368..9781
                     /gene="msrB"
                     /locus_tag="OO564_001273"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6652458.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9823..10095
                     /locus_tag="OO564_001274"
     CDS             9823..10095
                     /locus_tag="OO564_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6652459.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10465..11541
                     /locus_tag="OO564_001275"
     CDS             10465..11541
                     /locus_tag="OO564_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6652460.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11568..12947
                     /locus_tag="OO564_001276"
     CDS             11568..12947
                     /locus_tag="OO564_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652461.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            12964..14009
                     /locus_tag="OO564_001277"
                     /pseudo
     CDS             12964..14009
                     /locus_tag="OO564_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14030..14866
                     /locus_tag="OO564_001278"
     CDS             14030..14866
                     /locus_tag="OO564_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6652462.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            14871..15819
                     /locus_tag="OO564_001279"
                     /pseudo
     CDS             14871..15819
                     /locus_tag="OO564_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            15829..16809
                     /gene="ydjG"
                     /locus_tag="OO564_001280"
     CDS             15829..16809
                     /gene="ydjG"
                     /locus_tag="OO564_001280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6652463.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            16946..17704
                     /locus_tag="OO564_001281"
     CDS             16946..17704
                     /locus_tag="OO564_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6652464.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            17822..19180
                     /locus_tag="OO564_001282"
     CDS             17822..19180
                     /locus_tag="OO564_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652465.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19273..19914)
                     /gene="pncA"
                     /locus_tag="OO564_001283"
     CDS             complement(19273..19914)
                     /gene="pncA"
                     /locus_tag="OO564_001283"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6652466.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(19925..20941)
                     /gene="ansA"
                     /locus_tag="OO564_001284"
     CDS             complement(19925..20941)
                     /gene="ansA"
                     /locus_tag="OO564_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6652467.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21108..22964)
                     /gene="sppA"
                     /locus_tag="OO564_001285"
     CDS             complement(21108..22964)
                     /gene="sppA"
                     /locus_tag="OO564_001285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6652468.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23125..23676
                     /locus_tag="OO564_001286"
     CDS             23125..23676
                     /locus_tag="OO564_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6652469.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            23793..24836
                     /gene="selD"
                     /locus_tag="OO564_001287"
     CDS             23793..24836
                     /gene="selD"
                     /locus_tag="OO564_001287"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6652470.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            24841..26802
                     /gene="topB"
                     /locus_tag="OO564_001288"
     CDS             24841..26802
                     /gene="topB"
                     /locus_tag="OO564_001288"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6652471.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            26929..27969
                     /locus_tag="OO564_001289"
     CDS             26929..27969
                     /locus_tag="OO564_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6652472.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFGEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28086..29429)
                     /gene="gdhA"
                     /locus_tag="OO564_001290"
     CDS             complement(28086..29429)
                     /gene="gdhA"
                     /locus_tag="OO564_001290"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6652473.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            29665..29937
                     /locus_tag="OO564_001291"
     CDS             29665..29937
                     /locus_tag="OO564_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6652474.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(29903..30310)
                     /gene="nudG"
                     /locus_tag="OO564_001292"
     CDS             complement(29903..30310)
                     /gene="nudG"
                     /locus_tag="OO564_001292"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6652475.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30397..31053
                     /locus_tag="OO564_001293"
     CDS             30397..31053
                     /locus_tag="OO564_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6652476.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ
                     "
     gene            complement(31062..32369)
                     /gene="ynjE"
                     /locus_tag="OO564_001294"
     CDS             complement(31062..32369)
                     /gene="ynjE"
                     /locus_tag="OO564_001294"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6652477.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32436..33089)
                     /locus_tag="OO564_001295"
     CDS             complement(32436..33089)
                     /locus_tag="OO564_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6652478.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33089..34623)
                     /locus_tag="OO564_001296"
                     /pseudo
     CDS             complement(33089..34623)
                     /locus_tag="OO564_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34596..>34737)
                     /locus_tag="OO564_001297"
     CDS             complement(34596..>34737)
                     /locus_tag="OO564_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6652479.1"
                     /translation="PQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYG
                     TH"
BASE COUNT         8811 a         8210 c         8861 g         8855 t
ORIGIN      
        1 agcatggctc tgaatatttt tgcacttaaa tattatgaat aatcactttg ttgcccccct
       61 cgcttttcgc ttcgcgtaag gctttatctg cttccgtcat gacaagcgag atattacgcg
      121 tgtcacccgt tgaaaaaaac gtgccaatac tgatggtcat ttgttcagga acatcatatc
      181 tgttactgaa gccggtcagg agttcgacat ttttacgcat acgttccgca atgatttttg
      241 cctgggcact attgagttcg gtaaatagca agccaaacac ctcaccctcc agtcgcgcta
      301 aaatatcatc ggggcggatg ctttgctgga taatatcgac aattgaaacc agcactttat
      361 cacccacacg atgtccccat tgggcattga tgcgtttgaa ctgattaata tcaaccagca
      421 tgacacagaa gtccttaaca acaggtcgcg acaataatga ctctacgctg ttgaaaaagt
      481 agcgccgatt ataaatattg gtcagcacat catgaactgc cagtttgctg gagagttgca
      541 gttcctgaaa aatgttataa atcagaaaag acacaacaaa cagtttactg gagacttcaa
      601 tcccgcgact gatgtaccag atggtgtaat tatactcatc cagagtcagt agaagtaagt
      661 tgcagcatag cgatgccaga cataataatg ctattaatgg ccatagttcg ctggctaatc
      721 gtgtacgcat gataataaag aacagtaaaa atgcccacag acaaaccaat atttttgtat
      781 agttgattcc ccaggtcgca gtatggttgt caggacagta atcgacgaca tacaaagaat
      841 agtcagcgtt ataactgctc agattgtgtg ccagaagggg aaaaactaaa aaaggaatca
      901 gcgccagcaa cagaatgcca gtttttttct tattgttgtc aaggatgttg tctttaccat
      961 aacaaaagag cgccaacgag gttaaacaaa taaaactgag ttggcggaaa atatagaaaa
     1021 tagaaacatc attcgacttt gtctggatca gtgtactgtc gctgataggt ttatggataa
     1081 caacaatagt ttcaactaaa tagataaggc cacttaaaag tgcaagactt aaaatgagtg
     1141 ttggtagaat ttgtttgtta gtgaaatatt tcattaacat gaaaagaaaa atgattgcgt
     1201 taaggaaaaa caaagccaca acgaccatca gataattcat ttgtgggatg agttttacat
     1261 tcgtcttttc gttatataat gtggtgatgc aaaataaaat taacgtcagc aataaactga
     1321 aaataaaaaa gctttgagct tttaattttt gtgtgcccat agaaaccata aaaaggagag
     1381 caatgtgagt gctggatttt attgatggca gagatatata gttttgacca agatcaattt
     1441 tcgttaacga taaaaaatat ttaaagaata accccaggga aatacgtgtt gactgaatca
     1501 tttttccgaa tgattgtttt ctggaactaa attgtgtaaa ggttatgctg tcatatttaa
     1561 agcgattgta agctaatgta tgtaataaat gagataattt acagtaagct aaaaatcagc
     1621 caggcgataa tgacccggct gatagtttta gtctttagct gttgcatttt gtttatgaaa
     1681 cagttcacgg aacaccggat aaatatcatc ctggtcgcgg atgtgctgca tcgcaaagtt
     1741 gtcgaaagta gattgcagat gctcatattc tcgcacacaa tgtctgatgt gcacgacggg
     1801 taatttcgat atagctgtaa taacgaacaa caggtaataa ttttttcgcc aggatttcat
     1861 ggcaaagcgg agagtcatcg gcccagttat cgccgtccga tgcttgtgca gcgtaaatat
     1921 tccactgtgc cgggttataa cgctctttca ctacctcatc catcagtttc agggcgctgg
     1981 aaacaatggt gccgcctgtt tcctgcgagt agaaaaactc atgttcatcg acttctttcg
     2041 cctgggtatg atggcggatg tatacgactt ccacgttctt atacgttctg ctgaggaaca
     2101 gatacagcag aatataaaaa cgcttagcca tatctttagt ggattgatcc attgaaccgg
     2161 aaacgtccat caggcaaaac atcactgcct ggctggaggg atcgggccgc ttctcgtagt
     2221 tcttgtaacg taaatcgaag gtgtcaataa aagggacgcg ttcaattttg gcacgtaatt
     2281 ctgcaatttc tttgcgcaga cgttcctctt ccagcagctg cgcaggttca ctgttgctga
     2341 tgatggccaa attctcttcc agtgcatgaa gttcccgccg cttgcctgcc gtcatggctg
     2401 tgcgtcgcgc cagtgagttc tgcaaggaac gcacaacgct gatattggct ggaacgccgt
     2461 tagcggtata acccgcccga tgcgttttat attcggtcag ctggcgttgt tggttttgtt
     2521 tcagattcgg taaggccaaa tcttcaaaga gcagatcaag atactcatct ttcgaaatct
     2581 gaaagacaaa ttcatcctga ccttcaccat cctggctggc ctggccctga ccactgccgg
     2641 aacctccgcc gccaccctgg ggacgttcaa ttcggtcgtt ctgaacgaaa tggtcattgc
     2701 ccggatgcac gcggtggcgc agaccgccac gcccctgatg aaacattggt tcgctaatat
     2761 cttccgtggg aatggatacg gattcgctgc tgtcgacgtc agtcaccgaa cgcttattaa
     2821 tggcctcgga gatcgactgt ttaatttgcg ctttataacg gcgtaaaaaa cgctggcgat
     2881 tcaccatgct tttgtttttg ccgttcagac gccggtcaat aaaccaggtc atatgccccc
     2941 cgtactacat ttgccaactg tatgctacgc ctgtgttgcc gcttctcagg cccacggttc
     3001 gctatcgttg taggcccggt agcatgtgta ccgggccagg gttgttaaga cgatttacgt
     3061 acgcgcaaat accattcgca cagtaaacgc acctgtttac gggtgtagcc tttttccatc
     3121 atacggtcga caaagtcgtc gtgtttcttc tgctcgtcgg ttgacgtttt ggcgttaaac
     3181 gagataaccg gcaacagctc ctcggtattg gagaacattt tcttctcgat aaccgtgcgc
     3241 agtttttcat agctggtcca gttcggattg cgtccgctgt tattcgctct ggcgcgcagt
     3301 acgaagttaa caatctcgtt gcggaaatct tttggattac tgatccccgc cggtttctcg
     3361 attttctcca gctcggcgtt aagagactcg cggtcaaaca gctgcccggt atccggatcg
     3421 cgatactcct gatcctgaat ccagaaatcc gcgtaggtaa cataacggtc gaaaatgttt
     3481 tgcccatatt cggaatagga ttcaaggtag gccgtctgga tctctttgcc gataaactcg
     3541 gcatatttcg ggatcagata acctttcagg aactccagat agcgttctgc ctgctcttgc
     3601 gggaactgct cgcgctcgat ctgctgttcc aggacgtaga acagatggac cgggtttgcc
     3661 gccacttcta catgatcgaa gttgaacacg cgggggagga tcttaaacgc aaaacgcgtc
     3721 gacagaccgt tcatcccttc atcgacaccg gcgtagtcac gatattcctg atacgacttg
     3781 gctttcggat cggtgtcttt cagactttcg ccatcataaa cccgcatctt tgaataaatg
     3841 ctggagtttt ctggctcttt caggcgcgaa agaatggaaa aacgtgacag tgtttcgagc
     3901 gtgccagggg cgcatggggc gtgagtcaat tcactgtgat taagcaattt ctcgtagatt
     3961 ttgatctctt cggaaatgcg caagcaatac ggcaccttca cgatgtaaac acggtcgagg
     4021 aaggcttcgt tgtttttgtt attacggaaa gtgacccatt cggactcgtt cgagtgggcg
     4081 agaataatcc cgttgaacgg cagggcggag ataccttccg tcccgttgta gttatcttcc
     4141 tgagtggcgg ttaacaaggg atgcagcact tttatcggtg ctttaaacat ctcaacgaat
     4201 tccatgatcc cctgattggc gcggcacagc gtaccggaat agccgtaggc gtccgggtca
     4261 ttctgcgcgt agtgttcgag tttacgaata tcgactttcc caaccagcgc ggagatgtcc
     4321 tggttgttct catcaccggg ttccgttttg gcgatagcaa tttgttgcag aattgacggc
     4381 cagaccttca ctacccggaa cttagtgata tcgccaccaa attcatgcag gcgttttgcc
     4441 gcccatggcg acatgatggt gccgagataa cggcgaggaa tgccatactc tttttccaga
     4501 atctgagcat cttcctgcgg attgaaaaga cagaacggat gatcgttgac cgggctacgc
     4561 tcaccgttgg cgctcaatac ataaatcggt acgagctgca ttaatgattt cagtcgctca
     4621 gcaagcgatg atttaccccc acccacaggc cccagcagat acaggatttg tttcttctct
     4681 tccagcccct gagccgcgtg tttcagataa gagacaatct gttcaatcgc gtcttccatg
     4741 ccgtaaaact cttcaaatgc cggataacgt gcaatgaccc ggttagaaaa gagtcgagaa
     4801 agtctgggtt cctgggctgt atcgaccatg acaggctcac cgatagccat caatagccgc
     4861 tcagccgcgt tggcataagc actgcgatct tgccgacaag tggtaagaaa ctcctgcagt
     4921 gtgaactctt cgtccttggc agcttcatat cgctggcgat agtgatcgaa tatattcatg
     4981 gtatgccgtc ctttcgtttt ttagcacagg ttaagagccg ttcatatgaa tagtagaggc
     5041 tcccggaaga ggtaaactga acgacgtctc ttgctaaaac acagcaactc tcatgccatt
     5101 ttatcgacga tgagaacaac gtgcccggtg atacaccttc aaaattaagc gtagatggca
     5161 aatggaaaac ttgcctgcac ggctttctta atttcaatgg catatcaaca actcatccat
     5221 aaattttgca taattaatgt aaagaccagg ctcgccagta acgctaaatc catttggctg
     5281 taagcgcggt gtcatccgcg gcaggaaaat taaacagtta ctttaaaaaa tgaaaacgta
     5341 aaaaggttgg gtttcgatgt attgacgggt aaactttgtc gcccgctaaa catttgttta
     5401 aggaatgatt aattgtgacc aaactcaaac ttctggcact tggagtgctt atcgcaacgt
     5461 ctgcaggcgt agcgcacgct gaaggtaaat tttccctggg cgcaggcgta ggtgtcgttg
     5521 agcacccata taaagattac gataccgatg tttacccagt accggtaatc aactatgaag
     5581 gcgataactt ctggttccgt ggcttaggtg gtggttacta cctgtggaat gacgcaacgg
     5641 ataaactttc aattaccgct tactggtcgc cgctttactt caaagcgaaa gacagtggcg
     5701 atcaccaaat gcgtcacctg gatgaccgta agagcaccat gatggctggt ctgtcttatg
     5761 ctcactttac ccagtacggt tacctgcgta ccaccctggc tggcgatacc ctggataaca
     5821 gcaacggcat tgtctgggat atggcttggt tgtatcgtta caccaacggt ggcctgaccg
     5881 tgactccggg tattggtgtg cagtggaaca gcgaaaacca gaacgaatac tattatggcg
     5941 tatcgcgcaa agagtccgct cgcagcggtc tgcgtgggta taacccaaat gacagctgga
     6001 gcccttacct tgagctgagc gccagctaca acttcctcgg cgactggagt gtttacggta
     6061 ccgcgcgcta cacccgtctg tctgatgaag ttactgacag cccgatggtg gataaatcct
     6121 ggactggcct gatttctacc gggatcacct acaaattctg ataatgcatc tttccagggc
     6181 attttgtgca taaaaatagg gcgtcacgcc ctgttttgca ttacggtggt gcaatacctg
     6241 ggaggaaaca atgcaacaaa aaatgattca atttagtggc gatgtctcac tgccagccat
     6301 agggcaggga acatggtata tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc
     6361 actacgcgca ggcattgaac tcggtttaac cctcattgat accgccgaaa tgtatgccga
     6421 tggcggtgcc gaaaaggtgg ttggggaagc attaaccggt ctgcgagaga aggtctttct
     6481 cgtctctaaa gtctatccgt ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc
     6541 cagtttacgc cgtctcaata ctgattatct cgatctctac ttattacact ggtctggcag
     6601 tttcgctttt gaagagactg ttgctgcgat ggaaaaattg atcgcccagg gaaaaatccg
     6661 tcgctggggc gtttctaacc ttggttatgc cgatatgcag gaactctggc agctgccggg
     6721 gggaaatcag tgtgccacta atcaggtgct ttaccatctc ggttcacgag ggattgagta
     6781 cgatctactc ccctggtgcc agcaacagca gatgccggtg atggcttaca gtccattagt
     6841 ccaggccggg cggttgcgca atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca
     6901 tgtccaacaa aatgcagctg cgcttgaggt cgaactttcg tcagcggaat tagctatgct
     6961 ggataaggca tatccggcac caaaaggaaa aactgcgctg gatatggtgt gatgttgata
     7021 cgtgccggat gcatcgcacc cggcacacaa cattaacgca aattaacgtt tggcaacgcg
     7081 aatggattgc gccagatgtg caggtttctc tttggtgact ttttgcgttt ctgaagcgta
     7141 agccgtttct acacaaacaa aggtcttgta gccatcgtcc ggcatgtcgc ccatgctaac
     7201 tgaaagcgcc ggtcccgggt tccagccgac aacgttcaga tgatgctggt ggcctacggc
     7261 gataatacga ttcagcgctt catcattaat cacgctgcaa tcttgcggat tcagatacac
     7321 gcgatcggta cggtcaggga aggtctgaat accgtcggtc agtacatctt ctttcgcgtc
     7381 attcacttta tcaatgaagc gatcgcccag cccactgacg cttaccttag cgatatcacc
     7441 cacgttaaag taggtatgca gggcagaggt ggtttcaaat tcgccatgtg attcaagatc
     7501 gatttcacag gttttaccca cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa
     7561 ttttttcgtc tcttcgcttt gtgtcaattc aaaagtcagc gctacgccat cagcatcttc
     7621 atgatgtgat ttcagcgtcc acggcaggtt gcgggcaaaa ccgtgcgcag gcagaccttg
     7681 ttgtgccgcc ggaccaaacc acggccagca aaccggtacg ctaccgcgaa tagcgacgcc
     7741 atttttgaac ggtgtgttgt tgctcaacca cagaacttct tcttcacccg caggtttcca
     7801 cgagagaagg tgtgcgccct gtaatgcaaa agaggctttt acctggggat gatcgaccac
     7861 aatgaggtcc agttcatcca gtttacgacg ggagaggaca ggggagattt gttcgatgac
     7921 cggaagggca aaaattttct taatcatgac gcagtccttt aacttcattt tatcaggtaa
     7981 aaaaaagagc gaccgaagtc gctcttttta gatcacagtg tcatctcaac ttatttggag
     8041 atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc gtaccaagat
     8101 accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa cacggaagtg
     8161 caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc cagaacgcct
     8221 ttcatttcgc cttcagcagc agctttaacg gcagctttga tctgctcgta agttgcagct
     8281 ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac gcggaacgcc
     8341 ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt agcagcaccg
     8401 gtagaggacg ggatgatgtt ctgggaagcg ccgcggccgc cgcgccagtc tttgtgagac
     8461 gggccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat cagaccttcg
     8521 atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt ggtggtgcag
     8581 gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc tttaacgaac
     8641 atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcacc agcggtgatg
     8701 tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac aacgtcaaca
     8761 cccacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg gattttttta
     8821 ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg
     8881 gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg
     8941 atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa
     9001 ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt gaataaaagg
     9061 ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg
     9121 acagatcaat cctgtgccta agcattacgc gactgactcg cctcactctt cctttgggct
     9181 tgagaccaca tggggtcggc gccccgaatt ttaaagggca attacgataa aaatgtgatt
     9241 ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa aattttgcca
     9301 gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta aagtgagatg
     9361 tgagtaaatg gcgaataaac cttcggcaga agaactgaaa aaaaatttgt ccgagatgca
     9421 gttttacgtg acgcagaatc atgggacaga accgccattt acgggtcgtt tactgcataa
     9481 caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc attccgaaac
     9541 caagtatgat tccggctgtg gctggcccag tttctacgaa ccggtgagtg aagaatccat
     9601 tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt gcggtaactg
     9661 tgatgcccat ctggggcatg tctttcccga cgggccgcaa ccaacaggcg aacgttattg
     9721 tgttaactct gcttctttac gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg
     9781 aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct tgatgacatt
     9841 atcaacagca tgacgcctga ggtataccag cgtttgtcga ccgccgttga actggggaaa
     9901 tggcctgatg gcgttgcgtt gaccgaggaa caaaaagaga attgcctgca actggtgatg
     9961 ctgtggcaag cccgccataa taccgaagca cagcatatga caattgacac aaatggtcag
    10021 atggtcatga agagcaaaca gcagttaaaa gaagattttg gtatctcagc aaagcctatt
    10081 gccatgttta agtaagtggt tcatcccctt tttgccgcgg ctatgaaggg gatagccaac
    10141 aatgacccca cgactcgtct caattatctc gacccgacct ggctaagtcc cagtccgatc
    10201 tcttgcagtc gcgttcacgc ctgaatgact tctcctgaac ttaagtctca gacctatttg
    10261 gccggtaatc cctctcgaat ttgctctttt tgcgtcccgc cttccaccaa cctgtaacag
    10321 acccagccat tttctgcgta attgtgatct aagttgtaaa atgcaggtca ttatctgtgt
    10381 tgtgcagatt taatgttgac ctcaaaaatc atgtgggtaa gtatgatggc catctaagca
    10441 aaacaaaaca cagaggatga cgcaatgaaa gcactggctc ggtttggcaa ggcctttggc
    10501 ggctacaaga tgattcatgt cccacaaccc atttgtggcc cggaagacgt agtgattgaa
    10561 attaaagccg cggcaatctg cggcgcagac atgaagcact acaatgtcga tagcggttct
    10621 gatgagttta actctatccg cggccatgag ttcgcaggtt gtattgcgca ggttggtgaa
    10681 aaagtcaaag actggaaagt ggggcaacgc gtcgtgtcgg ataacagcgg tcacgtttgc
    10741 ggcgtttgtc cggcctgtga acagggtgat tttctgtgtt gtacagaaaa agtaaacctt
    10801 ggtctggata acaatacctg gggcggtggt ttttccaaat attgtctggt tcctggtgaa
    10861 attctcaaaa ttcatcgtca tgcgttgtgg gaaatccctg atggtgttga ttatgaggac
    10921 gcagccgtac ttgaccctat ctgtaatgcc tataaatcca tcgcgcagca atcgaaattc
    10981 cttcctggtc aggatgttgt cgtcattggc actggcccac tcgggctgtt ttccgtacaa
    11041 atggcgcgga ttatgggggc ggtaaatatc gtcgtcgttg gtctgcaaga agatgtggcg
    11101 gtccgcttcc cggttgcaaa agaactgggg gcgacggcag tagtaaatgg ttctaccgaa
    11161 gatgtggtgg cgcgctgcca gcaaatttgt ggcaaagata atctgggact ggtgattgaa
    11221 tgctccggtg ccaatatcgc actgaaacaa gccatcgaaa tgctccgccc gaatggggaa
    11281 gtggtacgcg ttggaatggg cttcaaacct cttgatttct cgattaatga cattaccgcc
    11341 tggaataaaa gcatcattgg gcatatggcc tatgactcca cctcatggcg taacgctatc
    11401 cggctattag ccagcggcgc tatcaaagtc aaaccgatga tcacgcatcg tatcggcctg
    11461 tcgcaatggc gcgaagggtt tgatgcgatg gtcgataaaa ccgcaattaa agtgatcatg
    11521 acttacgact ttgatgaata actactctcc tgaaggaacg ttatcgcatg gaacagataa
    11581 caaaaccgca ttgtggtgca cggctggatc gcttaccgga ttgccgctgg cattcgtcaa
    11641 tgtttgccat cgtcgcgttt ggcttgcttg tctgctggag taatgccgtt ggtggcttga
    11701 tcctcgcgca gctgaaagcg ttgggctgga cagataattc caccacagcc acattctcag
    11761 caatcacgac cgccggaatg tttctcggtg cgctggtggg cggcatcatt ggcgacaaaa
    11821 ccggtcgcag aaatgcgttc atcctctatg aggccattca tattgcctcg atggttgtcg
    11881 gtgctttctc accgaatatg gattttctta ttgcctgccg ttttgtgatg gacgttgggc
    11941 tgggagcttt actggttaca ctgtttgctg gtttcaccga atatatgccc ggtagaaatc
    12001 gcggaacgtg gtccagtcgg gtttccttta ttggcaactg gtcatattcg ctctgctcac
    12061 tgattgcgat gggactcacg ccgctgatta gtgcagagtg gaactggcgg gtacaactgc
    12121 ttattcctgc aatattgtcg cttatcgcta cggcgctggc ctggcgctac tttcctgaat
    12181 ccccgcgctg gctggaatcg cgcggacggt atcaggaagc cgagaaagtc atgcggagta
    12241 tagaagaagg cgtcatacgc cagacgggta aacctttgcc gcctgtggtt attgctgatg
    12301 acggtaaagc gccacaagcg gtgccgtatt cagccttact gacaggagta ttactgaaac
    12361 gcgtgatatt aggctcttgt gtgctgatta ccatgaacgt tgtgcagtac acactaatta
    12421 actggttgcc aacaatattc atgacccagg ggattaattt aaaagactcg attgttttaa
    12481 ataccatgag tatgtttggc gcgccatttg gtatttttat tgccatgctg gtgatggata
    12541 aaattccgcg taaaacaatg ggtgtggggc tattaatcct gattgcggtg ctcggatata
    12601 tctattcact gcaaaccagt atgttgctca taacgctgat tggtttcttc ctgattactt
    12661 tcgtctatat gtacgtttgc tatgcctcgg cagtgtatgt ccctgaaatc tggccgacag
    12721 aggccaaact ccgtggctcc ggtctggcga atgcggtagg gcgaatcagt ggtattgccg
    12781 caccttatgc cgttgcagtg ctgctcagta gttatggtgt aacgggagtc tttattcttc
    12841 tgggggctgt ttcaattatt gtcgcaattg ctatcgccac cattggaatt gaaaccaaag
    12901 gtgtctccgt tgaaagttta agtattgatg cagtagccaa taaataatac gggtggattc
    12961 aaaatgaaaa attcaaaagc aatattgcag gtgccgggca caatgaaaat tatttcagca
    13021 gaaataccag tgcctaaaga agatgaagtt ttgattaaag tagaatatgt cggtatttgt
    13081 ggttcagatg tacatggttt tgaatcaggc ccgtttattc cgcctaaaga cccaaatcaa
    13141 gaaattggcc tgggtcatga atgcgccggg acggttgtgg ctgtgggaag ccgcgtgcgc
    13201 aaatttaaac cgggggatcg ggtaaatatc gaacctggcg ttccttgcgg tcactgtcgt
    13261 tactgtctgg aaggcaaata taacatctgc ccggacgttg attttatggc gacacaaccc
    13321 aactaccgcg gcgcattaac gcactatctg tgtcatccgg agagctttac ttacaaactg
    13381 cccgacaata tggacacgat ggaaggggcg ctggtggagc ctgccgcagt cgggatgcat
    13441 gccgcgatgc tggcagatgt taaaccgggt aagaagataa ttattctggg agcaggttgt
    13501 attggtttga tgacgttgca agcgtgcaaa tgcctgggcg caacggaaat tgccgtcgtt
    13561 gatgtactgg aaaaacgtct ggcaatgggg gaacagcttg gtgcgacagt ggttattaat
    13621 ggcgcaaaag aggacactat tgcacgctgt cagcaattta ccgaagacat gggcgcagat
    13681 attgttttcg aaacagcggg ttctgcggtc accgttaaac aggcaccttt aatctggtaa
    13741 tgcgcggcgg taaaattatg attgttggta ctgtacccgg cgattcggca atcaatttcc
    13801 tcaaaatcaa tcgcgaagtc actatccaga cggtattccg ctatgccaat cgttatccgg
    13861 tcacgattga agctatttct tcagggcgat tcgatgtgaa atcgatggtg acgcatattt
    13921 acgattatcg ggatgtacaa caggcatttg aagagtcagt taacaacaag cgcgacatta
    13981 ttaaaggcgt tattaaaatt agcgattaat taatacggga gaacagaaca tgctcgcaga
    14041 tatcagatat tgggaaaacg atgccacaaa taaacattat gcaattgccc attttaacgt
    14101 atggaatgca gaaatgttga tgggcgttaa agacgcagcc gaagaagcga aatccccggt
    14161 tattatttct tttggtacag gttttgtcgg taacacctca tttgaagatt tctctcacat
    14221 gatggtatca atggcacaaa aagcaacggt gccggtaata actcattggg atcatggtcg
    14281 gagtatggag attattcata acgcctggac tcatggtatg aattcattaa tgcgtgatgc
    14341 ttctgcattt gatttcgaag aaaatattcg tttaaccaaa gaggctgttg atttctttca
    14401 tccgctgggt attccggtag aggcggaatt agggcatgtc ggtaatgaaa ccgtttatga
    14461 ggaagcgctg gcgggctatc actataccga tcctgaccag gcggctgaat ttgttgaacg
    14521 tacgggctgt gactcattgg ctgtcgccat cggcaaccag catggggttt atacgtcaga
    14581 gccgcaattg aactttgagg tcgtcaaacg cgtacgcgat gctgtttctg ttccgctggt
    14641 tttgcatggc gcatcgggga tcagtgatgc cgacattaaa actgcaattt cgttgggtat
    14701 tgcgaaaatc aacattcata cggagctctg tcaggccgca atggtcgcag tgaaagaaaa
    14761 ccaggatcag cctttcctgc atttagaacg tgaagtacgt aaagctgtaa aagaacgggc
    14821 attggaaaaa attgaactgt tcggttctga tggcaaagcg gaataatgat atggataatc
    14881 tcgacgttat ttgtataggt gccgctattg ttgatattcc attacaaccg gtcagtaaaa
    14941 atatctttga tgtagattct tatcctcttg aaagaatcgc aatgagcacc ggggggcgat
    15001 gcaataaatg aagcaacaat tatttctcgt ctggggcatc gtaccgcatt aatgagccgc
    15061 attggtaaag atgcctcagg gcaatttatt ctcggccatt gtcgcaaaga gaatattgat
    15121 attcaaagcc tgaaacagga cgtcagcata gatacatcta ttaacgttgg actggtgacg
    15181 gaggatggcg agcggacgtt tgtcacgaac cgtaacggca gtttgtggaa attaaatatt
    15241 gacgatgttg attttgcgcg gtttcctcag gcgaaattat tatcactggc cagtattttc
    15301 aacagtcctc tattggatgg taaagcgcta acagaaattt ttacgcaagc gaaagcccgg
    15361 cagatgatca tttgtgccga tatgatcaaa ccgcggttga atgaaacgct ggatgatatt
    15421 tgcgaagcat tgagttatgt cgattatctg tttccaaatt ttgccgaggc aaaattactc
    15481 acagggaaag agacactgga tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg
    15541 gtggtgatta aaacgggtaa agacggctgc tttatcaagc gtggtgacat gacgatgaag
    15601 gtgccggcgg tcgcaggaat aaccgccatc gacaccattg gcgcgggcga taactttgct
    15661 tcaggtttta ttgcggcact gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc
    15721 aatgcaacgg cggctatctc ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga
    15781 aagctggtcg aacaattgct ggaagaatac gaaggataat gaaggcaaat gaaaaagata
    15841 cctttaggca caacggatat aacgctttcg cgaatggggt tggggacatg ggccattggc
    15901 ggcggtcctg catggaatgg cgatctcgat cggcaaatat gtattgatac gattcttgaa
    15961 gcccatcgtt gtggcattaa tctgattgat actgcgctag gatataactt tggcaatagt
    16021 gaagttatcg tcggtcaggc gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc
    16081 aaatgcggca ttgtctggga acgaaaagga agtttattca acaaagttgg cgatcggcag
    16141 ttgtataaaa acctttcccc ggaatctatc cgcgaagagg tagaagccag cttgcaacgt
    16201 ctgggtattg attacatcga tatctatatg acgcactggc agtcggtgcc gccatttttt
    16261 acgccgatag ctgaaactgt cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc
    16321 gctataggcg ctgctaacgt cgatgctgac catatccgcg agtatctgca atatggtgaa
    16381 ctggatatta ttcaggcgaa atacagtatt ctcgaccggg caatggaaaa cgaactgctg
    16441 ccgctatgtc gtgataatgg cattgtggtt caggtttatt ccccgctaga gcagggattg
    16501 ttgaccggca ccatcactcg tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg
    16561 ttccagcgtg aaaacatgct gaaagtgatt gatatgcttg aacagtggca gccactttgt
    16621 gctcgttatc agtgcacaat tcccactctg gcactggcgt ggatattaaa acagagtgat
    16681 ttaatctcca ttcttagtgg ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca
    16741 ctgaatatca acttatcgga tgcagacgca acattgatga gggaaatggc agaggccctg
    16801 gagcgttaaa tactcaacac gattcacctc tgtttctgtg aaaactgtga tgaaaatctg
    16861 tgattcaaat aggttatttt gaaagtaaac atcggttatg cgataatcgc gctaatgtga
    16921 tgtaaaaaga tacaggggtg taatcgtggc ggcaaaagac aggattcagg caattaagca
    16981 aatggttgcc aacgataaaa aggtgacagt ctcaaatttg agtgggattt ttcaggtaac
    17041 cgaagaaacc attcgccgcg atcttgagaa gctggaagat gaaggctttt tgaccagaac
    17101 ctatggtggt gctgttttaa atacagcggt gttgacggag aatatccatt tttataagcg
    17161 cgcttcatcg ttttatgaag agaagcagct tattgcacgc aaggcactac cctttatcga
    17221 caataaaacc accatggcag ccgattccag tagtaccgta atggaattgc tcaaattgtt
    17281 acaggaccgt agtgacctga cgttgctaac caactcggcg gaagcgattc atgttctggc
    17341 tcagtcagaa attaaagtcg tttcaacggg tggggaacta aacaaaaata cactttccct
    17401 gcaaggaaga attactaaag agatcatcag ccgctaccat gtcgatatca tggtaatgag
    17461 ttgtaaaggt cttgatatta acagcggcgc gctggactct aatgaagcgg aagctgaaat
    17521 caaaaagaca atgatccgtc aggcgacaga agttgcgtta ttggttgatc actctaagtt
    17581 tgatcgtaaa gcttttgtcc agttagctga ctttagtcat attaattata taataactga
    17641 taaatcaccg ggtgcagagt ggatagcatt ttgcaaagac aataatatcc agctggtgtg
    17701 gtaatttaag cacacgttgt caatattatt tgttcctgcg cctggaaata aaatgatgat
    17761 aaagatgcgg caagtgaatt tgccgcaatt gctacctttt atagcaaatc cgaggcaccc
    17821 aatggaacaa tatgatcaaa ttggcgcaag actggaccgc ttgcctttgg cccggtttca
    17881 ttatcgtata tttggtataa taagctttag tctgttatta acggggtttt tgagttactc
    17941 aggtaatgtc gtcttagcaa agctggtaag caatggatgg tcaaataatt tcctcaatgc
    18001 cgcctttacc tcggcattaa tgtttggtta tttcatcggc tcacttactg gtgggtttat
    18061 tggtgactac tttgggcggc gcagggcgtt tcgcataaat cttctcatcg tcggtattgc
    18121 tgcaacaggg gccgcttttg tccctgatat gtactggctc attttcttgc gcttcctgat
    18181 gggaacagga atgggggcgc tgattatggt tggctatgcc tcatttacgg agtttatccc
    18241 cgcgacggtg cgtggaaaat ggtccgcgcg gctctcattt gttggtaact ggtcgcccat
    18301 gctgtctgcg gcgataggcg tggtggttat cgcttttttt agttggcgaa taatgtttct
    18361 tctgggtggt attggcatac tgttagcctg gtttctctca ggtaaatact ttatcgagtc
    18421 gccacgatgg ctggcaggga aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt
    18481 agagcagcaa attgaaaggg agaagagtat tcgtttaccc cagcttactt tgaaccagag
    18541 caacagcaag gttaaggtaa tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt
    18601 acgacgtaca ttagttgcga taactgtttt aattgcaatg aacatttcgc tttataccat
    18661 taccgtatgg ataccgacca tatttgttaa ctccggcatt gatgtcgata aatcaatatt
    18721 aatgaccgct gttattatga ttggtgctcc ggtaggaata tttattgcgg cattaattat
    18781 tgatcatttt cctcgtcgat tatttggctc cgccttactt attattattg ccgtgttagg
    18841 ctatatctat tcaagtcaga ctacagagtg ggcgatttta atctatggtc tggtgatgat
    18901 cttcttttta tacatgtatg tttgcttcgc gtcggcggtt tatatcccgg agctttggcc
    18961 aacgcattta cgcctgcgcg gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt
    19021 tttcacgccc tatggcgttg cggcattatt aacacattat gggtcgatca cggtgtttat
    19081 ggtgcttggt gtcatgttag tgctctgtgc gctggttctc tccatttttg gcatcgaaac
    19141 gcggaaggtg tcgctggaag agatttctga ggtgaattaa atctttcgtt gatggcatca
    19201 attgccagat gtaccactga cgtgttttat ttggcctaca atgccagtac agaattgtag
    19261 gccagcgtaa aattacccct gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc
    19321 agctgctgac atctccataa acgcgtgtgc actgtcctgg ggctggatat tcacgccacg
    19381 acagccatcg gtaatcacgt ttaccttata acctaactgt aacgcgtcca gcacggtaaa
    19441 cttcacgcaa tagtcagtag ccaggcccat aacgatcaat tcatcgattt catgatcgcg
    19501 caaccagtca tcaagcgcgg ttttctgccg acgtccgtta tcaaaaaagg cactgtaact
    19561 gtcaactaaa ggattttcgc ctttatggaa caccgctgcg atctcttttt gattcagtaa
    19621 cggatgtaat tgtgcgcctt cactgttctg cacacagtgt tcaggccaga aagtttgccg
    19681 caggccgtcg agttggcctg gcgtataagg ctctacgccg tgctggctgg caaagctgcc
    19741 gtgattcgcc ggatgccagt cctggctggc gataaccgct tctccgcgcg actggcacca
    19801 gtcaataagg cggttagcaa cctccaccgt actgtcacct tccggtacgg cgagcgcgcc
    19861 accagcacag aaatcatttt gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt
    19921 ctccttaatc atccagcgtc agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg
    19981 tttcagtatc cagttcctgg ctcagcaggt aatgcagttt ggttagcgtg gcttctacag
    20041 tcatatctgc accgccaata acgccggcat gggcgaggac gttaccggtg gcataaccac
    20101 tcatattcac tttaccggac atacattgtg tcaggttgac caccacaata ccgcgatcgc
    20161 tggcttcttg taattcctgc aggaaggctt tgttttgtgg cgcattaccc acgccatagg
    20221 agcgcagaat caatgctttc accggttggc gcagaaaatt gcgcacgacg tcagcagaaa
    20281 tccctggata aatcgtcact acgccaattg gttgtggcgt gattggatga acgattaatt
    20341 ccccttcacc gtgcggggcg ggtggcgtat tcaaacgacg aatatggata ccagcttcca
    20401 gtaacggagg aaggtttgga gaggcaaacg catcaaaacc atcggcatgg gctttggtag
    20461 tgcggttgcc gcgatacaat cggttattga aaaagagcgt tacttcgttg atcggataat
    20521 tcgccgcaac gtacaacgca ttcagcagat taatttgtcc gtcagagcgt aactcagcca
    20581 gcgggatttg tgaccctgtc acaatgaccg gtttaccgag attctcgagc atgaacgaca
    20641 gcgcagaggc ggtatacgcc atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt
    20701 cgtcatagtg cgctttaata tcttcagcaa tatgctgcca gtcttccggc gtcatatcag
    20761 aagaatccat caacggcgta tattcatgaa tggtgaaatc tggcatctcc aggcgatgga
    20821 attccggcat cagcgccagt tggcgttgta gatgacctga caccggaata taaccctgct
    20881 cggaacgctg catcccgatg gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca
    20941 tgatattgat atgaatgata tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc
    21001 acctgatact atgcggataa ggaaatatgg ggcgagacag cgggaagagg attcgtcgag
    21061 gtaaaaaata atgtggccga cgatctgtcg gccactcagt acaagactta acgcacgttg
    21121 gcgcaggtca ggcaaaacgc ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg
    21181 ctttcacttt taacggtaga ggctaccgag gcaagcggtg caggtaacat ggcctggaac
    21241 gcatctggca acattgcccg gacagaacca gacatgttgt ccatcacttt gtcgaagaag
    21301 gtcggttcat caacgtagta ttccagatgc cactgtttca cttttgcgag ctctgctgct
    21361 ttggcaaccg catcatcgaa atccccgaga ctatcgacca gcccgttagc ttttgcatcc
    21421 tgaccggtcc agacgtggcc ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc
    21481 gcatcagcaa ccagcgtgat aaagcgttta tagccattct caatgcttaa ttgcatcatc
    21541 tgctgcgctt ccggcggcag tgccctggtg atagaaacat ccgccagcgg tgaagttgag
    21601 acaccatcag tatgaacacc aatcgaatcc agactatttt ctacggtggt gatcacgccg
    21661 aagataccga tagaaccggt cagggtgctg gggttagcca caatgtaatt agctggcgtg
    21721 gaaatccagt aaccaccaga tgccgccatg ccgcccatcg atacaaccac agacttaccc
    21781 gctgcccggg ctgctgccag ttcagcgcga atcacttcag acgcggtaac gctgccgcct
    21841 gggctattaa cacgcaggac aatcgctttc actttcgggt caaggcgagc gtcgcggatt
    21901 tgtgccgcag tggtatcacc gccaacattc ccctgagttt cctcgccatc cataattgcg
    21961 ccattagcga agacgacacc gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa
    22021 tcgtaataac tgatggcgcg ataattttta tcagtcttac tccagccgaa ctctttggtc
    22081 agagcttttt cgatttcggc actcgatgcc agtgcatcga ccagcttgtt ttccagtgca
    22141 tatttcgcgg tatcgccacc ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg
    22201 aatacctgct gagcagggat ctgccggtta gcggcaacag tattcagata gttttgccac
    22261 agctcaccaa tccagcggct gtcagcttcg cgggctgccg gtgacatatc atcacgaata
    22321 aacggttcaa cggcagattt atacgtaccc acgcggaaca catgggtgga aactttcagc
    22381 ttatccagca acgatttgta gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg
    22441 ccttgcggtg acagccaaat tttattggcg aaactggcga gataatattg cccctggctg
    22501 tagttctcgc caacggcata aaccggtttc ccgctgtcac gaaactcttt cagggctttg
    22561 ccgatgtact gcatagacgg ctggtcgccg cctgcgaagt ttttcagatc catcacgata
    22621 ccggtgatat tgcggtcgtc cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag
    22681 ttttcctgca gacgatcgga actggcacca agcagctggc ggcttaattt actaaaccgc
    22741 tgagaactgt ctggtttatc gacgatcaca ccagaaatgt ccagcagcag tgcgccacga
    22801 ctggccgttt ctttcgaatc accaccactg acctgcatcc agatccccac accaaccagt
    22861 acgaggaaaa taaagaacag gttaagtacc atttcacgga cgaaattcag cagacgccac
    22921 gtccatttaa aaaatccggc aataaatcgc caaagggttc gcatgtattc tcccaactta
    22981 aggtcacacc tgtcacaggc gcaatagatg tggttatcgt aatgacccaa ccggcacttg
    23041 tcagcaggaa ttgcggactg tgctgtaaca aaatccactc tcgtgttaat tttgtgaata
    23101 aatatcacga caggagttaa tcaaatggat gcactcgaac tattgatcaa tcgccgtagc
    23161 gcctcccgct tggctgaacc cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg
    23221 ggtatgcgtg cgccggacca taagtccatg caaccgtggc atttttttgt gattgaaggg
    23281 gaagggtgcg agcgtttcag cgccgtactg gaacaggggg cgattgctgc cggtagtgat
    23341 gataaagcta tcgacaaagc ccgtaatgcg ccgttccgcg caccgctcat catcacggtg
    23401 gtggcgaaat gcaaagaaaa tcataaagtc ccgcgatggg aacaggaaat gtctgccgga
    23461 tgcgcggtca tggcgatgca aatggcagcc gttgcccagg gatttggcgg catctggcgc
    23521 agtggcgcat taactgaaag tccggtagtg cgtgaagcat tcggttgccg tgagcaggat
    23581 aaaattgtcg gttttctcta cctcggcacg ccgcagttga aagcatctac gtcgattaac
    23641 gtcccggacc cgacgccgtt tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat
    23701 tgtgagcaaa ggcgcaggaa ttcagacact ctcacttatc acttcacgga atgagggtta
    23761 ccatacccgg attgcattga caggagatgt ccatgagcga gaactcgatt cgtttgaccc
    23821 aatacagcca cggagctggt tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc
    23881 tgcacagtga gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg
    23941 acgatgcggc ggtgtacgat ctgggcaatg gcaccagcgt tatcagtacc accgacttct
    24001 ttatgccgat cgttgataat cctttcgatt ttggccgcat tgcggcgact aacgccatca
    24061 gcgatatctt cgcgatgggg ggcaaaccga ttatggcgat tgcgatcctc ggctggccga
    24121 ttaacaaact ttccccggaa attgcccgcg aagtaaccga aggtggacgc tatgcatgcc
    24181 gtcaggcggg tattgcgctg gctggcggtc actccatcga tgcgccggag ccgatttttg
    24241 gtctggcggt aacggggatc gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag
    24301 ccggatgcaa actgttcctg acgaaaccgc tggggatcgg cgttcttacc acggctgaga
    24361 aaaaatcact gttgaaacca gaacatcagg gactggcgac ggaagtgatg tgccggatga
    24421 acatcgcagg cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg
    24481 gctttggtct gctgggccac ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg
    24541 tcgactatga agcgatcccg aaactccccg gtgttgaaga gtacattaag ttgggcgcag
    24601 tacctggcgg cactgaacgt aactttgcca gctacggtca tctgatgggt gaaatgccgc
    24661 gtgaagtgcg cgatctgctg tgtgatccgc aaacttctgg cggtttgctg ctggcggtca
    24721 tgccggaagc agaaaatgag gttaaaacta cagccgccga gtttggcatt gaactgacgg
    24781 cgattggcga actggtgcca gcgcgcggcg gtcgtgccat ggttgagatc cgttaattca
    24841 atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc
    24901 aaaccgcacc ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacttgg
    24961 tgtatcggtc acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc
    25021 tggaatcttg cggatttgcc gattgtcccg gaaaagtggc aattacagcc acgaccctcc
    25081 gtgaccaaac aacttaacgt catcaaacgc ttcctgcatg aagccagcga aatcgttcac
    25141 gccggggacc cggatcgtga agggcaattg ctggtggatg aagtgctgga ctatctgcaa
    25201 ctggcaccgg aaaagcgcca gcaggtgcag cgttgcttga taaacgacct gaacccgcag
    25261 gcggttgagc gggcgatcga ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt
    25321 tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat
    25381 accattctcg gtcgcaatgc cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca
    25441 cccgtgctcg ggctggtggt gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac
    25501 ttctttgaag tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg
    25561 caaccgagcg aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca
    25621 ctggcggagc atgtggttaa ccgcattagt ggtcaaccgg ctattgtcac cagctataac
    25681 gataaacggg aatcagaatc cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa
    25741 gcggcaaaac gcttcggtct gagcgcgcag aacgtgcttg atatctgcca gaagctgtac
    25801 gaaacgcaca agctaatcac ttatccgcgt tccgattgtc gctatttgcc agaagaacat
    25861 tttgccggac gccacgcggt gatgaatgcc atcagcgttc atgcaccgga tctgttgccg
    25921 cagccagtgg tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg
    25981 caccacgcca tcatcccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg
    26041 aaggtctata acctgattgc ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc
    26101 cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt
    26161 cttgctgaag caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac
    26221 ggcacgccat tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg
    26281 gtagagcggc aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg
    26341 accgggatcg cgcgctttgt gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat
    26401 ggtctgggga cagaagcaac ccgtgccggg attattgaac tgctgttcaa gcgtggtttt
    26461 ctgaccaaaa aagggcgcta tatccactcc accgacgccg gaaaagcgtt attccattcg
    26521 ctgccagaaa tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa
    26581 atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacgctatat
    26641 cagcttattg atcaagccaa acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg
    26701 ggcagtggtg gcagtgctga taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa
    26761 agtccgccag cagatgaagc cggaagcggg gcgatagcgt aagcgagcga atctttcgtg
    26821 ctattcgagt catattctga aatatccagc ggatcaagaa aattcgttgg atattttttt
    26881 gcatggataa aattatcgcc tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt
    26941 ttattacaaa accatcctgg gagcgagaag tattctttta atggctgggg aatatttaat
    27001 agtaattttg aacgggtgat taaagaaaat aaggccatgc tgctttgtaa gtgggggttt
    27061 tatttaacat gtgttgtcgc tgtaatgttt gtattcgcag cgataacatc caacggtttg
    27121 aatgaaagag gcctgattac cgcgggatgc tcttttcttt atctattaat tatgatgggg
    27181 cttattgttc gggccggttt taaagcaaaa aaagaacaac tacattatta tcaggctaaa
    27241 ggtattgagc cgctcagtat cgagaagtta caggcgctac aattgatcgc accttatcga
    27301 ttctatcata agcaatggtc tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc
    27361 aaagatacct tccaatatca tgtgcttcct tttgactcga tcgatatcat cagtaaaaga
    27421 cgcgagtctt tagagaatca attgggtatc gaagatagcg aaagttattg tgccttaatg
    27481 gagcattttc tttctggcga ccatggagcc aataccttta aagcaaacat ggaggaagcc
    27541 ccagagcagg ttatcgccgt gttgaataaa tttgctgttt ttccctcaga ctatatctct
    27601 gattgcgcta ataatcgctc cggtaaatcc tcggcgaagc taatatgggc ggcggaatta
    27661 tcatggatga tctcgatatc aagcacagct tttcaaaacg ggacaattga agaagaactg
    27721 gcctggcatt atataatgct tgcttctcga aaggcgcacg agttgttcga aagcgaagaa
    27781 gattatcaaa aaaatagtct aatgggattt ctttactggc atatctgctg ctatcgcaga
    27841 aagttaacgg atgctgaact tgaagcgtgt tatcgttacg acaagcagtt tggggagcac
    27901 tacagtaaga aatgccgttg gcccataaga aatgttccct ggggagcatc atccgttaaa
    27961 tactcataaa cgcctgaaat tttgccgggg gcgctgcgct tgcccggcct acattagagc
    28021 gtaactgcaa tgaattgtgc ccatctgtag gcctgataag cgtagcgcca tcaggcattt
    28081 acaacttaaa tcacaccctg cgccagcatc gcatcggcaa ccttcacaaa accggcaatg
    28141 ttcgcgccct gcacgtagtt ggtttgctca ccttcaccac catgctcaac acaggcatgg
    28201 tggatatcca gcatgatgtg atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg
    28261 cgcgcagcgt tttgtgccat ttccaggccc gacgttgcta caccgccagc attagccgct
    28321 ttacccggtg caaacagtac gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc
    28381 ggcatatttg ccccttcagc gacggcttta acgccattgg cgataagctg atgcgcggtg
    28441 tcaacatcca gttcgttctg ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac
    28501 ggctgttggc cttcgagata gaccagacca aattctttgg cgtaatctgc cactcgacca
    28561 tcgcggctgg ctttgatttc gataagacgt gccagtttct ctttcgtgaa tccgctttca
    28621 tcaactacag tgccgctgga gtctgacgca gtgatcacac gagcaccaaa ttccatcgct
    28681 ttttcgatag cgtactgggc gacgttgccg gagccagaaa cggaaacgcg catcccttca
    28741 aaacccatac cgtggcgttt taacattgct tctgtgaaat aaaccagacc gtagccggta
    28801 gcttccgggc gaataagact accgccaaat gaaaggccct taccggtgaa gacgcaggcg
    28861 gtattgttgg agagcttttt catcatcccc gccataaagc cgacttcacg accaccaacg
    28921 ccgatatcac ctgccggaac gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc
    28981 agcgcctggc aaaaacgcat cactttacct tcgctttttc ctttcggatc gaaatcgctg
    29041 ccgcctttac caccgcccat cggcagggta gtcagggcat ttttgaaggt ttgttcaaag
    29101 ccgaggaatt tgagaatgga aaggttaact gacggatgga agcgcatacc gcctttgtac
    29161 gggccgatgg cagagctgaa ctgcacacgc catgcacggt tgacctgtat ctggttgcga
    29221 tcatcaaccc ataccacgcg aaactggatc acgcgctccg gttcaaccag acgctccagt
    29281 aatgacatct ggcgatattt tggattttgt tcaagaaaag gccagagtgt ggtcattact
    29341 tcacgaacgg cttgcgcgaa ctcggtttga ttcgggtcgc gcttttggac atggttgagg
    29401 aatgactcca gagaatatgt ctgatccata gatataaaaa ccttatatat taatacgatt
    29461 gtgcttatgt tgtttatgtc atgtgctttt gcagttttcg actataccat caagaatgtg
    29521 acggtaagca agaaaaaaat gacccaggaa agcaaattaa taacgagagt aatctcataa
    29581 ccaaatgcga taactcagac gctaaagttt tgccacccgg ctaccgttat cagtagcaga
    29641 gacaataacg ttaaggagtt ccctgtgagt cgagcattgt tcgccgtagt attagcattt
    29701 ccattaattg cactggctaa cccgcattat cgtccggacg tggaggtaaa cgtaccgcca
    29761 gaagtgttca gttcaggcgg gcagtcagcg cagccctgta ctcagtgctg cgtttatcag
    29821 gatcaaaatt attctgaagg ggcggtaatt aaagccgaag gaatactgct gcaatgccag
    29881 cgtgatgaca aaacccttag cactaatccg ctgctctggc ggcgcgtaaa gccataaacg
    29941 cctctaataa tggaatgtca gcaggggcca gcggatattg cagcgcctct tcaggtgagc
    30001 accagaccag cgcctgatgt tcatgtgcct gtaacgtccc gtggaagtcg ggtacgtgcc
    30061 aggcatgaag atggataatc cggcccgaaa cttctcgctg atggctggca acatactcac
    30121 ccacagttgc ttcgatgtcc aattcttcgt ttaactcacg caccagcgcc tgctgctggc
    30181 tttcatccag ctcgacttta cctccggcaa actcccataa tcccgcctga tcgctctggg
    30241 cggggcgttg cgcgagtaaa attttgccat cacgttcaat gatggcggca acaacttcaa
    30301 tcattttcat aaattacaca cgggcagata agcgaaaaat gccataatat ccgcactgtt
    30361 gctcaatcag aaaggcttta tatttaagga gtgcctgtgc tagaccgcca tcttcatccc
    30421 cggattaaac cgttgttgca tcagtgcgtg cgggttcttg ataaatcggg cattacgcca
    30481 gatggtttaa cattagtggg atttgccatc ggcgtgctgg ctctgccgtt tctggcgctg
    30541 ggctggtatc tggcggcgct agtcgtcatt ttgttgaaca ggctgcttga tggtctggac
    30601 ggtgcgctgg cgcggcgcag agggcttacc gacgcgggcg gctttcttga tatttctctc
    30661 gattttctct tttacgcgct ggtgccgttt ggctttattc ttgctgcacc ggagcaaaat
    30721 gcgctggcag gcggctggtt gttgtttgcg tttatcggca cgggcagcag ttttctcgcc
    30781 tttgctgcac tggcagcaaa acatcagatt gataaccccg gttatgcaca taagtcgttt
    30841 tactatctgg ggggattaac cgaaggcaca gaaacgatct tactgtttgt gctgggatgt
    30901 ttattccctg catggtttgc gtggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg
    30961 tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct ttacgcgggt gtggagtggg
    31021 tatctgacgc tgaagtcgct ccagcgtcag taattgcaat gttatttgct actgcccggg
    31081 ccgcgttcac cggttgcaac cggatttttt ggatcgctgc tccattcgta ccagccgccg
    31141 tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt acataaaggt ttcggacgcg
    31201 cgccagccgg tgccgcagta gaatgaaact tgctgatctg gtttgatatt ccatgctttc
    31261 cacatagcgg taatatcatc ggcgctacgc atggtgccat ccgggttatg gaaatcttcc
    31321 atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc cggctatttc accttttggt
    31381 ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg gccacgaacg aatgctcacc
    31441 agcgatgcat cctggcgatg cagcagtcca cgggcctgtt ccatatcgag catcaactgc
    31501 ggttgtgccg ggatcttcac gccgaaatct ggttccgctt tcactttcgg tggcgttcct
    31561 cgctcaacag gcagtcccgc gtcggaccag gtttgccagc cgccatccag cagacgcaca
    31621 tctttcacgc ctgcgtagag cataatttgc gccacacgcg ctccagcgta tacgtcacgc
    31681 ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt ttgccagcat cgcttttagt
    31741 tgttcatcag aaactttgtt ccacagcggt tcgctttcca cttcgttggt atcgatgtag
    31801 tcagcgccag gaatatggct gataaggtaa agcttaggag cgccccaggc cgcttcaatg
    31861 actttccagc caccgacagg tttcgccgta acctctttac cttgttgcag gtcatgcagc
    31921 cattgcggat aaaccagctg ctcaaagtgc ggcagttttt gtagacggga aggttcgctt
    31981 agcgcgtcac tcagaatgga gatatgcgta aaacctgctt tttgcagtcg cgttttgacg
    32041 gcgtcgacat ctttgccatt accgtacagc gccaccgggg catcggcttt caggttatgt
    32101 tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt cgagccagct agcagagagg
    32161 tttaaggcgg caggttcatg accagaaggg ccatttaagg tttgtggcca gccgttataa
    32221 aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt gctgaagttg gtcaagtgta
    32281 agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag ccagccctaa agccattgcc
    32341 agcgcggtca tttgagaaac acgtttcatc gaaaatcccg acattatagt tttgagtgcg
    32401 ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc gcagtttgtt gtaattttct
    32461 gaccactgcg ccatatccag aacagaacta tcagcaggaa catcctggag atcgtgcgtt
    32521 acctgaacga cggggatcgc cagggcgcga acttcgctga acacccactg gcgaaaatta
    32581 tcgcgcagag ccacatcaag acggctgaat ggctcatcca ggagtaacgc ttttggctgg
    32641 gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac cgccagacaa agtggcagga
    32701 tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg catcattcac ggcattacgt
    32761 cgggcattcc ccttaagtgt cgccggtagc gccagcagta aattttgccc gacgctgaac
    32821 tggtcaaata acagtgcatc ctgaaaaaga atgccaatct gacgctgtgc ggtgggtagc
    32881 atgtcaatcc gttgctcatt gagccatagc tcacctgtac aagaaaactg tccggccagt
    32941 gcaccaatca tccatgaaaa cagagtggat tttccacagc cagatggccc cattaacgtg
    33001 acgatgtcac ctttatcaac cgtaaagtta acgtttgtca gcaagcggct ttctggtaaa
    33061 cgtagcgaaa catttttcac gcagagcatt agcggattcc ttgtctgaca taacctaccc
    33121 attttgcgac taacgcggtc agggcaaaaa taataagcgg taacaacagt tgccataaag
    33181 cctgggcggc gagaataccg ttgctgccgc cgctgcttaa tgccaccgcc tcagtggtga
    33241 gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat atactgcgca atactgactg
    33301 aaaatcccac cgcgaaggca atcagcacag ggcgcaacat gagtgggcat ttcacgtaaa
    33361 agaagatttt ggcccgcgac cagcccagtg tttgcgcaat caaaattaac cgtgaatcaa
    33421 tgcgctgcca ggcgggttgc aggataaaca gcatccacgg cattacccac agcagatgcc
    33481 cccagaccac cgccgtccag cttccatcca ggttcagcca tagcgccagc gtgtactagc
    33541 ccgccaccag tggcagagcg ggcaatagaa tgggcaacca tagccacaac tggcgacgct
    33601 gtgcccccca ttccagccac agcagtaaca ggagcaaagc gatgaatgtt gctaccagcc
    33661 ccattgtcag actgttgatg agtgcttcac tattgatcgt cgactgatcc gcgaggatcg
    33721 ccagcagaac cacacacagc acaccggtta agggtaaaaa actcgccagc gtagtgcctg
    33781 gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg gggaatagtg cgccgccagc
    33841 tgcgccatag cagatagctc agcaaaacgt aggcggcgag taacagcatc agcagcagac
    33901 tggcgagtgc gcctttcgtt tgttgatccg cgtcgccctg agttaaccac tgccagctaa
    33961 ttactgccag cgtcggtgga ttacctggcc caagaataat cgccacatcc acgaccgaaa
    34021 gcgaccaggc aacaatcgcc agcatcgcca ttgccagcga aggcgcgacg gagggcaaca
    34081 gcagccagtt caggcattgc cagcggctgt agcccagcga atccagcaca atgacctgct
    34141 gcaacagccg tttttcgctc aatactgcca ctaagatcca cagcagaaat gcgctttctt
    34201 tcactgcaag ggtgaggccc agaccgatcc aaatcggtcc attggcggag tgaaatacgg
    34261 gaaatagtca tatagcagcc ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc
    34321 cacatgggga atggcgagca gccacggcag acgggcgcac atacgctgcc atttcggccc
    34381 aggccatagc gccacaatca ccagcagggc aatcaacaat gccccgaccg ccgcgatggt
    34441 tgtcgacacc agcgttgcca gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg
    34501 ccagcgtgtc aacgacaacg caggcgatat cagcgtgaga gctgccggga tcagcggtgc
    34561 ataaatcacc gccaccatcg cccacagcaa aaagattaat gcgtaccgta acggcgtaac
    34621 cattcttgtt ccagtgcatt cacccatcct gcgtgcggtt cagccagtac cggcggcaga
    34681 tcctgcggca ttcttgattg caatatttcg cgctgcccgt caggcagttt ttgcgga
//
LOCUS       DAJTYI010000027        34671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYI010000027 DAJTYI010000000
VERSION     DAJTYI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO564_001298"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO564_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6652480.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO564_001299"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO564_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6652481.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO564_001300"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO564_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6652482.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO564_001301"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO564_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6652483.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO564_001302"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO564_001302"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6652484.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO564_001303"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO564_001303"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6652485.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO564_001304"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO564_001304"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6652486.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO564_001305"
     CDS             complement(4598..5320)
                     /locus_tag="OO564_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6652487.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO564_001306"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO564_001306"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6652488.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO564_001307"
     CDS             6520..6738
                     /locus_tag="OO564_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6652489.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO564_001308"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO564_001308"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6652490.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO564_001309"
     CDS             complement(7472..7672)
                     /locus_tag="OO564_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6652491.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO564_001310"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO564_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6652492.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO564_001311"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO564_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6652493.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="OO564_001312"
     CDS             10169..12082
                     /locus_tag="OO564_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6652494.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="OO564_001313"
     CDS             12082..13104
                     /locus_tag="OO564_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6652495.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="OO564_001314"
     CDS             13098..13316
                     /locus_tag="OO564_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6652496.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="OO564_001315"
     CDS             13370..14239
                     /locus_tag="OO564_001315"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6652497.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="OO564_001316"
     CDS             complement(14294..14698)
                     /locus_tag="OO564_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6652498.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="OO564_001317"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="OO564_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6652499.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="OO564_001318"
     CDS             15683..17773
                     /locus_tag="OO564_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6652500.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="OO564_001319"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="OO564_001319"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6652501.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATLEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="OO564_001320"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="OO564_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6652502.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="OO564_001321"
     CDS             complement(19741..20343)
                     /locus_tag="OO564_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6652503.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="OO564_001322"
     CDS             complement(20333..20500)
                     /locus_tag="OO564_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6652504.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="OO564_001323"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="OO564_001323"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6652505.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="OO564_001324"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="OO564_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6652506.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="OO564_001325"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="OO564_001325"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6652507.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="OO564_001326"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="OO564_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6652508.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="OO564_001327"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="OO564_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6652509.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="OO564_001328"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="OO564_001328"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6652510.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="OO564_001329"
     CDS             28328..28495
                     /locus_tag="OO564_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6652511.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30127
                     /gene="frlA"
                     /locus_tag="OO564_001330"
                     /pseudo
     CDS             28790..30127
                     /gene="frlA"
                     /locus_tag="OO564_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="OO564_001331"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="OO564_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6652512.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32050
                     /gene="frlC"
                     /locus_tag="OO564_001332"
     CDS             31220..32050
                     /gene="frlC"
                     /locus_tag="OO564_001332"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6652513.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32047..32832
                     /gene="frlD"
                     /locus_tag="OO564_001333"
     CDS             32047..32832
                     /gene="frlD"
                     /locus_tag="OO564_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6652514.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32932..33663
                     /gene="phoB"
                     /locus_tag="OO564_001334"
     CDS             32932..33663
                     /gene="phoB"
                     /locus_tag="OO564_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6652515.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33823..>34671)
                     /locus_tag="OO564_001335"
     CDS             complement(33823..>34671)
                     /locus_tag="OO564_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR6652516.1"
                     /translation="RAGLAALLKPGQRLLVHDAPVYPTTRVIIEQMGLTLITADFNDL
                     LALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATLRAAGVPALTDDNYAVMKVARI
                     GCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRATLYSGGSQIQGSQALEVLRGL
                     VFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANAQSKVLIVEFHQLIAARVLEEA
                     QKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLEHCAIRINPNRSGEETVLRILR
                     ESIASI"
BASE COUNT         8327 a         9325 c         8800 g         8219 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctgctcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcactcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccacgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tggaacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc agatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaggccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cagtcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggaact gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgctg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccctc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatac ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctagcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tatgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcactatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtaatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc agctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 ggcgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacaca acatcatatg ttgttatatt catcatgcat tgtcatgttg ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31501 ggcgatatat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31561 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31621 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31681 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31741 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31801 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31861 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31921 tattattgat cggggctatg agggttactg tacggtggag ctggtgacga tgtatatgaa
    31981 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    32041 tgagagatga aaaccctagc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32101 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32161 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32221 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32281 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32341 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32401 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32461 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32521 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32581 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32641 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32701 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32761 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32821 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32881 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32941 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    33001 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33061 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33121 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33181 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag
    33241 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33301 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33361 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33421 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33481 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33541 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33601 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33661 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33721 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggagcgttc
    33781 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33841 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33901 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33961 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    34021 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34081 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34141 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34201 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34261 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34321 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34381 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34441 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34501 atcaggttgt tgctcgtcga cgacctgctt cagtgccaac aggtcattga aatcagcagt
    34561 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34621 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc g
//
LOCUS       DAJTYI010000028        33508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYI010000028 DAJTYI010000000
VERSION     DAJTYI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            535..672
                     /locus_tag="OO564_001336"
     CDS             535..672
                     /locus_tag="OO564_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652517.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO564_001337"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO564_001337"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6652518.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO564_001338"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO564_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6652519.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="OO564_001339"
     CDS             complement(4145..4381)
                     /locus_tag="OO564_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6652520.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO564_001340"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO564_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6652521.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO564_001341"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO564_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6652522.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO564_001342"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO564_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6652523.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO564_001343"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO564_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6652524.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LNANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO564_001344"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO564_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6652525.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO564_001345"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO564_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6652526.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO564_001346"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO564_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6652527.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO564_001347"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO564_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6652528.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="OO564_001348"
     CDS             13569..15185
                     /locus_tag="OO564_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6652529.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="OO564_001349"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="OO564_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6652530.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="OO564_001350"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="OO564_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6652531.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="OO564_001351"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="OO564_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6652532.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="OO564_001352"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="OO564_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6652533.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="OO564_001353"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="OO564_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6652534.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO564_001354"
     CDS             17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO564_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6652535.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="OO564_001355"
     CDS             18432..18959
                     /locus_tag="OO564_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6652536.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /gene="mxiN"
                     /locus_tag="OO564_001356"
     CDS             18901..19596
                     /gene="mxiN"
                     /locus_tag="OO564_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6652537.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /gene="mxiM"
                     /locus_tag="OO564_001357"
     CDS             19589..19996
                     /gene="mxiM"
                     /locus_tag="OO564_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6652538.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="OO564_001358"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="OO564_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6652539.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="OO564_001359"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="OO564_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6652540.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="OO564_001360"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="OO564_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6652541.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="OO564_001361"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="OO564_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6652542.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="OO564_001362"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="OO564_001362"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6652543.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spa15"
                     /locus_tag="OO564_001363"
                     /gene_synonym="spaK"
     CDS             26029..26430
                     /gene="spa15"
                     /locus_tag="OO564_001363"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6652544.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /gene="spaL"
                     /locus_tag="OO564_001364"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26434..27726
                     /gene="spaL"
                     /locus_tag="OO564_001364"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6652545.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spa13"
                     /locus_tag="OO564_001365"
                     /gene_synonym="spaM"
     CDS             27819..28175
                     /gene="spa13"
                     /locus_tag="OO564_001365"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6652546.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="OO564_001366"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="OO564_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6652547.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spa33"
                     /locus_tag="OO564_001367"
                     /gene_synonym="spaO"
     CDS             29016..29897
                     /gene="spa33"
                     /locus_tag="OO564_001367"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6652548.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spa24"
                     /locus_tag="OO564_001368"
                     /gene_synonym="spaP"
     CDS             29902..30552
                     /gene="spa24"
                     /locus_tag="OO564_001368"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6652549.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="OO564_001369"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="OO564_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6652550.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spa29"
                     /locus_tag="OO564_001370"
                     /gene_synonym="spaR"
     CDS             30827..31597
                     /gene="spa29"
                     /locus_tag="OO564_001370"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6652551.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spa40"
                     /locus_tag="OO564_001371"
                     /gene_synonym="spaS"
     CDS             31604..32632
                     /gene="spa40"
                     /locus_tag="OO564_001371"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6652552.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="OO564_001372"
     CDS             32645..32887
                     /locus_tag="OO564_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652553.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10465 a         4873 c         6702 g        11468 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca ttaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc
    33421 cttgcggctg aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt
    33481 atgctgacga aatatcccgg ctcttaaa
//
LOCUS       DAJTYI010000029        32964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYI010000029 DAJTYI010000000
VERSION     DAJTYI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1222)
                     /locus_tag="OO564_001373"
     CDS             complement(<1..1222)
                     /locus_tag="OO564_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6652554.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIA"
     gene            complement(1224..1475)
                     /locus_tag="OO564_001374"
     CDS             complement(1224..1475)
                     /locus_tag="OO564_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6652555.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            1628..2191
                     /locus_tag="OO564_001375"
     CDS             1628..2191
                     /locus_tag="OO564_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652556.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(2467..2889)
                     /locus_tag="OO564_001376"
                     /pseudo
     CDS             complement(2467..2889)
                     /locus_tag="OO564_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(2889..3482)
                     /locus_tag="OO564_001377"
     CDS             complement(2889..3482)
                     /locus_tag="OO564_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6652557.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            3629..4036
                     /locus_tag="OO564_001378"
     CDS             3629..4036
                     /locus_tag="OO564_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6652558.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(4156..5148)
                     /gene="rpoS"
                     /locus_tag="OO564_001379"
     CDS             complement(4156..5148)
                     /gene="rpoS"
                     /locus_tag="OO564_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6652559.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(5211..6350)
                     /gene="nlpD"
                     /locus_tag="OO564_001380"
     CDS             complement(5211..6350)
                     /gene="nlpD"
                     /locus_tag="OO564_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6652560.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(6490..7116)
                     /gene="pcm"
                     /locus_tag="OO564_001381"
     CDS             complement(6490..7116)
                     /gene="pcm"
                     /locus_tag="OO564_001381"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6652561.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(7110..7871)
                     /gene="surE"
                     /locus_tag="OO564_001382"
     CDS             complement(7110..7871)
                     /gene="surE"
                     /locus_tag="OO564_001382"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6652562.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(7852..8901)
                     /gene="truD"
                     /locus_tag="OO564_001383"
     CDS             complement(7852..8901)
                     /gene="truD"
                     /locus_tag="OO564_001383"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6652563.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(8898..9377)
                     /gene="ispF"
                     /locus_tag="OO564_001384"
     CDS             complement(8898..9377)
                     /gene="ispF"
                     /locus_tag="OO564_001384"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6652564.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(9377..10087)
                     /gene="ispD"
                     /locus_tag="OO564_001385"
     CDS             complement(9377..10087)
                     /gene="ispD"
                     /locus_tag="OO564_001385"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6652565.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(10106..10417)
                     /gene="ftsB"
                     /locus_tag="OO564_001386"
     CDS             complement(10106..10417)
                     /gene="ftsB"
                     /locus_tag="OO564_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6652566.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(10611..10934)
                     /locus_tag="OO564_001387"
     CDS             complement(10611..10934)
                     /locus_tag="OO564_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6652567.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(10984..11589)
                     /gene="cysC"
                     /locus_tag="OO564_001388"
     CDS             complement(10984..11589)
                     /gene="cysC"
                     /locus_tag="OO564_001388"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6652568.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(11589..13016)
                     /gene="cysN"
                     /locus_tag="OO564_001389"
     CDS             complement(11589..13016)
                     /gene="cysN"
                     /locus_tag="OO564_001389"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6652569.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSSVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13018..13926)
                     /gene="cysD"
                     /locus_tag="OO564_001390"
     CDS             complement(13018..13926)
                     /gene="cysD"
                     /locus_tag="OO564_001390"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6652570.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14178..15215
                     /gene="iap"
                     /locus_tag="OO564_001391"
     CDS             14178..15215
                     /gene="iap"
                     /locus_tag="OO564_001391"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6652571.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(15361..16452)
                     /locus_tag="OO564_001392"
                     /pseudo
     CDS             complement(15361..16452)
                     /locus_tag="OO564_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(16646..16798)
                     /locus_tag="OO564_001393"
     CDS             complement(16646..16798)
                     /locus_tag="OO564_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6652572.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(17062..17796)
                     /gene="cysH"
                     /locus_tag="OO564_001394"
     CDS             complement(17062..17796)
                     /gene="cysH"
                     /locus_tag="OO564_001394"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6652573.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(17870..19582)
                     /gene="cysI"
                     /locus_tag="OO564_001395"
     CDS             complement(17870..19582)
                     /gene="cysI"
                     /locus_tag="OO564_001395"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6652574.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(19582..21381)
                     /gene="cysJ"
                     /locus_tag="OO564_001396"
     CDS             complement(19582..21381)
                     /gene="cysJ"
                     /locus_tag="OO564_001396"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6652575.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            21700..22062
                     /gene="queD"
                     /locus_tag="OO564_001397"
     CDS             21700..22062
                     /gene="queD"
                     /locus_tag="OO564_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6652576.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            22140..23411
                     /locus_tag="OO564_001398"
     CDS             22140..23411
                     /locus_tag="OO564_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6652577.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            23402..23662
                     /locus_tag="OO564_001399"
     CDS             23402..23662
                     /locus_tag="OO564_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6652578.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            23679..24254
                     /locus_tag="OO564_001400"
     CDS             23679..24254
                     /locus_tag="OO564_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6652579.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(24402..25262)
                     /locus_tag="OO564_001401"
     CDS             complement(24402..25262)
                     /locus_tag="OO564_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6652580.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(25259..26038)
                     /locus_tag="OO564_001402"
     CDS             complement(25259..26038)
                     /locus_tag="OO564_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6652581.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26016..27425)
                     /locus_tag="OO564_001403"
     CDS             complement(26016..27425)
                     /locus_tag="OO564_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652582.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(27447..28901)
                     /locus_tag="OO564_001404"
                     /pseudo
     CDS             complement(27447..28901)
                     /locus_tag="OO564_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(28971..29757)
                     /locus_tag="OO564_001405"
                     /pseudo
     CDS             complement(28971..29757)
                     /locus_tag="OO564_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            30076..31353
                     /locus_tag="OO564_001406"
     CDS             30076..31353
                     /locus_tag="OO564_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6652583.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            31380..32850
                     /locus_tag="OO564_001407"
                     /pseudo
     CDS             31380..32850
                     /locus_tag="OO564_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         7948 a         8729 c         8163 g         8124 t
ORIGIN      
        1 cggcaatacc ggtagggtgt ttaatcccca ggcgtggcag aatacccacg acctctttgt
       61 ccagcatgtc agccgtttca ccggaaccgt tatccgagta atacaggaac ggttttccgt
      121 ggttgcgaat gccgtgctgt atcgcaccgg caacggcgaa cacgttttcg gcaaggtcaa
      181 gactccagcc aacaacaaac cgtgttccac catcgatcac aaacgtgact tccggtgaga
      241 atggacgccc gtggatcgga tgcgcacatt tcagcttcat accgtgaccg tcaccaatcc
      301 agacataatt caccggcata gctgtccagt cgcggcgggt gaatccttca agctgacggt
      361 attcactgcc ggtaacgcgc cctttttgtt tcaccacttc cggcagtttt ttcatggcat
      421 accggacaac atcataggaa ggcatcacct ccagcatgta cggttcgtcc gcatggcgac
      481 gctgccattc tgcgacaaaa tcctcgtagg cttctgacat gggtctgccg ttcacctggc
      541 gatactgcgc cagaaattca ggcagccagc tgatttcttc cggttttatt tcgtcgcgtt
      601 ttcccggtgc cagtaacagc agccgttcgg cggcgttctg cgactggttg tatttcagaa
      661 cccactggta aagtggatcg cggctgatag tacgattagc gccacgtttt gcattggcgt
      721 taactgcggc ctgttgcagg cgctccggca gctcaccttt acgggctttt tcaacaagaa
      781 agttaatggc tttcagtcgc ccgaggttac cggcgctttc cagcttcagc acttccacca
      841 ccagcgcagc tcttgcttca gatacccagc gctggttttc ggtcagcgca ttcaggcgtt
      901 cttccatcag ttgtggagaa ccgcgatatg cttcgattgc ctggacagcg tcaggattgc
      961 gtgtttttct ggtaacagct ttcacttcag aaacattgtt ctctgtagcc accagctgtt
     1021 ccacatagcg ttcacgcagt gcctttcgtg ttatttcagg cagacaatca atgtggtact
     1081 caatggcttt tgtgccttcc cgacggcgac taacgtcacc aagatcacga gaaaaacgct
     1141 gtaacgcctg tcggatgcct tttgtggtgg ctggcattcc tggtacgccg agcaggtcat
     1201 ttacggtaac gtacgactgc atatcacgac gccttcctga taaatccatc gttacgatac
     1261 cggctgggcc agatgatttc tggcggtaca cctatagcgt cagaaattac gcgttcgtat
     1321 tttttgtttg gtgtgcgaac taccgctttc agtgaatcct gagcataacc agcatggagt
     1381 gaaagctccc ggaaggaaga ccacccgtta tctttgagcg ccagctgaat cttcagcgaa
     1441 ggccaatcgc ggtcactatc ttctctttgt ctcatctttc ctttaaccta caaaataacc
     1501 cgcgcggata tccgtatgga taaagattaa gataccaaag attccctata aagggttctt
     1561 tggtgctttt atggtttatt ttttaatttc attatttatc aatgaattag atagaaagga
     1621 accaagaatg agaaaagcac cccaaggttc ttttcctgcc gatggaaaag aaccgattat
     1681 cgaaagaatc tttaggttag tagaacgata cccttcaaga aatgcggctg ctcgggcatg
     1741 ggggatcaat gaaggcactt taaaaaatta ttacaacagg cgggattacg caccgatacc
     1801 acgcccgcat cagttaaaaa agatagcaga aagcgaaaat gttaccttgg aatggctcat
     1861 gacgggagag ggtgatgttg aactaaaaga ttccaaagaa cccaaaaaag agaatgcgga
     1921 taatgttgat gctcaaattc taatgttctt gtcatttctt aaaccacaag aaaaacaaca
     1981 agtagctgat gttcttggga gaaaaggagc ggagcaatta ctcattcttc tggatgagca
     2041 catccaagag ttgcacgctc tagttggagt tcgtagagcc atagcgttaa gccttggtaa
     2101 tttgccagac gaaaaggtta gagagattta cgcgttgtcc gaagctaagg gcgatcactt
     2161 aaatctacct cagaaagcag ccagcgcata acccagtgca agtgcctagc tgtgctaaaa
     2221 ttacattggt tttggcgctt gcacccatca gcgctataat aaacacctga atcgtttttg
     2281 aacggtcttt aaaggctatt gtaagaacct gtgagttatc ttaaatgttt tattgatatc
     2341 gaaccgttta acatttttgg actcgcttta tcttgaaaac cttgagttcc taacttgttg
     2401 attccaatct attcccgtct tttttaatct cttcccggtt atcttgaata aattacctag
     2461 tcatacgaga tagaaaccgc catcgccatc attcaggcga aacagcggca gaatatcgaa
     2521 caggttgatc tcttcgccat cgacggtgtt ctgcgcccag gctggattgg cgcggcgctc
     2581 cggggcaatc gggaagttat cccagcggcg gataaactca tcaatctgct ttttgaccgg
     2641 ggtgtttggt ggcaggccaa gggaaatcgc gtggttctgc caggaaccga tggtattcat
     2701 cgccacgcgg gcatcggtaa agccgcgaat attatcaaac cacagcgcgg gcgcgccgtc
     2761 gccgatacgc ccggtggcgt tagctgctgc tgccagatcc ggctcagcgt tcacttcttc
     2821 gctgattttc agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc
     2881 aaatgccatt attcattctc ctgagaaaaa ttccgggcct gcggcaatcc ttgccagcgc
     2941 ctggcgtggg gatgttcgag gccaaattga tccagcacgc gggctaccac atggtggaca
     3001 atgtcatcta ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg
     3061 cccatgcgtg aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc
     3121 ggcaccagca ccagtttgcg gccttctttg agcacgacgt ccgccgcgcg ccctaccagg
     3181 ccatcggcgt aaccagcgcg gataccggcg agcgttttca tactgcacgg aataacgatc
     3241 atgccgtcag tacgaaaaga accggatgag atgatcgccg cctgatccgc cgggttatgg
     3301 ctgaagtctg cgagggcagc aacatcgcga gcgctgtaag gcgtttctag ttcaatgatg
     3361 gttttcgccc acttcgacat caccagatga gtctcgacat tcggcatctc acgcagcgct
     3421 tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc
     3481 attgcttatt ctcttaattg ttcgtatacg aacattatta ttaacatact ccttcatttc
     3541 cacagcggca aggattgaaa agtaacaaca tcttcttcgt cactaaaaaa ccgctatgat
     3601 agcgacagag aattagcccg gagtttacat ggcgttacga aataaagcgt ttcatcagtt
     3661 acgacagctt tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa
     3721 accacagtac gcggtgatgc gcgccattgc tgataaacct ggtattgaac aggtggcact
     3781 gatagaagca gcagtcagca ccaaagcaac gctagcagaa atgttggcaa gaatggaaaa
     3841 tcgtggctta gtcagacgtg aacatgacgt cgctgacaag cggcgacgct ttgtctggct
     3901 gactgccgaa ggagaaaagc tacttgctgc ggcgatacct attggtgaca gcgtggatga
     3961 ggaatttttg gggcgtttga gtggagaaga gcaggagctg tttatgcagt tggtgcgcaa
     4021 gatgatgagc acatagaatg ctgattgccg ggtaggacgc tgacgtgtct tatccaggcg
     4081 acaaaaacac aagcttccca aacaaaagaa aaaggccagc ctcgcttgag actggccttt
     4141 ctgacagatg cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag
     4201 gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag
     4261 gccaatttca cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg
     4321 acgtgccagt acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg
     4381 cttcatatcg tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat
     4441 gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc
     4501 gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc
     4561 ttccgcactt ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac
     4621 gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc
     4681 ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc
     4741 cgggtcaaac ttctctaccg cgcggatcag ccccaagttg ccctcttcga taaggtccag
     4801 caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact
     4861 ctcgatcatc cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac
     4921 ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc
     4981 gtccaacaca cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc
     5041 actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa
     5101 ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct
     5161 acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc
     5221 ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt
     5281 gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt
     5341 tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt
     5401 ttgatgataa tcagattacc gtagccgcgc agcgcgttac cagcataaac aacgcggcca
     5461 tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc
     5521 ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag
     5581 ataggcgtac tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc
     5641 gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca
     5701 ctctgttcac ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg
     5761 gaattttgtg caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatagca
     5821 tttccgccag tgattggcgt accggaagca ttacccacct gcaaggtctg accaacgttc
     5881 agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta
     5941 atccaggcga tatagaaaag tgtgtcgcct tttttcacgg tataggtact gccgctataa
     6001 ctgcctttcg gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt
     6061 accggctgct gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt
     6121 tgtggctgct gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt ccctattttc
     6181 ggcggcggcg taatcaacat accagaatta gtatttgcag gcgcattgcc attaacggag
     6241 ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt
     6301 gacaaagccg caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca
     6361 ggaaaaattg gttaataacc agtgacataa ttaccgtgca aggcacccta ctgaacactg
     6421 gaaaagatgt tcacgatacg ctgacctacg gcaaaataac caggaaaaat ccaggtattt
     6481 cctcacgttt taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt
     6541 atcgataata aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc
     6601 tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc
     6661 cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca
     6721 tccatcacca tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgtgc
     6781 ctgccactgc aagcctttaa tccgttcaac cgagcaaaca tgctggacaa gatgcgccag
     6841 gattgccgtt tgatatcccg aaccagtgcc aatttccagc acccgcgact gcggcgtcag
     6901 ctcgagtaat tcggtcattc gcgccaccat atacggctgc gaaattgtct gcccctgacc
     6961 tatcggcaag gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc
     7021 acgcggcacg gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa
     7081 ttgatccaga agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt
     7141 aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg
     7201 atggagacat agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta
     7261 ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc
     7321 tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag
     7381 ggtaaatccg gaacgttaat attaagaata cgtccggtgc gcagcggctc tttacacagt
     7441 gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc
     7501 gagacggcca gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg
     7561 gaataaataa catcatcccc cagattcggc ccggcgttaa ttccggacac aacaatgtcc
     7621 gggcgcggac gcatcagagc attcacgcca agatagacac aatcggtcgg ggttcccatt
     7681 tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga
     7741 gaatttgaag cgctgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca
     7801 cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc
     7861 aatatgcgca taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact
     7921 acccgccgga agccagaaac ggatctctac ggtgacgtca tcccaccagt tccagcttaa
     7981 ttgttgcgga tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa
     8041 agcttgtaat tcagtttctg cggcgacagc tgcttgttcg aatgccagcg cttcacgctg
     8101 agttccccat tcgccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttgac
     8161 gcgacgctgt aattccgcca gttcttcggt ggttgctaca aaccagctac cacgtccggc
     8221 taattgtagc gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc
     8281 aacaatctga ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg
     8341 atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc
     8401 aatcccaaaa cgttgggcac cgaagtagtt cggtacacct tttgcgcaaa tatcgatcag
     8461 acgttgttca acgtcatcgc gattgctcac ttcgcgcaga accagggtaa aggcgttacc
     8521 tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc
     8581 ttccagttga aaggcgctca gatcgggcat ttccttgccc ggcacgcgag cacataacca
     8641 ctgttccgtt acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggaatgaat
     8701 tttcaggaat ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat
     8761 tctaaccaga atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac
     8821 aaagtcttcc ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt
     8881 gagattatca aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca
     8941 atcccttccc cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca
     9001 tcatccatat ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc
     9061 aacatcttcg gtgcctgagc gatgatagtg acatcgacgt tgccaagggt ataacccttc
     9121 gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc
     9181 ggatcggtat ccgggaacag tttgccgata tcccccagcg ccgccgcgcc aagcaatgca
     9241 tcggtcaacg catgaagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa
     9301 ggaatgcgta cgccaccaat gataattggg ccttcaccgc caaaggcgtg tacgtcaaaa
     9361 ccgtgtccaa ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg
     9421 ccagtgccaa atcttccggg cgcgtgactt taatgttatc cgcgcggcct tcgaccaact
     9481 gcggatgaaa tccgcaatat tccagcgccg aggcttcgtc ggtaatggta gcgccttcat
     9541 ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg
     9601 cgtgccataa gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac
     9661 gtttcatagt atcgcgcact ggtgcggcga ggatccctcc cgtgcggctg gtttcgctca
     9721 acgccaacaa tcgcgcgagg tcatcctgat gcaaacaagg acgagcggcg tcatgcacca
     9781 atacccactg cacatcgcca gcggctttca gacctgccag cacggaatcg gcacgctcat
     9841 caccgccatc tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc
     9901 tatcgccagg acttatggca atgacgacac gtgtcacccg gggatgcgcc agcagcgcat
     9961 gcaccgagtg ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg
    10021 tttgcattcg acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg
    10081 ttgccatgtt aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct
    10141 tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat
    10201 tacgcgcacg ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa
    10261 gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg cagtgccgcc acatcatcat
    10321 tgacgcgggt atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga
    10381 ccagaatagc cagcaacagc agcgttagtt tacccatcct gccccctgaa aaactgcatc
    10441 atcatcccat gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg
    10501 ggcaaatgta ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc
    10561 attattagtt acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt
    10621 agccacataa acagtgcgcc aaacataatg cctactgtca ccagggtgaa aacaatactg
    10681 tagcgtagct ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacaggc
    10741 atcagtgcca gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa
    10801 atcaacatcg gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc
    10861 agtgaccatg tagtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta
    10921 tggctattac gcatatttga tcctgttact ttgacgaacc gggcattgaa acccggtggt
    10981 gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt
    11041 tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggctctt cgtaaacgga
    11101 atctattccc gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agccttttgg
    11161 atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac acttcaataa agcgcccttc
    11221 tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag
    11281 caccaccagt ccggctgcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc
    11341 tttacgatcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattgtcgcc
    11401 atccagcaga tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac
    11461 cgttgattta ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg
    11521 gtgtagttcg cgttgttgca cagtgaccgg atgactatgc cagacgacgt tttcgtcatg
    11581 cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga
    11641 accagggcat tcaattccag ttcgaatgca ctgaattcag atgacgcagc tgttgcctgg
    11701 ctaactggct cgtgcaccat accggcaccc acggtcacac tgctcaggcg atcgataaaa
    11761 atcagcccac cggtaaccgg attttgctga tagcgatcta acaccagtgg ctcgtcaaaa
    11821 gtgagatcca ccaggccgat gccgttcagc ggcaggtttt caacttcgcg ttgggtaagg
    11881 ttattaatat caacctgata acgaatgcca tcaacacgag cacgcgtctt cttaccggca
    11941 attttgatgt cataactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc
    12001 accgacgcac tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag atcaccacgg
    12061 ctgatatcga tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa gacttcttcg
    12121 cgatcaccat caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact
    12181 tttacacgtt gcccgacttc cacgcgaccg gatgccagca ttccggcgta gccacgaaaa
    12241 tcgagatttg ggcggttaac gtactgcacc gggaagcgca ttggctgggc atccaccact
    12301 cgctggatct ccacggtttc cagcacttcg agcagtgtca gaccgctgta ccacggcata
    12361 ctttcacttt gcgaagccac gttgtcacct tccagtgcgg agagcggcac aaagcggata
    12421 tccagattac ccggcagctg cccggcaaag gtcagataat cttcacgaat acgggtgaac
    12481 gtctcttcac tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc
    12541 cccaacagtg tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca
    12601 tcaatcagta agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgagtgtac
    12661 tgctcgtgcc ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg
    12721 taggccacat caatagtgat gccctgttcg cgctcagctt gcaggccgtc caccagcaga
    12781 gccagatcca gcttttcgcc ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag
    12841 agctgatctt cgtagatttg gcgggtatcg tgcagcagac gaccaatcag ggtacttttg
    12901 ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc
    12961 atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtacggt gttcatctta
    13021 aaaatacccc tgacgttttt tcagctccat ggatcccgcc tggtcgcggt caatcacgcg
    13081 accctgacgt tcactggtgg tggaaaccag catctcttcg atgatttccg gcagtgtttg
    13141 tgcatttgac tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat
    13201 ccgttttttg atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa
    13261 cataccgtcg cgttccagaa ccggacgttc cgcagcgaga tacagcggca caatgtcgat
    13321 attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaagac
    13381 gcggatgctt tcgcctttgt taatttgccc gttgtagttg tgccacagct ccgggcgctg
    13441 atttttcgga tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg
    13501 ggatttctct tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag
    13561 cgcctgtttc aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa
    13621 tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta
    13681 ggctttcgcc gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac
    13741 atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat
    13801 gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac
    13861 ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtttttg
    13921 atccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag
    13981 ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac
    14041 gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag
    14101 atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg
    14161 gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg
    14221 gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata
    14281 cccaggcacg acatattgct actttctttc cgggacgcat gaccggaact ccagcagaaa
    14341 tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc
    14401 ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagaac tggcataacg
    14461 tgacaggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcatcatta
    14521 tggcgcatct ggatacttac gccccgctga gcgatgctga cgcagatgcc aatctcggcg
    14581 gactgacgtt acaaggaata gatgataacg ccgcaggttt aggtgtcatg ctggaactgg
    14641 cagaacgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagcggcg
    14701 aagaggaagg aaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga
    14761 aaaatacgct gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca
    14821 acagcggtgt aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa
    14881 ttgcacgcag tcacggaata gccgcaacga ccaatccggg tttgaataaa aattatccga
    14941 aaggcactgg gtgttgtaat gacgcagaaa tattcgacaa agcgggcatt gctgtacttt
    15001 cggtggaagc gactaactgg aatcttggaa ataaggatgg ttatcagcaa cgcgcaaaaa
    15061 cacctgcctt cccggcggga aatagctggc atgacgtaag actggataat caacaacata
    15121 ttgataaggc acttccagga agaatagaac gtcgctgccg tgacgttatg cggataatgc
    15181 taccgttggt gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag
    15241 ttctaccgca ggggcgggga attctaagtg atacccatca tcgcatccag tgcgtccggt
    15301 ttacttccgg gaaaaccgca ccgtattgcg ccggtttatc tccgctggag cggggaacac
    15361 ctctggcgaa tagaaagcaa gccaggtttt gaccagtcga tcgtacatgg cattggccgt
    15421 tgccattgtt ggcaggccaa aaaacagccc ctgagcgtta cccgcagcca tcaacctgtg
    15481 cgccaggata agcgccgctt ctgttttacc tgcgccggtc acatcttcca gaataaacaa
    15541 ctgtggtcct ggctgattga tatccagatc cagcaccttt tgctgtaacg gtgtcgtatt
    15601 ctcaataaaa ggaaacagcg tattaattcc ggtgaaaggc gcggtttctg cttgtggcgg
    15661 aaagacggtt aatgcgtttt gagcctgaac taaagccttt tgccagtaat cttcaatatc
    15721 tattgcctgt gcgacgcgtg gaaaaaatcg cgttgacgac cctgtccagt ctgcaagtac
    15781 gacggttgca gagatatacc aggaaagttg ttttataagt tcaacgccct cgtcatcatc
    15841 ccagaatgtg gggatctcta ttcgcggaaa cagtggcttg atttcaagga gaaaatctcg
    15901 tgctgcagct ttgtcttcag gcagaaaatt atccaattca tccatacgat cagggggccg
    15961 accatgatgc cctgtcgtca cgggcatcca catttctatc actcgcctaa gtttgcgcgg
    16021 agagagcgaa gatgaaggaa gtaactcctg acattcactt aaataatgat tccacaacca
    16081 gtagcccagc gttgaatgag agattttttc gtaatttttt ctggcacctt cctgaatctt
    16141 gaattcaggg ggcaggtaaa gttgttgaaa agagcgggca aattttccaa tatcgtgcca
    16201 gcacagcaac caggcgaaaa attgagctgc ctgttccttg tcagaaatcc ctaattgacg
    16261 aaaataatca gccagcccga agcaatttct tttaaccatt aaatagccca ttgcggccac
    16321 atccagcaaa tgccagcaaa gaaggtgata gtcgtcgcta ccctctttct cgccacgtcg
    16381 gttttttccc cagaaatcaa agaaagtcac aatattttta tccttcagta aacttaaagg
    16441 atatttacgc atattttaaa aactatctgt gatatatatc agccaataaa acatttacat
    16501 tatgaataaa ttaatgattt tcatttgaaa ttcataataa taaatagaga ctatatattc
    16561 acataaaaaa tataaatttt aaaaaagggt gcaggagacc aaaccatcac aattcagtac
    16621 gcggggtttt acccccgcgt ggtggctact tcttcgattc gtaagcgaga actgccttaa
    16681 actccatacc acgttctctg atactcaact cacataacga gtcacctacc atcaacgtga
    16741 atcccagtac tgtacaacac agtacgatga ttgccacaag ggcatatttt gtcagcatgt
    16801 cttgcctccc tgcaaagaag agactaacat ccgaattgct acgttcgaac gcgagcctca
    16861 ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc
    16921 cagacagcct caagcaccca acgccattct atacctgtta attttccctg cgatgttcac
    16981 ctttaaatgc cggaatgctg ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc
    17041 gtttaattta tccggcaata tttacccttc gtgtaatcca cattcccttt ttaagccaaa
    17101 gaaacgcgtt tcttcttcca ccatgccggg ttcccattta cgggttgtat gggtatcgcc
    17161 caccgataaa tatccttcgt cccataatgg gtgatatttc aggccatgtt tttgcaggta
    17221 ctgataaata gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg
    17281 aattgccagc accggtaaat tggcacggct gccggattgt tcacgccgca ggccagcaaa
    17341 ccacgtttgc gcattcagtt ctttcagcgc ccggttcatc ggttcgactt tgttgatgtc
    17401 attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc gtgcttcctg
    17461 ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt tgtccgttaa
    17521 ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga ggatcaccgg
    17581 aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa tgccaaagct
    17641 ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc ggccttcagc
    17701 gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat ctaccttcgg
    17761 cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt ttgccttgcc
    17821 tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt taatcccaca
    17881 aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga
    17941 agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca
    18001 ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt
    18061 tgccgccaag atgcaggttg tagcgacccg gtgctttacc caccaggccc acttccgcca
    18121 gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc
    18181 tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct
    18241 ccgcttccgc catcgccagc gggcaagtcg ggaatgacac gcaggccatc gagttttcac
    18301 gctgcggcgt gacggcattc attaacccgc tctctttggc aattttctcg atcttcgctt
    18361 tctcgctttc cggcacaccg gcgatgatca gattctggtt cgccgtaatg cggaaatcac
    18421 ctttgtggat cttcgcgatc tccagcaggc cagttttcag cggacgcccc ggataatcaa
    18481 ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc
    18541 agccaatacg atcgccgcgt cctgtaaatt catacgggcg gatcggttca aatttgatcc
    18601 ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agtgtgtatt
    18661 ttgttttggc atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg
    18721 cttccgccac cgctagcgta tgctctagcg gcagatagcc aaactcactc gccgtgcggg
    18781 cgtaggtttt cttgttaccg tgttcgatag aaagcccacc gcccaccagc aggttgaagc
    18841 ccaccagctt gccgttttcc gcgatcgcca cgaagttcat gtcgttggcg tgcagatcaa
    18901 tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac
    18961 caaggatcgg ttcttcatcg gtggtggcga ctttttcctg atcgagccag atctccgcat
    19021 acgcgcgggt acgcggcagc agatgttcag aaatcttctt cgcccactcg tacgcttctg
    19081 cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag
    19141 cagtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct
    19201 ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg ctgccataga
    19261 tggtgttttc accggcaaat ttatcgatcg cctgccactg tttggtggtg ataactccac
    19321 ccggcagacg gcagcgcaac agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt
    19381 cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt
    19441 tatcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca
    19501 ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag
    19561 gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag
    19621 ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc
    19681 cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg
    19741 ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tatcttgtac
    19801 gtaaactttt tctttttggt cgcgcgacca ggcgagatcg atccgcgtca gcacaccctc
    19861 tttgacgtag cgctgccact ccacctgata caggaagtct tccgtaaaat gcggattgcc
    19921 aaagaacagc cagtttttac ctggcgcttc gtcagcggcg cgttgctgca taaaggcgcg
    19981 gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttcca ggttagcggg
    20041 caggcggaag ttatcgttat gttcgataaa tacgcggact tcgccctctt cttccacgcg
    20101 gtcagcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac
    20161 cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg caatggaata
    20221 gagacgcggg gtcagcgggc gcagcagatt aattagcgct tcggcatcaa gctgtgccgg
    20281 ggagaaacgc accatgtcaa caatcagcgt cgtcgcggcg taatgctgta acttcgcttt
    20341 atcgcccacc agcggcagca gtgtttcact gcgggtaagc gtggcgtaat tctcaacaat
    20401 gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt
    20461 tttgccctcg acagtgacag gttcatcgcc tgtcagccac agcagttcga caagttcttt
    20521 caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct ggtaacgcag
    20581 gcccgagtcg cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt tacgcccagt
    20641 aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt acgggctggt
    20701 gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg
    20761 cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac
    20821 atcagaatcg acacggtcga gcaggcgttc accacccagt tccgccagct tgctgtcaaa
    20881 atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa
    20941 cgcggtgttt tccagctttg gcgctttttt ggagaacagg aacttatgca gcgcgacggc
    21001 ttcttccggc ggttcccctt ccccttgcgt tgacgtcact acgatgagca gtttttcgct
    21061 ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgctgc
    21121 taataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt gcgaagcaga
    21181 gataatcgtt atacccggca tttctgcggc gggcgctggc gtcactgcaa gcgcaacagg
    21241 ctgctggttg agcacgcccc agaaatagcc agaaacccag gcaagctggg taggcgttaa
    21301 atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga
    21361 aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc
    21421 ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta
    21481 aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta
    21541 acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa tcattaagat
    21601 aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg
    21661 tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa
    21721 gatttcacct tcgaagccgc tcaccgctta ccacatgtcc cggaagggca taaatgtggt
    21781 cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat
    21841 acgggctgga ttatcgattt cgctgaactg aaagcggcgt ttaagccaac ttatgagcgc
    21901 ctagatcacc attatctcaa tgatattcca ggtctggaaa acccaaccag cgaagtttta
    21961 gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta
    22021 aaagaaacct gcaccgcagg gtgtatctat cgcggcgaat gataagagtg tgtcggcggt
    22081 caatttccct ttagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca
    22141 tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt
    22201 tacgctgcgc gcgagcggat ttatccgttt tgttactcga acgcgctgaa atccccggca
    22261 gcaaaaatct ttccggcagg cggttatata cccatgcact cgcggaactc ctcccgcaat
    22321 ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc ctgttaacgc
    22381 cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac
    22441 gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc
    22501 ccggagcgac ggtggatgca ctgtatgaag aaaacggcag agtctgtggc gttatttgtg
    22561 gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg
    22621 ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag
    22681 aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag
    22741 gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc gcatttcttt
    22801 atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa
    22861 gtcgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca gtgcgcacgc
    22921 ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc
    22981 acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca
    23041 gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact ggcgcacagg
    23101 cggcggcaca aacgctaata agcgcctgcc agcaccgcga gccgcaaaat ctgtttgcgc
    23161 tttatcatca caacgtagag cgcagcctgc tatgggatgt tctacaacgt tatcagcatg
    23221 ttccggcgct tttgcaacgc cctggctggt atcgggcgtg gcctgcgtta atgcaggata
    23281 tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc cagttattct
    23341 ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg cgatgttatc aggagtctgc
    23401 gatgtctgta gcccgtaatc tctggcgcgt tgctgatgcg ccgcacattg ttccggctga
    23461 ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggtag gtcttttttc
    23521 tctcacacag gaaggtggct tacgtattga ctatcgcggc tgcctggagt gtggcacctg
    23581 ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt ccggattcgg
    23641 catcacctac cgctttggat aaataataag gataatttat gcccctctta cacctgctcc
    23701 gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg gccatcgatt
    23761 ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg caccatcagt aatatcgtta
    23821 aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt
    23881 caaataaaga agtggtgatt cagtttttga aactggtgac cgaggcggat ggaattatca
    23941 gcaccaaagc atcgatgcta aaagcggcaa gagcggaagt ttttttctgt attcatcgcc
    24001 tgtttattgt tgattcgatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc
    24061 cggattgtat tgagatcctg ccaggctgca tgcccaaagt gctgggctgg gtgacggaaa
    24121 aaatccgcca accgctgatt gctggcgggc tggtatgcga tgaagaagat gcgcgtaatg
    24181 cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggagtctgg acgttagcga
    24241 aaaaattgct ttgacgggat aatcgtaacc aattgaattt gttttgattt gttggccgca
    24301 cgccacatcc gacattcagc gcctgatgcg acgcttaacg cgtcttatca ggcctacagg
    24361 tccccagtcc agaaccgtag gcaggatcat ccggcagcac gttattggta ttctgcgtga
    24421 atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgtc aatatctgcc
    24481 tgtgaaaata ccgctgcgct ggcgtcatga ttaatcgcca ccataaactt gctattgcgt
    24541 acgcccgcca ttaacgctgc tgcgccggaa gcgccaacaa caatgcacac ctcaggtgcc
    24601 agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc
    24661 gcccgactgt agcccacttc ggttcccagc ttttctgcca gcataacgat ttcctggtta
    24721 tctgcctctc cgccctgccc caccaccagc acccgtctgg cctcagctaa tgggtcgcgg
    24781 ctaacatttt tcagatcctc tgtgctaacc agccagtcgg gaagcgcacc tgggacaatg
    24841 ttcagttgct gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc
    24901 agggaaaggc ataacgggcg tttttctgtt tgcagcgtcg ccgttaacgc atttccccag
    24961 tgggatttgc gcatgcttac ggttggaata tctaacgaag tcacctgaca gatgcttgca
    25021 ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc cggcggaaag
    25081 agaacaacgt ccgcaggcgt tcgctgccac tgttccacca tcgcatccag cacctgttcc
    25141 gccactacgg gctgaggttc tatttgccag tgcgccagcg tacaaccgct gaaatcctgc
    25201 gccgccagcc agctcgctat tgcggcattc tcctgattta ttgtcacaat tgcgatattc
    25261 atggctgcat cctttggcgt aaatagtcct gccatagctt ttgagctttt tctgctaccg
    25321 tttgcccatc aatcagcgtc gctccgcatc gttgctctgc tcttgccagt tgcagacact
    25381 gcatcgctgg catttcggca gcaacagttt tacggataat ttctgctttc cccgcagcca
    25441 ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca
    25501 ccgcaggcaa gcgaacccgg caacagcgca acccatgttc agtacgctgt tcgagggtga
    25561 taaacagcgc gtcgagagtg aaacgctcca ccagggtgaa gcagggccag cccagcattt
    25621 ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta
    25681 tcagatccag cggattctga cgctgccatt cggcaatatg gcgagcgaca aattccggcg
    25741 caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga
    25801 gatagcgcag ccagtgcaac gcccgttcat cccccatgct caacgcggtt aaagacatcg
    25861 gcgtaccgtt ttttctctgc gctaacagca gcgcggcagc ggcctgttca tcagcaccga
    25921 gtaaacttcg cagtagcgaa acatccggtc cgctattacc ctgagccgcc gcctgccact
    25981 ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc
    26041 gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg tgcccatagc
    26101 caggtcacaa caaaaccaac caacaacact gtcgccagga gcaataaggt gacttgcatt
    26161 ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc aagtcgactc
    26221 atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc
    26281 aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac aaaaagcagc
    26341 agcgttaatg aactgccgga aggcaaacag gccattacta ccagcgttgc cgccagcagc
    26401 aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac taatcccagc
    26461 agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc atcttccaga
    26521 ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg gattacgagg
    26581 cagacaaaga agatgctgtt aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt
    26641 ttgatgtgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg gggaccaaaa
    26701 tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcacagtag ccaacgtggc
    26761 gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc tgccgatgcc
    26821 agcaaccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc aatacttgcc
    26881 aacacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc gcgatggcgg
    26941 cgcggggaaa attcagccag caaggtgtga cctactgagt aatcgcctcc cagaccaatg
    27001 ccgatcaaaa tgcgcagccc aataagatgc tctggcgtgg tggcaaaaaa ttgcaagaac
    27061 gaagcgagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc aatatggtcg
    27121 gagatccacc caagaaccag gctgccaaga aacaaaccaa ggagcgccga gccgccgctc
    27181 attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gttgaataat ggcgtaacca
    27241 atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc agcaattcga
    27301 cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt catctttacc
    27361 ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag gcaacgcccc
    27421 gtcatcgcgg aacgctgcct gatacattat ttttcaatcg gatagatagt acccgtattc
    27481 ataatgccgt taggatcgaa ctgctttttc agcccttcca gcaacgccca cgcgctgccg
    27541 tgttccagct tgctccagtg aacgcgatgt ttaccaatgc catggtggtg caccatcgaa
    27601 ccgccgaggc gaatggtttc ttcgcagatg attttgttga gcggattgtg gtacttgtcg
    27661 atttcctctt ccggcttaca gtcaacaacg ttgtagtcat agacgaagta catgttggtg
    27721 ccgttctgat agctatgaga ggagtgaccg cccagcatgg tgatgtcgtc ggcgtgcggg
    27781 aactcagtac gaatacggtt aataacgctt tcgtagattt cgtggatgca gctccagcag
    27841 ccggacactt cggtggtaaa gcccatgttg ccggttttga ggatctgcac acgttcggca
    27901 gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcaatcag tttgctgtcc
    27961 acgcgctggc actgcgggta gcgggcaacg atttccgcaa tcccttcgcc cgtcgccttc
    28021 gcaatgcgag ggttaccttc agccataaag atcagcaccc attttccgtc ggcaaaatgg
    28081 gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg acgataccct
    28141 tccaccatga tttcacgcag gatgttgaag ccggttttca tgtcttccag gatatagccg
    28201 tagaagaggt tgttttccgg ggtgtattta aagattttca ctgttacttc agtgatatag
    28261 cacaatgcac cttcgttgcc gatgatgatg tgacgaatgt ccgggccagc cgcgcggcgt
    28321 ggcacgtttt taatgcgtgt gacggtgcca tctgccaaca ctgcctccag accaacgacc
    28381 atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt tgctaccagg
    28441 ccgcccatct gcgccagcgg ctttgactgc ggagaatgcc ccgtggtgta acctttttca
    28501 cgcaacgcgt tttccagcac ttccagcgga acaccacatt gcgccgtcgc ctgcatattc
    28561 tcaatatcaa tattaatgat ttgattcatg gcggagccgt cgagcaccac cgagttttct
    28621 acaacagttt ccagcccacc ttcggtggcg gaagcaccgg tacgcggcac accgttaatt
    28681 ttgtgcgcat tcataaaatt cagcacacgg gatacttact ctgtggaacc gagttttacg
    28741 accgctgccg gaatcggcaa agtataaatg ccatgaatat ccggaaattt acgaaaacgg
    28801 tcaatactgt ttttctttaa tactgtttca tcggtaatta cgcgatctgc accaacaatt
    28861 tcctttagct ggtcgacaat cgctgcgcga gataaagaca tagtaattcc ttcctgataa
    28921 taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatgga ttagcgcact
    28981 aaataaccgc catcgaccac taataaatgc ccgttgacat aattcgatgc ctgacttgcg
    29041 agaaacacgg ctgcgcccat taaatcctga gtatcgcccc aacggtttgc cggaatatga
    29101 tcaagaacgc gctgattggt ttctggatta ctgcgtgtcg ccagcataat atgctgttgc
    29161 ataatagcca ggggcgatac catttacctg aatattatat tgacctagtt catcacaata
    29221 agctttggtg aacccggcaa gagcatgttt agtggcagaa tatgcaggtg accattgtcc
    29281 acctaagtaa gagaacaatg aacagatatt aataattttg ccgcttttct gcgggatcat
    29341 aatttttgcc gcttcatagc ttaactcgaa tgcggcggtc aggttcacat caatcatcgg
    29401 atcccagtcg gcacgaccga agtccagcac cttattcagc ttacaaatac cggcattgtt
    29461 aaccagaata tcgacagtac cgaagcgctc acagcaggca gcgataatct tctgcggtgc
    29521 gccttctgcg gtaatatcca cctgcatgaa gtccacttca acaccctgtt tttcaatcat
    29581 ttcctttgtt tcgccgttat ctttgacgaa gctaggaata aaaatatttg cgccagcttt
    29641 ggccaacgcc attgcaaatg cctgacctaa accgctattc ccgccggtaa caattgctgt
    29701 tttacctttc agggagaaaa aatccattga gaacgcattg agagattcga ttgacatagt
    29761 tagctccaat ttccgtaagg caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt
    29821 tacctttctc attatctctg gtaactggag ataattttta gcataacggc ataactgcta
    29881 atgtgatcgt aatcacagtg tgatattcgt attgtcaaat agttttaaaa agcagaagcg
    29941 tgattactct cacacaacac ctctgctttc catgttattt cttattgcaa ttactagaga
    30001 cctgagaaaa gtaatccacc tgaaagggcg gcttactttt ctcttttttt tgtctattac
    30061 aaatgggaat acgatatgca acacaactca tatcgccgtt ggataaccct cgcgataatt
    30121 agttttagcg gcggcgttag ttttgacctg gcttatttac gttatattta tcaaattccc
    30181 atggcgaaat ttatgggatt cagcaatacc gagataggtt taataatgag tacctttggt
    30241 attgcggcta ttattcttta tgcccccagc ggcgttattg ccgataaatt ttcacaccgc
    30301 aaaatgatta cttccgcgat gatcattacc ggattactgg gtctgttaat ggcaacgtat
    30361 ccaccgctgt gggtaatgct ctgtattcag gtcgcctttg cgataacgac gattttaatg
    30421 ctgtggtcgg tgtcgattaa agccgcctcg ttgcttggcg atcatagcga gcaagggaaa
    30481 attatgggct ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc
    30541 atgtgggtct tttctcgctt tgcaccggat gacagcgcca gcctgaaaac agtcattatc
    30601 atctacagtg tggtttacat cttgttgggg attctgtgct ggttttttgt tagcgataac
    30661 aacaacctgc gcagtgccaa taacgaagaa aaacagtcat tccagcttag cgacatcctt
    30721 gccgttttgc gtatcagcac cacctggtat tgcagcatgg tgatttttgg tgtcttcaca
    30781 atctacgcca ttctgagtta ctccaccaac tatctgaccg aaatgtacgg catgtcgctg
    30841 gtggcagcga gctacatggg gattgtgatc aacaaaatct tccgcgcgct gtgcggccca
    30901 cttggaggca ttatcaccac ctacagtaaa gtgaaatccc ctacccgcgt gattcaaatc
    30961 ctttccatca tcggtttgct ggcgttgaca gccctgctcg tcacgaactc taacccgcaa
    31021 tcggtcgcga tggggattgg cctgatttta ctgctgggat tcacctgtta cgcctcacgc
    31081 gggctgtact gggcctgccc cggcgaagcg agaacaccgt cttacattat gggcaccacg
    31141 gtaggtattt gttcggtgat tggattcctg ccggatgtct tcgtttaccc gattatcggc
    31201 cactggcaag acaccctgcc cgctgcagaa gcctaccgca atatgtggct gatgggcatg
    31261 gcggcgcttg ccatggtgat agtctttacc tttttgctgt tccaaaaaat tcgtactgct
    31321 gatagcgccc ccgcaatggc tagcagcaag taagccagga aaattacagg agaaaaatca
    31381 tgtcgaaaaa atacatcata gggattgatg gcggaagtca gagcacaaaa gtggtgatat
    31441 acgatctgga aggtaatgtg gtttgcgaag ggaaaggctt attgcagccg atgcacacgc
    31501 cagatgccga tactgcagaa catcctgacg acgatttatg ggcatcatta tgttttgccg
    31561 gtcacgattt gatgagtcag tttgccggga ataaagaaga tattgtcggt attggtctgg
    31621 gatccatccg ttgctgccgt gcgttattga aagccgatgg cacggccgtt gattagctgg
    31681 caggatgcac gcgtaacgcg tccctacgaa cacactaatc ctgacgtggc atatgtcacc
    31741 tctttttcgg gttatctgac gcatcgctta accggcgagt ttaaagacaa tatcgccaac
    31801 tattttggtc agtggccggt ggattataag agctgggcat ggagcgaaga tgctgcggta
    31861 atggagaagt ttaatatccc ccgtcatata ctgtttgatg tgcaaatgcc aggcactgtt
    31921 cttgggcata tcacaccaca agccgcgctg gcgacacatt tcccagctgg gctgccggtt
    31981 gtttgtacca ccagtgataa accggtagaa gctctggggg ccggattact ggatgatgaa
    32041 acggcggtga tttcactcgg tacttatatc gcgctgatga tgaacggcaa agccttgccg
    32101 aaagatccgg tggcgtactg gccgattatg tcttctattc cgcaaacatt gctgtatgaa
    32161 ggttacggta ttcgcaaagg tatgtggacg gtgagctggc tgcgcgacat gttaggcgag
    32221 tcgttaattc aggatgccag ggcgcaggat ctttcaccgg aagatttact caacaagaaa
    32281 gcctctagtg tgccacctgg ctgtaatggg ctgatgacgg tgctggattg gctgaccaat
    32341 ccgtgggaac cgtacaaacg cgggattatg atcggctttg attccagcat ggattacgca
    32401 tggatatatc gttcgatact ggaaagcgtg gcgctgacgc tgaagaacaa ttacgacaat
    32461 atgtgtaatg aaatgaatca ctttgcgaag catgtgatca ttactggcgg cggttcgaac
    32521 agcgatctgt ttatgcagat ttttgccgac gtgttcaacc ttccggcacg acgtaacgcc
    32581 attaacggtt gtgcaagcct gggggcagcg attaatacag cggtaggtct ggggctatac
    32641 ccggattacg caacggctgt cgacaaaatg gttcgcgtga aagatatctt tatgcctgtt
    32701 gagagcaatg ccaaacgcta cgacgcgatg aataaaggta ttttcaaaga cctaaccaaa
    32761 catactgatg tgatcctgaa aaaatcgtat gaagtgatgc atggggaatt ggggaatgcg
    32821 gattcgatcc agagctggtc gaatgcgtaa gtgaggaaga ccgggcggga aactgcccgg
    32881 cctgaatata cctgaatggt tatccccgct gacgcgggga actcgaataa ctgaccaacg
    32941 tctggctctc taaatccggt ttat
//
LOCUS       DAJTYI010000030        32597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYI010000030 DAJTYI010000000
VERSION     DAJTYI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            283..1052
                     /locus_tag="OO564_001408"
                     /pseudo
     CDS             283..1052
                     /locus_tag="OO564_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1085..2605)
                     /locus_tag="OO564_001409"
     CDS             complement(1085..2605)
                     /locus_tag="OO564_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6652584.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2696..3403
                     /gene="tonB"
                     /locus_tag="OO564_001410"
     CDS             2696..3403
                     /gene="tonB"
                     /locus_tag="OO564_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6652585.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPVKKV
                     QEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYP
                     ARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGS
                     GIVVNILFKINGTTEIQ"
     gene            complement(3443..3841)
                     /gene="yciA"
                     /locus_tag="OO564_001411"
     CDS             complement(3443..3841)
                     /gene="yciA"
                     /locus_tag="OO564_001411"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6652586.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3946..4485)
                     /locus_tag="OO564_001412"
     CDS             complement(3946..4485)
                     /locus_tag="OO564_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6652587.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4515..5258)
                     /locus_tag="OO564_001413"
     CDS             complement(4515..5258)
                     /locus_tag="OO564_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6652588.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5286..5375)
                     /locus_tag="OO564_001414"
                     /pseudo
     CDS             complement(5286..5375)
                     /locus_tag="OO564_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5615..6253
                     /gene="ompW"
                     /locus_tag="OO564_001415"
     CDS             5615..6253
                     /gene="ompW"
                     /locus_tag="OO564_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6652589.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6313..6819)
                     /locus_tag="OO564_001416"
     CDS             complement(6313..6819)
                     /locus_tag="OO564_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6652590.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6865..7365)
                     /locus_tag="OO564_001417"
     CDS             complement(6865..7365)
                     /locus_tag="OO564_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6652591.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7451..7630)
                     /locus_tag="OO564_001418"
     CDS             complement(7451..7630)
                     /locus_tag="OO564_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6652592.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8011..8817)
                     /gene="trpA"
                     /locus_tag="OO564_001419"
     CDS             complement(8011..8817)
                     /gene="trpA"
                     /locus_tag="OO564_001419"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6652593.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8817..10010)
                     /gene="trpB"
                     /locus_tag="OO564_001420"
     CDS             complement(8817..10010)
                     /gene="trpB"
                     /locus_tag="OO564_001420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6652594.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10022..11383)
                     /gene="trpCF"
                     /locus_tag="OO564_001421"
     CDS             complement(10022..11383)
                     /gene="trpCF"
                     /locus_tag="OO564_001421"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6652595.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11384..12979)
                     /gene="trpD"
                     /locus_tag="OO564_001422"
     CDS             complement(11384..12979)
                     /gene="trpD"
                     /locus_tag="OO564_001422"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6652596.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12979..14541)
                     /gene="trpE"
                     /locus_tag="OO564_001423"
     CDS             complement(12979..14541)
                     /gene="trpE"
                     /locus_tag="OO564_001423"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6652597.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14563..14655)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            14814..15695
                     /gene="yciV"
                     /locus_tag="OO564_001424"
     CDS             14814..15695
                     /gene="yciV"
                     /locus_tag="OO564_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6652598.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15692..16312
                     /locus_tag="OO564_001425"
     CDS             15692..16312
                     /locus_tag="OO564_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6652599.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16413..17285
                     /gene="rluB"
                     /locus_tag="OO564_001426"
     CDS             16413..17285
                     /gene="rluB"
                     /locus_tag="OO564_001426"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6652600.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17325..17915)
                     /gene="cobO"
                     /locus_tag="OO564_001427"
     CDS             complement(17325..17915)
                     /gene="cobO"
                     /locus_tag="OO564_001427"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6652601.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(17912..18670)
                     /locus_tag="OO564_001428"
     CDS             complement(17912..18670)
                     /locus_tag="OO564_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6652602.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            18890..19939
                     /gene="sohB"
                     /locus_tag="OO564_001429"
     CDS             18890..19939
                     /gene="sohB"
                     /locus_tag="OO564_001429"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6652603.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19975..20226)
                     /locus_tag="OO564_001430"
     CDS             complement(19975..20226)
                     /locus_tag="OO564_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6652604.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20606..23203
                     /gene="topA"
                     /locus_tag="OO564_001431"
     CDS             20606..23203
                     /gene="topA"
                     /locus_tag="OO564_001431"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6652605.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23413..24387
                     /gene="cysB"
                     /locus_tag="OO564_001432"
     CDS             23413..24387
                     /gene="cysB"
                     /locus_tag="OO564_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6652606.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24682..24846
                     /locus_tag="OO564_001433"
     CDS             24682..24846
                     /locus_tag="OO564_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6652607.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24849..25016
                     /locus_tag="OO564_001434"
     CDS             24849..25016
                     /locus_tag="OO564_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652608.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25130..25225
                     /locus_tag="OO564_001435"
     CDS             25130..25225
                     /locus_tag="OO564_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652609.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25389..28064
                     /gene="acnA"
                     /locus_tag="OO564_001436"
     CDS             25389..28064
                     /gene="acnA"
                     /locus_tag="OO564_001436"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6652610.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28128..28718)
                     /gene="ribA"
                     /locus_tag="OO564_001437"
     CDS             complement(28128..28718)
                     /gene="ribA"
                     /locus_tag="OO564_001437"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6652611.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            28888..29652
                     /gene="pgpB"
                     /locus_tag="OO564_001438"
     CDS             28888..29652
                     /gene="pgpB"
                     /locus_tag="OO564_001438"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6652612.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29801..30109
                     /gene="lapA"
                     /locus_tag="OO564_001439"
     CDS             29801..30109
                     /gene="lapA"
                     /locus_tag="OO564_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6652613.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30116..31285
                     /gene="lapB"
                     /locus_tag="OO564_001440"
     CDS             30116..31285
                     /gene="lapB"
                     /locus_tag="OO564_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6652614.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31478..32215
                     /gene="pyrF"
                     /locus_tag="OO564_001441"
     CDS             31478..32215
                     /gene="pyrF"
                     /locus_tag="OO564_001441"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6652615.1"
                     /translation="MTLAASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32215..32541
                     /gene="yciH"
                     /locus_tag="OO564_001442"
     CDS             32215..32541
                     /gene="yciH"
                     /locus_tag="OO564_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6652616.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         8235 a         7873 c         8324 g         8165 t
ORIGIN      
        1 cacaactcag acggtacttg atggaaaggt tattgatgaa agaaccacat ctcatattaa
       61 tcgtatgcag gaagatacct tgattagctc agcttaccca gaggaataaa caatgttaga
      121 actactaata tttattgcca cacatggcaa tctattaatc tttatctagt atgacgattc
      181 aactatgaat agtcgaatgc tacgtgattt cttctgagca gataccaaca tcaaaaatta
      241 gatttcacgt agctcacgct taacagaata ggaataacaa caatgtttga cacaaagcaa
      301 attagatact caggtaaaga tggtatctat tttcacctta gagataatgt agaatctttt
      361 cttgccttaa cagctactga acagatggac tatgactcac cgatgatggt gacaaccagg
      421 ctatgcaatc ggggcaaact atcactgata atgttcagag agcacctaag accatctcta
      481 ttagcggtgt tgtagtggtt gattcgacgg gttcagttct attaaccaga caggggcaac
      541 ttgtagagac ctttatttct acggttgaac aatggcgaga ccagaagcaa atcatcacta
      601 ttgtttgtaa agatggaata tctatcactg atagtatcat tactcagttt aaagcgagta
      661 aagagcatac cattgctaat ggtcttcgta ttcagatgac cttccaaggt gtagaatttc
      721 gttcaatcgt agggcaaact gatatttcag cagctactgg caagaccgct accacgaatg
      781 atggtggtgc taccagtaaa aagaacactg gcaacactac aacgagtctt ggaagcccta
      841 tgctgacctg taaagagctt tttagctatt cggcaagtga gttatcagat gaagcgctaa
      901 aagcccgtgt aacgtgctct aagagcgtta gcgttaaaaa tggtgagagc accttcacag
      961 cctcagctaa tgagcgagct tcaaaagtgc ttaagtcggg taatgcttta cggaagtact
     1021 ctgtgaatcc gaataagaag ggcacttatt gagacataaa aaggggcttc atagcccctg
     1081 aaaattacag ttgataagca tcaatatcca gtagctcttt atcagcaatt tcaaagattg
     1141 gtgacccatc atttagcggt agcgggtctg taggtatggt gatttcagtg tcgttctgta
     1201 gcgttatagt tactgaaccc ttacccaatg taagcccttt tagatgtttg gacaaaatta
     1261 attgacactc gacacgaccc gctttagttg tcaggtcaat atctttaagc tggggtagtt
     1321 ccttatcagt gagtgaagcc ttacgcttca tagtatcaat cgtggtctgt agttctgtgc
     1381 gcttctggtt aagttcctta agctgaattt ttagttcatc aatatcatcc atctcaagaa
     1441 gtagggcggt agctttcttg attctggctg tgacaatttc aacctcatgc tgtaacgctg
     1501 caatcttcct ctctgattct ccatcattcc ccacgtcaat catccccaga aaattatcaa
     1561 tcatccatcg gtcaaacggt ttacgcttca acggtggaac atcacagcga ccgtctaagt
     1621 ggttattgca cctgtagacc ccagcatact tattctttgt tccggtagga tggacactag
     1681 ccccacactg acacctaaac agacctttga agatgttaat cgttagtggg ttgtcacttg
     1741 caggtgtacg gcctttacga ttcttatcaa gaatctcttg agctttattg aacgttgcag
     1801 catctatagc ggcttcgtag tagttcggta tagcggggcg gtcattgcgc tgtgatgctg
     1861 gtagtgtacc tatcgttgtc tctgacctta agatagtacg cacagaatga acgctaaatc
     1921 cattagcatc tttacgtcgt gagaatgaac taatgttatt agcctgtagg tgacgtgcaa
     1981 tgttatgcag actttcacca tctagcagca gcttgaaaat caagcgtaca gtttcagcac
     2041 gttcattgag tttaaaagtc tcagggtcaa tccatccggg aacattcctt gttaaacgtt
     2101 gttcaccagc aagggcaagc tttgcacgtt gttcccattt ctgactaaca cggaaagact
     2161 tctgtttact ctcctgatgc gctatgtagc ttttaacccg ctgaatgatt gagattccta
     2221 aatcatcgga tagagcatcc cttgtgtaga ttagcccatc ctgtagagtg tgaatctcga
     2281 taccatgagc aataatgcga ttcatcaagg cttctgtttc aagatgttca aggcggccta
     2341 tgcggtcgat agcttcgact agtaaaattg tcccttctgg aaactcccct gattcaatgc
     2401 gggagagcat atccccaaat tgccctgata agtgtttacc tttgtaaccg gatagacctt
     2461 catcattgaa agatgccact acctgagcat cgctattctt ccttaaccac tgtttgaata
     2521 gctcattttg tcgtctggtt gaatcagcac cttcagcacc ttgtgtggct gatgagaatc
     2581 tcaagtatgt tattacggtt ctcacggtga taaactccca tgcaattgat tatcatttaa
     2641 agatgaactt ataaacgtta tggctaatgc tacagtgccg aatagctaca atacaatgcc
     2701 ccttgattta cctcgccgct tcccctggcc gacgttactt tcggtctgca ttcatggtgc
     2761 tgttgtggcg ggtctgctct atacctcggt acatcaggtt attgaactac ctgcgcctgc
     2821 gcaaccaatt tctgtcacga tggttgcgcc tgctgatctc gaaccgccac aagccgttca
     2881 gccgccaccg gagccggtgg tagagccaga accggaacct gagccgatcc ccgaaccgcc
     2941 aaaagaagca ccggtggtca ttgaaaagcc gaagccaaaa ccgaagccgg tgaaaaaggt
     3001 acaggagcag ccaaaacgcg atgtcaaacc cgtagagtcg cgtccggcat caccgtttga
     3061 aaatacggca cccgcgcgcc ccacatcaag tacggcaacg gctgcaacca gcaagccggt
     3121 taccagtgtg gcttcaggac cacgcgcatt aagccgtaat cagccgcagt atccggcacg
     3181 agcacaggca ttgcgcattg aagggcaggt taaagttaaa tttgacgtca cgccggatgg
     3241 tcgcgtggat aacgtacaaa tcctctcagc caagcctgcg aacatgtttg agcgtgaggt
     3301 gaaaaatgcg atgcgcagat ggcgttatga gccgggtaag ccaggcagtg ggattgtggt
     3361 gaatatcctg tttaaaatta acggcaccac cgaaattcag taagcagaaa gtcaaaagcc
     3421 tccgaccgga ggcttttgac tattactcaa caggtaaggc gcgaggtttt ccttcaggat
     3481 caaccgcgac atacttaaat aatgcttctg tcgctttata gcgttgcccg attggttcag
     3541 acgctacttt tttcacccac acttcaatat taatgctgac cgatgtcgtc cctttctgga
     3601 cacagcgtgc atagcagcac accacatcgc cgaccgcaac cggccgtaag aaagtcattc
     3661 cttcaacccg cacagtcact acgcgaccgt gggcaatttc ttttgccaga atagcgccgc
     3721 caatatccat ttgtgacatt aaccaaccac caaagatgtc accattggca ttggtatcgg
     3781 cgggcatggc taaagtacgt aaaacaagat cgccctgagg gacgttatgt gttgtagaca
     3841 tggtaaaacc gacttaaaag gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga
     3901 gaacagagga gatacagcgc cagccccgaa ggactggcag tctggttagg atttatcttc
     3961 ctgcggcatg tggcggtaga tatagatacc gcttaacaat gtgaagatta aggtaagggc
     4021 ggtcaggcca aagactttaa agttgaccca aatattttgc ggcagccaga atgcgatgta
     4081 gatgtttgcc agaccgcaaa ggataaagaa aacagcccag gccagattta gcttcgacca
     4141 taccggttgc ggcagcgtga gttctttacc cagcatccgc tgaattagcg gctttttcat
     4201 cacccattgg ctgactaaca gggctaccgc aaacagagca taaatgactg taaccttcca
     4261 tttaataaac tcatcattgt ggaagaacag cgtcaagcca ccgaagacga ccaccagaac
     4321 aaaagtgatc agggccatct tctcaacctt acgaaagcga acccagctat agataagcac
     4381 aatcgccgtg gcgacgatca gcgctgcagt agccgcatag atgtcataaa tcttgtaaaa
     4441 cgcgaaaaag acaaccagcg gtaaaaaatc aagaaactgc ttcattttac gattccgtaa
     4501 tcaaatgctt aaggttattg gcgaatcaac atatacaggc ggaacagata gatgagcaat
     4561 atggcagaaa tcaggttgct caaggtgttc gccagtacgg caccaatttc cggggttaat
     4621 gcggcaaaag aagaggcaaa aagcagcaac agtgtttttg ccagcaacca gctcagtact
     4681 gcgggtgcca ccagacgcat attcgcccaa gtcagccgca tactgctacg catcgaggca
     4741 aaaatgccca ttttgtcctg taccagcatc accggtgcca gggccagtaa aatggccatg
     4801 ataattcccg gaacgaccac cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa
     4861 atcagaataa ataacttggg caatatcggc gcactggcac caatagctcg cagcgcacag
     4921 actctctgac ccgcagacac cagctggata attaatatta cgcctccggc gagaatggcg
     4981 ttaccgatta attctgaaaa agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt
     5041 tccggtgaca tattctgaac caggtcgaac aacccactac tgccgctaac gggcacgccg
     5101 tcattgagct gcgcaagctg tgcatcactg ggtgagaaaa catgccctaa caccactgtg
     5161 ataaacgcac atagcaacga taccaacaga atggtcataa attgattacg gaagaaattt
     5221 ccggtgtcac ggtatacgga ctgcgccgtg atagacatgc actctccttg agttttatgc
     5281 aggtgttaat tagcgggcaa ttgtaccctg gttaagcctc gggtggcagc atcaggtctt
     5341 gtatggaaaa gcatatcttt gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa
     5401 ccgcggcaag tggcgatcga gttaatattt gcgtagagaa aatatttaaa aattgattta
     5461 aatcacatta accaggattc tcaatgcaac ttctaaatta atccagatca ataaatggtg
     5521 aattatcata tgtaatgtga tctatgtagg atcatttgtt actccaatgt aggtatattc
     5581 gtcacgtttt tataaccata acgacggagc ggatatgaaa aagttaacag tggcggcttt
     5641 ggcagtaaca actcttcttt ctggcagtgc ctttgcgcat gaagcaggcg aattttttat
     5701 gcgtgcaggt tctgcaaccg tacgtccaac agaaggtgct ggtagtacgt taggaagtct
     5761 gggtggattc agcgtgacca ataacactca actgggcttg acgtttactt atatggcgac
     5821 cgacaacatt ggtgtggaat tactggcagc gacgccgttc cgccataaaa tcggcacccg
     5881 ggcgaccggc gatattgcaa ccgttcacca tctgccgcca acactgatgg cgcagtggta
     5941 ttttggtgat gccagcagca aattccgtcc ttacgttggg gccggtatta actacaccac
     6001 cttctttgat aatggattta acgatcatgg caaagaggcg gggctttccg atctcagtct
     6061 gaaagattcc tggggagctg ccgggcaggt gggggttgat tatctgatta accgtgactg
     6121 gttggttaac atgtcagtgt ggtacatgga tatcgatacc accgccaatt ataagctagg
     6181 cggtgcacag caacacgata gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg
     6241 atatcgtttt taattccgca caaaaacgac cccgtaatat acggggtcaa taaggacatg
     6301 gtataaaggg tattatttct tcgcttctac gcaatcagtt tcagaacgaa ttaaaaattt
     6361 ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc atttgctttt cctcatttaa
     6421 aatcgcttcg atgatcggaa ttgagactgt atcaccggca ttttttgctg ctgctaatag
     6481 tgaggtgtaa caggcgattt caaattgctc gaagacatat ccgctaatag agcctttaac
     6541 tatttcatca gaagggaata taccaccgat tgactgccca agcgcagcca ttttactcat
     6601 ggaatcttta atgactgaac gtgaaatgtc attacgatca agaatagttt ccagttgaac
     6661 aatctggttt ttggtttcac taagatgttg ttcaatacga gcgcgtagtt caggataatt
     6721 atctatacgg ctggccatgg attcaagcat agattcggct tgcttttcca ttgcgtgggc
     6781 gtcacgtaac cagtcatgat aatgttcaat acgattcatg aaaatgtcct ctttataaaa
     6841 tgactaaaag ttaaaattca tatttcaggc tttattttcg gctttcttat ttacgttatt
     6901 aagggccaga tcagtcagtt tgatgtcggt ggccttttct tcttccaggg tttctttcag
     6961 aagcttcgct gctttacggt aacctaattg ttcagccagc gtcgctaatg tcccgtaact
     7021 ggcaatctca taatgctcga ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc
     7081 gtttttctcg gtactttcga tgacctcatt agcttcttca ataagacctt ccattgccac
     7141 acatttcatg cgcttaattt tcagatttga ttctgattcc acaacttgat caattcgttc
     7201 aatctgtcca tgagtttcct cgaggtgcgc atgaaaagcc tgacttaatt tttcatttga
     7261 tgttgctctt gcgagttttg ccagtgcccg gggtaattgt ttttctgcgc tgtaggtatc
     7321 tgaaagcagt tgaataaata catcttcaat ggtcttcata ttcatatttt tctccagtga
     7381 aatcactgcg agccatacgg cccgcagatg caattatctt tatcagctga tattaataat
     7441 taacggtgaa tcaggatttg tctgatttat taccaccgct ttgttgaccg ccttttttac
     7501 ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc gccgctatgc tgaccgcctt
     7561 tacggcctgc gtcggatgcc ttctcacggt cttcggcgaa atttcctgaa ccaccacgat
     7621 gttcggccat gttatttctc ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg
     7681 tcgacactca ttaaaattag tcgctaatga gaattagtca aattaagcgc aacgagaaga
     7741 tagagggaaa atatattttg aggaacattc tggatatatt aacaattacc tgaggaataa
     7801 atgacttaga gaaaattatt gattttactg gtgttatgtt gcgggattaa tttgtttgat
     7861 tatattactg ttgggcggaa aaatgacgta agttgacgtt cgaccggggt aagcgaaacg
     7921 gtaaaaagat aaatattaaa tgaatttagg atttttccgg cttcattaaa gaaagttaaa
     7981 atgccgccag cggaactggc ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg
     8041 tacaaaaact ttcagtgccg ccagcatttt ctctggctca ttaatatgtt gctcgatgat
     8101 tttaacaatg gccgaaccag aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac
     8161 ctgatccggg gcggaaatac caaacccctg caatggaggt gcagcgttgt actctttcag
     8221 cttcgcaacc agatgattga ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc
     8281 tcgtgacagc aaatatgtgt aaccacgacc gtaagaggct atctggcgca gcaggtcgtc
     8341 atcggcattt ggcgggcaga tgaagatagg tgcgacatta tggcgcaacg cggcctggcg
     8401 gaagggcgcg gactcttcaa ctggcacatc ggcaaccagc accgaatcga cgccgacttt
     8461 ctcgcactgg gcataaaact catcaatgcc tttgttaaac accagattgg catacatcaa
     8521 caggccaatg ggaatggtcg ggtgtttctg gcgaatcagt gccagcattt caaaacattg
     8581 tgccggagtc acacctgccg caaaggcgcg cagagtggcg ttttgaatgg tcgggccatc
     8641 cgccagtggg tcggagaagg ggatacctaa ctccagcgcg tcagcaccgg cttcaattag
     8701 cgtatcgata attttcaatg actgctcaat gcccggatca ccgagcgtga cgaaaggaat
     8761 gaatgcgcct tctttgcgct ccttcaactg ggcaaacaga gattcgtagc gttccatcag
     8821 atttcccctc gtgctttcaa aatatcgtga acggtgaaga tgtctttatc gccgcgaccg
     8881 gaaaggttaa ccaccagtag ctgctcttta tccgggtttt cgcgcatcat tttcagcgca
     8941 tgggccaggg cgtgggagga ttccagcgcc gggatgatcc cttcgtgcag gcacagcgtt
     9001 ttgaaggctt caagggcttc atcatcggta atagacacgt aatcagcgcg tccagtgctg
     9061 ttaagatacg cgtgttgtgg gccgacagac gggaaatcca gtccggcgga gatggagtaa
     9121 gattcttcaa tctgcccgtc ttcggtttgc atcatcggcg ctttcatacc gaaataaatg
     9181 cccacgcgac catgttttag cggtgcgccg tgctcgccag tttcgatacc gtgaccacca
     9241 ggctccacac caatcaggcc gacgttggtt tcattgatga aatcagcaaa catgccgatg
     9301 gcattcgaac cgccgccaac acaggcgata acggcatccg gcaggcgacc ttctctttcc
     9361 agaatctgcg ctttggtttc ttcgccaatc atccgctgaa actcacggac aatggtcgga
     9421 taaggatgcg ggccagccgc ggtgcccagc atatagtgcg cggtttcata actgccggac
     9481 cagtcgcgta gcgcctcatt acaggcatct ttcagcgtgg cggaaccgct atgtaccggg
     9541 atcacttccg cacccattaa gcgcatacga aaaacgttag gcgactggcg ttcaacgtct
     9601 ttggcaccca tataaatacg gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc
     9661 acgccatgct gaccggcccc ggtttcggcg atgatttcgg ttttacccat ccgcttcgcc
     9721 agcaacgcct gccccagcac ctggttggtt ttatgtgcgc cgccgtgcag caaatcttca
     9781 cgcttgagat acagcgtggt gttcgtcccg gctgtaatgt tctggcattt ggtcagtgcg
     9841 gttggacgcc cggcatagtt tttcagcagg tcgttgaact gggcttgaaa ttcaggatct
     9901 ttttgcgcgc tgacaaaagc ttcttccagc tggcgcagag caggcatcag gatttgtggc
     9961 acgtacatgc cgccaaactc accaaaatag gggttaagta atgttgtcat tgttcctttc
    10021 cttaatatgc gcgcagcgtc tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg
    10081 gttgcgactc tacagcagaa ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca
    10141 cgcagttatc tgcgcctaag ccccccgcca gcagaacgtt gccaagcgat tgaccattta
    10201 atagtgacca gtcaaaacgt tgcccgcttc caccctggcc gttgtctaaa acatatttat
    10261 caacgtgctg aaactcgcgg gcgggcaggg tttcaccgac gcttaatgct ttccagatgg
    10321 caacatgtgc tggcagagct tcacgcagcg tatcgatata cagctgatct tcattaccat
    10381 gcagttgcac tgccgccagc gataacacct tagctttgtc caccacatcg gcaatatcgt
    10441 gattgcggaa cacgccaaca tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt
    10501 caacgttgac gcaacgcggt gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg
    10561 cgtcataagc tgctttagca tcttgcccac gcgtcaggcc acatacttta ttctcaccca
    10621 gcaacacccg gcgcacggcg gcgtgcaaat cgtcatgggc catcaacgcc gaaccaatca
    10681 gaaaaccgtt agcgaagtgg cttaactcgc gcacctgagc gtaagtattg atgccggatt
    10741 cgctgattac cgttacgttg tgccccagtt tcggcgcaag ctcgcgggta cggttgagat
    10801 caatcgacaa atcacgcaga tcgcggttgt tgatgccaac gacctttgcc cctaatgcaa
    10861 tggcgcgctc cagttcctct tcattactga cttcggtcag cacccccatc tccagactgt
    10921 gagcgacggc tgcaagctgg cgatattggt cgtcatccag tactgaaagc attaataagc
    10981 aggcatcggc ctggtaatag cgcgccagat agatctggta agggtcgata atgaagtctt
    11041 tacataaaat cggctgcggg gcgatttggc tgacgatgag gaggaaatca aagctcccct
    11101 gaaaatattt ctcatcagtc agcaccgaaa ttgccgaagc gtaatgttta taaatggcgg
    11161 caatgcgtgc cggatcgaaa tcatcacgga tcacgccttt tgacggcgac gcttttttac
    11221 actccagaat aaacgccgtg cgtgcacctt gtagcgcatc ataaaaatgt cgtgtgctcg
    11281 gctgaacctc attctgaaaa ctggccagcg gttgctgctg tttgcgggtt tctacccaaa
    11341 tcgccttgtc tgcgacgatt ttcgctaaaa cggtttgcat catttaccct cgtgccgcca
    11401 gcgcggtaac tctgtcgtaa gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat
    11461 tggcttgcag atcttcatgg ccatgcaggc gcattaacat ggcgacgttc gcagcgacgg
    11521 ctgcttcatg ggcggcgtcg cctttacctt gtaacaagcg tgttaaaatg tcacggtttt
    11581 cttcaggtgt tccgcctgcc agttgctcct ggtggtaggg agtcaggcca aaatcttcag
    11641 cggtcaattg atagctctta attttgccgt catgcagctc ggcaacgatt gtcggcgcgt
    11701 gtaatgaaac ttcatccatc ccgccgctgt gcaccaccgc cgcgcgttga taccccagca
    11761 cgcgcaaggt ttcggcaatc ggcagcacca gttccggact ataaacacca attaacgcca
    11821 gcggcggatg cgccgggtta atcaatggcc ccagcacatt gaacagggtg cgggttttca
    11881 gttgctggcg aaccggcatc gcgtggcgga atccggtgtg atatttcggc gcaaagagga
    11941 aacatacgcc taactcgtcc agcgcctggc gcgatttatt ggcgttcata tcaagattaa
    12001 taccgaatgc cgccagcaga tccgacgagc cggatttact ggagacgctg cggttaccgt
    12061 gtttcgccac tttcagccca caggccgcgg cgacaaacgc actggcggta gaaatattga
    12121 tactgttgct gccgtcaccg ccagtaccga cgatatcagc aaacagataa tccgggcgcg
    12181 ggaacggcgc tgcgttttcc agtagcgcgg ttgctgcccc ggcgatctcg ttcgggtgct
    12241 caccacgaat tttcatgctc accagcgccg ccgccagttg ttccggcttc agctcgccac
    12301 gcaccaccgc tgaaaacagc tggtggcttt cttgttggct aagcgtctgc gcctgataca
    12361 gtttttccag aatcggttgc agcgtgttgg ctggctctag tttctgctgc gcccaggcca
    12421 gcgtttgttc cagcaggcga gcgccctggg tggtgagaat ggattccgga tggaactgga
    12481 atccacaaac gcgatccgca tcgtgacgta ctgccatcac catgccatta aaatgggcgt
    12541 tgatggttaa accggccgga atgttactgc caaccagcga gtgataacgc gccaccggca
    12601 gcgggtttgt taatccggca aacatcgcct gaccgtcatg ttcaatgctc gacgctttac
    12661 cgtgaagaat ttcgcccgcc tgaccgacat agcccccgta agcttcgaca attgcctgat
    12721 gtccgaggca aatgccaata atgggcagct tgccacgcaa gcgggtgagg agttccggca
    12781 tacaaccggc ttcgctcggc acaccggggc caggagaaag catcagtacc ggattgctca
    12841 tcgtcgccag gcgttcaatt aaggtctgcg ccggaatatg gttgcggtaa atcaccacgt
    12901 tatgaccatt gctgcgcaac tgatctgcca ggttgtacgt aaaagagtcg atattatcga
    12961 gcagcagaat gtcagccatc agaaagtctc ctgtgcatga tgcgcggtgg caatagcgcg
    13021 cagtacagcg cgggctttgt tacgggtttc gtcggcttcc gactgcggaa cagaatcaag
    13081 gactacacca gcacccgctt gcacggtggc gataccgttt tccaccagcg ccgagcggat
    13141 cacaatgcag gtgtcgagat cgccatgcgc ggtgaaataa cctaccgcgc cgccgtagct
    13201 gccgcggcga cgaccttccg cctcggcaat taactgcata gcgcgtactt tcggcgcacc
    13261 gcttaacgtc cccatattca tacaggcgcg ataagcgtgc agggcgtcaa gatcgtgacg
    13321 cagttcgccg actacgcgag agacgaggtg catcacatag gaataacggt caactttggt
    13381 gagatcggcg acgtagcggc tgccaggggt gcaaatgcgt gccagatcat tacgggcgag
    13441 atcaaccagc atcagatgtt cagacagctc tttatggtca gtacgcattt ccagttcaat
    13501 acggctgtcg agatctctgt ccagtgaacc atcggcgcga cgaccgcgtg ggcgtgttcc
    13561 ggcaatcggg tagatctcaa tctggcggct ggtggcatca tacttgagcg agctttccgg
    13621 cgacgcgcca aatagggtga aatcattatc ctgcataaaa aacatgtacg ggctgggatt
    13681 actctttttc agcacgtaat aggccgccag cggtgacggg cagggcagag agaaacggcg
    13741 agatggcacc acctggaaaa tttctccagc gcgaatcgct ttttgcaaca aacgcactac
    13801 gccaccgaac tcttcatcgc tctgattaca ttcacaacgc atatgcggca cggaaaccac
    13861 tggcagcggc ggcgcggctt cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag
    13921 acgttgtttt tcttcttcat tcggagcaaa caggctggcc tgaatacggg tgcttttttt
    13981 ctgatggtca atcaccatca gcgtttcagc gagataaaaa cagaaatcag ggcagttatt
    14041 ttccgctgac agttgcggta aatcttcaaa tcccgccaca aggtcataag agaacaggcc
    14101 gccgaagaac attgcttctc gttcttcctt cggtacattc aacagattct gcaataaacg
    14161 gaaagcgtca aaaaccgaaa gggagcataa gcgggcgtct tcatccagca gtggactgac
    14221 aggggggaag cgcagcacac ggcagtttgg tgattgttca ctttccacac ccgcaggcag
    14281 ggcgttatcc agtagtgcca gcagggcttc gccattgccg gaaagtgcct gaattgtgac
    14341 ggtgtcacct aatgctgtaa tgtgcagcgc attgtctatc agcagcaggc tttttaaatc
    14401 atctttgctg tcgatatctg cggattccag cagcagcgtt gccggacgat tcccacacaa
    14461 ctggtgaaaa agcgcagtcg ggttgtcgcg ataagcgcct tcgcaggtta gcagttcgag
    14521 agtcggtttt tgtgtttgca ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat
    14581 taggcgggct gggtatctga ttgctttacg catggtgaat acactgcccg tttcaggaag
    14641 tggccaccaa cctttcagta cgaaaattgc tttcattgtc gataccattt ttacgtgaac
    14701 ttgcgtacta gtcaactggt tcgatgatta attgtcaaca gctcatttca gaatatttgc
    14761 cagacccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
    14821 acacgaatta tgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
    14881 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
    14941 ccgaccatga caccacagct gcaatcgcgc cagcaagaga ggaaatttca cgttcgggac
    15001 tggcgctgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
    15061 atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc ctcgcgcagc
    15121 agacagaacg gcgcaatcag cgggcgcagc aaattgccga aagacttgaa aaagcgcaaa
    15181 tacctggggc gctggaaggc gcacaacgac tggcgcaggg cggcgcagtg acgcgcggtc
    15241 attttgcgcg tttccttgtg gagtgcggca aagccagttc aatggcagat gtctttaaaa
    15301 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagaacaag
    15361 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
    15421 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac cacggcgacg
    15481 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg cacccagctg gccacacttg
    15541 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
    15601 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
    15661 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
    15721 taacccgcag caacgtctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
    15781 tgtctatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgccat
    15841 ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
    15901 cgatctttct gaactctcga cctattcatt cgtggataac gttgcgtttc gtttgatgaa
    15961 aaacaacacg ccgggcaact ataccttcat cctgaagggg acgaaggaag tgccacgccg
    16021 tctgttgcag gagaaacgca aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca
    16081 ggcgttgctt gaagcactgg gcgaaccgat gctttccact tcgctaatgc tgccaggcag
    16141 cgaatttacc gaatccgatc ccgaagaaat taaagaccgt ctggaaaaac aggtagattt
    16201 gattattcac ggcggctatc tcgggcagaa accaacaacg gttattgatc ttaccgatga
    16261 tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aagtgcgtaa
    16321 ctctgtatac tacgcggcct taaaatggcg ctatacggcg ccattgattc tcctgttcga
    16381 cgcctgtgaa ggcgacacca aaaggaagct ctatgagcga aaaattacag aaagtgctgg
    16441 cccgcgctgg ccacggctcc cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga
    16501 gtgttgatgg caaaattgct aaactcggcg atcgcgttga agttacccct gggctgaaaa
    16561 ttcgtatcga tggtcatctg atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc
    16621 tggcctatta caaaccggaa ggtgagttgt gtacccgtaa cgatccggaa gggcgtccga
    16681 cggtgtttga ccgtctgcca aagctgcgtg gcgcacgctg gattgccgta ggtcgtctgg
    16741 acgttaacac ctgtggtttg ctgctgttca ccaccgatgg tgaactggca aaccgtttaa
    16801 tgcatccaag ccgtgaagtt gaacgtgaat acgccgtgcg tgtatttggt caggttgacg
    16861 acgcgaaact gcgtgatttg agtcgtggcg tgcaactgga agatggtccg gcagctttta
    16921 aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg
    16981 aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc
    17041 tgatccgtgt tcgttacggt gatatcccac tgccaaaagg tctgccacgc ggtggctgga
    17101 cggagctgga cctcgcccag actaactatc tgcgcgaact ggtggagctt ccgccagaaa
    17161 ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat gaaggctaat cagattcgtc
    17221 gtgcggtgaa acgtcacagt caggtgagcg gcggtcgtcg ttctggcggg cgtaataacg
    17281 gttaatcaaa gtatgccgga cgtcatatcc ggcattttta cagattaata atcaatccct
    17341 atttgcgcct taacaccggc atcaaacgca tgtctgatgg ggcgcagctc acttaccgta
    17401 tccgccagtt caagaatatc ccgatgacag cctcgacccg tgataatcac cgtctgttga
    17461 tgtggacgtt catttaacgc ctgcaccact tcttccagtg gcaaatagtc atacgccacc
    17521 atatacgtca gttcatcaag caaaaccata tccagtgagg aatcagcaag catccgcttt
    17581 gcatgttgcc agacttcgcg gcaggcggcg gtatcagact cgcggttttg tgtatcccag
    17641 gtaaagcccg ttgccatcac ctgaaactca acgccatgtg gctccagcag attgcgttcg
    17701 ccattaggcc aggtgccttt aataaactgc acgacgccca ctttttttcc gtgaccaact
    17761 gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg
    17821 atgataatac cgcgttcatc ctgggcctgg gccacacgag catctacttt ttctttcact
    17881 cgctgctgac gctgttggta gcgttcatca ctcattggga aattcctggt ttacggcccg
    17941 gttgggcgtc aaaggtcatg ccggttttac ggcggctgtc atcgcccatc agccagaggt
    18001 agagcggcat gatatcagcg ggtgttttaa gtttctgtgg atcttcggtc gggaaggcgc
    18061 ttgcgcgcat tgcggtgcgc gtaccgcctg ggttaatgca gttgacgcgc aggcgctgct
    18121 gatattcatc ggccagtacc tgcatcatcc cttcggtggc aaatttcgac gctgcatatg
    18181 caccccagtt ggctcgtccc tgacgtccaa cgcttgatga agtaaagacc agtgaaccgg
    18241 cgtccgattt gagtaataaa ggaagcagtg cctgggtgag cataaaggtg gcattaacgt
    18301 tgacctgcat gacgtcctgc cagacctgcg gattttgttc gctcattggg caaacatcgc
    18361 cgagcaatcc ggcattatgc aaaacaccat ccagacgcgg ataattaacg acaatgcgct
    18421 gtgccagttg ttggcaattt tcggacgtgc aggtcagcaa atcgagaata aaccactgtg
    18481 gctgacgccc agtttcttcg tttatgtggc tggctacctg acgtaatttt tcttcattac
    18541 ggcccaacag aatcactgtc gcaccatagc gtgcatacgt catcgcggct tcacgaccaa
    18601 tcccatcgct ggctcccgtc accaggataa tgcgatcatt gagtaaatct tgttttggct
    18661 ggtaatgcat ggctactcct caacgacgtt gtctgtcgcg ctgccagtgt actttatgac
    18721 tttggggctt tatgcctgaa acagaagggc atttcaatca gcctggggcg ataacaccgc
    18781 aaaatggata ctttgtcata ctttcgctgc aataacatct ctgcgagacg gcttaacatg
    18841 cctgttgtaa actgtgagcc aaagcgttgt ttaaccaagg tggggactcg tggaattgtt
    18901 gtctgaatat ggtttgtttt tggctaaaat cgttaccgtt gtgctagcga ttgcggcgat
    18961 tgccgccatt attgtcaatg ttgctcaacg taataaacgc cagcgtggcg agttacgggt
    19021 caacaatctc agcgaacagt ataaggagac gaaagaagaa ctggccgcgg cgctgatgga
    19081 ctcacatcag caaaaacagt ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa
    19141 agcagcaaaa gcgaaagcca aactgggcga ggtggcaact gacagtaaac ctcgcgtctg
    19201 ggtgctggat tttaaaggca gcatggacgc ccatgaagtg aactcgctac gtgaagagat
    19261 aacggcggta ctcgcagcat tcaaaccgca ggatcaggtt gtgctccgtc tggaaagccc
    19321 tggtggcatg gtgcatggtt acgggttggc ggcttcgcag ctgcagcgtc tgcgtgataa
    19381 aaacattcct ttaactgtta cggtagacaa agtcgctgcc agcggcggtt acatgatggc
    19441 ctgtgtggcg gacaaaattg tttccgcacc gtttgctatt gtgggttcca ttggggtggt
    19501 ggcgcaaatg cccaacttta accgcttcct gaaaagcaaa gatattgata tcgaactgca
    19561 caccgccggg cagtataagc gcacgctgac cttgctgggt gaaaataccg aagaagggcg
    19621 ggagaaattc cgcgaagagc tgaacgaaac gcatcagtta tttaaagatt ttgtgaagcg
    19681 tatgcgtccg tctctggata ttgaacaggt ggcaacgggt gaacactggt acggacaaca
    19741 ggcggtagag aaaggcctgg ttgatgaaat caacaccagt gatgaagtta ttcttagcct
    19801 gatggaaggc cgtgaagtgg ttaatgtacg ctatatgcag cgtaaacgac tcattgaccg
    19861 attcaccggc agcgcggcag agagcgccga tcgattgttg ctacgctggt ggcagcgggg
    19921 tcaaaagcca ttgatgtaaa agacaaacgc gaggctaaga cctcgcgttt tgctttaatc
    19981 aaccagatga tatttttctg aaagcacatg ggccaggtgt ttgaacatat taaacaccgc
    20041 ggtgcttttg gctgttggca atccttgttc atctaaaaag tagtcgccgg taaataccag
    20101 cacgccatta cgctgcgtga cgccggtggc ttcaatccct gcgagcgtat cctcatgctc
    20161 acgaatgatt ttgttagcct ctttcaacag cgtttcgcgg tcgatgggtt gtgtctcttt
    20221 gttcattatt tactccttaa acaaggacat tagtctacgc caggcatggc ttgcagacaa
    20281 atataccacg ctggtggcaa gagcgcctta ctggcaactt tggattttgc atgctaataa
    20341 agttgcgtat cggattttat caggtacagt gtgacgcttt cgtcaatctg gcaatagatt
    20401 tgcttgacat tcgaccaaaa ttccgtcgtg ctatagcgcc tgtaggccaa gacctgttaa
    20461 ctcagtcacc tgaattttcg tgaacagagt cacgacaagg gggtgatatc cgcagagagc
    20521 gagtccatat cggtaactcg ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg
    20581 aagaatcaaa ttaggtaagg tgaatatggg taaagctctt gtcatcgttg agtccccggc
    20641 aaaagccaaa acgatcaaca agtatctggg tagtgactac gtggtgaaat ccagcgtcgg
    20701 tcacatccgc gatttgccga ccagtggctc agctgccaaa aagagtgccg actctacctc
    20761 caccaagacg gctaaaaagc ctaaaaagga tgaacgtggc gctctcgtca accgtatggg
    20821 ggttgacccg tggcacaatt gggaggcgca ctatgaagtg ttgcctggta aagagaaggt
    20881 cgtctctgaa ctgaaacaac tggctgaaaa agccgaccac atctatctcg caaccgacct
    20941 tgaccgcgaa ggggaagcca ttgcatggca cctgcgggaa gtgattggtg gtgatgatgc
    21001 gcgctatagc cgagtggtgt ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa
    21061 caaaccgggt gagctgaata ttgatcgtgt taatgcccag caggcgcgtc gctttatgga
    21121 ccgcgtggtg gggtatatgg tttcgccgct gctgtggaaa aagatcgctc gtggtctgtc
    21181 tgccggtcgt gtgcagtcgg tggcggtccg cctggtggta gagcgtgagc gtgaaattaa
    21241 agcgttcgta ccggaagagt tctgggaagt cgatgccagc acgactacgc catctggtga
    21301 agcgttggcg ttgcaggtga ctcatcagaa tgacaaaccg ttccgtccgg tcaacaaaga
    21361 acaaactcag gctgcggtaa gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga
    21421 agacaaaccg acaaccagta aacctggcgc tccttttatt acctctacgc tgcaacaagc
    21481 tgccagcacc cgtcttggtt ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta
    21541 tgaagcaggc tatatcactt acatgcgtac cgactccact aacctgagtc aggacgcggt
    21601 aaatatggtt cgcggttata tcagcgataa ttttggtaag aaatatctgc cggaaagtcc
    21661 gaatcagtac gccagcaaag aaaactcaca ggaagcgcac gaagcgattc gtccttctga
    21721 cgtcaatgtg atggcggaat cgctgaagga tatggaagca gatgcgcaga aactgtacca
    21781 gctaatctgg cgtcagttcg ttgcctgcca gatgacccca gcgaaatatg actccacgac
    21841 gctgaccgtt ggtgcgggcg atttccgcct gaaagcacgc ggtcgtattt tgcgctttga
    21901 tggctggaca aaagtgatgc ctgcactgcg taaaggcgat gaagatcgta tcttaccggc
    21961 agtcgataaa ggcgatgctc tgacgctcgt tgaactaaca ccagcccagc actttaccaa
    22021 gccgccagcc cgtttcagtg aagcatcgct ggttaaagaa ctggaaaaac gtggtatcgg
    22081 tcgtccgtct acctatgcgt cgatcatttc gaccattcag gatcgtggct atgtgcgagt
    22141 agaaaatcgt cgtttctatg cggaaaaaat gggcgaaatc gtcaccgatc gccttgaaga
    22201 aaatttccgc gagttaatga actacgactt taccgcgcag atggaaaaca gcctcgacca
    22261 ggtggcaaat cacgaagcag agtggaaagc tgtactggat cacttcttct cggatttcac
    22321 ccagcagtta gataaagctg aaaaagatcc ggaagagggt ggtatgcgcc cgaaccagat
    22381 ggttctgacc agcattgact gcccgacttg tggtcgcaaa atggggattc gcacagcgag
    22441 caccggggta ttccttggct gttctggcta tgcgctgccg ccgaaagagc gttgcaaaac
    22501 caccattaac ctggtgccgg aaaacgaagt gctgaacgtg ctggaaggcg aagatgctga
    22561 aaccaacgcg ctgcgcgcaa aacgtcgttg cccgaaatgc ggcacggcga tggacagcta
    22621 tctcattgat ccgaaacgta agttgcatgt ctgtggtaat aacccaacct gcgacggtta
    22681 cgagatcgaa gagggcgaat tccgcattaa aggttatgac ggcccgatcg ttgagtgtga
    22741 aaaatgtggc tctgaaatgc acctgaaaat ggggcgtttc ggtaaataca tggcctgcac
    22801 caacgaagag tgtaaaaaca cacgtaagat tttacgtaac ggcgaagtgg caccaccgaa
    22861 agaagatccg gtgccattac ctgagctgcc gtgcgaaaaa tcagatgctt atttcgtgct
    22921 gcgtgacggt gctgccggtg tgttcctggc tgccaacact ttcccgaaat cgcgtgaaac
    22981 gcgtgcgcca ctggtggaag agctttatcg cttccgcgac cgtctgccgg aaaaactgcg
    23041 ttatctggcc gatgcgccac agcaggatcc ggaaggtaat aagaccatgg ttcgctttag
    23101 ccgtaaaacc aaacagcaat atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc
    23161 agcattttat gttgatggca aatgggttga aggaaaaaaa taacctttaa ttctatcagg
    23221 tttttataaa caaagggtcg cgaaagcggc ccttttttat tgcatattat tttttcttca
    23281 cacctataca ctaaggctat aaatgatata gtggttatag ttagcacctt ttttattatt
    23341 aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg gcgtctaagt
    23401 ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt caatcataac
    23461 ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat cagtaaacaa
    23521 gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg caagcacctg
    23581 acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt cctgtcgaaa
    23641 gtcgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg ctcgctgtat
    23701 atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa aggctttatt
    23761 gagcgttatc cccgcgtttc tttgcatatg caccagggcc cgccgacaca aattgctgat
    23821 gccgtctcta aaggcaatgc cgatttcgct atcgccacag aagcgctgca tctgtatgaa
    23881 gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac tccggatcac
    23941 ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc gttggtgaca
    24001 tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg cgcagggtta
    24061 acgccgcgta tcgttttcac ggcaacggat gctgacgtca ttaaaactta cgtccggtta
    24121 gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga tcccgacctt
    24181 gtgcgcgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg ttttcgccgt
    24241 agtacttttt tgcgcagtta tatgtatgat ttcattcagc gttttgcacc gcatttaacg
    24301 cgtgatgtcg ttgatgcggc tgtcgcattg cgctccaatg aagaaattga ggtcatgttt
    24361 aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc ttttatgtag
    24421 cgaaggtgcc ggaatatatt ctcttttgtt acttatttct ataccgtgca caatattttt
    24481 atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc cgcctcgcaa
    24541 attccagttt tcaaatatct atttcaggtc aaaagattga attattcact ggagacgatt
    24601 cgtaaattcg ctggattttt gactaaagtt tctttaggat ttgtctcatc gtatgattaa
    24661 ttacaccaat cattcaacgt cttgctgata agtgatggcg attatatgag gttagcaatg
    24721 ccttctggaa atcaggaacc ccgccgtgat cctgaattaa aacgtaaagc ctggctgacg
    24781 gtttttcttg gttctgcact tttctgggtg gttgtcgcac tgctgatttg gaaagtgtgg
    24841 ggataactat ggtcggtcag gagcaactgg agtcgtcacc attatgccag catagtgaca
    24901 atgaaccaga aacgaaacgg gaatgttccg tcgttattcc agacgactgg caactaacat
    24961 cgcagcagca agcctttata gaactgtttg ctgaagatga tcagccgaaa caataattat
    25021 catcattctt attacccatt tttaatgaat taaagggctt ttaatacacc gcagcaataa
    25081 cagcttgagt tatctcaaca caaaataata accgttaagg gtgtagccta tgatcaacac
    25141 aaatatgaaa tattggtcct ggatgggcgc gttttctctg tcgatgctct tctgggccga
    25201 actcctctgg atcattactc actgatcctt gaccccgctg cggcggggtt gtcatttgct
    25261 ttgccacaag gtttctcctc ttttatcaat ttgggttgtt atcaaatcgt tacgcgatgt
    25321 ttgtgttatc tttaatattt accctgaaga gaatcagggc ttcgcaaccc tgtcattaag
    25381 gaggagctat gtcgtcaacc ctacgagaag ccagtaagga cacgttgcag gccaaagata
    25441 aaacttacca ctactacagc ctgccgcttg ctgctaaatc actgggcgat atcacccgtc
    25501 tacccaagtc actcaaagtt ttgctcgaaa acctgctgcg ctggcaggat ggtaactcag
    25561 ttaccgaaga ggatatccac gcactggcgg gatggctgaa aaatgcccat gctgaccgtg
    25621 aaattgccta ccgcccggca agggtgctga tgcaggactt taccggcgta cctgccgttg
    25681 ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg tggcgatact gcaaaggtta
    25741 acccgctctc accggtcgac ctggtcattg accactcggt gaccgtcgat cgttttggtg
    25801 atgatgaggc atttgaagaa aacgtacgcc tggaaatgga gcgcaaccac gaacgttatg
    25861 tgttcctgaa atggggaaag caagcgttca gtcggtttag cgtcgtgccg ccaggcacag
    25921 gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc agtgtggagc gaattgcagg
    25981 acggtgaatg gattgcttat ccggatacac tcgttggtac tgactcgcac accactatga
    26041 tcaacggcct tggcgtgctg gggtggggcg tgggtgggat cgaagcagaa gccgccatgt
    26101 taggccagcc ggtttccatg cttatcccgg acgtagtggg ctttaaactt accggaaaat
    26161 tacgtgaagg tattaccgcc acagacctgg ttctcactgt tacccaaatg ctgcgcaaac
    26221 atggcgtggt ggggaaattc gtcgaatttt atggtgatgg tctggattca ctaccgttgg
    26281 cggatcgcgc caccattgcc aatatgtcgc cagaatatgg tgccacctgt ggcttcttcc
    26341 caatcgatgc agtaaccctc gattacatgc gtttaagcgg gcgcagcgaa gatcaggtcg
    26401 agttggtcga aaaatatgcc aaagcgcagg gcatgtggcg taacccgggc gatgaaccga
    26461 tttttaccag tatgttagaa ctggatatga atgacgttga agcgagcctg gcagggccaa
    26521 aacgcccaca ggatcgcgtt gcactgcccg atgtaccaaa agcatttgcc gccagtaacg
    26581 aactggaagt gaatgccacg cataaagatc gcctgccagt cgattatgtt atgaacggac
    26641 atcagtatca gttacctgat ggcgctgtgg tcattgctgc gataacctcg tgcaccaaca
    26701 cctctaaccc aagtgtgctg atggccgcag gcttgctggc gaaaaaagcc gtaactcttg
    26761 gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc gggttcgaaa gtcgtttctg
    26821 attatctggc aaaagcgaaa ctgacaccgt atctcgacga actggggttt aaccttgtgg
    26881 gatacggttg taccacctgt attggtaact ctgggccgct gcccgatcct atcgaaacgg
    26941 caatcaaaaa aggcgattta accgtcggtg cggtgctgtc cggcaaccgt aactttgaag
    27001 gccgtatcca tccgctggtt aaaactaact ggctggcctc gccgccgctg gtggttgcct
    27061 atgcgctggc gggaaatatg aatatcaacc tggcttctga gcctatcggc catgatcgca
    27121 aaggcgatcc ggtttatctg aaagatatct ggccatcggc acaagaaatt gcccgtgcgg
    27181 tagaacaagt ctccacagaa atgttccgca aagagtacgc agaagttttt gaaggcacag
    27241 cagagtggaa ggaaattaac gtcacacgat ccgataccta cggttggcag gaggactcaa
    27301 cctatattcg cttatcgcct ttctttgatg aaatgcaggc aacaccagca ccagtggaag
    27361 atattcacgg tgcgcggatc ctcgcaatgc tgggggattc agtcaccact gaccatatct
    27421 ctccggcggg cagtattaag cccgacagcc cagcgggtcg atatctacaa ggtcggggtg
    27481 ttgagcgaaa agactttaac tcctacggtt cgcggcgtgg taaccatgaa gtgatgatgc
    27541 gcggcacctt cgccaatatt cgcatccgta atgaaatggt gcctggcgtt gaagggggga
    27601 tgacgcggca tttacctgac agcgacgtag tctctattta tgatgctgcg atgcgctata
    27661 agcaggagca aacgccgctg gcggtgattg ccgggaaaga gtatggatca ggctcaagcc
    27721 gtgactgggc ggcaaaagga cctcgtctgc ttggaattcg tgtggtgatt gccgaatcgt
    27781 ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgctg gaatttccgc
    27841 aaggcgtgac gcgtaaaacg ttagggctaa ccggggaaga gaagattgat attggcgatc
    27901 tgcaaaatct acaacctggc gcgacggttc cggtgacgct tacgcgcgcg gatggtagcc
    27961 aggaagtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgacc tactaccaga
    28021 acgacggcat tttgcattat gtcatccgta atatgttgaa gtaacaagta tttgcttgcc
    28081 ggttattttg cttccggcaa gcaaatgaat tacacaatgc aagagggtta tttgttcagc
    28141 aaatggccca ttttctcggc tttggtatcg agataatgtt cgttattggg gttacgacct
    28201 acaatcaatg gtacgcgttc aacaatatta atccctgctt cggtcagaat ttcgactttt
    28261 ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa catatcagcg
    28321 caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc ctctacggta
    28381 tcgtaacctt gatcctgcag tgcgtaagcg cggattttat tcagcagacc aatgttacga
    28441 ccttcctgac ggtgatacag caaaatacca cggccttcct cggcaatttg cgtcaatgcc
    28501 gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc ggtcagacat
    28561 tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcggcata gactagcgcg
    28621 acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc gccccatggg
    28681 gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag ataatgctgg
    28741 ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg acgcgtcgat
    28801 cgttcacgga atggcacaaa tctgtcaatt tttcttggaa ctggcatttt cagttatgat
    28861 tgtgggactt atcaaaaagg agaggccatg cgttcgattg cccgacgtac cgcagtggga
    28921 gctgcactat tgtttgtcat gccagtagcc gtatggattt ctggctggcg ttggcaacct
    28981 ggagaacaaa gttggcgact aaaagcggct ttttgggtta ctgaaactgt cacccagccc
    29041 tggggcgtca ttacacattt gattttgttc ggctggtttc tctggtgtct gcgttttcgc
    29101 attaaggctg cctttgtatt attttccatt ctggcggcgg caatccttgt tggacaaggc
    29161 gttaaatcct ggatcaaaga caaagtacag gaaccacgac cttttgttat ctggttggaa
    29221 aaaacacatc atattccggt tgatgagttc tacactttaa agcgagcaga acgcggaaat
    29281 ctagtaaaag aacagttggc tgaagagaaa aatattccac aatatttgcg ttcacactgg
    29341 cagaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc cagttgggca
    29401 ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt taaccattgc tatcttgttg
    29461 gtctgggcaa cgggagtcat gggaagccgc ctgctactcg ggatgcattg gccacgcgat
    29521 ctggtggtag ctacgttgat ttcgtggacg ctggtggcgg tggcaacgtg gcttgcgcaa
    29581 cgaatttgtg ggccattaac accacctgcg gaagaaaatc gcgaaattgc acaacgagaa
    29641 caagaaagtt aacgctggtt gattttccga atttaggcct taaatcatca acaatgcgcg
    29701 tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg cggtaagttg
    29761 accataattt attcgctcta accacataac gggaagtaat gtgaaatatt tactcatttt
    29821 cttactggtg ttagcgatct tcgtgatttc ggtcacgttg ggtgcgcaga acgatcaaca
    29881 ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat tgctggcggt
    29941 attgtttgct gcggggtttg ctatcggttg gttgatttgt ggcctgttct ggctgcgagt
    30001 tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga ctggaaaacc agctttcacc
    30061 cgcgactgac gtggctgtag tgccgcactc gtcagcggcg aaggaataac tttctatgct
    30121 ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata tgggccgcag
    30181 aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt acgtagcggg
    30241 ggttaacttc ctgcttagta atcaacagga taaagcggta gacctgtttc tcgatatgct
    30301 taaagaggat acaggcaccg ttgaagccca ccttacgctc ggaaacctgt tccgttcgcg
    30361 tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg cctcgctgac
    30421 ctatgaacag cgtctgttgg cgattcaaca actggggcgt gattacatgg ccgccgggtt
    30481 atacgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact tccgcattgg
    30541 cgcgctgcaa cagttgctac aaatctacca ggctaccagt gagtggcaga aagcaattga
    30601 tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa ttgcccattt
    30661 ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg ccatgacctt
    30721 gctaaaaaaa ggggcggcgg cagataaaaa cagcgcccgc gtatccatca tgatgggacg
    30781 cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa agtttgcaac gcgtcatatc
    30841 ccaggacaga gaactggtca gcgaaacgct ggaaatgttg caaacctgct atcagcagtt
    30901 gggtaaaact gccgaatggg cagaattcct gcaacgcgcg gtggaagaga acaccggtgc
    30961 cgatgctgaa ttgatgcttg ctgatatcat cgaagcgcgc gacggtagtg aggctgcaca
    31021 ggtctatatt acacgccagc ttcagcgtca tccgaccatg cgtgtattcc ataagctaat
    31081 ggattaccac ttaaatgaag cggaagaagg gcgcgccaaa gagagtctga tggtgctgcg
    31141 tgacatggtt ggcgagaagg tgcgtagtaa gcctcgttat agctgccaga aatgtggttt
    31201 taccgcatac accctctact ggcattgtcc gtcttgtcgt gcctggtcaa ctattaaacc
    31261 gattcgcggt cttgatggcc tgtaattttt aaaaaaatcc gactttagtt acaacatact
    31321 agttattgat gtcccattgt gctccggcaa cgacggagca gaaaaagcct gctaggggaa
    31381 caatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt acctgtttcg
    31441 cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttagctg cttcatcttc
    31501 ttcccgcgct gttacgaatt ctcctgtggt tgttgccctt gattatcata attgtgatga
    31561 cgcgctgtcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg tcggtaaaga
    31621 gatgtttaca ttgtttgggc cacagtttgt gcgcgaactt caacagcgtg gttttgatat
    31681 ctttcttgac ctgaaattcc acgatatccc caacactgca gcgcacgctg tcgctgctgc
    31741 agctgactta ggcgtgtgga tggtgaatgc tcatgcctcc ggtggggcgc gtatgatgac
    31801 cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga ttgctgtgac
    31861 agtgttgacc agcatggaag ccagcgacct ggccgatctt ggcgtgacac tgtcacctgc
    31921 agattatgca gaacgtctgg cagcactgac gcaaaaatgt ggccttgatg gtgtggtgtg
    31981 ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca aactggttac
    32041 accgggcatc cgtccgcagg ggagtgaagc tggtgaccag cgccgcatta tgacgccaga
    32101 acaggcgttg gcggctggtg ttgattatat ggtgattggt cgcccggtaa cgcaatcggt
    32161 agatccagcg cagacactga aagcgatcaa cgcctcttta cagcggagtg catgatgagt
    32221 gattccaaca gccgtctggt ctactcaacg gaaaccgggc gtattgatga gcccaaagcg
    32281 gccccagtac gccctaaagg cgatggagcg gtgcgtattc agcgtcagac cagtggacgt
    32341 aaaggtaagg gcgtttgcct gattaccggt atcgatctcg atgatgccga actgacaaaa
    32401 cttgcagcgg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga tggagttatt
    32461 gaaatccagg gcgataagcg tgatttatta aaatcactac ttgaagcgaa agggatgaag
    32521 gtaaaactcg caggcggtta acataaaaag ccacggatat atccgtggct ttcgaatatt
    32581 ttactgtgtg tattatt
//
LOCUS       DAJTYI010000031        32582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYI010000031 DAJTYI010000000
VERSION     DAJTYI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            17..4549
                     /locus_tag="OO564_001443"
     CDS             17..4549
                     /locus_tag="OO564_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6652617.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4917..6572
                     /gene="lldP"
                     /locus_tag="OO564_001444"
     CDS             4917..6572
                     /gene="lldP"
                     /locus_tag="OO564_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6652618.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6572..7348
                     /gene="lldR"
                     /locus_tag="OO564_001445"
     CDS             6572..7348
                     /gene="lldR"
                     /locus_tag="OO564_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6652619.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7345..8535
                     /gene="lldD"
                     /locus_tag="OO564_001446"
     CDS             7345..8535
                     /gene="lldD"
                     /locus_tag="OO564_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6652620.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8731..9204
                     /gene="trmL"
                     /locus_tag="OO564_001447"
     CDS             8731..9204
                     /gene="trmL"
                     /locus_tag="OO564_001447"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6652621.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKHL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO564_001448"
     CDS             complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO564_001448"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6652622.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO564_001449"
     CDS             complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO564_001449"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6652623.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO564_001450"
     CDS             complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO564_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6652624.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO564_001451"
     CDS             complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO564_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6652625.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12100..12531)
                     /locus_tag="OO564_001452"
     CDS             complement(12100..12531)
                     /locus_tag="OO564_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6652626.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12776..14320
                     /gene="gpmM"
                     /locus_tag="OO564_001453"
                     /gene_synonym="pgmI"
     CDS             12776..14320
                     /gene="gpmM"
                     /locus_tag="OO564_001453"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6652627.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14330..15613
                     /gene="envC"
                     /locus_tag="OO564_001454"
     CDS             14330..15613
                     /gene="envC"
                     /locus_tag="OO564_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6652628.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15617..16576
                     /locus_tag="OO564_001455"
     CDS             15617..16576
                     /locus_tag="OO564_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6652629.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO564_001456"
     CDS             complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO564_001456"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6652630.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO564_001457"
     CDS             complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO564_001457"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6652631.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO564_001458"
     CDS             complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO564_001458"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6652632.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO564_001459"
     CDS             complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO564_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6652633.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21503..22435
                     /gene="rfaD"
                     /locus_tag="OO564_001460"
     CDS             21503..22435
                     /gene="rfaD"
                     /locus_tag="OO564_001460"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6652634.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22445..23491
                     /gene="rfaF"
                     /locus_tag="OO564_001461"
     CDS             22445..23491
                     /gene="rfaF"
                     /locus_tag="OO564_001461"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6652635.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23495..24487
                     /gene="rfaC"
                     /locus_tag="OO564_001462"
     CDS             23495..24487
                     /gene="rfaC"
                     /locus_tag="OO564_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6652636.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24484..25692
                     /gene="rfaL"
                     /locus_tag="OO564_001463"
     CDS             24484..25692
                     /gene="rfaL"
                     /locus_tag="OO564_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6652637.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25728..26870)
                     /locus_tag="OO564_001464"
     CDS             complement(25728..26870)
                     /locus_tag="OO564_001464"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6652638.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26879..27892)
                     /locus_tag="OO564_001465"
     CDS             complement(26879..27892)
                     /locus_tag="OO564_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6652639.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO564_001466"
     CDS             complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO564_001466"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6652640.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO564_001467"
                     /gene_synonym="rfaI"
     CDS             complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO564_001467"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6652641.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO564_001468"
     CDS             complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO564_001468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6652642.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30490..31614)
                     /locus_tag="OO564_001469"
     CDS             complement(30490..31614)
                     /locus_tag="OO564_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6652643.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31611..>32582)
                     /gene="rfaQ"
                     /locus_tag="OO564_001470"
     CDS             complement(31611..>32582)
                     /gene="rfaQ"
                     /locus_tag="OO564_001470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6652644.1"
                     /translation="KKNYPDAKIDVLLYQDTIPILSENPEINALYGIKNKKAKASEKI
                     ANFFHLIKVLRANKYDLIVNLTDQWMVAILVRLLNARVKISQDYHHRQSAFWRKSFTH
                     LVPLQGGNVVESNLSVLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQ
                     PTARQIFKCWDNAKFSAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTAL
                     AGKVTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQ
                     FWAGDYREMPTRDQRDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL"
BASE COUNT         8585 a         7981 c         7715 g         8301 t
ORIGIN      
        1 atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg cgacagggaa
       61 ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct
      121 gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc
      181 cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg acgtctgggt
      241 tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt ccagtactgc
      301 tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt caaaaaccca
      361 tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag gcgatagaag
      421 tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc tggggcgtag
      481 cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc
      541 gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa
      601 taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa
      661 agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat
      721 aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc
      781 gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct
      841 tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc
      901 cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt
      961 taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct
     1021 ttatgccatt agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga
     1081 tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg
     1141 ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata
     1201 cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg
     1261 cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga
     1321 cgatgtcgaa gccaacacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     1381 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     1441 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     1501 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     1561 cgccaataac acttccaata ttgccactaa caccaccaac atctctaacc tgactgagac
     1621 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     1681 agctcatggc accgagacca ccagcaaaat aaccaacgtt aaagatggcg acctgacgac
     1741 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     1801 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     1861 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     1921 tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     1981 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     2041 cggcggcaat gcttctgtga atactgacgg cgtgtttacc ggtccaacct acaaaatcgg
     2101 tgaaacaaat tattataacg tcggcgatgc tctgctttgg gatgccaccg caggtaaatt
     2161 cagtgccaaa cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga
     2221 aatttcagac tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta
     2281 tgttgttgat gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc
     2341 gacgtacacc attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga
     2401 taccactcct gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag
     2461 cgccgctcac ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat
     2521 ctctgctgcc agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat
     2581 cgctgatgcg ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac
     2641 ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga
     2701 taacgccctg ctgtgggata agactgccaa tggcggtgct ggagcctaca atgccagcca
     2761 tgacggtaaa gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac
     2821 cgatgcagtg aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca
     2881 aattatcaat cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg
     2941 tatcaactac gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg
     3001 tgtcggcgct acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat
     3061 tggtcagaac agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc
     3121 cagccgtgtg attgccaaag gttctcgtga cactagcgta acggaaaatg gcttggtgat
     3181 tggttatgac accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa
     3241 ataccgccaa atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg
     3301 ccagttgcag aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa
     3361 ctcaacggca gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac
     3421 tgtcgttaat ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc
     3481 gatcaacggc attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat
     3541 gggtaacggt tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga
     3601 cgcaccgcag aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat
     3661 cacaaacgtc gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt
     3721 cactgatgag cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac
     3781 taatctggat actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg
     3841 tagcaccaag tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga
     3901 tagcgttgct attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac
     3961 cggttccgtt gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg
     4021 tatcaccaac gtagctgcca gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa
     4081 atcttctgaa gcaggcggag tgcgctacga caccaaagct gatggttcta tcgactatag
     4141 caatatcacc ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg
     4201 cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag caaagcgcgc aagaaacgaa
     4261 gcaatacacc gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa
     4321 gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc
     4381 gggtgccagc atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt
     4441 aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa
     4501 tagccagggt gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa
     4561 gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat
     4621 aaggcgctcc gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta
     4681 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
     4741 tcacaattcc ccaattaaaa ctcacatcaa tattgataat acataacatt tagttaacca
     4801 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
     4861 aaggagatga gcaacagact cattacacga tgtgcgtgga ctctaggaga cctgcaatga
     4921 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
     4981 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
     5041 ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
     5101 tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
     5161 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
     5221 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
     5281 gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta
     5341 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
     5401 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
     5461 aagtaacagg catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta
     5521 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
     5581 agacgtggcc tgcggtcgtg gttgcaggcg gctcgtttgc catcgctcag taccttagct
     5641 ctaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
     5701 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
     5761 cgtcacaggt tgatataacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
     5821 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
     5881 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
     5941 acctcaataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
     6001 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
     6061 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
     6121 tgaaagaact agctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
     6181 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
     6241 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
     6301 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
     6361 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
     6421 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
     6481 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
     6541 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
     6601 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
     6661 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
     6721 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
     6781 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
     6841 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
     6901 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
     6961 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
     7021 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
     7081 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
     7141 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
     7201 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
     7261 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa
     7321 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
     7381 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
     7441 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
     7501 aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
     7561 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
     7621 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
     7681 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
     7741 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
     7801 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
     7861 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
     7921 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
     7981 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
     8041 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
     8101 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
     8161 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
     8221 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
     8281 cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc
     8341 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa
     8401 aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
     8461 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
     8521 gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg
     8581 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
     8641 ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct
     8701 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
     8761 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctacat
     8821 atcatcgaac cgatgggatt tgcctgggac gacaagcacc tgcgccgcgc ggggctggac
     8881 tatcacgagt ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa
     8941 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc
     9001 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
     9061 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
     9121 atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
     9181 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
     9241 gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga
     9301 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
     9361 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
     9421 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
     9481 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
     9541 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
     9601 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
     9661 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
     9721 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
     9781 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
     9841 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
     9901 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
     9961 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    10021 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    10081 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    10141 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataat
    10201 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    10261 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    10321 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    10381 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    10441 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    10501 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    10561 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    10621 atgaaatccg ggttgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    10681 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    10741 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    10801 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    10861 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    10921 tggctgggta cgacgacgag aatattacga ctggctgcca gcgcagtggc gagatcgctt
    10981 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    11041 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    11101 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    11161 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    11221 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    11281 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    11341 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    11401 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc caaggaggcc gttacggtaa
    11461 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    11521 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    11581 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    11641 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    11701 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    11761 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    11821 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct ggaaactcac
    11881 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    11941 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    12001 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    12061 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    12121 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    12181 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    12241 aaccgatttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    12301 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    12361 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    12421 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    12481 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    12541 caactcccaa cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    12601 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    12661 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    12721 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    12781 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    12841 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    12901 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    12961 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    13021 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tggctggcgc
    13081 ggtacataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    13141 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    13201 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    13261 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    13321 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    13381 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    13441 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    13501 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    13561 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    13621 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    13681 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    13741 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    13801 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    13861 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    13921 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    13981 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    14041 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    14101 cgctgaccac ggtaacgctg aacagatgcg cgatccagca acgggtcagg cacacacggc
    14161 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc
    14221 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    14281 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    14341 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    14401 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    14461 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcacg cggtacgcca
    14521 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    14581 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    14641 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    14701 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    14761 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    14821 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcaccatgca
    14881 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    14941 ccaacaggcg aaactgactc aggttctgaa cgagcgtaaa aagacgctgg cggggctgga
    15001 atcttccatc cagcaaggtc agcagcagct aagtgaacta cgtgccaacg aatcccgtct
    15061 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    15121 tgaggcccag gcggttcgcg agcgccagaa agaagcgacg cgcaaaggca ccacctacaa
    15181 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    15241 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    15301 tgaattacgc tggaaaggga tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat
    15361 tgccgacggt cgagtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    15421 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    15481 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    15541 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    15601 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    15661 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    15721 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    15781 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt
    15841 gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctacg
    15901 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    15961 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    16021 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    16081 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    16141 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    16201 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    16261 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    16321 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    16381 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    16441 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    16501 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cacgaagcgg cgaagtttaa
    16561 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    16621 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    16681 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    16741 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    16801 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    16861 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    16921 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    16981 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    17041 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    17101 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    17161 acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    17221 aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc catggtcatc
    17281 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    17341 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    17401 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    17461 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    17521 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    17581 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    17641 tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt
    17701 cagggtagag agataatgag aacagctata taaaagatca gcgcaattac gaactcttta
    17761 ttacactgac aggattttac ataattaaca gattatcctc agataaaagg gctggaatac
    17821 cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc ccgaacgcat
    17881 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    17941 atcgaggcca gactgaatca gcgccgccat cttgtaccaa gtttcaaaca tctcacgacc
    18001 gtaaatgcct ttaatgaaca agcctttaaa gatcactttg gtccagtcga tagacatatc
    18061 agacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt
    18121 acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    18181 cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc
    18241 cattttgcgc gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc
    18301 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag
    18361 cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc
    18421 aaaaatggaa gccaggtcgt cggagatatt gtcggggatt ttgaaagcgt tgaacgccgg
    18481 aatcaccaga tattcggcga agcagcccgg acggttaaca ccgacaccaa tggtgttgcg
    18541 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    18601 gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc
    18661 accgacatat tcatggccca cgaccatcgg caccggaatg gttttttgcg accactcatc
    18721 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    18781 atcgttatgc ccgagttccg gtacagggac gtcggtcatc cagatgccct cttccgcttt
    18841 cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat
    18901 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    18961 ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtagat
    19021 gccctctttt tgcagctctc gggcaaattt ctgcgccact accgcatcac caagcatgac
    19081 cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    19141 gaactgacgc gcgttcgccc acagacggtc acgcaactcg ctacccgctt caaccatctc
    19201 cagcactttg atggaagcgg cgacaatcgc tggtgccagc gagttggaga acaggtacgg
    19261 acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat aaccaccaga
    19321 agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc ccatcacatc
    19381 gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc
    19441 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca gattggcaat
    19501 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    19561 acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg
    19621 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    19681 aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga
    19741 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    19801 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    19861 cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga gatagttgtt
    19921 ggcacaaaag ttaatgacgt ggcttccatc agccacggtg atatctgctt gctgcgcaga
    19981 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    20041 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    20101 tattacccaa acttataggt cgcgacgaga tgatgcgtta acacttctgc aaaattcagg
    20161 ataaataacg tgtatcttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt
    20221 tttggctgag cataatgatg agtgatttta ctgcattgat ataaaaagaa aaaatgatgc
    20281 caaagcatca tttttcctat ctcagttggc atcaaaacgc tacccactaa tggagggaaa
    20341 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    20401 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    20461 cggaacgttt gtaatagcac atgacaaaaa tgccctggtc atcgtccaca atgttattgt
    20521 ttagtgttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    20581 aaccaacaat tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaagcct
    20641 gttgttgaga tgtaacagta agcccttttt ttatagtgaa aagatgcatc ttattttcat
    20701 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    20761 aatctatcca cgcaacaaga ggcgttttca ctaaacccat gttgatagct ttatttacaa
    20821 aataggactt aaggttacat accaaaacat actctggtga ccagtactct gggtttttta
    20881 attgtcgagg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    20941 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    21001 tgcgaatcgc ttcaatcctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt
    21061 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    21121 cacggaaccc tttattagta gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
    21181 ttgtggtgga tgatttcatt actaataaga ccattaatat aaaaaaagaa ttatatcata
    21241 aatattaaca gaatgtatac tgtatattat ccattcaaat gattccgtta tttatcatac
    21301 atcggaatat tgatactaaa gcactattca catgcaaaac caacatccgc catgaaggac
    21361 tagctaaaac ccaaactagt ttgttgcaat tagcatcctt gcacctctat gtaaagggct
    21421 gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact
    21481 ccataattcg aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag
    21541 caacatcgtt aaggccctga atgataaagg catcaccgat attctggtgg tggacaacct
    21601 gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa
    21661 ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggtgatgtcg aagcgatttt
    21721 ccacgaaggc gcatgctctt ccaccaccga gtgggatggc aagtatatga tggataacaa
    21781 ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta
    21841 tgcctcttcc gcagccacct acggcggacg cacctccgac tttattgaat cccgcgagta
    21901 cgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgtca
    21961 aatcctgcca gaagcgaact cgcagattgt tggcttccgc tatttcaacg tttatggacc
    22021 gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa
    22081 taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta
    22141 tgtgggcgac gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggtatctt
    22201 caacctcggc accggtcgtg cagaatcctt ccaggcagta gcagatgcta cgctggctta
    22261 tcacaagaaa ggccagatcg aatacattcc gttcccggat aagctgaaag gccgctacca
    22321 ggcgttcact caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa
    22381 aaccgttgct gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct
    22441 ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag
    22501 tctctatcgc acgctccagg cgcgctatcc ccaggcgata atcgatgtga tggcaccggc
    22561 atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagctatcc ctatgcctct
    22621 cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa
    22681 gcgctacgac cgcgcctacg tcttaccaaa ctccttcaaa tctgcattag tgcctttctt
    22741 cgcgggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga
    22801 tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc
    22861 ctatgacaaa ggcattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca
    22921 gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga
    22981 acgtccgatg attggctttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca
    23041 ctaccactat gcggaactgg caaagcagct gattgatgaa ggttatcagg tggttctgtt
    23101 tggctctgcg aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca
    23161 gcaggcatgg tgtcggaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat
    23221 tgcagcctgt aaagccattg tcactaacga ttctggcctg atgcacgttg cggcggcgct
    23281 caatcgtccg ctggttgccc tgtatggtcc aagtagcccg gacttcacac cgccgctatc
    23341 ccataaagcg cgcgtgatcc gtctgattac cggctatcac aaagtgcgta aaggtgacgc
    23401 tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact
    23461 caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc
    23521 gtcgatgggc gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaatccc
    23581 agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc
    23641 tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc
    23701 ccccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcagaga actatgacgc
    23761 agttatcgac gctcaggggc tggtaaaaag cgcggcactg gtgacacgtc tggcgcatgg
    23821 cgtaaagcat ggattggact ggcaaaccgc tcgcgaacct ttagccagcc tgttttacaa
    23881 ttgtaagcat catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc
    23941 caaaagtttg ggctatagca aaccgcaaac ccagggcgat tatgctatcg cacagcattt
    24001 tctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg
    24061 tgatgataaa cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc
    24121 aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact
    24181 ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg
    24241 cgtgctggcc ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc
    24301 ggcactggat agacccaata tcacggttta tggaccaacc gatccgggat taattggtgg
    24361 gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc
    24421 tcaaaccgtt tttcaaaatt taaatcttga aataataacc aataagttga catcggagat
    24481 aagatgacct caacattatt tttctctctc gagaaaaaaa actggatagc gtactggaac
    24541 agagctctcg tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa
    24601 catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa
    24661 cactatctct cactatttaa aacatttctt tttggtagtg ttgccatatt aactattgct
    24721 gcattgctgt cacttcttca atcccctgat gcaggtgcta gcatgaagga aattttcaaa
    24781 gctattattg agaatacttt actatgcaca atagcaatac cggtcatatt gagagacgag
    24841 aaaagagaag atgtcgaaaa aatcgttttc ttctcattta ttagtgcgtt gggcttacgc
    24901 tgtttttctg aattgattac ctattataag gactatcaac aagggataat gccattcgca
    24961 gattatagac accgtagcat ttctgactcg atggtctttt tattccctgc attgttaaat
    25021 ctctggctta tcaaatcagc aaaataccgc atttcttttg tggttctaag cgttattttt
    25081 atttttctga tattaggaac tttatccaga ggggcctggc tttccgtgtt agtcattgga
    25141 ttaatatgga ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc
    25201 atcattgcat tgtcggttat tttcacacat aaggagatga ccgcaaagct aacgtataaa
    25261 cttcaacaaa ctaatagttc ttatcgctat gcaaatggta ctcaaggcag cgcactcgat
    25321 ctaatattag aaaatcctgt tattggttat ggttacggta acgttgcata taaagatgtc
    25381 tataataaac gtgtcattga ttatccagaa tggaccttta gacaatcaat agggccacat
    25441 aattttgcgc tattcatctg gtttggcact ggtttattag ggctggtaag tcttatgatg
    25501 ctatactgtg caatattgaa agagtgtata aaaaatggcg tcaagaataa atatcgctca
    25561 ccatataatg catattatat aatcttacta tcttttatag gttattttgt tatccgtgga
    25621 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
    25681 aaaaacaagt aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
    25741 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg atcaaacaca
    25801 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
    25861 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
    25921 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
    25981 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
    26041 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
    26101 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
    26161 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
    26221 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
    26281 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
    26341 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
    26401 taagattggt aatacttttt caagtataaa ctagggacta tcattttaac attttgctca
    26461 agcaattttg gttcaaatgc attatgcata tgtataacca ctcttgcctg aggagctcgc
    26521 ttacgaattt gagtgtatag cttcatgcta ttatgaacta caataacact ttctttcgtt
    26581 ataggaaaat catgtgcaat attcaatatt ctctgtgaat atggaagagg atcaagtctt
    26641 gtccattttt gaaataaccg cttatatatg cgactgaatc caaccctatg aatactacaa
    26701 ttatcattaa caaaggtgta atttgagtat cctggatttt tgatacaaac aatacgattt
    26761 ggaaaattgg tacgctgagc aacctgatac atccacgttt caacagcagc cgctccgcga
    26821 ggaggaattg aaaaaatagg agtaaccgta aatattattt tatcaaccat ctcgtatatt
    26881 aacctttcat aacattatat ttaattgctg taaaaacacc atcgagaaat tttttctggt
    26941 aaagcaagtg tttatatttt tctttgtgct catgtttttt aacagctttt ttataaggta
    27001 tatttctcca tggtgatata ttataaatat tacgaaaata atctgcagag gcgtagttcg
    27061 cccaatcatg ccatggctta gttataccag tataatgtat gaagacagtg tcatcattaa
    27121 taaaccgggt gtaatattca ctattttttt cttcaaattc tgactttata gtataaatac
    27181 aattatattt tctcggtaat attttagcat gatgtagaaa cattaagttt aaaatatctt
    27241 gatccgggta ctttaatttt tttataattg actcatctga taatagatca aagaattttt
    27301 ccgttagtct ttgttttaac cattcccgta aattaatata cattacacca gagttaaaat
    27361 agctaccgtt aaaatcctca ttacacaaac gctcagcgct tttactttgc atagcatcta
    27421 catcaattac cactgcccca tattcatctt taaactctaa tgctattaac tcgttcaatg
    27481 aacccttaca gacgatatca gcatcaagat ataataatga atccaatcgc gcagaaaaat
    27541 aatcaaatga aagcaaacga taataaatag atactggcca gatatttgat tgaggtagcg
    27601 ccttcaatgg ctcagaatca atatgatata atttaattac tgtttgatac ttcgttgcta
    27661 attgtgataa taattcaata tactctttgt ctaagtaatc agcgattatg tgaaaggtaa
    27721 atttgttttg tttattcttt tcaagaattg aagcaattga aatcgcagcc ccaacctggt
    27781 agttctcatc aataccccat gagacattaa acgtttcccg ttcatcaagt ttaacggggc
    27841 gcttgtccag ttcaattata tctttaaatt gagtaagatg tttaaaatcc aatttaatta
    27901 cccttcatca taatcattat ttgctcttca atcctagttt gacttttaca tccctgatta
    27961 cttttttaat tttctttttg aatataagat atgtatagcc aaagtctttc aattcatttt
    28021 ttatattata gtgccgttca agatcaatgc gatcttttgc ttttaaaaca gcggttgttt
    28081 ttttacctga aaggtcaatt agtcttaaac ctttttcgga tactataaaa tttcctttat
    28141 gaggatcgcc agacaccatt ccatgctgat gcaactcttt tatcgattct gataattgat
    28201 ccttcagatc ttcagaaatt tccaaatact catttaaccc gacaccttca atatactcaa
    28261 tgagcataat ataataatga gcgaaattta atgttttacg ttcagctagc aaaaaatagt
    28321 cattaattga ttggatgcct tcaccacgta cccgatcggt ttgataaatt aaattttcat
    28381 aataatcttt ttttatgcaa gattttaaga aacgttcggt cattttatgc tttggcgcat
    28441 aaactttaag tacaagaggt ccacgagcag tgtcaataag aatgacttta gtatcatcaa
    28501 tagaacgaaa aacttttaat gttttaaccc tgcaagttaa aaagtcattt agaacttgct
    28561 cataaaaggg atcattatct tttatgaaaa ctttcaaccc atttatcgtt ttattatata
    28621 tcataatgaa ttattttaac cttaaatctt tagaagcatt tttctttata atactttaaa
    28681 taattaaaaa tgcctgccat ataatgcttt tgtttaaatt tatgttttgc acaataccga
    28741 tactgattgc tatttacagg tttaagtaaa tcttctttac tccacggaga agctgctttg
    28801 gcgatcaaaa aactacgtga aaccggataa tcagcccact catgccaggg ttttgtaggt
    28861 cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt gtcttttaat
    28921 tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac tttaccattc
    28981 aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca atctgggtca
    29041 cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc aatatttatc
    29101 agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa taccgaggct
    29161 cgattttgcc accattcaat atctctttca gctacaactg cggcaatttc attagtagaa
    29221 aattgataat ctaagagttc tttaatacta cccttgcaag caatatctgc atcaagatat
    29281 agtatttttt catgtttatg atagaaataa tctgcaatta taaatcgaaa atatgttgcg
    29341 taagtccagt ttttcgtgct tggtaatgac ttcagcttat cacaattgat aacataaata
    29401 ttaattcttg aattatattg ttttgctaaa tcagaaaaat ataatttgtc tttatcactg
    29461 atataatctg tgaaaacatg aaattcacaa gagatttctc tattgtttaa tagaatagat
    29521 gcgatggcta caccacatcc aaaaagaaaa tttctgtcaa taccaaatgc tatatcaaga
    29581 gtatttttag atcttatatt ttgataatga aaattatact caaaaaggat gatgtcacta
    29641 tcattgagtt gagacatatt tattctgtat ccaaaatttt ggcaaaaata agttacgggt
    29701 tacaacgatt ttcgaatcgt tctttccctg atttttgtgg cttttgcttc tgcttgcgac
    29761 agcagtcctt gttcctgctt gagaatgtct ttaagcgggg cggcaaaata caccttcata
    29821 aagcgccaga tatcccgctg agtcaggcca atattcattg aagaaaaata aagccctata
    29881 agatctttat cgcgccaacg acctggaacg cgcgtgcgaa gctgcgcccg gtgtaggtca
    29941 attaccgaaa tttttaactc ctcttcctta ccggaaaaag gcaagtgcag caggaaatga
    30001 cagatataac agtcacgatg gttaatgccc gcagcatgca tatcgcgcac catcgtcgcg
    30061 acacgcttaa taagcatacg ctttacgcga acatctggtg ggttggtcgc ccagtcagca
    30121 cagtaatctt ccagactgat ggttggtgtc agatcttcgg taataataaa cgaggttctg
    30181 gtcagcggat tcatgccttt ttcgccgaat gccaccccat acatagtatc aacgccgaca
    30241 tcccgcagtc gatgaatcgc attccattcg cggtctgcgc ctaatactgg catccgcaat
    30301 gagagtaaat ttttgattat ctctttcagg gtcgtgccgc gatgccattt gagaaaatag
    30361 ctttttcccg ccatttcaaa gcgcagagta cggcgagttt ccagttcacg aaatacctca
    30421 ccctgcaagg ttttaacttc ctcaaaagga tctttgccgc gccataacgt ggcaaacggc
    30481 tctttaagtt caaccatcca gaccacccgt tatgatatcc gccgctttct ctggcagact
    30541 gtataaatct tgtgtatcag cataatgtcg cgcattttcc gcccaagcct ggcgcaatga
    30601 agattgcgtt aacgctttgc gtaaaatctc attcaatgtt tcctggcgga atggctcagc
    30661 aatagcctcg ccgcaattag cgtcgacaat ataatgcgca tagccacaaa cggcagttgt
    30721 tagtaccggt aatcctgcag taatcgcttc cagcagcaca attcccgccg cttcctggta
    30781 ggcaggatgc agtaataaat ccgccgccgc cattaattcc gagacatcgt tgcgccccga
    30841 gaagaagtga acattactgc gcacgccgcg tttttctgcc agtgcctcaa attttcgcgg
    30901 tttatcctgc ccaacaacat atagcaatgt gttgtggcgc agcgaatccg gtaacgaagc
    30961 aagtgcttca atggaacgat cgacaccttt acgcgtgaag tctgaaccga cctgcaacaa
    31021 taaatattgt tgttcggtta ttccattctt cttacggaag atttcacggc tattggctgg
    31081 ctgctggcta tatttacgat caggataaat ccccggtggc agaatatgaa aacgctccgc
    31141 ttcagtctga taatgtttct ggaaatcggc gatttgctta tctgtcagca tcagcagctg
    31201 tgtcggcttg ccctgttcga aggttgcccg ctcaaaggcg gcataatggc gataacgtga
    31261 cgtcaggcga tagaaaaagc ctttttcctg cgctactttc tcggcataac aaacatcagc
    31321 ggcataataa acgtccagcc ccggcatttt gttgaatcca acgactttat cgacgggatg
    31381 ttcgcgtaaa tgtttttgca cccaggcaaa atactccgca ttgcgcccgt gattggtatg
    31441 cgatttaacc ggcactttga tcagttcaaa tacatcaggg cattcgcctt cccacgactg
    31501 ggtataaacc cgaacatgat gacctcgggc ggcgactgtc tgagcaatac gcataaaatc
    31561 gcgctgcaaa ccgccaaagg gaaaatattt atataaacaa aaagcaacga tcataacgac
    31621 gtacctgtcg tggaggaggg cagtaattta tcgacagcag caatgacatc tgccgccgga
    31681 ataaccgaaa gatacatctc atttcggtca cgctgatcgc gcgttggcat ttcccggtaa
    31741 tctcccgccc agaattgaat catgttattt gaccagggac gccagaaaat atgatctgtt
    31801 gcgccaaaca gcgatatcag cggcgtatta actgcagcgg caatatgcgc cggtgcggaa
    31861 tcaacgccaa taaacagctg cgcatgatcg attaacgcac caagttccgg gaaggtcacc
    31921 tttccagcca gcgccgttac tggtggcgtc tggcatccct gcgcaatttc attgacgcag
    31981 gccagatcgt ctttatctgg gccggacgtc agaacgactt cataaccacg agcatgtaag
    32041 gcatcaatca cagcggaaaa cttggcgttg tcccagcatt tgaagatttg ccgcgccgta
    32101 ggttggataa ccacataatt ttgtccaaca ccagcgtgat caagttcgcg acgcatacgt
    32161 ttccagcttg caggcgggta actcatggtt gtctgcttca ccaacgaatc aagtcccaat
    32221 ggtgtcagca cggataagtt actttccacc acatttccac cctgcaacgg caccaaatgg
    32281 gtgaaacttt tacgccaaaa agcagactgc cgatgatgat aatcctggga aattttcaca
    32341 cgagcattta ataagcgaac cagtatagca accatccatt gatcggtaag attgacgata
    32401 aggtcatact tattggcacg taataccttg atgagatgaa aaaagttggc aattttttct
    32461 gaggcttttg cttttttatt ttttatgccg tagagcgcgt taatctctgg attttcagac
    32521 aggatcggga tggtgtcctg ataaagcagc acatcgattt ttgcgtcagg gtaatttttt
    32581 tt
//
LOCUS       DAJTYI010000032        32554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYI010000032 DAJTYI010000000
VERSION     DAJTYI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1136
                     /gene="waaA"
                     /locus_tag="OO564_001471"
     CDS             <1..1136
                     /gene="waaA"
                     /locus_tag="OO564_001471"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6652645.1"
                     /translation="GGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSER
                     VQSAFGKDVQHVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVI
                     ANARLSARSAAGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLK
                     FDISVTPQLAAKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLIL
                     VPRHPERFPDAINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFV
                     GGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVS
                     SLLTDAVYRSFYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1144..1623
                     /gene="coaD"
                     /locus_tag="OO564_001472"
     CDS             1144..1623
                     /gene="coaD"
                     /locus_tag="OO564_001472"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6652646.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1662..2471)
                     /gene="mutM"
                     /locus_tag="OO564_001473"
     CDS             complement(1662..2471)
                     /gene="mutM"
                     /locus_tag="OO564_001473"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6652647.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2569..2736)
                     /gene="rpmG"
                     /locus_tag="OO564_001474"
     CDS             complement(2569..2736)
                     /gene="rpmG"
                     /locus_tag="OO564_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6652648.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2757..2993)
                     /gene="rpmB"
                     /locus_tag="OO564_001475"
     CDS             complement(2757..2993)
                     /gene="rpmB"
                     /locus_tag="OO564_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6652649.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3210..3878)
                     /gene="radC"
                     /locus_tag="OO564_001476"
                     /pseudo
     CDS             complement(3210..3878)
                     /gene="radC"
                     /locus_tag="OO564_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4050..5270
                     /gene="coaBC"
                     /locus_tag="OO564_001477"
     CDS             4050..5270
                     /gene="coaBC"
                     /locus_tag="OO564_001477"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6652650.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5248..5706
                     /gene="dut"
                     /locus_tag="OO564_001478"
     CDS             5248..5706
                     /gene="dut"
                     /locus_tag="OO564_001478"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6652651.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5813..6409
                     /gene="slmA"
                     /locus_tag="OO564_001479"
     CDS             5813..6409
                     /gene="slmA"
                     /locus_tag="OO564_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6652652.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6446..7087)
                     /gene="pyrE"
                     /locus_tag="OO564_001480"
     CDS             complement(6446..7087)
                     /gene="pyrE"
                     /locus_tag="OO564_001480"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6652653.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7153..7869)
                     /gene="rph"
                     /locus_tag="OO564_001481"
     CDS             complement(7153..7869)
                     /gene="rph"
                     /locus_tag="OO564_001481"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6652654.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            7996..8859
                     /locus_tag="OO564_001482"
     CDS             7996..8859
                     /locus_tag="OO564_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6652655.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9080..9904
                     /gene="dinD"
                     /locus_tag="OO564_001483"
     CDS             9080..9904
                     /gene="dinD"
                     /locus_tag="OO564_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6652656.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10195..10812
                     /locus_tag="OO564_001484"
     CDS             10195..10812
                     /locus_tag="OO564_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6652657.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10809..12326)
                     /gene="ligB"
                     /locus_tag="OO564_001485"
     CDS             complement(10809..12326)
                     /gene="ligB"
                     /locus_tag="OO564_001485"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6652658.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12348..12605)
                     /locus_tag="OO564_001486"
     CDS             complement(12348..12605)
                     /locus_tag="OO564_001486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652659.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12745..13368
                     /gene="gmk"
                     /locus_tag="OO564_001487"
     CDS             12745..13368
                     /gene="gmk"
                     /locus_tag="OO564_001487"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6652660.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13423..13698
                     /gene="rpoZ"
                     /locus_tag="OO564_001488"
     CDS             13423..13698
                     /gene="rpoZ"
                     /locus_tag="OO564_001488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6652661.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13717..15825
                     /gene="spoT"
                     /locus_tag="OO564_001489"
     CDS             13717..15825
                     /gene="spoT"
                     /locus_tag="OO564_001489"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6652662.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15832..16521
                     /gene="trmH"
                     /locus_tag="OO564_001490"
     CDS             15832..16521
                     /gene="trmH"
                     /locus_tag="OO564_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6652663.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16527..18608
                     /gene="recG"
                     /locus_tag="OO564_001491"
     CDS             16527..18608
                     /gene="recG"
                     /locus_tag="OO564_001491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6652664.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18642..19847)
                     /gene="gltS"
                     /locus_tag="OO564_001492"
     CDS             complement(18642..19847)
                     /gene="gltS"
                     /locus_tag="OO564_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6652665.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20127..21518
                     /gene="xanP"
                     /locus_tag="OO564_001493"
     CDS             20127..21518
                     /gene="xanP"
                     /locus_tag="OO564_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6652666.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21639..23348
                     /locus_tag="OO564_001494"
     CDS             21639..23348
                     /locus_tag="OO564_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6652667.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23401..25719)
                     /gene="yicI"
                     /locus_tag="OO564_001495"
     CDS             complement(23401..25719)
                     /gene="yicI"
                     /locus_tag="OO564_001495"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6652668.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25729..27112)
                     /locus_tag="OO564_001496"
                     /pseudo
     CDS             complement(25729..27112)
                     /locus_tag="OO564_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27405..27499
                     /locus_tag="OO564_001497"
     tRNA            27405..27499
                     /locus_tag="OO564_001497"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27439..27441,aa:Sec,seq:tca)
     gene            27799..28983
                     /locus_tag="OO564_001498"
     CDS             27799..28983
                     /locus_tag="OO564_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6652669.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29395..30438
                     /locus_tag="OO564_001499"
     CDS             29395..30438
                     /locus_tag="OO564_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6652670.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            30953..31381
                     /locus_tag="OO564_001500"
                     /pseudo
     CDS             30953..31381
                     /locus_tag="OO564_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31411..32544)
                     /locus_tag="OO564_001501"
                     /pseudo
     CDS             complement(31411..32544)
                     /locus_tag="OO564_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8118 a         8598 c         8093 g         7745 t
ORIGIN      
        1 caggcggcat tatgctgcac tccgtctccg tcggtgaaac tctggcggcg atcccgttgg
       61 tgcgcgcgct gcgtcatcgt tatcctgatt taccgattac cgttacaacc atgacgccaa
      121 ccggttcgga gcgcgtacaa tcggctttcg ggaaggatgt tcagcacgtt tatctgccgt
      181 atgacctgcc cgatgcgctc aaccgtttcc tgaataaagt cgaccccaaa ctggtgttga
      241 ttatggaaac cgaactatgg cctaacctga ttgcggcgct acataaacgt aaaattccgc
      301 tggtgatcgc taacgcgcga ctctctgccc gctcggccgc aggttatgcc aaactgggta
      361 aattcgtccg ccgcttgctg cgtcgtatta cgctgattgc tgcgcaaaat gaagaagatg
      421 gtgcacgttt tgtggcgctg ggcgcaaaaa ataaccaggt aaccgttacc ggtagcctga
      481 aattcgatat ttctgtaact ccgcagttgg ctgctaaagc cgtgacgcta cgccgccaat
      541 gggcaccaca ccgcccggta tggattgcca ccagcactca cgaaggtgaa gagagcgtgg
      601 tcattgccgc acatcaggca ttgttacagc aattcccgaa tttattgctc atcctggtac
      661 cccgtcatcc agaacgcttc ccggatgcga ttaaccttgt ccgccaggct ggactaagct
      721 atatcacacg ctcttcaggg gaagtcccct ccaccagcac gcaggttgtg gttggcgata
      781 cgatgggcga gttgatgtta ctgtacggca ttgccgatct cgcctttgtt ggcggttcac
      841 tggttgaacg tggtgggcat aatccgctgg aagctgccgc acacgctatt ccggtattga
      901 tggggccgca tacttttaac tttaaagaca tttgcgcgcg gctggagcag gcaagcgggc
      961 ttattaccgt taccgatgcc actacgcttg caaaagaggt ttcctcttta ctcaccgacg
     1021 ccgtttaccg tagtttctat ggccgtcatg ccgttgaagt actgtatcaa aaccagggcg
     1081 cgctacagcg tctgcttcaa ctgctggaac cttacctgcc accgaaaacg cattgaggtt
     1141 gttatgcaaa aacgggcgat ttatccgggt actttcgatc ccattaccaa tggtcatatc
     1201 gatatcgtga cgcgcgccac gcagatgttc gatcacgtta ttctggcgat tgccgccagc
     1261 cccagtaaaa aaccgatgtt taccctggaa gagcgtgtgg cactggcaca gcaggcaacc
     1321 gcgcatctgg ggaacgtgga agtggtcggg tttagtgatt tgatggcgaa cttcgcccgt
     1381 aatcaacacg ctacggtgct gattcgtggc ctgcgtgcgg tggcggattt tgaatatgaa
     1441 atgcagctgg cgcatatgaa tcgccactta atgccggaac tggaaagtgt gtttctgatg
     1501 ccgtcgaaag agtggtcgtt tatctcttca tcgttggtga aagaggtggc gcgccatcag
     1561 ggcgatgtca cccatttcct gccggagaat gtccatcagg cgctgatggc aaaattagcg
     1621 tagcgtttat gccggatggt atgccatccg gctcgcatga attacttctg gcactgccga
     1681 caataaaacg ttgcccgctg cgcatgttta gtcgccacaa tcggcgtacc gcacacccga
     1741 cacggctcac cttttcgccc gtaaacctgc aattcctgcg cgaaatagcc cggtttacca
     1801 tcactttgca gaaaatcttt cagcgttgta ccaccctgct caatcgaacg cagcaacacc
     1861 gctttaatca cccgagctaa caattcacac tctgccagcg acagtgatga cgccagccga
     1921 tccggatgga tccccgccgc aaacagtgat tcgctggcat agatattccc tacccctacc
     1981 accagcttgt tatccatcag ccacggttta atcgccgttt ttttcttcgc gcacttctga
     2041 tgcagatact caccattgaa atcgtcgcta agcggctccg gtccaagatg ggccagcaca
     2101 ttatgccctt ccagctcttt ggtccacagc caggcaccaa agcggcgcgg atcggtgtag
     2161 cgcagcactt taccgttgct catcaccaaa tccacatggt catgcttttc agggggaagt
     2221 tcttctggaa ggatgcgcag gctgccagac atccccaaat gaatgataat ccagccctca
     2281 ggcagctcca gcagcagata tttagcccgc cgctgcacgc taagcactgg ttgatcgctt
     2341 aatcggtaga tctcttctga aaccggccag cgcaagcgtc cgttgcgcac caccgcatga
     2401 agaatggttg caccaacgag attcggttct atgccgcgac ggctggtttc aacttcgggt
     2461 aattcaggca tagcatctcc aggaatgaac agatgcaatc aatatggggg caagcagata
     2521 acaaaaaacc ccgccggagc gaggtttttc gttacatcaa agcgagaatt atttgatttt
     2581 cgcttctttg tagatcacgt gctgacgaac aactggatcg aattttttca gttccagttt
     2641 ttccggctta gtacgtttgt tcttcgtagt ggtatagaag tgaccagtac cagcagaaga
     2701 aaccagcttg attttctcac gaataccttt agccatgatt tatttcctct aagtacttag
     2761 tacttttcgc cacgggcacg cagttcagcc agaactgtat cgatgccttt tttatcgatt
     2821 acacgcatac ctttagcaga tacacgcagg gtgacaaaac gcttctcgct ctcaacccag
     2881 aaacggtgag agtgcaggtt cggcaggaaa cggcgtttag tcgcgttcag tgcgtgggaa
     2941 cggttgttac cggtcaccgg acgcttgcca gtaacttggc agactcggga catgtctatt
     3001 ctccaaaaat caaattagct cgagcttcgt atggggtatt ggcgcctcgt caggctttac
     3061 agcccggtca tcgcagttct atgtgaactc tcgattgcca ggcccaaatg ccaaacccga
     3121 gattctcaaa ggtggcgtag tatacgctga ctcagcgatg tgctcaagtc ccgaacagac
     3181 aaagatcccg aaggatcgcg catagcgggt taaatccagc cgcgttcggc aaaagaaaca
     3241 tactctccac gcccaatcac gatatggtcg agcacgcgta aatccatgaa ctgacaactc
     3301 tttattatcc gttcagtaat gagtttatcc gctttactgg gttcagcaca acccgaaggg
     3361 tgattatgtg caaggatcag cgccgaggcg tttattttta tcgcttagcg gataatttcc
     3421 cgaggatgga cttcaacatg gtttagcgtg ccggaaaaaa gacggctatg cgttataacc
     3481 cggtgttgtg agtcgagaaa gatcaccata aagatctccc gctcctcacc cgtgagctgg
     3541 ctttgtaaaa attcccgcgt catctccggg ctgagtaaag ggctttcttc acgcatccgc
     3601 acgttgtagt aacgtcgcgc cagttcagca atccccttta actgggcaaa tttcgctacg
     3661 ccaattccat gaacaccact aaattgttca tattcagagg ttaacaagcc ataaagagag
     3721 ccgaaattct ccagcatctc ttttgccagg gttaatacat ctttaccgcg cgttccggta
     3781 cgcagaaata gcgccagcag ctcgacatcc gttaaggcgc taataccaaa cttcagcatt
     3841 ttttcgcgcg gcatcaacag ctgtgcattg tttttcacct tcacctcctt tgtggtgctc
     3901 gcatcctgtc atagccgttc cgttaaatcg acggccagtt ttcaatcctg gaaagagcct
     3961 cgcaaagtga atctcaggtg aaggcacgac aacaaaagta ttgtgataaa atcgccaact
     4021 tctggtgtca cccaacagga aaaatcatca tgagcctggc cggtaaaaaa atcgttctcg
     4081 gcgttagcgg cggtattgct gcctataaaa cccctgaact ggtgcgtcgt ttgcgcgatc
     4141 gcggggccga cgtccgcgta gccatgaccg aagcggcaaa agcctttatc accccactta
     4201 gcttgcaggc ggtttctggt tatcccgttt ccgacagtct gcttgacccg gcagccgaag
     4261 cagctatggg ccatattgag ctgggtaaat gggctgattt agtgattctc gcccctgcca
     4321 cggcagattt gattgcccgt gttgctgccg gaatggcgaa tgacctggta tcgacgattt
     4381 gtctggctac acctgcgcct gtagccgtgc tccccgccat gaaccagcag atgtaccgtg
     4441 ccgctgccac gcagcataat ttagaggtgc ttgcttcccg tggtttgctc atctgggggc
     4501 cagacagtgg cagtcaggct tgtggtgata tcggtcctgg acgaatgctc gatccgttaa
     4561 ccattgtgga tatggcggtg gcgcattttt cgcccgtcaa cgacctgaaa catctgaaca
     4621 ttatgattac cgccggtccg acgcgtgaac cgctcgatcc ggtgcgttat atctctaatc
     4681 acagctccgg caagatgggt tttgctatcg ccgccgctgc tgcccgtcgt ggcgcgaacg
     4741 tcacgctggt atcaggtcca gtttcactac cgacgccacc gtttgttaaa cgtgttgatg
     4801 tgatgaccgc gctggaaatg gaagccgccg tgaatgcttc tgtacagcag caaaatattt
     4861 ttatcggttg cgccgccgtg gcggattatc gcgcagctac cgtggcccca gaaaaaatca
     4921 aaaagcaggc cacgcagggt gatgaattaa caataaaaat ggttaaaaac cccgatatcg
     4981 tcgcaggcgt tgccgcacta aaagaccatc gaccctacgt cgttggattt gccgccgaaa
     5041 caaataatgt ggaagaatac gcccggcaaa aacgtatccg taaaaacctt gatctgatct
     5101 gcgcgaacga tgtttcccag ccaactcaag gatttaacag cgacaacaac gcattacacc
     5161 ttttctggca ggacggagat aaagtcttac cgcttgagcg caaagagctc cttggccaat
     5221 tattactcga cgagatcgtg acccgttatg atgaaaaaaa tcgacgttaa gattctggac
     5281 ccgcgcgttg ggaaggaatt tccgctcccg acttatgcca cctctggctc tgccggactt
     5341 gacctgcgtg cctgtctcga cgacgccgta gaactggctc cgggtgacac tacgctggtt
     5401 ccgaccgggc tggcgattca tattgccgat ccttcactgg cggcaatgat gctgccgcgc
     5461 tccggattgg gacataagca cggtatcgtg cttggtaacc tggtaggatt gatcgattcc
     5521 gactatcagg gccagttgat gatttccgtg tggaaccgtg gtcaggacag cttcaccatt
     5581 caacctggcg aacgcatcgc ccagatgatt tttgttccgg tagtacaggc tgaatttaat
     5641 ctggtggaag atttcgacgc caccgaccgc ggtgaaggcg gctttggtca ctctggtcgt
     5701 cagtaacaca tacgcatccg aataacgtca taacatagcc gcaaacattt cgtttgcggt
     5761 catagcgtgg gtgccgcctg gcaagtgctt attttcaggg gtattttgta acatggcaga
     5821 aaaacaaact gcgaaaagga accgtcgcga ggaaatactt cagtctctgg cgctgatgct
     5881 ggaatccagc gatggaagcc aacgtatcac gacggcaaaa ctggccgcct ctgtcggcgt
     5941 ttccgaagcg gcactgtatc gccacttccc cagtaagacc cgcatgttcg atagcctgat
     6001 tgagtttatc gaagatagcc tgattactcg catcaacctg attctgaaag atgagaaaga
     6061 caccacagcg cgcctgcgtc tgattgtgtt gctgcttctc ggttttggtg agcgtaatcc
     6121 tggcctgacc cgcatcctca ctggtcatgc gctaatgttt gaacaggatc gcctgcaagg
     6181 gcgcatcaac cagctgttcg agcgtattga agcgcagctg cgccaggtat tgcgtgaaaa
     6241 gagaatgcgt gagggtgaag gttacaccac cgatgaaacc ctgctggcaa gccagatcct
     6301 ggccttctgt gaaggtatgc tgtcacgttt tgtccgcagc gaatttaaat accgcccgac
     6361 ggatgatttt gacgcccgct ggccgctaat tgccgcgcag ttgcagtaat atgacgccgg
     6421 atgacttttc atccggcgag tttctttaaa cgccaaactc ttcgcgatag gccttaaccg
     6481 ccgccagatg ttccgccatt tccggcttct cttccagata ggcaatcaga tctttcaggg
     6541 tgatgataga gatcactttg cagttgtaat cacgctcaac ttcctgaatc gccgaaatct
     6601 cgccgcgccc gcgttcctga cgatcgagcg aaatcaacac gccagcaagc gtcgcgccat
     6661 tggcctgaat aatctccatc gactcgcgaa tcgccgttcc ggcggtgatc acatcatcta
     6721 ccagcattac gcgtccttgt aacgcgctac caaccagatt gccgccttca ccgtggtctt
     6781 ttgcttcttt gcggttaaag cagtacggca ggtccaggtc gtgatgctcc gccagtgcca
     6841 cagcggttgt ggtggcaatc gggatccctt tgtaagcagg gccaaacagc agatcgaact
     6901 caatgccgga atccaccaac gcttcagcgt aaaaacggcc taacagtgcc agatcgcgcc
     6961 cggtattaaa cagcccggcg ttgaagaaat aggggctttt gcgcccggat ttcagcgtaa
     7021 actcgccaaa ctttaacacc tgcttgctaa gcgcaaattc aataaactgg cgctgatatg
     7081 gtttcatgcc ttcgctcctc atcttacttt tctacagaca aaaaaaaggc gactcatcag
     7141 tcgccttaaa aatcagtttg ccagcgccgc cttctgcgtc gctacaatgg attcgattcc
     7201 ccctcgggcc agcgccaaca aggtgagtag ctcttcatgg gtgaacggct cgccttctgc
     7261 cgtcccctgc acttcaatga tgcgcccgtc ttcggtcatc actacgttca tgtcggtctc
     7321 tgcggctgag tcttcaacgt attccagatc gcaaattgct tcaccgttta caataccgac
     7381 agaaactgcg gctaccatcc ctttcatcgg gttggttttc agcttgccgt tttccaccag
     7441 cttctgtagc gcatctgcca gcgccacgca ggcacccgta atcgacgcgg tacgagtgcc
     7501 accatcagcc tgaagcacgt cgcagtccag cgtaatggtg aactcaccca gcgctttcaa
     7561 atctactgcc gcgcgaagag cacgggcgat cagacgctgg atttccattg tgcgtccacc
     7621 ctgcttacct ttcgccgctt cacgagcgtt acgggtgtgg gtagaacgtg gcagcatgcc
     7681 gtactctgcg gtgatccagc cctggccctg acctttcagg aagcgcggca cgccttcttc
     7741 aatagaggcg gtacacaata ctttggtatc gccaaattcg accagcaccg agccttctgc
     7801 atgttttgta tagttacgag tcagggtaac gggacgcacc tgattattgc tacggcctgc
     7861 tggacgcata ttgaaatctc cggcttgaaa caaatgtggc tgcgcattat acggacttcc
     7921 ggcggttatt cctatcctga caaggcatcg atggctataa tccttccacc tctcctttta
     7981 taaacaggaa cgtctatgat ccgcagtatg accgcctacg cccggcgtga aatcaagggt
     8041 gaatggggga gcgcaacctg ggaaatgcgc tcggtaaacc agcgttatct ggaaacttac
     8101 tttcgtctgc cggagcagtt ccgtagcctt gaacctgtcg ttcgcgagcg tattcgttct
     8161 cgcctgacgc gcggtaaagt ggaatgtacc ctgcgctatg agccagatgt tagcgcacaa
     8221 ggtgagctga tcctcaatga aaaactggct aaacagctgg taactgccgc gaactgggta
     8281 aaaatgcaga gtgacgaagg ggaaatcaac ccggttgata ttctacgctg gccgggcgtg
     8341 atggcagccc aggagcagga tctcgacgcg attgccgctg aaattctcgc ggcgttggat
     8401 ggtacgctgg acgactttat cgtcgcgcgc gaaaccgaag gtcaggcact gaaagcattg
     8461 atcgagcagc gtctggaagg cgtcaccgcc gaagtggtca aagtccgcgc ccatatgccg
     8521 gaaattctgc aatggcagcg tgagcgtctg gtcgcgaagc tggaagatgc tcaggttcag
     8581 ttggaaaaca accgtctgga gcaggagttg gttctgctgg cacaacgaat tgacgttgcc
     8641 gaagagctgg accgcctcga agcgcatgtt aaagagacgt acaacattct gaagaaaaaa
     8701 gaagcggttg gtcgtcgtct ggactttatg atgcaggagt tcaaccgtga gtcgaacact
     8761 cttgcgtcga aatctatcaa tgccgaagtg acaaactccg ccatcgagct gaaagtgttg
     8821 attgagcaga tgcgcgagca gattcagaat attgagtaac tcgtaacacc aattctgact
     8881 tttcagcctt cacaaatgcc gcccactcaa acagagcggc atttttcttc cccgcaactt
     8941 caattctgtt ttgcgtgcct gctccagatt ttgcgatgtt tttttgccca gcacactgag
     9001 aacgtgagat actcacaact gtatataaat acagttacag atttactttc tttgcaatcg
     9061 atatcacatg gagtgggcaa tgaacgaaca tcatcaacct tttgaagaga taaaactgat
     9121 taatgcaaac ggagcagaac aatggtcagc aagacaactt gggaaactac tgggttattc
     9181 agagtatcgt cactttatac ctgtattaac gcgcgccaaa gaggcctgtg agaacagtgg
     9241 tcacacaatt gatgaccatt tcgaggagat cctcgatatg gtcaaaattg gctcaaatgc
     9301 caaaagagca ttaaaagaca tcgtactctc ccgctatgcc tgttacctgg tagtacaaaa
     9361 cggcgacccc gcgaaaccgg tcattgcggc agggcagact tattttacta tccagacccg
     9421 acggcaggag cttgctgacg atgaagcatt taagcaactt cgtgaagacg aaaagcgtct
     9481 gtttctgaga aatgagttga aagaacataa caaacaattg gttgaggcag cacagcaagc
     9541 tggggtagcg acagctactg atttcgccat atttcagaat catggttacc aggggctgta
     9601 tggtggatta gatcagaaag ccatccacca actgaagggg ctgaaaaaga gtcagaagat
     9661 cctggatcat atgggttcaa cagaactggc ggctaatctc tttcgagcta cccaaacaga
     9721 agaaaaactc aagcgggata gcgtgaattc aaaacagcaa gctaatacca cacactttga
     9781 cgtgggtagc aaggtgaggc aaaccattca ggaacttggc ggaaccatgc ctgaggagtt
     9841 gccgaccccg caggtcagca tcaagcagtt ggaaaacagt gtaaaaatta cagagaagaa
     9901 atagcagaaa cgaaaaactc tttttgttga ttgagacacc cgatgcgtaa gccaaggtcc
     9961 aggtgcagtg aacacaatgg ctaaacattg cacctttctt ttcccccctc agttttaacc
    10021 tattttttct tatgcatttt ctcagacaag aagtcagaag aatgcatcta cgctacagaa
    10081 aatagcgatt tcacataact aaaagttatc aatttcctct caccttaaaa aatatcaatc
    10141 gtgacaatgc gcacaaatcg ctaccctgcc agacagattt ttagggagag aaccatgctg
    10201 ttacacattt tgtatttggt tggcattact gccgaagcca tgacgggggc gctggcggcc
    10261 gggcgacggc gcatggatac atttggcgta attattattg caaccgccac cgcaattggc
    10321 ggagggtcag tgcgcgatat tctgctgggc cactatccgc tcggctgggt caaacacccg
    10381 gaatatgtga ttatcgtcgc taccgccgca gtactcacca ctatcgttgc tcctgtaatg
    10441 ccttacctgc gcaaagtgtt tctggtgctc gatgcgctcg gactggtggt cttttctatc
    10501 atcggcgcac aggttgcact ggatatgggg cacggcccaa ttattgccgt tgtcgcggcg
    10561 gtaaccaccg gtgtatttgg cggcgttttg cgcgatatgt tctgtaaacg cattccgctg
    10621 gtctttcaaa aagagttgta tgccggtgtc tcatttgcct ccgccgtgct gtacatcgcg
    10681 ctgcaacact atgtttctaa ccatgatgtg gtgattatct ccaccctggt attcggcttt
    10741 tttgctcgtt tactggcgct acgtcttaag ctgggattac cggtctttta ctacagccac
    10801 gaaggtcact aaggttcaaa acctgtgatc tgctgggcag ccagccaact gcccagcttc
    10861 ttgatttgcg cattttcctt ccattcaata acctgtctgg cgcgcccaga tccagtcccc
    10921 ggcagctgct gccagaactg ctccgtgcta aataaaagtt gcgaccagga tcgttcatca
    10981 ctggcattaa gcgccgcccg ggttagcggt attcccattg ccatcaccca gcgagtaaaa
    11041 ggctggttac gagccagatt aaactgatgc cataactgcg cacttttact tttcgcgatc
    11101 cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc aggaaaagat atgctcaaag
    11161 cgatgagtct gatgcagcgc gcgccaaccg gcctcaccaa tgccatccag ccccagaacc
    11221 tgttttgacc ccagccagac taagcgtgaa atgaactgtt cctgacaaac atcagaagca
    11281 aagtagcagg tcaacgagtt aaagcggttt tctggtggtg tcggttttgt acgttctgca
    11341 ccgcgccaga ccacatcatc aatgcgagga ataccctgac cgccaaggct gacgaggatc
    11401 tgatcaccag gcgcaatatc ccactcctgc cagcgcctga cggaaccaat attcacccgc
    11461 tggacttttt tatcatccag catgacaggt gcgagtgacg caaccaccga tattttaccg
    11521 ctcttaccca ccgcaaactg aatcgccttc acttcggcaa cctgagctac aggttgatat
    11581 ttccaggcca ccagccactc cgcctggccc ggtagccaat gacgggattc gggttcctta
    11641 gcccctcgca caaccacgcc atcggtgacg aagggtaatt tcgctttcca ccactcattg
    11701 cgtacgcgcg caacctcatc agcattttta accgcacggg tatacctctg cgttagagta
    11761 aaacctgcgg tagccagctc ctttaaacga tcggtcatta actgcggtcc atccggccat
    11821 gcccagacaa aaacagccag tgagtttagc gtgtcgctat tcccctggcg catcatcagg
    11881 ccagcaactt ttgcgcgggc atttattccc cccatttgtt gttggatatg cccctcgcgc
    11941 tggagaaata tttccccctg aagcgtactg ttggctaaag gcccgctaac ggtttgcggc
    12001 acagcggaaa ttaagctaac tttctgcgtc cagtcctcgc ctttcaggcc attaccgcga
    12061 ctgattgctt tgttcagttt cccgtcccga taaaccaggg ttaccgcaac gccatcaact
    12121 tttggctgca cccaaagatc actacgttct cgcatccaca gacttaatgc gtttttatcc
    12181 gccattttgc gtacacccgt atgggcaacc ggatgcataa ccgcaccatt taaaggcggc
    12241 atcatcacgt cgcggggctc gctcacaaag cagcgttgcc actgcgtaag acgggcgctt
    12301 aactgatcgt aaacaccgtc ttccacctca ctttttcctt ccagtagtca tcgtcccact
    12361 gttttatttg ctgttgcagg cgggaaattt cttcctgtgc tctggctggc gaccaggccg
    12421 gacagaccgc ccacacagat gattgccagc acaagatact tattaatatc gccatccata
    12481 ctttcatctt cacctccgct gtagtcaggc tggcagatat acaacgtggt gaaggcaaag
    12541 ccgagtggca aaaacggagt ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa
    12601 atcacaggca aatgcggaaa cagctacgca aaacgcaaca actttgcgca aaaagtgtga
    12661 gcaagggcta cgtcacatgg ccgcgccgtg tataataagc tcgtatgtag gctttatttc
    12721 gctaatcaca tacgaaagat actcatggct caaggcacgc tttatattgt ttctgccccc
    12781 agtggcgcgg gtaaatccag cctgattcag gctttattaa aaacccaacc gttgtatgac
    12841 acccaggttt ctgtttcaca caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa
    12901 cattatttct ttgttaatca tgatgaattt aaagaaatga ttagcagaga tgcgttcctc
    12961 gaacacgcag aagtttttgg taattactat ggcacttcgc gtgaggccat tgagcaagta
    13021 ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc agggcgcgca gcaaattcgc
    13081 cagaagatgc cgcacgcgcg gagtatcttt attttaccgc cgtccaaaat tgaactggac
    13141 cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa
    13201 gctgttgcag aaatgagcca ttacgccgaa tatgattatc tgattgtgaa tgatgacttc
    13261 gataccgcgt tgaccgattt gaagaccatt attcgcgccg aacgtctgcg catgagccgc
    13321 caaaagcagc gtcatgacgc tttaatcagc aaattgttgg cagactgaac ctgatttcag
    13381 tatcatgccc agtcatttct tcacctgtgg agctttttaa gtatggcacg cgtaactgtt
    13441 caggacgctg tagagaaaat tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc
    13501 gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa cgataaaacc
    13561 actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca acaaccagat cctcgacgtt
    13621 cgcgaacgcc aggaacagca agagcaggaa gccgctgaat tacaagccgt taccgctatt
    13681 gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt atctgtttga aagcctgaat
    13741 caactgattc aaacctacct gccggaagac caaatcaagc gtctgcggca ggcgtatctc
    13801 gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac
    13861 ccggtagcgg ttgcctgcat tctggccgag atgaaactcg actatgaaac gctgatggcg
    13921 gcgctgctgc atgacgtgat tgaagatact cccgccacct accaggatat ggaacagctt
    13981 tttggtaaaa gcgtcgccga gctggtagag ggggtgtcga aacttgataa actcaagttc
    14041 cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga tgattatggc gatggtgcag
    14101 gatatccgcg tcatcctcat caaacttgcc gaccgtaccc acaacatgcg cacgctgggc
    14161 tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg
    14221 ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac tcgaagagct gggttttgag
    14281 gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac
    14341 cgtaaagaga tgatccaaaa aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga
    14401 attccctgcc gcgtcagtgg tcgcgagaag catctttatt cgatttactg caaaatggtg
    14461 ctcaaagagc agcgttttca ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat
    14521 gattccgaca cctgttatcg cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg
    14581 ggtcgcgtga aagactacat cgccattcca aaagcgaacg gctatcagtc tttgcacacc
    14641 tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga tccgtaccga agatatggat
    14701 cagatggcgg agatgggtgt tgccgcgcac tgggcttata aagagcacgg cgaaaccagc
    14761 accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc tgctggagct gcaacagagc
    14821 gccggtagtt cgtttgaatt tatcgagagc gttaaatccg atctcttccc ggatgagatt
    14881 tacgttttca caccggaagg gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac
    14941 ttcgcttatg cagtgcatac cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc
    15001 cagccttacc cgctgtcgca gccgcttacc agcggtcaaa ccgttgaaat cattaccgca
    15061 ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc
    15121 aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt
    15181 ctgctcaacc atgctttggg tggtagccgt aagctgaatg aaatcccgca ggaaaatatt
    15241 cagcgcgagc tggatcgcat gaagctggca acgcttgacg atctgctggc agaaatcgga
    15301 cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt
    15361 ccaccggcaa cccaaagcca cggacatctg cccattaaag gtgccgatgg cgtgctgatc
    15421 acctttgcga aatgctgccg ccctattcct ggcgacccga ttatcgccca cgtcagcccc
    15481 ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata tccgtggcta ccagaaagag
    15541 ccagagaagt ttatggctgt ggaatgggat aaagagacgg cgcaggagtt catcaccgaa
    15601 atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa acctgacggc ggcaattaac
    15661 accacgactt cgaatattca aagtttgaat acggaagaga aagatggtcg cgtctacagc
    15721 gcctttattc gtctgaccgc tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc
    15781 cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa attaatgttt tatgaaccca
    15841 acacgttatg cacgcatctg cgaaatgctc gccaggcggc agcctgatct gaccgtctgc
    15901 atggagcagg tccacaaacc tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt
    15961 ggcgtacatg aagttcacgc cgtctggcct ggtagccgca tgcgcaccat ggcttcggca
    16021 gcggcgggta gtaacagctg ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc
    16081 gctcatctca aaggccaggg catgcagatt ctggcaaccc atctttctga taatgctgtc
    16141 gatttccgcg aaattgatta cactcgcccg acctgcattt tgatgggaca ggagaaaacg
    16201 ggcatcacgc aggaagcatt ggccctggcg gatcaggaca tcatcattcc gatgatcggc
    16261 atggtgcagt cgctgaatgt ttccgttgcc tcagccctca ttctttacga agcccagcgt
    16321 cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca tgttgccgga agcagagcaa
    16381 caacgcctgt tgtttgaagg cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc
    16441 ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag ctgatgccga ctggtgggct
    16501 actatgcagg ctgcagggta agtgccatga aaggtcgcct gttagatgct gtcccactca
    16561 gttccctaac gggcgttggc gcagcactta gtaacaaact ggcgaaaatc aacctgcata
    16621 ccgtgcagga tctactctta caccttcccc tgcgctacga agatcgcacc catctctacc
    16681 ccatcggaga actactgccg ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca
    16741 atatctcctt cggcggtcgg cggatgatga cctgccagat cagcgacggt tccggcatcc
    16801 tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc
    16861 gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg
    16921 aataccgcgt gcagggcgat ctcagcacgc cagaattaca ggaaacgctc acgccggttt
    16981 atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa attaaccgac caggcgctgg
    17041 atctgctcga cacctgcgcc attgaagaac tcctgccgcc ggaactgtca caaggaatga
    17101 tgacgctacc ggaagcgttg cgcactttgc accgcccgcc accgacgcta cagcttagcg
    17161 atctggaaac cgggcagcat ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc
    17221 acaacctcag catgttagcc ttacgtgccg gagcacagcg ttttcatgcc cagccgctga
    17281 gcgccaatga cacgctgaaa aataaactcc tcgccgcctt accgttcaag ccaacgggcg
    17341 cacaggcacg cgtagtggcg gagatcgagc atgatatggc gctggatgtg ccgatgatgc
    17401 gcctggtaca gggcgatgta ggttccggta aaacgctggt cgccgccctc gccgcgctgc
    17461 gtgcgattgc ccacggcaaa caggtggcac tgatggcacc aaccgaatta ctcgccgagc
    17521 agcacgccaa taacttccgc aactggtttg caccgctcgg tatcgaagtg ggctggctcg
    17581 ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga agccatcgcc agcggtcagg
    17641 tgcagatgat tgtcggtaca cacgccatct tccaggaaca ggtgcagttt aacggcctgg
    17701 cgctggtgat tatcgacgaa cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg
    17761 agaaaggcca gcagcagggc ttccatccgc atcagttgat catgaccgcc acgccgatcc
    17821 cccgcacgct ggcaatgacg gcgtatgccg atctcgatac ctcggtgata gatgagctgc
    17881 cgccaggccg cacgccagtg actacggtcg ctattcctga tacccgccgt accgacatca
    17941 ttgaccgcgt gcgccacgcc tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt
    18001 tgattgaaga gtcagagtta ctggaagcgc aggcggcgga agctacctgg gaagagttga
    18061 aactggcgct accagaattg aatgttggcc tggtacacgg gcggatgaaa ccagccgaga
    18121 aacaggcggt gatggcgtcg tttaaacaag gtgagctaca tctgctggtt gccactaccg
    18181 ttattgaagt cggtgttgat gtgcctaacg ccagcctgat gattatcgaa aaccctgagc
    18241 gtctgggtct ggcgcagtta caccagctgc gtgggcgcgt aggtcgtggc gcggtggctt
    18301 ctcactgcgt gctgctctac aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag
    18361 tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa agatctggag attcgcggcc
    18421 ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga atttaaagtg gcggatttac
    18481 tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc gcgacatatt cacgaacgtt
    18541 acccacaaca ggcaaaagcc ttgatagaac gctggatgcc ggagacagaa cgttactcga
    18601 atgcgtaaaa ggcggcggtg agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc
    18661 aacattaaat acagcttaat caccagcgca ttgacgatat cgataaagaa cgcaccgacc
    18721 atcggcacca ccaaaaacgc catgtgcgac gggccaaagc gttcagtgat cgcctgcatg
    18781 ttggcgattg ccgttggcgt tgcaccgagg ccaaaaccac agtgacccgc agccagcact
    18841 gccgcatcgt agtttttgcc catcatgcgc caggtaacga agatggcata caacgccatg
    18901 aagatggtct gtaccaccag aatcgccagc atcggcagtg ccagcgaagc cagctcccac
    18961 agtttcagcc ccatcaacgc catcgccagg aacaacgaca agcttacgtt acccagcacg
    19021 gataccgcac gctcaaagac gcggtaaaag cccattattg acagaccgtt gctcagaatc
    19081 acgccaacaa acagtacaca gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt
    19141 tgcgcaacaa ttttccccac cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc
    19201 accaacgagg tgatcatgcg tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg
    19261 tcatccggaa taccgttcgg cgtggtggag tgtttcacca gatagcgcgc caccggaccg
    19321 ccaatcaagc cgcccagcac cagaccgaac gttgcacagg ccatcgccac ttccgtcgca
    19381 ttggtgaagc cataacgttc aatgaacaat ttactccacg cagcgcccgt accgtgaccg
    19441 ccggaaagcg taatagaacc ggccaacagc cccatcagcg gatcaagccc taacaggcta
    19501 gccataccaa tgccaatggc attttgcatc accaacagac caacaaccac aatcaagaag
    19561 atgccaacca cacgcccacc ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg
    19621 gcgaagaaag ccagcattaa cggatcgcgc agggacatat caaagttgac ttcccagccc
    19681 atgctttttt tcagtactag tagcgccagc gccaccaaca aaccacccgc aacaggttcc
    19741 ggtatggtgt atttcttcaa aaaggagacg gaatggacca acttacgccc gagcagcaac
    19801 gtcagcgttg cggcaacaag cgttgctaaa gtatcgagat gaaacatagt tactcctttg
    19861 tatccgcatc actctttcat actcgtcata cttcatcgcg cagtggcaac ttcatgaact
    19921 atgacgggta tcgagttatg ctttcctggg tcaaattctc agctgttaat gaacaaatgg
    19981 catggatttt aagcagaaaa ataccaaaag ttatataaaa aagtgcattt atctcattta
    20041 aatatttaga tgaggatgtg gcaaacgttt gcttttctct ttcggtcagc taaaatgccc
    20101 gctttgctac cacgggattg ttttcaatgt ctgtttccac cctcgagtca gaaaatgcgc
    20161 aaccggttgc gcagactcaa aacagcgaac tgatttaccg tcttgaagat cgtccgccgc
    20221 ttcctcaaac cctgtttgcc gcctgtcagc atctgctggc gatgttcgtt gcggtgatca
    20281 cgccagcgct attaatctgc caggcgctgg gtttaccggc acaagacacg caacacatta
    20341 ttagtatgtc gctgtttgcc tccggtgtgg catcgattat tcaaattaag gcctggggtc
    20401 cggttggctc cgggctgttg tctattcagg gcaccagctt caactttgtt gccccgctga
    20461 ttatgggcgg taccgcgctg aaaaccggtg gtgctgatgt tcctaccatg atggcggctt
    20521 tgttcggcac gttgatgctg gcaagttgca ccgagatggt gatctcccgc gttctgcatc
    20581 tggcgcgccg cattattacg ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt
    20641 cgctaattca ggtggggctg acctccattg gcggcggtta cgcagccatg agcgataaca
    20701 ccttcggcgc accgaaaaat ctgctgctgg caggcgtggt tttagcctta attatcctgc
    20761 ttaaccgtca acgtaaccct tacttacgcg tggcctcact ggtaattgcg atggcggccg
    20821 gatatgcgct ggcgtggttt atgggcatgt tgccagaaag caacgaaccg atgacgcaag
    20881 aactgattat ggtgccaacg ccgctctatt acggtcttgg cattgaatgg agtctgctgc
    20941 tgccgctgat gctggtcttt atgatcactt cgctggaaac cattggcgat atcacggcga
    21001 cctctgacgt ttccgaacag ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg
    21061 gcgtgctggc aaacggcctg aactcgtttg tttcggcggt gtttaacacc ttcccgaact
    21121 cctgcttcgg gcaaaacaac ggagtgatcc agttgactgg tgttgccagc cgctatgtcg
    21181 gttttgtcgt cgcgctgatg ttgatcgtgc tgggtctgtt cccggcagtg agcgattttg
    21241 tacaacacat tccagaaccg gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg
    21301 ccgcctccgg tgtgcgtatc gtttctcgtg agccgctgaa ccgtcgggcg attctgatta
    21361 tcgcgttgtc gctggcggtt ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg
    21421 cccctgaatg gctgaaaaac ctgctctcct ccgggatcgc cgcgggcggt attactgcca
    21481 tcgtgctgaa tctgattttc ccaccagaaa aacagtaatt cactggcggc ggtaagataa
    21541 ttaccgccgc agtctcacct ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg
    21601 cataactccc ttatgtgcat ttcacgggat ggaagaccat gaaatttatt gggaagctgc
    21661 ttctctacat tctcatcgct ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa
    21721 cccgctgggg agcagaacat atcagcgcat gggtttccga gaatagcgac tatcatctgg
    21781 ccttcggggc gatggatcac cgtttttccg cgccatctca tatcgtgctg gagaatgtca
    21841 cgtttggtcg tgatggtcag cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa
    21901 gcagtcgcca actgaccgaa ccacgccatg tcgataccat cctgctggaa aacgggacgc
    21961 tgaatctcac cgaccagacc gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg
    22021 atatggcgtt taatagcccg aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg
    22081 gcgtggttcc gtggtcacca gaagccggta aagtgctggg tacgaaggcg cagattcagt
    22141 ttagtgccgg atcgctttcg ctcaatgatg ttcctgccac caatgtactg attgaaggca
    22201 gtattgataa cgatcgcgtt acgctgacta acctgggtgc cgacatcgcc cgcgggacat
    22261 taaccggaaa cgcgcagcgt aacgccgacg gcagctggca agtggaaaat ctgcgcatgg
    22321 cggatatccg tctacaaagc gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg
    22381 tcccgtcgtt gcagattggt cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact
    22441 gggcggtgac cgacctcgat ctcagcttgc gcaacatgac cttcagtaaa gatgactggc
    22501 agacacagga aggcaaactg tcgatgaacg ccagcgagtt catttatggt tcgctgcatt
    22561 tatttgaccc gattataaac gcggaatttt ccccgcaggg cgtagcgctg cgccagttca
    22621 ccagccgctg ggaagggggt atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa
    22681 cgttgatcct tgatgatgcg gcaattgccg ggctggaata taccttgccg aaaaactggc
    22741 aacagttgtg gatggaaacg acacccggtt ggttaaacag cctgcaactg aagagattta
    22801 gcgccagccg caatctgatc attgatatcg accctgactt cccgtggcag ctcaccgcgc
    22861 tcgatggtta cggtgccaac ctgacgctgg ttaccgatca taaatggggc gtctggagtg
    22921 gctcggcgaa tctgaatgcc gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt
    22981 cgctggcgct gaccgccaac agcagcacgg tgaatatcag cgaactgagt gcatttactg
    23041 aaaaaggcat tctggaagcc accgcaagtg tttcacaaac gccacaacgt cagacccata
    23101 tcagcctgaa tggacgcggt gtgccggtga atattttgca acaatgggga tggcctgaat
    23161 tacagttgac tggcgacggc aatattcagc ttaccgccag tggagatatt caggccaatg
    23221 ccccgctgaa acctacggtt agcgggcaat tgcatgccgt gaacgccgca aagcagcaag
    23281 tgactcaaac catgaatgcg ggcgtcgttt ccagcggtga agttacatcg acggagccgg
    23341 tgcggtaaac atcaatctcg ccgggtacga ctcagacgta cccggcattc cgtcaataga
    23401 ttacaacgta attgtcagcg cattcccttg aggcgtcacc accagcccct gctcactttc
    23461 agcctgcgaa ccgccttgca gaccatttac tttcacaaca ttgcgcaagc acagcgtcca
    23521 gttcttcgcc tcgcccgcac cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa
    23581 agtaaagatc accgatccgt cagcagcagg cacttcacag acggcttcat gcccgtcttg
    23641 cagattaaag aggtggaatg ccgtgccttc gtgccacacg taatcgggac gttgatcgtt
    23701 gttgcccagc gccagtagag tgttatcacg cacataaacg ggcagactca ggaagccgtg
    23761 ctgctgttta tgccagcgac taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg
    23821 accttccggc aggtagaact gcacatcgcc cgcttcagtg aacaccggcg caaccatcac
    23881 gttgtcgcct aacatgtatt gacggtcaag gtaatcacaa gccggatcgt ccgggaactc
    23941 catcatcatg gcccgcatca tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata
    24001 cagatacggc atcatgcggc atttcagttg cgtgaagaag cgcaccacat cacaggactc
    24061 atcatcgtag gcccacggca cacgataaga tttgctaccg tgtaaacggc tatggctgga
    24121 gagcaaacca aacgcgcacc agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc
    24181 gccgatatcg tggctccaga agccaaaacc tgaaaggcca atagacaaac caccgcgcag
    24241 gctttccgcc attgattcgt agttagcgta acaatcgcca ccccagtgta ccgggaattt
    24301 ctgcgcaccg acggaggccg agcgggcaaa caagacagct tcttcctcac caacggtgtc
    24361 cttgagcacg ttccacacca gttcgttgta gatgtacgca taatggttat gcattttctg
    24421 cggatcggaa ccgtcaaacc actgaacatc agttgggata cgttcgccaa agtcggtctt
    24481 aaagcaatca acgcccatcg cgaccagacc tttcagtttg tcggcgtacc atttgcaggc
    24541 atccggattg gtaaagtcat aaatcgccag acctggctgc catttatccc actgccatag
    24601 cgaaccgtcc gggcgtttga gtaaatagcc tttctcttgt aactctttaa agacggggga
    24661 tttttgaccg atataggggt taatccagac gcagattttc agccctttcg ctttcaggcg
    24721 gcggatcatc ccttccgggt cagggaaagt cagcgggtcc cactcaaaat cgcaccactg
    24781 gaaggctttc atccagaaac agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc
    24841 cataccatcg ataaagctgt ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt
    24901 tagccacagg ccgaaggacc acgggggcgg cagcgccgga cgaccagtaa agcgggtata
    24961 acgatcaagt accgctttcg gcgtcgggcc gtcgataaca aagtattcga gatattcact
    25021 ctcaacgctg aactgcactt tggagacttt ctccgatccc acttcaaaag agacacactg
    25081 gggatgattg accagtaccc cataaccacg gttagtcatg tagaacggga tatttttata
    25141 cgcctgttca gtacttgtgc cgccgtcccg gttccaggtc tctaccgtct ggccattgcg
    25201 caccagggca gtaaagcgct ctcccagacc gtaaactgtt tcgccaacgc caagatcaag
    25261 ccgctcaaac atataattgc gttgattatt cgtgtcctgc acgtagccat tatttttcac
    25321 ctgactaccg gtaatacgtt cgccgttgcg cagaaaatcc agtgaccaga actcaccttt
    25381 gctgacacgc gcgcttaagt tgccactttt aaactcagca taacgttctg tgttttcgat
    25441 tgtgaccttc acgtcctgca aaatattgag cggataatga ggaccgttat tcagcgcccc
    25501 ctgaaaatac tcaatccgca caccgacaat accttcctgt ggggagaaaa agcgcagcgt
    25561 aaataaaggc gtatcaagct gccaggtacg ttcacgcaca tcacgggggg cagcatagac
    25621 caccatttca ttatcctgct gttcaacctc gaatacctga agcgggtgaa tcaaattgag
    25681 cccaggttga atcaaccagt ttccatcgct aattttcatt gccgttcctt agttctgcac
    25741 ttcttgagag gtgaattgtt gctggcaacg gcgtttaccc tgagccagct gttccataac
    25801 ggttttcaga ttgtgcgtcg tgagtgagta gtagcgttta gcgataatcg cgctcagcaa
    25861 ataacagatc gccggaacaa tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt
    25921 ctgcgctttt tctgccgcat catatccgcc ataagccagc atccagccga taagagcgcc
    25981 gccgaaggcc aaccccagtt tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag
    26041 gcgtttacca ttgcaccact cgccgtagtc gacggtatcg aacatcatta cccactggat
    26101 aggtgtcacc agttgatgca acacaccaat cacgaagatg aagacaaaca tagtgatgct
    26161 ggcctgcatg ggaacaaaga acatcgcgag gctaatcact gccagcaggg cgttcgtcca
    26221 ccagaagata gtgactttac atttccagtc ggtcagaggt tttgccagtg cggaaccaat
    26281 caggttaccc acgcaataag tggtgagaaa agcgacaaac acttccggcg tgcccaaaat
    26341 ccatgtgacg taatacatca tcgccccacc gcgtacgcac accgccagga tattgaaaat
    26401 ggttagtaaa ccgacaatcc gccactggtc gttttgccag atatcacgta aatcttcccg
    26461 catagacgtt gttgtaggtg gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc
    26521 cagcatcatt aatgccacca cggaaaggac cacgataccg ccctggaaac cgagtggttt
    26581 attatcaccg ccaattaaat taaccagtgg catcatcaga acagtagaaa gcatgcctcc
    26641 cgcggtcgcc agcacaaaac gccaggattg cagcgagata cgctgagtcg ggtcattggt
    26701 gattacacca cccaatgcgc agtaagggat attgacgacg gtataaagta aggtaagtta
    26761 gggtgtaagt aattgctgca tagatcattt tgccgttcat actgagatct ggcgtgctat
    26821 aggccagtac acagacgatc ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt
    26881 taccccagcg agagcgcgtt cgatcggcca acaaccccat gcaaggatcg gaaatcgcat
    26941 ccagtgcacg agcgaccaaa aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat
    27001 cggtataaaa gaacatcata tataacatta cgttatcgaa aataatgtgg ctggcggcgt
    27061 ctcccatgcc ataaccaatt ttctctttaa cggacaacac ttcactcttc atctgctttt
    27121 ccttgatgct ataggggtgc tgagaccgat aacgcatttt gtaaaccatc cctgtgagag
    27181 cgaatattcc gatatctggt tattaattta tgattcttgt tttatgtgat cgtggtagcg
    27241 ttaattccgc tcatatatca ttgtaaaata tgggttttat ataaattata atgctttcgt
    27301 gataatacgc tgcgtttatt aggcggaaaa aactgatctg ggggatgtag aaactcaagg
    27361 aagtagctat aatgcgcccc gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc
    27421 cggtgaggcg gctggacttc aaatccagtt ggggccgcca gcggtcccgg gcaggttcga
    27481 ctcctgtgat cttccgccag tttacctctt ctgaactctc ccaatgtcaa taaatctctt
    27541 ctaatccctt gtatatcagt atttatttgt atcctgaggt caacctactt caccccgtat
    27601 caagtttctt ttgggggtac tttagggggt gttatttcct attgaaatac aagcaccccc
    27661 ataataggaa aaatgtgata cataacaacg gcttttaaca atatctttgt tatacaaggg
    27721 gtgtattggg ggtatcaggt tagttactgg cagaaatccc cctcaggaac atgaattcat
    27781 caaaagccgg aggatgtcat ggcattaacc gatatcaaag tgaaaaccgc aaaacccaaa
    27841 gacaaaccct ataagcttgc tgatggcggc ggtatgtatc tactgattaa cactaatggt
    27901 tctaaatact ggcgcatgaa gtaccgcttt gctggtaaag aaaagatgct ttctattggc
    27961 gtataccccg atgtaacgct agccgatgct cgcgagaaac gcagtgaagc caggaaactc
    28021 ctcgctgctg gaggcgatcc aggggaggca aaaaaggaag aaaaaatcgc gcagcaaatg
    28081 agtctgaaaa atacgttcga ggctattgct cgcgaatggc accagttaaa agctgatcgg
    28141 tggtcattac gctatcgtga tgaaatcatt gatacctttg aaaaagacat ttttccctac
    28201 attggcaagc gccctattgc cgaaatcaaa ccaatggagt tactggaagc actgcgtaaa
    28261 atggaaaaac gtggagctct ggagaaaatg cgcaaggtcc gccagcgctg tggtgaggtg
    28321 ttccgctatg caattgttac tggccgggca gactataacc cagcccccga tcttgccagt
    28381 gctttagcta caccgaaaaa agtacatttt ccctttctta ctgctaatga acttccccac
    28441 ttcctcaacg atctggcggg ttataccgga agtataatca ctaagacagc tactcagatc
    28501 attatgttaa ccggcgtacg aacacaagaa ctgcgttttg cacgctggga agatatcgat
    28561 tttgagacaa aattgtggga gatcccggca gaagtaatga agatgaagcg acctcatatt
    28621 gtgccgctct ctgagcaggt cattatgcta tttaagcagc ttgaaccaat ctcaaaacat
    28681 catcccctag tctttatcgg tagaaatgat ccccgcaaac caattagtaa ggaaagcatt
    28741 aaccaagtaa ttgaattact tggatataag ggaagactca cagggcacgg tttcagacac
    28801 accatgagta caattctgca tgaacaaggc tttaattccg ccttgattga aatgcagtta
    28861 gctcatgtgg ataaaaattc catcaggggt acctataatc atgccctgta tctcgatggc
    28921 cgccgtgaaa tgatgcaatg gtacgctgat tacattgatt cactctccag ccgggaaagt
    28981 taagtcagaa atacttttcc ctgatgtttt cctcaattaa gtattttatc ctctggccta
    29041 caaggctgga ggatttttgt tattatatta tttttttgcg tcttcaaact tattacaaaa
    29101 acataattaa aaacaaattg acatttttaa attggcaatt acaaaataca gccttgcagt
    29161 tatttcgata cctttagcaa aggaaaagtt gattcagata taatttagca tttcaatcat
    29221 ctgacatcat tataaaaaat gagattcaca taaagataaa attattgttc tcaaggaaag
    29281 catgaagggg cttcgtaagc aatattcatt cggtgccaag acaggacttg atacacagaa
    29341 tctcaacttt tatgctctca gactatatca ccacatgttt tacaggctca gaaaatgaac
    29401 gatagattat gctttgaagt gcacgataac aagggttatt tcgtatttcc ggatacctgg
    29461 tttggcccat tgctggggga gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc
    29521 agcgagacga gttacatcaa caaactccga cgccttgctc aacgggagcc ggactttatt
    29581 gatatacacg ctcatctggc atatgcattt ctggaacaga atgccccgcg taaggcactg
    29641 aacgccgctc ttaaaggact ggcggcaggt aatcgtatca tcccggaaag tttctgtggt
    29701 gaaataatct ggatgcatcc cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt
    29761 ctcgcaaatg tgcacttaca gcgccatcag gatgcggtta tgctcactga taaaatactg
    29821 gcatacaatc ctgaagataa tcagggggct cgctggttgt tgggttcaga gctgttacgc
    29881 accggcgatc atgagcgagc cttcagtgta ctgaaagagc atgctgatga attttcacct
    29941 tactggtatg aactcggtct cctgcatttt ctgaacgggg aacatgttaa ggccgccaca
    30001 gcttttcgcc atggctttgc cacaaacact tacatcgcag aaatgctgtg cggaaatctg
    30061 catccatttc cacttgctgt gtggcatgac ttctccggct ctctggatac ggcagaagat
    30121 tactatgcaa catactcacc attatgggga caatatccgg aagcattgct gtttgtaaac
    30181 tggctctata accattcctc cgtgctgcat gaacgatcag aaatcattaa atgcgccgaa
    30241 atgctgattc aggaagatga tttcgaaata tgcgaaagta ttttaagaca gcaggagcac
    30301 ctgtggaaaa gaattgacaa gacactttct gaggaaattg tacagaaatg cagaaatatg
    30361 aacggtgaat atatctggcc ctggatattg cccttttccg cagcagggat gaaacattca
    30421 agaatccagt atcagtagat attgcattag tgtaacttta aaacaagtaa taatttatta
    30481 tccaataata acactgtgtt ccttccccag gtgattaccc tggggttatt ttctgaaagt
    30541 ttcctttgca aaaggatagc gttgtaaatt actttaacga attactccat gaactcatta
    30601 aagttctttc gtaatacttt atcgtcaaac acgttatagc tggcatcaaa aaccacagat
    30661 ttaccctgcc agcgggtaat cccctcctct ggcgttttcg gttgaactgg tatccccgtc
    30721 caacacaggc agccattata aacagtaaaa aattgtattc gctgttatga aatcaatagt
    30781 cctgacgtac tgttgaatgt gcttgtgttc tgaacctcgt acaaccagta catttgatat
    30841 cgaattataa acaaaacatt gcatccaact tttttaatat ggacaatagt tgtccatacg
    30901 gtggttttat attcgataaa tcaatggatt tcattgagtc acaggactgg taatggatga
    30961 aaatgcttta aggtttgcct catactggcg caactcgctt gcagatgctg agtcaggaaa
    31021 gggctgtttt gaacggaaag acgcccaaaa tttcactcac tggcatggga tagcggcggg
    31081 acgtcttgac gaagcgattg tcaataaatt ttttaaggga gaaaaagacg atgtcgaaac
    31141 tgtcaatgtc atcttacgcc caaaagttta tttccggtta ctgcagcatg gtaaggaccg
    31201 ttccgcaggc acgcctgata ttgttacacc gatagtgacg ccagccttgc taagccgtga
    31261 gggtttttta tatccgacgc cagcgacctc cattcccaga gacctgcttg aacctttgcc
    31321 aaaaggagca ttttcgattg gtgagattgg gcagtatgac aaatacaaga cgacccatac
    31381 tgatcctacc cacgtaatgt ggacacggcc ctaagcgaga ttatggtttt caaattgttc
    31441 cgggctgaga ccgccacagg cactgtgacg acgccagcga ttgtaatcac actcgatata
    31501 attaaacacc gttgcccgca ttatttcccg gctgctaaag cgttccccgt ggatacattc
    31561 caccttcagc gaatgaaaga agctttccac acaggcattg tcataacagt agcctttcgc
    31621 actcatactg ccacgcaggt tgtgtcgctt cagcattgcc tgataatccc ctgaacagta
    31681 ttgaccacca cggtccgtat gaacaatgac gctttccggg cgtcttctcc gccacaacgc
    31741 catttgcagt gcatcacagg ccagttgtgc tgtcattcgc ggtgacatcg accagccaat
    31801 gacggcgcgt gaccacaggt cgatgactac tgcgagatac aaccagccct catcggtacg
    31861 caagtacgtg atgtcacccg cccacttctg gtttcgggcc actggcgctg aagtcctgct
    31921 ccagcagatt ctccaatacg ggcaggccat gtgcacggta gctgaccggg ctgaacttcc
    31981 ggctggcttt cgcccgcagc ccctgacgac gcaggctggc ggcaatggtt ttaatattga
    32041 actccggcag ttcgtcagca aggcggggag caccgtatcg ctgctttgcc tcaaagaatg
    32101 ccttatggac agcggcatcg caggtgagcc gaaactgttg gcgcaggctc atctggtgac
    32161 gacgcctgag ccagacatac cagccgctgc gggcaacccg aagtacacga cacatcgctt
    32221 tgatgctgaa ctctgcccga tgattttcga taaagacata cttcatttca ggcgcttcgc
    32281 gaagtatgtc gcggcctttt ggaggatgga cagttcctca gcctgctccg ccagttgtcg
    32341 tttaaggcgg acattttcag cggccagttc gctttcgcgc tctgacgaac tcatttgttg
    32401 ctgctgttta ctgcgccagg cataaagctg agattcatac aggctgagtt cacgggctgc
    32461 ggcggccaca ccgatgcgtt cagcgagttt cagggcttcg ttacgaaatt caggcgtatg
    32521 ttgtttacgg ggcttcttgc tgattgatac tggt
//
LOCUS       DAJTYI010000033        32216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYI010000033 DAJTYI010000000
VERSION     DAJTYI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..685
                     /locus_tag="OO564_001502"
     CDS             <1..685
                     /locus_tag="OO564_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6652671.1"
                     /translation="YYIYDTKFLTLYLEQTEMKNLPIGGVWKGKVKLHSNSPAQDYFA
                     NITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNG
                     NAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVR
                     NNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVI
                     FTPSLPTIN"
     gene            complement(773..2071)
                     /gene="exuT"
                     /locus_tag="OO564_001503"
     CDS             complement(773..2071)
                     /gene="exuT"
                     /locus_tag="OO564_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6652672.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2554..3966
                     /gene="uxaC"
                     /locus_tag="OO564_001504"
     CDS             2554..3966
                     /gene="uxaC"
                     /locus_tag="OO564_001504"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6652673.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3981..5468
                     /gene="uxaA"
                     /locus_tag="OO564_001505"
     CDS             3981..5468
                     /gene="uxaA"
                     /locus_tag="OO564_001505"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6652674.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5551..6102
                     /locus_tag="OO564_001506"
     CDS             5551..6102
                     /locus_tag="OO564_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6652675.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6107..7351)
                     /gene="sstT"
                     /locus_tag="OO564_001507"
     CDS             complement(6107..7351)
                     /gene="sstT"
                     /locus_tag="OO564_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6652676.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7947..8912)
                     /gene="alx"
                     /locus_tag="OO564_001508"
     CDS             complement(7947..8912)
                     /gene="alx"
                     /locus_tag="OO564_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6652677.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9183..9383)
                     /locus_tag="OO564_001509"
     CDS             complement(9183..9383)
                     /locus_tag="OO564_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6652678.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9414..10181)
                     /locus_tag="OO564_001510"
     CDS             complement(9414..10181)
                     /locus_tag="OO564_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6652679.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10260..10952)
                     /locus_tag="OO564_001511"
     CDS             complement(10260..10952)
                     /locus_tag="OO564_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6652680.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11029..11532)
                     /locus_tag="OO564_001512"
     CDS             complement(11029..11532)
                     /locus_tag="OO564_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6652681.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11617..12753
                     /gene="rlmG"
                     /locus_tag="OO564_001513"
     CDS             11617..12753
                     /gene="rlmG"
                     /locus_tag="OO564_001513"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6652682.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13038..13352
                     /gene="higB"
                     /locus_tag="OO564_001514"
     CDS             13038..13352
                     /gene="higB"
                     /locus_tag="OO564_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6652683.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13349..13765
                     /gene="higA"
                     /locus_tag="OO564_001515"
     CDS             13349..13765
                     /gene="higA"
                     /locus_tag="OO564_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6652684.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13810..15828)
                     /gene="fadH"
                     /locus_tag="OO564_001516"
     CDS             complement(13810..15828)
                     /gene="fadH"
                     /locus_tag="OO564_001516"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6652685.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16048..18399)
                     /gene="ygjK"
                     /locus_tag="OO564_001517"
                     /pseudo
     CDS             complement(16048..18399)
                     /gene="ygjK"
                     /locus_tag="OO564_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18416..18835)
                     /locus_tag="OO564_001518"
     CDS             complement(18416..18835)
                     /locus_tag="OO564_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652686.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18813..19166)
                     /locus_tag="OO564_001519"
     CDS             complement(18813..19166)
                     /locus_tag="OO564_001519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652687.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19167..19487)
                     /locus_tag="OO564_001520"
     CDS             complement(19167..19487)
                     /locus_tag="OO564_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652688.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19621..21054)
                     /locus_tag="OO564_001521"
     CDS             complement(19621..21054)
                     /locus_tag="OO564_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6652689.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21117..21566)
                     /locus_tag="OO564_001522"
     CDS             complement(21117..21566)
                     /locus_tag="OO564_001522"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6652690.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21563..24649)
                     /gene="ebgA"
                     /locus_tag="OO564_001523"
     CDS             complement(21563..24649)
                     /gene="ebgA"
                     /locus_tag="OO564_001523"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6652691.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24832..25815)
                     /gene="ebgR"
                     /locus_tag="OO564_001524"
     CDS             complement(24832..25815)
                     /gene="ebgR"
                     /locus_tag="OO564_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6652692.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26034..26365
                     /locus_tag="OO564_001525"
                     /pseudo
     CDS             26034..26365
                     /locus_tag="OO564_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26407..27786)
                     /gene="ygjG"
                     /locus_tag="OO564_001526"
     CDS             complement(26407..27786)
                     /gene="ygjG"
                     /locus_tag="OO564_001526"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6652693.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28203..29723
                     /gene="aer"
                     /locus_tag="OO564_001527"
     CDS             28203..29723
                     /gene="aer"
                     /locus_tag="OO564_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6652694.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29877..30500)
                     /gene="nfeR"
                     /locus_tag="OO564_001528"
                     /gene_synonym="yqjI"
     CDS             complement(29877..30500)
                     /gene="nfeR"
                     /locus_tag="OO564_001528"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6652695.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30777..31523
                     /gene="nfeF"
                     /locus_tag="OO564_001529"
                     /pseudo
     CDS             30777..31523
                     /gene="nfeF"
                     /locus_tag="OO564_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31577..31652)
                     /locus_tag="OO564_001530"
     tRNA            complement(31577..31652)
                     /locus_tag="OO564_001530"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31617..31619),aa:Ile,seq:cat)
     gene            31777..>32216
                     /gene="mug"
                     /locus_tag="OO564_001531"
     CDS             31777..>32216
                     /gene="mug"
                     /locus_tag="OO564_001531"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6652696.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRV"
BASE COUNT         7790 a         8409 c         8471 g         7546 t
ORIGIN      
        1 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
       61 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
      121 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
      181 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
      241 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
      301 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
      361 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
      421 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
      481 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
      541 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
      601 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
      661 gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa
      721 acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt
      781 gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg ttctgcaata
      841 cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg aacagcgggc
      901 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
      961 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     1021 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     1081 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     1141 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     1201 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     1261 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     1321 ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc agtgcgatac
     1381 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     1441 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     1501 ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     1561 cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc accagcggcg
     1621 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg atggaacgct
     1681 ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     1741 cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc agcgcggttg
     1801 caccacagaa cacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     1861 catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa taggctgcga
     1921 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     1981 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca
     2041 tataccaacg taacccttta attttactca tgtaaaacct catcgttttg ttatatccgc
     2101 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     2161 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     2221 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     2281 tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag agccatcaca
     2341 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     2401 tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     2461 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     2521 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     2581 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     2641 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     2701 ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg taccaacggt
     2761 gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     2821 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt cgaactgcgt
     2881 cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     2941 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     3001 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     3061 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     3121 ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     3181 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     3241 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg
     3301 aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag
     3361 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     3421 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     3481 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag
     3541 ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac
     3601 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     3661 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     3721 atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt tgtcggtatg
     3781 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     3841 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     3901 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     3961 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     4021 tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     4081 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     4141 acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     4201 acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     4261 ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg
     4321 ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca
     4381 acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta
     4441 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     4501 tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     4561 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     4621 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     4681 ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggaa
     4741 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     4801 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     4861 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     4921 acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg
     4981 cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc ggtatcacca
     5041 cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg
     5101 tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta
     5161 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     5221 ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc accaacagtg
     5281 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     5341 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta
     5401 agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     5461 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     5521 ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc ccagggcgtg
     5581 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
     5641 aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
     5701 tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
     5761 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
     5821 ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg
     5881 ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg
     5941 attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag ttttctgctt
     6001 atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt
     6061 aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa ttacgcaggg
     6121 cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag
     6181 ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata aagccgacgg
     6241 caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc agtgggatca
     6301 gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa gccactacgc
     6361 tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc gcagccagcg
     6421 tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc agcggaatag
     6481 aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata ttcaccggaa
     6541 tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc aggcacagca
     6601 gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca
     6661 gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg
     6721 tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg atcggtgcga
     6781 agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg accaggtttt
     6841 tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac accagaatcc
     6901 cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc attaccagtc
     6961 cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta ctggacaagt
     7021 gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca gagaaggtgc
     7081 ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc tggtggttag
     7141 caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc agtgcgccga
     7201 cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa
     7261 gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc
     7321 ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac
     7381 atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga
     7441 gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcatg
     7501 ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact ggctattaag
     7561 gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg cgctccttta
     7621 acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg ccaatcgccg
     7681 ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc
     7741 tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc
     7801 ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc ggtctttttg
     7861 taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa tgagttatct
     7921 ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg ccgataattc
     7981 acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg
     8041 gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa caccagaatc
     8101 accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac
     8161 atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg
     8221 gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct caattccacc
     8281 agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa
     8341 tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc
     8401 ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc
     8461 aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca gctgcctgtg
     8521 aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcgacgt
     8581 tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa gacgttatcg
     8641 accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg tggatcggca
     8701 acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa cagtaacgac
     8761 agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca
     8821 cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcaaatccg
     8881 ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc
     8941 caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc
     9001 tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga
     9061 tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag
     9121 gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc ttttacactg
     9181 ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg
     9241 cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg atgatccacc
     9301 agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt attaatatgc
     9361 tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac tttctagcat
     9421 tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct tcccaggaca
     9481 gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc catcaccacc
     9541 acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac gcttttcggc
     9601 tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg
     9661 ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc
     9721 gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc
     9781 tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa cagcaccacc
     9841 tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagcggt
     9901 ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg
     9961 gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc
    10021 gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg
    10081 cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg
    10141 cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata
    10201 aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat
    10261 tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga
    10321 tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg agcgagtttc
    10381 tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt aaacccgata
    10441 gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag
    10501 ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga atccagcgtt
    10561 gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc gatcaaagcc
    10621 tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact caccagtagc
    10681 catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag
    10741 cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac ggcgttgaca
    10801 tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat aaaaatggtc
    10861 gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga aaaacaaata
    10921 cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga aaggggggag
    10981 gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc
    11041 ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct ggtggtactg
    11101 cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct ccttcaggtg
    11161 cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt
    11221 agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag aaacagcggt
    11281 atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat
    11341 cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc agagagcttt
    11401 atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg
    11461 ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct gaagataagt
    11521 aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca
    11581 ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc
    11641 cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg
    11701 gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc cggcccggtg
    11761 ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac
    11821 agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg cctaaacggg
    11881 attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca acagccgggt
    11941 gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact gcgtgcgctg
    12001 cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac
    12061 acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg
    12121 aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag
    12181 accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc gaatgtcttc
    12241 tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc agagaatctc
    12301 gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat
    12361 aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc
    12421 cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt tatgatcaac
    12481 aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa ccctccgttt
    12541 caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca cgcccgccgc
    12601 tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga ttacttccat
    12661 aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa atttgtggtg
    12721 ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc cggatgcggc
    12781 gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata agacgcggca
    12841 agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat
    12901 ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca
    12961 cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc
    13021 aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa
    13081 aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt
    13141 tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg
    13201 ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt
    13261 ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta
    13321 ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg
    13381 agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacac
    13441 ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga
    13501 tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa
    13561 tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta tggatcaata
    13621 tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt
    13681 gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat
    13741 ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttca
    13801 aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc
    13861 cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag
    13921 cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc
    13981 taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata
    14041 acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc
    14101 ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg
    14161 ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct
    14221 gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg
    14281 ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt
    14341 gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct tcggatgatc
    14401 gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg tttcatcgaa
    14461 cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc
    14521 gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt
    14581 ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta cctgatgccc
    14641 acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga ccaccgccag
    14701 attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg cgcgaggatt
    14761 caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct ggttgcagcc
    14821 aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca gttccgcatc
    14881 cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc
    14941 ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga cgtggccttt
    15001 cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat
    15061 ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg cttcaatggc
    15121 ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt cgagcatcga
    15181 caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc
    15241 tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt
    15301 gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt cgacgccatc
    15361 gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat
    15421 ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggacgg
    15481 tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggcgatttt
    15541 gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc
    15601 gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa tgccgccgct
    15661 gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca gccgttcagc
    15721 accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc ggttttttaa
    15781 cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag
    15841 tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga aaagggaaaa
    15901 ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta acgccggatg
    15961 cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg ataaggcgtt
    16021 cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt tatacaacat
    16081 atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc ctgtcagcgg
    16141 gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc tgaagaacgt
    16201 atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt tcagaccaaa
    16261 ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa aagccggatt
    16321 ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat cgagcatcac
    16381 cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg gcgaccagcc
    16441 ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg ccagtggttt
    16501 atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac aggtgttgat
    16561 gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg gcttaccgag
    16621 gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg cctgatccac
    16681 cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt tttcggcgaa
    16741 tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca actgttcttt
    16801 gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg cagcgacctg
    16861 tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt agttgttcag
    16921 cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct cgccgctctc
    16981 ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc cgttatgatc
    17041 gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct ctgccaacca
    17101 ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa ggctgggttt
    17161 ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa ggttccacgc
    17221 tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag gctggatctg
    17281 ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat gcgccatcgc
    17341 aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc agcgtccggt
    17401 caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc agttaccgtt
    17461 gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat ccggattggt
    17521 taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat aaaacgccgg
    17581 acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag cggtcagcag
    17641 atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac tggtaaagcg
    17701 attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt ccgattcgcc
    17761 ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca ggccatcacg
    17821 ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt tatcggaaag
    17881 cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca ccagatccag
    17941 cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg cgaagcgcag
    18001 agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc cgggaatact
    18061 gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca ggcggtcgaa
    18121 attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg gaaagccgcc
    18181 catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat cgaaaaacgg
    18241 attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg caccagtacg
    18301 gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac ttatcagcag
    18361 agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt ccttattacc
    18421 agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca tttttcatgt
    18481 agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac cagataccaa
    18541 actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg gcgtaggtgt
    18601 aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc atcatcccgc
    18661 tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag gcgcgagcgg
    18721 caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg gtaatttcca
    18781 gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg ttcatccatt
    18841 gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag ccaggctttg
    18901 ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt gccgcttaag
    18961 ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta gcccaaatcg
    19021 ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg cagactgagc
    19081 atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat cccaccttcg
    19141 aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc ctgcacggtc
    19201 cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag gccaccttca
    19261 atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat atatccacca
    19321 agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat tgcgggtgtt
    19381 tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa ggcgtagcaa
    19441 aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt gttaatccat
    19501 gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa aggcagaaac
    19561 tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt attagtctgc
    19621 ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg ttaatccctt
    19681 tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac cgccgacgtt
    19741 ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga caaaccctac
    19801 ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg agcccatacg
    19861 gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca gcatgatgaa
    19921 taacggcgga agcatggacg ctgcggcggt catattaata atggtattca tcagatcctg
    19981 cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca gaaactggat
    20041 ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct taccaaagat
    20101 gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca tcagcaggga
    20161 accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg cttccggcag
    20221 accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa atttcaactc
    20281 tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta cgccgataaa
    20341 aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga cgtcgttaac
    20401 gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg taacgccgag
    20461 gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg caggctgtac
    20521 gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca gcagcatcag
    20581 cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg aaacccaggt
    20641 ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca taatatattc
    20701 gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg aggtgaaaaa
    20761 gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc cgcccagcga
    20821 actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa caaattctgc
    20881 gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca tcggtgccga
    20941 ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa cggcggcaaa
    21001 agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag acataacaca
    21061 tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg tagttgttat
    21121 ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt gaccgcgtta
    21181 ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc ttcgtggacc
    21241 tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg ataatattcc
    21301 actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg caggtagtaa
    21361 tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga gtcattttcc
    21421 acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat gttgtcgata
    21481 ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat ctggcggaac
    21541 tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc gtggaaaaga
    21601 accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg ccagaaaccg
    21661 gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag tccagcacct
    21721 cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc agggtgatgt
    21781 catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc tgggtatagt
    21841 gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg tcgtggcggt
    21901 tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg aagggatagt
    21961 tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc tgctggctgt
    22021 cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat tcgccgttaa
    22081 tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa cgctcgccgg
    22141 aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta cagcgcatcc
    22201 cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact tcaccatcgc
    22261 tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga ttcggctgcc
    22321 acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag ttgatctttg
    22381 gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg ccactcattt
    22441 ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga tcgtcttcca
    22501 gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg ttttccttca
    22561 gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg cgggaatctt
    22621 tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc gtgatctgca
    22681 agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc gcgagcgttt
    22741 caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta aaccacagtt
    22801 tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc gcgataacct
    22861 gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa ccatcaagac
    22921 agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac cagacattgc
    22981 cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa tgaccctgaa
    23041 tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt ccgttcccca
    23101 tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca ccaaactcat
    23161 tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca gcatcgcgat
    23221 cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg tacatcgcgc
    23281 ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc gacggatggt
    23341 ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc cactgcggat
    23401 cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg acgtcggttt
    23461 ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga tcgttcgggt
    23521 agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga tctttctcga
    23581 cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg ttgacgccgt
    23641 gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg atatcacgga
    23701 agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc agcgtcatga
    23761 ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg acagtaaaag
    23821 caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg ctgtgcacca
    23881 cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag gcggcgagat
    23941 tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca aagtcggtac
    24001 gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca tcgcggaaga
    24061 tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc cactgcatca
    24121 cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca aactctgcgg
    24181 tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact tcaaaatagg
    24241 tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg ctgagggtga
    24301 aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca tcgatgggga
    24361 acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc cacatggcgg
    24421 ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct ggtacttgca
    24481 gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac aggctgctgg
    24541 tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag tacgcacgcg
    24601 gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata ggacctcccg
    24661 gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct gggggggtta
    24721 gtaaaatttt tactaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata
    24781 aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga tttaacgggt
    24841 cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg cgcgaccatc
    24901 gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg
    24961 cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag
    25021 cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta cgccgatagc
    25081 aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt
    25141 tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac
    25201 cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg
    25261 ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat agaagtcgat
    25321 gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc
    25381 gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc gcagggcggg
    25441 cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta atccgttgtg
    25501 ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa tgccgtggcg
    25561 gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt agatagccag
    25621 aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg tcttgtactc
    25681 cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg
    25741 atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga ttgcgatgtc
    25801 ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc attgtagagg
    25861 aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga cgctgtaacg
    25921 gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag cctacaggat
    25981 tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa gcaatggaaa
    26041 ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga
    26101 aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg aaaaaacact
    26161 gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg ggaaaacggt
    26221 ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct
    26281 gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg agcggatgat
    26341 gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc gccatccgat
    26401 ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac gcgccgcttt
    26461 gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt
    26521 ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt
    26581 atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac cacgcgcttc
    26641 ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca acatatcgcc
    26701 tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg tcgccagcgc
    26761 cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg ggttgtcgaa
    26821 cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca cgccgccgcc
    26881 gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc aggcaaacat
    26941 cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg cgccgaactc
    27001 atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta cgccaccttc
    27061 gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt tgcactcatt
    27121 aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc ctggcagcag
    27181 tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc caagtgattt
    27241 accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg actgataagc
    27301 cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga agaagctgta
    27361 tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc gtagcggatc
    27421 gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg cggaaaccac
    27481 aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt cgataaactc
    27541 ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta cggctccgta
    27601 atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga catgctcttt
    27661 gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca gcgttcgctt
    27721 ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg aaggtaacct
    27781 gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt attgcaggga
    27841 ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg ttatcatttg
    27901 tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt tgtcgggtat
    27961 ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct gatttttggg
    28021 caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc aaagaattag
    28081 atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa gataaccgca
    28141 gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca caggacgcga
    28201 acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac gataccactc
    28261 tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact tttgtgcagg
    28321 tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg cgtcacccgg
    28381 atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg gagccctgga
    28441 gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg gccaatgcgg
    28501 taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc cgggcgacgg
    28561 atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga cgtaccagta
    28621 agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg ccttcattac
    28681 cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg gcggccatgc
    28741 tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg gtattgttgg
    28801 caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc cgtcaggcac
    28861 tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc gatgagctgg
    28921 ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta attaacgatg
    28981 tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa ggcaccgatg
    29041 aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg gcgaccatga
    29101 accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc gataaacttt
    29161 ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg gtgatcaaga
    29221 caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg ctgattaacg
    29281 atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg gcgcgtgccg
    29341 gcgaacaggg caaaggtttt gcagtggtgg caggggaagt gcgtcattta gccagccgca
    29401 gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat aaggtgcaat
    29461 ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg gcacaggtga
    29521 aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag gccgatgggc
    29581 tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa aatgccgagc
    29641 tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc cgactggaag
    29701 acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag cacaatgcct
    29761 gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt aggccggata
    29821 aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg cggcgattaa
    29881 tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt ttttatttgt
    29941 gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa attatccagc
    30001 gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc tttgatgcgt
    30061 tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc ttgttcggtc
    30121 agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga ctgctcctgc
    30181 agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc ctgggttaga
    30241 ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag aatatccaga
    30301 atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc gccgcgaccg
    30361 tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca gtgctcgtgt
    30421 tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg tttacaacac
    30481 ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat ctaatttata
    30541 tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag cacctcacat
    30601 ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac tgaatgcctt
    30661 cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat taacaatcat
    30721 tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg ataacaatga
    30781 ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt gaactgactg
    30841 tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc ggcgaggcgt
    30901 tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt cctcaacctg
    30961 acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg gaaggaccac
    31021 gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa ctggcgatcg
    31081 atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta cggtggcagg
    31141 tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg tctgcgatga
    31201 atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc
    31261 gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc tcgcgcacct
    31321 tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg cgcgtctggc
    31381 gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa ggtaaagtcg
    31441 ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg gtacgtgcag
    31501 cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac gatttctcgt
    31561 cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa gcgattatga
    31621 gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa gtaactccgt
    31681 gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat tttcaatccg
    31741 ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat tttggctcca
    31801 gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg gactggtttt
    31861 ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg gtttaccgac
    31921 cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg cgtcaccaaa
    31981 ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct gcacgcaggt
    32041 gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc gattctgggc
    32101 aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa acaaacgctc
    32161 accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag tcgcgt
//
LOCUS       DAJTYI010000034        32008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYI010000034 DAJTYI010000000
VERSION     DAJTYI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..514)
                     /locus_tag="OO564_001532"
     CDS             complement(<1..514)
                     /locus_tag="OO564_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6652697.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPG"
     gene            complement(692..1603)
                     /gene="gsiD"
                     /locus_tag="OO564_001533"
     CDS             complement(692..1603)
                     /gene="gsiD"
                     /locus_tag="OO564_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6652698.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1606..2526)
                     /gene="gsiC"
                     /locus_tag="OO564_001534"
     CDS             complement(1606..2526)
                     /gene="gsiC"
                     /locus_tag="OO564_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6652699.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2544..4082)
                     /gene="gsiB"
                     /locus_tag="OO564_001535"
     CDS             complement(2544..4082)
                     /gene="gsiB"
                     /locus_tag="OO564_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6652700.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4102..5973)
                     /gene="gsiA"
                     /locus_tag="OO564_001536"
     CDS             complement(4102..5973)
                     /gene="gsiA"
                     /locus_tag="OO564_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6652701.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5960..6925)
                     /gene="iaaA"
                     /locus_tag="OO564_001537"
     CDS             complement(5960..6925)
                     /gene="iaaA"
                     /locus_tag="OO564_001537"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6652702.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7129..8364
                     /gene="moeA"
                     /locus_tag="OO564_001538"
     CDS             7129..8364
                     /gene="moeA"
                     /locus_tag="OO564_001538"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6652703.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8364..9113
                     /gene="moeB"
                     /locus_tag="OO564_001539"
     CDS             8364..9113
                     /gene="moeB"
                     /locus_tag="OO564_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6652704.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9292..9954)
                     /gene="fsa"
                     /locus_tag="OO564_001540"
     CDS             complement(9292..9954)
                     /gene="fsa"
                     /locus_tag="OO564_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6652705.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10085..10984
                     /locus_tag="OO564_001541"
     CDS             10085..10984
                     /locus_tag="OO564_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6652706.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10990..13422
                     /locus_tag="OO564_001542"
     CDS             10990..13422
                     /locus_tag="OO564_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6652707.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVLPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13568..14383
                     /gene="ybiV"
                     /locus_tag="OO564_001543"
     CDS             13568..14383
                     /gene="ybiV"
                     /locus_tag="OO564_001543"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6652708.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14535..15800
                     /locus_tag="OO564_001544"
     CDS             14535..15800
                     /locus_tag="OO564_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6652709.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16041..17633)
                     /locus_tag="OO564_001545"
     CDS             complement(16041..17633)
                     /locus_tag="OO564_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6652710.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            17852..18772
                     /gene="ldtB"
                     /locus_tag="OO564_001546"
     CDS             17852..18772
                     /gene="ldtB"
                     /locus_tag="OO564_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6652711.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18830..19948)
                     /locus_tag="OO564_001547"
     CDS             complement(18830..19948)
                     /locus_tag="OO564_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6652712.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19945..20412)
                     /gene="mntR"
                     /locus_tag="OO564_001548"
     CDS             complement(19945..20412)
                     /gene="mntR"
                     /locus_tag="OO564_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6652713.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20598..20726
                     /gene="mntS"
                     /locus_tag="OO564_001549"
     CDS             20598..20726
                     /gene="mntS"
                     /locus_tag="OO564_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6652714.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20998..22581
                     /gene="opgE"
                     /locus_tag="OO564_001550"
     CDS             20998..22581
                     /gene="opgE"
                     /locus_tag="OO564_001550"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6652715.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22629..23144)
                     /gene="ompX"
                     /locus_tag="OO564_001551"
     CDS             complement(22629..23144)
                     /gene="ompX"
                     /locus_tag="OO564_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6652716.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23197..23262)
                     /locus_tag="OO564_001552"
     CDS             complement(23197..23262)
                     /locus_tag="OO564_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652717.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23497..24384
                     /gene="rhtA"
                     /locus_tag="OO564_001553"
     CDS             23497..24384
                     /gene="rhtA"
                     /locus_tag="OO564_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6652718.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24683..25186
                     /gene="dps"
                     /locus_tag="OO564_001554"
                     /gene_synonym="pexB"
     CDS             24683..25186
                     /gene="dps"
                     /locus_tag="OO564_001554"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6652719.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25590..26336
                     /gene="glnH"
                     /locus_tag="OO564_001555"
     CDS             25590..26336
                     /gene="glnH"
                     /locus_tag="OO564_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6652720.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26475..27134
                     /gene="glnP"
                     /locus_tag="OO564_001556"
     CDS             26475..27134
                     /gene="glnP"
                     /locus_tag="OO564_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6652721.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27131..27853
                     /gene="glnQ"
                     /locus_tag="OO564_001557"
     CDS             27131..27853
                     /gene="glnQ"
                     /locus_tag="OO564_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6652722.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28114..30338
                     /gene="ybiO"
                     /locus_tag="OO564_001558"
                     /pseudo
     CDS             28114..30338
                     /gene="ybiO"
                     /locus_tag="OO564_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30335..31261)
                     /gene="rlmF"
                     /locus_tag="OO564_001559"
     CDS             complement(30335..31261)
                     /gene="rlmF"
                     /locus_tag="OO564_001559"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6652723.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31537..31797
                     /locus_tag="OO564_001560"
     CDS             31537..31797
                     /locus_tag="OO564_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6652724.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         7810 a         8575 c         7961 g         7662 t
ORIGIN      
        1 tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag
       61 ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt
      121 cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc
      181 gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca
      241 tcatttccat tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca
      301 gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt
      361 tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat
      421 agttaatcat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca
      481 gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat
      541 ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg
      601 attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt
      661 gattaccctt tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat
      721 cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg
      781 gaaaaacagc gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc
      841 cccactctgg tgtcggcggc tgcgcaccta ggccgagaaa tgacaggctg gcggcagaga
      901 taatcgaggt gccaatgcgc atggtgaaaa acaccacgat agaagagacg gtcccaggca
      961 ggatatgacg caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa
     1021 aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac gcggggatgg
     1081 aaaaaatggc gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag
     1141 cgatcgccag taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc
     1201 gatcccacca gccttcataa tatccagcca acaagcccag caacgtccct atcgccacac
     1261 cgataaacac ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc
     1321 tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat
     1381 tcagattgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa
     1441 tggccaccac aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc
     1501 gaaatcgtcg ccagaattca tgccacggtg tacgtacctg gtcaggtttg accagtggca
     1561 tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat
     1621 agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag
     1681 cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta cagagtcaac
     1741 gagtaagcgt ccaagtccgg gccagttgaa aactttctcc accacgatag aacctccgag
     1801 caaaaagcca aactgtaagc ccatcatggt cactaccggg atcatcgcgt tgcgtagccc
     1861 gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc
     1921 ttcgcttaaa acgtcgacaa atgacgcacg ggtaaagcgc gccatcacgg cggccactgc
     1981 cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacagtagg
     2041 cagccagccc agttcaacgg agaatacctg aattaaaaac atccccagcg caaatgccgg
     2101 aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca
     2161 gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat
     2221 ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga
     2281 caccatcgac aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg
     2341 atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc
     2401 ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atgtggacaa ataaaaacac
     2461 cagcaccgag acgataaaca gcgtcggaat caaccccagt aatcgtttga taacgtaatt
     2521 aagcattcca ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccgctg
     2581 tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc
     2641 accagcggga tccacggcga ttcttgccag atgatatcct gcgccgcctt atataagcgg
     2701 gtcttttccg ccggatcatt cgttttcagt gcctgagcca ggaagtcatc cacctgttta
     2761 ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt tttgcgaggc aaacagcggc
     2821 gatagtgccc agtcggcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg
     2881 ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg
     2941 gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc
     3001 tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc
     3061 gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc
     3121 gcgtaggcga tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca
     3181 actttcacca gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta
     3241 tcgaacggct tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc
     3301 atcaactcga tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa
     3361 gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga
     3421 cgccaggtta tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc
     3481 accttcacaa aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga
     3541 tgaaaaccaa tctccttgcc atatttttcc agcgctgtcg gtgaaatcat cgcggtcgcc
     3601 ggatgggcaa gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt
     3661 gtcggatcga tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga
     3721 ttcgccggat cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg
     3781 ccatcctgga atttaattcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa
     3841 acggtataac tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc
     3901 ccctggtaaa acgatttcgc tacggcctga gataacgtgt cattcgcgtc atacggatcg
     3961 agcgtggtga aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat
     4021 gccatcaacg ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc
     4081 attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc
     4141 gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag
     4201 atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg
     4261 ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa
     4321 gaccgcgcac cgtgaaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg
     4381 actaatccgc tctaccacag ccatatcgtg ggagataaac agatacgcaa tgccgaaatc
     4441 acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga
     4501 aacggcttcg tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat
     4561 gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag
     4621 gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc
     4681 gtgtacacgc agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc
     4741 gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccaggtga
     4801 caaggtatcg attcgctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg
     4861 cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt
     4921 ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg
     4981 attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga ttacgcaccc gtaaaacagg
     5041 ttcgccatcc accaccgttt tctgctcgat ggggggttcc tgtttcgctg gatgttcaag
     5101 cgatatcaat gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac
     5161 agcagctaac agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt
     5221 acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac
     5281 cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt
     5341 gattaattgc aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc
     5401 aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc
     5461 gccagagagt tgatgcgaat aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg
     5521 atccagcatc cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg
     5581 aattgattcg gcaatctgtt cacccacagt aaatactggg ttcagcgatg tcatcggctc
     5641 ctgaaatatc atcgccatat ccgcaccgcg cacatggcgc atttgtgcag cgctctgctc
     5701 gctaagttca atcacatcac gactgcgccg ccgcaacagc attttatcgc actgtactaa
     5761 accgcccgcc tgttccaaca gacgcatcaa tgccagcgca gtcactgact taccggagcc
     5821 ggattcgcct acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac
     5881 cgcagctatt ttctgctggt cctgcataaa ggcaatatta agattttcaa ccgccagcac
     5941 attaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac
     6001 cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg
     6061 gtagcgcgac attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag
     6121 ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca
     6181 ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgcct gtgccggtac
     6241 aagaaaccgc cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc
     6301 caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccagattgc
     6361 cgtctaaatc caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg
     6421 caccgctatg gtcgaggact gttgcccctt cctcgcgcgc tgccagtagt tgttcataac
     6481 gcaaaggcgt ggagaaaatc tccggtgaga cgcgctccat gccatgagca aacgcaaaat
     6541 tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg
     6601 caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac
     6661 catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc
     6721 cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg
     6781 cactttcgcc ggcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcga
     6841 tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat
     6901 gaattgcaat gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg
     6961 ctataaatat cagaattttt atgaattact cattcaattt ttgagtatag aaagatggat
     7021 atggcatgta aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tatggcgttt
     7081 ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc
     7141 accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt taccccactg
     7201 accacccagg aaacgctgcc actggttcag tgttttggtc gtattctggc gagcgatgtc
     7261 gtttcgccac tggatgtccc ggggtttgat aactccgcta tggacggcta cgcggtgcgt
     7321 ttagccgata ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag
     7381 ccataccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg
     7441 ccggaaggct gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg
     7501 cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct
     7561 gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt
     7621 gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct
     7681 accggtgatg aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc
     7741 aaccgtctcg ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg
     7801 attatccgcg acgatccaca tgccctgcgc gccgcattta ttgaagccga cagccaggcg
     7861 gatgtagtga tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt
     7921 cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc
     7981 gcattcggta aactcagcaa tagctggttc tgcggcctgc cgggcaaccc gatttcagcg
     8041 acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc
     8101 agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga
     8161 cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg
     8221 accaccggac atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc
     8281 gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt
     8341 aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca
     8401 accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc
     8461 gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg
     8521 ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac
     8581 gccagacact gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg
     8641 ccctgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg
     8701 cagaacttgc agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg
     8761 cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg
     8821 cggcaattcg tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct
     8881 atcgctgcct tagccgtttg tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga
     8941 tggcaccgtt gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg
     9001 caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt
     9061 ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac
     9121 ctccaggcct gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg
     9181 cggcgtgaac gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat
     9241 cggtgcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga
     9301 cgttctgcca aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc
     9361 cggatagcta atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatccggc
     9421 cagtaagcag tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg
     9481 cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc
     9541 ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag
     9601 cagcccttgt gccgcgccat ataccgcggt tcccagcgtc ggaatccctt ccgcttttaa
     9661 catcttaatc gctgccagcc cctcggctgt caccggaact ttcaccacga tatccgcaat
     9721 aatagaacgc agcttaagcg cgtcattcac catcccttca gcagtggtag ccattacctg
     9781 ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag
     9841 cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat
     9901 acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat
     9961 cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg
    10021 acgcacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc
    10081 aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt
    10141 cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc
    10201 ccgtacgcag gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc
    10261 taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa
    10321 gttaaccccg gagcatctga cggcgttaac cgactgctgt ccgacacagg cgttaaccgt
    10381 gtgtggtgaa gtgaaaagcg ttgaggagat catggcgacc gttctgcgcg ataaaccgtt
    10441 ttacgatcgc agcggcggcg gcttaacgct ttcgggtggt gaacccttca tgcagccgga
    10501 aatggcgacg gcgctgctgc aagcctgcca cgaggcaggc attcatactg cggtagaaac
    10561 ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct
    10621 tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc
    10681 cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt
    10741 gccgctgatt cagggcttta atgccgacga aacctctgta aaagccatta ccgattttgc
    10801 cgccgacgag ctgcacgttg gcgaaattca ttttctgccc taccacacgc tgggcatcaa
    10861 caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga
    10921 gctgctcgat tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg
    10981 ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa
    11041 aatgcgctgg tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc
    11101 gagatgtatc aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg
    11161 catcacctgg cgaatcgcac catctggatc aaacactatg agttgatcat tggcaaccag
    11221 gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa
    11281 gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa
    11341 cgtgttctgc atgaagtgtg cccgtggtgg cgcggtcaga cggtacagga tcgctgctat
    11401 ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gcatcattaa agcggaaggc
    11461 aatatgacct ccggcgatgc gcacctggcg gtcaatttcc cgctgctgct ggaaaaaggg
    11521 cttgatggtc tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtgctg
    11581 gaagatttgc acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagt
    11641 gaacacattg aacgtttcgc tgccctggcg cgtgcaatgg ccgcgaccga aacccgcgaa
    11701 agccgtcgcg atgaactgct ggcgatggca gaaaactgcg atcttatcgc ccaccagccg
    11761 ccgcagactt tctggcaggc gctgcaactg tgttacttca tccagttgat tttgcagatt
    11821 gaatctaacg gtcactcggt gtcgtttggt cgtatggatc agtatctcta cccgtactat
    11881 cgccgcgacg ttgaactgaa ccagacgctg gatcgcgaac acgccatcga attgctgcat
    11941 agctgctggt tgaaattgct ggaagtgaac aaaatccgct ccggctccca ctcaaaagcc
    12001 tctgcgggaa gtccgctgta tcagaacgtc accattggcg gacaaaatct ggttgatggt
    12061 caaccaatgg acgcggtgaa tccactctct tacgcgatcc tcgaatcctg cggtcgccta
    12121 cgttccactc agcctaacct cagcgtgcgt taccatgcag gaatgagtaa cgatttcctc
    12181 gacgcctgcg tacaggtgat ccgctgcggc ttcgggatgc cggcattcaa caacgacgaa
    12241 atcgtgctcc cggaatttat taaactcggt attgaaccgc aggacgctta tgactacgca
    12301 gcgattggtt gtatagaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg
    12361 agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc
    12421 agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggtaactt caacaacttc
    12481 gatgaagtga tggccgcgtg ggatacgcaa atccgttact acacgcgtaa atcaatcgaa
    12541 atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg
    12601 ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac
    12661 tgggtttctg gcctgcaggt cggtattgcc aacctcggca acagcctggc ggcagtgaag
    12721 aaactggtgt ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat
    12781 gacttcgatg gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag
    12841 tacggcaacg acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc
    12901 gacgaactga aacagtacca taacccacgc tacggtcgtg gtccggttgg cggcaactat
    12961 tacgcgggta cgtcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaacg
    13021 ccggacgggc gtaaagccca caccccgctg gcagaaggcg ctagtccggc ctctggtact
    13081 gaccatctcg gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt
    13141 ctcggcggcg tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac
    13201 aagcagaaac tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt
    13261 cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac
    13321 cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct ctctccagac
    13381 gctcaggacg atatcatcgc ccgtactgaa catatgctgt aatctcttcg gcccggctgc
    13441 ggcgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat
    13501 aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag
    13561 cacacctatg agcgtaaaag ttatcgtcac agatatggac ggtacttttc ttaacgacgc
    13621 caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat
    13681 taagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct
    13741 aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca
    13801 actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa
    13861 agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc
    13921 ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaac ctgtaaaaga
    13981 ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccgg atgaacaaat
    14041 cccgttagtg atcgacaaac tacacatagc gctggatggc attatgaaac ccgtcaccag
    14101 tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg
    14161 gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa
    14221 cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa
    14281 cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt
    14341 gattcaggct gtgctggata acacatcccc ttttaacagc tgacctctct accggagcca
    14401 tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc
    14461 attaacttat ttataacttg tattgtcaca tgcaaatcag catagtttcg aatgaaagtt
    14521 aatgcgaggt ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag
    14581 cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgatgtc cagcagatct
    14641 ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac
    14701 agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg
    14761 cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc
    14821 gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctgaaccgc aaccgctttg
    14881 acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg
    14941 agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg
    15001 cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt
    15061 ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccttccg cccggaacga
    15121 cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag
    15181 cgtatcagca cgttttcgcc aacgtcttta atggcaatct ggcgaaatac gatccctggc
    15241 atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg
    15301 tgtttcggac attccagggc tggactgcgc tctcggatat gctgcctggt caggggttgc
    15361 tgcacgtcgt gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg
    15421 atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc
    15481 aatggcatcc actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact
    15541 ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt
    15601 ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc
    15661 acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact
    15721 atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag
    15781 cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt aagaaaatcg
    15841 attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg cggcgttaac
    15901 gccttatccg gcctacaaag ttctgcagat tcaatatatt acaggtactg cgtaggcctg
    15961 ataagcgtag cgcatcaggc tattttgcat ttgtcatcag ccgtcgtcac cccgacagta
    16021 tcggggtgac ggataatcac ttactcgatc cctttactac gcaggtaatc ttcgtaatta
    16081 ccgctaaagt cgatcacgca ttccggggtg atttccagaa tgcgggtcgc cagggagctt
    16141 acgaactcac ggtcgtgtga aacaaagatc agcgtgccct gatacagttc cagcgccatg
    16201 ttcagtgact cgatggattc catatccagg tggttggtcg gttcgtccat gatcagaatg
    16261 ttcggcttct gcatcattaa cttaccaaac agcatccgcc ctttttcccc accggaaagc
    16321 actttagctg gctttttgat gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt
    16381 accgcctgct cgtcatcgcc ttcctgcttc cactgactca tccattcgaa cacggtcaga
    16441 tcattttcaa actcatattc gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac
    16501 cattttacgg tgccgctgtc cggttgcaga tcgcccacca gcgttttcag cagcgttgat
    16561 ttaccgacgc cgttggtacc cagtaccgca agtttttcac cgacctccag cagcaggttg
    16621 agatttttaa acagtggacc gttatcaaac cctttggtca gaccttccac ttccagcgcg
    16681 ttacggaaca gtttcttatc ctgttcaaaa cggatgaacg ggttctgacg gctggaggct
    16741 ttcacctctt ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat
    16801 ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca actcagcaat ctgcgctttc
    16861 ttcttggcgt tatcggccag cagacgttca cgcgcctggg tcgccgccgt catgtactca
    16921 tcgtagttac ccggataaac gcgcagctcg ccgtaatcca gatccgccat gtgggtacag
    16981 accatgttaa ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc
    17041 agcacctgtt ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc
    17101 aggagaatat ccggctcagc aaacagcgcc tgcgccagaa gcacacgcag cttccagcca
    17161 ggagcaactt cactcatcgg gccgtagtgc tgttccactg gaattcccac gccaagcaac
    17221 agttcaccgg cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt cacttccaga
    17281 tcggccactt tatagccgtc ttcttcactc atttccggca aagcatagat gcggtcgcgc
    17341 tcctgcttca cttcccacaa ctctttatgc cccatgatca ccgtatccag cacagtgaac
    17401 ccttcaaagg caaactgatc ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa
    17461 acgttaccca gcgtcggctc aaggtcgccg ccgagaatct tcataaaggt ggatttacca
    17521 ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt
    17581 tcaaacaacg gcttactgcc gaactgcatg gtgacgttac tggaaactaa cacaggcgta
    17641 tcctgaaaag agatatgaca aaccgcgcat tatgccataa gtctgaaaga agatcctgct
    17701 cctcgcttaa ggtctacact caagccaaag gcaaaaaatg tgatttcgta cacatctgat
    17761 ttcactgtga gctggaatga acttataatg cgcttccaat actctaatat tctcaaccca
    17821 atggcctgcc aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa cattattcgc
    17881 agcggccttc gctgttgtcg gcttttgcag taccgcctct gcggtaactt atcctctgcc
    17941 aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc accattcctg aaggtaacac
    18001 tcagccgctg gagtattttg ccgcggagta ccagatgggg ctttccaata tgatggaagc
    18061 gaacccgggt gtggatacct tcctgccgaa aggcggtact gtactgaaca ttccgcagca
    18121 gctgatcctg ccggataccg ttcatgaagg catcgtcatt aacagtgcag agatgcgtct
    18181 gtattactat ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt
    18241 aggcaaagat acgcctatca actggaccac caaagttgag cataagaaag caggcccgac
    18301 ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac cgctaccggc
    18361 tgtcgttccg gcaggtccgg ataacccgat gggtctgtat gcactctaca tcggtcgcct
    18421 gtatgctatc catggcacca acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg
    18481 tgtgcgtcta cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg tcggtacccg
    18541 cgtacagttt attgatgagc cggtaaaagc gaccaccgag ccagacggca gccgttatat
    18601 tgaagtccat aacccgctgt ctaccaccga agcccagttt gaaggtcagg aaatcgtgcc
    18661 aattaccctg acgaagagcg tgcagacagt gaccggtcag ccagatgttg accaggttgt
    18721 tcttgatgaa gcgatcaaaa atcgttccgg gatgccggtt cgcctgaatt aatcgtcaaa
    18781 acgtaaaaca aaggcggact cataatccgc cttttttatt tgccagacct taccaggccg
    18841 ggatcataac taacaaaacg tatcccacca gcgccgccca caacagcatc ggtattgaat
    18901 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    18961 ccagcgatcc tggtaataaa ccaaagcccc ccacgtttac cgcccatgcc agtaataaag
    19021 acggaggtac ataattgagc agcaaaatgg tactcggcac gttactgatc acctgcgata
    19081 aaccgactgc cgttaaccat aacccgggtt cagataaatg actcacgtta cccaacacgc
    19141 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    19201 gcagcaacgt ccagtccaca ctcaacacca cgcgacgcgc cagcagcaca aagcccgccg
    19261 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aaaacgatat
    19321 acagccccag acaattccac accagccgcg gtttccactc cggtgtttgc atccccgtat
    19381 ggtattgcag cgcctttcca gggaaacaac accagcacaa cagcaggagt gtcagcatca
    19441 ttgcgccagc cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    19501 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    19561 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    19621 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    19681 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    19741 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    19801 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    19861 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    19921 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    19981 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    20041 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    20101 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    20161 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    20221 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    20281 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    20341 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc
    20401 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    20461 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctatat
    20521 aatatagcct gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    20581 gcaagtcagg aggtcttatg aatgagttca agaggtgtat gcgcgtgttt agtcattctc
    20641 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    20701 agcaagataa accttcagat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    20761 atgcaggcat aacgcgtttt ggtctgaaaa aaaccacttt ttgtcggatt tgcaatcccc
    20821 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    20881 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    20941 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttaccata gtggcatatg
    21001 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    21061 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    21121 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    21181 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    21241 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    21301 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    21361 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    21421 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    21481 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    21541 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    21601 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggta
    21661 gtgatcggtg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    21721 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc
    21781 tccacgcaga aatcgcttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    21841 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    21901 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    21961 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    22021 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    22081 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    22141 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    22201 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    22261 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    22321 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcggcga taaagcgcat
    22381 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    22441 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    22501 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    22561 attttcgacc cgaaacctta aaaacaaaaa tccgccccga gaggcggatt tttatatcac
    22621 caaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    22681 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    22741 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    22801 gtctggaatt taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg
    22861 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    22921 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    22981 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    23041 ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta cggaagtgcc tgcggtgaaa
    23101 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    23161 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    23221 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    23281 atttaaatca ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat
    23341 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    23401 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    23461 cgctttactt aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    23521 ccggtctggt taccaatagt catattactc gttgccatgg cgtctattca gggtggagcc
    23581 tcgttagcta agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg
    23641 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    23701 gagcaacggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    23761 tatctttcta ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca
    23821 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    23881 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    23941 ggctgtgcgc tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    24001 cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta
    24061 attttcgtgc caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt
    24121 ccattgggtc tggctgtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt
    24181 gccctcaccc gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg
    24241 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    24301 ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa
    24361 ataaaagaat tagacattaa ttaaatttac atttctgtat ggttatgcat aaccatgcag
    24421 aatttctcgc tacttttcct ctacaccgtc tttatatatc gaattatgca aaagcatatt
    24481 tattccgaaa attcctggcg agcagataaa taagaattgt tcttatcaat atatctaact
    24541 cattgaatct ttattagttt tgtttttcac gcttgttacc actattagtg tgataggaac
    24601 agccagaata gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat
    24661 aacatcaaga ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa
    24721 tctgctttat acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct
    24781 gaatcgccag gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa
    24841 catgcgcggc gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact
    24901 gatcgatcat ctggatacca tggcagaacg tgccgtgcag ctgggcggtg tagctctggg
    24961 gaccactcaa gttatcaaca gcaaaacccc gctgaaaagt tacccgctgg acatccacaa
    25021 cgttcaggat cacctgaaag aactggctga ccgttacgca atcgtcgcta atgacgtacg
    25081 caaagcgatt ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg
    25141 cgacctggat aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgctgatg
    25201 gtgcagaact ttagtacccg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca
    25261 gcccacctca acgcacttat ttagtgcatc catctgctat ccccagctga ttaagtaaat
    25321 tttttgtatc cacatcatca cacaaccgtt acataaagat tgttttttca tcaggtttta
    25381 cgctaaataa tcactgtgtt gagtgcacaa ttttagcgca ccagattggt gccccagaat
    25441 ggtgcatctt cagggtattg ctctataaat cgtgcatcac gtttttgccg catctcgaaa
    25501 aatcaatgag ttgcaaaact ggcacgattt tttcatatat gtgaatgtca cgcaggggat
    25561 cgtcccgtgg atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg
    25621 cactgaccct ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga
    25681 cggataccgc cttcgttccg tttgaattta aacagggcga taaatatgtg ggctttgacg
    25741 ttgatctgtg ggctgccatc gctaaagagc tgaagctgga ttacgaactg aagccgatgg
    25801 atttcagtgg gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca
    25861 ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg
    25921 gcctgttagt gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg
    25981 ggaaagtggt tgctgtgaag agcggtactg gctccgttga ttacgcgaaa gcaaacatca
    26041 aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa ctgggcacca
    26101 accgcgcaga cgccgttctg cacgatacgc caaacattct gtacttcatc aaaaccgccg
    26161 gtaacggtca gttcaaagcg gtaggtgact ctctggaagc gcagcaatac ggtattgcgt
    26221 tcccgaaagg tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg
    26281 agaacggaac ttacaacgaa gtctacaaaa aatggttcag tactgaaccg aaataataac
    26341 gctacacctg taaagcgcac tggcagttcc ctctcccccc tggggagagg attagggtga
    26401 ggggcgcaaa cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt
    26461 aacaggaaca acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga
    26521 ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg tctggctggc ggtctggtaa
    26581 tcggattgct ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc
    26641 tggtctttat tgaagtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt
    26701 tcgccctgcc gatggcgttt aacgacttac gcatcgaccc atttactgcg gcggtggtca
    26761 ccatcatgat caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta
    26821 tccacaaagg ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc
    26881 gctacgtcat tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga
    26941 tcatcagcat taaagacacc tcactgttta ttgttatcgg cgtggcggaa ctgacccgtc
    27001 aggggcaaga aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg
    27061 tgttctatct gattattacc ctggtgctga gctttattct gcgtcgtctg gaaagaagga
    27121 tgaaaatcct gtgattgaat ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct
    27181 gcacaatatc gatttgaaca ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg
    27241 ttccggtaaa tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cctccggcga
    27301 tctgattgtc gatggcctga aggttaacga tccgaaagtt gacgagcgcc tgattcgcca
    27361 ggaagcaggt atggtgttcc agcagtttta cctcttcccg catctgacgg cgctgggaaa
    27421 cgtcatgttt ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc
    27481 gcgtgagctg ctggcgaaag tcggtctggc agaacgtgca catcactacc cttccgaact
    27541 ttctggtggt caacagcagc gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat
    27601 gatgctgttt gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa
    27661 ggttatgcag gatctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg
    27721 ttttgccgag aaagtagctt cgcggctgat ctttatcgac aaaggccgga ttgcggaaga
    27781 tggcaatccg caggtgttga tcaagaaccc gccgagccag cgcttgcagg aatttttgca
    27841 gcacgtctct taataagaca cattgcctga tgcgctacgc ttatcaggcc tacaggatat
    27901 ctggcaactt attaaaattg catgaacttg taggacggat aaggcgttca cgccgcatcc
    27961 ggcaaaaaag cccgcacgtt gtcagcaacc tgcttaatat cccttcctcc ctttcacccg
    28021 aaagggaggc acaccagatt cctctcattt aaaatcgccc ctcccccagc atctatactt
    28081 atctttttgc tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc
    28141 tgcctgctgg gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca
    28201 acggactcaa cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg
    28261 gcgcactggc ggatgtgctg gataacgaca cctcgcgtaa agagttgatc gaccagttgc
    28321 gtactgtcgc cgcgacgccc cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg
    28381 ttgaagagca aaccgtgctg caaaaggtca ccgaagtcag ccgccattat ggtgaagccc
    28441 tttccgcccg cttcgggcaa ctttatcgca atatcaccgg ctccccgcat aagccattta
    28501 atccacaaac cttcagcaat gcgctgagcc atttttcaat gttagcggta ttagtgtttg
    28561 gtttttactg gctgattcgc ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg
    28621 cgaggcaaaa aaatcgtgag cgcagcaact ggttgcagct tccggcgatg attattgggg
    28681 cgtttattat cgacctgctg ttactggccc tgacattatt tgtcggccag gtattaagcg
    28741 acaacctgaa tgcaggtagt cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct
    28801 ttgccctgat tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg
    28861 agctgcgccc gttcacgatt caggacgaga gcgcccgtta ctggagtcgc cgcctgagct
    28921 ggttaagcag cctgattggc tatggcctga ttgtggccgt gccgattatc tctaatcagg
    28981 tgaatgtaca gataggtgcg ctggcgaacg tcatcattat gctgtgcatg accgtctggg
    29041 cgttgtacct gatcttccga aataaaaaag agattaccca gcatttgctc aactttgcgg
    29101 agcattcgct ggcttttttc agcctgttta tccgcgcctt tgcgctggtg tggcactggc
    29161 tggcaagcgc ctattttatc gtgctgtttt tcttttcgtt gttcgatccg ggcaacagtc
    29221 tgaaatttat gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcgtttg
    29281 tttccggtat gttttcccgc tggctggcga aaaccatcac cctctcgcca catactcagc
    29341 gtaactatcc ggagctacaa aaacggctga atggctggct ttcggcggcg ctgaaaacgg
    29401 cgcgtattct gaccgtctgc gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg
    29461 atttctggaa ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca
    29521 tcgcactcat tcttttcttc tcggcggttg gctggacggt gctcgccagt ttgatcgaaa
    29581 accggctggc ttcggatatt catggccgcc cgctacccag cgcccgtacg cgtaccctgc
    29641 tgacgctgtt ccgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt
    29701 tgtcggaaat cggcgtcaat atcgcgccat tgctggcagg tgccggggca ttaggtctgg
    29761 ctatctcgtt tggttcgcaa acgctggtaa aagatattat caccggggta tttattcagt
    29821 ttgaaaacgg catgaacact ggagatttgg tgactatcgg gccgttgacc ggcacagtgg
    29881 aacggatgtc gattcgctcc gtgggcgtgc gccaggatac cggggcgtat cacatcattc
    29941 cgtggtcttc gataaccacc tttgctaact tcgtccgcgg tattggttcg gtagtggcaa
    30001 actacgatgt tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg
    30061 taacggaatt gatggaaaac gaagaaattc gcgggctgat tattggtgaa ccgaactttg
    30121 ccgggattgt cggcttaagc aataccgcgt ttacactgcg tgtttcgttc accacgctgc
    30181 cactcaaaca gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc
    30241 tggcgggcgt tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc
    30301 cggctgaacc gctgccgccg ggggagccaa cgctttaacg ttggcgatta acaaaacggc
    30361 ggcgctgctc gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg
    30421 ccatctcttt ttttaccacc ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg
    30481 gtaagttttc accacgagat accagcgagg taaaccacat cacctgcttc gcgaagcctt
    30541 tgctctcttc aatcatcttt ttgataaagt tgacttcacc gccttcacac cacaactcct
    30601 gttgctgacc accaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac
    30661 gctcactacc tgcccgcgcc gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg
    30721 cgtcgtattg ctcgtttttg tggatgatgc cgttaaaaat cgccccactc tctttttgcc
    30781 gacgcagacg aatggcgcgg ttaagccccg gattagcact gataatcgcc tgcgcactgc
    30841 ttaacgcctg gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa
    30901 tcagcggata aatacagttc gcaccaacgc cgatatccag aatgctggca ttcgctggaa
    30961 ttgttccgct tgcttcagcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg
    31021 gtactggtgg gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg taaaaatggg
    31081 ccagcaacgc cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct
    31141 cccctgcggg tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg
    31201 cgagatcgta gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca
    31261 tagcattctc ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct
    31321 ggctgcgttt atcattcccc aaaaccttaa catggctatg gtttatggca attcatcata
    31381 aaaatcagca ttatgcatgt cattgatttg tattattttg taaaaatata tcgttcaaaa
    31441 aataagcata tctctgtcaa ttttttgcgt catttgcatc gcgcaggcga cctacactta
    31501 tcaggcacta cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg
    31561 attgccaccg tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac
    31621 ctggacagcg gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta
    31681 agcgatctgg aggataaact ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc
    31741 attaattccg caggcggaaa tgaccagatg ttcggtactg caaccatcta caaataaccc
    31801 caccaacgta actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac
    31861 atcaaatatg aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa
    31921 atcgcgtcaa gtgataatgc ttatcaaaat tattatcact tgcacaaaca ctatcacggg
    31981 attaacagtg gcatcgcatc cgcagaga
//
LOCUS       DAJTYI010000035        31794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYI010000035 DAJTYI010000000
VERSION     DAJTYI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..370)
                     /gene="ssb1"
                     /locus_tag="OO564_001561"
     CDS             complement(<1..370)
                     /gene="ssb1"
                     /locus_tag="OO564_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6652725.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPA"
     gene            624..3446
                     /gene="uvrA"
                     /locus_tag="OO564_001562"
     CDS             624..3446
                     /gene="uvrA"
                     /locus_tag="OO564_001562"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6652726.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3481..3837)
                     /locus_tag="OO564_001563"
     CDS             complement(3481..3837)
                     /locus_tag="OO564_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6652727.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3841..4257)
                     /locus_tag="OO564_001564"
     CDS             complement(3841..4257)
                     /locus_tag="OO564_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6652728.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4368..5081)
                     /gene="aphA"
                     /locus_tag="OO564_001565"
     CDS             complement(4368..5081)
                     /gene="aphA"
                     /locus_tag="OO564_001565"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6652729.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5483..5705
                     /locus_tag="OO564_001566"
                     /pseudo
     CDS             5483..5705
                     /locus_tag="OO564_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6209..7402)
                     /gene="tyrB"
                     /locus_tag="OO564_001567"
     CDS             complement(6209..7402)
                     /gene="tyrB"
                     /locus_tag="OO564_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6652730.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7655..8734)
                     /gene="alr"
                     /locus_tag="OO564_001568"
     CDS             complement(7655..8734)
                     /gene="alr"
                     /locus_tag="OO564_001568"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6652731.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8787..10202)
                     /gene="dnaB"
                     /locus_tag="OO564_001569"
     CDS             complement(8787..10202)
                     /gene="dnaB"
                     /locus_tag="OO564_001569"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6652732.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGSQYDDE"
     gene            10285..11268
                     /locus_tag="OO564_001570"
     CDS             10285..11268
                     /locus_tag="OO564_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6652733.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11434..11676)
                     /gene="pspG"
                     /locus_tag="OO564_001571"
     CDS             complement(11434..11676)
                     /gene="pspG"
                     /locus_tag="OO564_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6652734.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11810..12847)
                     /gene="dusA"
                     /locus_tag="OO564_001572"
     CDS             complement(11810..12847)
                     /gene="dusA"
                     /locus_tag="OO564_001572"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6652735.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13210..14195)
                     /locus_tag="OO564_001573"
                     /pseudo
     CDS             complement(13210..14195)
                     /locus_tag="OO564_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14513..15028
                     /gene="zur"
                     /locus_tag="OO564_001574"
     CDS             14513..15028
                     /gene="zur"
                     /locus_tag="OO564_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6652736.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15070..15279)
                     /locus_tag="OO564_001575"
     CDS             complement(15070..15279)
                     /locus_tag="OO564_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6652737.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15395..16720)
                     /gene="dinF"
                     /locus_tag="OO564_001576"
     CDS             complement(15395..16720)
                     /gene="dinF"
                     /locus_tag="OO564_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6652738.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16793..17401)
                     /gene="lexA"
                     /locus_tag="OO564_001577"
     CDS             complement(16793..17401)
                     /gene="lexA"
                     /locus_tag="OO564_001577"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6652739.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17511..17879)
                     /gene="dgkA"
                     /locus_tag="OO564_001578"
     CDS             complement(17511..17879)
                     /gene="dgkA"
                     /locus_tag="OO564_001578"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6652740.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18050..20473
                     /gene="plsB"
                     /locus_tag="OO564_001579"
     CDS             18050..20473
                     /gene="plsB"
                     /locus_tag="OO564_001579"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6652741.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALPMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20628..21500)
                     /gene="ubiA"
                     /locus_tag="OO564_001580"
     CDS             complement(20628..21500)
                     /gene="ubiA"
                     /locus_tag="OO564_001580"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6652742.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21513..22010)
                     /gene="ubiC"
                     /locus_tag="OO564_001581"
     CDS             complement(21513..22010)
                     /gene="ubiC"
                     /locus_tag="OO564_001581"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6652743.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22233..23813)
                     /locus_tag="OO564_001582"
                     /pseudo
     CDS             complement(22233..23813)
                     /locus_tag="OO564_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24042..24962)
                     /gene="malM"
                     /locus_tag="OO564_001583"
     CDS             complement(24042..24962)
                     /gene="malM"
                     /locus_tag="OO564_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6652744.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25114..26454)
                     /gene="lamB"
                     /locus_tag="OO564_001584"
     CDS             complement(25114..26454)
                     /gene="lamB"
                     /locus_tag="OO564_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6652745.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26526..27641)
                     /gene="malK"
                     /locus_tag="OO564_001585"
     CDS             complement(26526..27641)
                     /gene="malK"
                     /locus_tag="OO564_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6652746.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28006..29196
                     /gene="malE"
                     /locus_tag="OO564_001586"
     CDS             28006..29196
                     /gene="malE"
                     /locus_tag="OO564_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6652747.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29350..30894
                     /gene="malF"
                     /locus_tag="OO564_001587"
     CDS             29350..30894
                     /gene="malF"
                     /locus_tag="OO564_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6652748.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30909..>31794
                     /gene="malG"
                     /locus_tag="OO564_001588"
     CDS             30909..>31794
                     /gene="malG"
                     /locus_tag="OO564_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6652749.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVK"
BASE COUNT         8102 a         8153 c         7699 g         7840 t
ORIGIN      
        1 ctgccggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg ccaacgttca
       61 ccacgacttc tgtggtgtag cgatcctgac cggattgatc ggtccattta cgggtacgca
      121 gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctcgcc acttctgcca
      181 gtttgccgaa cagcacaacg cggtgccatt cagtctgttc tttcatctcg ccggtcgctt
      241 tatcacgcca ggattcggaa gtagccagcg taatgttggc aactgcgcca ccatttggca
      301 tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg tttacgcctc
      361 tgctggccat gttcgtgtct cctgaaaaaa atcgttctga ataagtgtaa acgcgcgatt
      421 gtaccattac caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg
      481 ccagacattg ttacacaaca ctccggataa tgcattccaa tactgtatat tcattcaggt
      541 caatttgtgt cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa
      601 agcgtttaat ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata
      661 atctcaaaaa catcaacctc gttatccccc gcgacaagct cattgtcgtg accgggcttt
      721 cgggttctgg caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt
      781 acgttgaatc cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg
      841 tcgatcatat tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata
      901 acccgcgttc tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgttcg
      961 cccgcgtcgg cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg caaaccgtca
     1021 gccagatggt ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg ctgctcgcgc
     1081 caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg gcaagccagg
     1141 gttacatccg tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg
     1201 aactgcaaaa gaaacatacc attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc
     1261 ttacccaacg tcttgcggag tcgtttgaaa ccgcgctgga actttccggt ggtacagcgg
     1321 tagtggcgga tatggacgac ccgaaagcgg aagagctgct gttctccgcc aacttcgcct
     1381 gcccaatttg cggctacagt atgcgtgaac tggagccgcg actgttttcg tttaacaacc
     1441 cggcaggtgc ctgcccgacc tgtgacggcc ttggcgtaca gcaatatttc gatcctgacc
     1501 gcgtgatcca aaaccccgag ctgtcactgg ctggcggtgc gatccgtggc tgggatcgcc
     1561 gcaacttcta ttacttccag atgctgaaat cgctggcaga tcactataag ttcgacgtcg
     1621 aagcgccgtg gggcagcctg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca
     1681 aagaaaacat tgaattcaaa tacatgaacg atcgtggcga tacctccatc cgtcgtcatc
     1741 cgttcgaagg cgtgctgcac aatatggagc gccgttataa agagacagaa tccagtgcgg
     1801 tacgtgaaga attagccaag tttatcagca atcgcccatg cgccagctgc gaagggacgc
     1861 gtctgcgtcg ggaagcgcgc cacgtttatg tcgagaatac gccgctgcct gctatctccg
     1921 acatgagcat cggtcatgcg atggaattct tcaacaatct caaactcgct ggtcagcggg
     1981 cgaagattgc ggaaaaaatt cttaaagaga tcggcgatcg cctgaaattc ctcgtaaacg
     2041 tcggcctgaa ttacctgaca ctttcccgct cggcagaaac actttccggc ggtgaagccc
     2101 agcgtatccg tctggcgagc cagattggtg cgggcctggt tggcgttatg tacgtgctgg
     2161 acgagccgtc tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc
     2221 atctgcgcga tctcggtaat accgtgattg tggtggagca tgacgaagac gcaattcgcg
     2281 ccgctgacca tgtgatcgat atcggtcctg gtgcgggtgt tcacggcggt gaagtggtcg
     2341 cagaaggtcc gctggaagcg attatggcgg tgcctgaatc gttgaccggg cagtacatga
     2401 gcggtaaacg caagattgaa gtgccgaaga aacgcgttcc ggcgaatcca gaaaaagtgc
     2461 tgaagctgac aggcgcacgc ggcaacaacc tgaaggacgt gacgctcacg ctgccagtcg
     2521 gtctgtttac ctgcatcaca ggggtttcag gttccggtaa atcgacgctg attaacgaca
     2581 cactgttccc gattgcccaa cgccagttga atggtgcgac catcgccgaa ccggcaccgt
     2641 atcgcgatat tcagggactg gagcatttcg ataaagtgat cgatatcgac caaagcccaa
     2701 ttggtcgtac tccacgttct aacccggcga cctataccgg cgtgtttacg cctgtgcgcg
     2761 aactgtttgc gggcgtaccg gaatcccgtg cgcgtggtta tacgccagga cgtttcagct
     2821 ttaacgtccg tggcgggcgc tgcgaagcct gtcagggcga cggtgtgatc aaagtggaga
     2881 tgcacttcct gccggatatc tacgtgccgt gcgatcagtg caaaggtaaa cgctataacc
     2941 gtgaaacgct ggaaattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca
     3001 tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag ctgcaaacgt
     3061 tgatggacgt tggtctgacg tacattcgcc tggggcagtc cgcaaccaca ctttctggtg
     3121 gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc
     3181 tgtatattct cgacgagccg accaccggtc tgcacttcgc cgatattcag caactcctcg
     3241 acgtgctgca taaactgcgc gatcagggca ataccattgt ggtgattgag cacaatctcg
     3301 acgtgatcaa aaccgctgac tggattgtcg acctgggacc agaaggcggc agtggcggcg
     3361 gcgaaatcct cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcgcatacgg
     3421 cacgcttcct caagccgatg ctgtaatcgt taaggccgct ttctgagcgg ccttttcctt
     3481 tcagagttgt accagcaatt tacgcttttc ttccggtagt aaattcactg cctgctgata
     3541 agacgcatcc accagataat agatttgcga atccggcagc gaaccgtcga gatacacggt
     3601 gctccagtgc gctttattca gatggcggct aggacgcaca tcgctgtgct gctgacgtag
     3661 cagctccgcc agctccgggc tggttttcag cgaaacagct gggcgatttt ctacttcttt
     3721 caccatcgca aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac
     3781 gctctgttct gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt
     3841 ttactcccct tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc gatgttcccc
     3901 cagccagatg ccttgccaag tgccggtctg aatacgccct ttatgtaccg gcaatacaag
     3961 cgatgttccc agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc
     4021 atgctcataa tttccgttgt cgggaacggt gcggaggaaa aaacactcca tgtcgtggcg
     4081 tacggtggga tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag
     4141 atgcagtaag ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt
     4201 taccagatga aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg
     4261 ctgctccttg ataaaactct cttaatcagt ttgcagcaag acagcgaaag gataaaggtg
     4321 tgattaaaaa aacagcattc gggagagcat cactctctcc cgctctgtca gtattctgaa
     4381 ttgacgatca cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg
     4441 gaggcgcgca gaatgcggat accacgagcg ccgacatcgc gtgcggcggt aatatcgtta
     4501 tcggaatcgc cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt
     4561 tgccctggtt tatcgcccgc aaagattacc ggattcatgt tggtggcagg aatatgaaaa
     4621 ttatccgcca gcgtttttga aaccgtttct gttttcgtcg ggctacgacc agtcacaaag
     4681 aagatcgcgt caccgcggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga
     4741 atgctgaatt catcccagcc attattcatt ttttcccaga acacaggatt tttcagataa
     4801 tcttcgcttt ctggcgagaa ggtttttttg ccgcgccaga accccggact ggaaaaaagt
     4861 accgtgtcat cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca
     4921 atttgtgcga ccgaaaccca atgaattggt gcctgttcag caagcctggc aacgttagtc
     4981 ccagggttaa gcggtgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac
     5041 aataagcaaa cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca
     5101 gtatgttgaa actttttatt gtgagatttg ttgcaaaaac catctgacca taacgccagc
     5161 tgccggggaa aggaaggatt atctgcggtt tttgtgatga aaagcaggat gattatattt
     5221 aacccagtaa atacgttcct gtcttttttt gtataaaaaa tgtttattac tcgatgtaag
     5281 gatacattaa ttttacgttt attatgatga atcctcggca aatgagcgaa atttgataga
     5341 acatttatct gctgtacgat ttaatgaact taatgataca gcattacaaa aacaatcgaa
     5401 gtttataaag atgatttctg attgaccagc cccctaccct acaatggtga ttaattttat
     5461 tactcgcgat ggagatgatg atatgaatat atcatatgtg aatagcaaca aaacgacatc
     5521 tttacctgtt gagctagatg cactaaataa taaagatatc tcttatgcca aagatttctc
     5581 ttatgccaaa gatttctttt tatacataga aacacagtta aaaattgcaa aagatttttg
     5641 tagacctgga gaagaagtat caagttctat tgcaagtaaa gtttttcacg catttattga
     5701 tttagttaac aaaatcaggg gtaagaaaga ctttatgtat atttgcacgc tttgctgttt
     5761 tgccgaggag gttaaaggtg attattctca ttacaggacc tttttatttg atattggtaa
     5821 tcaatataag gttaaactta tacaaagcgg aaaaaaagag ttctctttaa cattagaatt
     5881 taacgatact ataattgaat ctcagaaagt cacaggcaat aaggcaaagc atattcttga
     5941 ggatatagaa atattctatc gtaacaaacc cgatacttat tattaaattt aataatggtt
     6001 agatgtgcag aaattattac tccacacgaa aaaataggga aaggaattcc agataattgg
     6061 gataaactta acaataaaag aactgacaac gttttattat ctctcacgta gaacaacggc
     6121 atcaaaaaaa tcatctacaa agaaatagtg aaagcgacga cagtttaatt tcactgcagg
     6181 ctgggtaact ccagcctgct ttcctgcatt acatcaccgc agcaaacgcc tttgccacac
     6241 gttgtacatt tgccgtattt aacccggcga cacacatgcg accgctggcg atgagataga
     6301 caccaaattc ttcacgtagt cggtcaacct gagcggcact taaaccggta taactgaaca
     6361 tgccgcgctg attaagcaga taatcgaaat tgcgttctgg catctctgtg cttaatacct
     6421 tcaccaattc ctgacgcatt gccagaatgc gagtacgcat ctcttctact tccgccagcc
     6481 agctggcttt caatgcctcg tcattcagca ctgcagccac cacctgcgca ccaaaattcg
     6541 gcgggctgga gtagttgcgg cgaactgttg ctttcaattg ccccagtacg cggccagcgg
     6601 cttcggcatc ttcacacaga acagaaagtc cgccgacgcg ctcgccgtaa agggagaaaa
     6661 ttttcgagaa cgaattgctc accagagcgg gtaatccagc gctggcaatg gcgcgaatag
     6721 cgtaggcatc ctcttccata ccggcactaa atccttgata ggcaatatcg aggaatggga
     6781 taagctcgcg ggctttgaga atttcaatca ccgcatccca ctgatcatta gtgagatcgg
     6841 cacccgttgg gttgtggcaa catggatgca gcaacacaat actgcgggca ggtaatgttt
     6901 tcagcgtcgc caacaggtca ttaaagcgca cgctgttagt cacttcgtca taccaggggt
     6961 aagtactcac ttcgaatcca gccccggcga atattgctac gtggttttcc caagtaggat
     7021 cgctgaccca gacgcctgat tccgggaagt agcgtttcag gaaatccgcg cccactttca
     7081 atgcccctga gccgccaagg gtttgaatgg ttgctacgcg ctgttgttgc agtaccggat
     7141 ggtccgcacc aaacagcagc ggcgcaatgg catggcgata gctgttaagc ccttccatcg
     7201 gtaaataaag cgaagcgcca tggggctgcg cattcaggcg cgcttccgcc tccgccacgg
     7261 ctttcaattg tggaataatt ccgtcttcgt tgtagtacag accgatactt aaattcactt
     7321 tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt aagaatcggg tcgccagcgt
     7381 aggcgtcaac tttttgaaac acgcgatggt tctccaggtt tacgggcagg tggttaaaac
     7441 acaataaacc ggaagaaggc gaagatcgag tggatgttca ggagcgaacg gcaattagca
     7501 acagagtgag actaatgata aacgtacatc cgccagaggc acgaccttta taagagcatg
     7561 aaagaacatc aacttattga atttttagga ttttattggc cggataaggc attcacgccg
     7621 catccggcac agacaatcaa atattacaga acgattaatc cacgtatttc atcgcgaccc
     7681 ttgaagtcag gcgcgtaata agttcgtaag cgcttacttt cgtcatttca gcgatacgtt
     7741 ctacgggcaa accttcgccc cataaaatga ccggatcccc ggctttgtcc tgcgcctgtg
     7801 gacctaagtc tacgcagatc atatccatcg ccacgcgccc gacaatcggt acttcgcgac
     7861 cgttcaccag cactggcgta ccggacggcg cggcgcgcgg ataaccatcg ccatagccca
     7921 tcgcgactac gccaagacgg gtatcacgtt cgcttaccca ggttccacca taaccaacag
     7981 gctctccggc tttatgctca cgcacggcaa tcaggctgga ggttagtgac atcactggct
     8041 gacagccaaa atcggcaccg gtggagcgat cttccagcgg cgagacgcca taaagaatga
     8101 tgcccgggcg cacccagtca aaatgcgact gtggccacag cagaatgcca cccgacgcgg
     8161 caatggaacg ttgaccaggt ttgccttcgc aaaaggtatt aaagatagcg agttgtttct
     8221 cggttgcgcc acattttggt tcatccgcgc gcgcaaaatg gctgacgata ttcaccggct
     8281 gacgaacgtt tttgcactgg gtcaggcgat gataaaacgc ctcagcctgt tccggcctta
     8341 cgcccagacg gtgcataccg gtatcgagtt tcatccagac ggtaaccggc tcgtccaagc
     8401 tagcctcttc cagcgcagcc agctgttctt cgttatgcac ggcggtatga aaatgttgcg
     8461 cagaaatcgt cggcagatct ctggcatcaa aaaagccttc gagtaacagt acaggtttgg
     8521 tgattccccc cgcacgcagt cgcagagctt cttcgagacg ggctacgcca aaggcgtcag
     8581 catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc ataagcgttc gctttcacca
     8641 ccgcaaccat tttactggca ggtgccagtt cacgaagacg ttgcaggttg tgtcgcagag
     8701 cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt tccttgataa ttgtttgctt
     8761 taattaccta attcataaaa taattattat tcgtcgtcgt actgcgaccc cgcatagttg
     8821 tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg tcccgattgg gccgttacgt
     8881 tgtttaccga taataatttc cgcgatgcct tttaaatcac tgttttcgtg atacacctca
     8941 tcacgataga taaacatgat caagtccgca tcctgctcga tagagccaga ttcacgcagg
     9001 tcggagttga ccggacgttt gtcggcacgt tgttccagag aacggttcaa ctgggacagc
     9061 gccaccaccg gcacgttcag ttctttcgcc agtgctttca gcgagcggga gatttctgca
     9121 atttccagcg tacggttatc ggaaagcgcc ggtacgcgca tcagttgcag gtagtcgatc
     9181 atgataagcc cgatgccgcc gtgttcacgg gcaatacggc gtgcgcggga acgcacttcc
     9241 gttggcgtca ggccggaaga gtcatcgata tagatattgc gtttttcgag caaaataccc
     9301 atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct gcccggtacg gattttagtc
     9361 tggtcaacgc gcgacagcga cgccagagaa cgcatcatga tctgttctga tggcatctcc
     9421 agcgagaaga taagtactgg tttatcctgc aacattgccg cgttttcgac gaggttcatc
     9481 gcaaatgttg ttttacccat cgacggacgc gcggcgacga tgatcaaatc cgacggctgc
     9541 aagccagcgg tttttttgtt gagatcgtca taaccggtgt ttaccccggt aacgccatcg
     9601 tgtggctgct gaaacaactg ctcaatacgc gccacggttg cgtcgagcac atcggcgatg
     9661 ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa ttttaaagac gcgggattca
     9721 gcaaggtcca gcagatcttc gctggtacgc ccctgcggat caaaaccggc ttcagcaatc
     9781 tcattcgcaa ccgagatcat ctcacggaca acggcacgtt cacgcacgat gtccgcataa
     9841 gcactgatgt tcgccgcact tggcgtattt tttgacagct ctgccagata agcaaaacca
     9901 ccgacgctat cgagttgccc ctggcgttcc aacgattccg caagggtaat cagatcgata
     9961 ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga tatgacggtg tgggcgggtg
    10021 taaaaatcgt ctgctaccac acgctcggct acatcatccc agcgttcgtt atccagcatt
    10081 aaaccgccca acaccgactg ctccgcttcg atcgagtgcg gaggcacttt cagcccggca
    10141 acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt tgaagggttt atttcctgcc
    10201 atagtgaatg gagttaccga aataaagaat gggtcgaaac tttaccatat gaagcagacc
    10261 ctgacgatac gttctggagg acacatggca acacgaattg aatttcacaa gcacggtggc
    10321 ccggaagtac ttcaagccgt agagttcact cctgccgatc cggcggagaa tgaaatccag
    10381 gtcgaaaata aagccatcgg catcaatttt atcgacacgt atatccgcag cggcctttac
    10441 ccgccgccat cgctacccag cggattaggt accgaagcgg caggcatcgt gagtaaagtc
    10501 ggcagtggtg taaagcatat taaggctggc gatcatgtag tctatgcgca gtcggcgtta
    10561 ggcgcttaca gctctgtgca taacattaat gcggataaag cggcgattct gcctgcggca
    10621 atttcttttg agcaacctgc ggcatccttc ctgaaaggct taacggttta ttatctgctg
    10681 cgcaaaacct atgaaattaa acccgatgaa cagttcctgt tccacgcagc ggctggcggc
    10741 gttggcttaa ttgcctgcca gtgggcaaaa gccctgggcg cgaaactcat cggcaccgta
    10801 ggaaccgcgc aaaaagcgca gagcgcgcta aaagccggcg cgtggcaggt tattaactat
    10861 cgtgaagaga atctggtcga gcgattgaaa gagatcaccg gcggtaagaa agtgcgcgta
    10921 gtgtacgatt ccgtgggcag agacacctgg gaacggtcgc tggattgcct gcaacgccgc
    10981 ggcttaatgg tcagttttgg caactcatca ggtgcggtta ccggtgtgaa cttaggcatt
    11041 ctcaatcaaa aaggctcgtt gtatgtgaca cgcccttccc tgcaaggcta tatcaccacg
    11101 cgggaggaat taaccgaggc cagtaatgaa ctgttctctt tgattgccag cggtgtgatt
    11161 aaggtcgatg tcgccgagca gcagaaatat ccgctgaagg atgcgcagcg tgcgcatgag
    11221 attctggaaa gccgggcgac gcaaggttcc agcctgctga ttccataaaa gaaatagggc
    11281 ttccacctgg gaagcccttt ctttttatag ttcggctgta tgtagggtac agcacgatga
    11341 atctgttaga ggcgcaatag tgacagattt gattatcaat tcctattttg ttctaaggat
    11401 aaaaccttag gttgtgatca tccgcacaat cccttagtaa cgccagcggt cataacgctg
    11461 atatttcggc atttttggtg ctttaatcgc cttaataacc cacaccaccg caatcgccag
    11521 tagtaaccac ggcagcaact taatcatcaa tgccagcata ccgccgagga acataatggc
    11581 cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa acgccggtga ccatcagcat
    11641 gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg ctctcccaaa tatgaaatct
    11701 cttgctggca ttacaagaat catgccaaaa ataatctatt gatttaacag caaaacgccc
    11761 cgcgacggtg cgcagggcgt ggtgaatttg actacttttt ggtgaaaagt taacgcttat
    11821 ccgccaccag tttgagcgcg tgttccagca cgttaatgtc agcacccgct ttatgggcat
    11881 tttcacttaa ataacgccgc cactgccgcg cgccaggaat accctggaac aaacccagca
    11941 tatgccggat aatatggccg agatacgtcc cctggctgag ttcatgctca atgtacggat
    12001 acatggcacg cactactgcc accggatcgg catcggtatc cgaggaacca aagatctccc
    12061 ggtctaccgc cgccagaata cccggattct gatacgcctc gcgcccgacc atcacgccat
    12121 ccatatgttg caaatgcgct ttagcttctt ccagcgactt gataccaccg ttaatcgaca
    12181 ttgtcagatg cgggaagtca cgcttcagtt gatacacacg cggataatcg agcggcggga
    12241 tttcacggtt ttctttcggg cttaaccccg aaagccaggc tttacgtgcg tggatgatga
    12301 acatctcaca ctcgcctttg ccggaaacgg tattgatgaa atcgcagaga aattcatagc
    12361 tgtcctggtc gtcgatgcca atacgcgttt tcaccgtcac cggaatcgac accacatcgc
    12421 gcatcgcttt cacgcagtcg gcaaccagct gcgcattacc catcagacac gcaccaaaca
    12481 tgccgttctg cacccggtca gacgggcagc cgacattcag gttgatctca tcatatccgc
    12541 gcgcttctgc cagctttgca cactgcgcca gcgccgccgg atcgctaccg cccagttgca
    12601 acgctaccgg atgttcttct tcactgtacg ccagataatc acctttaccg tgaataatcg
    12661 cccctgtggt caccatttcg gtataaagca acgtattgcg ggaaagcaga cgcaagaaat
    12721 agcggcaatg tctgtccgtc cagtcgagca ttggtgcaac actaaaccga ccactccagt
    12781 gaacgtcagt tttttcaggc attgcgctgg tttggttgat tttttgcatt tcagaattac
    12841 cgtgcatttt caaatgtaga gattatttta tcgatatatc atggggttat gttattcagc
    12901 ttcactgttc aggaggctca atagcggggt actataccat aacaacagga agcgcctgtc
    12961 tcattgcaaa agaaaattga gatcctctca aggcatgaag ctctcacgaa gtgatggaaa
    13021 taatcttatt agccgttaac tttgttaagg ccaatgataa acaatccagg ttcgacgata
    13081 aataaaaaat cacacattaa actctggtga tatatctccc tgctaatagc attgatagag
    13141 aaaaaagaac ccaataaata ttgggtcctt ttatataatg ccttccatac tatcgaagaa
    13201 cttcacatat tatttctccg atttaacccc gaacaagtca taaattaatt tagaagtgtc
    13261 tgtaagtatt ttaatctctt cctttgaggt tgggtcaaac gacttcgcga agctaattaa
    13321 ttgtggtgca gcatctcgca ttttgcttaa aatatcagga tcgagcgttc cttcattcac
    13381 cagatgtgac atcttatcca gatagccatc aaaattcatt tctcccccat ctgccagacg
    13441 cttcatctct cccaggtact tcttcatatc acgttgatat aatttttcaa actgagcttt
    13501 caggtagctc tcattatttt cattaatata cactgtttct gataaatctg cgtaggcact
    13561 agaatatgaa agtgaaatta acagcgaagc aaataatttt aagctgttgt catcatgttc
    13621 ctggcaggca ttaacaaacg ttaaaaaacc agaacctatt gcttgaaaat gtatattcgc
    13681 taaaggttca ttatccttag attcctcata aaatagctga accgtggagg gaaggccatt
    13741 cttagtttct acatcaaaag tacattgttc aatgggcatt gcatctttat tatccccagg
    13801 attacatata ctattaatcg cgaaatgaaa aatagcccgg tcaacaactg atgcaataaa
    13861 gtcacgatcg tttaaatcct catcagtgca atcgtcagta tacttattga cccacatttt
    13921 gagatcattt atttgctcaa ttaaaaacac atttttttac gtgcatgcca taaattatgc
    13981 gtgattcgtt gaataaacaa atctattttt gcaataatta tatcccgatg acgcagcgct
    14041 gcataatcaa tagatttacc atatatatta tttaatttac ctttgattga acctctatta
    14101 tccaatttat ctttggttgc atctttattt tcgaattgat ttgaattgag tgttttatta
    14161 aagtatccag ttgcaccggg tattatcttc aacatgattc atctaccttt tttgtcgtac
    14221 cgtgtcctcc ttataattct tttgccatct ggtcactatc aaaaatcgct cataatttct
    14281 gaactacagg tatgcgttta aacccagtta aataaatagc gagttaactt gaagcaattt
    14341 atttaaaaat gaattattaa ttattatcgt gttgataatg gtacgacaag gcaacacaac
    14401 accttaccta caaaacagaa aatcaacatt acggcaacaa taagggttct cggtgttttt
    14461 cctggaacat ggtaaagtaa ggacattctt aacccccact ttgaggtgcc cgatggaaaa
    14521 gaccacaacg caggagttat tagcgcaggc tgaaaaaatc tgcgcgcagc gtaatgtgcg
    14581 cctgacccca cagcgcctgg aagtgttgcg cctgatgagt ctgcaggatg gcgctatcag
    14641 cgcttatgat ctgcttgatt tactgcgcga agctgaaccg caagccaagc cgccaacggt
    14701 ttatcgcgcg ctggattttc tgcttgagca aggttttgtg cataaggtgg aatccaccaa
    14761 cagttatgtg ctctgtcatc tgttcgacca gcccacgcat acgtcagcca tgtttatttg
    14821 cgatcgctgc ggcgcggtga aagaagagtg tgcagaaggc gtggaagaca ttatgcatac
    14881 gctggcggca aaaatggggt ttgccctgcg gcataatgtg attgaagcac atgggctctg
    14941 tgcggcatgc gtggaagtgg aagcgtgtcg tcatcctgaa cagtgccagc atgatcactc
    15001 tgtgcaggtg aaaaagaaac cgcgttaaga gggcgtacat ccttgtacac gtcgggcagg
    15061 agggattaat taccagcgat attcattgcg ggtttcccaa tccacgacct ctttttctgc
    15121 ctgatctttc tgataaccat aacgttcctg gattttaccg accagttgat cacgtttacc
    15181 ttcaatgatc gtcatatcat catcggtcag tttgccccat tgctctttca ctttaccttt
    15241 aaactgtttc cagttaccgc cggcttcatc tttattcata atcaagacct catcgttagg
    15301 ttgtgaatga gagtacgttc acttttcttc tgaacgtgag attaagtgta gtcagcaatt
    15361 tggcttagga taatttattc agaattttta accgtcacgt tgcggcaaac caggtaccgt
    15421 tgcgccagtg acgccgccag atagccgcca gagaaagccc gcgcaacgcc agaaagacgg
    15481 ttaatgccag ccacaaacca tgattaccca gccacggcag cgtaaggagc gtcagcgcaa
    15541 aacctgcggc ggccaccgcc atactgttac gcatttcggc ggcacgcgtt gcgcctataa
    15601 acatgccgtc cagcagataa caccagacgc caaccaacgg caaaatcacc tgccagataa
    15661 gatagcggtc agccagctgc tgaatctggg ttaacgacgt cagcaacgca atgatgtgtt
    15721 cccctgccag caaataaacc accgaaaaca gtaacgctac aatccccgac tggcggcacg
    15781 ctgcccgcca tacatccagc aactggctac cgtcgcgcgc accgtatgcc tgaccggagt
    15841 gcgcttcaac cgcgtaggca aaaccatcaa gcgcataggc ggtaaaggtg agtagcgtca
    15901 tcagaaccgc gttaacagcg ataatgtcac tccccagtcg cgcaccaagt acggtgatcg
    15961 cgccgaaaca gagttgcaac aacagcgaac gcagcatgat atcgcggtta agcgccagca
    16021 agcgacggaa gtttcctcgc caggcagttt tcagcatttc gccggaaatt ccgcgtagtt
    16081 tgaggatttt acgcaccatt agcagaccaa tcagcaatgt tgcatattcc gcaataaccg
    16141 tcgccagcgc cgcgccctgc acgttcatat gcagccccat caccagccag acatccagca
    16201 caatgttgag gatattgccg accactaaca aaattactgg cgcacgggca tattgcacgc
    16261 cgagtaacca accgagtaat accagattcg ccagcgacgc cggagcgctt aaccagcgga
    16321 tttcaagaaa gcgccgcgcc tgttccagga ccgcttcact accgccaaca atatgcagcg
    16381 ccagatcgat aatcggcgta cgcaacagcg caattaacgc cccggccccc aacgccaaca
    16441 gcaacggttg caccagcgca cgggctaatg cctgaggatt tttggcacca taagcctgcg
    16501 cagtcagccc ggtggtgctc atgcgtaaaa acagcaacag cataaagaga aagctggtcg
    16561 ccgttgcacc aaccgccacg ccgcccaaat aaaccggact atcaagatga ccaattaccg
    16621 ccgtatcgac cagtcccagc aacggaacgg tgatattgga gaaaatcatg ggtaaggcga
    16681 gatgccagag tgctttatca gatgaagtga ggaatgccat gcagacaagc ctgatgaaga
    16741 gagatgaaaa acaaaccgcg acgccaggcg gcatcgcggt ctcagagata tgttacagcc
    16801 agtcgccgtt gcgaataacc ccaaccgcca gcccttcaat ggtgaagctc tgctgacgaa
    16861 ggtcaacgac aattggttta aactcgctat tttctggcaa cagttcgact ttattgccct
    16921 gttttttcag gcgcttaacg gtaacttcgt catcaatacg tgcgacaacg acctgaccgt
    16981 tacgtacatc ctgagtttta tgcactgcca gcaagtcgcc atccataatg ccgatatctt
    17041 tcatcgacat cccgctgacg cgcagcagga aatcagcatt cggcttgaac aaggaaggat
    17101 cgacctgata atgaccttca atatgctgtt gcgccagaag cggttcaccg gcagccacac
    17161 gacctaccag cggcaaccct tcttcctctt cctgcaacag acgaatcccg cgtgatgcgc
    17221 cggaaacaat ttcaataacg cctttgcgtg ccagcgcctt cagatgttct tcagccgcgt
    17281 ttggggaacg gaaccccaaa cgctgcgcga tttccgcacg cgtcggcggc atacctgtct
    17341 ggctgatgtg atcacggatg agatcaaaca cctcttgttg cctggccgtt aacgctttca
    17401 ttccgccccc tgggtgtata tacagttata ctgtgagtat atacagcaaa aggcgatttt
    17461 ggaaccataa actgcacaat aaaccagaga tttatcgaaa tctggaaggg ttatccaaaa
    17521 tgcgaccata acaggatgca ccaggtaatc acggcgacga taatggcaat cagcaccgcg
    17581 gcggatccca tatcttttgc gcgtccggaa agctcatggt attcagagcc aattcggtca
    17641 accactgctt cgatggcgct attgaggatt tccacaatca tcaccagcat cacggagctg
    17701 ataagcagca cgcgggtaat tgcgtccaca tccagccagc aggcgatgac caccgccaac
    17761 aataccgcta cgccttcctg acggaatgcc gcttcgttga tccatgcagc gcgtaaacct
    17821 ttccaggaat agccagcagc tttgataatt cgggtgaatc cagtggtatt attggccatt
    17881 gaaagaacct ttttacatta tgagcgtcaa tatcagtgta ccgttaaatc cgatgattcg
    17941 ggtttgcgta acgccgcgcg aaacatgagc ggataccaca gaatttccca tgactttctg
    18001 ctatccttgc cgcgcatttg cattattaac cagaggcttt acatcgttta tgtccggctg
    18061 gccacgaatt tactacaaat tactgaattt accattaagc atcctggtaa aaagcaagtc
    18121 tattccggca gatcctgccc cggaactggg gctggatacc tctcgtccaa ttatgtacgt
    18181 tttaccgtac aactcgaaag cagatttgct gacgttgcgc gcccagtgtc tggcacatga
    18241 tttgcctgac ccgttagagc cgctggaaat cgacggcacg ctactgccgc gctatgtgtt
    18301 cattcacggc gggccgcgtg tattcaccta ttacacgccg aaagaagagt ctattaagct
    18361 tttccacgac tatctcgatt tgcaccgtag caacccaaat ctggatgtgc agatggtgcc
    18421 agtatcggtg atgtttggtc gcgcgccggg gcgtgaaaaa ggcgaagtga acccgccgct
    18481 gcgtatgctt aacggcgtac agaaattttt cgctgtactg tggctcggtc gcgacagttt
    18541 tgtgcgtttc tcgccgtcag tgtcgctgcg ccgtatggcg gatgaacacg gcacggataa
    18601 aactatcgct cagaaactgg cgcgcgtggc gcgtatgcac tttgcccgtc aacgtctggc
    18661 tgccgtaggc ccacgtctgc ctgctcgtca ggatctgttt aataagctgc tcgcctcccg
    18721 cgccattgcc aaagcggtag aagatgaagc gcgcagcaaa aaaatctccc atgaaaaagc
    18781 gcagcagaac gcgattgcgc tgatggaaga gattgcggcg aatttctctt acgagatgat
    18841 ccgcctgact gaccgcattc tgggcttcac ctggaaccga ttttatcagg gcatcaacgt
    18901 ccataacgcc gagcgcgttc gccagctggc ccacgacggt catgagctgg tgtatgtgcc
    18961 ttgccaccgc agtcacatgg actacctgct actttcttac gtgctgtacc accaggggct
    19021 ggtgccgccg catatcgccg ccgggatcaa cctgaacttc tggccggccg ggccgatttt
    19081 ccgccgtctg ggggcgttct ttattcgccg tacttttaaa ggcaataaac tttattccac
    19141 cgttttccgt gagtatctcg gcgaactgtt cagccgtggt tattccgtcg agtacttcgt
    19201 ggaaggcggt cgttcccgca cggggcgttt gctggatccg aaaaccggta cgctgtcgat
    19261 gaccattcag gcgatgctgc gtggcggcac gcgtccgatt acgctgattc cgatctatat
    19321 cggttatgag cacgtcatgg aagtgggtac ttacgccaaa gaactgcgcg gcgcgacgaa
    19381 agagaaagag agcctgccgc agatgctgcg cggtttaagc aagctgcgta atctcggtca
    19441 gggttacgtc aacttcggtg aaccaatgcc gttgatgacc taccttaacc agcacgtacc
    19501 agactggcgt gaatctatcg atcccatcga agcggtgcgt ccggcctggt taacgccgac
    19561 ggtcaataat attgctgccg atctgatggt acgcattaac aacgcaggcg cggcaaacgc
    19621 catgaacctg tgctgtactg cgctactggc atcacgtcag cgctcactca cccgcgagca
    19681 gttaaccgag caactcaact gctacctgga tctgatgcgc aacgtacctt actccacgga
    19741 ctctaccgtt ccttcagcca gcgccagcga gcttatcgat cacgcgctgc caatgaacaa
    19801 gtttgaagtc gagaaagaca ctatcggcga catcatcatt ctgccacgcg agcaagcggt
    19861 gctgatgacc tactatcgca acaacattgc gcatatgttg gtgctgcctt cgctgatggc
    19921 ggcaattgtc acccagcatc gccacatctc ccgcgacgta ttgatggagc acgtcaatgt
    19981 gctttaccca atgctgaaag cggagctgtt cctgcgctgg gatcgcgacg agttgccgga
    20041 cgttattgat gcgctggcaa atgagatgca acgtcagggg ctgattaccc tgcaagatga
    20101 tgagttgcat atcaacccgg cgcattctcg caccctacag ctgctggccg caggcgcgcg
    20161 cgaaacgctg caacgttatg ccatcacctt ctggttgttg agtgccaacc cgtcgatcaa
    20221 ccgcggtacg ctggagaaag agagccgcac cgttgcgcaa cgtctctccg tgctgcacgg
    20281 catcaacgcg ccggagttct tcgacaaggc ggtgttcagt tctctggtgc tgacgctgcg
    20341 tgatgaaggg tatatcagcg atagcggcga tgccgaacca gcagaaacga tgaaggttta
    20401 tcagttgctg gcggagttga ttacatcaga cgtgcgtttg acgattgaga gtgcgacgca
    20461 gggcgaaggg taatcagaga gaattgccgg atgcggcgaa aacgccttat ccggcctacc
    20521 atgacctgca aattcaataa attgcgattc accgggtagg cctgagaagc gcagcgcatc
    20581 aggcaaccca gaagaaagcc ggatgatcat ccggcttttt tacatcatca gaaatgccag
    20641 taactcattg ccagtcctaa aaacagtacc agaccaacat agttattatt cataaatgct
    20701 ttaaagcagg cttcacgctc gcggttggca atcagttttt gttgataaac aaacagcgcg
    20761 ccagccacca caattgacca gtaatatccc cagcctaagc catttaactc accgatgatc
    20821 gccatcagtg ccagtacgcc aatctgcaaa ataccaataa tcaatttatc gtattggccg
    20881 aacaggattg ccgtggattt aatgccaatc ttcacatcat catcgcggtc aaccatcgca
    20941 tactgcgtgt cgtaagccac cgcccagaga atattggcga ggaacattaa ccagcaactc
    21001 aatggcaccg actcactcac agcggcaaaa gccattggaa tcgaccagcc aaacgccgcg
    21061 cccagcacca cttgcgggag atgggtatac cgcttcataa acgggtacac ccacgccaat
    21121 gccagagcgg caatcgacaa cagaatggtc atcgtattca gcgtcagaac cagtaaaaac
    21181 gaaatcagta ccagcactac aaacagcgcg cgcgcctctt tctctgttac cgcgccgctg
    21241 ggaagtggtc ggttcgccgt gcgcttaaca tgaccatcaa acttgcgatc agcataatca
    21301 ttcaccacgc aaccagcagc gcgcatcagc cagacacccg cgacaaacac cgccaggatc
    21361 cagagctgag gaacgcccgg tgtcgccacc cacaacgccc ataatgttgg ccagagcagc
    21421 aataacgcgc caattggctt atccgtacgc attaagcgat gaaacgccag cagcttattc
    21481 tgcgtcagac tccactccat atttttttcc tcttagtaca acggtgacgc cggtaaaaac
    21541 agttctgtta gcaacagcgg tttaccgctt aatcgcaggc gggaacgtcg cccccacagt
    21601 ccggcatcac ggcctatctc gataaaatcc cgggttagtg tcgatgatgt gaacagatag
    21661 cgtcctaacg gcgttttacc caatttttgt aacgctagtt ccggcccgct taatgttgat
    21721 acaggaacga cggtacgacc ggcaagccac ggttcaccat cggcacataa caaaatttca
    21781 cgtaaccagt aacgagactc tttcggcagt agcggcagtt cttcggggat ttcattctgc
    21841 tcgacaaacc cctcgcggat catcgtcaca ctcaccgttt ttccctgctg ttcaaaacgt
    21901 tttgtcatgg aatcctccag caacagccag tcgaacagtt gcggatccag ggcagggatc
    21961 tctttaaaat agcgcagcgc acgcagttgc gttaacgcgg ggtgtgacat gccgaactct
    22021 ccgttacata acgtaaaggc attgtatcgc agaaaagtgt ttacgggaga tgtaaagcaa
    22081 attaagtgat ctaatgcgca acaaaaacgc aacaatcgca tgaagtggta aaacgctgtt
    22141 gaaccttacg ctttgccgat gcccgtgctg gcgagggcaa ccataggggc atcaccgccc
    22201 atgatgctag gaacggtgat gttttccttt acttaaatac ctttattctt tatacgagta
    22261 gctcgccaac tcgcagtaaa aattgggaaa agcgcatatt taccatgaga tttggagctg
    22321 aaaagtgttt cctgagtaag attaataggc aacatcttat gtctggattt atagatacac
    22381 gtggaattcc aattggtatc ttttgtagga ttcaacatat catgtaagcg ctgggagctt
    22441 accaccataa caggcccatc atcgacagag gagaacagga taaatatatt atcgtttcgt
    22501 gttaatttat tagtgttaaa ggttcttaac ttttcttcag tttctataaa tataggtgaa
    22561 acatcatcac tacgaatcca ctctttataa agataccagg catttgcttt gatcatatca
    22621 tccgttgatc gtaaggcctg atcgataagt tggataaaaa aattgttatt tttatcaacc
    22681 agccctttat tcatattaaa tctgttaata aaatggatca tctgttgggt atcgaaattc
    22741 accttactga atcgatgttg acgcataaaa ttatcgaact tgtcatcttc cccagaatgt
    22801 atataactac gtataattgg atcttttata taaggacaat ggctaaatgt tttcttcata
    22861 ttctcaataa agtttttatt aacaatattg agaattttta attgcatcac cagttttact
    22921 gccagaacgg atttcaatat cattgcatta tctggcaatg tatcaatcat ttttataatg
    22981 ttattgatat aaccatcgag atcttcattc ccaaatatat ctgaataatc aatagttaat
    23041 tccaattgtt ctgtaatgca atcttaaaaa ttccctgtcg acattcgcac atgatcaaac
    23101 ataacagctg tcaaattcga tttaataaat gatatttctt tggcagacag aaactctgct
    23161 ctggtgaagt gcgaagcgta aagataacaa ttatcgaagt ttgaatttct aatctgacat
    23221 tcgcgaaaat tactatgacc aagttgactc tgcgagaagt caactgtatc aagaatattt
    23281 tggctaaaat caacacattc gccactcgtt ttgagaaaaa tcacattgtg taaacgtcga
    23341 catttaaata tgacattatg aactgttgcc ttagtaaaat tgcaattctc aatttcttta
    23401 cattcgaaag ttacatgttg tagatttgat gataaaaaag acaccttaga aataacgtca
    23461 cctttaaaat gacaatttgt taaatcaaaa tcagacaggt cgatataccc ctgcgcatct
    23521 ttatccatca gaaaattaca agcgcattct attttttcat ttctgtagaa ttttacactt
    23581 atagtatcat tactaattga gacttcacgt aacatattta ttgcatgatt tttttcgttc
    23641 cgttgtttta tatttaaaaa tatatcactg atataaataa agttatctct gtttaaacct
    23701 gtcaccccat tgaaattacg tagtaaatca agatctacta tattatttcc ttgcaccaca
    23761 tcatgtttca tcaaagagtt attgccgcca gaatttgtga tatgacctat cataaaacct
    23821 ctgtaataca gaaataaaat aacctggaaa agtagcctga gcgtccagac actaccggcg
    23881 aaaggaaaag aatctctgat aatgcattga aataatggac attcttagtg aagaagaagt
    23941 gaagtactat aaaaaatcgc tcatatccca ggaaggaagc ataaaaaaag gtgcgccagg
    24001 agacgcacca gttgttgcaa aatcagcact gtggggcgta attacccctt gccttttaca
    24061 ctgctgataa aggtggaacg ggcagatgtc gatcccaggc gttcagcttc atcaagcagt
    24121 tttaacgcct tatcaacatc acctttcgcg acagcgtttt tgatcgcggt attaaaataa
    24181 ctttccgtgt cgttgagcat cggctcgctt ttcttcaccg gcaccggagc aggtgcagcc
    24241 acagctggtg ccgccgtgtt gcctaccgta accggagctg gagcggaaga accaaatagt
    24301 ggtcctacca acacgctgga gctggagttc gttttgactt tcagtttcag taagccatcg
    24361 ctggtatgac gagcaaccgg atcgggaata tccgggatcg agttaccgac gcctttggca
    24421 taggctttag ccgggtcgag cagttgggtc gtctgctgga gatctttttc catggtaaag
    24481 accagaacat aaagtttttg ctgccccaac gccggtgtca ggcgcataac gccttccagc
    24541 cgatctgcac tcatcacgcc tagttcctgg taggtgaaat aactgctggg gaagaaggct
    24601 gatggggtca tgttctgatc aataatcagc acgttcggcg caaaaacgct ggtttgtttg
    24661 ttcacttcgc tggtcagcgt cagggtcagt tcgccaatgt ttgccgggac gctgtacgca
    24721 gcaaccggac cactgatgcc gggaacgctc agttgttggc cgccggtcgc cagttgggtg
    24781 gtctgggttt tagattgatc gaccggtgtc caggtgagtt gttgcagcgc ggcagatgga
    24841 atggctggcg cgtcgctggt gttttgcggt acgtagttaa catcggcaag gctaattcca
    24901 ggcgcgcttg ccagtaaccc tgctgataaa cagagggcga tgagactttt attcattttc
    24961 attgttttca cctcaaaatc tggagctcag cggtagccag gcaatagcgc gctaaacccg
    25021 ataatcagag gggctttcgc ccctttcaga taatgacaac ctgtttttat gccggatgcg
    25081 gcgtaaacgc cttatccggc ccaggttttg ctattaccac cagatttcca tctgggcacc
    25141 gaaggtccac tcgtcgctgt cgccacgacc gaagctgccg ccgttgaaat cagcaggaac
    25201 ggctttgccg tagttcgggt taaccttgct atcgccgttg tagtcgtaac cccatttctc
    25261 atcccacttg gcgtaggttg cgaagacacg aatagccggg cgtgaccaga tgctgtcgcc
    25321 agcctgccat tgttgtgcga gggtaatttt gtactgattg ttcttgtcgc cggtgcgctg
    25381 ggattcgacg ttgtcgtagc cgatttccat cacggtgctc atgattggcg tccacttgta
    25441 catcgggcga ataccaacgg tccaccactt ggtgccgttg tcgttatccc agttgatatc
    25501 ctggtacata cccacgtaca tcatgtccca gttgtcgccc atggagatcg caccgtggtc
    25561 gaggatacgc agcatgtgac cgttgttgtt gatattgtag gcaaattttt cgttatcaaa
    25621 cgcgacgcca gaaccctgcg acagaccttt accctgcgag gtcatcgagt cagtagcgta
    25681 ctgaacaaca aacttgttaa agcccttcag gacactctga gtatgttcag cagtgaataa
    25741 ccagccgtct ttcgatgcgc catcaaccag acgatagtta tcacgcaggt tggcacgacc
    25801 gtagtcgaca cccagttcta atgtgccgcc cgggttgatt tccatctgcg ctaaacgcac
    25861 atcgaaaacg tcgttcgcgg tttcgttggt atagtcataa atattgttgc tggcaaaaga
    25921 ggaagaacca ccagcttcag aggagcgggt tgctgccaga gagagtttac cgaagccaac
    25981 atcgatgttt tccagaccgg caccaggacc agaaatatcc cagtagtaga agtcgatcat
    26041 atgaacgtca tgacgttggt agaagcgctt acctgcccag atggtggaac ctggcagcca
    26101 ttcgatcagg ttttttccct gcacgtttgc ttcacggaag gccggatcgg tagcttccca
    26161 gtcattctgt tgcgcgacgg aataggccac gttagtgtcg aaatagaagc tcttatcgcc
    26221 ctctttccac acttcctgac ccaattttaa ttcagcataa gtttcacatt cgttgccaag
    26281 acggtattta ctttgagcac cggtagtctg gaaacactgt tgttcaccgc cgctacctgt
    26341 ccagccaata ccggaacgtg catagccgtg gaaatcaaca gccattgcct gagcagacat
    26401 tacgcccgct gcgacggcaa ccgccagagg aagtttgcgc agagtaatca tcattctatc
    26461 tcctgagtca ttgcttttct tttttcacat cacctgtgac aggctttgtg tgttttgtgg
    26521 ggtgcttaaa cacccggctc cttatgcagt cgacgacatg cagtgccatc ctcacggaac
    26581 agatggcaac gctctggcgg caggccgata gcgaatgtgg caccttcttc taccaacacc
    26641 acgtcgttct ggcggtacac caggttttga cgaatggaag ggatctggat atagatttgg
    26701 gtttcgttgc cgagttgctc gacgacctga acttcaccct caaggatgac gtcagcgata
    26761 tcactcggca gtagatgttc cgggcgaata cccagcgaca tattggctcc aacctggaca
    26821 tcacggcttt caacaggcag ccagacttgt tgacgatttg gcatcggcag ctccacctgc
    26881 acttgatcga ttgcggtggc ggtcactttt accggcagga agttcatctt tggcgaaccg
    26941 ataaatccgg cgacaaaacg gtctgccgga tagtggtaca gttccagcgg tttcccaacc
    27001 tgtgccacgc gaccggcgtc cagcaccacg attttgtcgg ccagcgtcat cgcttcgacc
    27061 tgatcgtggg tgacgtaaat cattgtgcgg cccaggcgtt tatgcagacg ggagatttcg
    27121 atacgcattt gcacacgcag tgcagcatcg aggttggaga gcggttcatc gagcaaaaat
    27181 acgcttggct cggccaccag cgtacggcca atcgccacgc gctgacgctg accaccggag
    27241 agcgctttcg gtttgcgatc cagcaaatgc gccagttgca gcacttccgc cacctggttg
    27301 acgcgttggt taatcacctc tttttttgcg ccagccagtt tcaggccaaa tgacatgttt
    27361 tctgctactg acaggtgggg atagagcgcg taagactgaa acaccatacc aacgccgcgt
    27421 tctgctggcg gagtgtcatt catccgtttc tcaccgatga acaggtcgcc gctggtgatc
    27481 gtctcaagcc cggcaatcat gcgcagtaaa gtcgatttac cgcagccaga cggtccgaca
    27541 aacaccacga attcaccttc atggatatcg agattgatat ctttcgatac cacgacctcg
    27601 ccccaggctt tcgttacatt ttgcagctgt acgctcgcca tgcccttctc cctttgtaac
    27661 aacctgtcat cgacagcaac attcatgatg ggctgactat gcgtcatcag gagatggctt
    27721 aaatcctcca ccccctggct tttttatggg ggaggaggcg ggaggatgag aacgcggctt
    27781 ctgtgaacta aaccgaggtc atgtaaggaa tttcgtgatg ttgcttgcaa aaatcgtggc
    27841 gattttatgt gcgcatcccc acattaccgc caattctgta acagagatca cacaaagcaa
    27901 cggtggggcg taggggcaag gaggatggaa agaggttgcc gtataaagaa actagagtcc
    27961 gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa aataaaaaca
    28021 ggtgcacgca tcctcgcatt atccgcatta acgacgatga tgttttccgc ctcggctctc
    28081 gccaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt
    28141 ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat
    28201 ccggataaac tggaagagaa attccctcag gttgcggcaa ctggcgatgg ccctgacatt
    28261 atcttctggg cgcacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc
    28321 accccggaca aagcgttcca ggacaagctg tatccgttta catgggatgc cgtacgttac
    28381 aacggcaagc tgattgctta cccaatcgct gttgaagcgt tatcgctgat ttataacaaa
    28441 gacctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
    28501 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
    28561 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa
    28621 gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt
    28681 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
    28741 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
    28801 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatctaa accgttcgtt
    28861 ggcgtgctga gcaccggtat taacgccgcc agtccgaaca aagagctggc gaaagagttc
    28921 ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg
    28981 ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
    29041 accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
    29101 tggtatgccg tacgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
    29161 gccctgaaag acgcgcagac tcgtatcacc aagtaatgct gtgaaatgcc ggatgcggcg
    29221 tgaacgcctt gtccggccta caaaactgaa acgcatgtag gcctgataag acgcatcagt
    29281 gtcgcatcag gcagttgttg tcggataagg cgtgaaagcc ttatccgtcc tggaatgagg
    29341 aagaacccca tggatgtcat taaaaagaaa cattggtggc aaagcgacgc gctgaaatgg
    29401 tcagtgccag gtctgctcgg cctgctggtg ggttaccttg ttgttttaat gtacgcacaa
    29461 ggggaatacc tgttcgccat taccacgctg atattgagtt cagcggggct gtatattttc
    29521 gccaatcgta aagcctacgc ctggcgctat gtttacccgg gaatggctgg aatgggatta
    29581 ttcgtcctct tccctctggt ctgcaccatc gccattgcct tcaccaacta cagcagcact
    29641 aaccagctga cttttgaacg tgcgcaggaa gtgttgttag atcgctcctg gcaagcaggc
    29701 aaaacctata actttggtct ttacccggcg ggcgatgagt ggcaactggc gctcagcgac
    29761 ggcgaaaccg gcaaaaatta cctctccgac gcgtttaaat ttggcggcga gcaaaaactg
    29821 caactgaaag aaacgaccgc ccagcccgaa ggcgaacgcg cgaatctgcg cgtgattacc
    29881 cagaatcgtc aggcgctgag tgacattacc accattctgc cggatggcaa caaagtgatg
    29941 atgagctccc tgcgccagtt ttctggcacg cagccgctct acacactcga cggtgacggc
    30001 acgttgacga ataatcagag tggcgtgaaa tatcgtccga ataaccaaat tggcttttac
    30061 cagtccatta ccgccgacgg caactggggg gatgaaaagc taagccccgg ttacaccgtg
    30121 accaccggct ggaaaaactt tacccgcgtc tttaccgacg aaggcattca gaaaccgttc
    30181 ctcgccattt tcgtctggac cgtggtgttc tcgctgatca ctgtcttttt aacggtggcg
    30241 gtcggcatgg ttctggcatg tctggtgcag tgggaagcgt tgcgcggcaa agcggtctat
    30301 cgcgtcctgc tgattctgcc ctacgcggtg ccatcgttca tttcaatctt gattttcaaa
    30361 ggattgttta accagagctt cggtgaaatc aacatgatgt tgagcgcgct gtttggcgtg
    30421 aagcccgcct ggttcagcga tccgaccacc gctcgcacga tgctgattat cgtcaatacc
    30481 tggctgggtt atccgtacat gatgatcctc tgcatgggct tgctgaaagc gattccggac
    30541 gatttgtatg aagcctcagc aatggatggc gcaggtccgt tccagaactt ctttaagatt
    30601 acgctgccgc tgctgattaa accgctgacg ccgctgatga tcgccagctt cgcctttaac
    30661 tttaacaact tcgtgctgat tcagctgtta accaacggcg gcccggatcg tcttggcacg
    30721 accacgccag ccggttatac cgacctgctt gttaactaca cctaccgtat cgcttttgaa
    30781 ggcggcgggg gtcaggactt cggtctggcg gcagcaattg ccacgctgat cttcctgctg
    30841 gtgggtgcgc tggcgatagt gaacctgaaa gccacgcgaa tgaagtttga ttaagggaga
    30901 taacaaaaat ggcaatggtc caaccgaaat cgcaaaaagc acgtttgttt attactcacc
    30961 tgctactgct actttttatc gcggcgatta tgttcccgct gctgatggtc gtcgctatct
    31021 cgctgcgtca gggaaacttc gcgaccggta gcctgatccc ggagcaaatc tcctgggatc
    31081 actggaaact ggcgttaggt tttagcgttg aacaggctga tggtcgcatt acgccaccgc
    31141 cattcccggt actgctgtgg ctgtggaact cggtaaaggt cgctgggatt tccgcgattg
    31201 gcattgtggc gctctccacc acctgcgcct acgctttcgc ccgtatgcgc tttccaggca
    31261 aagcgacgct gctgaaagga atgctgattt tccagatgtt cccggcagta ctttcactgg
    31321 tcgcgttgta tgcgttgttt gatcgtctgg gtgagtacat tccattcatt ggcctgaata
    31381 ctcacggcgg cgtaattttc gcgtatctgg gtgggattgc gctgcatgtc tggaccatca
    31441 aaggctattt cgaaaccatc gacagttcgc tggaagaagc ggctgcgctg gatggtgcga
    31501 caccctggca ggccttccgc cttgtcctgt tgccgctgtc agtaccgatt ctggcggtgg
    31561 tattcatcct gtcgtttatc gctgccatta ctgaagttcc ggtcgcgtcg ctgttactgc
    31621 gtgacgtaaa cagctacacc ctggccgtgg ggatgcagca atacctcaac ccgcaaaact
    31681 acctgtgggg tgactttgcc gccgctgccg tgatgtctgc attaccgatc accatcgtct
    31741 tcttgctggc tcaacgctgg ctggtcaacg gcctgacggc aggtggtgtg aaag
//
LOCUS       DAJTYI010000036        30372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYI010000036 DAJTYI010000000
VERSION     DAJTYI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OO564_001589"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OO564_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6652750.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="OO564_001590"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="OO564_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6652751.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="OO564_001591"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="OO564_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6652752.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="OO564_001592"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="OO564_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6652753.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="OO564_001593"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="OO564_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6652754.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="OO564_001594"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="OO564_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6652755.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="OO564_001595"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="OO564_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6652756.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="OO564_001596"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="OO564_001596"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6652757.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="OO564_001597"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="OO564_001597"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6652758.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OO564_001598"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OO564_001598"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6652759.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OO564_001599"
     CDS             complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OO564_001599"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6652760.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OO564_001600"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OO564_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6652761.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OO564_001601"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OO564_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6652762.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OO564_001602"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OO564_001602"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6652763.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OO564_001603"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OO564_001603"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6652764.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OO564_001604"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OO564_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6652765.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="OO564_001605"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="OO564_001605"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6652766.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="OO564_001606"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="OO564_001606"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6652767.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="OO564_001607"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="OO564_001607"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6652768.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="OO564_001608"
     CDS             21982..22572
                     /locus_tag="OO564_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6652769.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="OO564_001609"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="OO564_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6652770.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="OO564_001610"
     CDS             23044..23739
                     /locus_tag="OO564_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6652771.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OO564_001611"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OO564_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6652772.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="OO564_001612"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="OO564_001612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6652773.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="OO564_001613"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="OO564_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6652774.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OO564_001614"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OO564_001614"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6652775.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OO564_001615"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OO564_001615"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6652776.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7111 a         7914 c         8360 g         6987 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag gaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac tggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgaaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagaaccggc
    12061 aatcttggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagttgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cacaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatctgcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggtg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatgag cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gagttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgctgg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgactc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatccct
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa ga
//
LOCUS       DAJTYI010000037        29744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYI010000037 DAJTYI010000000
VERSION     DAJTYI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            38..1486
                     /gene="uacT"
                     /locus_tag="OO564_001616"
     CDS             38..1486
                     /gene="uacT"
                     /locus_tag="OO564_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6652777.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1488..1613
                     /locus_tag="OO564_001617"
     CDS             1488..1613
                     /locus_tag="OO564_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652778.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1610..1681)
                     /locus_tag="OO564_001618"
     CDS             complement(1610..1681)
                     /locus_tag="OO564_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652779.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1736..2284
                     /gene="idi"
                     /locus_tag="OO564_001619"
     CDS             1736..2284
                     /gene="idi"
                     /locus_tag="OO564_001619"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6652780.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2327..3844)
                     /gene="lysS"
                     /locus_tag="OO564_001620"
     CDS             complement(2327..3844)
                     /gene="lysS"
                     /locus_tag="OO564_001620"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6652781.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(3854..4952)
                     /gene="prfB"
                     /locus_tag="OO564_001621"
     CDS             complement(join(3854..4876,4878..4952))
                     /gene="prfB"
                     /locus_tag="OO564_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6652782.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5043..6776)
                     /gene="recJ"
                     /locus_tag="OO564_001622"
     CDS             complement(5043..6776)
                     /gene="recJ"
                     /locus_tag="OO564_001622"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6652783.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6782..7492)
                     /gene="dsbC"
                     /locus_tag="OO564_001623"
     CDS             complement(6782..7492)
                     /gene="dsbC"
                     /locus_tag="OO564_001623"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6652784.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7517..8413)
                     /gene="xerD"
                     /locus_tag="OO564_001624"
     CDS             complement(7517..8413)
                     /gene="xerD"
                     /locus_tag="OO564_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6652785.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8525..9046
                     /gene="fldB"
                     /locus_tag="OO564_001625"
     CDS             8525..9046
                     /gene="fldB"
                     /locus_tag="OO564_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6652786.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9086..9493)
                     /locus_tag="OO564_001626"
     CDS             complement(9086..9493)
                     /locus_tag="OO564_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6652787.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9474..9740)
                     /gene="sdhE"
                     /locus_tag="OO564_001627"
     CDS             complement(9474..9740)
                     /gene="sdhE"
                     /locus_tag="OO564_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6652788.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            9983..10963
                     /gene="ygfZ"
                     /locus_tag="OO564_001628"
     CDS             9983..10963
                     /gene="ygfZ"
                     /locus_tag="OO564_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6652789.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11159..11818)
                     /locus_tag="OO564_001629"
     CDS             complement(11159..11818)
                     /locus_tag="OO564_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6652790.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(11982..12293)
                     /locus_tag="OO564_001630"
     CDS             complement(11982..12293)
                     /locus_tag="OO564_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6652791.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12338..13770
                     /gene="bglA"
                     /locus_tag="OO564_001631"
                     /pseudo
     CDS             12338..13770
                     /gene="bglA"
                     /locus_tag="OO564_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(13827..14570)
                     /locus_tag="OO564_001632"
     CDS             complement(13827..14570)
                     /locus_tag="OO564_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6652792.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(14837..17710)
                     /gene="gcvP"
                     /locus_tag="OO564_001633"
     CDS             complement(14837..17710)
                     /gene="gcvP"
                     /locus_tag="OO564_001633"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6652793.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(17829..18218)
                     /gene="gcvH"
                     /locus_tag="OO564_001634"
     CDS             complement(17829..18218)
                     /gene="gcvH"
                     /locus_tag="OO564_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6652794.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18242..19336)
                     /gene="gcvT"
                     /locus_tag="OO564_001635"
     CDS             complement(18242..19336)
                     /gene="gcvT"
                     /locus_tag="OO564_001635"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6652795.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19627..19710
                     /locus_tag="OO564_001636"
     CDS             19627..19710
                     /locus_tag="OO564_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652796.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19784..20986)
                     /gene="ubiI"
                     /locus_tag="OO564_001637"
                     /gene_synonym="visC"
     CDS             complement(19784..20986)
                     /gene="ubiI"
                     /locus_tag="OO564_001637"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6652797.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21009..22187)
                     /gene="ubiH"
                     /locus_tag="OO564_001638"
                     /gene_synonym="visB"
     CDS             complement(21009..22187)
                     /gene="ubiH"
                     /locus_tag="OO564_001638"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6652798.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22184..23509)
                     /gene="pepP"
                     /locus_tag="OO564_001639"
     CDS             complement(22184..23509)
                     /gene="pepP"
                     /locus_tag="OO564_001639"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6652799.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23535..24113)
                     /gene="ygfB"
                     /locus_tag="OO564_001640"
     CDS             complement(23535..24113)
                     /gene="ygfB"
                     /locus_tag="OO564_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6652800.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24281..24610
                     /gene="zapA"
                     /locus_tag="OO564_001641"
     CDS             24281..24610
                     /gene="zapA"
                     /locus_tag="OO564_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6652801.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24652..24835
                     /gene="ssrS"
                     /locus_tag="OO564_001642"
     ncRNA           24652..24835
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO564_001642"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            24856..25458
                     /locus_tag="OO564_001643"
     CDS             24856..25458
                     /locus_tag="OO564_001643"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6652802.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25555..25614)
                     /locus_tag="OO564_001644"
     CDS             complement(25555..25614)
                     /locus_tag="OO564_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6652803.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(25947..26006)
                     /gene="ibsC"
                     /locus_tag="OO564_001645"
     CDS             complement(25947..26006)
                     /gene="ibsC"
                     /locus_tag="OO564_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6652804.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26235..27467)
                     /gene="serA"
                     /locus_tag="OO564_001646"
     CDS             complement(26235..27467)
                     /gene="serA"
                     /locus_tag="OO564_001646"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6652805.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27723..28382)
                     /gene="rpiA"
                     /locus_tag="OO564_001647"
     CDS             complement(27723..28382)
                     /gene="rpiA"
                     /locus_tag="OO564_001647"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6652806.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28765..29658
                     /gene="argP"
                     /locus_tag="OO564_001648"
     CDS             28765..29658
                     /gene="argP"
                     /locus_tag="OO564_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6652807.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7059 a         8020 c         7472 g         7193 t
ORIGIN      
        1 ttgcttttta ttctggcggg cgaaaggaga aacactgatg agcgccatag attcccaact
       61 tccctcatct tctgggcaag accgcccaac tgatgaggtt gaccgcatat tatcaccagg
      121 aaagctgatc atactcggtc tgcaacacgt ccttgtcatg tacgcaggtg cagtcgctgt
      181 tcctcttatg attggtgacc gactgggcct ctcaaaagat gctattgcga tgctcattag
      241 ctcggatctc ttttgctgcg ggatcgtcac attattgcaa tgtatcggta tcggccgctt
      301 tatggggatc cgcctgccgg tgattatgtc ggtgaccttt gctgctgtaa caccaatgat
      361 agccattggg atgaacccgg atatcggcct gctggggata tttggtgcca ctatcgccgc
      421 gggttttatc accacattat tagcgccact tattggtcgc ttgatgcctt tattcccgcc
      481 actggttacc ggtgtggtta ttacttctat tgggcttagc atcattcagg tgggtattga
      541 ctgggccgct ggaggtaaag ggaatccgca atatggtaat cccgtttatt taggtatctc
      601 ctttgccgtc ttaattttta tcttgctcat tactcgctat gcgaaaggat ttatgtccaa
      661 cgtcgccgta ttactgggga ttgtatttgg ctttttactt tcgtggatga tgaatgaagt
      721 caatttatcc gggctacatg atgcctcgtg gtttgcgatt gtcaccccga tgtcgtttgg
      781 tatgccgatt ttcgatcccg tttccattct gaccatgact gccgtgttaa tcatcgtgtt
      841 tatcgagtcg atggggatgt tcctggcact gggtgaaata gtcggtcgta aactctcttc
      901 acacgatatt attcgcgggc tgcgtgtcga tggcgtaggg acaatgatag gcggcacgtt
      961 taacagcttc ccccacacgt cattttctca aaacgttggc ctggttagcg tgatgcgcgt
     1021 tcatagccgc tgggtgtgta tttcttcggg aattatatta atcctgtttg gcatggtgcc
     1081 aaaaatggcg gtgctggtag cctccattcc gcaatttgtg ctgggcggcg ctggtctagt
     1141 gatgttcggc atggtactgg cgacagggat tcgaattctg tcgcgctgta actacaccac
     1201 caaccgttac aacctctata ttgtggcgat cagtctcggc gttggcatga ctccgacgct
     1261 ctctcacgat ttcttttcta agttaccggc cgtactgcaa ccgctgctac atagcggcat
     1321 tatgctcgca acccttagcg ccgttgtgct gaacgtcttc tttaatggct atcagcatca
     1381 tgctgacctg gtgaaggaat ccgtctctga taaagattta aaagtcagga cagtacgtat
     1441 gtggcttctg atgcgcaagc tgaagaaaaa tgagcatgga gaataatatg aattttttaa
     1501 tgcgcgctat attcagtctg ttgttgcttt ttactctctc tattcctgtc atttctgact
     1561 gtgttgcaat ggccattgaa agtcgcttca aatatatgat gctacttttt taaatggttt
     1621 ttacctgtcg gcatccgctc aaaacgggcg gttgtcgata aacgctctct tggttaatca
     1681 tttcactctt caattatcta taatgatgag tgatcagaat tacatgtgag aaattatgca
     1741 aacggaacac gtcattttat tgaatgcaca gggagttccc acgggtacgc tggaaaagta
     1801 tgccgcacac acggcagaca ccctcttaca tctcgcgttc tccagttggc tgtttaatgc
     1861 caaggggcaa ttattagtta cccgccgcgc actgagcaaa aaagcgtggc ctggcgtgtg
     1921 gactaactcg gtttgtgggc acccacaact gggagaaagc agcgaagacg cggtgatccg
     1981 ccgttgccgt tatgagcttg gcgtggaaat tacgcctcct gaatctatct atcctgactt
     2041 tcgctaccgc gccaccgatc cgagtggcat tgtggaaaat gaagtgtgtc cggtatttgc
     2101 cgcacgcacc accagtgcgt tacagatcaa cgatgatgaa gtgatggatt atcaatggtg
     2161 tgatttagct gatgtattac gcggtattga tgccacgccg tgggcgttca gtccgtggat
     2221 ggtgatgcag gcgacaaatc gcgaagccag aatacgatta tctgcattta cccagcttaa
     2281 ataaaaaacc ccgacatttg ccggggttgt gagcataacg taatgcttat ttcaccggac
     2341 gcatcgccgg gaacagaata acgtcgcgga tggtatggct gttggtgaac agcattacca
     2401 tacggtcgat gccaatcccc agacctgctg tcggcggtaa gccatgttcc agtgcggtaa
     2461 cgtaatcttc atcgtagaac atcgcttcgt cgtcacctgc gtctttcgcg gcaacctgat
     2521 ccaggaagcg ttgcgcctga tcttccgcgt cattcagctc gctaaagccg ttaccgattt
     2581 cacgaccacc gatgaagaac tcaaagcggt ctgtgatttc cgggttaatg tcgttacgac
     2641 gcgccagcgg agaaacttct gccggatatt cagtaatgaa ggtcggctga atcagatgtg
     2701 cttctgccac ttcttcgaag atctcggtaa cgatacggcc cagaccccag ctcttctcaa
     2761 cgtggatacc gatagattca gcaatcgctt tcgcagagtc gaagttatcc agatccgcca
     2821 tgtcggtttc cgggcgatat ttcttgatcg cttcacgcat ggtcagtttt tcgaacggtt
     2881 taccgaagtc cagcgtcacg tcgccgtagg tcacttccgt cttaccgaga atatcctgtg
     2941 ccagagtacg gaacagcgat tcggtcagct cgatcagatc tttgtaatct gcgtaagcca
     3001 tgtagagttc catcatggtg aactctgggt tatgacgtac ggaaatacct tcgttacgga
     3061 agttacggtt gatttcgaat acacgctcga agccaccgac caccagacgc ttgaggtaca
     3121 gttccggcgc gatacgcagg tacatgtcga gatccagcgc gttatggtgg gtgataaacg
     3181 gacgcgcagc ggcaccgcct gggatcacct gcatcatcgg cgtttcaact tccataaagc
     3241 cacggttcac catgaactgg cgaataccag agaggatctg cgagcgtact ttaaaggtgt
     3301 tacgggattc atcgttagag atgagatcca gataacgctg acgatagcgc gcttcctgat
     3361 cctgcaagcc gtggaattta tccggcagcg gacgcagtgc tttggtcagc agacgcagct
     3421 cggtacagtg gatagacagt tcgccggttt tggttttgaa cagtttacct ttcgcgccga
     3481 ggatgtcgcc gaggtcccat tttttgaact gctcgttgta aacgccttcc ggcagatcgt
     3541 cacgggcaac gtacagctga atgcggccac ccacatcctg cagggtaacg aaagacgctt
     3601 tacccataat acggcgggtc atcatgcggc cagcaacggc gacttcgatg ttcagcgctt
     3661 ccagttcttc gttctcttta ccgtcgaatt ctgcgtgcaa ttggtcagag gtatgatcgc
     3721 gacggaaatc gttcgggaag gcaattccct gctcacgcag gttcgccagc ttctcacgac
     3781 gcgttttcag ttcattgtta agatcgacta ccgcgtcagc gccctgtgcg tgttgttcag
     3841 acatgttggt tcctcataac cctgctttca aacttgcttc gataaattga tccaggctgc
     3901 cgtccagcac ggcctgcgtg ttgcgagttt ctaccccggt gcgcagatct ttaatgcggg
     3961 agtcatcaag gacataagaa cgaatctggc tgccccagcc gatgtcagat ttgttatctt
     4021 ccatcgcctg tttctcggca tttttcttct gcatctccag ttcataaagc ttcgctttca
     4081 tctgcttcat ggcctgatct ttgttcttgt gctgggaacg gtcgttctgg cactgggtca
     4141 cgatcccggt cgggatgtgg gtaatacgca ccgcagattc ggtacggtta acgtgctgac
     4201 cgcccgcgcc ggacgcgcga taaacgtcaa tgcgcagatc cgccgggttg atttcgatat
     4261 caatatcatc atcaacttcc ggataaacaa acgcagagct gaacgacgtg tggcgacgac
     4321 cgccggagtc aaacgggctt ttacgcacca ggcggtgaac gcccgtttct gtacgcagcc
     4381 agccgtaagc gtaatcgccg gagattttga tcgtcacgga tttaataccc gccacttcac
     4441 cttccgactc ttcgatgatt tcagttttga aaccacgcga ttctgcccag cgcagataca
     4501 tacgctcaag catgctcgcc cagtcctgtg cttccgtacc gccagacccc gcctgaatat
     4561 cgaggtaaca gtcggcgctg tcatattcgc cagagaacat acggcggaac tcaagctgcg
     4621 ccagtttttc ttccagggcg tcgagttcag caacggcttc gttaaaggtt tcttcgtcgt
     4681 cagcttctac agccagttcc agcagaccag aaacatcttc cagcccctgt ttcatttggt
     4741 cgagggtgtc gacaacggct tcgagggagg aacgctcttt acccagcgcc tgtgcgcgtt
     4801 cgggttcgtt ccagacatcc ggctgttcca gctcggcgtt tacttcttcc agacgctctt
     4861 tcttggcgtc gtagtcaaag atacccccta agaacgtcgg agcgttccgt gaggtcctga
     4921 atgcgattat ttaccggatt aatttcaaac atggtctgat ttcttttatt gagctagtca
     4981 aaatgcggtg ataagagcgg gattgtaccc aatccacgct cttttttata gagaagatga
     5041 cgctaaattg gccagatatt gtcgatgata atttgcaggc tgcggttgcc gcgaaactcg
     5101 ttgatatcga gcttataagc cagttgcact tcgcggacgc cgttatccgg ccagagagcg
     5161 gtatcgacat taaaagcaat accatccagc agtggaccgc cgccgaccgg ttcgaccatc
     5221 accttcaaat gacgttcgcc caccagccgc tgttgcagca gacggaaatg accgtcaaac
     5281 agcggctccg ggaacatctg cccccacggg ccagcatcgc gcagcagctg cgccacttcc
     5341 atggtcattt cggccgggct taacggaccg tctgacacca cttcgccttg caatagcgaa
     5401 ggggccagcc actcagtaac cagttcgcca aaccgttgtt gaaagagttc gaatttatcc
     5461 tcttccagcg acaaacccgc cgccatcgca tgaccgccaa acttgagcat catgccgggg
     5521 tagagtgtgt ctaatcgctc cagcgcatca cgcatatgca gcccctgaat ggagcgaccg
     5581 gaacctttca gcgtaccgtc acctgctggc gcaaaggcga taaccggacg gtgaaaacgc
     5641 tctttgatac gcgaagccag aataccgaca acgccctgat gccattcggg gtgatacatt
     5701 gccagcccgc cgggtagcgt gtcacggctg cgctccagtt tctcgcacag ggtcagggct
     5761 tcaacctgca ttccttgttc gatctctttt cgcgtctggt ttagcgcatc gagttcattt
     5821 gccagcacgc gcgcttcgcc gatgttgtcg cacagcaaca gcgccacacc aacggacata
     5881 tcatccagtc gcccggcagc attgagacgc ggtcccagcg caaaacctaa atcgctggcg
     5941 gctagttttt gtgcatcgcg atttgccact tccagcagcg ctttaatccc tggacggcac
     6001 tttccggcac ggatgcgact catcccctgc caggtcagaa tgcgattatt agcgtccagc
     6061 ggcacgacgt ccgctactgt ccccagcgct accagatcca gcagttctgc caggttagga
     6121 attgcgatgc cgcgctcatc aaaccagccc tgatcgcgca aaaaggtgcg cagcgccagc
     6181 atcagataaa acgccacacc cacgcctgcc agtgatttcg acgggaaatt acagtcgcgc
     6241 aagttagggt taatgatcgc ttccgctgcg ggtaatgtgt cgcccggcaa atggtgatcg
     6301 gtaacaataa ccgggatgcc caacgagcga gcgtgctcaa ctcccgcatg ggaggaaata
     6361 ccgttatcca ccgtgacaat taactgcgcg ccacgggcat gggcctgatc gaccacttcc
     6421 gggcttaagc cgtaaccgtc ttcgaaacgg tttggtacca ggtagtcgat attgctgcaa
     6481 ccaagcgagc gcatcgccag cacgcttaga gccgtgctgg tcgcgccgtc ggcgtcgaaa
     6541 tcaccgacca caataatccg cgttccttcg cgaaaagcgt tgtaaaggat ctcaacggcc
     6601 ttttcgacac cgcttagttg ctgccagggc aacatacctt taacactgcg ttccagttct
     6661 tgcgcactgc gtactccccg gctggcgtat aaacggcgca gcaagggagg caattcagcg
     6721 ggcaagtctg ccgtttcatc gacttcacgg cgacgaagtt gtatctgttg tttcacgcga
     6781 attatttacc gctggtcatt ttttggtgtt cgtcgaggaa ttctttcatg tctttcggcg
     6841 gctggtaacc cggaacaagt gtgccattgc tcagcacaac tgccggagta ccgctaacgc
     6901 caagctggac gccaagtgcg taatggtcgg caatatccac gtcgcagctg gctggtgcga
     6961 cgcttttacc tgccatcaca tcatcaaacg ctttgttttt atctttcgca caccagatag
     7021 ctttcatttc tttctctgca tcgctgtcca gcccctggcg cgggaaagca agataacgca
     7081 cggtgatccc cagcgcgtta tagtctgcca tttgctcatg cagtttgtgg cagtaaccac
     7141 aggtaatatc agtaaacacg gtgatgacgt gtttttcctg cggcgcttta taaacgatca
     7201 tctctttttc aagcgcattc aactgcttta acagcatctt attggtgaca ttgaccggag
     7261 ccgtgccact aacgtcatac attggcccct gaatgatatg tttaccatca tcggtgatgt
     7321 acaacacacc gctgttagtc agaactgtct tcatgccagc tacgggcgcg ggctgaatat
     7381 cgctgctttt gatgcccatt ttggctaacg tttgttgaat tgccgcgtca tcagcctgag
     7441 caaagcctga aaacgccgct aacaaagtaa acaacataaa acctttcttc ataaatcttc
     7501 ccgttctttt cagacatcac gcccgcgggt gatgctgttg atgaagttgc cgcagacgct
     7561 cggtagcgac atgcgtataa atttgcgtgg tggaaagatc gctgtggccc agtagcatct
     7621 gcaccacgcg taaatccgca ccatgattta ataaatgagt ggcaaaagcg tgacgcaaca
     7681 catgcggtga cagcttttcg ctgtcgatac ccgccagcac ggcataatgt ttaatgcggt
     7741 gccagaaggt ctgtcgcgtc atctgctgcg cacgctggct gggaaacaac acgtcaattg
     7801 acacaccatt cagcagccac ggacgcccat gttccagata ggtttccagc cagtaaaccg
     7861 cctcttcccc taacggcacc aggcgctctt tgttgccttt accaatgacc cgcactacgc
     7921 cctgacgcag gctgatatca ctcattgtca gcccgaccag ttcagagaca cgcagcccgg
     7981 tagcatacaa cacttcaagc atggctttat cgcgtagctc cagtggctga tcaattaatg
     8041 gcgcctgtaa taaacgttcg acctgcgctt cgcttaaatc ttttggcaaa cgctggggca
     8101 atttcggtga agcgagatgc gcactgggat cgtcttcacg aaacttttcg cgataaagat
     8161 actggaacaa tcggcgcact gcactcagca aacgcgctga gctggtggct ttatacccgc
     8221 cctccagccg ttctgccagt aatgcctgca aatcgtcact ttgcgccgtc gccagcgtca
     8281 acccgcggtg atgcaaccac tccaccatca ttgacagatc gcgacggtaa gcgttcaacg
     8341 tattttcagc cagatttttt tccagccaca gagcatcaag aaactgctcg atgcgtgcca
     8401 gatcctgttt cacttgcgcc ccttatggtc actcatttga tccattatgc cttattgtgc
     8461 cgtgactaaa gcgattctga tacactagct gcaaaagcca cagcagaatc gagaagctta
     8521 cgttatgaat atgggtcttt tttacggttc cagcacctgt tacaccgaaa tggcggcaga
     8581 aaaaatccgc gatattatcg gcccagaact ggtgacctta cataacctca aggacgactc
     8641 cccgaaatta atggagcagt acgatgtgct cattctgggt atcccgacct gggattttgg
     8701 tgaaatccaa gaagactggg aagccgtctg ggatcagctc gacgacctga accttgaagg
     8761 taaaattatt gcgctgtatg ggcttggcga tcaactggga tacggcgagt ggttcctcga
     8821 tgcgctcggt atgctgcatg acaaactctc gaccaaaggc gtgaagttcg tcggctactg
     8881 gccaacggaa ggatatgaat ttaccagccc gaaaccggtg attgctgacg ggcaactgtt
     8941 cgtgggtctg gcgctggatg aaactaacca gtatgacctt agcgacgagc gtattcagag
     9001 ctggtgcgag caaatcctca acgaaatggc agagcattac gcctgatctc actgacggct
     9061 tagcgcatat gctttgccgt catctttatc tttgcgtctc ttgttgcaac aaaatccgcc
     9121 gtaaatcccg ccattcggct tcgtccatgc tgtcggctgc cagccataaa tgttgccgtt
     9181 taccgccatc agaacgtaaa cgcagcatca tgccgctctt aatcatccac ggtgctttga
     9241 cgatgctcca ctcctgccct tgccaacgca aacgcccgtc catcaacaag cgaatttccc
     9301 cctggcgagc attaatacac cgctggctgc gaacgcaatc aaataccacc agcgaaagta
     9361 acaccatcca taacggggtg taactgagcg gccagggcat gagtaaaata acagcggcaa
     9421 ccagcccatg aatcagcaag gaaagccact gtgcgcgcca ggagacgcgc aaatcagatt
     9481 gccacaggac cacgttcccg gttccgtgtc tggatgagtc ggaccattat ttccagttct
     9541 gcatcggccg gtttaccgtg attcatcagc cagttaaaca ggtccggatc gtcacattcc
     9601 agcagacgaa taaagatgcg tttttcgtca tcgcttaagc tgtcgtactc atgttcgaaa
     9661 aacggcatga ttgaaatatc gagttcgcgc ataccacggc ggcatgccca atgaatgcgg
     9721 gctttgttgt taatgtccat cttcttcctg tctcacgaaa atccagtacc cggctattgt
     9781 aacgtgtttt tcgacttctt ttacgggaat atcagtaaac accatcgcga tcgcgaaatt
     9841 aattcacaac aattcaaggg cttcattttt ttggcagtcg cctcgcagaa ggcacagatc
     9901 gcgtagtgaa agcacttgca ttgcctcata gctcttttac cattagtcat taatacgccg
     9961 ttaagcaact caggactcta ttatggcttt tacacctttt cctccacgtc agcctacggc
    10021 ttctgcccgt ttaccgctga cgctgatgac gcttgatgac tgggcgcttg ccaccattac
    10081 tggcgcggac agcgaaaaat atatgcaggg tcaggtgaca gcagatgtca gccagatggc
    10141 agaagatcag cacctgctcg ccgcccattg cgacgccaaa ggtaaaatgt ggagcaattt
    10201 acgtctgttc cgcgacggcg atggctttgc atggattgaa cggcgcagcg tgcgtgaacc
    10261 gcagctgact gaactgaaaa aatatgcggt attctctaaa gtgaccatcg cgccagacga
    10321 cgagcgtgtg ctgcttggtg ttgccggttt tcaggcgcgc gccgcgctgg caaatctctt
    10381 tagcgaactg ccttcgaaag aaaaacaggt agtcaaagaa ggcgcgacca ctttgctatg
    10441 gtttgaacac ccggcagaac gtttcctgat cgtaaccgat gaagctactg ctaatatgct
    10501 gaccgataaa ctgcgcggtg aagcggaact gaacaatagc caacagtggc ttgcattaaa
    10561 cattgaagct ggtttcccgg tgattgatgc cgccaacagc gggcagttta tcccacaggc
    10621 gaccaatctc caggcgctgg gcggtatcag ctttaagaaa ggctgttata ccggacaaga
    10681 gatggtggcg cgagcaaaat tccgtggtgc caacaaacgc gcgctctggt tgctggcagg
    10741 tagtgccagc cgactgccgg aagctggtga agacttagag ctgaaaatgg gcgagaactg
    10801 gcgtcgtacc ggtacggtgc tggctgcggt aaaactggaa gatggtcagg tcgtggtaca
    10861 ggtcgtcatg aataacgata tggaaccgga cagcatcttc cgcgtacgcg acgatgcgaa
    10921 tacactgcat atcgagccgc tgccgtattc gctcgaagag taaatctctg tatcgcatca
    10981 ggcatttatc gcctgatgcg acgctgacgc gtcttatcat gtctgggatt tgatgccttt
    11041 atcacaacag ggacgaggcc cgtagatcgg ataaggcgtt cacgccgcat ccggcggttg
    11101 cgctccgatg cctgatgcga cgctgacgcg tcttatcagg cctacaaagg catacccatt
    11161 acgcctgccc aatatacaaa tagatcgcca gaaagtggca cacactaccg ccgagcacga
    11221 agccgtgcca gatggcatgg ttgtatggaa tgcgtttgca gacgtagaaa atcaccccga
    11281 gcgaataaac cacgccgcct accgccagta aggtaacgct gcccgccgcg agcttaactg
    11341 ccatttcata aattaccacc agcgacagcc agcccatcgc cagataagtc accagagata
    11401 aaattttgaa tcggtgcgcg atggtcagtt tgaacagaat ccccagcaat gctaggctcc
    11461 agataacaat catcaacccg cgcgctaacg gagaatccag ccccaccagc aaaaacggcg
    11521 tgtaggttcc ggcaatcaac aggtaaatag cgcaatggtc aaatttcttc agccacattt
    11581 ttgcccgttg atgaggaatg gcgtgataga gcgtcgaagc gaggaacagc aggatcatac
    11641 tgccgccata gaggctgtaa ctggttatcg ctgtggcgct ggcattgaga tccaccgcct
    11701 gaaccagtag caacaccagc ccaacgatac caaacaccag cccaatgccg tgactgacgc
    11761 tgttggcaat ttcctctgcc agcgaatatc cctgcttaat gaggggcttc tgaaccataa
    11821 cttactccag agaaacgtac acgcacatgt atacctctcc agcgtaactg agaatagttc
    11881 cagtgaacac ctgttagcta aaataaattt tgatttgaga ctaatctcct aaaaatcatg
    11941 aaattaaatg cgaaatttca actaacaggc gtgagttcaa tttaaagaca tttaaattca
    12001 atcatataaa attgtgtctg accgggatag atgtcagcaa tgaccttttt cagttcaggc
    12061 agggtcatat tttcctgctc tgcatgtttt tccgtcagtg tatccagcgt tacggttgag
    12121 gttgcggtga cttcaatcgt gcaaaaataa ccgtcatctt caaaacgtcc gacacgaagc
    12181 acatcacccg ttttgaagtg cgattcagac tcgtcgcgga tggtgatggt tttacgccca
    12241 gccagaatgt catcctggaa acgttgaaaa aaagtgatgt cgtttggctg catggtacta
    12301 tttcctgtaa gaattgactg atctggagcc tatgatagtg aaaaaactca ccttaccgaa
    12361 agatttctta tggggtggcg ccgttgccgc tcatcaggtc gaaggcggct ggaacaaagg
    12421 cggcaaagga ccgagcattt gtgacgttct gaccggtggc gcacacggcg tgccgcgcga
    12481 aatcaccaaa gaagtcttgc caggaaaata ctatccaaac catgaagccg ttgattttta
    12541 tggtcactac aaggaagaca tcaagctatt tgccgaaatg ggcttcaaat gttttcggac
    12601 ttccatcgcc tggacgcgta tttttccaaa aggtgatgaa gctcagccaa acgaagaagg
    12661 gctgaagttt tacgactctc tgttcgatga actgctgaaa tacaacatcg aaccggtgat
    12721 aaccctctcc cactttgaaa tgccgctgca tctggtgcag caatacggta gctggaccaa
    12781 ccgtaaattc gttgatttct ttgtccgttt cgcggaagtg gtattcgaac gctacaagca
    12841 taaagtcaaa tactggatga ccttcaacga aataaacaac cagcgtaact ggcgtgcgcc
    12901 gctgtttggt tactgctgct ccggcgtggt gtataccgag catgacaacc cggaagagac
    12961 gatgtaccag gtgctgcatc accagtttgt cgccagcgcc ctggcagtga aagccgcgcg
    13021 tcgcattaac ccggagatga aagtcggctg tatgctggcg atggtgccgc tctatcccta
    13081 ctcatgtaac ccggacgatg tgatgttcgc tcaggagtcg atgcgcgaac gctacgtctt
    13141 taccgatgtg cagctatgcg gctattaccc gtcctatgta ttgaacgagt gggaacgtcg
    13201 cggatttaat atcaaaatgg aagacggcga tctcgatgta ctgcgcgaag gcacctgcga
    13261 ttatcttggt ttcagctatt acatgaccaa cgcagtgaag gccgaaggcg gcaccggcga
    13321 tgcgatctct ggttttgaag gcagcgtacc gaacccgtat gttaaagcgt ctgactgggg
    13381 ctgcagattg atccagtagg tctgcgctat gccctttgcg aactgtatga gcgttatcag
    13441 aggccgctgt ttattgtcga aaacggtttt ggcgcttacg acaaagtgga agaagatggc
    13501 agcatcaacg acgactaccg cattgactac ctgcgcgccc atattgaaga gatgaaaaaa
    13561 gcagtgactt acgatggtgt ggatctgatg ggctacacac cgtggggctg catcgactgc
    13621 gtgtcattca ctaccgggca gtacagcaaa cgctacggct ttatctatgt gaataaacat
    13681 gacgacggta ctggcgatat gtcgcgttca cgtaagaaga gctttaactg gtacaaagag
    13741 gtgattgcca gcaacggcga gaagctttaa gtcgatgaag taccggatgc aatacttgtt
    13801 gcatccggtc agacaacttc cctgttttat ttcccgcccg ccaaatcgat aaaacttccc
    13861 gtgacgtaag aggctttatc acttagtagc cagacaatgg cctgcgcgac ctcttctgcc
    13921 tgtccaccac gctgcatggg gatgttcgac ttaacgcgat cgacgcgtcc aggctcgccg
    13981 ccgctggcgt gcatttcggt ataaataaac cctggccgca cgcagttaac gcggatcccc
    14041 tgcgcggcga cttccagcga tcgtccggtg gttaacgtat caatcgcccc tttcgatgcc
    14101 gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg ccaccgaaga gacattgacg
    14161 atagcgccgc cactgccacc atttttaagc gccatgcgtt ttaccgcctc gcggcagcag
    14221 agaaaatatc ccgtcacgtt ggtggaaagt actcggttga ttcgctctgc ggtaaggttt
    14281 tcaacggtgc actgggtaaa caagatcccg gcgttattga ccagcgctgc tagcggttca
    14341 tcgtgctgat cgattgctgt aaacatcgca acgacctggt tttcgtcgct gatatccgcc
    14401 tggagcacga atgctttgcc accggcttgc attattaagt tcatcacttc ctgcgccgcg
    14461 tggaggtttt gctgataatt aaccgccacc gtataccctt cttgcgccaa cagtaatgca
    14521 gttgcccgcc cgatgccgcg actgccaccg gtcacaagtg ctatagccat ttgtttttcc
    14581 gagaaaaatt aattcgttga cgtatctttg agattgctaa caaactggct caatcttcaa
    14641 tgttggcacg cttttccccc tcgccctttc agggagatgg tcggggtgag ggtaaatatt
    14701 cgcgccagtg ccggcctgtt cccctcaccc taaccctctc cccaaagggg cgaggggact
    14761 gtctgagcac ttttgtactt tgtcatctga ctaaaaaggc gccgaagcgc ctttagaaaa
    14821 tactcgaatc agtgaattac tggtattcgc taatcggtac gcaggagcag aacaggttac
    14881 ggtcgccgta aacatcatcc agacgtttca ccgtcggcca gtatttgtct gccacgcctg
    14941 ccgggaatac cgcaacttca cggctgtacg gatgcgccca ctcggcgacc agttcgctct
    15001 gaatgtgcgg cgcgttcacc agcgggttat cttccagcgg ccagacaccg gctttcacct
    15061 ggtcaatttc tgcgcggata gccagcatcg cgtcgataaa gcgatccagt tccactttgc
    15121 tttcagattc agtcggttca accatcagcg tacccgccac cgggaacgac atcgtcggcg
    15181 cgtggaaacc gtagtcgatc aggcgcttgg caatatccag ctcgctgatg ccgatttctt
    15241 ctttcagcgg gcgaatatcg agaatacatt cgtgcgccac gcgaccgtcg cgaccggtat
    15301 acagcaccgg gaaggcatcc tgcaggcggc tggcaatata gttggcgttg aggattgcca
    15361 cctggcttgc ttttttcagc ccttctgcgc ccatcatgcg gatgtacatc cagctgattg
    15421 gcaggataga ggcgctaccg aacggtgccg cagaaactgc gccctgacgg gttaacatgc
    15481 cttcgatttg caccacgcta tgacccggta caaacggtgc cagatgcgct ttcacaccga
    15541 tcggtcccat acccggacca ccaccgccgt gcggaatgca gaaagtttta tgcaggttga
    15601 ggtgcgaaac gtccgcgcca ataaagcccg gcgaggtgat gccaacctgg gcgttcatgt
    15661 tcgcgccatc aaggtaaacc tgaccgccga actgatgcac gacttcacac acttcacgga
    15721 tcgtttcttc atacacgccg tgggtagaag gataagtcac catgatacag gagaggttat
    15781 cgcccgcctg ttccgctttc gcgcgcagat cagtcagatc gatgttgccg tttttatcac
    15841 acgccacaac caccacctgc attcctgcca tatgtgcaga agcggggtta gttccgtgcg
    15901 cagaagccgg gatcaggcag atatcgcgat gcccttcgtt gcggctttca tgataatgac
    15961 gaatcgccag caggcccgcg tattcgccct gtgcgccgga gttcggctgc atacaaacgg
    16021 cgtcgtaacc ggtcagtttc accagccagt cagccagctg cgcaatcatc tgctgataac
    16081 cttcagcctg ttccggcggg caaaacgggt gcagttcggc aaattccgtc caagtgattg
    16141 ggatcatctc ggcggcggcg ttcagtttca tggtgcagga acccagcggg atcatcgcct
    16201 gattcagcgc cagatcttta cgctccagcg agtgcatata gcgcatcatt tcggtttcgc
    16261 tgtgatagcg attaaacacc ggatgggtga ggatttcgtc gtcgcgcagc atcgcaggct
    16321 ggatagagcg gctgtcgtga gccacgtctt tgtccagcgt gtcgatctcc agcccgtggt
    16381 tatcgcccag cagcacactg aaaagctgca ttacgttttc acgcgtggtg gtttcatcaa
    16441 gggtgatccc cacggcgttc aggatatcgc tacgcaggtt gatttcagcc gcttcggcac
    16501 gcgtcagtac gcccgctttg tcggctactt ccacacacaa ggtgtcgaaa tagtgcgcat
    16561 ggcgcagttt cagacctttt tgttgcaggc ccgccgccag aatatcggtc agacggtgaa
    16621 tgcggttagc gatacgtttc aggccaaccg ggccgtgata aacggcatac aggctggcga
    16681 tgtttgccag cagtacctgg gaagtacaaa tgttggagtt cgctttctca cgacgaatat
    16741 gttgctcgcg agtctgcatc gccatgcgca gcgcggtatt gccagctgca tctttcgata
    16801 caccgataat acggcccggc attgagcgtt tgtattcatc tttcgccgca aagaatgccg
    16861 cgtgtgggcc accgtagccc atcggcacgc cgaagcgttg cgccgaacca aaaacaatat
    16921 ccgcgccctg tttacccggc gcagttaaca gcaccagcgc cataatatcg gcggcaacgc
    16981 tgaccacaat tttgcgtgat ttcagttcgc taataagcgc agtgtagtcg tgaatttcac
    17041 cggtagtgcc tacctgctgt aacagcacgc cgaagacgtc ctgatggtcg agcacttttt
    17101 gcgcgtcatc gacaatcact tcaaaaccaa aggtttcggc acgagtacgg accacatcca
    17161 gtgtttgcgg atgcacattg gaagccacga agaagcggtt ggcatttttc agtttgctga
    17221 cgcgtttcgc catcgccatt gcttcggcgg cagcggtggc ctcgtccaga agagaagcag
    17281 aggccatatc cagtccagtc aaatccagcg ttacctgctg gaagttgagc agtgcttcaa
    17341 ggcggccctg ggagacttca ggttgatacg gagtgtacgc ggtataccag cccggatttt
    17401 ccagcatgtt acgcaggata accggcggta gctgcacggc ggtgtaaccc atgccgatgt
    17461 aagacgtgaa gcgtttattg cgactggcaa tagccttgag ttctgccagt gcggcgtatt
    17521 cggtcgccgg tgcgccaacc tgcggtggtg tcgcaagctg aatatctttc ggcacaatct
    17581 ggccggtcag cgcgtttaac gattgtgcac caacggcatt cagcatttct tgctgttgcg
    17641 cggcgtccgg tccgatatgg cgttcaataa aagcgccgct gttttcaagc tggcttaacg
    17701 tctgtgtcat gagcgatggt tcctgaaacg tgcagtgaat tgtgaacctc tctccttacg
    17761 aagagagtga gggtgaggca taaattttcc tcaccctgat cctctcccgc agaagaggaa
    17821 taaagccgtt actcatcttc taacaatgct tcgtatgcgg tcgcatccag cagtgattcc
    17881 agttcgcttt catcgctagc tttgatttta aagatccagc cgcctgcata cggttcgctg
    17941 ttcaccagtt ccggggaatc gctcagtgcg tcgtttaccg ccacgatttc accgcttact
    18001 ggcgcataaa tgtctgacgc cgcttttacc gattcggcaa ccgcgcagtc atcgcccgcg
    18061 ctaaccgttg cgcccacttc cggcaggtca acaaacacca tatcgcctaa cagctcctga
    18121 gcatgttcgg taataccaac ggtgtaagtg ccgtcggctt ctttacgcag ccattcgtgt
    18181 tctttgctgt atttcagttc tgctggtacg ttgctcatca atcaatctcc aaaaaagtaa
    18241 atcacgcgac ggctttgccg ttacgcacaa aaacaggttt tgtcacttta accggcattt
    18301 cacggttgcg aatttgcaca atcgccgttt cgccaatacc ttccggcacg cgcgccagcg
    18361 caatgctgta acccagcgtc ggggagaaag taccgctggt gataatgcct tcatgctggt
    18421 tgccctgcgc atcggtaaag cgtaccggca gttcattacg cagcacgcct ttttcggtca
    18481 tcaccagacc aaccagtttt tctgtaccat gctcacgctg cacttccagg gcttcacgac
    18541 cgataaagtc acgatctgtc ggttcccagg cgatagtcca gcccatgttg gcggctaaag
    18601 gagaaatagt ttcgtccatc tcctgaccat aaagattcat gcccgcttcc agacgcagcg
    18661 tgtcacgcgc ccccagaccg cagggcttaa cgcccgcttc caccagcgca cgccagaaat
    18721 cggccgcttt ttcattgggc agcgcaattt catagcccgc ttcaccggta taaccagtgg
    18781 tggcaataaa cagatcgccc acctgcacgc caaagaacgg tttcatccct tccaccgcct
    18841 gacgctgagc atcattaaac agtgtggcag cttttgcctg agcattcggc ccttgcacgg
    18901 caatcatgga aaggtcatca cgaacggtaa tttcgatgcc gaaaggttca gcgtgttggg
    18961 taatccagga gaggtctttt tcgcgggtgg cggagttaac aatgaggcgg aagaaatctt
    19021 cagtaaagta gtagacgatg agatcatcta tcacaccgcc agaggcattc aacatccccg
    19081 agtaaagggc tttgccgctt ttggtgagct tcgccacatc gttcgccagc agataacgca
    19141 gaaactcccg ggtgcggctg ccgcggagat cgacgatggt catatgcgac acatcaaaca
    19201 ttccggcatc ggtacgtacc gcatgatgtt cgtcgatttg cgaaccgtaa tgcagcggca
    19261 tcatccagcc gtggaaatcc accatgcgag cgccgcaaag cgtgtgttgt tcgtacaaag
    19321 gagtctgttg tgccatcttg tcctcattga ataagcgggg ctgacaactt tttcatggtg
    19381 aaattatcac cacaaaaccc agcatcggag ccactcccgg tccccaacgc aatcgttctc
    19441 ttttgcctga acttaccacc gaaacagact gttaaccata aggtaaaatt gatcatcaca
    19501 ttagcttatg gttaaaaaat gcaaaaatcg cgacagaata aaaaaccaaa aaatacacca
    19561 atttctatac aaagatgatg tgatgagaaa gtcaatttga ataagacaat attaagagct
    19621 aaaaaaatgt caaaaaacac taaatcaaaa aatcatggca ttagaaaata taatgcgaaa
    19681 acggaggtga aattagttta tttcaaatga ggaaaatctc ccggcgaaaa aaccgggaga
    19741 tgaaagtgtg atgggtatca aataaacaac agaggagaaa tttttaacgc agccattcag
    19801 acaaatcgtt taatcccatt gcctggcgga taagttgcgg cttaacgcca ggaagcgtgt
    19861 cggccagttt caaaccaata tcacgcagca gttttttcgc cggattagcg ccggaaaaga
    19921 gatcgcgaaa accctgcata ccagccagca tcagcgccgc gctgtgcttg cggctacgtt
    19981 catagcggcg caggtaaatg tactgcccga tgtctttacc ctgacgatgc aaccgtttca
    20041 gttcagcaat cagctctgcg gcgtccataa atccgagatt aacgccctgc cccgccagtg
    20101 ggtgaatagt atgcgcggcg tcgcccacca gcgccagacg gtgtgcggca aactggcgcg
    20161 cataacgccc cgtcagcggg aacacctgac gcgcgctctc aaccttgcat aagccaaggc
    20221 gattatcaaa agcgatattt aacgcgcgat taaattcgtc ttcacttgcc tgctgcatcc
    20281 gctgcgcttc ctctggcgac agtgaccaga caatcgagca aagatgcgga tcgctaagcg
    20341 gtaaaaaggc cagaatgcct tcgccatgga aaacctgccg cgccaccgca tcatgcggtt
    20401 cttccgtgcg aatggtcgct accagcgcgt gatgctgata atcccagaaa gtcagcggaa
    20461 tatctgcttt gttgcgcaac caggaattag caccgtccgc gccaatcacc agacgcgccg
    20521 ttaacatgct gccatctttc agcgtcagga aggtttcatt ttctccccag gcgacctgct
    20581 gtaattctgt gggggccaac agagtaatat ctgacgactg ctgcgctttg ttccacagcg
    20641 cgtagtgaat cactgagttt tcaacgatgt gcccaagatg gctatagccc atgctttgat
    20701 cgtcaaacga gatgtgacca aaactgtctt tgtcccacac ttccatgccg tgatagcaac
    20761 tggccctacg agcgagaatg tcctgccaga cgccaagacg ggtgagtaat ttttcgctgg
    20821 cggcattgat agccgaaacg cgcagttgtg gtagtgcatc taccgccaga ggttccggta
    20881 cgcgctgctc cagtacggca acgcgtaagc cgctcccctg taagccgcag gcaaccgcca
    20941 gccccaccat gccgccgcca acaatggcta catcaacact ttgcattgtt tattccttaa
    21001 aaccgccttc aacgcgccac ccaaccgagg gtgcgctgcg ccagcacatc gcgtgccggg
    21061 gtgaataatt ccatcgtcat cagtccgatg ttgcgcccga caaccagcgg tgcccaacgg
    21121 ttggcaaaaa gatgtaccag gctgtccgtg acgccgatgg ttgcttcgcg atcgctctgt
    21181 cgacgctgct gatagcggca caatatgccg taatcaccga tgtcttctcc gcgctcatgc
    21241 gcctgagtca gggtttccgc aaggctcatg acatctcgca taccgaggtt aaacccttgc
    21301 ccggcaatcg ggtgcagagt ttgcgccgca ttgcccacca gcacagtgcg atgggtaatc
    21361 ggtctggcgg cgcgggttaa tgccagcgga taagcactgc gtttaccggc gtgggtaatt
    21421 tgccccagtc gccagccaaa ggcagactgg agttcacggc aaaacttctc gtcgctccac
    21481 gacagcacct cttcgcgccg ttccagtgga tgacaccaga ccagcgaaca gcgtccgtca
    21541 gacatcggca acatcgccag cgggccatgt tgcgtgaagc gttcaaaagc gcggccttcg
    21601 tgcgcaacag aagtagcaac gttggcaatc actgccagtt gttcgtaagg ctcctgctgc
    21661 caatcaacgc cgcacgcggt ggctaacgct gaatgggtgc catcagcggc tatcagcacg
    21721 cggcccgtca gcatctcgcc gctctccagc gtcacttcaa cgtgactctg agtacgggca
    21781 acgttagcta cgcgatcggg gcaatgcagc attacgccag gtgcttttcg cagcaatgca
    21841 aacagccgtt gcccaacatt atgcaattcg acaacctgcc ccagcgccgc cagttggtaa
    21901 tcttctgcgg cgagggtgac gaatccagca tgaccacgat cgctgacatg cacggtagtg
    21961 atggcggttg cgcaatcagc cagagattgc cagacgccga tgcgcgccag ttgctgacag
    22021 gtacccgccg ccagcgctat cgctcgtcca tcaaagcccg gatgagcatg tgactctggc
    22081 gcagtcgctt caatcaaatg taccggcagc gccccgtgac ttaaccggga aatagccagc
    22141 gccagcgtcg cgcccgccat gccgccaccg acgatgatta cgctcattgc tttctcgcag
    22201 cagccatcaa cgcttcgatt tcttccggct ttttcaccac gctggcggtg aggttttcgt
    22261 tgccggtttc ggtaatcaca atgtcgtctt caatacgaat gccgataccg cgatattgtt
    22321 ctggcacatc cgcatccggc gcgatataca gaccaggctc tacggtcagt accatccccg
    22381 gttccagaat gcgcgagcga tcctgaccat aaacgccaac gtcatggaca tccagtccta
    22441 accagtggct aaggccatgc ataaagaaag gacgatgggc gttctgagcg atcagttcat
    22501 caacattacc tttcaggatg ccgagtttta ccaggccgct aaccatgatg cgcaccactt
    22561 caccagtgac ttccagaatg gaagttcccg gacgatacag gcgcaggctg gtttcgagag
    22621 actccagcac aatgtcgtag atttcacgct gggcctgggt gaatttgccg ttgaccggga
    22681 aggtgcgggt aatatcgccc gcgtaacctt tgtattcaca acccgcgtca atcaacacca
    22741 ggtcaccgtc gcgcagttca ctctcgtttt cggtgtagtg cagaatgcag ccgttttcac
    22801 cgctgcctac aatggtgtta taggaaggat agcgcgcacc gtggcggtta aattcgtggt
    22861 gaatttcgcc ttccagatgg tactcgaaca ttcccggacg gcatttttcc atcgcccgtg
    22921 tatgtgccat ggcggtgatt tctcccgcgc ggcggagtac ggcaatctct tctggcgatt
    22981 tgaacaggcg catttcatga acaacaggac gccagtcgat catcgttgcc ggtgcggtga
    23041 gattttgccg cgaaccttta cgcagttttt ccagcgcact gttcacgatt tcatcagcat
    23101 atgcatattc gccctgggca tggtaaacca catccaggcc gttaagtagt tgataaagtt
    23161 gctgattgat ttcgctgaat gccagtgcgc ggtcaacgcc cagtttctct ggcgcggcat
    23221 cctggcctaa gcgacggcca aaccagatct ccgccgtcag gtcgcgaacg cggttaaaca
    23281 gaacgctgtg gttatgagtg tcatcgcttt taatcagcac cagcaccgct tccggttcgt
    23341 taaagccggt gaagtaccag aagtcactgt tctgacgata ggggtattcg ctgtcggcgc
    23401 tacgtgttac ttctggtgca gcaaaaatca gcgcggcgct gccgggttgc atttgctcca
    23461 ccagggcctg acggcgacgc tgaaactctt gccgggatat ctcactcata acactctcct
    23521 tacgtttttt gtttttagtg tagagtcggt ttttgtactt ctggcgcggt cggttgcgga
    23581 tgagtaaagg tgtcgtggca taacagcgcg gcaacacgaa cgtattcgat gatctcttca
    23641 agcgacattt caagctcttc ctgatcttcg tcttcgtcgt agcccagctg cgcgatgtta
    23701 cgcagatcgt cgatggcttc accggtttcg ccggtcactt tgtccagctt cggttgcgta
    23761 acgccaagac caagcaggaa gtgattgacc caacctgcca gtgcatcagc ccgatcgaaa
    23821 acgctgacat catcgccatc aggcagataa agctgaaaaa ggaagccgtc atcctgcagg
    23881 gcatcgctgg tggcagagtg cattttacgc agtgcctgtg ccagctcatg accgaaagcc
    23941 atgccttcgt tcgtcaggtc gtgaagtagc ggcagccatg agctgtcatc gttaccgcca
    24001 catatcatcc cactgattaa accatgcatc tcggcagggg tcagacccgt cccttgttgg
    24061 ttcagatact ggttcatttc gttgtaacca ggcatttcgt tctgtataga cataagcatt
    24121 cgtcgtcaaa gggaggaata ttcatgatat gctaccactt tgggccctgg tggaccagaa
    24181 aagggcttgt ctcttctcat cagggtagct atagcgtcgc cccttcgcag accatgggtc
    24241 taaagacgaa ggcagcgcag tcaatcagca ggaaggtggc atgtctgcac aacccgtcga
    24301 tatccaaatt tttggccgtt cactgcgtgt gaactgcccg cctgaccaaa gggatgcgtt
    24361 gaatcaggca gcggacgatc tgaaccaacg gttgcaagat ctgaaagaac gcactagagt
    24421 cacaaatact gaacagttgg tcttcattgc cgcattgaat atcagctatg agttagcgca
    24481 agaaaaagca aagactcgtg actacgcggc aagtatggaa cagcgtattc ggatgctgca
    24541 gcagaccata gaacaagcgt tacttgaaca aggtcgcatc accgaaaaaa ctaaccaaaa
    24601 ctttgaatga cacttttcgg tttactgtgg tagagtaacc gtgaagacaa aatttctctg
    24661 agatgttcgc aagcgggcca gtcccctgag ccgatatttc ataccacaag aatgtggcgc
    24721 tccgcggttg gtgagcatgc tcggttcgtc cgagaagcct taaaactgcg acgacacatt
    24781 caccttgaac caagggttca agggttacag cctgcggcgg catctcggag attcccttct
    24841 tatctggcac cagccatgac gcaactacca gagctcccac tgacattatc ccgacaagaa
    24901 atccgcaaaa tgattcggca acgtcgtcgt gcgttaacgc cggaacaaca gctggaaatg
    24961 ggtcaacaag ccgctacccg gatgatgact tatcccccgg tggtgatggc gcatacggtc
    25021 gctgtattcc tctcttttga tggtgaactc gacacccagc cgctaataga acaactctgg
    25081 cgcgccggta agcgcgtata tcttccagtt ttgcatccct ttagtgccgg taatttgctg
    25141 ttcctgaatt accatccgca aagcgaactg gtgatgaaca ggttgaagat ccatgagcca
    25201 aaactggatg tgcgtgacgt gctacccctt tcccgattag acgtgctgat cacaccgctg
    25261 gtcgcctttg atgagtacgg tcagcgcctg ggaatgggcg gtggttttta tgatcggacc
    25321 ttacaaaact ggcagcacta taaaacgcaa ccggtgggtt atgcgcatga ttgtcagttg
    25381 gtggaaaaac tccccgttga agagtgggat atccctcttc ctgcggtggt tacaccgtcg
    25441 aaagtctggg agtggtaagg gcgatacacc tgcatcgccc tgattgacat cgttgattct
    25501 ttaacctaat ttagtgagta agggtaaggg aggattgctc ctcccctgtg actgttaata
    25561 agctgggaaa cttatgagta acagtacaat cagtatgatg gcaagtcgca tcataaccct
    25621 tctccttcaa gccctcgctt cggtgagggc tttaccgtta cagccccatg ctgcccagct
    25681 atcgtaagct ctctttaaat aaccacaacg cattgatcta cctttaaatc ttttcttcga
    25741 gcgtgctcga aaaaccccat gtaaaacaga gataattttt gggaaaaagg atctattacg
    25801 gcagtaaacg tgggcgatga agtggctatc aaaaagttcg gttcagggcg atgcaccccg
    25861 catcgccctg attgacatcg ttgattcttt gacctaattt agtgagtaag ggtaagggag
    25921 gatgactcct cccttgagac tgactgttaa taagctccga aacttatcag taacagtaca
    25981 atcagtatga tgacaagtcg catcataacc cttctccttt aagccctcgc ttcggtgagg
    26041 gctttaccgt tacagcccca tgctgtcccg ccatcgtaaa tccccattaa ataaacacaa
    26101 cgcattgatc tgactttgat ttattttctg gagcagactc gcaaagtaga atgcgcaacg
    26161 cggcaacggt gtggagaagg gataaaaaaa cgggcaagtc agtgacctgc ccgttaattt
    26221 tcagagaagg ggaattagta cagcagacgg gcgcgaatgg tacccggaat agctttcatt
    26281 gcctgcagcg ctttttcggc aacgtcttcg tcagcttcaa tatcaataac tacataaccc
    26341 atctgggcgg aagtttgtag atattgcgcg gcgatgttga cgccctgctc ggcgaagatt
    26401 ttgttcagcg cagttagcac gcccggacgg ttttcgtgga tgtgcatcag acgacgacca
    26461 ccatgcagtg gcagcgagac ttccgggaag ttcaccgcag agagcgttga gccattgtca
    26521 gaatacttga tcaatttacc cgcaacttcc aagccgatat tctcctgcgc ttcctgagtc
    26581 gaaccgccaa tgtgtggcgt cagaaggacg ttgtcgaatt cacacagtgg agaggtaaat
    26641 ggatcgctat tggtcgccgg ttccgtcggg aatacgtcga ttgccgcccc cgccagatgt
    26701 ttgctcgcca gcgcatcaca cagcgccgga atatccacca cagtaccgcg cgaggcatta
    26761 atcagcagcg agccgggctt cattagcgaa atctctttcg cgcccatcat atttttggtg
    26821 gacggattct ctggtacatg cagactcacc acatcgctca tattcagcag gtcagaaaga
    26881 tgctgtacct gagtggcgtt gcccagcggc agtttgtttt caatatcata aaagtaaaca
    26941 tacattccca gcgattcagc cagaatgccc aattgcgtac caatatgacc gtagccgatg
    27001 atacccagct ttttgccgcg cgcttcaaaa gaacccgccg ccagtttgtt ccacacgcca
    27061 cggtgcgctt tagcattggc ttccggcacg ccgcgcaata gcagcagcag ttcgccaatc
    27121 accagctccg caacagagcg cgtatttgag aacggtgcgt taaataccgg gatcccgcgc
    27181 tttgccgccg catccagatc aacctggttt gttccgatac agaaacagcc aatagcgacc
    27241 agtttttctg cggcgttgat cacgtcttca gtcagatggg tacgggatcg caggccgatg
    27301 aagtgggcat cgcggatgga ttcttttaat tgttcatcat ccagcgcgcc tttgtgaaat
    27361 tcgatgttgg tgtaaccagc tgcacgaagg ctttccagcg ccttttggtg cacgccttct
    27421 accagcagaa acttaatctt gtctttctcc agcgatacct ttgccattta cccaatcctg
    27481 tcttttgaaa tgttgtgtgc ggatttgcat ccgcctttca acatatcaaa aaataatatt
    27541 gcggcaatat gaacgtttgc gtcgcgatgt tgaggaaata tcacgcaaag gtaattcttg
    27601 gatgaatatg ctggtttaga ggatttattt agagcaatcg acaattgcct ggtaaaagcg
    27661 tgacacatgt caccaaattt aatgaagaga atttttttaa cgggggaggt tcccccgtca
    27721 gatcatttca caatggtttt gacaccgtca ggtgtgccaa tcagcgcaac gtccgcgcca
    27781 cggttagcaa acaagccaac agtcaccacg ccaggaatcg catttatggc gttttccatc
    27841 gctatcgggt caaggatttc catgccgtgg acgtcgagga tcacgttgcc attatcggtc
    27901 accacgccct gacggtattc cggacgaccg cccagtttca ccagctggcg cgccactgca
    27961 ctacgtgcca tcgggataac ttctactggc agcgggaatt tacccagaat atcaacctgc
    28021 ttggaggcgt cggcaataca gataaatttt tccgcaaccg aagcaatgat tttttcacgg
    28081 gtcagcgccg cgccgccgcc tttgatcatt tgcatgtggc cgttgatttc atctgcgcca
    28141 tcaacgtaga tgccaaggct gtcgacttcg ttgagatcaa aaacgtgaat gccgaggctt
    28201 ttcagttttt cagtggaagc atctgaactg gaaacggccc cttcaatctg gcctttcatc
    28261 gtgccgagcg cgtcaataaa gtgtgcggcg gtggaacctg tacctacacc aacaatggta
    28321 ccgggctgaa catactgaag tgccgcccat cctactgctt ttttcaattc atcctgcgtc
    28381 atgatcgttt cgcctgtggt atgaaatttc acacgcatta tatacaaaaa aagcgattca
    28441 gaccccgttg gcaagccggg tggttaactc atccataaaa tatcgcgcaa tggcgggcat
    28501 cccctttcgc cccgcaaata aagcatacaa cggtctgggt atgccgctcc acggtgcgaa
    28561 cagaggcaat aacgccacgc cattcaacat aaccagggcg cgaagtcgct aagcgaaatg
    28621 caatagcgca aatccctgat cttttaagct gcgcgtttaa ccattgatac aaatgttacc
    28681 cgccgacggc ttcggtatat gcaacctgac acaaaattgt gtcatagtgc aggaaaaagc
    28741 atttaccagg agcagacaac agcaatgaaa cgcccggact acagaacatt acaggcactg
    28801 gatgcggtga tacgtgaacg aggatttgag cgcgcggcac aaaagctgtg cattacacaa
    28861 tcagccgtct cacagcgcat taagcaactg gaaaatatgt tcgggcagcc gctgttggtg
    28921 cgtaccgtac cgccgcgccc gacggaacaa gggcaaaaac tgctggcact gctgcgccag
    28981 gtggagttgc tggaagaaga gtggctgggc gatgaacaaa ccggttcgac tccgctgctg
    29041 ctttcactgg cggtcaacgc cgacagtctg gcgacgtggt tgcttccggc actggctcct
    29101 gtgttggctg attcgcctat ccgcctcaac ttgcaggtag aagatgaaac ccgtactcag
    29161 gaacgtctgc gccgcggcga agtggtcggc gcggtgagta ttcaacatca ggcgctgccg
    29221 agttgtcttg tcgataaact cggtgcgctc gactatctgt tcgtcagctc aaaaccgttt
    29281 gccgaaaaat attttcctaa cggcgtaacg cgttcggcat tattgaaagc gccagtggtc
    29341 gcgtttgacc atcttgacga tatgcaccag gcctttttgc agcaaaactt cgatcttcct
    29401 cctggcagcg taccctgcca tatcgttaat tcttcagaag cgttcgtaca acttgctcgc
    29461 cagggcacca cctgctgtat gatcccgcac ctgcaaatcg agaaagagct ggccagcggt
    29521 gaactgattg acttaacgcc tgggctattt caacgacgga tgctctactg gcaccgcttt
    29581 gctcctgaaa gccgcatgat gcgtaaagtc actgatgcgt tactcgatta tggtcacaaa
    29641 gtccttcgtc aggattaatc catcaaataa tgcctgatag cacatatcag gcgttgtcct
    29701 cacttctttt tgtattcctt gaatcacatc acaaaataga caaa
//
LOCUS       DAJTYI010000038        28831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYI010000038 DAJTYI010000000
VERSION     DAJTYI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..827)
                     /gene="araF"
                     /locus_tag="OO564_001649"
     CDS             complement(<1..827)
                     /gene="araF"
                     /locus_tag="OO564_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6652808.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     "
     gene            1624..2127
                     /locus_tag="OO564_001650"
     CDS             1624..2127
                     /locus_tag="OO564_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6652809.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2207..2458)
                     /locus_tag="OO564_001651"
     CDS             complement(2207..2458)
                     /locus_tag="OO564_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6652810.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2573..2659)
                     /gene="azuC"
                     /locus_tag="OO564_001652"
     CDS             complement(2573..2659)
                     /gene="azuC"
                     /locus_tag="OO564_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6652811.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            2922..3245
                     /locus_tag="OO564_001653"
     CDS             2922..3245
                     /locus_tag="OO564_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6652812.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3416..3913
                     /gene="ftnA"
                     /locus_tag="OO564_001654"
     CDS             3416..3913
                     /gene="ftnA"
                     /locus_tag="OO564_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6652813.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(3950..4189)
                     /locus_tag="OO564_001655"
     CDS             complement(3950..4189)
                     /locus_tag="OO564_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6652814.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4379..5590
                     /gene="tyrP"
                     /locus_tag="OO564_001656"
     CDS             4379..5590
                     /gene="tyrP"
                     /locus_tag="OO564_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6652815.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5652..6317)
                     /gene="yecA"
                     /locus_tag="OO564_001657"
     CDS             complement(5652..6317)
                     /gene="yecA"
                     /locus_tag="OO564_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6652816.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6513..6599)
                     /locus_tag="OO564_001658"
     tRNA            complement(6513..6599)
                     /locus_tag="OO564_001658"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6563..6565),aa:Leu,seq:taa)
     gene            complement(6612..6685)
                     /locus_tag="OO564_001659"
     tRNA            complement(6612..6685)
                     /locus_tag="OO564_001659"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6651..6653),aa:Cys,seq:gca)
     gene            complement(6967..7515)
                     /gene="pgsA"
                     /locus_tag="OO564_001660"
     CDS             complement(6967..7515)
                     /gene="pgsA"
                     /locus_tag="OO564_001660"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6652817.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7572..9404)
                     /gene="uvrC"
                     /locus_tag="OO564_001661"
     CDS             complement(7572..9404)
                     /gene="uvrC"
                     /locus_tag="OO564_001661"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6652818.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9401..10057)
                     /gene="uvrY"
                     /locus_tag="OO564_001662"
     CDS             complement(9401..10057)
                     /gene="uvrY"
                     /locus_tag="OO564_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6652819.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10353..10529
                     /locus_tag="OO564_001663"
     CDS             10353..10529
                     /locus_tag="OO564_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652820.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10516..10740
                     /locus_tag="OO564_001664"
     CDS             10516..10740
                     /locus_tag="OO564_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6652821.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(10773..11393)
                     /gene="sdiA"
                     /locus_tag="OO564_001665"
                     /pseudo
     CDS             complement(10773..11393)
                     /gene="sdiA"
                     /locus_tag="OO564_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11623..12375)
                     /gene="yecC"
                     /locus_tag="OO564_001666"
     CDS             complement(11623..12375)
                     /gene="yecC"
                     /locus_tag="OO564_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6652822.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12372..13040)
                     /gene="tcyL"
                     /locus_tag="OO564_001667"
     CDS             complement(12372..13040)
                     /gene="tcyL"
                     /locus_tag="OO564_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6652823.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13055..14041)
                     /gene="dcyD"
                     /locus_tag="OO564_001668"
     CDS             complement(13055..14041)
                     /gene="dcyD"
                     /locus_tag="OO564_001668"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6652824.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14146..14946)
                     /gene="tcyJ"
                     /locus_tag="OO564_001669"
     CDS             complement(14146..14946)
                     /gene="tcyJ"
                     /locus_tag="OO564_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6652825.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15034..15585)
                     /gene="fliZ"
                     /locus_tag="OO564_001670"
     CDS             complement(15034..15585)
                     /gene="fliZ"
                     /locus_tag="OO564_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6652826.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15631..16350)
                     /gene="fliA"
                     /locus_tag="OO564_001671"
     CDS             complement(15631..16350)
                     /gene="fliA"
                     /locus_tag="OO564_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6652827.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16515..18167)
                     /gene="fliC"
                     /locus_tag="OO564_001672"
     CDS             complement(16515..18167)
                     /gene="fliC"
                     /locus_tag="OO564_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6652828.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18433..19839
                     /gene="fliD"
                     /locus_tag="OO564_001673"
     CDS             18433..19839
                     /gene="fliD"
                     /locus_tag="OO564_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6652829.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            19864..20274
                     /gene="fliS"
                     /locus_tag="OO564_001674"
     CDS             19864..20274
                     /gene="fliS"
                     /locus_tag="OO564_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6652830.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20274..20639
                     /gene="fliT"
                     /locus_tag="OO564_001675"
     CDS             20274..20639
                     /gene="fliT"
                     /locus_tag="OO564_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6652831.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            20717..22204
                     /gene="amyA"
                     /locus_tag="OO564_001676"
     CDS             20717..22204
                     /gene="amyA"
                     /locus_tag="OO564_001676"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6652832.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22238..22651)
                     /locus_tag="OO564_001677"
     CDS             complement(22238..22651)
                     /locus_tag="OO564_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6652833.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            22838..24043
                     /gene="yedE"
                     /locus_tag="OO564_001678"
     CDS             22838..24043
                     /gene="yedE"
                     /locus_tag="OO564_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6652834.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24040..24273
                     /gene="yedF"
                     /locus_tag="OO564_001679"
     CDS             24040..24273
                     /gene="yedF"
                     /locus_tag="OO564_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6652835.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24380..25050
                     /locus_tag="OO564_001680"
                     /pseudo
     CDS             24380..25050
                     /locus_tag="OO564_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25090..26280)
                     /locus_tag="OO564_001681"
                     /pseudo
     CDS             complement(25090..26280)
                     /locus_tag="OO564_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            26348..26963
                     /gene="tnpA"
                     /locus_tag="OO564_001682"
                     /pseudo
     CDS             26348..26963
                     /gene="tnpA"
                     /locus_tag="OO564_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27291..28373
                     /gene="ompC"
                     /locus_tag="OO564_001683"
     CDS             27291..28373
                     /gene="ompC"
                     /locus_tag="OO564_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6652836.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT         7280 a         7264 c         6764 g         7523 t
ORIGIN      
        1 agcagggaac cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca
       61 ccgttaatac caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt
      121 acgccgccaa gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga
      181 tgttgaacca gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta
      241 ggtacctgat aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc
      301 gttgtacggc ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct
      361 ttgacatccc agccacgttt ctgcatctct ttatacagtt cctggccctg acgttcgcca
      421 attttagtcg ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta
      481 acaaactggt catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg
      541 gcagagccga gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc
      601 aggctgtcga tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca
      661 aaccctaaat ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct
      721 tccggttgct tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata
      781 acggctgcca gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc
      841 tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca
      901 aagcattacc ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt
      961 aattctccat aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt
     1021 agcgggaaaa gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga
     1081 ttaatccata agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt
     1141 ctttttaacc agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa
     1201 ccacaactca aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc
     1261 tcattaatta acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta
     1321 taaaatcatc acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa
     1381 gcactatcat taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata
     1441 taaacttgat gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc
     1501 atcgtcagaa ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa
     1561 cagtaattat ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta
     1621 ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac
     1681 gcatccaatc tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc
     1741 gccactttcc ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac
     1801 tttatgaaga gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa
     1861 ctgaactctc tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc
     1921 acgctggcgc agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc
     1981 ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat
     2041 gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg
     2101 aatgtcgtgt cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga
     2161 agtttttgag aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac
     2221 cctgtggggt aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga
     2281 ccgggtctgc ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt
     2341 tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca
     2401 actggcaagc taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     2461 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     2521 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     2581 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     2641 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt
     2701 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa
     2761 ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct
     2821 acagatggca agaaatagcg cctgccaggc atcttttccg gccattgtcg cagcgctgta
     2881 acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga
     2941 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag
     3001 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg catcatgccc
     3061 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     3121 cccacgcgat cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat
     3181 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     3241 tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt
     3301 ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa
     3361 ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct
     3421 gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact
     3481 gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt
     3541 cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac
     3601 tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc
     3661 acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa
     3721 cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg
     3781 gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag
     3841 cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga
     3901 cacacaaaac taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc
     3961 aaatcttact ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg
     4021 ccagaaacgc taccagctct cccgctgtca tcccttctgc cgagcacgtg tgaaaacgtg
     4081 cctgttcacc aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc
     4141 ctgattcaat catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc
     4201 aaaaaaagcc tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa
     4261 tgcttttatt gtacatttat atttacacaa tatgtaacgt cgatttgacg aagcagccgt
     4321 tatgccttaa cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt
     4381 gaaaaacaga accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat
     4441 gctggcaatg ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat
     4501 tgggctttgg gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt
     4561 tccggcagat accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg
     4621 gctgacgggc ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg
     4681 tgccggtgaa ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac
     4741 cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct
     4801 ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct
     4861 ggtattgctg ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg
     4921 gctggcactg tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcatgcc
     4981 gagtattgtc agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg
     5041 tagtgccatc cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga
     5101 ttcaacaacc tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc
     5161 gttacgcgaa atggtgccct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt
     5221 agccctcgcc acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt
     5281 gtttcagcgt tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc
     5341 gccgttggcg tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg
     5401 tgtggcgctg gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa
     5461 gcacaatcct caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt
     5521 tctctgtggt attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga
     5581 agtggggtga tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc
     5641 aatgtgacct cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa
     5701 ggatcgttac gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc
     5761 ggatgcgcca tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc
     5821 tcttcaaacg cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc
     5881 agcgcaatcg cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt
     5941 gccacgcccc gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca
     6001 acttctcgca agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg
     6061 gtatcggcca tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc
     6121 caacgcggca catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc
     6181 tcttgcggag aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg
     6241 gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt
     6301 aacggtcccg ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac
     6361 atgccggtcc gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga
     6421 gggtgtaacg acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc
     6481 ttattacttt gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc
     6541 acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct
     6601 cgggaagaaa gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc
     6661 tacataaccg ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg
     6721 acgccgatgt cttttaaact ggagcgggaa acgagactcg aactcgagac cccgaccttg
     6781 gcaaggtcat gctctaccaa ctgagctatt cccgtattca tcaagcaatc agttaatcac
     6841 ttgattttat tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc
     6901 tggcgcgatg agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg
     6961 aaacgatcac tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca
     7021 cagagtcagt accgcagcca caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt
     7081 cggacgccac agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat
     7141 ccaggagacg gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga
     7201 aataataatt tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata
     7261 atgctcggtt accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag
     7321 gaaagcacca aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc
     7381 agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag
     7441 ataaaagacc aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg
     7501 gatattaaat tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg
     7561 ctacagagac atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata
     7621 cccggcactt ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata
     7681 tatttcaaca acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg
     7741 gtatttttga ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca
     7801 tcgcgaatat gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca
     7861 ccttccggct caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg
     7921 ccaagtagca gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc
     7981 gcctgcgcaa gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg
     8041 tcaatggctt taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc
     8101 ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca
     8161 aacaccacac aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc
     8221 atccgcttca cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg
     8281 gtagattgct gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc
     8341 agataacgcg ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt
     8401 tctgaaaggg aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca
     8461 cccggtaagg tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc
     8521 acctcgctca gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact
     8581 ttgccctgac gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca
     8641 ataacgtcaa ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc
     8701 gcctgaattt ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt
     8761 tccatacggc taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc
     8821 acatactcga cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc
     8881 agacagcgcc ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt
     8941 tcgcactggc gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag
     9001 ccattcggga acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc
     9061 agacgcgggt gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc
     9121 aaaacgttgt aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg
     9181 gtttctgtat gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt
     9241 ttgcgcgaag cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct
     9301 ttgccgacat agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg
     9361 gttacggttt ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct
     9421 ccgcattaca cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt
     9481 ttagtttact gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct
     9541 gttctgagat ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac
     9601 gttcagacaa actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca
     9661 tttgttgagc gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac
     9721 tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga
     9781 ctttcgctgg taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg
     9841 tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca
     9901 ttagcaccac gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg
     9961 cctctccgac gacttttata ccctttatat cttccagaat gcgtcgtatc cctgcgcgca
    10021 ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga
    10081 taacgctact gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt
    10141 taaaaatggt gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat
    10201 ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg
    10261 tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta
    10321 attaacccga acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata
    10381 ttaacgtatt ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag
    10441 caaagcttat ggtatactcc gccgccttaa catttttcac cgcaaatttt cattgcaaca
    10501 tgacgaggaa aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg
    10561 gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa
    10621 gctgacgcgg gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa
    10681 acccaggctg cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa
    10741 tataaaaccg gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct
    10801 gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc
    10861 atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc
    10921 accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg
    10981 caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca
    11041 ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag
    11101 tcatcattcc acattaaatg gtcctgacta aagttttcag ggttcagcac aggatcaatt
    11161 gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa
    11221 gccactttag gtcgcgtgaa tagtaccggg tggcgaacac ataacgaata gtaatcgtac
    11281 tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc
    11341 tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac
    11401 cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa
    11461 gatttatata taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct
    11521 cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg
    11581 tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt
    11641 ctcgaggaac tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc
    11701 ctgctcgact atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa
    11761 gctcatttcg tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt
    11821 gttcaggact tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat
    11881 cacctcagga cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga
    11941 caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg
    12001 agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat
    12061 gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg
    12121 ctgacgcaac tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt
    12181 gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag
    12241 caatgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac
    12301 ctcgaggtcg ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac
    12361 ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc
    12421 tgcaacgtcg ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg
    12481 gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc
    12541 tggattgtcg cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc
    12601 ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc
    12661 ataccaatac tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc
    12721 agcgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg
    12781 ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg
    12841 agcggcgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt
    12901 ggcgacaggc gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg
    12961 atactgagtt gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc
    13021 agttgtatac tttcttgcat gagcgttccc ggagttaaac gtggggatga taggcgaaca
    13081 gcgcaggcgc gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct
    13141 gactgatacc gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa
    13201 taccttccag acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt
    13261 agccaggtgc aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt
    13321 ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg
    13381 acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc
    13441 cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt
    13501 cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag
    13561 aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct
    13621 cttccagttg ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca
    13681 gatccagcaa caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat
    13741 tttccagcag cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca
    13801 catggttaga ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat
    13861 ctgccgcgag aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct
    13921 catcccgttt gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga
    13981 gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca
    14041 taacagcctc cgatgcgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa
    14101 taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc
    14161 agcaccaaac catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat
    14221 tgcatcattc accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc
    14281 ctgacgggag aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc
    14341 cagcgccgcc agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata
    14401 tttggtcggg tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca
    14461 ctcttcatag ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt
    14521 tttaatggtg ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg
    14581 ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac
    14641 atcaatgcgt ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga
    14701 cgcctcaacg ccaagatgtt ttgccagctg ttgagcaaat tccacttcaa aaccggttaa
    14761 tttgccgtca tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag
    14821 cgtgccgcgc tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat
    14881 gcccgcaacc agcgccacgc ccatcacacc catcaatgcc tgacgtccca gatgtgctaa
    14941 tttcatattc accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa
    15001 cacaaaaaca actccgctac atcttattct tatttaatat atatcagaag caggcaggct
    15061 ggaggatttc tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa
    15121 tgcgatgcgg taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag
    15181 gaaaccgtcc tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg
    15241 gcgcagacga acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag
    15301 aaatttctta aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc
    15361 gcaaaatcgg cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag
    15421 cagcgtgtcc aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt
    15481 aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt
    15541 aagatagcgg cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta
    15601 gtcaaagtta aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg
    15661 tttaatggcc tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc
    15721 aatctctttg agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc
    15781 cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg
    15841 tagcgggttt tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc
    15901 acgccactca tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata
    15961 atcggcaata tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag
    16021 ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg
    16081 cggcacccag tcacggctgc gaagttcatc cagcatagcg ccacggatac gctgcactgc
    16141 gtaagttgta aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc
    16201 aatgccgccc gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag
    16261 gcgcaatgct tcgtgacaca ccagcgggac ataacgctgc cacagcgagt gtttatccat
    16321 tacaccttca gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc
    16381 ggcatgatta tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg
    16441 ttatttgggg gttacgcaaa aagccgaatg taaaaaaccc cgtcggtggc ggggtttgag
    16501 aggcattcag acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct
    16561 tttgccagca cggagttacc ggcctgcagg ataatctgcg ctttcgacat gttggacact
    16621 tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg
    16681 ttgttcaggt tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg
    16741 aatttgtcga tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct
    16801 gcttttgttt cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg
    16861 ctgccgttag tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc
    16921 aacgcctcgt tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg
    16981 ccgttgacgc tataacctac agtagtgaaa tcaacattgt ttcccgaggt tgatacagct
    17041 ttatcgaaag ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc
    17101 gtagtactat taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg
    17161 gtattattgg ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt
    17221 ttcgttaagc gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta
    17281 ccatcactgc tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg
    17341 gtatcaccaa ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta
    17401 tcaaaagagt atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt
    17461 ccgaagccgt tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa
    17521 tcagacgctt tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt
    17581 gcattagcag ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca
    17641 ttcacattaa acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg
    17701 atagtctggc cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg
    17761 ttcacgccgt tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg
    17821 atttcgtcct gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc
    17881 gtcagctcac ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc
    17941 tgtgcaacag aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta
    18001 gaagtaaaac ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc
    18061 aagccagaag acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta
    18121 ttttgagtga tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct
    18181 atattgcaag tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc
    18241 ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt
    18301 tagtcgacga aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa
    18361 cgtaaacttt gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata
    18421 aaaggaaatc gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt
    18481 tcaatccttg atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag
    18541 caatcgtcgt ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact
    18601 ttccagaccg ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc
    18661 agcagcacca ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc
    18721 atcagcgtca cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat
    18781 acgaaaacgg cgatcgccac cagcgacagc aaactcacca ttcaacaagg cggcgacaaa
    18841 gatccgatta ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc
    18901 atcaacaacg caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat
    18961 cgtctgtcag tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc
    19021 ggtgatgatg cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg
    19081 gaggtctcgg tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac
    19141 agcagcaaca ctatcagcga cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc
    19201 acgggcaacc agacgctaac catcactcag gacacctcca aagcgcaaac ggcgattaaa
    19261 gactgggtga atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc
    19321 gccgtagatg cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc
    19381 acgctgcgga cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc
    19441 agctataaaa cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa
    19501 ctggatgccg acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg
    19561 attgttggcg atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt
    19621 tggctttcga caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat
    19681 aagttaacta aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac
    19741 aaagaacaat ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac
    19801 ttaacgcagc agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag
    19861 gtcatgtacg cggcaaaagg cacccaggcc tatgcgcaaa ttggcgtcga aagcaccgta
    19921 atgagcgcca gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg
    19981 gttagagcgc gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca
    20041 aaagcgatca acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac
    20101 gaattaaccc aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc
    20161 aatttacgca acgatgtctc cgcagtcgaa gaggtggaag cattaatgcg taatattgcc
    20221 gatgcctgga aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca
    20281 atgcaccgca tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac
    20341 gcctggcaac ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg
    20401 cggtgcagga gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc
    20461 agctccgccc gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac
    20521 agattcggat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt
    20581 taagtgccta tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa
    20641 tcttaatgag ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct
    20701 tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc
    20761 ggaaggcggt aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg
    20821 tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg
    20881 ctacgactcc tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac
    20941 taaatatggt gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat
    21001 tgcggtgctg ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat
    21061 tcgcgtgcag cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg
    21121 tgaaggctgg acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg
    21181 ggatttcaaa tgttttagcg gtatcgacca tatcgaacac cctgatgaag attgcatttt
    21241 taaaattgtt aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg
    21301 taatttcgat tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga
    21361 gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga
    21421 tgcggtcaaa catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt
    21481 tgcgccaaag ccgctgttta ttgtggctga atactggtcg catgaagtcg ataaactgca
    21541 aacgtatatt gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa
    21601 attccatgaa gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac
    21661 gctggtggaa gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc
    21721 gttgcaagcc ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat
    21781 tttgttgcgg gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta
    21841 cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct
    21901 tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga
    21961 ccatccgaac tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt
    22021 ggtcatgtcg aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa
    22081 taaaacctgg cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg
    22141 cgaagcgacc ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat
    22201 ttaaatccat ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg
    22261 gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca
    22321 taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg
    22381 taacgttagt gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct
    22441 gccactgacg gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt
    22501 ccggactgac cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg
    22561 gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca
    22621 tgagcgcgcc tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga
    22681 ttgtggcatt aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta
    22741 actattattt ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc
    22801 tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca
    22861 cgcctggttg attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc
    22921 cacttactat tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg
    22981 gggcggtcag ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat
    23041 tatccatctg gaaggatcgc cattaacccg catcgacggc atgatgatcc tcggtatgtt
    23101 tggcggctgc tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag
    23161 ccgtatccgc attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct
    23221 ggcaatgggc tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc
    23281 ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct
    23341 gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca
    23401 aaaaccggat caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct
    23461 gggctgggcg ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg
    23521 cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga
    23581 tatgtggatc accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag
    23641 tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg
    23701 accaaatgcg gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg
    23761 cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct
    23821 gggcaacgtg atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct
    23881 ggccaccgac tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct
    23941 ggtgacatat ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt
    24001 cttccgccgt gcggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat
    24061 taccgccttg atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg
    24121 atgccgcaat taaaaaaagg tgagatccta gaagtggtga gcgactgtcc gcagtcgatc
    24181 aataatattc cactggatgc gcgtaatcac ggctacactg tgctggatat tcagcaagac
    24241 gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc
    24301 gggaacatcg ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt
    24361 atgatctcag gaggttgcta tgtgtggacg ctgtgcccaa tcccaaacgc gtgaggatta
    24421 ccttgcgctt ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg
    24481 cagatacaat gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct
    24541 tcatctggat ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat
    24601 taacgcccgc gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg
    24661 tcgggcaatc tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag
    24721 ccttatttta tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata
    24781 ccgtttgagc gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa
    24841 ggtctggtag atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa
    24901 tggatgcggc aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg
    24961 ccagcaaacc agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag
    25021 ggcgcggagt taattcaacc tgtttgttga catcctccac gccctgaatg acgaggcccc
    25081 ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc
    25141 tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc
    25201 gccatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga
    25261 caggcgattt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg
    25321 cccgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc agccctgatc
    25381 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt
    25441 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattacgtg
    25501 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca
    25561 ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt
    25621 gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa aactgtttac
    25681 aggctcaaag actgtgccat ctgacagcga ggcaagttta gccacgccag catccagccc
    25741 gaccattgat gctgaagggt gaaccggagt tgatacttca ctttctgcct gaatactgat
    25801 gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg
    25861 gctgttccgg tagcgcatcc agccaagttt tggcagaaaa atacggctgt tttcctgatc
    25921 gagcttaacg ccctgcggat agcggaatgc atcattctgt caccgctttt tgaatcaggg
    25981 gaacgccgac cgcttctgga aaaagttttt gcaggcctgc tcagggtctt tcagtgactg
    26041 ttgcaatggc tgtgacggag catctttaat ccattgcgtt tcagtggcat ttttccactc
    26101 aaccaatcag gaagccattt tcgcgtaagg gatgtctttg ttaccggcct tatgattctc
    26161 attctgacgt gccagagtac gactgaaaac gaaacgacat gcgcctgcga agcgcctcat
    26221 ttcacgcgcc tgttgaccac cgggtcttaa ccggaatttg aatacctgta gtcgtttcac
    26281 agggcaaatt atactttggt ctgtgaaaaa aactgatatc cgacgcggaa ggcactgtgt
    26341 tttcctgatg catgctcacc gggtctttgt taccaagtac cgacgccaga tttttgacca
    26401 tgacgcgaca gaaaaactac gtacttactt ttcaaatgta tgtgctgatt ttgaagctga
    26461 actggttgaa atggatggcg aaccagagca cgtccatctg ttaattaact atccccccaa
    26521 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
    26581 gacgggatcg gccagatatt gctgccaggt attaccgcaa agatgttttg tggcgtcccg
    26641 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
    26701 aacagcaaac acctggtcag gtgaaaaaac gagccttata tccccgccct gaaggacggg
    26761 cttttacggt gcactggata agcctggaga ttatctacac ctgcatggac atcattgtac
    26821 tatgccccgt acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca
    26881 ttatgcgtct cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg
    26941 gcgctatatt gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat
    27001 catacaaata atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag
    27061 tcaatcataa ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa
    27121 atagtcatgc attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa
    27181 tttaaatgag aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg
    27241 tagtgcagtg acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat
    27301 taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag
    27361 cagagattta caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc
    27421 actacttctc ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca
    27481 aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca
    27541 aaggcaaccg tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg
    27601 gcctgaaatt cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg
    27661 atatcggtgc atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag
    27721 atgtgttcat gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg
    27781 gtctggttga tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg
    27841 taactgaagc taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg
    27901 gtgtaggtgc aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca
    27961 acagcctgaa tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg
    28021 cgaacaacat ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta
    28081 ataaccatat tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact
    28141 tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg
    28201 gtgatcagga cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata
    28261 tgtccacttt tgttgattac aaagtcaacc tgattgataa gagcgatttc acgaaagcat
    28321 ctggcgttgc taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt
    28381 actcaaagat atgttgtggg aggctttgcc tccccaacat ataagtggcg ccctcaagcc
    28441 actttcctta gaagcactat cttgcttctg actatataaa ttacccttta ttttctggga
    28501 cgtttcaacg ccccattttt aataatactc agtgaacaat taacgtatca attagattta
    28561 ataacaacga tatccgtacc taacctgata tctaatgtca taagcatccc tttaattatc
    28621 ccctcaacct cttgtaaatt tcccactctt gagctccaca tagtccaaac gccggatgca
    28681 aaaaaaatca cacgtgcatc tctgaaattg tgatggagga agtgagtatc aattatatgt
    28741 gcttataaac gttcccgatt ggcacagaag tcaccggatt gttcaggctc caataggttg
    28801 agtatgtcgt atttattttc caaaataaaa a
//
LOCUS       DAJTYI010000039        28702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYI010000039 DAJTYI010000000
VERSION     DAJTYI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..884
                     /gene="hisG"
                     /locus_tag="OO564_001684"
     CDS             <1..884
                     /gene="hisG"
                     /locus_tag="OO564_001684"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6652837.1"
                     /translation="LRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAENMPIDI
                     LRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFGGCRLSL
                     ATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPRAGLADA
                     ICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQGVIQAR
                     ESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWETMEKLK
                     ALGASSILVLPIEKMME"
     gene            890..2194
                     /gene="hisD"
                     /locus_tag="OO564_001685"
     CDS             890..2194
                     /gene="hisD"
                     /locus_tag="OO564_001685"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6652838.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2191..3261
                     /gene="hisC"
                     /locus_tag="OO564_001686"
     CDS             2191..3261
                     /gene="hisC"
                     /locus_tag="OO564_001686"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6652839.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3261..4328
                     /gene="hisB"
                     /locus_tag="OO564_001687"
     CDS             3261..4328
                     /gene="hisB"
                     /locus_tag="OO564_001687"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6652840.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4328..4918
                     /gene="hisH"
                     /locus_tag="OO564_001688"
     CDS             4328..4918
                     /gene="hisH"
                     /locus_tag="OO564_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6652841.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            4918..5655
                     /gene="hisA"
                     /locus_tag="OO564_001689"
     CDS             4918..5655
                     /gene="hisA"
                     /locus_tag="OO564_001689"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6652842.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5637..6413
                     /gene="hisF"
                     /locus_tag="OO564_001690"
     CDS             5637..6413
                     /gene="hisF"
                     /locus_tag="OO564_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6652843.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6407..7018
                     /locus_tag="OO564_001691"
     CDS             6407..7018
                     /locus_tag="OO564_001691"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6652844.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7204..8181)
                     /gene="wzzB"
                     /locus_tag="OO564_001692"
     CDS             complement(7204..8181)
                     /gene="wzzB"
                     /locus_tag="OO564_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6652845.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8327..9493)
                     /gene="ugd"
                     /locus_tag="OO564_001693"
     CDS             complement(8327..9493)
                     /gene="ugd"
                     /locus_tag="OO564_001693"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6652846.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(9743..11149)
                     /gene="gndA"
                     /locus_tag="OO564_001694"
     CDS             complement(9743..11149)
                     /gene="gndA"
                     /locus_tag="OO564_001694"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6652847.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11258..11617)
                     /locus_tag="OO564_001695"
     CDS             complement(11258..11617)
                     /locus_tag="OO564_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652848.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12267..12830)
                     /locus_tag="OO564_001696"
     CDS             complement(12267..12830)
                     /locus_tag="OO564_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652849.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13038..13976)
                     /locus_tag="OO564_001697"
                     /pseudo
     CDS             complement(13038..13976)
                     /locus_tag="OO564_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14039..14413)
                     /locus_tag="OO564_001698"
     CDS             complement(14039..14413)
                     /locus_tag="OO564_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6652850.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14417..15565)
                     /gene="wzy"
                     /locus_tag="OO564_001699"
     CDS             complement(14417..15565)
                     /gene="wzy"
                     /locus_tag="OO564_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6652851.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15585..16490)
                     /gene="rfbG"
                     /locus_tag="OO564_001700"
     CDS             complement(15585..16490)
                     /gene="rfbG"
                     /locus_tag="OO564_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6652852.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16483..17373)
                     /locus_tag="OO564_001701"
     CDS             complement(16483..17373)
                     /locus_tag="OO564_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6652853.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17403..18659)
                     /gene="wzx"
                     /locus_tag="OO564_001702"
     CDS             complement(17403..18659)
                     /gene="wzx"
                     /locus_tag="OO564_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6652854.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18675..19220)
                     /gene="rfbC"
                     /locus_tag="OO564_001703"
     CDS             complement(18675..19220)
                     /gene="rfbC"
                     /locus_tag="OO564_001703"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6652855.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19225..20103)
                     /gene="rfbA"
                     /locus_tag="OO564_001704"
     CDS             complement(19225..20103)
                     /gene="rfbA"
                     /locus_tag="OO564_001704"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6652856.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20162..21061)
                     /gene="rfbD"
                     /locus_tag="OO564_001705"
     CDS             complement(20162..21061)
                     /gene="rfbD"
                     /locus_tag="OO564_001705"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6652857.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21061..22146)
                     /gene="rfbB"
                     /locus_tag="OO564_001706"
     CDS             complement(21061..22146)
                     /gene="rfbB"
                     /locus_tag="OO564_001706"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6652858.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(22519..23412)
                     /gene="galF"
                     /locus_tag="OO564_001707"
     CDS             complement(22519..23412)
                     /gene="galF"
                     /locus_tag="OO564_001707"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6652859.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23587..24981)
                     /gene="wcaM"
                     /locus_tag="OO564_001708"
     CDS             complement(23587..24981)
                     /gene="wcaM"
                     /locus_tag="OO564_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6652860.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(24992..26212)
                     /gene="wcaL"
                     /locus_tag="OO564_001709"
     CDS             complement(24992..26212)
                     /gene="wcaL"
                     /locus_tag="OO564_001709"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6652861.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26209..27489)
                     /gene="wcaK"
                     /locus_tag="OO564_001710"
     CDS             complement(26209..27489)
                     /gene="wcaK"
                     /locus_tag="OO564_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6652862.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27669..>28702)
                     /gene="wzxC"
                     /locus_tag="OO564_001711"
     CDS             complement(27669..>28702)
                     /gene="wzxC"
                     /locus_tag="OO564_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6652863.1"
                     /translation="SAVLAGFTFTVVSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRK
                     IYCPGLHFSLASVAPNLRFGAWLTADSIINYLNTNLSTLVLARILGAGVAGGYNLAYN
                     VAVVPPMKLNPIITRVLFPAFAKIQDDTEKLRVNFYKLLSIVGIINFPALLGLMVVSN
                     NFVPLVFGEKWNSIIPVLQLLCIVGLLRSVGNPIGSLLMAKARVDISFKFNVFKTFLF
                     IPAIVIGGQMAGAIGVTLGFLLVQIINTILSYFVMIKPVLGSSYRQYILSLWLPFYLS
                     LPTLVVSYALGIVLKGQLALGMLLAVQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSE
                     KMKMLLRAG"
BASE COUNT         8080 a         6849 c         6206 g         7567 t
ORIGIN      
        1 gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactcacgc gaattactgg
       61 cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca
      121 tgccgattga tattctgcgc gtgcgtgacg atgacattcc cggtctggta atggacggcg
      181 tggtagacct tgggattatc ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg
      241 cccagggtga agatccgcgc tactttaccc tgcgtcgtct ggatttcggc ggctgccgcc
      301 tttcgctggc aacgccggtt gatgaagcct gggacggtcc gctctcctta aacggtaaac
      361 gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa ggtatctctt
      421 ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc gcgtgccggg ctggcggatg
      481 cgatttgcga tctggtttcc accggtgcca cgctggaagc taacggcctg cgcgaagtcg
      541 aagttatcta ccgctcgaaa gcctgcctga tccagcgcga tggcgaaatg gaagaatcta
      601 aacagcaact gatcgacaag ctactgaccc gtattcaggg tgtgatccag gcgcgcgaat
      661 caaaatacat catgatgcac gcaccgaccg aacgcctaga tgaagtcatc gccctgctgc
      721 caggtgccga acgcccaacc attctgccgc tggcgggtga tcaacagcgc gtagcgatgc
      781 acatggtgag tagcgaaacc ctgttctggg aaacgatgga aaaactgaaa gcactgggtg
      841 ccagttcaat cctggtcctg ccgattgaga agatgatgga gtgatcgcca tgagctttaa
      901 cacaatcatt gactggaata gctgtactgc ggagcaacaa cgccagctgt taatgcgccc
      961 ggcgatctcc gcttctgaaa gcattacccg caccgttaac gatattctcg ataacgtgaa
     1021 aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag tttgataaaa ccacggttac
     1081 cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc gaacgcctga gcgacgagct
     1141 aaaacaggcg atggcggtgg cagtaaagag tattgaaacc ttccacactg cgcaaaaact
     1201 gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca cgcgcccggt
     1261 ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg ctcttctcaa cggtattaat
     1321 gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct caccgccgcc
     1381 gattgccgat gaaattcttt atgcggcgca gctgtgcgat gtgcaggacg tgtttaacgt
     1441 cggcggcgca caggccattg ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga
     1501 caaaatcttc gggccgggta acgcctttgt caccgaagca aaacgccagg taagccagcg
     1561 tctggacggt gcggcgatcg atatgcccgc aggcccgtcg gaagtgctgg tgattgctga
     1621 cagcggcgct acgccggatt ttgtggcttc tgatttgctt tctcaggctg aacacggccc
     1681 tgactcacag gtaattttac tgacccccga tgctgatatg gcgcgtcgcg ttgccgaggc
     1741 cgtcgaacgc caactggcag aactgccgcg tgccgaaacc acccgccagg cactgaacgc
     1801 cagccgcctg atcgtgacta aagatttagc gcagtgcgtg gagatctcca accagtacgg
     1861 cccggagcac ctgatcattc agacccgcaa cgcccgcgaa ctggtcgatg gcatcaccag
     1921 cgccggttcg gtatttcttg gtgactggtc accggaatcg gcaggcgact acgcctccgg
     1981 caccaaccac gtgctgccga cttacggtta caccgccacc tgttccagcc tcgggctggc
     2041 agatttccag aaacgcatga ccgtacagga actgtcgaaa gaaggtttct ccgcgctggc
     2101 ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc gcccacaaaa atgccgttac
     2161 tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg tgactattac tgatttagcg
     2221 cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg cggtaacggc
     2281 gacgtctggc tgaacgccaa cgaatatccc acagccgtgg agtttaagct tactcagcaa
     2341 acgctcaacc gctacccgga atgccagcca aaagcggtga ttgaaaatta cgcgcaatat
     2401 gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg cggacgaagg tattgaactg
     2461 ctgatccgcg ctttttgcga accgggtaaa gacgccatcc tctactgccc gccaacgtac
     2521 ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat gccgcacagt gccgacgctg
     2581 gaaaactggc aactggactt acagggcatt tccgacaagc tggacggcgt aaaagtggtc
     2641 tatgtttgca gccccaacaa ccccaccggg caactgatca atccgcagga ttttcgcacc
     2701 ctgctggagc taacccgcgg taaggcgatt gtggttgccg atgaagctta tatcgagttt
     2761 tgcccgcagg catcgttagc tggctggctg gcggaatatc cgcacctggc gattttgcgt
     2821 accctttcga aagccttcgc gttggcgggg cttcgttgcg gatttacgct ggcaaacgaa
     2881 gaagtcatca acctgctgat gaaagtgatc cccccctacc cgctctcgac gccggttgcc
     2941 gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg ccatgcgcga acgggtggcg
     3001 caaattatta cagaacgcga atacctgatt gccgcactga aagagatccc ctgcgtggag
     3061 caggttttcg actctgaaac caactacatt ctggcgcgct ttaaagcctc cagtgcagtg
     3121 tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca accctcttta
     3181 agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc
     3241 ttacgtgcgg agcaagtttg atgagtcaga agtatctttt tatcgatcgc gatggaaccc
     3301 tgattagcga accgccgagt gattttcagg tggaccgttt tgacaaactc gcctttgaac
     3361 cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg ctacaagctg gtgatgatca
     3421 ctaatcagga tggtctggga acacaaagtt tcccgcaagc ggattttgat ggcccgcaca
     3481 acctgatgat gcagatcttc acctcgcaag gcgtgcagtt tgatgaagtg ctgatttgtc
     3541 cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa agtaaaactg gtggagggtt
     3601 atctggctga gcaagcgatg gatcgtgcca acagttatgt aattggcgat cgcgcgaccg
     3661 acattcaact ggcggaaaac atgggcatta atggtttacg ctacgaccgc gaaaccctga
     3721 actggccaat gattggcgag caactcacta aacgagaccg ttacgcccat gtagtgcgca
     3781 acaccaaaga gacgcagatt gacgtccagg tgtggctgga tcgcgaaggt ggcagcaaga
     3841 ttaacaccgg cgttggcttc tttgatcaca tgctggatca gatcgccacc cacggcggtt
     3901 tccgcatgga aatcaatgtc aaaggcgacc tctatatcga cgatcaccac accgtcgaag
     3961 ataccggcct ggcgctgggc gaagcgttaa aaattgccct cggcgacaaa cgcggtattt
     4021 gccgctttgg ttttgtgctg ccgatggacg aatgccttgc ccgctgcgca ctggatatct
     4081 ctggtcgccc gcacctggaa tataaagccg agtttaccta ccagcgtgtg ggcgatctca
     4141 gcactgaaat gatcgaacac ttcttccgtt cgctctctta caccatgggc gtgacactac
     4201 acctgaaaac caaaggtaaa aacgatcatc accgtgtaga aagcctgttc aaagcctttg
     4261 gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac cctgccctcg tcgaaaggag
     4321 tgctgtaatg aacgtggtga tccttgatac tggctgcgcc aacctgaact ctgtgaagtc
     4381 tgccattgcg cgccacggtt atgaacccaa agtcagccgt gacccggaca ttgtgttgct
     4441 ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa gcagcgatgg atcaggtgcg
     4501 tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctgg gtatctgctt
     4561 agggatgcaa ctcctgggac gacgcagcga agagagcaat ggcgtcgact tgctgggcat
     4621 catcgacgaa gacgtgccga aaatgaccga ctttggtctg ccactgccac atatgggctg
     4681 gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag gggattgaag acggcgcgta
     4741 cttttacttt gttcacagct acgcaatgcc agtcaatccg tggaccatcg cccagtgtaa
     4801 ttacggcgaa ccgttcaccg cggcggtaca aaaagataac ttctacggcg tgcagttcca
     4861 cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg agatgtgatg
     4921 attattccgg cattagattt aatcgacggc accgtggtgc gtctccatca gggcgattac
     4981 ggcaaacaac gcgattacgg taacgacccg ctgccgcgct tacaggatta cgctgcgcaa
     5041 ggtgccgaag tgttgcacct ggtggatctg accggggcaa aagatccggc taagcgtcaa
     5101 atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc cggtgcaggt tggcggcggt
     5161 gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg gcgttgcgcg cgtggtggtc
     5221 ggttccaccg cagtgaaatc accggacgta gtgaaaggct ggtttgaacg tttcggtgcc
     5281 gatgcgctgg tgctggcact ggatgtccgt attgacgagc aaggcaacaa gcaagtggca
     5341 gtcagcggct ggcaagagaa ctcgggcatt tcactggaac aactggtgga aacctatctg
     5401 cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc gcgacggcac gctggcggga
     5461 tctaacgtct ctttatatga agaagtgtgt gccagatatc cgcaagtggc gtttcagtcc
     5521 tccggcggca ttggcgacat taacgatgtt gctgccctac gtggcactgg cgtacgcggc
     5581 gtaatagttg gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc catcgcatgc
     5641 tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg tcaggtggtg aaaggcgtac
     5701 agtttcgcaa ccatgaaatc attggcgata tcgtgccact ggcaaaacgc tacgccgaag
     5761 aaggtgcaga tgaactggtg ttctacgata tcaccgcttc cagcgatggc cgtgtggtag
     5821 ataaaagctg ggtatctcgc gtggcggaag tgatcgacat tccgttttgt gtggcgggtg
     5881 ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca
     5941 tcaactcccc tgcgctggca gacccaacat taattactcg cctggccgat cgctttggcg
     6001 tgcagtgtat tgtggttggt attgatacct ggtacgacgc cgaaaccggt aaatatcatg
     6061 taaatcaata taccggcgat gaaagccgca cccgcgtcac tcaatgggaa acgctcgact
     6121 gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt cctcaacatg atgaatcagg
     6181 acggcgtgtg taacggttac gacctcaagc agctgaaaaa agtgcgtgaa gtttgccacg
     6241 tcccgctgat tgcctccggt ggcgcgggca ccatggaaca cttcctggaa gccttccgcg
     6301 atgccgacgt tgacggtgcg ctggcagctt ccgtattcca caaacaaata atcaatattg
     6361 gcgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt taacagaaca
     6421 acaacgtcgc gaactggact gggaaaaaac cgacggtctg atgccggtga ttgtgcaaca
     6481 cgcggtatct ggcgaagtgt taatgctggg ctacatgaac ccggaagcct tagacaaaac
     6541 catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa cagcgactgt ggaccaaagg
     6601 cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc ccggactgcg acaacgacac
     6661 attactggtg ctggcgaatc ccatcgggcc aacctgccac aaaggcacca gtagctgctt
     6721 cggcaacacc gctcaccagt ggctgttcct gtatcaactg gaacaactgc tcgccgaacg
     6781 taaatatgcc gacccggaaa cctcctacac cgccaaactg tatgccagcg gcactaaacg
     6841 cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca ctggcagcaa cggtacatga
     6901 tcgctttgag ctgaccaacg aggcatctga cttgatgtat cacctgctgg tgctgttgca
     6961 ggatcaggat ctggatttaa cgacagtaat tgagaacctg cataaacggc atcagtgagt
     7021 tacgggctga gcaggtgtga tgttgttgtc acacccggag ccattgcccg atgcgcaaac
     7081 ttatcgtgcc tacacattat ctcaacacac gtaggccgga taagatgcgc cagcatcgca
     7141 tccggcaaaa aaaacgggca tggtgtcacc accctgcccg tttctcttaa atgcacaata
     7201 atattacttc gcgttgtaat tacgcagagc gttacgcccc aacacaatcc ccgcgccaac
     7261 catgccgccc agaagcactg ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg
     7321 aataggtaac gtcggtttca tcacatagcg gtaagcatga atatcaagat catcaaactt
     7381 taatttttca atatccaaca ggttttgacg tgtctgatag tagtttggtg agaacaccaa
     7441 cggacgagtc gcttcatgct taatcatcga ctccagcgct tcgctcccta gaaggaacaa
     7501 cgtatcttgc gtcacatctt cagtctgctg aacctgtggc tttgtcacct gcgcctgatt
     7561 cgcatactgc aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcgac
     7621 cacttcctgg gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcctt
     7681 gagatccttt tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa
     7741 cttcatttgt gcgccctctg cagtttgccc aacataagaa acagtcaatg gtaattgttg
     7801 gttcttaaca gaaggttcga tggtaagttt ttctggctct tcctgattat ccagcgtttc
     7861 tgctaatgca gagaaggcag aactgaagcg accaattaac gtctcctgca aatccgatac
     7921 tttcggtgca gcctgaccat agataacatt catggcattg ttatagccag caatttgccc
     7981 cacgtcgggc tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata
     8041 accaatagcc agggcaatag ccacaatgac ggaaatgata attgtcatct tgccacgcca
     8101 caactgcact agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac
     8161 attattattt tctactctca tcattaccct aactgacgaa aatttccaag tccgcatagt
     8221 ttagcgataa tccccgtgaa tgctatagga taagtgataa aaataatgaa ttattgcgga
     8281 ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa
     8341 agagatcgcg ggtgtagacc ttatctgcca catccttaag ctcttctgcc atacggttgg
     8401 agataatgac gtcggcttgt tgtttgaagg tggcgagatc acgttccagg cgagagttga
     8461 agaatgagtc ttctttcatc accggctcgt agatgatcac ttcaacgcct ttcgccttga
     8521 tacgcttcat aatcccctga atagaagacg tacggaagtt atctgacccg ctcttcataa
     8581 tcagacgata aatacccacc acttgcggtt tgcgtgacaa aatggcatcg gcaataaaat
     8641 ctttacgcgt gcggtttgca tcgacaattg ccgagatcag gttattcggc acagactggt
     8701 agttcgccag taactgcttg gtatctttcg gcagacaata accaccataa ccaaacgacg
     8761 gattgttgta atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag
     8821 tattcagacc taaactttct gcataactat ccagttcatt aaagtatgct acgcgcatcg
     8881 ccagataggt attagcgaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg
     8941 tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt
     9001 ctgaacgctc accgatgaca atacgtgacg gatggagatt atcgtaaagg gctttacctt
     9061 cgcggaggaa ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctg
     9121 cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa
     9181 ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat
     9241 agtcggttgg agtggcgatg atgacataat cagcatcccg gtagtcttca tttttatcta
     9301 atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa
     9361 taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa
     9421 cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa
     9481 tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc
     9541 atcgtgataa gatgctaaaa gttttaacgc tacgttgtca gatggtcaac gcaacgaaaa
     9601 acaattcaga ttttctttat aagaatagct gatgtttatt aagttaatat taagcagtga
     9661 aatttagtct ataagatatt tggcaaaaaa aagcccggtg caatatacgc cgggcctcaa
     9721 ttttattgtt ggttaaatca gattaatcca gccattcagt atggaacaca ccttctttat
     9781 caatgcgctt ataagtatgc gccccgaaat agtcacgctg tgcctggatc aggttcgcag
     9841 gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc agcggcgaag gtcggaaccg
     9901 ggataccgtt ctgtactgca taagcgacga catcgcgcag cgcctgctgg tagtcatcgg
     9961 caatttgctt gaagtacggt gccagcagca ggttagcgat ctgcggattt tcggcataag
    10021 catcggtgat tttctgcagg aactgcgcac ggatgatgca gccagcacgg aaaatcttcg
    10081 cgatttcgcc gtagttcaga tcccagttgt attcttcaga cgcagcacgc agctgagaga
    10141 agccctgagc gtaagaaacg attttgccca gatacagcgc actgcgaact ttttcgatga
    10201 actcagcctt gtcgcctgct gactgagctt gcggaccaga gagaacttta gatgcggcaa
    10261 cgcgctgatc tttcagagaa gagatataac gtgcaaacac agactcggta atcagcgaca
    10321 gcggttcgcc gagatccagc gcgctctggc tggtccattt accggtgcct ttgtttgctg
    10381 cttcatccag aatcacatca accaggtagt taccgtcttc atcttttttg gtgaagatat
    10441 ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt attccactcg gtgaaagtct
    10501 gggccagttc ttcattagat agattcaggc cacctttgag cagagaatac gcttcagcaa
    10561 tgagctgcat atctccgtat tcaataccat tgtgaaccat cttcacataa tggcctgcgc
    10621 cgtcggcacc aatataagta acgcatggct cgccgtcttc tgccacggca gcgattttag
    10681 tcaggatagg cgcaaccagc tcataagcct ctttctgccc gccaggcata atagaaggac
    10741 ctttcagcgc gccctcttca ccgccggaaa caccggtacc gatgaagtta aagccctctg
    10801 ctgaaagctc acgattacga cgaatggtgt cctggaagaa ggtattacca ccatcaatga
    10861 tgatgtcacc tttatcgagg tatggtttca gggaatcaat cgcagcatcc gtgcctgcac
    10921 ctgctttcac cattaacagg atgcgacgag gcgtttccag agattcaaca aactctttca
    10981 ccgtatagta aggagccagt ttcttgcctg gattttcagc aatcacttct tccgtctttt
    11041 cgcgggagcg gttgaaaata gagacggtat aaccacgact ttcgatgttg agcgcaaggt
    11101 tgcgccccat cactgccata ccgactacgc cgatctgttg ctttgacatt gtttactcct
    11161 gtcttctata ttattaaccg tctattaacc gttatattat taaccgtcgt gattttcatc
    11221 ttctaatcaa ggaatacata tggttacagc atgcttctta ctttatagcg ataagtatgc
    11281 cattgggtgt atccataaga actttgaatt ttggatactt ttctggatta aaattagcat
    11341 agcttcgttt agatataaca ataaacgaac tattaaaaga tttcatcttc tctattaaat
    11401 catcctgtgg gattacctca cactcgggat tactaatagt ccaatcacta tatcttgcta
    11461 atccaaagac gtcaccaatt gccatccctt tatagaagta aataccatcc tccaagccta
    11521 attggataag attatctcca tataactcac tatattcact tgctttcttc atcagttcat
    11581 agctatagcg actttctaag cgtgaattaa aattcaagta ttcagaataa aaatatttga
    11641 attcaaaatg caacgatcgc cccgaaaaaa aaataataca cacaaggata aaatatcgtc
    11701 ccaacttgta atttgtaatg ttgatatctt ttattaaact tgcaattgag atcgcaacca
    11761 gcaatactga caatgggtaa atgggtgata aatacctgtc accttgcgta acagaaaacc
    11821 aaaataagac atatgttaaa acaatcgaga aaaataccca aagtatttta ttattcctaa
    11881 gtgtaaagac agacagtata gcataaaaaa caacatcact gccaacttta ttaattgctt
    11941 gttgcaaatc taatgtggca ggacttatac catgtcgagc ttgttctgct gtttgaactt
    12001 gtagatcaat ttcattccat aggtagtgac caaatatatt tcctccagca ggactaaaag
    12061 gatcaccaga gatccaaaaa ctacggacat accaataaat gccaccaaga attgttgcca
    12121 agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc tatcaatata acaaatatga
    12181 tggctccata tatggcacca aatagtttta cgccacaagc aattccaata aaaaaaccac
    12241 ataaaaaaat taattcattt tgtgttttat tgttttctga gcgaatatat ataagaatac
    12301 tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg cataaccaag atgatcttta
    12361 agtggattga tagtaaatag taggattgta gcaataatgc taggataata agttttcgta
    12421 atttcccatg aacagccaac aagtccacag gctattacaa aaaaacagat atttgctaaa
    12481 aactgagcta accttacatc tcctaactgt aaacctaaag ccattaaaag atctgcattt
    12541 tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa ttgcatggtg ctcaactaat
    12601 gaacgagcca aaggtaagtg atacatagta tcatcccaat caccgggagc tcgcagggac
    12661 gataacaatg taaccacaaa aatcaaaaca agaaataaaa taacaaacag cgtactgaac
    12721 tcaatattat ttattgccag ccatctaaat tgctgacgtt taataatact taaaacccaa
    12781 cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt gcccgaccat gcccagaaag
    12841 taaattatca aaatatttaa agacatccct atgcataggc aataaaaaaa taaagaaaat
    12901 ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca atacaatatt accaatgaca
    12961 aaaaatgtca ttccaaaagc aaaacaatac acaacataca aatacaacgg caattcaatc
    13021 atagtaaaat actctattta cattatcagt ttatagtttt tttcagaaag ataaaataat
    13081 gccttcattt cacgtctagt tgttgtattt gaatgcaaaa taatgccaca tacaaatgcc
    13141 attattgacg ataacatgat ggaggcagtt aaaaaggcag tcggaaatct tctcaccaaa
    13201 cctgtatcaa tataatcaac aatgatgggc aatcctaaaa ttatagacaa agcagcaagt
    13261 aaaacgccga taatactaaa aaagtatagt ggccgttcaa gcgagtaaag tttaattatt
    13321 gttttcagaa tcttgaaccc atcactccaa gttgatagtt tgctcacact cccttcagat
    13381 cgctcgccat attttgtatt aacctcacca tacttcatac gtaattctaa agcatgaatt
    13441 gtaagctcag tttcgatttc aaatcccctt gatagacatg gaaaagattt aacatagcgt
    13501 ctggaaaata cacgataccc cgacaacata tcagtaaaat tccctttaaa gatagtctga
    13561 acagtaccag tcaacagttt atttcctcag cgatgccctt ttctgtatgt attttgatct
    13621 ccattctctt gacgacatcc tacaaccata tccaattgat tttttatgag atggttaatc
    13681 atttctggag cagaacttgc atcataagtg ccatctccat caaccattag ataaatatcc
    13741 gcatctacat ctgagaacat acgtcttacg acattcccct tgcctttcag aggaacagag
    13801 tgaactgttg ctccagcatc ctctgccact tgtgctgtac tgtcagtcga attattatca
    13861 aatacatata tagaagcagt tggaatattc gttttgaaat catttatgac ttttgctatt
    13921 gccttctgct cattataaca aggaataatt acagcgatat taaaagagtg atacataagc
    13981 ccctcatata aactatttag ctaatgtgca agtaattgtg tttttaacaa atcgtttact
    14041 aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac caaactaaaa agagaaaagg
    14101 tgaacaatgt aaatattgga gataaagcta agagttcaaa taaaacgccg aataaataac
    14161 tcatcgcccc cataaaaaat ataaatataa aatatctagc cttggttgca gatactttaa
    14221 atgagcatct tgcatttgca aaaaaagaaa aggttgctgc acatataaaa ccaacaatgt
    14281 tacttcggcc ttgaccaaaa gctaaattat tataacacgc ataaaatacc ccccaatgta
    14341 aagcagtatt gacaattcca attaaaaaat aactaaaaaa agaactggtc aataacttcc
    14401 ctatttttaa catcctttat tttgctccag aagtgaggtt attactaatt tggatatttt
    14461 ctatagaaaa tacccataaa actacaacgc ctataataaa aattcccatg ggtgtaaaaa
    14521 gaaatggatt agttatagaa acggtaatac tgaaaaaaag tattaacgac atcattaatg
    14581 atgtttttgt actatttaaa aaatagcctt tcaaaaagat aagcaacata ggaactaatg
    14641 atataaatac accaataacg cctgttttct gaagtatctc aagaggtact atttcaatcc
    14701 ttaatcgatc taatataaat gaaccaaatc ctcttccaaa caagaacgtc gctaaatcaa
    14761 catttttata ataaaaatat aaatcattaa atctaacaga gtcagaatct tcactgcgtg
    14821 atccgaccat gtaaagcata attataaata gaacgctgcc caatattatt ataatagtat
    14881 ttaaatatag cttatgtaat ttaaaactgg ctaataacag tgataatgtt gtaaaaagta
    14941 atacacctct tgattctgtc aataatatcg caagcactat tatgagacat tttaaatatt
    15001 ttttgtcgac aaaactgata atcgcaccga caccaaaaaa gtagaaggat ttggaaaaaa
    15061 aagccccatc gggacgaaaa aagaactcgc tagtcaatga taagtgttca taaattaaat
    15121 taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga aatatataac agtgatccat
    15181 ataatgcaaa tgcattaact attttaataa aaatctcaca taaaaatact tttaaagtat
    15241 atcttagtgt aaatagatat ataagtgaca gcataaaaag gtatggttgc acatcaaaca
    15301 gtatatcatt tattttatta ccagctaata agccgacaaa gaagccataa aaaggaaata
    15361 aaagcaacca tataaaaaga ctgacacatt tttgggtgat tgaaaaattg aatgtaacta
    15421 aattaaaaat taaaagtata aaactaaata caaataataa atatcgcaaa gggaatattc
    15481 ccaatggctc cagtaatctt ccaccaccac caataatcag ttcaatacat aaaaatgtta
    15541 taaaaatttt atttatatta ttcatattcg taaggtgatg ttttttaaaa tatgtttaaa
    15601 atcaacatta ataaaatata aattttatca gcctttttcc gccttaactt ccaaaaactt
    15661 aaagccaaga tcaaattgcc tatataacct ttacccaatt gattattcac aaagctatca
    15721 tcagctaagg cattagctat tttagttact tcttttcgat accacttgtt tctcaccaaa
    15781 ccaagccgtt tgtattttgc tttaaaacta atgtttgctc ccacttggtt gttttcatgc
    15841 tgtctgtaca gcattgttgg ttttctatca atataccacg aataatttct cgtccgcgca
    15901 aatgaatata aaaaccaatc atgcagacag atatcttgtg cagcattttt attatttatg
    15961 atgaacttct taaattcaat aagcgtttct ttttttataa catatgtaca ccccggacct
    16021 gctgcttcaa aaaaataatc gaattgagtt tgcgactgag ccttatcaac taaatttttt
    16081 tgccctgatg gataatatgc aattacattg ctggaataac aattcgcatt attaaatacc
    16141 aagcagtcaa ttgcattttt tattttatct ttataccaaa tatcgtcctg atcactaaaa
    16201 cagatataat catagttttc aagatcgaca tctttaatta atctataaaa atttttacct
    16261 gcccccccaa acttattgac gcttggaagt atatttataa ttgagggata tgataattga
    16321 aattcttcac aaatatttaa tgtattatca gttgataaat catctgatat aaaaattgag
    16381 atgtctacat ctttttgttc aataatactt tttaattgtt cctcaatcca gcattcgcca
    16441 ttatacgtag ccataagtac agctactctt ttttgatcag aactatgcat tataacgacc
    16501 gcccccagta attcctctta ttccatccag cgtagccaaa atatgttttt tcatatattc
    16561 aattttattt ttttgagttg cgataattaa agtcgtatga attaaagaaa aaacaacttc
    16621 cctaatagag agtaaaagag gcacatgatt ttttctaagc aaaagaaatg catttcttac
    16681 tcgataataa cgacgtactg gcgagtgaat tggtatgtta atcccacaga tttttactga
    16741 cttatcccca atagcatgaa ccatttctat atcaggaatg atatgaacta atatgccata
    16801 gtttaagcat ctaagacacc attctgtatc gacataatca ataaaaagtt cctcatccat
    16861 cattccaaca atctcaaaaa cttcttttga aaccatggtt ccggatgaga ttgttactga
    16921 acttttaaac ggtgtctgtc cttcagtgac atgaattttc tctcgaagac catttttttt
    16981 gatattacaa ataggataat aatgtgactt atctctatca ataaaaactg ggccagctat
    17041 ttttgcattt tcattatcca tatagcagga aagttttttg ataaactcat tgcgaatacg
    17101 gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt tcaagagaat atcgaatccc
    17161 aatattttgt gcttttgcta taccttcatt cctgccaaga caaattaaat gaactttttc
    17221 aatgtctgct aatttatctt tcaaggtgaa attagtccta ttatcgacta ctacaacgta
    17281 acaattttgc tcctttagtt cagtgatcag ttcattcaga tttttaagtt cgggattata
    17341 tgtcacaatg acagcgtaaa tattactatt cattatccaa gtgactcagt aattggttaa
    17401 ttttagtttt catatacaga acacattcta gtataaagcc attgtaataa cagagcatct
    17461 aaaattacac gtatgctcca ggatattgcg gcacccagta acccatattt ttcgaccata
    17521 aaatacaaaa gggctaaata aggaaccaat tcagcacaat gaatcaaagc agtaatttta
    17581 gattttccta atgcctggat tgcagagaat ggtatcattg ccagcgcatt aaagaaaaaa
    17641 ccgatcaata acacatttaa tatattaaag gaattaattt ggtaattctc accaaaccat
    17701 attttcaata caagccctga atataacaag cctatgatta tcaccggcaa acagattaga
    17761 aacattaaaa gcaatgattt atttacatta cgtttaaatt ctttaaagtc tttgatgtta
    17821 cttaaccttg gaaacactgc cctcccgatt gctgcaggta ttattcctaa ttttagaata
    17881 acctccgctg gtgcagaata aaatgcaact ttatctgccc ccataatatt tgacactata
    17941 aatcgatcaa aatacaccat gaccggactt atgatattac ttactgtcat ccaaccgcca
    18001 aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa cccccgaaat tttaatatca
    18061 ttcctgacat aataggcaga gactaaaatc gaaataactc ttgcaaatat taaaccagca
    18121 accgctgccg acaatgtagc actataaaaa acaaatatgg ccggaattcc cgcgatgcaa
    18181 gagcttgata tagatttttg aatatttaca atgccaaatt tttcatcccc ctcaagaatg
    18241 gctgaccata attgatttag aataaataat ggaatgcaaa tagccaacag tttaaatgct
    18301 agttgtatat cactatgttc aacaccagaa atttttagat aattaacaat tccatcagag
    18361 aaaatcaata ataaaaaagc accaaagcat gaaaatagca ccaaaaaaga tgtacttgtt
    18421 gaaattacct tggttctttc atgatgatta tcacgatgaa ttgcaatttc tctaataaca
    18481 gagcgagtca ggcctacatc aaaaattcca gcatacccaa caagtgcaat tgcaattgta
    18541 taaacaccga aaccttcagg ccccaaacct cgagcgagaa atcctagcga ggggatagca
    18601 attaaagttg gtattgcata gccaaaaagg ttccagacac tattttttat tatgctcatt
    18661 attacatttc tttatcatat aaaataatca gcatctacaa atttttgacc attaatatct
    18721 ttatcagaaa gcaatggatt ttgaaccggc cattctatat ttatactttt gtcgctccat
    18781 aaaatcccac gctcttgcat ttttgaatag aaattatttg ttttgtagac aaattctgct
    18841 tcatcactaa gaacgcaaaa cccatgagcg aatccttccg gtatccataa ttgacgctta
    18901 ttctccgccg acaaatttac accaacccac ttgccaaatg ttggtgatga tttccgaata
    18961 tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta ccagttttcc ttgagcataa
    19021 ggttccagct gatagtgcag tccgcgtaac acacccttag ttgatttaga atggttatcc
    19081 tgaacaaact caacctttcg tcctacagcc tcttcgaaaa ctttctgatt gaagctttcc
    19141 ataaaaaagc cccgctcatc accaaatact tttggttcaa aaattagcac atcaggaatt
    19201 tcagttttaa ttacgttcat tttattaata acctttaatc attttcagca gatactgacc
    19261 ataagcattt ttcttcagcg gctcggctaa taccttcact tgctcagcat caataaagcc
    19321 tttacggtaa gcaatttctt ccgggcagga aactttcagc ccctgacgct cttcaattgt
    19381 tgcaatgaag ttgcttgctt caataagact ttgatgtgtc cccgtgtcca gccatgcata
    19441 accacgcccc atcatagcga cagacaaacg tccttggtcc atataaatac ggttaatatc
    19501 ggtaatttcc agttcgccac gtgcagatgg tttaaggttt ttcgccattt ctacaacgtc
    19561 attgtcatag aaataaagcc cagtaaccgc atagttactt tttggttcca gcggtttttc
    19621 ttccagacta attgccgtac cgttatcatc aaattccacc acaccgtagc gctcaggatc
    19681 atttacgtga taggcaaaaa ccgttgcgcc actctctctg ttaacagcgg tatccattaa
    19741 tttcggcagg tcgtgaccat agaagatatt atcaccaagt accagcgcac aatcatcacc
    19801 acctataaat tcttcaccaa tgataaatgc ttgcgccaga ccatccggac tcggttgtac
    19861 tttatattgc agattaagcc cccactggct tccatcacca agcaattgtt gaaaacgtgg
    19921 tgtatcctgc ggcgtactga taatcagaat atctcgaata cccgccaaca taagcgttga
    19981 aagcggataa taaatcatcg gtttatcata aatcggtagc agttgtttgc tcacagccat
    20041 tgtcacagga taaagacgag tgccggaacc accagccaga ataatacctt tacgcgtttt
    20101 catttcacta ttccttttaa ttcatctcgc tctggcatca tgagcgagat gcaataattt
    20161 gttaaattgc cgtggtcgta aataattcgt tgagcatacg tttcacgccc acctgccagt
    20221 caggcaagac aagcgcaaag ttctgctgaa atttttcggt attgaggcga gaattatggg
    20281 gacgacgtgc tggtgtggga taggccgttg tcggcacggc attaagtttg ttaagagcaa
    20341 ggttgatccc cgctctgcgc gcttcttcaa ataccagagc ggcataatcg tgccaggttg
    20401 ttgtaccacc agcaaccaga tggtacaaac cagcaacttc tggtttattt gcggccacgc
    20461 gaatggcatg agcggtgcaa tcagccagta attcagcccc cgttggtgcg ccgaattgat
    20521 cgtttattac agccaactct tcgcgttctt ttgccaggcg caacatcgtt ttggcaaagt
    20581 tatttccttt accagcgtat acccagctgg tacggaaaat aagatgcttt gcgcaatgtt
    20641 cttgtaacgc tttttcccca gctagcttgg tttcaccgta gacatttagc ggtgcggttg
    20701 catccgtttc cagccatggc gtgtcaccgt tgcctgggaa aacataatca gtggagtagt
    20761 gtataaccca ggctccgact tcattagctg cttttgcaat cgcttcgacg catgtcgcgt
    20821 taagtaattg cgcgaaattc ggttctgatt ccgctttgtc tactgcagtg tgagcagcag
    20881 cattaacaat aacatcgggg cgaatttttt tgacggtttc agccacacct tcggggttgc
    20941 tgaaatctcc acaataatca gtggagtgaa catcgagcgc aatcaagtta cccagaggag
    21001 ccagagcacg ctgcagttcc caacccacct gccctgtttt gccgaaaagc aggatattca
    21061 ttactgacgg ccctcatagt tttgttcaat ccacgactga taagcaccgc ttttcacatt
    21121 ctcaacccaa tttgtattag ccagatacca ttccaccgtt ttacgaatcc cgctctcaaa
    21181 cgtttcttgc ggtttccagc ccaattcgcg gctaatttta tcggcatcaa tggcgtaacg
    21241 gcggtcatgc cccgggcgat cggcaacata agtaatttgc tcacgataag atttctcttt
    21301 cggtacaatc tcatccaaca aatcacaaat agtgagcact acgtcgatgt ttttcttttc
    21361 gttatgtcca ccaatgttat aagtttcacc cgctttacct tcggttacga cggtatataa
    21421 cgcacgagca tgatcctcta catacaacca gtcgcgaatt tgatcccctt taccataaat
    21481 aggtaatgcc ttaccttcca gagcattcag aataaccaat ggaataagtt tttccgggaa
    21541 gtgataagga ccataattgt tcgagcaatt agtcacaatg gttggtaaac cataagtccg
    21601 cttccaggca cgtaccaaat gatcgctgga agctttagac gcagaatacg ggctacttgg
    21661 cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg ttattatttg cttcatccgg
    21721 atggggtaag tcaccataaa cttcatcagt agaaatatga tgaaagcgga aacttttttt
    21781 cttttcatca ttcagagcag accagtaatt gcgcgccgct tctaaaagaa cataagtccc
    21841 cacaatattg gtttcaataa atgccgcagg gcccgttatt gaacggtcaa cgtggctctc
    21901 tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg aaaatacgcg acatggctac
    21961 agcatcgcag atatctgcat gctcaaaagc ataacgttca gaatcagaaa catcagcgag
    22021 tgattctagg ttcccggcgt atgttaattt atcgacatta acaacactat cttgcgtatt
    22081 atttattatg tgacgaacaa cagcagaacc aataaaccct gcgccaccag taacaagtat
    22141 cttcactttt ttattccata tagctataga gcatgctgtg aaatagactg ctccagattt
    22201 gattaataga ggtattaata cacgctaccg cccctggctt aacagctacc agtgcactgc
    22261 atgcatctca acgatgtggc gagcgttagc cactcgcgcc aaatccgaaa aattcaaacg
    22321 ttaattgtct taccaaacag ccgtagaaac aaggaaaatc ctggaaaaat ttgaataata
    22381 tgagctatgt aacttcttgt ttttataggc tttataaaaa cggcaacggc aggaaaattc
    22441 aatgagcaaa acttatgcca cgaatcttca ctgccgttat aattttctta tcaaccgtta
    22501 catccggtca gattttcatt attcgcttaa cagcttctca atacctttac ggaacttcgc
    22561 cccttctttc aggttgcgta ggccatactt cacaaacgcc tgcatatagc ccattttttt
    22621 gccgcagtcg taactgtcgc cggtcatcag cattgcatca acggattgtt ttttcgccag
    22681 ctcggcaata gcatcagtca gctgaatacg tccccatgca ccaggctgag tacgttccag
    22741 ttccggccaa atatcggcag aaagcacata gcgacctacg gccatgatgt ctgagtccag
    22801 cgtctgcggc tgatccggtt tttcgataaa ttcaacaata cggctgactt taccctcacg
    22861 gtccagcggc tctttagtct ggatgacgga gtattcagag aggtcacccg gcatacgttt
    22921 tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca atcatggcag caaggttgta
    22981 acgtagcggg tcggcgctgg catcgtcgat cacaacgtct ggcagtacca cgacaaatgg
    23041 gttgtcacca atggcaggtc gcgcacacaa aatggagtgg cctaaaccta aaggttcgcc
    23101 ctgacgcacg ttcataatgg tcacgcccgg cggacagatg gactgtactt ccgccagcag
    23161 ttgacgcttc acgcgctgct caaggagtga ttctaactca taagaggtgt cgaagtggtt
    23221 ttcgaccgcg ttcttggacg cgtgagttac caggaggatt tctttgatcc ctgcagccac
    23281 aatctcgtca acaatgtact gaatcattgg tttgtcgacg attggtagca tctctttggg
    23341 tatcgcctta gtggcaggca acatatgcat cccgagaccc gctacaggaa taactgcttt
    23401 taaattcgtc attatttcat ccacctgtaa aatggttgct gaattatagc ttgttcgatt
    23461 tttttcgcca gcatcaatta ccctgaattg attactgaat tacttgtgat gttacgccgc
    23521 ttcgttgtgg attgcagtag cattgttcct aagtatggct ccatttttcc aggaatggtc
    23581 gcaaatctac tccctccgtt ccggcaatct aaagttaatc ttttccacat taacaatatg
    23641 gtgattaatc ctgtcgatat cgacggagct ttgccctctt tcattcaccg catgaacatt
    23701 tgcaagagac agcagtgttt cttttttcgc cataaagacg ccgcgaacgt ctttgcgcat
    23761 gtcgaagttc atgctcaatg ctggtccaac tgaggattcc tgcatcacat tgatatttcg
    23821 tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag gcacgcttca tctcaatgtt
    23881 agttagcgac acaaaggaga cggcattccc ggcggagatt tggatgccgc gcaatttata
    23941 agcaagatgg gtattatcca gttgaatatt attcactcgg aaattttgcg gtatcgagag
    24001 atatttgcct ttaattaccc catagccgat taacatcccg gcgctattaa tcatttcaat
    24061 attatcaatc acgaaattgt cacaaccgta aatagcgact gtcgcgttat caatgcccgc
    24121 tttcttactg aaatccggcg tgatattgcg ggcattgata ttacgaataa caaaatgttt
    24181 accattttca acatggttca actgccgaca atccgatccc gtgatattcg ccacgacaaa
    24241 gtttttcact gcctggtctt ccgggtagtt gttatcataa gtgcttcccg caaggcctat
    24301 gccgatgccc cagttgattt tgccgttggt acagttgata cgctcgatga catggtcaga
    24361 tatcaaaata tcactgtcgt taattgccac gttccattca atggcgtcgc cctgtaagtc
    24421 gctgaactta cagttggtaa tgttggcacc gataatctgg ttatgaaatc cctggcgtaa
    24481 gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg gtcaggttgc gcatgacccg
    24541 tttgtttttg ccgccaatat aaatctgcat taccgggcca aagccgctca tcgccagccc
    24601 tttgatggtg cagtcagaac cacgcacatc cagggtaatg ttatgcatac cgccgccctc
    24661 tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc cctctgccgt tgccgcgcag
    24721 gcttccaagg atgtgtaacg ttttgccggg agggatgaag atgccggtgt tgatattgtc
    24781 acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg ctgaaggctt gtttaaatga
    24841 ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg ctttgtcggg cgggaagtgc
    24901 gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt gccgtcagga aggtgcgtcg
    24961 ggagagtgtt ttaaatggca tacgttctcc tctataaagc ctgcagcaag ctggcgagtt
    25021 ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt ttcgcgcgca cgtttgacga
    25081 ccggagccag ttcgtcggta tccagttggc taaacgccgc cagacgttgt gccagtgcgc
    25141 gagcatcgtt ctcaggcacc agccagccgg atttatcggc ctccaccagt tcaggaattc
    25201 cgctatgcag agtagaaacc accggaatac cgaccgccat cgcttccatt agcgccaccg
    25261 gaatgccttc catatcgcca tccgcgcccg ttaccgatgg caacaggaag acatccgcgt
    25321 cgtcgagcat ctccttcact tcatagctcg gtttaaagcc cggcatctct accacatctt
    25381 ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc ccacaggcca atgccgagga
    25441 tgcgatagcg aaatgccacg ccctgctctt tcaactgacg gcaggcttca atcgccacat
    25501 gcaggccttt tttctcggtt aagcgtgcga cagatatgat ttccagcggc gttgcgggcg
    25561 ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc catacgcgat acggcgattt
    25621 tttctctggg gcagcccatt ttttgcagcc ttccggccca cagatcgctt atcggtaaca
    25681 tcaggtcgcc acggcgaaac agttgttgat attcgggagt gtagtggttg agcacttccc
    25741 gactggagat atcaataccg tggaagatgg tggcaatttt gccgcgaatg atacccagtt
    25801 cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg agcgatgaac acatcggcgc
    25861 gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat caggttccgc gactcggcac
    25921 catagcgttt gaggttgagc gcatgccagg tatttttacg atgaatgcca cgtaaggtct
    25981 ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg ttcgtcctgt aaccagcggg
    26041 ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt gttctctgta tcgccttttt
    26101 gcaaagcgac aatctccact tcaaagccca tatcaataaa cgcggtaatt tgattgagga
    26161 cgaaggtttc cgacgacagc gggaatttca ataagaagaa gccgaccttc atttcacctc
    26221 cccgatgcgc tcaagcacag actgcaccat ctgcattccc gtctgacgtt cgcgactgac
    26281 ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct aaggtatccg caatcatcgc
    26341 ttgcaggctg ccgtccagta aatgacggat atcaatcgcc atctctggca gtcccagctg
    26401 ctgcataatc ccggcggatt tatgttcata gttgatggca atcgccggag tagcaaagtt
    26461 cattgagata atggcggagt gcaggcgcgt accgacggtg agttcacagg ctccgagaat
    26521 tttgcccatt tccagatcgt tgagttcatc catcactacg tggtaacggg caggatcgct
    26581 gatgtgctgg cgcagattga gcgccaccat gcggtcgtct ttgttatagc tgtcgatacc
    26641 cgtacaggtg gagagcgcaa tcacctgata gccttcatcg agaatgcgat tgaccacccc
    26701 ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga cgtttgtcga acggtgccag
    26761 ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca acgtccagcc agtgttgaac
    26821 ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc agccacgcgg tatcgacgcc
    26881 atgttccact tttgcggtgg tgatattgct gcgtttcatc aaatcaaggc tgaccgattc
    26941 gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc gccagttggt taaattgctc
    27001 atcctggaag gggccgacgc tgtgaccaat cataaacagc ggctttttcg ccataaatgt
    27061 gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg acaaaaaacg atccgccgac
    27121 ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag tcggtgaatc cctgggcgat
    27181 ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag agcaatacct gatgctggta
    27241 gctgcgacgg aggacttttt taacgcgccc gacaacgccc gccgcgctat tgtgttgtgt
    27301 catctgcagg aacagcggat cgcccattac cgggcggttg agcagccagg aagaactgac
    27361 cggatagcgg ctcatcacgt ccacttcggt atgtggattg aggatgttga tggcatcaag
    27421 taagccgcgc aagatggcgc tgtcgccacg attgccgcaa gtgtggttgc ccagaataag
    27481 taatttcatt ttttcctcat aaatttgatg ccaggtgagg ctgtgtttgt tgtgttcgtt
    27541 gccgtttttt gcctgatgcg acgctggcgc gtcttatcag gcctacaggt cccgagcaca
    27601 gaaccgtagg acggataagg cgttttacgc cgcatccggc aaccgttgtc ggaaccgaaa
    27661 acagcaattc accccgcccg taaaagcatt ttcatttttt cgctgcgaca aaactgacgc
    27721 ttcacttcca ccaccagcgg atggcgtgac agcacaatca tcaccacaaa cgccagcacg
    27781 cccgcggcta tttgcaccgc cagcagcatc cccagcgcca gttgcccttt cagcacaatg
    27841 cccagcgcat aactgaccac cagcgtcggc aacgagagat aaaacggcag ccacaaactc
    27901 aggatgtact ggcgataact ggaaccaaga accggtttaa tcatcacgaa gtaactcaga
    27961 atggtgttga taatctgcac cagcaggaaa ccgagcgtga cgccgattgc gcccgccatc
    28021 tgcccaccaa taacaatcgc cggaataaac agaaatgttt tgaatacatt gaatttaaag
    28081 ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa tcgggttacc cacggagcgc
    28141 agcagaccca caatacacag caattgcagc accggaataa tgctgttcca cttttcgccg
    28201 aataccagcg gtacgaaatt attcgacacc accatcagcc ccagcagcgc cggaaagttg
    28261 ataatcccca ctatcgacag cagcttgtag aagttaacac gcagcttttc ggtgtcgtcc
    28321 tgaattttgg caaacgccgg aaacaacacg cgggtgatga ttgggttcag cttcatcggc
    28381 ggtacgacgg ccacgttgta cgccaggttg tatccccctg ccacgcccgc gccaagaata
    28441 cgcgccagca ccagcgttga aaggttggtg ttgagatagt tgatgatgct gtccgccgtc
    28501 agccaggcac caaaacgtaa gttcggtgcc acagacgcca gcgagaagtg cagaccgggg
    28561 caataaattt tgcgtccaaa gtagccaaac agcagtgttc tcaccgcact attgaccaga
    28621 taaccgagga tcgcggtcat cgccagcggc cagaaatggg cgctaactac cgtaaaagtg
    28681 aagcccgcca gcaccgcgct gg
//
LOCUS       DAJTYI010000040        27177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYI010000040 DAJTYI010000000
VERSION     DAJTYI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..427
                     /locus_tag="OO564_001712"
     CDS             <1..427
                     /locus_tag="OO564_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6652864.1"
                     /translation="KMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQVELA
                     KSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWFTVV
                     ASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(489..1109)
                     /gene="rhtB"
                     /locus_tag="OO564_001713"
     CDS             complement(489..1109)
                     /gene="rhtB"
                     /locus_tag="OO564_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6652865.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1220..2242
                     /gene="pldB"
                     /locus_tag="OO564_001714"
     CDS             1220..2242
                     /gene="pldB"
                     /locus_tag="OO564_001714"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6652866.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2250..3050
                     /gene="yigL"
                     /locus_tag="OO564_001715"
     CDS             2250..3050
                     /gene="yigL"
                     /locus_tag="OO564_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6652867.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3126..4025
                     /gene="bioP"
                     /locus_tag="OO564_001716"
     CDS             3126..4025
                     /gene="bioP"
                     /locus_tag="OO564_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6652868.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3913..4866)
                     /gene="metR"
                     /locus_tag="OO564_001717"
     CDS             complement(3913..4866)
                     /gene="metR"
                     /locus_tag="OO564_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6652869.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5102..7363
                     /gene="metE"
                     /locus_tag="OO564_001718"
     CDS             5102..7363
                     /gene="metE"
                     /locus_tag="OO564_001718"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6652870.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7402..8217)
                     /locus_tag="OO564_001719"
     CDS             complement(7402..8217)
                     /locus_tag="OO564_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6652871.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8479..9240
                     /gene="udp"
                     /locus_tag="OO564_001720"
     CDS             8479..9240
                     /gene="udp"
                     /locus_tag="OO564_001720"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6652872.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9381..10808
                     /gene="rmuC"
                     /locus_tag="OO564_001721"
     CDS             9381..10808
                     /gene="rmuC"
                     /locus_tag="OO564_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6652873.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10903..11658
                     /gene="ubiE"
                     /locus_tag="OO564_001722"
     CDS             10903..11658
                     /gene="ubiE"
                     /locus_tag="OO564_001722"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6652874.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11672..12277
                     /gene="ubiJ"
                     /locus_tag="OO564_001723"
     CDS             11672..12277
                     /gene="ubiJ"
                     /locus_tag="OO564_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6652875.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12274..13914
                     /gene="ubiB"
                     /locus_tag="OO564_001724"
     CDS             12274..13914
                     /gene="ubiB"
                     /locus_tag="OO564_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6652876.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            13993..14262
                     /gene="tatA"
                     /locus_tag="OO564_001725"
     CDS             13993..14262
                     /gene="tatA"
                     /locus_tag="OO564_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6652877.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14266..14781
                     /gene="tatB"
                     /locus_tag="OO564_001726"
     CDS             14266..14781
                     /gene="tatB"
                     /locus_tag="OO564_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6652878.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14784..15560
                     /gene="tatC"
                     /locus_tag="OO564_001727"
     CDS             14784..15560
                     /gene="tatC"
                     /locus_tag="OO564_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6652879.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15602..16384
                     /gene="tatD"
                     /locus_tag="OO564_001728"
     CDS             15602..16384
                     /gene="tatD"
                     /locus_tag="OO564_001728"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6652880.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16381..16869)
                     /gene="rfaH"
                     /locus_tag="OO564_001729"
     CDS             complement(16381..16869)
                     /gene="rfaH"
                     /locus_tag="OO564_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6652881.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17036..18529
                     /gene="ubiD"
                     /locus_tag="OO564_001730"
     CDS             17036..18529
                     /gene="ubiD"
                     /locus_tag="OO564_001730"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6652882.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18575..19276
                     /gene="fre"
                     /locus_tag="OO564_001731"
     CDS             18575..19276
                     /gene="fre"
                     /locus_tag="OO564_001731"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6652883.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19369..20532)
                     /gene="fadA"
                     /locus_tag="OO564_001732"
     CDS             complement(19369..20532)
                     /gene="fadA"
                     /locus_tag="OO564_001732"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6652884.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20542..22731)
                     /gene="fadB"
                     /locus_tag="OO564_001733"
     CDS             complement(20542..22731)
                     /gene="fadB"
                     /locus_tag="OO564_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6652885.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22921..24252
                     /gene="pepQ"
                     /locus_tag="OO564_001734"
     CDS             22921..24252
                     /gene="pepQ"
                     /locus_tag="OO564_001734"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6652886.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24252..24866
                     /locus_tag="OO564_001735"
     CDS             24252..24866
                     /locus_tag="OO564_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6652887.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24905..26356
                     /gene="trkH"
                     /locus_tag="OO564_001736"
     CDS             24905..26356
                     /gene="trkH"
                     /locus_tag="OO564_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6652888.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26368..26913
                     /gene="hemG"
                     /locus_tag="OO564_001737"
     CDS             26368..26913
                     /gene="hemG"
                     /locus_tag="OO564_001737"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6652889.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6244 a         6919 c         7481 g         6533 t
ORIGIN      
        1 aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc tctgctggat
       61 gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc ctgcaccaca
      121 ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca atctcgccaa
      181 tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg ataacgttgg
      241 cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg cgtggtttac
      301 cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac gtctggcgaa
      361 gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt tgattatttc
      421 gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc cgcatccgac
      481 attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat aaacaacgag
      541 ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag agcaatcctt
      601 tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt ggtgacgccg
      661 agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg aaatagcgcc
      721 gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg ctggaacaaa
      781 tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc ggcgcgccac
      841 tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac ttcaaacgca
      901 atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat atgaatcgcc
      961 agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg gttgagcgag
     1021 gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat cgatgtcagc
     1081 aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc
     1141 acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat tttacgtggt
     1201 ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa gagaaaacgc
     1261 gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg atgaagcaga
     1321 gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac agcaccatga
     1381 ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg cggaactggc
     1441 ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg ggcagggacg
     1501 ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta atgattatgt
     1561 tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc gtaaacgcta
     1621 tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac gccatccagg
     1681 tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc gtatgccgtc
     1741 atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc gtgatggcta
     1801 tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac tgacccacag
     1861 cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga ttcgcgtcgg
     1921 tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg tgttagccgg
     1981 tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac gcgtggtgga
     2041 taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc ctgtcgaagg
     2101 aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa aggacgcaat
     2161 gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact cacccagcgg
     2221 aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct gatttagatg
     2281 gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact ctgaagctgc
     2341 tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt gatgtggggc
     2401 aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt gcgcgcgttc
     2461 acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt gccagcgatc
     2521 tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc gacgacgaat
     2581 ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg gtgttcaaat
     2641 atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg tttttcacct
     2701 gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc tggggcgatc
     2761 gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc ggcgtgtcga
     2821 aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag gattgtattg
     2881 cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa ggctgcatta
     2941 tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt attggtacta
     3001 atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa tcgttcttta
     3061 tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc aaagagtttt
     3121 ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt agcttttatg
     3181 gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc gttggcttgg
     3241 cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa acggtcggcc
     3301 tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt ttccgcgctt
     3361 atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg ctctacatca
     3421 cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct tttagcgcct
     3481 tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac catttctgga
     3541 ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg gtgggttaca
     3601 aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg ttctatcttg
     3661 gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag aaaatgccgc
     3721 aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc gggattggct
     3781 actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc attatgaata
     3841 atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa cagccgcact
     3901 ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg catcgtaagt
     3961 gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc gcgctgagcg
     4021 aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg cgtacagtcg
     4081 gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct caaaactctc
     4141 tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt gaatcaataa
     4201 taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa gaaaatgccg
     4261 ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc tggcgagatc
     4321 ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca ccaggcgtac
     4381 ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat cggaggtcat
     4441 caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca cgcccgattt
     4501 aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg gtgtcagcca
     4561 ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg gttcattgca
     4621 ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca acaggatttc
     4681 tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga agccaaggcg
     4741 ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca acgttgccgc
     4801 agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt gttttacttc
     4861 gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata tacagtaacc
     4921 gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt aaatttatgg
     4981 tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg gcgatgaata
     5041 aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta gaggaagaaa
     5101 aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc gcgagctgaa
     5161 aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc tgacagtagg
     5221 gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg acctgctgcc
     5281 ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc tgggtaatgt
     5341 tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt tccgtattgg
     5401 tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca aatggtttaa
     5461 caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca aactgacctg
     5521 gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg ttaagcctgt
     5581 gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac agtttgaccg
     5641 ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag aactggcgaa
     5701 acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac taccacaggc
     5761 gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga aactgctgct
     5821 gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc tgcctgttca
     5881 ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc acaagcgcct
     5941 gccttctgac tggctgctgt ctgcgggtct aatcaatggt cgtaacgtct ggcgcgccga
     6001 tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt tgtgggtggc
     6061 atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc gtcttgatgc
     6121 agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg cactgctgcg
     6181 cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc caattcaggc
     6241 gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg cggcgatcac
     6301 cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc agcgtgcgcg
     6361 ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa ccacggaaat
     6421 tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact accgcacggg
     6481 cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac tggatgtgct
     6541 ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc acctcgacgg
     6601 atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg tgaagccacc
     6661 gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg cgaagtatgc
     6721 gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga ccatactctg
     6781 ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga ttgcgctggc
     6841 gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc agattgacga
     6901 accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt atctccagtg
     6961 gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac aaatccacac
     7021 tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc tggacgcaga
     7081 cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt ttgaagagtt
     7141 tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa acgtaccgag
     7201 cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg cagagcgcct
     7261 gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc gcgcggcact
     7321 ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa ccgggtggta
     7381 ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct taaaccattc
     7441 cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc gataatcagc
     7501 gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat tagcagcccg
     7561 cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct gaccaccata
     7621 taagccgaga accggtgcgt taagatcggt tgcgatatca acaggttgtt tcggtgaatt
     7681 cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta gctgtggatt
     7741 atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga tcattaaacg
     7801 atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat cggccagcac
     7861 ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat cggcaaaatc
     7921 attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc cctccagcgc
     7981 cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct gcactacaat
     8041 gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag gcatgttatc
     8101 cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg tctgaacgat
     8161 ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg ttgccatggt
     8221 attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca tgcaacaaat
     8281 cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag tgatttatgt
     8341 gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg gtgatgagta
     8401 tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg actaaaccga
     8461 ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct cactaaaaac
     8521 gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt ggaaaagatc
     8581 gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac tacctggcgt
     8641 gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg cccgtctacc
     8701 tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg tatcggtaca
     8761 acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac ggcgtctgtc
     8821 cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt cgctgatttc
     8881 gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac tcacgttggc
     8941 gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac ttactctggt
     9001 cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg cgtaatgaac
     9061 tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct gcgtgccggt
     9121 atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc tgagacgatg
     9181 aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg tctgctgtaa
     9241 ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc gtagcgcctc
     9301 gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg tgcgggtaag
     9361 ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt attgcgttgg
     9421 tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa aaagccgagc
     9481 aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa caaattaccc
     9541 aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc agcctgcaaa
     9601 gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg gaagccgcac
     9661 agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc ctcagtgagc
     9721 agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt gatgagcaaa
     9781 accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac ggtttctgcc
     9841 gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg acccacgaaa
     9901 ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac ctgacgcgcg
     9961 cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg acgcgggtgc
    10021 tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc atcgaaaatg
    10081 acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga aaagatgtgg
    10141 tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc gaagacgact
    10201 acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat atccgtttgc
    10261 tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac gtgctgatgt
    10321 ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag ctgatcaccg
    10381 aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg gcgttgcgta
    10441 ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa atcgccgatc
    10501 gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc gcgattggtc
    10561 aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc tcttcaggtc
    10621 gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa attaaacgcg
    10681 agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat cgacttcggt
    10741 cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat aatcagcagt
    10801 cgtgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg acgcccaatc
    10861 tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga taagtcacaa
    10921 gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga tatggtcgcc
    10981 cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat gtcatttggt
    11041 attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg tgggcagacc
    11101 gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg cctggtcgga
    11161 gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat gggacgcgag
    11221 aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa cgctgaggcg
    11281 ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct gcgtaacgtc
    11341 accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg cggccgcctg
    11401 ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta tgatgcatac
    11461 tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga cagctaccgt
    11521 tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc catgatgcag
    11581 gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt ggcgctgcat
    11641 cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag tgacggcagg
    11701 aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa cagcccgctc
    11761 gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt cattgattct
    11821 ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg ctgactgcac
    11881 cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc ttaccgcact
    11941 gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa acttcgttgc
    12001 gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata ccggtgatat
    12061 cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc atcacggcat
    12121 taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta tggcacccgg
    12181 tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg ctgttgatgc
    12241 cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt acggcgccta
    12301 tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc caaaatgcgt
    12361 atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa tcggcataaa
    12421 gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc ggtttggatc
    12481 aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat tgccgatcag
    12541 ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa gcagcagatt
    12601 gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga aatcaagccg
    12661 ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa tggtaaagag
    12721 gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga tctgaaactt
    12781 atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg tctgcgccca
    12841 accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt gctgcgggaa
    12901 tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct ctacatcccg
    12961 gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat ttacggcatt
    13021 ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt gctggcggaa
    13081 cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca tgccgatatg
    13141 caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata tatcggcatt
    13201 gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc agaaaacttt
    13261 atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga ttctggctgg
    13321 gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt ctgtgaacct
    13381 atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa tctgtttaat
    13441 acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca gaaaaccctg
    13501 ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg gaaaacagcg
    13561 aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct ggtgagagca
    13621 tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga actggtttac
    13681 gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc ccgcgagctt
    13741 cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg cgctacgtta
    13801 gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat gcccggctgg
    13861 ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg ctgatttttt
    13921 catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat catctaccac
    13981 agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc cgtcatcgtt
    14041 gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg tgcgtcgatc
    14101 aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac cagtcaggat
    14161 gctgatttta ctgcgaaaac tatctccgat aagcaggcgg atacgaatca ggaacaggct
    14221 aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt tgatatcggt
    14281 tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc gcaacgactg
    14341 cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact ggcgacaacg
    14401 gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag tctgaaaaag
    14461 gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat ggatgaacta
    14521 cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga aaaggcgagc
    14581 gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc gcatgagggc
    14641 gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc agaaaccacg
    14701 ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc accttcccct
    14761 tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt atcacgcatc
    14821 tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg atattcctgt
    14881 gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg atcaagcagt
    14941 tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt acgccgatca
    15001 agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat caggtgtggg
    15061 catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg ctgctggttt
    15121 ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc tttccgctgg
    15181 catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc gacatcgcca
    15241 gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt gaagtgccgg
    15301 tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta cgcaaaaaac
    15361 gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg ccggatgtct
    15421 tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt gtcttcttct
    15481 cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct gaagcagaaa
    15541 gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata tggagtacag
    15601 gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc gtgatgatgt
    15661 tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca ctaacctgcg
    15721 tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt caacggcggg
    15781 cgttcatcct cacgacagca gccagtggca agctgcgact gaagaagcga ttattgagct
    15841 ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta accgcaactt
    15901 ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg ccgcagaatt
    15961 aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga cattgctgga
    16021 gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca cacgcgaaga
    16081 gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg tttgcgatga
    16141 acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga aattgctgat
    16201 cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat catcccggcg
    16261 caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc gtggagaaga
    16321 tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg ggattgcgtt
    16381 ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat taataagatt
    16441 aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct gaaagccttc
    16501 gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg gatcgacaat
    16561 gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta tcgctggcga
    16621 cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg tggtatgaat
    16681 cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac tgactgcggt
    16741 acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat taaccgcctg
    16801 tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca gtaaatacca
    16861 ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa agctttacgc
    16921 taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta atttaacaaa
    16981 tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg ctacaatgga
    17041 cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc agggtgagct
    17101 aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg ctgaccgcac
    17161 tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact caatgccggt
    17221 gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc aggaagatgt
    17281 ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg agccgccaaa
    17341 aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa acatgccgac
    17401 aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg acgtcgatct
    17461 caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga ttacctgggg
    17521 gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt atcgccagca
    17581 gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg gcgcgctgga
    17641 ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg tggcgctggg
    17701 tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc tttcagagta
    17761 tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta tttccaatga
    17821 cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc ctggtgagat
    17881 ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg atagtttccc
    17941 ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt ccacctatac
    18001 cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag tgtttgtacc
    18061 gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg aaggctgttc
    18121 ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga agcgcgtcat
    18181 gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga tcgtttgcga
    18241 tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca cccgtatgga
    18301 cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg attttgcctc
    18361 gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat ggccggggga
    18421 aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca cgtatattga
    18481 cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat gcgcgtttgt
    18541 tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct gtaaagtgac
    18601 ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag acgcggcctt
    18661 ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca aacgtccgtt
    18721 ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg gcgcttctga
    18781 aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc aaatcgtggt
    18841 cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga tgattttgat
    18901 tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt tggcgcgtaa
    18961 cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc tgtatgatct
    19021 ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc cggtggttga
    19081 acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg tattgcagga
    19141 tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga tggcgaaaat
    19201 tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt ttggcgatgc
    19261 gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag aacggcaact
    19321 aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt aaacccgctc
    19381 aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac caaactgaac
    19441 gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg aacaacccag
    19501 cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa tctgctccat
    19561 tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt tcatctcaaa
    19621 cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg aggccggaac
    19681 cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac gcacacgagc
    19741 gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca tggcagctgc
    19801 gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg gatcaaacgc
    19861 cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt cgtcgtaatt
    19921 aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt catttttaaa
    19981 tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa aggcatcctg
    20041 catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc ccatcatgcc
    20101 cgccgctttg gctacattgc ggctcaggcc ggggtgaaaa tcgacgccgt gactcatcgg
    20161 cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc cagtcatgat
    20221 cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat tgacggtaac
    20281 cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga tgttaaaacc
    20341 ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg ccgcttccag
    20401 cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt ccgcacgcac
    20461 gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat cgacaatgac
    20521 aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac gggctggctc
    20581 aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt ccggcacttc
    20641 atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg cgctaccgag
    20701 ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc cgtagaccag
    20761 cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc gcaccacttc
    20821 gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat cgcgcttcgg
    20881 ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct tcggcttacc
    20941 tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa agcggttggc
    21001 atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg ggaagcctgc
    21061 tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca gcagatatgc
    21121 cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc ggaaatccgc
    21181 gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc ggttaacaaa
    21241 gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg cccaggcgac
    21301 aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta ccaacggcat
    21361 tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt ccagcgcgtt
    21421 ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat cctggcgtac
    21481 tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa caaccgcttc
    21541 taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga tcgtggagat
    21601 cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt tcagcagttt
    21661 cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca tgacaaccgg
    21721 cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg cgcccagcac
    21781 agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc cttttacata
    21841 ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg ccagcgggac
    21901 aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg cagcttcaat
    21961 ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg cgaccattcc
    22021 tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg gttccagctt
    22081 cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta aaatcgccat
    22141 cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga ttttcagcgc
    22201 ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt cagcgcccag
    22261 catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt tggtttccgg
    22321 cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca cgcattcgca
    22381 gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca gatcttccag
    22441 gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt caggaacgag
    22501 gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt tgttcgaacg
    22561 cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct cgccgaggct
    22621 ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata ccagttcggc
    22681 aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca tgtcagtctc
    22741 ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt ttgtgttaaa
    22801 aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag aaatcacgct
    22861 ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa
    22921 atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga acggactcgc
    22981 gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga gctgttcaac
    23041 gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg
    23101 ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa gccgaaactg
    23161 tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac ctccttctgg
    23221 actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag cctgctgcct
    23281 gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct gcaactgggt
    23341 attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta ttaccgctcc
    23401 ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc ggtcaatggt
    23461 catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat caatattgcc
    23521 tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat tgtggcgctt
    23581 aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc ggaagagatg
    23641 cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga cctgacccgt
    23701 acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt aaatgatgaa
    23761 caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta ccacatccag
    23821 ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga tatgagtgaa
    23881 gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat tggccatccg
    23941 ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg tacacacctc
    24001 gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc gggcatggtg
    24061 ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg gcgtgaaggg
    24121 cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt
    24181 cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac ccgggatctg
    24241 aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc gttgaagaga
    24301 tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag gcggcgaaag
    24361 cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc gtggcgtggg
    24421 tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg gagccggcgg
    24481 gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg gaaattaccg
    24541 ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta gtgaaagcgt
    24601 atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag acgccattaa
    24661 ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg ttgctggggc
    24721 agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg cgggtggcgc
    24781 ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc cgtggttcat
    24841 tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta aggaagcggc
    24901 agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct tattttcagg
    24961 gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg gccgcgcttt
    25021 tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc cgaaccgcaa
    25081 agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt tttggaccgt
    25141 gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc tcacgattac
    25201 cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta cgctggtggg
    25261 gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat ggtttggcgg
    25321 gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg gtgggatgca
    25381 gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc
    25441 ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct gtgcgctggc
    25501 gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg cgactatcgc
    25561 tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc cggctattaa
    25621 cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc acttttcact
    25681 gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca tgtttatcgg
    25741 cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata atgtctacag
    25801 ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg cgacaaccgc
    25861 cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac tgcttttatg
    25921 ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag tgatccgcat
    25981 cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc cgaacgccgt
    26041 ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag ccgtttgggg
    26101 atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta tcgccacggg
    26161 cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc tggggccagg
    26221 gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat ggatcctgat
    26281 tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct ttaccccgac
    26341 tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc aacaagggac
    26401 ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact ggggatccag
    26461 acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta tgaccgtgtg
    26521 gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga atttgtcaaa
    26581 aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa cctggtggcg
    26641 cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt tctgatgaac
    26701 tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta cccacgttat
    26761 cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg tgaaacggat
    26821 acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc
    26881 gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa tgcgcggtca
    26941 gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac
    27001 tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaattccgac
    27061 agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg cacaccccgc
    27121 gccgctgaga aaaagcgaag cggcactgct ctttaacaat ttatcagaca atctgtg
//
LOCUS       DAJTYI010000041        27173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYI010000041 DAJTYI010000000
VERSION     DAJTYI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO564_001738"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO564_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6652890.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO564_001739"
     CDS             complement(1614..1937)
                     /locus_tag="OO564_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6652891.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO564_001740"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO564_001740"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6652892.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO564_001741"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO564_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6652893.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO564_001742"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO564_001742"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6652894.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO564_001743"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO564_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6652895.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO564_001744"
     CDS             7537..8574
                     /locus_tag="OO564_001744"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6652896.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO564_001745"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO564_001745"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6652897.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO564_001746"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO564_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6652898.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO564_001747"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO564_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6652899.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO564_001748"
     CDS             10745..11626
                     /locus_tag="OO564_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6652900.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO564_001749"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO564_001749"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6652901.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO564_001750"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO564_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6652902.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO564_001751"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO564_001751"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR6652903.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO564_001752"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO564_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6652904.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO564_001753"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO564_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6652905.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO564_001754"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO564_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6652906.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO564_001755"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO564_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6652907.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO564_001756"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO564_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6652908.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO564_001757"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO564_001757"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6652909.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO564_001758"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO564_001758"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6652910.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO564_001759"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO564_001759"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6652911.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO564_001760"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO564_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6652912.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO564_001761"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO564_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6652913.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO564_001762"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO564_001762"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6652914.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO564_001763"
     CDS             24893..25273
                     /locus_tag="OO564_001763"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6652915.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO564_001764"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO564_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6652916.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO564_001765"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO564_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6652917.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27173
                     /locus_tag="OO564_001766"
     CDS             26442..>27173
                     /locus_tag="OO564_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6652918.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLW"
BASE COUNT         6702 a         6770 c         7124 g         6577 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgctg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tgg
//
LOCUS       DAJTYI010000042        27137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYI010000042 DAJTYI010000000
VERSION     DAJTYI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            111..764
                     /gene="ribB"
                     /locus_tag="OO564_001767"
     CDS             111..764
                     /gene="ribB"
                     /locus_tag="OO564_001767"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6652919.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1025..1195
                     /locus_tag="OO564_001768"
     CDS             1025..1195
                     /locus_tag="OO564_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6652920.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1253..2026)
                     /gene="zupT"
                     /locus_tag="OO564_001769"
     CDS             complement(1253..2026)
                     /gene="zupT"
                     /locus_tag="OO564_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6652921.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2169..2957
                     /gene="ygiD"
                     /locus_tag="OO564_001770"
     CDS             2169..2957
                     /gene="ygiD"
                     /locus_tag="OO564_001770"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6652922.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(2995..4155)
                     /locus_tag="OO564_001771"
     CDS             complement(2995..4155)
                     /locus_tag="OO564_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6652923.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4161..4832)
                     /locus_tag="OO564_001772"
     CDS             complement(4161..4832)
                     /locus_tag="OO564_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6652924.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(4980..6461)
                     /gene="tolC"
                     /locus_tag="OO564_001773"
     CDS             complement(4980..6461)
                     /gene="tolC"
                     /locus_tag="OO564_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6652925.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            6666..7295
                     /gene="nudF"
                     /locus_tag="OO564_001774"
     CDS             6666..7295
                     /gene="nudF"
                     /locus_tag="OO564_001774"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6652926.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7296..7718
                     /locus_tag="OO564_001775"
                     /pseudo
     CDS             7296..7718
                     /locus_tag="OO564_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            7743..8570
                     /gene="cpdA"
                     /locus_tag="OO564_001776"
     CDS             7743..8570
                     /gene="cpdA"
                     /locus_tag="OO564_001776"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6652927.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            8570..9151
                     /gene="yqiA"
                     /locus_tag="OO564_001777"
     CDS             8570..9151
                     /gene="yqiA"
                     /locus_tag="OO564_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6652928.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9180..11072
                     /gene="parE"
                     /locus_tag="OO564_001778"
     CDS             9180..11072
                     /gene="parE"
                     /locus_tag="OO564_001778"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6652929.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11120..11434)
                     /locus_tag="OO564_001779"
     CDS             complement(11120..11434)
                     /locus_tag="OO564_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6652930.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11465..12046)
                     /gene="mdaB"
                     /locus_tag="OO564_001780"
     CDS             complement(11465..12046)
                     /gene="mdaB"
                     /locus_tag="OO564_001780"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6652931.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            12365..12697
                     /locus_tag="OO564_001781"
     CDS             12365..12697
                     /locus_tag="OO564_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6652932.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(12743..14092)
                     /gene="qseC"
                     /locus_tag="OO564_001782"
     CDS             complement(12743..14092)
                     /gene="qseC"
                     /locus_tag="OO564_001782"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6652933.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14089..14748)
                     /gene="qseB"
                     /locus_tag="OO564_001783"
     CDS             complement(14089..14748)
                     /gene="qseB"
                     /locus_tag="OO564_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6652934.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14900..15292
                     /gene="ygiW"
                     /locus_tag="OO564_001784"
     CDS             14900..15292
                     /gene="ygiW"
                     /locus_tag="OO564_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6652935.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15345..15827
                     /locus_tag="OO564_001785"
     CDS             15345..15827
                     /locus_tag="OO564_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6652936.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            16373..18631
                     /gene="parC"
                     /locus_tag="OO564_001786"
     CDS             16373..18631
                     /gene="parC"
                     /locus_tag="OO564_001786"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6652937.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            18865..19602
                     /gene="plsC"
                     /locus_tag="OO564_001787"
                     /gene_synonym="parF"
     CDS             18865..19602
                     /gene="plsC"
                     /locus_tag="OO564_001787"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6652938.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            19677..21089
                     /gene="ftsP"
                     /locus_tag="OO564_001788"
     CDS             19677..21089
                     /gene="ftsP"
                     /locus_tag="OO564_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6652939.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21200..23417
                     /locus_tag="OO564_001789"
                     /pseudo
     CDS             21200..23417
                     /locus_tag="OO564_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(23460..23717)
                     /gene="yqhH"
                     /locus_tag="OO564_001790"
     CDS             complement(23460..23717)
                     /gene="yqhH"
                     /locus_tag="OO564_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6652940.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(23768..24694)
                     /locus_tag="OO564_001791"
     CDS             complement(23768..24694)
                     /locus_tag="OO564_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6652941.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(24894..25721)
                     /gene="dkgA"
                     /locus_tag="OO564_001792"
     CDS             complement(24894..25721)
                     /gene="dkgA"
                     /locus_tag="OO564_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6652942.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(25826..26989)
                     /gene="yqhD"
                     /locus_tag="OO564_001793"
     CDS             complement(25826..26989)
                     /gene="yqhD"
                     /locus_tag="OO564_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6652943.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
BASE COUNT         6340 a         6768 c         7220 g         6809 t
ORIGIN      
        1 agtaacgatt ctgtcgggca tggacccgct cacgttattt tggctatatg ccgccactcc
       61 taagactgcc ctgattctgg taaccataat tttagtgagg tttttttacc atgaatcaga
      121 cgctactttc ctcttttggt acgcctttcg aacgtgttga aaatgcactg gctgcgctgc
      181 gtgaaggacg cggtgtaatg gtgcttgatg atgaagaccg tgaaaacgaa ggcgatatga
      241 tcttcccggc agaaaccatg accgttgagc agatggcgct gaccattcgc cacggtagcg
      301 gtattgtttg cctgtgcatc actgaagatc gccgtaaaca acttgatctg ccaatgatgg
      361 tagaaaataa caccagcgcc tatggcaccg gttttaccgt gaccattgaa gcggctgaag
      421 gtgtgactac cggtgtttct gccgctgatc gtattacgac cgttcgcgca gcgattgccg
      481 atggcgcaaa accttctgat ctgaatcgcc cgggccacgt tttcccactt cgcgcgcagg
      541 caggtggtgt actgacgcgt ggcggtcata ctgaagcgac tattgatttg atgacgctgg
      601 caggctttaa accggctggt gtgctgtgtg agctgactaa tgacgatggc acgatggcgc
      661 gtgcaccaga gtgcattgag tttgccaata aacacaatat ggcgctcgtg actattgaag
      721 acctggtggc ataccgtcag gcacatgagc gtaaagccag ctgaaaaccg ctgcttaatt
      781 actgccttaa tcaagaaacc gaagttgtag ctggcttcgg tttttatttt gccctgctat
      841 gcaagatgtt aattccgctt ctctttctat gagaaaattt cattaatatc aggcattctt
      901 tttcattatg atcaaaatca tataattgtt ccgttattat gcgaccatat gaatatatcc
      961 gaaaaaggat atgtgaaatt tatgcgctaa atgtttagta ttgttaaata ttaagggtaa
     1021 aaggatgttt atcgcctggt actggattgt attgattgct ctggttgtgg tgggttattt
     1081 cctgcatttg aaacgttatt gtcgggcgtt tcgccaggac agagacgcac tgcttgaagc
     1141 gcggaacaaa tacttaaaca gtacgagaga agagacagcc gaaaaggtag aataaggctt
     1201 tcgtgaagct ggcagaagtt gtccatgtgc tggacaactt ctgatgttgt ttttaaccaa
     1261 ttcccgccgt ttgtagcagc actaaactga atcccatcac tgacattcca catagtacgc
     1321 catagctggg gttattatta gggtcaatct ctttggcgag cggcattaat tcatcaaccg
     1381 agagcgccac cataattcct gcaaccgccg ccatgattgc cgccatgacc accggggaaa
     1441 tcatgctacc gaggattaac cacgccagca caccaccaag aatttctgct agtccagaaa
     1501 tccccgccca cagaatcgcg gtgcgtttag accccgttgc cgcataaacc gggcctgcca
     1561 ctgccagacc ttcagggata ttgtgcaacg cgacggccag tgcgatgcca aatcccagct
     1621 ccaggttgct gctcgccgtg acaaaggtgg caatcccttc cgggaagtta tgcagactga
     1681 tgccgagagt gagcagaatg gctgtgcgct tgatcgattt tggcaacagc tgcaccgatt
     1741 tttgcattaa atcctgcgga tgcgcatgcg gcaacatgcg gtccaggcca aagtagccaa
     1801 gcagaccgaa gataaacatt ccataaccca acaccggcga cattccttca gctgctagtg
     1861 cggcaggaag catttccatt aatgagatga gcaacatgat ccccgccgca aaacctagcg
     1921 aaaacgccag taagcggttc gagggttttt gtccgagaac gccgagaaac gcgccaataa
     1981 acgtggctgc ccccgccagt atggtcagaa tgagaggtac tgacatcgac aactccttat
     2041 aaatcttcac cttcaggcag atcgtcaaaa taactgatga tcatcatcat tgttatccgg
     2101 gttcttactc cgccgaacaa gcgtattgtg aggatatcaa aatgacacct ttagttaagg
     2161 atatcatcat gtcttcaaca cgtatgccag cattgttttt aggtcacggt agtccgatga
     2221 acgtgctgga agataatttg tatacccgca gctggcagaa gttggggatg acattgccac
     2281 gcccgcaagc gattgtggtg gtttcggctc actggtttac ccgtggaaca ggagtgaccg
     2341 cgatggagac gccgcccacg attcatgact ttggtggctt cccgcaggcg ctgtacgata
     2401 cgcattatcc tgctccgggt tcgcctgcgc tggcacagcg tctggttgag ctgttagcgc
     2461 cgatcccggt gacgctggat aaagaagcct ggggctttga ccacggctcg tggggcgtgc
     2521 tgattaagat gtatcctgac gccgatatcc ctatggtgca gttgagtatc gacagtagca
     2581 aacctgccgc ctggcatttc gaaatggggc gcaaactcgc ggcgttgcgt gatgaaggaa
     2641 taatgttggt cgccagcggt aacgtggtgc ataacctgcg cacagtgaag tggcacggtg
     2701 atagttcacc gtatccgtgg gcgacgtcgt ttaatgagta tgtgaaagcg aatctgacgt
     2761 ggcaagggcc ggtagagcag catcctctgg tgaattatct cgaccatgaa ggtggcgcgt
     2821 tatcgaaccc aacgccagag cactatctgc cgttgttgta tgtgttaggt gtgtgggatg
     2881 ggcaggagcc aattaccatt ccggtcgatg gtatagaaat gggcagttta agtatgctgt
     2941 cggtgcagat aggctaacaa ttaccggatg gcgagtgcca tccggtatcg tggcttattc
     3001 aacaaaaata tgtggataaa accgagacat atcctgggtg atcaacgcac ggtcttcacg
     3061 aatgccaatt ccggcaggtt gatcgttcac cagccagcta ccaatcagca tatagctgtc
     3121 gccgaatttc ggtaacgggt ggaattgctg aacaatcatc ccttcttcgc catacggacc
     3181 ttccgctgct tcaatggttt tgccgttctc aatgatcgac acgtttgcgc cttcacggga
     3241 gaagatcggt ttaaccacat atttttccat ttgcggatga tcatcttccg caaaataagc
     3301 gggcagcagg ttcgggtgat tcgggaacat ctcccacagt agcggtagaa gtgccttgtt
     3361 ggagataata ctcttccacg ccggttccag ccagcgtacg ccagcatctt ccagcttggt
     3421 ggagaacatt tcgcgcagca taaattccca cggataaagc ttgaacaggt tagaaatcac
     3481 ctgatcctgt aaatccgtga actggccttt ttcgcctaaa cctatctcgt cgatgtagag
     3541 gaactcggtg gcgatttcag cttcggttgc gcagtcctgc aaatactgaa tggttccgcg
     3601 atcttccacc gtgtcgcgac agcaggtgag atgcagcaac tggaagccac actgttcacg
     3661 cagctcaacg aagcgatcga tcagtttttc ttgcagactg ttaaactggt cgctgccctc
     3721 cggcaagtta ccggcgttaa gctgatcttc cagccagatc cactgaaaga acgccgcctc
     3781 gtatagtgac gttggcgtat cggcgttatt ttccagaagt ttaggttcgc cagtgccatc
     3841 ccacgccaga tcaagacgcg aataaagcga tggctggtga gtcagccatg attgacgcac
     3901 aaaactccag gtgtgttttg gtatgcggaa tttggtcatc agctcatcgc tggcgatcac
     3961 tttttccacc actttcaggc acatctggtg cagttcggcg gtgacttctt ccagcttttc
     4021 aacctgggcg agggtcaact tgtagtaagc atcttcacac cagtacggct cgccgtacat
     4081 ggtgtgaaaa ttgaaaccgt attcgtgggc tttctcacgc cagtccgggc gctcggtaat
     4141 actgactctt tccatcggta tcagccaccc attgaacgag aagaggtgcc ggttgcgcta
     4201 cgctgcatag tgctttgttt ggcaacagat tcaccaaaac cgccacgggt aacggtagtg
     4261 gtggtcgccg gttttggtgc cattgccgtc ttcggtacgg tcatggtgcg gcctggctgg
     4321 gctgcgccat agtttttacc cgtcgcgtcg gtatatttac cgtaagccgg actggccggg
     4381 tttttcgagg agaacagcgg ctgctgtgca aatcccgcgc cgccgcccat cagacgcccc
     4441 atcatgtaac cggccatcag cggcatccag aaactcccgc tggattgctg agcctgcgcc
     4501 tggttttctg gtgccatgcc agcctgggct ggtgcttgct gacactgacc ttcaccaaat
     4561 tcagcaacac agtcttcacg ggtggcgtat ttcggcgcag tacgttcggc ttctttcagc
     4621 gcattgttgt acgcggtggt acattcggcg cttttgcctg ggtttgcagc tgaacagtcg
     4681 tcagcatttt gatagagaga cactgtttca tcactctttt cacagccagc cagcataaaa
     4741 acagtggcaa ccgcgagagc gactggtgtc agatggcgtg cgctccagtt tttgcggaac
     4801 gatgcgtggc gtatggattt tgtccgtttc atttttgtct tccgggacca gtggtaaata
     4861 cccatcagaa tagaggatgg ctggtcgaaa ttgaagcgag aagggggaag aatgcggcag
     4921 ataacccgta tctttacgtt gccttacgtt cagacggggc cgaagccccg tcgtcgtcat
     4981 cagttacgga aagggttatg accgttactg gtggtagtgc gtgcggatgt ttgctgaacg
     5041 acgggtgccg ggctatcagg cgcataacca tcagcaatag cattctgttc cggcgtttgc
     5101 ggggcaacgt tttccggatt agtggaaacc ggtttgctca gcgcattgtt cagtgccagc
     5161 aaatcctgct cgttcaacgt acccagggct gacttaatat tcagctgatt aatcaggtag
     5221 ttataacgcg cattcgccag ctcttgcttg gcgttgtaca gcgtggtggt cgcatccaac
     5281 acatcaacaa tggtacgcgt accgaccgag tagcccgctt ccatcgcgtc taatgagctt
     5341 tgcgcggaaa ctacggcttg tttgtaggcg ttaatactac tgatagaagc attaatgttg
     5401 ttgaaggagg aacgtacggt ttgcacgacg ctacgatgtg cgctttccag ttgctcactg
     5461 gcaccaacaa agttgtactg tgcctgtttc acctgcgagt taaccattcc gccctgataa
     5521 atcggcagcg agaagctcag gccaactttg ttctggccca tattgctatc gtcatactgg
     5581 gtaccagcgg caccacgggt tttcgaaccg ctataagagg tgtcagaaat cccggtagaa
     5641 gccgttaaat ccagagtcgg taagtgacca tcctgcgcct ggcgaatttg ctcgcgcgcc
     5701 aggtcctggc tcaagcatgc ctgtaacagc gacaggttgc gtttttcggc ttctttcagc
     5761 agcgcgttaa ccggctgtgg tttgtcggtt ttaaagtttt cgacattcag cgccgccagt
     5821 tccggatagt agttaccggt gatctggcgc agctgctcta ccgcgttatc aaggttatta
     5881 cgtgcggtca cttcgttcgc cagcacggta tcgtactgcg cgcgggcgtt ctgcacgtcg
     5941 gtgatcgcta ccaggcccac gttaaaacgt tgggtggttt gatctaattg acggtagatc
     6001 gcttcttttt gtgcctgtgt ataggaaaga acgtcaatag cattcaacac gttgaaataa
     6061 gcggtcgcgg tgttgaggat caaggtttgc tgatcggtct gatatgtgac gtcctgaatc
     6121 cctgctgctt tttcctgcag cgttaacgca cgccatttcg acatatcaaa aatggattga
     6181 gttaactgca gggacgcact ggtcgcgttc gagttgatgc cgttcgcgtc gcggtagccg
     6241 ttgctatagg tgtaatctgc acctaaacct agctgtggca gtaatggact gcgcgcttca
     6301 ttaatttttt caaaggcagc atcacgatcg gcggcagact tacgcaattc cgggttacta
     6361 aggcgtgctt gctgataaac ttgcatcagg ttctcggcct ggctcaacga actgaaccca
     6421 gaaaggctca ggccgataag aatggggagc aatttcttca tttgcattcc ttgtggtgaa
     6481 gcagtattta gcgcgatcaa actgtaaaat tattgcggat tctagcagaa gccgctaccg
     6541 caaaaggttg gcgttacgtg ccatagggcg ttaatgtgcc tcaatttaac atatgactgc
     6601 tcaaacggca gtcaaacgtc gctgaaattc acatttaatt cactattagt gccaggacat
     6661 taacaatgct taagccagac aacctgcccg ttacatttgg caaaaacgat gtagaaatta
     6721 ttgcacgaga aacactttat cgcggctttt tttcattaga tctttataga tttcgtcatc
     6781 gtctattcaa cgggcaaatg agtcatgagg tacggcggga aatttttgag cgcggtcacg
     6841 ccgcagtctt gctacccttt gacccagtgc gtgatgaagt tgtgctgatt gagcagattc
     6901 ggattgccgc gtacgacacc agcgaaaccc cctggctact ggagatggtt gccgggatga
     6961 ttgaagaggg tgaaagtgtg gaagatgttg cccgtcgcga agcgattgaa gaggcgggac
     7021 tgatagtcaa acggaccaaa ccggtgttaa gtttcctggc aagcccgggg ggcaccagtg
     7081 agcgttcgtc aattatggtg ggcgaagtgg acgccacgac cgcaagcggt attcatggtc
     7141 tggctgatga aaacgaagat attcgcgttc atgtggtaag ccgggaacag gcataccagt
     7201 gggtagaaga ggggaaaatc gacaacgcag cgtcggtcat cgctttgcaa tggctgcagc
     7261 tgcatcatca agcgttaaaa aatgagtggg cataaatgaa gcgttacaca cctgactttc
     7321 ctgaaatgat gcgcctgtgc gagatgaact tttcataatt gcgccgtttg ttaccgcgca
     7381 atgacgcacc cggcgaaact gtaagctatc aggtggcaaa cgcacaatat cggctgacga
     7441 ttgtggaatc gacccgatac actaccctgg tgacaataga acagactgcg cccgcgatca
     7501 gttactggag ccttccgtca atgacggtgc gtctgtatca tgacgcgatg gtggctgaag
     7561 tgtgttcaag ccagcagatt tttcgcttca aagcgcggta tgattatcct aataaaaagt
     7621 tgcatcaacg cgacgaaaag catcaaatta accagttttt agcggactgg ttgcgatact
     7681 gtttagcaca tggagcgatg gcgattccgg tttactagcg tcgtgaaacc taaggacacc
     7741 atttggaaag cctgttaacc cttcctctgg ctggtgaggc cagaggaagg attttacaaa
     7801 ttaccgatac tcacctgttt gcacaaaagc acgaagccct gttaggggta aacacctggg
     7861 aaagctacca ggcggtgctg gaggcgattc gtccacacca gcacgaattc gacctgattg
     7921 tcgcgacagg tgatttagcg caggatcaat cctctgcggc ctatcagcat ttcgctgaag
     7981 gcatcgcaag ttttcgtgcg ccctgcgtct ggctgccggg caaccacgat ttccagcccg
     8041 cgatgtacag cgcgttacag gatgcgggta tctccccggc gaagcgggtg tttattggtg
     8101 agcaatggca aatcctgttg ctggatagcc aggtgtttgg cgtgccgcac ggtgagctga
     8161 gcgagtttca gcttgagtgg ctggaacgta aactggccga tgcgccagaa cgccatacgt
     8221 tgctgctgct gcatcatcat ccgctacctg cgggttgtag ttggctcgat caacacagtc
     8281 tgcgtaacgc gggcgaactg gataccgtgc tggcgaagtt tccgcacgtc aaatacttgc
     8341 tgtgcggtca tattcatcag gagctggatc tcgactggaa cggtcgccgc ctgctggcaa
     8401 cgccgtcgac ctgtgtgcag tttaagccgc actgttccaa ctttacgctg gatactatcg
     8461 cgcccggctg gcgtactctc gagttacatg ctgatggcac gctgaccacc gaggtgcatc
     8521 gcctagcgga cacacgtttc caacctgata ccgcttcaga aggctactga tgtctacgct
     8581 tctttattta cacggtttca acagctcgcc gcgctctgca aaagcgagct tgttaaaaaa
     8641 ctggctggcg gaacatcacc ctgacgttga gatgatcatt ccgcagttgc cgccgtatcc
     8701 ttccgacgcg gcagaactgc tggaatccat tgtcctggaa catggcggcg attcgctggg
     8761 tattgtcggt tcgtcactgg ggggatatta cgccacctgg ttgtcgcaat gttttatgct
     8821 gcccgcagtg gtggtaaacc cggcggtacg cccgtttgaa ttgctgacgg actatctcgg
     8881 tcagaacgag aacccctaca ccgggcagca atatgtgcta gagtcacgcc atatttacga
     8941 tctcaaagtc atgcagattg acccgctgga agcgccggat ttgatctggc tgctgcaaca
     9001 gacgggagat gaagtgctgg attaccgcca ggcggtggcg tactacgctt cctgccgcca
     9061 gactgtcata gaaggcggca accacgcatt cacgggcttc gaagattatt tcaacccgat
     9121 cgtcgatttt cttggtctgc accatctctg acgatcaact cgaattgcta acttaaacca
     9181 tgacgcaaac ttataacgct gatgccattg aggtactcac cgggcttgag ccggttcgcc
     9241 gccgtccggg gatgtatacc gataccactc gccctaacca tttggggcaa gaagttattg
     9301 ataacagtgt cgatgaagca ctggcgggtc acgcaaaacg cgtggatgta atcttacatg
     9361 ctgaccagtc gttagaagtg attgacgatg ggcgcgggat gccggtggat attcacccgg
     9421 aagaaggagt accggcggtt gaactgattc tttgccgtct gcatgcaggc ggtaaattct
     9481 ctaacaaaaa ttaccagttc tctggcggcc tgcatggcgt ggggatttcg gtagttaacg
     9541 ccttgtcgaa gcgcgtagaa gttaacgtgc gccgcgatgg tcaggtttat aacatcgcct
     9601 ttgaaaatgg cgaaaaggtg caggatttac aggttgtcgg cacttgcggt aaacgcaata
     9661 ccggtaccag cgtgcacttc tggccggatg aaaccttctt tgacagcccg cgtttttctg
     9721 tttcacgcct gacgcatgtg ctgaaggcca aagcagtact gtgccccggc gttgagatta
     9781 cctttaaaga tgagatcaac aacaccgaac agcgctggtg ctatcaggac ggtctgaatg
     9841 attacctggc ggaagcggta aacggtttac cgacgctgcc agaaaaaccg tttatcggta
     9901 atttcgctgg cgatactgaa gctgtggact gggcgctact gtggctgccg gaaggcggtg
     9961 aactgctgac cgaaagctac gtcaacctta tcccaacgat gcagggcggt acccatgtta
    10021 atggtctgcg tcagggcctg ttggacgcga tgcgtgagtt ctgtgaatac cgcaacattc
    10081 tgccgcgcgg cgtgaagctg tcggcggaag atatctggga tcgctgcgcc tatgttctgt
    10141 cggttaaaat gcaggatccg cagtttgccg ggcagaccaa agagcgtctc tcttcgcgtc
    10201 agtgtgcggc attcgtttcg ggcgtggtga aagatgcctt catcctgtgg ctgaaccaga
    10261 acgttcaggc ggcggagttg ctggcagaga tggcgatttc cagcgcccag cgtcgtatgc
    10321 gtgcggctaa aaaagtggtg cgtaaaaagc tgaccagcgg cccggcgctg cctggcaaac
    10381 tggctgattg taccgcgcag gaccttaacc gtaccgaact gttcctcgtg gaaggtgact
    10441 ccgcaggcgg atctgccaag caggcgcgcg atcgtgaata tcaggcgatc atgccgctga
    10501 aaggtaagat ccttaacacc tgggaagttt cttccgacga agtgctggcc tcgcaggaag
    10561 tgcatgatat ttcggtagcg atcggtatcg atcctgacag cgacgatctg agccagcttc
    10621 gttatggcaa aatctgtatc ctcgcggatg cggactctga tggtctgcac attgccacgc
    10681 tgctctgcgc tttgttcgta aaacacttcc gcgcgttggt gaaacacggt cacgtttacg
    10741 tcgcactgcc accgctctac cgtattgatc tcgggaaaga ggtttattac gcgctgacgg
    10801 aagaagagaa agagggcgta cttgagcaat taaaacgcaa gaaaggcaag ccgaacgtcc
    10861 agcgttttaa aggtctgggg gaaatgaacc cgatgcaatt gcgtgaaacc acgcttgatc
    10921 cgaacactcg ccgtctggtg cagttgacta tcgatgatga agacgatcag cgtactgacg
    10981 cgatgatgga tatgctgctg gcgaagaaac gctcggaaga tcgccgcaac tggttgcaag
    11041 agaaaggcga catggcggag attgaggttt aaaggaaaga acattgcccg gtcaggatgg
    11101 ccgggcaaac aaggcaggat taaatccctg gctgcagaat acggatattc atctccagca
    11161 cgtcgccttt tacggctttg ctgtacgcct tcatgtgcgg ggtttgcaga tgcgcttcaa
    11221 ggtgcgcgat gctttcccac tgctcaatca tcacgataga atccggtgcc atagactgga
    11281 aactcacgcc agcagcgcaa tccaccattg gcgcatagcc gtggcaacct tcttctttca
    11341 gtacggttgg aacgatttta gcaaactgat ccaataccgc ctgacggtga tgttgaccag
    11401 gacgagtacg gatttctgcg attacggtaa gcatggttaa ctccttctaa agcctgagct
    11461 ctagttaacc aaaaatttcc acaagatgct tgcgatattc ttcagtatag cggggaacat
    11521 caggcatttt tatcacgtca ttagcgataa atgtcggcag cggttccata ccgaggaatt
    11581 ggtttgcttt atggaacggc agatacacac cgtcaacgcc aacgccgtgg aagaactgat
    11641 ctttttcggt gaaggcttcc attggtgcgt tccaggttag agaaagcata tattttttgc
    11701 cctgtaccag gccgccggaa ccgtattttt tcgacggatc tttgcgggta cgaccatcgc
    11761 tggcatacag agtcccgtga ccttcggtga atacatcatc aatatatttt ttcactgtcc
    11821 acggcgcgcc catccaccag cctggcatct gccagatcac cacatcagcc cagagaaagt
    11881 tttgtacttc cgctttgaca ttgtagtcgc tgtcggcgcg aacgatgcgg acatcatgcc
    11941 cgaggtcgcg cagtgtgcca tccgcgactt cggtcagggt gtcgttcagt tgaccattgg
    12001 agtgggcgaa ttttttcgcg ccgttgataa tcaggatgtt gctcattttt taacctcaag
    12061 gtgagacgtt tgtcgactat agtacgtcac agagcggtac ggtgaaatta gcaaaatgtg
    12121 caaagtcttt tgcgaattta gcaaaagtct gatctagctc tgactcaccg atgcaggata
    12181 aatattttct gaaaatgaca atactgatag ccattcctca taattcttac tcgtaccgga
    12241 ataatttaca cgtttggttc ggtttttttg gggattgaaa atatggcttt gaataagtaa
    12301 aagatgaaaa tatcaacgtg tcatgttgat aattataaat ggatttttat gagcgtactg
    12361 atatatgtta aagcagaaaa ttaaaacaat atttgaagca ttgctatata taatgcttac
    12421 ttattggtta atagattctt tttttgcttt caataagtat gactggatgc ttgagtcggg
    12481 aggcaatatt tgcagtatac cttccgtttc aggcgaagat cgtatattgc aagcaatgat
    12541 tgcagcattt tttcttctta cgccacttat tattcttatc ttaagaaaac tctttatgcg
    12601 ggaaatgttc gagttttggc tatatgtatt ctcgctcgtt atttgtctgg tttgtggttg
    12661 gtggcttttt tggggacggt ttatattttg ctattagtaa aatctatgag agtcgtttta
    12721 acggctctca tagacagaga agttaccagc ttaccttcgc ctcaaatcca ccttgttccg
    12781 cattcccaaa ctcaacattc atgtcatgca gcttggcgat tcgctggaca atcgatagcc
    12841 caagtccact gccggtagcg gtttgtccgg gtgggcgata gaagcgttcg ccaattcgcg
    12901 ccagtgcctc tggtgtcaca ccggggccgt tatccctcac gatgaaatta tcagcattca
    12961 gcgtgacgtc tatcacgctg ccctgtggac tgtagcgcac ggcgttatcc agcaaatttc
    13021 gcaccaacaa acttagcaat agcggttgtc cggtgcgttt gatgccgtgg gcattgagtg
    13081 tcagtcgcac gtcaattttc gcctgctgcg ccgtgtggta aatatccatc accgacgatt
    13141 gtaggagatc ttcaagcggg atctccgcga cgtcctgaag gttatccagt gagtccagcc
    13201 gcgatagcgt gagcagttga tcaaccagac gagtagcgcg atcgatcccg gaatgtaatt
    13261 ggagcagtgc ttttttccgc gcctgcggat catcgtcaga gagctgcgca acttcggttt
    13321 gcactttcag cgctgttaac gggctacgta gttcgtgagc tgcatcggag gtaaagcgtc
    13381 gttctcgaac catcatcgca tgtgtgcggg cgaacagttg atttagcgac tcaaccagtg
    13441 gccgcacttc gctgggtacg ccagtcgcgt ttagtggttt ttccgagtca gggtcacgca
    13501 tacgtagtgc cagcgccagt ttgttcagcg gcgcgagttc acgacccagt agtaccatca
    13561 tgatgattaa cataatcggc agtgcgacca gccacgggat caattgcccg gcaacaatcg
    13621 ccagcgccat gtcttcacgg tattcccatt cctggccaac aacgatgcga tatttgccat
    13681 caggtgaggt catccagaca aaacgccaag gatcgtcttc accgaccagt tgcccgtcag
    13741 caaaaccttc ccgttgatag ctatagggaa tatcttctcc gttatcgcca tcattaagga
    13801 ccattctgcc gtcgtgggta aagatggcaa aggtcagcgc atcgtcatca acatgaccgt
    13861 gttttaattt atttggcgtc tgtgccatgc gatccgccgc gttgatttcg ttgagatcga
    13921 gcgtacttaa ccgtttggca aacagcatca gttgggtgtc gaacaattca tcgacgttat
    13981 ccgttgtttg tttccaggcg acaaagctgg aaagcagcca ggtcaccgag gccagaatta
    14041 aaaagattag cgtcagcctg acgcgcagac taagacgttg ggtaaatttc atttctcacc
    14101 taatgtgtag ccaataccat gcacggtacg aataaaatca ctaccgagtt tgcgtcgcag
    14161 atgatgcaca tgcacttcaa cggcattact ggtgacctct tcgtcccagg tatacagttt
    14221 ctcttcaatc agtttgcgcg gcagtacccg accagcgtta cgcatcagta attccagcag
    14281 ggcaaattct tttggtttca gtgttaaggg ttcgccagcc agcgtggcga tacgtttgcc
    14341 ggggtcgagc atgacgttac cgtggcgcag ctcgttgctg gcctggccgt tggttcggcg
    14401 catcagagcc tccagcctgg cggcgacttc tatcaacgca aaaggtttac acagataatc
    14461 gtcagctccc agacgcagcc cttctacacg ttccgctaac gcatcacgcg cggtcaggtt
    14521 cagtaccggc tcacgctgac ctttctctcg ccattcgcgc aaaatatcgc gaccatccat
    14581 gcctggtaag gttaaatcca ggatcaccgc atcataaggt gcgctataaa gcgcctcttt
    14641 tccctgacga ccttgtgtaa accagtcgac actaaaaccc attttactaa ggccagtttt
    14701 gatgccgtca ccaatcagca tgtcatcttc tatcagtaaa attcgcattt tttcatccct
    14761 gcgataaccg tatttgctgc cagtaaaccg tgttgtaacg cgctctgtac agcattaaat
    14821 tttatttttt tcccttaaga agttgttaag gactatcttg ttaaatgctc ggaaacagac
    14881 attaaaggga gtaataaaca tgaaaaaatt cgcagcagta atcgcagtaa tggccctgtg
    14941 cagtgcaccg gtgatggcag cagagcaggg cggtttttct ggcccatcgg caacgcaaag
    15001 tcaggccgga ggatttcagg ggccgaacgg cagcgtaacg actgtagaaa gcgtaaaatc
    15061 cctgcgtgac gacacctggg taaccctgcg cggcaatatc gttgaacgca tctctgacga
    15121 tctctacgtg tttaaagatg ccagcggtac tatcaatgtt gatatcgatc acaaacgctg
    15181 gaacggtgtg acggtgacgc cgaaagatac ggttgagatt cagggtgaag tcgataaaga
    15241 ctggaattct gttgaaattg acgtcaaaca gatccgcaaa gtaaatccgt aattgttacc
    15301 gctcccggga cgcgttcccg ggaataattt cgcagggagg caaaatgaca aacctgacac
    15361 tggatgtaaa cattatcgat ttcccatcaa tccctgtggc gatgttgccg caccgctgta
    15421 gccctgagtt gctcaactac agtgtggcga aatttatcat gtggcgtaaa gagacggggc
    15481 tttctcctgt taaccaaagc cagacttttg gcgtcgcctg ggacgaccct gccaccaccg
    15541 caccggaagc gtttcgcttt gatatctgcg gcagcgttag cgaaccgatt cccgataatc
    15601 gttatggtgt gagcaatggt gaacttaccg gtggacgtta tgccgtggcc cgccacgttg
    15661 gcgagctgga cgatatttca cacacgatat ggggcattat tcgccactgg ctgcctgcaa
    15721 gcggcgagaa aatgcgtaaa gcaccgattc tgtttcacta caccaatctt gccgaagggg
    15781 tgacagagca gcgactggaa acggatgttt atgtgccgtt ggcgtgatgg ggaatcttta
    15841 ccggagtgtg agccaaatca ctcgcgataa gtttgagcga acaatgtcac aggcgtattg
    15901 ttgttgggct tataatttct ccggtaagga ataccatgta tacgcgaaat ttattatccg
    15961 gtgcgccgta agccgtctcg ggggtatcca tgagcaagcc gcacttatgg caggaggcgc
    16021 ggggcactgg tggagacaca gctaaactgg gctgaaccgt atacttacag tcgactactt
    16081 tgatgcttta ggcttccctg gactcgcata acctcaactt ctcgaatcgc ccggagcgga
    16141 cccccatgcc gagtggtgtg gcatgggcgc aaccttgtgc tgccctctat gccgatcaag
    16201 tctacagcaa agagttgtat atcaaggcac attgatgcct ttatcgccgt cggagcacgt
    16261 ccggcggcgc tattcacgct gatctcctgt gactcgacgc ggcagataat gtagtatctc
    16321 cggcaatatt gcccctttga aggctggcga ataagttgag gaatcagaat taatgagcga
    16381 tatggcagag cgccttgcgc tacatgaatt tacggaaaac gcctacttaa actactccat
    16441 gtacgtgatc atggaccgtg cgttgccgtt tattggtgat ggtctgaaac ctgttcagcg
    16501 ccgcattgtg tatgcgatgt ctgaactggg cctgaatgcc agagccaaat ttaaaaaatc
    16561 ggcccgtact gtcggtgacg tactgggtaa ataccatccg cacggcgata gcgcctgtta
    16621 tgaagcgatg gtcctgatgg cgcagccgtt ctcttaccgt tatccgctgg ttgatggtca
    16681 ggggaactgg ggcgcgccgg acgatccgaa atcgttcgcg gcaatgcgtt acaccgaatc
    16741 ccggttgtcg aaatattccg agctgctatt gagcgagctg gggcagggga cggctgactg
    16801 ggtgccaaac ttcgacggca ctttgcagga gccgaaaatg ctacctgccc gtctgccaaa
    16861 cattttgctt aacggcacca ccggtattgc cgtcggcatg gcgaccgata ttccaccgca
    16921 taacctgcgt gaagtagctc aggcggcaat cgcattaatc gaccagccga aaaccacgct
    16981 cgatcagctg ctggatatcg tgcaggggcc ggattatccg actgaagcgg aaattatcac
    17041 ttcgcgcgcc gagattcgta aaatctacga gaacggacgt ggttcagtgc gtatgcgcgc
    17101 ggtgtggaag aaagaagatg gcgcggtggt tatcagcgca ttaccgcatc aggtttcagg
    17161 tgcgcgcgta ctggagcaaa ttgctgcgca aatgcgcaac aaaaagctgc cgatggttga
    17221 cgatctgcgc gatgaatctg accacgagaa cccgacccgt ctggtgattg tgccgcgttc
    17281 caaccgcgtg gatatggatc aggtgatgaa ccacctcttc gctaccaccg atctggaaaa
    17341 gagctatcgc attaacctca atatgatcgg tctggatggt cgtccggcgg tgaaaaacct
    17401 gctggaaatc ctctccgaat ggctggtgtt ccgtcgcgat accgtgcgcc gccgactgaa
    17461 ctatcgtctg gagaaagtcc tcaagcgcct gcatatcctg gaaggtttgc tggtggcgtt
    17521 tctcaacatc gacgaagtga ttgagatcat tcgtaacgaa gatgaaccga aaccggcgct
    17581 gatgtcgcgg tttggcctta cggaaaccca ggcggaagcg atcctcgaac tgaaactgcg
    17641 tcatcttgcc aaactggaag agatgaagat tcgcggtgag cagagtgagc tggaaaaaga
    17701 gcgcgaccag ttgcagggca ttttggcttc cgagcgtaaa atgaataacc tgctgaagaa
    17761 agaactgcag gcagacgcgc aagcctacgg tgacgatcgt cgttcgccgt tgcaggaacg
    17821 cgaagaagcg aaagctatga gcgagcacga catgctgccg tctgaacctg tcaccattgt
    17881 gctgtcgcag atgggctggg tacgcagcgc taaaggccat gatatcgacg cgccgggcct
    17941 gaattataaa gcgggtgata gcttcaaagc ggcggtgaaa ggtaagagta accaaccggt
    18001 agtgtttgtt gattccaccg gtcgtagcta tgccatcgac ccgattacgc tgccgtcggc
    18061 gcgtggtcag ggcgaaccac tcaccggcaa attaacgttg ccgcctgggg cgaccgttga
    18121 ccatatgctg atggaaagcg acgatcagaa actgctgatg gcttccgatg cgggttacgg
    18181 tttcgtctgc acctttaacg atctggtggc gcgtaaccgt gcgggtaagg ctttgatcac
    18241 cttaccggaa aatgcccatg ttatgccgcc ggtggtgatt gaagatgctt ccgatatgct
    18301 gctggcaatc actcaggcag gccgtatgtt gatgttcccg gtaagcgatc tgccgcagct
    18361 gtcgaagggc aaaggcaaca agattatcaa cattccatcg gcagaagccg cacgtggcga
    18421 ggatggtctg gcgcaactgt acgttctgcc gccgcaaagc acgctgacca ttcatgttgg
    18481 gaaacgcaaa attaaactgc gtccggaaga gctacagaaa gtcactggcg aacgtggacg
    18541 ccgcggtacg ttgatgcgcg gtttgcagcg tatcgatcgt gttgagatcg actctcctcg
    18601 ccgtgccagc agcggtgata gcgaagagta atatgccggg gagggcgctg tttcctcccg
    18661 ctagcaagga acgccggatg aaacgttaag tcgtcatccg gcagcgtatt caacccccgg
    18721 aaacgataaa aattcccttg aaaccgttgt ttattcatgc gttgcgatta acaatacgct
    18781 tttccagaga gcggctttta acaatgccct aacctgattt caggtgacgt acaatgccag
    18841 tctctcagac cttcagaggg tgttatgcta tatatctttc gtcttattat taccgtgatt
    18901 tacagcatct tagtctgtgt attcggctcc atttactgcc ttttcagccc gcgtaacccg
    18961 aaacatgtgg ccacctttgg gcatatgttt ggccgtcttg cgccactgtt cggcctgaaa
    19021 gttgagtgcc gcaaacctgc agatgctgaa agctacggca atgctatcta tatcgctaac
    19081 caccagaaca actatgacat ggtgacagca tcgaacattg tgcaaccgcc gacggtgacg
    19141 gtaggtaaaa agagcttgct gtggatcccc ttcttcgggc agttgtactg gttaaccggc
    19201 aacttattga tcgacagaaa caatcgcact aaagctcacg gcaccattgc ggaagtcgtg
    19261 aatcatttca aaaaacgccg tatttccatc tggatgttcc cggaaggaac ccgcagccgt
    19321 ggtcgcggcc tgctaccgtt caagactgga gcatttcacg cggcaattgc ggcgggcgtc
    19381 ccgattattc ccgtgtgcgt ctctacaact tcgaataaga ttaatcttaa tcgactgcac
    19441 aacggtctgg tgattgtcga aatgctgccg ccaattgacg tcagtcagta tggcaaagat
    19501 caggttcgtg agctggctgc ccattgtcgt tcgataatgg aacaaaaaat cgccgagctc
    19561 gataaagaag tcgcagaacg cgaagccgcc ggaaaagttt aagtcggcaa tctgtatttt
    19621 tgcggggaac actttcctgc acggtattac tttagccagt tttacatgga gcaaatatgt
    19681 cactcagtcg gcgtcagttc attcaggcat cggggattgc actttgtgca ggcgctgttc
    19741 ccctgaaggc cagcgcagcc gggcaacagc aaccgctacc cgttccgccg ctacttgaat
    19801 ctcgccgtgg gcaaccgctg tttatgactg tacaacgtgc gcactggtca tttacgccag
    19861 ggacacgcgc gtcggtctgg ggaatcaatg gtcgttacct ggggccgact atccgcgtct
    19921 ggaagggcga cgatgttaag cttatttaca gcaaccgcct gacagaaaat gtctcaatga
    19981 cggtggccgg gctacaggta ccaggcccgc tgatgggcgg tccggcacgg atgatgtcgc
    20041 caaacgctga ctgggcaccc gtactgccta ttcgccagaa cgcagctact ctgtggtatc
    20101 acgccaatac tcccaaccgc acggctcagc aggtctataa cggccttgcc ggaatgtggc
    20161 tggtggaaga tgaagtcagc aagtcgctgc ctatccccaa ccattatggt gtggatgatt
    20221 ttccggtcat tatccaggat aaacggctgg ataactttgg tacgccagaa tacaacgaac
    20281 cgggaagcgg cggctttgtt ggtgatacgc tgctggttaa cggtgtacaa agcccgtacg
    20341 ttgaagtctc gcgtggctgg gtgcgcttgc gactgctgaa cgcgtcgaac tctcgtcgct
    20401 atcaactaca gatgagcgat ggtcgcccgt tacatgtgat ttctggcgat cagggattcc
    20461 tgcctgctcc tgtatcggtg aagcaacttt cgctggcacc gggcgagcgc cgcgagattc
    20521 tggtggatat gagcaacggc gatgaagtgt cgatcacctg tggcgaagcg gcgagcattg
    20581 ttgatcgtat tcgcggcttc tttgagccat ccagtattct ggtttctacc ttggtgctaa
    20641 cgctgcgccc aaccggcctt ctgccgctgg tcacagacag tcttccgatg cgcttgctgc
    20701 caactgaaat catggctggt tcgccaattc gcagtcgcga tatcagtctg ggtgatgacc
    20761 cgggtattaa tggacagctg tgggacgtca accgtattga tgtcaccgcg cagcaaggaa
    20821 cgtgggaacg ctggacggta cgcgcggacg agccgcaagc gttccatatt gaaggcgtaa
    20881 tgttccagat ccgtaacgtg aatggcgcga tgccgttccc ggaagacaga ggctggaaag
    20941 ataccgtttg ggttgacgga caagtggagc tgcttgttta tttcggtcag ccttcctggg
    21001 cgcacttccc gttctacttc aacagtcaga cgctggaaat ggcggaccgt ggctcgattg
    21061 ggcaactgtt ggtcaatccg gtaccgtaat cttcatactc gcccgaggag ggcgagtatg
    21121 gaataccttt cgatttctct ttcgttccgg cttataatcc ccggcctttg attatttttt
    21181 tatcatttct ggaagcaaaa tgagctctat ctccctgatc caaccggatc gcgacctgtt
    21241 ctcctggccg cagtactggg ccgcctgttt tggaccggca ccgtttttgc cgatgtctcg
    21301 tgaagagatg gatcaacttg gctgggatag ctgcgacatc attttggtta ctggcgacgc
    21361 gtatgtcgat cacccaagct tcgggatggc gatttgcggt cgtatgctgg aagcgcaggg
    21421 ctttcgcgtc gggatcatcg cccagccgga ctggagcagc aaagacgact ttatgcgtct
    21481 gggtaaaccg aatctgtttt ttggtgttac tgctggcaac atggattcga tgatcaaccg
    21541 ctataccgcc gatcgccgtt tacgtcatga tgatgcctac acgccagata atgtcgcggg
    21601 taagcgaccg gatcgcgcca cgctggttta tacccagcgt tgtaaagagg cgtggaaaga
    21661 tgtgccggtg atcctcggcg gtattgaggc tagcctgcgc cgtaccgcgc attatgatta
    21721 ctggtccgat accgtgcgcc gttccgttct ggtagattcg aaagccgaca tgctgatgtt
    21781 tggtaacggt gaacgtccgc tggtggaagt agcgcatcgt ctggcgatgg gcgagccgat
    21841 tagtgaaatc cgcgatgtgc gtaataccgc aattatcgta aaagaggcgc tgcctggctg
    21901 gagcggtgtg gattccaccc gtcttgatac cccagggaaa atcgacccaa tcccgcatcc
    21961 gtatggcgaa gatttgccgt gtgcggataa caaacctgtt gcgccgaaaa agcaggaagc
    22021 gaaagctgta atcgtgcagc caccgcgcca gaaaccgtgg gaaaaaacct acgtattgct
    22081 gccttctttc gagaaagtga agggcgataa agtgctgtac gcccatgctt cgcgcattct
    22141 gcaccacgaa accaacccag gctgcgcccg cgcattgatg caaaaacacg gtgaccgcta
    22201 tgtgtggatc aacccgcctg ctatcccgct ttctaccgaa gagatggact gcgtttttgc
    22261 gctgccgtac aagcgcgtgc cacatccggc ctatggcaat gcccgtattc cggcttacga
    22321 aatgatccgt ttttcggtca acattatgcg cggctgcttt ggcggctgtt ctttctgttc
    22381 tattaccgag cacgaagggc gcattattca gagccgttcg gaagattcga tcattaatga
    22441 gatcgaagcg atccgcgaca ccgttccagg ttttacgggc gtgatttccg atctcggtgg
    22501 gccaactgcc aacatgtata tgttgcgctg caaatcgcca cgcgctgaac aaacttgtcg
    22561 ccgtttgtcg tgcgtttatc cggatatttg tccgcacatg gacactaacc acgaaccgac
    22621 gatcaacctc tatcgccgtg cgcgtgatct gaaaggcatt aaaaaaatcc ttatcgcctc
    22681 aggcgtacgt tatgacatcg ccgtggaaga tccgcgctat atcaaagagt tggcgaccca
    22741 tcacgtcggc ggttatctga agattgctcc ggaacatacc gaagaagggc cgttgtcgaa
    22801 gatgatgaag ccgggcatgg gcagctatga ccgctttaaa gagctgttcg atacctactc
    22861 gaaacaggca ggtaaagagc agtatctgat cccgtatttc atctctgcgc accccggtac
    22921 gcgtgatgaa gatatggtga atttggcgct gtggctgaaa aagcatcgtt tccgtctcga
    22981 ccaggtacag aacttctacc catcgccgct ggcgaactca accaccatgt attacaccgg
    23041 gaaaaaaccg ctggcgaaga ttggttataa gagcgaagac gtcttcgtac cgaagggcga
    23101 caaacagcgt cgtttgcata aagcgttgtt gcgttaccac gatccggcaa actggccgtt
    23161 aatccgccag gcgctggaag cgatgggcaa aaagcatctg attggcagcc cgcgattgct
    23221 tagtgcctgc gccaaccatt gaagagatgc gtgaagctcg tcgccagaac cgcaataccc
    23281 gtccggcgtt gactaaacat acgccgatgg cgactcagcg tcagacgcct gctacggcaa
    23341 aaaaagcgtc gtctacgcaa tctcgtccgg tgaatgctgg tgcgaagaaa cgccctaaag
    23401 cggcggttgg acgttaaaaa aaatccccgg caactgacat gctgccgggg atttttttat
    23461 cattctgcgt acatacgcaa gcagcgcagg caatcaaaat agtcctgtgc atcaagacgc
    23521 gtattggctc tgttagcttc ggatttggca gcatagattt gtgggcgtag tgctttcata
    23581 tcctgctcaa gccgggcgat tttggcattt aatgtttgta cattgctcgc cagttgattg
    23641 accttttgct cattgacgca gccactgagc aagcaggttg ccagaacaac agctcccacg
    23701 gtgaaaatcg ttttcatata gtgttccctt ttatttattg ttccggctaa ttgttattta
    23761 attcagctca ttgctttaat ctggcggcgg tttttgcctc aatcagctta agtaggctgc
    23821 cgctgttcgg gcggataaaa tcagcgattt cccacttacc ttcgcgccgc tgtaaaacca
    23881 gctcttgctc ttcattgttg ccattggtca gcgtaaaacg aacatgaatg cgggcggaat
    23941 cggcggtttt ctccaggact cgaatttcat caacgcgtga aaatagcgac tcttcgccaa
    24001 aacagatcgg attgtcgtaa ataaattgcc gttctatttg catatcttca tcgggattat
    24061 tggccgtctt gaaggtggtt ttatagaagg cattaaccgc ctggcggtaa gagtcggaaa
    24121 ccacccacgt ccacggccag ctataatcag caatatgttc aaagagttca gcaacaaagg
    24181 attctggctg ttctttctgc aacgaagcta aagcggccag cgttttttca ttctcgctat
    24241 taactgcttg taagacgctg ccatgattgc tgacaatatc atcaatgacc caacgaccat
    24301 tttccgccac cattttcagt gtctgtgtgg tcttttcttt atcatcttta aagatacgaa
    24361 agcgcacaac ggcatcggca tgatcgacgt cagtttgggt tatcgctacg ctttctaaca
    24421 ccagatcgcc aaaatcctga caatcacaaa ccggatcata atccagccag ccaatattgc
    24481 ccggtagtgt aagattgttg ttcagggtaa gcgcctgttg aatacgcgct gaagttatcg
    24541 cccgctctcc ggtttcaccg aaataaggcg tactggcatc tgatttatag ttctgataaa
    24601 tctgccggac tgtttgttct acggtctgtg aaggaggctg cgcgtgtacg gtaaaacttg
    24661 ccagggctgc taaaaacaga agtattattt tcatctgttt ttcacctctc cgttgcgttt
    24721 atcccggata tagatgagtt gttcttttaa gagcttccgg ctctgcatga tgatgtcctt
    24781 atatttggca ttcctgaaca aggaagagta acaacgggcg ggacgcgagg ggaataaatg
    24841 atttctgaaa agtccggtag cggaacatta ccgccaccgg gagaatttgc acgttagccg
    24901 ccgaactggt caggatcggg accgagacgc ttgccctgat cgagttttgc aattttgccg
    24961 agttcgtctt tgtcgagacg gaaatcccag acatcaaagt tttcggcaat acgtgaaggt
    25021 gtgaccgatt tcgggatcac caccaggccg ctatccagat gccagcggat aacaatctgc
    25081 gccggggttt tgccgtattt atctgccaga tcgcgaatga ctttctgatc gaaaacgcct
    25141 tttcctcctt gcgctaatgg gctccaggat tcggtctgga ttttgtgtgt cgcgttccag
    25201 gcgtgtagct ggcgttgttg catcagcgga tgaagttcga tctggtttat cacaggcgtc
    25261 acgccagttt catcaatcag gcgttgcagg tgatggatct ggaagttgca cacgccgatg
    25321 cttttgatta atccctcttt ttgcaattcg atcatgcctt tccatgcttc gacataatgg
    25381 tcgatagcgg gaacgggcca gtgcattaag tagaggtcga tataatcaag ctggagtttt
    25441 ttcaggctgt cgagcagggc ttcgcggggg cacttgtggt cgtcgttcca cagcttagtg
    25501 gtgatgaaca gttcttctct gttgactgag gcatttttca gggctttgcc gacaccttct
    25561 tcgttcttgt aggccgcggc ggtatcaatc gagcgataac ccacttctaa cgctttttga
    25621 atggcggtga ttacttcctc attacttgct tgccagacgc ccagtcccag ctggggcatg
    25681 acattgccat cctgtagctt aataacggtt ggattagcca tacgttcctc ctttatatga
    25741 actcaccgga gcaggctccg gtgaggtgtt gtgaacttaa gtctggacga aatgcccgaa
    25801 aacgaaagtt tgaggcgtaa aaagcttagc gggcggcttt gtatatacgg cggctgacat
    25861 ccaacgtaat gtcatgattt tcgcccagtt gggtcatgcc gtgctcttcc agttttttca
    25921 gcaaagccgg gatggagctg ccgtccagac cgtagtcgga gaggtgggtc ggcacgccta
    25981 attgctcaaa gaaattgcgg gttgcggcaa tcgcggcgtc aatacgctca tcgtcggaac
    26041 cttcagtgat gttccagacg cgttcagcat attgcagcag cttagcgcgc ttggtctcgc
    26101 gtttttcatt ccacagtgca ggcaggacga tagccagtgt ttgcgcgtga tccagaccgt
    26161 gcatcgcagt cagttcgtgg cccagcatat gcgttgccca gtcctgcggt acgccagcgc
    26221 caatcaaacc gttcagcgcc tgagtcgccg cccacatgac gttggcgcgc acatcgtagt
    26281 tttctggctc tttcagggct ttcggaccat cttcgattag cgtcagcaaa atgccttctg
    26341 cgaaacggtc ctgaattttg gcatcaaccg gtttggtaac atactgttcc acggtgtgta
    26401 caaaggcgtc cactacgccg ttagccacct gacgcggcgg cagggtgtag gtataaaccg
    26461 gatcgagcac ggcaaatacc ggctgaacat gtgcagaatg gaacgcctgc ttgtcgcctg
    26521 tggttttacg ggagatcacc gcgcctgcgt tggattctga accggttgct ggcagcgtca
    26581 gcacacagcc catcgggatg gcgcttttaa tctctttacc gcccgtttgc agaatgtgcc
    26641 acggatcgat attttccgga tagttagccg ctgcggcgat aaatttggtg ccgtccagta
    26701 cagaaccgcc gccaaccgcc agcaggaaag tcactttctg ttcgcgaacc agtttcacgg
    26761 cgttcatcag cgtttcataa gccgggtttg gctcaatacc gccaaattcc agcacgtcca
    26821 tgcctttcag ggcatccaga acttgatcga gaacgccggt ttttttcacg ctgccgccac
    26881 cgtaggtaat caatacgcga gcatcgtgag gaatttgttc gcgtaaacca gcgattgcgc
    26941 ctttaccaaa cagaatgcgg gttggggtgt gcagattaaa gttgttcatt acttgctccc
    27001 tttgctgggc caatatgagg gcagagaacg atctgcctga tgtttttcat tgtgatcgcc
    27061 agcgccctag ctctcaatgc tcatttctgc caatgtcttg cctatttctc cagagtgctg
    27121 gagaaatgct acaaaat
//
LOCUS       DAJTYI010000043        26931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYI010000043 DAJTYI010000000
VERSION     DAJTYI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..764
                     /gene="pdeC"
                     /locus_tag="OO564_001794"
     CDS             <1..764
                     /gene="pdeC"
                     /locus_tag="OO564_001794"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6652944.1"
                     /translation="QRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPA
                     EFIPLAEKEGMIEQITDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARIN
                     QKTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLK
                     SLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKR
                     GVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(767..1090)
                     /gene="soxS"
                     /locus_tag="OO564_001795"
     CDS             complement(767..1090)
                     /gene="soxS"
                     /locus_tag="OO564_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6652945.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1176..1640
                     /gene="soxR"
                     /locus_tag="OO564_001796"
     CDS             1176..1640
                     /gene="soxR"
                     /locus_tag="OO564_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6652946.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2344..3693
                     /gene="ghxP"
                     /locus_tag="OO564_001797"
                     /gene_synonym="yjcD"
     CDS             2344..3693
                     /gene="ghxP"
                     /locus_tag="OO564_001797"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6652947.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3844..5493
                     /locus_tag="OO564_001798"
     CDS             3844..5493
                     /locus_tag="OO564_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6652948.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5647..6939)
                     /locus_tag="OO564_001799"
     CDS             complement(5647..6939)
                     /locus_tag="OO564_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6652949.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7117..8766)
                     /gene="actP"
                     /locus_tag="OO564_001800"
     CDS             complement(7117..8766)
                     /gene="actP"
                     /locus_tag="OO564_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6652950.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(8763..9077)
                     /locus_tag="OO564_001801"
     CDS             complement(8763..9077)
                     /locus_tag="OO564_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6652951.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9277..11235)
                     /gene="acs"
                     /locus_tag="OO564_001802"
     CDS             complement(9277..11235)
                     /gene="acs"
                     /locus_tag="OO564_001802"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6652952.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            11628..13064
                     /gene="nrfA"
                     /locus_tag="OO564_001803"
     CDS             11628..13064
                     /gene="nrfA"
                     /locus_tag="OO564_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6652953.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGVTEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13109..13675
                     /gene="nrfB"
                     /locus_tag="OO564_001804"
     CDS             13109..13675
                     /gene="nrfB"
                     /locus_tag="OO564_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6652954.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            13672..14343
                     /gene="nrfC"
                     /locus_tag="OO564_001805"
     CDS             13672..14343
                     /gene="nrfC"
                     /locus_tag="OO564_001805"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6652955.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14340..15296
                     /gene="nrfD"
                     /locus_tag="OO564_001806"
     CDS             14340..15296
                     /gene="nrfD"
                     /locus_tag="OO564_001806"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6652956.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15316..17034
                     /locus_tag="OO564_001807"
     CDS             15316..17034
                     /locus_tag="OO564_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6652957.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17027..17410
                     /gene="nrfF"
                     /locus_tag="OO564_001808"
     CDS             17027..17410
                     /gene="nrfF"
                     /locus_tag="OO564_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6652958.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17407..18003
                     /gene="nrfG"
                     /locus_tag="OO564_001809"
     CDS             17407..18003
                     /gene="nrfG"
                     /locus_tag="OO564_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6652959.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18345..19658
                     /gene="gltP"
                     /locus_tag="OO564_001810"
     CDS             18345..19658
                     /gene="gltP"
                     /locus_tag="OO564_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6652960.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(19736..20425)
                     /locus_tag="OO564_001811"
     CDS             complement(19736..20425)
                     /locus_tag="OO564_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6652961.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20519..22666)
                     /gene="fdhF"
                     /locus_tag="OO564_001812"
                     /pseudo
     CDS             complement(20519..22666)
                     /gene="fdhF"
                     /locus_tag="OO564_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(22864..24330)
                     /gene="mdtP"
                     /locus_tag="OO564_001813"
     CDS             complement(22864..24330)
                     /gene="mdtP"
                     /locus_tag="OO564_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6652962.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24327..26378)
                     /gene="mdtO"
                     /locus_tag="OO564_001814"
                     /pseudo
     CDS             complement(24327..26378)
                     /gene="mdtO"
                     /locus_tag="OO564_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26378..>26931)
                     /locus_tag="OO564_001815"
     CDS             complement(26378..>26931)
                     /locus_tag="OO564_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HCR6652963.1"
                     /translation="AKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADIALTKL
                     HLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSG
                     TPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
                     MVDKPDPEMFRIGASAVANLEPQ"
BASE COUNT         6091 a         6845 c         7288 g         6707 t
ORIGIN      
        1 tgcaacgcgc cctcgaaaaa catcaacttt gtctttatta ccagccaata atcgatatca
       61 aaacagaaaa atgtatcggc gctgaagcgt tgttacgttg gcctggtgag caggggcaaa
      121 taatgaatcc ggcagagttt attccgctgg cagaaaagga ggggatgata gaacagataa
      181 ctgattatgt tattgataat gtcttccgcg atctgggcgc gtacctggca acacatgccg
      241 atcgctatgt ttctattaac ctgtcggcct ccgattttca tacgtcacgg ttgatagcgc
      301 gaatcaatca gaaaacagag caatacgcgg tgcgtccgca gcaaattaaa tttgaagtga
      361 ctgagcatgc atttcttgat gtcgaaaaaa tgacgccgat tattctggct ttccgccagg
      421 ctggttacga agtggcaatt gatgattttg gtattggcta ctctaacttg cataacctta
      481 aatcattgaa tgtcgatatt ttgaaaatcg ataaatcgtt tgttgaaacg ctgaccaccc
      541 acaaaaccag tcatttgatt gcggaacaca tcatcgagct ggcgcacagc ctggggttaa
      601 aaacgatcgc tgaaggcgtc gaaactgagg agcaggttaa ctggctgcgc aaacgcggcg
      661 tgcgctattg ccagggatgg ttttttgcga aggcgatgcc gccgcaggtg tttatgcaat
      721 ggatggagca attacccgcg cgggagttaa cgcgcgggca ataaaactac aggcggtggc
      781 gataatcgct gggagtgcga tcaaactgcc gacggaaaac gcgggagaag gtctgctgcg
      841 agacataacc caggtccatt gcgatatcaa aaatcggacg ctcggtggtg cgcaactcaa
      901 cggcggccag taacaggcgg cgttggcgaa tgtaatcgcc aagcgtctga tgcgtcaccg
      961 tgcggaacat ccgttgcaag taccactttg aatagcctga tttttttgcg acgacatcaa
     1021 tgttaagcgg ctggtcaata tgcacgtcaa tccatgcgat aagatcctga ataattttct
     1081 gatgggacat aaatctgcct cttttcagtg ttcagttcgt taattcatct gttggggagt
     1141 ataattcctc aagttaactt gaggtaaagc gatttatgga aaagaaatta ccccgcatta
     1201 aagcgctgct aacccctggc gaagtggcga aacgcagcgg tgtggcggta tcggcgctgc
     1261 atttctatga aagcaaaggg ttgattacta gtatccgtaa cagcggcaat cagcggcgat
     1321 ataaacgtga tgtgttgcga tatgtcgcaa ttatcaaaat cgctcagcgt attggcattc
     1381 cgctggcgac cattggtgaa gcgtttggcg tgttgccaga agggcatacg ttaagtgcga
     1441 aagagtggaa acagctttcg tcccaatggc gagaagagtt ggatcggcgc attcatacct
     1501 tagtggcgct gcgtgacgaa ctggacggat gtattggttg tggctgcctt tcgcgcagtg
     1561 attgcccgtt gcgtaacccg ggcgaccgct taggagaaga aggtaccggc gcacgcttgc
     1621 tggaagatga acaaaactaa agcgccacaa ggacaagctg actcatcgtt aactatgagg
     1681 ccggggtgag ggtaaatatt tgcactggtg ctgcctgttc ccctcaccct aaccctctcc
     1741 ccgaaggggc gaggggactg atcgagctct tttggaatta tccgcaaaca caaaactaaa
     1801 gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctgga
     1861 acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg
     1921 gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac
     1981 tttttagccg caatattttt gtcatctcag acgatttttt atcgcaatcc agaacggtat
     2041 acggctcgat aacgctgcaa tcttgcacat cgacgataac gtttgcgcat caattgcctg
     2101 gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg
     2161 ctatgatcgc cggataatcg tttgcttttt taccacccgt tttgtatgcg cggagctaaa
     2221 cgtttgcttt tttgcgacgc accaaattgc cgcaaacctg gagcaggaag ataacgtttc
     2281 gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag gggatgtttt
     2341 cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt taaaatttca
     2401 caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt tctggcgatg
     2461 gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc gcctgcggca
     2521 gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg tctgtgggct
     2581 aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc attcagcctg
     2641 gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct gatgggggtg
     2701 ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa cttgccgcac
     2761 ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat tgccgctaat
     2821 ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct gggtgatttc
     2881 gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg cttggaaaaa
     2941 ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt cggtttgatc
     3001 ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag cgatgaaaac
     3061 ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt agtcctgcca
     3121 agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat ccgcgctgtc
     3181 gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggcgg gaaagcactg
     3241 accactgact ccatgagcag cgttttctct ggcctggtgg gtgcagctcc ggcagcggta
     3301 tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccgggttgac ggctatcacc
     3361 gttggcgtgc tgttcctgct gattctgttc ctctctccgc tttcttacct cgttccgggg
     3421 tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa cgtggcgaaa
     3481 atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt attcatcgtg
     3541 ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt gattggtcgt
     3601 ctggtttccg gtgaatggcg caagttgaac atcggtacgg tcgttatcgc cgtggcgctg
     3661 gtgaccttct atgcgggggg ctgggctatc taatctttca tcggattctg aaaacgggtg
     3721 gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg atattctgaa
     3781 aaaatgagaa ttcaggcata acgtctttct cagatcataa aaaacatcgc aaacagggaa
     3841 cgcatggaaa tcttcttcac catactgata atgaccctcg tggtctcgct gtccggggtg
     3901 gtcactcgtg tcatgccctt tcagatcccg cttccgctta tgcaaatcgc catcggtgcg
     3961 ctactggcgt ggccgacgtt tggtttgcat gtggagtttg atcctgaact ctttttagtc
     4021 ttgtttatcc cgccgttgct gttcgctgat ggctggaaaa cgccgacccg tgaatttctc
     4081 gaacatggtc gagagatttt cggcctcgcg ctggcgctgg tggtggtcac cgtggtcggc
     4141 attggcttcc ttatttactg ggtggtgccg ggcattccgc tgatccccgc ctttgcgctg
     4201 gcggcggtac tttctccgac cgatgctgtg gcgctctccg ggattgttgg cgaagggcgc
     4261 atcccgaaaa aaatcatggg cattttgcag ggcgaagcgt tgatgaatga cgcttccggc
     4321 ctggtgtcgt tgaagtttgc cgtggcagtg gcgatgggga cgatgatctt cactgtcggc
     4381 ggtgcgacgg tcgaatttat gaaagtagcc attggcggta ttcttgccgg ttttgtggtg
     4441 agctggctgt atggtcgctc gctgcgattc ctcagccgct ggggcggtga tgaacccgcg
     4501 acgcagatcg ttctgctgtt cttgctgcca ttcgcttctt atctgattgc cgaacatatt
     4561 ggcgtttcgg gcatcctcgc tgcggttgcc gccgggatga ccatcacccg ctccggtgtg
     4621 atgcgccgtg cgccactggc aatgcgcctg cgcgcaaaca gcacctgggc gatgctggaa
     4681 tttgtcttta acggcatggt gttcctgctg ttaggtctgc aactgccggg tattctggag
     4741 acgtcgctga tggcggcaga aatcgaccct aacgtcgaaa tctggatgct gtttaccgat
     4801 attattctga tatatgcggc gctgatgctg gtccgtttcg gctggctgtg gacgatgaaa
     4861 aagttcagca accgcttcct gaagaagaag ccgatggagt ttggttcgtg gaccacacga
     4921 gaaatcctga tcgcgtcttt cgctggggtg cgtggggcga tcactctggc cggtgtgctc
     4981 tcaatcccgc tgctcttgcc ggatggtaac gtcttcccgg cgcgctatga gctggtgttc
     5041 ctggcggctg gcgtcattct cttctcgctg tttgtcggcg tggtgatgtt gcctattctg
     5101 ctacaacaca ttgaagtcgc ggaccattcg caacaattga aagaagaacg tattgcgcga
     5161 gcggcaacgg cagaagtggc gattgtggcg atccagaaaa tggaggagcg tctggcggcg
     5221 gataccgaag agaatatcga taaccagttg cttacggagg tcagttctcg cgtcattggt
     5281 aacctgcgtc gtcgcgccga tggacgtaac gacgttgaaa gttccgtgca ggaagagaac
     5341 cttgagcgtc gcttccgtct ggcggcattg cgttctgaac gtgctgaact gtaccacctg
     5401 cgcgccacgc gggagatcag caacgaaacg ctgcaaaaat tactgcacga tctcgatttg
     5461 cttgaagcgt tgctaattga ggaaaatcag taagccgaaa gcgacgtaac caggcccgag
     5521 gtaatctgaa aaaggtgcaa tcttttgcac ctttaacagg ttagctcggg cgaacattca
     5581 tgattctaaa acaaataagt tatctctctt gtaaagtcaa ttcattggct gttcttcgac
     5641 ttaacctcaa tctgcgtgtt gcataatatc tgcagtaatc ttcatagcga ctgcattaat
     5701 attatcctca gtttgtgaca tgccagtatg agaggtacaa tcttcttcaa agaatttctg
     5761 tagtgttacc agggtatcac tatcaatggg gatattttcc tccccacgga gaatggaaat
     5821 gcccattccc tggacgctag cctcatcgaa attaacccga tagagacggg tattaaacat
     5881 acaggctttt gttaaaatag aacagttgaa atggcagttg cttaaattgg cattattaaa
     5941 attaacatta gccatattag agccggtaca tataaccatt tcgaggaatg tatttgtcag
     6001 ccacgtacaa aagagtttgc tatcaattaa gtttatattc tggaggattg ctttgttcag
     6061 attgacgcga gacagatcag catgttcaaa gttaattaaa tcgagtttag agaaactaag
     6121 gtcggcttta aataaattaa catatggcgc tattacttta taaagattta cctcaaccat
     6181 gtacgccata taaaaatttg aatagtcaag gaaggtattt ttaatattgg ctctattcat
     6241 tgctgcattg tagaattgaa catgcatagc ggagcatgca tttagagttc cgtcagccat
     6301 ttttatctga gcgaaatttg agtcatcgag aatacaatta tcaaaaagta ttttttccat
     6361 tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag cgttttgtag
     6421 tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga tacccttcag
     6481 gctaaggtgt gaaaaatcgt aaccaccatt tactatctgt ggaatttcga tagtattatt
     6541 atggacggcg acattattta taaaatatac caccttaatg gatacatcat tttctttact
     6601 aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata acgctatatc
     6661 tataaaatat ttatgtattt tcctgtccgt ttctttcgtg atttttcctg atggtaaact
     6721 atcataaatt cttttcattt tctgaatcaa gcctatcact ttttcatgat catctatttt
     6781 gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc tgtaattatc
     6841 tgattttgtt ttctttgtat gtgatgaact tgtgatagag tggttatcgt taatctggca
     6901 aagagggaaa aacatattat ttaaaccatt atagcgcata aaatatcctt ataattaaca
     6961 agaaagaaaa ggcatattct ctgcattatc attttctacg actgtcaaaa atcgctcatt
     7021 ttttaatgag tttatttgtt taatattatg ggaaaaggtg atgcatgttg gagaggaaga
     7081 gtattccccg gtcaggcgac cggggaaggg gagagattaa tgtgcgcggc cttgctcaac
     7141 gccaaagccg gtctgggagc ggataaactg cgcgcggaac agttcacgct cgcgcgcccc
     7201 ttccgctgag ttatcggttg ccgagaagaa ccagatgccg aggaatgcca cgctgataga
     7261 gaacagcgcc gggtattcat acgggaagat ggctttttcg tgaccgagga tctgtaccca
     7321 aatagtcggg ccgaggatca tcagcaccac tgcggtaatc agccccagcc agccgcccag
     7381 catcgcgcca cgggtagtca gtttcgacca gtacatggaa agcagaatga tcgggaagtt
     7441 acagctcgcc gcgatggcaa acgcaaggcc taccataaag gcgatgttct gattctcaaa
     7501 cagcacgccg aggataatcg caatcacgcc gaggatcagt acggtgattt ttgatacccg
     7561 cagctcttca cgttcggtcg cgcctttttt gaagacgtta gcgtacaagt catgcgaaac
     7621 cgccgatgcg cccgccagcg tcagacccgc aaccaccgcg aggatagtgg cgaaagcaac
     7681 cgctgaaata aaaccgagga ataggttgcc gcccactgca ttcgccaggt gaacggccgc
     7741 catgttgtta ccaccaatca gatggcccgc cgcgtcttta tattccggat tcgcaccaac
     7801 aagcatgatc gcgccgaagc cgataataaa ggtcagaata tagaagtagc ccataaatcc
     7861 ggtggcgtag aacacgctct tacgtgcttc gcgggcatcg ctgactgtaa agaagcgcat
     7921 cagaatgtgc ggcaagcccg ccgtaccaaa catcagtccc agccccagag agagcgcgga
     7981 gatcggatct ttcaccagtc cgcccggttt catgatgtcg acacctttcg ggtgtaccgc
     8041 catcgcttcg ctgaacagat tgttgaagct aaagccgacg tgtttcatca ccataaaggc
     8101 cataaagctg gcaccgaaca gcaacagcac agctttgata atttgtaccc aggtggtcgc
     8161 cagcatgccg ccgaacagga cgtacatcat catcagcacg ccgaccagca ccaccgcaat
     8221 gtgatagtta aggccaaaca gcagctcgat cagtttacct gcgcctacca tctgagcgat
     8281 aaggtaaagc gccaccacca ccagagaacc acaggccgaa agaatacgaa tcggcccttg
     8341 tttcagacga taagaggcca catcggcaga ggtgtagcgt cccaggttac gcagacgttc
     8401 ggtaatcaga aacagaatga ttggccagcc caccaggaag cccaacgagt agatcagccc
     8461 gtcatagccg gaggtaaaca ccagcgcgga gatccccaag aatgaggcgg cggacatata
     8521 gtccccggca atcgccagcc cgttctggaa gccagtgata ttgccgcctg cggtgtagta
     8581 gtcgctacga gaacgtacgc gttttgatgc ccagtaggta atgccgagcg taaacacgac
     8641 gaaaatcagg aacataataa tcgcctgcca gttcgttggc tggcgctcta cggccccgct
     8701 aatagcatcc gcggcgttag ctgcgaaagg gagtgtggcg gcaagcgccg tcagaactct
     8761 tttcatgatg cttgtacctc atgcaggact tcattattaa gacggtcgaa ttcgccgttc
     8821 gcccgccaga tgtagatacc ggtgagaaca aaggagatca caatcactcc aacaccaatt
     8881 ggaataccgc gtgtgacgct ggtgttcgga ttcagcggcg tgcccagcca gccgggcgcg
     8941 aaggcgatca gtaaaataaa gccgatataa actgccagca taataatcga caggatggtg
     9001 gcaaaccgtt gccgtttttc gactaactcc ctgaaatgcg cattgtcttc tatccgctga
     9061 taaatcgtac cattcatcac agattctcca gaggtaatgt agggattgtt ttaattcccg
     9121 ctcccttatg ggagaaggtt aacgctcggg taacccttgc cgaatgtagg ccggataagg
     9181 cgtttacgcc gcatccggca atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct
     9241 acaaaccgtt accgattcgc atccggcaat tgtgggttac gatggcatcg cgatagcctg
     9301 cttctcttca agcagcttct cgaccacgcc aggatcggca agcgtcgagg tatcgcccag
     9361 gttgctggta tcgcccgccg caattttgcg cagaatacgg cgcataattt tgccggagcg
     9421 ggttttaggc agggagtcgg tccagtgcag cacgtctggc gtcgccagcg ggccaatctc
     9481 tttacgcacc cagttgcgga cttctgcgta cagttctggt gacggttcct ccccgtgatt
     9541 aagcgtgaca taggcgtaga tcgcctgacc tttaatattg tgcggaatac ctactacggc
     9601 ggcttcggca atcttcggat gcgccaccag cgccgactct atctctgccg tccccagacg
     9661 gtgaccggag acgttcagca cgtcgtccac acgcccggtg atccagtaat agccatcttc
     9721 atcgcgacgc gcgccgtcgc cgctgaaata catatttttg aaggtggaaa aataggtctg
     9781 ctcaaaacgt tcgtgatcgc caaacagcgt acgcgcctga ccaggccagg agtcggtgat
     9841 caccaggcta ccttcggtag ccccttccag cgggttacct tcgttatcga ccagcgccgg
     9901 ttgcacgccg aagaacggac gtgttgccga accggctttc agctcggtag cgccaggcag
     9961 cggggtgatc atgaaaccgc cggtttcggt ctgccaccag gtatcgacca ccggacattt
    10021 ctcgttgccg atttttttcc agtaccactc ccacgcttcc gggttaattg gctcgcccac
    10081 ggaaccgaga atgcgtagcg acgaacggtc ggtgccttca atcgctttat cgccttccgc
    10141 catcagcgcg cggatcgccg tgggtgcggt atagagaata ttgacctgat gcttgtccac
    10201 cacctgcgcc atacgggcag gcgtcggcca gttcggtacg ccttcaaaca tcagcgtggt
    10261 cgcgccgcag gccagcgggc cgtacagcaa ataactgtgt ccggtaaccc agcccacatc
    10321 ggcggtgcac cagtagatat cacccggatg ataatcaaag acatatttaa aggtcagcgc
    10381 cgcgtacacc agataaccgc cggtagtgtg cagtacgcct tttggttttc cggtagaacc
    10441 ggaggtatag agaataaaca gcggatcttc ggcgttcatc tcttccgcct ggtgctgatc
    10501 gctggcttgc tcaaccaggt cgtgccacca caggtcgcgc ccttcctgcc agtcgatgtt
    10561 tccgccagta cgcttcagta ccaccacatg ctctacgctg gtgacgttcg ggttttttag
    10621 tgcgtcatca acgtttttct tcagcggaat actacgcccg gcgcgcacgc cttcgtcgga
    10681 agtgatcacc agtcgtgagt tggaatcaat aatgcgcccg gcaacggctt tcggcgagaa
    10741 gccgccgaaa atcaccgaat gcaccgcgcc aatgcgggcg caggccagca tcgcaaccgc
    10801 ggcttccggc accatcggca tataaatcgc caccacatca ccttttttaa tgcccagctc
    10861 gagcagggta ttggcgaagc ggcagacgtc gcggtgcagc tctttatagc tgatatgttt
    10921 gctctggctg gcgtcgtcgc cttcccagat gatggcggtg cgatcgccgt tttcttgcag
    10981 atggcggtca aggcagtttg ccgccagatt cagcgtgccg tcctcgtacc atttaatgga
    11041 cacattaccg ggggcaaagg aggtattttt caccttctgg tacggtttga tccagtcgag
    11101 aatttttccc tgttcgcccc agaaggtatc aggtacgtta atagattgtt gatacatcgc
    11161 ctcgtactgc tgagggttta tcaggcaacg gtctgcgatg ttggcaggaa tggtgtgttt
    11221 gtgaatttgg ctcatgcttt tgttctcctt gtaggatgtt aataatatgt ggcataagcg
    11281 ttaaatgtag gggtattggc agttttgttt agtatttggg cgacagatca cgcaaaagta
    11341 gaatggtgca aataaacggc agggtaattt ttgaaggtca ccaacaaaag ttgattaatt
    11401 ctttgaggaa catgcagtta tgcatgctgt tgaaaaagag gaagatacta actaactcta
    11461 aagtggtatt ttacatgcac ttacaattga ttaaagacaa cattcacagt gtggttattt
    11521 gttacacata ggggtgagca atgtcatgac agtgtaggtg cggttactgt cgtgaaaaac
    11581 aataaaaacc gccattgcaa caatggcgca attcggatga agcccctatg acaaggataa
    11641 aaataaacgc acgccgtatc ttcagcttat tgattccttt tttctttttc acttctgttc
    11701 acgctgaaca aacggcagct cccgcaaaac ctgtaactgt ggaagcgaag aatgaaacct
    11761 ttgccccgca gcatcacgat caatatctct cctggaaagc cacctcggaa cagtcagagc
    11821 gtgttgacgc cctggaggaa gatccacggc tggtgatcct gtgggcgggg tatcccttct
    11881 cgcgcgatta caacaagccg cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc
    11941 tgcgtaccgg tgcgccgaaa aacgctgaag atggtccgct accgatggcg tgctggagtt
    12001 gtaaaagccc ggatgtggcg cgtctgatcc agaaagacgg cgaagatggc tacttccacg
    12061 gtaaatgggc gcgcggcggc ccggaaatcg tcaacaactt aggttgtgcc gactgccata
    12121 acaccgcctc accagagttc gccaaaggca aaccggagtt aaccctttcc cgtccgtatg
    12181 cggctcgcgc gatggaagcc attggtaaac cttttgagaa agccggacgt ttcgacaagc
    12241 aatcgatggt ttgcggtcag tgccatgtgg agtattactt cgacggcaaa aacaaagcgg
    12301 ttaaattccc gtgggatgac ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa
    12361 ttgccttctc tgactggact aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc
    12421 cggaatatga aacctggaca gcgggcattc acggtaaaaa caacgtgacc tgtatcgact
    12481 gccatatgcc aaaagtgcag aacgccgaag gcaaactcta caccgaccat aaaattggta
    12541 atccgtttga taacttcgcc cagacttgtg cgaactgcca tacccaggat aaagctgcct
    12601 tgcaaaaagt ggtcgcggaa cgtaagcaat cgattaacga cctgaaaatc aaggttgaag
    12661 atcaactggt tcacgctcac ttcgaagcga aagcggcgct ggatgcaggc gtgacggaag
    12721 ccgaaatgaa gccgattcag gacgatatcc gtcatgccca gtggcgttgg gatctggcga
    12781 tcgcttccca cggtattcat atgcacgcac cggaagaagg tctacggatg ctcggtacgg
    12841 cgatggataa agcggcggat gcgcgcacca aactggcgcg cctgctggcg accaaaggca
    12901 tcacccatga aatccagatc ccggatatct cgaccaaaga gaaagcccag caggccattg
    12961 gcctgaacat ggaacaaatc aaggccgaga agcaggactt catcaaaacg gtgatcccgc
    13021 agtgggaaga gcaggcacgt aaaaacggtc tgttaagcca ataaccccgt tccgcctcgc
    13081 aaggggcgga aaacacaatg gagtgaatat gagcgtatta cgttcgttat taactgctgg
    13141 ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt accgctaccc ctgcggtgca
    13201 ggcatctggt gatcgctacg aagttaccca gcagcgtaac ccggatgccg cctgtctgga
    13261 ctgtcataaa ccagataccg aaggtatgca tggcaaacat gcttccgtta tcaacccgaa
    13321 taacaaattg ccggtcacct gcaccaactg tcacggccag ccatcaccgc aacaccgcga
    13381 aggggtgaaa gatgtgatgc gctttaacga gccgatgtac aaggtcggag agcagaacag
    13441 cgtctgtatg tcctgtcatc tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt
    13501 ccacgtaacc aaagtggcgt gcgccagctg ccattccctg catccacagc aagatacgat
    13561 gcagacgtta agtgacaaag gacggattaa gatttgcgtc gattgccaca gcgatcagcg
    13621 caccaatccg aactttaacc cggcgtctgt tccgttgctt aaggagcagc catgacctgg
    13681 tctcgtcgcc agtttctcac cggcgtcggc gtgctggcag ccgtcagcgg caccgcaggg
    13741 cgcgtggtgg cgaaaacgtt gaatatcaat ggggtgcgtt acggcatggt gcatgacgag
    13801 tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg
    13861 gaaggcgtct cgcgcctgac gattattcgt agcgagccgc agggcgaatt tcctgatgtg
    13921 aaatatcgct tcttccgtaa gtcttgccag cactgcgagc atgcgccgtg cgttgacgtc
    13981 tgcccgaccg gtgcgtcttt tcgtgatgcc gctagcggga ttgtcgatgt taatcctgat
    14041 ctctgcgtcg gttgtcagta ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat
    14101 ccggtcacga agacggcgga caaatgcgat ttctgtcgta agaccaattt gcaggccggt
    14161 aagcttcccg cgtgcgttga agcttgcccg acgaaggcgc tgacgtttgg caatctggac
    14221 gatcctaaca gtgagatttc gcaactgctg cgccagaagc ctacttaccg ctacaagctg
    14281 gcgctgggaa ccaaaccgaa gctgtaccgc gtaccgttta aatacgggga ggtgagccaa
    14341 tgacgcagac ttccgcattt cattttgaat cgctggtgtg ggactggccg attgccatct
    14401 acctgttttt gattggtatt tctgccggtc tggtgacgct ggccgtgctg ttacgtcgct
    14461 tctacccgca ggcgggcggt gcagacagta cgttgctgcg caccacgctg attgtcgggc
    14521 cgggcgcggt gatcctcggt ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct
    14581 tctggaagct gatgttccac tacagtttta cctcggtgat gtcgatgggg gtgatgctgt
    14641 ttcagctcta catggtggtg ctggtgctgt ggctggcgaa aatctttgaa tatgatttgc
    14701 ttgccctgca acaacgctgg ttgccgaagc tggggatcgt gcaaaaggtt ctgagccttc
    14761 tgacgcccgt tcatcgcgga ctggaaacgc tgatgctggt gttggcggtg ctgctggggg
    14821 cttataccgg ctttctgctg tcggcgctga aatcgtatcc gttcctcaat aacccgagcc
    14881 tgccggtgct gctcctcttc tccggcatct cgtccggtgc ggcggtggcg ctgatcgcca
    14941 tggcgatacg ccaacgcagt aacccgcatt ccacggaagc gcactttgtt caccggatgg
    15001 aaatccccgt ggtatggggt gaaatcttcc tgctggtggc gttttttgtc ggtctggcgc
    15061 tgggcgatga cggtaaagtt cgtgcgctgg tggcggcatt aggtggcggt ttctggacgt
    15121 ggtggttctg gcttggtgtc gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt
    15181 gggtcaatcg cagttccggc attcctgccg tgctggcggc gtgtggggcc agtctggtcg
    15241 gcgtgttgat gctgcgcttt ttcattctct acgccggaca gttgacggtg gcgtaagcca
    15301 gaaaagaggt ggtttctgga cgtattcctt cctgaagtcg gttttctggc gttgttgtta
    15361 agtctcgggg tcaacgtgtt gaccccgttg acggcctttg cgggagtgcg gttgcgctgg
    15421 cctgccatga tgcgactcac ttgcatcggc attctggcgc agttcgcgct cctgctgctc
    15481 gcctttggcg tactgacgta ttgttttctc atcagcgatt tctcggtcat ttatgtcgcc
    15541 caacatagct acagcatgct gtcgtgggaa ctcaaactgg cggcggtgtg gggcggtcat
    15601 gaaggttcgc tgctgctttg ggtgctgctg ctttccgcct ggagcgcgct gtttgcctgg
    15661 cattatcggc agcaaagcga tccgctattt ccgctgacgc tagccgtttt atctctcatg
    15721 ctcgccgcac tgctactgtt tgtggtgctg tggtccgatc ccttcgtgcg gatatttcca
    15781 ccagcaatcg aaggccgcga tctcaatccg atgctgcaac atcccggtct tatctttcat
    15841 ccaccgctgc tttaccttgg ctatggcggt ttgatggtag cggcgagcgt ggcgctggcg
    15901 agtctactgc gcggcgagtt tgatgcggcc agcgcccgaa tttgctggcg ctgggcgtta
    15961 cctggctgga gtgcattaac ggcggggatc atcctcggtt cctggtgggc ctactgcgaa
    16021 ctcggctggg gcggctggtg gttctgggat ccggtggaaa acgcctcttt attaccctgg
    16081 ctttctgcca ctgcgctgct gcacagtttg tccctgacac gccagcgggg gattttccgc
    16141 cactggtcgc tgttgctggc gatagttact ctgatgctgt cgctgctggg caccttaatt
    16201 gtccgttctg gcattctggt ttcggttcat gcgttcgcac tggataacgt ccgcgccgtg
    16261 ccgttgttca gcctgtttgc actgattagc cttgcgtctt tggctctgta tggctggcga
    16321 gcgcgggacg gtggcccggc ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc
    16381 gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg
    16441 atgatttacg gcctgctggg ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc
    16501 gcgacgttac cgtttggtct gttgatgctg gtggtgattg tgctggcgac gtttgtctct
    16561 ggcaaacgcg tgcagcttcc ggcgctggta gctcatgctg gcgtgctgtt atttgccgcg
    16621 gggatcgtgg tttccagtgt cagtcgtcag gagatcagcc tgaatttaca gccgggtcag
    16681 caggtgacgc tggcaggata caccttccgt tttgagcgac tcgatctgca agccagaggc
    16741 aattacacca gcgaaaaagc gatagtggcg ctgtttgacc atcagcaacg cattggtgaa
    16801 ttaacgccgg agcggcgttt ttatgaagca cgccgtcagc aaatgatgga accgtcaatt
    16861 cgctggaacg gcatccatga ctggtatgcg gtcatgggtg aaaaaaccag agcggatcgt
    16921 tacgcttttc gtttgtatgt acaaagcggt gtgcgctgga tctggggggg aggattgttg
    16981 atgattgcgg gcgcattgtt aagcggatgg cgggggaaga agcgcgatga ataaagggct
    17041 tctcacgcta ctgctgttat ttacctgttt tgcgcgcgct caggttgtag acacctggca
    17101 attcgccaat ccgcaacaac agcaacaggc gttaaatatt gccagccagt tacgttgtcc
    17161 gcagtgccag aatcaaaact tactcgaatc caatgcgccg gtggcggtca gtatgcgcca
    17221 tcaggtttat agcatggtgt cggaggggaa gaacgaagtc gaaatcattg gctggatgac
    17281 cgaacgctac ggagattttg ttcgctataa cccaccgtta acgggtcaga cgctggtgtt
    17341 gtgggcgctg ccagtggtgt tgttattgct gatggcactg atcctctggc gagtgagggc
    17401 taagcgatga aacagcctca aataccggtg aaaatgctga caacactcac gatattgatg
    17461 gtattcctct gtgtcggcag ttatctgtta agcccaaaat ggcaggcggt acgtgcggag
    17521 tatcagcgtc agcgcgatcc gctacatcag tttgccagcc agcaaacccc ggaagcgcag
    17581 cttcaggcat tgcaggataa aatccgcgct aatccacaaa acagcgaaca atgggcgtta
    17641 ctgggcgagt actatctgtg gcaaaacgat tacagcaatt cgctgctggc gtatcgtcag
    17701 gcgttgcaac tacgtggtga gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat
    17761 taccaggcca gccagcatat gaccgcccag actcgcgcaa tgatcgacaa agccctcgcg
    17821 ctggactcta acgaaatcac cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg
    17881 aattacgcgc aagccatcga actatggcaa aaagtgatgg atctcaactc accccggatc
    17941 aaccggacac agctggtcga gtcgattaat atggcaaaac tgttgcagcg gagatcggat
    18001 taatcatctg ttgatgaaga ttatgtcatt gagctgcata aaaaataatc gaatgaacat
    18061 atgccaaaaa taatcaccaa tcagtattat tgcagattaa acaaataaaa atctttccat
    18121 aacaaatggt tattcattaa tcctgccata aactcgcgtt attatgcatt aatgcagcga
    18181 aaagctctgt tgttaaaggg ttgcgcaaca taccgcgcaa atgatactga tcataagcgt
    18241 taaaaaaatc tacaaaccaa cgcaacacaa ttcatgccct ggcagtatgt cacgttctcg
    18301 cgtttctgaa cggggaacgg cgctccattg aggaagttat tctgatgaaa aatataaaat
    18361 tcagcctggc ctggcagatt ctgtttgcta tggtgctggg cattctcctg ggaagctacc
    18421 tgcactacca tagcgacagc cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg
    18481 atatcttcat ccatctgatc aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg
    18541 tgggtatcgc gggtgttggt gatgccaaac agctcgggcg tattggcgcg aaaaccatta
    18601 tttacttcga ggtgatcacc accgtcgcca tcattttggg gatcactctg gcgaacgtct
    18661 tccagcccgg tgccggggtg gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc
    18721 agagcactac ggaagcggta caaagcagtt cccacggcat tatgggcacg attttgtcgc
    18781 tggtgccgac gaacattgtg gcgtcgatgg cgaaaggcga aatgctgccg atcatctttt
    18841 tctcggtgct gtttggtctg gggctttctt ccctgcccgc gacgcatcgt gaaccgctgg
    18901 tgaccgtgtt ccgctccatc tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt
    18961 atgcaccggt gggtgtgttt gcgctgattg cggtgacggt ggctaacttt ggtttctcgt
    19021 ctctgtggcc actggcgaaa ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc
    19081 tggtagtgct gggaattgtg gcacgtctgt gcgggttaag cgtctggagc ctgattcgta
    19141 ttctgaaaga tgagctgatt ctggcgtact ccactgccag ctctgaaagc gtactgccgc
    19201 gaattattga gaagatggaa gcctacggag caccggcgtc gatcaccagc ttcgtggtgc
    19261 cgaccggtta ctcttttaac cttgatggtt cgacgctgta tcaaagtatt gccgctattt
    19321 tcatcgctca gctgtatggc attgacctgt ccatctggca ggaaatcatt ctggtgctga
    19381 cgctgatggt gacctcgaaa gggattgctg gcgtggctgg cgtgtcgttt gtggtgttgc
    19441 tggcaacgct gggtagcgta ggtattccgc tggaaggtct ggcgtttatt gctggtgttg
    19501 accgtatcct cgacatggcg cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc
    19561 tggtgattgc caagtgggaa cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg
    19621 aagtgctggg caaatttgat aaaactgcgg atcaataatt gaagattgcc ggggatatcc
    19681 cccggcaatg tgtggatgcc tgatgcgacg cttgccgcgt cttatcaggc ctaccttaac
    19741 cgttggttaa tttttcaaac tcttcacacc cggtatcaaa cccttccata cagctcaggt
    19801 tcaaccagtg cagcgccttt tgtttgttct tctcgataaa accttcctca ccgtttaaga
    19861 acatcattcc cgcccagtac tcggaataac cggttcggga aattgcagag ctgcgtttga
    19921 aataccaggt tgccttgtca tcgtcggcct taatgcccac gccgttggcg taaatcaaac
    19981 caagcagcat ttgggcatcg acggcggagt cattctccag atcttccgag gcgttttcta
    20041 acagcgaaat cgcctttgga taatccggtt tacccgcttg agtatttacc agaatatgcg
    20101 ccagcgttac ttcaccttct ttactgcccg cttgcgccgc tttctctgca agaacttttg
    20161 cttgtggata gtccagactg accggattgg tgattttgat ctgcgccaac agcgcgcagg
    20221 catcagcatc accgctgtcg gcggctttct gtgcccaata ttcggctttg ctcaaatcgc
    20281 cggagctaaa ccagctgtcg gcaagaaaat attgtgcgcg ttggtccccg gcctctgctg
    20341 cctttaaata ctggctgcca ggctcggagt cgttcgcgtg ggcaaagaat gtcaaaaaca
    20401 acatcaatgc gataattttt ttcattttga taatacgatt gggtagggag taaccagtat
    20461 aaagagttcg aatggataaa aaaacagcct ccgaaaggag gctgtagaaa ggactgtatt
    20521 acgccagtgc cgcttcgcgc aggcgagttt tcaacttgtt gtactcgtca atcaggtact
    20581 gctcggcggc gcgctgatcg gagatcggct cgacgcgcac ggcgcagtat ttgtattccg
    20641 gcgttttcgt aatcgggctt aagttttcgg taaccagctc gttacaggca ccaatccacc
    20701 actggtaggt catgtaaatc gcccctttgt tcggacgatc gctgacctgc gcacgggtga
    20761 taattttgcc tttacgcgag tgcactcaaa ccaatgcctc atcttcaata cccagacgtt
    20821 tggcgtcttc ggtattgatt tgtgcgtagc caggttcatc agccagcgcc gccagtgccg
    20881 cacagttacc ggtcatcgaa cggcaagagt agtgaccaac ttcacgcacc gttgacagta
    20941 ccatcgggta ctcgtcggtg agtttgtcga ttggcgctac ccagtcgcag gtgaagaact
    21001 gcgccagacc gttcggggta tcaaacttct ctttaaacag ataagaagtc ccctgatcgg
    21061 catctgaagt atcgcggcaa ggccactgaa tgaagcccag ttcgcccatt ttctcgtaag
    21121 tcgcaccgta gaaatccggg cacagatgac gcaactcatc ccagatctcc tgggtgttgt
    21181 tgtagtgcat cggataaccc atacgggtgg cgatttcact gatgatttgc cagtccgttt
    21241 tcagatccca tttcggttca actgctttga agaaacgctg gaagccacgg tcagccgcag
    21301 taaacacgcc ttcatgctcg ccccacgacg ttgacggcaa aatgacatcc gccgccgacg
    21361 cggttttggt cataaagatg tcctgaacga tgaccagttc cagatcttca aaggctttac
    21421 gtactgccga cagctccgca tcagtttgca gcggatcttc gcccataatg tacgcggcac
    21481 gcacttcgcc atgcgctgcg cggtgcggca gctcgctgat gcgatagccg gtatgagcag
    21541 gcaggctttc cacgccccag gctttggcga atttctcgcg gttagccgga tctttcacgt
    21601 actggtatcc cggatacgta tccggcagcg cgcccatatc gcaggcaccc tgcacgttgt
    21661 tctgaccacg aaccgggttt acacccgcat gcggcttacc gaggttaccg gtcagcatcg
    21721 cgaggctggt cagagaacgc acggtttcca cgccctggta gaactgggtt acacccatgc
    21781 cccacaggat ggcggcgctt ttcgcctggg catacatccg tgccgcctga cgaatctcac
    21841 tggcgctgac accggtgata tcttcaaccg actccggcgt gtagccttca acgattttac
    21901 gatactcttc aaagccttct gtacgggaag cgacgaacgc tttgtcgtac agattttctt
    21961 caataatgac atggcccatc gcattcaaca gcgcgatgtt cgagccgttt ttcagtgcaa
    22021 tgtgcatgtc agcaatgcgc gcggtttcaa ttttgcgcgg atcgcagacg ataattttcg
    22081 ccccgttacg tttagcgtta attacgtgat tcgccacgat tgggtgggaa tccgccgggt
    22141 tgtacccgaa aacgaacact aaatcggtat tatcaatttc gttaatagca ttgctcattg
    22201 cgccattacc gaccgattgg tgcagacctg caaccgatgg gccgtgtcag acacgagcgc
    22261 agcagtcaac gttattggta ccaataacgg cgcgcgcaaa tttttgcatt acatagttgg
    22321 tttcgttacc cgtaccacgc gaggagccgg tcgtctggat ggcatccgga ccgtacttct
    22381 ctttgatggc gctcaggcgc tcggcaacgt aattcagtgc ctcatctcag gaaacaggtt
    22441 cgagtttgcc gccacgctgg cgacggatca tgggggtttt caggcgcggg gtcaggatct
    22501 gggtatcgtt aatgaaatcc cagccataat aacccttcag acacagggta ccctggttgg
    22561 ttttcccctg cgctgcctcc gcccggacga ttttgccgtt atcgacgacc aggttgattt
    22621 tgcaacctga tgcgcaatag gggcaaaccg tgacgacttt tttcatcggt ctcgctccag
    22681 ttaatcaaat cacgcatacg cgctctcgac tacagtatgc atcttttatg ccacatttta
    22741 tttggggtca ttccctgata ttacgggcac tatttattca aaactctgac gaaaaacagg
    22801 ctgtcgtcag ttttgacgtg acgaaacgaa ataccgcgtg acagccatca cgcggcagac
    22861 attttatttt ttctcgacga cgggagccgc ctgatacccg ccgcccagcg atttcatcaa
    22921 ctgaatgctt tggatcaccc ggcggctgtc cagcatcagt aatgacatct cttcggcaag
    22981 tactggcaac cgggcttcag tggcctgtaa gcggctggtt aagccgcgct gataggcggc
    23041 ctcggcagcg cgctgggtga agcgcgttgc ttccacgtgt tcagcctgca tttctcgctc
    23101 gtcgttgagc gtttgcagac gcgtgccgtt gacggcaacg tcacgcaccg cgttcagtac
    23161 tgactggttg taacgttcaa tcatcatgtt gctggcggcg cgcgtgcctt cgagattggc
    23221 attcaaccgt ccaccgtcaa acagcggcaa tttcagaccc gggatgaagt tgaactggcg
    23281 actggttttt ttgaataagg tatctaggtg gatggcatca aggccgaaaa acgctttgat
    23341 atcaaagctc ggatagaaca acgcccgcgc ggaatccacc tgatctaatg acgcctgaac
    23401 ataccagcgc atggcttgca gatccgggcg tctggcgagc aactcataag agagtgtcgc
    23461 cggaatgccg gtctggactc gtggtaatgc cacaggtttg atctccggca tatcgctggc
    23521 tcccgcgcca atcaatgcgc gcagagattc tctcgtttcg gtgatttgcc ctttgacggc
    23581 agcaatttgt ttatcgaccg ccagaatctg tgcccgcgcg ccgtggaaag gcacttgtgc
    23641 ttccagaccg tgcgccactt tactttggtg cgctttcacc gcgtaatcaa tcacatcgcg
    23701 agtttgttct aacagatcga gcatctgata gctggcctgc atactgtaat aaagctgcgc
    23761 tacgcccgtg gtcagcgata gctctactgc tgcggtttct gccagcgcgg cattatgcgc
    23821 gccaatggcg gcggcaaccg ctgagcgatg cacaccccac aaatcaagat ccagtccggc
    23881 aaacaaacct actgtggctt ccgtatagta cggcccgtcc atccccagtg cgggcgcatc
    23941 catcgcataa gggcttaaaa agccgttcgc cgagacacgt tggcggttaa gcatccctaa
    24001 cgctgccacc tgcaactgtg aaccggcatc taacaaatcg gcctgcgatt gcgctttttc
    24061 ttcccgcagt ttcgcttcgg cgagggtgtg tgaaccactt agcgtccgtt ggatcagggc
    24121 atccaactgc gggtcattga gttgtttcca ccactgcgcc tgcggccagc cggagctggc
    24181 aagatgaata tcatcggcca gtttgatttg ttccggtttg agctgttgat gaggcgcaga
    24241 atccttacgt accagggcac agccggaaat cagcgtcgtg ctgccgagaa tgctgcacaa
    24301 cagcagacgt gaaagttgac gattgatcat tgcgtggctc cttgtgcctg ttccgtggcg
    24361 ggcgttaatg ccggggctgt ccagtctggc aagcgggcga aaagctggca gacgtgttgc
    24421 tcctgtttta ataaggtggg caggatggcc tgcgaggcgg gtgtctcttc aagcgttatt
    24481 tgaggagaac ggctgagtgc ggttgccaga ccggcagcgt atttctccag ggcgtcggca
    24541 aactgacctg ctctgtccgg ctgtagtgcg ttatccagcg cctgcgccga attccaggtg
    24601 gcatcccagg cgtgaaggat atcgcggccc tgacgaataa ccgtttgcga acgttcaatc
    24661 agtaaggcgc gttcttcgct gtccagttga cgctccagcg ccacgcgttg gcacatctct
    24721 tcacaggcat taaacgccgc atgcagaccg atacgaattt gcagataagt gcgcagagcc
    24781 gtgacttcct gctggcgtgg gatccgcatt actttactta acatacccag cgcgccagcc
    24841 agtttttgcg gcagcgtgcg cgcttcactt tcaggccaga caaaggtgta aatcatcgcg
    24901 gacaccacgg taccaatgat gatacccagg gcgcgatcgc gaatttccac caggtcgtac
    24961 actgggccaa aaacgttttc gagcgtggcg agcgcgaagg tgaccaccat ctgtgtgccg
    25021 atataagaag agcgttcaga gctggtggca atctatgcgc ccaacaggaa aatcggtgcc
    25081 agcacaaaca gcaattcgac aatattgtcc agccagggca tgaccagtag cgtgaataac
    25141 agcgccagaa tcgcgccgca aaaggccccg ccaaaacgca gcaccatctt ctggtacgac
    25201 gaaccgacat ttggattagc gacgatcacg caggtcagca tacaggtgtg aatgccttcc
    25261 cagtccacgc cgctgtaaaa ggtgtagcag atcaaacagg cgagcaatgt ttttaccgcg
    25321 tagcgtatat agtctggatt ggtaaaagca tcggcgacca ttgatggcgg tttggcggcg
    25381 ggcgttggcg gcgtattcgg gttcatctgg cccagttgta acaacgtctg gcagatattc
    25441 tccaggttac attcccgtgc cgccaccgct tcactttcac tgatccgcca gtcgctttgc
    25501 cagcactgac cttcagcaac ggcatgctgc agcttgttga tttctgaggc taatttttgc
    25561 cgaaattcaa taattgcctg agaatcagca aaagaggtgg gatcgtagcg attcagcgtc
    25621 gagtaaatgt aggttaccgt tgccacgcag ctttgccacc atgcgctttg agttcgccag
    25681 ttggcatcgt ccgcgagaca aaagacattc agtttttgca gcgccagcgc ctctctttca
    25741 atccgcgttt cgggtagcgg tgcgaggctg tccgtcaggt ggctaatggc atcatcaagc
    25801 agatcattaa gcgcttgatg catttgcgta atggcacgat tgggaaacca cagcacgccg
    25861 attaacgtca tcagcaaggt tggatagagg ccaacaacga tacaccacag cgttaagcgc
    25921 acgactactt ccggatagtc gagcatggcg gggaaggttt gcccgtaaat ggcgacaatg
    25981 gcgacagcga aaaagaccag tcccagccga tgggtgcgca tcaaaaacat gcagcccatc
    26041 aggatcggcc cggcgatgat caatcggatc aacggttcgc cgtatgacca tttgtagatc
    26101 aaaaacaggc tgccgatctc cagcaccgtg gcaaccacaa acaagatcgc cacaaatttg
    26161 gtgtaaaacg cgttcgactg aataccgtaa aacagcactg ccagcgataa cgccacaaaa
    26221 gggatctcaa aagtcatcga gatcagaatc accagcaggc agcctaccca gagttgcacg
    26281 atctgcggca cgcggccggg tcgccgctcg cttaactctt catgaaagaa cgccagcagc
    26341 ctgaccaccg gtaatggcag ggagttgagc gcgctcatta ttgcggctca agattagcga
    26401 ctgccgaagc gccgatgcgg aacatttccg ggtcaggttt atcgaccatg attttgaccg
    26461 gaaaacgctg ggcaacgcgg acccagttaa tagaacgaga cacttttggc aggccgccca
    26521 gcaccaggcc gccgtcatcc ggtagcacgc cgtagccaat cgaatccact ttaccctcga
    26581 aggttttgcc gctgtcactc atcaggcgaa tcgttgcggg tgtacctgag cgaatatttt
    26641 tcagatcggt ttcgcggaag ttggcgatca cgtaccagtg acgagtgtcg atgagggtaa
    26701 aaatagggcg catggcagaa gcaaattgcc cgacggaggt tttgagggaa atgatccggc
    26761 catcaaatgg cgcgcgaacg gtcgtcattt ccagatgtag tttggtcagg gcaatatccg
    26821 cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc ttctgctgag acaaaaccct
    26881 ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc ctgtttcgcg g
//
LOCUS       DAJTYI010000044        25973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYI010000044 DAJTYI010000000
VERSION     DAJTYI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            96..1079
                     /locus_tag="OO564_001816"
     CDS             96..1079
                     /locus_tag="OO564_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6652964.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1102..2613
                     /locus_tag="OO564_001817"
     CDS             1102..2613
                     /locus_tag="OO564_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6652965.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2638..3636
                     /locus_tag="OO564_001818"
     CDS             2638..3636
                     /locus_tag="OO564_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6652966.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3669..4763
                     /locus_tag="OO564_001819"
     CDS             3669..4763
                     /locus_tag="OO564_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6652967.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4775..5647
                     /locus_tag="OO564_001820"
     CDS             4775..5647
                     /locus_tag="OO564_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6652968.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5695..6117)
                     /locus_tag="OO564_001821"
     CDS             complement(5695..6117)
                     /locus_tag="OO564_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6652969.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6214..7410)
                     /locus_tag="OO564_001822"
                     /pseudo
     CDS             complement(6214..7410)
                     /locus_tag="OO564_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7420..8232)
                     /gene="hcaB"
                     /locus_tag="OO564_001823"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7420..8232)
                     /gene="hcaB"
                     /locus_tag="OO564_001823"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6652970.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8229..8549)
                     /locus_tag="OO564_001824"
     CDS             complement(8229..8549)
                     /locus_tag="OO564_001824"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6652971.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8549..9067)
                     /locus_tag="OO564_001825"
     CDS             complement(8549..9067)
                     /locus_tag="OO564_001825"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6652972.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9064..10425)
                     /locus_tag="OO564_001826"
     CDS             complement(9064..10425)
                     /locus_tag="OO564_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6652973.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10561..11451
                     /gene="hcaR"
                     /locus_tag="OO564_001827"
     CDS             10561..11451
                     /gene="hcaR"
                     /locus_tag="OO564_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6652974.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11611..12750
                     /locus_tag="OO564_001828"
     CDS             11611..12750
                     /locus_tag="OO564_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6652975.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12742..14022)
                     /gene="csiE"
                     /locus_tag="OO564_001829"
     CDS             complement(12742..14022)
                     /gene="csiE"
                     /locus_tag="OO564_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6652976.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14213..15067)
                     /locus_tag="OO564_001830"
     CDS             complement(14213..15067)
                     /locus_tag="OO564_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6652977.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15212..16015)
                     /gene="suhB"
                     /locus_tag="OO564_001831"
     CDS             complement(15212..16015)
                     /gene="suhB"
                     /locus_tag="OO564_001831"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6652978.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16134..16874
                     /gene="trmJ"
                     /locus_tag="OO564_001832"
     CDS             16134..16874
                     /gene="trmJ"
                     /locus_tag="OO564_001832"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6652979.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17144..17632
                     /gene="iscR"
                     /locus_tag="OO564_001833"
     CDS             17144..17632
                     /gene="iscR"
                     /locus_tag="OO564_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6652980.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17744..18958
                     /gene="iscS"
                     /locus_tag="OO564_001834"
     CDS             17744..18958
                     /gene="iscS"
                     /locus_tag="OO564_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6652981.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            18986..19372
                     /gene="iscU"
                     /locus_tag="OO564_001835"
     CDS             18986..19372
                     /gene="iscU"
                     /locus_tag="OO564_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6652982.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19389..19712
                     /gene="iscA"
                     /locus_tag="OO564_001836"
     CDS             19389..19712
                     /gene="iscA"
                     /locus_tag="OO564_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6652983.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19808..20323
                     /gene="hscB"
                     /locus_tag="OO564_001837"
     CDS             19808..20323
                     /gene="hscB"
                     /locus_tag="OO564_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6652984.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20340..22190
                     /gene="hscA"
                     /locus_tag="OO564_001838"
     CDS             20340..22190
                     /gene="hscA"
                     /locus_tag="OO564_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6652985.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22192..22527
                     /gene="fdx"
                     /locus_tag="OO564_001839"
     CDS             22192..22527
                     /gene="fdx"
                     /locus_tag="OO564_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6652986.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22539..22739
                     /gene="iscX"
                     /locus_tag="OO564_001840"
     CDS             22539..22739
                     /gene="iscX"
                     /locus_tag="OO564_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6652987.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22798..24081
                     /gene="pepB"
                     /locus_tag="OO564_001841"
     CDS             22798..24081
                     /gene="pepB"
                     /locus_tag="OO564_001841"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6652988.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24223..24999
                     /gene="sseB"
                     /locus_tag="OO564_001842"
     CDS             24223..24999
                     /gene="sseB"
                     /locus_tag="OO564_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6652989.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25494..>25973)
                     /gene="sseA"
                     /locus_tag="OO564_001843"
     CDS             complement(25494..>25973)
                     /gene="sseA"
                     /locus_tag="OO564_001843"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6652990.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6142 a         6497 c         7212 g         6122 t
ORIGIN      
        1 ccctattaac tgtaatattt gagcggcacg cgctgccgct catcacaaaa taattagaat
       61 aatccgttgt taataaaaac cctacaggag gcactatgcc taaaaaaatg agaacaaccc
      121 gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg gctgcggata
      181 aagaaatgac cattggtgca atataccttg atacccaggg atattacgct ggagtgcgtc
      241 agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt gaaactaacg
      301 cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg cgtaatgtcg
      361 atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt cgtcgcgcca
      421 gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag ggtgtcgata
      481 aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa ctgggtaacg
      541 ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc gtcatcaatt
      601 gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta ttaaaatccc
      661 gcgttcccgg cgcgcaaatt gtcgctaacc aagaagggac tgttttagat aaagcgattt
      721 ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg ggggagtcgg
      781 gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc ggaaaaattg
      841 ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac aatcaggtgc
      901 tgaaagcggt agtggatatt tccggtaaga aaatgggcaa tgctgttttc gcgcaaacat
      961 tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca atcgatctct
     1021 ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt ctgccctaat
     1081 cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt agcaaaagtg
     1141 gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa cttcacgctc
     1201 aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc gactctcatt
     1261 cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg cgagacgcga
     1321 ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt tcgtgcggtt
     1381 tatcaggaat taagtctggt ggaagggctg acggtggcag aaaatctctg ccttggtcaa
     1441 tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc ccaacgttgc
     1501 ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct aagcccggcg
     1561 caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt ggtcattctt
     1621 gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag tgcggtgaaa
     1681 aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga agaaatccgc
     1741 cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga tgtgatgctc
     1801 gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca cgttgatatt
     1861 gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg tgcgttacgc
     1921 cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt gcttggcatt
     1981 gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg gctggagacg
     2041 tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga ttacggcgac
     2101 atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg gatcattccc
     2161 tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag caccaacggt
     2221 gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg gatgacggtc
     2281 aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca gcaaaaagtg
     2341 gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga gccaacgcgc
     2401 ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct ggctgccgaa
     2461 ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt gtgtgaccgc
     2521 atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt caatgtggat
     2581 gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa cctgaagatg
     2641 tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca atttgtcagt
     2701 cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct ggtcttctcc
     2761 ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg cgatgccgcc
     2821 accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga aattgatgtc
     2881 agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct gcaatttgaa
     2941 gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat gggaacgctc
     3001 gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact ggggctgtgg
     3061 agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc gattgacgaa
     3121 aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc cgcgctgatc
     3181 atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt cgggcgctcg
     3241 gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa cgttcgtcga
     3301 gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg cattttgttg
     3361 gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt tgacgtcatc
     3421 gccgcggtcg tggtcggcgg tacggcactt tccggcggtc gcggttccct gttcggtaca
     3481 ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct cggtattaac
     3541 tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct ggcgaatatc
     3601 ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct ggcgcgggtg
     3661 cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg atgctggcag
     3721 cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg acgccgggga
     3781 ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat gtccactata
     3841 tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac cagggattta
     3901 tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctac cgccatttta
     3961 aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc ccgaactgcc
     4021 gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc tggcagcgtg
     4081 acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc ctgccggatg
     4141 cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc tatgaaggga
     4201 ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg ggggcggtcg
     4261 gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc ggcgttgata
     4321 tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac ggctatttag
     4381 caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc gcggatgttg
     4441 cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc actgctgact
     4501 gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc agcgctgatc
     4561 tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc catatggaaa
     4621 aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt acccatcgct
     4681 tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa tgcgggaaag
     4741 ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct tatcccacgg
     4801 gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct ggcgcgatac
     4861 gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct catgttttcc
     4921 gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg ggcgtgagta
     4981 tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg gctggctggg
     5041 cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg aacatcgcag
     5101 cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata cgctgacggt
     5161 gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg gctggcatcc
     5221 ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt actggctgga
     5281 acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac tggattttaa
     5341 ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat ggaatggtca
     5401 ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga cgccacctgc
     5461 accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg cgtttgattt
     5521 cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg aagggggtga
     5581 tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc gggtagagtg
     5641 gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc tttcctaacg
     5701 ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg cgccagcgat
     5761 actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg tcatatccca
     5821 gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca ccgccagcgg
     5881 acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa ctaccgcaat
     5941 aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga ccgtaccgtc
     6001 aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg cgatacgggc
     6061 aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg tgttcataac
     6121 caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac ctcgccacgc
     6181 cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg cgatgttctc
     6241 atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc gaattgggcg
     6301 aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt gcagattaaa
     6361 ccaaatcgcc ttctgagttt ctgggttgcc acgacaaagc tagtcatcgc cacgcatatc
     6421 gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg gcggcagtag
     6481 cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggcgt tttcccagct
     6541 ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc caccggcaaa
     6601 gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg cagtatcaag
     6661 gttggcctcg cgagccagtt gctcgttggc gctgatacta ataccgtaaa tcaccacatc
     6721 agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat cgaccacatg
     6781 ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct gtaaaagata
     6841 gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt caatcactgt
     6901 caccttacat ctgcgctgcg tggcgctggc agccagttcc agaccaatag ttccggcacc
     6961 gacaatcacg actgaccgtt cgggctgcag aacttctcgc agtctggcgg catcaccggc
     7021 atggtgtagg gtaaagcagc gttctcccag tgcatcaagc aacggcagcg gtcgagctgc
     7081 cgcgccggtt gctataaaaa gctgatccca gtgccagctt tcgccgttgg ttaacactaa
     7141 ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg gaatgcagat gaacattgtt
     7201 ttcctgccac cagttagcgg gtaacacctg ctgtaactgc ggggaatctt ccagcaacat
     7261 ggatttcgag agtggaggtc gttcataagg aagatgtcgc tcatcggaaa acagatgcag
     7321 ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc attgccgccg cttgcccgcc
     7381 accgacaata atgatcgttt tttctttcat cgtgcttcct taaagatcca gcccagccgc
     7441 tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg ttgatcatca caccgcttaa
     7501 tgcgcgatta ttgcgccgcg atgccaacat cacatacggc cccgtaaaat ccgccggttg
     7561 cgggaaaaat tgcagcggta aaatggcggc aattttctcc ggcgtcagag actgcattat
     7621 cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg tcgctggcca taccacacgg
     7681 gccgacgcca ttcacccgca ctttcggtgc cagttcataa gccagttggc gaataagtcc
     7741 ggttgcggca tgtttactgg tggtgtacag cgggccaccg ccgccaggat accaggcggc
     7801 atttgacagt gtgaaaatca tgctgccttc actggcgatt aacgccggag cgcaggcttt
     7861 tgcgcccagc aggtaaccga gtacgttgac gttaaacagc tcgtggaagc cggtttcgag
     7921 cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc cagatgcctg cattgccgat
     7981 aaaacaatcc agcttgccgg aacgagtcag gatctgatcg accgcgcgtt gataatcggc
     8041 ataacaggtc acgttacctt ccaccgccag aatatgttcg ccaaatcgct gacgcagact
     8101 ggcgactttt gccgccgaca gttccagcgc ggcaacctgc gcgccttctt cgataaatcg
     8161 ctcgaccagc gccagcccta atcccgatcc gccgccggta ataaaaatgg actcgttatg
     8221 cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa tgtcaccacc ttcaacgtgt
     8281 actggataag tggtgagcgg atcggtggcg ggcaggcata acgctttccc cgttttcagg
     8341 caaaaactgg cggcgtgtag cgggcactcc accgtggcgt catcttccag atacccttct
     8401 gacatcgacg cattaccatg gctgcaacga tcgttaatgg cataaaactc gccgcccacg
     8461 ttgaacaagg cgatgacggg cgaggtatct atccggagag cctcaccctc cggcacatcc
     8521 gcaacgggac acgcataaat tcgattcatc agaacagtac actcaggtta tgggaagtga
     8581 tcaccgcttg atccaggacg atatcccgtt ccagcaagcg ccagttgtca tcttccagac
     8641 ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc atcgcgctct ttttgtgccc
     8701 gataaagtag ataattcacc ctcacggcaa atacgtttgg gatgtcggtt tcgcttacct
     8761 ggcagttgct gattaagtga cgagtgcgtg acggcggctc ttctgcccag gccatgcccg
     8821 tttccagacg ggcgattcgc cgctccagct ggtctttggt gtcattgaaa atccaggtcg
     8881 tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc gttaaccgtg gtgcgcatgg
     8941 tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg aaatttccag tcatccagca
     9001 gactggcttc gtgaaagaga aactggctaa tgcggtgatg taactccagt gaaacttgcg
     9061 cactcatttc atcacctcct gctggtaagc ggcggtttta tcgagcactt cctgccagct
     9121 ttcgctactc agaagatcgg cccagcgttg gtacattcca cgagcggcag tttctgaaaa
     9181 gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc ttttcctgac caagccccat
     9241 ttccagacac agtttgctgt tgcgggcgcg gtggccttta agcaatttct ggatttcaca
     9301 ccagttctcc gagtcatcct gctcgagaaa accagcagga ccaaaagcac gagtggcgct
     9361 gttttcaaaa gcggctttaa cttcatcgga ggcggctttg tcagtaatac agaacgccca
     9421 cacttcaact tgatcagggc cgcgcggatg ccagacgcgg agtgtggcag tgccgtttag
     9481 ccatgaaagc gtggggaata tattgttatg acccgccagg cgcagggcgc gaacttcacc
     9541 taaacgttgt tctgcttcgg cataggtttc gcggtaatag cttgaaactg cgccatcgac
     9601 ccagacatta gcatccggtt tttcagtaaa gaagaaaccg ctaccgtgac cgtcctgacc
     9661 aaattgcagc gcatctttgg cggtttccca caccgggcgg gcggtttgtc catcaccgag
     9721 gcgcttatcg ctgccatcat ctttcgcccc taatacctga acggcagaag catggctgaa
     9781 cagagcatga tactggtcac tggcgaactg ctctgccggg aatttccagt tacagttgat
     9841 cacccacttt tgtacgccgc cgacaatttc ggtgccgcct tcgcgacgat ccagcatgcc
     9901 atccagatac caggcaatgt cacccaggta atcatgcagg cccggtgcgc tggtatccca
     9961 gttgccaaaa atcagtcctt tataactctc cacacaagga acttcgttta gcccccagtg
    10021 ggatttacac aacccttgtg ggtaggcgcg aggttccagc ggtacatcga tcaactcgcc
    10081 gttaatgcca taagaccagc cgtgatacgg gcaggtaaag gcgcgagtgt tgccacaatc
    10141 tgcataactc acacgcatgg cccggtggcg gcattggtta agaaacgcct tgatgctgcc
    10201 gtctttctgg cgcaccacga caactgcatc ttctcccatg taggtgttaa agaaatcacc
    10261 gggttttggg atctggcttt cgtgggcgag aaatagccag caacgaccga aaatacgctc
    10321 aagctccagt tggtaaatgt ccgggtcggt ataaatacgc ggagtgaccc gaccgttttg
    10381 ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg gtcatatttt ttaatcctga
    10441 aagataagat gtattgacct tctatttttg tgccataagg atgttgctgg ttggttgcta
    10501 atatgtgaaa tagttaattt ctaccagctg agacgttttt tcgataacaa ggggaaggtg
    10561 atggaactac ggcatttacg ctatttcgtc gcagtggcgc aggcactgaa ctttacccgt
    10621 gcggcggaaa aactgcatac ctcacagcct tcgttaagca gccagatccg cgatcttgaa
    10681 aactgtgtcg gtgttccgtt actggtgagg gataagcgca aagtcgcgtt gacggcggcg
    10741 ggagaatgtt ttctccagga cgcgctggca atcctcgaac aagcggaaaa tgccaaatta
    10801 cgggcgcgga aaattgttca ggaagacaga caattaacca ttggctttgt gccatcggcg
    10861 gaagtgaatt tattgccaaa agtattaccg atgtttcgtc tcagacagcc agacaccttg
    10921 attgagctgg tgagtttaat caccacgcaa caggaggaaa aaattcgccg tggtgaactc
    10981 gacgtcggct taatgcgcca tcctgtttat agcccggaga ttgattatct ggagcttttt
    11041 gacgaaccat tagtggtggt gttaccggtt gatcaccctt tagcacatga aaaagagatc
    11101 accgccgcgc aactggatgg cgtgaatttc gtcagtaccg atccggcgta ttccggttcg
    11161 cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca gccagccaaa tatcgtccag
    11221 gtggcaacga atattctggt gaccatgaat ctggtgggca tggggctggg cgtcactttg
    11281 atacccggtt atatgaataa ttttaatacc gggcaggttg tttttcgtcc cattgccgga
    11341 aatgtgcctt ccattgcttt actgatggcg tggaagaaag gggagatgaa gccggcatta
    11401 cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg taacggcata attacgcatt
    11461 atttttgcaa catgcgccgc ggatcgcagt tttttagtgc ttgtgaactg gcgttacaga
    11521 catcgggaag caggctcgaa tattcttatt tttcaacttg caccaacatt cgattaaccc
    11581 cagactaacg cctcctgacg ggagggactc atggttttgc aatccacgcg ctggttggcg
    11641 ctcggctatt tcacatactt ttttagttac ggcatttttt tacctttctg gagcgtctgg
    11701 cttaaaggga ttggtttaac gccagaaacc atcggcctgt tattgggggc aggtctggtt
    11761 gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca gcgatccttc ccgcctgatt
    11821 tccgccttgc gcgtgctggc actgctgcca cttctctttg ctgtcgcctt ctgggcgggg
    11881 gcgcacgtag cgtggctgat gctggtgatg attggcttta acctcttttt ctcaccgctg
    11941 gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc agttcccgct tgattacggc
    12001 aaagtgcgac tgtggggctc ggtggcgttt gtcattggct cggcgctgac gggcaaactg
    12061 gtcactatgt ttgattatcg ggtgatcctc gtgctgttga cgttgggcgt ggcatccatg
    12121 ctgctcggct ttctcatccg tccgacgatt cagccacaag gggcaagccg ccagcaggag
    12181 agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga actggcgctt tctggcctgc
    12241 gtttgtttat tgcagggggc acatgcggcc tattacggtt ttagcgccat ttactggcag
    12301 gcagctggct actcggcctc ggcggtgggg tatttgtggt cgctgggcgt ggtggcggaa
    12361 gtcattatct ttgcgctgag taataaactt ttccgccgtt gtagtgctcg cgatatgctg
    12421 ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta tgggagcaac tacggcgttg
    12481 ccgtggttga tagtggtgca aattctgcat tgcggcacct tcacggtctg ccacctggcc
    12541 gccatgcgtt atattgctgc tcgccaggtt agcgaagtca tccgtttaca ggcggtttac
    12601 tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg ttttcgccgg tttcctgtat
    12661 caatatctgg gccacggtgt gttctgggta atggcgctgg tggcgcttcc ggcaatgttt
    12721 ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt ttgcggatct gctcctgttg
    12781 aggtgttgtc agcgtcaggt ccgtatagat cagcggtggt gaaaagagcg gtaacggcat
    12841 ggtataaggc gcaataatca gtgccgcgcc acgcggagcg cctgtctgca aaaatgcctt
    12901 taccgacata tgcttaatgt tgagcggcag taacgttagt tcgcgtaact gctgctcaat
    12961 ctgcgcttct cgctcgctat cattcccggt cagtagaata atctgttttt catgcagatc
    13021 gttttcctgc attagccagg caccgaaaat caccgcgacc agaccacttt cctcatcaga
    13081 aagatggacg ccgtattcac tttcaaatcc ggccagcgcc gcgcgggtga tgcgtaccag
    13141 gcgtggatac agtctggcga actcttccgg cagggtatta tcaataccga tggcaaacaa
    13201 actgcgattt aacgcctggg cgaggtggat ataaagctga tcacataacc cctgttcatc
    13261 ataaaaacga acatttccca gctcacgaaa atggtttacc agacgtttga tagactgacg
    13321 caattgtctg tcccgctgat gcgcatcccg cagtggatcg ggaacccgca acatggaaaa
    13381 aagtaatgcc ataaacagtg gctcatcagg cggtacatgc tggagcgccc gacgctgcca
    13441 gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag gattccgccc agcgacgttg
    13501 gagcggatta aactgaggcg taattccagc gtggtgctgc agcaaacaat attgcagata
    13561 cagacacagg aaatgaatat cgcgcgattc aaagcgtttt tgcagccgtc gggagcagag
    13621 attcactaac gcttgtagat tggtatcgtc gtaaaagtta cgcgcaattc cgcgccgctt
    13681 cagttcactc ttcagggcgg gggtgaattg gctggtaata aatgacggac acagacggaa
    13741 accacgtcgt agccagtgaa ataaacacaa acgttggtta agcactgtac cttcaacccg
    13801 gtagctaccg tcataaccag tcgtcagtgt gagttgatga tagcgcagga tctcccgtcc
    13861 tgtttcgctg atatcaagac tggcaatatc atcatccaca ccattaagct cgctgaaggt
    13921 tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc aagatctggc agcggcgctg
    13981 gggagccgaa aggacagatg gtggagcaag cgtaggcatc atctgtagat ctcccataag
    14041 cataattgat aagaatagct aaagtttgct cagggaaatg gcgaagggaa tgctaatcat
    14101 cagaaatgtt ggcaagcgtc acagaatttt tctcatttta aactgttctg accagtggat
    14161 tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct agcgatcatg ggttagttct
    14221 gcggattcaa cgcgctaagg ataaagtcca ccatctgttc gcgataaaca ttgccgtgac
    14281 gatcggaaaa agcatcaatg tgttcgccat ccgggattag gatcaaccgt tttggctctt
    14341 ttgccaggct atacaacttt tcgctgtgct gccatgggat aacgtgatcg gctttaccgt
    14401 gaatgagtaa aagcgggatc gggctgacgc tggcgatgta attttcgccg ctgtaactct
    14461 catcaagtaa gtagccactg ccggggagca tctggttggc gatggttgca taagaggcaa
    14521 atgtggagtc gaggatcacc gcacgtatgc cttcacgatc accctgacca ataacagcaa
    14581 gaatattcgc cccgccaata ctctgcccga acagcaccag ccgttgtggg tttacatcac
    14641 tgcgatggcg caccacattg atggcgcttt gcgtatcgtc cagcaatccg gcctgggacg
    14701 gagtgccttt tgatttacca aacccgcgat aatcaaacat aaaaacgttg aaattacgct
    14761 caggtaacca actgaccagc ggccagtggg cggacatatt tccggcattg ccgtgagcat
    14821 gaatgatggt tgcgatggcg ttgtcagcag ggcccgtcga agaaggaata aaccagcctt
    14881 gcagacgagt accgtcctta gccgtaaatt cgacggattc cgccgaccag gggtcgggac
    14941 cgtaaatttt atcgtcagga taatagaaga agttgatggc aacgcgcagc actaaataaa
    15001 cgcagaaggc aacaacaaag agaataaaca gaattttggg aacgcgtttg ttcactggca
    15061 gtgccataac ctttccttgc gacttcttta tcatccatta atcggggaaa gatggtggga
    15121 gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg aatagcgcct gaaaggcgag
    15181 ggcgggtgag tgatatcacc cgcctgagtc attaacgctt cagagcgtcg cttaactcgt
    15241 cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt accagcaacg atgttaccgg
    15301 tcagcatata gttatgacca ccggtgaagt cgctgacgat gccgcccgct tcacgaacca
    15361 gcagatcgcc tgccgcgaaa tcccacggac gcagaccgat ttcaaagaaa ccgtcaacac
    15421 gacccgcagc gacgtaagcc agatccagcg ccgcagaacc ggtacgacgg aagtctgcac
    15481 attcgttgaa cagcttgccg acgatgttga tgtaggtagt ggcgtactgt tttgctttga
    15541 acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg agcggtgcta ccgcgcagac
    15601 ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt gaacagttcg ttacgcatag
    15661 gatcgtatac cacagcaact tcggtgcggc ctttgatacg aacggcgata gataccgcga
    15721 agtgcggcag acgtttgata aagttggtag taccatccag tggatcgata acccattgaa
    15781 catcctgatc agtaccttca agttcaccgc tttcttcggt gatgatggtg tgctgtgggt
    15841 aagatttacg aatcgtgtcg ataatcaccg cttcggcagc tttatctacg ttggtcacga
    15901 aatcgttact gcctttctgg ctcgcttcta cagcgtccgg ggtttcatag tttttggcaa
    15961 ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt cagcatcgga tgcatcagtc
    16021 tctctcactg gatgttaaag aacgggaaaa cggcgcagag tatagcagcg agatcggcat
    16081 ctgtcttcgt tttatgataa gatgcgtgaa taatctttag atgaagaaag acaatgctgc
    16141 aaaatattcg aattgtgctg gtggagacgt cacacaccgg caatatgggt tctgttgccc
    16201 gtgccatgaa aacaatggga ttaaccaatc tgtggctggt taatccactg gtgaaacccg
    16261 actcccaggc gattgccctg gctgcagggg ccagcgatgt gattggtaat gctcacatcg
    16321 tcgatacgct cgacgaagcg ttagccggtt gtagcctggt ggtgggcacc agcgcacgct
    16381 cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg cggtctgaaa agcgtcgctg
    16441 aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga gcgcgtcggc ttgaccaatg
    16501 aagagttgca gaaatgccat tatcatgtcg cgattgcggc aaacccggaa tacagttcgc
    16561 tgaacctggc aatggcggtt caggttattg cctatgaagt gcgtatggcc tggctggcaa
    16621 ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc gtatccgctg gtcgatgatc
    16681 tggagcgttt ttatggtcat ctggaacaaa cgctgctggc aaccggtttt atccgtgaaa
    16741 accatccggg gcaggtgatg aataaattgc gccgtctgtt tacccgtgcg cgcccggaaa
    16801 gccaggaatt gaatatcctg cgcgggattc tggcttctat tgagcagcag aataaaggta
    16861 acaaggccga ataacagtgg ttgcctgatg cgacgcttaa cgcgtcttat caggcctaca
    16921 atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca tccgacaaca ggtacaaacg
    16981 ccacgataaa aaaatggcac tgaaggttaa atacccgact aaatcagtca agtaaatagt
    17041 tgaccaattt actcgggaat gtcagacttg accctgctat gcaatacccc cacttttaca
    17101 ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa gacatgagac tgacatctaa
    17161 agggcgctat gccgtgaccg caatgcttga cgttgcgctc aactctgaag cgggcccggt
    17221 accgttggct gatatttccg aacgtcaggg aatttccctt tcttatctgg aacaactgtt
    17281 ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt ggaccaggcg gtggttatct
    17341 gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg attagcgccg ttgacgaatc
    17401 tgtagatgcc acccgttgtc agggtaaagg cggctgccag ggcggcgata aatgcctgac
    17461 ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt tttctcaaca acattacttt
    17521 aggcgaactg gttaataacc aggaagtgct ggatgtgtct ggtcgtcagc atactcacga
    17581 cgcgccacgc acccgcacac aagacgcgat cgacgttaag ttacgcgctt aataaaaaga
    17641 attcagaatc aggccggagt gctaaatact ccgtaaacac ggtcgtacat ccagccggta
    17701 gcctgattcc ttgcattgag tgatgtacgg agtttataga gcaatgaaat taccgattta
    17761 tctcgactac tccgcaacca cgccggtgga cccgcgtgtt gccgagaaaa tgatgcagtt
    17821 catgacgatg gacggaacct ttggtaaccc ggcctctcgt tctcaccgtt tcggctggca
    17881 ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc gatctggtcg gcgctgatcc
    17941 gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac aacctggcga tcaaaggtgc
    18001 agccaacttt tatcagaaaa aaggcaagca catcatcacc agcaaaaccg aacacaaagc
    18061 ggtactggat acttgccgtc agctggagcg cgaaggtttc gaagtcacct acctggcacc
    18121 gcagcgtaac ggtattatcg acctgaaaga actcgaagcg gcgatgcgtg acgacaccat
    18181 ccttgtttcc atcatgcacg taaataacga aatcggcgtg gtgcaggata tcgcggctat
    18241 cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt gatgcaaccc agagcgtggg
    18301 caaactgcca atcgacctga gccagttgaa agttgacctg atgtctttct ccggtcacaa
    18361 aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt cgtaaaccgc gtgtacgcat
    18421 cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg cgttccggca ctctgcctgt
    18481 tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca aaagaagaga tggcgaccga
    18541 gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac ggcatcaaag atatcgaaga
    18601 agtttacctg aacggtgacc tggaacacgg tgcgccgaac attctcaacg tcagcttcaa
    18661 ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac ctcgcagttt cttcaggttc
    18721 cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg cgcgcgctgg ggctgaacga
    18781 cgagctggca catagctcta tccgtttctc tttaggtcgt tttactactg aagaagagat
    18841 cgactacacc atcgagttag ttcgtaaatc catcggtcgt ctgcgtgacc tttctccgct
    18901 gtgggaaatg tacaagcagg gcgtggatct gaacagcatc gaatgggctc atcattaatc
    18961 ggtatcggaa tcaggagaat ttataatggc ttacagcgaa aaagttatcg accattacga
    19021 gaatccgcgt aacgtgggtt cctttgataa caacgacgag aacgtcggca gcggcatggt
    19081 gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt aaagtcaacg atgaaggtat
    19141 cattgaagac gcgcgtttta aaacttacgg ctgcggttcc gctatcgctt ccagctccct
    19201 ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg caggcgatca aaaacaccga
    19261 tattgctgaa gaacttgaac tgccgccggt gaaaattcac tgttctattc tggcagaaga
    19321 cgcgatcaaa gccgccattg cggactataa aagcaaacgt gaagcaaaat aagagttgag
    19381 gtttggttat gtcgattaca ctgagcgaca gtgcagcagc gcgagtaaat acctttctgg
    19441 ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag aacctccgga tgttcaggta
    19501 tggcttatgt actggaattt gttgacgaac cgacgccgga agacatcgtg tttgaagaca
    19561 aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt tctggacggt acgcagctgg
    19621 acttcgtaaa agaaggcctg aatgaagggt ttaaattcac caacccgaac gttaaagatg
    19681 agtgtggttg cggcgaaagc ttccacgttt gatgcgcata ccgataaccc caccgtggtc
    19741 gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc gcggcggatc gcagccctga
    19801 gaatgttatg gattacttca ccctctttgg cttgcctgcc cgttatcaac tcgataccca
    19861 ggcactgagc ctgcgttttc aggatctaca acgtcagtat catcctgata aattcgccag
    19921 cggaagcctg gcggaacaac tcgccgccgt acagcaatct gcaaccatta accaggcctg
    19981 gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg ctttctttac acggttttga
    20041 tctcgccagc gagcagcata ctgtgcgcga caccgcgttc ctgatggaac agttggagct
    20101 gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa gcgcggctgg aaagctttat
    20161 caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg atggttgaac agttagacaa
    20221 cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg cgttttctcg ataaactgcg
    20281 gagcagtgcc gaacaactcg aagaaaaact gctcgatttt taatttctgg aagctaaaca
    20341 tggccttatt acaaattagt gaacctggtt tgagtgctgc gccgcatcag cgtcgtctgg
    20401 cggctggtat tgacctgggc acaaccaact cgctggtggc gacagtgcgc agcggtcagg
    20461 ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc atctgttgtt cactatcaac
    20521 agcaagggca ttcggtgggt tatgacgcgc gtactaacgc agcgcttgac accgccaaca
    20581 ccattagttc tgttaaacgc ctgatggggc gctcgctggc tgatatccag caacgctatc
    20641 cgcatctgcc ttatcaattc caggccagcg aaaacggcct gccgatgatt gaaacggcgg
    20701 cggggctact gaacccggtg cgcgtttctg cggacatcct caaagcactg gcggcgcggg
    20761 caactgaagc cctggcaggc gagctggatg gtgtagttat caccgttccg gcgtactttg
    20821 acgatgccca gcgtcagggc accaaagacg cggcgcgtct ggcggggttg catgttctgc
    20881 gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg gctggattcc ggtcaggaag
    20941 gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga tatttccatt ctgcgcttaa
    21001 gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc cgcgctcggc ggcgatgatt
    21061 tcgaccatct gctggcggat tacattcgcg agcaggcgga cattcctgat cgtagcgata
    21121 accgcgttca gcgtgaactg ctggatgccg ccattgcagc caaaatcgcg ctgagcgatg
    21181 cggactccgt gaccgttaac gttgcgggct ggcagggcga aatcagccgt gaacaattca
    21241 atgaactgat cgcgccactg gtaaaacgaa ccttactggc ttgtcgtcgc gcgctgaaag
    21301 acgcgggtgt agaagctgac gaagtgctgg aagtggtgat ggtgggcggt tctactcgcg
    21361 tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg tccaccgctg acttccatcg
    21421 acccggataa agtcgtcgct attggcgcgg cgattcaggc ggatattctg gtgggtaaca
    21481 agccagacag cgaaatgctg ctgcttgatg tgatcccact gtcgctgggc ctcgaaacga
    21541 tgggcggcct ggtggagaaa gtgattccgc gtaataccac tattccggtg gcccgcgctc
    21601 aggatttcac cacctttaaa gatggtcaga cggcgatgtc tatccatgta atgcagggtg
    21661 agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt tgcgctgcgt ggtattccgg
    21721 cgctaccggc tggcggtgcg catattcgcg tgacgttcca ggtcgatgcc gacggtcttt
    21781 tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc gtctattcag gtcaaaccgt
    21841 cttacggtct gaccgatagc gaaatcgctt cgatgatcaa agactcaatg agctatgccg
    21901 agcaggacgt aaaagcccgt atgctggcag aacaaaaagt agaagcggcg cgtgtgctgg
    21961 aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt aagcgccgca gagcgtcagg
    22021 tcattgacaa tgctgccgct cacctgagtg aagtggcgca gggcgatgat gttgacgcca
    22081 tcgaacaagc gattaaaaac gtagacaaac aaacccagga tttcgccgct cgccgcatgg
    22141 accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga cgaggtttaa tatgccaaag
    22201 attgttattt tgcctcatca ggacctctgc cctgatggcg ctgttctgga agctaatagt
    22261 ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg agattgaaca cgcctgtgaa
    22321 aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg aaggttttga ctcactgccg
    22381 gaaagctcag agcaggaaga cgacatgctg gacaaagcct ggggactgga gccggaaagc
    22441 cgtttaagct gccaggcgcg cgtcaccgac gaagatttag tggtcgaaat cccgcgttac
    22501 actatcaacc atgcgcgtga gcattaacag aggttagtat gggacttaag tggaccgata
    22561 gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga tcttgatccg aaaacggttc
    22621 gattcaccga tatgcatcag tggatttgcg atctggaaga tttcgacgac gacccgcagg
    22681 catccaacga gaaaatcctc gaagcgattt tgttagtctg gctggacgaa gccgagtgaa
    22741 tgaacgggct gcctgggggt agcccgtttt gctgttaaac gaataaggat aactaaaatg
    22801 acagaagcga tgaagattac cctctctacc caacctgccg acgcgcgctg gggagaaaaa
    22861 gcaacttaca gcattaataa tgacggcatt accctgcatt tgaacggggc agacgatctg
    22921 gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg gcatcaagca tgttcagtta
    22981 agcggtgaag gctgggatgc ggatcgctgc tgggcattct ggcaaggtta taaaggcccg
    23041 aaaggcacgc gtaaagtgga gtggccggat ctggacgatg cccagcgcca ggaactggat
    23101 aaccgcctga tgatcatcga ctgggtgcgt gacaccatca acgcaccggc agaagaattg
    23161 ggaccatcgc aactggcaca gcgtgctgtt gatctgatca gcaacgtcgc gggcgatcgt
    23221 gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc aaggttatat ggggctgcac
    23281 actgtcgggc gcggttcaga acgttctccg gtattgctgg cgctggatta caacccaact
    23341 ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta aaggtatcac ttttgactcc
    23401 ggcggctaca gcatcaaaca gactgcgttt atggactcga tgaagtcgga catgggcggc
    23461 gcggcaacgg ttaccggggc gctggcattt gccattacgc gcggactgaa caagcgcgtg
    23521 aagctgttcc tctgctgtgc ggataacctg attagcggca atgcgttcaa gctgggcgat
    23581 atcatcactt atcgcaacgg taaaaaagtt gaagtgatga acactgatgc cgaagggcgt
    23641 ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga aaccggaaat gatcattgat
    23701 gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta atgattatca cgcgctgttc
    23761 agttttgacg atgcgcttgc cggtcgttta ctggcgagtg cctcacaaga gaacgaacca
    23821 ttctggcgtc tgccgctggc ggaattccac cgcagccagc tgccgtctaa ctttgccgaa
    23881 ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga gcacggcggc gggcttcctg
    23941 tcgcactttg ttgagaacta tcagcaaggc tggctgcata tcgactgctc ggcgacttac
    24001 cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg gacttggtgt gcgcacgata
    24061 gctaatctgt taacggcgta aactaatatg ccggatgcgg cgtaaacgcc ttatccggcc
    24121 tacaaattct gcaaattcaa caaattgcga atcccttgta ggcctgataa gcgtagcgca
    24181 tcaggcattt gcgccccgaa cgttgaatga attgtggttc ttatgtccga aacaaaaaac
    24241 gaacttgaag acctgctgga aaaagcagca accgaaccgg cgcaccgccc ggcctttttc
    24301 cgtactctac tggaatccac cgtctgggtg cctggtacgg cggcgcaggg cgaggctgtg
    24361 gttgaagatt gtgcgcttga tttacagcac tgggaaaaag aagacggcac cagcgtcatt
    24421 ccttttttca cctcgttaga agcgcttcaa caggcggttg aagacgaaca ggcatttgtc
    24481 gtaatgcccg ttcgcacatt gtttgagatg acccttggcg aaacgctctt ccttaatgcc
    24541 aaactgccga ccggtaaaga atttatgccg cgtgaaatca gtttgttgat tggtgaagag
    24601 ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg aatcgctgat attatcggaa
    24661 gtcgcagagc cgccagcaca aatgattgat tcactcacca ccttatttaa aaccattaag
    24721 ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg aagaggcaca gcctaattta
    24781 cttattggca ttgaagccga tggtgatatc gaagaaatta ttcaggcgac gggaagtgta
    24841 gcgaccgata cattacctgg cgatgaacca atcgatattt gtcaggtgaa aaaaggggaa
    24901 aaaggaatta gccactttat taccgaacat attgcgccat tctatgaacg tcgctggggt
    24961 ggttttttgc gtgactttaa acaaaaccgg ataatctaaa atatgccgcc ctaaagggcg
    25021 gcattcgggc ttacttgctg ctctgaaggt tgttaaccac cagtaaattg tcgccggaga
    25081 gactccggtt attatttact tgggaaaaca gcgcaccaga aacaataagt acgatacaga
    25141 agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg catcacgaca gcaccactgg
    25201 cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc taacggtcgc ggaacatcct
    25261 ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg ccgcagtgga taatacacta
    25321 acagaacaat aataactctc ttttgcttga caaaaagaga gttactggcg agtattgtct
    25381 ccgctgagac gcgcttgcat tactacggta atacaacctg ctggaaaggc cgcgaaccag
    25441 accagcaata aaaaaacgcc aaattcggcg gttttttatt gcctgtaaaa tacttatttc
    25501 actggctcaa ccggtaaatc tgcccgcgcg ccccattcac tccatgcgcc gtcgtacagt
    25561 ttcacgtttg gcacatccag cgtcgcgagt gctaacaaaa ccacggctgc cgttacacca
    25621 gagccgcagc tgacgataat cggtttgtca tagctgacgc cgcgaccaaa aaatatcgca
    25681 tccagttcat cggtcgtttt tagttcgcct tcgcgcacca gttccgtcca cggaacgttc
    25741 aatgctccgg gaatatgtcc gcgacgtaaa cctgggcgag gttcatcaac ttctgcgtta
    25801 aaacgtgcag ccgggcgggc atcaataatt tgcgccgtat tttcatggct tgccaataac
    25861 acatcggtta ctttcaccac tgcttcagga ttaaacgcgg cgttaaactc tccttccggc
    25921 agctctactg caccttcttc taatagcagg tcatcgcgct gccagcctgc aag
//
LOCUS       DAJTYI010000045        25721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYI010000045 DAJTYI010000000
VERSION     DAJTYI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            178..471
                     /locus_tag="OO564_001844"
     CDS             178..471
                     /locus_tag="OO564_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6652991.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            543..1202
                     /locus_tag="OO564_001845"
     CDS             543..1202
                     /locus_tag="OO564_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6652992.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1294..1740
                     /locus_tag="OO564_001846"
     CDS             1294..1740
                     /locus_tag="OO564_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6652993.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1947..2847)
                     /gene="hlyE"
                     /locus_tag="OO564_001847"
                     /pseudo
     CDS             complement(1947..2847)
                     /gene="hlyE"
                     /locus_tag="OO564_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3220..3639
                     /locus_tag="OO564_001848"
     CDS             3220..3639
                     /locus_tag="OO564_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6652994.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3639..4905
                     /gene="umuC"
                     /locus_tag="OO564_001849"
                     /pseudo
     CDS             3639..4905
                     /gene="umuC"
                     /locus_tag="OO564_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(4951..5481)
                     /gene="dsbB"
                     /locus_tag="OO564_001850"
     CDS             complement(4951..5481)
                     /gene="dsbB"
                     /locus_tag="OO564_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6652995.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5627..7168)
                     /gene="nhaB"
                     /locus_tag="OO564_001851"
     CDS             complement(5627..7168)
                     /gene="nhaB"
                     /locus_tag="OO564_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6652996.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7389..8108
                     /gene="fadR"
                     /locus_tag="OO564_001852"
     CDS             7389..8108
                     /gene="fadR"
                     /locus_tag="OO564_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6652997.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8160..9692)
                     /locus_tag="OO564_001853"
     CDS             complement(8160..9692)
                     /locus_tag="OO564_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6652998.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10022..11320
                     /gene="dadA"
                     /locus_tag="OO564_001854"
     CDS             10022..11320
                     /gene="dadA"
                     /locus_tag="OO564_001854"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6652999.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11330..12400
                     /gene="dadX"
                     /locus_tag="OO564_001855"
     CDS             11330..12400
                     /gene="dadX"
                     /locus_tag="OO564_001855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6653000.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12568..13994)
                     /locus_tag="OO564_001856"
                     /pseudo
     CDS             complement(12568..13994)
                     /locus_tag="OO564_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14604..16340)
                     /locus_tag="OO564_001857"
     CDS             complement(14604..16340)
                     /locus_tag="OO564_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6653001.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16435..17349)
                     /gene="ldcA"
                     /locus_tag="OO564_001858"
     CDS             complement(16435..17349)
                     /gene="ldcA"
                     /locus_tag="OO564_001858"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6653002.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17449..18060
                     /gene="emtA"
                     /locus_tag="OO564_001859"
     CDS             17449..18060
                     /gene="emtA"
                     /locus_tag="OO564_001859"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6653003.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18062..18796)
                     /locus_tag="OO564_001860"
     CDS             complement(18062..18796)
                     /locus_tag="OO564_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6653004.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            18997..19251
                     /locus_tag="OO564_001861"
     CDS             18997..19251
                     /locus_tag="OO564_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6653005.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19301..20761)
                     /locus_tag="OO564_001862"
     CDS             complement(19301..20761)
                     /locus_tag="OO564_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6653006.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20698..21255)
                     /locus_tag="OO564_001863"
     CDS             complement(20698..21255)
                     /locus_tag="OO564_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6653007.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21281..22135)
                     /locus_tag="OO564_001864"
     CDS             complement(21281..22135)
                     /locus_tag="OO564_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6653008.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22135..22944)
                     /locus_tag="OO564_001865"
     CDS             complement(22135..22944)
                     /locus_tag="OO564_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6653009.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(22954..23712)
                     /locus_tag="OO564_001866"
     CDS             complement(22954..23712)
                     /locus_tag="OO564_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6653010.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23709..24689)
                     /locus_tag="OO564_001867"
     CDS             complement(23709..24689)
                     /locus_tag="OO564_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6653011.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24689..25711)
                     /locus_tag="OO564_001868"
     CDS             complement(24689..25711)
                     /locus_tag="OO564_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6653012.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6403 a         6336 c         6691 g         6291 t
ORIGIN      
        1 attccagtgg cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat
       61 tacttataaa ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg
      121 cgcgggtttc attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg
      181 ttttgtgtga tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa
      241 gacgattttt cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg
      301 atgattctgc cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa
      361 ctggcattaa ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg
      421 aagcaacatc tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc
      481 ggcggtggca ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg
      541 ttatgtatca acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag
      601 tctgtgttgg cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag
      661 agccagtgct gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga
      721 tcccatccga tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga
      781 cgctgcgtca ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc
      841 ttgatctgac attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa
      901 aggctaaagc gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta
      961 ccggcgatcc acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag
     1021 gtacgactgc ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt
     1081 ttaccctcaa ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc
     1141 aaagcggtga tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt
     1201 aatacttttt gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat
     1261 aaggtgcgtt ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt
     1321 aaaaccctgg acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag
     1381 tgttgcctgc ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc
     1441 tgtcgccagc tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt
     1501 gaacccgact gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg
     1561 acctgcgctt atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt
     1621 actggttcga aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa
     1681 gaatcagaag tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga
     1741 taaatttaat agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg
     1801 tagccctgtt tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac
     1861 aggtaactct caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt
     1921 gagtacatgg agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta
     1981 ccgtgtcttt tctgatactc attacaggtg ttaatcattt ttttggccgc ttcttttagc
     2041 aaagaaagca ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt
     2101 atctccccga tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta
     2161 ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg
     2221 ttcttcaatt ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg
     2281 attaaaccaa atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg
     2341 attttatcta cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg
     2401 ctatctaacg ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg
     2461 gatttttgcg cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga
     2521 tgctttcttc tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc
     2581 aacaccacac cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat
     2641 aagtaaggtt ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa
     2701 gcgacttaac tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata
     2761 tttattataa agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac
     2821 cgttttatct gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa
     2881 tgaaactcct gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg
     2941 gcgggatgcg tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg
     3001 tcattatggc gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg
     3061 gatgagcgtc gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag
     3121 tctatgcagc gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac
     3181 tactgtatat aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg
     3241 gatctccgcg aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct
     3301 tcaccggcag cagattacgt tgaacagcgc atcgatctga atcaacggtt gatccagcat
     3361 cccagcgcga cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt
     3421 gacggtgatt tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc
     3481 gctgctgttg acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt
     3541 attcccatga acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc
     3601 tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa
     3661 acgcgtttta tgccagctgt gagacggtgt ttcgccctga tttatggggt accggtggtt
     3721 gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc
     3781 gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt
     3841 tgctttagca gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg
     3901 gaagaactat cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca
     3961 ggtgtgcgta attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta
     4021 caacgtaccc atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt
     4081 gccaatcatg cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat
     4141 ctggaacgcc agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga
     4201 cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat
     4261 acagatatcc ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa
     4321 ctgcgcggcg aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc
     4381 tgttcccgct cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt
     4441 agttacgctg cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc
     4501 tccacattta ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcacg
     4561 tcggtaaaac tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga
     4621 tctctggatg ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg
     4681 gatttcttca gtcagggagt cgcgcagctc aatttattcg atgacaatgc accgcgcccc
     4741 gggagtgagc aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca
     4801 ctctattttg ccgggcaggg gatccaacag caatggcaga tgaagcgagc tatgctttca
     4861 ccacgttata caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa
     4921 aaaagcgttc ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc
     4981 tttaaacggc tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc
     5041 gagcagccac tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc
     5101 agaggcgaca aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac
     5161 cataaaatca caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt
     5221 taactgcaca ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg
     5281 agttttcggg gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg
     5341 ttcataaata cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt
     5401 cagttccagt gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca
     5461 ttggttcaaa aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa
     5521 accaattcat tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat
     5581 cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta
     5641 tccagcccat ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca
     5701 agccgacgag tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag
     5761 agaggcgaat caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt
     5821 tcggcgtagc gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt
     5881 attgcttcaa cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg
     5941 tccccacgaa gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg
     6001 acagctgagc atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt
     6061 ggtcgataat caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag
     6121 attcggtgaa ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca
     6181 gaataatgac tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca
     6241 ccagccagac gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct
     6301 ggtggcggct ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt
     6361 caccgtagcc aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca
     6421 gaaccggaac ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg
     6481 ctttagcgat gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg
     6541 tgccgacacc ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca
     6601 cgttgtaatg cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag
     6661 aggctacgcg atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga
     6721 cggttaaggc atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca
     6781 gcagcatttt ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt
     6841 tcataaaata gatacccgcc accataaaca tcaataacag caagacttca agatttgccg
     6901 ccaccccttc acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca
     6961 acagaccacc ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt
     7021 ccgctaccag caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga
     7081 ttaagaaaat gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc
     7141 atagcgcgcg gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg
     7201 atgtttttgt gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata
     7261 gtcaagggga aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc
     7321 tatcagcgta gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa
     7381 atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg
     7441 aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt
     7501 cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag
     7561 atggctggtt gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt
     7621 ccggtttaaa tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta
     7681 ttgataattt gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc
     7741 gtcagcatcc cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg
     7801 ccgatgcctt tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca
     7861 acccgattta cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc
     7921 actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg
     7981 cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg
     8041 agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg
     8101 ggcgataatc ccttccgttt aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt
     8161 tacagatttc ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt
     8221 tgctgttcga gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg
     8281 cgcccccgat ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg
     8341 ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc
     8401 gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc
     8461 agataattat gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc
     8521 ggtgacagaa actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc
     8581 gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc
     8641 gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg
     8701 ccgctgtacc acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa
     8761 aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta
     8821 tagtgccaga aggtcgcaca gccttcgttc ataacctgag tctgtttttg cggataaaaa
     8881 tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc
     8941 gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct
     9001 tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga
     9061 ctttgtagat actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt
     9121 tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc
     9181 cgttctactt catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa
     9241 atcagataat cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta
     9301 aagaaagagt tatgcccata gcaggtgtgt gccatcacca gcgcctgcat agtaatggtg
     9361 ttctcttcca tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc
     9421 ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag
     9481 tgcggatagt taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt
     9541 atcacttcaa tctggtgcgg gtaagtatcc agccggtaga gtttcgccac ccggtctatc
     9601 tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg
     9661 gtgtccttat taatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct
     9721 ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa
     9781 tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt
     9841 tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt
     9901 cagggagatg tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg
     9961 agttatcaag atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc
    10021 tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa
    10081 tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag
    10141 tgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt
    10201 gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga
    10261 cggtacgcag tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca
    10321 ctacatggaa aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa
    10381 agcattgcgc gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact
    10441 gttccgtacc gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc
    10501 cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc
    10561 agaagtagcg cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg
    10621 ccagctgttt acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt
    10681 taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg
    10741 cgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat
    10801 gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc
    10861 aattgcgcag gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc
    10921 cattacccgt ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa
    10981 caccgagctg ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc
    11041 acgcggcggt catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga
    11101 cggtacgccg gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg
    11161 cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg
    11221 tcgcacgcca gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac
    11281 gccatcacgt ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc
    11341 gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc
    11401 cgcgccgcac gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga
    11461 gcgtatctgg agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc
    11521 aataacgtta cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca
    11581 tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg
    11641 gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt
    11701 gaacagtggg atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca
    11761 gttgcgggca atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga
    11821 acatcctgat ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga
    11881 gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga
    11941 ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat
    12001 cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac
    12061 gctaaaagcg ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg
    12121 aatcggcatt gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtac
    12181 tcctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct
    12241 ggcggttgat ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg
    12301 caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat
    12361 gtgcgcgctg gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg
    12421 aggcagcgtc ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt
    12481 taatgtgaca gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc
    12541 ggaacctgaa ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc
    12601 ggagtcgact tagataaatc acgcttcacc cttgatgcag attcgggttc atgttcagat
    12661 tgttgattaa caggctgcga gtcctgttgt ctttccagac tcaacaggcc caactgttga
    12721 tccaggagct ccaggtcctc ctgtaccttc acgcgctcca ggagttccgg gtcatccagg
    12781 aactccagat ccataaggtc ctccagctcc tcgagtcctt ccaggtcaac ctagccttcc
    12841 ttgtgcaaaa agtccaaaag gtccttcagg ccctgctgtt ttttcaggcc ctcgacggct
    12901 ttgagcagcg tcggtaaatt gtctttgtcc cacgggttac cattacagtc aaacaccttc
    12961 aggctctggg gctgcggcac ctgtaccgtt ttaaacttgt tttctccaca gtagtaaatt
    13021 attagtttgg gcggcagccg tggtgaagag atcaaatcgt tactagaaca gtcaaatact
    13081 ttcaggcgag tcaagttctc cagcatctca tcaggtattg agttaatatt gttgccggca
    13141 caggatagtt tatacaggta ctggggcatc gacggcggta aaaattgcaa gaagttttca
    13201 gaacagttaa tcactctcag gttagtcgac agctcaggca gccagataat acagttttca
    13261 tagcacttca gtaccatcag ttttgagggc tgcaccttcg gtaacaagat caagacgtta
    13321 tctgtacagt caagcaccag cagccacttt ggcatcacct tcggtaacgc ggtcaacacg
    13381 tttttactgc agtaaaattc aatcagatgt ggaggcaaat tctcagggag tgaagtcaat
    13441 cccaggttgc taagattgag catactccgt cttgtaatga gacattcctt cattctgcgc
    13501 aatgcttctg cccggttttc aatccggctt gctggcaggt tttcttccag gatattttcc
    13561 ttgatttctt ccagactttc tttcaggtct tcttggggaa tttctgactg tttgttttgc
    13621 tggttttctt ccaggatttc gtacaggttt ttttgcaggt tttcttgcaa accatcctgc
    13681 tgtatagccc attcgcacca tttggcgacc atcatgaact cgttatgttg ctgattcagg
    13741 cgagtcccca acgtgatgat ggtattgttg tccacctgta tgttcacttt gcccgcgata
    13801 ctgaggtcaa tgatctgcca gggggcatca ccccagctga atttttgcgg gctgagatac
    13861 ttcatgttct gcaaaatttg attcttgagt tccaccgaca ggaagagccg ccctggcggg
    13921 cgtcgtacgt ctgggttaac ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct
    13981 ttaaagctag ccataatgct acgtttcctt gaaataaata tcctctggca gcgccggaga
    14041 cgtgaatgtg gtcaccgcaa aggagccaca ggacgtcatt tgcgctgata tcactactca
    14101 tcgttgactt tttccctggt cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg
    14161 cctaggtagc tctgacgtat taaagaaaat acgagagtga acgacccgga aataatcgtt
    14221 attaagcaaa ggaataacta taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa
    14281 acgcctattg tgtgggctat gtcagtgcgg caggttgcgt ggggaatatg ttcaggcgat
    14341 gtcaggcagg ggcccctgat ggttgaccgg taatgcgggg ctggttttta ttacctgtta
    14401 cagtcggaaa aagcccttac gctgactttc ttgagccggt gttttgagtt cattagccag
    14461 agcctgcaat ggtttgtcat ccataattta accttcttct gatgttggga tgagcatatc
    14521 aaaatcagga aatgacaagt ttattacagg ctcaggctgc gttcagtaaa gcatcgcatc
    14581 cggcgataga tgctgcatta aatttaagat tcagcttcct cttcagccac ccgaacgcca
    14641 atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct
    14701 acctggtcac cgacaaccgg tgccgcgcct agcaactgct ggacaatttc acccagcgtt
    14761 tgctgtttat cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagca
    14821 tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggt
    14881 ggcgactggc tgaacagttt accgagcgcc gggagatcgc gttcccgacc aattacacac
    14941 aatacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga
    15001 aacagggctg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc cgcacctaca
    15061 caccatttat cagcactcaa ttgataaaca aactgctccc acggattttc cggatgaata
    15121 tccaggccaa cgcgtgacac cggacgtccg acgggcggaa ccaccacttt ggcttttttc
    15181 gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg
    15241 acattaaaga acagtcgcgc attctccagc cccgccatca tcgggaacac ggccaggatg
    15301 attggcaccg cgccgcgtaa tcccacccag ctgataaaca cgcgctcacg cagattgaaa
    15361 ccgcggaaag ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat
    15421 gccgacaaaa tgagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc
    15481 cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg
    15541 atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc
    15601 agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat
    15661 gccagcaatg gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa
    15721 taaccaccgc caagaccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg
    15781 aacatccaac tgacactgct ttcatgctgc tggatcattg caatcagggt aatcgtcaga
    15841 aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg
    15901 ttaagcccct taccacccag cagagaaaag accgctgcag catcggtgga gccgacgata
    15961 gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg
    16021 gttaaaccag aggtgataag tacgcccagc gtcgccagcg acagcgccgg tcctaacgcc
    16081 acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagcgcc
    16141 aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg
    16201 actcccgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa
    16261 gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta
    16321 attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc
    16381 ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat
    16441 tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat
    16501 ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt
    16561 aatgagtgga atcgacaggc gggaacgcaa aaacgcgtac actgactcca ggttgtaacc
    16621 tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg
    16681 tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc
    16741 gttaatatct tccagcacca gaataccgtt ctcaattttt ggcatccacg gtgtaccaat
    16801 cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg
    16861 gccttcccct tgccattcaa tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg
    16921 ctcggtaaag gcgttcagtt catccgcacc aaagtttgct accagcatgg ggccgctaaa
    16981 ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc
    17041 gcaaatgagt aacgggtcat gttgttggcg agccaccagt gcctgccagt caatatctgc
    17101 cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggggtagt
    17161 cagtctggca agggaattga gatcttccag acgctccgtt tccgtacccg caaaacgctc
    17221 acaacgacgg gcaataacct cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg
    17281 gataccacgc agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa
    17341 cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt
    17401 gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga
    17461 tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac
    17521 ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag
    17581 aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa
    17641 tcgggtggta atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa
    17701 gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc
    17761 agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg
    17821 gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc gctggtggtg
    17881 tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg
    17941 atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgaaatcat
    18001 cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa
    18061 atcagtcgcg cactttgtcc gctttttccc gggcttctcg ctcgagagag aaaataatcc
    18121 gctgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag
    18181 tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctggtggag attaactggg
    18241 cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatttgagcg aagcgcatac
    18301 cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca
    18361 aatcatacaa gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag
    18421 gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg
    18481 gaggcggtac ggtaataaat gccggaagct gcaagtattc actctgctgt agttgttcaa
    18541 cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta
    18601 gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta
    18661 ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg
    18721 ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct
    18781 catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc
    18841 atagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag
    18901 ccgacaaacg cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat
    18961 ttcttgcgac cgattcatcg aaacgagggc ctgaacatgg gaattattgc ctggattatt
    19021 tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga
    19081 tttttcctga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc
    19141 atgtttggca ttggcggctc catcagcggt tttaatttgc acagcttcct agtggcggtg
    19201 gtgggcgcta tcctggtact gggcgtattc cgcctcctgc gaagagaata agattttcat
    19261 aaggcggata gcgatacaga tgccgctatc cgctttcaca tcagaacgta tactcgacac
    19321 ctgcccagaa attgcgacct tcttcaataa acccttcact gtaggcatat tgggtatcaa
    19381 acaggttatt gaccgacgca ttgacgctga agccatgacc taaggtgtaa tcggctcgaa
    19441 tgtgggtcac cgcaaaaccg gcggcttttt gtgaaccgtc actgttgctg tagctggagg
    19501 aacgcgcctc ttccgacagc gttacgctta acggctccca tggtttgagt gtcatccatg
    19561 cggtcattgt ctgcgttgga agatcggtta tcttgccgat gtctttgcgt ttggcgtcag
    19621 cgtggatcag agcgtagctc aatcctacat ccagtatatt gctgattttc cccttaatac
    19681 cggcatccag accgctgtaa tccaccgtgc cgctgttctg attttgaatg gtatcggcat
    19741 tgatattgtg cgagaggatg gcatcactca cccggttata gtaaacgctg acctcaaatc
    19801 cccagtcgcg cgtgaaggca ccattccagg ttaaatccac cccgcgagcg cgttccggct
    19861 tgagctgcgg gttaacaatc gctatctggt tatacgcagg tttggatgtg gtatagcgtt
    19921 ctttcagcgt cggaaagcgt gtgcggtcat agtacgaaag cgccagagtg tcttcattgg
    19981 caaagtggta tttccccatc acctgccagt taaaagctga ctgattgttg tcgtcataat
    20041 gggtgatgct gccatctttc tcgtgttttt tcgcttctac gctatcgcgc cagtcatagc
    20101 tgattcccgc cacgacatcg acattatcag cagcagccca ttgatattca ctggcgagcg
    20161 accaggtacg atcttcatag cgatcgtaag cggcgtgcgg cgcacctttt tcccggtgta
    20221 cgtcatcttt ccagttaacg gcaaacgaca gcagatcgtt ttcacgcaca tcggctgcca
    20281 gttgtaatcc ggcaccgtcg ctgtaatcgg aataatggct gtagctgcct tttttatttt
    20341 tcaaatcagc cagcgagttg tacatcatca gcgtattttc aaaggtgtcg cgatacagcc
    20401 gacttttcag ggtaaaacgg tcgtttagtt gggtcgctcc ctgataataa aaactttctt
    20461 tgtcatactc tggccactgc cagtagcgtg atttttgacc acaatttccg ctgtatggcg
    20521 ggttatcttt ttcaccatcc tgcttaatgt aagtcagcgt gtattcatcg ttttcgcgcg
    20581 gtgtaaaacc gagcttcaca atgccgcgtt tatcatcagc cgatgaatta atcatcttgc
    20641 cgtgtttgcc tgcaatatca ttatttacac catgcggcag gccgagaaaa tcctgcttta
    20701 gctggctacc gctgacttgc aaatacccaa gatcgctgct ggcggcaaat gaagcatgca
    20761 tatcgtaggc attgtcctga ctacggctcc atccctggcg atatcccaga cttgcttctt
    20821 cagggtggtg atattaatgg ctccgcccat ctgattaggc ccatgaagca gcgacgaata
    20881 ccctttggaa acttcgactg cccccagatt gttggtcaga acccgcgcca gatcgaggtt
    20941 gccgtcatag ggaacataaa tgggcacacc gtcgaaatag accggcacct gccgactatc
    21001 aaagccacga acttttacct gttcttcgtt gcggctgcct gacttttgca gcaccacgcc
    21061 ggggacgaca cttaatgcct gagcgacatt ctgtttatcc aaggctttca tggtgggttg
    21121 atcgagaacg gtggtcgtcg tcgatgatac cggactggac catacggtca gcgtgtcgct
    21181 ctcctgagcc gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg
    21241 ttttttgagt ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt
    21301 gcagactgac tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc
    21361 gaatgccgca gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca
    21421 gcgtgcagtg aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac
    21481 taaatttgtc gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg
    21541 gcgtgtcggc ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg
    21601 cgcctgacca gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg
    21661 tttccgcaca tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca
    21721 gtaagcgggt gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt
    21781 cacgaagcaa tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca
    21841 cattctggac tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta
    21901 gccgttgacc cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca
    21961 gcgtagttaa catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa
    22021 aaaacgctat atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc
    22081 cctggatatc ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag
    22141 tgcctcctgc ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag
    22201 ggcacgagca tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag
    22261 cgcgccaaga ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg
    22321 gcaattttgt ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt
    22381 gagcgcaaag atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc
    22441 tggagagacg aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt
    22501 attcagtttg ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc
    22561 aatatcgcag cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac
    22621 attgtcagcc ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta
    22681 atcaacgcca taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc
    22741 gcagcccata tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg
    22801 caggtagctg tccgttggcg aggcgcaatt ttccgccatt ttctccgccc gttgatccca
    22861 gtgatcgggc gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc
    22921 aaaatcaata tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg
    22981 atgaatttgg tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc
    23041 ggtcggtaag ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat
    23101 ggcgttggca tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag
    23161 ctgcgccagc gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc
    23221 atccagtaac aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg
    23281 ttcaccgccg ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc
    23341 cagttgctcc gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatggt
    23401 gggggccagg cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc
    23461 ctgcggcacc caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag
    23521 gctatccagt aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag
    23581 tgtggttttg ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt
    23641 gaaggacacg tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc
    23701 aagcaaagtc actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca
    23761 aaagcggtca gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg
    23821 gtaagcagga gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta
    23881 tgaccggtca acaagcgggc aatgtgcgga acgaccagac cgatccaacc aattatgccc
    23941 gctattgcta ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga
    24001 cgcgtcacgt tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg
    24061 cgccagcgga gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag
    24121 agatcgcgta aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac
    24181 tgggtgtaag gatcggccag cgttttaatt ggcgatatcc ccgctccgca caacgttcct
    24241 aacgcaatgc ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta
    24301 atcaaccaga cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa
    24361 acaatgggta atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg
    24421 cctaaaatat ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct
    24481 cctgccagcg ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag
    24541 aagacaattt gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc
    24601 tgcatatcga gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc
    24661 agcaaaatac atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat
    24721 cactgagtga acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc
    24781 gattgatggc gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc
    24841 ccggcggggc atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg
    24901 caacggcttt cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct
    24961 cctgaaccag aataatatcc ggctgccagc gcaggagatt ttccatggaa acctgtgtca
    25021 ggccgtggcg atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg
    25081 tatgcaacga accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc
    25141 ggaggttagc ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga
    25201 gattggcttg ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact
    25261 gttctgctga ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc
    25321 taacctgccg tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat
    25381 cgggatgtaa cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc
    25441 ccagcctggg taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat
    25501 caaaagagga aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg
    25561 gcgcgcctgc gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa
    25621 aacgattaac tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg
    25681 cctgcgcaaa cgtccgtctg gttatgggca tggtgaacat c
//
LOCUS       DAJTYI010000046        25388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYI010000046 DAJTYI010000000
VERSION     DAJTYI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..233)
                     /locus_tag="OO564_001869"
     CDS             complement(<1..233)
                     /locus_tag="OO564_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6653013.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCK"
     gene            complement(230..1138)
                     /locus_tag="OO564_001870"
     CDS             complement(230..1138)
                     /locus_tag="OO564_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6653014.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1334..2101
                     /locus_tag="OO564_001871"
     CDS             1334..2101
                     /locus_tag="OO564_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6653015.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(2152..2804)
                     /gene="pphB"
                     /locus_tag="OO564_001872"
                     /pseudo
     CDS             complement(2152..2804)
                     /gene="pphB"
                     /locus_tag="OO564_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2910..5471)
                     /gene="mutS"
                     /locus_tag="OO564_001873"
     CDS             complement(2910..5471)
                     /gene="mutS"
                     /locus_tag="OO564_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6653016.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5547..5726
                     /locus_tag="OO564_001874"
     CDS             5547..5726
                     /locus_tag="OO564_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653017.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            5758..6111
                     /locus_tag="OO564_001875"
     CDS             5758..6111
                     /locus_tag="OO564_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6653018.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(6148..8226)
                     /gene="flhA"
                     /locus_tag="OO564_001876"
     CDS             complement(6148..8226)
                     /gene="flhA"
                     /locus_tag="OO564_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6653019.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(8300..9310)
                     /gene="hypE"
                     /locus_tag="OO564_001877"
     CDS             complement(8300..9310)
                     /gene="hypE"
                     /locus_tag="OO564_001877"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6653020.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(9307..10428)
                     /gene="hypD"
                     /locus_tag="OO564_001878"
     CDS             complement(9307..10428)
                     /gene="hypD"
                     /locus_tag="OO564_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6653021.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(10428..10700)
                     /gene="hypC"
                     /locus_tag="OO564_001879"
     CDS             complement(10428..10700)
                     /gene="hypC"
                     /locus_tag="OO564_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6653022.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(10691..11563)
                     /gene="hypB"
                     /locus_tag="OO564_001880"
     CDS             complement(10691..11563)
                     /gene="hypB"
                     /locus_tag="OO564_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6653023.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(11567..11917)
                     /gene="hypA"
                     /locus_tag="OO564_001881"
     CDS             complement(11567..11917)
                     /gene="hypA"
                     /locus_tag="OO564_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6653024.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            12129..12590
                     /gene="hycA"
                     /locus_tag="OO564_001882"
     CDS             12129..12590
                     /gene="hycA"
                     /locus_tag="OO564_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6653025.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            12715..13326
                     /gene="hycB"
                     /locus_tag="OO564_001883"
     CDS             12715..13326
                     /gene="hycB"
                     /locus_tag="OO564_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6653026.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            13323..15199
                     /gene="hycC"
                     /locus_tag="OO564_001884"
                     /pseudo
     CDS             13323..15199
                     /gene="hycC"
                     /locus_tag="OO564_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            15202..16098
                     /gene="hycD"
                     /locus_tag="OO564_001885"
     CDS             15202..16098
                     /gene="hycD"
                     /locus_tag="OO564_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6653027.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            16116..17801
                     /gene="hycE"
                     /locus_tag="OO564_001886"
     CDS             16116..17801
                     /gene="hycE"
                     /locus_tag="OO564_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6653028.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            17811..18353
                     /gene="hycF"
                     /locus_tag="OO564_001887"
     CDS             17811..18353
                     /gene="hycF"
                     /locus_tag="OO564_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6653029.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            18353..19120
                     /gene="hycG"
                     /locus_tag="OO564_001888"
     CDS             18353..19120
                     /gene="hycG"
                     /locus_tag="OO564_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6653030.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            19117..19527
                     /gene="hycH"
                     /locus_tag="OO564_001889"
     CDS             19117..19527
                     /gene="hycH"
                     /locus_tag="OO564_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6653031.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            19520..19990
                     /gene="hycI"
                     /locus_tag="OO564_001890"
     CDS             19520..19990
                     /gene="hycI"
                     /locus_tag="OO564_001890"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6653032.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            20060..20992
                     /locus_tag="OO564_001891"
     CDS             20060..20992
                     /locus_tag="OO564_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653033.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            20976..21287
                     /locus_tag="OO564_001892"
     CDS             20976..21287
                     /locus_tag="OO564_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6653034.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            21284..21703
                     /locus_tag="OO564_001893"
     CDS             21284..21703
                     /locus_tag="OO564_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6653035.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(21818..23242)
                     /gene="ascB"
                     /locus_tag="OO564_001894"
     CDS             complement(21818..23242)
                     /gene="ascB"
                     /locus_tag="OO564_001894"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6653036.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(23251..24699)
                     /gene="ascF"
                     /locus_tag="OO564_001895"
     CDS             complement(23251..24699)
                     /gene="ascF"
                     /locus_tag="OO564_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6653037.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            24959..>25388
                     /gene="ascG"
                     /locus_tag="OO564_001896"
     CDS             24959..>25388
                     /gene="ascG"
                     /locus_tag="OO564_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6653038.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQP"
BASE COUNT         5797 a         6704 c         6848 g         6039 t
ORIGIN      
        1 tgtttgcaac cttgctgttg caaccagctc agagccgcca gcgattgctg tgtggcttca
       61 accaccggac aggagcgcgt tttcaggctg atcaccagtg cgtcgattgc ttccggcatt
      121 ttacctgttg gaacaccgtt aatttgtacc gttggtagac cgttttccac cagaaaactg
      181 gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc caatcttgat catgatttcg
      241 ctcccggtag agtgatgcca gagaaaatct tgataactgc gctatcgtct tctttcccgt
      301 aacccgcgtt actggcgctg gtgaacatat tcaatgctgt tgaggccaat ggcagcggga
      361 agtgcagggc tttggctgta tcggcaacca gaccaagatc cttaacaaaa atatcgacgg
      421 ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat ccggttttcg aacatccagg
      481 aatttccggc ggcattggtc acgacgtcat acatcacatc cagcgggatc cccgcacggg
      541 ctgcaagtgc catcgcttcg gctccggcag caatatgtac gcccgctaac aactggtgaa
      601 taatttttac ggtcgaacct agtcccggtt ctgcacctat gcgataaact tttccggcaa
      661 cggcttccag cacgggtgcc agtcgttcaa aggcaatatc gctaccggag gccatgacag
      721 tcatttcacc gttagcggct tttactgcac caccagaaac tggcgcatcc agcatttcca
      781 gatcgaatcc agccagagcg gtagcaattt cttgcgcatc agcactagcg atagtggaag
      841 aaaccattac tgccgtaccg ggtttcagat gttgtgcaac gcctgtttca ccaaacagca
      901 cctgtttaac ctgggccgca ttgaccacca gcaccagcag agcgtccagt ttttcggcaa
      961 acgtcgcggc gttatcagaa accccgcaag cacctgcctc tttcaacgta gcgcaggcat
     1021 tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg gacacatgac agtgctgctc
     1081 ccattcccat tgaccctaag ccaacgatac cgacatgaaa ctcagatccc gttttcatct
     1141 gctctccttg ttaatttaag tgatattttg tttgacattg tgaatataag cgctgggaga
     1201 taacgatatg gtgagctgat tcacataaat taacattttg tgttatttta tgtgaactaa
     1261 gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt agaatgtcag caggacaaag
     1321 gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc atccttgaga tggtagctga
     1381 aaaaggcatt gtcagtattg ctgaactaac ggacagaatt aatgtgtcac atatgaccat
     1441 tcgtcgggat ttacaaaaac tggagcagca gggagccgtt gtgctggtgt ccggaggcgt
     1501 ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag gtaaaaactg cgctggcaat
     1561 gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt gttcagccgg gaagttgtat
     1621 ctatctggat gcgggaacga ccacgttagc gatagcacag catctgattc acatggagtc
     1681 actgactgtg gtcacaaacg atttcgttat tgcgaactac ttgctcgaca acagtaattg
     1741 cacaattatt cacactggcg gtgcagtgtg tcgggaaaac cgttcctgtg tcggggaagc
     1801 cgctgcgacc atgctgcgca gcctgatgat tgatcaggct tttatttctg catcgtcatg
     1861 gagtgtgcgg gggatttcta cgccagcgga agataaagtc acggtgaaac gggcgattgc
     1921 cagtgctagc cgccagcgag ttttggtctg tgatgcgacg aaatatggtc aggtggcgac
     1981 atggctggcg ttaccattaa gcgagtttga tcagattatt acagacgacg gtttgccgga
     2041 gagtgccagt cgcgcgctgg cgaagcagga tctctctttg ctggtagcga aaaatgaata
     2101 atggcctgca ataacatctg gttactcatg cttcacagaa gaagcatgag actaccttat
     2161 tttataaaat gacagccgcc cacttttcgg cgatccggta tcaatataaa tctggttagc
     2221 gaacgtctga atgttatcaa acatcatatg tccaaatata aaataatcag cgccgtttat
     2281 ttgttgtaac tcgccattaa gcgatttctg cacacgataa acaggccaga gtaattcgct
     2341 ctccgctatt tctttgtcaa agagatattc atcacccgga taatctgcat gtgcgatgac
     2401 atattttatt atgtcgttag tgatttcaat aatatgtgga aggtgatgga atttcagcaa
     2461 cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa accagtcacc accgctggca
     2521 agccacatat tgccatcgcc ggtcgcgaac gcatccagcg ccattgcttc gtggttgcct
     2581 ttaacggaga taaaccaggg ctggtttagc aggcgcagga cgtcaagact ctccggccca
     2641 cgatcaatat tatcgccgac agaaataagt aagtcggttt cggggaaaaa agagagttga
     2701 tgtaagcggg attgtaataa ctgatattca ccatgaatat caccaacgac ccatatatgg
     2761 cgatagtgat gggcattgat tttttgatag cgtgtagatg gcatggtttt accctgtaaa
     2821 ataagctttc ctattataca gggtattttt atttgattcg tcagttgtcg ttaatattcc
     2881 cgatagcaaa agactatcgg gaattgttat tacaccagac tcttcaagcg ataaatccac
     2941 tccagcgcct gacgcggagt cagtgaatcc gggtcgagat tttccagtgc ttcgaccgca
     3001 ggcgaagttt cttctggcac tgacagcaaa gacatctgcg taccatccac ttgcgtcgcg
     3061 gcggcgttcg gcgaaatgct ttccagctca cgcagttttt gccgtgcgcg cttaataacc
     3121 tctttcggta cgcccgccag agcagcaacc gccagaccgt agcttttgct cgccgcgccg
     3181 tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct ccagcgcatc gagatgtacg
     3241 ttggcgacgc cttccatttt ctccggtagc tgggtcagct cgaaatagtg ggtggcaaac
     3301 agcgtcaatg ccttaatctt attcgccaga ttttctgcgc acgcccacgc cagcgacaga
     3361 ccatcgtagg tggacgttcc acgcccgatc tcatccatta acaccagact gtattcggtg
     3421 gcgttatgta aaatattggc ggtttcagtc atctccacca taaaggttga gcgcccggaa
     3481 gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat cgataggtcc aatctcgact
     3541 ttttgtgccg gtacatagct gccgatgtag gccatcagcg caatcagtgc ggtctggcgc
     3601 atataggtac ttttaccgcc catgttcggg ccggtaataa tcaacatacg gcgctgcggc
     3661 gacagattca gcgggttagc gataaatggc tcattcagta cttgttcaac taccggatgg
     3721 cgaccttcgg taatgcgaat gcccggttta tcaatgaagg tcgggcaggt gtagttcagg
     3781 gtataggccc gttccgccag gttaaccagc acgtcgagtt ccgccagcgc gctcgcgctc
     3841 tgttgcaacg cttccagatg cggcaacagc aggtcgaaca gctcttcata aagctgtttt
     3901 tccagtgcca gtgctttgcc ttttgaggtg agaactttat cttcgtactc ttttagctct
     3961 ggaatgatgt agcgctcggc gtttttcagc gtctggcgac gcatgtagtt gatgggtgcc
     4021 agatggcttt gcccacggct gatttgaatg tagtagccgt gcaccgcatt aaagccaact
     4081 tttagcgtgt ccaggccggt acgttcacgc tcgcggactt ccagacgctc cagataatcg
     4141 gtcgcgccgt cagccagcgc gcgccactca tccagctctt cgttatagcc cgatgcgata
     4201 acaccaccgt cgcgtaacag caccggcggt gtgtcgatga ttgctcgctc cagcagatcg
     4261 cgcagctcgg caaactctcc catcttctca cgtagcgcct gtaccggtgc actatcgaca
     4321 gtttctaact gcgcacgcag ctccggcagt tgctggaaag cgtggcgcat acgggccaga
     4381 tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa tacgttccag gtcgccgacc
     4441 tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca atgcgccaat agtttgctgg
     4501 cgctcaagca acacgcgagt atcgcgcact ggcatatgca gccagcgttt cagcatacga
     4561 ctacccatcg gcgtgacggt gcagtcgagc acagaagcca gcgtattttc cgcaccgccc
     4621 gccaggttct gagtaatttc caggttacga cgtgtcgcgg catccataat gatgctgtcc
     4681 tgctcacgtt ccatggtgat agaacgaata tgcggcaggg tcgtgcgttg ggtatctttc
     4741 gcatactgca acagacaacc ggcagcacaa agtccgcgcg gcgcgttctc gacgccaaaa
     4801 ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct ggcgcgcggt gtcgatttca
     4861 aactcccaca gcgggcgacg gcgcaggccg cgacggcctt caatcagcga catctcggcg
     4921 aaatcttctg catacagcag ttccgccgga ttagtgcgtt gcagctctgc cgccatcgtt
     4981 tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc tgatatccag cgtcgcgtag
     5041 ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt tgtcctgacg ctcctgtaac
     5101 agggcttcat cgctgatggt gcctggcgta acgatacgca caactttgcg ctcaaccgga
     5161 cctttgctgg tcgccggatc gccaatttgt tcgcagatgg caacggactc tccctgattc
     5221 accagtttgg cgagatagtt ttccaccgca tggtagggaa tccccgccat cgggatcggc
     5281 tctcccgccg aagcaccgcg tttggtcagt gaaatatcca gcagttgcga cgcgcgtttt
     5341 gcgtcgtcat aaaacagttc ataaaaatca cccatccggt aaaacagcag gatctcggga
     5401 tgctgggctt tcagcttgag atactgctgc atcatgggcg tatgggcgtc gaaattttct
     5461 attgcactca tggggttatg tccggttccc tgatattaaa tggggtgtga tggtttttat
     5521 tttcgttgta atcacataag gcgcacatga taccggagtt aatcatttca ggggaagaag
     5581 cgaacggcga gttcttgatg ctggttccgg gaaagatgct gttttgcagc ttttgcgtga
     5641 tgcttatgaa gcaagtacgc aaaattgtat cgattggcga acccttcttc tttagagtca
     5701 tagtgccctt taagattaag atgtaatata aatgcatctt aatgaggtgg aggcagcatg
     5761 tccggcaagc gtatctctcg tgaaaaactg acgattaaaa aaatgatcga tctttatcaa
     5821 gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg aggcgttgtt tgtttacgcg
     5881 caaaagcggc tggataaatg tgtgttcggc gaagagaaac cagcctgtaa gcagtgtccg
     5941 gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac agattatgcg ttgggccggg
     6001 ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc atctcatcga tgacaagcgt
     6061 ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac ctcgtgaata gtatgccgga
     6121 caggcagatc tgtccggcaa tttgcagtta aatcaatgcc gatttatcaa ttcccagccg
     6181 cttcatccgt gacagcaggg tcgtgcgttt cagccccaga cgttgcgcag cgcctttagg
     6241 cccggcaacc acgctgttgg tttctttcag cacgcgcaca atcaactgat attcatcttc
     6301 gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca ggctccggta aagcaatatc
     6361 tggcaatgac agctgcagca cgttgccgcg tgttagcaat actgcgcgct caatgacgtt
     6421 ttccagttcg cgtacgttac ccggccactc catgttgctc aaggtgcgca gcgtctcggc
     6481 aggaatgctg tcgatattgc gccccagacg acgggcaatt ttgaaggtaa aggctttcgc
     6541 cagcagcgga atatcttccg gacgctcgcg tagtggcggc aggtgaatcg ggaatacgtt
     6601 caggcggtaa tagagatcgc tacggaactc tcggtcggcg accatttttt tcagatcgcg
     6661 gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt ttgttgctgc cgagacgttc
     6721 aaactcctgt tcctgcaata cacgcagcaa cttcggctgt aactccagtg gcatatcgcc
     6781 cacttcgtcg aggaacagag agcttttatc cgccagttca aaacgaccaa tgcgctgggc
     6841 gctggcaccg gtaaaagccc cacgctcatg accaaacaga tcgctttcca gcaatccggc
     6901 aggcatcgcc gcgcagttca ttttgaccat gcggcgatta ttacgcccac tgagattatg
     6961 gatcgcacgg gcaatcagct ctttacccgt gccagtttca ccgaggatca gcacggtact
     7021 gtcactttgc gccaccattt caacttgttt aagcacgctg tacatggctt cgctgcggcc
     7081 aataatctcg ccaaattcac tatcaacatt gttgagttgc tcggtcaggg cgaggttttc
     7141 atcaaccagc cgttctttca gacgatggat ttcctgatag gcgagggcgt tatcgacagc
     7201 gattgccaca cgttcggcaa tctggcgcag caaattcaga ttggtagtgg taaacacttt
     7261 ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg tcgccagaca tcagcggtaa
     7321 caggcacaag gtttgaatct ggttgcccca ggtgtcgaac aacatgcgtt catagggggc
     7381 taaatcgtcc cgctcgtaga gattgatcag cagcatctct ttacttttga acacgcgttc
     7441 ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg gcgggatgtt gtttatcaag
     7501 atagtgagtg gagtagatgt tgagtttgtt tttacggtgg ctgcgtaaga caatgctgat
     7561 atcgtcaatg tcgaaatagt aatggatttc tttggcgact tcgctgacca gttcgtccat
     7621 atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg atgcggaagt tatcgcgctc
     7681 ccggcataac aactcatagt cgacattgtt gacgacgcgg ctctggattt gttcggtgac
     7741 gacagaaacg atctgcgtaa atgtttgcag acgattgaac tctttttcgc tccagggacg
     7801 atcgtcataa cgaataaatt cacagccacc aaaaatatgc cctcccgccg ccagtggcat
     7861 caggcaatag tgaccaaatt tggggtatag cccacctgcg gccagctgcg gccaggtttc
     7921 acaaaattct tcgtaactgc aatgcagcgt atcagggcgc gacaaaatgc tgcgtaccgg
     7981 accgtgtgcc agaacagttt cgtcttcata tttaatgggg gtgtcttttt cacgcgacgc
     8041 gtaataagac gcacgttgag tctgcgcttg ccacaacaca atagccgcgt tgtcggcgag
     8101 cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc gaggccagat cgggctgctg
     8161 caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg agatcactca tcggtgtata
     8221 tgacattttg ttcttccagg aaagcaccgc aaagcggtgc aaaaggtcat tgactaacta
     8281 taggagattt agaattttat tagcatatac gcggaagcgg ttcggcgtgt ggtaaatcga
     8341 gggttcgttt cacgccatac agaccggcaa gacgaacacc tttacgttcc accacttcac
     8401 caatcagcgc cgcgtctttc cccagtggat gggaatgtaa cgctgccagc acttgctctg
     8461 ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt ggcaaagttc agggcgtcca
     8521 gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac cggcagcgct gattcagaaa
     8581 tttcaatacc acaaccgcag gctgccgcga actcatgaac caccgcgttt acaccaccac
     8641 gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg cagcgtctga ataagcggcg
     8701 tcagcaccgc gcagtcgctg accagttcgc catccagccc cagctgctca cgcaggttaa
     8761 ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag caatacatcg cctgcggtta
     8821 gcgtctgtgc gccccagtga atattcgccg gaattgcgcc catgccagcg gtgttgataa
     8881 acagtttatc tgccgcgccg cgctgcacca ctttagtatc gccagtaacg atggcaatgc
     8941 ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc tttcagtgtc tccatcggca
     9001 atccttcttc gaggataaag ccacaggaga gatagcgcgg aatagcgcca ctgaccgcaa
     9061 cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt accgccagga aagaacagcg
     9121 ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc gccttccgct accagctgcg
     9181 ccagatcaag acgtgcctga tcttcctgct ctgccagcca cgggttggca aaggcttcca
     9241 taaacaggct gttgattaat tgctgcatcg cctggccgcc gctgccgtgg gcgagttgga
     9301 tattattcac gcttcactct cctgctgacg atactgatac cacgcggcgc acgctccttc
     9361 ggaggagacc atcagtgcac caaatgcggt ttgaggatta caggtgttac caaacagcgg
     9421 gcattgatgc ggcttacatt tgcccgttaa tacctcacca caacgcgcgc gcgggtcatc
     9481 gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca tcgaatcgtt gataatccgg
     9541 cgtcaggtgc acgccagaag attcaatcac gcctaagccg cgccattcgc tgtcgccgtt
     9601 gacacagaac acatcggcaa tcgcctgttg cgccagcagg ttaccggcat ccggtaccac
     9661 ccgacgatac tgattctcta ccttgctgtg ggccgctatt ttctgctcca ccagcatgac
     9721 cacgccttgc agtagatcaa ggggttcgaa accagcgacc accagcggac gatgaaaatc
     9781 gctggcgata aaattatagg catcagtgcc gataaccata ctaacgtggc ctggcgcgag
     9841 gaacgcgtcg ataccgttat ccggctgttc cagcaaactg cgcagcgtcg ggataagcgt
     9901 aatgtgctgg cagaagaagt aaaaattctg cacatcacgc gctttcgcct gttgcagagt
     9961 gatggcggtg gtcggcatgg tggtttcaaa acctaagccg aagaacacca ctttgcgggt
    10021 tggattctcc tgcgccagtt tcaacgcatc catcggcgag taaacgatgc gcacatcggc
    10081 accgcgtgct tttgcctgca acagcgatcc ctgtttccct ggtacgcgca tggcgtcgcc
    10141 aaaggtacag aagatgactt ccggatggct ggcaatctcc acgcaggtgt cgattctacc
    10201 catcggcagt acgcacaccg ggcaacccgg accgtggata aactcaacgt tttcaggcag
    10261 taactggtcg aggccgaatt taaaaatggc gtgggtatga ccgccacaca cttccataat
    10321 ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca cgttcgcgca gatgctcaat
    10381 taactgcatc acctgttccg gcgcgcgata ttcatcaaca aaacgcatta tttttcctcg
    10441 ccatacaaca gcgcaccgac atccggctca acgtcaaaca tgttttgtaa ggcgtcgaga
    10501 gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa agccaacgtg aaccagtacc
    10561 cactggccca cgcgcggctg accgttttca tcgcagctgc cgactaacgt taaatcgaca
    10621 tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc cgtcaatggt gcggatctgg
    10681 ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt cagccactgg tccatccctt
    10741 cgccgctggt ggcggaaata aggatgattt caatttctgg attgacttcg cgggcgcagg
    10801 cgatgcactt ctcaacgtca aagttgagat acggcaacag gtcaactttg ttgagcagca
    10861 tcagcgaggc ggcggcaaac atatgcggat atttcagcgg tttgtcttca ccttcggtaa
    10921 cggaaagcac cgctactttg tgtttttcac cgagatcgaa gctggccggg catacgaggt
    10981 tgccaacgtt ttcgataaac agaataccgt tatcgtccag tggcagacgc ggtgcggcgt
    11041 cggcaatcat ctgtgcgtca agatggcagc ctttaccggt gttcacctga atcgctggtg
    11101 taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg ctggtcgcct tcaataaccg
    11161 cgcacggaac gctgtctttc aggcgcatta aggtttccgt cagcagggtg gttttaccgg
    11221 aaccagggct ggaaaccagg ttgagcacca gttgcttgcg ggcagcaaag cgcgcgcggt
    11281 tgcgttcagc cagacggttg tttttgtcca gcacgtcaat ttcgacttcc agcatccgac
    11341 gctggctcat gcccggtgtg tgagtgcccg cttcgccatg accgtaatgc aggtcgcctt
    11401 cttcagtctg gctgggggta aattcaggcg ctttaatgcc ggtgattttc atctgcgggc
    11461 gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg agggttatgt tcatcaccct
    11521 cgatatacag gttgccttca ccgcaaccgc atgttgtaca catcgctcac tcctggtcta
    11581 tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat ctgtagcatg tcaccatgac
    11641 actgtggaca gcggcggacg cgctgggtca gtagcgtcac atactgttgg catgtttcac
    11701 accagcattc ggcctcttgt tcttcgaggt gcagtttaca accttccgcc acgctgccgc
    11761 ggcaaaccag atcaaaacaa aaggcaagag agctggtttc gacacaagaa aatgcgccaa
    11821 ttttgagcca gaccccagtt acgcgttttg cgccgtgttt tgcggcctgc tgttcgatca
    11881 attccagtgc ccgttggcag agggttattt cgtgcatatc gcctcccatt aactattgcc
    11941 agctacaagc aataattgcg ccagtgttga ttatccctgc ggtgaataat gtcgatgatg
    12001 tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta gcttaaaaat ctctttaata
    12061 acaataaatt aaaagttggt acaaaaaatg cttaaagctg gcatctctgt taaacgagta
    12121 acctgacaat gactatttgg gaaataagcg agaaagccga ttacatcgca cagcggcatc
    12181 gtcgcctaca ggaccagtgg cacatctact gcaattcgct ggttcagggg atcacgttat
    12241 cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga caaagaactc tgtttcgtcc
    12301 tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc cacaccatcg
    12361 agtattacgt cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag gcgcaactga
    12421 gcattgacgg catgattgat ggcaaggtca acatccgcga tcgcgaacag gttctggaac
    12481 actatctcga aaaaatcgct ggcgtttacg acagcttata caccgccatt gaaaacaatg
    12541 tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc ggcagcatga gctgaggctt
    12601 tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag tgtcgaaaat
    12661 gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg cctggtgaat
    12721 cgttttgtaa ttgccgactc cacgctctgt atcggctgcc acacctgtga ggccgcctgt
    12781 tcagagacgc atcgccagca cggcctgcaa tcaatgccgc gcctgagagt gatgctcaat
    12841 gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag atgcaccctg cgcggtggtc
    12901 tgcccggtta acgccatcac ccgcgttgat ggggccgtgc agttgaatga aagtctgtgc
    12961 gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg caattgaatt ttccggcagc
    13021 cgtccgctgg atattccggc aaacgccaat accccgaaag cgccaccggc accgcctgct
    13081 ccggcgtgtg tcagcacatt gcttgactgg gtgccaggta ttcgcgcgat cgccgtcaaa
    13141 tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg tgcggatgtg cccgactaaa
    13201 gccctgcatc tggtggataa caccgatatc gcccgcgtca gcaaacgtaa gcgtgagctg
    13261 acttttaaca cggactttgg cgatctcacc ttgtttcagc aggctcaaag tggagaggct
    13321 aaatgagcgc aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc gccgctgttc
    13381 tggcatttct cttttctttt caaaaagcgc taagtggctg gatagccgga attggcggcg
    13441 cggttggtag tctgtatacg gcagccgcgg gcttcactgt actgactggc tcggttggcg
    13501 tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat ctccccgctt aacgcgattt
    13561 ggctgattac cctcggtctt tgcggtctgt ttgtcagcct ctataacatt gactggcatc
    13621 gccacgcgca ggtgaagtgc aacggcttgc agatcaatat gttgatggct gccgccgtct
    13681 gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc atggccttgt
    13741 gtgcggtgtt cctcaccagc aacagcaaag agggcaaact gtggtttgcg ctggggcgtc
    13801 ttggcactct gctgctggtg attgcttgct ggctgctgtg gcagcgttat ggcacgctgg
    13861 atctgcgcct gctggatatg cgtatgcaac agctgccgct cggttccgat atctggctgc
    13921 tcggttccga tatctggctg ctcggtgtga ttggctttgg cctgctggct gggattattc
    13981 cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc tgcgccagct gccgcgttgt
    14041 tttctacggt agtcatgaaa attggcctgc tgggcatgtt aaccctgtca ctgctgggcg
    14101 gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct cggcatgatc accgcgttcg
    14161 tcggtggtct gtatgcgttg atggaacaca acatccagcg cctgctggct taccacaccc
    14221 tggaaaatat cggcattatc ctgctggggc tggggctggg gctggggctg gggctggggc
    14281 tggggctggc gtaacgggta tcgcgctcga acaaccggcg ctgattgctc ttggcctggt
    14341 cggtggtctg taccatctgc ttaaccatag cctgttcaaa agcgtactgt tcctcggggc
    14401 ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa aaactcggtg gtattggcaa
    14461 gaaaatgccg gttatctcca tcgccatgtt agtcgggttg atggcaatgg ctgcgctgcc
    14521 gccactgaac gggtttgccg gggaatgggt tatctatcaa tccttcttca aactgagcaa
    14581 tagcggcgcg tttgttgccc gtctgctggg gccgctgctc gctgtgggtc tggcaattac
    14641 cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc gtcacgttcc tcggcgcgcc
    14701 gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc ctgatgagcg taagcgtagt
    14761 ggcactggcg atttgctgcg taattggcgg tgttgctgcg ccgtggctgc tgccgatgct
    14821 ctctgctgct gtacctctgc ctctggagcc tgctaacacc accgtttctc aaccaatgat
    14881 cacattgctg ctgattgcct gcccgctgct gccattcatc attatggcga tttgcaaagg
    14941 cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg tgcggctacg accatgaaaa
    15001 atcaatggtg attaccgctc acggttttgc catgccggtt aaacaggcgt ttgcgccagt
    15061 gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg ccgggctggc agtgcgaagg
    15121 tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg gcggtactgg tggtgattat
    15181 tgtttcacga ggagcctgag aatgagtgtt ttatatccgt taattcaggc gctggtgtta
    15241 tttgccgttg cgccgctgct ctccggtatt acccgcgtgg cgcgcgcccg tctgcataac
    15301 cgtcgcgggc cgggcgtgtt gcaggagtat cgcgacatta tcaaactgct gggccgccag
    15361 agcgttggcc cggatgcctc tggctggatg ttccgcctga cgccgtatgt gatggtgggc
    15421 gtcatgctga ctatcggttc tccgctgccg caactgggcg atttgatcac cttactgtat
    15481 ctcttcgcca tcgcgcgttt cttctttgcc atttccggtc tggataccgg tagcccgttt
    15541 accgctatcg gtgcgagccg tgaagcgatg cttggcgtgc tggtcgaacc gatgttgctg
    15601 cttggtctgt gggttgccgc acaggttgcc ggttccacca acatcagcaa cattaccgac
    15661 accgtttatc actggccgct gagccaaagc atcccgctgg tgctggcgct ctgcgcctgt
    15721 gcgttcgcca cttttatcga aatgggcaaa ctgccgttcg acctggcgga agccgagcag
    15781 gagttgcagg aaggtccgct ctccgaatac agcggtagcg gctttggcgt catgaaatgg
    15841 ggtatcagcc tgaaacagct ggtggtgttg cagatgttcg tcggggtgtt tattccgtgg
    15901 ggacaaatgg aaaccttcac cgtcggtgga ctgctgctgg cgctggtgat tgccatcgtc
    15961 aaactggtgg tcggcgtcct ggttatcgcg ctgttcgaaa acagtatggc ccgtctgcgt
    16021 cttgatatta ctccgcgcat tacctgggct gggtttggct ttgcattttt agcgttcgtc
    16081 tccttgctgg cggcgtgatt aaagagagtt tgagcatgtc tgaagaaaaa ttaggtcaac
    16141 attatctcgc cgcgctgaat gaggcatttc caggcgtcgt gctggaccac gcctggcaga
    16201 ccaaagatca gctgactgtc accgtaaagg tgaactatct gccggaagtg gtggagtttc
    16261 tctactacaa gcagggtggc tggctgtcgg tgctgtttgg taacgacgaa cgcaaactga
    16321 atggtcatta cgccgtttat tacgtgctgt cgatggagaa gggcaccaag tgttgggtaa
    16381 cggttcgcgt cgaagttgac gccaacaaac cggagtatcc gtccgtgacg ccgcgcgttc
    16441 cggcggcggt gtggggcgag cgcgaagtgc gcgatatgta cggtttgatt ccggttggtc
    16501 tgccggatga ctggccggat gaactttatc cgctgcgtaa agacagcatg gattatcgtc
    16561 agcgtccggc accgactacc gatgctgaaa cctacgagtt catcaacgaa ctgggcgaca
    16621 agaaaaacaa cgtcgtgccg attggtccgc tgcacgtcac ttctgacgaa ccgggtcact
    16681 tccgtctgtt cgtcgatggc gaaaacatta tcgacgccga ctaccgtctg ttctacgtcc
    16741 atcgcggtat ggaaaaactg gcggaaaccc gcatgggtta taacgaagtg accttcctct
    16801 ctgaccgtgt gtgcgggatc tgcggctttg cccacagcac cgcctacacc acgtcggtgg
    16861 aaaacgcgat gggtattcag gtgccagaac gcgcgcagat gatccgcgcc attctgctgg
    16921 aggtggaacg cctgcactcg catctgctca acctcggcct cgcctgtcac tttaccgcct
    16981 tcgactccgg ctttatgcag ttcttccgcg tgcgtgaaac ctccatgaaa atggcagaga
    17041 tccttaccgg tgcgcgtaaa acctacggcc tgaacctgat cggcgggatt cgtcacgatc
    17101 tgctgaaaga tgacatgatc cagacccgcc agctggcgca acaaatgcgt cgtgaagtgc
    17161 aggagctggt ggatgtgctg ctgagtacgc cgaacatgga acaacgcacc gtcggcattg
    17221 gtcgtctgga cccggaaatc gcccgcgatt tcagtaacgt tggcccgatg gttcgcgcca
    17281 gcggtctcgc ccgcgatact cgcgccgatc acccgtttgt cggttatggc ctgctgccaa
    17341 tggaagtcca cagcgagcag ggctgcgacg ttatttcccg tctgaaagtg cgaatcaacg
    17401 aggtctatac cgcgctgaat atgatcgact acggtctgga taacctgccg ggcggtccgc
    17461 tgatggtgga aggctttacc tacattccgc accgtttcgc gctgggcttt gccgaagcgc
    17521 cgcgcggcga cgatatccac tggagcatga ccggcgacaa ccagaagctg taccgctggc
    17581 gctgccgtgc cgcgacctac gcgaactggc cgaccctgcg ctacatgctg cgtggcaata
    17641 ccgtttccga tgcgccgctg attatcggta gcctggaccc ttgctactcc tgtaccgacc
    17701 gcatgaccgt agtcgatgtg cgtaagaaga agagcaaagt ggtgccgtac aaagaactcg
    17761 agcgttacag cattgagcgt aaaaactcgc cgctgaaata aggaatcgcc atgtttacct
    17821 ttatcaaaaa agtcatcaaa accggcacgg cgacctcgtc ttatccgctg gagccgattg
    17881 cggttgataa aaacttccgt ggtaagccag agcagaaccc gcagcagtgc atcggctgcg
    17941 cggcttgcgt caatgcctgc ccgtcaaacg ccttaacggt tgaaactgac ctcgccacag
    18001 gagagctggc gtgggagttt aatctcgggc gctgcatctt ctgtggacgc tgcgaagaag
    18061 tttgcccgac ggcggcgatc aaactgtcgc aagagtacga actggcggtg tggaagaaag
    18121 aaaacttcct gcaacagtcc cgcttcgcgc tgtgcaactg ccgcgtctgc aatcgtcctt
    18181 tcgccgtaca gaaagagatc gactacgcca ttgcgctgct taagcacaat ggtgacagcc
    18241 gcgcggaaaa ccaccgcgaa agctttgaga cttgcccgga atgtaagcgc cagaaatgcc
    18301 tggtgccgtc cgaccgtatt gaactgactc gccatatgaa agaggccatc tgatgagcaa
    18361 tttattaggc ccccgtgacg ccaacggcat tccggtcccg atgacggtgg atgaatccat
    18421 cgccagcatg aaggcgtcgt tactgaaaaa aatcaaacgt tctgcctatg tttaccgcgt
    18481 ggactgcggc ggctgcaacg gctgcgaaat cgaaattttc gccacgcttt ctccgctgtt
    18541 cgatgcagaa cgcttcggca ttaaagtcgt tccttcaccg cgtcatgcgg atattttact
    18601 gtttaccggc gcggtcaccc gtgcaatgcg atcccctgcg ctgcgtgcgt ggcagtccgc
    18661 gccggacccg aaaatctgta tctcatacgg tgcctgcggt aacagcggcg ggatcttaca
    18721 cgatctctac tgcgtgtggg gcggtacgga taaaattgtc cctgtggatg tttatatccc
    18781 tggctgcccg ccaacgcctg ccgccacgct gtacggcttt gcaatggcgc tcggcctgct
    18841 ggagcagaaa attcacgccc gtgggccggg tgaactggat gaacaaccgg cggagatcct
    18901 gcatggcgat atggtgcagc cgctgcgcgt gaaagtggat cgcgaagcac gtcgcctggc
    18961 gggttatcgt tacggtcgtc agattgccga tgattacctt acacagttag ggcagggcga
    19021 agaacaggtt gcacgctggc tggaagcgga aaacgatccg cgtctgaacg agattgtcag
    19081 ccatctgaat catgttgttg aagaggcgcg tatccgatga gtgaaaaggt ggtgttcagt
    19141 caactgagcc gtaaatttat tgatgagaac gatgccacgc ccgccgaggc gcagcaggtg
    19201 gtctattaca gcctggcgat tggtcaccac cttggggtta tcgattgcct ggaagcggcg
    19261 ctcacctgcc catgggatga atatctggca tggattgcca ctctggaggc gggcagcgaa
    19321 gcccgccaca aaatggaagg cgtaccgaaa tatggcgaga tcgtcatcga cattaaccat
    19381 gtgccgatgc tggccaacgc attcgataaa gcccgggcag cgcaaacttc gcagcagcag
    19441 gaatggagta caatgctgtt aagtatgctg catgatattc atcaggaaaa cgccatctat
    19501 ttgatggtga ggagactgcg tgactgacgt tttactctgt gttggcaata gcatgatggg
    19561 cgatgatggc gctggcccgc tgctggcgga aaagtgcgcc gccgcgccga aaggtaactg
    19621 ggtggtgatt gacggcggta gtgcaccgga aaacgacatc gtcgccatcc gtgaactgcg
    19681 cccgacacga ctgctgattg tcgacgccac ggatatgggg ctaaaccccg gcgaaatccg
    19741 catcatcgac ccggatgata tcgccgagat gtttatgatg actacccata acatgccgtt
    19801 gaattacctt atcgaccagt taaaagaaga cattggtgaa gtgattttcc tcggcattca
    19861 gccggatatc gtcggctttt actacccgat gacccagccg attaaagatg cggtagaaac
    19921 cgtttatcaa cgactggaag gctgggaagg aaatggtgga tttgcgcagt tagcggtgga
    19981 agaagaataa ttctccatca gggaattcag ggaattcagg gaattcaggg aattcaggga
    20041 attgaacatc agaacaggga tgtgcgcttt tttattgagc ttagtgctgc ctgcacaggc
    20101 caccagcttt accgaatatc tgccaatgag tgacagcgaa tacgcgcaaa agcgggcgct
    20161 gaaacctctc ctgaccatgc cttatgatgc cgagcaaacc tggcatttta ggaaagtggg
    20221 cgttgctggc gtgacgcttg agaagatgcc aaatgaagaa gattactggc aattaaccgc
    20281 taaagaccgc gccggtaaat cctggactgt gcctgtgggt atgctggcaa acatagcggg
    20341 caaagggcaa ctctatcgcg ccgatcttga tcgtaacggc attcaggatc tggttatctg
    20401 gttaccaagt accggtaatg gtctggctcc ttatgcacat ttaattctta tgacgttcac
    20461 gcgcgaaggc cgtccctgtg tctttgaacc caggggattc tacaccgcaa gcaaaactgg
    20521 cgtagatgat ttgctggatt tgcaaggcaa tggtcacacg caattgctgg atatgcagtt
    20581 taattctggt tactggataa ccagtctgta tcaggtaaaa gacgccaaat ggcagcgtgt
    20641 tcacggctgg tttgacaagt taagctatcc cgcgctcacg cgttttactt atacccccaa
    20701 ccgtaaactc gtcctcaaac ccatcgccgg gcgcgacccg caaacggaag atctggcgca
    20761 gacgcagcgt tgcttgataa agggggatgt gcttgagggg ggaaccagaa ctgacttttt
    20821 gcagagtggc tatgaggatt ttgggggtaa ttatatcgcg cacaagtgtt tacatattgt
    20881 gaacttggat ttgatgagct atagtttccg aacacataag gatagaactc gcagatgtgg
    20941 ctgtttactt actataagga agttaaaact taaaaatgca catcatttct aaagcgccat
    21001 ttgaagagtc tgcgcgtaaa tatcctaacg acgctctggc tcttcaggca ttgtatcggg
    21061 ttattaagga aacggatttt tctacgccag aagagatgcg aacggcgttt cccaacctcg
    21121 acaattttaa atatcgcaac aaatggtacg ttctggatgt aggtgggaac aacctagggg
    21181 tgattgccta cataaacttt gttaataagc ggttctttgt gaagcacata actaatcacg
    21241 ccgagtatga caaactgaca cgttactaca gagagaacaa agaatgactg ctaatgctgc
    21301 acacgctgtt aaagcaacaa gagaactggt caatgcagtc ccctttctgg gaggtagtga
    21361 ctcagaagat gattatcgtg aagcactgga actggtcgaa tatttgattg aagaagatga
    21421 caccaatcca cttatcgact ttctggcgag ccgcattgca gaatatgaaa acaacaacca
    21481 agagtttgca gagttcgaca aagccgttgc ggcaatgcct gttggcgtgg cgttacttcg
    21541 cacgctgatt gatcaacata atctgaccta tgcggatttg aagaacgaaa tcggctctaa
    21601 atccctggtc agtcagattt tgtctggaca acgatccctg acgatatccc acattaaggc
    21661 actttcagcc cgatttggcg ttaagccgga atggtttctt tgatttttga ctaatgccaa
    21721 atgctaaatt gcccgatgcg ctgcgcttat cgggcctaca tgtcgcacga actatgtagg
    21781 ccggataagg cgttcacgcc gcatccggca ctgttactta ctctaaatct tccccattac
    21841 tggcaatcac ttttttatac caccagaacg atttcttacg tgtgcgcgtc agcgtgccgt
    21901 tgcctgcgtc gtcacggtct acgtagacaa aaccgtagcg tttgctcatt tcacccgtag
    21961 aggcggaaac taaatcaata cagccccatg tggtgtagcc catcagcgga atgccatctg
    22021 caatcgcttc gcctattgcg cggatatgtt cgcgtaaata gctgatgcga tagtcatcgt
    22081 taatctcgcc attggcagca agttcatctt ttgcgccgag gccgttttcc accagaaaca
    22141 gcggcttctg ataacgatcg tacatcatat tcatggtgat acgcagaccg agtggatcaa
    22201 ttccccagcc ccagtcgctc acttgcagat acgggttacg cagcgatttc accacgttcg
    22261 ccgcactgct gttgttggcg ttcatctccg ccgaggcgca gcgcgaggcg taatagctga
    22321 aagagacaaa atcgacggtg tttttcagga tctcatcatc gcccagcgct ttatctatgg
    22381 ttaccccttt ttcgcgaaat acgcgggcag agtaagccgg atacgcgccg cgcgcctgca
    22441 catcgataaa aaacaggttt tcccgatctt tctccagcgc cgcccagaca tcttccggtt
    22501 tgcagctgta agggtagaag ttaccgcccg ccagcataca gcccacctga ttttgcgggt
    22561 taacctcatg ggcgattttg gtggctagcg cactggcaac cagctggtga tgcgcggcct
    22621 gatatttcac ctgatcctga ttttcacctt cttcaaacac cagacccgcg ccggagaacg
    22681 ggctatgcaa cataatgttg atttcattga aggttagcca gtatttcacc agaccatcaa
    22741 atgcttcaaa gcaggttctg gcgtagcggc tgaaaaactc caccagcttg cggttacgcc
    22801 aggagccata ttcggtgacc agatgcatcg gcacatcgaa gtggcacaac gtgaccagcg
    22861 gttcgatacc gtacttttta cactcttcaa agacaggacg ataaaaagca atgccctgct
    22921 gattgggcgt gatttcatcg tcctgcggaa agagacggct ccaggcaatt gaggtacgga
    22981 aaaccttgaa tcccatctct gccatcaggg cgatatcttc tttataacga tgataaaaat
    23041 ccgtcgcctc atggctggga taaaactcgt catcgcgcaa ctgaaaacgt ttttccagcc
    23101 ccagtttcac cgccattcga tgctcgccgt gtgggatcat atcgacggtg gtcagccctt
    23161 ttccgccttc acggaacgca ccttcagact ggttggcggc aagcgcgccg ccccataaaa
    23221 aaccttctgg aaatactgac attttcatcc tcaattaaga cttacttctt tggcgacggt
    23281 cggttgatgc tttcgcgcct gggcagccgc ttcctcaaca ggaatatcct caaagccgag
    23341 caacagtgtg aggataaacg acagcaccac cgccagcgcc atgaccgcga acacccagac
    23401 gatgctcatt ggattcgccg gatcgaagaa ttgcacgctg gtaaatagcc ccggcgctgc
    23461 cattgagtgg ctggcaagcc ccgccatacc ggcaaccgcg ccgcaaataa aaccgctgat
    23521 aagactggcg ataagcggac gtttcaggcg gatcgccacg ccgtataacg ccggttcgga
    23581 aatccccgcc ataatggctg atgccgccgc agccagcgcc gtctggcgca gttccgggtt
    23641 tttcgttttc cacggcaccg ccagtgatga accgcccagc gacaggttag cgccgatctc
    23701 tgatggcatg accattcctt ctttgccggt ttcggcaatg gtctgaatga tggttggcgt
    23761 aaagacgcgg tgcatcccgg tcattaccag cagaggccac agcgcgccca taatggcgac
    23821 tgaaagccag cccagataac catgaatggt gtaaaccagc gccgaaatgg cgctaccgat
    23881 ccagatacca atcgggccaa tcagcaggat tgccagcggt gcggcaatca acacaatcag
    23941 cattggcttg aggaagtttt tggtcaccgc cggtgtaatg cgatccaccc agcgttcgat
    24001 atatgacagg caccaggtca tcaccagcgc cgggatcacc gtgtaggtgt atttcaccgc
    24061 ggtgaccggg atcagggcaa attcgacatg ttcaccctgg gccgctttcg ccattaattc
    24121 aataaaactc ggatgtacca gcacacccgc gatggcaatc gccagcgaca tattggtttt
    24181 aaatttgatg gcggcagatg ccgcgaccat tagcggcagg aagaagaaag caccgtcacc
    24241 aatcacattc agaatggtta aggtcggcga tccttttgtc agcacgccgc tcatctcgag
    24301 gatcatcgcc agcagtttga ccatcgatcc gccgataatc gccgggatca gcggtgacat
    24361 ggtgccgatc agcgcatcga ggatccccgc accaatgcga cgcagcgtga gtttgggttt
    24421 acccacgggc tgtgcgggct gcatatctcc cggcagcagg ctgacgactt cctgaaaggc
    24481 ctgcgaaacc gtattgccga taattacctg acactggttg tcactacgta ccacgccgag
    24541 cacgccgggg atggttttta acgtcgggct gtcgataagc tggtcatctt tgataacaaa
    24601 gcgcaaacgc gtcatacagt gcgtgactgc cgagatgtta tcaacgccgc ccagtgccgc
    24661 tatcaccgag cgtgccagcg ccgcataatt tttggccatc ggattttatc ctgttatcag
    24721 tagggtagat gtagttgtta tcagtcgacg tgacggaaaa ataccgatgc tgaaacggaa
    24781 ttaagaaacc ggtttcacaa aatcatgaat agttgtcact aaataagcaa gagagttaat
    24841 tgttcatttt ttatatttgt gaaaccggtc acctgaatat ttttcccgcc gattgcgcaa
    24901 ggacaatcgg caaacggtgc agtacactgc gttgattgcg tgtaaaggga tgaaaatgat
    24961 gacgacgatg ctggaagtgg cgaagcgcgc cggggtttca aaagcgaccg tttcccgcgt
    25021 gctttcaggt aatggctacg tcagccagga gactaaagat cgcgtgtttc aggcggtaga
    25081 agagagcagt taccgcccaa atttgctggc ccgcaatctg tcggcgaaga gtactcagac
    25141 gctggggctg gtagtgacca acacgcttta tcacggcatt tattttagtg aattactgtt
    25201 tcatgccgcg cgaatggcgg aagagaaagg gcgacagttg ctattggcag atggtaaaca
    25261 cagcgccgaa gaagagcgcc aggcgattca gtatctgctg gatttgcgct gcgacgccat
    25321 tatgatttac ccgcgttttt taagcgtgga tgagattgat gacatcattg acgcgcacag
    25381 tcagccga
//
LOCUS       DAJTYI010000047        24892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYI010000047 DAJTYI010000000
VERSION     DAJTYI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..251
                     /locus_tag="OO564_001897"
     CDS             <1..251
                     /locus_tag="OO564_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6653039.1"
                     /translation="QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIY
                     RNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(261..1688)
                     /locus_tag="OO564_001898"
     CDS             complement(261..1688)
                     /locus_tag="OO564_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6653040.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1685..2392)
                     /locus_tag="OO564_001899"
     CDS             complement(1685..2392)
                     /locus_tag="OO564_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6653041.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2550..3533
                     /locus_tag="OO564_001900"
     CDS             2550..3533
                     /locus_tag="OO564_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6653042.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3603..4085)
                     /locus_tag="OO564_001901"
     CDS             complement(3603..4085)
                     /locus_tag="OO564_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6653043.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4320..6902
                     /gene="leuS"
                     /locus_tag="OO564_001902"
     CDS             4320..6902
                     /gene="leuS"
                     /locus_tag="OO564_001902"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6653044.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6917..7498
                     /gene="lptE"
                     /locus_tag="OO564_001903"
     CDS             6917..7498
                     /gene="lptE"
                     /locus_tag="OO564_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6653045.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7498..8529
                     /gene="holA"
                     /locus_tag="OO564_001904"
     CDS             7498..8529
                     /gene="holA"
                     /locus_tag="OO564_001904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6653046.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8531..9172
                     /gene="nadD"
                     /locus_tag="OO564_001905"
     CDS             8531..9172
                     /gene="nadD"
                     /locus_tag="OO564_001905"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6653047.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9196..9807
                     /locus_tag="OO564_001906"
     CDS             9196..9807
                     /locus_tag="OO564_001906"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6653048.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10067..10384
                     /gene="rsfS"
                     /locus_tag="OO564_001907"
                     /gene_synonym="rsfA"
     CDS             10067..10384
                     /gene="rsfS"
                     /locus_tag="OO564_001907"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6653049.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10388..10855
                     /gene="rlmH"
                     /locus_tag="OO564_001908"
     CDS             10388..10855
                     /gene="rlmH"
                     /locus_tag="OO564_001908"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6653050.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10886..12787
                     /gene="mrdA"
                     /locus_tag="OO564_001909"
     CDS             10886..12787
                     /gene="mrdA"
                     /locus_tag="OO564_001909"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6653051.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12790..13902
                     /gene="mrdB"
                     /locus_tag="OO564_001910"
                     /gene_synonym="rodA"
     CDS             12790..13902
                     /gene="mrdB"
                     /locus_tag="OO564_001910"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6653052.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13913..15001
                     /gene="rlpA"
                     /locus_tag="OO564_001911"
     CDS             13913..15001
                     /gene="rlpA"
                     /locus_tag="OO564_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6653053.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15140..16351
                     /gene="dacA"
                     /locus_tag="OO564_001912"
     CDS             15140..16351
                     /gene="dacA"
                     /locus_tag="OO564_001912"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6653054.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16462..16725
                     /locus_tag="OO564_001913"
     CDS             16462..16725
                     /locus_tag="OO564_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6653055.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16826..17467
                     /gene="lipB"
                     /locus_tag="OO564_001914"
     CDS             16826..17467
                     /gene="lipB"
                     /locus_tag="OO564_001914"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6653056.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17726..18679
                     /locus_tag="OO564_001915"
     CDS             17726..18679
                     /locus_tag="OO564_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6653057.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18888..19853
                     /gene="lipA"
                     /locus_tag="OO564_001916"
     CDS             18888..19853
                     /gene="lipA"
                     /locus_tag="OO564_001916"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6653058.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19954..20157)
                     /gene="tatE"
                     /locus_tag="OO564_001917"
     CDS             complement(19954..20157)
                     /gene="tatE"
                     /locus_tag="OO564_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6653059.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20286..21074)
                     /locus_tag="OO564_001918"
     CDS             complement(20286..21074)
                     /locus_tag="OO564_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6653060.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21167..21550
                     /gene="crcB"
                     /locus_tag="OO564_001919"
     CDS             21167..21550
                     /gene="crcB"
                     /locus_tag="OO564_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6653061.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21604..21813)
                     /gene="cspE"
                     /locus_tag="OO564_001920"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21604..21813)
                     /gene="cspE"
                     /locus_tag="OO564_001920"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6653062.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21988..22548)
                     /gene="pagP"
                     /locus_tag="OO564_001921"
     CDS             complement(21988..22548)
                     /gene="pagP"
                     /locus_tag="OO564_001921"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6653063.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23137..24522
                     /gene="dcuC"
                     /locus_tag="OO564_001922"
     CDS             23137..24522
                     /gene="dcuC"
                     /locus_tag="OO564_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6653064.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24563..>24892)
                     /locus_tag="OO564_001923"
     CDS             complement(24563..>24892)
                     /locus_tag="OO564_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6653065.1"
                     /translation="LTRFRQCKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDP
                     LTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRP
                     QRIYHSG"
BASE COUNT         6329 a         6207 c         6138 g         6218 t
ORIGIN      
        1 cacaaagcaa actggggtgg atctacctga aaggcctggg agtcaaaccc gatacccgta
       61 aagcaattct ctggtataag gaagcagctg aacaagggta tgctcatgct caatatactt
      121 tgggtttgat ctacagaaat ggcactggta ttaatgttaa ccattatgaa tctcaaaaat
      181 ggttaaaact ggccgccaaa caacattaca aaaatgcgga aagattactt gccggacttc
      241 ccgcacatta acgtcgactg tcacccgaag agatggagta ttttccctat caaaccggca
      301 aagataaaaa tgtagaaaat aattttgatg acgcccagac agcccagccc gccatcgtca
      361 acgggcattg gctgcggttg cggctccggc tcgacggttt cctgaccggt aaaattcgcc
      421 gcctgaatta ataatcgacg aataattaat gcttccagtg gacaacgatc ttctgcatca
      481 aggcggctca caatcggcat taatgattct tctgtcggtt ggctggcctg cactaattgt
      541 ttggcaacta aatataatgg ccataaggga tgttctgtat cacgcgtgag tgagatccgc
      601 ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac gccccagcgc agagagtttt
      661 tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa
      721 agtagcggtt ttccctgatc gttaaccaga tcacgcgctt caatcatcat caccactaat
      781 gggtaataat cgacccgatg ctgacggcac cattgcaaca ttaaagcatc cgcctgggtg
      841 gagggatact cgcgccagta gtaacaaagt tctgccagca ggctttcgcc ttctccacaa
      901 tagacgcgtt gtgcgtattc ataataacgc gcatcttcat tatcaggttc agcttcccgc
      961 cacgaccggg cgtaatccag gagttcgggg attcccaggc ggcttgcgct ataccagcgg
     1021 agtaaatttc tgtgcagttt tgcatcatcg gggataatca ctgcgcgatg ttgcgctaaa
     1081 tacaatgtca gccattctgg atgcgatgac caaatccgag cgaacatttg gtaaaaatcg
     1141 atggtgtcat tttgcacggc aacgggcaga tgcagaatgg tctggaagtt aaaaacattc
     1201 cccttcgcaa tggcatataa aaaggattcc agttcttctt cgtctatttc cccgacgttt
     1261 tcctcctgca accaccgcaa tctttctgct aataacacca cgcactcgaa ggataaatgg
     1321 gcagtgttca tggcgattgt gcacagcgac cagcgtaatt catcaatctc ttccatcgag
     1381 caataattta attgctgaat gaatcgctgc caggcggagg gcagaaaacg ttcgctatca
     1441 gaggcaagta acgcgcgaaa ggcgagcaga atttcatgtt ctgctacctc atattcttct
     1501 ggttgccaaa cagatttagc aggcgactgc gcaatccgta gcgcctcctc atacgcctga
     1561 cgaagttgtt taaaaccctg cggatcggtt tctggatgaa aggacggtaa cagcgccaga
     1621 taagcgcggc ggataatatc gacgtcagtc gtttcctcta tatcgaggat cttccagcaa
     1681 tttttcataa gtcgctcata tccttaagcg tttccgggca tgtggggtag tcagccagtg
     1741 gttgccagga caaggtaggg taatacgcat catcactggc aaataattga taaaacatat
     1801 cattgccgga atcgtcgtat tctttacgcg ctaagaattc gcgtaataaa ggcagatgat
     1861 catcttcggg agactgccaa tattgccgac caaaggtata accggcaaag tattgccgcc
     1921 agttactata ataacgtaga gcccgtagat gaatacgcga caggatccat agactttctt
     1981 cttcactcaa ccagttatta agtacgccca ttcgacttaa aaaacccatc cggacataat
     2041 cccatgcttt aattcctccc tcaccacagc attcggctgt gctggcgaca aattgcgcat
     2101 aagcctgacc ttgctcattt aattcagcaa gatagtcacg ccattcacat ggagaataac
     2161 gaaaccagcg gtgataaaac cctgccaacc gagcggcatg accgttctcg atcattcggt
     2221 gaattgtgcg cagcaggtca tcacggtcat caattcccca tgaggaagaa aggtcaaaaa
     2281 agcatgtacc taaatacatt ttgggggatg aatgcgaagg taaacagcat tcattgcgta
     2341 tttccatcgg agtggaaagt gcaaacaata ttttttgcga ccccatgtcc atataattta
     2401 tccttaaaca ttcatttaat gaatgcaaac tacatttcga gactcagtta tttatacttt
     2461 tctgacgcaa cataatacgc ataaaataag gattacgcaa aaatggaaca cataaaaata
     2521 taatatattt tatctacaac cataaggaaa tgataataat ttttacgtca agttgctgcg
     2581 ataatttatc aatagatgag attatcgaac gtgctgaaaa aggcgattgc gaggctcaat
     2641 atattgttgg gttttattat aatcgcgata gcgcaattga ctctccggac gacgaaaagg
     2701 ccttttactg gctgaagctg gccgctgagc aaggtcattg tgaagcacag tattccttag
     2761 ggcaaaagta taccgaggat aaaagccgtc ataaagataa tgagcaagcc atcttctggc
     2821 tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa cgcccttggc tggatactgg
     2881 atcgtggaga agaccccaac tataaagaag cggttacctg gtatcagata gccgcggaga
     2941 gcggaatgtc ttatgcgcaa aataatcttg gatggatgta cagaaatggc aacggagtcg
     3001 cacaagacta tacgctggca tttttctggt acaaacaagc tgcattacaa ggccatagtt
     3061 acgcacaaga caatctggcc gatctttata aagacggaga aggcgttgct caaaacaaga
     3121 cactcgccgc attctggtat ttgaaaagcg cacagcaggg taatcggcac gcccagtttc
     3181 aaattgcatg ggattataac gctggcgaag gggtggacca ggactataag caagcgatgt
     3241 actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc ttacgtcaac atcggttata
     3301 tgtataaaca cggacaaggc gttgagaaag attatcaggc tgcctttgaa tggtttacga
     3361 aagccgctga atgcaatgac gccactgcct ggtataacct ggccattatg tatcactacg
     3421 gagaaggaag acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat
     3481 ccggaaccag agatgtcagt caggaaattc gtgagactga agctttactg tagaaataaa
     3541 aacaagggcc ggggaatttc attctgccca tattctcttt attgctatgc gaaatcagac
     3601 tattacggct caaacgggcg tctctggaac tggtcataac cacatttcgg gcatagcgtc
     3661 agcacttccg gtgtgtagat cgggagatgg aagtgacatt tctcgcagac cagatttccc
     3721 agcccgacca cttctccgct gtgataaacc ccatgatgat tgaggtcctg gaaaacttcg
     3781 cgccattcaa gctgcgtttt atcggtgatg tctgccagct cctgccacaa gctttcttta
     3841 atcacccgca taaagacgct gtcagattct tctttcaggc tctcttcata gctcatggcg
     3901 aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgatctcggt tcgcgttaac
     3961 tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg
     4021 ccattgcgca ggcgttcgtt cagtgacgcg accagttcac ggtaatattg agcaaccttg
     4081 ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc tttgttcctt
     4141 gctttgctaa tacgcccaca aactctgtaa attaatgtac gaataatccg ggcatcattt
     4201 caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc tatgcggatc
     4261 tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca ctggctgcca
     4321 tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga
     4381 agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ctgtctatgc
     4441 ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc atcggtgacg
     4501 tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc ggctgggacg
     4561 cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga
     4621 cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt ggttatgact
     4681 ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag aaattcttca
     4741 ccgagctgta taaaaaaggc ctggtatata aaaagacttc tgcggtcaac tggtgcccga
     4801 acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata
     4861 ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct tacgctgacg
     4921 agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa accatgcagc
     4981 gtaactggat cggtcgttcc gaaggcgtgg agatcacctt caacgttaac gactatgaca
     5041 acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg
     5101 tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct
     5161 ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcgacg ctggagaaaa
     5221 aaggcgtcga tactggcttt aaagctgttc acccattaac gggcgaagaa attcccgttt
     5281 gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg gttccgggtc
     5341 acgaccagcg cgactacgag tttgcctcta aatacggcct gaacatcaaa ccggttatcc
     5401 tggcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc
     5461 tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac gccatcgccg
     5521 ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg
     5581 gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg gaagacggta
     5641 ccgtaatgcc aaccccggac gaccagctgc cggtgatcct gccggaagat gtggtgatgg
     5701 acggcattac cagcccgatt aaagcagatc cggagtgggc aaaaactacc gttaacggta
     5761 tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc tggtactatg
     5821 cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg gctaactact
     5881 ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac ctgctctact
     5941 tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac gaaccagcga
     6001 aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg
     6061 gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag aaaggccgta
     6121 tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg agcaaaatgt
     6181 ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac ggcgcggaca
     6241 ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa tggcaggaat
     6301 ccggtgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg tacgagcaca
     6361 cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct gactgaagat cagaaagcgc
     6421 tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc cgtcgtcaga
     6481 ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg aaagcaccga
     6541 ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt gtccgtatgc
     6601 tcaacccgtt caccccgcac atctgcttca cgctgtggca ggaactcaaa ggcgaaggcg
     6661 atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa gactccacgc
     6721 tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat caccgttccg gtggacgcaa
     6781 cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg
     6841 gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg gtcgttggct
     6901 aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg cggtgttaat
     6961 caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca ctatgaaggt
     7021 catgatccta gactcaggcg atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt
     7081 acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg ttccatccct
     7141 gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta acggtcaaac
     7201 agcagagtat cagatgatca tgacggtgaa tgcgaccgtg ttgatccccg gccgtgatat
     7261 ctacccgatt agcgccaaag tcttccgttc attcttcgat aacccgcaaa tggcgttagc
     7321 gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct
     7381 gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag aacagacgtc
     7441 gaccacaacg gatactccgg caacgcctgc acgcgtctcc accatgctgg gtaactgatg
     7501 attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg cgcggcgtat
     7561 cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt tcgccaggta
     7621 gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa cactgactgg
     7681 aatgcgatct tttcgttatg ccaggctatg agtctgtttg ccagtcgaca aacgctattg
     7741 ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg aacaacttct cacactcacc
     7801 ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag caaagcgcaa
     7861 gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca
     7921 ccggagcagg ctcagcttcc ccgctgggtt gctgtgcgcg caaaacagct caacttagaa
     7981 ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct gctggcgctg
     8041 gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac attaccgcgc
     8101 gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt tgatgctttg
     8161 ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc aactgcgtct ggaaggcagc
     8221 gagccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt taacctgaaa
     8281 cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt atggcagaac
     8341 cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt acgtcaggcc
     8401 gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca gtcagtgtgg
     8461 gcagagctgg aagggttatc tcttctgttg tgccataaac ccctggcgga cgtatttatc
     8521 gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc cggtgcacta
     8581 tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc gggtcacaat
     8641 catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa
     8701 acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg aacgcgagct
     8761 aaagcgcaat gccccctctt acactgcaca aacactgaaa gagtggcggc aggaacaagg
     8821 accggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct ttccgacctg
     8881 gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc gtccaggtta
     8941 cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt tgacacataa
     9001 cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa cgccgtggtt
     9061 taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt
     9121 attgccggaa ccggtattga cttacattaa ccaacaaggc ttgtatcgct gatacctgtc
     9181 gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca agcgaatgtc
     9241 gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat tgagcaagcg
     9301 caaaatctgc atacactgct agatgatgtt tcctttgacc tggttttatg cagtgaactg
     9361 gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc cgttcatatc
     9421 atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca tcgcgacctc
     9481 atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca tgctatcccc
     9541 acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat cgcaaggctt
     9601 agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt actgagtctg
     9661 ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg cgttgaccag
     9721 gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc
     9781 cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa gccgcaacga
     9841 ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc gccagacacg
     9901 actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg gtgggatgat
     9961 agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct ggttcaggta
    10021 tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc agggtaaagc
    10081 actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt
    10141 agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg gtacgtccag
    10201 ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt
    10261 accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt
    10321 gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa aactctggag
    10381 ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact gggtacaaac
    10441 cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc tgattgaaat
    10501 tccggccggc aaacgcggca agaatgcgga catcaagcgc atactcgaca aagagggtga
    10561 gcagatgttg gcggccgcag gcaaaaaccg cattgtcatc ctcgatattc caggcaagcc
    10621 ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt
    10681 aagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg cggctgagca
    10741 gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga
    10801 gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg agtgataagg
    10861 gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg actatacggc
    10921 agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt tgctgctgac
    10981 cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg actaccagac
    11041 ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg gcattatcta
    11101 cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag aaatgatgcc
    11161 ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg tagatctgac
    11221 cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat
    11281 tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca atcagtaccg
    11341 ttttccgggt gtcgaagtta aaggctataa acgtcgttac tatccttacg gttcggcgtt
    11401 gacccacgtc atcggatatg tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa
    11461 tgacggcaaa ctggccaact atgcggcaac gcatgatatt ggtaagctgg gcattgagcg
    11521 ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag ttaacaaccg
    11581 tgggcgtgtt atccgccagt taaaagaagt accaccgcaa gccggacacg atatttacct
    11641 gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta gccgcgcagc
    11701 tgtggttgtt accgatccgc gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta
    11761 tgacccaaac ctgtttgttg acggtatctc cagcaaagat tattccgcct tgctgaacga
    11821 cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg cgtctacagt
    11881 taaaccctat gtggcggttt cggcattaag cgccggggtg atcacgcgca atacgacgct
    11941 gtttgaccca ggctggtggc aactaccagg gtcggaaaaa cgttatcgtg actggaaaaa
    12001 atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg ataccttctt
    12061 ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg gtaaattcgg
    12121 ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca tgcctacccg
    12181 cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg
    12241 tatcggccag ggttactgga cagcgacccc aatccagatg agtaaggcac tgatgatcct
    12301 gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg aagacggcaa
    12361 acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc gatattcatt ccggttactg
    12421 ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaatggta cggcgcataa
    12481 atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc aggtcttcgg
    12541 tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac gtgaccacaa
    12601 actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca tgattctgga
    12661 gaacggcggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg accacattat
    12721 gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg cagcggagga
    12781 ccattaatca tgacggctaa tccgaataaa aaaacattct gggataaagt ccatctcgat
    12841 cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt tatctggagc
    12901 gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc gatgggtctg
    12961 gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg ggccccctat
    13021 ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggagc catctctaaa
    13081 ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga aattgccaaa
    13141 atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg
    13201 aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt ggctgcacag
    13261 cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact gttcctctct
    13321 ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt
    13381 ctatggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct cctagacccg
    13441 gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc tattggctcc
    13501 ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga atttctcccc
    13561 gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt agtgggcatt
    13621 ctgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat agccgccaga
    13681 gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa tgctgatatt attcgtttat
    13741 gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt tccgctccca
    13801 ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg
    13861 tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa
    13921 gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg
    13981 tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccca tagttgaaat
    14041 tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg attaccagcg
    14101 cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg cgggactggc
    14161 ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag ctttcgatcc
    14221 gacaaagctg acggcggccc atccaacgct tccgatcccc agctacgcca gaatcactaa
    14281 cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggccctt acggcaacga
    14341 ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa
    14401 agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc ctggtatggc
    14461 ttgtaccaca gtcgccaaac agacttatgc cctgcctgca cctcccgatt taagcggtgg
    14521 cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca gtaattcgac
    14581 gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt agcggtttcc tcggcgctca
    14641 aacgacctta gcgcctggtg tactggaagg cagcgaaccg acgcctgcgc cacagcccgt
    14701 tgttacagct tcgtcgacaa cgcctgcaac ctcgcctgca atggtgacac cgcaagccgc
    14761 ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgtaagcg atcaggctcg
    14821 tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc gcgtaactca
    14881 aaatggcgcg gtctggcgga tccagcttgg cccattcgcc agcaaagccg aagccagtac
    14941 cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacta ccgcgcagta
    15001 gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga
    15061 aagtcagatg cctgccggta gtgcatttgc tatagtaggg cactttttta attccatcac
    15121 ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc
    15181 gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat
    15241 caaaactatg atcccgggtg taccgcagat cgatgcggag tcctacatcc tgattgacta
    15301 taactccggc aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct
    15361 gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga
    15421 aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg
    15481 ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg
    15541 tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg ccgctggtag
    15601 ccaggacgct tttgttggct tgatgaacag ctacgttaac gcactgggcc tgaaaaatac
    15661 ccacttccag acggtacatg gtctggatgc tgatggtcag tacagctccg cgcgagatat
    15721 ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat gaatactcga tctataaaga
    15781 aaaagaattt acgtttaacg gtattcgcca gctgaaccgt aacggcctgt tatgggataa
    15841 cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt
    15901 tgcttctgcg actgaaggcc agatgcgctt gatttctgcg gtgatgggtg gacgtacttt
    15961 taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac
    16021 cgttaaccca ctgaaagtag gtaaagagtt cgcctcagaa ccggtttggt ttagtgattc
    16081 tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccgc gtggtcgcat
    16141 gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa
    16201 gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg agcaacgccc
    16261 gctggttgtg ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat
    16321 taaattaatg ttccatcact ggtttggtta aaaattgaac acttgaaagt gtaatttccg
    16381 tccccatata ctaagcatca gtaaaaaaac tcccaccttc tggcgggagt tgctatttaa
    16441 ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact
    16501 ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa
    16561 gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc
    16621 aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg
    16681 tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta
    16741 cccggctcct ggtcgggtaa ctctcctctc ctcgctgtga tatacttttc ccccactttt
    16801 actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc gccagctcgg
    16861 tcttcagcct tacgagccaa tctcccgggc tatgcatgaa ttcaccgata cccgcgataa
    16921 tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc
    16981 aggaaaagcg gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg
    17041 tgggcaggtg acttatcacg ggccgggaca acaggtgatg tatgtgttgc ttaacctgaa
    17101 acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac
    17161 cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg
    17221 ggaaaagaaa atttgctcac tgggtttacg aattcgacgc ggttgttcat tccacggtct
    17281 ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc
    17341 cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc
    17401 tccacgttta ctggaaaata ttttagcgct actaaacaat ccggacttcg aatatattac
    17461 cgcttaattc catacatcaa tggcccaatt tacactgggt cattactcat aatacctgct
    17521 aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga aatgcggtgc
    17581 cacgccttgc tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga
    17641 gcaaaagcgt tccgttccaa ttagacataa gattattgta attttttaaa tattattttt
    17701 ccatgaataa aattggagta agtgcgtgga tagtaataat caaattgaac cctgtttaag
    17761 cagaaagtcg tcagaaggta aaccacaaat atttacaacc ttacgaaata ttgatcttaa
    17821 ccttctgact atttttgaag ctgtatatgt acataaaggg atcgttaatg cagcgaaagt
    17881 gcttaatctg accccctcgg caatcagtca gtctattcag aaactgcgcg ttatattccc
    17941 tgacccattg tttattcgca aaggccaggg tgtcactcct accgcatttg cgatgcatct
    18001 acatgagtat atcagtcagg gccttgagtc cattcttggc gcgctggata tcgaaggaag
    18061 ctatgataag caacgaacga taactattgc gaccactccc tcggtcggag ccctggtcct
    18121 tcctgtcatc tatcgggcga ttaaaactca ctatccgcag cttttactgc gcaacccgcc
    18181 catcagcgac gcggaaaacc aactcagtca gtttcaaacc gatctcatca tcgataacat
    18241 gttttgcacc aatcgtacgg tgcaacatca tgttctgttc accgacaata tggtgttaat
    18301 ttgccgcaag ggaaatccac tactctcttt agaagatgac agagagacta tcgacaacgc
    18361 tgcgcatgta cttctgttac cggaagggca aaatttcagc ggtctgcggc agagagttca
    18421 agagatgttt ccggaccggc aaatcaattt caccagctac aacattttga caatcgctgc
    18481 actggttgcc aacagtgaca tgttagcgat tattcccagc cgtttttata acctgtttag
    18541 tcgttgctgg ccgctggaaa aactgccttt tccgtcctta aatgaggagc aaatagattt
    18601 ctctatccac tacaacaaat tcagcctgcg tgatcccatc ctgcacggag taatagatgt
    18661 tatccgtaat gcattttgaa aaagtattgt tacagccaga tcacagtgca gaacgataaa
    18721 atggcacaat ccaacaacaa ttttacattt tcgcgaccgc tttggctgct ttcaggtccg
    18781 tttcaatgat atactgccag tcgttaattc aaaaatagtt gataattaca acaatctatt
    18841 gaattgaaac gctttccttc gtaattcgca actggaacac gcacgctatg agtaaaccca
    18901 ttgtgatgga acgcggtgtt aaataccgcg atgccgataa gatggccctt atcccggtta
    18961 aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc
    19021 ttccagcgga ctctacacgt atccagggca tcaaagccgc aatgcgcaaa aatggcctgc
    19081 attctgtctg cgaggaagcc tcctgcccta acctggcgga atgcttcaac cacggcacag
    19141 caacgtttat gatcctcggc gctatttgta cccgccgttg tccgttctgt gatgttgccc
    19201 acggtcgccc ggtagctcct gatgccaatg aaccagtgaa actggcgcag accattgccg
    19261 atatggcgct gcgttatgtg gtcatcacct ccgttgaccg tgatgacctg cgcgatggcg
    19321 gtgcccaaca ctttgcggat tgtattactg ccattcggga aaaaagcccg caaatcaaaa
    19381 ttgaaactct ggtgccggat ttccgcggtc gaatggatcg tgctctggat attctgactg
    19441 caacgccccc agatgtgttc aaccataacc tggaaaacgt accgcgtatt taccggcagg
    19501 tacggcctgg cgcagattac aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc
    19561 atccggaaat cccgaccaag tctggtctga tggtgggtct gggtgaaacc aatgaagaaa
    19621 ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat
    19681 atttgcagcc aagccgccat cacctgccgg ttcaacgtta cgttagcccg gatgagttcg
    19741 acgaaatgaa agccgaagcg ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg
    19801 tccgctcttc ttaccacgcc gatttgcagg cgaaagggat ggaagttaag taatcctgta
    19861 ataattactt acatctgtct gataaaaaac ccgctttttg aagcgggttt tttgtatcga
    19921 acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga gcttttcagc
    19981 ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca tcgccttctt
    20041 gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct tagtcccaaa
    20101 cagcagaacg accagcgccg caactaccag cagtttggta atactaatct cacccataga
    20161 taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccagaga atattaacgg
    20221 tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg ccgagtaaaa
    20281 aaacatcata ataattgcgg cggcgcaaag cgacggttgt ttaagatggg cagttgcgcg
    20341 cgcacctgac gcacacgttc gggcgtcact tccgccataa tgagtgcagg catttctgac
    20401 gctgccgcaa tggtgacgcc aaagggatct ataatccggc tttgcccgat atttttgttc
    20461 ccgcactccc ccgccgccac catataacag gtggtatcca gcgcacgagc ggcaagcaac
    20521 gttgaccagt gatgctcttt gagcggccca cgaacccagg cggcaggaag taccaggatt
    20581 tcagctccct gtaaagcctg tgccagcgcc agctctggaa agcgtaagtc ataacaggtc
    20641 atcagaccga ccttcatccc ttccacctcc agtaacggag ctatttcatt accagcatca
    20701 acatggcgtg attcctgaat ggcaaatgca tcatagagat gcagtttggc ataacgggcg
    20761 acgatgtttc ctgcctgaag tgccaccagc atattccatg cgcgccccgg cgttgaagga
    20821 agatgaatcg tcagaattgt cgtcatcatg ttacgtttac tttctcgccg taaacgtccg
    20881 aggaattcgc cttccagcag ctgtgctgat ttaaccgata gatctgcatc atgatcatcg
    20941 cgcgccagca atgcttccgg caggacaaac agcgatacgt cgttttccgc cgcctgcgcc
    21001 atcaacgagg cacaaatctc agcgttcttt tcccacacag atgtaacagc aaactgtcct
    21061 gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa ttcgccaacg acgattattc
    21121 ggcgttacac ttatcactca tacaaatcaa atagcaggat tttgcagtgt tacaacttct
    21181 tttagcagtt tttattggcg gtggtacggg aagcgtagcg agatggctgt taagtatgcg
    21241 atttaacccg ctgcatcagg cgattccgtt ggggacgctg gcagcaaacc tgattggggc
    21301 attcatcata ggaatgggat tcgcctggtt cagcaggatg acgaacattg atccagtgtg
    21361 gaaagtatta atcaccaccg gattttgtgg cggtctaaca accttctcaa cattttcggc
    21421 agaagtggtg tttttgttac aagagggccg ctttggctgg gcattactga acgttttcgt
    21481 caaccttctg gggtcttttg ccatgaccgc actggcattc tggctgtttt cggcctcaac
    21541 cgcacactaa agaccaaaaa aaacccgctg attaagcggg ttttgaattc ttgctgacgt
    21601 atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag tgatttcgaa
    21661 ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg cagagaagtg
    21721 tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt
    21781 aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat gaaaaataga
    21841 cacaaatgct gtgtcggtta tcagtacacc aattgtggta cgctttgtcc agtgcaactt
    21901 tactaaaaag tgataaaagt cgcgttattt ttgatgttgc ttattttagt tattgatttt
    21961 aaagaagtta ctaaaacttc atttgcctca aaactgaaag cgcatccagg caaagtacac
    22021 attgccattg ttgtaggtac ccggaatgta ggtcatctga aaagtcactg ggccataacc
    22081 cacggaggcc aatggcagta gaaccgggag agggatgtaa ttccagttat cgcgtgccgt
    22141 tacgccagcg gtgaatccca gacctaaatg aaaattttca tccgccagcg gtcgccaggt
    22201 actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct taaatgccat
    22261 ggcatacagg ccatgccagt ttcctttttc atcccaacgc gacaggccaa aaccgccacc
    22321 ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca
    22381 ggtgatggca ggaatatata aatcataatg ttcaggctgt tgccaggttt gtgcaatatt
    22441 ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa actttaccaa cgctgattaa
    22501 ctgaataaaa acaaaggaaa agatagcgac atatttactc acgttcattt gtgaccataa
    22561 aacatttatc aaaaatctac tactagcata gcaaagctac aattaacata accttaatag
    22621 acccaacata aagaataatc tgaatagctg ctctatttat ttccgcttgt tcaagttaaa
    22681 tatttccgcg catcgattaa agatgatgcg tcgttttctg cgatgggaat agtcaaaaaa
    22741 gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg aaaaattatt ttattttttt
    22801 aacgagatac aacaatcatc ttaacgaagt atataatata tatcgtcaac ccatgctcat
    22861 attagattat tttttgagcg cacagccgca gctaacacca ttgcaattaa caaatttgca
    22921 tcaatccacc atcaatttac acacattatt atgtgataat tgccaaccgc taaatatgcg
    22981 ttttgttatc tatgtataaa aacagcaact tcaatgtctt aatggcagtt tttcttgatt
    23041 ttaatcagca ttcatcgcca atttattggg catatttttt ccttaagctt taggaagttt
    23101 ttattattta ctttgggcct ggagacagga aaaattatgc tgacattcat tgaactcctt
    23161 attggggttg tggttattgt gggtgtagct cgctacatca ttaaagggta ttccgccact
    23221 ggtgtgttat ttgtcggtgg cctgttattg ctgattatca gtgccattat ggggcacaaa
    23281 gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca cggatatcgt tgaatacgtt
    23341 aaaatattac taatgagccg cggcggcgac ctcggcatga tgattatgat gctgtgtggc
    23401 tttgccgctt acatgaccca tatcggcgcg aatgatatgg tggtcaagct ggcgtcaaaa
    23461 ccattgcagt atattaactc cccttacctg ctgatgattg ccgcctattt tgttgcttgt
    23521 ctgatgtcac tggccgtctc ttccgcaacc ggtctgggtg ttttgctgat ggcaaccctg
    23581 tttccggtga tggtaaacgt tggtatcagt cgtggcgcag cagctgccat ttgtgcctcc
    23641 ccggcggcga ttattctcgc accgacttca ggggatgtgg tgctggcggc gcaggcttcc
    23701 gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc ctatctcaat tgctgcaatt
    23761 atcggcatgg cgatcgccca cttcttctgg caacgttatc tggataaaaa agagcacatc
    23821 tctcatgaaa tgttagatgt cagtgaaatc accaccactg cccccgcgtt ttatgccatt
    23881 ttgccgttca cgccgatcat cggtgtactg atttttgacg gtaaatgggg tccgcaatta
    23941 cacatcatca ctattctggt gatttgtatg ctgattgcct ccattctgga gttcctccgc
    24001 agctttaata cccagaaagt tttctctggt ctggaagtgg cttatcgcgg gatggcagat
    24061 gcgtttgcta acgtggtgat gctgctggtt gccgctgggg tattcgctca ggggcttagc
    24121 accatcggct ttattcaaag tctgatttct atcgctacct cgtttggttc ggcgagtatc
    24181 atcctgatgc tggtattggt gattctgacc atgctggcgg cagtcacgac cggttcaggc
    24241 aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac tggcgcactc ttccggcatt
    24301 aacccggcgt atttgactat cccgatgctg caggcgtcaa acctgggtcg taccctatca
    24361 cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga aaatctcacc atttgaagtc
    24421 gtaaaacgca cctcggtgcc ggtgcttgtt gggctagtga ttgttatcgt tgctacagag
    24481 ctgatggtgc caggaacggc agcagcggtc acaggcaagt aaatagtaat gccggaggca
    24541 agtttcctcc ggctttatca tgtcacccac tgtggtatat gcgttgtggt ctgccaactt
    24601 tgccgtgaac aatttcagca ataatcagat ggcggctggc gcaatattca agataacgcc
    24661 tggcagtggt gcggctgatg gtcagtgcct gcgccaccgt ttctgccgta tgttgcacac
    24721 caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa ggggtcaatg ccggtcggca
    24781 gttcgtcttt gggttcaccg cgagcatagg cattaaacat ctcatcaatt tgcttctggc
    24841 tggcgctatc aatactttcc agcatatgtt tacactggcg gaaacgggtt ag
//
LOCUS       DAJTYI010000048        24840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYI010000048 DAJTYI010000000
VERSION     DAJTYI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..2167
                     /gene="adiA"
                     /locus_tag="OO564_001924"
     CDS             <1..2167
                     /gene="adiA"
                     /locus_tag="OO564_001924"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6653066.1"
                     /translation="VIKSTSFDDGFAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGK
                     LHERQQNVPVFLLGDREKALAAMDRDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQ
                     QLLPPLFSALMKYSDIHEYSWAAPGHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIER
                     TSLGSLLDHTGAFGESEKYAARVFGADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDR
                     NCHKSIEQGLMLTGAKPVYMVPSRNRYGIIGPIYPQEMQPETLQKKISESPLTKDKAG
                     QKPSYCVVTNCTYDGVCYNAKEAQDLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRG
                     EPGDHNGPTVFATHSIHKLLNALSQASYIHVREGRGAINFSRFNQAYMMHATTSPLYA
                     ICASNDVAVSMMDGNSGLSLTQEVINEAVDFRQAMARLYKEFTADGSWFFKPWNKEVV
                     TDPQTGKTYDFADAPTKLLTTVQDCWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPG
                     MGEDGELEETGVPAALVTAWLGRHGIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCS
                     FKRHYDANTPLAQVMPELVEQYPDTYANMGIHDLGDTMFAWLKENNPGARLNEAYSGL
                     PVAEITPREAYNAIVDNNVELVSIENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSP
                     QVSYLRSLQSWDHHFPGFEHETEGTEIIDGIYHVMCVKA"
     gene            2492..3253
                     /gene="adiY"
                     /locus_tag="OO564_001925"
     CDS             2492..3253
                     /gene="adiY"
                     /locus_tag="OO564_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6653067.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3390..4727
                     /gene="adiC"
                     /locus_tag="OO564_001926"
     CDS             3390..4727
                     /gene="adiC"
                     /locus_tag="OO564_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6653068.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARIALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4831..6474
                     /gene="eptA"
                     /locus_tag="OO564_001927"
     CDS             4831..6474
                     /gene="eptA"
                     /locus_tag="OO564_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6653069.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6471..7139
                     /gene="basR"
                     /locus_tag="OO564_001928"
     CDS             6471..7139
                     /gene="basR"
                     /locus_tag="OO564_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6653070.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7140..8240
                     /gene="pmrB"
                     /locus_tag="OO564_001929"
     CDS             7140..8240
                     /gene="pmrB"
                     /locus_tag="OO564_001929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6653071.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8216..8305)
                     /gene="pmrR"
                     /locus_tag="OO564_001930"
     CDS             complement(8216..8305)
                     /gene="pmrR"
                     /locus_tag="OO564_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6653072.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8417..9919)
                     /gene="proP"
                     /locus_tag="OO564_001931"
     CDS             complement(8417..9919)
                     /gene="proP"
                     /locus_tag="OO564_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6653073.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10183..11061)
                     /locus_tag="OO564_001932"
     CDS             complement(10183..11061)
                     /locus_tag="OO564_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6653074.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11058..13286)
                     /gene="crfC"
                     /locus_tag="OO564_001933"
     CDS             complement(11058..13286)
                     /gene="crfC"
                     /locus_tag="OO564_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6653075.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13688..14023
                     /locus_tag="OO564_001934"
     CDS             13688..14023
                     /locus_tag="OO564_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6653076.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14181..14624
                     /gene="yjdN"
                     /locus_tag="OO564_001935"
     CDS             14181..14624
                     /gene="yjdN"
                     /locus_tag="OO564_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6653077.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14757..15545
                     /gene="phnC"
                     /locus_tag="OO564_001936"
     CDS             14757..15545
                     /gene="phnC"
                     /locus_tag="OO564_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6653078.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15570..16586
                     /gene="phnD"
                     /locus_tag="OO564_001937"
     CDS             15570..16586
                     /gene="phnD"
                     /locus_tag="OO564_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6653079.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16692..17471
                     /gene="phnE"
                     /locus_tag="OO564_001938"
     CDS             16692..17471
                     /gene="phnE"
                     /locus_tag="OO564_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6653080.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17492..18217
                     /gene="phnF"
                     /locus_tag="OO564_001939"
     CDS             17492..18217
                     /gene="phnF"
                     /locus_tag="OO564_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6653081.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18218..18670
                     /gene="phnG"
                     /locus_tag="OO564_001940"
     CDS             18218..18670
                     /gene="phnG"
                     /locus_tag="OO564_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6653082.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18667..19251
                     /gene="phnH"
                     /locus_tag="OO564_001941"
     CDS             18667..19251
                     /gene="phnH"
                     /locus_tag="OO564_001941"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6653083.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19251..20315
                     /gene="phnI"
                     /locus_tag="OO564_001942"
     CDS             19251..20315
                     /gene="phnI"
                     /locus_tag="OO564_001942"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6653084.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20308..21153
                     /gene="phnJ"
                     /locus_tag="OO564_001943"
     CDS             20308..21153
                     /gene="phnJ"
                     /locus_tag="OO564_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6653085.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21150..21908
                     /gene="phnK"
                     /locus_tag="OO564_001944"
     CDS             21150..21908
                     /gene="phnK"
                     /locus_tag="OO564_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6653086.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADPRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22019..22699
                     /gene="phnL"
                     /locus_tag="OO564_001945"
     CDS             22019..22699
                     /gene="phnL"
                     /locus_tag="OO564_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6653087.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22696..23832
                     /gene="phnM"
                     /locus_tag="OO564_001946"
     CDS             22696..23832
                     /gene="phnM"
                     /locus_tag="OO564_001946"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6653088.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23832..24389
                     /gene="phnN"
                     /locus_tag="OO564_001947"
     CDS             23832..24389
                     /gene="phnN"
                     /locus_tag="OO564_001947"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6653089.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24376..24810
                     /gene="phnO"
                     /locus_tag="OO564_001948"
     CDS             24376..24810
                     /gene="phnO"
                     /locus_tag="OO564_001948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6653090.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
BASE COUNT         5898 a         6975 c         6491 g         5476 t
ORIGIN      
        1 cgtgattaaa tccacctcct ttgatgatgg ttttgccatt ctctcttcaa acgaagccat
       61 tgactgcctg atgttcagct atcaaatgga acatccggac gaacatcaaa acgtcagaca
      121 attgatcggt aagcttcatg agcgccaaca aaacgtgccg gtcttcctgt tgggcgatcg
      181 ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag cttgtcgatg aattcgcctg
      241 gattctggaa gataccgccg actttatcgc cggacgcgcc gttgccgcga tgacccgcta
      301 ccgccagcag ctgttgccgc cactgttcag cgcgctgatg aaatatagtg acatccatga
      361 atattcctgg gcagcgccag gccaccaggg cggcgttggt tttaccaaaa cacccgccgg
      421 acgtttctac catgactact atggtgaaaa tctgttccgc accgacatgg gcatcgaacg
      481 aacttccctc ggttctttgc ttgaccatac tggcgcattt ggcgaaagcg aaaaatatgc
      541 cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc gtcggtactt ccggctctaa
      601 ccgcaccatc atgcaggctt gcatgaccga taacgatgtc gtggtcgttg accgtaactg
      661 ccataaatcc atcgaacaag gtttgatgct gacaggcgcg aaaccggtct atatggtgcc
      721 aagccgcaac cgctacggca ttatcgggcc aatctatccg caggaaatgc aacctgaaac
      781 cttgcaaaag aaaatcagtg aaagcccgct gaccaaagac aaagccgggc aaaaaccgtc
      841 ttactgcgtg gtaaccaact gcacctatga cggcgtgtgt tataacgcta aagaagcgca
      901 ggatctgctg gaaaaaacct ccgatcgtct gcactttgac gaagcctggt acggctatgc
      961 acgtttcaac ccgatctatg ccgatcacta tgccatgcgc ggcgaacctg gcgatcacaa
     1021 cggtcctacc gttttcgcca cccactccat ccacaaactg ctgaatgcgc tgtcacaggc
     1081 ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac ttctcccgct tcaaccaggc
     1141 ctacatgatg catgccacca cctccccgct gtatgccatc tgcgcatcca acgacgtggc
     1201 ggtgtcgatg atggacggca acagcggcct gtcactgaca caggaagtga ttaacgaagc
     1261 ggttgatttc cgtcaggcga tggcgcggct atataaagag ttcaccgctg acggtagctg
     1321 gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca caaaccggca aaacctatga
     1381 ctttgctgac gcaccaacca aactgctgac caccgttcag gactgctggg taatacatcc
     1441 gggcgaaagc tggcacggct tcaaagatat tccggataac tggagtatgc tcgacccaat
     1501 taaagtcagc atccttgctc cgggaatggg tgaagatggt gaactggaag aaaccggtgt
     1561 tccggcggcg ctggtcactg cctggcttgg tcgccacggc attgtgccta cccgcaccac
     1621 tgacttccaa attatgttcc tgttctctat gggcgtaacc cgtgggaaat gggggactct
     1681 ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc aacacaccgc tggcgcaggt
     1741 gatgccggaa cttgttgaac aatatcctga cacttacgcg aacatgggga ttcacgatct
     1801 gggtgacacc atgtttgcct ggctgaaaga aaacaaccct ggcgcacggt tgaacgaagc
     1861 ctattccggc ctgccggtgg cggaaatcac cccgcgtgaa gcgtacaacg cgattgtcga
     1921 caacaatgtc gaactggtat ccattgaaaa tctgccagga cgcatcgcgg caaactcagt
     1981 tatcccgtat ccgccaggaa tcccgatgct gctgtctggt gaaaacttcg gcgataaaaa
     2041 cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg gaccaccatt tccctggatt
     2101 tgaacacgaa actgaaggga ctgaaattat tgacggtatt taccacgtta tgtgcgtgaa
     2161 agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat aacattacgc cgcctggcct
     2221 taggcctttt gagtatggca acgttttcat aaaaattgct gcaaacaaaa atgtcatact
     2281 ttttgcgcgg ccccaccccg cgcttttttt tgcctgttat ttatcctgta aaaaaatatg
     2341 tacatgagaa attactataa aaatttgtaa tattagtaaa actcgttatt tttatgcatg
     2401 tttatattca tcatataatt atataaccat ttcccggtat cgctttgctt tagcgagaac
     2461 tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
     2521 tgttttattg actgaaaaag atgtccggat aagggtgaat gggaaagaac ctattagcct
     2581 taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
     2641 taacaacact ttggttgctc atattagtca cgacatcatc aaagactacc tccggtttct
     2701 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca tactcaccct
     2761 gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga
     2821 aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct
     2881 cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag
     2941 cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag
     3001 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagttg aaaagcgaaa acaccagtta
     3061 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
     3121 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
     3181 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
     3241 cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
     3301 cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
     3361 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
     3421 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     3481 tacctgcaaa cctggcctcc actggcggga ttgccattta tggatggttg gtgacgatta
     3541 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg
     3601 gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     3661 tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat
     3721 atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg
     3781 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgcgtgc
     3841 aggccgttgc cactgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     3901 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcaccttcg
     3961 gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     4021 ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt ccctatcgcc accattggtg
     4081 gggtattgat tgccgccgtt tgctatgtgc tctccaccac cgcgattatg gggatgatcc
     4141 ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg atagcgttgg
     4201 gtgacaccgc tggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     4261 gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     4321 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
     4381 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
     4441 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
     4501 cgttactgct gctcggccac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
     4561 ctaccattgc cttcctctac tgcatttggg ccgtggtggg gtccggggcg aaagaggtta
     4621 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
     4681 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
     4741 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     4801 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
     4861 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
     4921 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
     4981 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
     5041 ttcttatggc ttaatcgaac actggcctgc ctgtttattc tggttggcgc ggctgcacaa
     5101 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
     5161 accactccag cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
     5221 agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa tcaaacctgc cacctcgcgt
     5281 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     5341 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     5401 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     5461 ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc gttaatgcag
     5521 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     5581 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     5641 tatttcccta ataccgcatc ttgcggtacg gcaacggcag tttcagtacc gtgcatgttc
     5701 tcggatatgc cgcgtgagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg
     5761 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     5821 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tgatcagtgc
     5881 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     5941 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     6001 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     6061 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     6121 attgttgata aagcgattaa tctgcttaaa gaacatcagg ataaatttac caccagcctg
     6181 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     6241 ccttatgcca tcgccccgga tagccaaaaa caggtgccaa tgctgctgtg gctgtcggag
     6301 gattatcaaa agcggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     6361 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     6421 tattaccagg ccgcggatga tattctgcaa acttgcagga gagtgagtaa atgaaaattc
     6481 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     6541 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggacggtc
     6601 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     6661 cccgtatccg gcagaaaaaa tacaccctgc cggtactgat cctcaccgct cgcgatacgc
     6721 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     6781 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     6841 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     6901 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     6961 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     7021 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     7081 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     7141 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     7201 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     7261 ccgagcagat tcaactgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     7321 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     7381 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     7441 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     7501 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     7561 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     7621 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     7681 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     7741 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     7801 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     7861 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     7921 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     7981 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     8041 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     8101 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     8161 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     8221 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     8281 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     8341 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     8401 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     8461 tcgcctgcaa atcggcaatc tcgtggtcaa tattatcgat tttctgctcg atattatcgt
     8521 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     8581 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     8641 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     8701 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     8761 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccataaagc
     8821 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     8881 taatcagaat aaacgccggg atcgccaaca caaacagtgc aacactacca agtagcacaa
     8941 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     9001 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     9061 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     9121 gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt
     9181 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga
     9241 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     9301 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     9361 aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc
     9421 agctgcccat aaagccacgt ttacggtcgg gggagtattc cgcaacaaat atcgacgccc
     9481 cggtatattc accaccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     9541 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     9601 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     9661 caaagaagag tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     9721 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     9781 caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa
     9841 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta
     9901 cttttttcct tttcagcata gctttcctcg cagagttgac ccatttccgc aaacgccaga
     9961 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
    10021 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
    10081 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
    10141 acacatggat ttcgtttagg actcattgat gtaactgatt atttacgcca aatttacaga
    10201 gaagatttcc tcgaccattg cccccaccaa acgctgcgct gaacagagtc gtggcatccc
    10261 cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt
    10321 ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga
    10381 agaggcaaac cactgatggt aatagtgacg cgtggtcggt gccgcattga tactctctgc
    10441 cagatgttgt gtcgctttct cgcgcaaatc acccagcaac aacaccaccg cttgcgggct
    10501 tcccagacgg caggcatcgc caaatgcacc ccagcgcaac tcctccagaa cgataaaaca
    10561 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
    10621 gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg
    10681 acaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct ccgggaaagc
    10741 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
    10801 cgccagatga ccgggatcca gcatcgacgc cagtttttcc accattgtca gtcgcagact
    10861 aagcggcgca tttaacatgc cgctgttcgt ccatgtctga agctgcacac gcgaaagaag
    10921 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgaggatggc gggcatcatc
    10981 gccctgcgcc agatccacta aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc
    11041 gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
    11101 aacagctgag cgtcggtgta aatccagctg tgagtcatca cagtctgctg caattgctgc
    11161 tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
    11221 gtctgtaatt ctgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
    11281 acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata gagatcgact
    11341 tctgcgctta atgctcgttg caacacactg taatcaaccg tttctgtcgg ggatttctca
    11401 tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct
    11461 aaacgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg
    11521 cgagtgttga agtggggaac tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac
    11581 cctgactcgc tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac
    11641 acccggcaaa gggtggattc cagctcggca aaacgcagcg taaagtcgcg actgatggtg
    11701 ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa
    11761 tcatcctgaa acaaggcggc taactgcgca ttcaccgcat cttgctgttg acgcagaaag
    11821 tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc
    11881 tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg ccgcaattgt
    11941 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg
    12001 taattcaaca gtttatgcgc agcagatcgc agagcataca atgaggcgtt agcgtaagcg
    12061 gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
    12121 gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
    12181 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg ctggtaactt accgttgttg
    12241 gctaactcat agcgcgcccg attcgccagg tagccccaca tcgacgacac cgggaatatc
    12301 tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg
    12361 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc
    12421 gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc
    12481 gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt
    12541 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
    12601 ggataacttt ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
    12661 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcactaaa
    12721 tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa
    12781 gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
    12841 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaattgttg aattaaacaa
    12901 tcgattggcg cgacatgtga aaaatgcagt accggttcct tttgcccggg cgtatggcga
    12961 ataagcgtcg gcagcgcagt cattgggcga ttacgattag gcagaacctc cgtaccaaca
    13021 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaattgc cagcaccatt
    13081 tccagtcggg aaattttacg caactcatta ttcagcatcg cgtgacgttc ggcgatatta
    13141 ggctgactcc agggtaaagc cagttgtggc gcgtcgtctc cgggtacaga gagaggcatt
    13201 ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt
    13261 aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat tttgttactt
    13321 ttatttagcc agattgtttt tgagttctgt tttcggcttt tataattact gcaagaaata
    13381 attttatatt tagtgtgttg ttttttatca gaataaataa cgtcttctga tgcgtttaaa
    13441 acgtcagaaa gataaaaata tcatgtaaat aaaaaaaaga acaagtagag cattaacatt
    13501 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
    13561 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
    13621 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
    13681 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
    13741 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
    13801 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
    13861 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
    13921 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
    13981 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
    14041 agccgaataa ggcgctcgcg ccgcatccgg caacgatgcc gactgcctga tgcgacgctt
    14101 gcgcgtctta tcaggcagcc tctcatctgt ataaatttcg aactacactt aactggcttc
    14161 tcttaactga ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt
    14221 ccgacgcgat tgcctattat caacgtacgt tgggcgcgga actgctctat aaaatcagct
    14281 tcggcgaaat gccgaaatca gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat
    14341 ttcccgatac cgccatcgct catgccaacg tgcgcattgc cggaagcgac atcatgatga
    14401 gcgatgctat accgtcagga aaagccagct actccggctt tacgctggtg ctcgattcgc
    14461 aacaggtcga agaaggaaaa cgctggtttg ataaccttgc cgctaacgga aaaatcgaaa
    14521 tggcctggca ggaaactttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg
    14581 taccgtggat gattaatgtc gtcaaacaac aaccaacgca ataacccgcc gggaggcccg
    14641 ccctcccgca ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa
    14701 ccaaatcgtc acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc
    14761 aaacgattat ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcaa gcgctgcatg
    14821 cggttgatct gaacattcat cacagtgaaa tggtggctct gcttgggccg tcgggttccg
    14881 gcaaatccac ccttttacgt catttaagcg gtttgattac cggcgataaa tccgccggta
    14941 gccatatcga gctactgggc cgcactgtcc agcgcgaagg ccgcctggcc cgcgatatcc
    15001 gcaaaagccg cgccaacacc ggctacatct ttcaacaatt caacctggtg aaccgcctga
    15061 gcgtactgga gaacgtgctg attggcgcgc tcggcagcac gccgttctgg cgcacctgtt
    15121 ttagctggtt caccggcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca
    15181 tggtgcattt tgcccatcag cgcgtttcca ccctctccgg cggccagcag caacgtgtgg
    15241 cgattgcccg tgcgctgatg cagcaggcga aagtgattct ggccgatgaa cccatcgcct
    15301 cgctggaccc ggaatcagcg cgcatcgtga tggacaccct gcgcgacatc aaccagaacg
    15361 acggcatcac cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac
    15421 gcatcgtcgc cctgcgtcag gggcacgtct tctacgacgg cagcagccaa ctgtttgata
    15481 acgaacgttt tgaccatctc taccgcagca ttaaccgcat cgaagagaac gcgaaagctg
    15541 cctgatatcc ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct
    15601 tcaccagcat gttcagcctc agcaccctgt taaccccggc acacgccgaa gagcaggaaa
    15661 aggcgctgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga
    15721 cgccgttttt acaggatatg gagaagaaac tgggcgtgaa ggtcaacgcc ttctttgccc
    15781 cggactacgc gggcattatc caggggatgc gcttcaataa agtggatatt gcctggtacg
    15841 gcaacctgtc ggcgatggaa gcggtggatc gcgccaatgg ccaggtcttc gcccagacgg
    15901 tcgcggcgga tggatcgtcg ggttactgga gcgtgttgat cgtcaacaaa gacagcccga
    15961 tcaacaacct gaacgatctg ctggcgaagc ggaaagatct caccttcggc aatggcgatc
    16021 ctaactccac ctctggcttc ctcgtccccg gttactacgt cttcgctaaa aacaatatct
    16081 ccgccagcga cttcaagcgc accgtcaacg ccgggcatga aaccaacgcg ctggccgtcg
    16141 ccaacaagca ggtggatgtt gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct
    16201 ccgcgccgga gaagcttaaa gagctgaagg tgatctggaa atcgccgctg atcctaggcg
    16261 atccgatcgt ctggcgcaag aatctgtccg aaactaccaa agacaagatc tacgacttct
    16321 ttatgaacta cggaaaaacg ccggaagaga aagcggtgct ggaacgccta ggctgggcgc
    16381 cgttccgcgc ttccagcgac ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag
    16441 agatacaaag cgtgaaaggc aataaagggc tgaatgagca ggacaagctg gcgaaaacca
    16501 ccgagattca ggcgcagctg gatgacctgg accgcctgaa caacgcgtta agcgcgatga
    16561 gttcggtgag taaagcggtg cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat
    16621 ccgacaatgt gcaggcgttg atgccggatg cggtgcaagc accttatccg gcctacagac
    16681 cggagccaaa catgcaaacc atcaccatcg ccccacccaa gcgtagctgg ttctcgcttc
    16741 tgagctgggc cgttgttctc gccgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc
    16801 cgcttacgct gattaaagac ggcggcaaca tggcaacctt cgctgccgac ttcttcccgc
    16861 ccgatttcag ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg
    16921 tctggggcac cgcgctggca gtggttctct ccatcccctt cggtctgatg agcgccgaaa
    16981 acttggtgcc gtggtgggtt taccagcccg ttcgccgcct gatggacgcc tgccgcgcca
    17041 ttaacgaaat ggtcttcgcc atgctgttcg tggtcgccgt cggcctcggc ccgttcgctg
    17101 gcgtgctggc gctgtttatc cacaccaccg gcgtgctctc caagctgctt tccgaagcgg
    17161 aggaagcgat cgaacccggc ccggtggaag gcattcgcgc caccggcgcc aacaaactcg
    17221 aagagatcct ttacggcgtg ctgccgcagg tgatgccgct gctgatctcc tactccctct
    17281 atcgcttcga atccaacgtc cgctcggcga ccgtcgtcgg catggtcggc gcgggcggga
    17341 tcggcgtcac cctgtgggaa gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga
    17401 tggtgcttat catcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc
    17461 actttatctg ataagcgagg cattgatatc tatgcacttg tctacacatc cgaccagcta
    17521 cccaacacgc tatcaggaga tagccgcaaa acttgagcag gagcttcgtc aacactaccg
    17581 ctgcggcgac tatcttcccg ccgagcagca actggcggcg cgctttgagg tgaaccgcca
    17641 caccctgcgc cgcgccatcg accaactggt ggaaaaaggc tgggtacagc gccgtcaggg
    17701 cgtcggcgtg ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt
    17761 tagccagaac ctgctggacc agggcagcca tcccaccagc gaaaaactgc tgtcggtatt
    17821 gcgccccgcg tccggtcacg ttgctgacgc gctggggatt accgagggcg agaacgttat
    17881 ccatttgcgc accctgcgcc gggtcaacgg cgtcgcgctc tgtttaatcg accactactt
    17941 tgcggacctc accctttggc cgacgctgca acgcttcgac agcggttcgc tgcacgattt
    18001 tctgcgcgag caaaccggaa ttgcgctgcg ccgcagccag acgcggatca gcgcccgccg
    18061 cgcccaggcc aaagagtgcc agcgccttga aatcccgaat atgtcgccgc tgctgtgcgt
    18121 gcgcaccctt aaccaccgtg acggtgaaag cagcccggcg gagtactccg tcagcctgac
    18181 gcgcgccgac atgattgaat tcactatgga gcactgaatg cacgcagata ccgcgacccg
    18241 ccagcactgg atgtccgtac tggcgcacag ccaaccggct gaactggcag cccgcctgaa
    18301 cgcgctgaac atcaccgccg actatgaagt gatccgcacc gccgaaaccg gcctggtaca
    18361 gattcaggcg cgaatgggcg gcaccggcga acgctttttt gccggcgacg ccacgctgac
    18421 ccgcgccgcc gtacgcctga ctgacggcac cctcggctac agctgggtgc tggggcgtga
    18481 taaacagcac accgaacgct gcgcgctgat tgatgcgctg atgcagcaat ctcgccactt
    18541 tcaaaactta tcagaaaccc ttattgcccc gctggacgct gaccgtatgg cacgcattgc
    18601 cgcacgccag gccgaagtga acgccagccg ggtcgacttc tttacgatga ttcgcggaga
    18661 caacgcatga ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt
    18721 cgtcgcctgt taaaggccat aagcgagccg ggcgtgattg tcgccctgca tcagctcaag
    18781 cgcggctggc agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggctgataac
    18841 gacacgccgg tgtggcttgc cgcgccattg agtaacgata tcgtcaatca gagcctgcgt
    18901 tttcatacca acgcgccgct ggtcagccag ccggaacagg cgaccttcgc ggtgacggat
    18961 gacggaattt ccagcaaaca gctcaacgcc ctttccaccg gcaccgccgt tgcgccggaa
    19021 gcgggcgcga cgctgatttt acaggtcgcc agcctgagcg gcgggcgcat gttgcgtctc
    19081 accggcgcgg gtattgccga agaacgaatg atcgctccgc agctgccgga gtgcattctg
    19141 cacgaactca ccgagcgccc gcacccgttc ccgctcggca tcgacctgat cctgacctgc
    19201 ggcgaacgcc tgctggctat tccgcgaacc actcatgtgg aggtgtgctg atgtacgttg
    19261 ccgtgaaagg gggcgaaaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc
    19321 ggggcgatac cgatttgccc gaactgagcg tcgcccaaat tgaacagcag cttaatctcg
    19381 cggtggatcg cgtgatgacc gaaggcggcg ttgccgaccg cgaactggcg gcgctggcgc
    19441 tgaaacaggc cagcggcgat aacgttgaag caattttcct gctgcgcgcc taccgcacca
    19501 cgctggcgaa actggcggta agcgagccgc tcgacaccac cgagatgcgt ctcgaacggc
    19561 gtatttcggc ggtttataaa gacattcccg gcggccagct gcttggccca acctacgact
    19621 acacccatcg cctgctcgat ttcaccctgc tggcaaacgg tgaagcgccg acgctgacca
    19681 ccgccgacag cgaacaacag ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc
    19741 tggcgaagtt tgaagaagat agcggcgcac agccggatga catcacccgc acgccgccgg
    19801 tttacccctg ctcacgctcc tcccgcctgc aacaattaat gcgcggggac gaaggctatt
    19861 tgctggcgct ggcctactcc acccagcgtg gttacggacg caatcacccg ttcgcgggcg
    19921 agatccgcag tggttacatc gacgtgtcga ttgtgccgga agagctggga tttgcggtaa
    19981 acgtcggcga actgctgatg accgagtgtg aaatggtcaa cggttttatc gacccgccgg
    20041 atgagccgcc gcacttcacg cgcggctacg ggctggtatt cggcatgagc gagcgcaaag
    20101 cgatggcaat ggcgctggtt gaccgtgcgt tgcaagcccc ggaatacggc gagcacgcga
    20161 caggcccggc gcaggatgaa gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag
    20221 gctttgtctc gcacctcaaa ctcccccact acgtcgattt ccaagccgaa ctggagctac
    20281 tcaaacgtct gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac
    20341 ctcgacgagc agaccaaacg catgatccgc cgcgccatct taaaagcggt ggctatcccc
    20401 ggttatcagg tgccgtttgg cgggcgcgag atgccgatgc cctacggctg gggaaccggc
    20461 ggcattcagc tcaccgccag cgtgattggc gaaagcgacg tgttgaaggt gattgaccag
    20521 ggcgcagacg acaccactaa cgccgtgtcg attcgcaact tcttcaagcg cgtgaccggg
    20581 gtgaacacca ctgaacgtac ggacgatgcg acgcttatcc agacgcgcca tcgcatcccc
    20641 gaaacgccgc tgaccgaaga tcagatagtt attttccagg tgccgatccc cgaaccgctg
    20701 cgctttatcg agccgcgcga aacggaaacc cgcactatgc acgcgctgga ggagtacggc
    20761 gtgatgcagg tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc caccacctat
    20821 gcttatccgg tgaaggtgaa cgggcgctac gtgatggacc cgtcgccgat cccgaaattc
    20881 gataacccga aaatggacat gatgcccgcc ctgcagctgt tcggcgcagg gcgcgagaag
    20941 cgcatctatg cggtgccgcc gtttacccgc gtggaaagtc tcgatttcga cgatcacccg
    21001 ttcaccgttc agcagtggga tgagccatgc gccatctgcg gatcgaccca cagctatctc
    21061 gacgaagtgg tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat
    21121 tgccgccaac agagcgaggc aaaaaaccaa tgaatcaacc gttactttcg gtcaataacc
    21181 tgacccacct ttacgcgccg ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg
    21241 gggaagtgct gggcatcgtc ggggaatccg gctccgggaa gaccacgctg ctgaaatcaa
    21301 tctccgcgcg cctgacgccg cagcaggggg agattcgcta cgagaatcgt tcgttgtatg
    21361 cgatgagcga ggccgacccc cgtcgcctgc tgcgcaccga atggggcgtg gtgcatcagc
    21421 atccactcga cggcctgcgt cgccaggtct cggcaggcgg caatatcggc gagcggctga
    21481 tggcgaccgg ggcccgtcat tacggcgaca tccgcgccac cgcgcagaag tggctggaag
    21541 aggtggagat tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggcatgc
    21601 agcaacgttt gcagattgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggacg
    21661 agccaaccgg tgggctggat gtgtcggtgc aggcacgcct gctcgatcta ctgcgcggcc
    21721 tggtggtgga gctgaacctg gcggtggtga ttgtcaccca tgatttaggc gtcgcccgcc
    21781 tgctagcgga ccgtttgctg gtgatgaagc aggggcaagt agtggagagt gggttaaccg
    21841 accgcgtgct cgacgacccg caccatccgt atacccagct gctggtgtca tcggttttgc
    21901 agaattgagc cggtgccgga tgcggcgtaa acgccttatc tggcctacaa atgcgctccc
    21961 cgtaggtcgg ataagacgca tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat
    22021 gattaacgta caaaacgtca gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct
    22081 gcccgtcctc aaccgcgcct cgctcaccgt caacgcgggc gaatgcgtgg tgctccacgg
    22141 ccactccggc agcggcaaat caaccctgct gcgctcgctg tacgccaact atctgcccga
    22201 tgaagggcaa atccagatca aacacggtga cgagtgggta gacctggtca ccgcgccagc
    22261 gcgcaaagtg gtggagatcc gcaaaaccac cgtcggctgg gtgagccagt ttctgcgcgt
    22321 catcccgcgt atttcagcac tggaagtggt gatgcagccg ctgctcgata ccggcgtacc
    22381 gcgtgaagcc tgcgccgcta aagccgcgcg tctgctcacc cgcctgaacg tgccggaacg
    22441 cctgtggcac ctggcgccat caaccttctc cggcggcgaa cagcagcgcg tcaacatcgc
    22501 ccgcggcttt atcgtcgact acccgattct gctgctcgac gaacctaccg cctcgctgga
    22561 cgccaaaaac agcgccgcgg tggtggaact gatccgcgaa gccaaagctc gtggcgcagc
    22621 cattgtcggc atcttccatg acgaagccgt acgtaatgac gttgccgacc gcctgcaccc
    22681 aatggaagcc tcttcatgat tatcaataac gtcaaattgg tgctggaaaa cgaagtggta
    22741 cacggttcac tggaggtgca ggacggcgaa atccgcgcct ttgccgaaag ccagagccgc
    22801 ctgccggagg cgatggacgg cgaaggcggc tggctgctgc cggggctgat tgagctgcat
    22861 accgataatc tggataaatt cttcaccccg cgcccgaaag tcgactggcc cgcccattcg
    22921 gcgatgagca gccacgacgc gctgatggtg gcaagcggca tcaccaccgt actggacgcc
    22981 gtggcgattg gtgatgtgcg cgacggcggc gatcggttgg agaatctgga gaagatgatc
    23041 aacgccatcg aagagacgca gaaacgcggc gtcaaccgcg ccgagcaccg tctgctcctg
    23101 cgctgcgaac tgccgcatca caccacgctg ccgctgtttg aaaaactggt gcagcgcgag
    23161 ccggtgacgc tggtgtcgct gatggaccac tcgccgggcc agcgccagtt cgccaaccgc
    23221 gagaagtatc gcgaatatta tcagggcaaa tactcgctca ccgatgcgca gatgcagcag
    23281 tacgaagaag agcaactggc gcttgccgca cgctggtcgc agccgaatcg cgaatccatc
    23341 gccgccctgt gccgcgcgcg acaaattgcg ctcgctagcc atgatgacgc cacccacgcc
    23401 cacgttgccg aatcccacct gcttggcagc gtgatcgccg aatttcccac cacgtttgaa
    23461 gcggcggaag cctcgcgcaa gcatggcatg aacgtactga tgggcgcgcc gaatatcgtg
    23521 cgcagcggtt cgcactccgg caacgtggcg gccagtgaac tggcgcagct tggcctgctg
    23581 gatatcctct cttccgacta ctaccccacc agcctgctgg atgcggcatt tcgcgtcgcc
    23641 gatgaccaga gcaaccgctt tacgctgccg caggcggtga agttagtgac ccaaaatccg
    23701 gcgcaggcgc ttaatcttca ggatcgcggg gtgattggcg agggcaaacg cgcggacctg
    23761 gtgctggcac atcgcaagga caatcatatt catatcgacc acgtctggcg tcagggtaaa
    23821 agggtgttct gatgatggga aaactgattt ggttaatggg gccgtccggc tccgggaaag
    23881 acagcctgct ggcggaaatc cgcctgcggg aacaaactca gttactggtg gcgcatcgct
    23941 acattacgcg cgacgccagc gccggaagtg aaaaccatat cgccctgagc gagcaggagt
    24001 tttttacccg cgcggggcaa aatctgttgg ccttaagctg gcacgctaac agtctgtatt
    24061 atggcgtcgg cgttgagatt gatctctggc tgcacgccgg attcgacgtg ctggtcaacg
    24121 gctcacgcgc ccatctgccg caggcgctgg cgcgctatca atcggcgctg ctgcccattt
    24181 gcttactggt ttcgccggag atcctgcgcc agcgcctcga aaaccgtggt cgtgaaaatg
    24241 ccagtgaaat taacgcccga ctagcacgcg ctgcccgcta tactcctcag gattgtctta
    24301 cgctcaataa tgacggcagc ctgcgccagt cggtcgacaa gctgctgacg ctgattcatc
    24361 agaaggagaa acaccatgcc tgcttgtgag cttcgcccgg ctacgcagta cgacaccgac
    24421 gcagtttacg cgctgatttg tgagctaaaa caggcggagt ttgaccacca tgcgtttcgc
    24481 gtgggcttta acgccaacct gcgcgaccca aacatgcgct accatctggc gctgctggat
    24541 ggcgaagttg tcagcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg
    24601 atcggcgaaa ttcaggaact ggtggtgatg ccgcaggcgc gcggtctgaa cgtcggcagt
    24661 aaattactgg catgggcaga agaagaagcc cgccaggccg gggccgaaat gaccgcgctt
    24721 tcgaccaacg tgaaacgcca cgacgcgcac cgtttctatc tgcgagaagg ctacgagcag
    24781 agtcacttcc gcttcaccaa ggtgctgtaa catgagcctg accctcacgc tcaccggcac
//
LOCUS       DAJTYI010000049        24103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYI010000049 DAJTYI010000000
VERSION     DAJTYI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            62..343
                     /locus_tag="OO564_001949"
     CDS             62..343
                     /locus_tag="OO564_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6653091.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNERQGRDSPLMEKLKHAAEKIRSQFA"
     gene            309..1386
                     /locus_tag="OO564_001950"
                     /pseudo
     CDS             309..1386
                     /locus_tag="OO564_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1779..2120)
                     /locus_tag="OO564_001951"
     CDS             complement(1779..2120)
                     /locus_tag="OO564_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6653092.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2133..3005)
                     /gene="copD"
                     /locus_tag="OO564_001952"
                     /pseudo
     CDS             complement(2133..3005)
                     /gene="copD"
                     /locus_tag="OO564_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3009..3383)
                     /gene="yobA"
                     /locus_tag="OO564_001953"
     CDS             complement(3009..3383)
                     /gene="yobA"
                     /locus_tag="OO564_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6653093.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3522..3752
                     /gene="holE"
                     /locus_tag="OO564_001954"
     CDS             3522..3752
                     /gene="holE"
                     /locus_tag="OO564_001954"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6653094.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3854..4510
                     /locus_tag="OO564_001955"
     CDS             3854..4510
                     /locus_tag="OO564_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6653095.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4534..5196
                     /gene="exoX"
                     /locus_tag="OO564_001956"
     CDS             4534..5196
                     /gene="exoX"
                     /locus_tag="OO564_001956"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6653096.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5193..7253)
                     /gene="ptrB"
                     /locus_tag="OO564_001957"
     CDS             complement(5193..7253)
                     /gene="ptrB"
                     /locus_tag="OO564_001957"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6653097.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7462..8121)
                     /locus_tag="OO564_001958"
     CDS             complement(7462..8121)
                     /locus_tag="OO564_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6653098.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8448..8804)
                     /locus_tag="OO564_001959"
     CDS             complement(8448..8804)
                     /locus_tag="OO564_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6653099.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO564_001960"
                     /pseudo
     CDS             complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO564_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9295..10473
                     /gene="purT"
                     /locus_tag="OO564_001961"
     CDS             9295..10473
                     /gene="purT"
                     /locus_tag="OO564_001961"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6653100.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10528..11169)
                     /gene="kdgA"
                     /locus_tag="OO564_001962"
                     /gene_synonym="eda"
     CDS             complement(10528..11169)
                     /gene="kdgA"
                     /locus_tag="OO564_001962"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6653101.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11206..13017)
                     /gene="edd"
                     /locus_tag="OO564_001963"
     CDS             complement(11206..13017)
                     /gene="edd"
                     /locus_tag="OO564_001963"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6653102.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13252..14727)
                     /gene="zwf"
                     /locus_tag="OO564_001964"
     CDS             complement(13252..14727)
                     /gene="zwf"
                     /locus_tag="OO564_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6653103.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15065..15934
                     /gene="hexR"
                     /locus_tag="OO564_001965"
                     /gene_synonym="yebK"
     CDS             15065..15934
                     /gene="hexR"
                     /locus_tag="OO564_001965"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6653104.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16062..17504
                     /gene="pyk"
                     /locus_tag="OO564_001966"
     CDS             16062..17504
                     /gene="pyk"
                     /locus_tag="OO564_001966"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6653105.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYVRRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17635..18606)
                     /gene="lpxM"
                     /locus_tag="OO564_001967"
                     /gene_synonym="msbB"
     CDS             complement(17635..18606)
                     /gene="lpxM"
                     /locus_tag="OO564_001967"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6653106.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18726..20048)
                     /gene="mepM"
                     /locus_tag="OO564_001968"
     CDS             complement(18726..20048)
                     /gene="mepM"
                     /locus_tag="OO564_001968"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6653107.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20064..20996)
                     /gene="znuA"
                     /locus_tag="OO564_001969"
     CDS             complement(20064..20996)
                     /gene="znuA"
                     /locus_tag="OO564_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6653108.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21075..21830
                     /gene="znuC"
                     /locus_tag="OO564_001970"
     CDS             21075..21830
                     /gene="znuC"
                     /locus_tag="OO564_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6653109.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21827..22612
                     /gene="znuB"
                     /locus_tag="OO564_001971"
     CDS             21827..22612
                     /gene="znuB"
                     /locus_tag="OO564_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6653110.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22759..23769)
                     /gene="ruvB"
                     /locus_tag="OO564_001972"
     CDS             complement(22759..23769)
                     /gene="ruvB"
                     /locus_tag="OO564_001972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6653111.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23778..>24103)
                     /gene="ruvA"
                     /locus_tag="OO564_001973"
     CDS             complement(23778..>24103)
                     /gene="ruvA"
                     /locus_tag="OO564_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="plays an essential role in ATP-dependent branch
                     migration of the Holliday junction; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6653112.1"
                     /translation="QFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLF
                     TPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRMVSKIARPDASSETLIREA
                     LRAAL"
BASE COUNT         5943 a         6138 c         5946 g         6076 t
ORIGIN      
        1 tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga gaggtatggc
       61 aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata acccaccatc
      121 aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac ctgaactaca
      181 cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata aggttgatac
      241 cgatataaca caccatcagc ctaatgagcg acaagggaga gacagtccgt taatggagaa
      301 gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta acatatcgaa
      361 ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt acactaggta
      421 aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat atatacaaaa
      481 actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt actatggcag
      541 actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga aatacttata
      601 aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt ttactgacag
      661 aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga gggaaaaaca
      721 cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc attgttgaag
      781 gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta gttacaagag
      841 aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa cttccggcat
      901 ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa gacataacga
      961 atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt aagacaggaa
     1021 tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga cttggtgcag
     1081 tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc cgggattaga
     1141 aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt tgtcgcagcc
     1201 agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga aatcagaagc
     1261 ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg tctacttggc
     1321 cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac gtacttactg
     1381 ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata accaaaaaag
     1441 accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt tgcaactaat
     1501 tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt ccagggaaat
     1561 ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta gttgccttgc
     1621 cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg gtcaataaaa
     1681 agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa gaactgaggc
     1741 ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt ttcagctcgt
     1801 tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc aatctgaatc
     1861 accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc gatatcgttt
     1921 aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc cggtcggcaa
     1981 gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc aaaacgactc
     2041 acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa cagaaacgcc
     2101 agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg
     2161 ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat ccacacaaac
     2221 cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata acgattagcc
     2281 agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag ttggccctag
     2341 taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac gccgcttgcc
     2401 agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc ctgaatagcc
     2461 tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag taatccccca
     2521 aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat tttcgctgtt
     2581 accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg cgcggtagtg
     2641 agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat caacgttacg
     2701 agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa aacagcctgc
     2761 caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc ctgaacggcg
     2821 agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa acgcagagaa
     2881 cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata cattgcaaag
     2941 ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca ggtaaacgcc
     3001 agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa
     3061 acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag tggaacaatc
     3121 agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt ttcgtttttt
     3181 ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa gtttaaagtg
     3241 attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg cgttaaatgc
     3301 gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag cgcgtagcga
     3361 agggagcgtg cagttgaagc catattatct attccttttt gtaataactt ttttacagag
     3421 cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca tccaggctca
     3481 ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag aatctggcta
     3541 aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc ggggtggctt
     3601 ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa cagccagaac
     3661 atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg gtcaatctgt
     3721 cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca ctttcttaca
     3781 taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca gtcattttga
     3841 aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac
     3901 ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg ccaggcaaca
     3961 atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt cgcgacgtgc
     4021 ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg caacgacctg
     4081 gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta ttcgtggaat
     4141 tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc atggtgcatg
     4201 tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag gtatggatat
     4261 ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg acctgctggc
     4321 gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc tgatggcaaa
     4381 tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc gcgccgatcg
     4441 tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg atatcattcc
     4501 attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga tacagaaacc
     4561 tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga cggaaaaatc
     4621 gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg
     4681 attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga tgtgatccca
     4741 cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg ccgcgtactg
     4801 cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt gtggccaggg
     4861 ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca gacaccgccg
     4921 ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt gcttatcgat
     4981 attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg acgtccgtcg
     5041 ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga cgttgccgaa
     5101 cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag cccggagctg
     5161 cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt gggtaatgtt
     5221 ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta cgatttaaag
     5281 cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa aagatggtca
     5341 tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata ttgcacctga
     5401 gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt gacgttgtca
     5461 tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt tccccactct
     5521 tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac aacatcaaca
     5581 aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat tgcaacgccc
     5641 atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga gccatagccc
     5701 agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt cttcttcaga
     5761 aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac atggacaatg
     5821 gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc ggcatcaata
     5881 cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt gcgaaaatgt
     5941 ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac tatccacagg
     6001 tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt taatacacga
     6061 cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat ggaagaataa
     6121 ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt cacataggcc
     6181 ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg
     6241 gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt aaacccttcc
     6301 agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc acgcatacgg
     6361 gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa acgatgctgg
     6421 tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa cggctcggca
     6481 tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc cagatgaatg
     6541 acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc gtctttttct
     6601 tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca gacctgataa
     6661 ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc atttgcccag
     6721 acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt ttccagatta
     6781 cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg
     6841 ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc tgcgcgcttg
     6901 ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga ttgacgctgg
     6961 tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc attttttatg
     7021 tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc
     7081 tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg ttgcaggtag
     7141 tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc
     7201 gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag catgttattg
     7261 ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac
     7321 agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac
     7381 agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa tagtcaactt
     7441 caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg
     7501 ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta
     7561 actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag
     7621 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
     7681 tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc
     7741 gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa agaccagcgc
     7801 aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
     7861 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
     7921 cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga
     7981 tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta acagtttgcc
     8041 caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag
     8101 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
     8161 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgc
     8221 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat caggtgataa
     8281 gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc
     8341 gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg gacggacaag
     8401 gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
     8461 tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc ggcactttta
     8521 ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg
     8581 acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca gcgcgccacg
     8641 cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc cagaccttca
     8701 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
     8761 gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
     8821 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
     8881 tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg taatttgccg
     8941 gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga aagggcttca
     9001 cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag cagatccgcc
     9061 gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct
     9121 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
     9181 ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca
     9241 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
     9301 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
     9361 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
     9421 cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
     9481 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
     9541 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
     9601 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
     9661 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag ccttttccgc
     9721 gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc
     9781 aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga atacgctcag
     9841 caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
     9901 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accagtaggt
     9961 catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt
    10021 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt
    10081 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct
    10141 cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga gtttgccctg
    10201 catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg tcctgcagct
    10261 tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
    10321 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
    10381 cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga acgcgcgaag
    10441 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct acaaaaatgc
    10501 ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag cttcacgcgc
    10561 cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa ccagccagga
    10621 accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag ccggagaaat
    10681 accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct gcagggcttt
    10741 cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt agtccatacc
    10801 cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc cttcggtagc
    10861 agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac ccgcttcagt
    10921 gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg cttcaggcac
    10981 ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag tcacttccag
    11041 aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt ccagtttttt
    11101 taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac ttgttttcca
    11161 gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca
    11221 ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata attcacgtcc
    11281 tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag ccagttccgc
    11341 ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga tgtccccgtc
    11401 gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga tagctgacgg
    11461 cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt tgaaacaccg
    11521 gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc cgttcgcttt
    11581 tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg cttcaaaggc
    11641 cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca cctggttctc
    11701 aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta acacttttgt
    11761 cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt cgagtgattt
    11821 ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa gggtataacg
    11881 aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg ctttgagcag
    11941 ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat cggccggacc
    12001 gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga agtcatccca
    12061 gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt ggttagtgga
    12121 accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat cgatcatctt
    12181 accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac gcgcagcggc
    12241 agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc ctggcaactg
    12301 catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga aagtacatgt
    12361 tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt ccactttacc
    12421 ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg
    12481 tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca tcgtcagacc
    12541 cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt tatgggacag
    12601 ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt ccattccatc
    12661 ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct gaccaaccgc
    12721 attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag gctggtgcgc
    12781 ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa
    12841 agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac cgcatgccaa
    12901 ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat aagcagagcg
    12961 agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg gattcataaa
    13021 ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat tcagaggatt
    13081 tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt cagtgtaata
    13141 aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc
    13201 tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat attactcaaa
    13261 ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg gtccccaggt
    13321 tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag taatggagtc
    13381 tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct gaataccacg
    13441 catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat taaaggtttc
    13501 tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa ggccaggaac
    13561 tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag tcagtttatt
    13621 ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt tgaaatagac
    13681 cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga atggcacacc
    13741 ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg tattgctact
    13801 tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg cgaagcccgc
    13861 agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc ggcgcaggga
    13921 cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag atggcggaga
    13981 catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac gcatctgacc
    14041 ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca cggtaatctc
    14101 aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag caaaacgcag
    14161 cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt aaacctggca
    14221 ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca gcgacgtccc
    14281 cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct cgccaagccc
    14341 tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag tgatacgatt
    14401 tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga catcgagatt
    14461 acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg tttctttcat
    14521 gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc agtcagcacg
    14581 ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca gttgatacag
    14641 ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga ccaggtcaca
    14701 ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa cccggtactt
    14761 aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc gagcttgcga
    14821 aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag tgcggcaaaa
    14881 ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca tgttatgaaa
    14941 aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc gtaatatctg
    15001 acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg agtctcttta
    15061 catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca aatcagagcg
    15121 caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga gtattgctgc
    15181 tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga
    15241 cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga atggcactcc
    15301 ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg ggaaaatatt
    15361 tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat ctgccatcaa
    15421 ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat taggctcttc
    15481 tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc cggtggtgta
    15541 ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt
    15601 ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc agctggcacg
    15661 cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg cccgggaagc
    15721 aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc ccatggtttc
    15781 tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt tgcgacgcgg
    15841 tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga
    15901 taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt cctaatctta
    15961 tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt cggatttcat
    16021 gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa aggcttcgca
    16081 gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat cttgaaaaag
    16141 ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg cctgaagatc
    16201 acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt catgtggcta
    16261 ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa ggcaaagttt
    16321 tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt gaaggcgaca
    16381 aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct ggtgacatcc
    16441 tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc atgaaagtgt
    16501 tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac aaacttggcg
    16561 gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag actgcggcgt
    16621 tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat ctgaactatg
    16681 tccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag gttgaacgtg
    16741 cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct gacgtggtaa
    16801 tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc ggcattcaga
    16861 aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg acccagatga
    16921 tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg
    16981 ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct gggcagtatc
    17041 cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa atcccgagca
    17101 tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa gctattgcca
    17161 tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt accatgaccg
    17221 aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca attttcgcca
    17281 tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt acgccggtgc
    17341 actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat ctgctgcgcg
    17401 ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac gtgatgagta
    17461 ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt tgccggatgc
    17521 ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt cgaatattct
    17581 gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc ttttttattt
    17641 gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc gagttttcag
    17701 caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct cgacttcttc
    17761 attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca tcggtgggcg
    17821 cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg gtacaacgcg
    17881 cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat aggtggcaaa
    17941 gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat aatatcccca
    18001 gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg catgcagacg
    18061 accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt tgccctgatt
    18121 atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag gaatatcgac
    18181 ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca tctcttcgat
    18241 gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc gtattgccaa
    18301 ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa caatcgcttc
    18361 gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg cacgacggcg
    18421 tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg gatcacggaa
    18481 ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg ctccccagta
    18541 gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat tttttttcgt
    18601 ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt gtagcggcgc
    18661 aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct gcgaatggat
    18721 gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag gaattcgcga
    18781 cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg gtttacggcc
    18841 tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc ggtattaccg
    18901 gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag aatcttgcgc
    18961 aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata ataacctgct
    19021 gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac tggcgtacct
    19081 tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac cggattagtt
    19141 cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa tcgcaagaat
    19201 cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc gcggattgcg
    19261 taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg ctcacgttta
    19321 ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt cagtttgcgg
    19381 aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct ggttaaaccg
    19441 gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag attgttaacc
    19501 cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt acggtcatag
    19561 gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc gtccgcggtt
    19621 aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc ggcagcagcg
    19681 agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct tagcgtatcg
    19741 ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc atccagttcg
    19801 tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact ggcttcaggt
    19861 aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg cgtggcatca
    19921 cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt gagcgacccc
    19981 aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga gcgggctatc
    20041 tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata ctggttggct
    20101 aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt ccccagggga
    20161 tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac cgctggcctg
    20221 aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt tcttatttca
    20281 tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc aagcggtgtc
    20341 agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata acctttcccc
    20401 ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg tgcctcaaaa
    20461 tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac taattttcca
    20521 tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg catgttgaaa
    20581 tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc atcatcgccg
    20641 tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt tacctgcttc
    20701 gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg gccaacccaa
    20761 acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga ataatcatgt
    20821 tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc aatggcagaa
    20881 gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc ctgtgttgca
    20941 cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg taacataatg
    21001 cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat gactactgca
    21061 agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt tcttttggcc
    21121 aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt ttgactttac
    21181 ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg ctggtaacac
    21241 ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta ccgcagaagc
    21301 tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta cgccctggta
    21361 cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac ctgattaacg
    21421 caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg cgagcattgt
    21481 taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat gtaaatggcc
    21541 aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt ggcgttttaa
    21601 tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg tgcctgaatc
    21661 accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag tttatttcaa
    21721 tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat aatcatcgtc
    21781 acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga ttgaattatt
    21841 atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc tgggttcgtt
    21901 tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct cattacttgg
    21961 tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg ccgttacgct
    22021 gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga tcgacacgtt
    22081 attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta gtctgatgtc
    22141 taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag tgacgccaga
    22201 agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt tctggcaatg
    22261 gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg gtgtgaaatt
    22321 acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg tagcgatgaa
    22381 atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta ctgcgcgtcg
    22441 ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga tggtggcagt
    22501 aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt cggtggtgct
    22561 atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct aatctatcgc
    22621 tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc gctatctctg
    22681 gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt cggatgcggc
    22741 gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt gattccacgc
    22801 ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct gaatcaaata
    22861 aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg ccaggttatc
    22921 cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt tgcggtccat
    22981 ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag cgatatctgc
    23041 cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca gcagacggtt
    23101 ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt catcactcat
    23161 ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg gcacctgata
    23221 aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca gcgaacctgc
    23281 gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg agcgtgccgc
    23341 cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt acagcacttc
    23401 ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat gcggttcaag
    23461 gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag aagtcgtgcg
    23521 taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac ccaaccccgg
    23581 aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg ctttgatgaa
    23641 aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta atttagggcg
    23701 aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca gacggtctgc
    23761 ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa tgtttcactg
    23821 ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg tttatagcct
    23881 agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg gctggcagga
    23941 gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc tttaaatcgg
    24001 tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg cagtttcacc
    24061 agtgccccca cttcttcacg ctcaacggca ttaacgaact gct
//
LOCUS       DAJTYI010000050        24094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYI010000050 DAJTYI010000000
VERSION     DAJTYI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1217)
                     /locus_tag="OO564_001974"
     CDS             complement(<1..1217)
                     /locus_tag="OO564_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653113.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDA"
     gene            complement(1260..2645)
                     /locus_tag="OO564_001975"
     CDS             complement(1260..2645)
                     /locus_tag="OO564_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653114.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(2691..4727)
                     /gene="yihQ"
                     /locus_tag="OO564_001976"
                     /pseudo
     CDS             complement(2691..4727)
                     /gene="yihQ"
                     /locus_tag="OO564_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            complement(4926..5828)
                     /locus_tag="OO564_001977"
     CDS             complement(4926..5828)
                     /locus_tag="OO564_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6653115.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(5966..7207)
                     /gene="yihS"
                     /locus_tag="OO564_001978"
     CDS             complement(5966..7207)
                     /gene="yihS"
                     /locus_tag="OO564_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6653116.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(7224..8114)
                     /gene="yihT"
                     /locus_tag="OO564_001979"
     CDS             complement(7224..8114)
                     /gene="yihT"
                     /locus_tag="OO564_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6653117.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            9047..9943
                     /gene="yihV"
                     /locus_tag="OO564_001980"
     CDS             9047..9943
                     /gene="yihV"
                     /locus_tag="OO564_001980"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6653118.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            9977..10762
                     /locus_tag="OO564_001981"
     CDS             9977..10762
                     /locus_tag="OO564_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6653119.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            10861..11460
                     /gene="yihX"
                     /locus_tag="OO564_001982"
     CDS             10861..11460
                     /gene="yihX"
                     /locus_tag="OO564_001982"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6653120.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11454..12326
                     /locus_tag="OO564_001983"
     CDS             11454..12326
                     /locus_tag="OO564_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6653121.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12323..12760
                     /gene="dtd"
                     /locus_tag="OO564_001984"
     CDS             12323..12760
                     /gene="dtd"
                     /locus_tag="OO564_001984"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6653122.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            12805..13746
                     /gene="fabY"
                     /locus_tag="OO564_001985"
                     /gene_synonym="yiiD"
     CDS             12805..13746
                     /gene="fabY"
                     /locus_tag="OO564_001985"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6653123.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            14336..14674
                     /locus_tag="OO564_001986"
     CDS             14336..14674
                     /locus_tag="OO564_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653124.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            14844..15977
                     /locus_tag="OO564_001987"
     CDS             14844..15977
                     /locus_tag="OO564_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653125.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            16656..16874
                     /locus_tag="OO564_001988"
     CDS             16656..16874
                     /locus_tag="OO564_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6653126.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            17092..17334
                     /locus_tag="OO564_001989"
     CDS             17092..17334
                     /locus_tag="OO564_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653127.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(17664..18593)
                     /gene="fdhE"
                     /locus_tag="OO564_001990"
     CDS             complement(17664..18593)
                     /gene="fdhE"
                     /locus_tag="OO564_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6653128.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(18590..19225)
                     /gene="fdoI"
                     /locus_tag="OO564_001991"
     CDS             complement(18590..19225)
                     /gene="fdoI"
                     /locus_tag="OO564_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6653129.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(19222..20124)
                     /gene="fdoH"
                     /locus_tag="OO564_001992"
     CDS             complement(19222..20124)
                     /gene="fdoH"
                     /locus_tag="OO564_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6653130.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(20137..23187)
                     /gene="fdnG"
                     /locus_tag="OO564_001993"
     CDS             complement(20137..23187)
                     /gene="fdnG"
                     /locus_tag="OO564_001993"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(22600..22602),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6653131.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            23381..>24094
                     /gene="fdhD"
                     /locus_tag="OO564_001994"
     CDS             23381..>24094
                     /gene="fdhD"
                     /locus_tag="OO564_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6653132.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFA"
BASE COUNT         5994 a         5942 c         6232 g         5926 t
ORIGIN      
        1 gcatcgcttt gcgcgatgtt gggaacataa ccaatttgcg tcagcataat gcctggcaag
       61 aagccagcaa gtgcggcaga aattttacga aagaaggtat aaccggtata aacagaacct
      121 tcggcacgaa taccggtttt ccattcaccg taatctacgg tatctggtac cagtgcccag
      181 ttcaggctgt ttacaaaggc cgtgccaaaa aaggcgacac aagagaacat gacgaaagtg
      241 aaagagttac ttccccagaa ataattcagt atatcaccca ccgcccatag caccatgcca
      301 gctaaataaa cctgtttttt accaaaacat tttacagtta ctggcaccag taatacgccg
      361 acgaggatgc atcccatact gaagaatccc atccacgaca ataaattaat atcattcagc
      421 acatactggg tgtaatagac ctgaatcgcc agtttgatat taaatgccgc cagagtacac
      481 aggttagcaa tgcataaaac cagcaatggt ggattacgaa atatcgcgca aaaagatttg
      541 agaatactgg ctttatgtcc ggttggcgct gtgtccacat aatgctcttt gacgcctctg
      601 tagcacagca tcataaaaat aaagccgcca atggagaaca taagtgccgc acaggcataa
      661 ccgacggttg agtcagaaaa aagcgactgc aacggaataa acgccacggt acaaatcaat
      721 agccctatgg ttgcaccacc ctggcggtaa gccgcgagct gcgcgcgctc attcgggttt
      781 ttggtaattg ccgggatcat cgcaccatac gagcagttca tcagactgta tgaaagtcca
      841 aacatcataa acagcgcggt ggcaatcgtc gttttaaccg gcaaacaaaa ggtggtggcg
      901 ataaactgga gcgttgcaat taacgccgcc ggaactgctg catataaaat aaagggacga
      961 aatttgcctt taggcccaat atttttccgc gagtcgagta aaaatccggt gagcatatcg
     1021 gtaaacgcgg taaaaaactt cgcgaccaga aagataatcc cgccatagta agcaggcatc
     1081 cctaattcat cggtataaag tttgaggaga tacagagtac cgatgcagag cattaaattc
     1141 gaaccgacgt cgcccatacc ataggcgatt ttttcccgca gattcagttt taatgtcagt
     1201 ggattatggt cagacataat cactccttat aacgccccgc agggcgtcgt ttattattat
     1261 tacgccgtgc gtttacgggc ttctatctct tccacaatgc ggacatacat cttctcgttc
     1321 aggctgtaga agcaacccat cgccacaatg gtgactaccg ccagtgcgct tgggtagatg
     1381 aagatcaact ggcgtaaccc ttcaatggtg tggtcagcct gtgcgacgtt cggcacatag
     1441 ccaatttgcg tcagcatcca gccggggaag aaaccagcca gcgcctgaga cactttgcga
     1501 aagaaggtga agccggtgta gaccgtgcct tccgaacgca cgccggtgcg ccactcgccg
     1561 tactcgacgg tgtcggaaac cagcgcccag ttcaggctgt taacaaacgc tgagccgaag
     1621 aacgccaggc aggagaacgc cacaaagctg accgagccgc cgccgaagaa atagttgagc
     1681 agatcgccca gcacccaaat cagcaggccg ccgatataga ccttcttctt accaaaacgt
     1741 ctgactgcgc caggcatcag gaacacgccg atgaaaatac agcccatgct gaaaaatccc
     1801 atatacgaca acaggatggg atcgttaagt acgtactggg tgtaatagac ctgaatggcg
     1861 agcttgacgt taaacgcccc taaggtgcag aggttggcaa tacacagaat gaacagtggg
     1921 cggttaccgg caattgcgcg gaaagattgc aacaggccag gcttttgcgc cggattggtt
     1981 ggttgggttt cgacataacg ctctttcacg cctgagtagc agatccacat aaacagcagg
     2041 ccgaacagtg aaaacagcgt ggcggcgaag atatagccaa gtttctgatt accttcgata
     2101 agattcataa ctggcacgaa tcccaccgtg cacagcagca ggcccagcgt agcgccgccc
     2161 tgacgccatg ccgccagtga ggcgcgttcg ttggggtttt tggtgattgc cgggaccatt
     2221 gcgccgtagg agcagttcat catgctgaaa aacagtccgt aaagcataaa cagaatagtg
     2281 gccatcaccg ttttaccggt gacatcaaac ggtgtgccga caaagttggc gatcgccagt
     2341 aaggtgaccg ggaatgacgc atacagaata aaaggacgga acttaccttt tggaccgatc
     2401 ttgcgtcggg aatccaacat aatgccggta cccatatcgg taaacgcagt aaagaatttt
     2461 gaaatcaaaa agataatgcc gccataggtg cctggcagcc ccagaacgtc ggtataaaac
     2521 ttcaaaagat aaagcgtacc gatatccagc aggatgttag aggccaggtc gccaataccg
     2581 taagagagtt tctctttaaa gggcaggcgt aaagttgctg gatcttccgt tgtgatgtga
     2641 ctcatcgatg ttctcccttt gcccatgagt ttccccacgg gcggatgtga ttagatgctg
     2701 tttaacgacg cgaacagtgc cgcccattcg ctatcggcgc gataaaagac cggcggcttg
     2761 ccgatgggcg cattaacggt aacttccccg ccccggaacg cttcacccgt ccaggcatgt
     2821 acccagttat cctccggcag atagagcgtc cagtcgctgc gaccttcttc gtgtaccgga
     2881 gcgaccagaa tgtcgcggcc taacaggtac tgatatttca gggaataggt atgtgcatcg
     2941 tcttcgtaat gcaggaacag cgggcgcatg accggcagac cggacttcgc attcagcgcg
     3001 acggcctctt tcagataagg tttcagggtg gtgaaaacgg tagtcatacg ggcaaaatgg
     3061 gcgatagttt ccgcatcgcc gtcaaactgc cagttgtcgc caggacggtt accttcgtgg
     3121 gtgcgcatca tcggcgtgaa ggcgctgaaa tcgcaccagc gcagcagcag ctctttgctg
     3181 cgcttcatct caaacagggt ggtgtaaccg ccaatgtcgc tgtgatgcag accatgtccg
     3241 gtcattgcca gcgacagcgc cgccgggact acggacgcca ggccatcgtc gagactccag
     3301 tccacgttct ggtcgcccgc ccacatcatg gtggagtatt tctggctacc ggtagaaccg
     3361 gcgcgcataa agaagaggat ctcgccgagc ttgcctgttt cttcaagggc ttcgtagtta
     3421 cacttcgccc acagcgcagg ctaggcgtta tgcataattt cggcactgac gccgttatgc
     3481 aggtacgtgt cggtgggcag atactcgccg aagtcagcca tccagccgcc gcagccgagt
     3541 tcaatcatgt tctttttgat cacttccttg aaccaggcgt aggcttctgg attagtgaga
     3601 tcgacaacgc cgccgtaaaa ctcgccaaac tccaccagat agtcaccgcc agaggcatct
     3661 tttgctagat agccgtgttg tgccgcttct tcgcagagat ctttatcgct ggcaacatac
     3721 gggttgatat aggccaggaa ctgcacgccc tcctgattcc actgcttaat gcgtgaatcc
     3781 agttgcgggt agttttcgct gttccacttc cagttccaca tcacgcgctt gccaaaagag
     3841 gttatacgga tcccagacca gtcctgcgcc cagatgccgt taaccttcac gcccgcgtta
     3901 cgcatggtgt ccagtttctt ctggcacact tccgtcccgc catgaatgcc gagcgttacc
     3961 ccgtcataaa tccagtcggg cagttctggc tggcgtccca gcagggcagt taatttttcc
     4021 agcagggaga tgtatgtgtc agcacattca aaacgtagcg tagctttgtc ttcccacagc
     4081 gccagttcat ggtattccgg ggcgctaaag tcgaagttca tgtagcaact gttatcaaca
     4141 tggcagtaat acttctgcgt gctgacaaac gtaggctgtg ggaagaaagt ccagtaatag
     4201 tcgccgcccg cattttcttt gcagtcggcc tgccaggtga cataggtttg tttgttgcga
     4261 ccaacgcctt gttcactggt ccatagcggg aacggtttgc cgcgcagatc gaagtaggaa
     4321 aactgttcgc cgcagccgta gatatggtcc tctggttgag cggcaaggcg cagccagata
     4381 cggttgtggt taaggttgtc gttttgtagt tccagcaata aacggccctg atcgtcggca
     4441 gagatattca gcgtggcgct aatgtcagaa ccacggctga aatgaattaa ccaaccatcc
     4501 ggtgactggc tgacgatggc gtcggtaagc gcaattttct cctgtagttt atctttaatg
     4561 ctgaaattac cgcggaacat atcgatatcc gctatacctg agccaatcca taaacaagga
     4621 ttatctttgc tatgggttaa aataagacgt tgttgaaaat gtagggtaaa actgtcgtta
     4681 ttctgatgaa attgaaaatc taataactgt ggacgtggcg tatccataac taatctccat
     4741 agagttgaga acgagattgt tgccttcctg ttattacagg cttatgtaaa atgattctca
     4801 taaaactgat atataaaaac tttacagaag ctgagctgtt tttaacttaa taaattgaag
     4861 atacataatg ttgcctttag cgagtgaaat gttcttcaat gattttgact gctaatcaat
     4921 acaagttatt ttctctgtcc gtaaatatta aaaaacaggc ggtgcggttt tcctggttcc
     4981 agtaataata agtcgatacc tgaattaaag gcgttaggcg ggcaactcat aggttcaact
     5041 gccagtccct gacgctgtaa tttttcaccg ctataaacct gcacccaggg ttgatcggaa
     5101 caaaggctta ccgaaagcgc ctgctgtgga tgggtaatgg tcatttccca caaatcatta
     5161 gcggttttaa aggtgtggtc gatttttgtc gcggctattt ttttcgcctg cgtgaaattt
     5221 aaatccagct catcaacgtg gtgaagcgtt gtcggattta cgtgttcatc gacggcataa
     5281 acctggtttg ccggaagctg gaacagatat tcatcaacag atgttaagtt gcaggttaaa
     5341 taaggatgaa tacccacgcc ataaggcgct gcgacagtgc cgatattctg gcttgcgatc
     5401 tcaacggaaa gacccgtgtg tgcgttgagc gaataaacaa cttgtgaagc cagcataaac
     5461 ggatagccgt aactgggcgg cagaaaagct gtcagggtaa cgctggtggt ggttaattca
     5521 ctaatttgcc agtctcgcca ggcaagcagc ccgtgaatag cggcttttga actatgttca
     5581 ttaatgggta actgatattc ctgcccctga taacggtaac agccgttggc gatccggttc
     5641 ggccacggaa taagcacttt acccagatgc gccagcggca tctcttcggg tttgtgcggg
     5701 atgactaaat gacaaccctg aaaggttaat tcagccagac cagcaccgac ggtcacaatg
     5761 gtcgcgtgat aatcccccgc ggcaagtgaa acggtctgtc cactgcaatg catattggtg
     5821 atttgcatac aaggcacctt aattaacgac atacagcctc ccgtgaggct gatgtgaaac
     5881 ccaaaaactg caccggacgg tcccctcgcc cctttgggga gagggttagg gtgaggggaa
     5941 aacccgcacc ctattttcag atcagttatt tcgcattaat atccagcaga cccgctgcaa
     6001 ccgctggagc catgccgggc gctaacggga tacgcgggat caccaggcaa tgcagcaggt
     6061 gataaatatc ctgtttgccg tcccagactt tggtgatgac cttattgtcc gcatccagct
     6121 cctgccacca ggaaccattt tcatagtcca tcaggtactt aatgcagtac tcccaccatg
     6181 tttgatacca ggtttcatac tggcgatcgc cagtgacggt gtagagcgcg taggccgtac
     6241 ccattgcttc gacgataggc caacgtacac gttcgcggac caccggtttt ccttcccagt
     6301 caacggtata aacaatcccg tccgcgccat cgggtgccca ggcatcgcgc acggtggcgt
     6361 taaacagacc tttggcatct tctagcagcc atgctggtgg ttgttcgcaa cgggcttcca
     6421 gggcagcatg gatgtgcagc attaaacggc cccattcgat ccagtggcct ggtgtacctc
     6481 cgaacgcgcg gaagcgatgc gccgggttat ctttgttgta atccggcagc ggatcccact
     6541 gggtatcgaa gtgttcgtta acgcgataat gattatttct ggcgacgtcg tggataatca
     6601 cggaagccac gcgaatcgcg cgatccagcc attttttgtc gtgagtgacg tcataaacaa
     6661 tcaagaaagc ttccaccgcg tgcatattgg cattgccgcc gcggtactct tcggttttgc
     6721 tgaaggcttc gtcccaggat tccaggcaca tctgctcttc ttcgctccag aaatatttct
     6781 cgataatctc aatggtgtaa tcgagcagct tgcgcgcttc cgggtgaccc gttgtgacgg
     6841 cgctggcggc acccagcaga gcaaagaagt gttgatagcc ctgtttggag gcatccacca
     6901 cgccctcgtc attcacgcag gcataccagc cgccatattt tttatcacgc agtgcgccgt
     6961 tcatggcttt aataccgtga tcaaccaacg cgtaagcgcc aggtcgaccc atcgccgcag
     7021 caacggaata aacgtgcaac atacgagctg tgatccacag atgggtgccc atctcttctt
     7081 tgatttgccc tttattgcct aaccagccaa aaccagtcgg cactacggaa tttttaccaa
     7141 aatcaaagat gcggtcggtt tcctgttcca gccaacggtt gtggcttagg gtgttaaacc
     7201 atttcattcc ttttatcctc atcttagcgg cgtttagcca tcatttcgtc gacgatatcg
     7261 ccaagttgtt gtaatttcgg tgcgcaaacg tcacggagca tcagctcgtt gtctggtaaa
     7321 ccgacgaccg atgcccagac tgcacggcct gccaggaatc ccgatgcgcc cgccgtcatt
     7381 gccacgcgta cggcacgcgg gaacagtttt tcgtcgacac tggaagagag gatcacccat
     7441 ggcatattga tatggtcatt cagacgttgt gaagcacaga gaagctcttg ttgcggacct
     7501 ttgccataaa gcggcatttc aactttgtag aggtcagcgc cactgtcgcc cagctctttg
     7561 gcggcatcga tgaccgcttg ttcgcgatcg aatttatcgc cacgacgcgg tgggcggacg
     7621 actggctcaa tgatgcttac cagaccgtgt gagtggcaca gtccgttgaa ctctttcacc
     7681 atatccagac gttgctgcgc atcttcatca ctacgccaca gcaccagcag ttttaaggct
     7741 ttgccaccgt cttgtttgat ctgtagcgga ttgattttgc ggtcaataac cacgctatcg
     7801 accggaatac cgttgccagg aatgaactca tcggcggcga caatcatggc gcaacttttg
     7861 gcaatcgcgt tttgctcaac cacctggcgg tagcagaatt gttgatctac cagaatcgcc
     7921 gaggcataag gcgagagggc ctttgcagcg ttaactttga aatcagttaa aacgctatcg
     7981 gctaccggag cgggtgcccc agccgcggca aacatcatgc gcatggcttc gcgctgatcg
     8041 accgccagca tggcaaaacc gcccgatgcg cgcgcctggc tataaacctc ccgtgaggcg
     8101 gcccccagcg gcattggcac atgcagctgg ttggcgacat caagggcgat gccaagatcc
     8161 ttatgggcaa gatcgatcat gaaggcggga gaaagatctc cgctgaggac tttgtttggc
     8221 caggaagtgg tgaagtgacc tttaccggcg gcggtaccgc tcatcacttt gacggcaaca
     8281 tcgaagggaa gattcagggc ttcgcacaaa acggcggctt ctgccgaaag cgcattgagc
     8341 gcgatgctca tgtagttgtt gatgagctta acgcggatcc ccatgcccgg accgccagcg
     8401 ttgatcaact cactgcccat cgccatcagg atcggtgtgg cacgttcaac ttgttcagcg
     8461 gtgccgccag ccagcagtaa cagagtaccg gtaatggcat ttgcagaagt acggcctacc
     8521 ggaacatcca tcatgctgaa gcctttggct tgcatatcgg caatcaattt atcggtttgc
     8581 agcggatgga tggtggacat atcaatgacc agcgcatcgg tagataagcc ttcgcaaacg
     8641 ccgttttcac cgaacaacac gttgcgcacc agatcgccat tcggcaacat ggtaatgata
     8701 aattcggcat ctttagcggc ctgcaccggg ttggcggcgg gagtcgcacc tttgtctacc
     8761 agatgccgca cagcttcggc gttcacatca aagacgcgaa gttgatgccc ttgctgcaat
     8821 aaattgctcg ccattggcga acccatttgt cctaaaccga taaacgcgat tgctgccata
     8881 accctctcct gaatacagtg atgtcacttt ttgtcattta tgacatgctt tgcttgtctg
     8941 tttttgatcg catttgtaat ttatcgtcaa aaaattgaca gccgtcactt tttaaacaat
     9001 tggtgaaatg aaaatgaacg catcccaaaa tgtttaagga atgaccatga ttcgtgttgc
     9061 ttgtgtaggt ataaccgtga tggatcgcat ctattacgtg gaagggttac cgacggagag
     9121 cggtaaatac gtggcgagaa attatacgga agttggtggc gggccagcgg cgacggcagc
     9181 ggttgcggcg gcaaggctgg gggcgcaggt cgattttatt ggtcgcgtag gtgatgacga
     9241 cgcgggcaac agcctgctag cagagctgga atcctggggc gttaacaccc gttacaccaa
     9301 acggtataac caggcgaaat cttcgcaatc cgccatcatg gtggatacca aaggcgagcg
     9361 gataatcatt aactacccca gcccggatct gctgcctgac gcagagtggt tggaggaaat
     9421 tgatttctct cagtgggatg ttgtgctggc agatgtacgc tggcacgacg gcgttaaaaa
     9481 agccttcacc ctggcccgtc aggcgggtgt gatgaccgtt ctggacgggg acattacgcc
     9541 gcaggatatc agtgagctgg tggcattaag cgatcacgcg gccttttcag aaccgggtct
     9601 ggcgcgctta acgggcgtga aagagatggc cagtgcgcta aaacaggcac aaacgctcac
     9661 aaatggtcat gtctatgtga cccagggtag cgcaggctgc gactggctgg aaaatggtgg
     9721 gcgtcagcat caaccggcct tcaaagttga tgtggtagat accacaggtg cgggtgatgt
     9781 ttttcacggc gctttggcgg tggcgctggc aacaagtggg gatttagcgg agtcagtccg
     9841 cttcgccagc ggtgtagcgg cgttaaaatg cacacgtccc ggtggacgag ccgggatccc
     9901 tgactgtgat caaacccgat cttttttgtc actttttgta taaaatgcca gggtgatggt
     9961 ttttcgagga attttcatga gccttaccga actaaccggt aacccgcggc acgaccaact
    10021 cctcatgctg atcgccgagc gtgggtatat gaatattgat gagctggcaa atctgctgga
    10081 tgtctccacg cagacggtcc gccgggatat tcgtaaatta agcgagcaag gcctgattac
    10141 gcgccatcac ggtggcgcgg gtcgggcttc cagcgtcgtt aatacggcgt tcgagcagcg
    10201 tgaggtttcg caaaccgaga aaaaaaaagc gattgccgaa gcggtggcag actatattcc
    10261 tgatggttca acaatattta tcaccattgg tacgactgtt gagcatgttg cccgggcgtt
    10321 acttaaccat aatcatttgc ggataatcac caacagcctg cgtgtggcgc atattcttta
    10381 ccacaacccg cgctttgaag tgatggtgcc cggcggtacg ttgcgctctc ataatagcgg
    10441 gatcattggc ccttcagcgg cgtcctttgt ggctgatttt cgtgctgatt atctggtaac
    10501 aagcgttggg gcgattgaga gcgatggcgc gttgatggag tttgatgtaa acgaagctaa
    10561 cgtggtgaaa acgatgatgg cgcacgcgag aaatattctg ctggtcgccg atcacactaa
    10621 gtatcatgct tcggcggcgg ttgaaattgg taacgtggca caggtcactg cgctctttac
    10681 cgacgagctg ccgcccgctg cgctaaaatc acgcttacaa gacagccaaa ttgaaatcat
    10741 tcttccccag gaagacgcgt agatttttgt gaccgttaac ctggcttcat accttgccac
    10801 atagccaaac ccatcctttc ccgctacagt taatttcttg tggcgcgaaa ggaggcaaaa
    10861 atgctctata tctttgattt aggtaatgtg attgtcgata tcgactttaa ccgtgtgctg
    10921 ggagcctgga gcgatttaac gcgtattccg ctggcatcgc ttaagaagag ttttcatatg
    10981 ggggaggcgt ttcatcagca tgagcgtggg gaaattagcg acgaagcgtt cgcagaggcg
    11041 ctgtgtcatg agatggctct accgctaagc tacgagcagt tctctcacgg ctggcaggcg
    11101 gtgtttgttg cgctgcgccc ggaagtgatc gccatcatgc ataaactgcg tgagcagggg
    11161 catcgcgtgg tggtgctttc caataccaac cgcctgcata ccaccttctg gccggaagaa
    11221 tacccggaaa ttcgtgatgc tgctgaccat atctatctgt cgcaagatct ggggatgcgc
    11281 aaacctgaag cacgaattta ccagcatgtt ttgcaggcgg aaggtttttc acccagcgat
    11341 acggtctttt tcgacgataa cgccgataat atagaaggag ctaatcagct aggcattacc
    11401 agtattctgg tgaaagataa aaccaccatc ccggactatt tcgcgaaggt gttatgctaa
    11461 aaaccattca ggacaaagcc aggcaccgta cccgtccact atgggcctgg ctaaaactac
    11521 tctggcaacg tattgatgag gacaacatga caaccctggc aggtaacctt gcctatgtgt
    11581 cgttgctctc attagtgccg ctggttgccg ttgtttttgc gcttttcgcc gcttttccca
    11641 tgttttccga cgtcagcatt cagttacgtc actttatttt tgccaacttt ctgcctgcta
    11701 ctggcgatgt tattcagcgg tatatcgaac aatttgttgc taattccaac aagatgaccg
    11761 ccgttggggc gtgcgggctg atcgtcaccg cgttattgtt gatgtactcc atcgatagcg
    11821 cgttgaatac catctggcgc agtaaacgag cgcgaccaaa aatttactcg ttcgccgtgt
    11881 actggatgat tttaacgctg gggccgctgc tggcaggggc cagtctggcg atcagttcct
    11941 atttgctctc cctgcgctgg gcgagcgatc tcaatactgt catcgacaac gtgctgcgta
    12001 tttttccgtt gctgttgtcg tggatctcct tctggttgct gtacagcatt gttcctacca
    12061 tccgcgtacc taaccgcgac gcgattgtcg gcgcgtttgt cgccgcactc ctgttcgaag
    12121 caggaaagaa aggtttcgcg ctttatatca ccatgttccc gtcatatcag ctcatttacg
    12181 gtgtgctggc ggtgatcccc attctctttg tttgggtcta ctggacatgg tgtatcgtct
    12241 tgcttggcgc ggaaattact gtcactctcg gggaataccg caaactaaaa caagcagctg
    12301 aacaagaaga agacgacgaa ccatgattgc attaattcaa cgcgtaaccc gtgccagcgt
    12361 caccgtggag ggagaagtga cgggcgaaat tggcgcggga cttttggtgt tattgggtgt
    12421 cgaaaaggat gacgacgaac agaaagcaaa ccgtctgtgc gagcgtgtgc tcggctaccg
    12481 catctttagc gatgccgaag gcaagatgaa tctcaacgtg caacaggcgg gcggcagtgt
    12541 actggtggtt tcccagttta ccctcgccgc agataccgaa cgggggatgc gcccaagttt
    12601 ctccaaaggc gcatcaccgg atcgcgcaga ggcgttatat gactatttcg tcgaacgctg
    12661 ccgccagcaa gagatgaaca cgcaaacagg acgcttcgct gccgatatgc aggtatcgct
    12721 ggtcaatgat ggccccgtga cattctggtt gcaggtatga gccagcttcc agggttgtca
    12781 cgggaaacaa gagagagtat cgctatgtat caccttcagg ttccacaaac agaagaagag
    12841 ttagagcgtt actatcagtt tcgctgggaa atgttgcgta agcccctgca tcaaccaaaa
    12901 ggttcggaac gcgacgcgtg ggatgcgatg gcgcatcacc agatggtcgt cgacgagcag
    12961 ggtaatctgg tggcggtagg ccgattgtat attaatgccg acaatgaagc gtccattcgc
    13021 tttatggccg ttcacccgga cgtgcaggac aaagggttag gaacgctgat ggcgatgacc
    13081 ctggagtcgg tggcgcgtca ggaaggcgtt aagcgcgtga cctgtagcgc ccgtgaagac
    13141 gcggtggagt ttttcgccaa gctggggttt gttaatcagg gagaaatcac cacgccaacc
    13201 accacgccga ttcgccattt tttgatgatt aagcccgtcg ccactctgga tgatattttg
    13261 catcgcggcg actggtgcgc gcagctgcaa caggcgtggt acgaacatat cccgcttagt
    13321 gaaaaaatgg gcgtgcgtat tcaacaatat accgggcaaa aatttatcac caccatgccg
    13381 gaaaccggca atcagaatcc gcaccatacg ctgtttgccg ggagtttatt ctcgctggcg
    13441 acgctcaccg gttgggggct tatctggctg atgctgcgtg aacgccacct cggcggaacg
    13501 attattctgg cggatgcgca tatccgctac agcaaaccga ttagcggtaa accccatgcg
    13561 gtagccgacc ttggtgcctt aagcggcgat ctcgaccgtc tggcgcgcgg acgaaaagca
    13621 cgggtgcaga tgcaggtcga aatctttggc gacgagacgc cgggtgcagt gtttgaaggc
    13681 acgtatatcg ttctgcccgc gaagccattt ggcccgtatg aagagggcgg gaacgaagaa
    13741 gagtagctta tggagagggg cggtgctgcc tctctcattc attccggtca tttacatcag
    13801 tccctgacta ttccggttat attgaataag aactttataa atttttattc ttcgttggca
    13861 ccatacgtgt cttgctacat cactgttcag gagcatttaa atattatttg tagagctgag
    13921 tggcaattat tgcagataat gcttaactca tcatcttagt tcaggtttga gcgatttttg
    13981 atagtcaaaa ccccctctgg tgccatactt ttttcataaa tatttttaaa atctatagct
    14041 cacaaacatt atgcaaagat aatgggctgg catacattgt cagtatcatt catttactgc
    14101 tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga aggaggctat
    14161 aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta atttagaaaa
    14221 acaactaatg agtaataaat caccgaagta aacgccatta aatcaatttt cactttgcct
    14281 taaaatggaa ggtgaggagc aactgtgcga ttgtgttaac gaactgaagc atggtttgtc
    14341 gagtaataat gaatatgacc tgtttaaaaa tagagaaacc tcactgtgga aacgcaaagt
    14401 accagaaagc gcagcgctgg caatgacggg aacagtatat aaattatttg gtaatcgctt
    14461 actcaacgcc cgcaatcagt tagataaaaa tctcctcgaa atggctatgc aaatggttta
    14521 tggcaaagat atggtgacaa agtccgggga tatattgatt gatatacaat taaataagga
    14581 tggcagtctc cagagtaagc actacacact tgatgtaggt gaagttataa acagttataa
    14641 tattgaccct ctttgtgaat gcttcaagta ttaatcaaac ttacttacat gataaaaatc
    14701 ctggtgacgt tattagcctg tatggcactt tagataacat tccccttgta gttaaggaat
    14761 gcacgggtaa aaatcatcaa attaatgtta aagccaaatt gccacaagaa cgaagtgaaa
    14821 aagttgagga tatgtgtgaa agaatgaaag gtggcatctc tatgtttaac cacaccacaa
    14881 aaacagctga taatatagag cataatttgc agctaagttt tctcgtgggt tcacgtccgt
    14941 cgatcaccaa taaatactca gcaaaagatg taggtccaga tatacttgca ttgcccgtca
    15001 acgtatatca tgcggatttt aaaattaaaa tcaataatgc agtggagaag gcttcatcaa
    15061 ttgtgttatg tgctgtgcaa aaaaatctcc atgcagcaag gaaggttctt agcgggataa
    15121 taggaaatgt gccaaaaaat aataaaccaa aaacaattat ttttgatttg cgttttctta
    15181 gtgcaattaa gcagaaagtt tttttgggca gtgaaaaacc gttgtttgta aagcataccg
    15241 caatgatgag ggaggcatgt aaggaattac cgcagtcagt ggaatatgtt cctttagcct
    15301 gtgaagcgca tagccatcgc agcgtcgccc ttgccaaaac cgtggctgcg cccatcatgg
    15361 cgacagttgt gggcttggca ttaatgtatg caacatcatc aggaccgtat tatgcgcaaa
    15421 gtctttctgg ttcgccggat atcgagatga taaaaaatcg catggctgaa ttgtttaacc
    15481 agtcggatat ttctgtgcgc aacaaatata gcccggcttt tgataaatgg aatgaactgg
    15541 tagataaatt aaatcacgaa agaaatacag ttccgtttgc gtgtttgacc accattttag
    15601 cgacggcgat aaatgaaact cttgaggggg atacgaatgt tgccgttgtg atggggtgta
    15661 aatctgcgaa agatcgcacg atatccattg tccttggcaa ctcgatgttg caaacactcc
    15721 ttgaaaaacg cctcgccgat agtggagaaa tagaaaatct ttttgatcaa cagggatatt
    15781 ttagttgcga ttcattaaca cccgaagaat taatgatgtt aaaaaacctt tttgatattc
    15841 gagttcttca tgtctctaac aaattcaatg ttggattgca aggtaatatc aacactgatg
    15901 ttttacaaga ttcgtttttt aaaaatgtcg atttcattca tgaatataac tcgtataccg
    15961 tagggatggg agtctgacga gtaaaactca ttaggattat cagaattcgg acaatgcatt
    16021 gcaaaaatga catacaaaga gagatgtgtg caggtactta gccgcgaggg gcatggcgaa
    16081 gccatacaga aagtaccagt attcagaact ccagtgaaag cggcatagtg gccgcctgct
    16141 caggagtttt gctatgaccc gtaatcgtgc tgaacgtcgc caccctatga tgcgattaaa
    16201 aaaactcatc gtcgcgctga tgattgacgt caatgctatc ggatgatgcc gggggtgttc
    16261 cggaaggaat tccttgtttc ccgaactgtt accaacaaaa ttataacgat gagcaagtaa
    16321 agaccggttg gtggcagtac tgaccggtct gtgatgaaag attaatttca ccttaaatcc
    16381 tggtgctgtt aggatgctga cgtttttcgg ctacaccatt cagattatgc aaataaatat
    16441 ccgtgaatat aatatattca tagcacccat aactcatcct gggcgataac taaaccattt
    16501 ttgagtaata aatgaaacaa ctgcaacgct cttattattt ctggaaaacg cctcgcaaaa
    16561 acaaaatgac atgcaaaaat ccgcattttt ttaaatgctt tttcggcgta attttcgcac
    16621 atacattaat gagtaatata tgtaaggtgg tggtaatggc gatgaatacg gtttttcttc
    16681 atttatcaga agaggcaatt aaacggctga acaagcttcg gggctggcgt aaggtttcac
    16741 gatctgcaat tttacgcgaa gcggttgagc aatatctgga gcggcagcaa tttccggtgc
    16801 gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg tgttgatgat cagtgtaagg
    16861 agcataagga atgaaaaaaa tttttcattc ttgaatataa aaaacagatg cccttattct
    16921 ggtattaata caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc
    16981 tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg
    17041 caagggtact tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct
    17101 ctggcaggta tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa
    17161 cttgatgatc tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta
    17221 gatcaatacc tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa
    17281 gggtgtgagg aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa
    17341 aaaatgatgt caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga
    17401 acgccttatc cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc
    17461 tgataagcgc agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt
    17521 atcgatgtgt tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg
    17581 taacgcggaa tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgacctg
    17641 cgccacccgg aaaacgtcag agattactct ccttcacccg gaaacaggaa cgggttaatg
    17701 gaactacggg cgtagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca
    17761 tctgccacgg cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg
    17821 cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc
    17881 agtttgccgc tctgctcgca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca
    17941 cacaggttgc agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac
    18001 accggcatag agccacatac cgggcaatat tgacgttgtt cgccgtattc agcgcgggct
    18061 ttgccgggga tcagattggc catctgcgcc cagtagagcg acagcgcagc ccagataaac
    18121 ggcgctttat cgctgctgac ggacgagaaa tcagaggcaa acagcgcgct ggccatatct
    18181 tccagctcct gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc
    18241 atttcaggtt tcagctcagc aattagcgcc atcagcagct tttgccagtg cttatcacgc
    18301 ggcagaacgt gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg
    18361 gtcagatcca tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc
    18421 gcagcaaagc gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg
    18481 gcgcggcggt tgtataaatt cttgagccga gggaacaata acggcggaat catattcgcc
    18541 gtacgtttct cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt
    18601 tcttttccgt tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg
    18661 cgctggtaac ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt
    18721 agatatgcac catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg
    18781 cgaatcggat caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc
    18841 cgctcaccag cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga
    18901 agttataacg cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg
    18961 cccaaaagat atcatcccga ttgattaggt tttgatgcca gtaacggaaa aacatgatga
    19021 tgaacgaggc aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg
    19081 tgcccatgat ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg
    19141 ccgccaggat gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc
    19201 gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt
    19261 ctcttcctca tccgcacggt tcggaccgac acccacgtag tggaagatac tggccgcaaa
    19321 ggtagccgca aagccaacag ccgcgagcgg tttccagatg cctttccaga atttcacggt
    19381 ttcgctgatt tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg
    19441 cagcacgtac atgacgtgtg taccaccgac gcctgccgga tcgtacagac ccgcattgtc
    19501 gtaaccgcgg gttttcagct cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt
    19561 cgtaccgaag tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac
    19621 caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg
    19681 cggaatgtcg aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg
    19741 gaagtcgacg ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag
    19801 gcagcctgga tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc
    19861 gttctgctcc acttccgaga agcgcattac cgtccacgat ttggcgctta aatcattggg
    19921 gttgtcgtac accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca
    19981 cgccacctga caggctttac agccgataca ggtggtaacg tcgatgagtt tcgccacttc
    20041 ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat
    20101 gatatcttga gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag
    20161 gaaggactta aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt
    20221 ggcaataaag ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt
    20281 atcgatatct ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc
    20341 tttgatatag ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt
    20401 attcgccagc gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag
    20461 cgcgtgtttg gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg
    20521 gaacttatcg gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt
    20581 cgagataacg tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg
    20641 ttccgggaac ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg
    20701 ctgcatgata aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc
    20761 ccagccggtc cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt
    20821 accctgcgga tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca
    20881 tgcccagccc agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg
    20941 gttgccttcc ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc
    21001 atcacgcaat tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt
    21061 tgccggatcg gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga
    21121 cttcggctca tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc
    21181 gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc
    21241 gtcagtcagc gtaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc
    21301 tgagttaacg attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc
    21361 ggtctggatc ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga
    21421 ggtttcagtg ttcagtgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat
    21481 cactttgttt ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt
    21541 gaccttgccc tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt
    21601 cggcaaccag tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt
    21661 catcatagag acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag
    21721 cggttttggc gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag
    21781 cgtcatgtaa cctggcagac tctgcgacag tagccccagg tccgtcagcc cctgaatatt
    21841 ggagtgaccg cgcagggcgt taacgccgcc gccggccatc cccatattgc cgagcagcag
    21901 ctggatcatc gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag
    21961 ggcatacagg aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac
    22021 tttcaggaac gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg
    22081 ggaaacgtgc tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg
    22141 tttggcgaag ccgttttcgt ccagttcata agtccaggag gatttatcgt acttgcgttt
    22201 ttccgcgtcg tagccggtga acaggccatc ttcaaagccg taatcctcac gcacgatcag
    22261 gctggcgttg gtataggctt cggtgtattc gtggttgaat ttttcattgt tcagcaggta
    22321 cagcaatacg cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc
    22381 agctaccgcc gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg
    22441 aattttggct tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc
    22501 catcaccacg acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg
    22561 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc
    22621 gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact
    22681 cgacgcggaa gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc
    22741 gttttgcgca atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa
    22801 cgcctcttcc caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa
    22861 cttcagacgg ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc
    22921 accgcggttg accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt
    22981 ttttgctccg tcaccgaggc tgaacatcaa cagcccacag cctacggaac aataggtgca
    23041 ggtattacgg gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac
    23101 gctgggtgca aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt
    23161 aaagaactgc cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc
    23221 atttacattc gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc
    23281 cgcaaaggtc cagaattggc tcaatttccc tccatctagg aagggtttgt aacagaatac
    23341 cataatgttg gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa
    23401 agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga
    23461 tgatttacaa cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta
    23521 caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct
    23581 cggtttttcg ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt
    23641 cgttccttcc tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg
    23701 gttgaaggag cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga
    23761 gcaacttaat gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct
    23821 caacaaactg gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac
    23881 tggttgtact cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga
    23941 agacgtgggt cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga
    24001 aagctggcag caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa
    24061 gtcggcgatg tgcggcgtag agattttgtt tgcg
//
LOCUS       DAJTYI010000051        23802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYI010000051 DAJTYI010000000
VERSION     DAJTYI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            200..388
                     /locus_tag="OO564_001995"
     CDS             200..388
                     /locus_tag="OO564_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6653133.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            400..1110
                     /gene="bcsQ"
                     /locus_tag="OO564_001996"
     CDS             400..1110
                     /gene="bcsQ"
                     /locus_tag="OO564_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6653134.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1107..3720
                     /gene="bcsA"
                     /locus_tag="OO564_001997"
                     /pseudo
     CDS             1107..3720
                     /gene="bcsA"
                     /locus_tag="OO564_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3731..6070
                     /gene="bcsB"
                     /locus_tag="OO564_001998"
     CDS             3731..6070
                     /gene="bcsB"
                     /locus_tag="OO564_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6653135.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6077..7183
                     /gene="bcsZ"
                     /locus_tag="OO564_001999"
     CDS             6077..7183
                     /gene="bcsZ"
                     /locus_tag="OO564_001999"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6653136.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7242..7418
                     /locus_tag="OO564_002000"
                     /pseudo
     CDS             7242..7418
                     /locus_tag="OO564_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            7746..9494
                     /gene="bcsC"
                     /locus_tag="OO564_002001"
                     /pseudo
     CDS             7746..9494
                     /gene="bcsC"
                     /locus_tag="OO564_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            9576..11564
                     /gene="hmsP"
                     /locus_tag="OO564_002002"
     CDS             9576..11564
                     /gene="hmsP"
                     /locus_tag="OO564_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6653137.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            11748..13034
                     /gene="dctA"
                     /locus_tag="OO564_002003"
     CDS             11748..13034
                     /gene="dctA"
                     /locus_tag="OO564_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6653138.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13255..14751
                     /locus_tag="OO564_002004"
     CDS             13255..14751
                     /locus_tag="OO564_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6653139.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14847..15776)
                     /gene="kdgK"
                     /locus_tag="OO564_002005"
     CDS             complement(14847..15776)
                     /gene="kdgK"
                     /locus_tag="OO564_002005"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6653140.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16008..16775
                     /gene="pdeH"
                     /locus_tag="OO564_002006"
     CDS             16008..16775
                     /gene="pdeH"
                     /locus_tag="OO564_002006"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6653141.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            16845..18905
                     /locus_tag="OO564_002007"
     CDS             16845..18905
                     /locus_tag="OO564_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6653142.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19087..20409)
                     /locus_tag="OO564_002008"
     CDS             complement(19087..20409)
                     /locus_tag="OO564_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6653143.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(20820..21833)
                     /gene="yhjD"
                     /locus_tag="OO564_002009"
     CDS             complement(20820..21833)
                     /gene="yhjD"
                     /locus_tag="OO564_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6653144.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(21882..22781)
                     /locus_tag="OO564_002010"
     CDS             complement(21882..22781)
                     /locus_tag="OO564_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6653145.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23301..>23802
                     /locus_tag="OO564_002011"
     CDS             23301..>23802
                     /locus_tag="OO564_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6653146.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALN"
BASE COUNT         5589 a         6014 c         6600 g         5599 t
ORIGIN      
        1 tcagatttag catgtgaatc aatttagaag gtcacaagac gtgtgatttt agtcttttcg
       61 tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt gagcaaattc ccgtctcgcc
      121 gtaaacaata aaccggcgaa atgctcacgg ttagaattgt atcatgaatg gtacggttat
      181 ttcataggga tcaagcaaaa tgaataacaa tgaaccagat actctgcctg atcccgcgat
      241 aggctatatc ttccagaatg atattgtggc gttaaagcag gcattttcac tgcctgatat
      301 tgattatgcc gatatttccc aacgcgaaca gttggccgcg gcattaaaac gctggccgtt
      361 gctggcagag tttgcgcaac aaaagtaggg gattggtgaa tggccgtact gggattgcag
      421 ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg cattagcctg gtcattacaa
      481 atgttgggag aaaatgtcct agtggtcgat gcctgcccgg acaacttgtt gcgcctgtca
      541 tttaatgttg attttaccca ccgtcagggc tgggccagag cgatgctgga tggccaggac
      601 tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt tgctgccttt tggtcagtta
      661 tccattgaag aacaagaaaa tccacagcac tggcaaaccc ggctgagcga tatttgctcc
      721 ggcttacagc aactaaaagc cagcgggcgt taccagtgga ttttaatcga cttaccgcgt
      781 gatgcctcgc agataaccca ccagctgctg agtttgtgcg agcactcgct ggcaatcgtc
      841 aatgtggatg ccaactgcca tatccgactg catcagcaag cgctgccgga tggcgcacat
      901 attttgatta atgacttccg tattggcagt caggttcagg acgatattta ccagctttgg
      961 ttgcaaagcc agcgccgatt actgccgatg ctcattcatc gtgatgaagc gatggctgaa
     1021 tgcctggcgg ctgaagagat actgacgctg gcgaactggt gcctgttgaa ctactccggg
     1081 ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac ccggtggttg cttatcccgc
     1141 cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt
     1201 tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt ccgctggagc
     1261 acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct gtatacgcca acgcctcgcg
     1321 tccgcgtccg ctggacccgg tccgttatct cattcaaaca tgctggttat tgatcggtgc
     1381 atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt caggtctgca aaatattcgt
     1441 ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat
     1501 ctggatgaaa aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt
     1561 atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc gtttaacccg
     1621 ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt acggcggatg
     1681 ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat
     1741 atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg
     1801 cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag
     1861 gtagtatggc cgctgaatcg tcagccggtg ccattgccga aggatatgtc gctgtggccg
     1921 tcagtggata tctttgtccc gacttacaac gaagatctca atgtggtgaa aaataccatt
     1981 tacgcctcgc tgggtatcga ctggccgaaa gacaaactga acatctggat cctcgatgac
     2041 ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgc
     2101 accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata tgccaaaggc
     2161 gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc
     2221 atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agactccaca ccacttcttc
     2281 tcgccggacc cgtttgaacg caacctggga cgttttcgta aaacgccgaa cgaaggcacg
     2341 ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt
     2401 tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg gcggcattgc tgtcgaaacc
     2461 gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc gtggctatac ctccgtgtat
     2521 atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag
     2581 cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc tcgataaccc gctcaccggt
     2641 aaaggactga agtttgctca gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg
     2701 ggcattccac ggctgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac
     2761 atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc tgccgcatat gatccatgcc
     2821 agcctgacca actcgaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac
     2881 gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac
     2941 aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg
     3001 gtgatctcgc ggccctacat cttccttgtt ctactcaacc tggtgggcgt tgcggtaggc
     3061 atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg
     3121 tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa
     3181 caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat
     3241 ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc
     3301 aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag
     3361 caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa
     3421 ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg
     3481 gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg
     3541 gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg
     3601 gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg
     3661 cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga
     3721 tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg ggatgagtgc
     3781 gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca atgctgagcc
     3841 agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgt caggcgagca
     3901 gggagctgat gcccccatcg tggcgcagaa cggtccttcg cgtgatgtga agctgacctt
     3961 tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt ggtattaacc cgaacggcag
     4021 cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca acctcgaata
     4081 cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga
     4141 gctgatgggc gtgctgccag tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat
     4201 gcccattaat ccactgttta ttaccgactt caaccgtgtg cggctggagt ttgtcggcca
     4261 ttatcaggac gtgtgcgaaa acccggccag caccacgctt tggctggatg ttgggcggag
     4321 cagtggactg gatctgacct atcagaccct gaatgtgaag aatgacctgt cacacttccc
     4381 ggtgccattc tttgacccgc gcgataaccg tactaacacc ttgccgatgg tctttgcggg
     4441 tgcgccggat gttgggctgc aacaagcatc tgccattgtc gcctcgtggt ttggttcgcg
     4501 ttctggctgg cgtgggcaga acttcccggt gctctataac caattgccgg atcgcaatgc
     4561 cattgtcttt gcaaccaacg acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa
     4621 agccccggtg attgagatga ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt
     4681 gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa ggtatcgctc agggtaacat
     4741 tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc
     4801 gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc acatttggcg aactgaaaac
     4861 ctatgaagaa cagttacaat ccagcggcct tgagccagca gcgattaacg tttcgctaaa
     4921 cctgccgccg gatctctacc tgatgcgcag taccggcatt gatatggata ttaattaccg
     4981 ctacaccatg ccgccggtga aagacagttc gcggatggat atcagcctga ataaccagtt
     5041 cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac cgtctgctgc tgcgtattcc
     5101 ggtattacaa ggtttgctgg atggcaaaac agatgtctct attccggcgc tgaaactggg
     5161 cgcgaccaac cagctgcgct tcgactttga gtatatgaac ccgatgccag gcggttcggt
     5221 agataactgt attaccttcc agccggtgca gaatcatgtg gtgattggtg acgattccac
     5281 catcgacttc tcgaagtatt accacttcat tccgatgccg gatctacgcg cctttgctaa
     5341 cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa
     5401 agcgcctaac gaagcacaga tggaaacgtt gctgaatact gttggtttta tcggcgcaca
     5461 gacgggcttc ccggcgatta atctgacggt gaccgatgat ggcagcacca ttcagggcaa
     5521 agatgccgac atcatgatca tcggtggtat cccggacaaa ctgaaagacg ataagcagat
     5581 cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt
     5641 ccccggcatt gtgccggacg agagcgatcg cgcggcagaa acccagtcaa cgctgacctc
     5701 ttccggtgcg atggcggcgg tgattggctt ccagtcgccg tataacgacc agcgcagcgt
     5761 gattgcgctg ttggcagata gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga
     5821 tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg atccgcgagt acggtatcaa
     5881 cagcctacgt gttggcgacg tttattacgt aggtcatctg ccgtggttcg agcgcttgtg
     5941 gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg gcggctatca gtgtgatatt
     6001 gctggcatgg gtactgtggc gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga
     6061 taacgagtaa ttgaagatga atgtgttgcg tagtggaatc gtgacgatgc tgttgttggc
     6121 tgcctttagt gttcaggcag cctgtacctg gcctgcctgg gagcagttta aaaaggatta
     6181 catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca ccacctccga
     6241 agggcaaagt tacggtatgt tctttgccct ggcggctaac gaccgtgcag ctttcgataa
     6301 tattctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac gtttgcccgc
     6361 ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca attcggcctc
     6421 cgatggtgat gtctggatgg cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca
     6481 gcgttatacc gacatcggca gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac
     6541 ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg ggttttgctg aggataacag
     6601 ctggcgtttt aaccccagct acatgccgcc gacgctggcg cagtatttca cccgctttgg
     6661 cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta ttgctggaaa ccgccccgaa
     6721 aggcttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc taaaagccga
     6781 aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag gcatgatgcc
     6841 tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg cgacattcac
     6901 tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag cgcagggtaa
     6961 aggaccggtc ggtttttctg ccgccatgct gcctttttta caaaaccgcg atgcgcaggc
     7021 agttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatacctatt acaactatgt
     7081 gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga caaaaggtga
     7141 gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt cacgctttcc
     7201 ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccatcagcag accctgcgga
     7261 tggacagcgg caacactaat gccgtgcgcg ggctggcgaa tatttaccgc cagcagtcgc
     7321 cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag ccagcggcgc agcattgatg
     7381 atatcgaacg cagcctgcaa aacgaccgtc tggcacagca ggcagaggta ctggaaaacc
     7441 agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg actggcgctg gaccccggca
     7501 gcgtatggat tacttaccga ctttcgcagg atctctggca ggccggacaa cgcagccagg
     7561 ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa caacccggag caggtttacg
     7621 cttacgggct gtacctctca ggtcacaacc aggacagagc ggcgctggcg catatcaaca
     7681 gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct ggttaatcga ctgcaaagcg
     7741 atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg caaagaggct gaagcggaag
     7801 cgatgctgcg tcagcaacca ccttccacgc gtattgacct cacgctggct gactgggcgt
     7861 aacaacgacg tgattacacc gccgcccgca ctgcatatca gaatgtcctg acgcgggagc
     7921 caactaacgc cgatgccatt cttggtctga cggaagtgga tattgctgcc ggtgacaaag
     7981 cggcggcacg tagccagctg gcgaaactgc ctgccaccga taacacctcg ctgaacactc
     8041 agcggcgcgt ggcgctggca caggcgcagc ttggagatac cgcagcggcg cagcagacgt
     8101 ttaataagtt gatcccgcag gcaaaatctc agccaccgtc gatggaaagc gcgatggtgc
     8161 tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc aaagcaggcg ctggaaacct
     8221 acaaagacgc catggtcgca tccggtgtga ctacgacgcg tccgcaggat aacgacacct
     8281 ttacccgact gacccgtaac gacgagaaag atgactggct gaaacgcggc gtgcgcagcg
     8341 atgcggcgga cctctatcgc cagcaggatc ttaacgtcac ccttgagcac gattactggg
     8401 gttcgagcgg caccggtggt tactccgatc tgaaagcgca cactaccatg ttgcaggtgg
     8461 atgcgccgta ttctgacggg cggatgttct ttcgcagtga tttcgtcaat atgaacgtcg
     8521 gcagtttctc cactaatgcc gatggcaaat gggatgacaa ctggggcacc tgtacattac
     8581 aggactgtag cggcaaccgc agccagtcgg attccggtgc cagcgtggcg gtcggctggc
     8641 gaaatgacgt ttggagctgg gatatcggta ccacgccgat gggcttcaac gtggtggatg
     8701 tggtcggcgg catcagttac agcgatgata tcgggccgct gggttacacc gttaacgccc
     8761 accgtcggcc catctccagt tctttgctgg cctttggtgg gcaaaaagac tccccgagca
     8821 ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt ggggctaagt ctgagctacg
     8881 ataaaggtga agtaaacggc gtctgggcat cgcttagtgg cgaccagtta accggtaaaa
     8941 atgtcgaaga taactggcgc gtgcgctgga tgacgggcta ttactataag gtcattaacc
     9001 agaacaatcg ccgcgtcaca atcggcctga acaacatgat ctggcattac gacaaagatc
     9061 tgagtggcta ctcactcggt cagggcggtt actacagtcc gcaggaatac ctgtcgtttg
     9121 ccataccggt gatgtggcgg gagcgcacgg aaaactggtc gtgggagctg ggggcgtctg
     9181 gctcgtggtc gcattcacgc accaaaacca tgccgcgtta tccgctgatg aatctgatcc
     9241 cgaccgactg gcaggaagaa gctgcgcggc aatccaacga tggcggcagc agtcagggct
     9301 tcggctacac ggcgcgggca ttacttgaac gacgtgttac ttccaactgg tttgttggca
     9361 cggcaattga tatccagcag gcgaaagatt acgcacccag ccatttcctg ctctacgtac
     9421 gttattccgc cgccggatgg cagggtgaca tggatttacc gccgcagccg ctgatacctt
     9481 acgccgactg gtaagttttc agatagcgcc tctcttaatg ccgctgcgat cgggtatact
     9541 cgggcggcaa tctgggattt ccggggggag acaatttgcg cgtaagtcgc tcgttaacaa
     9601 tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt cgtttttatt ttttgcaccg
     9661 ttttgctgtt ccatctggtc cagcagaatc gctataacac ggctacgcaa ctggaaagca
     9721 ttgctcgctc tgtccgcgaa cccttatctt cagctatttt gaaaggcgat attcccgaag
     9781 cggaagctat tcttgccagc attaaaccgg caggcgtggt cagccgtgcc gatgtagtgc
     9841 tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc agagcgtccg gtgccggtaa
     9901 tggttactcg cctgtttgag ctaccggttc aaatctcgct gggcgtctac tcgctcgaac
     9961 gtccggcaaa tccgcagcca atagcctatc tggtgctaca ggcggattcc ttccgtatgt
    10021 ataagttcgt gatgagcact ctctcaacgt tagtgaccat ttacttactt ttgtcgctta
    10081 tcctgaccgt cgccatcagc tggtgcatta accgcctgat tttgcatccg ttacgcaata
    10141 ttgctcgcga acttaacgcc atcccagccc aggagcttgt tggtcaccaa ctggcattac
    10201 cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg cagttacaac ctcaaccagc
    10261 aattgctgca gcgccattat gaagaacaga acgaaaatgc gatgcgcttc ccggtgtcgg
    10321 atttgccgaa caaagccttg ctgatggaga tgctggagca ggttgtcgcg cgtaaacaaa
    10381 ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga tactgcgggc gtgctgaaag
    10441 aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa actcaaatcg gtactgtcgc
    10501 cacgtatgat cctcgcgcag attagcggtt atgactttgc tgtcattgcc aacggtgtac
    10561 aggaaccgtg gcacgcaatc actttgggtc agcaagtact cactatcatg agcgagcgcc
    10621 tgccgattga acgtattcaa ctccgtccgc actgtagcat tggcgtggcg atgttctacg
    10681 gcgatctcac cgccgaacag ctttacagtc gcgctatttc tgcggcattt accgctcgcc
    10741 ataaaggcaa gaatcagatt cagttctttg atccgcagca gatggaagca gcccagaagc
    10801 ggttgacgga agagagcgat atccttaatg cactggaaaa tcatcagttt gcaatttggt
    10861 tacagccaca ggtcgagatg accagcggta aactggtcag tgcggaagtg ttactgcgta
    10921 tccagcaacc ggatggcagt tgggacctgc cggatggctt aatcgatcgc attgagtgct
    10981 gtgggctgat ggttaccgtc ggtcactggg tgctggaaga gtcctgtcga ttgcttgcag
    11041 cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa cctctctgcg ctgcaactga
    11101 tgcacccgaa tatggtggcg gatatgctgg aactgttaac ccgctatcgc attcagccgg
    11161 gaacactgat tctggaagtg acagaaagcc gacgtattga cgaccctcat gctgcggtgg
    11221 caatcctccg tccgctgcgc aatgccggag ttcgggtggc gctggatgat ttcggcatgg
    11281 gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt gccaatcgac gtactgaaaa
    11341 tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag catgattgct gcaattatca
    11401 tgctggcgca gagcctgaac ttacaaatga ttgccgaagg cgtggagact gaagcacaac
    11461 gagactggct ggcaaaagcg ggcgttggta ttgcccaggg cttccttttt gctcgcccac
    11521 tccctattga aatcttcgaa gagagttacc tggaagaaaa gtagctaccc caaactgatt
    11581 acaaaacttt aaaaagtgct ggtttgtgcg agccagctca aactttttaa cctttttgtt
    11641 tcaattatga tccaggtaca tttctgtgat gttgtctggg tgttatttta aggccgcagg
    11701 tacccccata accttacaag acctgtggtt ttactaaagg acaccctatg aaaacctctc
    11761 tgtttaaaag cctttacttt caggtcctga cagcgatagc cattggtatt ctccttggcc
    11821 atttctatcc tgaaataggc gagcaaatga aaccgcttgg cgacggcttc gttaagctca
    11881 ttaagatgat catcgctcct gtcatctttt gtaccgtcgt aacgggcatt gcgggcatgg
    11941 aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact gctttacttt gaaattgtca
    12001 gtaccatcgc gctgattatt ggtcttatca tcgttaacgt cgtgcagcct ggtgccggaa
    12061 tgaacgtcga tccggcaacg cttgatgcga aagcggtagc ggtttacgcc gatcaggcga
    12121 aagaccaggg cattgtcgcc ttcattatgg atgtcatccc ggcgagcgtc attggcgcat
    12181 tttccagcgg taacattctg caggtgctgc tgtttgccgt actgtttggt tttgcgctcc
    12241 accgtctggg cagcaaaggc caactgattt ttaacgtgat cgaaagtttc tcgcaggtca
    12301 tcttcggcat catcaatatg atcatgcgtc tggcacctat tggtgcgttc ggggcaatgg
    12361 cgtttaccat cggtaaatac ggcgtcggca cactggtgca actggggcag ctgattatct
    12421 gtttctacat tacctgtatc ctgtttgtgg tgctggtatt gggttcaatc gctaaagcaa
    12481 ctggtttcag tatcttcaaa tttatccgct acatccgtga agaactgctg attgtactgg
    12541 ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga caagatggag aaactcggct
    12601 gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta ctcgtttaac cttgatggca
    12661 catcgatata cctgacaatg gcggcggtgt ttatcgccca ggccactaac agtcagatgg
    12721 atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct ttcttctaaa ggggcggcag
    12781 gggtaacggg tagtggcttt atcgtgctgg cggcgacgct ttctgcggtg ggccatttgc
    12841 cggtagcggg tctggcgctg atcctcggta tcgaccgctt tatgtcagaa gctcgtgcgc
    12901 taactaacct ggtcggtaac ggcgtagcga ccattgtcgt tgctaagtgg gtgaaagaac
    12961 tggaccacaa aaaactggac gatgtgctga ataatcgtgc gccggatggc aaaacgcacg
    13021 aattatcctc ttaatctcac cacttatgcc cgcactccct tccccgagtg cgggtgtttg
    13081 cgcataattg acccctgtgc ctgaacttac cacctgggtg agttcaggtt tatttcactt
    13141 cttccgtgac attttcatgt tcttgcggtc taacacgaag tgtttttaac gtcatttaga
    13201 ctgaccgaca acgggcagtt tttttattac caggatagtt gatcaggggt tcacatgcag
    13261 ggcataaaaa ttcgactttt agcgggcggt ttgctgatga tggccactgc tggctatgtg
    13321 caggcagatg cgctccagcc tgatccagca tggcaacagg ggacgctttc caacggttta
    13381 cagtggcaag tgctgaccac cccccagcgt cccagcgatc gtgttgaaat tcgcctgctg
    13441 gttaataccg gttcgctcgc cgaaagtaca caacagagcg gttacagtca cgccatccct
    13501 cgtattgcgc taacgcaaag cggtggcctt gacgcagcac aggcgcgttc attgtggcag
    13561 caggggatcg accctaaacg cccgatgccg ccggtaattg tctcttatga caccacgctg
    13621 tttaatctga gtttgcccaa taaccgtaac gatttgctga aagaagcgct ctcttatctg
    13681 gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca tcaaccacgc gctgcaaagt
    13741 caggacatgg tggcaacctg gcctgccgat actaaagagg gctggtggcg ctatcgtctg
    13801 aaagggtcaa ccttgttagg tcacgatcct gccgatccgc tgaaacaacc cgttgaagcg
    13861 gaaaaaatta aagatttcta tcagaaatgg tacaccccgg atgcaatgac gctactggtg
    13921 gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca acaaaacgtt tggcgaactg
    13981 aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt ctccgctgcg tgcggaagcg
    14041 gtgagtatta tgactgacgc ggtgcgtcag gaccggttat ctatcatgtg ggatacgccg
    14101 tggcagccga ttcgtgaatc agccgcactg ctgcgctact ggcgtgcgga cctggcccgc
    14161 gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca gtgacagcaa agacatcggt
    14221 cttggatttg actgccgtgt gctgtatctg cgtgcgcagt gtgccatcaa catcgaatca
    14281 ccaaacgaca agctgaacag caaccttaat ctggtggcgc gtgaactggc gaaggttcgc
    14341 gataaaggtc tgccggaaga agagttcaat gcgttagtgg cgcaaaagaa actggagctg
    14401 cagaaactgt ttgccgccta tgcacgggct gataccgata ttctgatggg ccagcggatg
    14461 cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc agtatcagaa actgcggcag
    14521 gatttcctta acagcctgac tgtggagatg ttaaatcagg atctgcgtca gcagttgtcg
    14581 aatgatatgg cgttaatact gctgcagccg aaaggcgagc cggaatttaa catgaaagcg
    14641 ttgcaggcgg cctgggatca aatcatggcc ccatctaccg ccgctgcgac cacctctgtc
    14701 gccacggatg acgtacatcc tgaagtgacg gatattccac ctgcacagta atgcctgatg
    14761 gtgatgagtt agcacaacca tcaggtattt atggatgagc tggatagagg ggtaacactt
    14821 tatccagcct tttgcatatg ctgcgtttac gctggcatcg cctcacgcgg gataatcgcg
    14881 ccgcgatact gaataacggt acttgcggtc aggtgcccgc gtttcgccgc gttttccgca
    14941 ctgccgcccg tcaaacgtac cgccagataa ccggcgctga aagagtcgcc agctgcggtg
    15001 gtatcgatca ctttttcttt cggcagtttc accgccggaa catcaactaa cgcttcacca
    15061 gcaatggaca ccaggcaaga atccgccccg cgtttcacca ccacttcttt cacgcccgcg
    15121 ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac cccacagcgc gtcttcgtcg
    15181 tccagcgtca ggaaggcgat atccgtgcat tccagcattt gctggtacac ctgctgtgtc
    15241 tcttctttgc tggcccacag gcgcggacga tagttattgt cgaaaatcac ttttccgcct
    15301 ttggcgcggc attcgcgcag cagggaaagc agcttttcgc ggctggtcgg gcttaagatc
    15361 gccaggctaa tcccgctcag gtagagataa tcgaaattcg ccagctcttc gcaaatcgcc
    15421 gcagactgct cgctcgccag ccagaatttg gcggcggctt cgttacgcca gtagtagaac
    15481 gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc ccggaagacg gttttccatc
    15541 cgttgggtca gggaagtatc aacgttctcg ccgtgccagg cgtccagcat ctgctggcta
    15601 aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta atgccgcagg atcgacctga
    15661 cgggcgatat agacggaagt gttcagggta tcgccgccga aaccgcgctt aacgtccgcg
    15721 tctttctcgg aaagctcaat catgcattcg ccaatcacgg caatcttttt ggacatagtc
    15781 gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg tggtggtttg ctggtcaacc
    15841 atattaaaac agtgttccat tatttttttg agccagaacg tattcctgaa agattccgtt
    15901 gtggagtgag gaaatttacc ccggccaggc ggtaaagttc tgcccttacg cgccgataat
    15961 ctttgtcgag tccgggcagc atcactttta aacacaggac atctttgatg ataaggcagg
    16021 ttatccagcg aataagcaac cctgaagcaa gcatcgagag cttgcaggaa cggcgttttt
    16081 ggttgcagtg tgagcgtgct tacacctggc agccgatcta tcaaacatgc gggcggttaa
    16141 tggccgtgga gctattaacg gtggtcacgc atcccttgaa cccttcgcaa cgcctgccgc
    16201 cggatcgcta ttttactgaa atcaccgtca gccatcggat ggaggttgtg aaagagcaga
    16261 ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca cggcctgctg gcatcggtca
    16321 atattgatgg ccctacgctc atcgccctgc gtcagcaacc aaaaatcctg cgccagattg
    16381 agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat ccgtctgccg aaagattcaa
    16441 cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga tgattttggt accgggatgg
    16501 caaatttctc tgcactaagt gaagtgcgtt atgactacat caaaatcgcg cgagaactgt
    16561 ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt ttctcagctt ttacatctaa
    16621 tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga aacaccggaa gagtggcgtg
    16681 atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt tctttcacgc ccggcaccga
    16741 tggaaacgct gaatacggcg gttctggcgc tataagctgc ctcattttcc gccgggctgg
    16801 actatcttta ggactggcac aggaagaata tgaggaagcg aacgatgagc aaggcaggca
    16861 aaataaccgc tgcgatttca ggggttttct tgttgttgat tgtcgtggcg atcattttga
    16921 ttgcaacttt tgactggaat cgactcaaac cgaccatcaa ccagaaagtc tctgcggagt
    16981 tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt gtgggagcgg caaaaacaag
    17041 aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca tgcagaagac atcattcttg
    17101 gtaatccacc ggatattccc gaagtcacga tggtgcattt gccgcgagta gaggcaacgc
    17161 tggcaccgct ggcgctgctg accaaaacgg tctggctgcc gtggatcaag ctcgaaaagc
    17221 ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa ctggacgttt aatcttgcca
    17281 acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg gtcgtttcgg ctggataata
    17341 ttcttttcga tcaagggcgg atcgccattg atgacaaagt aagcaaagcg gatctggaaa
    17401 tttttgttga tccgttaggc aagccgctgc cattcagcga agttactgga tcgaaaggta
    17461 aagcggataa agaaaaggtg ggcgattaca tttttggcct gaaggcgcag ggacgttata
    17521 acggtgaacc gctcacgggt acgggaaaaa taggcggtat gctggcgctg cgtggcgaag
    17581 ggacgccgtt tccggtacag gctgatttcc gttcaggtaa tacccgtgtt gcttttgatg
    17641 gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt acggcttaaa ttttctggcg
    17701 attcactggg tgatctctat gaactgacgg gcgttctgct gcccgatacc ccgccgtttg
    17761 aaacggatgg tcggctggta gcgaaaatcg acactgaaaa atcgtcggtc tttgattatc
    17821 gcggttttaa tgggcgaatt ggcgatagcg atatccacgg ttctctggtc tacaccaccg
    17881 gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg gcaattgcgg ctggcggacc
    17941 tgggaccgtt gattggcgtt gattccggga aaggggcaga aaagtcgaaa cggtctgaac
    18001 agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt gctgccttat gaccgcttcg
    18061 aaaccgataa atgggacgtt atggatgccg atgttcgctt caaagggcgg cgcattgagc
    18121 atggcagtag cctgccgatt agcgatcttt ctactcatat catcctcaaa aatgctgacc
    18181 tgcgcctgca accgctgaaa tttggcatgg cgggcggcag cattgcggcg aatattcatc
    18241 tggaaggcga taaaaagccg atgcaggggc gggcagatat tcaggctcgt cgactgaaac
    18301 tgaaagaact gatgcccgat gtggaactga tgcagaagac gctgggggaa atgaacggtg
    18361 acgcggaact acgcggtagc ggtaactcgg tggcggcgct tttaggcaac agtaacggca
    18421 acctgaaact gttgatgaat gacgggctgg tgagccgcaa cctgatggag attgttgggc
    18481 tgaatgtcgg caactacatt gtcggtgcga tatttggtga cgatgaggtg cgggtgaact
    18541 gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg cccgcagatt tttgctttcg
    18601 atactgagaa cgcgttgatt aatgttaccg gcacggcaag ttttgcttcg gaacagctgg
    18661 atttgactat tgatccggag agtaaaggga ttcggattat cacactgcgt tcgccgctgt
    18721 atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa agccggaccg ctgattgccc
    18781 gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc tgcggcggcg ttactggcac
    18841 tgatctcacc ttccgaaggg gaggctaatc agtgtcggac gatattgtcg cagatgaaga
    18901 agtgatgata attgccggat gtctgacatc cggcaattaa acagatttag atttttggca
    18961 aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaatccttg
    19021 tgaattcaat atattgtatg gaaattcagt cctgataagc gtagcgcatc aggcttttca
    19081 cacttattac aacgactgat gtcgcgtctc atgggtcagc agcagggcga ttaacgtcaa
    19141 gccagccatc gccgccagat ataaccccac cgcacctaac ccgtagttag cctgcaacca
    19201 agctgcgata tacggcgcaa cggaagcccc gagaatcgac gatacgttgt aagagaacga
    19261 tgctccggtg taacgcactt ctgtcggaaa cagctctggt aacagcgcac ccatcggccc
    19321 gaaggtcagc cccatcagac ttaaccccag cagcaggaag gcaaaaacca gaatcgggtt
    19381 gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac aggatgatca gcgtggtgat
    19441 gatcaccatg cttttacggc gaccaaaggc atcagccagt aatccggcga ctggcaccat
    19501 cacgccaaaa ccaataactg ccatcatcag catccacaac acttcgttac gcggcaggcc
    19561 aagcccaact ggcgcggcgg cggtactgaa ggtcatagag tagaccgtca tgatgtaaaa
    19621 cagcgtatag gttgccagca taatgaacgt acccagtacg gttacgcgaa catgtttggt
    19681 cagcagcgta cccagcggga tcttcacctg ttttttcgct ttagcgactt tctcaaacac
    19741 cggcgactca tgcagcgaca cgcgaacata caggccgata atgaccagca ccgccgagaa
    19801 gataaatggc acacgccagc cccagctcat aaactgctca tcggtcagta gccaggaaag
    19861 cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt gcgcctagct gtgggaagga
    19921 gccatacagt gcacgtttgc gcggtggggc gttttcagtc gccagcagcg ccgcgccgcc
    19981 ccattcaccg cctaagccca gaccctgacc aaatcgagcc agcgccagca gcagtggggc
    20041 gaaaatacca atcgtggcat agcccggcag cagaccaatc accacggtcg aaatccccat
    20101 cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa aatgaccaaa
    20161 aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg aaggtggcga gcgactgtag
    20221 cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc ggaaacacaa taacggccgc
    20281 agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg ccaatgagag aggcgacaag
    20341 gactttatta cgcgagttga tcggcgtgta ttcttgttcg tggtcgagtg ttgtggctgt
    20401 tgcttgcata atcttttctt attttttggc taacgaatag ccatattacg cacagcaaaa
    20461 gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa aaccaggcaa aaaggggata
    20521 atgtttaggc cagtgatttt tcttctatga aatgcttcac ataattttat aaatagtgga
    20581 taaaactggt ttcaggttaa ataaaagtta ccaactggat ttatatgctg gggttatgag
    20641 ttggagtgaa attgtttgct cccaaataga tggctgaaga ggctctgggg aagagtttca
    20701 agaggttatg tgcgcattat agccggatgc gatgctggcg catcttatcc ggcctacggg
    20761 gagccaggcc gaataaaatc gtatgccgga taaggcgttt acgccgcatc cggcctattt
    20821 taaggctgcg ttttccctgg cattcgcggg tcgtctttat attcggcggt ggcaatccac
    20881 gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa atgccatcag ccccagcacg
    20941 gaaccaaatg ctgcgccaga gggggatttc atcagcgatg gcagggtgta ggtcatcacg
    21001 attttaatca cttcaaaacc aatagcagcc agaaatgttc cgcgaatcag ggcttttttg
    21061 cgcggacggt ggcgcggcaa ccgccagaag atccagaaga aaagcagata gttggcgaag
    21121 atggaaatcg ccaggccaat caatcgccac gtcggtttca gccactcaat gctgttcagg
    21181 tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa ccgaggtgat cgaaagcgtc
    21241 acaatcagcg caatcaacaa accaatcagt gaaataaaat cacgcagata ttttacccag
    21301 aacttctcct gatcttgcgg cgagcgttcc cagacatcgc gcgactgggc acgaatcgct
    21361 tcacgcaggt tacccatcca gttgatgccg gaataaagtg ccaccgccag gccgacaagc
    21421 cctacagtcg tacgctgctg aacggcggtg ttgatggtgt ttttcaacgt ggcggctagt
    21481 gtcggatcgc tgatgttttg cagaattttg tcgaagatat cctgtagcaa catcggatgg
    21541 gaggccagca caaagccccc tgcggcaaac gacaccatca aaatcgggat catcgacaaa
    21601 aatgagaaat aggtgatagc cgcaccaaac tggttaccca gccgatcgtt aaagcgttct
    21661 gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga ctttttcggc agtggtggtg
    21721 acggtttcca gcgcctggct aactttgccg cctttctcgc tattatccaa ctgcttaatc
    21781 ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt tttcctgcgt catccgctct
    21841 tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg gtcagtccag gtgctctttc
    21901 attacgccag ccagccactg cataaacagg tttacacgcc gggaaagctc ccgacgctgc
    21961 gggtaaacca gcgaaaggga gagcggctcg gcacggtagc ctggcaacac ttcaataagc
    22021 cgcccggcac gcagggcttc gcgcacggca atgcgcggga tctgaataat ccccagaccg
    22081 gcaagaccgg cggcgagata gttttcgcta ctgtttaccg tcaacatgcc gccagactta
    22141 aaccactgga cgccattaac gctggcaacc tcaaaaccta acggatgtac acccaggtgc
    22201 ggtgtataac gcactattgc gtgtgaagtc agatcatcgg ggctttgcgg ataaccaaag
    22261 cgcgtcagat agtgcggact ggcacagttg accatggtta gtttgccgag gggacgggcg
    22321 ataacgccat cttccggtaa cgcgccagtg cgtatcacgc aatcaaaacc atcatgaaga
    22381 atatctaccg gacggttatg gctactcagt tccagctcaa ttcccggatg ctgatagaga
    22441 aattccgaca gacgcggcag taacaggttt ttcgcgattc ctgacgggat gtcgatgcgt
    22501 aatttaccgc tgatactggt ggcatcctgt tgaaacagac cgtccagttc gctgagatta
    22561 ctcaacacat cttttgcccg ttgataatag gtcatgcctt ctggcgtgag tttgacccgt
    22621 cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt ccagtgcctg tatctggcgc
    22681 gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc gggaaaaact ttccagctcc
    22741 gcgactttga tgaacaactg cattgcgtga attttatcca tttctttgac cgattgttgt
    22801 tcacagcgaa acagtaacgc gcatatcgca ttgtttattg ttttttctaa catctaatgc
    22861 cgggtttcac aatgttgtca tgacgaatgt ggcgatttct cagcaggaaa agggcggaat
    22921 agctttatga gaaaggtgaa tccccttaaa cagagcgtat ttttcagtga ttccctttaa
    22981 agtgaataga ggacgtattg gctatttgtg cttatttttg tctcattgcc atttgttatt
    23041 ttggcttgta gtcgcggaat ttcattttta attgcatgcg tactaaccac caatataagg
    23101 aaatcctcaa aattgtttta aatagtgccc tcgacatgta atttcaggcg tttagctgta
    23161 actatttata tcggtaacta ttttctacgc gtcatctctg tagaatggcc accgccaaat
    23221 ggcgaattct tattttttct cgcgggtaat acccttccac tacttaagtc tttttaacgt
    23281 attcaggtgg agattcagtt atgcaaatag tcatgtttga caggcagtca atatttattc
    23341 atggaatgaa aatcagttta cagcagcgta ttccaggagt gagtattcag ggggtcagtc
    23401 aggcagacga gttatggcaa aagctggaaa gttaccctga agccttagtt atgctcgatg
    23461 gcgatcagga tggtgagttt tgctactggt tgctgcaaaa aaccgtggtg cagtttcctg
    23521 aggttaaggt gttaatcacg gcgacggatt gcaataaacg gtggttacag gaagttatcc
    23581 attttaatgt gctggccatt gtgcctcgcg attcaaccgt cgagacattt gcgttggcgg
    23641 taaatagtgc ggcgatgggg atgatgtttc tgcctggaga ctggcggacg acgccggaaa
    23701 aggacattaa agatctcaaa tcgttaagcg cccgccagcg ggagatttta accatgttag
    23761 cagcgggcga atcaaataag gagatcggca gagcgctgaa ta
//
LOCUS       DAJTYI010000052        23503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYI010000052 DAJTYI010000000
VERSION     DAJTYI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..678
                     /locus_tag="OO564_002012"
     CDS             <1..678
                     /locus_tag="OO564_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR6653147.1"
                     /translation="EKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEM
                     KLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIR
                     ATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVR
                     EVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAM
                     AWEEHDE"
     gene            908..1918
                     /gene="glpX"
                     /locus_tag="OO564_002013"
     CDS             908..1918
                     /gene="glpX"
                     /locus_tag="OO564_002013"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6653148.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            2015..2761
                     /gene="fpr"
                     /locus_tag="OO564_002014"
     CDS             2015..2761
                     /gene="fpr"
                     /locus_tag="OO564_002014"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6653149.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(2766..3194)
                     /gene="uspD"
                     /locus_tag="OO564_002015"
     CDS             complement(2766..3194)
                     /gene="uspD"
                     /locus_tag="OO564_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6653150.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3221..3520)
                     /locus_tag="OO564_002016"
     CDS             complement(3221..3520)
                     /locus_tag="OO564_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6653151.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(3732..4172)
                     /locus_tag="OO564_002017"
                     /pseudo
     CDS             complement(3732..4172)
                     /locus_tag="OO564_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4273..4872
                     /locus_tag="OO564_002018"
     CDS             4273..4872
                     /locus_tag="OO564_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6653152.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            4980..5747
                     /gene="tpiA"
                     /locus_tag="OO564_002019"
     CDS             4980..5747
                     /gene="tpiA"
                     /locus_tag="OO564_002019"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6653153.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(5802..6557)
                     /gene="cdh"
                     /locus_tag="OO564_002020"
     CDS             complement(5802..6557)
                     /gene="cdh"
                     /locus_tag="OO564_002020"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6653154.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(6664..7653)
                     /gene="sbp"
                     /locus_tag="OO564_002021"
     CDS             complement(6664..7653)
                     /gene="sbp"
                     /locus_tag="OO564_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6653155.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(7973..8935)
                     /gene="pfkA"
                     /locus_tag="OO564_002022"
     CDS             complement(7973..8935)
                     /gene="pfkA"
                     /locus_tag="OO564_002022"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6653156.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9116..10018)
                     /gene="fieF"
                     /locus_tag="OO564_002023"
     CDS             complement(9116..10018)
                     /gene="fieF"
                     /locus_tag="OO564_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6653157.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10166..10666)
                     /gene="cpxP"
                     /locus_tag="OO564_002024"
     CDS             complement(10166..10666)
                     /gene="cpxP"
                     /locus_tag="OO564_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6653158.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            10816..11514
                     /gene="cpxR"
                     /locus_tag="OO564_002025"
     CDS             10816..11514
                     /gene="cpxR"
                     /locus_tag="OO564_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6653159.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            11511..12884
                     /gene="cpxA"
                     /locus_tag="OO564_002026"
     CDS             11511..12884
                     /gene="cpxA"
                     /locus_tag="OO564_002026"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6653160.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(12990..13664)
                     /gene="yiiM"
                     /locus_tag="OO564_002027"
     CDS             complement(12990..13664)
                     /gene="yiiM"
                     /locus_tag="OO564_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6653161.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(13813..14796)
                     /gene="kdgT"
                     /locus_tag="OO564_002028"
     CDS             complement(13813..14796)
                     /gene="kdgT"
                     /locus_tag="OO564_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6653162.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15056..15676)
                     /gene="sodA"
                     /locus_tag="OO564_002029"
     CDS             complement(15056..15676)
                     /gene="sodA"
                     /locus_tag="OO564_002029"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6653163.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            15961..16995
                     /gene="rhaT"
                     /locus_tag="OO564_002030"
                     /pseudo
     CDS             15961..16995
                     /gene="rhaT"
                     /locus_tag="OO564_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(16992..17858)
                     /gene="rhaR"
                     /locus_tag="OO564_002031"
     CDS             complement(16992..17858)
                     /gene="rhaR"
                     /locus_tag="OO564_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6653164.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(17914..18750)
                     /gene="rhaS"
                     /locus_tag="OO564_002032"
     CDS             complement(17914..18750)
                     /gene="rhaS"
                     /locus_tag="OO564_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6653165.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19038..20507
                     /gene="rhaB"
                     /locus_tag="OO564_002033"
     CDS             19038..20507
                     /gene="rhaB"
                     /locus_tag="OO564_002033"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6653166.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            20504..21763
                     /gene="rhaA"
                     /locus_tag="OO564_002034"
     CDS             20504..21763
                     /gene="rhaA"
                     /locus_tag="OO564_002034"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6653167.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22005..22829
                     /gene="rhaD"
                     /locus_tag="OO564_002035"
     CDS             22005..22829
                     /gene="rhaD"
                     /locus_tag="OO564_002035"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6653168.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            22839..23153
                     /gene="rhaM"
                     /locus_tag="OO564_002036"
     CDS             22839..23153
                     /gene="rhaM"
                     /locus_tag="OO564_002036"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6653169.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         5835 a         6149 c         5964 g         5555 t
ORIGIN      
        1 gagaaagcgg tgaaatcaga aaacggcctg ctgaccacca tcgcctgcgg cccgactggc
       61 gaagtgaact atgcgttgga aggtgcggtg tttatggcag gcacatccat tcagtggctg
      121 cgcgatgaga tgaagttgat taacgacgcc tacgattccg aatatttcgc caccaaagtg
      181 caaaacacca atggtgtgta tgtggttccg gcatttaccg ggctgagtgc gccgtactgg
      241 gacccgtatg cgcgcggggc gattttcggt ctgactcgtg gggtgaacgc taaccacatt
      301 atacgcgcga cgctggagtc tattgcttat cagacgcgtg acgtgctgga agcgatgcag
      361 gccgactctg gtatccgtct gcacgccctg cgcgtggatg gtggcgcagt agcaaacaat
      421 ttcctgatgc agttccagtc cgatattctc ggcacccgcg ttgagcgccc ggaagtgcgc
      481 gaagtcaccg cattgggtgc ggcctatctc gcaggcctgg cggttggctt ctggcagaac
      541 ctcgacgagc tgcaagagaa agcggtgatt gagcgcgagt tccgtccagg catcgaaacc
      601 actgagcgta attaccgtta cgcaggctgg aaaaaagcgg tcaaacgcgc gatggcgtgg
      661 gaagaacacg acgagtaatg tcaggcacct gaatcgattg cctgatgctg atcgcctgat
      721 gcgacgctta cgcgtcttat caggcctacg agccaatgcc attttgtgta ggcgggataa
      781 ggcgttcacg ccgcatccgg catcacgcag actctgaaac cgtgcggggg caaccccgca
      841 cacatcaata atcccaccct tcccctgtgc tacacttcgc gccattcctt actgcttaga
      901 gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat ctgcggcgct
      961 ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg cggcggtaaa
     1021 cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca ttggtgaagg
     1081 tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga
     1141 cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga tgggccaggc
     1201 taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg cgccggatat
     1261 gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc tgaacctgcc
     1321 gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga gcgaactgac
     1381 ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc agcaactcgg
     1441 cgtacgcgta tttgctattc cggacggcga cgttgcggcc tcaattctca cctgtatgcc
     1501 agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg tggtttctgc
     1561 ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc gtcatgacgt
     1621 caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac gctgcaaagc
     1681 gatgggcatc gaagccggta aagtattgcg tctgggcgat atggcgcgca gcgataacgt
     1741 catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggtatta gccgcaaagg
     1801 caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca ttcgccgcat
     1861 tcagtccatc cactatctgg atcgcaaaga cccggaaatg caagtgcaca ttctctgagt
     1921 gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt cggagaacga
     1981 agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa caggcaaagt
     2041 cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg cccccgtgct
     2101 tccgtttacc gccgggcaat ttaccaagct tggccttgaa atcgacggcg aacgcgtcca
     2161 gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt acctggtcac
     2221 cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccgggcg atgaagtgca
     2281 ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact gcgaaacgct
     2341 atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc aactaggcaa
     2401 agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg ccgccgactt
     2461 aagctatttg ccactgatgc aagaactgga aaaacgctac gaaggcaaac tgcgcattca
     2521 gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc ggacggatac cggcattaat
     2581 tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa ccagccatgt
     2641 gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga aagagacccg
     2701 gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc attactggta
     2761 agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt attgatcatc
     2821 ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca gacaaggagg
     2881 tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc gcgttcaata
     2941 cgcagttttg tcttcggaca ttgaatattt ttcgtcagtt tatacagctt gttatccgac
     3001 ttattctcca acaattgaag aatatcttct gttgcaggga agtagatacc cggatacaac
     3061 tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg tctggcgagc
     3121 tccagggctt tattcaccag taaggcatct tcttcattcc cggaaattgc cacgccaatg
     3181 tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac gaacgcagtg
     3241 ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca tcaagctcaa
     3301 cttgcccctc cagatcctta atcttatagg ccacgctggc cccttcatct gcagaggatg
     3361 cagcggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg cgggtggcaa
     3421 aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg atatcaatcg
     3481 cacatccttt agtactgcat ttatctacga catctttcat agggcacctc tgtatattcg
     3541 cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca aacaagtaat
     3601 gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca gaaggatttt
     3661 ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag cagtaagaga
     3721 tatattaaag gttaattact tgatttattg tcggctttat acttcacatc ctgagtatct
     3781 ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat aagcatcatc
     3841 accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc cggtaaaatc
     3901 gctcagttac ccgccagcag catccacgcc acaatcatca gaaatgccca tgcgccggaa
     3961 cgcccgcgat catgcaagcg cttaacagtt actgccgctg ttggccagag caagcacaca
     4021 aggcaaaacg ccgcggtctg aatatcgagt agattcttac ccgccagtga gaaaagcacc
     4081 agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc aatacgccct
     4141 ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt gtcttttcca
     4201 ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca gtcataacaa
     4261 aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc tgcattaacc
     4321 gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc ttatctgctg
     4381 gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt taacagccag
     4441 aatcccagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga caaagccaat
     4501 ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc gctggagcgc
     4561 ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg gccagagcaa
     4621 aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac actcacccca
     4681 ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac
     4741 aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa
     4801 aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt
     4861 ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact gtttcacaga
     4921 cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg agaattaaaa
     4981 tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac atggttcacg
     5041 agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg
     5101 caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc cacatcatgc
     5161 tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa acttctgccg
     5221 ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa cgtcgtactt
     5281 accacaaaga gtctgacgaa ctgattgcga aaaaattcgc ggtgctgaaa gagcagggtc
     5341 tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcggga aaaactgaag
     5401 aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg gcattcgaag
     5461 gtgcggttat cgcttatgaa cctgtatggg caatcggtac tggcaaatct gcaactccgg
     5521 ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt gacgctaaca
     5581 tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac
     5641 tgtttgctca gccagacatc gacggcgcgc tggttggcgg tgcttctctg aaagctgacg
     5701 ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc
     5761 cggatttcat atccggcact tactttcctt aactcctcgc cttaacgcaa aatctcacac
     5821 tcatgatcct ggatttcctc ggctgaagca cggttcagcg tcagtagatt tcgttgtgtc
     5881 gccagcaaca caaacgaatt atcactctgc cgtaccatcg ccagcccgta gcttcccata
     5941 tgttcccgcg cctcaggcac ttcttctgca agcatcataa atgggctgcg ttgtaccagt
     6001 tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc tggcagtggc
     6061 aaccagcggc tgctgatgtt cgccagattt ttatccagtt gttcgcgcac atcaggacga
     6121 atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc
     6181 aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa atcacgcgcc
     6241 tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg actttcagta
     6301 ccgttaatac gatacgttga catcaacaga tattgcagtg gaccattaag atcttttaaa
     6361 accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg attttgctgc
     6421 tgattgggca aacattcctc aaggacactc ttacgtaatg tatccgactc ttcaccggtt
     6481 aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac caaaaaaaga
     6541 agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg gtaacgcaaa
     6601 atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat tatcctaggg
     6661 aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc
     6721 tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg gaaacgcatt
     6781 ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg cggcaatttc
     6841 ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt tggtgccttt
     6901 tttctcgacc actttatcga ccaccgacac cgttggctct gcgaggatag actcactcgg
     6961 cgtgacgatt tcgaatttat ctttccccag ttcattcgct gccagcagag cttcgttttc
     7021 ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg agccgcgcgc
     7081 gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct gtgcttttgc
     7141 ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt tccagcgtgc
     7201 gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa tcagatcgtt
     7261 ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg tggaagtgta
     7321 cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc cgcgttccgc
     7381 aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa taccgttgat
     7441 taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca cgttatcacc
     7501 agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt
     7561 tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgctgg ttgccgccag
     7621 caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg ttgtgttgtg
     7681 atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag attggctata
     7741 acttgcgggt atatgttgag ggattaaaaa ggcggaagaa atccgcctca tattgctgac
     7801 aaagtgcgct ttgtccatgc cggatgcggc gcgaacgcct tatccggcct acaaaagttt
     7861 gcaaattcaa taaattgcag aattcatgta ggcctgataa gcgaagcgca tcaggcattt
     7921 ttgcttctgt catcggtttc aggctaaagg aatctgcctt tttccgaaat cattaataca
     7981 gttttttcgc gcagtccagc cagtcacctt tgaacggacg cttcatgttt tcgatagcgt
     8041 cgatgatgtc gtggtgaacc agctgttcgt tctggatacc tacacaacga ccgccgtaac
     8101 ctgccagcag cagatcgata gcgtaagcgc ccatacggga agccagaata cggtcgtaag
     8161 gcaccggaga accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg
     8221 tttctttctc gatgaaatgc gccagttcgt caacatcaca catatgttcg gtaatcgcca
     8281 cgatcgcgtg ttttttacct ttcgcgatac ccgctttgat ttcgtttacc aggtcttcac
     8341 ggctgaattc aacttccgga actacaacga attcacagcc accggcaatg gccgcagcca
     8401 gggtcagatc gccacaataa cggcccatca cttccaccac ggaaatacgc tggtgagaag
     8461 aagaggtgtc acgcagacgg tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac
     8521 cgatagtgta gtcagtgcct ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg
     8581 ggaagcccat ttcggtcaga cgcattgcac ccatgtagga accgtcaccg ccgataacca
     8641 ccagcgcgtc gataccacgt tttttcaggt tttcgatagc cacggcgcgg atgttctcgt
     8701 cgcggaattc cgggaaacgc gcagaaccga ggaacgtacc gccacggttg atcatgtcag
     8761 acacgctgta acggtctagc tgtaccatac ggtcttcata cagacccaga tagccgtcat
     8821 aaatacccat tacttccaga ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg
     8881 cgttcatgcc tggcgcatca ccgccgcttg tcaacacacc tattttctta atcatgacta
     8941 cctctgaact ttggaatgca aaatgaaatc tgttgccgga agtcttcttg cacatcgaag
     9001 tgatccaacg aatgtgcaaa tagtataaca atcacttcct gctgaattga ttcaggtcag
     9061 gccaaatggc ggtattttat acacaaaatg cgggtctggc tctcttttat actgattatg
     9121 aaagcataga ccgtttaccc tccctgggta cgacggaaca ggggtcctga tggataatca
     9181 catccgatcc cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc accatatgtg
     9241 cctgaaccaa aggcagagag tcttccattt ccaaatggat ctgaataaag cgggtcggcc
     9301 ctgactgccg cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat
     9361 caataatttc ttgccgttct tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct
     9421 catatcccat gcgtaacgcg ctatataaaa tatagatgcc gattcccaat gcaaacagag
     9481 catcggcgcg atgccagccg taccaagaca accccagcgc cagcagaatt gcgccgttca
     9541 tcataacatc agactggtaa tgtagcatat cagcccgcac cgcctggctt tgcgtccggc
     9601 gcaccaccca ccgctgaaac gagacaagga taatcgtaca aattagcgcc acaattgtca
     9661 cgataacccc gacgcctgga tctgtcatcg gtgttggaga taccagatgt tgaatacccg
     9721 tcaaaaacag gaatagtgcc gaaccggaga taaacatact ttgcgccagc gccgcgaggg
     9781 actcagcttt accgtgacca aacgagtgat tatcgtcggc aggttgcagg gaatatcgca
     9841 ccaccagtaa attcgtcaac gacgcgccga tatccaccag cgaatccacc agcgcggcga
     9901 gaatactcac cgacccggta taccaccatg caaaaatttt aatcagcaat agcaacgaag
     9961 ccatcgccgt cgcagcaatc gccgcccgac tgaccagccg tccataagat tgattcataa
    10021 atactcccgc tatcaactga cgctagtata acggaagcaa atcatctgca atgcattaag
    10081 cagcaggcaa attgaggata aaaaaaaccc cacatcatgt gggggaagac agggatggtg
    10141 tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct
    10201 tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat
    10261 gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga
    10321 cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc
    10381 gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc
    10441 taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac
    10501 gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa
    10561 gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg cttaatgaac
    10621 tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa
    10681 atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag
    10741 ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc
    10801 ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc
    10861 ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa
    10921 caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt aatgatgccg
    10981 aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt
    11041 atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat
    11101 gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg
    11161 cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa
    11221 gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg
    11281 gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg
    11341 gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc tttcgaccgc
    11401 gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac
    11461 ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca
    11521 gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg
    11581 ttttgatgtt acccaagctc gattcacgcc agatgaccaa gcttctggat agcgaacagc
    11641 gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat ccgcccaacg
    11701 atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc
    11761 gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga
    11821 tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg
    11881 gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt
    11941 atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc
    12001 cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct
    12061 ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa
    12121 acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc gcaggtgcca
    12181 gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctgcttt
    12241 ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac
    12301 tgcgccgtcg tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa gcgcaacgtc
    12361 tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa aacgcgctgg
    12421 ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg
    12481 aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg tggccgctgt
    12541 acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat gctctgcgtt
    12601 attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg
    12661 tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct
    12721 atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg ctggcaattg
    12781 ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc ccgctgggcg
    12841 gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg catttgtagg
    12901 caggataagg cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat
    12961 agcgtcttat caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct
    13021 tcgatcttgc cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg
    13081 gcggcggaga gtaaacggtg atactgatcg tcatcaaacg gcatatgcca tgcgatggcg
    13141 gcagcctcct gcacggtcac atcgctgaca cgggaaacca actccagcgg cgcgtccgca
    13201 gacactttcc ctggcgctat cacgctatac aaccacccca ccttacctgt gttttgcatc
    13261 aactgcgcta tatcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc
    13321 tggctgacct gaattaatgc ctctccccag cggaaaatat cgcccatatg aacattactt
    13381 tccgtgagac cgtcggttga gagattttcg ccaaacgcag gcgcaacaaa caactccgcc
    13441 tgttccggaa attcccgcac ccagtagaga tagtgctcac gaggataatg acacagcgct
    13501 ctgtctggcc cgccgtgaac ctttttctcc gcctgctcgt caccttccag ccccagctcc
    13561 gtcagcatca actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga
    13621 taagcctgaa tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg
    13681 agaaatttcc ccattagacc atatttattt tatgtcagcc gtgctgtttt cgcggtaagt
    13741 atccaggcag gaactcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt
    13801 aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgctgctc tggctttgac
    13861 tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt
    13921 cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat
    13981 cagcacaggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc
    14041 atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattat
    14101 tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt
    14161 attgcccagc gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc
    14221 tcgtaattca gggtcaagat tccccagggc aaagcccacc aggaacggta atactgcgcc
    14281 gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg cccagaataa tcatcgtcat
    14341 gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt tcgtaccgta
    14401 ctgctgcatg atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc
    14461 cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga
    14521 ggcaattgcc gcaaccaccc acgcgacggc aattttagtt accaccagcg taccggattt
    14581 acgcagtacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac
    14641 cgccagaatg ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc
    14701 cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc
    14761 ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt
    14821 taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg aagatagaca
    14881 ttcatttaat caataaaagt gatcaaaacc acatcaattg aaacgctgtt ttatttttat
    14941 aggatcattg tttttatttt ggaattgttc acaaaaaaac caccgccgtt ggcgatggtt
    15001 cattttttaa gctgatatgc ggcctatacg cctcattgca gcaggcggca aatgattatt
    15061 ttttcgccgc aaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttga
    15121 tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc
    15181 ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcgga gaatcctggt
    15241 tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac
    15301 caaagcggga agctgccgct ttttcaaatt ctgctttgaa gttatcaacg gagccgaagt
    15361 cacgttcgat agccgctttc aggtcaccct gcagggtggt gccttttttc agacctttcc
    15421 agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg
    15481 ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca aattctggca
    15541 ggctttccag cgccgcgttg gcgttgttta cgtaggtctg atggtgtttg gtgtggtgga
    15601 tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg
    15661 gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt
    15721 aagcagttgg tttattatag ttaattaaat gatattgaaa atgattatca atgccgcact
    15781 tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg tgaagcaaat cacccactta
    15841 atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg cgaaaggtta atcgacagca
    15901 cgatttttac actcatctcg tcggagatgt gaagcgacga aaaatgatga ggataagaag
    15961 atgagtaacg cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc
    16021 tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtc
    16081 ggtgggattg tttcgtggat tattctgccg tgggccatca gcgccctgtt actaccggat
    16141 ttctgggcgt attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc
    16201 gctatgtggg ggatcggtaa tatcaactat ggcctgacca tgcgttatct cggcatgtcg
    16261 atgggaattg gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt
    16321 atcaacggca atttcgatgt gctgattaac accgaaggcg gacgcatgac gttactcggc
    16381 gttctggtgg cgctgattgg cgtagggatt gtaactcgag ccgggcagtt gaaagagcgc
    16441 aagatgggca ttaaagccga agagttcaat ctgaaaaaag ggctggtgct ggcggtgatg
    16501 tgcggcattt tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa
    16561 gccgctgccg cacttggcgt cgatccactg tatgtcgctc tgccaagcta tgttgtcatc
    16621 atgggcggcg gcgcgatcat taacctcggt ttctgtttta ttcgtctggc aaaagtgaag
    16681 gatttgtcgc taaaagccga cttctcgctg gcaaaaccgc tgatcattca caatgtgtta
    16741 ctctcggcac tgggcgggtt gatgtggtat ctgcaattct ttttctatgc ctagggccac
    16801 gcccgcattc cggcgcagta tgactacatc agttggatgc tgcatatgag tttctatgta
    16861 ttgtgcggcg gtatcgtcgg gctggtgctg aaagagtgga acaatacagg acgccgtccg
    16921 gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg ccgctaacat cgtcggcatc
    16981 ggcatggcga attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg
    17041 gtttcccggg taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggtcgaa
    17101 atatcactga ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc
    17161 tgccgcagat attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg
    17221 cgctcactgc acgatgcctc atcacaaaat ttatccagcg caaagggact tttcaggcta
    17281 gccgccagcc gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa
    17341 tcactggtgt aacgatggcg attcagcaac atcaccaact gcccgaacag caactcagcc
    17401 atttcgttag caaacggcac atgctgacta ctttcatgct caagctgacc gataacctgc
    17461 cgcgcctgcg ccatccccac gctacctaag cgccagtgtg gttgccctgc gctggcgcta
    17521 aatcccggaa tcgctccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata
    17581 atattctgca aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaata
    17641 taaaagagat cgccacgggt aatgcgataa ggacgatcgt tgagtacatg cagaccatta
    17701 ccgcgccaga caatcaccag ctcacaaaaa tcatgtgtat gttcggcaaa gacatcttgc
    17761 ggataacggt cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga
    17821 agttttaact gatgcgccac cgtggctacc tcggccagag aacgaagttg attattcgca
    17881 atatggcgta caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg
    17941 gcgaatatca cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact
    18001 gtcgctgaat ccacagcgat aggcgatgtc agtgacgctg gcctcgctgt ggcgtagcag
    18061 atgtcgggct ttcatcagtc gcaggcggtt caggtatcgc tgaggcgtca gtcccgtttg
    18121 ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa aattgatccg ccacggcatc
    18181 ccaattcacc tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga
    18241 tgcgctgttt tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat
    18301 aaacaagatc tcgcgactgg cggtcgaggg taaatcattt tccccttcct gctgttccat
    18361 ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg ttaacgcgcc agtgagacgg
    18421 atactgccca tccagctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg
    18481 atccggcgag cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg
    18541 ccgatcatga tcgcgtacga aacagaccgt gccaccggtg atggtatagg gctgcccatt
    18601 aaacacatga atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg
    18661 ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt tacaagacgg
    18721 aaaaaaatcc acactacgta atacggtcat actggcctcc tgatgtcgtc aacacggcga
    18781 aatagtaatc acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa
    18841 cgtcgatttt tcaagataca gcgtgaattt tcaggaaatg cggtgagcat cacatcacca
    18901 caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt
    18961 ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa
    19021 ttcagcagga tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca
    19081 gtgggcgcgt gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa
    19141 tccatcgttt taacaatggg ctgcatagcc aaaacggtta tgtcacctgg gatgtggata
    19201 gcctggaaag tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg
    19261 atagcattgg gattgatacc tggggcgtgg actttgtgct gctcgaccaa cagggtcagc
    19321 gtgtgggcct gcccgttgct tatcgcgata gccgcaccaa tggcctgatg ccgcaggcac
    19381 aacagcaact cggcaaacgc gatatttatc agcgtagcgg catccagttt ctacccttca
    19441 atacgcttta tcagttgcgt gcgctgacgg agcaacaacc tgaacttatt ccacacattg
    19501 ctcacgctct gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg
    19561 aatataccaa tgccaccacc acacaactgg tcaatatcaa tagcgacgac tgggacgagt
    19621 cgctactggc gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta
    19681 atgtcattgg gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca
    19741 gccatgatac cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc
    19801 tctcttctgg cacctggtca ttgatgggct tcgaaagcca gacgccattt accaatgaca
    19861 cggcgctggc agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga
    19921 aaaatattat gggcttatgg ctgcttcagc gagtgcttca ggaacggcaa atcaacgatc
    19981 tcccggcgct tatcgccgcg acacaggcac ttccggcctg ccgcttcatc atcaatccca
    20041 atgacgatcg ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg
    20101 aaacggcgca accgatcccg ggaagtgatg ctgaactggc gcgctgtatt ttcgacagtc
    20161 tggcgctgct gtatgccgat gtgttgcatg agctggcgca gctgcgcggt gaagatttct
    20221 cgcaactgca tattgtcggc ggcggctgcc agaacacgct gctcaaccag ctatgcgccg
    20281 atgcctgcgg tattcgggtg atcgccgggc ctgttgaagc ctcaacgctc ggcaatatcg
    20341 gcatcgagtt aattacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca
    20401 gcaccaccgc gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg
    20461 tggcgcagat tcactctaca cgacagacaa aggagctttg cgcatgacca ctcaactgga
    20521 acaggcctgg gagctggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc
    20581 gctgcgccaa cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc
    20641 cggttttgaa aacccggaag gttcgctgac cggggggatt caggccacag gcaattatcc
    20701 gggcaaagcg cgtaatgcca gtgagctacg tgccgatctg gaacaggcta tgcggctgat
    20761 tccggggccg aaacggctta atttacatgc catctatctg gaatcagata cgccagtctc
    20821 gcgcgaccag atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca
    20881 gctcggtctg gattttaacc cctcctgctt ttcgcatccg ctaagcgccg atggctttac
    20941 gctctcgcat cccgacgaca gcattcgcca gttctggatt gatcactgca aagccagccg
    21001 tcgcgtttcg gcctattttg gcgagcaact cggcacacca tcggtgatga acatctggat
    21061 cccggatggt atgaaagata tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc
    21121 agcactggat gaggtgatca gcgagaagct aaaccctgcg caccatatcg acgccgttga
    21181 gagcaaattg tttggcattg gcgcagagag ctacacggtt ggctccaatg agttttacat
    21241 ggggtatgcc accagccgcc agactgcgct gtgcctggac gccgggcact tccacccgac
    21301 tgaagtgatt tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca
    21361 cgtcagccgt ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca
    21421 ggcaattgcc agtgagattg tgcgtcacga tctgtttgac cgggtgcata tcggccttga
    21481 cttcttcgat gcctctatca accgcattgc cgcgtgggtc attggtacac gcaatatgaa
    21541 aaaagccctg ctgcgtgcgt tgctggaacc taccgctgag ctgcgcaagc tggaagcggc
    21601 gggcgattac actgcgcgtc tggcactgct ggaagagcag aaatcgttgc cgtggcaggc
    21661 ggtctgggaa atgtattgcc aacgtcacga tacgccagca ggtagcgaat ggctggagag
    21721 cgtgcgggct tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc
    21781 acaagcgcct tatccggcct acgggtcgaa tgtccatgcg ggctgtcacc ctcaccccgg
    21841 ccctctccct gaaagggcga gggagaaaaa catagcctcg tgcggcaacg gagctgaaca
    21901 ccgctccgga cagtcccctc gcccctacgg ggagagggtt agggtgaggg gaacaagccg
    21961 caccatgtct aaacaaggca acctaattta caggaacaca gaacatgcaa aacattaccc
    22021 agtcctggtt tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg
    22081 acgagcgcaa cggcggcaac ctgacgctgc gcctggatga cgccgatatc gcgctttatc
    22141 acgacaactt ccacccacaa ccgcgctata ttccgctcag ccaacccatg cctttactgg
    22201 caaatacgcc gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagctcgatc
    22261 ctgcagctaa cttaggcgtc gtaaaagtcg acagcgatgg cgcgggctac catattctct
    22321 gggggttaac caacgaagcc gttccaactt ctgaacttcc ggctcacttt ctctcccact
    22381 gcgagcgcat taaagccacc aacggtaaag atcgggtgat catgcactgc cacgccacca
    22441 acctgatcgc cctcacctat gtactggaaa acgacaccgc ggtctttact cgccaactgt
    22501 gggaaggcag caccgaatgt ctggcagtat tcccggatgg cgttggcatt ctgccgtgga
    22561 tggtgcccgg cacggacgaa atcggccagg cgaccgcaca agagatgcaa aaacattcgc
    22621 tggtgttgtg gcccttccac ggtgtgttcg gttccggacc gacgctggat gaagcctttg
    22681 gtttaatcga caccgcagaa aaatcagcac aagtattagt gaaggtttat tcgatgggcg
    22741 gcatgaaaca gaccatcagc cgtgaagagt tgatagcgct cggcaagcgc ttcggcgtta
    22801 caccactagc cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca
    22861 tgcaggtaaa ccccgacgcc cacgaagagt atcagcgtcg gcataatccc atctggccgg
    22921 aactggaagc agtgttgaaa tctcacggtg tgcataacta cgccatttac ctcgacaaag
    22981 cgcgtaatct gctgtttgcc atggtagaga ttgaatctga agaacggtgg aatgcggtag
    23041 ccagtactga tgtttgccaa cgttggtgga aatatatgac cgatgttatg cccgctaacc
    23101 cagataacag cccggtgagt agcgagctgc aagaagtgtt ttacctgccg taattgccca
    23161 atgtgccgga gatgcctccg gcacaatatt ttaaatttcg atttgaaaaa ttaaacatat
    23221 tacatctccc ggtaagttat atttccctga tacattgtga gtaaatcaca aaaataatga
    23281 ataacccatt aatgattcat gtggtttatt taaataacca ttatgtgcat tactccgcaa
    23341 atctgacctt tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac
    23401 ttaatcgctc caccattaca attgattaaa atatatttta tggagtccga ttaatggcag
    23461 ctcttactgc aagctgtatt gacctgaata ttcagggcaa tgg
//
LOCUS       DAJTYI010000053        23289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYI010000053 DAJTYI010000000
VERSION     DAJTYI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..183
                     /locus_tag="OO564_002037"
     CDS             <1..183
                     /locus_tag="OO564_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6653170.1"
                     /translation="FKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGG
                     IEPALKALNELEHQTK"
     gene            236..1966
                     /locus_tag="OO564_002038"
     CDS             236..1966
                     /locus_tag="OO564_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653171.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2026..2517)
                     /gene="yajQ"
                     /locus_tag="OO564_002039"
     CDS             complement(2026..2517)
                     /gene="yajQ"
                     /locus_tag="OO564_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6653172.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2685..3596
                     /gene="panE"
                     /locus_tag="OO564_002040"
     CDS             2685..3596
                     /gene="panE"
                     /locus_tag="OO564_002040"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6653173.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3559..4149
                     /gene="yajL"
                     /locus_tag="OO564_002041"
     CDS             3559..4149
                     /gene="yajL"
                     /locus_tag="OO564_002041"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6653174.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTTSVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4203..5651)
                     /gene="thiI"
                     /locus_tag="OO564_002042"
     CDS             complement(4203..5651)
                     /gene="thiI"
                     /locus_tag="OO564_002042"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6653175.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5857..6099
                     /gene="xseB"
                     /locus_tag="OO564_002043"
     CDS             5857..6099
                     /gene="xseB"
                     /locus_tag="OO564_002043"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6653176.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6099..6998
                     /gene="ispA"
                     /locus_tag="OO564_002044"
     CDS             6099..6998
                     /gene="ispA"
                     /locus_tag="OO564_002044"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6653177.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7023..8885
                     /gene="dxs"
                     /locus_tag="OO564_002045"
     CDS             7023..8885
                     /gene="dxs"
                     /locus_tag="OO564_002045"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6653178.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8940..9914
                     /gene="yajO"
                     /locus_tag="OO564_002046"
     CDS             8940..9914
                     /gene="yajO"
                     /locus_tag="OO564_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6653179.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLKALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9968..10486)
                     /gene="pgpA"
                     /locus_tag="OO564_002047"
     CDS             complement(9968..10486)
                     /gene="pgpA"
                     /locus_tag="OO564_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6653180.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10464..11441)
                     /gene="thiL"
                     /locus_tag="OO564_002048"
     CDS             complement(10464..11441)
                     /gene="thiL"
                     /locus_tag="OO564_002048"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6653181.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11519..11938)
                     /gene="nusB"
                     /locus_tag="OO564_002049"
     CDS             complement(11519..11938)
                     /gene="nusB"
                     /locus_tag="OO564_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6653182.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11958..12428)
                     /gene="ribE"
                     /locus_tag="OO564_002050"
     CDS             complement(11958..12428)
                     /gene="ribE"
                     /locus_tag="OO564_002050"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6653183.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12517..13620)
                     /gene="ribD"
                     /locus_tag="OO564_002051"
     CDS             complement(12517..13620)
                     /gene="ribD"
                     /locus_tag="OO564_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6653184.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13624..14073)
                     /gene="nrdR"
                     /locus_tag="OO564_002052"
     CDS             complement(13624..14073)
                     /gene="nrdR"
                     /locus_tag="OO564_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6653185.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14224..14763
                     /locus_tag="OO564_002053"
     CDS             14224..14763
                     /locus_tag="OO564_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6653186.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15062..15946
                     /gene="tsx"
                     /locus_tag="OO564_002054"
     CDS             15062..15946
                     /gene="tsx"
                     /locus_tag="OO564_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6653187.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16123..16470)
                     /locus_tag="OO564_002055"
     CDS             complement(16123..16470)
                     /locus_tag="OO564_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6653188.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16599..17570)
                     /gene="secF"
                     /locus_tag="OO564_002056"
     CDS             complement(16599..17570)
                     /gene="secF"
                     /locus_tag="OO564_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6653189.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17581..19428)
                     /gene="secD"
                     /locus_tag="OO564_002057"
     CDS             complement(17581..19428)
                     /gene="secD"
                     /locus_tag="OO564_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6653190.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19456..19788)
                     /gene="yajC"
                     /locus_tag="OO564_002058"
     CDS             complement(19456..19788)
                     /gene="yajC"
                     /locus_tag="OO564_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6653191.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19811..20938)
                     /gene="tgt"
                     /locus_tag="OO564_002059"
     CDS             complement(19811..20938)
                     /gene="tgt"
                     /locus_tag="OO564_002059"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6653192.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20993..22063)
                     /gene="queA"
                     /locus_tag="OO564_002060"
     CDS             complement(20993..22063)
                     /gene="queA"
                     /locus_tag="OO564_002060"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6653193.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22156..22729
                     /gene="acpH"
                     /locus_tag="OO564_002061"
                     /pseudo
     CDS             22156..22729
                     /gene="acpH"
                     /locus_tag="OO564_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22734..>23289)
                     /locus_tag="OO564_002062"
     CDS             complement(22734..>23289)
                     /locus_tag="OO564_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6653194.1"
                     /translation="CMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLP
                     LAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRK
                     KSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQC
                     KEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5923 a         6083 c         5733 g         5550 t
ORIGIN      
        1 ttcaaaaaag cagctgcaca aggtaatttt gccgcacatt tctatctggg aaggatgtat
       61 cagaatggtg acggcgttga gagaaatatg gaaaaagcca ggtttcatct atctaatgcg
      121 gcagaaggtg gaatagaacc tgcgcttaaa gcattaaatg aacttgaaca tcaaacaaaa
      181 taaaacgctc agggaccacg taaggttcat caaacagaaa aggatatagt ttgcgatgaa
      241 agcgaaatct actttgttat taatgatttt atctgttttt tattctcatc aggttttatc
      301 cagcgacccc actcagccgg atgtttattc tgtcgtcgag aaaaaactaa actctgcttt
      361 acctctggaa gaaaatacca ccttcagatc gcaggctgaa tggtttaatt tgcaatatga
      421 attattagta tctggctacc ctgaacgtgc ttacaattta ctgagcaaac ttgagacaga
      481 atcgaaaaac gctaaatatt atctggattt aactgcctcc agagtcgctg aagaagaagc
      541 accagagaca gccttaactt ttttatcaaa aattgggtta aataaaccgg ctactcttac
      601 atcctttgag agctatatca atagctggat aaaaagcaaa caacccgaaa aggctatgca
      661 gcttctcagc ctcgatgaga atgccctgaa ttattattta ccgactgtgt tagtcgcata
      721 tcagatcgtt cctgataagg caataagtat ttataattct gtatatggag ataatgttgt
      781 tataccctat cagcaagtca ccatgttact tacagttgcg gagggttatc aaacaaaagg
      841 cgatataaaa aacgccattc attatgccga taaggcgctg aatatgtttg ataaagctat
      901 tgctgtagat agcagtcgtg aatcccatta tagtgatgag tatttgaaac tcatgaatat
      961 ctacgccgcc aatggcaaca aagaaaaagc ggtggtttta tctcaacgtc tacacaaagc
     1021 cattaccgaa tctcaatcga attacttaag tactctggaa ggaattttat tgttttatag
     1081 aaataatgat atgcagcaag actatcaaaa cagcctttca aattacatag tatttatcga
     1141 tgaaattttt gccttttccc catcaacaag aagtgaatta agcctcatta atttattaaa
     1201 ttcattaaat gaggtggaat tgatgagaaa acgtctggcg tttctgatga cagcaccaga
     1261 atacgcttgt tatgatagtc agtattgtta tgaagacaaa gtcagcgcat taaaaatcct
     1321 gtatcgtcat catgacaatg agctaatgga caaatatttc acgactcttc ttaaagagat
     1381 cagtaatcaa ccattagaag actgggatat aataataagt aatactggtg aaaaatttag
     1441 tgaagtgggt ctaaccaaat atgctcaaca tctcgcatct ttcgctgaag aattttatat
     1501 aaaagagcaa aaatcacatt ctgaaagtga aatgagagga ctattttcat cacttgccga
     1561 attatatagt tttggtaatg atactgccag cgcaaaccga gtgttccatc agcatgtccc
     1621 tgccttcaat acagatcata tgattcaata ttttataaat gccaaagaat ggaacaatgc
     1681 cagggaatta ttgataaaag aggaaatgtt gggtatgcat aatctgatat tgttggagaa
     1741 tatttgtatg caaaaaaatg cagaatgcat ggcacacatt acctttacgc ttaataaatt
     1801 aacaacacaa ccagcaataa caaaaataga tagcgtgggt aatgagcaac tttatcagat
     1861 tggcaaaatc taccataagt tggaaattaa acctgagcca gagcaacagg ttttaattca
     1921 gtcgctttat gaccgggcat caggaagcgc ctctgcaact caataaatgc atggtcaaaa
     1981 gggccaccac ggtggccctg acaatcagta gctatatcca gaaagttaat cgcggaagtt
     2041 tttgaactgg aacggctgac cgagatcgcc accgcgcacc atcgccatga cagcctgcaa
     2101 atcatcacga gatttgccag tcacgcggat ctcatcgccc tgaatttgcg cctgcacttt
     2161 cagtttgctg tctttgatca tcttgacgat ttttttctga gtggcgctct caatgccctg
     2221 tttcagtttc gcttccacaa accaggtttt accgctatga acgatatttt ccggtacatc
     2281 cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     2341 attgacctgg aagtcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     2401 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcgac tcgcgttatc
     2461 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     2521 ttcctctccc ttcatttttg atgcgaagca taatacctgc aaagttaaat aaccaacagt
     2581 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     2641 tagccttatt gaacacccgg agtggttgtg ggtgaggagg aacaatgaaa attaccgtat
     2701 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgtaaacag ggtcatgaag
     2761 ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt gaatctggtt gagacagatg
     2821 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc accagcgatc
     2881 tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcatcca
     2941 cactgcctgt aactacgcca atactgttaa ttcacaacgg catgggcacc atcgaagaat
     3001 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     3061 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat cggtccggca cggcaacagg
     3121 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     3181 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgcgtg attaatccac
     3241 tgactgccat ctggaattgc ccgaacggtg aattacgtca tcatccgcaa gaaattatgc
     3301 aaatatgcga agaagtcgcg gcagtgatcg aacgcgaagg gcatcatact tcagcggaag
     3361 atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt
     3421 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     3481 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     3541 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     3601 gagactgaag ccgtcaccac gatcgacctc ttggttcgcg gcggtatcaa agtcaccact
     3661 accagcgtcg acagcgatgg caacctggca attacctgct cgcgcggcgt gaagctgctg
     3721 gcggatgcgc cgctggtcga agtggctgat ggcgaatatg acgtgatcgt gctgcctggt
     3781 ggcattaaag gcacggagtg ttttcgcgat agcactctgc tggttgaaac cgttaaacag
     3841 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     3901 ccgcacgata tctttccgat tggcaatatg accggcttcc cgacgctgaa agacaaaatt
     3961 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     4021 agccaggggc cgggtacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     4081 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     4141 tacgagtagt gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt aaaaactaca
     4201 aattacgggc gatatacctt cacattgtta aagccttgct cgcgcagata gagcgcctgc
     4261 agacggctca tcaccccgcg ctcacaccac agcagccagg ttttgttctg gtcgagatcg
     4321 ccaaatttgg tgctcagttt atagaacggc agagaaacca catcaatccc ttcgactttc
     4381 agtggcttat cttcctgttc atcgacagaa cggatatcga gaatcacgtc gttcgggccg
     4441 aagccgttga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     4501 atatcaacgt tattcgcttc ctcaaccact ttatcgagaa tactgaagtc gaacttctct
     4561 tcttccgctt caatcttcga tttaaccgct ttcaccgtcg ggcttttgga gatcacgcca
     4621 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     4681 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtgtcaga gacgttatca
     4741 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4801 agcgcctgta cgccgtaacg ttcagccact ttagatgcgg cacgcaccat catacgtttg
     4861 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac aaccggttcg
     4921 aaattaatgg cgacaaaacg cacgcggtgg gagctgccaa agcggttcca cagataatgc
     4981 gccacctgac gcacgccaat ttcatgcgct gcgccgccga ggttaaagaa gcagtaatgc
     5041 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     5101 atgagcgaca gcacatcttc ctgggtgcca atcgggaaac caccaatacc ttcgtagcga
     5161 cctttaatca gcaggaggcg atcgtcttcc acttccagat gaaccgttac atccgggtta
     5221 gtcagcttca cgcgcgcgga ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     5281 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     5341 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     5401 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     5461 cgaatagtca gacgctggtt ttcatctttc gcgcgaactt cgatgttatc ccagtggcgg
     5521 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     5581 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     5641 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     5701 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     5761 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5821 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggcgccc
     5881 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5941 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     6001 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     6061 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaattcg
     6121 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     6181 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc
     6241 gacctttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac acgctggacg
     6301 cacccgctgc tgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     6361 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     6421 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     6481 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     6541 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     6601 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     6661 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     6721 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6781 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6841 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6901 acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc actcgatacc tcggcactgg
     6961 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     7021 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     7081 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     7141 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     7201 ccgtggcgct gcactatgtc tacaacaccc catttgacca attgatttgg gatgtgggac
     7261 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     7321 agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     7381 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     7441 aaggcaaaaa tcgccgcacc gtctgtgtga ttggcgacgg cgcgattacc gctggcatgg
     7501 cgtttgaagc gatgaatcac gcgggcgata ttcgtcctga tatgctggtg gtcctcaacg
     7561 acaatgaaat gtcgatttcc gaaaatgttg gcgcgcttaa caaccatctg gctcagctgc
     7621 tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgtgc
     7681 cgcccattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtggtgcctg
     7741 gcacgttgtt tgaagagctg ggatttaact acatcggccc ggtggacggt cacgatgtgc
     7801 tggggcttat caccacgcta aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7861 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agacccgatc actttccacg
     7921 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7981 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     8041 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     8101 cggatcgtta cttcgacgtg gctattgccg agcaacacgc agtgaccttt gccgccggtc
     8161 tggcgattgg tggatacaaa cccattgtcg cgatctactc caccttcctg caacgcgcct
     8221 atgatcaggt gctgcatgac gtggcaattc aaaagctacc ggtcctgttc gccattgacc
     8281 gcgcgggcat tgttggtgct gacggtcaaa ctcaccaggg cgcttttgat ctctcttacc
     8341 tgcgctgcat accagaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     8401 tgctctatac cggctatcac tataacgatg gcccgtccgc ggtgcgctac ccgcgcggca
     8461 acgcggttgg cgtggaactg acgccgctgg aaaaactacc aattggcaaa ggcattgtga
     8521 agcgtcgtgg cgagaaactg gtgatcctta actttggtac gctgatgcca gaagcggcca
     8581 aagtcgccga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccacttgatg
     8641 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     8701 ccattatggg cggcgcaggt agcggagtga acgaagtgct gatggcccat cgtaaaccag
     8761 tacccgtgct gaacattggc ctgccggact tctttattcc gcaaggaact caggaagaaa
     8821 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggctgg
     8881 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     8941 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     9001 tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa gagaacagcc
     9061 gtcccataat taaacgcgcg ctggaaggtg gcataaattt ctttgatacc gccaatagct
     9121 attccgatgg tagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     9181 aagacgtagt cgttgccacc aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     9241 cccgtgcaca aattttgcgc tctatcaacg acagcctgcg acgtctcggc atggattatg
     9301 tcgatatcct gcaaattcat cgcggggatt acaacacgcc gatcgaagag acgctgaaag
     9361 ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg
     9421 cttcgcagtt tgctcaggcg ctggaactac aaaaacagca ctgctgggcg cagtttgtca
     9481 gtatgcagga tcactataat ctgatttatc gcgaagaaga gcgcgagatg ctgcctctgt
     9541 gttatcagga gggcgtggcg gtgattccgt ggagcccgat ggcgcggggg cgactgacgc
     9601 gtccatgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata
     9661 aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac
     9721 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt aagtaaaccg ggcattgccg
     9781 caccgattat cggaacttcg cgggaagaac agcttgatga gctgctgaac gcggtggata
     9841 tcactttgaa gccagaacag attgccgagc tggaaacgcc gtataaaccg catgcggtcg
     9901 taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat caggcatcgg
     9961 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatgcctg
    10021 cggaaatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca tgtacattgc
    10081 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atcacaaacc
    10141 cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc cacataccaa
    10201 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc gccgtttgat
    10261 gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag agctgccagg
    10321 gtaaaaaggt catcagatac cagaaaggga tcgccgccag cgatcccatc gtgccaggaa
    10381 cgattgggct taatccactt ccgaatccga cagcaagcag atgccacgga ttactcatct
    10441 tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc tttccagtca
    10501 aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc ggtcatttgc
    10561 ccgatacagg taaacggtac gccaaggtgt ccgagagcaa catccagcgc gccacggttc
    10621 agttccggca ccgtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
    10681 gcctgttccg gttcaacatg gcgagaaagc acatcagaaa acggcagcaa tgccaggtca
    10741 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
    10801 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gcccctgtaa aatacgcggc
    10861 gatggacgga gatgacgttt gatcaagtag tcagcatctt tagcatcggc aacctgcaaa
    10921 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
    10981 cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
    11041 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
    11101 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
    11161 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
    11221 cttaggttca ccgccagtgc tttataagcc agatcagcag gatcgatatc agggaggaaa
    11281 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
    11341 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    11401 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    11461 aaaaaagaaa aggccggcga tcatggaaca gtcttccgtg aatctaccgg cctggatatt
    11521 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    11581 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    11641 atggcacatc actacgttta gacagttcgt acagcgcaat gcgcagtact gctttttcta
    11701 cctggcccag ttcttccagc aggcgggaca ggtagggctt catcagtccg tcgaggtatg
    11761 cggtgttagt cgccaccccg gccagcagct cacggaagta cagaacgtca acgtctttta
    11821 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    11881 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    11941 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    12001 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    12061 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    12121 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcaga gccaccacga
    12181 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    12241 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    12301 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    12361 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    12421 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    12481 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcccccacta aatgcaggca
    12541 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    12601 ctcaagccct ggcagcgagc ataatccacg ggcgtcgctg cctaatagtt taggtgcgat
    12661 atagacaatc agttcatcga ctaaacccgc ctgtagcaat acgccagcga gcgttggccc
    12721 cgcttccacc cagatgctgt taatctgctg tttacccagt tgcatcatca gtacaaccag
    12781 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcacg
    12841 agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc
    12901 cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga gattttgttg
    12961 cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta aggctggatc
    13021 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg
    13081 tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    13141 gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    13201 cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt ggctgacgtc
    13261 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    13321 catcgctgcg accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    13381 acggccatga tggctacagg gttcgagtgt cacatacgcc gttgcgcctt tggctttttc
    13441 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgctcgtt ggtggtaacc
    13501 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tggggtgggt
    13561 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    13621 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    13681 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    13741 cagattgcca atcatcttgc tcggtacttc gcgctcaccg gtggcgcgca gctgcgattt
    13801 aatatgattg attgccattt caacgtaatc ggaactcacc ggacgttttt ccagcgcccg
    13861 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    13921 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    13981 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    14041 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacgg
    14101 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    14161 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    14221 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    14281 tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg tcagcacgct gaaccaggag
    14341 atgacgcaac tcaatcaaga aacagtcaaa atcactcagc aaaacaggct gaatgcaaaa
    14401 tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact ggaaagccag
    14461 atcggtactt tacgtatgtc gctggtgaat attacgcctg atacagatgg caccaccctt
    14521 acactacgta ttcagggtga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    14581 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    14641 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acaattaaag
    14701 gggatttctg tggatcagtt aggctttgta cgtatccacg acattcaacc tgttatgcag
    14761 taaacgtatt ttgggacgat tttgtgcgtc ccgcaacatc tttccccgtc attttgttac
    14821 tctgcttaca tcacttggat tgatagtaaa agtttgcaac aagggcgaaa gtcagtacaa
    14881 tccccgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat agaactgtga
    14941 aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta tacgaaatat
    15001 ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca gtggcataca
    15061 tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt cttttactgt
    15121 caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc agagcgttaa
    15181 cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata cctaccttga
    15241 gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg atgcgccggt
    15301 attcttcggc ggtaactccg atgctaaagg tatctggaac cacggttctc cgctgtttat
    15361 ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta gcttcggtcc
    15421 gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta ataaagatgg
    15481 tcgccagagc acctggtaca tgggtctggg taccgatatc gacactggcc tgccgatgag
    15541 cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag cgaacgaaaa
    15601 cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg atctgtgggg
    15661 cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt taggggatga
    15721 cagcggtaac gcaatcaacg gtattaagac ccgtactaat aactctatcg cttccagcca
    15781 tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt actggcacga
    15841 cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact tcaacgttcg
    15901 ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat gaaaatgccg
    15961 ggatttattc ccggcatttc tgattgttaa caacatgtgc tttgttcatg ccggatgcgg
    16021 cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca atatattgct gaaataacgt
    16081 aggcctgata agcgtagcgc atcaggcaat tttacgtttt aatcacttct tcttgttcat
    16141 catcgctttc agatcagcga atgggttgta cttcgcttca ccgacatctt tctgcgcatc
    16201 ttcccctgcg ataacggtcg taccatactg atccgcttcg gtatatttcg aatgctcatg
    16261 atcgtggcaa tagagacaca acaattccca gttactgcca tcttccgggt tattggtatg
    16321 gtcgtgatca atgtggtgaa cggtaagttc acgcaggtta gaataaacaa actcgcggga
    16381 acagcgaccg cagacccacg gatagatttt taatgctttt tcgcgatagc cgctttctaa
    16441 ccgtgcatag ttttttggga tgatagccat gaaaagttac ctggatgaaa tgtagggtta
    16501 cgcgcagttt cccaagatgc ggggattttc gcaatctcca tacatcaaaa aaaatcccga
    16561 tcttctgacc gggattttca acatcaacgg gaacttgatt aaggcagaat tgacggctga
    16621 tccgcccctt ctttttccac tttctgctgc aacatgtgtt cgcgcttcat acccagtttc
    16681 agagccaacg cagatgctac gtagatggaa gatgcagtgc cgatggaaac accgataagc
    16741 atggtcagcg agaagccttc cagtaccgga ccaccgaaga gatacagcat caggataacc
    16801 atcaaggtag taccggatgt gatcaaggta cggtgcagcg tctgggtcaa ggacacgtta
    16861 aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt tttcacgaat tcggtccgat
    16921 accacgatac tgtcgttaag cgagtaaccg ataaccgaca tcaacgatgc cacaatggtc
    16981 aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac gtcgtgcgcc
    17041 agcgcaataa ccacccctgc cgccagtcgc cactcaaagc ggaaacctac gtacacgagg
    17101 atagacagca gcgctgccat caacgccatc gcaccggttt gcgcaaggtc tgcccccacg
    17161 ctcggaccga cgaactcaat acgcttcact gctgcattct gattggtgga ttcgttaatc
    17221 accttcagaa cttggctgcc cagcacctga ccgccggttt cgccttcagc aggcggcata
    17281 cggaccatga tgtcgtggct gctaccaaag ttttgcagca ttggctcttc aaaaccggct
    17341 ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt aatttcgata
    17401 accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat aataacgata
    17461 gcagcgatta acagcagacc agagatgccg aaagcccagt agtcccagcg cataaagtca
    17521 tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac atcgcactcc
    17581 tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga tggcacgcgt
    17641 accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa tcgcgaaccc
    17701 tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg tggtgatgtt
    17761 cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct gttgaacagt
    17821 acgtccgttg ctcaactctt ctttaatacg ttcgttgatc agtacgttcg catcgaccgc
    17881 taccgcaagg gttaagacga tacccgcgat acctggcata ctcagtgttg cgcctggcaa
    17941 cagcgacata ataccgacga tcaagatcaa gttggcaatc agagcactgg tcgcaatcag
    18001 accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac cggcaaggca
    18061 agcctccagc ccctgctcaa tgttctgcat acccagggtc gggccaattg tacgttcttc
    18121 aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca gctgacgggc
    18181 ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta cccagacgag actggatgtt
    18241 ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccatttg catctttctt
    18301 accgctgtct ttgtactcca caaacagggt tgccatcggt ttgccgatgt tgtccttggt
    18361 gaagttagac ataatgttgc caccagcgct atcgagcgag atgttaacct gcggctggtt
    18421 gtattcgtcc tggctggaag tggagtcggt gatatggtca ccagtcagaa ttacgcgttt
    18481 gtacagcaca accggctgac cttcgcgggt ctgtttcact tcagagtcgc ccggtacgcg
    18541 accggatgcc gcagcggcct gatcaacatt ggtgtttacc agacggaatt ccagcgttgc
    18601 cgtcgcgccc agaatctctt tcgcacgcgc agtgtcctga ataccaggca gttcaacaac
    18661 gatacggtca gcaccctgac gctgaaccac cggctccgcc acgccaagtt ggtttacacg
    18721 gttacgcagg atattaatgt tctgctgcac cgcatattca cgcgcttcac tcagacgagc
    18781 atcgctcatt accgcacgca gctggttgct gccctggctg ctaatcacca ggtccggatg
    18841 gcgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga aagtgatgct
    18901 caggccgtag ttgttttctt tacgaacagt ggtatacggg atgccctttt cgcgcaggtc
    18961 actgcgtagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt ccatatccac
    19021 ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg gctcagcatg
    19081 aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga cgtatttgtc
    19141 acccataacg cccattaatg cttcacgagc gcgcaactgg gtgtcagtgg agtcgaagcg
    19201 cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt cttcttgtaa
    19261 cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg caccagtgat
    19321 ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac cgatgacaat
    19381 caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg caattccctt
    19441 agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg cagctacgaa
    19501 gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt agccgttttc
    19561 cgctactttg gttacgcgac caaccaggcc accgttcgtc agaacttcat cacctttggc
    19621 aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac gcaggatcat
    19681 gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg ggctaccttg
    19741 cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta aatttccctc
    19801 attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc tggtaaaaat
    19861 cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc gccatcaaac
    19921 gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc gcctaatatt tcgttgcaac
    19981 ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa tcacactcag
    20041 gatcgagtgg gccagtatcg ctcttatatt tcgcattgcg gattttcacc acgccatcgg
    20101 tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca aacatatcga
    20161 taccgcgacg tacgccttca accaggtctt ccggtttacc aacgcccatc aggtaacgcg
    20221 gtttgtctgc cggaatttgc ggacacacat gctccagaat acggtgcata tctgctttcg
    20281 gctcacccac agccagaccg ccgacagcgt agccatcaaa gccgatatct accagacctt
    20341 taacagatat atcacgtaaa tcttcgtaaa tactgccctg aattatacca aataacgcat
    20401 ttttgttacc aagactgtca aaacgctcac ggctacgctt cgcccaacgc agagacatct
    20461 ccatagagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca tcaaagatca
    20521 tgacgatatc cgaaccaaga tcgtactgaa tctccatcga cttttccggg tcgaggaaaa
    20581 tcgggtcgcc gttgatcggg ttacggaagt gaacgccctg ttcggtgatt ttacgaatat
    20641 caccaaggct gaagacctgg aagccgccgg agtcggtaag aatcggtcct ttccactgca
    20701 taaaatcgtg catatcgccg tgcagtttca tgatttcctg gcccgggcgc agccacaggt
    20761 ggaaagtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttcc ggcgtcatcc
    20821 cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact acgccacgat
    20881 caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagttca aatttcattt
    20941 tttctccact acgtcagaaa aacagtccaa cgtttaaacc agcgccgcgg aattactccc
    21001 cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca ccgtaactaa
    21061 aaaagcgata tttctcttct accgccgctt tataggcatt catggtgtgt tgataaccgg
    21121 caaaggccga aaccagcata atcagcgtcg actctggcaa gtggaagttc gtcaccagcg
    21181 catcgaccac tttgtactgg aagcccggat agataaagat ttgggtatcg tcaaagaacg
    21241 gttcgataag atcgtttttt gccgcctgtg ccgcgctttc cagcgaacgt actgaagtgg
    21301 tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg tctaccacat
    21361 cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg tcgacgcgca
    21421 ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc acgcctttgg
    21481 cgcgtaattt ttccagcaaa ggctcgtcaa aatgcagacc agcggtcggg gctgcaaccg
    21541 cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct tcgtccggac
    21601 gatcgatata cggcggcagc ggcatatggc cgatgctgtt gaggatatcc agtaccgagc
    21661 gttcatcatt aaattcgact tcaaacagtg cgccgtggcg cgcggtcatt gttgcgttga
    21721 tactttcgtc atcgcccagc aacagttctg cgccaggttt tggcgctttc gaggcgcgaa
    21781 tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac cagcacttca atcttaccgc
    21841 cgctggcttt acgcccaaac agacgcgccg ggatcacgcg ggtattatta aaaaccagaa
    21901 gatcgccggg gttgagctta tcgagtaaat cggtgaaagt accgtgcgtc agcgcacccg
    21961 tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatggga tagtgggcaa
    22021 tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg actcagacta
    22081 aaataagagt cgggtagtct agtgccgggg cgctctccct gcaaccttta cccccctttg
    22141 gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct cgcggaaagc
    22201 tcgctttctg gcaatttact ggctgatttc gtacgcggaa atcctgaaga aagttttccg
    22261 cccgacgtcg tggctggcat tcatatgcat cgacgtattg actgacaatc tgccggaagt
    22321 ccgcgaagca cgggagtggt ttcgtagtga aacgcgccgc gttgcgccta ttacgctgga
    22381 tgtcatgtgg gatcactttc tttcccgcca ctggtcgcag ctgtcgccgg actttccgct
    22441 acaggaattt gtctgttatg cccgcgagca agtgatgacg attttgccgg actcaccgcc
    22501 acgttttatc aatctgaaca attacttgtg gtcagagcag tggctggtgc gctatcgcga
    22561 tatggatttc atccagaacg tgttaaacgg catggcaagc cgccgcccac gtctggatgc
    22621 cctgcgtgac tcctggtacg atttagacgc tcattatgac gccctcgaaa cccgcttctg
    22681 gcagttttat ccgcggatga tggcgcaggc gtcacacaag gcgttataaa acgttagttc
    22741 atccataccg tagccgaaat ggcaggcaaa gccagaatcc cgtcagtcag ttgcccatgc
    22801 ccttctttgc attgccattg cacggcattg agaaacggtg acgcgggtag caccacttca
    22861 ctggcctcgc cacggttgat tgccaccagt acacgttgct gattcagcac gcggacaaat
    22921 accactacgt tatcttccgc atacagcacc tgacagccgc cacgacgcag cgcctgactt
    22981 ttcttacgca gcacaatcat tcgccggtac agcgcgaata acgccgtatc ctgcttttcc
    23041 acctgccagg ggaacggttt acggcaagac ggatcgtttt taccatccag tcccacttca
    23101 tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca gccagaccac cgccagcggc
    23161 aggcgcgcaa tatcccgacc gagcagcgtt ttaaatcgcg cagtatcgtg gctatcgagc
    23221 tgattaaaca tacgtaattg ttgttgatga gaaagccctg cgcggtaatt atccatccag
    23281 gccatacag
//
LOCUS       DAJTYI010000054        23078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYI010000054 DAJTYI010000000
VERSION     DAJTYI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO564_002063"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO564_002063"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6653195.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="OO564_002064"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="OO564_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6653196.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO564_002065"
     CDS             complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO564_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6653197.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO564_002066"
     CDS             complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO564_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6653198.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO564_002067"
     CDS             complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO564_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6653199.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5283..5741
                     /locus_tag="OO564_002068"
     CDS             5283..5741
                     /locus_tag="OO564_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6653200.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5791..6633
                     /gene="purU"
                     /locus_tag="OO564_002069"
     CDS             5791..6633
                     /gene="purU"
                     /locus_tag="OO564_002069"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6653201.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6793..6877
                     /locus_tag="OO564_002070"
     tRNA            6793..6877
                     /locus_tag="OO564_002070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6827..6829,aa:Tyr,seq:gta)
     gene            6924..7055
                     /locus_tag="OO564_002071"
     ncRNA           6924..7055
                     /ncRNA_class="other"
                     /locus_tag="OO564_002071"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO564_002072"
     CDS             complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO564_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6653202.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO564_002073"
     CDS             complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO564_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6653203.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO564_002074"
     CDS             complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO564_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6653204.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10158..13901)
                     /locus_tag="OO564_002075"
     CDS             complement(10158..13901)
                     /locus_tag="OO564_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6653205.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO564_002076"
     CDS             complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO564_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6653206.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16024..17820
                     /gene="narX"
                     /locus_tag="OO564_002077"
     CDS             16024..17820
                     /gene="narX"
                     /locus_tag="OO564_002077"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6653207.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17813..18463
                     /gene="narL"
                     /locus_tag="OO564_002078"
     CDS             17813..18463
                     /gene="narL"
                     /locus_tag="OO564_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6653208.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO564_002079"
     CDS             complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO564_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6653209.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20043..20396
                     /gene="ychN"
                     /locus_tag="OO564_002080"
     CDS             20043..20396
                     /gene="ychN"
                     /locus_tag="OO564_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6653210.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO564_002081"
     CDS             complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO564_002081"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6653211.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO564_002082"
     CDS             complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO564_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6653212.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21793..22893
                     /gene="chaA"
                     /locus_tag="OO564_002083"
     CDS             21793..22893
                     /gene="chaA"
                     /locus_tag="OO564_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6653213.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5777 a         6032 c         5546 g         5723 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg tttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaatgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa gacaatttaa
     1981 taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg
     2041 atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa attccgtgcc
     2101 aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta atttattacc
     2161 gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt cgatcagcat
     2221 atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt ttgccagcaa
     2281 cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag acggcgcaac
     2341 atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg gcgctaattc
     2401 aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt caaccatgat
     2461 ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat cctgtgacaa
     2521 atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg gttcatcacc
     2581 cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac cctgacgaac
     2641 ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca gttgacgttt
     2701 tacacgtttt tccagcattg cctccagttc aaaactggta tcaaagtggt tttcaataga
     2761 gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa tacattcatt
     2821 cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg ggatggcttt
     2881 cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt ttttgacttt
     2941 cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac gtgttcatat
     3001 ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga aaaggaggcg
     3061 agatagcata attaagacaa ttacttatac gaatggcgac gatagtacca ccagccgaca
     3121 ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag acaacatcaa
     3181 gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc gctggctcaa
     3241 ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga tttgcacctg
     3301 gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga gatttttcag
     3361 ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg cctgacgaag
     3421 taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat tttggtgtgc
     3481 aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc
     3541 attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc
     3601 ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacggt aattaacccg
     3661 gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc
     3721 ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt cctcaacgcg
     3781 cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc gattcagatc
     3841 tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg caatatctgc
     3901 catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt tacgtatatg
     3961 ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca tcaggtctgg
     4021 agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta ccgttgtcgc
     4081 tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat cttcaacaat
     4141 gagaatctgt tttccgacca atggctgcgt catgttctct cccctgaccg gctttactca
     4201 atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta gataaaaatt
     4261 ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt tccactgcca
     4321 gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc
     4381 aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt ttaaggcggt
     4441 tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct gtcacagccc
     4501 gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt agagaatcac
     4561 cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc atcaaatgag
     4621 catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc gtaagagaaa
     4681 taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt gcaacgggtg
     4741 ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg ccttcagtaa
     4801 accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga cgggaacaat
     4861 tttcgatctc tgtttccggc attatttcgc gatattgatt gaagacacgc tcgccgcgca
     4921 gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa taactgaaag
     4981 aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg cccaccagcg
     5041 aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt agcgcattaa
     5101 taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc cctattttta
     5161 tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca tcgctactgc
     5221 tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca gggagattat
     5281 tggtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt
     5341 atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg cgttactgcg
     5401 cttttgtgat gcaagacgct gattatttaa taaagacctg gcatccctct tgtggagcag
     5461 cggcgttacg tgccgaattg atgaccggat ttgcgcatac tgaatggttg ggattgaccg
     5521 tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt gttgctcgat
     5581 ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg
     5641 gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat ccctgccctt
     5701 gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg gtacggttta
     5761 gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa agttctgcgt
     5821 actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg ctacaagcac
     5881 gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg cttttttatg
     5941 cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
     6001 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg
     6061 gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggtctg
     6121 gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct ggttgagcgt
     6181 tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga gcacgatcaa
     6241 aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc gaagtatatg
     6301 cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa tattcaccat
     6361 tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga acgtggtgtg
     6421 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc
     6481 atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt
     6541 cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc gcaacgcgtc
     6601 tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac tggttacctt
     6661 taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta acactttaca
     6721 gcggcgcgtc atttgatatg atgcgccccg ctccccgata agggagcagg ctagtaaaaa
     6781 gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc
     6841 atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag ccccggaatt
     6901 ctcaaacgaa tctgcaatca aatattctgc ccaagcgggg aaggatgaga agcttcgacc
     6961 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
     7021 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttgaata atcaaaacct
     7081 gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca atatcctccc
     7141 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
     7201 accccgccga agcagggtct atgtgaacta aaattcgttt agtgacgagc gcgcaccagc
     7261 tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag acgcgagaac
     7321 gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat aaacgccaca
     7381 ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc ccagccaacc
     7441 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt cagcaggccc
     7501 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
     7561 cgcacgcgtg ggctgaacat acgacgtttc agcaaaagca cgccgccaat cagacacagc
     7621 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
     7681 gcttcataca tccagtgcgg cgtcaacata ccgaagaagt gaccgacaaa aatccccaga
     7741 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
     7801 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
     7861 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
     7921 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     7981 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     8041 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     8101 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     8161 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     8221 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     8281 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc tcgtactgcg
     8341 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     8401 gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactggacc tgggcatcga
     8461 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     8521 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     8581 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     8641 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     8701 tgagccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     8761 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     8821 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     8881 ggtatcaact ttaccgtcaa cagtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     8941 tttcagcgca cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     9001 taccggaata cgcaagcttt cgacgtctgg cagaattccg ttgctgccca gctcgcccgc
     9061 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     9121 atattctgga tgcagcggca gcgccagttt ccattccatc gccattttat aaaccggcga
     9181 ctgctgtgca gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     9241 tttcggatcg ttcggatcga ggaacacgtc cagctgacgc tggtagagat ctttctcgtt
     9301 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     9361 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     9421 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     9481 acatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     9541 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     9601 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     9661 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttatcaaact caccgcccag
     9721 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     9781 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     9841 atcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcaaagat
     9901 tttacccagc agcatggctc ggttacccat gcgcggttgc agtttgccat tgattttacg
     9961 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctggcccgg
    10021 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
    10081 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
    10141 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
    10201 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
    10261 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
    10321 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
    10381 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
    10441 cgctggctga caaccgcacg ggcagtcagt gcaccgttgc tgttgaagac ttcaatccag
    10501 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
    10561 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
    10621 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
    10681 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
    10741 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
    10801 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
    10861 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc
    10921 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
    10981 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagcgt caggatcatc
    11041 tctgccgcat caattgcggt gttcagcatc ggctggcctt ttgccggacc ttccgctttg
    11101 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
    11161 ccgccgttac cgattttctc catcagcggg ccgatagagg taaagcgttc gtaagttgcc
    11221 ggataatcgc gctctacgac cataatgtgc ggtgcagttt tgcctgggat caggtcgcat
    11281 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcgtgc
    11341 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
    11401 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tagcttccca ggccggatcg
    11461 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
    11521 tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
    11581 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
    11641 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
    11701 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
    11761 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
    11821 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
    11881 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
    11941 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
    12001 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
    12061 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
    12121 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
    12181 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
    12241 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
    12301 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
    12361 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
    12421 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
    12481 acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
    12541 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
    12601 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
    12661 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
    12721 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
    12781 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcagcata
    12841 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
    12901 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
    12961 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
    13021 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
    13081 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
    13141 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
    13201 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
    13261 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
    13321 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
    13381 ttagatgcgg cgatcagttc gttcacctcc tgccaagaag aacgaacaaa tccaccgcgt
    13441 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
    13501 tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc
    13561 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
    13621 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
    13681 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
    13741 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
    13801 tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa ggtttcaccc
    13861 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
    13921 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta acgctctgaa
    13981 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
    14041 agatacccct ttaggataat taacagttac agcataacat gctgtaaatt aagacgtttt
    14101 atacctgtag ttatttaaac tattaagatt tttatgcaaa tgggtattaa ggagtattcc
    14161 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataagcca tataacacta
    14221 atatgttgta actaaatgac gcataaaaaa agcgcggcat catgccgcgc aaaggataat
    14281 caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaagta atcactacgc
    14341 aggcgatata gaaaatcaaa aatactttca ttgcacctac tggcgaaccc gttaatgcca
    14401 gcgagctacc aaacgctttc gggataaaga agccaccaat cgcgccaatc gcagagatga
    14461 aacccagcgc cgctgccgtg ccggttgccg cttcacgcat cgcacgttcg tcagaacccc
    14521 cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag
    14581 tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca
    14641 tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg
    14701 ccatcagaat aaagttcacc agcgtgacac gagttccgcc cagacggtca gataatgcac
    14761 cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct
    14821 gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc cgcggagaag ccgatgaagg
    14881 agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg
    14941 gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg
    15001 caatggtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct
    15061 cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata
    15121 ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gtttcccaga ccaccattca
    15181 gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg
    15241 atgcgaagtt cgcgccagca aaaccgcata gcagagagat gatgatgaag acgctataag
    15301 gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg
    15361 tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag tatggaacac
    15421 gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa
    15481 aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc catacgcaaa
    15541 acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata cgttgaccgc
    15601 gttgttgcca gaacgcagga tcttccggtc gccaatctgt tatgacagct ccagtagccc
    15661 tttcggaggc agatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc
    15721 ttagtgtctg gagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat
    15781 cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt
    15841 tcacggtgct gtagaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata
    15901 aggagtaact ctttccggct atgggtatac ttcagccaat agccgagaat actgccattc
    15961 cagaatatat cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaaggggtt
    16021 tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg
    16081 ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc
    16141 gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt
    16201 ctgttggcgg cagtgccatt aagcgagaaa gacaagccct taattaaaga gatggaacaa
    16261 acggcattta gcgccgagtt gactcgagca gcagaacgag acggacaact ggcgcaatta
    16321 cagggtttac aagattactg gcgtaatgaa ctgatccctg cgctgatgcg tgcacaaaac
    16381 cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca gctggtatct
    16441 ggttttgacc gcaccacgga aatgcgtatt gagacagtgg tactggtcca tcgggtaatg
    16501 gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta
    16561 caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gccatcgcga ttttacccaa
    16621 cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt gaacaatatg
    16681 tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc
    16741 gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat
    16801 tcccgcgccc cgctgtgtga acgcctgtca ccggtactca acggcttaca gaatttaacc
    16861 ctgctacgtg atatcgaact gcgggtgtat gacactgatg atgaagagaa tcatcaggag
    16921 tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc
    16981 gtattacccg ttggtgatcg cggcacgacc ctgaagtggc ggctggctga ctctcatacg
    17041 cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa
    17101 caactggtgg ataccctggt tgaacaactt accgccacgc tggcgctgga tcgccatcag
    17161 gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat
    17221 gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag
    17281 ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat
    17341 gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct
    17401 ggattacgtc cggcgctgga agcgagttgc gaagagtaca gcgccaaatt tggcttcccg
    17461 gtgaagctgg attatcaatt gccgcctcgt ctggtgcctt cacatcaggc aatccacttg
    17521 ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc gagtgaggtc
    17581 gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc
    17641 ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt
    17701 gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg
    17761 gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac ccatgagtaa
    17821 tcaggaaccg gctactatcc tgctgattga cgatcacccg atgctgcgaa ctggcgtaaa
    17881 acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta atggcgaaca
    17941 gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc tcaatatgcc
    18001 cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct cagggcgcat
    18061 tgtgatattc agcgtctcta accatgaaga agatgtggtc accgcactga aacgcggcgc
    18121 ggatggctat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat tgcatcaggc
    18181 agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg ccagcttgcg
    18241 cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg agcgcgatat
    18301 tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc tggatatcac
    18361 cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc tcaagtctcg
    18421 cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca
    18481 gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg
    18541 agacacgctg cccctggtta tcttccacca ccacagacaa tcgccagtga ttgcttgccc
    18601 cttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg ttggcttgtg
    18661 cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt
    18721 aacgactgcg catttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggcg
    18781 gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggtagattat
    18841 ttcgctgcgg attgtcatag cgactaccac gtaacgactg actttcggca acctcgcccg
    18901 cagaaagttg ttttttgagc ggtacaccaa agcggtaatt aagattcagc ccgaggttat
    18961 tttgattttc gccactttcc ccctgtttat gctgggccgt cacagtgact aatggcacag
    19021 gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta ccaaagttaa
    19081 acaaatcaac acgatcacca aaatactgtt ctaggctgac actggtattg aggtgttgat
    19141 agaacggcat gcgcatccga gctgtcaggt cgtacccgcg cgccatccgt tgttcctgcg
    19201 tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat aatcgcaaat
    19261 attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc tgcaagttgt
    19321 cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc ccaacgccca
    19381 cattgctcac caacccatta tcctgctgag taagaccaag ctggctccag gtgagataac
    19441 gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt
    19501 tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct
    19561 gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct tttgcctgct
    19621 cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc acaatttcag
    19681 caaagtgttt ttccccatca tgattttcgg gagccatgcc taaatccggc agaccatcat
    19741 tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac
    19801 cgcctgctac aagcaagagt aaataaaacg ggataatgcg aggaacgaaa cggctcaaaa
    19861 cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg attactggca
    19921 acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaatgtgac cgattggtca
    19981 gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata caggagtaaa
    20041 taatgcaaaa aatcgtgatc gttgccaatg gcgcacctta cgggagcgaa tccttgttta
    20101 acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat ctgcgtctgt
    20161 tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg gaaggctaca
    20221 acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa ttgtgcaaaa
    20281 cctgtaccga cgggcgcggg attagcacac ttcctctgat tgatggggtg gaaatcggta
    20341 ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca ttttaatgtt
    20401 taagggggcg tctgacgccc cactatgagg gaagtttact caggcgaatc ccggccttaa
    20461 cacaccatcc ggataattct ccgccagtag tttttttacc gataccagca aatcattcag
    20521 cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca ggtattgtgc
    20581 attggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct gagcgcgagt
    20641 gtcagattca tattctggat gtcgcgggtc cataataaac acaatggcgt ttactgtgcg
    20701 tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa tcatctcgcg
    20761 cttccacaac agggttagtt cctgctccag cgtctcttct ggtagtcgat aggcgacgcc
    20821 tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg cagtcccgcg
    20881 cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca accagtgtac cggtgcatga
    20941 ttcggtaaac tccagtgccg gattccacat caacgatcca taaccgaaga tccacaccgg
    21001 tccctcatca ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt gttctgctga
    21061 ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat tcatcaagaa
    21121 atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca actttttggt
    21181 gtcttccaga catttttacc gctcattttg aaaccactaa cgggacaata tgcaatccac
    21241 ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg ttttacgatt
    21301 ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca gcccaggcca
    21361 ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcttcttta tctttatatt
    21421 gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac ggtagaacgt
    21481 gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac tccttttaca
    21541 aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc caaaacataa gaattttcat
    21601 gtcagactgt tcttaagaaa cgctaaaaca aacattgtct aaacgtaata cttattttta
    21661 tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt accgatatcc
    21721 agacactttc tgttattttt cctacctatt ttttacattt tcgataaatt catctgcaaa
    21781 gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa ggagacttcg
    21841 cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagtag ccagacacta
    21901 ccagtggtca ttgccatcaa tcttcttgcg cttattggtt ttttaagtag cgcctttagt
    21961 gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg ttcgcttatt
    22021 cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat ggcaaccggc
    22081 gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat tgttaccggt
    22141 gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca atatatgaat
    22201 ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat cgtactggta
    22261 tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact ggtagcatta
    22321 atttctgcgg caatgtatgg cgtatttttg ctgatccagg ccaaaacgca tcaaagtttg
    22381 tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcaccacgg taaaccgtct
    22441 gcccatagca gcctgtggca tgctatctgg ttgattatcc atctgattgc cgttattgca
    22501 gtcaccaaaa tgaacgccag ctcgctggag acattgctcg acagcatgaa tgcccctgtt
    22561 gcctttactg gcttcctggt ggcactgttg attctgtcgc cggaaggttt aggtgcgtta
    22621 aaagcagtgt tgaacaacca ggttcagcgc gcgatgaatc tgttctttgg ttcagtgtta
    22681 gcaacaattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac gggtaacgaa
    22741 ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt agtgctgtgc
    22801 catatctcct tctccaccgg acgtactaac gtgctcaatg gcgcagcgca tctggcactg
    22861 tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg atgacggaaa
    22921 tcgggagaga taagataaat tcctgcttat tgtatttctt catactacct ggacagctcg
    22981 ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac agctgttgaa
    23041 aagccggaat gtactcttta ttgcaaacat ttcaggga
//
LOCUS       DAJTYI010000055        22821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYI010000055 DAJTYI010000000
VERSION     DAJTYI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..487)
                     /locus_tag="OO564_002084"
     CDS             complement(<1..487)
                     /locus_tag="OO564_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6653214.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QA"
     gene            705..1565
                     /gene="tesB"
                     /locus_tag="OO564_002085"
     CDS             705..1565
                     /gene="tesB"
                     /locus_tag="OO564_002085"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6653215.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1614..2900)
                     /gene="amtB"
                     /locus_tag="OO564_002086"
     CDS             complement(1614..2900)
                     /gene="amtB"
                     /locus_tag="OO564_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6653216.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2930..3268)
                     /gene="glnK"
                     /locus_tag="OO564_002087"
     CDS             complement(2930..3268)
                     /gene="glnK"
                     /locus_tag="OO564_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6653217.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3449..5230)
                     /locus_tag="OO564_002088"
     CDS             complement(3449..5230)
                     /locus_tag="OO564_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6653218.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5223..6995)
                     /locus_tag="OO564_002089"
     CDS             complement(5223..6995)
                     /locus_tag="OO564_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6653219.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7025..7483)
                     /gene="decR"
                     /locus_tag="OO564_002090"
                     /pseudo
     CDS             complement(7025..7483)
                     /gene="decR"
                     /locus_tag="OO564_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7637..8455)
                     /gene="cof"
                     /locus_tag="OO564_002091"
     CDS             complement(7637..8455)
                     /gene="cof"
                     /locus_tag="OO564_002091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6653220.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8555..10254
                     /locus_tag="OO564_002092"
                     /pseudo
     CDS             8555..10254
                     /locus_tag="OO564_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10319..11014
                     /gene="queC"
                     /locus_tag="OO564_002093"
     CDS             10319..11014
                     /gene="queC"
                     /locus_tag="OO564_002093"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6653221.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11066..11464)
                     /gene="fadM"
                     /locus_tag="OO564_002094"
     CDS             complement(11066..11464)
                     /gene="fadM"
                     /locus_tag="OO564_002094"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6653222.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11558..11929)
                     /locus_tag="OO564_002095"
     CDS             complement(11558..11929)
                     /locus_tag="OO564_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6653223.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12080..13951)
                     /gene="ppiD"
                     /locus_tag="OO564_002096"
     CDS             complement(12080..13951)
                     /gene="ppiD"
                     /locus_tag="OO564_002096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6653224.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14143..14415)
                     /gene="hupB"
                     /locus_tag="OO564_002097"
     CDS             complement(14143..14415)
                     /gene="hupB"
                     /locus_tag="OO564_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6653225.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14624..16978)
                     /gene="lon"
                     /locus_tag="OO564_002098"
     CDS             complement(14624..16978)
                     /gene="lon"
                     /locus_tag="OO564_002098"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6653226.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17166..18440)
                     /gene="clpX"
                     /locus_tag="OO564_002099"
     CDS             complement(17166..18440)
                     /gene="clpX"
                     /locus_tag="OO564_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6653227.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18566..19189)
                     /gene="clpP"
                     /locus_tag="OO564_002100"
     CDS             complement(18566..19189)
                     /gene="clpP"
                     /locus_tag="OO564_002100"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6653228.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19436..20734)
                     /gene="tig"
                     /locus_tag="OO564_002101"
     CDS             complement(19436..20734)
                     /gene="tig"
                     /locus_tag="OO564_002101"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6653229.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21078..21395)
                     /gene="bolA"
                     /locus_tag="OO564_002102"
     CDS             complement(21078..21395)
                     /gene="bolA"
                     /locus_tag="OO564_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6653230.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21700..22278
                     /locus_tag="OO564_002103"
     CDS             21700..22278
                     /locus_tag="OO564_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6653231.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22322..>22821
                     /locus_tag="OO564_002104"
     CDS             22322..>22821
                     /locus_tag="OO564_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653232.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLA"
BASE COUNT         5403 a         6340 c         5482 g         5596 t
ORIGIN      
        1 tggcctgaac cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc
       61 cgccctggtt gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg
      121 taatcgccgc actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca
      181 gaacaaagct gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt
      241 ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg
      301 gcagtgcgac tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag
      361 gttgttgtgt tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc
      421 ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga
      481 gtttcattca tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga
      541 gtggttaaca aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa
      601 ttagcccgta attcagacta ttgcacccat cggaccagtg gcaaatttgc gttatactca
      661 actcactttg gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa
      721 aaaatttact gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga
      781 gtgaagattt aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg
      841 ctgcaaaaga gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc
      901 gcccaggcga tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca
      961 gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg
     1021 cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag
     1081 cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc
     1141 cagtgcttaa agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc
     1201 ataacccact gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg
     1261 gtagcgtgcc agatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta
     1321 acttcctgcc ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga
     1381 ttgccaccat tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc
     1441 tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt
     1501 atacccaaga cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca
     1561 attaaaaaaa tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg
     1621 ttataggcat tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc
     1681 acacgtaggc caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac
     1741 cagacgatcg taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca
     1801 ccttcagcga agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag
     1861 ccgacaatgc cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc
     1921 aagcgtttga gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc
     1981 aacgcgccgc caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc
     2041 gcgccagaac acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc
     2101 cagccaagaa ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt
     2161 tcattcgccg tgcccgctga cccggcgtta aagccaaacc aaccgatata gagaatggca
     2221 gtcccggtga agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcccacg
     2281 cgtttgccta tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc
     2341 gtgccacccg cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata
     2401 tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag
     2461 aagcggattc gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat
     2521 ccctgaaacg ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg
     2581 ttttttagca tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc
     2641 gaataaccgt aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc
     2701 agcatcgaca gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga
     2761 atagtcataa acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg
     2821 gccaccgcag gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt
     2881 tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg
     2941 cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt
     3001 tgccgtcgcc aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga
     3061 gttggtcatc agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt
     3121 ccgccccccg gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga
     3181 cggtcaggcc ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg
     3241 gtttgattat cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt
     3301 ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc
     3361 aggaagttaa gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt
     3421 ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc
     3481 tggctgccag ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg
     3541 ccgccagcag ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa
     3601 tggtgtcggc atcaataatt gttgataagc ggtgagcaat caccaccagc gtggtatgtt
     3661 cacgcaccgc cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg
     3721 ttgcctcatc aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca
     3781 gttgcttttg cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat
     3841 cactcatgct gcgcgccagc tcagccagtt gcacggtttc cagcgcctgc cagacgcgtt
     3901 cttcggagat atcccgcccc agcgtcacgt tggcgaggaa ggtatccgcc agcaccaccg
     3961 gatcttgctg caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg
     4021 gacgaccatc aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg
     4081 caagggtact tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca
     4141 cagagagatt aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat
     4201 cgacttcgat ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc
     4261 cgtccatcag ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt
     4321 gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca
     4381 acacgcccac ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa
     4441 gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg
     4501 tttgcatcct agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg
     4561 cctgctgacg aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt
     4621 tgatatccgc cagataggcg cgcacacggc ggacaatcgg cgtgttgtaa cgctggtata
     4681 tcaccattac caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt
     4741 cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg
     4801 ccactacggt aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggagatca
     4861 cctgcccgac gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca
     4921 cgtcggtacg caactgttgc actacgccta ctgccgcccg attaaacagc agcgactgag
     4981 cgtaatgtag cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa
     5041 ccactttcaa cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca
     5101 gcggcccact gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg
     5161 gtttacgcca cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa
     5221 aactacgcat cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt
     5281 tgttgatagc gatacatatc gcgataccag ccgctttgtt gcgccagctc atcatgattg
     5341 ccacgctggg cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc
     5401 gaaaggcgat gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc
     5461 aggatctggt gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt
     5521 tccgcgttga ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc
     5581 atcacgccgc gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca
     5641 tgtacgctgg ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc
     5701 agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc
     5761 aggcggctac gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata
     5821 tccccctcgc tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa
     5881 ccagtcggcc cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc
     5941 agcgcaggat ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca
     6001 cgcccttccg gcacgagttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg
     6061 cgaatacggc tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc
     6121 agcattggcc aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt
     6181 aaactgccct gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca
     6241 atcgcgatat agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt
     6301 ttgccggtat cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag
     6361 gctttgatca tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac
     6421 gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc
     6481 gccatcactg gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac
     6541 atcataatca acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct
     6601 ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg
     6661 cgatgacgca ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc
     6721 agttcaacag ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg
     6781 agataaacca caacggcaat cagcaccatg gtggcgatcc acatcaggat cagcccggta
     6841 gtaaagtgtt gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt
     6901 tgcagcatcg cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga
     6961 cggaaatacc agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg
     7021 atatttattc gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag
     7081 tgacgtccga cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt
     7141 tcatgtcggc aacctagacg cgcatcagat aatcgtattc accagccatg cgccagaacc
     7201 ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt
     7261 gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca
     7321 gcagggcgac tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc
     7381 agggggtggt tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct
     7441 gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa
     7501 tttttttctc ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt
     7561 taaacaccac ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt
     7621 gctggaaggg atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg
     7681 tcaaatagtg agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct
     7741 ccgcgcgcag ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca
     7801 tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat
     7861 gttgggtcag caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc
     7921 aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct
     7981 gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg
     8041 gcattttttt gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca
     8101 acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc
     8161 gggtatccca ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat
     8221 gtaaaagttc accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat
     8281 ccagcgatag cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag
     8341 tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat
     8401 gatggtcagg catcaataaa gtgccatcca tatcaaatgc tgccagacga gccatttctt
     8461 tctccactct gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat
     8521 agtgaatagt taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca
     8581 gtatcaacgt ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact
     8641 ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga
     8701 ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt
     8761 tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa
     8821 gcagcaagat gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca
     8881 gccgtttatg ggcggacaat ggcaaacgat acacccacgt tgcgtattcc ctactatcgc
     8941 ccgctcgaac cgctacaacc aggctttttg cccggccgtg ccgagcagca tctcgccggg
     9001 cagatatttt ccggcctgac ccgcttcgat aataatactc agcgcccgat tggcgattta
     9061 gcgcatcact gggaaacctc tactgacggg ttacgctggg acttttatct tcgttcaacc
     9121 ctacactggc ataacggcga tgcagtaaaa gcctcacact tacaccagcg attattgatg
     9181 ctgttacaac tgccagcact ggatcaatta tttattagcg tgaagcgtat tgaagtcacc
     9241 catccgcagt gtctgacctt ctttttacat cgccccgatt actggcttgc gcaccggctg
     9301 gcgagctatt gcagccatct ggcgcatccg caattcccac tgatcggcac tggtcctttt
     9361 cgcttaacac aattcacagc ggagctggtg cgcctggaga gccatgatta ttaccatttg
     9421 cgtcatccgc tgcttaaagc ggttgagtac tggataactc cgccgctttt cgaaaaagat
     9481 ttgggaacca gttgtcggca tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa
     9541 cgggtcagcc aggtcagtag tggcatcagt ttaggttttt gctatttaac gttgcgcaaa
     9601 agtccccgac tctccctctg gcaggcgcga aaagtgatct ccattattca tcaatccggt
     9661 ttattacaaa cgttagaagt cggagaaaac ctgatcaccg ccagtcatgc attactgcca
     9721 ggctggacta ttccgcactg gcaagtaccg gatgaagtca aactaccgaa aaccttgacg
     9781 ctggtttatc acctaccgat agaacttcat accatggcag aacgcctaca ggcgacactg
     9841 gcagcggaag gctgtgaact cacaattatt tttcataacg caaaaaactg ggacgacacg
     9901 accctactgg cacacgcaga cctcatgatg ggcgacagat taattggcga agcaccggaa
     9961 tatactctgg agcaatggct acgttgcgat ccactatggc cacatgtttt cgacgctcca
    10021 gcatatgcac atctgcaatc gacgctggac gcggtgcaag taatgcctga tgaggaaaac
    10081 cgatttaatg ccctgaaagc ggtttttagc cagttaatgg cagacgcgac gctgacgccg
    10141 ctgttcaact atcactatcg cattagtgcc cctcccggcg tgaacggtgt gcgactgaca
    10201 ccgcgcggct ggtttgaatt taccgaagcc tggcttcccg cgccgtcgca atgaatagct
    10261 ggtccgggtt aatcgcaggc gttaccataa cgcttttatt gttcaagcag gattatctat
    10321 gaaacgtgct gtcgttgtgt tcagtggagg ccaggattcc accacctgtc tggtgcaggc
    10381 attacaacaa tatgatgaag tccattgcgt gacgttcgat tacggtcagc gacatcgcgc
    10441 agaaatcgac gtggcacgcg aactggcgct gaaactggga gcacgcgcgc ataaggttct
    10501 tgatgtaacg ctgctcaacg agctggcggt cagcagcctg acacgtgaca gcattccggt
    10561 gcctgattat gaacctgaag ccgatggcat cccgaatacg tttgtcccag ggcgtaatat
    10621 tttgttcctg acgctggcgg caatatatgc gtatcaggta aaagcagaag ccgtaattac
    10681 cggcgtctgt gaaacggatt tctccggcta cccggattgc cgcgatgagt ttgtgaaagc
    10741 actaaaccat gccgtcagtt tgggcatggc gaaagatatt cgttttgaaa cgccgctgat
    10801 gtggattgat aaagcggaaa cctgggcgct ggcagattat tacggcaaac tggatttagt
    10861 ccgtaacgaa acgttgacct gctataacgg cattaaaggc gacggttgcg gtcattgtgc
    10921 ggcatgtaat ttacgcgcca acggtttgaa tcattatctg gccgataaac cgacggtgat
    10981 ggcagcgatg aagcagaaaa ccgggttgag gtaattattc cgggtgtcgc cggatgcggc
    11041 ttgagcatcc ggcaccacaa aacgtttact taaccatctg ctccagcttt tcgcgcaatt
    11101 ccccttccag agctaatgct ttctgcgttt taagatcaat acaaacaaac gtaataagcg
    11161 catccgctac cacctgccct tccggctcca gtgtaatgac ctggcttaag atgccgcttt
    11221 taccgtttaa ttgctgcaac tgactggtaa tagttaacag gtcgcttaat accgctgggc
    11281 gacgatagtt aatattgata ttgaccacga cgaaggcgat gttatgggcc gtcatccact
    11341 gaaaactgtc gctattttcc aacccatccc agcgggcttc ttcgagaaat tcaaggtagc
    11401 gggcgttgtt gacgtgctgg taaacgtcga gatgatatcc acgaactttg atttgtgttt
    11461 gcatagcgca ataaccttac gttgttgtta taagtacaga ggtcataact ggtatgacct
    11521 cttcagtctg gcaaaaaatt gccactatgc aaattaatta cagggttaat accgccagat
    11581 tacgttccac cagcaaattg cccatccccg gcacctgctt tagatcctcc accgttttaa
    11641 acggaccgta ctcttcgcga taactgacaa tcgcctgcgc tttcttcagg ccaacgccat
    11701 tcatcgcgcg ggctagctct tccgcgctgg cattattaat gctgacccgg gtgccttctt
    11761 cgtcactggc tttcgccggt actgctgctt tactttgtgc tgcaggagct tccgctttgg
    11821 tttctaccgc cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg
    11881 cacaggccag ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc
    11941 cttgtttgtt aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg
    12001 ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt
    12061 ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct
    12121 ctttacgcag gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac
    12181 cctgcaccat cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg
    12241 ccagcagaac cacattacct tgcatatcag tcgccatacc gtagctcggt ttgtctttcg
    12301 ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg
    12361 ttttcggctc gccaaatttc agaccggcag cctgcatagc ttccgcacct ttgccggctt
    12421 tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct
    12481 gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt
    12541 gctcgctgac gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga
    12601 tgcccgacgc gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct
    12661 tgaagttcaa ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa
    12721 cgccggcagc ttgctctgca ccggccagag actcggtttc gttgcttgcc gcatcgctca
    12781 ctttctgctg cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa
    12841 tgtcgtcacg tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta
    12901 caatcaggaa accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac
    12961 cagcattttt cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt
    13021 tacgggcaga gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt
    13081 tattcagctc atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt
    13141 agcgggtacg ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat
    13201 ccgcatcgct aaccggttgc tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga
    13261 attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt
    13321 cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc
    13381 gttgttgcgc gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac
    13441 cggcaacgcc gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact
    13501 gatcggcggt catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc
    13561 cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac
    13621 ccagtttcag ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca
    13681 cctgttgacg cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc
    13741 ccagctgttg ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc
    13801 tgatttcctg gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac
    13861 tcacgccggt caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac
    13921 tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact
    13981 gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca
    14041 attgtcagcc cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag
    14101 atgcgccctt gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt
    14161 cagtgcttta cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc
    14221 ggtctgcggg ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc
    14281 taccagtgct acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc
    14341 taacgcacgg ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa
    14401 ttgagattta ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa
    14461 ggtacgaacg cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag
    14521 ggcaagcccg atccgctatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta
    14581 gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa
    14641 cctgcatgcc agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca
    14701 caggatgaat gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat
    14761 tttcgaacgg aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt
    14821 tcaaaccacc gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat
    14881 cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg
    14941 cactcggacc atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt
    15001 cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg
    15061 cctgaataga ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac
    15121 ccggaacaca tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac
    15181 cggttacctg accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac
    15241 cgaggtagtc atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca
    15301 gtaactgctt aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc
    15361 ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct
    15421 cacctttttt cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt
    15481 tcagtttttc atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg
    15541 gtgccggaat gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat
    15601 ccacttccag gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca
    15661 gtgcagaggc cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga
    15721 acagcgggtt tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac
    15781 cgatgtaagt acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca
    15841 tacggacata tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta
    15901 cccccggcgg ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa
    15961 ccgcaaggta ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa
    16021 ggatttcctg cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct
    16081 gtaccatcca gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca
    16141 ttttcagctt ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg
    16201 cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt
    16261 ctttctgaat agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct
    16321 gctttttaac gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca
    16381 tcattgccat cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt
    16441 tgtcagccag tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt
    16501 cgatgctatt cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc
    16561 cttcgaactg gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg
    16621 gcgactccag atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa
    16681 tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca
    16741 tctgcaatat agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg
    16801 ttgaagcttc tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca
    16861 gacaacggat agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca
    16921 ccacatcgcg cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag
    16981 agctctctct tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca
    17041 tctattaaca tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag
    17101 aggaaaaatt aaaggggaga taaaatcccc cctttttgtt taactaattg tatgggaatg
    17161 gttaattatt caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg
    17221 ctttgaccat caattaccga ctcgtcaata accacttttt cgacgtcttc catggacggc
    17281 agatcgtaca tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg
    17341 gttttacgcg ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga
    17401 tccacgcctt ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct
    17461 ttgaggatct gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga
    17521 cgaccaatga actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc
    17581 agcagctcgc cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg
    17641 ccggagccgg tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca
    17701 caaataaaca ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc
    17761 ccaccttgcg gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt
    17821 actccttcac cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg
    17881 tcgatttcat cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt
    17941 tgcaacagct tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc
    18001 gtggttgcgt cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc
    18061 agcgttttac cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca
    18121 ccattgctgg tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc
    18181 acttttttcg cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc
    18241 ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg
    18301 atgtcgttac ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc
    18361 ttgcgcactt catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca
    18421 tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac
    18481 gacgcaaatg ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tagataagta
    18541 tagcggcaca gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac
    18601 cgtattccac cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct
    18661 gttctaatga ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca
    18721 gaatttcacg agcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt
    18781 ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca
    18841 acaagaaagc gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga
    18901 taaactgcat ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa
    18961 tgtacagata gatatctttt tctgggtttt ccgcttccag gaacagcatc tgcgccacaa
    19021 tcaggttagc catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta
    19081 gacgagaata gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca
    19141 ccagcgccat atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct
    19201 ggataaaatt gaaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta
    19261 gtccctgtgt tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac
    19321 gctaacaccg aaagaggctt ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc
    19381 caccacctgg cggtgacggg cctttgtgcg aatttagcgc gttatgctgc gtaaattacg
    19441 cctgctggtt catcagctcg ttgaaagtgg tttctttttc agtcactttc gctttcgcca
    19501 gtacagcttc aacagcctgt tcttccagag caacattgcg catgttgtcc atcagttctt
    19561 tgtttttgct gtagaactcg ataacttctt tcggatcttc gtacgcagaa gccatctctt
    19621 cgatcaggcc tttcacgcgc tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc
    19681 ccagcagcag gccaacaact acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca
    19741 gttccagagc ttgtttttcg ttgccaccga aacgctgtgc agcctggcga cgcagaacgt
    19801 cgatttcgct gtcgatcagc gcagccggta cgtcgatgtc gttagctttt accagacctt
    19861 cgatcgcctg agacttaacg cggttacgga tggcgctctt cagctcgcgc tccatgtttt
    19921 tacgcacttc agcgcgcaga ccttctacgg aaccatcttc aacgccgaaa cgtttgatga
    19981 attctgcagt cagttccggc agttcacgct cttcaacttt cttcaggttg atagcgaatt
    20041 tcgctgcttt acctttcagg ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg
    20101 tgaactcttc gccagctttg tggcctttga taccgtcttc aaagcccggg atcatacgac
    20161 cctggcccat cgccagtacg aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta
    20221 cagaaccggt gaagtcgatg gttacgcggt cttctgcttc aacagcgccg tctttttctt
    20281 tccaggtcgc ctgctgttta cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca
    20341 cttcaacgat cggtttttca acttcgatcg cttccagacc ctgcagttca acttccggat
    20401 aaacttcaaa ctctacagag taagtgaagt cttcacccag cttgtattcg cccggaacat
    20461 aagtcggtgc gccagccgga ttgatttttt ctttaatgat ggcgtcaatg aagttacggc
    20521 tcatcaggtc acccagaacg tcctggcgta cagacgcgcc ataacgctga gcaacgatat
    20581 tcattggcac tttgcctttg cggaagccgt caatacgtac ttttttcgca acgttgacca
    20641 gctcgctttt aacagcggtc tcgatgctgt cagcagcgat agtaatcgtt acacggcggc
    20701 caaggccttg agtggtttca actgaaactt gcatcttgtt acctcaaaaa atcacagtgc
    20761 tcggtcaact cttaccccgc aagcacaggt tgttggcttc gcggaaccgg gatgttctta
    20821 taatcaatca cattccctgt taccagaatc atcccgaaag cattcaaata ggacgcggca
    20881 ttatagcggc atcactttta tgagtcgaga acggttattg cgcgctgtcg cgtatttttt
    20941 cacaattccc gggcaatttt cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa
    21001 caaaacggcc cgcaggccgt ttttcataaa atctttagca acatactgga aaagcgccga
    21061 cagttgcaaa tgcgttttta cgcgatgctt cctgctccac gacagggagg agaggcaaag
    21121 acggtgtcct gcaacccttc ccactcctta atagtgtaag tatgcagagc cagcgcatga
    21181 acggtagtag agagttcctc cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa
    21241 cgttcacccg taaaacgatc gctgaccagc acaactttaa aatggctttc agagccggct
    21301 gggacattgt gacgatagct ttcatccact acttcgagga atacgggttg gaacgccgcc
    21361 cttaattttt cttctatccg ctcacgtatc atcatgaaat atctcctccg acaacgctga
    21421 gatgtcatcc atccctttaa atactagccg cttttaccgt ttccattgca gcttaacaac
    21481 aaatatttag gtttcgtcct ggtttttccg tcaagcgtat gaagtttact gatttaaaga
    21541 ttgaaacaca ggcctcgcat cagcgtttct tttaccaggg gtgattattt cgtcaacatg
    21601 atgaatttac atgccttacc cacttcccct cgccgttggc aatgttatga tggcgggaat
    21661 ttttaccaaa cacgcttaag agtctctgag agcccgaaca tgttcaaaaa aatcctcttc
    21721 ccgttagttg ctctgtttat gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt
    21781 tccccgacga ttacactgcc acagcaggat ccaagcctga tgggcgtcac cgtaagcatt
    21841 aatggtgccg atcagcgtac agatcaggcg ctggcaaaag tcacccgcga taatcaaatc
    21901 gttaccctga ccgcctcccg cgatctgcgt ttcctgctgc aagaagtgct ggaaaaacag
    21961 atgaccgcgc gtggttacat ggttggtccg aatggcccgg ttaatctgca aatcatcgtt
    22021 agccaactgt atgctgacgt gtcccagggc aacgtgcgct acaacatcgc taccaaagcg
    22081 gatatcgcca tcatcgctac cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc
    22141 agctacaacg ttgaaggtgc gttccaggcc tccaacaaaa atatcgccga tgcggttaac
    22201 agtgtgctga ccgataccat cgctgatatg tctcaggaca ccagcattca cgaattcatc
    22261 aagcagaacg cgcgttaatt tctgccctct ggcccggtgc aagccgggcc tgtagacgcc
    22321 catgtccagt caatatttac gtatttttca acagccgcgt tcagccatat tgctgatcct
    22381 gggttttgct tccgggctac cgctcgccct gacatccggc accttacagg cctggatgac
    22441 ggtcgaaaat atcgatctca aaaccattgg tttcttctct ctggtaggcc aggcttacgt
    22501 ttttaaattc ctctggtcac cgctgatgga ccgctacacg cccccatttt ttgggcggcg
    22561 gcgcggttgg ctgctcgcca cgcaaatcct gttattagtc gccattgcgg cgatgggttt
    22621 tctcgaacca ggcacccaac tccgctggat ggcggcgctg gcggtggtga tcgctttttg
    22681 ctctgcctcc caggatattg tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga
    22741 acgtggtgcg ggcgcggcaa tcagcgtgct gggttaccgt ttagggatgc tggtttccgg
    22801 cggcctagcc ctgtggctgg c
//
LOCUS       DAJTYI010000056        22496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYI010000056 DAJTYI010000000
VERSION     DAJTYI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..509
                     /locus_tag="OO564_002105"
     CDS             <1..509
                     /locus_tag="OO564_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6653233.1"
                     /translation="SCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLST
                     GAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRM
                     WLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQ
                     KTPVHNTR"
     gene            530..1188
                     /gene="csiR"
                     /locus_tag="OO564_002106"
                     /pseudo
     CDS             530..1188
                     /gene="csiR"
                     /locus_tag="OO564_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1189..1638)
                     /gene="kbp"
                     /locus_tag="OO564_002107"
     CDS             complement(1189..1638)
                     /gene="kbp"
                     /locus_tag="OO564_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6653234.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1722..1880)
                     /locus_tag="OO564_002108"
     CDS             complement(1722..1880)
                     /locus_tag="OO564_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6653235.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2063..2362
                     /locus_tag="OO564_002109"
     CDS             2063..2362
                     /locus_tag="OO564_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6653236.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2372..2896
                     /gene="ygaP"
                     /locus_tag="OO564_002110"
     CDS             2372..2896
                     /gene="ygaP"
                     /locus_tag="OO564_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6653237.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(2943..3347)
                     /gene="stpA"
                     /locus_tag="OO564_002111"
     CDS             complement(2943..3347)
                     /gene="stpA"
                     /locus_tag="OO564_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6653238.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4015..4452
                     /gene="alaE"
                     /locus_tag="OO564_002112"
     CDS             4015..4452
                     /gene="alaE"
                     /locus_tag="OO564_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6653239.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4489..4839)
                     /locus_tag="OO564_002113"
     CDS             complement(4489..4839)
                     /locus_tag="OO564_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6653240.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            4991..5320
                     /locus_tag="OO564_002114"
     CDS             4991..5320
                     /locus_tag="OO564_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6653241.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            5568..5813
                     /gene="nrdH"
                     /locus_tag="OO564_002115"
     CDS             5568..5813
                     /gene="nrdH"
                     /locus_tag="OO564_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6653242.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5810..6220
                     /gene="nrdI"
                     /locus_tag="OO564_002116"
     CDS             5810..6220
                     /gene="nrdI"
                     /locus_tag="OO564_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6653243.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6193..8337
                     /gene="nrdE"
                     /locus_tag="OO564_002117"
     CDS             6193..8337
                     /gene="nrdE"
                     /locus_tag="OO564_002117"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6653244.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8347..9306
                     /gene="nrdF"
                     /locus_tag="OO564_002118"
     CDS             8347..9306
                     /gene="nrdF"
                     /locus_tag="OO564_002118"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6653245.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9661..10863
                     /gene="proV"
                     /locus_tag="OO564_002119"
     CDS             9661..10863
                     /gene="proV"
                     /locus_tag="OO564_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6653246.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10856..11920
                     /gene="proW"
                     /locus_tag="OO564_002120"
     CDS             10856..11920
                     /gene="proW"
                     /locus_tag="OO564_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6653247.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            11978..12970
                     /gene="proX"
                     /locus_tag="OO564_002121"
     CDS             11978..12970
                     /gene="proX"
                     /locus_tag="OO564_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6653248.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13162..14341
                     /locus_tag="OO564_002122"
                     /pseudo
     CDS             13162..14341
                     /locus_tag="OO564_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14465..15202
                     /gene="ygaZ"
                     /locus_tag="OO564_002123"
     CDS             14465..15202
                     /gene="ygaZ"
                     /locus_tag="OO564_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6653249.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15192..15527
                     /gene="ygaH"
                     /locus_tag="OO564_002124"
     CDS             15192..15527
                     /gene="ygaH"
                     /locus_tag="OO564_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6653250.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15618..16148
                     /gene="emrR"
                     /locus_tag="OO564_002125"
     CDS             15618..16148
                     /gene="emrR"
                     /locus_tag="OO564_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6653251.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16275..17447
                     /gene="emrA"
                     /locus_tag="OO564_002126"
                     /pseudo
     CDS             16275..17447
                     /gene="emrA"
                     /locus_tag="OO564_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17464..19002
                     /gene="emrB"
                     /locus_tag="OO564_002127"
     CDS             17464..19002
                     /gene="emrB"
                     /locus_tag="OO564_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6653252.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19066..19581)
                     /gene="luxS"
                     /locus_tag="OO564_002128"
     CDS             complement(19066..19581)
                     /gene="luxS"
                     /locus_tag="OO564_002128"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6653253.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19731..21287)
                     /gene="gshA"
                     /locus_tag="OO564_002129"
     CDS             complement(19731..21287)
                     /gene="gshA"
                     /locus_tag="OO564_002129"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6653254.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21360..21788)
                     /locus_tag="OO564_002130"
                     /pseudo
     CDS             complement(21360..21788)
                     /locus_tag="OO564_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21785..22351)
                     /gene="yqaB"
                     /locus_tag="OO564_002131"
     CDS             complement(21785..22351)
                     /gene="yqaB"
                     /locus_tag="OO564_002131"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6653255.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5412 a         5736 c         5640 g         5708 t
ORIGIN      
        1 ccagttgcct gaactcggcc ctgtataccg cctcaaggat gctctactcc ttaagccgtc
       61 gcggtgatgc tcccgcggta atgggtaaaa tcaaccgcag taaaaccccg tacgtggcgg
      121 tgttactctc caccggcgca gcattcttaa cggtggtggt gaactattac gcgcctgcga
      181 aggtatttaa atttctgatc gacagctccg gcgctatcgc cctgctggtt tatttagtca
      241 tcgccgtttc acagttgcgg atgcgcaaaa ttctgcgagc agaaggaagc gaaattcgct
      301 tgcgaatgtg gctttatccc tggctcacct ggctggtcat cggctttatt acctttgtgt
      361 tggtagtgat gctattccgt ccggcgcaac agttagaagt gatctccacc ggcttattag
      421 cgatagggat tatctgtacc gtgccaatta tggctcgctg gaaaaagctg gtattgtggc
      481 aaaaaacacc cgttcataat acgcgctgat aatgatcagg agtcacacca tgaccattac
      541 gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga
      601 tgaaaaatta cggatgagtt tactgacatc gcgttatgca cttggcgttg ggccgttgcg
      661 ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta
      721 tcgggtggcg tctatgtcag agcaggagct gctctgacat agacgccacc cgatatggaa
      781 gcgatgctgg tcagtctggc gattgcccgc ggtggcgatg aatgggaggc agacgttctc
      841 gcaaaagcgc atctgctcaa taagctggaa gcctgtgacg ccagcgagaa aatgcttgat
      901 gagtgggatc tgcgtcatca ggcgtttcat acagcaattg tggcgggctg tggttctcac
      961 tatttgctgc aaatgcgtga acggttgttt gatctggcgg cgcgttatcg atttatctgg
     1021 ctgcggcgaa cggtgctttc ggtggaaatg ctggaggata aacacgatca gcaccagacc
     1081 ctgactgcgg cggtactggc gcgagataca gcgcgcgtca gtgagttaat gcgccagcat
     1141 ttactgacgc caattcccat tatccagcag gcgatggctg gcaattaact actcttccgg
     1201 aatacgcaac acttgccccg gataaatttt atccgggctt tttagcatcg gtttattcgc
     1261 ttcgaagatt ttattgtaca gattagcgtt accgtagacc tgtttggaaa tggcactcag
     1321 agtgtcgcca gacttaacgg tataaaactg gctggcagtg gctggtgtcg ccgttttcac
     1381 ctgatcatca acactggcaa taccggaaat attccccacc gcaacaagga ttttctcctt
     1441 cgcctcctga ctcaggccgt caccagtgac cgtcgctttg ccgtcggcaa tttgaatgtt
     1501 cactttatcg gcatcaggta taccggtttt gctcagatgc tcctgcacct tcttcgcctg
     1561 atcgtcttta tcgtgctgac ctgtaaccgc atcccagagt ttttctccgg catctttcac
     1621 aaaattgaac agacccatag ctacctcatt cgttaacgga aacactcaga aagtgtagca
     1681 gaggaacgcg gcgcgttaac gccgcggggg tgagcacagg tttaatcgcg ggtttgcacc
     1741 cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg tcagcagaat attaataatg
     1801 aacgcccaac cgaacccttt accgagcagc acgccgagcg gcggcagaat gatggtgatg
     1861 acgattctcc agaaacccat atgtactccc tataagaaaa ttactcattg tttaaaaaga
     1921 gattttatct cttaagcgta gtaattttag cggagcctgc cagtttcagc ttacgctttt
     1981 actctcattt acgctgctga ggctggtata tgttgcattt ttagtttaga gtttacttaa
     2041 tttagaaaat acttaaataa atatgactga actcgcgcaa ttacaggcca gtaccgaaca
     2101 ggcagcggcc ttattgaaag caatgagcca ccctaaacgg ttgctgattc tgtgcatgct
     2161 tagcggttcc cccggcacca gcgcgggaga gctgacgcgc attaccggac tgagcgcctc
     2221 tgcgacatca cagcatctcg ctcgtatgcg ggacgaaggg cttatcaaca gccaacggga
     2281 tgcccaacgc attctatatt ccattaaaaa tgaggcggta aatgccatta tcgccaccct
     2341 gaaaaatgtc tattgtccgt aaggagtcac tatggctttg acaaccattt cgccgcatga
     2401 tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat attcgtgacg ctgatgaata
     2461 tcttcgtgaa catattcctg aagcagatct ggctccatta tccgtgctgg aacagtcagg
     2521 tcttccacct aaattacgtc gcgagcaaat tatattccac tgccaggcag gtaaacgcac
     2581 cagtaataac gccgataaat tagcagcgat tgccgccccc gcagaaatat ttttactcga
     2641 agatgggatt gatggctgga aaagagctgg attgccagta gcggtaaata aatctcaacc
     2701 cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt ttaatattaa tcggcgttgt
     2761 actgggttat accgtaaata gcggtttctt cttattaagt ggctttgttg gtgccgggtt
     2821 actgtttgca ggaatcagcg gtttttgtgg aatggcaagg ttgttagata agataccgtg
     2881 gaaccaacga gcttgagaag cgacgccgga cgcgccctag cagcgacatc cggcctcagt
     2941 aattagatca ggaaatcgtc gagagattta ccttctgcca gcgcctgagc aattggcttc
     3001 ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt taacatcagt gaatttatat
     3061 ttcgccggac gcggctggcg ttttttacca gcacgtggtg cagcagcaga gctattaccc
     3121 aataactctt ccgggttaat tccgtcagct ttcatcagct ccagccaggt gctaattttt
     3181 tcctgacgct cagccagttc acgctgctgc tgttcttctt cttcacgtct ttctttagtg
     3241 acaaccctga atttttcgag catttcttca agaacgtcaa tggagaattc gcgagccatc
     3301 gcacggaggg tgcgaatgtt atttaaactt tgtaacatta cggacataat aaagaaaacc
     3361 ttttaacgcc aaaacaaaaa agtatttcgt cgcgtagtgt aagcgatcac ttattatatt
     3421 tccacaaaat ttaatctatt tacagtcctg cgcgagatta tttcagctgt tattttctga
     3481 aactctcaga ataatttcaa gattatatat tagccatttt gatgagtatt ttaggttatt
     3541 ggtgaacaga gtggcgctct ctgctcgcta actccgtaag cctgacaaaa ccgaagtgaa
     3601 taaaaatgca atataacaga ttaaaagaaa acacccctca ctggtgcttt ctgctgttaa
     3661 tgtacatttt ctggcatcaa aaatgctgta aatgacccgg tcacgacaaa caccgaattc
     3721 tctacgctta tagcgttacc tcccccccaa actttcatta gtgaaattaa aggcaaggcc
     3781 atatccaatt gatttaattg aaaataaatc atatcggcat gtaaaaatcc acaatgtaca
     3841 aaaaacgagc cgttacggaa tattttatct acaaaagctg actaaataaa aatttttcac
     3901 taatttatta gtcatagcca gcgatatacg ctatgcgaaa atacagatgg caatgagatc
     3961 cactgctttc atctccatta acatcccatt acgcttttat taaggagcat tagcatgttc
     4021 tcaccgcagt cacgcttgcg tcatgcagtt gcagatacgt tcgcgatggt tgtttactgt
     4081 tctgtcgtga acatgtgtat tgaagttttc ctctccggaa tgagcttcga acagtctttt
     4141 tattccagat tggtagcgat tccggtgaac atcttaattg catggccata cggtatgtac
     4201 cgcgatctgt ttatgcgcgc ggcacgcaaa ggctggataa aaaatctggc tgatatcctg
     4261 gcttatgtga cgttccagtc accggtgtat gtggcgatct tgttagtggt gggcgcagac
     4321 tggcatcaga ttatggcggc ggtcagttca aacatcgttg tttcgatgtt gatgggggcg
     4381 gtttatggct acttcctcga ttattgccgc cgactgttta aagtcagccg ttaccagcag
     4441 gtaaaagcct gactcttctt ttcgcgactg gcatcgccag tcgcgtcttt aactggcttc
     4501 gccaaataaa ccattcaaat aacgttcaag cgcaacacgc gaactaaagc catgcgggat
     4561 accgtaatgc tcgtgtcgct gtcgctcgcc tgctaaatag agcggaaatt gctggtaatg
     4621 atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt gaactgcgtt cttttaatgt
     4681 tggatgaatt accagagcgg tacgtcccat tcgcgcttcg cgattaacat agacataatt
     4741 ttccccacgg cggtaaccat acgctttctg ggttaccaca tcgacagtaa aaccgacttt
     4801 ttcaagtacg cgcgccacct cgtctggtcg taaatacata tttgatcctc gttattttca
     4861 accgcgggcc taccttacct gattgcgcat tcacaacgct ttcagaaaag tccataaagc
     4921 cgcgatgaac ctcagttaag aaaattatgg tctacactga aaattacatc gaattctaat
     4981 gggggatcat atgtttaacc gaccgaaccg caacgacgtt gatgatggcg tgcaggatat
     5041 ccagaatgat gtcaatcaat tagctgacag cctcgaatct gtattgaagt cctggggcag
     5101 cgacgccaaa ggggaagctg aagccgcacg cagtaaagct caggcgttgc tgaaagaaac
     5161 cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc gcgcgcgatg ccgttggctg
     5221 cgcggattct tttgttcgtg aaagaccctg gtgtagcgtg ggtacagcag ctgcggtagg
     5281 tatttttatc ggcgcactgt taagcatgcg caaatcgtag tgcaaaaatg ataataaata
     5341 cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt tgcctggtga atcacaaaaa
     5401 tccccctccc ccgtcacgct catatccagg gtaatttcga tcactatttg ctatatattg
     5461 tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag agacaacccg
     5521 acgcgtatca tcgcgccgta tcttcatttt aaacagaaat acgaatcatg cgcataacta
     5581 tttatactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg gaaaaccggg
     5641 gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcagcggaa gcgttgcgtg
     5701 ctcagggctt tcgtcagttg ccggtagtga ttgctggcga tcttagctgg tctggtttcc
     5761 gtccggacat gattaaccgt ctgcatccag cgccacacgc ggccagtgca tgagccagct
     5821 cgtctacttc tccagcagct ccgaaaacac gcagcgtttt atcgaacgtt taggtctgcc
     5881 cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc cttacatcct
     5941 gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac aggtaattcg
     6001 ctttttaaac gacgagcaca accgggcgtt gcttcgcggc gttattgctt ctggtaatcg
     6061 caactttggt gaggcgtatg gccgcgccgg agatgtgatt gcccggaaat gcggcgtgcc
     6121 gtggctgtac cgttttgaac tcatgggtac gcaaagcgat atcgaaaacg ttcgtaaagg
     6181 agtaaccgaa ttttggcaac gacaaccgca gaatgcctga cgcaggaaac gatggattac
     6241 cacgcgctga atgcgatgct taatctttac gatagcgcag gtcgcattca gttcgataaa
     6301 gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc gtccgaacag tgtgaccttc
     6361 agtagccagc agcagcgcct gaactggctg gtcaacgaag gttactatga tgaaagcgtt
     6421 cttaatcgct actctcgcga ctttgtcatt acgctgtttg cccacgcaca caccagcggt
     6481 tttcgtttcc agacattcct cggggcatgg aagttttaca ccagctatac gttgaagaca
     6541 ttcgacggta aacgttatct ggaagatttt gccgatcgag tcacgatggt ggcgctgacg
     6601 ctggcacaag gcgatgagac gctggcgttg caactgaccg atgaaatgct gtcaggacgc
     6661 tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg cgaactggtt
     6721 tcctgttttt tgctgcgtat tgaagacaat atggagtcga ttggtcgggc ggtaaattcc
     6781 gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa tctgcgggaa
     6841 gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtaattcc ggtgatgaaa
     6901 atgttggaag acgcattttc ctatgccaac caactcggcg cgcgtcaggg ggcaggtgca
     6961 gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgacacgaa acgggaaaat
     7021 gccgacgaaa aaatccgcat taaaacactg tcgctgggcg tggtgatccc ggatatcact
     7081 ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt cgccctatga cgttgagcga
     7141 gtttatggca agccgtttgc cgatatcgcc attagcgaac actatgacga actggttgcc
     7201 gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt tcttccagcg actggcagaa
     7261 atccagtttg agtccggcta tccctacatc atgtatgaag acacagtaaa ccgtgctaac
     7321 cctatcgccg ggcgcataaa tatgagtaat ctctgctcag aaattttgca ggttaacagc
     7381 gcctcagagt atgacgagaa tctcgactat gcccgcacag gccatgatat ttcctgcaat
     7441 ttaggttcgt tgaatattgc gcacaccatg gattcccccg atttttcccg cacggtagag
     7501 actgccgtgc gcggtttaac ggcggtgtca gatatgagtc atatccgcag cgtgccgtcc
     7561 atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg ggcagatgaa tttacatggt
     7621 tatctggcga gggaaggcat cgcttatggt tcgccggaag ccctggattt caccaatctc
     7681 tatttctata ccatcacctg gcacgcactg cgtacctcga tgttgctggc gcgcgaacgc
     7741 ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtggtgaata tttcagccaa
     7801 tatctgcaag gtaactggca gccgaaaacg gcgaaagttg gcgaactgtt tgcccgcagc
     7861 ggtattacgt tacctacccg tgagatgtgg gcgcaactgc gcgacgacgt gatgcgctac
     7921 ggcatataca accagaatct ccaggcggtg ccgccaaccg ggtctatctc ttatatcaac
     7981 catgctacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa agagggcaaa
     8041 acaggacgcg tttactaccc tgccccgttt atgactaacg aaaatctggc gctgtatcag
     8101 gacgcttacg aaattggcgc agaaaagatc atcgacacct acgcggaagc gactcgccat
     8161 gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac tcgcgatatc
     8221 aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa cgctctatta catccgcctg
     8281 cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc actttaagga
     8341 atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc tgacgataaa
     8401 gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa ggtgccgctg
     8461 tcgaacgata ttcccgcctg gcagacatta actgtcgtag aacaacaact gacgatgcgc
     8521 gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc gccttcgctg
     8581 atgcccgatg cgctcacgcc tcatgaagag gcggtattat cgaatatcag ctttatggaa
     8641 gcggttcatg cccgctctta cagttcgatt ttctcgacgc tgtgccagac caaagatgtc
     8701 gatgccgcct acgcctggag tgaagaaaac gcaccgttgc agcgaaaagc tcagattatt
     8761 cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt gtttcttgaa
     8821 tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg cggaaagctg
     8881 accaataccg cggacctgat ccgtctgatt atccgcgatg aagcagtcca cggttactac
     8941 ataggctata aatatcagaa aaacatggaa aagatatctc tgggacaacg tgaagagttg
     9001 aagagtttcg ccttcgattt gttgctggaa ctctacgaca acgagttgca atacaccgat
     9061 gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag cgtttctctg ttacaacacc
     9121 aataaggctt tgatgaatct gggctacgaa ccgttatttc ccgcagaaat ggcggaagtg
     9181 aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga tttcttttcc
     9241 ggttcaggct cctcttatgt gatggggaaa gcggttgaaa cagaagatga agactggaat
     9301 ttctgagggt gttattttca aaaatatcac tacccgcagc agggaaataa ttcccgccaa
     9361 atagcttttt atcacgcaaa tattctgtag tgatctacac tgatactctg ttgcattatt
     9421 cgcctgaaac tacaatattc aggcgttttt tcgctatcta tgacaaaaaa tatcaacttt
     9481 tctcgatttg ctctcagccc ttatatcacg gaaaattccg gcgatttgct cgcatcaata
     9541 ttcatgccac atttgccctc aggggttgcc tcagattctc agtatgttag ggtagaaaaa
     9601 agtgactatt cccattgggt aatatatcga cataaacaaa taaaggaatc attctattgc
     9661 atggccatta aattagaaat taaaaatctt tataaaatat ttggtgagca tccacagcga
     9721 gcgttcaaat atatcgaaca aggactttca aaagaacaaa ttctggaaaa aactgggcta
     9781 tcgcttggcg taaaagacgc cagtctggcc attgaagaag gcgagatatt tgtcatcatg
     9841 ggattatccg gctcgggtaa atccacaatg gtacgccttc tcaatcgcct gattgaaccc
     9901 acccgcgggc aagtgctgat tgatggtgtg gatattgcca aaatatccga cgccgaactc
     9961 cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt cctttgcctt aatgccgcat
    10021 atgaccgtgc tggacaatac tgcgttcggt atggaattgg ccggaattaa tgccgaagaa
    10081 cgccgggaaa aagcccttga tgcactgcgt caggtcgggc tggaaaatta tgcccacagc
    10141 tacccggatg aactctctgg cgggatgcgt caacgtgtgg gattagcccg cgcgttagcg
    10201 attaatccgg atatattatt aatggacgaa gccttctcgg cgctcgatcc attaattcgc
    10261 accgagatgc aggatgagct ggtaaaatta caggcgaaac atcagcgcac cattgtcttt
    10321 atttcccacg atcttgatga agccatgcgt attggcgacc gaattgccat tatgcaaaat
    10381 ggtgaagtgg tacaggtcgg cacaccggat gaaattctca ataatccggc gaatgattat
    10441 gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt tcagtgcgaa agatattgcc
    10501 cgccggacac cgaatggctt aattcgtaaa actcctggct tcggcccacg ttcggcactg
    10561 aaattattgc aggatgaaga tcgcgaatat ggctacgtta tcgaacgcgg taataagttt
    10621 gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa cgcagcagca aggtcttgat
    10681 gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa cgcctcttag cgagttgctc
    10741 tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg acgaggacca acagtatgtc
    10801 ggcatcattt cgaaaggaat gctgctgcgc gctttagatc gtgagggggt aaataatggc
    10861 tgatcaaaat aatccgtggg ataccacgcc agcggcggac agtgccgcgc aatccgcaga
    10921 cgcctggggg acaccgacga ctgcaccggc tgacggcggt ggtgctgact ggctgaccag
    10981 tacgcctgcg ccaaacgtcg agcattttaa tattctcgat ccgttccata aaacgctgat
    11041 cccgctcgac agttgggtca ctgaagggat cgactgggtt gttacccatt tccgtcccgt
    11101 cttccagggc gtgcgcgttc cggttgatta tatcctcaac ggtttccagc aattgctgct
    11161 gggtatgcca gcgccagtgg cgattatcgt tttcgctctc atcgcctggc agatttccgg
    11221 ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc atcggcgcaa tcggtgcctg
    11281 ttcgcaggca atggtgactc tggcgctggt gttaactgcc ctgctgttct gtatcgtcat
    11341 cggtttgccg ttggggatat ggctggcgag aagtccgcga gcggcgaaaa ttattcgtcc
    11401 actgcttgat gccatgcaga ccacgccagc gtttgtttat ctggtgccaa tcgtcatgct
    11461 atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc atctttgcgc tgccgccgat
    11521 tatccgtctg accattctgg ggattaacca ggttccggcg gatctgattg aagcctcgcg
    11581 ctcattcggt gccagcccgc gccagatgct gttcaaagtt cagttaccgc tggcgatgcc
    11641 gaccattatg gcgggcgtta accagacgct gatgctggcc ctttctatgg tggtcattgc
    11701 ctcgatgatt gccgtcggcg ggctgggtca gatggtactt cgcggtatcg gtcgtctgga
    11761 tatggggctt gccaccgttg gcggcgtcgg gattgtgatc ctcgccatta tcctcgatcg
    11821 cctgacgcag gccgttgggc gcgactcacg cagtcgcggc aaccgtcgct ggtacaccac
    11881 tggccctgtt ggtctgctga cccgcccatt cattaagtaa ctctgcactt gcccggtgac
    11941 gccgggcatt atcaccctgc caaaaaaagg aataacaatg cgacatagcg tactttttgc
    12001 gacagcgttt gccacgctta tctctacaca aacttttgct gccgatctgc cgggcaaagg
    12061 cattactgtt aatccagttc agagcaccat cactgaagaa accttccaga cgctgctggt
    12121 cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa cccagcgaag tggattacaa
    12181 cgttggctac acctcgcttg cttccggcga tgcaaccttc accgccgtga actggacgcc
    12241 actccatgac aacatgtacg aagctgccgg tggcgataag aaattttatc gtgaaggggt
    12301 atttgttaac ggcgcagcac agggttacct gatcgataag aaaaccgccg accaatacaa
    12361 aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc aaactgttcg ataccaacgg
    12421 cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg ggctgcgaag gtgcgatcaa
    12481 ccaccagctt gccgcgtatg aactgaccaa caccgtgacg cataatcagg ggaactacgc
    12541 agcgatgatg gccgacacca tcagtcgcta caaagagggc aaaccggtgt tttattacac
    12601 ctggacgccg tactgggtga gtaatgagct gaagccaggg aaagatgtgg tctggttgca
    12661 ggtgccgttc tccgcactgc cgggcgataa aaatgccgat accaaactgc cgaatggcgc
    12721 gaattacggc ttcccggtca gcaccatgca tatcgttgcc aacaaagcct ggaccgagaa
    12781 aaatccggcg gcagccaaac tgtttgccat tatgcagttg ccagtggccg atattaacgc
    12841 ccagaacgcc attatgcatg acggcaaagc ctcagaaggc gatattcagg gccacgttga
    12901 tggctggatc aaagcccacc agcagcagtt cgatggctgg gtgaatgagg cgctggcagc
    12961 gcagaagtaa tttttattcg ggcggataag gcgttttcgc ctcatccgcc gctctgtaca
    13021 caatgcctga tgcgacgctg acgcgtccta tcatgcctac aaaccacatc gcacatttca
    13081 acaaactatc tttcatccca tattcatcaa catccgctat tattgatttc cagcttaatc
    13141 atcacctgat gaacaaaaat aatgactaaa cctaatcatg agcttagccc ggcgctgatc
    13201 gtgctgatgt ctatcgccac cggtctggcg gtagccagta actattacgc ccagccattg
    13261 ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt tattgttacc
    13321 gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg ttcccctcgg tgatatgttt
    13381 gaacgccgcc gcctgattgt ctcgatgacc ttactggcag cgggcggtat gttaatcact
    13441 gccagcagtc agtcactggc gatgatgatc ctcggtacgg cattaaccgg tttattctca
    13501 gtcgtggcac aaattctggt tccgctggca gcgacgctgg cttcaccgga taaacgcggc
    13561 aaagtggttg gcaccattat gagcggtctg ctgttgggga tattgctggc acgaaccgtt
    13621 gccgggttac tggcaaacct cggcggctgg cgcaccgtct tttgggttgc ttcggtgtta
    13681 atggcactga tggcgctggc attatggcgt ggtctgccac aaatgaaatc agaaacccac
    13741 ctcaactacc cacagttgtt gggttccgtt ttcagcatgt tcatcagcga taagatcctg
    13801 cgcacccgcg cgttgctggg ctgcatgacc tttgccaact tcagcattct ctggacctca
    13861 atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgacat tggtctgttt
    13921 gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt tgccgacaag
    13981 ggcaaatcgc accacaactt tcggtctgct tctgctgtta ctttcatggc tggcaatctg
    14041 gtttggtcac acttccgtac tggcgttgat tatcggcatt ctggtgctgg acctcaccgt
    14101 acagggcgtg catatcacta accagacggt aatttatcga atacatcctg atgcgcgtaa
    14161 tcgcctgacc gcaggttaca tgaccagcta ctttattggc ggtgccgccg gttcgctaat
    14221 ttcagcctca gcctggcaac atggcggttg ggctggcgtt tgtctggctg gcgcgacgat
    14281 tgccctggtt aacttactgg tctggtggcg aggttttcat cgtcaggaag ccgcaaatta
    14341 agcaatcagc aaccgttttc gtggctttac acttataagg gtgttaagaa gcccatcagt
    14401 ctgataaggt taagatattc attcagtcta tttataatat taacaatcgt taagcgtaca
    14461 ctctatggaa agccctactc cacagcctgc tcctggttcg gcgaccttca tggaaggatg
    14521 caaagacagt ttaccgattg ttattagtta tattccggtg gcctttgcgt tcggtctgaa
    14581 tgcgacccgt ctgggattct ctcctctcga aagcgttttt ttctcctgca tcatttatgc
    14641 aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc gggagtagtt tgtgggttgc
    14701 tgcactgacc gtcatggcaa tggatgttcg ccatgttttg tatggcccgt cactgcgtag
    14761 ccgtattatt cagcgtctgc aaaaatcgaa aactgcccta tgggcgtttg gcctgacgga
    14821 tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa
    14881 ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg gtatttggta cggtaatagg
    14941 ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc gttgaagctg cattaggttt
    15001 tatgcttccg gcactcttta tgagtttcct gctcgcctct ttccagcgca aacaatctct
    15061 ttgcgttacc gcagctttag ttggtgccct tgcaggcgta acgctatttt ctattcccgt
    15121 cgccattctg gcaggcattg tctgtggctg cctcactgcg ttaatccagg cattctggca
    15181 aggagcgccc gatgagctat gaggttctgc tgcttgggtt actggttggc gcggcgaatt
    15241 attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg
    15301 gcgcggtagg tattttgctc gacaccattg gcatcgcctc gatatgcgct ctgctggttg
    15361 tctctaccgc accagaagtg atgcacgata cacgccgttt cgtgcccacg ctggtcggct
    15421 tcgcggtact gggtgccagt ttctataaaa cacgcagcat tatcatccca acactgctta
    15481 gtgcgctggc ctatgggctc gcctggaaag tgatggcgat tatataaccc acaagaatca
    15541 tttttctaaa acaatacatt tactttattt gtcactgtcg ttactatatc ggctgaaatt
    15601 aatgaggtca tacccaaatg gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc
    15661 gcgccagccg ccacgaagat tttccttatc aggagatcct tctgactcgt ctttgcatgc
    15721 acatgcaaag caagctgctg gagaaccgca ataaaatgct gaaggctcag gggattaacg
    15781 agacgttgtt tatggcgttg attacgctgg agtctcagga aaaccacagt attcagcctt
    15841 ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac
    15901 tggaaaaacg cggttggatc gaacgtcgtg aaagcgataa cgatcgccgc tgcctgcatc
    15961 tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg cagcataact
    16021 gcctgcatca actctggtcc gcgctcagca caacagaaaa agatcagctc gagcaaatca
    16081 cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga
    16141 tgagctaacg cgtcatctcg ctcaaaaatc cagatttata aaagaaaaaa tgaccggcca
    16201 gcatcgcaac atgctggcct ttttggcaag caggtcggct cagccgatga gttaagaaga
    16261 tcgtggagaa caatatgagc gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga
    16321 agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt gctctttata attattgccg
    16381 tagcgatagg gatttattgg tttttggtac tgcgtcattt cgaagaaacc gatgacgcat
    16441 acgtggcagg gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct
    16501 gggccgataa caccgatttt gtaaaagaag gcgatgttct ggtcactctc gacccgacag
    16561 atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc
    16621 accagctgat gattaacagc aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc
    16681 tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg
    16741 gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg caactggacg
    16801 tcgctattca acaatacaat gccaatcagg cgatgattct ggggactaaa ctggaagatc
    16861 agcctgccgt gcaacaggct gccaccgaag tacgtaacgc ctagctggcg ctggagcgta
    16921 cccgtattgt cagtccgatg accggttatg tttcccgtcg tgccgtgcaa ccgggtgcgc
    16981 aaattagccc aacgacgccg ctgatggcgg tcgttccagc caccaatatg tgggtggatg
    17041 ccaactttaa agagacgcag attgccaata tgcgcatcgg tcagccggtc accatcacca
    17101 ccgatattta cggcgatgat gtgaaataca ccggtaaagt ggttggtctg gatatgggca
    17161 caggtagcgc gttctcactg cttccggcgc aaaatgcgac cggtaactgg atcaaagtcg
    17221 ttcagcgtct acctgtgcgt atcgaactgg accagaaaca gctggagcaa tacccgctgc
    17281 gcattggttt gtccacgctg gtgagcgtca ataccactaa ccgtgacggt caggtactgg
    17341 caaataaagt acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc agcctagcac
    17401 ctgtcaataa actgatcgac gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt
    17461 gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg tcattatgac gattgcgctg
    17521 tcactggcga cattcatgca ggtgctggac tccaccattg ctaacgtggc gatccccact
    17581 atcgccggga atctgggctc atcgctcagc caaggaacgt gggtaatcac ttctttcggg
    17641 gtggcgaatg ccatctcgat cccgcttacc ggctggctgg caaagcgcgt cggggaagtg
    17701 aaactgttcc tttggtccac catcgccttt gctattgcgt cgtgggcgtg tggtgtctcc
    17761 agcagcctga atatgctgat cttcttccgc gtgattcagg ggattgtcgc cgggccgttg
    17821 atcccgcttt cgcaaagtct attgctgaat aactacccgc cagccaaacg ctcgatcgcg
    17881 ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat cctcggcggt
    17941 tatatcagcg ataattacca ctggggctgg atattcttca tcaacgtgcc gattggcgtg
    18001 gcggtggtgt tgatgacact gcaaactctg cgcggacgtg aaacccgcac cgaacggcgg
    18061 cggattgatg ccgtggggct ggcactgctg gttattggta tcggcagcct gcagattatg
    18121 ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg aaattatcat tcttaccgtg
    18181 gtggcggtgg tggctatctg cttcctgatt gtctgggagc tgaccgacga taacccgata
    18241 gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc
    18301 gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc agttgttgca ggaggtctac
    18361 ggttacacgg cgacctgggc aggtttggcc tctgcgccgg tagggattat tccggtgatt
    18421 ctgtcgccga ttatcggccg cttcgcgcat aaactggata tgcggcggct ggtaaccttc
    18481 agctttatta tgtatgccgt ctgcttctac tggcgtgcct atacctttga accaggtatg
    18541 gattttggcg cgtcggcctg gccgcagttt atccaggggt ttgcggtggc ctgcttcttt
    18601 atgccgctga ccaccattac gctgtctggt ttgccaccgg aacgactggc ggcggcatcg
    18661 agcctctcta actttacgcg aacgctggcg gggtctatcg gcacgtcgat aaccacgacc
    18721 atgtggacca accgcgagtc gatgcaccat gcgcagttga ctgagtcggt aaacccgttc
    18781 aacccgaatg cccaggcgat gtacagtcaa ctggaagggc ttgggatgac gcaacagcag
    18841 gcatcaggct ggattgccca gcagatcacc aatcaggggc tgattatttc cgccaatgag
    18901 atcttctgga tgtcagccgg gatattcctc gtactgctgg ggctggtgtg gtttgctaaa
    18961 ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact aagtacaact aagccagttc
    19021 atttgaactg gcttttttca attaattgtg aagatagttt actgactaga tgtgcagttc
    19081 ctgcaacttc tctttcggca gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc
    19141 cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca
    19201 ctggtagacg ttcagttccg ggatctgatt ctgatcctgc actttcagca cgtcttccat
    19261 tgccgctttc caggcatcag caacacgctg ctcatctggc gtaccaatca gactcatata
    19321 aaaaccggtg cggcagccca ttggcgagat atcgataatc tctacaccat taccgttaag
    19381 atggttacgc ataaaaccag caaacaggtg ctccagggta tggatccctc tttctggcat
    19441 cacttctttg ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg
    19501 ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc atccgggtat gatcgactgt
    19561 gaagctatct aacaacggca tttagccacc tccggtaatt tttttaaaaa ttttctgaac
    19621 tctttcttcc caggcgagtc tgagtatatg aaagacgcgc atttgttatc atcatccctg
    19681 aattcagaga tgaaattttg gccactcacg agtggccttt ttcttttctg tcaggcgtgt
    19741 ttttccagcc acaccgcaaa cggttcggta tcagcggttt ccatttcctg ctgacggcgt
    19801 tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca gaatttccag cggctcttca
    19861 cgcagcagat tacggtaggc ttctgcaaac gctttgcctg ttccgccaat accagtatca
    19921 atcatagacc ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc
    19981 agttcatcac acactttctg atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg
    20041 acgcgtttca gatcgtggaa cagatcttta cccacctgca gtaacgggaa ctgcgcggtt
    20101 tcgcagccga tacccagcgt caggcccggt ttgcgacctt caaggatcac ccggttccag
    20161 ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg gtgcatcagc cagcgcacac
    20221 cagaccataa acaggtcgag gaatcgcacc tgctgttcat ctacaccaat cggcgagaac
    20281 gggttgatgt ccagcgaacg cacttcaata tattcaatgc cgccacgtaa cagcgcatca
    20341 gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt
    20401 tcaatctgca gcacgttgct attgatttgc agcctcttac cgtctttctc aataccaatc
    20461 ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa
    20521 agatcgttga aggtaatacc aagattgctt tgcgatttat tggtatagcc gagatcgctc
    20581 aaacgaagag aggtcgcata cggcaggtaa tacataccgc actcggtttt ctcaaacggc
    20641 agcgacgttg gttttccttg caggaaagaa gaacaaatcg ccggagatgc accaaacaga
    20701 taaggaatga cccaaccgaa acgatagtaa ttgcggataa cgcggaaata gcccgcagaa
    20761 attttctctt tggcatcagc gcccgagata tcaccgcact tcgcttgcca gaatgccatt
    20821 ggcaaagaga aattgtagtg cacgccggaa atggtttgca tcagcgcgcc gtagcgattt
    20881 ttcagccctt cacgatacag cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc
    20941 agttcgatgt cctgaccttc tgcgatgtag catggcatac ttaacggcca catccgctca
    21001 tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca taaaggtcag catatgttca
    21061 atatcaccat ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc
    21121 catttgtgcg tcagtgcgga acctaatgct tcaggatgac ctgttgttgc cagtgtgcca
    21181 tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt taacgcctga
    21241 ggatgttttt ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc
    21301 ctgtcaaaat cgttttaatt agcataactg taatggtgac catatgtgca ggcctacaat
    21361 tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct
    21421 ttaccaaggc acaaaaaaaa gattactggc ccccaggaga tgcgcatcca tcccgctaac
    21481 agacacagta aatcgccaac cacgggcatc cagcttaata atagcgtgac tgcaccatag
    21541 cgtttcagcc agccggtagc tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa
    21601 cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc tgttaaaact
    21661 aaaacctagg gatgactgat cccggaaagc aacattgcca ccagcacgac ttcggagttg
    21721 ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag cgataacgct
    21781 tcactcacag caagcgaaca tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac
    21841 cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata cgctgcgcgc
    21901 acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg
    21961 cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc ctctgcgatg gcgctttcac
    22021 tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccact tcaacaagag
    22081 gaagcggctc gacactatcc agcagcatac ttcttactgc ttctgttttt tcacgcgcta
    22141 acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca atacgccagg
    22201 tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg tagtgcccta
    22261 atacttcgcg ccacgcttta cggtgcgtag gctccgtatc caggattgtg ccatccatat
    22321 caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg
    22381 ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa aattcaggaa
    22441 atggataaag tagttattta gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DAJTYI010000057        22403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYI010000057 DAJTYI010000000
VERSION     DAJTYI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1215
                     /gene="thrS"
                     /locus_tag="OO564_002132"
     CDS             <1..1215
                     /gene="thrS"
                     /locus_tag="OO564_002132"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6653256.1"
                     /translation="AAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELEVFVR
                     SKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQI
                     FNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVN
                     GCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQL
                     GEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPVMIHRAI
                     LGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKAD
                     LRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQ
                     EIRSRSLKQLEE"
     gene            1219..1761
                     /gene="infC"
                     /locus_tag="OO564_002133"
     CDS             1219..1761
                     /gene="infC"
                     /locus_tag="OO564_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6653257.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            1858..2055
                     /gene="rpmI"
                     /locus_tag="OO564_002134"
     CDS             1858..2055
                     /gene="rpmI"
                     /locus_tag="OO564_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6653258.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            2108..2464
                     /gene="rplT"
                     /locus_tag="OO564_002135"
     CDS             2108..2464
                     /gene="rplT"
                     /locus_tag="OO564_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6653259.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    2581..2705
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            2587..2631
                     /gene="pheM"
                     /locus_tag="OO564_002136"
     CDS             2587..2631
                     /gene="pheM"
                     /locus_tag="OO564_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6653260.1"
                     /translation="MNAAIFRFFFYFST"
     gene            2914..3897
                     /gene="pheS"
                     /locus_tag="OO564_002137"
     CDS             2914..3897
                     /gene="pheS"
                     /locus_tag="OO564_002137"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6653261.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            3912..6299
                     /gene="pheT"
                     /locus_tag="OO564_002138"
     CDS             3912..6299
                     /gene="pheT"
                     /locus_tag="OO564_002138"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6653262.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            6304..6603
                     /gene="ihfA"
                     /locus_tag="OO564_002139"
     CDS             6304..6603
                     /gene="ihfA"
                     /locus_tag="OO564_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6653263.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            6704..7684
                     /gene="btuC"
                     /locus_tag="OO564_002140"
     CDS             6704..7684
                     /gene="btuC"
                     /locus_tag="OO564_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6653264.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            7747..8298
                     /gene="btuE"
                     /locus_tag="OO564_002141"
     CDS             7747..8298
                     /gene="btuE"
                     /locus_tag="OO564_002141"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6653265.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            8298..9047
                     /gene="btuD"
                     /locus_tag="OO564_002142"
     CDS             8298..9047
                     /gene="btuD"
                     /locus_tag="OO564_002142"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6653266.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            9125..9589
                     /locus_tag="OO564_002143"
     CDS             9125..9589
                     /locus_tag="OO564_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6653267.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            9836..10538
                     /gene="ydiV"
                     /locus_tag="OO564_002144"
                     /pseudo
     CDS             9836..10538
                     /gene="ydiV"
                     /locus_tag="OO564_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            10602..12005
                     /locus_tag="OO564_002145"
     CDS             10602..12005
                     /locus_tag="OO564_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6653268.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(12042..12233)
                     /gene="hemP"
                     /locus_tag="OO564_002146"
     CDS             complement(12042..12233)
                     /gene="hemP"
                     /locus_tag="OO564_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6653269.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(12365..13411)
                     /gene="aroH"
                     /locus_tag="OO564_002147"
     CDS             complement(12365..13411)
                     /gene="aroH"
                     /locus_tag="OO564_002147"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6653270.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(13568..14401)
                     /gene="ppsR"
                     /locus_tag="OO564_002148"
     CDS             complement(13568..14401)
                     /gene="ppsR"
                     /locus_tag="OO564_002148"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6653271.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            14733..17111
                     /gene="ppsA"
                     /locus_tag="OO564_002149"
     CDS             14733..17111
                     /gene="ppsA"
                     /locus_tag="OO564_002149"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6653272.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(17168..18814)
                     /gene="fadK"
                     /locus_tag="OO564_002150"
                     /pseudo
     CDS             complement(17168..18814)
                     /gene="fadK"
                     /locus_tag="OO564_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(18871..19164)
                     /locus_tag="OO564_002151"
     CDS             complement(18871..19164)
                     /locus_tag="OO564_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6653273.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(19161..20450)
                     /locus_tag="OO564_002152"
     CDS             complement(19161..20450)
                     /locus_tag="OO564_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6653274.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(20506..21444)
                     /locus_tag="OO564_002153"
     CDS             complement(20506..21444)
                     /locus_tag="OO564_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6653275.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(21464..22228)
                     /locus_tag="OO564_002154"
     CDS             complement(21464..22228)
                     /locus_tag="OO564_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6653276.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5481 a         5505 c         5946 g         5471 t
ORIGIN      
        1 gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca tatgcaggaa
       61 gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg tgaactggaa
      121 gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg tccgttcatg
      181 atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga tgcaatgttc
      241 accacatctt ctgagaaccg tgaatactgc attaagccga tgaactgccc gggtcacgta
      301 caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt
      361 ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtgga
      421 tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga tgaagttaac
      481 ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa gatcgtcgtc
      541 aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg aaatgtggga tcgtgctgag
      601 gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca actgggtgaa
      661 ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga tcgtgcatgg
      721 cagtgcggta cagtacagct ggacttctcc ttgccgtctc gtctgagcgc ctcctatgtg
      781 ggcgaagaca acgagcgtaa ggtaccggta atgattcacc gcgcaattct ggggtcgatg
      841 gagcgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac ctggcttgcg
      901 ccagttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt taacgaattg
      961 acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa tgagaagatt
     1021 ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt ctgtggtgat
     1081 aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga cctgggaagt
     1141 atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg cagtcttaaa
     1201 caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt
     1261 atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt
     1321 ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc
     1381 gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc
     1441 tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag
     1501 gaaattaaat tccgtcctag tacagatgaa ggcgactatc aggtaaaact ccgcagcctg
     1561 attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag
     1621 atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa
     1681 ctggcagtag tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc
     1741 gctcctaaga agaaacagta aggccttcaa gtagcactat ctgtggagcc tttgggcttc
     1801 acaggcattg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt tattaaaatg
     1861 ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt
     1921 tttaagcaca agcacgctaa cctgcgtcac attctgacta aaaaagcgac caaacgtaaa
     1981 cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcttggt aatcgcgtgc
     2041 ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata gatacaggag
     2101 agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt
     2161 gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc
     2221 tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc gtcagttccg
     2281 tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt cttacagcaa
     2341 attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat
     2401 cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag cagctctggc
     2461 ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat
     2521 tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg
     2581 caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg aatccaggag
     2641 gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt
     2701 tttgtatgcg cgttttgaaa aataaaaagc cagtctatca ggcgtaattg tcgcagccag
     2761 tctggacacg gccagtgcgg agaaaccgga gcgtacttaa gtacgtgagg atttcgagca
     2821 ctgcccggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta agtccaacga
     2881 accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga actggttgcc
     2941 agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagataa tgtgcgcgtc
     3001 gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg tgagctgccg
     3061 ccagaagagc gtccggcagc tggtgcggtt atcaacgaag cgaaagagca ggttcagcag
     3121 gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg tctggcggcg
     3181 gaaacgattg atgtctctct gccaggtcgt cgcattgaaa acggcggtct gcatccggtt
     3241 acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac cgtggcaacc
     3301 gggccggaaa tcgaagacga ttatcataac ttcgatgctc tgaacattcc tggtcaccac
     3361 ccggcgcgcg ctgaccacga cactttctgg tttgacgcta cccgcctgct gcgtacccag
     3421 acctctggcg tacagatccg caccatgaaa gcccagcagc caccgattcg tatcatcgcg
     3481 cctggccgtg tttatcgtaa cgactacgac cagactcaca cgccgatgtt ccatcagatg
     3541 gaaggtctga ttgttgatac caacatcagc tttaccaacc tgaaaggcac gctgcacgac
     3601 ttcctgcgta acttctttga ggaagatttg cagattcgct tccgtccttc ctacttcccg
     3661 tttaccgagc cttctgcaga agttgacgtc atgggtaaaa acggtaaatg gctggaagtg
     3721 ctgggctgcg ggatggtgca tccgaacgtg ctgcgtaacg ttggcatcga cccggaagtt
     3781 tactctggtt tcgccttcgg gatggggatg gagcgtctga ctatgttgcg ttacggcgtc
     3841 accgacctgc gttcattctt cgaaaacgat ctgcgtttcc tcaaacagtt taaataaggc
     3901 aggaatagat tatgaaattc agtgaactgt ggttacgcga atgggtgaac ccggcgattg
     3961 atagcgatac gctggcgaat caaatcacta tggcgggcct ggaagttgac ggtgtagaac
     4021 cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt gcgcagcatc
     4081 cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg cggcgatcgc ctgctggaca
     4141 tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagct accattggtg
     4201 ctgttctgcc gggtgatttc aaaattaaag cggcgaaact gcgtggcgaa ccgtctgaag
     4261 ggatgctgtg ctccttttct gagctgggaa tttctgacga tcacagtggc attatcgaac
     4321 tgcctgcgga tgcgccgatt ggcaccgaca tccgcgaata cctgaaactc gatgataaca
     4381 cgatcgaaat cagcgtgaca ccaaaccgtg ccgactgctt aggtatcatt ggtgttgcgc
     4441 gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt ccggttggtg
     4501 cgaccatcga cgacacgttg ccgattacag tcgaagcgcc ggaagcctgc ccgcgctatc
     4561 ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg atgaaagaaa
     4621 aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc aactatgtac
     4681 tgctcgaact gggccagccg atgcacgctt tcgataaaga tcgcattgaa ggcggcattg
     4741 tggtgcggat agcaaaagag ggcgaaacgc tggtgctgct tgatggcact gaagcgaagc
     4801 tgaatgctga cactctggtc atcgccgacc acaacaaggc gctggcgatg ggcggcatct
     4861 ttggtggcga acactctggc gtgaatgacg aaacgcaaaa cgtcttgctg gaatgcgctt
     4921 tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat actgatgcgt
     4981 ctcaccgtta tgagcgtggc gttgatccgg tactgcagca caaagcgatg gaacgtgcga
     5041 cccgtctgct gatcgacatc tgcggtggtg aggctggccc ggtaattgat atcaccaacg
     5101 aagcaacgct gccgaagcgt gcaaccatca ctctacgtcg tagcaaactg gatcgcctga
     5161 taggccatca tattgctgat gagcaggtaa ctgacattct gcgtcgtctc ggctgcgaag
     5221 tgaccgaagg caaagacgaa tggcaggcag ttgcgccgag ctggcgtttc gatatggaga
     5281 ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac atcccggatg
     5341 agccggtaca ggcaagcctg attatgggta ctcaccgtga agctgacctg tcgctcaagc
     5401 gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt gatcacctac agcttcgttg
     5461 atccgaaagt gcagcagatg atccatccag gcgttgaagc cttactgctg gcaagcccga
     5521 tctctgttga aatgtcagca atgcgtcttt ctctgtggac cggcctgctg gcaaccgtgg
     5581 tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt ctgcgtttcg
     5641 taccagatac tcaggcaccg ttgggcattc gtcaggatct gatgttagcc ggtgtgattt
     5701 gcggtaaccg ttacgaagag cactggaacc tggcaaaaga gaccgttgat ttctatgatt
     5761 tgaaaggcga ccttgaatcc gttctcgacc tgaccggtaa actgaatgag gttgagttcc
     5821 gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc agcgatttat ctgaaaggtg
     5881 aacgtattgg ttttgttgga gttgttcatc ctgaactgga acgtaaacta gatcttaacg
     5941 gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg gtgcctcagg
     6001 cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg gtcgcagaaa
     6061 acgttcccgc agcggatatt ttatccgaat gtaagaaagt tggcgtaaat caggtagttg
     6121 gcgtaaactt atttgacgtg taccgcggta agggtgttgc ggaggggtat aagagcctcg
     6181 ccataagcct gatcctgcaa gataccagcc gtacactcga agaagaggag attgccgcta
     6241 ccgtcgccaa atgtgtagag gcattaaaag agcgattcca ggcatcattg agggattgaa
     6301 cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt ttgataagct tgggcttagc
     6361 aagcgggatg ccaaagaact ggttgaactg tttttcgaag agatccgtcg cgctctggaa
     6421 aacggcgaac aggtgaaact ctctggtttt ggtaacttcg atctgcgtga taagaatcaa
     6481 cgcccgggac gtaacccgaa aacgggcgag gatattccca ttacagcacg gcgcgtggtg
     6541 accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc caaagacgag
     6601 taatctgatc taactaaaaa ggccgctatg cggccttttt tcttttcact gtcgaagagt
     6661 caccgtaaaa tcaacgccat gacacttcag cagaacggat accatgctga cacttgcccg
     6721 ccaacaacag cgacaaaata ttcgctggtt attatgcctg tcagttttga tgctgctggc
     6781 gcttctctta agcctttgcg ccggtgaaca atggatttcg ccaggtgact ggtttactcc
     6841 tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt acgctggctg tattgctggt
     6901 tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa atcctctggc
     6961 agaacctgga ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg ccgcggtatt
     7021 gcttgggcaa gggcaactcc ccaactgggc gctagggctg tgtgcgattg ctggcgcgct
     7081 tatcatcact ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca gtcggttatt
     7141 gctggctggc gttgcattag ggattatctg cagcgcacta atgacgtggg ctatctactt
     7201 ttccacctct gttgatttac gtcagctgat gtactggatg atgggcggtt ttggcggcgt
     7261 agactggcgg caaagctggc tgatgctggc attgatcccc gtgttgttgt ggatctgttg
     7321 tcagtccagg ccgatgaata tgttagcact tggcgagatc tcggcgcggc aactgggttt
     7381 acccctgtgg ttctggcgca atgtgctggt ggcagcgacc ggctggatgg ttggcgtcag
     7441 tgtggcgctg gcgggtgcta tcggctttat tggtctggtg atcccacata ttcttcggtt
     7501 gtgtggttta accgatcatc gcgtattact tcccggctgc gcgctggcag gggcgagcgc
     7561 attgctgctg gccgatattg tagcgcgcct ggcattagct gccgcagagc tgcctattgg
     7621 cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa aagcaggacg
     7681 ttagccgcaa aaagacggtc tatgattaaa agctaagatt tttactgacc acacccagga
     7741 gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc
     7801 acgctggaga agtacgccgg taatgtgctg ttaattgtca atgtcgcctc aaagtgtggc
     7861 ttaacgccgc aatatgagca gttggagaat attcaaaaag cctgggccga tcgaggtttt
     7921 gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag cgatgaagag
     7981 attaaaactt actgtaccac cacatggggg gtgacgttcc cgatgttcag taagattgaa
     8041 gttaatggcg aaggacgcca tccgctgtat caaaaattga ttgccgcagc gccgaccgca
     8101 gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggccg tgcaccgctg
     8161 taccaggatg atattttatg gaattttgaa aaattcctag ttggcaggga cggaaaagtc
     8221 atccagcgtt tttccccgga tatgacgccg gaagatccca ttgtgatgga aagcattaaa
     8281 ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg gaatctaccc
     8341 gcctggggcc gctttcaggc gaggttcggg ctggggaaat cctgcacctg gtggggccga
     8401 atggcgcggg taagagtacc ttactggcgc gaatggccgg aatgaccagc ggtaagggaa
     8461 gcattcagtt cgcggggcaa ccactggaag catggtccgc aacaaaactc gcgctgcatc
     8521 gcgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg cactacctga
     8581 cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg
     8641 ctcttgatga caaactcggt cgtagcacca atcaactttc cggcggtgaa tggcaacgcg
     8701 tacgtcttgc tgcggtggtg ttgcaaatca caccacaagc caatcccgca ggccaattgc
     8761 tgcttcttga tgagccgatg aacagtcttg atgttgcgca acaaagtgcg ttagacaaaa
     8821 ttctgagcgc gctgtgtcag caaggactgg cgattgtgat gagcagtcac gatctcaacc
     8881 acacgttgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg
     8941 gacgcagaga agaggtgctc acgccgccaa atctggcgca ggcctatggg atgaattttc
     9001 gccgtctgga tatcgaaggt cacagaatgc tgatttcgac catctgattt cattagtatc
     9061 ccgaaaatcg ggtaattacg acgctaaatt aatgcaagaa taaaaaacag aggaaccgta
     9121 cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt gtagccacca
     9181 taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg ccggtttgaa
     9241 cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga cgcggcgcgg
     9301 tgtggactgt tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt tgcatctgcc
     9361 ccgtgaaacc aaacaacaag cctctattgg cacgcaaatt gataaagacg agttgctgcc
     9421 tggtgacctg gtctttttca aaacgggttc cggacaaaat ggtttgcatg taggtattta
     9481 cgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc gttcctcact
     9541 tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt gtgtcgaaaa
     9601 attgacggcg atgatacatc gccgtcaatt tactcctgaa tacagtagta ttttgcagac
     9661 aaaaattccg taaatggatt attcgtttat aatcaggata atctaaaaaa aggccccctg
     9721 aaaaagtggt taacatggaa caaatgaaag ttactccttc tgtaattgtt atgtcttatg
     9781 gcctcatccc ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa
     9841 gatttttttg gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca
     9901 gcaactcctt gtcggcgttg agttaattac ccatttctcc agtgaagatg gcacggttcg
     9961 tatcccgacg agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc
    10021 agagcagttg gaattactaa aatcatgcca acactttttt attcaacata aactctttgc
    10081 ctggcttaat ttaacgccac aagtggctac attattatta gacagggata attacgcagg
    10141 cgaactatta aaatatccgt ttattgagct gttgattaat gagaattacc cgcatctcaa
    10201 tgaaggaaaa gataatcgcg atcttttgtc cttatcgcaa atgtatcccc ttgtgttagg
    10261 caatttggga gcaggtaata gcacaatgaa agcggttttt gatggtcttt tcacccgcgt
    10321 aatgctggag aaaggaacaa atcacgcatc gctccttcga accgtttatt cgtgcgatcc
    10381 aggcgcaaat ttccccttgt tgcaattgca tcatcgcggg gggcattgat acgccagaaa
    10441 tattggcaca gattaccccc ttcgatttcc atgctctcca gggatgcctg tggcctgccg
    10501 ttcctataaa tcagataacg acgttggttc agcgataacc ctcctgtttg ctggtgttta
    10561 agacgagagt aaccgtctac actatcaaac agataggatc tatgaccctg tcttttatta
    10621 cccgctggcg cgatgaattg ccagaaacct atacagcact ttcccctacg cctttaaata
    10681 atgcccggct gatttggcat aataccgaac tcgctaacac gctgagtatt ccatcgtcgc
    10741 tgtttaaaaa tgccgcaggt gtctggggcg gcgaaaccct actgcctggt atgtcaccac
    10801 tggcacaggt ttacagtggt catcagttcg gcgtctgggc gggccaactg ggtgatgggc
    10861 gcggcatttt acttggcgaa caactgcttg ctgatggcac tacaatggac tggcatctga
    10921 aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg acgggcggtt ttacgttcga
    10981 cgatacgaga aagtctcgcc agtgaggcga tgcattatct gggcattccg acgacccgcg
    11041 cgttaagtat cgtcaccagc gattcgccag tgtatcggga aacggtggag ccaggcgcga
    11101 tgctgatgcg tgtggcacca agtcatctgc gctttggtca tttcgaacat ttttactatc
    11161 gccgcgagcc ggaaaaggtt cgtcagttgg ctgactttgc cattcgtcat tactggtcac
    11221 atcttgaaga cgatgaggac aaataccgtc tctggtttaa cgatgttgtc gcacgtaccg
    11281 cgtcgttaat tgcccaatgg cagacggtcg gctttgcaca tggggtgatg aataccgaca
    11341 acatgtcgct gctgggactg acgcttgatt acgggccgtt tggttttctt gatgattacg
    11401 aacccggttt tatttgtaat cactcggatc atcaagggcg ttatagcttt gataatcaac
    11461 ctgctgtcgc gttgtggaat ttacagcgtc tggcgcagac attgtcacca tttgttgccg
    11521 tagatgccct gaatgaggcc ctggacagct atcagcaggt tttgttgacg cattatggac
    11581 aacggatgcg gcagaaactg ggcttcatga cggagcaaaa agaggataac gcgctactga
    11641 atgaattatt cagtctgatg gcgcgagagc gcagcgatta tacccgcaca ttccgcatgc
    11701 tgagtctgac cgagcagcac agcgcggcgt caccgctacg tgatgagttt attgatcgtg
    11761 cggcatttga tgactggttt gcccgttatc gggggcgttt gcaacaagac gaggttagcg
    11821 atagtgagcg tcaacaactg atgcaaagcg ttaacccagc tctggtattg cgcaactggt
    11881 tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat gatggaattg caccgcctgc
    11941 atgaggcgtt gcgaaatcct ttcagcgaca gagatgatga ctatgtcagt cgtccacctg
    12001 actgaggtaa acggctggaa gtcagctgct cgagttaaag gctacttagt taacagcagc
    12061 ttgccagcct gagttttacg gagcagatat tcttgcccgt catgatcgat aatcagtttg
    12121 ccatccggac ctaacagtgt ctggctggta attcgccgga tgggctgcgg ggaagcggtc
    12181 ttgtgattgg catccgtttc aggcgtgagt tttctgctat ccgtataacg cataaaaatt
    12241 aataataatt gatataacaa tgattcctat tatcaatatt atggcaggcc agagcaagca
    12301 gtaatgtgtt gagaagacaa caagttgcgc agaaatgccc atcccatcag gaatgggcac
    12361 gcattcagaa gcgggtatct accgcaaagg cgagtttttc gaccaggcgt tcggtatctt
    12421 cccagcccag acacgggtcg gtaatggatt gaccgtaagt gagcggctga ccgccgacga
    12481 ttttttgcgt tccttcgcgc aggaaacttt ccgccataat tccagcaatc tccgtagagc
    12541 cattgcggat ttgctgacaa atatcctcac aaacttctaa ctggcgacgg tgctgcttct
    12601 ggcagttacc gtggctgaaa tccaccacca gatgttcagg taaatcaaac tcgtgcagcg
    12661 tatcgcaggc tgcggcgata tcatcggcat gataattcgg ttttttgccg ccacgcataa
    12721 taatgtggcc atacgggttg ccgctggtct gatagatggt catctgacca tttttgtctg
    12781 gcgagaggaa catatggctg gcgcgggctg cgcggatagc atccacggca atccgcgtgt
    12841 tgccatcggt accattttta aaacctaccg gacaggagag tgctgaagcc atttcgcggt
    12901 ggatctgact ttcggtagta cgagcgccaa tcgcgcccca actgattaaa tcagcaataa
    12961 actgaccggt caccatatcg aggaactcag tcgcggtagg gacacccagc tcatttacct
    13021 gtaaaagtaa tttgcgcgcc agctccagac cgtgatttac ccgatagctg ccgtttaaat
    13081 ccggatcgga gattagccct ttccagccga caacagttcg tggtttttca aaataggtgc
    13141 gcattacgat ttccagccgt gactggtact ggttgcgcag cgactgcaga cgggtggcgt
    13201 actccattgc agcggtgaga tcgtggatcg agcaggggcc aataatgacc aacagtcgct
    13261 tatcttcacc attcagtatt ttttcaattc tgcggcggga gtcggtgaca tgggtggcga
    13321 cgccaggcgt tacgggatac cgtagcgcga gttcggcggg cgttaccagg ctctcaatac
    13381 gcgcagtacg gagttcgtca gttctgttca ttacaagtct cagaaatttg ttgcttctac
    13441 ggcaggacta ccggaaagtg atgcgcatca cgataaacca atcgcagctg atttcaagtg
    13501 cgaggtgtgt cacacctcgc cgggtggtta gcatgataac aaaaaaacaa accaatacac
    13561 tagttctcta gtacattcgg cgactaaggc ccatgatatc gaggatcttg gtggcaatct
    13621 cttctaccga ataattggta ctgttaatcc acgggatctg atttttacgg tacaaggctt
    13681 ccacttccgc gacttccatc ctgcactgac gaagcgaggc atagcgactg ttctcccgac
    13741 gttcctcgcg aatcgccgcc agacgttccg ggtcgatagt caggccgaac aatttatgct
    13801 gaagcggttt gagcgacgcg ggtagcacca gattatccat atcgtcggca ataaaggggt
    13861 agtttgccgc gcggatacca aattgcattg ccagatacag actggtgggg gttttaccac
    13921 agcgagaaac accgagcagg atcacctgag cctgatccag attgcgcaac gaaatgccgt
    13981 catcgtgggc gagggtgtaa tcaatcgccg caatgcgcgc atcatattta ttgagattat
    14041 tagggttaag gccatgggta cgatgagcaa tcggcgttgg atccagtttc atctcttgtt
    14101 gtagcggggc aaccagcgcc tgaacgatat cctggcaaaa gccttcactt tgcaagatga
    14161 tggcgcgaat ctccggcaac acgatggagt agaagaccag cgggcgcacg cctgtctggt
    14221 gataaattgc gtcaatctga tccttcactg cccgtgcacg gctctcattt tcgacaaacg
    14281 gcagcgtgat gctgctgata gtgacgggaa attgtgacat tactgcgtgt cctaatacct
    14341 ccgcagttat tgccgtacca tcagaaatat aaaaaacgtg gcgatcaaca gcattatcca
    14401 ttttgttctt cccgtgaagc agacataatt ctatgaaagc ataaattaaa agcgcacaga
    14461 agcgtaggac gtaatgtctg gtttataaaa tgaaccttca ttttattttt tatgaaaaca
    14521 gcatttcatt tttatggttt cgtttatacc gatggtttat gtggaaattg tcgaatagag
    14581 caggtttgcg caacgctggg atcagtctta aaaagtaaaa aaatatattt gcttgaacga
    14641 ttcaccgttt ttttcatccg gtaaaatatg caaagataaa tgcgcagaaa tgtgtttctc
    14701 aaaccgttca tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg
    14761 tgctttggta taaccaactc ggcatgaatg atgtagacag ggttgggggc aaaaatgcct
    14821 ccctgggtga aatgattact aatctttccg gaatgggtgt ttccgttccg aatggtttcg
    14881 ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt
    14941 atgaactgct ggataaaacg gatattgacg atgttactca gcttgcgaaa gcgggcgcgc
    15001 aaatccgcca gtggattatc gacactccct tccagcctga gctggaaaac gccatacgcg
    15061 aagcctatgc acagctttcc gccgatgacg aaaacgcctc ttttgcggta cgctcctccg
    15121 ccaccgcaga agacatgccg gacgcttcct ttgccggtca gcaggaaacc ttcctcaacg
    15181 ttcagggttt tgacgccgtt ctcgtggcag tgaaacatgt atttgcttct ctgtttaacg
    15241 atcgcgccat ctcttatcgt gtgcaccagg gttacgatca ccgtggcgtg gcgctctccg
    15301 ccggtgttca gcggatggtg cgctccgacc tcgcatcttc tggcgtgatg ttctccattg
    15361 ataccgaatc cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggggaaa
    15421 tggtcgtgca gggtgcggtt aacccggatg agttttacgt gcataaaccg acactggcgg
    15481 cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc atggtttacg
    15541 cgccgaccca ggagcacggc aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg
    15601 acatcttctc gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg
    15661 agaaacacta cggtcgccca atggatattg agtgggcgaa agatggccac accggcaaac
    15721 tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt
    15781 atacgctgca ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg
    15841 gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc gaacctggcg
    15901 acgtgctggt tactgacatg accgacccgg actgggaacc gatcatgaag aaagcatctg
    15961 ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactgg
    16021 gcattccggc ggtagtgggc tgtggagatg caacagaacg gatgaaagac ggtgagaacg
    16081 tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta
    16141 gcgtgaaaag ctccagcgta gaaacgatgc cggatctgcc gttgaaagtg atgatgaacg
    16201 tcggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc gagcgaaggc gtgggccttg
    16261 cgcgtctgga atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt
    16321 ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt
    16381 ctccgcgtga attttacgtt ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt
    16441 tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaacc
    16501 tggtcggtgg tgagcgttac gagccagatg aagagaaccc gatgctcggc ttccgtggcg
    16561 cgggccgcta tgtttccgac agcttccgcg actgcttcgc actggagtgt gaagcagtga
    16621 aacgtgtgcg caacgacatg gggctgacca acgttgagat catgatcccg ttcgtgcgta
    16681 ccgtagatca ggcgaaagcg gtggttgaag aactggcgcg tcaggggctg aaacgtggcg
    16741 agaacgggct gaaaatcatc atgatgtgtg aaatcccgtc caacgccttg ctggccgagc
    16801 agttcctcga atatttcgac ggcttctcaa ttggctcaaa cgatatgacg cagctggcgc
    16861 tcggtctgga ccgtgactcc ggcgtggtgt ctgaattgtt cgatgagcgc aacgatgcgg
    16921 tgaaagcact gctgtcgatg gctatccgtg ccgcgaagaa acagggcaaa tatgtcggga
    16981 tttgcggtca gggtccgtcc gaccacgaag actttgccgc atggttgatg gaagagggga
    17041 tcgatagcct gtctctgaac ccggacaccg tggtgcaaac ctggttaagc ctggctgaac
    17101 tgaagaaata aaataaatcc ccggcggcgt tcagtcgccg gggttatgtg atccccgaag
    17161 atgaaactta ttcaatctct tcacagacat cctgcgttaa tcgccgtata atatctttcc
    17221 ttaacaaaaa cttttgtatt ttatctgagg cagtgcgcgg tagtttttcg attaccacga
    17281 tatgttcagg atatttatat tttgcgaccc gtttacggct aaaaaaagcc accatctctt
    17341 ccagcgataa tgaatgatgc ggcgctttct gcacgacata agcgcatgat cgttcaccta
    17401 aacgttcatc gggcattgca accacacagg catcgtgaat tttaggatgc tgcaataaaa
    17461 tatcttccac ttcacggctg ctaatatttt cgccgccgcg gacaataata tcttttttgc
    17521 gtccggtaat ttttatatag ccagcctcat ccatacggca gagatcgccg ctgtaatacc
    17581 agccttcttc atccagcgca cgggcggtta attcaggttc atcaaaatac cccataaaca
    17641 cattggggcc acgcgatgtt tcttcacctt cgcaacctgg cggtaaggtc ttgcgtgcgt
    17701 catcgaccac tttaatctct acacctgcgg cagcgtaacc atcggtatgc ataaagcgcg
    17761 acaaagaatt atcgagattc accaccgcat gcggcgaact ttctgtggaa ccataaacac
    17821 ttaataattt aatgctgcgc tgctggcatt cgcgcgccac ttttttgggg attgtggtac
    17881 cgccgcaaag aaagaagcgc agcgctgaaa ggtctgcggg ttgtttctct agtaaattca
    17941 aaagatcata gacaaacggc gttgcgccta gcatacaggt gcaacgctgc tgctcaagca
    18001 gcgcgagaca cgcatcagga gtgaaaatat ctaacaacac gctgcgagcg ccaattaaga
    18061 atggtgccgt tacgccatgc agaaagcccg ttgcgtgacc aagtggcgca ggcatcataa
    18121 agacatccag ccaggtcaga ttcagccgcg cgcaataagc ccgctcactg gcgagaatat
    18181 tgttatgcgt tagcatcacg ccctttggca gaccctcggt tccggaggta aacagcaccg
    18241 cagctaattc atcgccgtgg gtcgttatcg ccgtggtcag tgggatattg tcggcgataa
    18301 tctgacttaa tgagagggaa gaggtggcgg gagccagttt gtccacgccg acaatttgtt
    18361 gtagttgtgg aagctgattt tgcagcggca ggattaaatc taccggacgc gtttgtttaa
    18421 acaacgtcgg tgcaaagaac atttttgcct gacacttatt gagcacccac accagttctg
    18481 cttcccgcca ggaaggcaac agcggcacgg aaaccgcacc gattttcagg caggcaagat
    18541 agataacggt aaattcacac cagccaggca gttgaaatgc gatgcgatcg cctgattcaa
    18601 tacccttcgc taacatccag tttgccagac agctcgcggc gtgatcgagc gcgctatagg
    18661 tgtacgatgc accatgatta tcgaccacgg caattttgtc tggcatcgca cgagcggtct
    18721 gctgtcagta atcggccagt gaagcatcgc cccatagccc ttgctgacga tacgccgcac
    18781 gacgttgttc gttaaacgtt aatgtcactt tcattttgga ctctcgaaac agaacatcag
    18841 gcccgagttg cgggcctgtg ggatgcatga ttagccgtag cgaaaatcaa taccgaaggt
    18901 gcctgcggga tattgccact gttcgagaat agtgttaccg cacagcaccc gacaggtgcc
    18961 gcactccaga caaccggcgg gatcaaaatg aatgtttcct acgtcatcct gcttataaag
    19021 tccggcaggg caggctttca ttaatttacg gaattcattg atatcgggat tttccgtcag
    19081 aatgatatgc gggtggccct catcaacatg gaatttattg acgcctaatt tgatgtcgac
    19141 gttaaccgta gcgttctggc tcatagcgcg gttgctccct taatgccatc tttcagcaag
    19201 ttgatcagcc caattttctt cgcgtgtccc atgatcattt tgcgtaccgg ctggtttggt
    19261 ttgccgtcaa tggtgaacat ctcgttcatg atgtcggcta ccattcgtgg gtattggcta
    19321 aacaggcgcg ggttttccat cagcgccggg atcttgcgaa aatgctgcat atcacgcatg
    19381 acgcagcttt gttccagctc acgtttgtat tgcgccagac tgttggcgga gaaatccgcg
    19441 cgttctttgg cggcgatcac cgttgtggcg gcagcctgag ccgatgcaat ggctaaatcc
    19501 atgccgcgga ccgtaaaacc caaattcagg cagaagcctg cggcgtcacc aacgatcatc
    19561 acgccctcgt taaccagttg cggcaccatt gccagaccgc cttccggcac catatgcgcg
    19621 gaatattcaa gcagtttgcc gccgctaatc agcgggcgaa tggcggggtg ttgtttaaaa
    19681 tcttccagca tttgcggcac gcttttttgc gcatgggcga tatcacccaa tccacaaacc
    19741 agccccaagg atatggaatc cttgttggta tagataaatc ccccgcccat caggccgtca
    19801 gaaggggaac cggcaaacag ccaggcggca ccttcctcgc ccgtaatatt aaagcgatcg
    19861 ttgatctgtt ctggtgtgag gccaataacc tctttaacac caacagcgta atgatgcgga
    19921 tcggaagcgg gaaccattcc cagcgagcgg ccaagcattg agttaacgcc atcagccaga
    19981 atcaccacat tcgcttcgag aatatcatcc ccagcctgca cgccagtgac cttgtttcct
    20041 tcacgaacca gcgcgtcgac gcgaacgcct gggataaact gcgcgcccgc ctgctcggct
    20101 tgttccatca accacgggtc cagacgatta cgcaatacgg tataagatgc gtgttgcgga
    20161 acatctggtt gctcgcggtg aaaatcgagg gtaacggcgc tttcttcggt taagaaggag
    20221 attttctcgc gtgtgacctt gcgttctacc ggcgctgata ctgcaaagcc tggaatgatt
    20281 gcttcaagtg tgtgggcata aagacgcccg ccggtcatgt ttttacatcc ggcactgtcg
    20341 ccgcgttcta tcaccaggac atccagcccg gctcgtgcca tgaccagtgc ggcaacgcta
    20401 ccagcaacgc ccgcaccgac cacaatggca tcaaatttgt catccgacat gcgttatgct
    20461 cccttcccgc tgcgtttaca gcgggagatg tggttgaaag gggaattagc ggctcaactg
    20521 gctaatcagg gcagggacga ctttgtagat atcgcccacc agaccgtagt cggcatagtt
    20581 gaagattggc gcatttttat ctttattgat ggcgacaatc acttttacgc cgttgccgcc
    20641 aaccatatgt tggatctgcc cggagatccc ctgcgtcagg tagagatcgg atttcagcaa
    20701 cacgccggag acaccgatat aacgttcacg ctccatccag ttctcgcctt cggcaattgg
    20761 acgtgaacag ccgacttcag cattcagcac cgccgccagt tcgtggacca ttttcaggtc
    20821 atcctgcgcc gccagaccac gaccgacgcc aaccacacgt tttgctttgc tcaggtccac
    20881 gctgcttgcg gctttcgcac ggcgttcgcg acagagaatt tcatgacgcg gagcaacata
    20941 aggtaccgtt tctgtcgggc actgatgaga ggtatcactg gtgcacggtt cctgaacacc
    21001 gggtgcaagg gaaatgatcg ccagcgggct gttgatcttt tcctgagcga acgctaaccc
    21061 gccatacatc cggtgttcgg cgcaaatgtg accatcgaca atatccaccg ccgtggcatc
    21121 gttcaccagc gccgcattca gttgcacact taaccgtgct gccagagctt taccacgttt
    21181 cgtcgcggcc aacagcaaca tagcggggtg tttatctttc agcagggcag caatgctttc
    21241 ggcgtaattt tcagtgcgtt gcagcgcgtc gttttgctca agaacataaa tacattttgg
    21301 accataaggc ataactgcct gcgcctggtc ggtattttgt acaatggcat acacctgttg
    21361 gccccattgc tgagcgccgc caaacagttc agcataacgt tcaggattat cgctaaagac
    21421 ccagacgctg tttaattgac tcatcgtttc ttctccgtag gcttcagttc agggctttct
    21481 tcagatgttc cgccagctcg gcaatggcct ccggcgaatc gttatcgaga atgatgtgct
    21541 tacgttctgt ttgcggcggt acgcgaatgc cgaccagttc agcaagtggc gcgctctggc
    21601 tccagtcaat atcacttgcc tgccactgat ttaccggttt tttacccgcg ccgagaatgg
    21661 ctttcatcga aggaatgcgt ggcacgttaa tatcggaggt gacgcagagc acggctggaa
    21721 cagagagttc aataacttca acatcatctt caagcgtgcg ttcaatcacc agtgtattgc
    21781 cctgacgctg aatagcactc actgcattaa tcaccggaag ttgcagaatt tctccgacca
    21841 gcaagccaac ctgctgggca taaaggtcgc cggaaccttc accaaagatc agtaaatcga
    21901 agccgatctt ttcaattgct gccgccagcg cttttgcggt atcgagaggc agtgcatgtt
    21961 caagttgcgc atcctgcacc aaatacaggc tgtgcggccc gcgggatagc acgtctttgc
    22021 gcactttcga gttctgcaac aatgagccgc caacggtcag cgcggctatc tcatcgtcat
    22081 ctgttgcgag ctggcttgca gcttcaatgg cattgagatc gaactggctg attttggcgt
    22141 cggcattgtc gaaattcagg gtgtattctg gagtgacaac aatgtcctgt tcttcaggca
    22201 ccagcttaaa gcaggttatt attttcatga tttctcctgt gagtcgcata cggaaccatc
    22261 tccgatggaa ggtaaaaatg gcctgaaaat agtgtgcgat agcggtgcgg ggggttcgac
    22321 ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa atgcgattgc taaaacaaaa
    22381 gtgcaatttg cggcaatata gtt
//
LOCUS       DAJTYI010000058        22086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYI010000058 DAJTYI010000000
VERSION     DAJTYI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1902)
                     /locus_tag="OO564_002155"
     CDS             complement(<1..1902)
                     /locus_tag="OO564_002155"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6653277.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMV"
     gene            2034..3143
                     /gene="apbC"
                     /locus_tag="OO564_002156"
     CDS             2034..3143
                     /gene="apbC"
                     /locus_tag="OO564_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6653278.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3405..3686
                     /locus_tag="OO564_002157"
     CDS             3405..3686
                     /locus_tag="OO564_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6653279.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            3979..4521
                     /locus_tag="OO564_002158"
     CDS             3979..4521
                     /locus_tag="OO564_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6653280.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            4602..5276
                     /locus_tag="OO564_002159"
     CDS             4602..5276
                     /locus_tag="OO564_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6653281.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5292..7772
                     /locus_tag="OO564_002160"
     CDS             5292..7772
                     /locus_tag="OO564_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6653282.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            7788..8821
                     /locus_tag="OO564_002161"
                     /pseudo
     CDS             7788..8821
                     /locus_tag="OO564_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(8903..9241)
                     /gene="rcnB"
                     /locus_tag="OO564_002162"
     CDS             complement(8903..9241)
                     /gene="rcnB"
                     /locus_tag="OO564_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6653283.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9460..10302)
                     /gene="rcnA"
                     /locus_tag="OO564_002163"
     CDS             complement(9460..10302)
                     /gene="rcnA"
                     /locus_tag="OO564_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6653284.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10423..10695
                     /gene="rcnR"
                     /locus_tag="OO564_002164"
     CDS             10423..10695
                     /gene="rcnR"
                     /locus_tag="OO564_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6653285.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      10787..10883
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10918..11706
                     /gene="thiM"
                     /locus_tag="OO564_002165"
     CDS             10918..11706
                     /gene="thiM"
                     /locus_tag="OO564_002165"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6653286.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            11703..12503
                     /gene="thiD"
                     /locus_tag="OO564_002166"
     CDS             11703..12503
                     /gene="thiD"
                     /locus_tag="OO564_002166"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6653287.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12568..13386
                     /locus_tag="OO564_002167"
     CDS             12568..13386
                     /locus_tag="OO564_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6653288.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13438..14184
                     /locus_tag="OO564_002168"
     CDS             13438..14184
                     /locus_tag="OO564_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6653289.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14158..15123)
                     /locus_tag="OO564_002169"
     CDS             complement(14158..15123)
                     /locus_tag="OO564_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6653290.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15120..16123)
                     /locus_tag="OO564_002170"
                     /pseudo
     CDS             complement(15120..16123)
                     /locus_tag="OO564_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16120..16596)
                     /locus_tag="OO564_002171"
                     /pseudo
     CDS             complement(16120..16596)
                     /locus_tag="OO564_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16750..17802
                     /gene="fbaB"
                     /locus_tag="OO564_002172"
     CDS             16750..17802
                     /gene="fbaB"
                     /locus_tag="OO564_002172"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6653291.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18110..18964
                     /gene="gatY"
                     /locus_tag="OO564_002173"
     CDS             18110..18964
                     /gene="gatY"
                     /locus_tag="OO564_002173"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6653292.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            18993..20260
                     /gene="gatZ"
                     /locus_tag="OO564_002174"
                     /pseudo
     CDS             18993..20260
                     /gene="gatZ"
                     /locus_tag="OO564_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20270..20722
                     /gene="gatA"
                     /locus_tag="OO564_002175"
     CDS             20270..20722
                     /gene="gatA"
                     /locus_tag="OO564_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6653293.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            20753..21037
                     /gene="gatB"
                     /locus_tag="OO564_002176"
     CDS             20753..21037
                     /gene="gatB"
                     /locus_tag="OO564_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6653294.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21041..22086
                     /locus_tag="OO564_002177"
                     /pseudo
     CDS             21041..22086
                     /locus_tag="OO564_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5742 a         5111 c         5600 g         5633 t
ORIGIN      
        1 caccataatg gtgtgacgac caatcagtgc ctgcggatcc gggtaagcag aacgaatacc
       61 ggagaagaca ttgcgttttt caccgccgag atccagcgtc aggcgcagca gtttgtcaga
      121 accttcaaca aactctgcgt tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc
      181 gtcaaaggtg atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggt
      241 agtggctttc acttcttctt tagaggcttc caccagagct tcaacctgtt tcatatcgat
      301 gcggttatac agcgccttga acggattcac tttgtggccc agcagcggtt gctggatacc
      361 atcccaggtc agttccgtat tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac
      421 cggcttcagg taagtcatca gcacgcggaa caggttgatg cccattgagc aaatcgcctg
      481 caggtcagca tcgcgacctt cctgttttgc gactacccac ggagcctgct cgtcgacata
      541 gcggttagcc aggtcagcca gtgccatgat ttcgcgcact gctttaccaa attcacggct
      601 ttcccacgct tcaccaatca cttcagcggc atcggtaaag gttttgtaca gctcagggtc
      661 agccagctcg cttgccagca caccgtcaaa acgcttgttg ataaagcccg cattacggga
      721 ggccaggtta accactttgt tcacgatatc ggcattcaca cgctgaacga aatcttccag
      781 gttgagatcg atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct
      841 gtcagcgtca aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga
      901 catcttcgcg ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa
      961 gttgctgcct tccagcatgg caggccagaa caggctgtgg aagtaaacga tatctttacc
     1021 gatgaagtgg tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct
     1081 gtcgccgcgc ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag
     1141 ccagacgtag aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc
     1201 gcgggagata tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tgtttgccac
     1261 ctgctcctgc aacgcaccgc tgcgggtcca tgcctgcagc atttcgctga aagagggcag
     1321 atcaaagaag aagtgttcag aatcacgcat cactggtgta gcaccagaaa ccaccgattt
     1381 cggctcgatc aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta
     1441 ttgatccggg gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat
     1501 gcctttttcc ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttttcttt
     1561 caggcgagag tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata
     1621 gttgtcatag ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc
     1681 aatcatctgc tccggggtga taccgagctg ctgagctttc agcatgatcg gtgtaccgtg
     1741 ggcatcgtcg gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca
     1801 gacatcagcc tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg
     1861 cagtgcgcac gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta
     1921 atagtgaaaa ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata
     1981 ttcttcaggt aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg
     2041 aacaatccca ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca
     2101 attttcagca cccaaccctg aagcataacc tcaccacgct gaaagcgttg caccatgtcg
     2161 cctggatgga cgacacgttg catgtggaac tggttatgcc gtttgtctgg catagcgcgt
     2221 tcgaagagtt aaaagagcaa tgtagcgccg aactgctgcg catcaccggg gcgaaagcta
     2281 tcgactggaa actgtcgcat aacatagcca ctctgaaacg cgtgaagaat cagccgggaa
     2341 ttaacggtgt gaagaacatt atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt
     2401 ccacggcggt aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg
     2461 acgccgatat ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa
     2521 cttcgccgga cggtacccac atggcaccga ttatgtctca tggcctggca accaattcaa
     2581 ttggttatct ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg
     2641 cgttaatgca gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata
     2701 tgccgccagg caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg
     2761 cagtcgtggt caccaccccg caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga
     2821 tgttcgagaa agtcgaggtt ccggtgctcg gtattgtcga gaacatgagt gtgcatattt
     2881 gcagtaactg tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg
     2941 aaaaatacca tacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc
     3001 tcgataaagg cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc
     3061 gccagctggc tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attcctggcg
     3121 agatttcctt ccgcgcggtc taacgcctat taaccattct ggttattctt catacatcgc
     3181 agagatgtta tattgatgcg gtgtatttgt ttttgccgca ttaatgaaca cttatttaat
     3241 actgcgaata ttctgcggac cttcgtccga aactctacaa agtcgagtga tcgtacaggc
     3301 aatgcttcat tatcatcctt cgtgctgtgt agtggatgta cagctatagt ttgttatgca
     3361 ctcattctat ccaggcataa cctttactac agggataacg ttcaatgaat aagtattggc
     3421 tgtcaggaat tatttttctt gcctatggat tggcttctcc cgcagtctct tcagagactg
     3481 ccacattaac gattaatggc aggatcagtc ccccaacatg cagtatggct atggttaata
     3541 gtcaaccaca acagcattgt ggtcagttaa cgtataatgt tgacacccgt catcaggttt
     3601 cttcgcccgt caaaggtgtt acgacagaag tggttgttgc aggtagcgat agcaaacgtc
     3661 gaatcgtgtt aaatcgctac gattaatttg gcaacctgat tttaattgca tttagaaaga
     3721 cgaaatcatt tatataaatg atttcgtctt ttttattgat atcaacaata ccatttacat
     3781 aaatctacat taattaatag atgtgataaa tatttaatat tcttaagtat ttgttgtatt
     3841 tatattgcat tggttaattt gttttaaatc aataaaacaa tatgtttaca ttgcggcagc
     3901 ttttttgatg tgagaaattt gtttccaaga gatacagact cttaacaacc aaaaataaat
     3961 tttattaagg aacatattat gaaacgttca attattgctg ccgctgtctt ttcttctttt
     4021 tttatgagcg ctggagtatt tgctgcagac gttgataccg gaacattaac cattaaagga
     4081 aatattgcag aatctccgtg taaattcgaa gcgggtggtg attcagtaag tattaatatg
     4141 ccgactgtac caaccactgt ctttgaaggt aaagctaaat attctaccta tgatgatgca
     4201 gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa
     4261 ctctcgttga tcacaaacga caaaataacc ggtaacgata aggcgatagc cagtagcaac
     4321 gataccgtgg gttactatct ctatttaggt gataacagcg atgtcctaga tgtttctgca
     4381 ccttttaaca ttgagagtta taaaacagca gaaggtcaat atgctattcc gtttaaagca
     4441 aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc ttctctggtt
     4501 atgcgtgtag cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa taaacatggc
     4561 cgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga ttattatctt
     4621 tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac gggacgcgca
     4681 ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt
     4741 cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca gaaaaaattc
     4801 aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattca gggcagcaag
     4861 taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt ttttatctga
     4921 atgttctgga tattccacca aatagtccag agcaagaagg caagaatgca ctgaagtttg
     4981 cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg gtaaataaag
     5041 cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata aaaaatgact
     5101 cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc aattatgaaa
     5161 ctattatgat tgccccctta gaaagtcaga gtgttaatat caaaagtaat aatgcaaata
     5221 actggtatct gaccattatc aatgaccacg gcaactatat tagtgacaaa atttaattgc
     5281 aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg
     5341 gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga
     5401 aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg
     5461 acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca
     5521 acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt aatagcgata
     5581 acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata
     5641 gctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac
     5701 tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg ttttatactt
     5761 cttattatgt gagtcagtat tacagcgact ataaagcgtc gggtaacaac aagagtacat
     5821 atgtacgttt taacagcggg ttaaatttac tggagtggca actgcattct gatgccagtt
     5881 tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg
     5941 gatttgccca atttctcggc acgcttcgcg tgggtgatat gtacacatca agcgatattt
     6001 ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact
     6061 cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg ttggtaacta
     6121 ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg tttgcgatta
     6181 cagatttgca gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg
     6241 gctcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg
     6301 tgtcgaaata tgattttgcg gcgggccgta gccatattga aggggcgagc aagcaaagtg
     6361 attttgtcca ggcaggttat cagtatggtt ttaataactt attgacgctg tatggcggca
     6421 cgatggttgc taataattac tatgcgttta ccctcggaac gggttggaac acgcgcattg
     6481 gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg
     6541 acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg
     6601 gtctggcggc ctggcgttat tcgtcgcgtg attaccggac atttaacgat cacgtttggg
     6661 caaacaataa agataattat cgccgtgatg aaaacgatat ctatgacatt gccgattatt
     6721 accagaacga ttttggccgt aaaaatagct tttctgccaa tatgagccag tcattgccag
     6781 aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca
     6841 gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc tataccctcg
     6901 cggcaagcca ggcgtatggc gagaatcatc atgaagagaa acgttttaat atttttatat
     6961 caattccctg tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta
     7021 actcaacgac gtttgatgat cagggttttg cctcaaataa tacgggatta tcaggaacag
     7081 tagggagtcg ggatcagttc aattatggtg tcaacctgag tcatcaacat cagggaaatg
     7141 aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt
     7201 atagtcagtc gagtacttat cgacagactg gagccagtgt ttcagggggc attgtcgcct
     7261 ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc
     7321 caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag
     7381 tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc
     7441 aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcccct tatcgcggcg
     7501 cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa
     7561 gagcggatgg tcaaccatta acgtttggtt atgaagtcaa tgatatccat ggtcataata
     7621 ttggcgttgt cggccaggga agccagttat ttattcgcac caatgaaata ccgccatcgg
     7681 ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga
     7741 ttgatgaaag tagaaattat atttgccagt aatagtcagg tggttctatg gaaattcgca
     7801 taatgctatt tatattaatg atgatggtta tgcctgtgag ctatgcggca tgttatagtg
     7861 agttatctgt tcagcacaat ttggttgttc aggggaattt tgcacttact caaacacaaa
     7921 tagcgacata tgagcataat tttaatgatt cgtcatgcgt gagtacaaat actatcaccc
     7981 ctatgagccc atcggatatt attgttggac tttataacga taccattaaa ttaaatttac
     8041 attttgaatg gaccaataaa aacaacatca cgttgtcaaa taatcagagc agtttcacca
     8101 gtggttattc ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg
     8161 ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt atccagtgct tcatcatcga
     8221 cacgcgggag tgccgcagta caatttctac tgtgtttatt aggtggcaag tcatgggatg
     8281 catgtgtaaa tagctacaga aatgcattgg cacaaaatgc aggtgtctat tccttcaatc
     8341 tgacattgtc atacaacccg ataaccacaa cctgcaaaca ggacgattta ttaattactt
     8401 tagacagtat tcccgtttca caattaccag ccacaggtaa caaagcaaca ataaatagta
     8461 aaaaaaggga tattattctg cgttgtaaaa atttattagg tcaacaaaat caaacatcac
     8521 ggaaaatgca ggtgtattta tcaagttctg acttgttaac caacagcgac acaatactga
     8581 aaggtgcgga agataatgcg taggatttat tcttgaaagt aatggttcgc cagtcacact
     8641 tttaaatatc actaacagca gtaaaggata cacaaattta aaagaaattg cggcaaagtc
     8701 aaaacttaca gatacaacgg tttcaattcc gataacagcc agttactacg tctacgatac
     8761 aaacaaagtt aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta
     8821 ataacaagca agacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc
     8881 cctcatgatg agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa
     8941 tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg
     9001 tcccggcatc aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg
     9061 tgcggtacat ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac
     9121 tacttaactc atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg
     9181 cccaggcggc actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca
     9241 taaactctcc actttgggga tctgtaatat cagtcgcata tctttcctgc catacaatat
     9301 aactgacaag cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg
     9361 atgccctaaa agcatcctta gcgagcagag agtcagcgga aatagctgat ttaagaatca
     9421 tcctgggctg aagataacat gcaagcctaa aggattgagt tatcgcatta tgcccatgaa
     9481 gccatgtacg cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc
     9541 gcgtttagcg agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc
     9601 tgcgccaacg cctacggtga caagcgttaa cgccaggcca atgctgaaac tgacgaccag
     9661 tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc aacagcacgg taattgctgc
     9721 cgggcagggg ataaggccac cggttaagcc aaataacaaa atttgccagt tgatgacctc
     9781 tctaccatca aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg catcctgata
     9841 ctcgccatgt tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt ggtcttgatg
     9901 atgttcgtga tgatggtgat gatgctcata atcatgcccg tgcatattct ccagccagtt
     9961 gcgttcgccg cgccaggtac gccagaacat ccagaacacg gtgccaataa tgatcactgc
    10021 ggaaatcagc tggagccacg gttctgctga ttgagcagta aagcgcttac tgatcaccat
    10081 cccgccaaag gcaatcaacc agaccaccgc ggtatgcgaa atggttgctg ccagtccgag
    10141 catcaccgcc tgtttaatgg tgcctttgat ggcgatgata aacgccgcca tcatcgtttt
    10201 tgagtgccct ggttccaggc catgaagcgc accaagtaag atggcgctgg ggatgaagaa
    10261 ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc atgataataa ttcttagtat
    10321 taattcggca atctgattct actccccccc agtacctgat actacccccc agtagattaa
    10381 tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt
    10441 gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag
    10501 aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc tgctatccgt
    10561 ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga acacatcgtt
    10621 caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa ggtgctggat
    10681 tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct cgtttacaac
    10741 gggtgcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa acgactcggg
    10801 gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat aatgccagcg
    10861 tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga gcaaactatg
    10921 caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt tcaccaacat
    10981 tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc caataccttg
    11041 ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag tcagtttgcg
    11101 gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg cgcacaggcg
    11161 atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct tgatccagta
    11221 gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc ttttaaaccg
    11281 gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc taatggcgga
    11341 cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca aacactggca
    11401 cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac cgatggacat
    11461 cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg aactggctgt
    11521 gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc
    11581 gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg
    11641 ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca ggaggtgcag
    11701 gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt ggtgcgggga
    11761 ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca gttattactg
    11821 cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag cctgattttg
    11881 tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact aaaatcggta
    11941 tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc
    12001 aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg ctttcacctt
    12061 cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata acgccaaact
    12121 tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg
    12181 aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc
    12241 tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa cggtttaccg
    12301 caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct gcggcgttgg
    12361 ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca aaaagctggc
    12421 tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt ggtccggttc
    12481 accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat tgtcagcctt
    12541 tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc
    12601 accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc gcgtcagacg
    12661 gtgaattttt tctattcgac agcagttcag attacagact acatccactt ctacggttat
    12721 cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca cggattagat
    12781 gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat
    12841 atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt ggacccctat
    12901 ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc gtatcattat
    12961 ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac ggtggatttc
    13021 tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa
    13081 gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac gggaaaaaag
    13141 ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta tttcaatgaa
    13201 tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg catggcctgg
    13261 cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt
    13321 aatgtgttta atggcacggt ggatgagttg caggcattcg ttgatgggat taaagaaacg
    13381 ccttaaataa gaagcagtga tatactccca ccagactccc tggcaggaaa aagacaaatg
    13441 gaacaagcgc atacccagct gattgcccaa ctgaacgagc gtattttagc ggcggataac
    13501 acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg ccgtgcgcag cggggtgctg
    13561 gagcatggca atattttgcc cggtgagcgt gatttaagtc agttaaccgg cgtttcgcgc
    13621 attacggtgc gcaaggcgat gcaggcgctg gaagaagagg gcgtggtgac gcgttcgcgc
    13681 ggttacggca cgcagatcaa caacatcttc gaatactcgt tgaaagaagc gagaggtttt
    13741 tctcagcagg tggtattgcg cgggaaaaag cccgatacgc tatgggttaa taagcgtgtc
    13801 gtgaaatgtc ccgaagaagt tgcgcagcag ctggcggtcg aagcgggaag tgatgtcttt
    13861 ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat cgattgagga atcgtgggtt
    13921 ccggcgcatt taattcatga tgttgatgcc atcggaattt ctctttatga ctatttccgc
    13981 agccagcaca tttacccaca acgtacgcgt tcccgcgtta gcgcccggat gccggatgcc
    14041 gagtttcagt cacatattca gttagatagc aaaataccgg tgctggtgat caagcaagtg
    14101 gcgcttgacc aacagcaacg gcctattgag tacagcatta gccactgtcg cagcgatcta
    14161 tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc gcacaatcac cgccccgatg
    14221 cccgacaacc cacgacgcca ctgcattgcc cagtaatacg gcatccgcca gtggcagacc
    14281 agaggccagc ccggcaagta cgccacctgc atgactgtcg cccgccccaa tggtgtctac
    14341 aacttgcgtt ggaaatgccc gaatgcagcc agaagcgtcg ttgctgaaat accatgcgcc
    14401 ttctttatcg agacgaacga tcaacggcgc ggcaaatttc tcctgccatt gcttgccaag
    14461 tgttgttatc tctgcagata aagcaaaacg ttcggcggca atctcagcct cttgacgatt
    14521 gagtgacact aaaggtcgac aggccatgat ccgcgccagt aatgcgtccg ggatatcgcc
    14581 aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc agcttttcca gccattccac
    14641 taacaattcg ccgcagggcg aggccagttg ataaccggaa aaatagagca ggttgccagg
    14701 cgcaacggtt aatcgtgcca accattggcg attccattga ttttccacac cgctgaatga
    14761 cataaaagtg cgttcgccat ccggctcaac cagtgccaga caccagccgt tatcgctttc
    14821 ggcgttatcg atcaggctga ttaacccctc tttcgccatc cggttgcgaa taatctccgc
    14881 ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaacgc
    14941 cacggcaata ttcagtgcgc agccgccaac gttaacgctc tgctgtttca gttcgatatc
    15001 gcacccacgc caggggaggg cataagcgtc ggcgatcacg tcaatgaccg ccgcgccgac
    15061 gaccatcacc ggctgacgcg tggttaattc aggcagcagt gtgtgtaatc gagcgccgct
    15121 cataccgcct cccgttgttg acgatatttc tccagcgctg tggcatagcg gttgaaatca
    15181 agctgattta ccgcatccag ctccgccttt aacgcaggat cgacagcgtt aatgccatgc
    15241 aacgcgccgc aaatcgccgt cgccatagca cctatggtgt ctgtgtcgcc gccaagattg
    15301 gcgcacagga cggcgcagcg gtttgggtcg gtttgtgcca gttcaactag cgcaatggcg
    15361 cacggaacgg actcaatagt gctggtgcct gcgccaacga cctggtaaag ctgttcgctg
    15421 gcggattcgg tgccgtcggc attacgcaca attttcagcg caatctccag acgtgccgcc
    15481 agtgaggcgc tgaacgtggt ggtgcgtttt tgctgtgcat gtcgcgcaat tgaaggcaat
    15541 gaatcaacaa tcgctgacca gcgttctccg tcaatggcac gagaaatcgc ccatgcgatg
    15601 actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg ggctggacgc cagcgccaca
    15661 tcatcaataa aggaatcaac atcacgcgct ggaagcaaac aacctaatgg cgagacgcgc
    15721 atcgctgcgc cgttggttac gccgttgttt tccagttcag caacgggttt accgtcgcga
    15781 atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt ttttgttaaa ggcgtcgaaa
    15841 cgcaacgccc agtcgagaat attacgccca atcagattcg gatcgatctt gccttcacat
    15901 tccagtaacg catccgccag acacaggcca tcgaggtatc gtcggtgaat tcggcgcggt
    15961 taaaataaca ggccgcgtta ttctcctttg gtccaggcag aaaacggtca atccagccaa
    16021 agtgtgcttt aacgcggctg cgtggccaaa gctcggaggg catccccatc gcatccccta
    16081 acgcctgccc ataaagagca ccgagaatac gttctgtttt catttaactt ccccttgtgt
    16141 caacgcaata tctcgatcat cgaccttgat agccgtaatt tcgttgtcgg attcgcggga
    16201 aaaaatcatg aacagcacgg cgataatggc aatcatcacc gcgccgaaag tccacatccc
    16261 ggaccagttg aaagtcagtc cgtttaccgg ttcctgataa gcgaacatct tttccgtcat
    16321 cacaccgcca agacgatagc cgagcaaact gccgaagccc tggcagcaga gcgtgatcag
    16381 cccctgcgca gcggtacgca tatgcacggg ggctttttta tcgacataga tgtaagcggt
    16441 aacgtagtaa aaatcgtaac ttacgccgtg aagcaaaata ccgaggaaca gtaacgcgta
    16501 ggtgaaattg acaggtaatg taaggtacgc gatgacaata ccacataata ataccttttg
    16561 aatacctttg cggtaaaaac ttgaccgcgc taacattttt tctgatgaat cgagccaaca
    16621 gaaaacgctg aaaaaacatc caaaagatgg aaaaactcgc ccgtaacgac caggattatg
    16681 attgcccgca aaaggcgggc caggacaatc catagccgat atccaatcgg aatttacggg
    16741 agcatagtaa tgacagatat tgcgcagttg cttggcaaag acgccgacaa ccttttacag
    16801 caccgttgta tgactattcc ttctgaccag ctttatctcc ccggacatga ctacgtagac
    16861 cgcgtgatga ttgacaataa tcgcccgcca gcggtgttac gtaatatgca gacgttgtac
    16921 aacaccgggc gtctggctgg cacaggatat ctttctattc tgccggttga ccagggcgtt
    16981 gagcactctg ccggagcttc atttgctgct aacccgctct actttgaccc gaaaaacatt
    17041 gttgaactgg cgatcgaagc gggctgtaac tgtgtggcat caacttacgg cgtgttggtg
    17101 tcggtatcgc ggcgctatgc gcatcgcatt ccatttctcg tcaaacttaa tcacaacgag
    17161 acgctaagtt acccgaatac ctacgatcaa acgctgtatg ccagcgtgga gcaggccttc
    17221 aacatgggcg cggtggcggt tggtgcgact atctattttg gctcggaaga gtcacgtcgc
    17281 cagattgaag aaatttctgc ggcctttgaa cgcgcgcacg agctgggcat ggtggcagtg
    17341 ctgtgggcct atttgcgaaa ctccgccttt aagaaagatg gcgttgatta ccatgtttcc
    17401 gccgacctga ctggtcaggc gaaccatctg gcggcaacca taggtgcaga tatcgtcaaa
    17461 caaaaaatgg cggaaaataa cggcggctat aaagcgatta attacggtta taccgacgat
    17521 cgcgtgtaca gcaagttaac cagcgaaaac ccgattgatc tggtgcgtta tcagttagct
    17581 aactgctata tgggccgggc tgggttgata aactccggcg gtgctgcagg cggtgaaact
    17641 gacctcagcg atgcagtgcg tactgcggtt atcaacaaac gcgcaggcgg aatggggctg
    17701 attcttggac gtaaggcgtt caagaaatcg atggctgacg gcgtgaaact gattaatgcc
    17761 gtgcaggacg tttatctcga tagcaaaatt actattgcct gatacgcctc atacgaacag
    17821 caccaccccg taggacggat aaggcgttta cactgcatcc ggcaacctat gcctgatgcg
    17881 acgcggaagc gtcttatcat gcctacatag cactgccacg tatgtttaca ccgcatccgg
    17941 cataaaaaca cgcgcacttt gctacggctt ccctatcggg aggccgtttt tttgcctttc
    18001 actcctcgaa caattttcat attgtcgttt ttgtgatcgt tatctcgaca tttaaaagca
    18061 aataatttca ttattttttg aaatcgaaag caaacaacaa gatatgaaaa tgtacgtggt
    18121 atcgacaaag cagatgctga acaacgcaca gcgcggcggt tatgcggttc cggcattcaa
    18181 tattcacaat ctcgaaacga tgcaagtggt ggtggaaacc gctgccaacc tgcatgcgcc
    18241 ggtcatcatc gccggaacgc ctggcacatt tactcatgct ggtacagaaa atctgttggc
    18301 gctggtcagc gcgatggcga agcaatatca ccatccactg gcaattcatc tcgaccatca
    18361 cacgaaattt gacgatatcg ctcagaaggt tcgttctggc gtgcgctcag tcatgattga
    18421 cgcctcgcat ttgccttttg cgcaaaatat ttcacgggtc aaagaggtgg tggatttttg
    18481 ccatcgcttt gatgtcagcg tcgaggcgga gctggggcaa cttggcggcc aggaagatga
    18541 tgtgcaagtc aatgaagccg atgcgttgta caccaacccc gcacaggcgc gtgaatttgc
    18601 cgaggcaacc gggattgatt ccctggcggt cgccatcggc acggctcacg gtatgtatgc
    18661 cagcgcaccg gtgcttgatt tttctcgact ggagaacatt cgccagtggg tgaacttacc
    18721 gctggtgctg catggcgcgt caggattatc gactaaggat attcagcaaa ccatcaaact
    18781 ggggatatgc aaaatcaacg ttgcaacgga gctgaaaaat gccttctcgc agtcgttaaa
    18841 aaattacctg accgagcacc ccgaagcgac cgatccccgg gattatttgc agtcggctaa
    18901 atccgcaatg cgcgatgtgg tgagcaaagt gattgccgat tgtggctgcg agggcagggc
    18961 ataacgcacc tgccatttaa caaggaaaaa acatgaaaac gttaattgcc cggcataaag
    19021 ctggtgaaca tatcggcata tgttcagtct gttctgccca tccgttggtt atcgaagcgg
    19081 cgctggcatt tgatcgcaac agcacgcgca aagtgctgat tgaagcaacg tcaaaccagg
    19141 tcaatcaatt tggcggttat accggaatga cactggcaga ctttcgcgaa tttgttttta
    19201 cgattgccga taaagttggg tttgcacgcg aacgcattat tctcggcggc gatcatctgg
    19261 ggccaaactg ctggcagcaa gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg
    19321 taaaagcata tgttcgtgct ggcttcagta aaattcatct tgatgcgtca atgtcctgcg
    19381 cgggggatcc cataccgtta gcaccagaaa cggttgcgga acgagctgct gtgctttgct
    19441 ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat
    19501 tggcactgaa gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac
    19561 ccatgttgaa gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg
    19621 gctgacagag gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga
    19681 tcacagcaat attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga
    19741 aaacacccga atggtttatg aagcacattc taccgattac cagacccgga cggcttattg
    19801 ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt cctgcattaa cctttgcttt
    19861 acgcgaggcg atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg
    19921 cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa
    19981 atattatcgc acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg
    20041 tattcgttat tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa
    20101 tctgcaaggc gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga
    20161 acgcattcag tccggggaat tatcagcaat gccgcatcag cttattatga ataaaattta
    20221 tgatgttttg cgcgcctatc gctacggttg tgcggaataa ggacggtata tgactaacct
    20281 gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa
    20341 tgagatgctc gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga
    20401 agcagaattc cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga
    20461 ggcgattcat gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt
    20521 tcagcaagcg gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga
    20581 aaatccgcag cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga
    20641 tatcgtcgag acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta
    20701 tgttttagat tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg
    20761 caagattatt gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat
    20821 taaagagttg tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga
    20881 aatagaaacc tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag
    20941 ttttggcgat attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc
    21001 attacaaaat aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg
    21061 ttatattctc gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa
    21121 aatattaggc atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt
    21181 tgttggcatt ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc
    21241 gatggcggaa aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc
    21301 accaatgacc tggacgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa
    21361 cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg
    21421 gcatatgacc ttcaccggca gcgttgctgc acctggcaac cggttcatgg atgataggga
    21481 tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
    21541 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
    21601 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
    21661 ttaaatttag cgccgacgat attcagcgca aatttggccc ttttggcgaa cctgtcaccg
    21721 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
    21781 tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacggatg attaaaccca
    21841 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
    21901 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
    21961 tatcggcaag cctgattttt attccgctca ccattttaat tgctgtttgt gttccgggta
    22021 atcaggtact gccgtttggc gatcttgcca ctatcggctt cttcgtggcg atggcggtcg
    22081 cggtgc
//
LOCUS       DAJTYI010000059        21664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYI010000059 DAJTYI010000000
VERSION     DAJTYI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..826)
                     /locus_tag="OO564_002178"
     CDS             complement(<1..826)
                     /locus_tag="OO564_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6653295.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPL"
     gene            complement(864..2144)
                     /gene="puuB"
                     /locus_tag="OO564_002179"
     CDS             complement(864..2144)
                     /gene="puuB"
                     /locus_tag="OO564_002179"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6653296.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(2146..3634)
                     /gene="puuC"
                     /locus_tag="OO564_002180"
                     /pseudo
     CDS             complement(2146..3634)
                     /gene="puuC"
                     /locus_tag="OO564_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(3784..4341)
                     /gene="puuR"
                     /locus_tag="OO564_002181"
     CDS             complement(3784..4341)
                     /gene="puuR"
                     /locus_tag="OO564_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6653297.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(4368..5132)
                     /gene="puuD"
                     /locus_tag="OO564_002182"
     CDS             complement(4368..5132)
                     /gene="puuD"
                     /locus_tag="OO564_002182"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6653298.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            5344..6762
                     /gene="puuA"
                     /locus_tag="OO564_002183"
     CDS             5344..6762
                     /gene="puuA"
                     /locus_tag="OO564_002183"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6653299.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            6911..7075
                     /locus_tag="OO564_002184"
     CDS             6911..7075
                     /locus_tag="OO564_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653300.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            7065..8450
                     /gene="puuP"
                     /locus_tag="OO564_002185"
     CDS             7065..8450
                     /gene="puuP"
                     /locus_tag="OO564_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6653301.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            8584..8829
                     /locus_tag="OO564_002186"
     CDS             8584..8829
                     /locus_tag="OO564_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6653302.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            9142..10785
                     /gene="sapA"
                     /locus_tag="OO564_002187"
     CDS             9142..10785
                     /gene="sapA"
                     /locus_tag="OO564_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6653303.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            10782..11747
                     /gene="sapB"
                     /locus_tag="OO564_002188"
     CDS             10782..11747
                     /gene="sapB"
                     /locus_tag="OO564_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6653304.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            11734..12624
                     /gene="sapC"
                     /locus_tag="OO564_002189"
     CDS             11734..12624
                     /gene="sapC"
                     /locus_tag="OO564_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6653305.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            12624..13616
                     /gene="sapD"
                     /locus_tag="OO564_002190"
     CDS             12624..13616
                     /gene="sapD"
                     /locus_tag="OO564_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6653306.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            13618..14424
                     /gene="sapF"
                     /locus_tag="OO564_002191"
     CDS             13618..14424
                     /gene="sapF"
                     /locus_tag="OO564_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6653307.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            14492..14845
                     /locus_tag="OO564_002192"
     CDS             14492..14845
                     /locus_tag="OO564_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6653308.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            15213..16001
                     /gene="fabI"
                     /locus_tag="OO564_002193"
     CDS             15213..16001
                     /gene="fabI"
                     /locus_tag="OO564_002193"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6653309.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            16147..17274
                     /locus_tag="OO564_002194"
     CDS             16147..17274
                     /locus_tag="OO564_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6653310.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            17342..19276
                     /locus_tag="OO564_002195"
     CDS             17342..19276
                     /locus_tag="OO564_002195"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6653311.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(19354..19456)
                     /locus_tag="OO564_002196"
                     /pseudo
     CDS             complement(19354..19456)
                     /locus_tag="OO564_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19510..21484
                     /gene="pdeR"
                     /locus_tag="OO564_002197"
                     /pseudo
     CDS             19510..21484
                     /gene="pdeR"
                     /locus_tag="OO564_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         4913 a         5666 c         5686 g         5399 t
ORIGIN      
        1 aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat taagtccggc ttatcggcgt
       61 aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc gctttgcact tcatcggcaa
      121 tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc ggcaaccagc tcttttggcg
      181 caacgttgaa accgccctcg ccctgcaccg gttcgaaaat aatggccgcg acctgcttcg
      241 cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag ggagtcctgt gttgaaatgc
      301 cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc agggaacggg ccgaagccga
      361 ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat atatgtacga ccgtggaagc
      421 caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc gcgagcaatt ttcaccgcgt
      481 tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt agcctgcccg ctcactgggg
      541 caagggcgtt aattttctcc gccagcgtga cgtagctttc gtacggcaca atctgatacg
      601 ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc gaccagatca gagtggcgat
      661 gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat gtattcgttg ccctctacgt
      721 ccttcagcgt agcgttttct gccgactggg cgaagaagtt acacatcacg ccaaccccgc
      781 gtggggtagc agaaagacga cgctgatgga attcattgtt gctcatgttt cacacctttt
      841 gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct gtaataagcc
      901 gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg gtaatgcggc
      961 agattggcga aggcatcgaa acgctcggcg tcaccgcgca gcagttcagc aatcaaacgc
     1021 ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac cctgcatgta atagatgttg
     1081 gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca gcaggaagtt gcccgtccag
     1141 cggtaatcaa ttttcacgcc cttcaactga gggaaggttt ttagtagttt cggtaccaca
     1201 agacgctcaa cgtcatccgg gttgcgtgca ccatacacta cacccccgcc gtacagcagg
     1261 cggttgtcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc cacacagtag
     1321 tttttcggga tcagcgaacg ggctaaatct tccgacagcc gttcggtggt gatcacctgc
     1381 gtgccgcacg gcatgctgcg tttcgctagt tccggctcta ctttatcgcc cagatacgca
     1441 ttcccggcga caatcacata cttcgccgtc acctgaccat tggcagtccg aaccacggct
     1501 ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat acacgcgccc gccgttcagg
     1561 cggatggcat cggcttcacc aatggcgagg tttagcgggt gaatatgccc accgctgtga
     1621 tccagcagcg caccagtgta gcgatcgctg gcgacctcgc ggcgaatagc gttggcgtcc
     1681 agcagctcca gctgtttatt gccgtagcgt tcccagttct ctttctgctc ttcgagtgtg
     1741 gcgagttgtt tatcattcat cgccacaaac agaccgccgg ggcggtagtc gcagtcaatc
     1801 tgataacgtt tgatacgttc gcggatgatc tcgccaccct cgaacatcat gctgccgagc
     1861 atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt cgatgtcgcg gctgtaggag
     1921 ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc cgatgcgtga ggcttcgaga
     1981 accactacgt caaagcccgc ttccgccaga tgcagcgcgg aggagagccc ggtatagccg
     2041 ccgccaacca cgcaaacgtc gcaggtgatt gactcattca gtgtatcgaa tggtgcatat
     2101 ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg cctccaggct
     2161 tatccagatg gttttcagtt cagtgaattt ttcaagggca tgcagggatt tgtcgcgacc
     2221 gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt tgtagttatt
     2281 gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg tgcgcgcggg agaggtcgcg
     2341 cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg gcaagctgca gcgcctgatc
     2401 ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa atctcttcgc ggcttaagga
     2461 cgcattcggg tccacatcca cgaagatggt cgggccgatg gccgctgcca gcccggcgtt
     2521 gcgaccatcc agcagcagtt gccccttgcc ttcgccttca cgaataaagg tgtgtaccga
     2581 gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt gcgggatcaa gtggatggcc
     2641 cggctgccag ttttgcgcct gctgttttaa cagggctaag aattcatcgg cgatgctctc
     2701 ttccagtaac aaacgcgttc cggcgatgca cacctggccc tggttgtaga agatgcctgc
     2761 ggcggtggcg cttgccgctt tttgcagatc cggacaatcg gcgaaaacga tgttggcgct
     2821 tttgccgccc gcttccagcc agacacgttt catgttgcta tcacccgcgt ctttcagcag
     2881 ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca tcgatatcgt tatgacgcga
     2941 aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg ttcaacacac catccggcaa
     3001 gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg agcggtgatt tttcagacgg
     3061 ttttagaatc acgctgtttc ccgccgccag cgccgggccg agtttccagc aagtcagcaa
     3121 cagcgggaag ttccacggca cgatggcggc aatcacgccg accggttcac gcacgatcat
     3181 cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga tcgcttcggc
     3241 gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcagactgt gacgaatccg
     3301 gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat
     3361 cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac cagtcgccgc
     3421 gttcaaatac gccgcgagct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg
     3481 caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg
     3541 ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgagg cttaacgctt
     3601 tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag
     3661 atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc ccgtttattt
     3721 ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
     3781 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
     3841 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
     3901 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
     3961 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
     4021 cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt gacaccccct gactgcccat
     4081 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
     4141 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
     4201 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
     4261 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
     4321 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
     4381 ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat acgcgaaagc
     4441 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
     4501 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
     4561 ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt
     4621 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
     4681 tgttccaccg gcagttcggg atcttcccga tgctccagca attcaggctg ttcgcacagc
     4741 ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg gcagatggcg
     4801 aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag aagatcacgc
     4861 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
     4921 ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taacgacggt
     4981 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
     5041 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
     5101 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
     5161 tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc tagcagtgga
     5221 ttttcatttt tgcaaactaa atttaacatt tgacaaacat ttggtttgca tacagattcg
     5281 aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
     5341 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
     5401 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
     5461 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
     5521 agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc aatggatatt
     5581 ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc
     5641 tgtgttcctg ttctcggttc cttaactcct tctgccgccg atccagagtt tatcggtcag
     5701 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
     5761 cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggttgt agcggtagag
     5821 ctggagttct atttactgga tcgccagcgc gacgctgaag gggatctgca accgccctgc
     5881 gcgcctggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
     5941 cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttgcagct gattccggca
     6001 gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta ccatactgat
     6061 aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg tctgatggca
     6121 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
     6181 gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca
     6241 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
     6301 tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
     6361 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg
     6421 tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga tgccaaccca
     6481 tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg
     6541 ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
     6601 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
     6661 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
     6721 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattcccctt gcggggttgt
     6781 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
     6841 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacaagg
     6901 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
     6961 tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
     7021 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
     7081 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
     7141 aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
     7201 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
     7261 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
     7321 cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat
     7381 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
     7441 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
     7501 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
     7561 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
     7621 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
     7681 cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca
     7741 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
     7801 cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
     7861 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg
     7921 gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc
     7981 tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
     8041 cagagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta
     8101 tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga
     8161 ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct
     8221 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
     8281 tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac
     8341 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtacctgatc cgccgctatc
     8401 gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg
     8461 cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat
     8521 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
     8581 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
     8641 tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg
     8701 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
     8761 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
     8821 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca
     8881 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
     8941 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
     9001 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
     9061 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
     9121 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
     9181 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
     9241 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
     9301 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
     9361 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
     9421 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
     9481 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
     9541 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
     9601 gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc
     9661 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
     9721 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
     9781 ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
     9841 catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc
     9901 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
     9961 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    10021 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    10081 gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    10141 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    10201 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    10261 gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    10321 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttgacgtaaa agtggtgatt
    10381 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    10441 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc gttactgagc
    10501 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    10561 gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa
    10621 gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc attgcgtttg
    10681 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    10741 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    10801 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    10861 gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    10921 ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg
    10981 ccgaacagtt aaaagaggtc ttcccggcga cgatagaact gtgcatcctc gccttcggtt
    11041 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    11101 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    11161 cgcttctgtt gacactgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    11221 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    11281 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    11341 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta
    11401 ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta
    11461 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    11521 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    11581 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    11641 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    11701 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    11761 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    11821 atggtcggcc tgtacggctg cgcgggactg gctgtactgt gtatttttgg cggctggttt
    11881 gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc atcatggtcg
    11941 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgtga tgttttaagc
    12001 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    12061 acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg
    12121 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc
    12181 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    12241 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    12301 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    12361 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    12421 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    12481 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    12541 ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt
    12601 gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    12661 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    12721 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    12781 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    12841 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    12901 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    12961 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    13021 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    13081 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    13141 tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg gagccaacaa
    13201 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt
    13261 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    13321 actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc
    13381 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    13441 gtcgcctcaa tactctaccc ggcgctatcc cattgctgga acagttaccg attggttgtc
    13501 gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    13561 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    13621 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggttgg
    13681 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    13741 acactggcga ttattggcga gaatggttcg ggtaaatcca cactggcaaa aatgctggcg
    13801 ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct gcattttggc
    13861 gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    13921 aatccccgtc aacgtatctc acaaattctg gattttccac tgcgcctgaa caccgacctg
    13981 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    14041 gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg
    14101 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    14161 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc
    14221 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    14281 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg
    14341 ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg
    14401 gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat tcacctcatt
    14461 ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg
    14521 tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg
    14581 acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt
    14641 tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt
    14701 tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt
    14761 ctggaataac gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact
    14821 gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt
    14881 gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc
    14941 atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg
    15001 cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgccccc
    15061 cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt
    15121 ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg
    15181 cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc
    15241 tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag gcgatgcacc
    15301 gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag
    15361 aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca
    15421 gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac
    15481 actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc
    15541 gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag
    15601 cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg
    15661 agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg
    15721 tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg
    15781 gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt
    15841 gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt aactctgcgg
    15901 cattcctgtg ctccgatctc tctgccggta tctccggtga agtagtccac gttgacggcg
    15961 gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt tggtaaagat
    16021 gggcggcgtt ctgccgcccg cgttatctct gttatacctt tctgatattt gttatcgccg
    16081 atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta cgaccaagga
    16141 gcgcccatgg aacaacgcca catcaccggc aaaagccact ggtatcatga aacgcaatcc
    16201 agtactacgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc
    16261 tttctgctcg acgtgatcct tgatgaagag acgctggccc ccttcctttc atggctggtc
    16321 cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc ttaccgtgac ccgttcacaa
    16381 accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt ttgcggcgtc
    16441 cagcggttat gtaactacta ttcggcgcga cttacgccgc tccccggacc tgattcctcc
    16501 agggaaaata atcatcggtt ggcacaaatc acgcaatatg cccgccaact ggctagctcg
    16561 cctgctatta tcgacaaccg atcgcgccag catctgaatg acgtcggtct tactgtctgg
    16621 gactgtgtga tcattaacca aatcattggt tttattggct ttcaggcgcg gacaattgcg
    16681 acatttcagg cttatctcgg gcatccggta cgctggttac ccgggctggc gatacaaaac
    16741 tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg
    16801 gaaaaactac ctgaagagca cacaaaaagt tcaactgcag aactttgcca actggccgaa
    16861 acacactctc tccaccctat ttcactttcc cttctcgaaa agttgttaaa cagcacacgg
    16921 gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcgtat aaatggcagt
    16981 cctgcttgtt tttccacctg tatggattca tcaaatgaat ataaaaaaat cagcaccctt
    17041 ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc gtcattctgt tgagcgcgct
    17101 accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc
    17161 agccctttac tcgaacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc
    17221 gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc
    17281 cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat
    17341 tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc
    17401 acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc
    17461 acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat
    17521 tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga
    17581 accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tgaccatcgt
    17641 tcctgatcat ccacttttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca
    17701 cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga
    17761 tcgttctttc tatgctgaac tgacacaata catcactttt ggcgacgacc actttgtacc
    17821 gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac
    17881 cgaaatgctc gatgaaggtc tggttcgtga agatctgacc tcgctggatt ttgtcaccat
    17941 cgacagtgcc agcactgaag atatggatga cgcccttttc gctaaggcgc tgccggatga
    18001 caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa
    18061 gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc
    18121 tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc
    18181 ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt
    18241 ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gaccaggttt ctgactggct
    18301 ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gccgaacaag tccgtttgct
    18361 agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga
    18421 tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga
    18481 gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc
    18541 ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga
    18601 tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc
    18661 cgaagaagta ctcacgctgg acggtttctg caaactgcgt cgtgaactgg atgcgcaacc
    18721 aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga
    18781 acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg
    18841 taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc
    18901 gacgcgtccg caggatgaaa tcactgtcca aatggccgaa cgtcgccgtc tcaaccggat
    18961 ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagata aagccgggac
    19021 cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc gtgtgcgttt
    19081 ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg tgcgcgatga
    19141 actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt
    19201 aactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca gcattattgc
    19261 gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc
    19321 ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt gaaaactggc
    19381 gtttttccaa aactggatta caaataaata tataaaaaac aatgcgttta tccgccgtta
    19441 atttcatgta agtcatgttg ttccttttat ttaaccgcgg actccgctgt taaccggagg
    19501 atatgcatca tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac
    19561 aatccatact ggcggttgac ggaaagcagc gatgctttgc gcttttctac caccaaaacc
    19621 acagaacctg atcgtacatt gcagttatct gccgaacagg ctgctcgcat cagggaaatg
    19681 acggtcatca cctccagcct gatgatgagt ctgaccgtcg atgaaagcga tctttctgtg
    19741 catctggtag gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat
    19801 gacacaccgg cggttgctcg tgatttatca cacgggcttt cctttgctga gcaggtagtt
    19861 tctgaagcac attccgcaat agtgattctc gacagccggg ggaatatcca acgcttcaat
    19921 cggttatgtg aagattacac agggttgaaa gaacacgacg tcattgggca aagcgtgttt
    19981 aaactgttta tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt
    20041 cgaagcggca atgcatatga agtcgaactg tggataccaa cacgtaaagg ccagcggctg
    20101 tttctgtttc gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc
    20161 tgttccggta ccgacattac cgaagagcgc cgcactcagg agcgactgcg tattctggca
    20221 aataccgaca gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt aatcgatcac
    20281 gctattaatc aggcagataa caataaagtt ggggttgtgt atcttgattt ggataatttt
    20341 aaaaaggtca acgacgccta tgggcatttg tttggtgacc agttattacg cgacgtgtca
    20401 ttggctattt taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggcgatgag
    20461 tttctggtac tggcatccaa tacctcacaa agcgcgctgg aagcaatggc atcacgaatt
    20521 ttgacccgct tacgcctccc ctttcgtatt ggtttaattg aagtttatac cagctgttca
    20581 gtaggtattt cgctctctcc cgaacatggt tcggacagcg cgactattat tcgtcacgcc
    20641 gacacagcaa tgtacacagc gaaggaaggc ggacgaggac aattttgcgt ttttactcca
    20701 gaaatgaatc agcgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg
    20761 gaaaacgatc agttggttat cgaaaatcac ctagcgtggc gaagtgcgca gtctggaagc
    20821 actagtacgt tggcagtcac ctgaacgtgg gttgattcca ccgttggact tcatttccta
    20881 cgccgaagag tcagggctaa ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg
    20941 ccaggtggca aagtggcggg ataaaggcat aaacctgcga gtggcggtaa atatttctgc
    21001 acgtcagctc gccgatcaaa ccattttcac cgccctgaaa caggttctcc aggaactcaa
    21061 ttttgaatac tgccctatag atgttgaact gacagagagt tgtctgattg agaatgatga
    21121 actggcactg tctgttattc aacaatttag ccaactaggt gcgcaagtgc atctggatga
    21181 ttttggtacc ggctactctt cactttcgca actggcgcgc tttccgatcg atgccatcaa
    21241 acttgaccag gtttttgttc gagatattca caaacaacct gtctcgcagt cactggtccg
    21301 ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg atcgccgaag gtgtagagag
    21361 tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat gagcggcaag gatttttgtt
    21421 tgccaaaccg atgcccgccg tcgccttcga acgctggtat aaacgctatc tgaagcgcgc
    21481 ataagtagat gaatatatat acttagcgct atatcccgtc attatatatc gtattcctgg
    21541 atgataatta gccgttgctg ttaaacactt ctcttttagc ggcaattttt gcattattca
    21601 ttttccccct tttgctataa ggatgtcctt gatgtctgaa tttgatgccc agcgcgttgc
    21661 cgaa
//
LOCUS       DAJTYI010000060        21508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYI010000060 DAJTYI010000000
VERSION     DAJTYI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..82)
                     /locus_tag="OO564_002198"
     CDS             complement(<1..82)
                     /locus_tag="OO564_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6653312.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIR"
     gene            221..361
                     /locus_tag="OO564_002199"
     CDS             221..361
                     /locus_tag="OO564_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653313.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            375..1247
                     /locus_tag="OO564_002200"
     CDS             375..1247
                     /locus_tag="OO564_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6653314.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1307..2605)
                     /gene="eno"
                     /locus_tag="OO564_002201"
     CDS             complement(1307..2605)
                     /gene="eno"
                     /locus_tag="OO564_002201"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6653315.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2693..4330)
                     /gene="pyrG"
                     /locus_tag="OO564_002202"
     CDS             complement(2693..4330)
                     /gene="pyrG"
                     /locus_tag="OO564_002202"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6653316.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4558..5349)
                     /gene="mazG"
                     /locus_tag="OO564_002203"
     CDS             complement(4558..5349)
                     /gene="mazG"
                     /locus_tag="OO564_002203"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6653317.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5445..7679)
                     /gene="relA"
                     /locus_tag="OO564_002204"
     CDS             complement(5445..7679)
                     /gene="relA"
                     /locus_tag="OO564_002204"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR6653318.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(7727..9028)
                     /gene="rlmD"
                     /locus_tag="OO564_002205"
     CDS             complement(7727..9028)
                     /gene="rlmD"
                     /locus_tag="OO564_002205"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6653319.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9085..11841
                     /gene="barA"
                     /locus_tag="OO564_002206"
     CDS             9085..11841
                     /gene="barA"
                     /locus_tag="OO564_002206"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6653320.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12073..13413)
                     /gene="gudD"
                     /locus_tag="OO564_002207"
     CDS             complement(12073..13413)
                     /gene="gudD"
                     /locus_tag="OO564_002207"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6653321.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13434..14774)
                     /locus_tag="OO564_002208"
     CDS             complement(13434..14774)
                     /locus_tag="OO564_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6653322.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKVLNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14776..16128)
                     /gene="gudP"
                     /locus_tag="OO564_002209"
     CDS             complement(14776..16128)
                     /gene="gudP"
                     /locus_tag="OO564_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6653323.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16562..17011)
                     /locus_tag="OO564_002210"
     CDS             complement(16562..17011)
                     /locus_tag="OO564_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6653324.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17029..17811)
                     /gene="truC"
                     /locus_tag="OO564_002211"
     CDS             complement(17029..17811)
                     /gene="truC"
                     /locus_tag="OO564_002211"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6653325.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17811..18140)
                     /locus_tag="OO564_002212"
     CDS             complement(17811..18140)
                     /locus_tag="OO564_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6653326.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18762..19307)
                     /gene="syd"
                     /locus_tag="OO564_002213"
     CDS             complement(18762..19307)
                     /gene="syd"
                     /locus_tag="OO564_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6653327.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19375..20223
                     /gene="queF"
                     /locus_tag="OO564_002214"
     CDS             19375..20223
                     /gene="queF"
                     /locus_tag="OO564_002214"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6653328.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20335..>21508
                     /gene="ppnN"
                     /locus_tag="OO564_002215"
                     /gene_synonym="ygdH"
     CDS             20335..>21508
                     /gene="ppnN"
                     /locus_tag="OO564_002215"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6653329.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRA"
BASE COUNT         5325 a         5694 c         5105 g         5384 t
ORIGIN      
        1 aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg gtctggaaca
       61 tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt aattattgca
      121 gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt taaagaaaaa
      181 tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag aaaataaaga
      241 aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt ctgtattagt
      301 ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta tccagattta
      361 atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt tcctttgttg
      421 cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt acgagtagct
      481 taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc aataataccc
      541 aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt gaacaatatg
      601 ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat gggatactga
      661 tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg ctggaagaaa
      721 aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc gcatttaaac
      781 aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac caactcactt
      841 cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc actgatcatt
      901 attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg ttttttcatc
      961 aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca atccatcttt
     1021 tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc ttctttttta
     1081 ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga gcatctggta
     1141 gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc ggatttagcg
     1201 gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc caccataaaa
     1261 aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat gcctggcctt
     1321 tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca
     1381 gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg
     1441 cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca gaacggtgag
     1501 agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca
     1561 gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt
     1621 ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc
     1681 ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga
     1741 tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg
     1801 tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag
     1861 ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag
     1921 cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt
     1981 cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa
     2041 cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga
     2101 attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg
     2161 gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc tcgtacagcg
     2221 gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt
     2281 tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc
     2341 cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag
     2401 caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca
     2461 gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac
     2521 cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac
     2581 cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact
     2641 ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt acttacttcg
     2701 cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat
     2761 cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg
     2821 gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg cgcagacctg
     2881 catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa
     2941 caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac tggcactgct
     3001 gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc
     3061 cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa
     3121 attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca
     3181 cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg
     3241 tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca
     3301 gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt
     3361 tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag
     3421 catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt accggattcg
     3481 cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag tttaagctga
     3541 atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga attttataga
     3601 tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa
     3661 tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg atgtcaggct
     3721 ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac
     3781 cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa
     3841 tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata tcacctactg
     3901 taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt
     3961 tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc
     4021 gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca
     4081 ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca gcgccgtctt
     4141 cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt
     4201 aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg
     4261 aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt
     4321 tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaagacggg
     4381 aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca
     4441 gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca
     4501 aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt aattccctta
     4561 gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac
     4621 acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc
     4681 gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag
     4741 attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac
     4801 agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg
     4861 gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg
     4921 cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc
     4981 gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga
     5041 actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat
     5101 atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa
     5161 tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag
     5221 cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc
     5281 ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacggtcgat
     5341 ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc ttcgagtaaa
     5401 agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact cccgtacaac
     5461 cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg ccccagcact
     5521 tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta
     5581 cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt gatatcacgt
     5641 aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc ggcggagtag
     5701 ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag ttccgccagt
     5761 tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa gccgacaatc
     5821 tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt accaacacct
     5881 tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct tttttgctga
     5941 agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa ttgcgattgc
     6001 aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc cagcaactcg
     6061 tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt caggctgatc
     6121 cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg
     6181 tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt gacgtaaccg
     6241 aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat aatttcaatc
     6301 tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg
     6361 atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg cgttgatccc
     6421 gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc gtcaaacacc
     6481 tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg ccacgcaatc
     6541 agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc agctgccgcg
     6601 ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg catctgtttg
     6661 gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt atgaatagac
     6721 tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg caggtggcga
     6781 tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc ggcgacaata
     6841 cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg cattttgcgc
     6901 cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc ttcagctttc
     6961 atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc gagacggcgt
     7021 tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa
     7081 tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg cgcgtagata
     7141 ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt tacttcgcgc
     7201 agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc gaccatcgcc
     7261 aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct
     7321 ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac aaccgactta
     7381 ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag agggaacagc
     7441 agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc caccatctca
     7501 acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg cagacaatac
     7561 gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag acttgcgatc
     7621 catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac cgcaaccatc
     7681 gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat ttaacgcgcg
     7741 agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc atcgccagtc
     7801 gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc gttgcagggt
     7861 tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc ataacacctg
     7921 cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc gcccacggct
     7981 gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt aagccattaa
     8041 gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca ccgaccacac
     8101 tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac agatccagta
     8161 cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt ttttggttca
     8221 cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt
     8281 cataccaagg catctcacca gagacggttt cgagtatctc actatcgggg gcgagataca
     8341 gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg
     8401 gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac
     8461 caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc agcaatgctt
     8521 caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg gagcccgctt
     8581 tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc
     8641 ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt ttcattaatc
     8701 gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt tgttgctgac
     8761 agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg ctatcgctta
     8821 accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca gcaacttccg
     8881 cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt cgcgccacgc
     8941 cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc
     9001 gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc
     9061 ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg catgatgatt
     9121 ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt cgtcgtgcat
     9181 cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat tgagccgctt
     9241 gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg tcagttaata
     9301 agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta tgatgaaaat
     9361 aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca gctcggcagc
     9421 aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat gatcctccgc
     9481 acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga tgccaaaaat
     9541 agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt tcgcttgcag
     9601 caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg tattgcgctt
     9661 atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat ggtgaatacc
     9721 gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat gctcggcgag
     9781 ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc ttatcacgaa
     9841 gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg
     9901 gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt
     9961 aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa tggtgttatt
    10021 ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat
    10081 acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct cgacttctcg
    10141 aaactggaag caggtaagct gattctggaa agtattccat tcccactacg cagcacgctg
    10201 gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga actgacgctt
    10261 aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt acagcaaatc
    10321 atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat tgatattctg
    10381 gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat tcgggatacc
    10441 ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg acaggctgat
    10501 gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac acaaaaactg
    10561 gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg ttcaactttc
    10621 tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc atccatccag
    10681 tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg
    10741 ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt ctccgcgctg
    10801 cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta
    10861 acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct gatgctggca
    10921 cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat cggcgcgtgt
    10981 ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac
    11041 aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg
    11101 gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga tatggtgcaa
    11161 catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca gatgccgttc
    11221 gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc ctgcgaactc
    11281 atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc gcatgcaatg
    11341 gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc gaaaccgatt
    11401 gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg tatttcgtct
    11461 cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg
    11521 caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat gctgcaaatg
    11581 ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt tggagaaaac
    11641 ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta tagcggcgta
    11701 ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg tactaaagaa
    11761 gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt cgcgcgcgaa
    11821 gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt tttatgacgc
    11881 tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt aacgccttat
    11941 ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc tgatacatag
    12001 cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata gagccggttt
    12061 tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc agccaggaat
    12121 cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct gatacagctc
    12181 atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt tttctggcac
    12241 ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat taccttcctg
    12301 ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg caacatgggt
    12361 aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg tcaggccaaa
    12421 ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat gcggatccgc
    12481 cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc agtcggtggc
    12541 gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca tcacttcacg
    12601 cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg aacctttcag
    12661 gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat cgagcgtaat
    12721 acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt cttccccggc
    12781 cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg ccgcttccgc
    12841 caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat accagtcgca
    12901 tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac cgacgaagaa
    12961 cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca gcgatgccac
    13021 gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc cggtaactac
    13081 atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat cacgatcggc
    13141 aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt tacctaccac
    13201 cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa tttcccctac
    13261 gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga acggtgcgtg
    13321 tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga cctgcatttc
    13381 agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga
    13441 cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcgga
    13501 cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc cttttgtacc
    13561 tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt
    13621 ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat ccagtgggta
    13681 tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat cgccagagag
    13741 atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc
    13801 gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg aatatctacc
    13861 gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat gttagtcgcg
    13921 acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga gaagccctgt
    13981 tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt gcataaagaa
    14041 atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc atccgggaag
    14101 cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac cccgccctta
    14161 agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg cacaacggct
    14221 tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc cggcgtattt
    14281 tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata accgaggacg
    14341 gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac attcagcacc
    14401 ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac gcgcaattca
    14461 aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc tttatgtacc
    14521 tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg aatagcatcg
    14581 accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt atgcccggcg
    14641 ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc accaatattg
    14701 agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat aacaggactg
    14761 gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct tgatatcgcc
    14821 caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat aaatcagcgc
    14881 cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc
    14941 agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct cttttggcgc
    15001 ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga aggccagcgc
    15061 cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga
    15121 cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg
    15181 catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc aaaccgccgg
    15241 aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca ccagataaac
    15301 cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac cgatataaac
    15361 gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca cgctgaacgg
    15421 cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg cggcgatata
    15481 ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga ctttcaacca
    15541 gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg accagcccac
    15601 ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga
    15661 gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg caacaatgcg
    15721 actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gagtaaacag
    15781 ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat
    15841 cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat ccagcaacca
    15901 accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca catagcccat
    15961 tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag aaagcgtagc
    16021 gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc
    16081 attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg ttgcactcct
    16141 gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa tcagaggtga
    16201 cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg tgtttatatc
    16261 tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa
    16321 tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa
    16381 atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga gtatgaaatg
    16441 gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg
    16501 catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc tcgctgacat
    16561 atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt tccggttccg
    16621 ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc tgttcctgca
    16681 acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg tcgccgagcg
    16741 caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt ccctgaaaga
    16801 gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc accagaacat
    16861 acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc gttgctttgt
    16921 ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa tttccgtaca
    16981 tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt aggaacttat
    17041 ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag gaagcagacc
    17101 gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt
    17161 cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta gcattaaccg
    17221 ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat gtttgctatc
    17281 gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt tgcgtccggt
    17341 tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg
    17401 catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc ctttatcttc
    17461 gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat ccaacaccgc
    17521 ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt ggtgctgttc
    17581 aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca acacaccaga
    17641 agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt cacgcacggt
    17701 ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca gccagccgga
    17761 gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca ttagctcgca
    17821 tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc gcgttgcgga
    17881 tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc cggtaacggc
    17941 tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa ccactccagt
    18001 ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg ctggggttca
    18061 tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga
    18121 acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg aaggatagca
    18181 ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc agcattccag
    18241 ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg cggccaatcg
    18301 ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc atcctgaagt
    18361 gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc catcctggag
    18421 gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa
    18481 caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc gttgtctgac
    18541 tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca
    18601 atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt ttgattttaa
    18661 atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga tctcctacag
    18721 agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag aggcttaagt
    18781 tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc
    18841 tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc ttcttccagc
    18901 gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag atgaccaatc
    18961 agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg
    19021 atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa ggtatgaatt
    19081 gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta
    19141 aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca cggtgaagga
    19201 acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca cgcatcgcag
    19261 tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa tttctctctt
    19321 cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg aaacatgtct
    19381 tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac cgattaccgg
    19441 gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg cgacccgctg
    19501 ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct gtatgaactt
    19561 tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga acttgattac
    19621 accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag ttttaaccag
    19681 acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc
    19741 gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg ccagccgata
    19801 ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa ctatgaattc
    19861 actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga gacgctcgtc
    19921 agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg ttcgatccaa
    19981 attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct ggtctcattc
    20041 cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga cctgttacgc
    20101 ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg cggtctggac
    20161 attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact ggttcggcaa
    20221 taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg
    20281 cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt tttcttgatt
    20341 acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt ggatatgctt
    20401 aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact tgccgtactg
    20461 aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga aaatttcgat
    20521 attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc cccggaagag
    20581 gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc ggttctgcga
    20641 gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa cctgaatctc
    20701 gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg
    20761 catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat taacgaaaac
    20821 gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct gaatatctgc
    20881 accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg
    20941 cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat tatcgccgct
    21001 gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga aaaacgtctg
    21061 gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt gggtacggca
    21121 gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga tcaggtttta
    21181 ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact ggacgagttt
    21241 gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc
    21301 gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgtgat
    21361 acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt gcaaatgccg
    21421 tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca acctgttgaa
    21481 gtgctggctg ccgacctgcg ccgtgcgt
//
LOCUS       DAJTYI010000061        21470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYI010000061 DAJTYI010000000
VERSION     DAJTYI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..683
                     /locus_tag="OO564_002216"
     CDS             <1..683
                     /locus_tag="OO564_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6653330.1"
                     /translation="GGDGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVN
                     NAGVNFPRLLVDEKAPAGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIV
                     NVSSESGLEGSEGQSCYAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTP
                     EYEEALAWTRNITVEQLREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTN
                     IAGGKTRG"
     gene            693..1100
                     /locus_tag="OO564_002217"
     CDS             693..1100
                     /locus_tag="OO564_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6653331.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1100..1594
                     /locus_tag="OO564_002218"
     CDS             1100..1594
                     /locus_tag="OO564_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6653332.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1660..2457
                     /locus_tag="OO564_002219"
     CDS             1660..2457
                     /locus_tag="OO564_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6653333.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2468..3256
                     /locus_tag="OO564_002220"
     CDS             2468..3256
                     /locus_tag="OO564_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6653334.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3308..4537
                     /gene="sorE"
                     /locus_tag="OO564_002221"
     CDS             3308..4537
                     /gene="sorE"
                     /locus_tag="OO564_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6653335.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            4919..5608
                     /gene="pepE"
                     /locus_tag="OO564_002222"
     CDS             4919..5608
                     /gene="pepE"
                     /locus_tag="OO564_002222"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6653336.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5699..7330)
                     /locus_tag="OO564_002223"
     CDS             complement(5699..7330)
                     /locus_tag="OO564_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6653337.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7550..11233)
                     /gene="metH"
                     /locus_tag="OO564_002224"
     CDS             complement(7550..11233)
                     /gene="metH"
                     /locus_tag="OO564_002224"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6653338.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11433..12257
                     /gene="iclR"
                     /locus_tag="OO564_002225"
     CDS             11433..12257
                     /gene="iclR"
                     /locus_tag="OO564_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6653339.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12574..14758
                     /gene="espL4"
                     /locus_tag="OO564_002226"
                     /pseudo
     CDS             12574..14758
                     /gene="espL4"
                     /locus_tag="OO564_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(14727..16463)
                     /gene="aceK"
                     /locus_tag="OO564_002227"
     CDS             complement(14727..16463)
                     /gene="aceK"
                     /locus_tag="OO564_002227"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6653340.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16647..17951)
                     /gene="aceA"
                     /locus_tag="OO564_002228"
     CDS             complement(16647..17951)
                     /gene="aceA"
                     /locus_tag="OO564_002228"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6653341.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(17981..19582)
                     /gene="aceB"
                     /locus_tag="OO564_002229"
     CDS             complement(17981..19582)
                     /gene="aceB"
                     /locus_tag="OO564_002229"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6653342.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(19851..20780)
                     /gene="metA"
                     /locus_tag="OO564_002230"
     CDS             complement(19851..20780)
                     /gene="metA"
                     /locus_tag="OO564_002230"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6653343.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            20937..21377
                     /locus_tag="OO564_002231"
                     /pseudo
     CDS             20937..21377
                     /locus_tag="OO564_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5265 a         5527 c         5445 g         5233 t
ORIGIN      
        1 acggtggcga tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt
       61 ctagcacaaa agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg
      121 acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc
      181 ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga
      241 aaggtgtttt tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg
      301 tgattgtgaa tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg
      361 ccgctaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac
      421 acggtatccg tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc
      481 cggaatatga agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag
      541 gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt
      601 ttgtttgtta tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg
      661 cgggcggcaa aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg
      721 gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg
      781 aagccgttga atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt
      841 tagcaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc
      901 cgtggaatgc ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc
      961 tttctctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac
     1021 tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa
     1081 cgacagagga ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca
     1141 cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa
     1201 tgacgaagtt tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg
     1261 aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata
     1321 tcaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg
     1381 ccagggggta aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa
     1441 acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa
     1501 taatcttggt gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat
     1561 cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt
     1621 taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa
     1681 atcattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt
     1741 cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa
     1801 accggaataa tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc
     1861 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
     1921 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag
     1981 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
     2041 gcagaagagg cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
     2101 gcgctgcgcg tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc
     2161 agcaatatgt taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt
     2221 tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg
     2281 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc
     2341 ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa
     2401 gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg
     2461 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
     2521 caacctgcaa caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga
     2581 catgatcccc gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag
     2641 gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga
     2701 aattgatgac ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt
     2761 tggcgatcca ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct
     2821 ggcactttcg ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg
     2881 tctggcgatg aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag
     2941 cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt
     3001 tgtgatgggc gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca
     3061 aacgcatgcc gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact
     3121 ttgccctggt ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa
     3181 aattaacccg gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct
     3241 gggcttcctg tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag
     3301 gtggtttatg aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac
     3361 ctttgacctt cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct
     3421 gtgcctctct tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga
     3481 tgtggcgacc aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg
     3541 taaaaagtgg cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca
     3601 actccccgac cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac
     3661 gcatgtggtt attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga
     3721 aacctatttt gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc
     3781 caactatcat cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg
     3841 cacgctgatc ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca
     3901 tggaccagtt aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta
     3961 tgcgcgcaag cactatccat cagaaccgca aacactgatt cattatctca atgccaccga
     4021 tgcagcattt gatacgttaa tggagctgag tggcggtcac ggcttcgatg atattttcgt
     4081 ctttgtgcct aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg
     4141 cctgaatttc ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga
     4201 tgtgcattat gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg
     4261 cgcggcggtc aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat
     4321 tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa
     4381 aaagctggtg tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca
     4441 agcactggcg gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata
     4501 cctgctcgcc catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt
     4561 cgctggctgg tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca
     4621 caatttgcac gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca
     4681 aagtacacat tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga
     4741 aaatccaata tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct
     4801 gtgtttgtca tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa
     4861 gtggttaaat tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat
     4921 ggaactgctt ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact
     4981 gccgctaatt gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg
     5041 cgtaacgcag acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg
     5101 tgtttctgtc accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga
     5161 aattgtgatt gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg
     5221 gctgctggct ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc
     5281 aggcgctaac cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc
     5341 gcaaggtttc gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc
     5401 gctgccggaa ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt
     5461 cgcgccagaa ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg
     5521 tcacgctacg ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt
     5581 tccgctggaa gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg
     5641 ttcatagtgc ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt
     5701 aatactcgtc ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac
     5761 aaaacagcga gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt
     5821 caatgctctg cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat
     5881 taagaatgcg aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag
     5941 agaagaacac cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca
     6001 gttccttcaa cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt
     6061 cgctgcccat gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga
     6121 tctccgccca gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat
     6181 acagcttaat ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct
     6241 cctgccgtgg ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt
     6301 cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg
     6361 cgctgacatc cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa
     6421 tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa
     6481 ggttgtagaa gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca
     6541 ttgtctctgc cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca
     6601 gcagactacc cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg
     6661 ccagcaggcc ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga
     6721 taatgcctgc agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga
     6781 taatggcgaa catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa
     6841 agatcacctg aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca
     6901 gcgccagcaa aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact
     6961 gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata
     7021 agtcgaaggt gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga
     7081 ccagagccgg tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg
     7141 tggcattact gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct
     7201 ttttttcgac gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc
     7261 cggttcgaac aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca
     7321 gcgttaacac gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc
     7381 aaaggaccgg aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg
     7441 gctaagatta ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc
     7501 actcattgag cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt
     7561 cataccccag attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc
     7621 gggcatagtc ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg
     7681 gatgactgaa ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga
     7741 gtttcatgcc agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat
     7801 gttccgggca ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca
     7861 gttcttcgtt gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct
     7921 catggagata ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt
     7981 tgttgtaatc atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc
     8041 cagtcacggc aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt
     8101 cagcgagaca gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga
     8161 cgttgatcac atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt
     8221 ttgccgggaa caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat
     8281 ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt
     8341 ccagaatgcg cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga
     8401 tgtaattacg cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca
     8461 ccggcggcgt gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg
     8521 tgaccggtgg tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct
     8581 tgcaggtacg agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac
     8641 caacggtgcg cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt
     8701 tcaccgccgt gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc
     8761 cctgacgctc catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc
     8821 ccgaaaggcc aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag
     8881 gcaccataac gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga
     8941 tatttttacc gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg
     9001 ttttaccctg ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca
     9061 tgacgcgcgc cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc
     9121 cgaccacgtt catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct
     9181 gctggcgggc ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt
     9241 attccagacg tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt
     9301 cgtccgcttt actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt
     9361 cacgacgatt aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt
     9421 aaatcgccag ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt
     9481 agaggaacac tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa
     9541 cgttagatac gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg
     9601 cgccgataaa gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga
     9661 tgttcgggtc gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt
     9721 acgcccgacg gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa
     9781 aggccatcac caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg
     9841 cgtcgacgcc ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca
     9901 gacctttttc aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat
     9961 ccggttcacc ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc
    10021 cttcatccat gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat
    10081 ccagcgcctc gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga
    10141 cgttggtgcg ttcacccacg ttcacaaaca ggctatcatc gccaatgttc agcggctcca
    10201 ggccagacaa acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta
    10261 ctgcacgact catcgctgca atatgttgcg gcatggtccc gcagcaacca ccgacgatat
    10321 tgagaaagcc cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat
    10381 cgtattcacc aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg
    10441 caatccgtga tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca
    10501 ggccaaaggt cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc
    10561 cggagagcgt gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa
    10621 cgcccagcgc ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt
    10681 cgaaaacggt ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt
    10741 cgcgataggc cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt
    10801 ccgaagaaat agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt
    10861 tctccggcgt gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa
    10921 agttgatttc cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt
    10981 tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg
    11041 cgatcacttc cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc
    11101 agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg
    11161 tgcccatacc gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca
    11221 ctttgctgct cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa
    11281 ccatactggc ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc
    11341 aacattaact catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa
    11401 taatttccat gatacagaaa aaggaggctg tcatggtcgc ccccattccc gcgaaacgcg
    11461 gcagaaaacc cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg
    11521 gcctgaaatt actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg
    11581 cgcaacaagc cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc
    11641 agggtttcgt gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta
    11701 tggtcggcag cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc
    11761 gcaatctaat ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc
    11821 acgaagcgat tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta
    11881 tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga
    11941 gcgaagaaca ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa
    12001 ctctggtgtc tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt
    12061 catttgacga tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg
    12121 agcaccgcga accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg
    12181 atcgcgtgac cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt
    12241 acggtggaat gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg
    12301 agtggcaata gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat
    12361 acacttcaca cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt
    12421 aacaaaatcg caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg
    12481 atttatgata tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa
    12541 attcaagaca tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct
    12601 ttctctgcaa atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg
    12661 ttttataaag aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa
    12721 agcttctcag agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc
    12781 attaaatttt ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa
    12841 aacgaaaaca cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaattgaa
    12901 attttacctc aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga
    12961 aaagaaaaag aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc
    13021 aaaaccgtat agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg
    13081 ttgattacaa accccatcaa gatgattact cttcagctga aaaatttctc actcacttca
    13141 acaaaaaatg caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg
    13201 ttgctgcctg cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa
    13261 atggcatcag tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc
    13321 taagaataaa gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta
    13381 atgtaactaa cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt
    13441 ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta
    13501 ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag
    13561 gaactgtgga tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa
    13621 ttggtcagaa tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac
    13681 atagaacaga aatgttgact gcctataata gtattaacct ccccggttta tatttggcta
    13741 taaattatgg caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat
    13801 atgaagggtt actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg
    13861 gtttttctgg attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc
    13921 tgaacgtctt acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat
    13981 tcctgagtgc caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg
    14041 atgccgacat gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt
    14101 tgactaaaga acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac
    14161 tatacctggc gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc
    14221 cctgtctggc ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta
    14281 aaagtcttgc gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta
    14341 ttaatactat ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa
    14401 tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat
    14461 acagcataaa caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg
    14521 cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa
    14581 atactctgcc tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct
    14641 taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc
    14701 ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat
    14761 ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt
    14821 agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca
    14881 atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag
    14941 acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg
    15001 cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg
    15061 tgacgggtga caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca
    15121 agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact
    15181 tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca
    15241 atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat
    15301 accggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt
    15361 cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac
    15421 atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc
    15481 agcgtaaaca ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta
    15541 cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag
    15601 ccgatagcca tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg
    15661 accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca
    15721 atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc
    15781 tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc
    15841 caggcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg
    15901 ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta
    15961 ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag
    16021 ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct
    16081 ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga
    16141 cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc
    16201 cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta
    16261 atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt
    16321 ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa
    16381 gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc
    16441 agtaattcca ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc
    16501 ggcgtaaaca ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca
    16561 agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc
    16621 tacagtcagc aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc
    16681 gcggtgactg aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta
    16741 cccacttcct gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc
    16801 tgctgcactt tctcaacata gtgcttcata ctttcgccct gcgcataagc gtttgccagg
    16861 tcgaacatgt taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag
    16921 cccatatccg acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc
    16981 cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata
    17041 gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg
    17101 tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta
    17161 cggaagaagc cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa
    17221 tctgaggtga tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg
    17281 cccgtcacgt cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc
    17341 actaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag
    17401 tgaactgccg cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca
    17461 ccgccaaaac cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga
    17521 tcgcccggct caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg
    17581 atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg
    17641 ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata
    17701 cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag
    17761 ccttttttcg actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc
    17821 gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga
    17881 gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta
    17941 cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc
    18001 ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg
    18061 cggcatcgtc aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt
    18121 tcatctcttc gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca
    18181 acgttttttg atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg
    18241 catcttccat caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt
    18301 actgcacagc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag
    18361 gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat
    18421 ttttacggga gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt
    18481 gagcgatcca tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt
    18541 tgttgagcac ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc
    18601 ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca
    18661 ggaatggttt atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt
    18721 tcaacgtttt gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat
    18781 ggtcgcgcag cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca
    18841 gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga
    18901 cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac
    18961 cgcagccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca
    19021 ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc
    19081 gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat
    19141 tggtatagct gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt
    19201 tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt
    19261 tcagtgcgtt gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt
    19321 ctaagtccgc aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg
    19381 aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca
    19441 gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt
    19501 cggcagtaag aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg
    19561 ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc
    19621 tgtgaacgaa ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa
    19681 tgcaaaaaca aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa
    19741 tatgaataaa atttattcac tttctgaggt gcgtttcgga aataaaaaag gcacccgaag
    19801 gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc
    19861 gttggattca tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag
    19921 ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg
    19981 ttgtgcggga aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa
    20041 tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc
    20101 ttatctttgc tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga
    20161 tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg
    20221 aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa
    20281 acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg
    20341 gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc
    20401 acctgtttga tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca
    20461 ccggttacaa tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag
    20521 ttgttcagat gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga
    20581 atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc
    20641 ttcttcggca tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca
    20701 cgagaagttg tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg
    20761 tccggcacac gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca
    20821 tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga
    20881 gaaagtttca aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg
    20941 ttattagtat tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc
    21001 gatctgtcga tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc
    21061 tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagc
    21121 cggttgggtc tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg
    21181 tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac
    21241 tgacaaccaa cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt
    21301 ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc
    21361 tggcgtatgt gggggataag ccgcccctgc cgtgcctgcc caagctggag aagacgcgcc
    21421 agcgtcgcac ccggcatttt tttcaccaac gcaaaaaggc catcctgacg
//
LOCUS       DAJTYI010000062        21239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYI010000062 DAJTYI010000000
VERSION     DAJTYI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(32..1012)
                     /locus_tag="OO564_002232"
     CDS             complement(32..1012)
                     /locus_tag="OO564_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6653344.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="OO564_002233"
     CDS             complement(1020..1730)
                     /locus_tag="OO564_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6653345.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OO564_002234"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OO564_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6653346.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="OO564_002235"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="OO564_002235"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6653347.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFAESTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="OO564_002236"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="OO564_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6653348.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="OO564_002237"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="OO564_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6653349.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /locus_tag="OO564_002238"
     CDS             8306..8416
                     /locus_tag="OO564_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6653350.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OO564_002239"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OO564_002239"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6653351.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OO564_002240"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OO564_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6653352.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="OO564_002241"
     CDS             11298..11930
                     /locus_tag="OO564_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6653353.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OO564_002242"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OO564_002242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6653354.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15462..16394
                     /locus_tag="OO564_002243"
     CDS             15462..16394
                     /locus_tag="OO564_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6653355.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="OO564_002244"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="OO564_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OO564_002245"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OO564_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6653356.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OO564_002246"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OO564_002246"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6653357.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="OO564_002247"
     CDS             complement(19726..19995)
                     /locus_tag="OO564_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6653358.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="OO564_002248"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="OO564_002248"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6653359.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="OO564_002249"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="OO564_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6653360.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5179 a         5345 c         5120 g         5595 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgag tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcactttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DAJTYI010000063        21045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYI010000063 DAJTYI010000000
VERSION     DAJTYI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..3096)
                     /locus_tag="OO564_002250"
                     /pseudo
     CDS             complement(1..3096)
                     /locus_tag="OO564_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(3103..3276)
                     /locus_tag="OO564_002251"
     CDS             complement(3103..3276)
                     /locus_tag="OO564_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653361.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3394..4587
                     /locus_tag="OO564_002252"
     CDS             3394..4587
                     /locus_tag="OO564_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6653362.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4785..6038)
                     /gene="glyA"
                     /locus_tag="OO564_002253"
     CDS             complement(4785..6038)
                     /gene="glyA"
                     /locus_tag="OO564_002253"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6653363.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6365..7555
                     /gene="hmpA"
                     /locus_tag="OO564_002254"
     CDS             6365..7555
                     /gene="hmpA"
                     /locus_tag="OO564_002254"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6653364.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7600..7938)
                     /gene="glnB"
                     /locus_tag="OO564_002255"
     CDS             complement(7600..7938)
                     /gene="glnB"
                     /locus_tag="OO564_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6653365.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7999..9333)
                     /gene="glrR"
                     /locus_tag="OO564_002256"
     CDS             complement(7999..9333)
                     /gene="glrR"
                     /locus_tag="OO564_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6653366.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9323..10036)
                     /gene="qseG"
                     /locus_tag="OO564_002257"
     CDS             complement(9323..10036)
                     /gene="qseG"
                     /locus_tag="OO564_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6653367.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10201..11628)
                     /gene="qseE"
                     /locus_tag="OO564_002258"
     CDS             complement(10201..11628)
                     /gene="qseE"
                     /locus_tag="OO564_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6653368.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12186..16073)
                     /gene="purL"
                     /locus_tag="OO564_002259"
                     /gene_synonym="purI"
     CDS             complement(12186..16073)
                     /gene="purL"
                     /locus_tag="OO564_002259"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6653369.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16331..17887
                     /gene="mltF"
                     /locus_tag="OO564_002260"
     CDS             16331..17887
                     /gene="mltF"
                     /locus_tag="OO564_002260"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6653370.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17884..18420)
                     /gene="tadA"
                     /locus_tag="OO564_002261"
     CDS             complement(17884..18420)
                     /gene="tadA"
                     /locus_tag="OO564_002261"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6653371.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18445..19080)
                     /gene="yfhb"
                     /locus_tag="OO564_002262"
     CDS             complement(18445..19080)
                     /gene="yfhb"
                     /locus_tag="OO564_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6653372.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19289..20137
                     /locus_tag="OO564_002263"
     CDS             19289..20137
                     /locus_tag="OO564_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6653373.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20476..21030)
                     /gene="pinE"
                     /locus_tag="OO564_002264"
                     /pseudo
     CDS             complement(20476..21030)
                     /gene="pinE"
                     /locus_tag="OO564_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5054 a         5686 c         5356 g         4949 t
ORIGIN      
        1 aacgtagtta aaaataaaag gcatcacagt ggtgaataat gccgctttcg gccaggccgg
       61 attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc cgagtcccgc
      121 ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta aatcgccata
      181 cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac tggttttcgc
      241 catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt gctgttcacc
      301 cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat cgttgtaata
      361 actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac gcatcgcttc
      421 agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt cgttatcagt
      481 ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg catgaagcag
      541 ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta ataactggtt
      601 gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga atttacgcgt
      661 ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat tgagcagttc
      721 tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac gcgccagtcg
      781 tttctccggt gtggctccac ttaacttatc cagcaaatcc cgttcgaaaa gcagacgtgc
      841 atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct cataatcgtg
      901 tttcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact ccggcgacat
      961 ctctacgcaa cgttgccata aggcaatggc tttgttgtag ctacgtttgt tgtagtagaa
     1021 gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg ccgccacttc
     1081 ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt ttgcgaccag
     1141 ttcgccacgt tcggctttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg
     1201 tgcgctggca gtccagtgcg ttcagcatct ctgccgccag agtgggcatt ccccagttaa
     1261 tcagttgccc ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact
     1321 gcgcgagggc tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca
     1381 gcggataatc acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
     1441 acaccagcag cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac
     1501 tttgttggca aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca
     1561 gaccataata gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt
     1621 cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc
     1681 cacactgcgg gtttttgttc agcgcatgtg cgcgtcctga ctggcatacg ccaccgcttg
     1741 cgggaaatct gcgcggttat attccagcat cgccagcgcc aggttacagc gataatccag
     1801 cgggtccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata
     1861 ttgctccaga tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc
     1921 cagtggcgct ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt cctgataact
     1981 cagtacaata ttgccgtcgg catcggagag ttcaatggtc agtctgtcag gattgatacc
     2041 gtgcaacacg ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc
     2101 gttgcatttg ccgatttcgc ggatcgccag acggtatccg ttcaacggag agatggcata
     2161 cagcccccac tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc
     2221 attttgcacc atgcacagag aatgataagg caggaaatac tgctcgaaac gcttctcttc
     2281 gtaagcatca agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc
     2341 gatatacggg ccgttattgt cggtcagact cttatcccac gcctggccaa attcactgtg
     2401 tccccaactc cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc
     2461 gccatcttca tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt cggccatata
     2521 tgaggttgga acaggcacat ttttatagcg agaaatgtcc actccggcgg agtagtccac
     2581 tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa
     2641 caccgcagtc acatccggtg ggaaaacgct ctgatgccct tcaccccctt tcactgccgg
     2701 gttggcccac cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat
     2761 ttccagtgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat
     2821 cggctccgtt tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa
     2881 atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc
     2941 ggagatccac ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac
     3001 aaaatcgcgc tgtttcactt tatcccatgc gcgatgcacc cgaccgccca gttccggcag
     3061 gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgctaggatt tcagggtttt
     3121 ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata
     3181 aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat
     3241 ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca
     3301 tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata
     3361 atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag
     3421 attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat
     3481 aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag
     3541 gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg aaagccagac gcgcgggcat
     3601 agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg
     3661 cccaccagta ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag
     3721 tatttacaga ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg
     3781 catcgccacc gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggg
     3841 caggttgatc cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca
     3901 gttgaggtga agtatctgct ggaagagaag ctgggcattc gggtgctggt cgataatgac
     3961 tgcgtgatgc tggcgcaggc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc
     4021 gtgatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc
     4081 ggcagtttgt atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc
     4141 tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa
     4201 caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg
     4261 acaaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggatcac cagctgggtt
     4321 gatcgctctg ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt
     4381 aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt
     4441 attcgccaga caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg
     4501 caaattggct tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat
     4561 gttgaggcgc agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga
     4621 agccttgatg atcataaacg aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg
     4681 agattgcaat atattgaatt tgcaagattt tgtaggcctg agaaggtgtt catgccgcat
     4741 ccaacatgaa caacgagcac attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg
     4801 taacgtgcgc agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg
     4861 atgctgtcca gcacgtcaca catccagcca gccagttctt tcgcttcagc ttctttaaag
     4921 ccgcgacggg taatcgccgg agtacctaca cgaataccgg aggtcacaaa cgggctcttc
     4981 ggatcgttcg gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct
     5041 gcttctttac cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg
     5101 ccgccggaaa ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt
     5161 ttcgcgacct gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc
     5221 gctttaccgg cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag
     5281 ttcagttttt tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga
     5341 cccgccaggg ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg
     5401 tagacgccag cagcaaccag gcccgcaacg tgcgccatat caacgaacag ataagcaccg
     5461 atgctgtcag cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaaa
     5521 ccaccgataa tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg
     5581 atatgaccgg tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta
     5641 accggagaac cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca
     5701 cctggttcca gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg
     5761 acgttagcgt agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg
     5821 atatcaacat actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg
     5881 ttggtcagct gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg
     5941 atcagttcga tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg
     6001 gcatcataat cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa
     6061 caataaaatt tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga
     6121 atcattgtgc gataacaggt cttgacaaag gaatttacgc aaacgattac cttaaggcta
     6181 cgcaaggctt tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg
     6241 atgcaaattt ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg
     6301 cagggctatt ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaggaaga
     6361 ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga
     6421 aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact
     6481 caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa
     6541 cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga
     6601 aaaaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg
     6661 tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc
     6721 gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta
     6781 taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc
     6841 taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc
     6901 agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc
     6961 gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat
     7021 cgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt
     7081 tggcgatgtc gtgaaactgg tcgctccagc aggtgatttc tttatggctg tcgcagatga
     7141 cacaccagtg acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga
     7201 cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg
     7261 cgaagttcac gcctttgctg atgaagttaa ggaactgggg cagtcactgc cgcgctttac
     7321 cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag
     7381 cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt
     7441 ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg
     7501 cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga
     7561 tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc
     7621 ttcaccggta cggatgcgaa tgacccgagc cacgtcaaag acgaagattt taccgtcacc
     7681 gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc
     7741 cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg
     7801 gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc
     7861 ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat
     7921 cgcatcaatc tttttcatgc tattccttga aaaggttgtc tgtcttttga tctgctaaac
     7981 gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag
     8041 cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc
     8101 tttggtgatt tgcagcagct tacgcaaata gttgagttca aaatgattac gtgcctcaac
     8161 aaaggttggc agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat
     8221 caccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt
     8281 acccggccag ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa
     8341 cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc
     8401 tgtgcgctcc gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc
     8461 ttcgcggaat tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg cagaaataat
     8521 ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa
     8581 cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag
     8641 cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc
     8701 gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt
     8761 aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat
     8821 ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag
     8881 ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca
     8941 gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc
     9001 atctttatcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc
     9061 atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg
     9121 gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa
     9181 atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac
     9241 actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc
     9301 gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg
     9361 cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg
     9421 agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct
     9481 gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga
     9541 gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc
     9601 agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg
     9661 cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt
     9721 taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag
     9781 gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg
     9841 atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat
     9901 aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc
     9961 aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt
    10021 gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca
    10081 gacagtctgg acatggacag cgcggagaaa ccggggcgta catatcgtac gtgaagattt
    10141 cgagcactac ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca
    10201 ttatttcgtg tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc
    10261 gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc
    10321 cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa
    10381 gatcatggcg cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac
    10441 ccgcgcacca tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac
    10501 cgcattggag taaagattat ccagtacgct catcagcagc attggctccg ccaagcaagc
    10561 tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc
    10621 agaaaccact gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc
    10681 cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcagctgct
    10741 gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc
    10801 agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa
    10861 atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag
    10921 aatacgttgc ccaaccgagc gtaactcgct cggtccacag aacgaaacgc tattgcccag
    10981 agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat
    11041 catccgcgtg aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca
    11101 accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac
    11161 cactgtgcgc gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc
    11221 ggcggcggca gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc
    11281 ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat
    11341 ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac
    11401 gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg
    11461 ccgggcatca ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag
    11521 gctttgccag gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag
    11581 cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc
    11641 gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa
    11701 ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga
    11761 tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt
    11821 ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt
    11881 atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt
    11941 gagagattta tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga
    12001 gacaacttaa gataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa
    12061 agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct cccagagttg
    12121 ggagcttatg atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg
    12181 atgacttacc ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg
    12241 ccccagtttt ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc
    12301 atcataatgg tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg
    12361 ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc
    12421 accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct
    12481 tcaccatgag agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac
    12541 gggctttggg ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc
    12601 acaaaacgtg gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg
    12661 caaccgttac atacccccag ggccagcgtt tgcggacgat ggaagaaggt tgcaaactca
    12721 tcgcgtacac ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca
    12781 tcaccgtagg agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt
    12841 ccagtcagca ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct
    12901 gccgccattt caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt
    12961 gcgccagtgg caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca
    13021 ttcaggcccg gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg
    13081 ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg
    13141 cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc
    13201 gcctgcccaa cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc
    13261 gcttcacggt cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc
    13321 gccaggcgat cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag
    13381 gccatttccg ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc
    13441 ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg
    13501 cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc
    13561 agcgcgttat tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc
    13621 tgcggcgtga tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac
    13681 agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata
    13741 gagtctttac ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct
    13801 ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg
    13861 gcggaaagtt taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct
    13921 tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg
    13981 ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta
    14041 gcgaccggca cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg
    14101 cggtcaccga tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt
    14161 ttcaccgcgt ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc
    14221 gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg
    14281 atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca
    14341 ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga
    14401 tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg
    14461 ctcatgcccg gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg
    14521 tcgctcacca gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac
    14581 agaattgggt tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc
    14641 tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca
    14701 gacgccatgg aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga
    14761 acgaccagct tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg
    14821 ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg
    14881 ctgttcactt tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg
    14941 ttgttaaacg ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc ggtaacaatg
    15001 cgctcaggct taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg
    15061 gatacggaga aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca
    15121 tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg
    15181 tggttgtgag tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg
    15241 cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta
    15301 tctttataag cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg
    15361 aacagcgatt tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg
    15421 cggcagtgct cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga
    15481 ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca
    15541 agacgcaggt tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg
    15601 accggagtcg gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc
    15661 gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc
    15721 gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg
    15781 cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg
    15841 gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc
    15901 aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg
    15961 acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt
    16021 ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc
    16081 tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct
    16141 gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt
    16201 gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt
    16261 tatgagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag
    16321 aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct
    16381 gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc
    16441 cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa
    16501 cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta
    16561 cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct
    16621 tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa
    16681 aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg
    16741 tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg
    16801 tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg
    16861 gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact
    16921 cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct
    16981 cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg
    17041 cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac
    17101 gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac
    17161 gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa
    17221 atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg
    17281 ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac
    17341 cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt
    17401 gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat
    17461 ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct
    17521 gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt
    17581 acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc
    17641 ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga
    17701 gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc
    17761 gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc
    17821 aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca
    17881 aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg
    17941 aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac
    18001 tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt
    18061 cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca
    18121 cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat
    18181 caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc
    18241 aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac
    18301 cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta
    18361 ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat
    18421 tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac
    18481 tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc
    18541 actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc
    18601 cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag
    18661 attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga
    18721 gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg
    18781 aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt
    18841 ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca
    18901 gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac
    18961 aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc
    19021 ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa
    19081 accatactcc tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga
    19141 tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc
    19201 atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg
    19261 tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta
    19321 ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac
    19381 ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt
    19441 cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag
    19501 tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg
    19561 tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta
    19621 cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc
    19681 tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc
    19741 ctggaagctg atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact
    19801 cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt
    19861 acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc
    19921 gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat
    19981 tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt
    20041 aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat
    20101 tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc
    20161 cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca
    20221 tcaattgtga tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa
    20281 tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa
    20341 cacaaagaac accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg
    20401 caaaaggcct gtgacataca acattaactt ttcagtcaac cgtagcacgt ccttgtatac
    20461 gccgtgtctg cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc
    20521 aacatcatag atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc
    20581 ctgcgcccat tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg
    20641 agcagcttcc agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag
    20701 ggcacccatc acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt
    20761 cagactacga aagttgatgc ctcgttcgcg caactcctcc accagcacga caagatgccg
    20821 catactgcgc cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt
    20881 cctgagcagt tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa
    20941 aatcagctcg catcctgcac agttcaacgc attacgttgt agatcggtat tctggtcatt
    21001 tgttgatacg cgtacatagc caataagcat ggtaaatccc cctgt
//
LOCUS       DAJTYI010000064        20970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYI010000064 DAJTYI010000000
VERSION     DAJTYI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..769)
                     /gene="fbp"
                     /locus_tag="OO564_002265"
     CDS             complement(<1..769)
                     /gene="fbp"
                     /locus_tag="OO564_002265"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6653374.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGI"
     gene            945..2318
                     /gene="mpl"
                     /locus_tag="OO564_002266"
     CDS             945..2318
                     /gene="mpl"
                     /locus_tag="OO564_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6653375.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2401..2952)
                     /locus_tag="OO564_002267"
     CDS             complement(2401..2952)
                     /locus_tag="OO564_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6653376.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3046..4398
                     /gene="pmbA"
                     /locus_tag="OO564_002268"
     CDS             3046..4398
                     /gene="pmbA"
                     /locus_tag="OO564_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6653377.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4581..4967
                     /gene="cybC"
                     /locus_tag="OO564_002269"
     CDS             4581..4967
                     /gene="cybC"
                     /locus_tag="OO564_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6653378.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5012..5476)
                     /gene="nrdG"
                     /locus_tag="OO564_002270"
     CDS             complement(5012..5476)
                     /gene="nrdG"
                     /locus_tag="OO564_002270"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6653379.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5634..7772)
                     /gene="nrdD"
                     /locus_tag="OO564_002271"
     CDS             complement(5634..7772)
                     /gene="nrdD"
                     /locus_tag="OO564_002271"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6653380.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8166..9821)
                     /gene="treC"
                     /locus_tag="OO564_002272"
     CDS             complement(8166..9821)
                     /gene="treC"
                     /locus_tag="OO564_002272"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6653381.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(9871..11292)
                     /gene="treB"
                     /locus_tag="OO564_002273"
                     /pseudo
     CDS             complement(9871..11292)
                     /gene="treB"
                     /locus_tag="OO564_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11411..12358)
                     /gene="treR"
                     /locus_tag="OO564_002274"
     CDS             complement(11411..12358)
                     /gene="treR"
                     /locus_tag="OO564_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6653382.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12543..12596
                     /gene="mgtL"
                     /locus_tag="OO564_002275"
     CDS             12543..12596
                     /gene="mgtL"
                     /locus_tag="OO564_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6653383.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12737..15433
                     /gene="mgtA"
                     /locus_tag="OO564_002276"
     CDS             12737..15433
                     /gene="mgtA"
                     /locus_tag="OO564_002276"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6653384.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15639..16025)
                     /gene="ridA"
                     /locus_tag="OO564_002277"
     CDS             complement(15639..16025)
                     /gene="ridA"
                     /locus_tag="OO564_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6653385.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16098..16559)
                     /gene="pyrI"
                     /locus_tag="OO564_002278"
     CDS             complement(16098..16559)
                     /gene="pyrI"
                     /locus_tag="OO564_002278"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6653386.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16572..17507)
                     /gene="pyrB"
                     /locus_tag="OO564_002279"
     CDS             complement(16572..17507)
                     /gene="pyrB"
                     /locus_tag="OO564_002279"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6653387.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17511..17645)
                     /gene="pyrL"
                     /locus_tag="OO564_002280"
     CDS             complement(17511..17645)
                     /gene="pyrL"
                     /locus_tag="OO564_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6653388.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(17926..18321)
                     /locus_tag="OO564_002281"
     CDS             complement(17926..18321)
                     /locus_tag="OO564_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6653389.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18453..19166)
                     /gene="bdcA"
                     /locus_tag="OO564_002282"
     CDS             complement(18453..19166)
                     /gene="bdcA"
                     /locus_tag="OO564_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6653390.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19237..19830
                     /locus_tag="OO564_002283"
     CDS             19237..19830
                     /locus_tag="OO564_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6653391.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            19975..20427
                     /gene="tabA"
                     /locus_tag="OO564_002284"
     CDS             19975..20427
                     /gene="tabA"
                     /locus_tag="OO564_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6653392.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20550..>20970
                     /locus_tag="OO564_002285"
     CDS             20550..>20970
                     /locus_tag="OO564_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6653393.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIF"
BASE COUNT         5034 a         5226 c         5526 g         5184 t
ORIGIN      
        1 aaataccgcc tttcagcagg ttacggtgga aatccgcaac cagtgaaccg atataacgcg
       61 aggtatacgg gcgattggtg gatttatctt cttcctggca gaatttaatg tacttcttca
      121 ccccgttcgg aaacttaatg tagtttcctt cgttgatgga gtaggttttg cctttctccg
      181 ggaagcgcat ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg taagtaaagg
      241 cgtgaacacc gcatccggtg gtgtaaacca gcatggtaga ggagccgtat accacgtaac
      301 ctgccgcaac ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc ggcgtgccaa
      361 caggcgtaac gcggcggtag atggagaaaa tggtaccgac agagacgtta acatcgatgt
      421 tggacgagcc atccaggggg tccatcagca ccacgtattt tgcgtgttca cagccttcaa
      481 agacgacaat ctcatcttct tcttcagagg caatccccgc aacgatatcg cgtgctttca
      541 gtgcggcttt cagtttttca ttagcgaaca agtcgagttt ttgctgaacc tcgccctgca
      601 cgttctcagc accgctggca cccaggatat caaccagtcc tgctttgttg atatcgcgat
      661 ggataatctt ggcgcccagt tttattgccg acagcaaagc agtgagctca ccggtagcat
      721 gagaaaactc gtgctgcttt tcgacaataa attcacctaa cgttttcata aaactttccc
      781 tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc aatgttgcgc
      841 tcaggtgaat cgcgccagca aattgcggat tatcctgaaa tgcgtttctc acttgcccga
      901 catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc attcatattt
      961 taggaatttg tggcacattt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg
     1021 aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag
     1081 gcattgagct gattcagggt tacgatgcca gccagctcga gccgcagccg gatctggtga
     1141 ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca
     1201 tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcacgac cgctgggtgc
     1261 tagccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc tggattctgg
     1321 aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac tttgaggttt
     1381 cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct
     1441 tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc
     1501 ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc
     1561 atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat gacatcaacc
     1621 tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt gagcagggtc
     1681 actggcaggc gaaaaagctg accaccgatg cttccgaatg ggaagttttg ctggatggcg
     1741 aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga
     1801 tggcgattgc agcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg
     1861 gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg
     1921 tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg gcgctgcgtg
     1981 gcaaagttgg tggtacggcg cgcattattg ctgtgctgga accgcgctcg aataccatga
     2041 aaatggggat ctgcaaagac gatctggcac cttcattagg tcgtgccgat gaagtcttcc
     2101 tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc
     2161 ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc
     2221 ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc
     2281 tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc ctgatatagc
     2341 attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca acaacgaaaa
     2401 ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg cggatttcgg
     2461 cggcttattc ccttctttct ctttcttcgc gttgcggatc agtgtacgca gttgctgacg
     2521 atcggcatcg ggccacagat tcaatacttc agcgatggcg tcatcacctt gatcgatcag
     2581 acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt gacggttttt
     2641 cagcttatcc agcgcctgac gaataggctc tacgtcgcgc tggcgcagca tcttaccgat
     2701 gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct caatagccgg
     2761 gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat caacaatttc
     2821 cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac tgacccagat
     2881 aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc agtcttcggg
     2941 ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt aagatgcgca
     3001 ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc acttgcaatg
     3061 aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt ttccactgcg
     3121 ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa gaccaccggc
     3181 attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag tgatggcgcg
     3241 ctggggatca ctgtttatca ccagaaccgt aaaggtagcg catcgtctac cgatttaagc
     3301 ccgcaggcca ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta tacctcgcca
     3361 gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc gaatctcgac
     3421 ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc ccgcgcagaa
     3481 caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag ctttaacagc
     3541 cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta ctgctcaacg
     3601 cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga gcgtgattac
     3661 gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt cggggccgac
     3721 tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat gaaagcgccg
     3781 gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg ggcgatagcg
     3841 ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa acaaattctg
     3901 ccggactggc tgaccattga agagcatccg catctgctga aagggctggc gtcgacgcca
     3961 ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg catcctgact
     4021 cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac cggacatgcg
     4081 ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca aatgctcaaa
     4141 gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag tgccattacc
     4201 ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat tcagtatccc
     4261 gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat tgtcaccgtc
     4321 ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt gccggagatg
     4381 aaaatcgccg ggcagtaaaa aactggctcg acctgccgcg ctataaacta aaaaattaca
     4441 caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag cgtactcgca
     4501 gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg atgaagtgta
     4561 aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca gtctcctcgt
     4621 tggtattcag ttctgcgtcg tttgccgctg atcttgaaga caatatggaa accctcaacg
     4681 acaatttaaa agtgatcgaa aaagcggata acgcggcgca agtcaaagac gcgttaacga
     4741 agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc gaagataaat
     4801 caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg gtcggtcaga
     4861 ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag gctgctgcag
     4921 agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc ctcatttccc
     4981 tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc acctgattgc
     5041 tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg ccgtcgacca
     5101 gcacgttaat caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg agtttatagc
     5161 ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgtttt accagtttca
     5221 gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa atcccctggc
     5281 gtttgatacg agtgtcattc agatctttaa tgatcttgtc ttccattgct ttggtaaatg
     5341 gctgaccaga atttacccgc catgtgcttt tgttatagca accggggcat tcatgaacac
     5401 acccggagac aaacagggtg cagcgagtgc cggggccgtt gacgatgtcg acaggatagt
     5461 actgatgata attcattatt tcgcctggct tatcagtccg cgtgtaggct gcatccggta
     5521 atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg ccaaatgtag
     5581 gccggataag gcgtttacgc cgcatccggc atttgcttag cgcagagtga agattaacct
     5641 atctgcccat tccccaaatg tttaacgcgg cgcttaactt cttcctgctt accagcgtta
     5701 aacggacgtg catccgggct acctaaatat ccgcacacgc ggcgagttac cgacacacgg
     5761 gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt tgctggtgca ctcgaactca
     5821 ccggtaaagc cacactcgta gcactcatca atcggtgtat tggtgccgta atacggtaca
     5881 tgctgatagc tgtaatccca gacatcttcc agcgctttca ggttgtgctg aatgtttgga
     5941 tactcgccgt agcaaatgaa accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg
     6001 atcttgtcgt acgggttcac cttcttctcc acatcaaggt ggaaactgtt ggtgtagtaa
     6061 cctttatcgg tcacgcccgg caccacgcca aactcagcag tatcgagacg gcagaagcgg
     6121 tcgcacaggt tttcactcgg cgtgctgtag agactgaaac cataacccgt ttcttctttc
     6181 cactgatcca ctgcctgacg cagacgttca acaatggcga tacctttcgc gcgaagcttc
     6241 tcgttgtcgt aaacatgctc gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg
     6301 taacccagcg aaatagacgc acgaccgttt ttgaagattt cagaaacatc atcgtcagca
     6361 ttcagacgta cgccacaagc accttccata tagaggatcg gggcgacgcg cgctttcacg
     6421 ccttcgagac gagcgatacg ggtcatcagt gccttacgtg ccagcaccag acgttcatcc
     6481 agcagcttcc agaaggtggc ttcatcgcct tttgcttcca gagcaatacg cggcaggttc
     6541 aggctgatca cgccgaggtt gttacgacca tcgtggatct gctcgccgtt ttcattttcc
     6601 cacacgccga ggaagctgcg gcagcccatc ggggttttaa acgaaccggt gactttcact
     6661 acctgatcgt agttcaggat atccggatac atgcgcttgc ttgcgcactc cagcgccagc
     6721 tgtttgatgt cgtagttcgg atcgcctttt ttatggttca ggccatcgcg aatcgcaaac
     6781 accagtttcg ggaacaccgc agttttacgg tttttaccga ggcctgcgat acggttacgc
     6841 aggatcgatt cctgaatcag gcgcgattcc cagctggtgc ccagaccaaa accaaaggtt
     6901 acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta cttcgtactc cagcgactgg
     6961 aaggcatcgt agcactcttt gatggtacga gagttagcgt agccttcggc gtccgggatg
     7021 ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg cggtaacaaa cggcgccagc
     7081 acttcatcga tacggttaat ggtggtgccg ccataaatat ggctggcgac ctgagcaata
     7141 atctgcgcag ttaccgcggt tgccgtagag atcgacttcg gcggttcaat ctcggcgttc
     7201 cccattttaa agccctgggt cagcatgcct ttcaggtcga tcaacatgca gttgaacatc
     7261 gggaagaacg gcgagtaatc gagatcgtga tagtgaatat cgccacgctc atgtgcctgc
     7321 accacgtcac gcggcagcag gtgctggcgt gcatagtgtt tagccacgat cccggccagc
     7381 aggtcgcgct gggttggaat caccttgctg tctttgttgg cgttttcgtt gagtaacgag
     7441 gcgttggtct gctcgaccag accacggatc tcctggttca ggcgaccgcg tttttcacgt
     7501 tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag ccagttgttt gtatggaccc
     7561 gacatcagct ggttttcaac tgcggtctgg atctcgttga tatccacctg gttgcgcccc
     7621 tgcatctgct cgttgacaac cgcggcaaca gtggcgcaat aatcggcatc atcgacttcc
     7681 gctgctttag ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa cggcactttg
     7741 cagccgtctc gtttcatcac atgcggtgtc atgatcgctc catattttta agaacaggtt
     7801 atccacagaa attgggaaag gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc
     7861 gcaatcgcca tccgcttatc cacaacgccg cacctttttc gtggggagct gtagtagcaa
     7921 ttatagtcga ttaatacaac atattgggtt gggacgcatt ttaaagtcta tatatagtgc
     7981 tttgcatcaa ggatgtttta actttttttg atgtagctca aagtaaaaag cagagcgtac
     8041 ggatgacggg cgctacagcg ttatgtaaat tttttaatga atttgctggt tgaaaaatca
     8101 acaaaaacaa catgctgaca gacaaaaccc cggaatgacc ggcattatcg gggcttaggg
     8161 aagatttact tctgtaacca ccagacagcc tcaaaaggcc gtaaattcat ggcacagggt
     8221 tgtggtgagg cttcttcgta gttatgcatc acaagctgcc agttgccgcg catttgccct
     8281 ggctgccagg gttggatctc acggctaagg ttggcaatga ccagcaaggt ttgccccttc
     8341 cattcacggc gatagcacca caatacaggg ctgtttggca gcagatcctg gtaattgccc
     8401 catgtcagga tggcttcttg cttacgcagt gcgattaact tttggtaggt gtaaaacaca
     8461 gaggagtcat cggccagcgc ggcttctacg ttgatttgtt gatagttatc gcctaggcca
     8521 atccacggtt cgccagccgt aaacccggca ttatcgccgt tgctccattg catgggcgtg
     8581 cggctgttgt cacgggattt actggcgagg attgccaata actcgtcggc atcacgacca
     8641 tcgttgcgca gctcggcaaa catattgagg ctctccacgt cgcgatagtc agtaatgcgc
     8701 gtgaaatgcg ggttggtcat gccaatctct tcgccctggt agatatacgg cgttccctgc
     8761 atgccatgca gcaccatcgc cagcattttt gccgcaggca cgcggtattc accttcatca
     8821 ccaaagcgag aaacaatgcg cggctgatcg tggttacacc agaacaaggc attccatgtc
     8881 acgttgtgca ttccttgttg ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt
     8941 ttagccaacg tccatttttc accaccggga taatcgacct tcaggtgatg aaaattaaag
     9001 gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg
     9061 gaggacattt cacctacggt cattaacccg cgtggcgtaa acacatcgcg gttcatctcg
     9121 tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc gacgcccgtc gccatccagg
     9181 tcttcaggga aacgcgggtc tttggagatc agattcacca catccaggcg caacccgtcg
     9241 accccacgat cggcccagaa ttcacagact tttttcagct ctgcgcgtac cgctggattc
     9301 tcccagttga gatccgcctg ttctggtgca aagagatgca aatagtactg ttcgctttcc
     9361 gtatgccagc gccacgcact accgccaaat tttgaacgcc agttgttcgg tggcgtttct
     9421 ggttcgccat cgcgccagat ataaaactgg cggtaagggc tttctttgtt cagcgcctcg
     9481 cgaaaccagg catgttgggt agaggtatgg ttaaacacca tatcgagaat gatacgaatc
     9541 ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt ccagcgtgcc gtaggtggga
     9601 tcaatcgccg tatagttcgc tacatcgtaa ccgttatcga cctggggaga gacataaaag
     9661 ggggttagcc agatggcatc aacgcccagt ttatgcagat agtccaggcg ttggataacg
     9721 ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg ataaatctgg
     9781 tagataacgc cgttttgcca ccagtgggga agattagtca ttacgttatt cctgcaaatg
     9841 cgagggggcg caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta
     9901 tttccgctga tagataaacg aggtgagtac aatcgggatg atgatggcga tagccattgc
     9961 cagcgcaaat acctgccagt agctcggttg aatcgagaga attcccggca ggccccctac
    10021 gccgatacca ttcgccataa cgccgttcag gccgcatagc aatcctgcca gaccagaacc
    10081 aatcatcgcg cacagcatcg ggaagcgata tttcaggttg atgccgtaca ttgcaggctc
    10141 agtgacccca agccaggcgg agatagcggc aggcacggag atctcgcgtt cattgtgctt
    10201 gcggctggaa atgataatgc ctatcacggc ggagccctga gcgatattcg acagcgcaat
    10261 cagcggccac actggcgtgc cacccatgct ttgaatcatc tgcaaatcaa tagcaagggt
    10321 ggtctggtgt acgccggtga tcaccagcgg cgcgtacagg aagccaaaca atgccgcacc
    10381 aatcggagca aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc catcgccaat
    10441 catgcgacca aacggaccaa tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca
    10501 gacgggcacc accaccagat agaggtaatc cggtacgatg cgtttaaggc gagtttcaat
    10561 aacgcccagc gccagcccgg ctaacagtgc cgggatcacc tgcgcctgat agcccacttt
    10621 ggcgatgctg aacatgccaa agtcccacac ttccggcagc tgctgcccga gcagataagc
    10681 gttcatcagc tgggaggaaa ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc
    10741 gcccattttt ttcaccgctg accagcaaat cccgaccggc aggtagaaga aaatcgcttc
    10801 accgatcaac cacagaaaat cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt
    10861 ctgaccgttg ctcatgggca aatcgccgat cacattgcga aaaccgagga tcaaaccgcc
    10921 gctaatcaac gcgggcagca acgggaagaa gatctccgcg aaatgagaga tcaactgctc
    10981 atgctatttc atattttgcc gggcggcttt ttttacctgc tctttatcaa cctgcgcctg
    11041 tccggttgac gctattagtg cttgatagta atcacccacg ttggtgccaa tcaccacctg
    11101 aaattgcccg gcattggtga aacagccttt caccatgggg agttgctcaa tttctttcgg
    11161 tctggcattg gccggttggt tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt
    11221 cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg tttggtttat
    11281 tttgctcatc ataaagcccc atggcagatg acatttttgg ttggctgcag aatatattgc
    11341 gcggaaaaat ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca atttatcgtt
    11401 caggaaacga tcaggacagg gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg
    11461 ttacctgtgc gatcaattgg caggccgcct ggcgtccagc ttcggcgtag ccaggatcta
    11521 cggtgacgat ctccggatga aggaatttca ttaacggcgt attacctacg ctcgccagtt
    11581 gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc gccaagagcc agcgtgtcgg
    11641 ttgcgcacag taacgcggta gtttcaggcg taatcacttt tgctacgttt tcatagcctt
    11701 gcttcatagc aagccctggc agagcggcaa cgggatgcag tttatgcgct ttgcagaacg
    11761 ccaggtaggc ttcgtgacgt cgcttcccgg tcgtcacgtc actatgcggc acgccgagat
    11821 aactgatatt gcgatgcccc tggtcataga gccgttgcat cagaatcttg attgcccctt
    11881 cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg
    11941 actgccagtg ggctaacatt tcttcggtta tgccggtaaa accgaacagc actacgccgt
    12001 cgatattacg ccgtttcagc acccccaaat gttcggcaac taattgtggg gaaaactgat
    12061 tttccatcat gattggatcg taaccttgtt catagaacgc tggcagcatg gtttgaacgg
    12121 cgagattttc tgacaacgaa tccagacggg taacaatgat ggcgaccact ttatcgcttt
    12181 gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc atgctgattc atcactgctt
    12241 caacacgctc gcgcgtgcgc tgactcacgc cgctttcgtt attcagcacc cgggaaaccg
    12301 tagatttccc cacgccgctt aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc
    12361 tgttgtccca taacgtgttg tttaattatt tgagcctaac tttacccgtg cattcagcaa
    12421 tgggtaaagt ctggtttatc gttggtttag ttgccagcag gtattatatc gccataaatg
    12481 ctacgaatat tattggattc tccttattat ttgcggcgct tttttcactt accggaggtt
    12541 atatggaacc tgatcccacg cctctccctc gacggagatt aaaacttttc cggtaagccc
    12601 gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt tttaatgtcc ctgctcagct
    12661 ttattacctt caggtaaggc ttcgccacgc ctgaagacat ttctgtactg tttcagacag
    12721 tgcggaggga ctccttatgt ttaaagaaat ttttacccgg ttcattcgcc atttaccttc
    12781 ccgtctggtt catcgcgatc cattgcctgg cgcgcagcag acagtgaata cggcggtccc
    12841 gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg cccgaagaag aattgtggaa
    12901 aacgttcgac acccatccgg aaggattaaa tcaggcggaa gtggaatctg cccgcgaaca
    12961 acatggtgat aataaattac ccgcccaaca accgtcgccg tggtgggtac atttatgggt
    13021 ctgctatcgc aaccccttta atattttgct caccattctt ggtgctattt cttacgccac
    13081 ggaagattta tttgccgcag gtgttatcgc gctaatggtc gctatttcta cgttgctgaa
    13141 ctttattcag gaagctcgtt ccaccaaagc ggcagatgcc ctgaaagcga tggtcagtaa
    13201 taccgcgacg gtactgcgag taattaacga caaaggcgaa aatggctggc tggagatccc
    13261 gatagaccag ctggtgcccg gcgatattat aaaactggcg gcgggggata tgatcccggc
    13321 agatttacgt atcttgcagg cgcgggatct gttcgtcgct caggcgtcgt taaccggtga
    13381 gtctctgccc gtggaaaaag ccgctactac tcgccagccg gagcacagca atccgctgga
    13441 gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac aagcaatggt
    13501 gattgctaca ggagccaaca cctggtttgg tcaactggcg gggcgtgtta gtgagcagga
    13561 aagcgagccg aatgcctttc agcaagggat cagccgcgtt agtatgctgc tgattcgctt
    13621 tatgctggtg atggctccgg tggtgttgct tatcaacggt tacaccaaag gcgactggtg
    13681 ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta acgccggaaa tgttgccgat
    13741 gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt
    13801 caaacatctg gatgccattc agaactttgg cgcaatggat attctgtgca ctgataaaac
    13861 cggcaccctg acgcaggaca aaattgtgct ggagaatcat accgatatct ccggtaaaac
    13921 cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat taccagaccg gacttaaaaa
    13981 tctgcttgat accgcggtgc tcgaaggtac ggatgaagag tcagcgcgtt cgctggccag
    14041 ccgttggcaa aaaattgatg agattccgtt tgatttcgag cgtcgtcgga tgtcggtggt
    14101 agtggcagaa aataccgaac accatcagct ggtttgcaaa ggtgcattgc aggaaatcct
    14161 caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgta ccgctcgatg acaccatgct
    14221 gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt
    14281 ggcgacgaaa tacctgccag cgcgtgaagg agattaccag cgggcggatg aatccgacct
    14341 gatcctcgaa ggatatattg ctttccttga tccgccaaaa gagacaaccg ctccggcact
    14401 gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt actggcgaca gtgagttagt
    14461 tgcagcgaaa gtgtgccatg aagtggggct ggatgcggga gaggtggtca ttggtagtga
    14521 tattgaaacg ctatctgacg atgaactggc aaatctcgca cagcgcacca cgctgtttgc
    14581 ccgcctgacg ccgatgcata aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt
    14641 ggttggcttt atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg
    14701 catttctgtg gacggcgcgg tagatattgc ccgtgaagcg gctgatatca tcctgctgga
    14761 aaaaagcctg atggtgctgg aagagggggt tattgaggga cgtcgcactt tcgccaacat
    14821 gctgaaatac atcaaattga cggcgagttc taacttcggt aatgtgttca gcgtgctggt
    14881 ggcgagtgct ttcttgccct tcctgccgat gttgccgtta cacttgctta ttcagaacct
    14941 gctgtacgat gtgtcacagg tggcgatccc gtttgataac gtcgacgacg agcaaattca
    15001 aaagccgcag cgttggaatc cggcggatct ggggcgcttt atggtcttct tcggaccgat
    15061 cagctcgatt ttcgatattc tgacgttttg cttgatgtgg tgggtattcc atgccaacac
    15121 gccggaaacg caaacgctgt tccagtcggg ctggtttgtg gtgggattac tgtcgcaaac
    15181 gctgattgtg catatgatcc gtacccgccg tgtgccgttt attcagagct gtgcatcgtg
    15241 gccgttaatg atcatgaccg tgatcgtgat gattgtcggg atcgcattgc cgttttcacc
    15301 gctggccagt tatctgcagc tgcaggcgct gccgttaagc tatttcccgt ggctggttgc
    15361 gattctggca gggtatatga cgttaaccca gttggtgaaa gggttctata gccgtcgtta
    15421 cggctggcag taaagaataa actgggcatg gtggcccgga ttcagattgc agacaaagtg
    15481 agctttgttc atgccggatg cggcgtaaac gcccaatccg gcctacaaat tcaataaatt
    15541 gcaggaatca cgtaggcctg ataagcgtag cgcatcgggc gattttgctt ttgtcatcag
    15601 acttaatccg ggcatgatag cccggatttc catcaagatt agcgacgaac agcgattgct
    15661 tcgatctcaa tcttcacgtc tttcggcaga cgggcaactt caacacaaga acgtgccggg
    15721 aaagtggcgt tgtgttcggt gaagaaggct tcgtaagtgg cgtttacggt tgcgaagtcg
    15781 ttcagatctt tcacgaacac agtggtttta acgatgtcgc ccactttcag gccagcggct
    15841 tcgacgattg cttttacgtt atccagcgac tgacgtgcct gtgcagcgac gtctgccggt
    15901 acttcaccag ttttcggatt taccgggatc tgaccggagg tgatgatcat attgcccaga
    15961 tcaacgccct gtacgtaagg accgatagct gccggtgcat tttccgtcgc gatagttttg
    16021 ctcatgattt ctcctttatt acagcggtaa aaagtctggc tcattatagg gagccagact
    16081 tttattacca accgcaatta attggccagc accacattat gggaaaactc tttttcacag
    16141 tatttgcatt taagcgcgat gtcattggcg cgttttcgca cggcaaagct ggatgaaacc
    16201 ggttcggcat ggctgataca gttgctgttc gggcagacca gcacattgtc gatgcgctcc
    16261 ggcagacttg ggcgcgattt acccaccact tcatagttgt cgatacggtt aaccgtggct
    16321 tgcggtgcat acaatgccag ttggtctact tgatcttcac tcaaaaaggt attttcgatt
    16381 ttgatcagat ctttgcggcc catctcgcca gaaggcaaat tcagaccaat ggtgatgcgc
    16441 tgatctgttt cggtcaactt gaacagactc aacagcttaa aaccgatctg ggcggggata
    16501 tggtcaatta ccgtgccgcg tttaatagct tcaacctgca atttattatc gtgtgtcatc
    16561 tctatttccc cttacagtac cagatcgcga ttcagaacca gtgccagtaa cgcctggcga
    16621 gcgaaaatcc cgttgcctgc ttgctggaag taccaggcgt gaggcgtttt atccacatcc
    16681 gtcgcaatct catcaacacg cggcagcgga tgcagcactt tcatattggc tttggcgttg
    16741 tggagatcgc tggcgcgaag aacaaactgc gctttcacgt tggcgtactc ggacgggtcc
    16801 agacgctctt tttgcacgcg ggtcatgtac aggatgtcta cttccgccat cacttcttca
    16861 atagagctgt gcagactcca ggcgatccct ttttcatcga gcatatccag aatgtattgc
    16921 ggcattgcca gcgcatccgg cgcgataaag taaaaacggt tgccgtcgaa cttcgctagc
    16981 gcctgggtca gggagtgaac ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg
    17041 agattgtcca gacgcccctg ggtttcctga atggtgaata agtccagcaa ggtttgcgtc
    17101 ggatgttggt tggagccatc accggcattc agtaccggta cattgccaga aaattcggtg
    17161 gccaggcgcg ccgcaccttc ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg
    17221 ataaccgaaa tggtatcggc cagcgtttcg ccttttttac ccagtgatgt attgccgctg
    17281 tcggagaagc ccaccacgct ggcacccagg cggtgcatgg aagtttcgaa agagaggcgg
    17341 gtacgggtag aggcttcgaa gaaacagctg gcaatgactt tgttcttcaa cagctctggt
    17401 tgcgggtttg ctttcagttt cgccgctgtc gccagcacca gattaaggtc atcgcgacta
    17461 aggtcgttta tggaaatgat atgtttctga tatagcggat tagccatctt ttatctcctg
    17521 acgcctaggc aaaaaaaagc ccctcgattg aggggctggg aatgggtgat caacgggaag
    17581 aaaaacggca ggccagcgtc ttttttcaga cgcggtaaga caaaatgtcg aacacactga
    17641 accatacatc ctcccggcaa attgtccggc attatactca tagccagaag cggtgcaagc
    17701 atttgatgca atattttgtc agcgcaaacg gtttatttga attaaaagtc aaaatatatg
    17761 catttttatt ttctatgatt ctggagggga agtgtcgttc ggtatcaata attgcagaca
    17821 tttcctgctt ttttcttttt cattttcacg caatcagaaa tgcattttat tccattcatc
    17881 cgacttatag ggcggagggt gaaaacgaac ggctaacact attgcttata gctcagggat
    17941 acgcgctatc actttaattt caaaatcaaa gcctgccagc caggtaacac ccaccgccgt
    18001 ccagtttgga taaggtgggg cgctaaatat ttcatttttc accgtcatga tgtcttcaaa
    18061 ttgtttttcc ggatcggtat gaaagctcgt aacatcaatg atatcgtcaa aagtgcatcc
    18121 cgcagctgcc agggtcgcat gcaaattatc aaaggccagt ctgacttgtt gctgaaaatc
    18181 gggttctggt gttccgtcct ctcgacttcc tacttgcccg gaaacaaaca gcaaatcgcc
    18241 ggaacgaata gccgcagaat aacgatgctc agcatacagt gaatgtcggc cagcagggaa
    18301 aacagcggtt ctttctacca tttggttatc ctcaagattt acgacatgaa cagaagattt
    18361 atatttaccg ggagccgctt ttagggggac gacgttagta aacaaaaccc agacatcatg
    18421 gataatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc atcaatggta
    18481 tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac cataccagcg
    18541 acctcttccg gttgcccatg tcttttgatg gccataaaac tatgcaacat atcgcgcatt
    18601 ggcccgttgg cgggattaac gtcggtatca attggtcctg gctggacgac gttgatggtg
    18661 atccctcgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgcag ggcagattta
    18721 ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac ggagccgatg
    18781 attaagatgc gcccgccttc gggcatctgc cgggcggctt caacagaggc atgataagga
    18841 gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc cagggcatcg
    18901 ccaaagacgc caatacctgc atttaccacc aggatatcca atgcgccgct cttacgaacg
    18961 acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc tccagtctct
    19021 tgtgccagac gttcagcggc atctttcgac cccgcatagg tgaatcgtac attggcccca
    19081 tcggtgacga aacgacgtac gatagcggca ccgattccac gactgccacc gaggatgaga
    19141 actgtcttac cggtaaaagc gcccataagg actccttgat ttattatgta acatgcatta
    19201 caaaactgtt ttaactttct gtcaacaggt tttgtaatgg ttactaaaaa acaatctcgc
    19261 gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc aaaagtgctt
    19321 tttcaccaaa aaggtttcga tgcagtaagt gtctctgaag ttactgatta tcttggtatt
    19381 aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag ccgtgtactc
    19441 aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcgtga tgatcgtccg
    19501 gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag ccaaaacggc
    19561 ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca agcgcgtgat
    19621 attgccattc aatattatca cgccgcagaa acgaccattt atgactatat cgccaggcgg
    19681 catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc agggctttct
    19741 gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc cctggcgggg
    19801 gaagcgataa aaactcttct caaggagtga tgctggcctt gatccgaaag gcgggaacgc
    19861 gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctggcg gaattgatca
    19921 tcttttcgta taacgataga aatgaaacat tgttttaatt aaggagtgga aaagatgatc
    19981 atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca ggcgattgag
    20041 catatcaaag cacacgttac ggcagaaacg ccaaagggca agcacgatat cgaaggcaat
    20101 cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg ccgtgcggag
    20161 taccatgccc gctatcttga cattcagatt gtgttaaaag gtcaggaagg catgaccttc
    20221 agcacgcaac ctgcaggcgc gccggatacc gactggttag ctgataaaga catcgcattt
    20281 ttgccggaag gcgttgatga gaaaacagtt atcctaaatg aaggtgattt tgttgtgttt
    20341 tatccggggg aagtgcataa accgctgtgc gcagtgggcg cgccagccca ggttcgcaaa
    20401 gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc caggtttacg
    20461 gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt gtaatgctta
    20521 gacgttctta ttaactcaag gagttcgtca tgagcaaaat atcagatttg aattattctc
    20581 aacacattac attagccgac aattttaaac aaaaaaatga agccttagat acctggtatg
    20641 tgggaatgaa tgattttgcc cgaattgccg gggggcagaa tagcagaagc aatattcttt
    20701 ctcctagagc atttttggag tttttggcaa agatatttac cctaggttat gtggatttta
    20761 gcaaacgctc caacgaagcg ggtagaaata tgatggctca tattgagttc tcatcttatt
    20821 ctaaagatac tgatggcaat gaaaaaatga agttttacat gaataatcct gaaggggagc
    20881 gagcggattt atctaaggtg aagatagaaa ttacacttgc ctcagcttct actaaaggga
    20941 ttcgtgaagg acatacagtc attatattta
//
LOCUS       DAJTYI010000065        20430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYI010000065 DAJTYI010000000
VERSION     DAJTYI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..491
                     /locus_tag="OO564_002286"
     CDS             <1..491
                     /locus_tag="OO564_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653394.1"
                     /translation="KAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRAL
                     NDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDA
                     NTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAEN
                     VE"
     gene            730..789
                     /locus_tag="OO564_002287"
     CDS             730..789
                     /locus_tag="OO564_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6653395.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1106..1165
                     /gene="ibsE"
                     /locus_tag="OO564_002288"
     CDS             1106..1165
                     /gene="ibsE"
                     /locus_tag="OO564_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6653396.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1285..2718)
                     /gene="hldE"
                     /locus_tag="OO564_002289"
     CDS             complement(1285..2718)
                     /gene="hldE"
                     /locus_tag="OO564_002289"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6653397.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2766..5606)
                     /gene="glnE"
                     /locus_tag="OO564_002290"
     CDS             complement(2766..5606)
                     /gene="glnE"
                     /locus_tag="OO564_002290"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6653398.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5629..6930)
                     /gene="ygiF"
                     /locus_tag="OO564_002291"
     CDS             complement(5629..6930)
                     /gene="ygiF"
                     /locus_tag="OO564_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6653399.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7172..7792
                     /locus_tag="OO564_002292"
     CDS             7172..7792
                     /locus_tag="OO564_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6653400.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7856..9094
                     /gene="cca"
                     /locus_tag="OO564_002293"
     CDS             7856..9094
                     /gene="cca"
                     /locus_tag="OO564_002293"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6653401.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9257..10078)
                     /gene="bacA"
                     /locus_tag="OO564_002294"
     CDS             complement(9257..10078)
                     /gene="bacA"
                     /locus_tag="OO564_002294"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6653402.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10169..10537)
                     /gene="folB"
                     /locus_tag="OO564_002295"
     CDS             complement(10169..10537)
                     /gene="folB"
                     /locus_tag="OO564_002295"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6653403.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10642..11259
                     /gene="plsY"
                     /locus_tag="OO564_002296"
     CDS             10642..11259
                     /gene="plsY"
                     /locus_tag="OO564_002296"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6653404.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11272..12203)
                     /gene="ttdR"
                     /locus_tag="OO564_002297"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11272..12203)
                     /gene="ttdR"
                     /locus_tag="OO564_002297"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12410..13253
                     /gene="ttdA"
                     /locus_tag="OO564_002298"
                     /pseudo
     CDS             12410..13253
                     /gene="ttdA"
                     /locus_tag="OO564_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13250..13855
                     /gene="ttdB"
                     /locus_tag="OO564_002299"
     CDS             13250..13855
                     /gene="ttdB"
                     /locus_tag="OO564_002299"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6653405.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13903..15300
                     /gene="ttdT"
                     /locus_tag="OO564_002300"
     CDS             13903..15300
                     /gene="ttdT"
                     /locus_tag="OO564_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6653406.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15343..16356)
                     /gene="tsaD"
                     /locus_tag="OO564_002301"
     CDS             complement(15343..16356)
                     /gene="tsaD"
                     /locus_tag="OO564_002301"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6653407.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            16594..16809
                     /gene="rpsU"
                     /locus_tag="OO564_002302"
     CDS             16594..16809
                     /gene="rpsU"
                     /locus_tag="OO564_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6653408.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16920..18665
                     /gene="dnaG"
                     /locus_tag="OO564_002303"
     CDS             16920..18665
                     /gene="dnaG"
                     /locus_tag="OO564_002303"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6653409.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18860..>20430
                     /gene="rpoD"
                     /locus_tag="OO564_002304"
     CDS             18860..>20430
                     /gene="rpoD"
                     /locus_tag="OO564_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6653410.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFI"
BASE COUNT         4902 a         5657 c         5228 g         4643 t
ORIGIN      
        1 ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa agaagccgca gagatgcagg
       61 cggcggctat cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag
      121 cacaacgtgc gctgaacgat gctatcaacg tactttctga tgaacaaacc agccttaaat
      181 tcaaactggc cttgttgcag gcgctacctg cggtaataga aaaatccgtt gagccgatga
      241 aatctattga cggtatcaag attattcagg tcgatggtct gaatcgtggc agcgctgcgg
      301 gtgatgcaaa cacgggtaat gtggggggcg gaaacctagc ggaacaagca ttatcagccg
      361 ctctctctta ccgcacacag gctccgctga ttgactcctt gctcaatgaa attggcgttt
      421 caggcggttc actggcggca ttgacttcac ccttaacctc aacaactccc gtcgccgaaa
      481 acgtagaata aattttcagc tatgaacgtc cctggcacaa aaagccgggg attttttata
      541 tctgcgttcc gctaaaaact gcaaatgatc aggtcgttgc aacgttttgc gtgaccgctc
      601 ggggaaggca aaactgcctc tgggaaagcg ttgcgcgagg tccggcgctc atcaacaatc
      661 ggggagtcag caaagggctg aaacgggaaa gcccctcccg aggaaggggc cttacagaag
      721 gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca
      781 gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt
      841 aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca catatccctg gcccaaaaag
      901 ccggggattt tttatatctg cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg
      961 ttttgcgtga ccgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg
     1021 gcgctcatca acaatcgggg agtcagcaag gggctgaaac gggaaagccc ctcccgagga
     1081 aggggcctta tagaaggaaa gggttatgat gaagttcgtc atcatactgg ttgtgttgtt
     1141 actgttaagt ttcccgactt actaacaact gatcagaggg gggagaaatc ctcccttacc
     1201 cttgttcctt tactttaggt tgaaaaaaca acagcgtcaa taggcctgcc tgctacgaag
     1261 cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgatgat
     1321 gttagtggtc gagcaaccgt cttcgaagtt gagcaccagc acttcgccgc cgttggccca
     1381 gacttcttta ctcccggcga tctcttctgg tttatagtcg ccgcccttca ccagtaaatc
     1441 tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc
     1501 gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttaa cggggcggga
     1561 atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca agcggtcacc
     1621 cagtttgcgg gcatttgcca gatagaagac gtgtccggcg tgcaggatgt caaagacgcc
     1681 attggtcatc accacttttt caccgcgttt acgcgctgcc gctacggcca gcttcagttc
     1741 ctcttcggtc atcacgccaa agccggtatc tgcgcgccca cgcactgcat tttccagctc
     1801 gatcggcgaa accgtggagg ttcccagttt gccgaccacc acgccagccg ccgcattggc
     1861 aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat
     1921 caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgggttg gcatatgcag
     1981 cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc
     2041 cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt
     2101 acatttaccg acaacagctt caaattccga gagattcggt gtcagcagcg tagcgccgcg
     2161 gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacgcccg ctttacgcgc
     2221 cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag aaagcaccag
     2281 cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc
     2341 ttcgaaacct tcttcaaaat ccagacggat cagctgttgg ttgcgggaaa gtacccgtaa
     2401 tttggtgatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc
     2461 cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga ccaggcgtgc
     2521 attagcaccg agagaagcga tattcatcgc cacgttagcc gcgccgcccg gacgttcttc
     2581 gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacgac tggtggggcc
     2641 gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc
     2701 tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc
     2761 atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagatc acgctctgcg
     2821 gtgaagcaat cctccgacac atggcccggc aattcctgta atgccagatg atgaagttca
     2881 tcgcgcagcg tcgtgtaagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg
     2941 ttttgcgcca gcagctccag aatacgcacg ttgtctgacc agcgcgttaa cttcggtttt
     3001 tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccgcct
     3061 tcatcggctt tgatatcaaa gcgattacga tgtttattgc cgagatgagc gcgcattttc
     3121 tcgcgcattt cccgcacttc cgtttgcaga gttttacctt ctcgcggcag tgtcataatc
     3181 tcacgacgca ccgcgtcaaa gtgcgcagtg agctgcggat cgccgtacac tacacgcgca
     3241 cgcaccagcg cctgatgttc ccacgtccag gcctcgtttt tctgataatc ggcaaatgct
     3301 gctgcggatg tcaccagcat tcccgccgcc ccggacggac gcagtcgagc atccacttca
     3361 tacaaaatgc cggaagaggt acgcgtactg aacagatgca taatgcgttg cgccagacgc
     3421 agataaaact gccgcccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa
     3481 tcatggagga agataaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg
     3541 ccgtagccga ccaccgcaaa accgcgccct tcacgttcgt tgaggtgatt tggcttaccg
     3601 tagcgggcca ccatttgacc ccacgcctgc tgaacgacgg catctatcat agcttctgcc
     3661 agccaggtta agtgatcgct cactttcatt accggtagcg taccggcgat atccgctgcc
     3721 gcaatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgttcttcg
     3781 tcatcttccg gcacgcgcag caaatactgg cgcagttcat cacggtaggc atcggtcgcc
     3841 gtcggctggt aaagggtatt tggatcgagc aattcatcca gcaataatgg ataacgcgct
     3901 agctggctgg caatcatcgg cgatgcggca cacagagaaa tcaaatgttt aagcgccgca
     3961 gggaattcac tgagcaattc taaataggtt gtgcgggtaa caatccccac cagcaaggcg
     4021 gtaatgcgcg acagcgtgac agcagcgtct tcacgcgcac agacatcact tagcagatgt
     4081 ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tagtacgctt atccagctct
     4141 ttgcggaaat cagcaatcag cgttaggacc tgtttgcgat catcctcgct aagatgcgcc
     4201 agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc
     4261 gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg
     4321 cgcacattgg tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc
     4381 atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtttg ggtttgttcg
     4441 tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatatgc cactcgcaat
     4501 tgttcagcat cgttttcaga aagcagatgc agcgcggcaa tggcgctgag cgttggcagt
     4561 aaagagcgcg actgcagcaa cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg
     4621 ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt
     4681 cgacgcactt cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa
     4741 tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg
     4801 ccttcgctat cgcccataat ccgcgctttg accatcgcgt aacgctccca gtcgcgtccc
     4861 tgctcctggt aataatcctc caacgcggca aagctcagca ccagcgggcc actttcgcca
     4921 aacggacgca gtcgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccagc
     4981 actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccggcgt
     5041 ccgccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc agaggagaaa
     5101 ttcagttccc cgccgcccag cttacccatg cctaagatca gcagcggttg cgcttcgccc
     5161 tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catacagcca gtcacgcgcc
     5221 gcaacaatca gcgtctccgc cagataactg agctgctgca atatgctctc ttcagtaacc
     5281 agtgccagcg tttgcgccca tgcgatgcgc accataatgc gccgccggaa tagccgcagc
     5341 tcgcgcatta acccggcttc gtcgctcaca ttactgagcg cctcctgcaa ccacacgctg
     5401 taatgttgcc attcgtcagc ctgtggcggt tggctttcca gttccgtcag ccactctgga
     5461 tgcgcaatca cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgca
     5521 ctaagtgatt cctcggctaa aagctctggc agccgctcaa caacggtctg ccagtactgc
     5581 tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc
     5641 gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcaat
     5701 gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag ttctccagcc aggcttgcgc
     5761 gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcatcaggcg
     5821 tggcaactgg tcacggtagc gatcgcctaa cggctggcag aaaacgcttt tcagttcagc
     5881 ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctaa ttttgccctg
     5941 cgctttggca tctaaaaacg gctgccatgc tttgcttacc agccattcgg tcaacgccag
     6001 cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg cagaagcgat
     6061 ggtcgcttcg cattgagtca gcaaatcacg taagtgagtg ctcgctttac gcggcacaat
     6121 gccaccgaac agcatcagtg tatgacggac taggctaatg gctgccagca cctgctcttt
     6181 cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacaccag
     6241 ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt
     6301 ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt
     6361 gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac
     6421 cgcgcgcgtg tcgccgctaa gcagctccag ttccagctcg cagataggct cagcaaattc
     6481 acccgctttc acttccccct ggtcgagagc gatttcaatt cggctgccat cgatctctac
     6541 tagccatttt tcgcgataaa aatcggtgct aaacagcggc tgcacgcggg aagcgagatc
     6601 ggcaggcaat tcgccgttcg gccagatttc cgtcggtaac tgcgccaggt cgagcgtcgg
     6661 ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc
     6721 aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg
     6781 cctacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca cggggtcatg
     6841 gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac
     6901 aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag
     6961 tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa
     7021 attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac
     7081 acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg
     7141 ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac
     7201 tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct atgtttctga
     7261 cgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt
     7321 taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt
     7381 gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag
     7441 cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac
     7501 caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag
     7561 cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg agctgattgt
     7621 cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat
     7681 catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt
     7741 actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact aaatcgcctt
     7801 cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa
     7861 gatttatctg gtcggtggtg ctgttcggga tgcattgtta gggctatcgg tcaaagacag
     7921 agattgggtg gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt
     7981 aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg
     8041 taccgaacgg aaatccggtt ccggttacac cggttttact tgctatgccg caccggatgt
     8101 cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga
     8161 taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg
     8221 ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg cgcgttttgc
     8281 tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga
     8341 gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacgga
     8401 aagcgccctc accacccgca atccccaggt gttcttccag gtactgcgcg attgcggcgc
     8461 acttcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggcac ctgccaagtg
     8521 gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct
     8581 gagtccgcag gttgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac
     8641 gccgccagaa ctttggccgc gtcatcatgg gcatggcccg gcgggtgtta agttagtgga
     8701 acaactatgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc
     8761 tgagtttcac gatctcatcc acaccttccc aatgctgaac ccaaaaacca tcgtcaaatt
     8821 atttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag
     8881 cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg
     8941 gttgcgcgaa ggctgggaag tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg
     9001 atttaaaggc gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag
     9061 ctggaaggaa caacgttgcc caaagcctga ctgagtataa aactaaacgt tagcacgaac
     9121 cgtcccctcg ccccttcggg gagagggtta gggtgagggg aacagaccag caccggtgcg
     9181 aacaatcacc cccaccccgg ccctctccct acagagcgag ggagaaaacc cggcctgctc
     9241 ccgaaccttt tctcaatcaa aagaacacga catacaccgc agccgccaca ataaagcgat
     9301 aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta
     9361 tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg
     9421 tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg
     9481 ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg
     9541 aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa
     9601 tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct
     9661 tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagcgcatac atcacattta
     9721 tcgggttaaa caatgactta atcgtgtcgt ggaataatag ccccagtacc accgccggaa
     9781 tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttcacct tcgtgctgca
     9841 acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta
     9901 ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcaa
     9961 accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg
    10021 tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca
    10081 ataaacccct aaattattaa aatgtaccgc ttgtccgaac tactgcagta tgaccaggtt
    10141 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    10201 caatgattac gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac
    10261 gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt
    10321 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    10381 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt
    10441 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    10501 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    10561 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    10621 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    10681 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    10741 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    10801 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcctgt ctggggcgcg
    10861 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    10921 cacatctggc ccgttttctt cggttttaaa ggaggaaaag gcgttgctac cgcttttgga
    10981 gcaatcgcac caattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    11041 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    11101 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    11161 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    11221 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    11281 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    11341 gcagacacga atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg
    11401 ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa cggcaacaca
    11461 tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcaa cactatctcg
    11521 ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg accgttcccc
    11581 aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc atgacgactt
    11641 aattcctgta atgattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat
    11701 attcttttat ttttcgttaa cagatgcgca atataataat caggaatttc gtcattaata
    11761 cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa
    11821 tgcacctgta actcaggata attgcgcata agttcggtaa tggctggcgc aatatggctg
    11881 cgcccaaaac caaaactgca accaatacga atcatcccct ccgggcgcgt tttgatttgc
    11941 gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa
    12001 cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag cgttgttgcc
    12061 agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc
    12121 gccgctgcag cactaaaact cccgctgtgg acgatttcta ccagtacctg caagtctttg
    12181 gctaaaggcc agctattcag catcgttaat tatccgcagt tgtgataagc gcagtgtatt
    12241 tcgcaaaaca ttgccattcc ctgctctggc gcaatattca ttcttatttt cgcgttgatt
    12301 cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt
    12361 agtttgtggc ggtgaatttt tccagacaaa tacaaaaact ggagttgcca tgatgagcga
    12421 aagtaataag caacaggcag tgaataagtt gacagagatt gtcgctaact ttaccgccat
    12481 gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga
    12541 aacgtcgtcg atggggaaaa ttatctacca tacgatgttc gacaacatgc aaaaagcgat
    12601 tgacctgaat cgtcctgcct gtcaggacac cggggagatt atgttcttcg ttaaagtcgg
    12661 ttcccgcttc ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc
    12721 aaccgtcaaa gcgccactac gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg
    12781 caaaaatacc ggtagcggcg taccgtgggt cacctgggac atcatccccg acaatgacga
    12841 tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg ctacctggcc gctcgaaagt
    12901 gttaatgccg tcagaaggct acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac
    12961 cctcgccgta aacgcctgtc caccggtact ggtgggcgtg ggcatcgcca cctcggtgga
    13021 aaccgccgcc gtactctcgc gtaaagccat tctgcgcccg attggcagcc gccatcccaa
    13081 tccaaaagcg gcagaactgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg
    13141 tccacaaggg ctgaccggca acagttcagt gatgggcgca tcgtcgcggc acgctgctgg
    13201 ttcatgccga tctcaccttt gaaaatctgt ctcacacccg gagcgcgtta tgaaaaagat
    13261 cctgacaacc ccgatcaaag ctgaagatct gcaagatatt cgcgtcggcg atgtgatcta
    13321 cctgaccggt acgctggtga cctgccgcga cgtttgtcac cgccgtttga tcgaactgaa
    13381 acgtccgatc ccttacgatc tcaacggcaa agcgattttc cacgctggcc ccatcgtgcg
    13441 caaaaacggc gacaaatggg agatggtctc cgtcggcccg acaaccagta tgcgtatgga
    13501 aagttttgaa cgtgaattta ttgagcagac cggcgtaaaa ctggtggttg gcaaaggtgg
    13561 tatggggccg ctgaccgaag aaggctgcca gaaattcaag gcgttacatg tgattttccc
    13621 ggcaggctgc gcggtgctgg cggcaaccca ggtagaagag attgaagaag tgcactggac
    13681 agaactcggg atgccggagt cactgtgggt ctgccgggtc aaagagttcg gcccgctgat
    13741 tgtctctatt gatacccacg gcaacaacct gatagccgaa aacaaaaagc tgttcgccga
    13801 acgccgcgat cccatcgtgg aagagatctg cgagcatgtc cattacatca aataaccctc
    13861 ccggagaggc tcacccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    13921 tggtggcgat atcttgcacc gctggcggtc atcgccatta ttgctctact tccggttccc
    13981 gcagggctgg agaaccatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
    14041 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc
    14101 atactctctc cctggctgct gttcagcccc gaacagctcg cccagccagg ctttaagttc
    14161 accgccaaat ccctctcctg ggcagtttct ggtttttcta attcggttat ctggctcatt
    14221 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg
    14281 ctgattctgg tgaaaaagat ggggcatcgc acgctgtttc tcggctatgc ggtgatgttc
    14341 tccgagctga tcctggcacc tgtaacaccg tccaactcgg cgcgtggtgc gggtattatc
    14401 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
    14461 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    14521 attttcctga cggcgatggc acctaacttg ctgttaattg gactgatgaa aagcgcatct
    14581 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag cattttactg
    14641 cttctgctgg ttccctggct ggcttacgtg ctatacccgc cggtactgaa gtctggtgat
    14701 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    14761 aaacggatgc tggggctgat ggtaggcgcg ctgatgctgc ttctgcgcat tatcagttgg
    14821 gacgacattg tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc
    14881 accctcgcga ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca
    14941 ggcagcttaa gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat
    15001 ctactgcgct actttttcgc cagcgccacg gcgtatacct ccgccctcgc accgatgatg
    15061 atcgccgccg cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc
    15121 gcggcaattg gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac
    15181 tacggtagtg gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg
    15241 atattcctcg tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa
    15301 gtgaggacgg tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc
    15361 cgccagcggc cagcgcggac gcacgctaac gccgagatcc gccgtcgcgc ctgctttaaa
    15421 ccgcaccatt ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc
    15481 gtagaacact tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg
    15541 gttagcactc acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg
    15601 cttacactta atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc
    15661 acgcgtctgg tcgtcggtgc tgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc
    15721 agaaaagctg aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc
    15781 ggcagtaccc tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag
    15841 cagcttcgcg gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag
    15901 ctcgtactga ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag
    15961 cgcgacaaac ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc
    16021 catatggtgt accgggattg ccggaacgtt ccaggcaaac gccagagagc gccccacggt
    16081 tgcgccaacc agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat
    16141 gtcttttgcc gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg
    16201 cacatgatcg cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac
    16261 ctgactatac aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt
    16321 ttcatcgcag gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta
    16381 ccgcgcagtg taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc
    16441 tcgtgtatac tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa
    16501 agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag
    16561 cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac
    16621 gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg
    16681 gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa
    16741 gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt
    16801 ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg
    16861 tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta
    16921 tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg
    16981 atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc
    17041 cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact
    17101 gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg
    17161 agttcgtcga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag
    17221 caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg
    17281 acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc
    17341 agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg
    17401 cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt
    17461 cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc
    17521 gcgaacgggt gatattcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc
    17581 gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata
    17641 aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc
    17701 tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg
    17761 ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga
    17821 ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag
    17881 cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc
    17941 cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc
    18001 ggatggagca ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg
    18061 atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc
    18121 aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca
    18181 tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc
    18241 gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa
    18301 atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg
    18361 gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgacctccg
    18421 ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga
    18481 tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc
    18541 atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc
    18601 atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa
    18661 agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag
    18721 aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg
    18781 gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa
    18841 taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac
    18901 ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga
    18961 tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca
    19021 ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga
    19081 agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac
    19141 gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga
    19201 aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaagttc aatgctccgt
    19261 tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga
    19321 agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct
    19381 ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga
    19441 agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc
    19501 ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac
    19561 catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga
    19621 agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg
    19681 cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca
    19741 gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac
    19801 ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc
    19861 tgaagaagtg catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac
    19921 catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg
    19981 tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata
    20041 caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa
    20101 agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg
    20161 gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt
    20221 gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat
    20281 gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat
    20341 ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga
    20401 tgaagattcg catctggggg atttcatcga
//
LOCUS       DAJTYI010000066        20293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYI010000066 DAJTYI010000000
VERSION     DAJTYI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            135..287
                     /gene="hokA"
                     /locus_tag="OO564_002305"
     CDS             135..287
                     /gene="hokA"
                     /locus_tag="OO564_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6653411.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(474..686)
                     /gene="cspA"
                     /locus_tag="OO564_002306"
     CDS             complement(474..686)
                     /gene="cspA"
                     /locus_tag="OO564_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6653412.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(967..1257)
                     /locus_tag="OO564_002307"
     CDS             complement(967..1257)
                     /locus_tag="OO564_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6653413.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1691..2401
                     /locus_tag="OO564_002308"
     CDS             1691..2401
                     /locus_tag="OO564_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6653414.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2451..3425)
                     /gene="ghrB"
                     /locus_tag="OO564_002309"
     CDS             complement(2451..3425)
                     /gene="ghrB"
                     /locus_tag="OO564_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6653415.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3529..4188)
                     /locus_tag="OO564_002310"
     CDS             complement(3529..4188)
                     /locus_tag="OO564_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6653416.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4341..6674
                     /gene="bisC"
                     /locus_tag="OO564_002311"
     CDS             4341..6674
                     /gene="bisC"
                     /locus_tag="OO564_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6653417.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6643..7083)
                     /locus_tag="OO564_002312"
     CDS             complement(6643..7083)
                     /locus_tag="OO564_002312"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6653418.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7080..7643)
                     /gene="tag"
                     /locus_tag="OO564_002313"
     CDS             complement(7080..7643)
                     /gene="tag"
                     /locus_tag="OO564_002313"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6653419.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7801..8499
                     /locus_tag="OO564_002314"
     CDS             7801..8499
                     /locus_tag="OO564_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6653420.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8728..9936
                     /locus_tag="OO564_002315"
     CDS             8728..9936
                     /locus_tag="OO564_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653421.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10260..11951
                     /gene="eptB"
                     /locus_tag="OO564_002316"
     CDS             10260..11951
                     /gene="eptB"
                     /locus_tag="OO564_002316"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6653422.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12043..12119
                     /locus_tag="OO564_002317"
     tRNA            12043..12119
                     /locus_tag="OO564_002317"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12077..12079,aa:Pro,seq:cgg)
     gene            12863..14470
                     /gene="dppA"
                     /locus_tag="OO564_002318"
     CDS             12863..14470
                     /gene="dppA"
                     /locus_tag="OO564_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6653423.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14778..15797
                     /gene="dppB"
                     /locus_tag="OO564_002319"
     CDS             14778..15797
                     /gene="dppB"
                     /locus_tag="OO564_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6653424.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15807..16709
                     /gene="dppC"
                     /locus_tag="OO564_002320"
     CDS             15807..16709
                     /gene="dppC"
                     /locus_tag="OO564_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6653425.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16720..17703
                     /gene="dppD"
                     /locus_tag="OO564_002321"
     CDS             16720..17703
                     /gene="dppD"
                     /locus_tag="OO564_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6653426.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17700..18704
                     /gene="dppF"
                     /locus_tag="OO564_002322"
     CDS             17700..18704
                     /gene="dppF"
                     /locus_tag="OO564_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6653427.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18734..20005)
                     /locus_tag="OO564_002323"
     CDS             complement(18734..20005)
                     /locus_tag="OO564_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6653428.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4856 a         5026 c         5328 g         5083 t
ORIGIN      
        1 tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg gtcagtacaa
       61 agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt ggaaatcaac
      121 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      181 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      241 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcggcgg
      301 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac atttattctt
      361 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      421 aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      481 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      541 tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg tacgaacaca
      601 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      661 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      721 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      781 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      841 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      901 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      961 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
     1021 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatagcgac
     1081 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcgtaaccg
     1141 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1201 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1261 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1321 ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt cgattcagcc
     1381 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1441 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1501 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1561 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1621 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1681 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1741 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1801 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1861 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1921 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1981 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     2041 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2101 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2161 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2221 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2281 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2341 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2401 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc ttagtccgcg
     2461 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2521 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2581 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2641 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2701 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2761 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2821 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2881 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2941 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     3001 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3061 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3121 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3181 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3241 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3301 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3361 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3421 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3481 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3541 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3601 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3661 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3721 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3781 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc tgctgctgtc
     3841 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3901 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3961 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     4021 cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4081 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4141 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     4201 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
     4261 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4321 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4381 cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4441 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4501 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
     4561 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
     4621 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4681 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4741 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt gcggcacagg
     4801 cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc agttggccgc
     4861 tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc aatacgctga
     4921 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     4981 gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     5041 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg
     5101 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat tgcaccacag
     5161 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg aaaaacgccg
     5221 aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     5281 tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     5341 agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg
     5401 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cggcgttctg
     5461 cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc
     5521 ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cacaacggta
     5581 tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc aactttactc
     5641 atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     5701 ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct gcgactacct
     5761 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     5821 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
     5881 tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc
     5941 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     6001 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     6061 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg catccgttaa
     6121 aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac ggttacccgg
     6181 attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
     6241 aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag ctgaattaca
     6301 gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt catcctgacg
     6361 atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac tcccgtgggc
     6421 agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt tgcattcacg
     6481 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga
     6541 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6601 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     6661 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6721 gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat aaacctccag
     6781 catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa taccgcgtct
     6841 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
     6901 accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa gataggcatc
     6961 ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
     7021 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
     7081 catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac cagcccacat
     7141 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca ttttttcagt
     7201 gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
     7261 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa cggttcgccg
     7321 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
     7381 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgctgcg
     7441 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
     7501 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
     7561 ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag cgggccctga
     7621 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7681 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7741 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7801 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
     7861 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     7921 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
     7981 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     8041 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     8101 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     8161 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
     8221 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     8281 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8341 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8401 aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac taataatagc
     8461 gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca caattgaaac
     8521 cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa taaatttggt
     8581 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
     8641 tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc agagattttt
     8701 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
     8761 ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta tacctggagc
     8821 ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt cgctttctct
     8881 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     8941 cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt aggcttcttc
     9001 ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
     9061 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
     9121 cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
     9181 aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt tgtgatttgg
     9241 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
     9301 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
     9361 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
     9421 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
     9481 gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct ggtgctgggc
     9541 attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt gatttcgctg
     9601 gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt
     9661 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact ggtcagtgag
     9721 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
     9781 ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt gaccttctac
     9841 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg tcagccagag
     9901 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag ctactgggct
     9961 tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact cgtaaaattt
    10021 gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
    10081 gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc gctttgctgt
    10141 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10201 ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10261 tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca atctatattg
    10321 gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg cacgatttta
    10381 ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10441 ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg
    10501 tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg gtcattggtt
    10561 atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    10621 tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt atctggaata
    10681 accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt attcgcagcc
    10741 tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    10801 agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag
    10861 cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg
    10921 tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt acataccagg
    10981 ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc acgcgttggg
    11041 accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc caggagaaaa
    11101 atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca ctgcgttgca
    11161 tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa gaacagaaca
    11221 ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg cagagcgaaa
    11281 tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag attggtgcgg
    11341 agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11401 cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggttcgcatt
    11461 ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg
    11521 ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg gtgacctatg
    11581 tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    11641 acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    11701 aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca gataaatatc
    11761 tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag
    11821 tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    11881 cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca aaagtggcag
    11941 cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    12001 attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct
    12061 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12121 attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg aggatgagaa
    12181 cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc agcgtcgcat
    12241 caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc gaccaacaca
    12301 ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac ggatttgtga
    12361 cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact tatggctggc
    12421 gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag atatccacct
    12481 tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt gctaaatatt
    12541 aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa atagctgttt
    12601 gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt
    12661 attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt tatggaggat
    12721 ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg ttgacccgac
    12781 cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt aaaacaacaa
    12841 acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga tgctgaagct
    12901 tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta
    12961 ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg gcaccaccta
    13021 tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg gcaccaccga
    13081 agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa cctatacctt
    13141 ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga cgcgcgaaat
    13201 gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa acccgtacca
    13261 taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg agctgatcag
    13321 cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac cggaagcgcc
    13381 gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat atgctgacgc
    13441 gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa ccggtccgtt
    13501 ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg atggctactg
    13561 gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg cttccgtgcg
    13621 ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc cggcagacat
    13681 cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc tgaacgtcgg
    13741 ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc gccaggcact
    13801 gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg cgggtgtatc
    13861 agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc aggattacac
    13921 ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa aaggtttctc
    13981 catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc gccgcatggc
    14041 ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg tcacctacga
    14101 atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa tgatgggctg
    14161 gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct gcgccgcctc
    14221 tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc tgattcaacc
    14281 ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag cgcaggtggt
    14341 gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg aatcggtacg
    14401 caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg aaaacgtctc
    14461 tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc tgatgtgctc
    14521 cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt tgtaggccgg
    14581 ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca atctgtatac
    14641 ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca ttctctgtcc
    14701 gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc tgcgggtcac
    14761 tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc gtcatcccca
    14821 cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg ggcgatccgg
    14881 tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag ctgctggctg
    14941 aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg ggcgtaatgc
    15001 acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag ttcgtgccgc
    15061 gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg gcagtcggta
    15121 ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac actgcagttg
    15181 gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg ctgatcatgc
    15241 tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc
    15301 tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc tggggtgaag
    15361 acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg ctgggtacta
    15421 ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg ctgggcgagg
    15481 attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt atcgtccatg
    15541 cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg ggaacattgc
    15601 tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg cgctggttga
    15661 ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg ctggtggcga
    15721 cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg aacccgcgta
    15781 ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa gtgattagcg
    15841 caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc aacaaaggcg
    15901 cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt gccaactgga
    15961 ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg ccagcctggc
    16021 aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt gatgtgctgt
    16081 cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg gtggtgttgt
    16141 cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc ctggttgata
    16201 acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg ttggcactgg
    16261 tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg accttcgttg
    16321 ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg aatcgcgatt
    16381 acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt attaacatct
    16441 tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct aacgccattc
    16501 tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg cctgagtggg
    16561 gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc
    16621 ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac ggtctgcgtg
    16681 acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt aaatgtagat
    16741 aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga ccgcatcagc
    16801 tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc cggtaagtcg
    16861 gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat ggcagaaaaa
    16921 ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg caacctggtg
    16981 ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc atgctacacc
    17041 gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa aagtacccgc
    17101 cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc ttcgcgtctg
    17161 gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat cgccatggcg
    17221 attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct ggacgtgacc
    17281 attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa catggcgctg
    17341 gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat cattgtgatg
    17401 tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc gccgcgtcac
    17461 ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg
    17521 gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg cctgcttaac
    17581 ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct gaatatgctc
    17641 gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag gcccacacta
    17701 tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac ctgaaaaaac
    17761 attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg ctggacggcg
    17821 tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta
    17881 aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc gagctgtatt
    17941 atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa
    18001 tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa gttgggcaaa
    18061 ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt cgggaaaaag
    18121 ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc tatccgcaca
    18181 tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg ctcgacccgg
    18241 atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt gcgcaggtgc
    18301 tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt atctcccacg
    18361 acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta ggccgctgcg
    18421 tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac acccaggcgc
    18481 tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc aaactcaccg
    18541 gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc cgctgtcgtc
    18601 ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga ggtcaactgg
    18661 tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca aaaccggggc
    18721 catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc aaaatgccgt
    18781 aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg actttataga
    18841 tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc aactgaaaga
    18901 acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg ataaaagcgc
    18961 agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt cggcttaaca
    19021 gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca aagaatgcgg
    19081 tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt ccagggatca
    19141 cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg gcttcttcgt
    19201 ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat
    19261 aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct gcttcccgtt
    19321 tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa aagaagcaaa
    19381 atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag gcggttatat
    19441 tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc cctactttga
    19501 ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac gcaatcgcta
    19561 tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg gcatcggtta
    19621 aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag tagataaaaa
    19681 tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag ttcttcccca
    19741 ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa agggttttta
    19801 aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg gtaataaata
    19861 cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca ccaatcgcca
    19921 tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt
    19981 gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa
    20041 atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa
    20101 aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac aaaattgcgc
    20161 atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca aataaagaaa
    20221 ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt aaaaagcaat
    20281 cgtaaaatca gcg
//
LOCUS       DAJTYI010000067        20135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYI010000067 DAJTYI010000000
VERSION     DAJTYI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..4283)
                     /gene="mukB"
                     /locus_tag="OO564_002324"
     CDS             complement(<1..4283)
                     /gene="mukB"
                     /locus_tag="OO564_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6653429.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQ"
     gene            complement(4283..4987)
                     /gene="mukE"
                     /locus_tag="OO564_002325"
     CDS             complement(4283..4987)
                     /gene="mukE"
                     /locus_tag="OO564_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6653430.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4968..6290)
                     /gene="mukF"
                     /locus_tag="OO564_002326"
     CDS             complement(4968..6290)
                     /gene="mukF"
                     /locus_tag="OO564_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6653431.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6287..7072)
                     /gene="cmoM"
                     /locus_tag="OO564_002327"
     CDS             complement(6287..7072)
                     /gene="cmoM"
                     /locus_tag="OO564_002327"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6653432.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7208..7987
                     /gene="elyC"
                     /locus_tag="OO564_002328"
     CDS             7208..7987
                     /gene="elyC"
                     /locus_tag="OO564_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6653433.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7964..8857)
                     /locus_tag="OO564_002329"
     CDS             complement(7964..8857)
                     /locus_tag="OO564_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6653434.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9011..9757)
                     /gene="kdsB"
                     /locus_tag="OO564_002330"
     CDS             complement(9011..9757)
                     /gene="kdsB"
                     /locus_tag="OO564_002330"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6653435.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9754..9936)
                     /gene="ycaR"
                     /locus_tag="OO564_002331"
     CDS             complement(9754..9936)
                     /gene="ycaR"
                     /locus_tag="OO564_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6653436.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9988..11220)
                     /locus_tag="OO564_002332"
     CDS             complement(9988..11220)
                     /locus_tag="OO564_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6653437.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11257..12243)
                     /gene="lpxK"
                     /locus_tag="OO564_002333"
     CDS             complement(11257..12243)
                     /gene="lpxK"
                     /locus_tag="OO564_002333"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6653438.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12240..13988)
                     /gene="msbA"
                     /locus_tag="OO564_002334"
     CDS             complement(12240..13988)
                     /gene="msbA"
                     /locus_tag="OO564_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6653439.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14025..16244)
                     /locus_tag="OO564_002335"
                     /pseudo
     CDS             complement(14025..16244)
                     /locus_tag="OO564_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16451..16735)
                     /gene="ihfB"
                     /locus_tag="OO564_002336"
     CDS             complement(16451..16735)
                     /gene="ihfB"
                     /locus_tag="OO564_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6653440.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16895..18568)
                     /gene="rpsA"
                     /locus_tag="OO564_002337"
     CDS             complement(16895..18568)
                     /gene="rpsA"
                     /locus_tag="OO564_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6653441.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18679..19362)
                     /gene="cmk"
                     /locus_tag="OO564_002338"
     CDS             complement(18679..19362)
                     /gene="cmk"
                     /locus_tag="OO564_002338"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6653442.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19535..>20135)
                     /gene="ycaL"
                     /locus_tag="OO564_002339"
     CDS             complement(19535..>20135)
                     /gene="ycaL"
                     /locus_tag="OO564_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6653443.1"
                     /translation="GCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYM
                     TSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAA
                     RDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLV
                     GSFEKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4824 a         5600 c         4897 g         4814 t
ORIGIN      
        1 atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc cagtcgcgct
       61 gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag gcggcgagat
      121 tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc aatcgcctca
      181 ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg gttaacctca
      241 acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt ctgcggcgtg
      301 cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa cgcttccgag
      361 aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc agagagcaca
      421 tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga gacgcacgct gttcacctga
      481 ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg ctcgcgctga
      541 atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt ctgttcacgg
      601 gaggttaatt cttcggtcag acggctaagt tcaatctcca tctgttcgat agcttccacc
      661 ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg ataaaccgcc
      721 acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg cagcacgtcg
      781 cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ctaacgcctt atccgacatg
      841 gaacgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg gcgctcaacg
      901 ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt cactacctgc
      961 tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag gttgtccatc
     1021 tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg gttattgctc
     1081 agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc gctatcagca
     1141 cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt tttggtgtcg
     1201 taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc tgcgtgaccg
     1261 cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgact gttccagacg ttcacgcagt
     1321 ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagaata gctaaagtgc
     1381 gcacgacgct gcaccacttc cgtcagggca aacgcttgct gacgggcatc gcgctgcatc
     1441 tgctgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc gctctgcaat
     1501 accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac aaaacgcgcg
     1561 gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc caggctgtca
     1621 tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc ttctttcgcc
     1681 tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa cgcccgctcc
     1741 agttcgactc gacggctgtt cagttgacgg atttctgctt ccgggtcaga ctcaaacgcc
     1801 accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg ggttttctgt
     1861 acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc atggaggctt
     1921 tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa acgtgaataa
     1981 cgccactgtc gatcggcgat tttcaccacc accgcttttt ccagctcatc aacgctgaac
     2041 acgctatcat cgaatgactg cggatcccct tcgatcaaat agagatcttc cgggcaatcg
     2101 gtcaagcctt ccaggtgttc ggttacctgt gacagatctg gcaccacgat ggcgtggcgt
     2161 gacgggccat acagagccga gaagtacggc gcatcttcca ggctaacgtc gtcataaatt
     2221 tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg atcttcagag
     2281 ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg cgcgcctact
     2341 tcatcgcgtt caacaatcgc ctcgcgctca cgctccagca actgttgcag atattcagtg
     2401 acgtcctggc tggaggagaa ctcttcgccg cactgttcac tcaactggtt gagactgttt
     2461 tgcgctgcca gccaaaccgg tgcacgcagc atcaaactct gaatgcgaga ctgcagctgt
     2521 tcctgttcct gccgcagtgc catccgctct tcacgggcgt tagatacgct atcggaaaga
     2581 gaggcaatgc gtgcttccag ctcctgatgc agagcttcca gttcatcaat atcaaaattc
     2641 ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg ctcacgcaga
     2701 cgctgttcca gttcgcttaa gcgcatccgc aacggctgaa cctgctctgc caggtgacgc
     2761 tgatcaaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt acgcgccagc
     2821 gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg cgcggtttgc
     2881 gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag ctctttcgcc
     2941 tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag atggcacagt
     3001 tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg cgtttgctgg
     3061 acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac ttccagttcg
     3121 gcagcttccg cgcgagcctc attctcttcc tggcgttcga tggcttctgc caccacttca
     3181 ttttgctctt ccagacggat ctgcagctca tcgagatcca cttcgtagcg ttcgatcttc
     3241 tcctgctgac gcaatgcggt ttgcaccagg ttcaggtgat cgctggccgc ctgataatcc
     3301 gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat atcgacgtgt
     3361 ttgtactgct cagccgccag ttgctgacgc gaagtatgta gctcgcgacg aaactccagg
     3421 gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc cgccacgtag
     3481 ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt gacacgaatc
     3541 gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa cgctttacgc
     3601 acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc actggagatc
     3661 ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga tgcggagcgc
     3721 agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt aatggagttg
     3781 aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc gttaagcggc
     3841 agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg ctgcaccgac
     3901 atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg tccggcaacc
     3961 tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt gtcgagcatc
     4021 gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga accgctggtg
     4081 gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat cagcgccgta
     4141 acgaacgccg ccatcgtggt ggatttaccc gccccgttac cgccggaaag cgtcgtgacc
     4201 agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag cgtcagtgag
     4261 cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga ctctcttcgg
     4321 tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca cgaatcaggc
     4381 gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag cggaacaccg
     4441 attcggtaat gcggaacttg ctgctgtcgt ggcccataaa ccacaccatg cctaaacgac
     4501 gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca acgtctgaac
     4561 cggttgaacg gttgttcacc agtttcagca gtttcgcttc atcggccagg gtgagcagtt
     4621 cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc gggctgagat
     4681 agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg gaacgcggga
     4741 taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga ataagctcaa
     4801 cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg aatgcatgat
     4861 tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc agcgccggaa
     4921 ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca atatttgtcg
     4981 atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt cgctggcagt
     5041 ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac aatacgcgca
     5101 acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac cagaccaaga
     5161 tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat cgccgccagc
     5221 tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc cccagtcact
     5281 tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc ggcattggca
     5341 taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa ccgctgagca
     5401 aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac gtggcggtcg
     5461 tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc gagtttgctc
     5521 tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat cgtcgcatcc
     5581 tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt atcctgcaat
     5641 tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat cgccgcccgc
     5701 cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg ttcgtccatc
     5761 agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt cagtggcgca
     5821 tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc tgctgcgcgt
     5881 ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga aaactcgcgc
     5941 tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta aattgcgttc
     6001 ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat gtcgttgatc
     6061 gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc actcacatgg
     6121 cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc gccgttcagc
     6181 gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga gaagtcattt
     6241 tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat actttatcct
     6301 tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc tagggtaata tacggttcct
     6361 gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc tgctggtgct
     6421 tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt tgccaaccag
     6481 cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc gaaagcgtcc
     6541 gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg accatgttat
     6601 gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cactctgcct ggacgcaaca
     6661 ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc accgcatgga
     6721 acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg gcgcaatgta
     6781 taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg cggtcgatca
     6841 tctgcgcaga aagatcgcat aaaataacct gatgcccgcg ctcggccatt ttgattgcgg
     6901 tctgcccttc tccgccgcca gcatccagca cacgcagttt ttgcgggccc atttccgcca
     6961 gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg gtggtgccgt
     7021 aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc attgctttct
     7081 ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc cacggcgaga
     7141 atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa aaaaggaacc
     7201 agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc cacttccgct
     7261 gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt ttcaaaaaac
     7321 cgggaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt tgcaaccggt
     7381 agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata attcgcaaaa
     7441 agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt gggcaccgag
     7501 ctctaattta atcaataaca gtctgccacg cctgaatgag ggtatccgtt tatggcgcga
     7561 aaatccgggt tcaaaactga tcttcacggg aggcgtagca aaaaccaata cggtgagtac
     7621 agcggaagta ggtaccagag ttgcgcaatc gctgggcgta ccgcgcgagc aaattatcac
     7681 cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg cgattggtga
     7741 cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga tcttttttca
     7801 gcaggaaggg ttaaatccgc taccagcccc ggcgaatcag ttggccatcg actcgccgct
     7861 taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg atcgtgttgg
     7921 ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg agccacggca
     7981 agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta ttaaccagtt
     8041 gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac tccgccactg
     8101 gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc attagtcgga
     8161 acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc gccagtaact
     8221 gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga atcaacacac
     8281 aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga aacaggatcc
     8341 gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc catagcactt
     8401 caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg accgcgccga
     8461 cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt tggtctttga
     8521 gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca cgcatccgct
     8581 ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca taaatagtcg
     8641 gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt tgtcgggctt
     8701 ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg ctgtcataca
     8761 aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg agtttttcgc
     8821 ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc ataaaagcta
     8881 ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca attgttgaaa
     8941 agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc tgaagtgtcg
     9001 tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg gtatccacac
     9061 ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac cacagaacac
     9121 gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg acgtaacgac
     9181 ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta tcgccaacgg
     9241 tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcacgt gagaagtaca
     9301 gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac gcttcttccg
     9361 cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg aggttatcag
     9421 caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca ttaacgatca
     9481 ccgtgtcgtc gctgaatgcg catttttcga caacttccac cagacgttct gttcctgact
     9541 gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg cgggcaacat
     9601 cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg cgttcaagaa
     9661 catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc agacgcgtgg
     9721 acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat cagcagtcag
     9781 cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga aagccaggtt
     9841 atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc cgttgcaaac
     9901 cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg gatcgggtta
     9961 tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc agccagtgcg
    10021 gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg cctgctgcca
    10081 gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg ttggtttaat
    10141 gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat gcattttggc
    10201 atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat agccatactg
    10261 gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa gctgctctgc
    10321 gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt gcgttgcagt
    10381 gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca gccaaagatt
    10441 gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg cttcgcgcca
    10501 cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt cgcggaatat
    10561 tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt gcgaaacgag
    10621 atcgcgctca tcatcccagt caggcatgac tcggtgggtt aaatcataaa cgcgctggaa
    10681 gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca gatgccgttt
    10741 atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa aatcggctga
    10801 acgtaccggc cctttatcat gaatatgctg aattaactgt gcaatttccg cctcatgttc
    10861 ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca tgcggtggcg
    10921 aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt attccattaa
    10981 ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca gacgactgaa
    11041 aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca gcaaggacat
    11101 gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt tgtttaacag
    11161 gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg gcagcgacat
    11221 tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag ccagcgaggt
    11281 tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta caggcaaata
    11341 ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag tcattaccag
    11401 cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact gatgatcggc
    11461 cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa aaaagcgcgg
    11521 cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt cacaccgcgt
    11581 accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt caccgctgcg
    11641 agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg ctcgctcacg
    11701 cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat caataacgac
    11761 aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta cgatgatctg
    11821 cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg gagaaaccgc
    11881 aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct gtgctgtggt
    11941 ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc cccgcgatac
    12001 gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa cgaccggggt
    12061 ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg gggcacgcca
    12121 ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca gaccatacaa
    12181 ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga ttttttcgat
    12241 cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc aagcaaatcg
    12301 ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc gtctgccttt
    12361 tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg caactcatcc
    12421 agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc ttcatccaga
    12481 atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg acgctgaccg
    12541 ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc catcttattg
    12601 atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg gctgtactgt
    12661 tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg gacattctgc
    12721 gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag atcgtgacca
    12781 tccatcagga tttcaccttc atcaatatcg taaaaacgcg tgatcaggct ggcaatggtt
    12841 gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg aattttcagg
    12901 ttgatgttac gcaatgcggg tacgtcacgt cccggataag taaaggtgac attgcggaat
    12961 tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc ctgctcactg
    13021 tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg ggcgttaacg
    13081 ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac aacggtaatc
    13141 gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag aacaaacgcc
    13201 agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc tgaaaccatt
    13261 ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt ttccacttcc
    13321 tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc actggtggtc
    13381 acttgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt cgatactacg
    13441 cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag ttgccaactg
    13501 taatagaaca tcatgatgaa caggccgatg atcgacgcac cttcacgcac aacagtaatc
    13561 agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg tgacaacagc
    13621 gtacccgttg actgtttgtc aaagaatgaa actggcattc ccatcatgtg accaaacagg
    13681 cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta gctggagaca
    13741 tagctggtga taccacgtaa aatcatcagt ccgatcacca ccagcggcat ccacaccagc
    13801 acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag cgataacatg
    13861 aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag acccgctttg
    13921 aaaggcgcaa tggttggcca cagtcggcgg aatgtctgcc acgtagagag atctttgtcg
    13981 ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc gttatccact
    14041 ggcacgccaa accactgata ataccaacga ggtaaaattt gctctctgag gctgctaatc
    14101 cgccagcctt gtgctgaaaa attgaccgtc acttgaccct gatgtggagt atcaagccat
    14161 tgatatcctt tctgttgata gcgatgctta actttgttag agggcaatcg ccatgcgtta
    14221 tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa tggcaacgat
    14281 gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgcctgcac ttgctgccag
    14341 taacggctta gcatcttttg ttcagctggg gcttcaatat caccggttag aagaatgcta
    14401 ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcccttatc gttgctacct
    14461 tgtaaaggcc agtgcgcgct gaaacgcaat ccttgccact gccacgcttc gccacgcaca
    14521 cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg ctatatatgc
    14581 aatattgaat ccagccctcc ccggtgatcc agatgttcat ggctcagaat aacgccttcc
    14641 ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact atccccttca
    14701 ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat caccattgcc
    14761 agcccttgcc cgacatcaag catgtacagc tgccactcgt caggtcgagg tttttgccac
    14821 agcggccagc acatcagcaa gcctccagcc acacacatta ctggcaacgt tcgccaggcg
    14881 tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg ccaacattca
    14941 acaatgttga tccacccttc cggcaaactc tttaacccct gttccaggat taacggcccg
    15001 ctaaaatgca caaccatcgc ggcgaggatc aacggaaccg tgataaatgt caccaaggga
    15061 attgcaaata gatttgcaat aaacgaggtc agactaatgc catgaaatat gacgatttgc
    15121 atgggcataa gcaggagtgt gattcccagt tgcagatgga tgagggaaac aactgcacgc
    15181 aataccggcg gcagttgcca ctcaggacag ggaaaccact gataccaaaa aatcagtgcc
    15241 gcgaccgcag cggcagagag ccataaactt tgcgagagaa tggcaacagg atccatcagc
    15301 aaaattgccg ccagacaaca tatccataca tcccagccac tccactgtcg cccacttaac
    15361 ttaagcattc cccacgtagc aagcgccacc acggtacgca atgcaggagg ttgcattccc
    15421 gtcaaccagg cataaaaagc agcacagcag attccgccaa ttaatggtat ttgccagtgg
    15481 atccagcgcc caggcagaaa aatttgtcca ctgcgaatga gtccggcagc cagcaacgcc
    15541 gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt atcgcgcatg
    15601 atatttttga tttctttggg gacggataac cgttccccca tacctaaacc aagaataacc
    15661 gcattccacg gatagggttg cagcgttgtt tgtagtgacg ccagatactg tgcacgcagg
    15721 ctacaattcg gttcaatgac acttgcctgc agaaaacggc cggtgagcgg ctgatgctgg
    15781 gcaatggcat aacgctggct atcaaagccg ccatcattaa gttggccatg aactgcacga
    15841 actttgagtt tcattgacca ttgttgtcca gcacaaaccg cttgcggaag ttattcgcca
    15901 tacagcacaa ggcctggcgc agggaatata cgtcgacctt gtagataagt aatttgaccg
    15961 taatgagtgg tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg cgtcgcgcca
    16021 gttaaggttt ctcctgccca caaaatttgc tttgctgata atatgcccca aacgaaaaac
    16081 agcaacgtca gcgcgatata acggacggtt ttaacaggga taaatgccag tacgcaggcg
    16141 aagagagtca gaaacgcaag ggttaatgtc ctaggcaatt ggggcaaaat cagcaacgga
    16201 aaaattccgc aaattatgca tacgccgact gtcgttattt tcatgccccc ctccctgttt
    16261 atggaaagtg tgcaactttg ttgagcggtt gtctggtggg aattttgcgt tccccgaggc
    16321 acattcagaa gaatttaacg aaagggtgaa aactgctcaa aactctgcga ggcagcttcc
    16381 agttcctgat tagagaattg aaaaaagcac ccgacaggtg cttttctcgc gttcaagttt
    16441 gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag ttctttacca ggtttaaagt
    16501 gaggaacgta ttttccttcc agttctactt tatcgccagt cttcggatta cgtccggtac
    16561 gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata cgctcgccct
    16621 gcgcaagagt cgaggccata tgctccagca tctcttttac tgcatcttca accgtcttgg
    16681 cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa ttctgacttg gtcatgattc
    16741 ctccggttcc ttaaaggcaa attaagcggc tgctgttgct gcattgatta agggcggccg
    16801 tagccgccct tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt aaactcaaca
    16861 aacttcggaa taaaaatccc gaagagtcag agaattactc gcctttagct gctttgaaag
    16921 cttcagccat tgcgttgttg gagaagtttg catcttcctg tttgttaaca gttgcgattg
    16981 catctttctc gtcagcttcg tctttcgcac gaacagacag gctgattgcg cggtttttac
    17041 gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac gctcagaacc agggtagcgt
    17101 cttcaacgcg gtcacgggat gcttcagaag cacgcaggta accttcaacg ccgtcagcca
    17161 gttctacggt tgcgccttta gcgtcaactg cagttacttt accggttacg atagcgcctt
    17221 tcttgttcag agcaacccag ttgttgaacg gatcttctgc gagctgttta acgcccaggg
    17281 agatacgttc acgttctgcg tcaacctgca gaacaactgc agcgatttcg tcgccttttt
    17341 tgtattcacg aactgcttct tcgcctgcaa cgttccagga gatgtcagac aggtgaacca
    17401 ggccgtcgat gccgccgtcc aggccgatga agataccgaa gtcagtgata gacttgattt
    17461 taccttcaac acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac gggttagctt
    17521 tgcactgttt cagccctagg gagatacgac gacgttcttc gtcgatatcc agaaccataa
    17581 cttccactac atcgccaacg ttaacaactt tggacgggtg gatgtttttg ttggtccaat
    17641 ccatttcgga aacgtgtacc agaccttcaa cgccttcttc gatttcaacg aagcagccgt
    17701 agtcagtcag gttggtcacg cgaccagtca gtttggtacc ttccggataa cgtttagcga
    17761 tagctaccca cggatcttcg cccagctgtt tcaggcccag ggatacacgg gtacgttcgc
    17821 ggtcgaactt cagcacttta acagtgattt cgtcgcccac gttgacgatt tcgctcggat
    17881 gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag gccgtcaact ccgcccagat
    17941 caacgaatgc accgtagtca gtgaggttct taacgatacc tttaacttcc atgccttcct
    18001 gcaggttttc cagcagctga tcgcgctctg cgctgttttc ggattcgata acggcacgac
    18061 gagaaacaac aacgttgttg cgcttctgat ccagcttgat tactttaaat tcaagctctt
    18121 tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa ccaggcagga
    18181 acgcacgaat accgttcagc tcaacagtga agccgccctt aactttgccg ttgataacac
    18241 cggtaacagt ttcagcatct tcgtaagctt tttccagcgt gatccaggct tcgtgacgtt
    18301 tagctttctc acgggacagc agagtttcac cgaagccgtc ttctactgcg tccagagcaa
    18361 cgtcaacttc gtcacctacc tggatttcca gctcgccctg ggcgtttttg aactgctcag
    18421 ccgggatggc ggactcagat ttcagaccag cgtcaaccag tactacgtct ttgtcgatag
    18481 caacaacaac gccacgaacg atagaacccg ggcgggtttc gatttctttt aaggactctt
    18541 caaagagttg agcaaaagat tcagtcatgt ttaatcttca ggtttatatt taacgtccac
    18601 ctggctccat gccggatggg gttgtttaac atacccgctg tcattccatt gcaacggggg
    18661 tactgcaaat tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt gtagcgcttt
    18721 ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac actaaagcat cggctgccgg
    18781 aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg cgttctttga tctcggccaa
    18841 aaggcgctcg aagttaacac taaagccctt ctcctgcaac tgtagcatgc ggcgatgcgc
    18901 acgttcttcc gaggaggcgt caaggaaaat tttcactggt gcatcaggga ataccaccgt
    18961 tcccatgtcg cggccatcgg caatcagacc tggtaattcg cgaaacgcgc gttggcgacg
    19021 caataatgct tcacgaacgc gtgggaatgc cgcgacttgt gaagccgcat tcgccacttc
    19081 ctgagtacga atttcgccgc tgacatcttc cccttcgagg atcacttcca gattgccatt
    19141 ggtcgacaca aaacgtacat ccagatggga tgccagcggt accagcgcat cttccgacgc
    19201 aacatcaaca tggtgatgta atgccgccaa tgccagtacg cgataaattg cacccgagtc
    19261 cagcagatgc cattgcaacg cttccgccat agccttacac aaggtgcctt tccctgcacc
    19321 gcttgggcca tcaatggtaa taaccggggc aattgccgtc atctttatct ccttaaaaca
    19381 ggcgtaccgt taacataacc gcgcgcatta tacgcgccga cgccacaaat cgttaacgtt
    19441 gtgtgcaaaa accagttttg tctgtattca gatggggatt tattgcgcaa ttatagcggg
    19501 ctggcagaag accagcccga aagatgacaa tgatttactt accagaggcg atacgatcac
    19561 ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc aaacatggac tgggtgcgac
    19621 cgccatccag gctagccagt ttttcaaagc tgccaaccag cccctgggtg ctgatgccac
    19681 gtttcttcaa cagatcaaaa gagaaatcat ctgcttcgga ctctttatca cgggagtatt
    19741 tagcattaat agcgccttct gcgatatcac ccaattgtga gcgggaaagc tgggaagcca
    19801 cgccgctggt agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa gctttcattt
    19861 cagccagcga gtgacccaac gcgacgtggc ccagttcatg gcccagaaca ccttcaattt
    19921 cgttgtcatt catcatgtcc atcaggccac tgtagacacg aacacagccg ttcgccatcg
    19981 cccatgcgtt gacgtcgctg gtcatataaa ccttatagtt gaccggcgtg ccattaatat
    20041 tgttacccaa tgctttggcg atttttgcca gacgtttacc gtacttgctg gatttacttg
    20101 cgacttgatt gccgctgtcc atttcggcac agccc
//
LOCUS       DAJTYI010000068        19838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYI010000068 DAJTYI010000000
VERSION     DAJTYI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     misc_feature    160..285
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            161..208
                     /gene="pheL"
                     /locus_tag="OO564_002340"
     CDS             161..208
                     /gene="pheL"
                     /locus_tag="OO564_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6653444.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            307..1467
                     /gene="pheA"
                     /locus_tag="OO564_002341"
     CDS             307..1467
                     /gene="pheA"
                     /locus_tag="OO564_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6653445.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1510..2631)
                     /gene="tyrA"
                     /locus_tag="OO564_002342"
     CDS             complement(1510..2631)
                     /gene="tyrA"
                     /locus_tag="OO564_002342"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6653446.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2642..3712)
                     /gene="aroF"
                     /locus_tag="OO564_002343"
     CDS             complement(2642..3712)
                     /gene="aroF"
                     /locus_tag="OO564_002343"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6653447.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            3921..4286
                     /locus_tag="OO564_002344"
     CDS             3921..4286
                     /locus_tag="OO564_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6653448.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4436..4954
                     /locus_tag="OO564_002345"
     CDS             4436..4954
                     /locus_tag="OO564_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6653449.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            4944..6169
                     /gene="dgcN"
                     /locus_tag="OO564_002346"
                     /pseudo
     CDS             4944..6169
                     /gene="dgcN"
                     /locus_tag="OO564_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6185..6667
                     /locus_tag="OO564_002347"
     CDS             6185..6667
                     /locus_tag="OO564_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6653450.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(6744..7091)
                     /gene="rplS"
                     /locus_tag="OO564_002348"
     CDS             complement(6744..7091)
                     /gene="rplS"
                     /locus_tag="OO564_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6653451.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7133..7900)
                     /gene="trmD"
                     /locus_tag="OO564_002349"
     CDS             complement(7133..7900)
                     /gene="trmD"
                     /locus_tag="OO564_002349"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6653452.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(7931..8479)
                     /gene="rimM"
                     /locus_tag="OO564_002350"
     CDS             complement(7931..8479)
                     /gene="rimM"
                     /locus_tag="OO564_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6653453.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8498..8746)
                     /gene="rpsP"
                     /locus_tag="OO564_002351"
     CDS             complement(8498..8746)
                     /gene="rpsP"
                     /locus_tag="OO564_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6653454.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(8883..10244)
                     /gene="ffh"
                     /locus_tag="OO564_002352"
     CDS             complement(8883..10244)
                     /gene="ffh"
                     /locus_tag="OO564_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6653455.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10411..11202
                     /locus_tag="OO564_002353"
     CDS             10411..11202
                     /locus_tag="OO564_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6653456.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11223..12509
                     /locus_tag="OO564_002354"
     CDS             11223..12509
                     /locus_tag="OO564_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6653457.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12564..13157)
                     /gene="grpE"
                     /locus_tag="OO564_002355"
     CDS             complement(12564..13157)
                     /gene="grpE"
                     /locus_tag="OO564_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6653458.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13280..14158
                     /gene="nadK"
                     /locus_tag="OO564_002356"
     CDS             13280..14158
                     /gene="nadK"
                     /locus_tag="OO564_002356"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6653459.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14244..15905
                     /gene="recN"
                     /locus_tag="OO564_002357"
     CDS             14244..15905
                     /gene="recN"
                     /locus_tag="OO564_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6653460.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16054..16395
                     /gene="bamE"
                     /locus_tag="OO564_002358"
     CDS             16054..16395
                     /gene="bamE"
                     /locus_tag="OO564_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6653461.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16457..16747)
                     /locus_tag="OO564_002359"
     CDS             complement(16457..16747)
                     /locus_tag="OO564_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6653462.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(16737..17174)
                     /gene="ratA"
                     /locus_tag="OO564_002360"
     CDS             complement(16737..17174)
                     /gene="ratA"
                     /locus_tag="OO564_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6653463.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17345..17827
                     /gene="smpB"
                     /locus_tag="OO564_002361"
     CDS             17345..17827
                     /gene="smpB"
                     /locus_tag="OO564_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6653464.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18042..18404
                     /gene="ssrA"
                     /locus_tag="OO564_002362"
     tmRNA           18042..18404
                     /gene="ssrA"
                     /locus_tag="OO564_002362"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18609..19838
                     /locus_tag="OO564_002363"
                     /pseudo
     CDS             18609..19838
                     /locus_tag="OO564_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         5081 a         5208 c         4676 g         4873 t
ORIGIN      
        1 cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc
       61 tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa
      121 cagtacgggt actgtactaa agtcacttaa ggaaacaaac atgaaacaca taccgttttt
      181 cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg
      241 cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc
      301 aacactatga catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat
      361 gaaaaattat tagcgttact ggcagaacgg cgcgaactgg ccgtcgaggt gggaaaagcc
      421 aaactgctct cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt gctggaaaga
      481 ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag
      541 ctcatcattg aagattccgt attaactcag caggctttgc tccaacaaca tctcaataaa
      601 attaatccgc actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt
      661 gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa
      721 tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt
      781 gaaaatacca gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg
      841 tcgattgttg gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcactact
      901 gatttatcca ccatcaatac ggtctacagc catccgcagc cattccagca atgcagcaaa
      961 ttccttaatc gttatccgca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg
     1021 gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc
     1081 actttgtacg gtttgcaggt actggagcgt attgaagcaa atcagcgaca aaacttcacc
     1141 cgatttgtgg tgttggcgcg taaagccatt aacgtgtctg atcaggttcc ggcgaaaacc
     1201 acgttgttaa tggcgaccgg gcaacaagcc ggtgcgttgg ttgaagcgtt gctggtactg
     1261 cgcaaccaca atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg
     1321 gaagagatgt tctatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca
     1381 ttgaaagagt taggggaaat cacccgttca atgaaggtat tgggctgtta cccaagtgag
     1441 aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc
     1501 gctggattat tactggcgat tgtcattcgc ctgacgcaat aacacgcggc tttcactctg
     1561 aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cagaaactgt caataaacgc
     1621 ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt
     1681 gattaacgcc agattacgct ctgacgacat aatgatgtcg gcataaagct gcggatcctg
     1741 agcaaacagt cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag
     1801 aagttgctca agctgaacat tttcttctgc caagtgcagc ccgtaagcaa aagtagcaaa
     1861 gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg
     1921 caaccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg
     1981 tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg
     2041 gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt
     2101 cactgatgcc agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac
     2161 ttgctcagta acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc
     2221 agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag
     2281 catcttctcg aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag
     2341 tgacggacaa agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac
     2401 ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg
     2461 acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct
     2521 tttcacctcg cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc
     2581 tttatcgact tcatcaattt gatcgcgtaa tgcagtcaat tcagcaacca taataaacct
     2641 cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg
     2701 catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac
     2761 tgcgcggttg ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa
     2821 taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac
     2881 ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc
     2941 ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt
     3001 taccaccgcg caggatcaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct
     3061 gccctgcctg gttaatgcca acaaaacagt gcggctgggc ggcggcgcgc atagcattaa
     3121 ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg
     3181 aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa
     3241 acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc
     3301 ccatattcac cagctcaagc aacaatttac gcgcgatctg cagcccggct tctacatcaa
     3361 aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt
     3421 tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagagctt
     3481 taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata
     3541 ctaccagcag acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag
     3601 caatctgggc ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca
     3661 ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttatgc atgatggcga
     3721 tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca
     3781 taaagtaaag ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc
     3841 gctttcaacc cttgcttctg tatatataaa tttacacccc tttgaaaaca atccgctatg
     3901 ctttgaaaaa ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg
     3961 ttagcggatg tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt
     4021 atgatgtcgg tatggaagat gcgatatcgg acagcgccat aaaagatgac gatgcattta
     4081 gcgattcaca ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa
     4141 cttgccagac cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct
     4201 gcaacaatgt tgaaaatgcc agccaactgc atgaagtatg gcaaaaaggg gctgatgaaa
     4261 atgccagcac aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt
     4321 tactcattgt gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat
     4381 gagcatcctg aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg
     4441 tttttctcac cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc
     4501 tcaagagcct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
     4561 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt
     4621 tagtaccgcc ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca
     4681 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
     4741 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
     4801 acaaaatacc gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt
     4861 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
     4921 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
     4981 cgtttaaaag agcattacgc aacatcagta tgaccagcat atttatcact atgatgttga
     5041 tctggttgct gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc
     5101 tggcactgac agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg
     5161 gccctgcagc aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga
     5221 agtacgtgat aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg
     5281 cgatactttc agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat
     5341 tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaattag
     5401 ccattttatc tggttttcac tcgccgtact gaccggttgt attctgctgg catcaggcat
     5461 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac
     5521 cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg
     5581 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
     5641 gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc
     5701 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa
     5761 ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat
     5821 caatgatacc tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt
     5881 agctgaattt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat
     5941 ggtgctctat gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca
     6001 aatctttaat ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat
     6061 tggttacgcg atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca
     6121 caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat
     6181 cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg
     6241 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
     6301 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
     6361 tgactacaaa ttgctcccgg aaagccagca acagctccaa accatggcag ccaaattggc
     6421 ctcgacaggg ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag
     6481 ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg
     6541 tggacaaatt ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc
     6601 cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac
     6661 cccttaaaac acgagcaaac agacaaaaaa agggccagcc aattgcccag cccttcttaa
     6721 caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca
     6781 gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa
     6841 atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa
     6901 attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc
     6961 tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta
     7021 tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata
     7081 atgttgctca taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca
     7141 tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg
     7201 cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc
     7261 ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg
     7321 gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc
     7381 cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc
     7441 gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc
     7501 cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc
     7561 gaccgcacac cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa
     7621 gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg
     7681 ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt
     7741 aaggacgatc gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct
     7801 ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc
     7861 ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt
     7921 atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga
     7981 ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat
     8041 ctttcaggtt tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcgacga
     8101 ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt
     8161 agtagtcccc ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg
     8221 tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat
     8281 tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc
     8341 agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc
     8401 cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg
     8461 cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt
     8521 tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc
     8581 gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa
     8641 cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga
     8701 acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg
     8761 tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa
     8821 aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc
     8881 atttagcgac caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc
     8941 attccgcctt tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg
     9001 cggttaacgt cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct
     9061 ttgatgattt ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata
     9121 cgcaccagca ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc
     9181 ggcagtttgc ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca
     9241 agaaagtcgt tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc
     9301 tgcgcgcggt caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg
     9361 agaatacgcg acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca
     9421 acaccgagga acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg
     9481 cgggcatcgc cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat
     9541 gcttttgccg tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca
     9601 accgggttaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
     9661 agacgaccag cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
     9721 gccgcgttaa caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc
     9781 acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa
     9841 accaccagca ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg
     9901 gttgttttac cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc
     9961 agattcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg
    10021 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
    10081 acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg
    10141 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
    10201 ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg
    10261 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
    10321 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
    10381 tttcttattg ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc
    10441 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
    10501 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
    10561 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
    10621 ttggtcagtt tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
    10681 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg
    10741 cccaatgaat acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta
    10801 tcgctctttt cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg
    10861 attgattacc aactgaagaa caagaagctg gcatttaacc aggaaatgcc gccattgatg
    10921 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg
    10981 ctttgcactg gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct
    11041 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa
    11101 ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg
    11161 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
    11221 ccctggaaca catttctact actacgttga tcattattct gatcatcatg gtggtcattt
    11281 cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac
    11341 atatggcgaa acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag
    11401 accgcctgat aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc
    11461 tcggcactat tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg
    11521 tgctgacttt tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt
    11581 acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga
    11641 tgccgctggt ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca
    11701 aaaccgatat cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg
    11761 aatcgcgctc acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg
    11821 aaaaaatgac cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca
    11881 acgatgactg gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc
    11941 tttaccgtga ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt
    12001 tgatgtcgga gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct
    12061 attttgtgcc tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa
    12121 agaaagtcgg cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag
    12181 atattctgga agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag
    12241 aggtcacgcc gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa
    12301 tcaacaaagc ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca
    12361 ttcttgaggc actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcgagtacg
    12421 atatcgatat tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga
    12481 aaccgctgcg cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc
    12541 gtgaattatt acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca
    12601 cgaatcgtac gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc
    12661 gcaacgtcat cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg
    12721 ttagtttcgg cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc
    12781 gtcagctcaa tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc
    12841 agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg
    12901 aatttgtggg ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct
    12961 tttacgcgca aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga
    13021 ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc
    13081 tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc
    13141 tgttctttac tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc
    13201 ttattatggg gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg
    13261 caacaaggac ctcggaaaaa tgaataatca tttcaagtgc attggcattg tgggacaccc
    13321 acggcaccct actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg
    13381 ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac
    13441 tggcacgctc gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg
    13501 taatatgctg ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa
    13561 ccgtggcaac ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc
    13621 cgatgtgctg gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg
    13681 tcagcaagat tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg
    13741 caaagtggcg catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca
    13801 gcgatctgat gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc
    13861 gggcggtcct attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca
    13921 tacgttgtca gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc
    13981 gcatcgccgt aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga
    14041 aggtgaagat gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga
    14101 ttacagttat ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt
    14161 ttacgccagc ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg
    14221 gtttttcata caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg
    14281 ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg
    14341 cgggtaaatc tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg
    14401 acatggtgcg taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata
    14461 cgccagcggc cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc
    14521 ttcgtcgcgt gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc
    14581 ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc
    14641 atcaattact caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa
    14701 cctctctact tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc
    14761 tcgcgcatca tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc
    14821 aattaaaaga acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag
    14881 agtacaaacg tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat
    14941 taatggccga cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg
    15001 tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag
    15061 ctaccatcca gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc
    15121 tcgatcccaa ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac
    15181 gtaaacatca cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac
    15241 agcagcaact ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac
    15301 atcatcagca ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag
    15361 aagaacttgc acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt
    15421 ttacgattga tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg
    15481 agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg
    15541 gtggtgaatt gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa
    15601 ccccggcact gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg
    15661 tcggcaaact gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc
    15721 cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga
    15781 tgacagaaac acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc
    15841 tgcttggtgg cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag
    15901 cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa
    15961 gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg
    16021 tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca
    16081 gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt
    16141 cctgacatca accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc
    16201 atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt
    16261 acgaatacct ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa
    16321 acgctgacgc tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg
    16381 ctgagtggta actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc
    16441 accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt
    16501 tcggatcggc aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc
    16561 ttagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac
    16621 gcaattccag caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg
    16681 tcactcgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac
    16741 ctagcactgt aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc
    16801 tctttaaaca cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga
    16861 tgaaactcga tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat
    16921 ttcttgaacg gcccatccac cagattcata agaatacttt ggttactggt caactggttg
    16981 cgggtagtaa acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc
    17041 ccaggagtgg actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac
    17101 tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg
    17161 ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct
    17221 gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc
    17281 ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac
    17341 gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg
    17401 cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg
    17461 ggaagttaaa tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg
    17521 tgacggagag gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca
    17581 tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc
    17641 attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa
    17701 aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg
    17761 ttcagatatc aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca
    17821 ccgttaaacc tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc
    17881 gcgaatgaac atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa
    17941 tcgaataaaa atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg
    18001 tcatggcgct cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg
    18061 acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca
    18121 aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc
    18181 tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc
    18241 gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg
    18301 cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga
    18361 tgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg
    18421 attaccagag tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt
    18481 tttgtgcccc gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta
    18541 ggcaccttgt taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa
    18601 acccggcaat ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac
    18661 ccaaggaagc ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca
    18721 gcgggagtaa aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga
    18781 aaagcatagg cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt
    18841 cccgctcact cctagctaaa cagattgagc cgcaggaaca tcagcaagaa caattgcgta
    18901 gttcgctgga agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga
    18961 agaaagccag tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg
    19021 tctttcctgc gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg
    19081 ccgtacaacc ggttcaggcc agaggagcac tggaaacggt tcgtcgccag tgtcaacgta
    19141 ttaatgaggt catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta
    19201 atatcggtaa agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc
    19261 agctatctca actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc
    19321 tgttcatgtg gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg
    19381 aagaagtaga catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc
    19441 gcgaccatac tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc
    19501 taagcggcaa ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca
    19561 gtcagactgt taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg
    19621 gtctgagatc aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg
    19681 aggcagcatt ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt
    19741 acctggaaca gcgtcgcccg atgatgcaat ggtaggcaaa ttttgtgatg gcagcggatc
    19801 gtggaagcat gattgaaggt gggataaaag gaatgaag
//
LOCUS       DAJTYI010000069        19811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYI010000069 DAJTYI010000000
VERSION     DAJTYI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1588)
                     /gene="glyS"
                     /locus_tag="OO564_002364"
     CDS             complement(<1..1588)
                     /gene="glyS"
                     /locus_tag="OO564_002364"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6653465.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFML"
     gene            complement(1598..2509)
                     /gene="glyQ"
                     /locus_tag="OO564_002365"
     CDS             complement(1598..2509)
                     /gene="glyQ"
                     /locus_tag="OO564_002365"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6653466.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(2604..2903)
                     /locus_tag="OO564_002366"
     CDS             complement(2604..2903)
                     /locus_tag="OO564_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6653467.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3078..4073
                     /gene="wecH"
                     /locus_tag="OO564_002367"
     CDS             3078..4073
                     /gene="wecH"
                     /locus_tag="OO564_002367"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6653468.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4115..4552)
                     /locus_tag="OO564_002368"
     CDS             complement(4115..4552)
                     /locus_tag="OO564_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6653469.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4598..4939)
                     /locus_tag="OO564_002369"
     CDS             complement(4598..4939)
                     /locus_tag="OO564_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653470.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5209..6561)
                     /gene="xylB"
                     /locus_tag="OO564_002370"
     CDS             complement(5209..6561)
                     /gene="xylB"
                     /locus_tag="OO564_002370"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6653471.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(6633..7955)
                     /gene="xylA"
                     /locus_tag="OO564_002371"
                     /pseudo
     CDS             complement(6633..7955)
                     /gene="xylA"
                     /locus_tag="OO564_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8321..9313
                     /gene="xylF"
                     /locus_tag="OO564_002372"
     CDS             8321..9313
                     /gene="xylF"
                     /locus_tag="OO564_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6653472.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9391..10932
                     /gene="xylG"
                     /locus_tag="OO564_002373"
     CDS             9391..10932
                     /gene="xylG"
                     /locus_tag="OO564_002373"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6653473.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            10910..12091
                     /gene="xylH"
                     /locus_tag="OO564_002374"
     CDS             10910..12091
                     /gene="xylH"
                     /locus_tag="OO564_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6653474.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12169..12699
                     /locus_tag="OO564_002375"
     CDS             12169..12699
                     /locus_tag="OO564_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6653475.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            12811..13347
                     /locus_tag="OO564_002376"
     CDS             12811..13347
                     /locus_tag="OO564_002376"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6653476.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13455..14279)
                     /locus_tag="OO564_002377"
     CDS             complement(13455..14279)
                     /locus_tag="OO564_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6653477.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            14599..16629
                     /gene="malS"
                     /locus_tag="OO564_002378"
                     /pseudo
     CDS             14599..16629
                     /gene="malS"
                     /locus_tag="OO564_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            16807..18060
                     /gene="avtA"
                     /locus_tag="OO564_002379"
     CDS             16807..18060
                     /gene="avtA"
                     /locus_tag="OO564_002379"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6653478.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18211..18684)
                     /locus_tag="OO564_002380"
     CDS             complement(18211..18684)
                     /locus_tag="OO564_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6653479.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(18786..19634)
                     /gene="yiaJ"
                     /locus_tag="OO564_002381"
     CDS             complement(18786..19634)
                     /gene="yiaJ"
                     /locus_tag="OO564_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6653480.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
BASE COUNT         5000 a         5016 c         4844 g         4951 t
ORIGIN      
        1 cgagcataaa gtcgataaca tcatcaacta cgttggcatt agtcagctta tcgccataca
       61 gacgcaccgc ttcttcggtc agcgtttgca gatcaaggtt gaggttcttc tcaacgataa
      121 ttcgcagcac gccaagtgcg gcacgacgca gcgcaaacgg gtctttgtcg cctttcggat
      181 gctgaccgat accgaagata cccgccagag tatccatctt atcagcaatc gccagcgcac
      241 aagctaccgg gttggacggc aggtcatcac cggcaaagcg cggctgatac tgctcattca
      301 gcgcgacggc tacgtcttcc gcttcgccat cgtgacgcgc gtagtgcatc cccataacgc
      361 cttgggtgtc ggtgaactcg aagaccatgt tggtcatcag gtcacactta gacagcaggc
      421 ccgcacgggt tgcgtggtta acgtcagcgc caatctgttc ggcgatccag ccagccagcg
      481 cctggatgcg gtcagtcttg tcacgcagcg tacccagctg ttgctggaac agcacggttt
      541 gcaggcgcgg caggttatct tccagacgtt ttttgcggtc ggtgttgaag aagaactcgg
      601 catccgccag acgtggacga acgactttct cgttaccaga gataatttgc tgcggatctt
      661 tcgattcgat gttggcaacg aagataaagt tcggcagcag tttgccgtcg ttcgcataca
      721 ccgggaagta cttctggtca cctttcatgg tgtaaaccag ggcttcagcc ggcaccgcga
      781 ggaatttctc ttcgaatttt gcggtcagaa cgaccggcca ctccaccagc gaagccactt
      841 cttccagcag gctttcgctt aagtcagcgt taccgccaat cttacgcgct gcttcttcgg
      901 catcggcttt aatcttcgct ttacgttctt cgtaatcggc gatgactttc ccgcgctcac
      961 gcagaatttc cggatactga tcggcgttgt cgatggtgaa ttccggttcg cccataaagc
     1021 ggtggccgcg aatcacgcga tcggactgaa tgcccagaat ggttgccgga atgactttgt
     1081 cgcccagcag cagggtcacg gtgtgcaccg gacgcacgaa gtgcacgtcg cttgcacccc
     1141 agcgcatcag tttcgggatc ggcagtttcg ccagcgaagt cgcaaccata ttcggcagca
     1201 gtgcttcggt gctttcgccc ttcacatggg cgcgatacag cagccattcg cctttatcgg
     1261 tagtcagacg ctcagcctgg tcaacggtaa taccacaacc acgcgcccaa ccttctgccg
     1321 ctttgctcgg ttttccttca gcgtcgaacg cctgggcaat cgccgggccg cgtttttcga
     1381 tttcacgatc tggttgtgct tccgccaggt tagctacttt cagcgccaga cgacgcggag
     1441 cagcaaacca ttgaacggtg ccgtgtgcga ggcctgcgtt atccagctcc gcagtaaagt
     1501 tcgcagcaaa ggactctgcc aggctgcgca gtgcttttgg tggcagctct tcagtgccga
     1561 tttccaccag aaaagttttc tcagacatag ccgcctctta cttatctttg ttgcacatcg
     1621 ggaagccgag ggcttcacgg gaagcgtaat atgcttctgc cactgctttg gtcagggtgc
     1681 gaatgcgcag aatgtagcgc tgacgctcgg tgacggagat ggctttacgc gcatccagca
     1741 ggttgaagct gtgggcggct ttcagaatac gctcgtaggc tggcagcggc agcggatttt
     1801 ccagcgccag cagctgctgc gcttctttct cgtactgctc aaagcaggtg aacaggaagt
     1861 ccacatccgc gtattcgaag ttgtaagtgg actgctccac ttcgttctga tggaacacgt
     1921 cgccgtaggt ggttttaccc agcgggccgt cgctccagac taggtcgtaa acgctgtcta
     1981 cgccctgaat gtacatggcc agacgttcca gaccgtaggt gatctcgccg gtaaccggtt
     2041 tacactccag accaccaacc tgctggaagt aagtgaactg cgtcacttcc atgccgttta
     2101 gccacacttc ccagcccagt ccccaggccc ccagagtcgg gttttcccag ttatcttcca
     2161 cgaaacggat gtcgtgaata gtcgggtcca tgcccagctc tttcagagaa ccgaggtaca
     2221 gctcctgaat attgtccggc gatggcttaa tgaccacctg gaactgatag tagtgctgta
     2281 aacggttggg gttttcgccg tagcgaccat cggtcgggcg acgagaaggc tgaacataag
     2341 cagccgccat cggttctggc cccagcgcgc gcagacaggt cattgggtga gaggttcccg
     2401 cgccgacttc catgtccaat ggttgaacaa tggtgcagcc ctggcgagcc cagtaatcct
     2461 gtaaggtcag gatcaagccc tggaaggtcc tggtatcaaa cttttgcata ttatttcgtg
     2521 ctggatacgt gtggatttaa aggaagggat cagtataccc gctggatgga agatatacag
     2581 tacgaaacgg gaaaaagcag ggcttaacgc atggaaagat gcaaaaaatg gccatctgcg
     2641 tcaaaagaac agacaaactg ctctttacgg aaggtataac cgcgcatttc atagcttccc
     2701 tggaactgtt cgaaggcggt gacgtcgatt ttttgctcgc cggtgttata acgttttgcc
     2761 gcctgatcct tgcataacgc ttccatattc aacgaacgca gaggatcaac ctttacccgt
     2821 tgtgctttct gcacgggtga cggtgtagaa caacccacta gcaccataag cgccattgcc
     2881 ggaaagaaag cgttcatcat cattaaatta ccgtctggtt tgcaggtgag tcttattatt
     2941 tatatcggta tagtggctga tatcttacga atttccttaa gcctctatag gctgtagtga
     3001 taaaactcag attattccag tgcgccaacc aaatgattta tcggtcagac tttttttaac
     3061 cagatacaga gacacagatg cagcccaaaa tttactggat tgataacctg cgagggatag
     3121 cgtgtttaat ggtggtgatg attcacacca ctacctggta tgtgaccaat gctcatagtg
     3181 ttagccccgt cacatgggat atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc
     3241 cgctattttt catgatttcc ggctatctct tttttggcga acgcagcgcc cagccgcgcc
     3301 atttcttgcg tatcggctta tgtctgattt tttatagcgc aatcgcactg ctctacattg
     3361 cgctgtttac ctccatcaat atggagttag cgctgaaaaa cctgctgcaa aagccagtgt
     3421 tttaccactt gtggtttttc ttcgcgattg cggtgattta tctggtttca ccgctgattc
     3481 aggtgaagaa cgtcggcgga aaaatgttgc tggtgctaat ggtggtgatt ggcattatcg
     3541 ctaacccaaa cacagtgccg cagaaaattg acggttttga atggctgcca attaacttat
     3601 atatcaatgg cgatactttt tactacattc tgtatggcat gttgggccgc gctataggga
     3661 tgatggacac acagcataaa gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg
     3721 tttttattat ctctcgcggg acattatatg aattgcagtg gcgcggaaat tttgccgata
     3781 cctggtatct ttactgtggg ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg
     3841 ttaaaaacac gctggatacg cgtaccattc gcggacttgg cttaatctcc cgccattcat
     3901 tgggtatata cggattccac gccttgatta tccatgcgct gcgcacccgg ggaattgagc
     3961 ttaaaaattg gccaatactg gatattattt ggatcttttg cgcgacgttg gcagcgagtt
     4021 tgttactttc tatgctggta caacgaatcg acagaaacag attagtgagt taagtaaaag
     4081 cccgttcaca ttggactgac cgggcttacg tgaattattc gctgtattct tcctgggtaa
     4141 cctgtgtttc ttttggcggg tttattccgg catcacgaat attcttctgc accgctactg
     4201 caccaaacaa gcttaagaag aaagccagtc cataaaaacc tttttcactg agtaataatg
     4261 tcgcattcca cagacctacc atcagtagag caacagagat aataaagaca gtcaggcagg
     4321 tcatataata aatggaagtg gtcggtatgc cttcatactt gtcccgaacg gtcttttgat
     4381 aagacgcagc agaaaacagt cctaacacca gtacggcaaa ataataccct ttttcattta
     4441 actgcatctc tgcattccat agccctaaca ggtaggtaac gataccacca acgagagcta
     4501 tccatgacac aatactaaag gccggtgaat aggttgatat tttgttgtcc atcacagtat
     4561 tcctttcatt cctgaatgtg taagagcttt atgttgctca taccgagcga aacaaacgca
     4621 tttgaccaaa caagcagaca aaaaaggcga tggggtagat caccatttca taagcattga
     4681 taggtgcatt taaaacaccg ataaacaaga gaccgatagt gattaacgca actaactggc
     4741 agatatgggt aaaaaaatta tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt
     4801 taccaaaagt tgctgtcatt aacacgccaa gaaaatagcc ttttccactt agcaatgggc
     4861 atgagatcca tagcccaacc agataaacca gcgccccgac aacaaataat agttttgcca
     4921 cagtttttga ggttttcata acgatctcca tatctaccag cgatacatta cgagtaacca
     4981 acgaaagaca aaactgaaaa atgccattaa caaatgattt tcagaataaa ttcatactaa
     5041 atattaatta attagtgaga tatatagatg tgaattatcc cccacccggt caggcagggg
     5101 ataacgttta cgccattaat ggcagaagtt gctgatagag gcgacggaac gtttcacgtc
     5161 gtggctgata agcggcataa cgctgcgcat ctggtagatg cgactgttct aacggtagct
     5221 gcggcaacaa ctcaatgagc gatttctctg gattcgccgc aatctgcgcc agccttgctg
     5281 cgcccagtgc tggccccaca tcccccccgt acggtaatcg agctgctgac cgctgatatc
     5341 cgccagcatc tgacgccagt actcactacg cgccccgcca ccaatcaacg taacactttg
     5401 cggtttaatg ccgcaggcat gcacgacatc catgccatct gccagcgcat aacccacgcc
     5461 ttccagcact gctcgcgcca gttcattggg gccatgttga tgagtcaaac caaagaaaac
     5521 ccccttcgcc tggggattat tgtgtggtgt acgctcgccg gaaagataag gcagaaacca
     5581 aactggctcg gcactttcat cagcctgttg agctgcagcg attaaagctg ggacattgca
     5641 caggccggtt aatttcgcgg cccaatccag acacgatgct gcactcagca ttacagacat
     5701 taaatgccaa cgctgcggta gcgcatggca aaagctgtgt acagcgcttt ctggcttgct
     5761 taagaaccct tcgctgacag caaaatagac ccccgacgtc cccagcgata acattgcctg
     5821 attagcatca accattccca caccaactgc accagctgca ttgtcgccac cgcctgcgac
     5881 aactggcacc gtcgccatac cccacgcttt cgcaacttca ggtaacaaag caccagtaat
     5941 ttcgctgcct tcgtataatg cgggcatctg gtcacgagat aagtcgcaag cctgcagcat
     6001 gacgtcactc cagtcacgct ttgcgacatc cagccacatg gtgccagctg cgtcagacat
     6061 atcgctggca aactcccccg tcatacgcag acgcaagtaa tctttcggta ataatacttt
     6121 gtcgatttga cggaatatct ccggctcatg ccgctgaacc catagcaatt taggcgcagt
     6181 aaatccgggc atcatcaggt tgccggtaat cactcgtgat tgcggaactc tcgcttccag
     6241 caaagtgcac tcttgcgcac agcgcccgtc gttccacaaa atggcagggc gcaatacccg
     6301 ttgttgagca tccagtaagg ttgctccatg catctggccg gcaataccca atgctttaac
     6361 gtcctgcaga gaatgctgat cgcccagagc tttcattgcg cgatcagttg cctgccacca
     6421 ctgttccggg tcttgttccg accaaagtgg atgcgggcgc gaaacggtca gcttttccgt
     6481 ttgcgaagca accacctcac cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc
     6541 aagatctatc ccgatataca tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg
     6601 ataaccgggc caacggactg cacagttagc cgttatttgt cgaacagata atgatttacc
     6661 agattttcca gttgctcctg gcgaccactc tgatgcaccg gagacaaatt atgttcctga
     6721 gcatatttgg ctaaatctgc cagtgacatt tggcctttca ggatttgctg gcccaattcg
     6781 ctattccagc cggaataacg ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg
     6841 cgcgctgcaa ttttcagcgc cagtgccatc gtatccatcg cgccgatatg accgtaaaac
     6901 agatcatatt tatcagtact ttgacgacgt actttggcat cgaagttcag accaccggtg
     6961 gtgaaaccgc ctgctttgag aatttcatac atcaccagcg cattctcttc cacactgttc
     7021 gggaactggt cggtgtccca gcccagttgc gcatcgccac ggttggcgtc gacagaaccg
     7081 aacaggccaa gcgcaatggc ggtggctatt tcatgatgga aagagtgacc tgccagcgtc
     7141 gcgtggttag cttcaatgtt cagtttaatc tctttttcca gaccaaactg tttcaggaag
     7201 ccatagaccg tcgcggcatc gtaatcatat tgatgtttag tcggttcttg cggtttcggt
     7261 tcgataagca acgtgcccta gaagccgatt ttatgtttat gctcaaccac catctgcata
     7321 aagcggccca gttgttcacg ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct
     7381 tcacgaccac cccacaggac atagttttca ccgcccaatt tatgggttgc ttccatcgct
     7441 gtaacaactt gcgttgccgc ccagctgaag acttcaggat ctgggttcgt cgccgcaccc
     7501 gcgccatagc gagggtttgt aaagcagtta gcggttcccc acagcagctt cacgccgctc
     7561 tcttcttgct tggctgccag gacatcaacc atttgcgcaa aattattgat gtactctttt
     7621 aacgacgcgc cctcagggga aacatccaca tcgtggaagc aataaaatgg cacatgtaac
     7681 ttgtggaaaa actcaaatgc gacatctgct ttacgcttcg ccaacgccag tgcctcacca
     7741 ggctgctgcc acggacgatt aaacgccccc acaccaaaca tgtccgcccc gttccagcag
     7801 aaggtgtgcc agtagcaggc ggcaaaacgc aagtgctctt ccatacgctt acccaacacc
     7861 agttcgtcgg gattgtagtg acggaatgct aacgggtttg aggattttga gccttcataa
     7921 cgaacgcgat cgagctggtc aaaataggct tgcatattga actccataat caggtaatgc
     7981 cgcgggtgat ggatgatgtc gtaatattgg gcactccctt tcaattgctc aattatgtta
     8041 tttcacactg ctattgagat aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat
     8101 gatgaaactg ggtaattcct cgaagataaa aatgcaataa gtacaattgc gcaacaaaag
     8161 taagatctcg gtcataaatc aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct
     8221 gtaattgttt tcccctgttt agttgctaaa aattggttac gtttatcgcg gtgattgtta
     8281 cttattaaag ctgtcctcta actacagaag gccctacacc atgaaaataa agaacattct
     8341 actcaccctt tgcacctcac ttctgcttac caacgttgcg gcacacgcca aagaagtcaa
     8401 aataggtatg gcgattgatg atctccgtct tgaacgctgg caaaaagatc gagatatttt
     8461 tgtgaaaaag gcagaatctc tcggtgcgaa agtatttgta cagtctgcaa atggcaatga
     8521 agaaacacaa atgtcgcaga ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat
     8581 tattccgtat aacggtcagg tattaagtaa cgttgtaaaa gaagccaaac aagaaggtat
     8641 taaagtatta gcttacgacc gtatgattaa cgatgcggat atcgattttt atatttcttt
     8701 cgataacgaa aaagtcggtg aactgcaggc aaaagccttg gtcgatattg ttccgcaagg
     8761 taattacttc ctgatgggcg gctcgccggt agataacaac gccaagctgt tccgcgccgg
     8821 acaaatgaaa gtgttaaaac cttacgttga ttctggaaaa attaaagtcg ttggtgacca
     8881 atgggttgat ggctggttac cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc
     8941 caataataac aaaattgatg ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat
     9001 tcaggcatta agcgcgcaag gtttatcagg gaaagtagca atttccggcc aggatgcgga
     9061 tctcgcaggt attaaacgta ttgctgccgg tacgcaaact atgacggtgt ataaacctat
     9121 tacattgctg gcaaatactg ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc
     9181 aaaagcagat acctcactga ataatggcct gaaagatgtc ccctcccgcc tcctgacacc
     9241 gatcgatgtg aataaaaaca acatcaaaga tacggtaatt aaagacggat tccacaaaga
     9301 gagagagctg taagcgttac gccccggcgc ggagcggggg cgtgatttct ctccatgccg
     9361 cgtgaatgaa ttggcttagg tggagtcgtt atgccttatc tacttgaaat gaagaacatt
     9421 accaaaacct tcggcagtgt gaaggcgatt gataacgtca gcttgcggtt gaatgctggc
     9481 gaaatcgtct cactctgtgg ggaaaatggg tctggtaaat caacgctgat gaaagtgctg
     9541 tgtggtattt atccccatgg ctcctacgaa ggcgaaatta tttttgcggg agaagagatt
     9601 caggcgagtc acatccgcga taccgaacgc aaaggtatcg ccatcattca ccaggaattg
     9661 gccctggtga aagaattgac cgtgctggaa aatatcttcc tgggtaacga aataacccac
     9721 aatggcatta tggattatga cctgatgacg ctacgctatc agaagctgct cgcacaggtc
     9781 agtttatcca tttcacctga tacccgcgtt ggcgatttag ggcttgggca acaacaactg
     9841 gttgaaattg ccaaggcact taataaacag gtgcgcttgt taattctcga tgaaccgaca
     9901 gcctcattaa ctgagcagga aacgtcggtt ttactggata ttattcgcga tctacaacag
     9961 cacggtatcg cctgtattta tatttcgcac aaactcaacg aagtcaaagc gatttccgat
    10021 acgatttgcg ttattcgcga cggacagcac attggtacgc gtgatgctgc cggaatgagt
    10081 gaagacgata ttatcaccat gatggtcggg cgagagttaa ccgcgcttta ccctaatgaa
    10141 ccacatacca ccggagatga aatattacgt attgaacatc tgacggcatg gcatccggtc
    10201 aatcgtcata ttaaacgagt taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg
    10261 ggtattgccg gactcgttgg tgccggacgt accgagacca ttcagtgcct gtttggcgtg
    10321 tggcccggac aatgggaagg aaaaatttat attgatggca aacaggtaga tattcgtaac
    10381 tgtcagcaag ccatcgccca ggggattgcg atggtacccg aagacagaaa gcgcgacggc
    10441 atcgttccgg taatggcggt tggtaaaaat attaccctcg ccgcactcaa taaatttacc
    10501 ggtggcatta gccagcttga tgacgcggca gagcaaaaat gtattctgga atcaattcag
    10561 caactcaaag ttaaaacgtc gtcccccgac cttgctattg gacgtttgag cggcggcaat
    10621 cagcaaaaag cgatcctcgc ccgctgtctg ttacttaacc cgcgcatcct cattcttgat
    10681 gaacctacca ggggtattga tattggcgcg aaatatgaga tctacaaatt aattaaccaa
    10741 ctcgtccagc agggtattgc cgttattgtc atctcttccg aattacctga agtgctcggc
    10801 cttagcgatc gtgtactggt gatgcatgaa gggaaactaa aagccaacct gataaatcat
    10861 aacctgactc aggagcaggt gatggaagcc gcattgagga gcgaacatca tgtcgaaaag
    10921 caatccgtct gaagtgaaat tggccgtacc gacatccggt ggcttttctg ggctgaaatc
    10981 actgaatttg caggtcttcg tgatgattgc agctatcatc gcaatcatgc tgttctttac
    11041 ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc tccaacctgt tacgccagac
    11101 cgcgattacc ggcatcctcg cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga
    11161 cctttccgtc ggctcaatga tggggctatt aggtggcgtc gcggcgattt gtgacgtctg
    11221 gttaggctgg cctttgccac ttaccatcat tgtgacgttg gttctgggac tgcttctcgg
    11281 tgcctggaac ggatggtggg tcgcgtaccg caaagtccct tcatttattg tcaccctcgc
    11341 gggcatgttg gcatttcgcg gcatactcat tggcatcacc aacggcacga ctgtatcccc
    11401 caccagcgcc gcgatgtcac aaattgggca aagctatctc cccgccagta ccggcttcat
    11461 cattggcgcg cttggcttaa tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg
    11521 tcaggctttg ggtttacagt ctccggcctc taccgcagta gtcggtcgcc aagctttaac
    11581 cgctatcatc gtattaggcg caatctggct gttgaatgat taccgtggcg ttcccactcc
    11641 tgttctgctg ctgacgttgc tgttactcgg cggaatgttt atggcaacgc ggacggcatt
    11701 tggacgacgc atttatgcca tcggcggcaa tctggaagca gcacgtctct ccgggattaa
    11761 cgttgaacgc accaaacttg ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg
    11821 attaatcctt agttctagac ttggcgctgg ttcaccttct gcgggaaata tcgccgaact
    11881 ggacgcaatt gcagcatgcg tgattggcgg caccagcctg gctggcggtg tgggaagcgt
    11941 tgccggagca gtaatggggg catttatcat ggcttcactg gataacggca tgagtatgat
    12001 ggatgtaccg accttctggc agtatatcgt taaaggtgcg attctgttgc tggcagtatg
    12061 gatggactcc gcaaccaaac gccgttcttg attttgataa aaattttctc aaagccggtt
    12121 acgtattacc ggttttgagt ttttgcatga ttcagcagga aaagaaccat gtttactaaa
    12181 cgtcaccgca tcacattact gttcaatgcc aataaagcct atgaccggca ggtagtagaa
    12241 ggcgtagggg aatatttaca ggcatcacaa tcagaatggg atattttcat tgaagaagat
    12301 ttccgcgccc gcattgataa aatcaaggac tggttaggag atgtcgtcat tgccgacttc
    12361 gacgacaaac agatcgagca agcgctggct gatgtcgacg tccccattgt tggggttggc
    12421 ggctcgtatc accttgcaga aagttaccca cccgttcatt acattgccac cgataactat
    12481 gcgctggttg aaagcgcatt tttgcattta aaagagaaag gcgttaaccg ctttgctttt
    12541 tatggtcttc cggaatcaag cggcaaacgt tgggccactg aacgcgaata tgcatttcgt
    12601 cagcttgtcg ccgaagaaaa gtatcgcgga gtggtttatc aggggttaga aaccgcgcca
    12661 gagaactggc aacacgcgca aaatcggctg gcagactgac tacaaacgct gccaccgcaa
    12721 accgggatta ttgccgttac tgacgcccga gcgcggcata ttctgcaagt atgtgaacat
    12781 ctacatattc ccgtaccgga aaaattatgc gtgattggca tcgataacga agaactgacc
    12841 cgctatctgt cgcgtgtcgc cctttcttcg gtcgctcagg gcacgcggca aatgggctat
    12901 caggcggcaa aactgttgca tcgattatta gataaagaag aaatgccgct acagcgaatt
    12961 ttggtcccac cagttcgcgt cattgaacgg cgctcaacag attatcgctc gctgaccgat
    13021 cccgccgtta ttcaggccat gcattacatt cgtaatcacg cctgtaaagg gattaaagtg
    13081 gatcaggtac tggatgcggt cgggatctcg cgctccaatc ttgagaagcg ttttaaagaa
    13141 gaggtgggtg aaaccatcca tgcaatgatt catgccgaga agctggagaa agcgcgcagt
    13201 ctgctgattt caaccacctt gtcgatcaat gagatatcgc aaatgtgcgg ttatccatcg
    13261 ctgcaatatt tctactctgt ttttaaaaaa gcatatgaca caacgccaaa agagtatcgc
    13321 gatgtaaata gcgaggtcat gttgtagcga tgatgagaat tgtcggcgtc acatcaggga
    13381 actcagcaca gccacctgat gcgagcaaaa gccttatccg accgacaaat cagataaggc
    13441 tcgaggaaat gaaattacat atgcgccgcg attaaccgtt ggttatcctg gtacattgcg
    13501 aacaggtagt tgttataact cttccctttg gtcgagtagc ccttcagctt gtgaatcatc
    13561 gctgtggcag tcacttcctg atccgcttta cgcagctgtg cacgcgattt acggaacgaa
    13621 gagtaagccg ggtgcgtatt caggttagtg acataggcgc tcaccgattc tttgacagaa
    13681 ctaaactgtg agtacccttt cactttaccc ggcgcattgg tacaacgtcc tttcatgcat
    13741 ttcatgccga acagattgtt attgttgcgc gccagcttcg acgttcccca accgctttct
    13801 gctgcagcca tcgtcgccac catactggta gggataatgt ctacgcgttc aagcaaggta
    13861 ttccacggga tttttcgcgt attaccagac cacttcacct tgtagcgttt ggcgatgtct
    13921 ttcagacgcg cacgctcagc aggtgaccat tggccctgat actgttttga aatcagccag
    13981 ttacgttctg cagtaatggc tgcattttgg ctggtaatgt aaggcattac ggtccggaga
    14041 aacgcctttt tccttggtgt tccggaaggg tattttcgca aatcaggaag tgaactactc
    14101 tttgcactat tgcgagaata ctcttgttta ctgcttacct gtttattact agctttagtt
    14161 aagtgggact tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac
    14221 accagagtga gtaacataag aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc
    14281 aggtctcctg gccggattta atcattccaa caccttatat ttttcacaaa tttgagagtt
    14341 gaatctcaaa tcatatcaaa aatagctgtc aagagcaccc caaggaatag tccaaatctg
    14401 aaactatgtc acgagttaac gattcagatt ggcgctaaat cgcagaaaat gtgggggtta
    14461 tcgcaaaatt cagccgtttt ttgcgcgcga tcgctcaccc ttgcttctca tcctgtggac
    14521 ttaccgctca gggatgagtt ttgtctggct tatcgctggc aaactgtctg aaatcgcagc
    14581 aataaggact catccaccat gaaactcgcc gcctgttttc tgacactcct tcctggcttc
    14641 gccgttgccg ccagctggac ttctccgggg ttccctgcct ttagcgaaca gggaacggga
    14701 acatttgtca gccacgcgca gttgcccaaa ggtacgcgtc cactcacgct aaattttgac
    14761 caacagtgct ggcagcctgc agatgcgata aaactcaatc agatgctttc cctgcaacct
    14821 tgtagcaaca cgccgcctca atggcgattg ttcagggacg gcaaatatac gctgcaaata
    14881 gacacccgct ccggtacgcc aacattgatg atttccatcc agaacgccgc cgaaccggta
    14941 gcaaacctgg tccgtgaatg cccgaaatgg gatggattac cgctcacgct ggatgtcagc
    15001 gccactttcc cggaaggagc cgccgtacgg gattattaca gccaacaaat tgcgatagtg
    15061 aagaacggtc aaataacgtt acaacccgct gctaccagca acggtttact cctgctggaa
    15121 cgggcagaaa ctgacacatc cgcccctttc gactggcata acgccacggt ttactttgtg
    15181 ctgacagatc gtttcgaaaa cggcgatccc agtaatgacc agagttacgg acgtcataaa
    15241 gacggtatgg cggaaattgg cacttttcac ggcggcgatt tacgcggcct gaccaacaaa
    15301 ctggattacc tccagcagtt gggcgttaat gctttatgga taagcgcccc atttgagcaa
    15361 attcacggct gggtcggcgg cggtacaaaa ggcgatttcc cgcattatgc ctaccacggt
    15421 tattacacac aggactggac gaatcttgat gccaatatgg gcaacgaagc cgatctacgg
    15481 acgctggttg atagcgcaca tcagcgcggt attcgtattc tctttgatgt cgtgatgaac
    15541 cacaccagct atgccacgct ggcggatatg caggagtatc agtttggcgc gttatatctt
    15601 tctggtgacg aagtgaaaaa aacgctgggt gaacgctgga gcgactggaa acctgccgcc
    15661 gggcaaacct ggcatagctt taacgattac attaatttca gcgacaaaac aggctgggat
    15721 aaatggtggg gaaaaaactg gatcagaacg gatatcggcg attacgacaa tcctggattc
    15781 gacgatctca ctatgtcgct ggcctttttg ccggatatca aaaccgaatc aactaccgct
    15841 tctggtctgc cggtgttcta taaaaacaaa acggatactc acgctaaagt catcgaaggc
    15901 tttacacctc gcgattactt aacccactgg ttaagtcagt gggtccgcga ctatgggatt
    15961 gatggttttc gggtcgatac cgccaaacat gttgagttgc ccgcctggca gcaactgaaa
    16021 accgaagcca gctccgcgct tcgcgaatgg aaaaaaacta accccgacaa agcattagat
    16081 gacaaacctt tctggatgac cggtgaagcc tagggccacg gcgtgatgca aagtgactac
    16141 tatcgccacg gcttcgatgc gatgatcaat ttcgattatc aggagcaggc ggcgaaagca
    16201 gtcgattgtc tggcgcagat ggatacgacc tggcagcaaa tggcggagaa attgcagggt
    16261 ttcaacgtgt tgagctacct ctcgtcgcat gatacccgtc tgttccgtga agggggcgac
    16321 aaagcagcag agttattact attagcgcca ggcgcggtac aaatctttta tggcgatgaa
    16381 tcctcgcgtc cgttcggtcc tacaggttct gatccgctgc aaggtacacg ttcggatatg
    16441 aactggcagg atgttagcgg taaatctgcc gccaacgtcg cgcactggca gaaaatcagc
    16501 cagttccgcg cccgccatcc cgcaattggc gcgggcaaac aaacgacact ttcgctgaag
    16561 cagggctacg gctttgttcg tgagcatggc gacgataaag tgctggtcat ctgggctggg
    16621 caacagtgac ttttccggct tcccgttcgt cagtacctcg ggaagccgcc aaccaggata
    16681 aaatgtcagc cctaatcagc gttgcgggat aaagcaccgc tcactcttca acagaccgat
    16741 ttgcacccca gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat
    16801 cccgctatga cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg
    16861 ctgatggaag atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt
    16921 aatccggcgc agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg
    16981 gaaagtggca aagcgactga tgcactgtgt aactacgacg gtccacaggg ggaaacggag
    17041 ctactcacac tgcttgccgg aatgctgcgc gagaagttgg gttgggatat cgaaccacag
    17101 aatattgcac taacaaacgg cagccagagc gcgtttttct acttatttaa cctgtttgcc
    17161 ggacgccgtg ccgatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt
    17221 ggctacgctg acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa
    17281 ctgctgccgg aaggccagtt taaataccac gtcgattttg agcatctgca tattggcgaa
    17341 gaaaccggga tgatttgcgt ctcccggccg acgaatccaa caggcaatgt gattactgac
    17401 gaagagttgc tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat
    17461 aacgcttatg gcgtcccgtt cccgggtatc atcttcagtg aagcgcgccc gctatggaat
    17521 ccgaatatcg tactgtgcat gagtctttcc aagctggggc tgcctggctc ccgctgcggc
    17581 attatcatcg ccaatgaaaa aatcatcact gccatcacca atatgaacgg cattatcagc
    17641 ctggcacctg gcggtattgg tccggcgatg atgtgtgaaa tgattaagcg taacgatctg
    17701 ctgcgcctgt ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc
    17761 gccatcattc gccgctattt accggaaaat cgctgcctga ttcataaacc ggaaggagcc
    17821 attttcctct ggctatggtt taaggatttg cccattacga ccgagcagct ctatcagcgc
    17881 ctgaaagcac gcggcgtgct gatggtgccg gggcacaact tcttcccggg actggataaa
    17941 ccgtggccgc atacgcatca atgtatgcgc atgaactacg taccagagcc ggagaaaatt
    18001 gaggcggggg tgaagattct ggcggaagag atagaaagag cttgggcaga aagtcactaa
    18061 aaattgcctg atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg
    18121 gaagaatttg taggccggat aaggcgttta cgccgcatct ggcataaaca aagcgcacta
    18181 ttccggcgtt gagaaacgcc ggaaaacgtt tcaaacaggc tgctgctgta gccgatgccg
    18241 ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc
    18301 cggccccgtc tcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga
    18361 agaaacgacc tccatcgcac caaacgggca agccagcata cagcttttac agccaatgca
    18421 acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac
    18481 attcgcgcac ggtgcatctt cacactgatg acaggctact ggcgtggtcc agctgtggtc
    18541 tttaatgaca cgaatacggg aaataaactc gtctggtgac aacgcagcgc aatcctgatt
    18601 ctcctgatgc gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc
    18661 atccgcaata ataaaccggt tcatgatcat tcctttgcac taaaacagca gagtgccaga
    18721 gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca
    18781 aagcgttatg tgagcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata
    18841 gcctgcgcgg tttcacgcag tggtttcagg agatttttct ctcccacctg tttcagacgt
    18901 gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga
    18961 acagcaatac aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca
    19021 cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gtgggttaac
    19081 ggctggatct catgctgatg gttttcccag tatgacttca cgtagtccgg gtgaccaaac
    19141 gccatgtaga tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca
    19201 cgggttcgca gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct
    19261 tcgcggctgg agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga
    19321 gcggcgatat gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg
    19381 gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca catagccgca ggactgtaat
    19441 ccctgcaata agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc
    19501 aacggacaac cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga
    19561 ctctggcttc cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc
    19621 acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat
    19681 ctcgacagat aatttataac cacttgattt ttatgtcttt tgaaattcat caatcagatt
    19741 gctttgttaa aaagtgatcg atatatttga aatctagttt cgcatattga aattttaaga
    19801 caaaaaagcg a
//
LOCUS       DAJTYI010000070        19310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYI010000070 DAJTYI010000000
VERSION     DAJTYI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(47..139)
                     /locus_tag="OO564_002382"
     tRNA            complement(47..139)
                     /locus_tag="OO564_002382"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO564_002383"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO564_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6653481.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO564_002384"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO564_002384"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6653482.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO564_002385"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO564_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6653483.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO564_002386"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO564_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6653484.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO564_002387"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO564_002387"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6653485.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO564_002388"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO564_002388"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6653486.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="OO564_002389"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="OO564_002389"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6653487.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="OO564_002390"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="OO564_002390"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6653488.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="OO564_002391"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="OO564_002391"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6653489.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="OO564_002392"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="OO564_002392"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6653490.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="OO564_002393"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="OO564_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6653491.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="OO564_002394"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="OO564_002394"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6653492.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="OO564_002395"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="OO564_002395"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6653493.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO564_002396"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO564_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6653494.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="OO564_002397"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="OO564_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6653495.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="OO564_002398"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="OO564_002398"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6653496.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO564_002399"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO564_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6653497.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19246..>19310)
                     /locus_tag="OO564_002400"
     CDS             complement(19246..>19310)
                     /locus_tag="OO564_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="involved in electron transport from formate to
                     hydrogen; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6653498.1"
                     /translation="DRNKLEQLSAEKRRRTALMF"
BASE COUNT         4530 a         5370 c         4935 g         4475 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc ctgccggaca
    19141 cgacagcgtc gtatccggca gtctcaggtc ggcgataccg cccgctccgt attctacgaa
    19201 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    19261 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga
//
LOCUS       DAJTYI010000071        19290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYI010000071 DAJTYI010000000
VERSION     DAJTYI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OO564_002401"
     CDS             complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OO564_002401"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6653499.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OO564_002402"
     CDS             complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OO564_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6653500.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OO564_002403"
     CDS             complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OO564_002403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6653501.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OO564_002404"
     CDS             complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OO564_002404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6653502.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OO564_002405"
     CDS             complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OO564_002405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6653503.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OO564_002406"
     CDS             complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OO564_002406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6653504.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OO564_002407"
     CDS             complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OO564_002407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6653505.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OO564_002408"
     CDS             complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OO564_002408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6653506.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OO564_002409"
     CDS             complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OO564_002409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6653507.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OO564_002410"
     CDS             complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OO564_002410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6653508.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OO564_002411"
     CDS             complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OO564_002411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6653509.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OO564_002412"
     CDS             complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OO564_002412"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6653510.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OO564_002413"
     CDS             complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OO564_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6653511.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OO564_002414"
     CDS             complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OO564_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6653512.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OO564_002415"
     CDS             complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OO564_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6653513.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15348..16340
                     /gene="asnA"
                     /locus_tag="OO564_002416"
     CDS             15348..16340
                     /gene="asnA"
                     /locus_tag="OO564_002416"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6653514.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OO564_002417"
                     /pseudo
     CDS             complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OO564_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OO564_002418"
     CDS             complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OO564_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6653515.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4605 a         5107 c         4838 g         4740 t
ORIGIN      
        1 ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccagg
       61 ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc
      121 agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg
      181 tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata caattgaccg
      241 ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc gttgttcggt
      301 gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg tttcagctcg
      361 cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa tgcgccttcc
      421 agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt gtcagagaaa
      481 tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat acggctcggc
      541 agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga
      601 gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac
      661 gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga
      721 gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga
      781 cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg
      841 ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga
      901 atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca
      961 caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc
     1021 tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt tatcgcgttc
     1081 ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca
     1141 tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg
     1201 tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat aaagcgacgg
     1261 gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca
     1321 atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg
     1381 atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga
     1441 gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca
     1501 gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg
     1561 atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa
     1621 ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg cagaggatgt
     1681 tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg agtcatatga
     1741 ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc cagacgacgt
     1801 aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac
     1861 atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc
     1921 gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt
     1981 atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg
     2041 gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc
     2101 cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg
     2161 gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt
     2221 tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa
     2281 tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc agacgcgctt
     2341 ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca ccaggacgca
     2401 gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc acaacggtat
     2461 acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga
     2521 ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa
     2581 catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa
     2641 cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga
     2701 gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga
     2761 tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag
     2821 cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg gcaatcagaa
     2881 tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa
     2941 tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt
     3001 tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc tggagtgttt
     3061 cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga
     3121 aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt
     3181 taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt
     3241 gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc
     3301 cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg
     3361 caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac
     3421 gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg
     3481 ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca
     3541 cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt
     3601 tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat cagcaggatc
     3661 tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg tttccagact
     3721 ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca
     3781 gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag
     3841 agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat
     3901 cctggccgcg aattgcagtg tcggccagaa cggtcacgtt gccaggctgc acttcaagaa
     3961 tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac
     4021 caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc
     4081 tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga
     4141 cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc
     4201 tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc
     4261 gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt
     4321 acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga agcgctggat
     4381 cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat
     4441 ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt
     4501 gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg agtccagcgg
     4561 gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc
     4621 aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag gtacgtatac
     4681 cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct gcagaacgcc
     4741 catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc
     4801 ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc
     4861 acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg
     4921 gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga taacgttgga
     4981 gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa
     5041 cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca tgtttacggt
     5101 tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat
     5161 caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta
     5221 ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc
     5281 gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta ccgggacttc
     5341 gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat
     5401 tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc
     5461 attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc
     5521 gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct
     5581 acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta
     5641 atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca
     5701 ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca
     5761 accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc
     5821 ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat
     5881 gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg
     5941 ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt
     6001 accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac ctgggcaaca
     6061 acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg
     6121 cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta
     6181 atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta
     6241 acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc aaggtgacca
     6301 atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta
     6361 cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg
     6421 cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc
     6481 ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga
     6541 ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt
     6601 gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg aagctgccaa
     6661 ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact
     6721 gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc actttctgac
     6781 cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg
     6841 ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct
     6901 tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa
     6961 taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt
     7021 tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc agtgcggtca
     7081 gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa
     7141 cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc gggaatgctt
     7201 cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag
     7261 acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcgcccattg
     7321 cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg gttgctacca
     7381 caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg
     7441 tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga
     7501 tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg atcaattcac
     7561 gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc tgtaccggct
     7621 gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt
     7681 cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc acacggccca
     7741 gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt
     7801 cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc tcgaggttca
     7861 gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca
     7921 ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt
     7981 cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat
     8041 tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac
     8101 cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct gccattacag
     8161 acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag
     8221 aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct acctcagcgg
     8281 tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg agcgcgttaa
     8341 gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac
     8401 cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct
     8461 ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt
     8521 ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg
     8581 taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat
     8641 gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc
     8701 caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc
     8761 agcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc
     8821 cagaatctgc gagcggagtt tattcgcctg ctcgatgatt acctgggctt ccgctttcgc
     8881 ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc
     8941 tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg
     9001 cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag
     9061 gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct
     9121 ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta
     9181 cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca
     9241 gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg aggatgccga
     9301 taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca
     9361 gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg
     9421 ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag
     9481 atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc
     9541 cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac
     9601 atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct taccccttca
     9661 aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc
     9721 gtgaagccgc cgatgccttt cattttgatg ttgtagaaca gaatcaggat aaatacgccc
     9781 agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga
     9841 cccagtacat gttcagcaat gtacggcagc aagtcgatag gcagtaaatc catcagattc
     9901 atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca
     9961 tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga
    10021 aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa aaccaagaac
    10081 aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct
    10141 ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg
    10201 tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt
    10261 tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga
    10321 tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg
    10381 ccagaacttt gaaagcttcg ccaaatgcga atgtccaagc cacccggcct ttcgctggtg
    10441 tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca
    10501 ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac
    10561 ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga
    10621 gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata
    10681 aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata cggtgcgccc
    10741 ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg
    10801 catacaattg cgacttttcc gctacccctg ttcgatcatg aaaaactgta aataacgcgt
    10861 aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat
    10921 ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta aaaacagata
    10981 ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt taataatgtt
    11041 ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt tcactaatga
    11101 cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag agtgaacgtg
    11161 gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca gtctaaccgg
    11221 tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat caccaccaga
    11281 tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac ctgatattct
    11341 tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta gaaacggcct
    11401 tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc aaaagcgcgg
    11461 ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct ctgtactggt
    11521 tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac gcgtttacca
    11581 aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg aatgcctggc
    11641 agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg tgccaccaca
    11701 atgctatcga gaatatggcg tactagcatc tcattaggat cgcggaccga agtcaggttg
    11761 tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt tttctggtga
    11821 tcagtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag cacggtgatt
    11881 acctgttctt gatgcgttgc ttggtaagcg ggtgcttacc aggcattttt aatgcgttat
    11941 gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga gatggccgca
    12001 ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ccggtttgtg atcgttaagt
    12061 ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt cgcgggtagc
    12121 agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc gatataacct
    12181 tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa cgcaggggca
    12241 aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag cagatcttca
    12301 ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc tgcagcttcc
    12361 gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat attctcaagt
    12421 ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg accgatttca
    12481 gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc gcgcgaagta
    12541 aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag tacgccgaga
    12601 tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg ggcagcgtta
    12661 agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc gttaatctga
    12721 ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag gtcgcgagga
    12781 tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt ttccatcccc
    12841 tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga gataccgttc
    12901 ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg ctgatttctg
    12961 tctgcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc gacaccttcg
    13021 atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc atgggttttc
    13081 tcgttggtat gagtgatata acacggcacc tgctggggat gcagggacgc attgcccata
    13141 aacgagaata ccggcattgg gttatcgcca tgttgttgcg ccagcacgct aaagtcgatg
    13201 gttcgagcat caatacgcgg cggagtaccg gttttcagac gaccaacgcg cagcggcagt
    13261 tcacgcaaac ggcgagagag cggaatggat ggcggatcgc cagcacggcc accgctgtaa
    13321 ttatccagac cgatatgaat tttgccgtcg aggaacgtcc caacggtgag caccacagct
    13381 ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc gttttcgaca
    13441 ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc cagcgccgta
    13501 cgtaccgcct gacggtagcg cacacgatcc gcctgagctc gggtagcgcg aaccgccggt
    13561 cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc tttcgccatc
    13621 agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc gccgatcgcc
    13681 gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag agtctgttga
    13741 cccatacgcg ccgcagccat cgcggcctcg gtgcctgcat gacccccgcc aatgatgatg
    13801 acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt atgaaaatgg
    13861 attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga cctgggatcc
    13921 tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat ctcttattag
    13981 gatcgcactg ccctgtggat aacaaggatc cgacttttaa gatcaacaac ctggaaagga
    14041 tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag aatgaggggt
    14101 tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg ttgatccaag
    14161 cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt gagtaaatta
    14221 atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg
    14281 atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc ctcgagttta
    14341 tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc gccaaagcgg
    14401 accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc
    14461 ggaatatctc cggcaccgtg ggtggagctg ataaccagcc agatccccga ggcagataaa
    14521 tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca
    14581 gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat aagagtgata
    14641 tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga acgcgttggg
    14701 atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgatg gtacgcatga
    14761 tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt gtttggatct
    14821 tgttgataag tacatgctgg agagcgtcga tcgaacggca cataactttt ataaagatgc
    14881 tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc agctttgcca
    14941 gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag cctacgtcat
    15001 aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc gcctgcttca
    15061 ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc gccagttcgg
    15121 cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc agattgtcga
    15181 tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta ttttagcctt
    15241 cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg catgtgtgtc
    15301 ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt
    15361 acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc caactggaag
    15421 aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc
    15481 aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg
    15541 cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta gggcaacacg
    15601 acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc
    15661 gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc gtaatgggcg
    15721 acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg gcgggaatta
    15781 aagcaaccga agctgcggtt aacgaagagt ttggcctggc accgttcctg ccggatcaga
    15841 ttcacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat gccaaagggc
    15901 gtgagcgggc gattgcgaaa gatcttggcg cggtattcct cgtcgggatt ggcggcaagt
    15961 tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta tgatgactgg agcaccccgt
    16021 cagagctggg ccatgcgggc ctgaacggcg atattctggt gtggaacccg gtactggaag
    16081 atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgctg aagcatcagc
    16141 tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg
    16201 gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact atgctgctgc
    16261 tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagttgct gttcgcgaga
    16321 gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat
    16381 cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc
    16441 cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt tcactttact
    16501 cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg catcggcatc
    16561 aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg caagatcggt
    16621 gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga
    16681 aagctcataa cggacgatct cggtggaaaa tagcataata taacagcgcc ggttttcagc
    16741 gagagcaatg cgcatcaaag ccaggcagaa cgctttcgca cactgttcat taaagccgcc
    16801 cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt catcgtaatc
    16861 tttatgtacc accggacgtt cgagcacttt ttcacgccac gactcaccgt gcaggcgata
    16921 ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt ccgttatccc
    16981 tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt gcagaccatc
    17041 aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt ccatctacgc
    17101 atcgttgcgc ggtattgatt tggcttcccg agaacgcccc ggctgctctg ccaggcgttt
    17161 cagttccggc tgttcgttaa gaaattcacc gtatttcaca ataaactgat agtcgccacg
    17221 tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt tatctgcgag
    17281 aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta acagttgttc
    17341 gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca gacttaaacg
    17401 ccaacgctgg agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag catcaaccaa
    17461 ctgccgggct tgttctgccc atggggaatt cagacgatgc agtaagtcta ggatctgtgg
    17521 tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc acatcacctc
    17581 ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg cctcacgcca
    17641 gcggggaaca tcatcagtga ttgccgcctt cagtcgtggg aatttttcaa agaagaccgc
    17701 cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct cttcgctgac
    17761 ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg aatctgtgca
    17821 ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat aaacagacat
    17881 ttttgctgtt cgctgaataa agcgtgttgc ttacgccagt cgctttcaag ctcttccagt
    17941 tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc cagcgtactg
    18001 ccttgtaaac ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc cagattcagt
    18061 ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt actcagccac
    18121 tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg cagttttaac
    18181 ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag ttgatactgc
    18241 tgacgacggc tgaaaatgcc gcccagacga attaacgtta aggctgtttt atcgctttgt
    18301 tgctgctgga gttgcaggtg acgttgcaca atcgcgccca ggcgggtcaa catcccttgc
    18361 tgttgccagg catgaccggt catcaataca tcaatttgtt gttgtatcaa attcaggctt
    18421 tgcgcgtcat accacaggca atctttcagc aaaatgagat caaccggggc aacagcactg
    18481 cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca gcgacgatcc
    18541 gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa aatcagctca
    18601 aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc atattcttca
    18661 tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg actggtcagc
    18721 atggagcgga aattcgcttt atcttgcact ttatctaacc atagacgaat cagcatgcgg
    18781 tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc cgccaccagt
    18841 agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc gttaatggcg
    18901 gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag aaagacgatt
    18961 tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc ttttagcgcc
    19021 tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat cagatattca
    19081 aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact tttggcaata
    19141 cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa acacaagcgg
    19201 atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg ggaaattctt
    19261 tccgctaata aatgagggtg agccataatg
//
LOCUS       DAJTYI010000072        19180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYI010000072 DAJTYI010000000
VERSION     DAJTYI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            395..583
                     /locus_tag="OO564_002419"
     CDS             395..583
                     /locus_tag="OO564_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6653516.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            534..1229
                     /locus_tag="OO564_002420"
     CDS             534..1229
                     /locus_tag="OO564_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6653517.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(1341..1688)
                     /locus_tag="OO564_002421"
                     /pseudo
     CDS             complement(1341..1688)
                     /locus_tag="OO564_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1657..3314)
                     /gene="dpiB"
                     /locus_tag="OO564_002422"
                     /pseudo
     CDS             complement(1657..3314)
                     /gene="dpiB"
                     /locus_tag="OO564_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            3695..4753
                     /gene="citC"
                     /locus_tag="OO564_002423"
     CDS             3695..4753
                     /gene="citC"
                     /locus_tag="OO564_002423"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6653518.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            4768..5064
                     /gene="citD"
                     /locus_tag="OO564_002424"
     CDS             4768..5064
                     /gene="citD"
                     /locus_tag="OO564_002424"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6653519.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5061..5969
                     /gene="citE"
                     /locus_tag="OO564_002425"
     CDS             5061..5969
                     /gene="citE"
                     /locus_tag="OO564_002425"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6653520.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            5980..7512
                     /gene="citF"
                     /locus_tag="OO564_002426"
     CDS             5980..7512
                     /gene="citF"
                     /locus_tag="OO564_002426"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6653521.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7516..8067
                     /gene="citX"
                     /locus_tag="OO564_002427"
     CDS             7516..8067
                     /gene="citX"
                     /locus_tag="OO564_002427"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6653522.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8042..8920
                     /gene="citG"
                     /locus_tag="OO564_002428"
     CDS             8042..8920
                     /gene="citG"
                     /locus_tag="OO564_002428"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6653523.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            8971..10434
                     /gene="citT"
                     /locus_tag="OO564_002429"
     CDS             8971..10434
                     /gene="citT"
                     /locus_tag="OO564_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6653524.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10548..11354
                     /gene="rna"
                     /locus_tag="OO564_002430"
     CDS             10548..11354
                     /gene="rna"
                     /locus_tag="OO564_002430"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6653525.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            11583..11993
                     /gene="rnk"
                     /locus_tag="OO564_002431"
     CDS             11583..11993
                     /gene="rnk"
                     /locus_tag="OO564_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6653526.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12224..13461)
                     /locus_tag="OO564_002432"
                     /pseudo
     CDS             complement(12224..13461)
                     /locus_tag="OO564_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            13682..14110
                     /gene="uspG"
                     /locus_tag="OO564_002433"
     CDS             13682..14110
                     /gene="uspG"
                     /locus_tag="OO564_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6653527.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14231..15796)
                     /gene="ahpF"
                     /locus_tag="OO564_002434"
     CDS             complement(14231..15796)
                     /gene="ahpF"
                     /locus_tag="OO564_002434"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6653528.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16041..16604)
                     /gene="ahpC"
                     /locus_tag="OO564_002435"
     CDS             complement(16041..16604)
                     /gene="ahpC"
                     /locus_tag="OO564_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6653529.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16976..17722
                     /gene="dsbG"
                     /locus_tag="OO564_002436"
     CDS             16976..17722
                     /gene="dsbG"
                     /locus_tag="OO564_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6653530.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            17932..18834
                     /locus_tag="OO564_002437"
     CDS             17932..18834
                     /locus_tag="OO564_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6653531.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            18981..19180
                     /locus_tag="OO564_002438"
                     /pseudo
     CDS             18981..19180
                     /locus_tag="OO564_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         4818 a         4939 c         4613 g         4810 t
ORIGIN      
        1 ttttttgcac ggcgttacca gccttgttta ccgtttcctg taaaccgagg ggccgtttct
       61 ggcctgcatg gcatgatttc cgcttttaaa cggggaattc agcgtgctga ttggctatgt
      121 aagggtatca acaaatgacc agaatacaga cctgcaacga aacgctcttg tttgtgcagg
      181 atgtgaataa atatttgaag ataaattaag cggaacaagg acagaccgac cgggattaaa
      241 acgtgcttta aagcgccttc aaaaaggaac gcatatagaa aacgacgatc gaatcaatta
      301 aatcgatctg tattaccgat cgattatgtc ccgataacca cacccgactg atgagatttt
      361 ttaagataac ggcgaattga tgcaaggagg tgaaatgaaa tcaattcgct gtaagaactg
      421 caacaaactg ttatttaaag cggattcctt tgatcacatt gaaatcaggt gtccgcgttg
      481 caaacgtcac atcataatgc tgaatgcctg cgagcatccc acggagaaac attgtgggaa
      541 aagagaagaa atcacgcatt ctgacgaaac cgtgcgttat tgagtatgaa ggccagattg
      601 ttggctatgg ttcaaaggag ctgcgcgttg aaaccatatc ctgctggctg gcccgcacaa
      661 tcattcagac aaagcactat tcccgccgtt ttgtgaataa ctcttacctt cacctgggag
      721 tattcagcgg gcgtgatctg gttggcgttc tccagtgggg atatgccctt aaccccaact
      781 caggtcgtcg tgtcgtgctt gaaacggata accggggcta tatggagttg aatcgcatgt
      841 ggctacacga cgacatgccc cgcaactctg aatcacgggc catcagctac gcgctgaaag
      901 ttatcagatt actgtatccg tcagtggagt gggttcagtc ctttgctgac gaacgctgcg
      961 gactcgcagg cgttgtgtat caggcgtcga attttgattt tattggcagc catgaaagca
     1021 cgttctacga gctggatggt gagtggtatc acgagataac gatgaacgcg attaagcgcg
     1081 gagggcaacg aggcgagtat ttgcgagcta ataaggagcg tgccgtggtg cacaaattta
     1141 atcagtatcg ctacatcaga ttcctgaaca agcgagcaag gaagcggcta aataccaaac
     1201 tattcaaggt tcagccatac cctaagtgat ccccatgtaa tgaataaaaa gcggtaatta
     1261 atactgctgt ttcatttgaa gcgcgaaagc taaagttttc gcatttatcg tgaaacgctt
     1321 tcgcgttttt cgtgcgccgc ttcacccagc cgttcatagg caattggctt aatgagataa
     1381 tcaaatacac cacaacgtac ggcttcagac accgtttcca tatcactggc tgcagtggta
     1441 aacaccacgt cgccgggata atgcgcctgc accagttcat gcagtaaatt aatccctcta
     1501 ccgtcaggaa gatagttatc gagcaagatt agccccggct taaaacgctc gatcatcatt
     1561 cgggcctgcg ccaggtttcc cgccagtaat atctgactga atccgggaat gtgacgaata
     1621 tattccgcat gcatctctgc cagcggcgtt tcgtcctcaa cgatcaatag ggttaatgga
     1681 gctgtcatta ggtttcactt tcggaatata gattgaaaat aaggtaccgc agggatcatt
     1741 atcttcgaga gtgataacac caccgcagcg cgttacgtag ctggcaatca agtacaaccc
     1801 aatgccatgt tcaccgggct cgtcagcacg cgtactgacc cctgctcaaa tattttgtct
     1861 cgtagagact ctggaacgcc gcagccctga tcggcgactt caatcaccac atcatcgcct
     1921 tcatcgctga ggaataattc aacgctcttg tttccttcat cgctacgcag gctggcttcg
     1981 aaggcgttat caagtaaatt gccaacaatg gcggcaaact cggtgctatc cagtcctggc
     2041 ggcagttgcg aaagctggct accggggacg atgatcattt ttagccccag ttcgcgggcg
     2101 cgctgcactt taccaaaaag cagccccgcc acctggcgat cggcaaacgc ctcgcgcagg
     2161 ctgtcaataa gctgttgctg ggcctgagac tccccctgca ccatcgccag cacgcgatca
     2221 tactctttca tctgcaacag accattgaga gtcgacatcc aattgagatg ctcgtgtcgc
     2281 aatgtacgaa ggctctcaac gtattgtttt atttgcgtca gttgcgcatt gagggtggaa
     2341 atttcgtctt tactgcgaaa gctgataatg gctcccagca aatcatcacc tgaacgaata
     2401 gcttcccgat tggcaataac gctcagaccg ttaaagttag ccaccacatc ctgacgtttt
     2461 tcatcaatct gttcggtaaa gaaatcggca gacctgacca cttcagcaat gggtttaccc
     2521 aaccattgcc gtccagggga actcagcccc agcatctttc ttgcgttacg attgatggcg
     2581 gtaatgtaac cgtgcggatc caccgcaatc agcccttcat aaaccgaact aaacagcgcc
     2641 tcttgctgac ggaccacccg tgcgatttgc tttggctcca tgcccatcat ctgccgacgg
     2701 atatgcgcgg ccaggaacca cgacagcaac atcagaatcc ctaacagcac gacaaacaca
     2761 cctgccatcg gtaataaaaa ctcagcccgc cagctatcga ttttactcac caggtagcca
     2821 atcgacacca cgccgatgac ttttccatcg tcatcaaaga ttggcgtttt ggcgcgcatc
     2881 gccataccca ttgacccttt cccggtaatg aagtagctct cccctttctc cagcgcgccc
     2941 tgtttggtga actgcatagg ataaccaatt ttctccggat taggatggta aaggcggatc
     3001 gagtgccggt cgccaatcac cacataatca aaatcggtat ctctttgtaa tttgttagcg
     3061 atggtcgcca gccgtttgta gtcacgcgtt ttcaccgcag agatgacact gtcattggag
     3121 gcaataattt tcgcctgatt cattgccatg tcgcgtacat gaagcgttaa atagtcctca
     3181 aaactggccg taaaatattg cgccagggct gcaatgacaa atattgagaa caccagaatc
     3241 agcaaaaaga tacgcagcgg aaatgtcagt ctttggaaaa atgcaaactg tttattctcg
     3301 ttaagctgca acattgtttt ccttgcgggt tgtcccgagc ctctttgacg gatgtaatta
     3361 aaatgttgaa tcaaaataca tagatattaa ttatgatcgt ggcaaatttt attcattaaa
     3421 tcaattaaat cacttaaacc tgcgagtcat ttcgtaatta cattaattaa aaaacttaaa
     3481 accattaaac aaataaaaaa accactaact ctatgtgaaa taaatcaaaa tttcacgccg
     3541 aaatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc accccccttt
     3601 ctcccaagtg aaaataaaag gttatcagtt tgcaacattg aacaacattc gttgcaaatc
     3661 gataacaaca tgcaccttca ggatactatt tattatgttc gtcaatgata ttttcacccg
     3721 cgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
     3781 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
     3841 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
     3901 cggtgaagga ctggcgctga cattagccac tgaactgata aacctcgcct atgagcggca
     3961 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
     4021 tttttccacg ctgacctgcg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
     4081 actgaaacgc tatgccgaat cgctgaaaaa attgcgtcat ccagggaaca agattggctg
     4141 cattgtgatg aacgccaatc cctttacgaa tggtcaccgt tatctgattc aacaagctgc
     4201 ggcacagtgc gactggtttc atctgttttt agtcaaagaa gattcttcac gcttccccta
     4261 tgaagaccgg ctggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
     4321 tcgcggctca gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
     4381 gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc agtacctcgc
     4441 tcccgcgctt ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
     4501 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg cagcgcccat
     4561 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     4621 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     4681 gctgcattat ttgcagaacc tgcttgaaca ctcccgccag gacgcggcag ctcgtcaaaa
     4741 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     4801 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgacctg
     4861 caaatcaata gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     4921 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     4981 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     5041 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     5101 gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac tccttcatct
     5161 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     5221 ccgcccgccg catggtttat cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     5281 ttgtgcgtgt caacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     5341 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     5401 ttgaaaaaga gatcctgcgt atagaaaaag cctgtgttcg tgaacccggc agcaccggcc
     5461 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     5521 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     5581 aacgctcccc ggaaggaact gaactgctgt tcgcacgctg ttccattttg caggccgcgc
     5641 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     5701 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     5761 cgcgtcagat tgatctgctg cacaacctct acgcaccgac ccagaaagaa gtggatcacg
     5821 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     5881 tgaacggtaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctcccgtg
     5941 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     6001 tctcaacgac aagaacgggt agcggcctgg aatcgtcgca ctgaatgcga tcttgccgct
     6061 ttccagaact caccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     6121 gccaacctgg aagaagcgat tcgtcgctca ggtttacagg acggcatgac ggtttccttc
     6181 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     6241 atgggcttta aaaacctgac cctggcgtcc agctccctga gtgattgcca tgcgccgctg
     6301 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctctggcct acgcggtcca
     6361 ctggcggaag agatctcccg tggtctgctg gcggaaccgg tgcagatcca ctctcacggc
     6421 ggtcgtgtgc atctggtaca gagcggcgaa ctgaatatcg acgtggcttt cctcggcgtc
     6481 ccgtcctgtg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     6541 ctcggctatg caatggttga tgccgacaac gcaaaacagg tcgtgatgct taccgaagaa
     6601 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tttgatcgtc
     6661 aaagttgatc gcgttggcga tgctgcaaaa atcggcgctg gtactacccg tatgaccact
     6721 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctctggctac
     6781 ttcaaagaag gtttctccat gcaaaccggc accggcggcg cttcgctggc ggtaacccgt
     6841 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct cggcggtatt
     6901 accgcgacga tggttgatct gcacgaaaaa ggtctgatcc gcaaactgct ggatgtgcag
     6961 agctttgaca gccatgctgc gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     7021 gccaaccagt acgctaactg gggttcgaaa ggcgcatcgg ttgatcgtct cgacgtggtg
     7081 gtactgagcg cgctggaaat tgacaggcag ttcaacgtta acgtgctgac cggttctgac
     7141 ggcgtactgc gtggcgcttc cggtggtcac tgcgataccg cgattgcctc tgcgctttcc
     7201 atcatcgtcg cgccactggt acgcggtcgt attccgactc tggtggataa cgtgctgacc
     7261 tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat cgcagttaac
     7321 ccggcacgtc cggaactagc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     7381 gagtggctgc gcgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     7441 gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag
     7501 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt
     7561 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgcctg gctcaagcac
     7621 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagcgag
     7681 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cacaaagcag
     7741 ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga gggcatgttg
     7801 agcattgcag ccccggctcg cgatctcaag ctcgccacca ttgagcttga acatagtcat
     7861 cctctcgggc ggttatggga tatcgatgtc ctgacgcccg aaggcgaaat tctctcccgc
     7921 cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc
     7981 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac
     8041 gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga
     8101 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     8161 cctcgtggat cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg
     8221 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     8281 agaaatggca ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg
     8341 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     8401 gctgctatgt gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac
     8461 cgtttgttct acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa
     8521 taattcacga ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     8581 cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccttgccgc attacctcac
     8641 tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat
     8701 ggctaccaac ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct
     8761 acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaacccccg ccgatctcga
     8821 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     8881 cgacctgctg atccttacct ggtttttagc acagatttaa ttatttaagc acttgataaa
     8941 tttggaaata ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa
     9001 ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg
     9061 ccgccgcagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc
     9121 gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt
     9181 aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag
     9241 gcgctgaaat ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca
     9301 tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg
     9361 gtgaaattca tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt
     9421 ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc
     9481 attaaaaacc tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt
     9541 ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcatc catgtttgtc
     9601 accggtgcgg caccaaacat gctgggtctg gagttcgtca gcaaaattgc cggtatccag
     9661 attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt
     9721 gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca
     9781 acctgggcgg gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg
     9841 attggccttg tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct
     9901 actgcggttg gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa
     9961 gacattaccc gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg
    10021 atggctaacg gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg
    10081 cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt
    10141 gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg
    10201 gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg
    10261 tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac
    10321 ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt
    10381 tacatctcta tgttactgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt
    10441 cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta
    10501 tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct
    10561 gccgtaacgc cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag
    10621 cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa
    10681 ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc
    10741 aaaccgaaac gaccaacaaa gctgattttc tgaccgtaca tggtctgtgg ccaggattgc
    10801 ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta
    10861 ctcgcccaat cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa
    10921 ccgggttatc actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac
    10981 gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg
    11041 acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa
    11101 aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg
    11161 ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg
    11221 cgtatttgac tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg
    11281 caaactcatt cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata
    11341 aagcgggtta ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc
    11401 gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac
    11461 ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt
    11521 gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa
    11581 aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc
    11641 tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc
    11701 gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg
    11761 ttaaattccg caatcttaga gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa
    11821 aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg
    11881 ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg
    11941 aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc
    12001 tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc
    12061 agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt
    12121 gggaataacc atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat
    12181 tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg
    12241 gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg
    12301 gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg
    12361 cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct
    12421 caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg
    12481 tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc
    12541 ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac
    12601 cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt
    12661 caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa
    12721 ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag
    12781 cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca
    12841 accgtgcaca ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg
    12901 aaccttgttg gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa
    12961 ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt
    13021 tagggacgcg gacatattcc gcctgcccgc taggaacgcc accatacagg tgactataac
    13081 caaacaatgc cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat
    13141 tggtattttc gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa
    13201 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
    13261 caacgacttc cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt
    13321 gcctcgataa agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat
    13381 atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc
    13441 gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta
    13501 agggtagcta tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctggtg
    13561 ttcagtgaga atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat
    13621 cagggataaa tattataatc atggcgaatg atttgttcat gattaacagg gagaaagggt
    13681 tatgtataag gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc
    13741 tgttcgccac gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact
    13801 acccgggtca gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga
    13861 gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga
    13921 tccttcccgc attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt
    13981 ggcagaagaa ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac
    14041 ccatctgtta ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt
    14101 cgttcgctaa cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt
    14161 ttaatgccat aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc
    14221 ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt
    14281 tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg
    14341 ctgcgaacac gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc
    14401 ggttacgttc gactgcgcct tccagccagt tggtgttcgg gagcagacca atctggacaa
    14461 aaataccggc cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac
    14521 caacgacttt gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga
    14581 cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa
    14641 attccagcag cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg
    14701 agttaccgcc gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg
    14761 ggcagtaggt cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc
    14821 attttgcacc cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt
    14881 caatctggtg tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt
    14941 cgatcacatc aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc
    15001 cttcagtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac
    15061 caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg
    15121 ctgcacccgc cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct
    15181 cttctgccgc acgtttttcc gcgccagtat caattttggc aacgatttca gtcaacgtca
    15241 tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc
    15301 gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt
    15361 tcaatacgct catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg
    15421 agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact
    15481 gcgcttcttt cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact
    15541 cgtggcccag cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca
    15601 ggaaagacgg cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca
    15661 gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg
    15721 tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca
    15781 tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg
    15841 cggctacctg gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc
    15901 cgaagaatta ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa
    15961 tccccgggag cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg
    16021 gcgtgaaaga cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt
    16081 gcttcacctt ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga
    16141 gctgctttga ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact
    16201 tcgattgcct ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct
    16261 tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc
    16321 gcatatttga ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga
    16381 gtatcggtag atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca
    16441 gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag
    16501 acgctccagc ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat
    16561 gcctggtttt taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg
    16621 ttttcgatga gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc
    16681 aaatcgataa gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca
    16741 gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt
    16801 tggtatcttc agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca
    16861 tggattacaa caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac
    16921 agtgataggt tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt
    16981 aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc
    17041 cccagtaaaa gcgattgaaa aacatggcat tacaatcatc aaaacgttcg atgcccccgg
    17101 aggaatgaaa ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc
    17161 cgatggtaag cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa
    17221 cacacttatc gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga
    17281 acaatcccac tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttcgc
    17341 cgatccgttc tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc
    17401 tggcaaagtg caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac
    17461 agcagcggca attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc
    17521 tggttgcaag cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt
    17581 aagtgacaat gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta
    17641 catgagtaag gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa
    17701 tatcattatg ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt
    17761 tcaaccatcc taaaaaagca tgaattgctc gctctaccct aagtggctgt gctattttag
    17821 aaaaacttaa tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa
    17881 cattgattaa gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat
    17941 ctctacgact tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag
    18001 catttaagca ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag
    18061 tcgttacaac gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt
    18121 atcgcgccga cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc
    18181 cttgaacaaa caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac
    18241 ggccctcagc tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat
    18301 gcctctgtcg agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg
    18361 ttggttcacc gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc
    18421 atttgcatgc cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga
    18481 attactgaca atagcactta tgaacaaatt atggcagaag agttcaccca attaatatcc
    18541 aaatcagcag gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa
    18601 atttcatttc gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta
    18661 ctgggaatag tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag
    18721 ataaaactag aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct
    18781 ttaaataatt tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata
    18841 actacagaca gctactataa ctttcatcta tttattcaca gctcgaatga aaaataaaac
    18901 actctgcttt acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa
    18961 cgttcagaga gcgtcttcag atgtctattt ataaaattcc tcttccactc aatattctgg
    19021 aagctgcacg agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat
    19081 tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa
    19141 tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc
//
LOCUS       DAJTYI010000073        18929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYI010000073 DAJTYI010000000
VERSION     DAJTYI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..67
                     /locus_tag="OO564_002439"
     CDS             <1..67
                     /locus_tag="OO564_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6653532.1"
                     /translation="FSTVFKQRFGMTPGEYRRKFR"
     gene            complement(239..568)
                     /locus_tag="OO564_002440"
     CDS             complement(239..568)
                     /locus_tag="OO564_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6653533.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(573..758)
                     /locus_tag="OO564_002441"
     CDS             complement(573..758)
                     /locus_tag="OO564_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6653534.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(755..3394)
                     /locus_tag="OO564_002442"
     CDS             complement(755..3394)
                     /locus_tag="OO564_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6653535.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3602..4591
                     /gene="ldhA"
                     /locus_tag="OO564_002443"
     CDS             3602..4591
                     /gene="ldhA"
                     /locus_tag="OO564_002443"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6653536.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4702..5049
                     /gene="hslJ"
                     /locus_tag="OO564_002444"
     CDS             4702..5049
                     /gene="hslJ"
                     /locus_tag="OO564_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6653537.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5046..5312)
                     /locus_tag="OO564_002445"
     CDS             complement(5046..5312)
                     /locus_tag="OO564_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6653538.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5586..9110
                     /gene="nifJ"
                     /locus_tag="OO564_002446"
     CDS             5586..9110
                     /gene="nifJ"
                     /locus_tag="OO564_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6653539.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9477..10606
                     /gene="ompN"
                     /locus_tag="OO564_002447"
                     /pseudo
     CDS             9477..10606
                     /gene="ompN"
                     /locus_tag="OO564_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            10747..11181
                     /gene="uspF"
                     /locus_tag="OO564_002448"
     CDS             10747..11181
                     /gene="uspF"
                     /locus_tag="OO564_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6653540.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11357..12292
                     /gene="ttcA"
                     /locus_tag="OO564_002449"
     CDS             11357..12292
                     /gene="ttcA"
                     /locus_tag="OO564_002449"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6653541.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12421..13788)
                     /gene="dbpA"
                     /locus_tag="OO564_002450"
     CDS             complement(12421..13788)
                     /gene="dbpA"
                     /locus_tag="OO564_002450"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6653542.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14266..15249)
                     /gene="zntB"
                     /locus_tag="OO564_002451"
     CDS             complement(14266..15249)
                     /gene="zntB"
                     /locus_tag="OO564_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6653543.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15504..16736
                     /gene="dgcM"
                     /locus_tag="OO564_002452"
                     /pseudo
     CDS             15504..16736
                     /gene="dgcM"
                     /locus_tag="OO564_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(16757..17320)
                     /gene="smrA"
                     /locus_tag="OO564_002453"
     CDS             complement(16757..17320)
                     /gene="smrA"
                     /locus_tag="OO564_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6653544.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17574..>18929)
                     /locus_tag="OO564_002454"
     CDS             complement(17574..>18929)
                     /locus_tag="OO564_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6653545.1"
                     /translation="IGGGLSAGKDEMAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFT
                     LSAGIFTLMSIACGLAWNIQIMILFRALQGAAGASMIPLVFTTAFIYYQGKELGLAAA
                     VVSALASLSPTLGPTLGGWLTDNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLL
                     KVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTSVLALVSFILFAARTLTIS
                     NPIMDLHAFKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTG
                     IFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGI
                     LQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSR
                     MGEKMVSVPHTVNDFISRSALFFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPF
                     C"
BASE COUNT         4978 a         4811 c         4462 g         4678 t
ORIGIN      
        1 tttctcaacg gtatttaagc aacgctttgg gatgacgcca ggcgagtatc gccgtaaatt
       61 ccgctaacta attggcgttg catgtactgc aacgccaatt agttaaaaca gatatttgac
      121 tatgcatttt tcagggcgaa aggcaatttg acaggagttt ttgaatattc accttgtttc
      181 aggaaaatag atcaatcgct ttctcctgta gcgttatccc gatattgcgg ttttgctatt
      241 aaaactttcg cacccattta ccgtttatcc cttgtacata ttcccccggt ttggcccgag
      301 ctacgagctt ttgcccggcg agtttcgcga tatcatctac agacacatta ttttgctttg
      361 cccgttgttg gtaactcgct ttacgttcgg cattaatttc ggttactaat ttctctgttt
      421 cagcatccgt tttcagcgca accagataac cataaaatgt ttcacctacc cgcccctgag
      481 ttctggcttc atccagagtc aatgccatta cattgctgct taccagccca acaagaaacg
      541 cacaaagaag taatgttttc ttcatcatcg cctcagaaaa gatcgctacg agtttcaagc
      601 agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt aacgttcata
      661 ttgatagtga tcggttcctt aggtgcagcg acttcaatgc gaggcgtaca gccaaccagc
      721 ataaaagatg acgtcaacgc agccagtaaa attttcattg tttttcctca cactcttttc
      781 cttgcggaca gtcatttccc ggcagacgtg cgttctgctc caaccatgcc tggagattat
      841 cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg taattaaggt
      901 ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatgttggcc tgcatggtta
      961 ataaaccgag attatctaag ttaatttttg tcgatgaccg gctaatttcc atatagcgca
     1021 accagttaat tgctgaaccc gcagtcatat tgtctttcac caccgcatct gcggtatctt
     1081 tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc actatccatt
     1141 tttcgttgtt tagccataac ggcaatgcac cactaaatgc cccggacatg gcgaattgtt
     1201 tcggattgac ggcgctaacc agttcgctgg atgagagatt attcagccgc aacagcgccg
     1261 ggtcatgttg cggcatacgt aattgtttca tcagcacgtt accgcctaac acatcgacgc
     1321 taacatcagt caacagcaag ggttcttctt cagtccacgg ataacgccct tgcaaatcag
     1381 ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac gtaacggggc
     1441 cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca aaatcgacac
     1501 cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg tgccctcccg
     1561 cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt tcaccatcgc
     1621 gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgttttg
     1681 gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc
     1741 caatctcacc aacatgtaaa tcgcctttaa agaggaaata ggtcggatcg cgcccatcga
     1801 cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg
     1861 cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg
     1921 gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt aactggtcga
     1981 aaccggtgga gagatcggtc agcgtaatcg tgctgtcatg ccactcacct tttcctgcga
     2041 catcccaggt ggcattcatc ggtgtaaaac tcccttttcc ccagtagcgc cactgccagc
     2101 ggccagcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca
     2161 gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca cgttgggtga
     2221 cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg atgactcttc
     2281 cctttgaacg aagtaacgcg cctggctcaa aggtcagcgt tgggtcagac agacttccac
     2341 ttagctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca
     2401 gctgcagagg cagttgactg ttatccatgc ttaattttcc tgggccaaaa ttaagcaccg
     2461 cgttgccctt acccgcttgc ccctgggtca gcacactcag tcgtccgctg accagagcgt
     2521 tctcaagccc tgcctgccag ttgtcaactt tgacacccag tcggccactc aaaggaaaac
     2581 ctgcatacag ccagctccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc
     2641 acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacgatcaat tgcccgctat
     2701 tttcctgcca gtccagctcg gcatccacca gtgacggttc ctgcggcaag tttaacgtcg
     2761 cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta aattccccca
     2821 ccagtttaac cggcggctga ttttcaaacg cgacgacatc cagttcgctg actgtaagtt
     2881 gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga
     2941 tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga gaaaaaatca
     3001 gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg agggtttttg
     3061 gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa tcaagttcta
     3121 ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg gtgatatgcg
     3181 caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct ttacgcgtaa
     3241 tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg tccgccagcg
     3301 tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata atcagtaata
     3361 acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct gatttcgcga
     3421 aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg aaggttgcgc
     3481 ctacactaag catagttgtt gatgaatttt tcaatatcgc catggctttc aattatattt
     3541 gaaattttgt aaaatatttt tagcagctta aatgtgattc aacatcactg gagaaagtct
     3601 tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa
     3661 cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa
     3721 aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccgcccggt
     3781 gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa
     3841 taacgtcgac cttgatgcgg caaaagaact ggggctgaaa gtagtccgtg ttccagccta
     3901 tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat
     3961 tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggctt
     4021 tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct
     4081 gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcagc
     4141 ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt
     4201 tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg aagccgcctt
     4261 cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc
     4321 tcaggcggca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtcta
     4381 tgagaacgaa cgcgatctgt tctttgaaga taaatccaac gacgtgatcc aggatgacgt
     4441 attccgtcgc ctgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac
     4501 agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa
     4561 aggtgaaacc tgcccgaacg aactggttta atcttgccgc tcccctgcaa tccaggggag
     4621 ctgattcaga taatccccaa tgacctttca ttctctattc ttaaaatagt cctgagtcag
     4681 aaactgtaat tgagaaccac aatgaagaaa gtagccgcgt ttgttgcgct aagcctgctg
     4741 atggcgggat gtgtaagtaa tgacaaaatt gctgttacgc cagaaatcag ctttggtgaa
     4801 aaaatgatga tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat
     4861 ggtgaactga cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt
     4921 aatgaactcg ataacaccat tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc
     4981 gcgaaccagt taacgctggc gaccgcgaaa cagacattaa cttataagct ggcggattta
     5041 atgaattaat agctgccaca gctcccggcg gcaagtgact gttcgctaca gcgtttgccg
     5101 ttgggtaatg cacacatccc aatcgccgta ccatccagtt gacgggcaac agaaagcgaa
     5161 ccgccgatca ttgcacaatt tgcttctcca ctactggaca tcgacgcttt taaacttggc
     5221 gctacgtgcg ccgcagtcgc ctgctgaaca ggctcactac tacacgccga caacaataaa
     5281 gcggcacacc ctacccaaaa cgctgctcgc atctcttctt cctctgatct tcaagccaaa
     5341 cgacaccgcc ataaataata ggcagcacag agggcggcgt cgagagctgt cctgcgcgtt
     5401 gccccgcatt tttacttttt tatggctatt tttttgccct ctgtttgatc aaaacattca
     5461 ttactctgat gtgggggaca caaaagcgaa aatgcagaag aaagccattt gctaaaattg
     5521 aaggattact actgggcgcg cagcaatttc gtgcgcccct catttgcgca atgtaagggt
     5581 gtcatatgat tactattgac ggtaatggcg cggttgcttc ggtggcgttt cgcaccagtg
     5641 aagttatcgc catctaccct attaccccca gttccacgat ggcagaacag gctgatgcct
     5701 gggccggaaa cggcttgaag aacgtttggg gagacacacc acgcgtggtt gaaatgcagt
     5761 cggaagcggg tgctatcgct accgtgcatg gcgctttgca gacgggtgcc ctttcaacat
     5821 cgtttacgtc atcgcagggt ttgctgctga tgatcccgac gctgtacaaa ctggcaggcg
     5881 aactgacgcc atttgtcctg catgtagcgg cacgtaccgt tgccacacat gcactttcta
     5941 tttttggcga tcattccgac gttatggcgg tgcgccagac gggttgcgcg atgttgtgtg
     6001 cggctaacgt ccaggaagcg caagactttg ctctcatttc acacatcgca acgctgaaga
     6061 gccgcgtgcc atttattcat ttctttgatg gttttcgcac gtcccacgaa atcaataaaa
     6121 ttgtcccgct ggccgatgac actattcttg atctcatgcc gcaggccgaa attgatgctc
     6181 atcgcgcccg ggcgctcaac ccggaacatc cggtgatccg cggtacgtcc gccaatcctg
     6241 acacttattt ccagtctcgc gaagccacca acccctggta caacgcggtc tatgaccatg
     6301 ttgaacaggc gatgaatgat ttcgctgccg cgacaggtcg tcagtatcag ccgtttgagt
     6361 attacgggca tccgcaagcg gaacgggtga ttatcctgat gggctctgcc attggcacct
     6421 gtgaagaagt ggttgatgaa ttgctaaccc gtggcgaaaa agttggcgtg ctgaaagttc
     6481 gtctgtaccg tcccttctcc gccaaacact tgctgcaagc tctgccggga tccgtacgca
     6541 gcgtggcggt actggacaga accaaagaac ccggtgccca ggcagaaccg ctctatctgg
     6601 atgtgatgac cgcactggca ggagccttta ataatggtga gcgcgaaacc ctgccccgtg
     6661 tcattggtgg gcgctatggt ctttcatcca aagaatttgg cccggactgt gtactggcgg
     6721 tatttgccga gctcaacgcg gctaaaccta aagcacgctt tacggttggt atttacgatg
     6781 atgtgaccaa tctgtcactg ccgttgccgg aaaacactct gccaaactcg gcgaaacttg
     6841 aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc cgcaaccaaa aacaatatca
     6901 agattatcgg taattccacg ccgtggtacg cacagggcta ttttgtttac gactccaaaa
     6961 aagcgggcgg cctgacggtt tctcaccttc gcgtgagcga acagccgatt cgttccgctt
     7021 atctcatttc ccaggctgat tttgttggct gccaccagtt gcagtttatc gataaatatc
     7081 agatggctga gcgtttaaaa cctagcggca ttttcctgct taatacgccg tacagcgcag
     7141 atgaagtgtg gtcgcgcttg ccgcaggaag ttcaggccgt gttaaaccag aaaaaagcgc
     7201 gcttctatgt gattaacgcg gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta
     7261 ataccgtcat gcagatggct tttttccatc tgacgcaaat tctgcctggc gatagcgccc
     7321 tcgcagaatt gcagggtgcg attgccaaaa gttacagtag caaaggtcag gatctggtgg
     7381 agcgcaactg gcaggctctg gcgctggcgc gtgaatccgt agaagaagtt ccgttgcaac
     7441 cggtaaatcc gcacagcgcc aatcgaccgc cagtggtttc cgatgccgcc cctgatttcg
     7501 tgaaaaccgt aaccgcggcg atgctcgccg ggcttggtga tgccctcccc gtttcggcgc
     7561 tgccgccaga cggcacctgg ccgatgggca ccactcgctg ggaaaaacgc aatatcgccg
     7621 aagagatccc catctggaaa gaggaactct gtacccaatg taaccactgc gtcgccgctt
     7681 gcccacactc agctattcgc gcaaaagtgg taccgcctga agcgatggaa aacgcccctg
     7741 ccagcctgca ttcgctagat gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc
     7801 aggtggcacc ggaagattgc accggttgta acctgtgcgt cgaagtttgc ccggcgaaag
     7861 accgtcagaa tccagagatt aaagccatca atatgatgtc tcgccaggaa catgtcgaag
     7921 aagagaaaat caattacgat ttcttcctca acctgccaga aatcgaccgt agcaaactgg
     7981 aacgtattga tattcgtaca tcgcagctga ttacaccgct gtttgaatat tcaggtgctt
     8041 gccccggttg tggcgagacg ccgtatatta aattgctgac tcagctctat ggtgaccgga
     8101 tgttgatcgc taacgccacc ggctgttctt caatttatgg cggtaacctg ccctctacac
     8161 cgtataccac cgatgccaac ggtcgtgggc cggcatgggc gaactctcta tttgaagata
     8221 atgccgaatt tggccttggt ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc
     8281 gtctgctgga tcaatttgcc gataaaatcc cggcggaatt actgacggcg ttgaaatcag
     8341 acgctacgcc agaggttcgt cgagaacagg tcgcagcttt acgccagcaa ctcaacgatg
     8401 ttgccgaagc acatgaactg ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc
     8461 tgattggtgg cgacggctgg gcttacgata tcggctttgg cggtctggat catgtattga
     8521 gtttgacgga aaacgtcaac attctggtgc tggatacgca atgctattcc aacaccggtg
     8581 gtcaggcgtc gaaagcgaca ccgctgggtg cagtaactaa gttcggcgag cacggcaaac
     8641 gtaaagcgcg taaagatctt ggcgtcagta tgatgatgta cggtcatgtt tatgtggcgc
     8701 agatttctct cggcgcgcag ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt
     8761 atccgggacc atcgctgatc attgcttata gcccgtgtga agagcatggt tacgatctgg
     8821 ctctcagcca cgaccagatg cgccaactca ctgctaccgg cttctggccg ctatatcgct
     8881 ttgatccgcg tcgtgccgat gaaggcaaac tgccgctggc gctggattca cgcccgccgt
     8941 cagaagcact ggaagaaacg ttacttcacg agcagcgttt ccgtcgtctg aattcgcagc
     9001 agccagaagt ggcagaacag ttatggaaag atgctgcagc tgatttgcaa aaacgctatg
     9061 acttcctagc acaaatggcc ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga
     9121 taaggattat ccaattctaa aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9181 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9241 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9301 tattatcttc gatacattca ccagacttat tatcatttca gaggaattat ttgattaagg
     9361 ttttacttaa ggcgtaacaa atgatattta tcggcaaact gaacttctcc tttaggatgt
     9421 tttcactccc cgaaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9481 aaagaaaagt actggcactt ttaatccctg ccctgctcgc cgcaggtgct gcacatgcag
     9541 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9601 attatttttc tgataattca gcgaaagatg gcgaccagag ctatgcgcgt ctgggtttta
     9661 aaggcgaaac ccaaattaac gatcaactta ctggctacgg tcaatgggaa tacaatattc
     9721 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccggac
     9781 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
     9841 tcgaaggctg gaccgatatg ctgcctaaat ttggcggtga ctcttatacc aatgcagaca
     9901 actttatgac tggtcgagcc aatggcgtcg cgacttatcg taatactgat ttcttcggtc
     9961 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaagga gccagtaatg
    10021 gtcaggaagg caccaataac gaacgcgatg ttcgtcatga aaacggtgac ggctggggtc
    10081 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcttctg
    10141 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10201 ctgctgggct aaaatacgat gctaacaata tttacctagc aaccatgtat tcagaaacgc
    10261 gtaatatgac cccgtttggc gacagcgatt atgcagttgc gaacaaaacc cagaactttg
    10321 aagttactag taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa
    10381 aggccgtgac cttcacgctg ctggtggtgc agacaacccg gcaggtgttg atgataaaga
    10441 tctggttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccaccta
    10501 tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaacggcat
    10561 ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc
    10621 tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac
    10681 tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga
    10741 ggttttatga acagaacgat tcttgtccct atcgatattt cagattcaga attaactcaa
    10801 cgcgtgatta gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg
    10861 acggtaatac cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta
    10921 ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa
    10981 tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc
    11041 attctggaat tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg
    11101 gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc
    11161 tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat
    11221 ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac agtttttata
    11281 tcagatttct tatgctgagc gttccggcat gacgcatact cttctgatgc catataacga
    11341 attgagtcgc ttttaaatgt cgcaaaatca agaaattagt aagaaagaac aatacaacct
    11401 gaacaaatta caaaaacgtc tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat
    11461 gattgaagaa ggcgatcgca tcatggtttg cctctccggg ggtaaagaca gctataccat
    11521 gctggagatt ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt
    11581 taacctcgat caaaagcaac cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa
    11641 gctgggcgtt gagtacaaga ttgttgaaga gaacacttac ggtatcgtga aagagaagat
    11701 tccagagggc aaaaccactt gctcactgtg ttctcggcta cgtcgcggta tcctttatcg
    11761 caccgcaacg gaactgggga cgacgaagat cgcgttgggt caccatcgtg acgatatcct
    11821 gcaaacgttg ttcttaaata tgttctacgg cggcaagatg aaaggtatgc ctccgaaact
    11881 gatgagcgat gatggcaaac atatcgttat tcgtccactg gcctactgcc gcgagaaaga
    11941 tattcagcga tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc
    12001 acagcctaac ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg ataaacgtta
    12061 tccagggcgt atcgagacga tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg
    12121 cgataccaac ctgttcgatt tcaaaggcat tacccacggt tctgaagtgg ttaacggggg
    12181 tgatctggcg tttgatcgcg aagagatccc actacaaccg gcgggctggc agccagaaga
    12241 agatgaaaat cagttggatg agttacggct gaatgtggtt gaagtgaaat aaccaggata
    12301 gcgcccgatg cgcaagcgta tcgggctact cttatggagg ccggataaga cgcggccagc
    12361 gtcgcatccg gcaatcccga ataagatgtt tactcttgca cccggcaatt caacatttca
    12421 ttattttaat aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct gtaactgttt
    12481 ccatgcttta tgagcaacag cctgacggac cgcgacatag acatgcgccg gatgcacggc
    12541 gattttgcca atatctgcgc catcaagccc gatatctcct gtcagtgcac ctaatacatc
    12601 acccgggcgc attttggctt ttttcccgcc atcgatacac aacgttgcca tttctgcttc
    12661 cagcgtcaca atggaactat tagctggcgg cgtttgccag ttaagtttta tctgcaacat
    12721 gtcagaaatg atattggccc gctgtgcttc ttccggagca cagaaactga tcgccagacc
    12781 gctatttcct gcacgagctg tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc
    12841 cagctcaaag ttcaccacca gctcaagcga tttaatatcc agaccacgcg cagcaacatc
    12901 agtcgcaacc agtacacggg cgctaccgtt agcaaaacgt accagggtct gatcgcgttg
    12961 ctccagatcg ccgtgtaacg acaatgcact ttgctctact tcattcagcg cgtcgcagac
    13021 agcctggcaa tcttttttgg tattgcaaaa caccacacaa gaggatggct gatgcaagct
    13081 taataaccgt tgcaacagag gaattttgcc tttactggat gtctcataaa attgttgttc
    13141 aatgggtggc aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg
    13201 tccgctgatt gcggcgatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga
    13261 tgcaggcgca aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg
    13321 gtcggcctca tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa
    13381 gtgatccagc aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc
    13441 acgctgcata ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa
    13501 acgcgccagc cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag
    13561 cactaaagcc agggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa
    13621 agccgccgtt ttgccgctgc cggttttcgc ctgcacgcga acatcttttc ctgcaaggat
    13681 cgccggaagc gcggcggcct gcaccggcgt catggttaaa taacccaact cattaaggtt
    13741 cgtgagttgg gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg
    13801 tggtcatcga cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac
    13861 aatttaatcg gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga
    13921 tcgggcactg gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata
    13981 agtgtcgtca tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa
    14041 taacaggcaa aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg
    14101 cagtaattca aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc
    14161 attcacctgc aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa
    14221 ctttgcggcg tttttgtata cagacagccg aaaaaattgc ttttgttaca accatttact
    14281 acggtgcaac cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga
    14341 aaatccgaat tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt
    14401 cagaaaggta ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc
    14461 taaattttcc tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca
    14521 ggcgtcgatt tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg
    14581 ttggtcatcg ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg
    14641 cggtgccata tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg
    14701 cggtggaatt tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg
    14761 ctcgataaat tcactggaat gatcggtcaa cgcatcgcac acatccacca gccatccccc
    14821 gcaatcggtc ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac
    14881 tttgcgttgt cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag
    14941 ttgatcgggg cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc
    15001 accgaaacgg ctgactcggg gccgtgtgct ctcgcccgcc agcgcatcac gtacgttatt
    15061 gggaagcagc ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg
    15121 gagccagcag ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc
    15181 tctaccatcc agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat
    15241 cgcttccaca gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt
    15301 tacgcaacat ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga
    15361 atacaaaaag caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat
    15421 cattatcaga attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa
    15481 ttgataaaaa taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca
    15541 ccagtcctgt ctggattgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg
    15601 cgcgactgtt gatgcaagac ctcacgttta gtcagctacg aaccggaccc tattccgtct
    15661 cctcacaaaa agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg
    15721 aaatcctcac tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg cttgttttgc
    15781 gagagctgac agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc
    15841 tgggtttaaa agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc
    15901 gcttttttct gactaactct gcaccaatgt tgttgattga cccgtcacga gatggacaaa
    15961 tcgtcgatgc taacctcgcc gcgctcaatt tctatggtta taaccatgaa acgatgtgcc
    16021 agaaacatac ctaggaaata aatatgcttg ggcgtcgcgt catgcctatc atgcatgaaa
    16081 tttcgcattt acccggcggt cataaccctt tgaattttgt tcataaactg gcggatggtt
    16141 ctactcgtca tgtgcagacc tatgccggac cgattgaaat ttatggcgac aagctcatgt
    16201 tatgtattgt gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg
    16261 ctcaccatga cgcgatgacc ggattactga atcggcgaca gttttatcac attacggaac
    16321 caggccaaat gcagcatctc gccatcgctc aggattacag cttgttgctc atcgacaccg
    16381 atcgttttaa acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg
    16441 ccctcgcccg ccccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag
    16501 gcgaagagtt tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg
    16561 aaactatccg cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttaccgtca
    16621 gcattggtgt ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg
    16681 atgatgcttt gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg
    16741 cggatgcgtc tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg
    16801 cctgcgccgt tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg
    16861 cggtgcaata tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga
    16921 caatattggc atgtgattta tcttcccgcc ctttaccatg aataatcagc acattacgca
    16981 aaccatccgc cagggcttgt tgaatgaaac tgaacaccat tttgcggcac tcttccaccg
    17041 gctggcgcaa aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca
    17101 gcaccccatg ctgcaaccct tcctgccgaa actccagcgg ctgacttagc gggatgatgt
    17161 cgagaaatcc ggtagtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac
    17221 gttggttacg cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt
    17281 ccatggcgtc aagaaacagc gatttgtcgt caaggttcat gtaacctcct atgcaccttc
    17341 acacgtcgtg attcagacgg gttaattgtt catacttact gcatcgttcc cttcacagat
    17401 tgaaacaata ctctacttca tacctgtgag tatctgcgca ggcggggcat tttatactag
    17461 cctgttagca gtatcgaaca gtttagcccg agtttaattc agcacaggct ccgatcagga
    17521 tgatttattc atagcaggaa ccaacaaaaa tgcgataaat aacagaccac tgattagcag
    17581 aaaggtaccg aaaatgccat agtttgcgcc tccctcagca tcaattgact caataatttg
    17641 gttgaagcca gtatttccga tgtctggtca cttctactgc gattaaaaaa taaagcactt
    17701 ctggaaatga agtcattcac cgtatgggga acagaaacca ttttttcacc cattctcgaa
    17761 aaatgaaaat tagttcgatt attgagaata gagccacaca gggcaatacc aatcgcgccc
    17821 ccaagattac gtgttaaatt aaatacacca gaggctaact tcagccgttc ttttggaatt
    17881 ccccccagcg ttaatgttac gatgggggcc atcgcaaact gctgcaaaat ccctctaatt
    17941 gcctgtggaa acagtaattc ctgccatccc cattcgtggg ttataggggt aaaaagatac
    18001 attgaaaaca caaatccgcc cagccccgcc atcaacagcc atcgcagatt tattttttta
    18061 cttaaccaga agtaaaaggg aacagaaaat aactggaaaa tacctgttgt gcaaacggca
    18121 aaacctatct cttcggcatt taatccgcga atctgcccca aaaacacagg tattaaatat
    18181 acagtagaga atatcccaac ccctccagaa aaagaaaaaa aacatcccag agtgaaattt
    18241 ttatccttaa aagcatgaag atccattatc ggattggata ttgtaagtgt acgcgcagca
    18301 aataaaataa aactcaccaa tgccagaact gatgtcagca aaatagtatt atcatccagc
    18361 catccccagc gcgctccttc ttcaagggta tattccagac atcccagagt catggccaac
    18421 aggataatac tcggataatc agcaactttc agtaaagaga gatcaggttt atcaaaatta
    18481 accagaaaag gaatactcaa aactaaatat atgccaggta atatattgat ataaaatagc
    18541 catcgccagt cgaggttatc agtgagccag ccccccagag ttggccctag cgtcggtgac
    18601 agtgaggcca gcgcactgac taccgcagca gccaacccca actctttacc ctggtaataa
    18661 ataaacgccg tcgtgaaaac taaagggatc atggatgcgc cagcagctcc ctgtaaagca
    18721 cgaaacaaaa tcatgatctg aatattccat gcaagtccac acgcgatact cattaaagta
    18781 aatattcccg cggatagagt aaaaagccaa cgagtggaaa atacccgtga cagccaccca
    18841 gagagcggaa taacaataat ctcagcaatt aaatagctgg tctgaagcca ggccatttcg
    18901 tctttacctg cggacagacc accacctat
//
LOCUS       DAJTYI010000074        18826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYI010000074 DAJTYI010000000
VERSION     DAJTYI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1960
                     /gene="scpA"
                     /locus_tag="OO564_002455"
     CDS             <1..1960
                     /gene="scpA"
                     /locus_tag="OO564_002455"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6653546.1"
                     /translation="PRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAF
                     DLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAVLPVL
                     AFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMP
                     RFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLFFGIG
                     MDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNVIRTT
                     IEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPLAGSY
                     YIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGKRVIV
                     GVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAAQHNE
                     NLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASEFDAI
                     VAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTPEEIA
                     RLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAFLQER
                     GVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            1953..2948
                     /gene="meaB"
                     /locus_tag="OO564_002456"
     CDS             1953..2948
                     /gene="meaB"
                     /locus_tag="OO564_002456"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6653547.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            2959..3744
                     /gene="scpB"
                     /locus_tag="OO564_002457"
     CDS             2959..3744
                     /gene="scpB"
                     /locus_tag="OO564_002457"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6653548.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            3768..5246
                     /gene="scpC"
                     /locus_tag="OO564_002458"
     CDS             3768..5246
                     /gene="scpC"
                     /locus_tag="OO564_002458"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6653549.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5188..6138)
                     /locus_tag="OO564_002459"
                     /pseudo
     CDS             complement(5188..6138)
                     /locus_tag="OO564_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6305..7045)
                     /locus_tag="OO564_002460"
     CDS             complement(6305..7045)
                     /locus_tag="OO564_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6653550.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(7138..7773)
                     /gene="argO"
                     /locus_tag="OO564_002461"
     CDS             complement(7138..7773)
                     /gene="argO"
                     /locus_tag="OO564_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6653551.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(7912..8772)
                     /gene="mscS"
                     /locus_tag="OO564_002462"
     CDS             complement(7912..8772)
                     /gene="mscS"
                     /locus_tag="OO564_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6653552.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(8963..10042)
                     /gene="fbaA"
                     /locus_tag="OO564_002463"
     CDS             complement(8963..10042)
                     /gene="fbaA"
                     /locus_tag="OO564_002463"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6653553.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(10257..11420)
                     /gene="pgk"
                     /locus_tag="OO564_002464"
     CDS             complement(10257..11420)
                     /gene="pgk"
                     /locus_tag="OO564_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6653554.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(11470..12489)
                     /gene="epd"
                     /locus_tag="OO564_002465"
     CDS             complement(11470..12489)
                     /gene="epd"
                     /locus_tag="OO564_002465"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6653555.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            12861..13292
                     /locus_tag="OO564_002466"
     CDS             12861..13292
                     /locus_tag="OO564_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6653556.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            13315..13893
                     /locus_tag="OO564_002467"
     CDS             13315..13893
                     /locus_tag="OO564_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6653557.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            13894..14202
                     /locus_tag="OO564_002468"
     CDS             13894..14202
                     /locus_tag="OO564_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653558.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            14218..14601
                     /locus_tag="OO564_002469"
     CDS             14218..14601
                     /locus_tag="OO564_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6653559.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            14589..15266
                     /locus_tag="OO564_002470"
     CDS             14589..15266
                     /locus_tag="OO564_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6653560.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            15260..15937
                     /locus_tag="OO564_002471"
     CDS             15260..15937
                     /locus_tag="OO564_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6653561.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(15909..16622)
                     /locus_tag="OO564_002472"
     CDS             complement(15909..16622)
                     /locus_tag="OO564_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6653562.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(16619..17128)
                     /gene="fumE"
                     /locus_tag="OO564_002473"
     CDS             complement(16619..17128)
                     /gene="fumE"
                     /locus_tag="OO564_002473"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6653563.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(17150..18115)
                     /gene="yggF"
                     /locus_tag="OO564_002474"
     CDS             complement(17150..18115)
                     /gene="yggF"
                     /locus_tag="OO564_002474"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6653564.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(18112..>18826)
                     /locus_tag="OO564_002475"
     CDS             complement(18112..>18826)
                     /locus_tag="OO564_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6653565.1"
                     /translation="IGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGI
                     ELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGL
                     TDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDA
                     VPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNE
                     QAEKYLLAQFGVDIGEAAQ"
BASE COUNT         4510 a         5071 c         4724 g         4521 t
ORIGIN      
        1 cccgcgtgcc actatgtata ccgcccaacc gtggaccatc cgtcagtatg ctggtttttc
       61 aacagcaaaa gagtccaacg ctttttatcg ccgtaacctg gccgccgggc aaaaaggtct
      121 ttccgttgcg tttgaccttg ccacccaccg tggctacgac tccgataacc cacgcgtggc
      181 gggtgacgtc ggcaaagcgg gcgtcgctat cgacaccgtg gaagatatga aagtcctgtt
      241 cgaccagatc ccgctgggta aaatgtcggt ttcgatgacc atgaatggcg cagtgctacc
      301 agtactggcg ttttatatcg tcgccgcaga agagcaaggt gttacacctg ataaactgac
      361 cggcaccatt caaaacgata ttctcaaaga gtacctctgc cgcaacacct atatttaccc
      421 accaaaaccg tcaatgcgca ttatcgccga catcatcgcc tggtgttccg gcaacatgcc
      481 gcgatttaat accatcagta tcagcggtta ccacatgggg gaagcgggtg ccaactgcgt
      541 gcagcaggta gcatttacgc tcgccgatgg gattgagtac atcaaagcag caatctctgc
      601 cggactgaaa atggatgact tcgctcctcg cctgtcgtta ttcttcggta tcggcatgga
      661 tctgtttatg aacgtcgcca tgttgcgtgc ggcacgttat ttatggagcg aagcggtcag
      721 tggatttggc gcacaggatc cgaaatcact ggcgctgcgt acccactgcc agacctcagg
      781 ctggagcctg actgaacagg atccgtataa caacgttatc cgcaccacca ttgaagcact
      841 ggctgcgacg ctgggcggta ctcagtcact gcataccaac gcctttgacg aagcgcttgg
      901 tttgcctacc gatttctcag cacgcattgc ccgcaacacc cagatcatca tccaggaaga
      961 atcagaactc tgccacaccg tcgatccact ggccggatcc tattacattg agtcgctgac
     1021 cgatcaaatc gtcaaacaag ccagagctat tatccaacag atcgacgaag ccggtggcat
     1081 ggcgaaagcg atcgaagcag gtctgccaaa acgaatgacc gaagaggcct cagcgcgcga
     1141 acagtcgctg atcgaccagg gcaagcgtgt catcgttggt gtcaacaagt acaaactgga
     1201 tcacgaagac gaaaccgatg tacttgagat cgacaacgtg atggtgcgta acgagcaaat
     1261 tgcttcgctg gaacgcattc gcgccgcccg tgatgatgcc gccgtaaccg ccgcgttgaa
     1321 cgccctgact cacgccgcac agcataacga aaacctgctg gctgccgctg ttaatgccgc
     1381 tcgcgttcgc gccaccctgg gtgaaatttc cgatgcgctg gaagtcgctt tcgaccgtta
     1441 tctggtgcca agccagtgtg ttaccggcgt gattgcgcaa agctatcatc agtctgagaa
     1501 atcggcctcc gagttcgatg ccattgttgc gcaaacggag cagttccttg ccgacaatgg
     1561 tcgtcgcccg cgcattctga tcgctaagat gggccaggat ggacacgatc gcggcgcgaa
     1621 agtgatcgcc agcgcctatt ccgatctcgg tttcgacgta gatttaagcc cgatgttctc
     1681 tacacctgaa gagatcgccc gcctggccgt agaaaacgac gttcacgtag cgggcgcatc
     1741 ctcactggct gccggtcata aaacgctgat cccggaactg gtcgaagcgc tgaaaaaatg
     1801 gggacgcgaa gatatctgcg tggtcgcggg tggcgtcatt ccgccgcagg attacgcctt
     1861 cctgcaagag cgcggcgtgg cggcgattta tggtccaggt acacctatgc tcgacagtgt
     1921 gcgcgacgta ctgaatctga taagccagca tcatgattaa tgaagccacg ctggcagaaa
     1981 gtattcgccg cttacgtcag ggtgagcgtg ccacgctcgc ccaagccatg acgctggtgg
     2041 aaagccgtca cccacgtcat caggcgctaa gtacacagct gcttgacgct attatgccgt
     2101 tctgtggtaa cgccctgcga ctgggcgtta ccggcacacc cggcgcgggg aaaagtacct
     2161 ttcttgaagc ctttggcatg ttgttgattc gtgagggatt aaaggttgct gttattgcgg
     2221 tcgatcccag cagcccggtc actggcggta gcattctcgg ggataaaacc cgcatgaatg
     2281 acctggcgcg tgccgaggcg gcgtttattc gcccggtacc ctcctccggt catctgggcg
     2341 gtgccagtca gcgagcacgg gaattaatgc tgttatgcga agcggcgggt tatgacgtag
     2401 tgattgtcga aacggttggc gtcgcgcagt cggaaacgga agtcgcccgc atggtggact
     2461 gttttatctc gttgcaaatt gccggtggcg gcgatgatct gcagggcatt aaaaaagggc
     2521 tgatggaagt ggctgatctg ctcgttatca ataaagacga tggcgataac cataccaatg
     2581 tcgccattgc ccggcatatg tacgagagtg ccctgcatat tctgcgacgt aaatacgacg
     2641 aatggcagcc acggggtctg acttgtagcg cactggaaaa acgtggaatc gatgagatct
     2701 ggcacgccat catcgacttc aaaaccgcgc taactgccag tggtcgttta caacaagtgc
     2761 ggcaacaaca atcggtggaa tggctgcgta agcagaccga agaagaagta ctgaatcacc
     2821 tgttcgcgaa tgaagatttc gatcgctatt accgccagac gcttttagcg gtcaaaaaca
     2881 atacgctctc accgcgcacc ggcctgcggc agctcagtga atttatccag acgcaatatt
     2941 ttgattaaag gaatttttat gtcttatcag tatgtgaacg ttgccactat caacaaagtg
     3001 gcggtcattg agtttaacta tggacgaaaa cttaatgcct taagtaaagt ctttattgat
     3061 gatcttatgc aggcgttaag cgatctcaac cggccggaaa ttcgctgtat cattttgcgg
     3121 gcgccaagtg gatccaaagt cttctccgca ggtcacgata ttcacgaact gccgtctggc
     3181 ggtcgcgatc cgctctccta tgatgatcca ttgcgtcaaa tcacccgcat gatccaaaaa
     3241 ttcccgaaac cgatcatttc gatggtggaa ggtagtgttt ggggtggcgc atttgaaatg
     3301 atcatgagtt ccgatctgat catcgccgcc agtacctcaa ccttctcaat gacgcctgta
     3361 aacctcggcg tcccgtataa cctggtcggc attcacaacc tgacccgcga cgcgggcttc
     3421 cacattgtca aagagctgat ttttaccgct tcgccaatca ccgcccagcg cgcgctggct
     3481 gtcggcatcc tcaaccatgt tgtggaagtg gaagaactgg aagatttcac cttacaaatg
     3541 gcgcaccaca tctctgagaa agcgccgtta gccattgccg ttatcaaaga agagctgcgt
     3601 gtactgggcg aagcacacac catgaactcc gatgaatttg aacgtattca ggggatgcgc
     3661 cgcgcggttt atgacagcga agattaccag gaagggatga acgctttcct cgaaaaacgt
     3721 aaacctaatt tcgttggtca ttaatccctg cgaacgaagg agtaaaaatg gaaactcagt
     3781 ggacaaggat gaccgccgat gaagcggcag aaattatcca gcataacgac atggtggcat
     3841 ttagcggctt taccccagcg ggttcgccga aagccctacc cgccgcgatt gcccgcagag
     3901 ctaacgaaca gcatgaggcc aaaaagccgt atcaaattcg cctgctgacg ggggcgtcaa
     3961 tcagcgccgc cgctgacgat gtactttctg acgccgatgc tgtttcctgg cgtgcgccat
     4021 atcaaacatc gtccagttta cgtaaaaaga tcaatcaggg cgcggtgagt ttcgttgacc
     4081 tacatttgag cgaagtggcg caaatggtca attacggttt cttcggcgac attgatgttg
     4141 ccgtcattga agcatcggca ctggcaccgg atggtcgagt ctggttaacc agcgggatcg
     4201 gtaatgcgcc gacctggctg ctgcgagcga agaaagtgat cattgaactt aatcactatc
     4261 acgatccgcg cgttgcagaa ctggcagata ttgtgattcc tggcgcgcca ccgcggcgca
     4321 atagcgtgtc gatcttccat gcaatggatc gcgtcggtac ccgctatgtg caaatcgatc
     4381 cgaaaaagat tgtcgccgtc gtggaaacca acttgcccga cgccggtaat atgctggata
     4441 agcaaaatcc catgtgccag cagattgccg ataacgtggt cacgttctta ttgcaggaaa
     4501 tggcgcatgg gcgtattccg ccggaatttc tgccgctgca aagtggcgtg ggcaatatca
     4561 ataatgcggt aatggcgcgt ctgggggaaa acccggaaat tcctccgttc atgatgtatt
     4621 cggaagtgct acaggaatcg gtggtgcatt tactggaaac cggcaaaatc agcggggcca
     4681 gcgcctccag cctgacaatc tcggccgatt ccctgcgcaa gatttacgac aatatggatt
     4741 actttgccag ccgcattgtg ttgcgtccgc aggagatttc caataacccg gaaatcatcc
     4801 gtcgtctggg cgtcatcgct ctgaacgtcg gcctggagtt tgatatttac gggcatgcaa
     4861 attcaacgca cgtagccggg gtcgatctga tgaacggcat cggcggcagc ggtgattttg
     4921 aacgcaacgc gtatctgtcg atctttatgg ctccgtcgat tgctaaagaa ggcaagatct
     4981 caaccgtcgt gccaatgtgc agccatgttg atcacagcga acacagcgtc aaagtgatca
     5041 tcaccgaaca agggatcgcc gatctgcgcg gtctttcccc gcttcaacgc gcccgcacta
     5101 tcattgataa ttgtgcacat cctatgtatc gggattatct gcatcgctat ctggaaaatg
     5161 cgcccggcgg acatattcac cacgatctta gccacgtctt cgacttacac cgtaatttaa
     5221 ttgcaaccgg ctcgatgctg ggttaatggt ttgtcccgtc acgatcttca gcaatatgct
     5281 gaagatcgtg gcggtataac gatgatttct gattgaataa agatattcct gcatatacat
     5341 cggcctgtgc ggcgcatcaa aatatttcag cgttgccgga ttcaccagtc accaggcaaa
     5401 atgcgggatc accgtcagaa aatgtccgcg ctccacggcg ctgattttgg ccataaaact
     5461 gtagggacga taaataatgg tgggattgat cccacaaggt cgcatattcg catcaatcat
     5521 tgcttcgaag ttagcccggt tttgaaagcg catttgtagc caggggagct cctgaagcag
     5581 ctcttgctct tgacgctctt tataacggcg agaaacgaga aagcccaatc acagcggtgg
     5641 taattcactg atagtcaggt tttccagttc ctgaactcgt gcagatacat gctgtgggga
     5701 gataataaaa tctaactgac ggtcaaaaag attatcgata acgccgtttt cgctgaactc
     5761 aactggctgg gcggtcacgc cttcatactt atctccgaga ctaatcaact gatcgaaaat
     5821 aattgtcgga taagtattat ctatgccaat cacaatttct cgtgaacgac gccctgagtt
     5881 atggatttca ttatcaatcg cagaaagtcg ttgataaacc gggaacagtt tttgatacag
     5941 ttcctgaccg gctttattga ggctgatatt attatctttg cgggtaaata gcgtatagcc
     6001 aatttgttct tccagcgcgg caatgctttt accaaatggc gaagccgtca tatggatttt
     6061 ttctgccgcc cgggcaatat tattggtttg cgctaataaa atgaaattgc gcatcttttt
     6121 cgaaataaaa atgtccatcc cccctccccg catccttgtc cgcttaaccg tttcagtcag
     6181 cctatccttg atgaaaccgc gagcaaagat aggtgattac gtcatggttt tacagaaaat
     6241 tacagaaaaa ggaggcaata tcgggtaaag gcattagccc gacgaatacg tcgggctaca
     6301 agtattattg tgctgcaggt gttttagcgg gttgttgatc cacaggttct aactggaaga
     6361 ccacatcgac ctgatcatca aactgaatag cggcctgctc gtaagtttcc tgggcagaca
     6421 ccggcgcggc atcggctttc atcatccgca ccattgggct gggctgatag ttggaaacat
     6481 ggtagcgcac gctatatacc ggccccagtt tacgatgaaa gccgttcgcc agttcctgcg
     6541 cctgatgaat cgcgttatca atcgctgcct tacgcgcttt gtctttatag gcatccggct
     6601 gcgccacgcc cagcgacaca gaacgaattt cgttaagacc cgccttcagc gcgccatcca
     6661 gcaaggaatt cagtttgtct aactgacgga gcgtgacttc caccgttctc acagctcggt
     6721 agcctttcag gatactttta ccatcctgat agtcataatc tggctgggtg cgtaaattcg
     6781 ctgagctgat atcttttttc gcgatctgat tgagttcaag gaaggaaatg tattgtgcga
     6841 cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt cgcggccacg ttaacttcaa
     6901 tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc cgtaccggag gtgacaatat
     6961 gcggtccatc cggcaattcg ttagcctgcg ctgccatccc gctaataccc attaatgccg
     7021 ccagggcgat aactttgaac ttcacgttag tctctccgtg ttaattgact tgcccaaaat
     7081 tcaggacctc tggaggcaag cttagcgcct ctgttttatt tttccatcag atagcgccta
     7141 actgaacaag gcttgtgcat gagcaatacc gtctctcgcc agctgcaagg ctataaacca
     7201 catgacacat cccactacca gattgataat gtgctgtgat tttgccgtgc gcaggcgcgg
     7261 tgccagccag gctgcgagaa tagccagacc aaagaaccac aggaaagagg cgctaattgt
     7321 cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc ccgccaaggc tgcccagtac
     7381 aacaaaagta tccaggtaaa catgcggatt cagccaggtc actgccaaca tggtggcgat
     7441 aattttccat ctgccttgct tcaggacttc ggcactagct aactcaatat tactactcat
     7501 cgctgtttta aaagcgccaa aaccatacca gagcaagaag actacgccgc cccaagtgac
     7561 cagcgctagc aaccacggcg actgcatcaa taacgcgcta ccaccaaaaa tccccgcgca
     7621 aatcaggacc aaatcgctga tagcgcaaag taaggcaatc ataatgtggt actgacgacg
     7681 aatgccctga ttcatcacaa aagcattttg tgggccgagc ggcagaatca tagctgcccc
     7741 aagagcaaga ccttgaaagt aataagaaaa cacgttggat attccgaatt aagtatctga
     7801 tgcagagact atatcgcggg attgttatta gcggaaattg ataatattaa ttggcaatca
     7861 gaataactaa taagagagag gccagagagt attatctggc ctcagcgttg attacgcagc
     7921 tttgtcttct ttcacccgct taaagttcac atccatttgc gggtacggga agctgatacc
     7981 ggcggcatca aattcacgtt taatacgctc cagcacatcc cagtacacgt tttgcagatc
     8041 gccgctgttg ctccagacgc ggaccacgaa attaatcgac gatgcaccaa gttcgttcag
     8101 gcgcacagtc atttcgcgat ctttcaaaat gcgatcttca gactggataa tattggtcag
     8161 gatctgctta acctgatcga tatcggaatc atacgccacg ccaataataa attcgttacg
     8221 gcgaactggc tcgcgggaga agttaataat atttccggca ataattttac cgttcggaat
     8281 aacgataatt ttaccgtctg cagtacgcat ggtggtggag aaaatctgca cactcagcac
     8341 agtaccggct acgccgccga ggtcaacata ttctccggca cggaacgggc ggaacatgac
     8401 aagtaacacg ccagcggcca ggttagaaag tgacccctgc aaagccagac caacagctaa
     8461 gcctgcggca ccgagtacag caatgactga cgctgtttgt acacccacgc gtcccagggc
     8521 agcgattagc gtaaaggcga taataccgta acggactaat gcagaaagaa aatcagcaac
     8581 agtggcatcg attttacggg agatcatcag gcgattcacc gcgttggaaa tcatccgcgc
     8641 gataatcaaa ccaacgatga tgatcgcgag tgccgccacg atgtttactg cataacttag
     8701 cagcagcgcc tggttagcta ccagccagct tcccgcgccg tttatgctat cgacaacatt
     8761 caaatcttcc attcaatatt ccttttcaac tgactcaaaa tggagaaata cactccgccg
     8821 ccttatgacg ggcagtctga caagggtaaa caataaagca taattttgcc aaatagatca
     8881 cagatttctc atctgaatca ccttgctttt ggcgaaaaaa agacccgcag agcgggcctt
     8941 gagataagca gaaaggaatg acttacagaa cgtcgatcgc gttcagttcc tggaatgctt
     9001 tttccagacg agcgatcatc gaagtctgac cggcacgcag ccatacgcgc ggatcgtagt
     9061 atttcttgtt cggctgatct tcgcctttcg ggttacccag ctgaccctgc agataagctt
     9121 cgttcgcttt gtagtagttc agaacgcctt cccaggttgc ccattgggta tcggtatcga
     9181 tgttcatttt cactacgccg tagcttacgg agtctttgat ttcctgagca gtagaaccgg
     9241 aaccaccgtg gaatacgaag ttcaggctgt tgtgcggcag gttgtgtttc ttagaaacat
     9301 attcctgaga atcacgcagg atggtcggag tcagaaccac gttacccggc ttgtaaacac
     9361 cgtgtacgtt accgaaggac gcagcgatgg tgaaacgcgg gctgattttg ctcagctcgg
     9421 tgtatgcgta atcaacgtct tccggctggg tgtacagtgc agaagcgtcc atgtggctgt
     9481 tgtccacgcc gtcttcttca ccaccggtgc aacccagttc gatttccaga gtcatgccga
     9541 ttttggacat acgctccagg tatttagagc agatttcgat gttctcttgc agagattctt
     9601 cagacaggtc gatcatgtga gaagagaaca gcggcttacc ggtagctgcg aagtgttttt
     9661 cacccgcgtc cagcagaccg tcgatccacg gcagcagttt cttcgcgcag tggtcagtgt
     9721 gcaggataac cggaacacca taatgttcag ccatctggtg aacgtgatgc gcaccagaga
     9781 tcgcgcccag gatagcagca ccctgcggaa cgtcagattt cacgccttta ccagcgataa
     9841 aggaagcacc accgttggag aactgaacga taaccggcgc tttaacttta gcagcagttt
     9901 ccagtacggc gttgatggag tcagtaccga cgcagtttac tgctggcagt gcgaagttgt
     9961 tttcttttgc tacctggaaa actttctgta cgtcatcacc agtgattacg ccaggtttta
    10021 cgaaatcaaa aatcttagac atgtctcttg tcctgtatcg tcgggcctta tactcataat
    10081 acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc
    10141 tcctggggat tcactctctt gtcgccttcc tgcaactcga attatttaga gtatgaaaaa
    10201 atgcgtgctc taaaagcgcg ctgaaacaag ggcaggtttc cctgccctgt gattttttac
    10261 ttcttagcgc gctcttcgag catcgctact gcaggcagta ctttaccttc cacgaattcg
    10321 aggaatgcgc cgccgccagt ggagatgtag gagattttgt cagcaatgcc gaacaggtcg
    10381 attgctgcca gagtgtcgcc gccgccagcg atggagaacg cttcgctgtc tgcgatagcg
    10441 ttagccacga tttcagtacc tttgcggaag ttcgggaatt cgaacacgcc aaccggaccg
    10501 ttccacagaa tggttttcgc attcttcagg atttcagcca gttcctgagc ggaagcatca
    10561 ccgatatcca ggatctgctc gtcagctttc acatcgttaa cagatttcag ggtagccggt
    10621 gcagtttcag agaactcggt tgctacgcga acatcagacg gaaccgggat gttgcaggtg
    10681 gtcagcagac gtttagcttc gtcaaccagg tcagcttcgt acagggattt acccacatcg
    10741 tggccttgtg ccgcgataaa ggtgttagcg ataccaccac caacaatcag ctggtcagcg
    10801 attttagaca gggagtccag aacggtcagt ttggtagata ctttagaacc accaacgata
    10861 gccaccatcg ggcgagcagg ttctttcagt gctttaccca gcgcgtccag ttcagctgcc
    10921 agcagcgggc ctgcgcacgc aacgtcagcg aatttaccga taccgtgagt agaagcctgc
    10981 gcgcggtgag cagtaccgaa tgcgtccatt acgaacacgt cacacagtgc agcgtatttt
    11041 ttggacaggg tttcgtcgtc tttcttctcg cctttgttga agcgaacgtt ttccagaaca
    11101 accagttcac cttcagcaac gtcaacgccg tcgaggtaat ctttaaccag acgaaccggg
    11161 ttagacagtt tgtctttcag gtagttaaca accggcagca gagagaattc ttcgttgtac
    11221 tcgccttcgg taggacgacc caggtgggaa gttaccatca cttttgcgcc ttgtttcagg
    11281 gccagttcga tggtcggcag agaagcacgg atacgcgcgt cgctggttac tttcccgtct
    11341 tttactggta cgttcagatc cgcacggata aatacacgtt tcccagcaag atccagatcg
    11401 gtcatcttaa ttacagacat ggtgaatcct ctcgttgatt ctaaaagttt tgcagacgct
    11461 gcttgcgtct tacctgaaag caacagtagc catagctaac gtcgtgtcga gcattcggtt
    11521 agcaaagccc cattcgttat cgcaccagac caacgttttg atcaggtgtg cgccactgac
    11581 ccgggtttgg gtgccatcga caatggcact gtgcggatcg tggttaaaat ctacagagac
    11641 caacggcaat tccgtatagt caactatacc atgaaatgca ccttgtgctg ctttttgcag
    11701 caacaggttg acttcattgg cttttacagg tttcttcacc gtcacgctta aatcgattgc
    11761 cgtcacattt atggttggca cacgtaccgc aatcgcttca aagcgatcgt taaattgcgg
    11821 aaaaaatcgt gtgataccag cggccagttt agtatcgacc ggaatgatcg actggctggc
    11881 tgcccgggtg cgacgcaggt caggatggta tgcatcaata acctgttgat cgtgcatggc
    11941 ggagtgaatt gtggtcacag tgccggactc aataccgtac gcatcatcta acaatttgat
    12001 gacgggaatt atgcaattcg tggtacagga agcgttagaa acgatgcggt gttccgcacg
    12061 aagttgatcc tgattgacgc cgtaaacaac ggtcgcgccg agatcgttac tgccaggatg
    12121 tgaaaagagc acttttttgg ccccggcggc aatatgcgct tcgctatgct cgcgggagcc
    12181 atatacgccg gtgcagtcga ggactacatc aacgccaagt tcacgccagg ggagcgattg
    12241 cagtgaacgt tcatgcaata cgcggatggc gtcatcacca acaaaaagtt gatcgcgttc
    12301 ctgtcgtact tcccatgcaa aacggccatg gctggtgtca tatttcaaca aatgcgccat
    12361 gcccgcagca tccgccagtt cgttgattgc caccacggta atttccgccc ggcgtccgga
    12421 ttcatacaaa gcacgaacca cattacgccc gatgcgaccg aagccattta tcgctacgcg
    12481 tacggtcata gatctcctgc aaggttttcc ctgagcaaat ttgccagaca gagtaatcta
    12541 gcaaatcgtc cggggaaacc ttacctgtcg caaactgcga ctgattggtt aattgtcgaa
    12601 catttaatcg actgaaacgc ttcagctagg ataagcgaaa cgtggaataa aaggaatgat
    12661 tgtccagtca aagaaagcaa ttatctgact tacgtcacgt tttcagcccc gcccctgtgg
    12721 aatttattcc actttgcaga atcacgaata agcgtagcaa aatcttttcg ttgtgccgtc
    12781 acaaaccctg acttatatca gaatatggct gatgcagcgt cgatattatc cgcaatacgt
    12841 ccgagtttcg gagtactcca atgatgacat tgccctaccc atattcttcc tccgcacgct
    12901 tttacgcact gcgcctcctc cccgggcagg aagttttttc ccagctacac gcttttgcac
    12961 agcaacatca acttcatgcc gcctggattg caggctgtac cggtagcctg actgacgtcg
    13021 ccttgcgcta tgccgggcaa gaaggcacca cgctcctgaa cggtacattc gaagtgatct
    13081 cactaaacgg cacgctggaa cagagtggtg agcatcttca tctctgcgtt tccgatccac
    13141 acggcacgat gcttggcggt catatgatgc ccggctgtac cgtgcggacc acgcttgaac
    13201 tggtgatcgg tagccttgag gaactggagt ttagccgcca accttgcgcg ctttctggtt
    13261 acgacgagtt gcatatttcc cccgttaaat aactactttc aaagaggttt tcttatggcg
    13321 cgtagccact tttccagtca ggcactggtg ttgatcgtta tatctatcgc catcaatatg
    13381 atcggcgggc aactggccag catggtcaaa ttacctatat ttctcgactc catcggcaca
    13441 cttattagcg cggtactgct tggtccggtg atcggaatgc tgaccggctt actgaccaat
    13501 ttactctggg gattgctgac cgacccgatc gccgccgcat ttgcacccgt cgcgatggtc
    13561 atcggcctgg tctccggttg gctggcgcga gcgggttggt ttcgcaccct gccaaaagtc
    13621 gttgtcagcg gcgtgattat tacgcttgcg gtaacggtcg tcgctgtacc gctgcgtact
    13681 gcgctgttcg gcggtgtcac cggtagcgga gccgatttgt tcgttgcctg gatgcactcg
    13741 atggggcaaa acctggtgga gtcggtggcg attacggtga taggcgcgaa cctggtcgat
    13801 aaaattctta cagcggtgat tgtctggctt ttactgcgcc agctgccgat tcgcaccacg
    13861 cgccattttc cggcaatggc tgccgtgcgc taaatgcatc catttacctc attaacactg
    13921 tgggcattag cggcctgcac cacgcttatc ctgcccgcgc agaccatttt gccgatctat
    13981 agcgcggcga cctttttctg ccttattgcg ctaaaggcca cgcggcggcg ggcaaaatat
    14041 gtcgtctggc tgatgttctc gctgggagcc gggctgtggc tggtacacgg tggctggcta
    14101 ccagaatggc taagcggcac gccacgctcg ccagaacggt ggtcgcatgc tattacgctc
    14161 tggttgctga ttctggcgat tgtttcaacc tcacagttgt gaatgcagta cgtccccgtg
    14221 caatgtttta ttcgcgctct gtttgcttct cgcctgccgc caggcgtcgc ttatctgttt
    14281 gccgggccgt tattggtcgt tgagcagtta aaacggcaat tagcgattat tcatgaagcg
    14341 cagcgtgccc gcggcgtgcc gctggatgag ggctggtatc agcgtttacg tgctatgccc
    14401 gcgttaatta ttccgctaac acacaatgcg cttaacgagc tggctgtccg tggtgcggcg
    14461 ctggatatgc gggcatttcg gatccacaac cgacgaacaa cgttgtgggc accagccgat
    14521 agcaccctac aacgcgtagc gcgttacacc atgatcttgc tgatgctgac agaattcgga
    14581 gcgtggatat ggttacgtta gaacagttcc gttattgccc gacacactca acgcatcctc
    14641 cgttctgtta tgactttcac tatgtcaaac cggggatggt cgccatcttt ggtgacaacg
    14701 gcagtggcaa aagtacgctg gcacagctga tggcgggctg gtatccggac tttctgccaa
    14761 gagagataac aggcacgggg acgctcctcg gtacgcccat tgggcatctc ccgcttaatg
    14821 aacaatccgc caccatccag ctcgtccagc aatcgcccta tttgcagctc tccggctgta
    14881 cctgtagcgt ggaagaagaa gttgcctttg ggccagaaaa tctgtgtctt gctgaagcag
    14941 agattatggc gcggattgat gccgccctga ctctgaccga gtgccagccg ttgcgccatc
    15001 gtcacccggc aacactctcc ggtggagaga cacagcgcgt ggtcatcgcc tgcgccatcg
    15061 cgatgcagcc aaagctctta attcttgatg aagcctttag ccgcctgacg ccgcaggcca
    15121 gcgaaatgct gttgcagcgc ttacaacact gggcgtttga acgtggttcg ctgattattc
    15181 tttttgaacg tcatcgcaca cctttcctga actactgcca gcaagcgtgg caattgcaaa
    15241 acggagcgct ccagccacta tgttaacgtt aaataagata tcttatcgct ggcctggcgc
    15301 ggcaacggat tgtctttgcg acatttctct gcaactcaaa caaggtgaat ggttggcgct
    15361 gaccggtgac aatggcgcag ggaaatcgac gctgttacga gtaatggcag gtcttcttac
    15421 gcccacagcc ggtacggtta tgctgcaaca acaggcgatg aaaaacctga aaaaccgcca
    15481 gcgcgcggca aaggttggcg tactgtttca ggaagcggaa aaccaacttt ttcacagcac
    15541 cgtcgctgac gagatcgcat ttggtttgaa actgcaaaaa tgcccggcgg atgaaatcac
    15601 tcaacgtacc catgctgccc tgcaatgttg tcagttagca gacactgcca actcgcaccc
    15661 gttggatctg catagtgcgc agcgacggat ggtcgccgtc gcctgcctgg aagcactctc
    15721 tccgccacta ttactgctgg acgagccgag tcgggatttt gacgaaaatt ggttgagcgt
    15781 gtttgaaagc tggctggaga agtgccgcca gcgaggcacg agcgtggtag caatcagtca
    15841 tgacgccgca tttacccggc gtcatttctc tcgtgtggtg cgactggaag atggtgtaat
    15901 caggaacgtt aatccaccag acgatattca cccgtagcgg tcatcattaa ggtcaaattt
    15961 gcaggcagac tctcttccag cacccggcga acattcggcc cgtcggtacg gtcataaaag
    16021 gcttctgcat ctgccagcga tacccctccc gccagcttgc gccccaccag acgctcccgc
    16081 aacgcagcag ctggcgcgtt gataaagatc gagaaatcac aaaattgcgc aagctggcac
    16141 cacttctcgt cgtccagcaa caaccagttt ccttcgacga tgacgagtgg tgcggtaacg
    16201 tgcaacgcat cttcaacagg atcatgcttt tgtcgatcgt actgcggcca cgtacaatcc
    16261 ccttccacca cctggcgcag attttccgcc agtttcgcaa cgtcaaatgt ctctggtgcg
    16321 cctttgaagg ggcgcaattg atgcgcgtcc agccagctat tgtaatggtg aaaaccatcc
    16381 atcgggagcg tctgaatagc gggcagctcc gggtcttgtt gcgcgagata ttcccagaag
    16441 gtggttaggg tagatttacc cgtccccggc ggagcgcaaa gaaaaaccac cgtacgccgc
    16501 tgcggattta ccgtctgtaa ggccgccagc atacgcagca acggtttatg cacgcgttct
    16561 atctcttcat catggtactg cgcttgcacg ttcagcccat tcaccgttaa ttcaattttc
    16621 acggcgttaa ttcctttaag gtggtcgtta ctgccagcgt cagcgccgtt ttgaccattc
    16681 cgctaaaacg cgcctctgac cagacggcga aatcggcgaa cttcatcgct tttaacgcat
    16741 cgtagagcgt cgcattgcga gttatactgt tgacattgct gataaaatcc caggttatat
    16801 catcggcaaa tccgggcgtt tcatcctgtt cgttcaggta atgccagtac tgggaaaagt
    16861 gcgtgatatc ggcatacagc catttatcca ttttccccag cgcataaatc aaacgcagcg
    16921 caacatcaat atcgtcaagc gggccgcttt gggcgagtaa cggcttcact gcatattcga
    16981 cgatctcttc atcgttatcg acaaacagcg acggcagaaa ctggcgtatc ccctggagca
    17041 aaatagaatg cgcagttttc ataaatgaaa ataaattgtc ctgtgcatcc agttgctcaa
    17101 gcacatcatc ttctgtcaga gtcgccattg gtcttccgat agatcaccat caatgcaggg
    17161 agtctattat attacacgtt tggtccgcgc cgccgatcag taatgtctgc gtccgcaccc
    17221 cattcgccgt ctgttggata ccgttcacca actcgccgcc cgtcacgccc gtagcgctaa
    17281 agaggatatc gttccccctc accagttcat cgagcgagta cacacggtta acgtcaacgc
    17341 ccattgcctt acaacgcttg cgctcctgct ctgcaatctg ccgattttcc gtgtaatccc
    17401 ctttagcctg acaaaagtca atcagctcgg cctgcatatc cccgcctaac gctttaacgg
    17461 cacaggcaga aatcacgcct tctggcgcac cgccgatggt gtacatcaca tcgtaaggat
    17521 tatcctgcca gcaggtcagc acgctggcgg cgacatcgcc atccggcagg gcaaaaacct
    17581 tcacgcccag ctgtgttgct tcttcaatcg cggcactcag acgcggttta tccagcgtaa
    17641 ccatgcgcag cttgtccagc ggtttaccta acgcccgcgc cacgttacgc aagttgtccg
    17701 ccagaggcaa agacagatca atcgccccgg cagccagtcg attaacaacc agctttttca
    17761 tatacatatc gggcgcatgt aacaggctat ctcgaggagc gaaagccatg accgccagcg
    17821 cattgctctg ccccatcgcc accatccgcg tgccttcaat gggatcaacc gcgatatcga
    17881 cttctggccc atcgcctttg cccacctctt cgccaatcca cagcatcggc gcatggtcaa
    17941 tttctccttc gcagataacc actcgtccac gaaaagcgac gtcgtttaat gcctggcgca
    18001 tcgcggtgac cgccaggcca tcaattttat ttttgtcgcc acatccggtt tgcggccagg
    18061 cagccaacgc ggcctgttcc gtaacgcgaa ataatggcca cgccagggac atcattgcgc
    18121 ggcctccccg atatcaacgc caaattgtgc cagcagatat ttctcggcct gttcattcca
    18181 gatgccgtgc gtttcctcaa ccagccgcgc caactcttta aacagcggat cggttttgcc
    18241 tttttcggca aaatcggcaa tggcagtgag cggcatggtc acgccgttat aaatgagttt
    18301 tttaccgcca gggatatccg gcagattgag cacggtttct ggcaccgcat ccaggccacc
    18361 aatatgggtc accataaacg acggctgtaa ctgtcctgtg gcgctaaggg caatcgcctc
    18421 tttcatgtcg tccgttgaac cgccggatgt accgactacg tgcgtgctgt tgtaatggac
    18481 gttgtagaaa ttaaacggca ctttgaagtt tttatccgtc agcccggcaa agaagttcag
    18541 acagccatct tccgccagta attcgtcagc catctcaacg acagcaggca ccgccgcata
    18601 aacaaaaatg tcatcgaacc cgacatctcc tgtcagcgcc cgcagcatct ggataggatc
    18661 gctcatccct ttggtattca catacaccag ctcaatgcct ttactggccg ccagatccac
    18721 cggcagcagc ttctgcacct gcgccaggcg tttgtcgtcg atatcgacca ccaccacccg
    18781 tgacggttgt atgccaccgt taatggcgta atcgatagcg ccaatg
//
LOCUS       DAJTYI010000075        18241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYI010000075 DAJTYI010000000
VERSION     DAJTYI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..129)
                     /gene="flgE"
                     /locus_tag="OO564_002476"
     CDS             complement(<1..129)
                     /gene="flgE"
                     /locus_tag="OO564_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="the hook connects flagellar basal body to the
                     flagellar filament; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6653566.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMF"
     gene            complement(154..849)
                     /gene="flgD"
                     /locus_tag="OO564_002477"
                     /pseudo
     CDS             complement(154..849)
                     /gene="flgD"
                     /locus_tag="OO564_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(861..1264)
                     /gene="flgC"
                     /locus_tag="OO564_002478"
                     /pseudo
     CDS             complement(861..1264)
                     /gene="flgC"
                     /locus_tag="OO564_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(1268..1441)
                     /locus_tag="OO564_002479"
     CDS             complement(1268..1441)
                     /locus_tag="OO564_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR6653567.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            1596..2255
                     /gene="flgA"
                     /locus_tag="OO564_002480"
     CDS             1596..2255
                     /gene="flgA"
                     /locus_tag="OO564_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6653568.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            2331..2607
                     /gene="flgM"
                     /locus_tag="OO564_002481"
                     /pseudo
     CDS             2331..2607
                     /gene="flgM"
                     /locus_tag="OO564_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            2612..3028
                     /gene="flgN"
                     /locus_tag="OO564_002482"
     CDS             2612..3028
                     /gene="flgN"
                     /locus_tag="OO564_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6653569.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(3068..4603)
                     /gene="murJ"
                     /locus_tag="OO564_002483"
     CDS             complement(3068..4603)
                     /gene="murJ"
                     /locus_tag="OO564_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6653570.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(4770..5636)
                     /locus_tag="OO564_002484"
     CDS             complement(4770..5636)
                     /locus_tag="OO564_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6653571.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(5638..6285)
                     /locus_tag="OO564_002485"
     CDS             complement(5638..6285)
                     /locus_tag="OO564_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6653572.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(6296..6880)
                     /gene="rimJ"
                     /locus_tag="OO564_002486"
     CDS             complement(6296..6880)
                     /gene="rimJ"
                     /locus_tag="OO564_002486"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6653573.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            7116..8324
                     /gene="mdtH"
                     /locus_tag="OO564_002487"
     CDS             7116..8324
                     /gene="mdtH"
                     /locus_tag="OO564_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6653574.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            8388..9035
                     /gene="grxB"
                     /locus_tag="OO564_002488"
     CDS             8388..9035
                     /gene="grxB"
                     /locus_tag="OO564_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6653575.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            9169..9729
                     /locus_tag="OO564_002489"
     CDS             9169..9729
                     /locus_tag="OO564_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6653576.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            9835..10881
                     /gene="pyrC"
                     /locus_tag="OO564_002490"
     CDS             9835..10881
                     /gene="pyrC"
                     /locus_tag="OO564_002490"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6653577.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            10955..11200
                     /gene="dinI"
                     /locus_tag="OO564_002491"
     CDS             10955..11200
                     /gene="dinI"
                     /locus_tag="OO564_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6653578.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            11490..11744
                     /gene="bssS"
                     /locus_tag="OO564_002492"
     CDS             11490..11744
                     /gene="bssS"
                     /locus_tag="OO564_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6653579.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            11859..12977
                     /gene="solA"
                     /locus_tag="OO564_002493"
     CDS             11859..12977
                     /gene="solA"
                     /locus_tag="OO564_002493"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6653580.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            13025..13138
                     /locus_tag="OO564_002494"
     CDS             13025..13138
                     /locus_tag="OO564_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6653581.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            13399..13965
                     /locus_tag="OO564_002495"
     CDS             13399..13965
                     /locus_tag="OO564_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6653582.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            13969..14544
                     /locus_tag="OO564_002496"
     CDS             13969..14544
                     /locus_tag="OO564_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6653583.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(14586..15638)
                     /locus_tag="OO564_002497"
     CDS             complement(14586..15638)
                     /locus_tag="OO564_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6653584.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            15863..16783
                     /gene="lpxL"
                     /locus_tag="OO564_002498"
     CDS             15863..16783
                     /gene="lpxL"
                     /locus_tag="OO564_002498"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6653585.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            16955..17152
                     /locus_tag="OO564_002499"
     CDS             16955..17152
                     /locus_tag="OO564_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6653586.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            17176..>18241
                     /locus_tag="OO564_002500"
     CDS             17176..>18241
                     /locus_tag="OO564_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6653587.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLAL"
BASE COUNT         4603 a         4626 c         4495 g         4517 t
ORIGIN      
        1 aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg agttggcgat
       61 attgttgcca ataacatcga ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga
      121 aaaggccatg actgactcct gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag
      181 ggtggtggtg ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc ggatcacacc
      241 ctgcaccaga gcaaactgca gcggctgggc aaccagttgt gtaccaccgt tactggcgct
      301 aatcgctaca ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg taccgtccca
      361 ggtgaaactg tgaactccgg cggtcagttc accaatatca atggtgcgca caaccgcgcc
      421 atttttatcg gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa
      481 cggcgtggtc gtggtcacag ccccttcttc actgccggtt cccgcaagaa cagttgtgcc
      541 ggggatcatc acgccgtgac cgatcaggtt actggcctgt aacgactggc tgttatcaat
      601 ctgtccggaa atagatccga gcgtggtatt gagtttttca atcccgctga ccgtgctgat
      661 ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt cctagttttt
      721 cagctgcgcc accagcaaag tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt
      781 gagcgaacta ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc
      841 aatggacata gctttctcct ttattgaccg agcgtaaggg ttttcagcat catgcttttc
      901 accgtgttga gcacttcaac attggcctga tagctgcgtg acgccgacat ggtgttaacc
      961 atctccccga cgacatcaac gttcggcatt tttacgtagc cctttgcatc tgccagcgga
     1021 ttacctggtt cataaaccag tttgtccggg gcctgacttt cttaacatcg gcaaccttta
     1081 cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt tttgcccgat
     1141 atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg gccgccacgt
     1201 tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata ttcagcagtg
     1261 ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc ccgctcaacg
     1321 cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgact tcctgacgct
     1381 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
     1441 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
     1501 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
     1561 tacaactcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg
     1621 cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca tcgcaattgc
     1681 acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
     1741 cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc aataattccc
     1801 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
     1861 ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcctattgtg cggggaggaa
     1921 agctggaagc tggcaatgtc aaactgaaac gtggacgcct ggataccctg ccaccgcgta
     1981 cggtgctgga tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc
     2041 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
     2101 atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
     2161 cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg
     2221 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
     2281 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg
     2341 atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc actgacgcgc
     2401 cggtaacgaa cagccgcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa
     2461 ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg
     2521 attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa
     2581 gcgcagcaag acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca
     2641 gatgtccgct gtgcttaacg atctcaaaac ggtaatggat catgagcagc aacatctctc
     2701 tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct
     2761 ggcgacgctg gattacctcg aacagttacg caagaaagaa cccaatacag caaatagcgt
     2821 tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa
     2881 tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat
     2941 gttgaaaccg catcaggaac cgacgctata tggggcgaac ggtcagacct caacacccca
     3001 tcgcggcagt aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa
     3061 tgcattgtta caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg
     3121 cagcgaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg
     3181 gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca
     3241 tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa
     3301 agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac
     3361 caatagaaag tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt
     3421 gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtct tggcgggaat
     3481 aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa
     3541 ttaacgcccg ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg
     3601 aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagt gccaacagga
     3661 aacaaagacg caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa
     3721 aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg
     3781 gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg
     3841 aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg
     3901 gtcccatctg tttcaccacg cgcattgctc cagcatcgtg gaagttaatg cgcggcagga
     3961 ccagcatgcc gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga
     4021 ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg
     4081 cgaaaccaat catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt
     4141 tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa
     4201 aggtaatctt tagtaggtgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg
     4261 gcgcggtcac catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca
     4321 gcgcaagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct
     4381 gcttactttt atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa
     4441 agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc
     4501 ctgcgccaaa gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca
     4561 tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccgga
     4621 cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctagatta
     4681 tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc
     4741 ttgtcaacga tacgttgcgc cagcactgcc tattcgccgg cggtttgcgg aactgtctga
     4801 ttttgcacgc attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg
     4861 ctctgccaac caggaatcgg tttattcact acaccctgcc cgcgctcctc acgccattcg
     4921 cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga
     4981 cttccggcac ggcgatgcat acaagtggtg atctgcaaag gaccggccga aaaatggtgc
     5041 tcggcaaaca gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct
     5101 ttgccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg
     5161 aaataaagat cgtgtggccc gacgctattg ctacgatgtt tatccattct tagcgaggct
     5221 gcggtggcga gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg
     5281 accatcaacg tcagtttttt ccgcgccgcc agttccacca gcctttcagc atcgcgcaga
     5341 ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa cgtactgacc
     5401 acgtcaaagt ggctggcggt gctggagtgc acaaaaaccg catcacaact ggcggcaagg
     5461 ctggataacg aatcggcata aggaatgcgc cagctgtcac aaattggcag ggctttggcg
     5521 cgcgtaggcg accaggctcc ttgtaacgtc cagtcagacg ctgccgccag caccggtaac
     5581 cacgcttttt gcgcaatgcc gcctaatccc actacgccga tacgtaattt tttcacttta
     5641 atctcccaga tgggccagca acgaatcaag acgttgtttc agttctgcca cttcgatttc
     5701 cagggcttcg acgcgggtct gtaaatcacc gtcaaccatg ttcgacatag ccgttaccgc
     5761 cggctgatct tcaacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg
     5821 ttcgcgggcc agacgcacca caaaaggacc atcttcgcga ttcgccagtt gttccagcgt
     5881 cgactccact tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc
     5941 acccagcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt ctgcggcgct
     6001 cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg tgacccgatt
     6061 accaaaaccg ctcactgtgc gtagataatg acgtttgacc agattatcca gctgttcctg
     6121 cacttcggat tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt
     6181 gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa
     6241 gcaaccaatt acgcgagctt ccagggcagt aagttgatat ttcatgagat tctccttagc
     6301 ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat
     6361 caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat
     6421 caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc
     6481 gctgcatata acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt
     6541 tttgcccaat cgaataaccg agatagcagg catgaaaaga gccgcgaaca acattggaaa
     6601 aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg
     6661 aaccttgttt atgaaattcg ttaatcatcc ctagcctggc ctgccagcct gatggataac
     6721 agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat
     6781 aatccgccag acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg
     6841 ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg
     6901 taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt
     6961 ccaaaatgcc attttctgca ttattcacat atttgataaa aactctctat caaagcgacg
     7021 ttttgataaa aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat
     7081 agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga
     7141 acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg
     7201 tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg
     7261 gtattgctct cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa
     7321 ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg
     7381 gattcgccac aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct
     7441 cgggactcgg cggcacgttg tttgatccgc cgcgttcggc gttggtggtg aaactaatcc
     7501 gtccacaaca acgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccagtgcgg
     7561 tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca
     7621 caggggccgt tttgtttgtg ctatgtgcgg cgttcaatgc gtggttgtta ccggcatgga
     7681 aactctccac cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc
     7741 gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc
     7801 tgatgctgcc aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt
     7861 atgccattga agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa
     7921 agcattttcg tctggaatac cggttgatgg ctgggctgtt gataatgtca ttaagcatga
     7981 tgccggtggg catggtcagc ggcctacaac aacttttcac cctgatttgt ctgttttata
     8041 tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa
     8101 gagctcgcgg cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg
     8161 gttatatcgg tggtggctgg ctgtttgacc tgggcaaatc gacgcaccag ccagagcttc
     8221 cgtggatgat gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta
     8281 gtcagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat acacccccct
     8341 gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca
     8401 tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc
     8461 ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc
     8521 aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata
     8581 tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg
     8641 ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt
     8701 ttgccaaatc ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca
     8761 agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta
     8821 agaatatcag cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga
     8881 atggcgaact ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg
     8941 tagccggaat caactggcca agccgcgttg ctgattacca cgataatatg gcgaaacaga
     9001 cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta
     9061 ttcgcgggtt gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat
     9121 gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt
     9181 ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa
     9241 tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa
     9301 gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaactt gaccagccaa
     9361 attggtcgcg aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac
     9421 tccctgttcg gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt
     9481 gataaagaaa aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa
     9541 ccggaaaaaa tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc
     9601 aattacttta accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca
     9661 atggcgaaaa aactggcgaa aggcattgaa gtgaagccag gtgaaattgt cattccgttt
     9721 acggattaat gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacatttc
     9781 cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact
     9841 gcaccatccc aggtattaaa gatccgccgt ccagacgact ggcaccttca cctccgcgat
     9901 ggcgacatgt tacaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta
     9961 atgcccaatc tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt
    10021 attcttgacg ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca
    10081 gattcgctgg atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca
    10141 aaactttacc cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca
    10201 atcatgccgg tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa
    10261 gtgacacatg cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg
    10321 gaacctctgc gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa
    10381 gatgctgccg actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag
    10441 catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt
    10501 ttacccatcc tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt
    10561 tttaatcgag tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc
    10621 agttgcggct gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc
    10681 tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag
    10741 ttctatggct tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt
    10801 gctgaaagca tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta
    10861 cgctggtccg ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg
    10921 tataaataac cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa
    10981 aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat
    11041 tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa
    11101 tttatcggtt attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga
    11161 aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt
    11221 tgccgggtcg ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac
    11281 ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt
    11341 catataaatc ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc
    11401 cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt
    11461 tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc
    11521 atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt
    11581 tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct
    11641 ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa
    11701 ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc
    11761 acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt
    11821 aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta
    11881 ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg
    11941 tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc
    12001 gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgttagtc ctccgcgcgc
    12061 aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg
    12121 gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg
    12181 aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag
    12241 aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca
    12301 gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt
    12361 tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg
    12421 acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc
    12481 tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc
    12541 gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat
    12601 attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg
    12661 ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggccagtgat gggtcagaag
    12721 ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct
    12781 gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata
    12841 atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg
    12901 aaatagctgc cgattttgcg caagaccaaa aaagcgattt tgatttgacg ccattcaggc
    12961 tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta
    13021 tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta
    13081 tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt atttttagtt
    13141 ttccgcactg acataaactg acaaatattt aatctccctt tcatttagcg tgaaaaattg
    13201 aatgagatta ttcctgcttc tacagcactc accagacatc tctgatgctg aacccactaa
    13261 acttatgaag attctttcat tattaattca aaattattga aataaaaata aatatcattc
    13321 aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa
    13381 ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct
    13441 gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt aggtctgtgg
    13501 atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc
    13561 atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca
    13621 ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa agctggacat
    13681 ctcgcattgt acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc
    13741 gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct
    13801 ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta
    13861 ctgtctgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact
    13921 ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc
    13981 ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat
    14041 tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcataca gcatcttggc
    14101 tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt tgatgaaaaa
    14161 aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaaccac
    14221 gccgaacgcg ataaacatct tcgcagtgcg gattttctca ataccacaaa atatccacag
    14281 gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat
    14341 ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc
    14401 gacgacccat ggggcggtaa acgtgcaggc tttgaggccg aaggcaaaat taagctcaaa
    14461 gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca
    14521 gtggaaggcg tacagcagaa gtaagagtga aatgttgccg gatggcacgc atccggcaat
    14581 gatatttatt ctgttggatc aggaataccc agtgttgtat tcagacgtcc acgagactta
    14641 ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt
    14701 aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc cgcgcatact
    14761 ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac
    14821 ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc
    14881 tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta
    14941 cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc gttatgtttc
    15001 atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac gattttttta
    15061 tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc
    15121 ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt acgcatgtcg
    15181 ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg cagatactca
    15241 ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt
    15301 accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt
    15361 aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc aacattgctc
    15421 gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg cccaaaaaca
    15481 ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg
    15541 aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat tttggctttt
    15601 aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc ctgcgagatg
    15661 ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc cacacatttt
    15721 acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat ttcgcccagt
    15781 cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca attgcccgta
    15841 taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg cactgcttca
    15901 tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttggtcg tgcaattgcc
    15961 ctacccgatt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac gttttatgaa
    16021 acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca
    16081 agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc tgatggaaac
    16141 cggtatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag tgatcggcat
    16201 ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg gcatccattt
    16261 tctgacactg gagctgggcg cgcggcagtt tggtatgcag gaaccgggga ttggcgttta
    16321 ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt tgcgctcaaa
    16381 taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga aaaaaggcga
    16441 agtggtctgg tacgcaccag atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt
    16501 atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac ggatgtccgg
    16561 cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt atcaattgat
    16621 tatgctgccg ccagagtgtt ctccgccact ggatgatgcc gaaactaccg ccgcgtggat
    16681 gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt ggttacaccg
    16741 tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc atgccggatg
    16801 cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga taaaagctct
    16861 ctggattgcg ccccctggaa gtcgggcgca taattagtgt gtttatcttt tcttcttatg
    16921 ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa atgacacccc
    16981 tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta ccggggccgc
    17041 cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggtg ttaccggcca
    17101 ctccgccctg aatatgtggt ccggtattgt cttcagtata atgggttcct aaacacatca
    17161 ttttagatat tgaatatgag acgggcaatc agctacatca gattttcaag tgaacgacaa
    17221 ctgaaagggg actctgtacg ccgacaatct aagctggtta ctgactggct tgataagaac
    17281 cctgagtttt acttggattc atccttgagc tttaaagatt tggggaaaag tgcttttagt
    17341 ggtaagcacc tgaaaggtgg tctaggtgac tttttaaccg ctatcgagaa agggcttgtt
    17401 aaagctggtg atacgctact aatcgagagt ttagaccgtc tgtcacgtca ggatattgat
    17461 attgcttctg agctattacg ccgtatctta agggctggtg tagatgtagt cacactgagc
    17521 gatggagaac attacacacg agaaagttta aaagacccgt tagccctgat taaaagcatt
    17581 ctgataatgc agcgggcgca tgaggaaagt ttgaggaagt ctgagcgtgt ccaagctgca
    17641 tggaaccgta aaaaagaact catctccgaa ggtatcaagg tatctaggcg ttgtcctgca
    17701 tggttaaggc tgaatgatga ccgtcgaaca ttcacaatca ttcctgataa agtggaagtt
    17761 gttaaacgag catttgacct gagattacag ggtttaagct tttgggctat caccagaact
    17821 ttaaacgatg aaggtcactt gtcattgaat caatacacac ccaaacaaaa ggggtggtct
    17881 gatactgctg taaagaaact actcagaaat cgtgctgtga ttggatgttt cacacctgct
    17941 ggacgtgaag aagttcaggg ttactacccc gcaataatca gtgagtcact attctatcga
    18001 gtacagcaac tgaacacagg gcaatatggt agggcttctg tatcaagtaa tccgctatca
    18061 gtcaacttgt tcaggggtat catcaaatgt tctgagtgtg gggctactat tgttcttggt
    18121 ggatatgcct taaaacgttt tggaatgtac agatgcccta atcgttcagc taacaggtgt
    18181 agtgcaaagg ctatcagtcg taagcaaacg gatgcaacta tcctttacat gctcgcatta
    18241 t
//
LOCUS       DAJTYI010000076        17612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYI010000076 DAJTYI010000000
VERSION     DAJTYI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..754)
                     /gene="aroD"
                     /locus_tag="OO564_002501"
     CDS             complement(<1..754)
                     /gene="aroD"
                     /locus_tag="OO564_002501"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6653588.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQ"
     gene            complement(785..1651)
                     /gene="ydiB"
                     /locus_tag="OO564_002502"
     CDS             complement(785..1651)
                     /gene="ydiB"
                     /locus_tag="OO564_002502"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6653589.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1663..2934)
                     /locus_tag="OO564_002503"
     CDS             complement(1663..2934)
                     /locus_tag="OO564_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653590.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3117..4321)
                     /locus_tag="OO564_002504"
                     /pseudo
     CDS             complement(3117..4321)
                     /locus_tag="OO564_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4421..4777)
                     /locus_tag="OO564_002505"
                     /pseudo
     CDS             complement(4421..4777)
                     /locus_tag="OO564_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4913..5020)
                     /gene="rprA"
                     /locus_tag="OO564_002506"
     ncRNA           complement(4913..5020)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO564_002506"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5206..6318)
                     /gene="ydiK"
                     /locus_tag="OO564_002507"
     CDS             complement(5206..6318)
                     /gene="ydiK"
                     /locus_tag="OO564_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6653591.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6707..9763
                     /locus_tag="OO564_002508"
     CDS             6707..9763
                     /locus_tag="OO564_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6653592.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9760..10170
                     /gene="menI"
                     /locus_tag="OO564_002509"
     CDS             9760..10170
                     /gene="menI"
                     /locus_tag="OO564_002509"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6653593.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10270..10458
                     /locus_tag="OO564_002510"
     CDS             10270..10458
                     /locus_tag="OO564_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6653594.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11006..11374
                     /gene="sufA"
                     /locus_tag="OO564_002511"
     CDS             11006..11374
                     /gene="sufA"
                     /locus_tag="OO564_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6653595.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11383..12870
                     /gene="sufB"
                     /locus_tag="OO564_002512"
     CDS             11383..12870
                     /gene="sufB"
                     /locus_tag="OO564_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6653596.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12880..13626
                     /gene="sufC"
                     /locus_tag="OO564_002513"
     CDS             12880..13626
                     /gene="sufC"
                     /locus_tag="OO564_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6653597.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13601..14872
                     /gene="sufD"
                     /locus_tag="OO564_002514"
     CDS             13601..14872
                     /gene="sufD"
                     /locus_tag="OO564_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6653598.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14869..16089
                     /gene="sufS"
                     /locus_tag="OO564_002515"
     CDS             14869..16089
                     /gene="sufS"
                     /locus_tag="OO564_002515"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6653599.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16102..16518
                     /gene="sufE"
                     /locus_tag="OO564_002516"
     CDS             16102..16518
                     /gene="sufE"
                     /locus_tag="OO564_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6653600.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16667..>17612
                     /gene="ldtE"
                     /locus_tag="OO564_002517"
     CDS             16667..>17612
                     /gene="ldtE"
                     /locus_tag="OO564_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6653601.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPS"
BASE COUNT         4623 a         4281 c         4537 g         4171 t
ORIGIN      
        1 cctgatgtaa aatagttaat accgtgcgca aatcatttac cgagatttgc cctggcgcag
       61 aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca gccagacgag
      121 aaattacgcc agtttttgcc atcgacatcg tgatgattgg acgatcggca tactgctctt
      181 gcatctccag ggtcgcggca agcaacgtca gcacatcgct ggtactttgc ggcatcagcg
      241 caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca atgatttctt
      301 cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca tcatgcgcgt
      361 gggcgtaggc gacggtttct ttgacctgat catcgccggt aaataactcc agatcgatca
      421 tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc tcggtggaaa
      481 tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt ttttctggca
      541 tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag aggtcggcaa
      601 agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg agagcttcgg
      661 atttcacgcg ggcgatatct ttcgccatca gcgagacgat gattttaggt gcgcccgcac
      721 caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc acgcggtcag
      781 cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa tctttgccag
      841 tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca tcaatcgttt
      901 tgcaaccagc ttgttgtgcc tgctgcaata acttcgtcat atgcgggtta tacacgcatt
      961 cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat tcattctcaa
     1021 ggggtttcat acctactttt gtgccattgg ttaaaatgtc ggcggaagcc agggcttcag
     1081 caaaggcttg ctgatcggcg agatcggtaa ccgtgacgac acaatcggtg ttttcgttaa
     1141 cccgctgcgc gaaggcgagg gctttatcga agaactcatc ccgacggtta aagagtttaa
     1201 tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca cctccggccc
     1261 ctaacagcac catcgttttg cctttgatat caaaaccgct ctctttaatg gcgcgaatat
     1321 ggcccgtgcc gtcggtgtta tagccacgca gatagccatc atcattaacg atggtgttga
     1381 tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac gccagttgtt
     1441 tgttcggcat cgacacacca gttccgcgca ttttgagggc ttttaatcct tcaattgctc
     1501 cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat cccgcttttt
     1561 ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa gccatcaatc
     1621 caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc tatgcttaat
     1681 tgtgtttaac cgggtgctaa atttgcgttc gccaaaccgc acatcattct caggaataat
     1741 aaaaacgcgg taatagcggc taaaaacaat aattgcggta atcagggcca gcagcgcgaa
     1801 agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac cggtaatcag
     1861 cggaataaca aagttagcca gtccacccat catcatataa atactggtga ctttggcttt
     1921 gcttttgggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc cgccagctgc
     1981 tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt acaggtagat
     2041 aatggcagct gttattgttg ccagcgcaga gttaaataca ttagcccaga tgggccggac
     2101 catttttttc agtagtgcgg caaaaataaa gacacagacc aacgagccca tactgtaata
     2161 agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg catatttggg
     2221 catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca gtaccgatga
     2281 aacaccttcc agccagacta acggtttgct gttcatttgc ggtaattcat tagctacgct
     2341 ggcgtccacc aactggctgg ggaatttgct tttcaacagc atcagcgtga tcagtacaaa
     2401 cagaataccc ggaataatca gcccatagcc gtaccagata ttattgagca acatatagct
     2461 caccagcatt gggtagaaca tttgcccaaa tgacaccatc gctttaacca gtatgaccgc
     2521 cgaaccagag gctttcggaa agcattccat gagcgcgggg tagccacccg tatccagcgc
     2581 tgagttagcg atacctacgc acactgccag accgtaggcg agagttaaat tcgggcaagc
     2641 gggaatacca aagaagaata gcagatacat tattactgcc attaatatca ccgcccgacg
     2701 accaaactta tcggagatca caccgaagaa taaaatactg atcaatcgcc ccaaaccgat
     2761 accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa aacttttctg ccagagatgt
     2821 catattttgg gcaagcgtaa taacactaat gccgtgcagg aaatagctga agtaaataca
     2881 aagaacagcc aggataaatg gcgtgctgaa agataatttc gttaccgtac gtattgttct
     2941 ccgacggttt acataattac tatactgaag atgattttcc atgggtacat ggcataaagt
     3001 ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt tccttagtta
     3061 gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat attgcattac
     3121 ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg gctgcgtaag
     3181 ccgataaaca gtgccagtaa aaaaccgatg gcagtatcga accacataat atcggcaata
     3241 cttctttggg acagatgagc cgtaatcaac ggaatagtaa aggtcgcaat actgcccgca
     3301 ctgtaataga tccctgtagc tttaccttta gcgtaaggga aacgttcagc tattaacgtc
     3361 aggccaattt gcacaacacc tccagcagag gtaaaaccaa tgacaaaagc aaatattatc
     3421 accacataaa atgtgggatg caggcagacg gtaaacagag caataaatga gataaaggtg
     3481 tacagcatca gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc ggtaataaat
     3541 acacaaagca gcgaacccac ggtgtagata ctgagtagtt tgatcgacat agtgtatgac
     3601 atgcctgcaa caaattgtcc gtactgtgcc agccactggc taaccagata aaacgttgcc
     3661 attgagatat agccatataa ggtataactg gctaaatcga taattgaaca gcgatgttcc
     3721 gtagagctgg tggttttctt tatgacaggt aagcgacgac ccggatgggg tgggaacgta
     3781 caacggtata aaaacagagc gttaataaac ataatgcctg cagcaatcat aaaggaccaa
     3841 ccgaaccaca gttcagccca cactaacagg ctaatgatta gcggtaataa aaattgtccg
     3901 ctggaaacaa atgctttaat taaaatattg gctgtcccag gtgagcgtgg aaaagcttcc
     3961 atcaaactgg gataagtgcc tgcatcgaga aaactgtttg ccattcccgc cagaaagcca
     4021 aaaacataag cgataatgat gttattggtc tgcaggatgc caaaaaagaa ggccatatag
     4081 cagcacattc cgagcatgat aaaagggcgg cgaccaaagc gatcggataa taatcctgca
     4141 aaaagcaaga cacttaatcg accaatgccc agcgatgaga taactatcga gacacccgcg
     4201 gcattagtct gacaaagtgt ctccagcgag gccatattca ggctcatcaa aatgacgccc
     4261 ataccatgca ccaggtaata aaatacaacc caagtgcggt agggtaatag ggatttttca
     4321 taggttctac ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca gtatccattc
     4381 attattgttg taacaacgac aattcctttc tgtggaaaaa ttaacaaagc cgctccagat
     4441 cgtgtgcata taattcagcg gctaccgatt gatagatctt ccagtcaata aaattgccat
     4501 cagggtagac ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg ttgttgccga
     4561 tgcggttatt tattttctct atgatggcat gaagatgttt ttttcgttac tgacgcatag
     4621 ccaacagttg ctcgactaca caatcaggaa tggcgcattt gccattttcc cactgacgcc
     4681 agctttcact gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa
     4741 aaatatggcg gagtgcctgg agttcataag cgttcatcgt gtaatggctg catgaaataa
     4801 acaataaaat ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct
     4861 tgtgcaacat tattggaaag agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa
     4921 aagcccatcg tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg
     4981 atttccatgc ttataaatca atatgttgat ttataaccgt gagctaatag taggcatacg
     5041 gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca
     5101 ggtactacca gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa
     5161 ttagcggtga ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcggttt
     5221 ctcgatttcg ccaagttctt cgagaatttc ttccggttgg tccgtcggcg gcgggacttc
     5281 ttccacccac gcggcaaaca gacgccagga aacggctaac agaaccggac caataaacag
     5341 accgatcatc ccgaaagcaa tcaaaccacc aataacgcca gagaggatca ggatcagcgg
     5401 taaatcggca cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac
     5461 accgctccac actaacaata ccgttcccca ggtggtatcg ccggtccagt agagccagat
     5521 aatcgccgga atcagtaccg gcaacgggcc aagctggaca aggcaggaga ggatcattaa
     5581 caccgttagc agcgttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac
     5641 tgcctgtact aacgccgtca ccaccacacc cagcgccacc gcgaggatag cctgtgccgc
     5701 cagcagcacg gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc
     5761 ttgtgccacc tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca
     5821 atgcaccata aagcgcccga tatgcgccgc ctgcccaacg aaccaagtgg tggtggtgcc
     5881 aatataaggg cggactttcg ccatgatcgc cgtacccccc atatccagca agttgtgcca
     5941 gcctgcatac agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa
     6001 cgtcatgtcg ccgctggaaa tggctttaat tagcgggccg ctgccgtcga cgatactgtt
     6061 aaccagcaaa gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac
     6121 ggcgagagag cggcggccaa acatgatctt ttgcaaacgt aacaataccg gccaggtggc
     6181 gataaccacc gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca
     6241 tgccacaatc atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg
     6301 ctgacgaaca tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga
     6361 cgtggacacg cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggcc
     6421 gattctgtcc ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac aactcaggca
     6481 acacgcaaac gatttactcg tcgtatttca agttgcatgt gctgcggcta cgttcgttca
     6541 ccccagtcac ttactattgt aagctcctgg agttcactcg cttgccgcct tcctgcaact
     6601 cgaaatccat agagtaaata aacatcacct ttcagtgcgc tattgcagcc gcctgaaaga
     6661 atagtgtatc gacctacatc acagaccgca ggaaagggtc aatataatga ttccacagat
     6721 ttcccaggca cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc tggagcaaca
     6781 aggttttacc ggcgatgcgg cgacaagtta tgccgatcgt ctgacaatgt cgaccgacaa
     6841 cagtatttac caacttctcc ccgatgcggt ggtatttccg cgttcaaccg cagatgtggt
     6901 gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta ccccccgcgg
     6961 cggcggcacc ggcactaacg gtcaggcgct caaccagggg attattgttg atatgtcccg
     7021 ccatatgaac cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg
     7081 ggtgataaaa gaccaactca atcagtacct gaaaccgttc ggctactttt ttgcgccgga
     7141 actttcgacc agcaaccggg caacgctcgg tgggatgatc aataccgatg catccggtca
     7201 gggatcgctg gtctatggca aaacgtcaga tcacgtactt ggcgtacgcg cggtgttgtt
     7261 ggggggcgat attctcgata cgcaaccttt acccgtcgag ctggcggaaa cgctgggtaa
     7321 atccaatacc acaatcgggc gaatttataa cacggtttat caacgttgcc gtcagcaacg
     7381 ccagttaatc atcgacaact tccccaaact taaccgcttt cttaccggtt acgatctgcg
     7441 tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg cgcattctga cgggttcaga
     7501 agggacgctg gcctttatta ccgaagcgcg gctggatatt acgcgcttgc ctaaagtgcg
     7561 ccgtctggtg aatgtcaaat atgactcttt tgactctgcg ctgcgtaacg cgccgtttat
     7621 ggttgaggcg cgggcgcttt cggtagagac ggtggactca aaagtgctga atctggcgcg
     7681 ggaagatatt gtctggcatt ccgtcagcga gttgattacc gatgtgcctg acaaagagat
     7741 gctcggactg aacattgtgg aatttgctgg tgatgatgag gcgctgattg acgagcgagt
     7801 taatgcactc tgtgtgcggc ttgatgagct gattgctagc caacaggcag gtgtgattgg
     7861 ctggcaggtg tgccgcgagc tggcgggggt tgaacgtatc tatgcgatgc gcaaaaaagc
     7921 cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca attccgtttg ctgaggatac
     7981 ctgcgtaccg ccggaacatc tggccgatta tattgctgaa tttcgcgcgc tgctcgacag
     8041 ccacggctta agctacggta tgttcggtca cgtcgacgca ggtgtcttgc acgtccgtcc
     8101 ggcactggat atgtgcgatc cgcaacaaga gattttgatg aagcaaatct ctgatgacgt
     8161 ggtggcgctg actgcgaaat acggtggttt gttgtggggc gagcacggca aaggttttcg
     8221 cgctgaatac agcccggcgt ttttcggtga ggaacttttt gcagaactgc gcaaagtgaa
     8281 agcggcattt gacccgcata accgactcaa cccagggaag atttgcccgc cagaaggtct
     8341 cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt acattcgatc ggcagatccc
     8401 cattgcggta cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt
     8461 caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga accggattca
     8521 ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg cgcttgttgg cggatcgcgg
     8581 cgttgaccca ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt tgcggacgtt
     8641 aattgcccgc acgcgcaata gctggcatgc gaataaaggc gaatatgact tctcacacga
     8701 agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagct tgttcgaccc agtgccccat
     8761 caaaattgat gtgccggagt ttcgctctcg ttttctgcag ctctatcaca cccgttattt
     8821 acgcccgctt cgcgaccacc tcgttgctac ggtcgagagc tacgcaccgc tgatggcacg
     8881 cgcgccgaaa acctttaact tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa
     8941 acatatcggc atggttgatt tgccactgct gtcggtcccc tcgctacaac aacaaatggt
     9001 ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa gctctcaatg cagagcagaa
     9061 agcgcgcacg gtgttggtgg tgcaggaccc ctttaccagc tattacgatg cgcaagtggt
     9121 ggcggatttt gtccgtctgg ttgaaaaatt aggtttccag cctgtgttac tgccattttc
     9181 gccaaatggc aaagcccagc atatcaaagg ctttcttaat cgttttgcga agacggcgaa
     9241 aaagacggcg gatttcctca accgtatggc taagcttggg atgccaatgg tgggcgtcga
     9301 tccggcgctg gtactttgtt atcgcgatga atataaactg gcgctgggcg aggaacgtgg
     9361 cgagtttaac gtcttacttg cgaatgaatg gctggcaagc gcacttgagt cacatccagt
     9421 ggctacagtc agcggtgaat catggtattt ctttggtcac tgtaccgagg ttaccgcttt
     9481 gccgggcgcg ccagcacaat gggccgcgat atttgcccgt tttggcgcga aactggaaaa
     9541 tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga catgaagcga aaaaccatga
     9601 aaattcgctc gggatctatg agttatcctg gcatcaggct atgcagcgac tgccgcgtaa
     9661 ccgctgtctg gcgaccggat attcctgccg tagccaggta aaacgggttg aaggcacggg
     9721 ggtacgccat cctgtgcagg ctttactgga gattattaaa tgatatggaa acggaaaatc
     9781 accctggaag cactgaatgc tatgggtgaa ggaaacatgg tggggttcct ggatattcgc
     9841 tttgaacata ttggtgatga cacccttgaa gcgacaatgc cagtagactc gcggacaaag
     9901 cagcctttcg ggttgctgca tggaggagca tccgtggtac tggccgaaag tatcggttcc
     9961 gttgccggtt atttatgtac cgaaggtgag caaaaagtgg ttggtctgga aatcaatgct
    10021 aatcacgtcc gctcggcacg agaagggcgg gtgcgcggca tatgtaaacc gttgcatctc
    10081 ggttcgcgtc accaggtctg gcagattgaa atcttcgatg agaaagggcg tttgtgctgt
    10141 tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt gacgcatatt
    10201 gcttttggtc attatctgca cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc
    10261 gaggatgcta tgtctactca acttgatccc acccagctgg ccattgaatt tttacgccgt
    10321 gaccagagca atctttcacc tgcccaatat ctcaaacgtc tgaaacaact agaacttgaa
    10381 tttgctgacc tgcttactct ctcttctgct gaattaaaag aagaaatcta ctttgcctgg
    10441 cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg acctggatta
    10501 acctccgtcc aggttcgatg accttcttaa tactgcttat ctaccgcctg atattgttca
    10561 aaaataatac taatcgcagt ttgtgttaaa acggcgggtt agctttatga gattattctt
    10621 atcgcccctt caagagctaa gccactgaga gtgccggaga taagcgccgg atggggtaga
    10681 aacccttaag cctgtgtcgc acagacttaa gggttttctt atttctgaac gctgcacttt
    10741 tttacctatc tcttaaatag tcattttcaa tacaactttt tctttttcat cacagcctta
    10801 atgctcctta gataataacc attatctaac aatgagatac ctaattctta gcgtgcctgt
    10861 taacccacac atcaggggct atgcttatta aaagcaggca aaaagcatgt tttacggtaa
    10921 agcccctgcg tttgctgggt tgaactgata atcattatca ctaacatgct gttatacgct
    10981 gaaagcgatg aagtgaggta aatagatgga catgcattca ggaaccttta acccacagga
    11041 tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg atacacatcc gcgagctggt
    11101 ggcaaagcag ccaggtatgg tcggcgtgcg cttaggtgtg aagcaaacgg gctgcgcggg
    11161 ctttggctat gtgctcgaca gtgttagcga gccggacaaa gacgatctgc tgtttgaaca
    11221 cgacggcgcg aagctgtttg tcccgctgca atcgatgccg tttattgatg gcacggaaat
    11281 cgatttcgtt cgtgaaggac ttaatcagat attcaaattt cacaacccta aagcccagaa
    11341 tgaatgtggc tgtggcgaaa gctttagggt ataggcggta ctatgtctcg taatactgaa
    11401 gcaactgacg atgtcaaaac ctggaccggc ggcccgctga attataaaga aggattcttc
    11461 acccagttag ccaccgatga gctggcaaag gggataaacg aagaggtggt gcgcgcaatt
    11521 tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc gtctaaacgc ctatcgcgca
    11581 tggctggaga tggaagaacc gcactggttg aaagcgcact acgacaagct gaattatcag
    11641 gattacagct actactcagc accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa
    11701 cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg
    11761 tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc
    11821 gactctgttt cggttgccac cacttatcgc gaaaaactgg cggagcaggg aattattttc
    11881 tgttcctttg gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata tctcggcacc
    11941 gtggtgccgg ggaatgacaa cttctttgca gcacttaatg cggcggtagc ctctgacggt
    12001 acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac ctattttcgc
    12061 attaacgcgg aaaaaaccgg gcagtttgag cgcacaattc tggtggccga cgaagacagc
    12121 tacgtcagct acattgaagg ctgttccgct ccggtgcgtg acagctatca gttgcacgcg
    12181 gcagtggtgg aagtcatcat ccataaaaac gccgaggtga aatattccac ggtacaaaac
    12241 tggttccctg gcgataacaa caccggcggt attctcaact tcgtcaccaa gcgtgctttg
    12301 tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc agcgattacg
    12361 tggaaatatc ccagctgcat tttgcgcggc gataactcca ttggtgagtt ttactcagtg
    12421 gcgctgacca gcggtcatca gcaagcggat accggcacca agatgatcca catcggtaaa
    12481 aacaccaaat cgaccattat ctcgaaaggg atctctgccg gacatagtca gaacagttat
    12541 cgcggcttag tgaaaatcat gccgacggca accaatgcgc gcaatttcac tcagtgcgac
    12601 tcaatgctga ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat
    12661 aacagcgcac aactggagca cgaggcaacg acatcacgta ttggtgaaga tcaactgttt
    12721 tactgcctgc aacgcgggat cagcgaagaa gacgccatct cgatgattgt taacggtttc
    12781 tgcaaagacg tgttctcaga actgccgctg gaatttgccg ttgaagcaca aaaactcctc
    12841 gccatcagtc ttgaacacag cgtcggataa ggaataaaca tgttaagtat taaagattta
    12901 cacgtcagcg tggaagataa agctatcctg cgtggattaa gcctcgacgt tcgtcccggc
    12961 gaagttcacg ccattatggg gccaaacggt tcgggcaaaa gtactttatc ggcaacgctt
    13021 gccgggcgcg aagattatga agtgacgggc ggcacggttg agttcaaagg caaagatttg
    13081 cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg
    13141 gtggagattc caggcgtcag taaccagttt ttcctgcaaa cggcacttaa tgcagtgcgc
    13201 agctatcgcg gccaggaaac gcacgaccgc tttgattttc aggatttgat ggaagagaaa
    13261 atcgctctcc tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt tggtttttcc
    13321 ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg tgctggaacc ggagttatgc
    13381 attcttgatg agtcggactc cgggctggat attgacgcat taaaagtggt cgccgatggc
    13441 gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg ttacgcacta ccaacgcatt
    13501 ctcgactaca tcaagcctga ttacgttcat gtgctatatc agggacgaat tgtgaaatcc
    13561 ggcgatttca cgttggtcaa acaactggag gagcagggtt atggctggct taccgaacag
    13621 cagtaatgcg ctgcaacagt ggcatcactt gtttgaagct gaagggacaa aacgctcacc
    13681 gcaagcacag cagcatttac aacaattgct gcgtaccgga ctgccgacac gtaaacatga
    13741 aaactggaaa tatacgccgc tggaagggct gaccaatagt cagtttgtca gcattgcggg
    13801 agatatatcc ccacagcagc gtgatgcctt agcgttaacg ttagacgccg tgcggctggt
    13861 gtttgtcgat ggacgttacg tgcccgcgct gagcgatgca attgaaggca gcggatatga
    13921 agtgagcatt aacgacgacc gtcaggggtt acccgacgct attcaggcgg aagtgtttct
    13981 gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg
    14041 gccggcaaag ccattgctgt taatgcatat cacccagggc gtggcaggtg aagaggtgaa
    14101 cactgcccat taccggcatc atctggatct ggcggaaggt gccgaagcaa cggtgatcga
    14161 acattttgtc agccttaatg atgctcgtca ttttaccggg gcacggttca ctatcaatgt
    14221 cgcagcgaac gcccacttgc agcatatcaa gctggcgttt gaaaacccgg tcagtcacca
    14281 ctttgcccat aacgatttgt tgctggctga cgatgccacc gcatttagcc atagtttcct
    14341 gctgggtggc gcagtgttac gacacaacac cagtacgcaa ctcaatggcg aaaacagcac
    14401 gctgcggatc aatagcctgg cgatgccggt gaaaaacgag gtgtgtgata cccgtacctg
    14461 gctggaacac aataaaggtt tttgtaacag ccgacagttg cacaaaacta tcgtcagcga
    14521 caaaggccgc gcggtattta acggtttgat caacgtcgcg cagcacgcca tcaaaacgga
    14581 tggtcagatg accaacaata atctgctgat gggcaaactg gcggaagtgg atacaaaacc
    14641 gcagctggaa atctatgcag atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat
    14701 tgatgatgaa cagatgttct atctgcgctc gcgcgggatc aatcagcagg atgcccagca
    14761 gatgatcatt tacgcctttg ctgccgaact gacggaagca ctgcgtgatg aggggcttaa
    14821 acagcaggtg ctggcccgaa tcggtcaacg gctgccagga ggtgcaagat gactttttcc
    14881 gtcgacaaag tgcgggccga ctttccggtg ctttcgcgtg aggtaaacgg tttgccgctg
    14941 gcttatctcg acagcgccgc cagtgcacag aaaccgagcc aggtgattga cgccgaggcc
    15001 gagttttatc gtcatggcta cgcggcggtg catcgcggta ttcatacctt aagcgcccag
    15061 gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg
    15121 gcggaagagc tggtgttcgt ccgcggcacg acggaaggga tcaatctggt cgccaatagc
    15181 tggggcaaca gcaatgtgcg ggcgggcgat aacatcatca tcagtcagat ggagcaccac
    15241 gctaacattg tcccctggca gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc
    15301 cctctcaacc ccgacggtac gctgcaactg gagacgctgc ctacgctgtt tgatgagaaa
    15361 actcgcctgc tggcaattac tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg
    15421 gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg
    15481 gtgatgcatc atccggtgga tgttcaggcg cagggttgcg acttttacgt gttctccggg
    15541 cataaactgt atggccccac cggaattggc attctttatg tgaaagaagc cttgttgcag
    15601 gagatgccgc cgtgggaagg gggcggttct atgatcgcca ccgtcagcct gagtgaaggc
    15661 actacctgga ccaaagcacc atggcggttt gaagccggta cacccaatac cgggggcatc
    15721 attggtcttg gcgcggcgct ggagtatgtt tcggcgctgg ggcttaatag catagccgag
    15781 tatgaacaga atctgatgca ttatgcgcta tcacagctgg aatctgtacc ggatctcact
    15841 ctctatggcc aacaaaacag gcttggcgtt attgctttta atctcggtaa acaccacgcc
    15901 tatgatgttg gcagttttct cgataattac ggcattgctg tgcgtaccgg acatcactgc
    15961 gctatgccat taatggccta ttacaacgtc cctgcgatgt gtcgggcgtc gctggccatg
    16021 tataacaccc atgaagaagt ggatcgtctg gtgaccggcc tgcaacgtat tcaccgtctg
    16081 ctgggataac agggaggcac tatggcttta ttgccggata aagaaaaatt gctgcgtaat
    16141 tttttacgct gcgccaactg ggaagagaaa tatctctaca ttattgagct gggccagcgt
    16201 ctgccagaat tacgcgccga agacggaagc ccacaaaata gcattcaggg ctgccagagt
    16261 caggtgtgga ttgtcatgcg ccagaatgcg cagggaatta ttgaattgca gggcgacagc
    16321 gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta ttctctacga tcagatgacg
    16381 ccgcaggata ttgtcaattt cgatgtgcgt ccgtggtttg aaaaaatggc gctcacccaa
    16441 catctcaccc catctcgttc acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa
    16501 gccgctgcac ttagctaaac taaagggaca gctttcatcc ggttggctca cattgccgga
    16561 tggtcaatgt ttacactgac ctgcacagga gtgttttggt ttcaggtgaa cataagcacc
    16621 atccgacgtt tcagcgtgag tctccggcaa atacaggagg tttacaatga aacgcgcgtc
    16681 tttgcttaca ctcacgctta tcggcgcttt tagcgccatc caggctgcct gggcggttga
    16741 ttatccgcta ccaccaaccg gaagccgact ggttgggcaa aatcaaacgt atacggtgca
    16801 agaaggggat aaaaaccttc aggctattgc ccgacgtttt gatactgcgg caatgttgat
    16861 ccttgaagcc aataacacta tcgccccggt gccaaaacct ggtacgacga taactattcc
    16921 ttcacaactg ttattacctg atgcaccgcg tcaggggatt atcgttaacc ttgcagagct
    16981 gcgcctttat tattatccgc cgggagaaaa tattgtgcag gtttatccaa taggtattgg
    17041 attgcagggg ctggaaacgc cggtgatgga aacgcgtgtt gggcagaaaa tccctaaccc
    17101 aacctggacg cctacggcag gcattcgtca gcgttcgctg gagcgtggca ttaaattacc
    17161 gccagtcgtt cctgccggac caaataaccc gctaggacgt tacgcactgc gcctcgcgca
    17221 tggtaatggc gaatacctca ttcatggcac cagtgcgccg gacagcgttg gtttgcgcgt
    17281 cagttcaggg tgtattcgca tgaatgcacc ggatattaaa gccttgttct ccagcgtgcg
    17341 gacgggaacg ccggtgaaag tgatcaacga accggtgaaa tattccgtgg aacctaacgg
    17401 gatgcgttat gttgaagtac atcgaccact atcagcagaa gaacagcaaa acgttcagac
    17461 aatgccatac acactgccag caggctttac gcaatttaaa gacaataagg ctgtagatca
    17521 gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat ccggtttcgg tgagcagtgg
    17581 agcaactccc gcagccagca atgcgccttc ag
//
LOCUS       DAJTYI010000077        17510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYI010000077 DAJTYI010000000
VERSION     DAJTYI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(29..1270)
                     /locus_tag="OO564_002518"
     CDS             complement(29..1270)
                     /locus_tag="OO564_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6653602.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(1270..1452)
                     /locus_tag="OO564_002519"
     CDS             complement(1270..1452)
                     /locus_tag="OO564_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653603.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(1464..2960)
                     /locus_tag="OO564_002520"
                     /pseudo
     CDS             complement(1464..2960)
                     /locus_tag="OO564_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3194..3688)
                     /locus_tag="OO564_002521"
     CDS             complement(3194..3688)
                     /locus_tag="OO564_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6653604.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3814..4164)
                     /locus_tag="OO564_002522"
     CDS             complement(3814..4164)
                     /locus_tag="OO564_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6653605.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            complement(4215..4547)
                     /locus_tag="OO564_002523"
     CDS             complement(4215..4547)
                     /locus_tag="OO564_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653606.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            complement(5010..5402)
                     /locus_tag="OO564_002524"
     CDS             complement(5010..5402)
                     /locus_tag="OO564_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR6653607.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            complement(5386..5862)
                     /locus_tag="OO564_002525"
     CDS             complement(5386..5862)
                     /locus_tag="OO564_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6653608.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            complement(5866..6192)
                     /locus_tag="OO564_002526"
     CDS             complement(5866..6192)
                     /locus_tag="OO564_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6653609.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            6489..7820
                     /locus_tag="OO564_002527"
     CDS             6489..7820
                     /locus_tag="OO564_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR6653610.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            complement(7849..8217)
                     /locus_tag="OO564_002528"
     CDS             complement(7849..8217)
                     /locus_tag="OO564_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR6653611.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(8232..9221)
                     /locus_tag="OO564_002529"
     CDS             complement(8232..9221)
                     /locus_tag="OO564_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6653612.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(9229..10026)
                     /locus_tag="OO564_002530"
     CDS             complement(9229..10026)
                     /locus_tag="OO564_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6653613.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(10046..10435)
                     /locus_tag="OO564_002531"
     CDS             complement(10046..10435)
                     /locus_tag="OO564_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6653614.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            complement(10432..10758)
                     /locus_tag="OO564_002532"
     CDS             complement(10432..10758)
                     /locus_tag="OO564_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6653615.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(10755..11408)
                     /locus_tag="OO564_002533"
     CDS             complement(10755..11408)
                     /locus_tag="OO564_002533"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6653616.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(11408..11902)
                     /locus_tag="OO564_002534"
     CDS             complement(11408..11902)
                     /locus_tag="OO564_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6653617.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11899..12840)
                     /locus_tag="OO564_002535"
     CDS             complement(11899..12840)
                     /locus_tag="OO564_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6653618.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(12830..13009)
                     /locus_tag="OO564_002536"
     CDS             complement(12830..13009)
                     /locus_tag="OO564_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6653619.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(13185..13736)
                     /locus_tag="OO564_002537"
     CDS             complement(13185..13736)
                     /locus_tag="OO564_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653620.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(13780..13980)
                     /locus_tag="OO564_002538"
     CDS             complement(13780..13980)
                     /locus_tag="OO564_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6653621.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            14071..14745
                     /locus_tag="OO564_002539"
     CDS             14071..14745
                     /locus_tag="OO564_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR6653622.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            14918..15214
                     /locus_tag="OO564_002540"
     CDS             14918..15214
                     /locus_tag="OO564_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653623.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            15490..15800
                     /locus_tag="OO564_002541"
                     /pseudo
     CDS             15490..15800
                     /locus_tag="OO564_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15797..16177
                     /locus_tag="OO564_002542"
     CDS             15797..16177
                     /locus_tag="OO564_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653624.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            16243..17067
                     /locus_tag="OO564_002543"
     CDS             16243..17067
                     /locus_tag="OO564_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCR6653625.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GRADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            17195..>17510
                     /gene="yfdR"
                     /locus_tag="OO564_002544"
     CDS             17195..>17510
                     /gene="yfdR"
                     /locus_tag="OO564_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6653626.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKY"
BASE COUNT         4484 a         4591 c         3997 g         4438 t
ORIGIN      
        1 actgacagat ttcagactca gcggtacatc aagacgttgt ttcgtctgca ttggcgttat
       61 ccttctgctt accggcttta ctgccatcgg agggtttcgt ggtcatgttc atcggtgtga
      121 gatagacatc accaccggga cgcggattca tatcttccag gtcgcggcag tcattgggag
      181 agtaaattcc ccagttaatc ccggtggcgt aggcttcaaa acgggacttc atatccccgc
      241 gcagtaacgc cccggcgtta aatttggcgt aataaacgcc ctgcttactt tttcgtacca
      301 gtccggtgtt gatccgctgt tcgatgcggg tcagatacgg caccagtgaa tagttgataa
      361 atcccagccc cagctcttca atattgttga aagtggcgcg atcggtgttc tgcaccatgt
      421 gcaacggcac ccggaacaga cgacagattt cttcaagctg aaacttgcgg gtttccagga
      481 actggctgtc ctcggcgttc agcgccatcg acttccagtc cagccccatc tcaaggatca
      541 tcgggcggtg agcattgcca agcccggtgt gacgctcctc aaaatctttc ttcaggcgct
      601 cgtaagcctg atctgacagc gtctgctctg tacgcaacac acccgacgtc accgcgccat
      661 tgctgaacag tctggccccg tgctcttcgg tcgctgccgc cagcgatatt gcctcgcggg
      721 cataggcgat gggattcagc cccaccagtc cgtccagcgt cagcgtgcgc acatgccaga
      781 tatcctcctg gctcagtaca tccgcggagc catccgggaa tgtgacctga tagaccggct
      841 cccagctact gttaagcttc ggtaccacac agctgggatc gacgggcagc agttcagcca
      901 cttcgccaaa tgctttcact ttgtaggcgt aaaagtttcc ccgcaggcac agacaggtga
      961 ccaccagctc ccagaactcc tgcggcgtca tatagccatt gggatgcgtg gagatcagct
     1021 tatgcagacg ttcgccagtg gctctctgct tcaggctgcc gttcaggtga tacaggttgc
     1081 agggcaacat cccgaccgac tccgccagca ccctgacaca ggaaaaaacc gccgtcagtc
     1141 gcatggcccg ctggctgctg atctgctttc cggtataggt gtcgtaggac aacccgatat
     1201 catccgccag ctctgctggc gtggtcaccg gtgcgtcact ttttcgttga aataatcccg
     1261 aaaagaacac tatttacctc cgccgacaga cgactgtgta cggtcgagat atcgcgccac
     1321 cagccacgac cagaacaggc acaacgcccc ggcaacaaca aaccccgccg ggggataaat
     1381 cagccaggca ccatacgcca gcaaaagcgc ccccagcacg cccaccagag gcgcgagaat
     1441 cagcatgatc ataattacct cagttaaagc gagcggatcc cataggactc aatgtggtca
     1501 gacagcgtgt cttctttctc gtacagcatg gctctgccaa ccgccataat cagcgcaact
     1561 gcaccgtcaa ttttgttttc cgcctgctct ttgacgggct tcaccacatc atcgttaccc
     1621 ggaatggttt tgccgaccac gttgccgata cactaggtca tgatgggatt gccatcatga
     1681 tgaaagcgcc ccgattcaat tgccgcttcc agctctttca tcgggtcgga catgttggtg
     1741 tagttctgaa tgatagtgat ggggttcagg tcttcatcag caaggtcatg tgacaacccg
     1801 gtcgccccga aggggtcgat gggtgactca ctgaccggac tgattttgtt cgccgctttg
     1861 gcctcctcga ggatgtagcg ataatccacc tccgcaccat cggtaacggt cagaacgccc
     1921 atttccaccc atttctgaaa gcgttcggct gtccgtcgat cttcattttt ctcgacgctg
     1981 tacaccgtgt catacggtac ccagaaacgc ggggccacac tgtagtaatg cgttttaccg
     2041 tcaatctcgc gggtataaag tcgcgccatg ctgttcatat ccagcttacg cgccaggtca
     2101 aaggccagaa tgcacggctg cccctcgaac tgctcaaggg tcagtgattt atcctcgcag
     2161 ctctgccagc tcaccaggtt gaaatacgcc gaacgcgccg acacccagat attgaggtgt
     2221 tttgttttaa agacgtttgc cagacgggcg ttatttttcg cacgctgctg ctgacttaac
     2281 aaaaattcgc gataaaccga cacgccaata tttggattgg ctttttccag cacctgcggg
     2341 tcggtccagt cgtcaccttc atcaacggta tagataatcc cgaacagttc atcgttgggt
     2401 accgaaccgt tgagcatctc gatgacttcc cgccgtttgt cgtagcacgg cccctcaatg
     2461 ttgtacccgg cggtagtgat agcccacatc agtggctgac gtcgcgcccc catcccggta
     2521 agcatcgtgg tgtaaagcgc atctgtggcg tgctcgtgat attcatccac caccgcacag
     2581 tggggtgatg aaccatcacc ggggttacca atcagcggtt caaaccgcgc cccatcctcc
     2641 ggacggttca tgtttgaggc gttaacctca atcccgaacg cttccgtcag catgggtgtg
     2701 cgtttacaca tcagtcgcgc cgggcgaaag acttcccacg tctgtttctc tgtcgtggca
     2761 ccggaataca cttccgcgcc aaactcgtta tcacaggcaa aacaatacag ggcaacaccg
     2821 gcagagattg ccgatttgcc gttcttacgg gggatttcgg tatacacctc ccggaagcgg
     2881 cgcagccggg accctttatt gacccagcca aacgcacagc agatcacaaa gagctgccac
     2941 ggctccagcg tgatgggcat cctcttgaat gcccactccc ccttggtgtg tggcaacagc
     3001 tgaataaatt tcgcggcccg ttcagccagc tccttgtcga agcggtaacg aaacgactta
     3061 cttttttccg ccatcaggtc atcaagatgg cgctggcagg cctgaatcac aaactggcag
     3121 gccacaatct ttccgcgcac gacatcacgg gcatactgat tggcagcatt tacgttgggg
     3181 taagatttcc ggctcatgac tcgatgattt tcagaaacgg gttagtggct ttcttctgcc
     3241 ccgccaggcc aatcagacgc tggcggctac tggggtcgag tccgagcatt gcccccgtgc
     3301 tgctcatctc ggactcctgt tcttttttgg cggtcagctc cggatttttg accataccgc
     3361 ccattgcacc ggtgatggtg ttgccctgtc tggcaatatt tttcacggca cgccgccaga
     3421 actcataggc cacacaccac cgctcaagca ccgcaaggtc agtcacgcac agcaggccct
     3481 gaccgcagag ttctttggtt gtcagttgcc acatgatcgt ggcgagagga agctcttctt
     3541 cagcgaacca ctccggtggc tcaacacctt tgatgggcgt aaaaacaggt tcatctttgt
     3601 tcagggctcg cttgccgggg tttccggcca gcgccttgcg cgccgttggc ttagggcgac
     3661 gcccggaacg ccccgccgtt ccagccatat gcggcactcc tggttaaatt tcatttttcg
     3721 cgggtataaa aaaacgatgg ggcgggcagt ccggaagacg tcaggtcaca gagatttgac
     3781 ccgcccctcc cctcagacag ttgagaatta tgatcacttt agccgttcac gggccgtctt
     3841 ctccttatgg cacggccagc acaggctctg cagattgctg tcggcatctg tgccgccatg
     3901 cgctttaggg atgatgtggt caacagtttt cgcctcacgc accacaccag cacgcagaca
     3961 tagctgacac aggcctttgt cacgcttcag gacacgcacg cggataacat cccacttcga
     4021 accataaccg cgctgatgac gagattgtcc tggcttgtat tgcttccagc cttcgctttt
     4081 atggctttcg cagtagcctg acgggtccgt ggttgtagag cggcagccgc gaacacggca
     4141 ggcttttggg attctagggg gcatataaaa aatttataat aagtaaaata ataaagaaaa
     4201 atactcaaag gcaattatct tgactcaaat acaaatctag aagagatgag cttatcaagc
     4261 attttcataa gtaaatcctt actctcacca ccatttatat atttattaaa gttagactcg
     4321 aactcatgcc aaactttttc taactcagta ttatcaaata agtgctctgt cgcaaaccat
     4381 gaagactggc aacaatcgaa aatactcaaa agttcatcaa atcttttaac attctcttct
     4441 cccaactcaa tttgctgact aataagatct acatctttaa atagccattt cgcaataact
     4501 tgctctcgtt catcattgag tttctcgggg tcaatattga caggcataaa taatagagct
     4561 gtctttaatt tctttaatgc attcctaaaa tctattttta cttttgtttt ctcttgttcc
     4621 cgccacgtaa acaatgcctt gaacgccaaa accaaagtaa caatagtagc tccagcactc
     4681 acccaagatg caatcattgc ccaacaagcc cactcagcag cagcacggtt tgctacaagt
     4741 gtctcatatg caatataatt ttcgttcatt tttacctcac tgttaatagt gagagtattg
     4801 taactaaatg cataataact agaacagtca aataataata tttcacctaa tgaatattat
     4861 tattcaaatg cagattgtac ttttgctctc ctaacctacg aagatcagac ttatcacggt
     4921 tacatagccc cagtgctgat agcagactta cattcaaact aagactatcc ccataagtca
     4981 gaggattggg tataactggc tgtggagttt cagcgagcag gttcgtcggt aacggcatcg
     5041 ttggaacctg cacgtatact gtccgcgtac ttccgcaacc ggtcagcagc gacatcaggc
     5101 acagggcgtg aagcacaatc atcatccgca acagccactt tgatatcttc ccgggctctc
     5161 tgtgactcca gtgcgatctg ctgttttgca tgctggttag cctccagaac tgtattgacg
     5221 atttgtagtg attgcaggac gttattggta atggcagttg ctgattcagc atttcgtaca
     5281 gcctcatcag cacgtttctt ttcgtactga tatttgctgt agtagtggtt ggccgaccag
     5341 atgaaagaac caatgacagt aacgaagaaa ccaacgataa gcagcttata gctcagcttc
     5401 atttaccacc ccaccagcct ctttaaaccg ggcaatcaaa tcaccgattt tatgttcata
     5461 ctgaccataa cctgcaccgg gtaacgacgc ccagatattg ctgcaacggt cgatagcctg
     5521 acgaatatca ccgcgatcaa tcatcggtaa agcgccacgc tctttaatct gttgcagtgc
     5581 cacagcatcc tggcttttgg gggagaagtc tttcagagca agctgcttac ggtaggcatc
     5641 ccaccagcgt gaaagaagct gataacgtcc ggcggctgtt gatttgagtt tggggtttag
     5701 cgtgacaagt ttgcgagggt gatcagagta atcagtaaac agttcgccgc caacaataac
     5761 atcataaccg tgattacgtg tcggttgtcg cccgttatcc gttccttctg accatgccac
     5821 catatcgagg aaagctttac gctgggaatt tagtacctgc ataaattact ccttagagcc
     5881 accaaactta ttaccgatta ctctcattgc agccccacga atagcatcga ccccgattag
     5941 cccaacgccg ccaccaatgg caacagaaag agatttaggc catccgacat actcaagagc
     6001 ggatgcaaaa gtcagcgtca gagcaccaca gagcaaaatc tcgagcgttt ttcgtttcca
     6061 gcccccacca ccgccaaaat atgcaatgcg caagccagcc ataacaatcg acataatcac
     6121 tgcgcccagc ggtgtgtctc cacgccacca gctctggacc aactccaacc aggtatttgg
     6181 gttatgaggc atttgtagtt atctctcacc tcgctgatac agcaggtgca aattgaggga
     6241 acatcatgta ccgcaaatca gaagcggaaa cgtcaaagag ccgaaccaat ggataactgc
     6301 gggataggcc aggcccaacg aatccccagc aacaaaaacg acaaaacccg ctcgacggcg
     6361 ggtttaagct gtgtggcgaa gtaaccactc ttaacagatt acaagaattt ttgcggaccg
     6421 cgttaatgat ttttaatccc aaagtcgtat tattcgtttt ttactgtagg agttaatagg
     6481 tttatattat gttagataca ctatctttca ctgaacgtga cgagttccaa cgcagaaaca
     6541 tcgctgaaaa tatcatcaag ttgctaaaac cagaggcaga catttcacca ctggtaatag
     6601 acggcgcatg gggaacaggg aaaacagaat ttagtatcaa actgaaaaat ctcattattg
     6661 agcaagaaac tgaatctaaa gttgtttata ttgatgcctt taaaggggat catgcagaat
     6721 ctccattact tctcataacc tctgcaattg ccagcatttt acctgaggaa gaaaaacaac
     6781 acttcattaa gagatctctt cctgcaattc gctttggttt aaaaacggta ctaaaagctg
     6841 gtgcaggttg gtttttacga caggaggcta gtgaagtttc cgaagaattc caagatgcga
     6901 tgaagaaagc aagcaatgca gcaatagatg ggactattga aaatttgctt gaagaccata
     6961 tggaatcaga gaaaaacata aattcactta aatcttgcat cgaaagcatt tcagaaagtc
     7021 aaaagatagt aattataatt gatgaattag accgatgtaa gccgagtttt tcaacaaatg
     7081 tcattgaaac aataaaacac atttttgaca tcaataatgt cttttttatt ttagttacaa
     7141 acacagaaca attaaaagca tcgataaatc atatttatgg ttatagtatt aactcacaaa
     7201 agtatcttga caagtttata aaatatacca tcacgcttcc agacacatgt ttaataaatg
     7261 gtcacgtcgt atgtaaaaca tctgttatat attggaatta ccttgtagac aagacaaaat
     7321 cattaaatga aattaatagg ttatctggta gatttatccg tgatttaatt caacgaacca
     7381 acttatcctt acgtgaaaca caaacttttt cacgcaatct taacattttt caactattaa
     7441 acgacgatga gtacaaaagc agtgatcctt taataaataa gatctttgtc gtagctgtct
     7501 tcatacattg ctttggtgac aaggaaaaac taaaacaaga aattaccgca gaatctatat
     7561 ctcatttagc agatctgctt aacataaaag aaatacctta ttcttatgag agaagatcgc
     7621 aaattcctga aatatctatt gtattcttcg gaataattaa agacagtatt actcttaatg
     7681 agcgatttgc ccctaaaagt gatgaagaac ttaaaaaatt caccactgtt tatactgatt
     7741 atgaacacct aaatttttgg agtactacac ccagagagtt attgataaaa tatattaatc
     7801 aaatgtcatt catccagtaa ataatacgcc cctgcatggg gcgtattatt aaacgtccaa
     7861 atctaatgtt agatctagca tggaaaggca gccatcaata aatccttcgg ctagctgtat
     7921 ttctatacgt atcaatttct catcctttcc acgcgccttt gcaatcttac gcttggatat
     7981 tctgtataaa taatgtgcca caagaagcga atgctcataa ggtctttttt gctttagacg
     8041 agcaagacaa ccttcaataa ttaatgcatc actatccgaa caagccaagc gtttcttgct
     8101 ggtataggga agaagtccct taaacccagc agctataggt gaatagtcaa cgcctgaact
     8161 atcactcgcc gcccatgctc cccaacgctc aagaaccatc tgaatatcac gcatcaactt
     8221 tctccaccaa atcaggccag cacgccaatt gccagcgcac gatcgataaa acgaaatatc
     8281 agctccagct gggagccata cttctcttca aatgccacgg tatccgcatg cagctcgtcg
     8341 tgatgctttc tgcacaaagg caacacaaag aggtcatgcg cttttgtacc cattcccccc
     8401 tgaccgtggc ctatcaggtg gtggggatca tcagcgggct ttccacaaca tgcacacggc
     8461 tgtgtcttaa cccagcgcgt gtacttctca ttaacccagc ggcgacgttt tgggcgtaac
     8521 ataaaagact ccggcgactc cggatccact ttcagcgcca gcaccttttt tgccttatct
     8581 tggatgatgc tggtggcagg aaccgaaggc acaaggtcac tttcccgggt gacagacggc
     8641 acaacaggct ttggtaatct cagtgcctta cgggctgcac tttccggtaa ggcatccgcc
     8701 aggtcattac gaatcagcca ccagcacagt tccggcattg tcacaacgtg actatcatca
     8761 aaaccgagat cccgacgcac aacagacaac acccagcggg cacagttatc cgttgccatt
     8821 gattccagcc gttccgtgaa ctgatcgcgc agctggttat cacagtgcca gcacagacgg
     8881 attgcgcccg gagcgtgtcg cattgtggtc atgttctcgc tgtgccagtc ggaatgaggc
     8941 cactggcagc ctttttcacg aagtagccag ctttcaagac attccacgcc accagcacga
     9001 cggatcactg cctcattgcg gaacacggcc cgaacagcag gatcatccgc cagcggttgt
     9061 gatgccgcgg gaacggcacc actggcgaaa gatgaataac gttccggctc aggctccagc
     9121 agtacacgcc cctgcataaa caggggcatc agctctgaac ctggtctgaa caatacgatc
     9181 cccatacgcg gggcaatttc aggggtcagt agtgctctca cggtcacctc aatgaacggt
     9241 atcgagcagc tttaacagct cagggaatcg ggattcgaag aaatgcggct gcgtctcgcg
     9301 cggatttgca ggactggtaa tgttcttgcc gaacatgcag cctttcgccg tcagcgacca
     9361 gaattttttg atgttgttaa tcgcggtacg gctgtatcgt tcgcgttgtt caacgatccc
     9421 cagcttcgcc atctggtgat atgcctgatt agctgtcagg cggataccat actgtttcag
     9481 cagtgcactc agcgacagcg tagggcgact tgagccatca ggcgcgtcag caggagcatc
     9541 aatggcatag cgtggtgcca gattcggtaa gccaacagcc tcctggagtt tctgacaggc
     9601 cccaagcaca gatgagttag acaggtttaa ctcccgccgc ataaagtcca gcagaatcac
     9661 gccagcctgc atcttgtcag cagcctgccc ggataatttt tccggtgcgc tggttaccat
     9721 atcgaaagta cggatcacct tcagatggaa tgacgggctg atccacattg cataggcata
     9781 caccagttct ttgcagacat acgtcccctg gttatttccg ccacgactaa cgttaactgg
     9841 ctctatattg accgagttgc aaatctgcaa ctcgcttatt aaacgttcgg tttgctcatt
     9901 gcggagccag aatgcaggct tatgcttatc ctgagaaccg gcagccctgt gcagatcgtt
     9961 caggctgtaa cgcccataag catcacgacg aacttcaata ccatcaatga ccatcagatt
    10021 attcatactt cgtttctcct cttaatcagg cggctgcacc cgccgttttc tcgtacttat
    10081 tgatagtgat ctcgaccttc ccttccggga taaccggccc ccactccacc agcattcttt
    10141 tcacctgact gtcgtcttcc cacacacccg cgtgggtcag ggcgtcaaac agcgccttgt
    10201 tatagttgtc cagatcgcgg atccggttat ccggaggaaa caacacgatc tccactgaag
    10261 caggtgccga cgttggtttc ggcagacgac gtaactgctc aactattgct gcgcacgccg
    10321 cgctctggaa ttttcgcccc gccgcgctta tcaggctctt accagcaaac gcccctttgt
    10381 tggggtgtcg ccagtacgtg ttcacgctgg gcggaaaagg caggatcagc ttcatacttt
    10441 caggtccctc tcatgtaacc agtgggttgc acgcagcctt gcgttttcct caccggcaag
    10501 cagtgcgcgg ataatcccga ccgcctcgct gtcgtcgtcc ttcaccgcgg tatgaagcgt
    10561 tatcccccgg gccacgccac gctttatcgt gatgacgcct tttttctcca gtgcgcgaag
    10621 atgctccacc gctgcattca ccgaacggta tcccagcatg gttgccacct cctgattggt
    10681 tggcgggaag ccacgttctt tctgataaga aatcagcata tccagcacct gctgctggca
    10741 ttgagttaac gtcgtcatgc cgccatctcc ctgaccagtt tttccgcctg ctggcgaacc
    10801 tgcgccagaa acgcctcacc acatgcctca agttcatcgc gcccgatgta gctgattgcc
    10861 ggtcccttcc aggtcttgtc aaaaacagca atagcaccag cgaaaaaagc tcctgtcagt
    10921 acctgcttct cgtctttcgg gataaaccag acaggcagtt caaaaccaat acgcccgcga
    10981 ataaaagcaa tatgatctgc atcttccggc caccacactt cgctggtggc agctttgatc
    11041 aggaaaacat agcgcccgcc cttatcacgc atggcactgg catgtttcat gatgtaacgc
    11101 atgccggtga tgtattgccc ctcatgctga ctggcgcggc tgtatggggg attaccaaag
    11161 gcagcacctt taagctccgc aagacgttct gaccagtcat gcgccagcgc gttgtcttcc
    11221 gccgtgtaat acgcggcaca tttggcgtta tcaccgtcag tgaacagatc cagaacaaac
    11281 gggccaaaca gggtgttaat tccccagaaa atgttgtccg gcgtgcgcca ctgatcgccc
    11341 acttccttca gttcatgggc tggtttgttc cgcagttcca ccagcgcctg gcaatattta
    11401 ttactcatta agcccccacg taattccctg acagatacca ctcttcaccc gatgcagcgc
    11461 gcttgctgct tttccgtaag caccgctcac gacgcgccag aaaattgttt cgttctggct
    11521 gggagtggct ttcacggaat gccgccatcc acacggttgc agcacgacgg tataagcccc
    11581 tggactccag ttcttccgcc tggcgggtca ggcacaaaat cacacgggga tcgttagtgc
    11641 cgacatagaa attgcgcaca ggtctggttt cacgaactgg ttgtggttcc ggttcctgcg
    11701 ctctctcagt caggcgcggg aaatgtctgc gtgtatctcc ttcacaacgg tgagccacac
    11761 gcccactctg acgtaacttg cttgctgact gcagaacgcg ctgccgtgag taaccggcaa
    11821 aagcatccgc aatatctccg gaagtacagc cccgatgggc ttcaatgaat ttctgaacgt
    11881 cattcaaaag actcatgctc accccctgaa tcctgccggg atttggctgt agtccacgtt
    11941 gtcgtaacta gctttgaagt acgggtcttc gcgtttttcg gtgtacgtgc tgacggacgg
    12001 cgataagcgc agggaaagct catcccattt ttcccgcagc ttcgacgggc tgagcacgtt
    12061 acggcaccag aacggatcgc gactgacgcg gctgtacatc tcgcagattt gtttatgagt
    12121 acgaccatcc tgcacacaca tcaggcgaat ttcgtttgcc caggctgtcc agttcggttc
    12181 tttgggacga accacctcgc cgtcacattc ggcggcctgc tcgtacaggg cgatgatttt
    12241 tttccagagc cactgtgcgc aggtcaaatc atcctgcgtt ccccactggc gctttttagg
    12301 gctgaataca accgcatcag gatggcgagt taaaaactcc tgttcagccg tctgcgtgtc
    12361 cggttgcgaa gcgtccggac gagaagtttt tttatctgac ggatcatgtt ttgattttac
    12421 tgacggatcc ccgccagatt ctgacgggtg aaaacccgct tttttgccag atttcgacgc
    12481 atcaaatttt gacgggtcag attttgatgc gtcagatttt gacgggtcag aatctgacag
    12541 ttgagaaaat gccgctgcct gaagcttcgc aacgttaagc tgataaacat tcgacgcatt
    12601 gcggttaccc tggcgacgcg ccttacgcgt taaccagcct tctgcttcca gccgtgcgat
    12661 agccgttctg acggtactca ttcccgcgcc aatctggcgg gcaatggttt caattgatgg
    12721 ccagcacaca ccttcgtcat tactgaaatc agccaggcgg gccataattg ccacgctgga
    12781 taatttcatg cctgatgcag cgcaaccatc ccatacatag ccggttaatt tagtgctcat
    12841 gaccgacctc tatttccctg aatttacgac gaaactgttc gagcgggctg aagcactcat
    12901 gctcatagcc ttcgcggagg tagataacac gttgtgtttc cggctcccaa cgaatgactc
    12961 tgacgggcac tccgtagtgc tctttgaacc agcggttaac ttgtcgcaaa ggactgtctc
    13021 cttctgccgg ttgaaatcac ccacagccca ctctgcaaag ctgtgggtta caatttccct
    13081 gtcacctggt acatttactg catagcaata ctccaccttc gcttttccac ccggtacagg
    13141 aagcgcaatc agttgcgagc gacggtagtg tgttgttaaa ctgttcatgc gttagtttct
    13201 ccacagtcac gacatgccac ggcgcccgga gctgcacact cgcgggcgtc actactttct
    13261 gaaacgcaaa agattttgta gaccagtgct gcatgctcct gcagcttcga aattgagagg
    13321 tactgctcat cgttaattgc tgtcttctca tgcggttcca ctacaccgtc ttcaattgct
    13381 gaacgaatct gttttgaata actgccgatc tgttcaatga cttccagcag gcgttggttg
    13441 atatcggcgt tgtccacatc ctcgacgtca ggaagagaca caaagacgcc atttgcagac
    13501 tgcgccacag catcagcaat gaagtgagtg ccaccagcac gctgtaaaac cattgcccat
    13561 cccagcggga aaatctgatc gccatctgca cgaaggcggt tgaataaagc gttttctgtt
    13621 acatcgagcc agtcagccgc ttcagcgtaa ccacccggca acgccgcgat agtttttctg
    13681 acagctttca cgtaccactc aggctgtttt tctattttcc agtgatgctt acccacggtt
    13741 agcctcatcg ttctgtggtt aaaaattgaa agtgttctgc taatctttcg gatagatatc
    13801 cggtcttaag tcagatttcg taattgcacc tgacgtgcat tgctcaagtt ttttagccag
    13861 cacaaaactg gcttttttat agccattgaa aaccagccgt aagtagccag gtgttgagcc
    13921 aacttttccg gccaactcgc cctgctgttc tttggttaaa gagtcccaat acgctttcat
    13981 acaatatgta cctccggtgt acatgttaca tgattgaaat gaacctccaa gacacttgta
    14041 ccttaacggt acaagggttt taatttcgtt atgaaaacaa tccatgacat ccggcggtct
    14101 aacgccagaa aactgagaga tggtgttggc gggaattctt cctttgccac tatgattgat
    14161 cgcgagccaa cccagaccag caggtttatg ggagatggtg ctactaaaaa tatcggtgac
    14221 agcatggcac gacacatcga aaaatgtttc gacctgcctg tcggatggct cgatcaagaa
    14281 caccagacaa cgaacatcac aaaaaaacct gatgtttcaa tcactaataa acaaatcaca
    14341 ttagtccctg tcatatcatg ggtacaggcc ggagcatgga aagaagttgg atattctgag
    14401 gttgatttga gcacagcaga aacgtatccc tgccctgtac cctgtgggga aatgacttat
    14461 atcttgcggg tgataggtga ttcaatgatt gatgagtacc gcccgggaga catgattttt
    14521 gtcgatcctg aagtacctgc ctgccacggt gacgacgtta ttgcattgat gcacgataca
    14581 ggcgaaacca ccttcaaaag gttgatagaa gatgggacac agcgttatct caaagcgtta
    14641 aacccaaact ggcctgagcc ttacattaag atcaacggta attgctctat aattggtaca
    14701 gtgattttct caggaaaacc aagaagatac aaaatcaaag cctaatcaat gtttatgaac
    14761 ctgcttcggc aggttttttt atacttgaca atgtaccctt ggaatacata atgtacccaa
    14821 gcgaaacaac gaacaggcag gacgcccacg aagtagccgc ctggggcata tgaagtccag
    14881 gatgattcgt tgagtcatgt tgtgccacta ggcactcatg ttaaagcagg tgtatgaaat
    14941 gaaagtccag attttaaaca ataactgtga agtcgtttgg tcatacgaca tagccgcccc
    15001 tgtagatcag agcggcgata gctggaccaa tgggaaacat cagattatgg ctggagttgt
    15061 gttctcttta cgccgtgctt tggaacaggc tgaagtattt ccatcagacc ctgaatggaa
    15121 atggcctttt tctatttgtc caaattcgga gagcacattt cagaaaattg gtcagaaagt
    15181 cgcactcgaa gagcatcagc caactgtttc ctgatttttt caggtaactc gtcggcatcg
    15241 cagaaacaac aacgctcgat catgttgaaa gccgattcgt agaactgttt ctgctgagtg
    15301 tcgctgagac aggaaaagag cgacgttacg atgattttat taattgcatt atcaagttct
    15361 ttttcatcaa aagtcatttg attttccttt tatgtatacg ggcttaaaag gataccaccg
    15421 agcctgaagt ggtgaaaaga caggcacata acagctaagt attttcaacc aaagagaatc
    15481 cttagagttg tggtgaatgc ggctcagcgc acgcgggtta aggttgaggc tgacagtcga
    15541 ccttctgtgg atacccaccc gtctggtgtg caaccttcgc caggcaccgg gaggcacccg
    15601 gcaccacaac tttatgctgt gtgtagtcct ggcggtacca gtttgtaccc ttgcttccgg
    15661 ctggtaccgt cctttttaca aaacagagaa gagcatcacc ggacgacggg ctcataaccc
    15721 aatccatccg ggcggctgct accgcaggtg ttcttctctg ttttgtggag aaactaatcg
    15781 gccttgcagg gtcgatatga tgaggagcag caaaatggct agcgaacgca gtactgatgt
    15841 gcaggcattt atcggggagc tggacggcgg cgtatttgaa accaaaatcg gcgcagttct
    15901 cagtgaagtc gcttccggtg tgatgaacac gaaaaccaaa ggtaaggtct cactcaacct
    15961 ggaaatcgaa ccatttgatg agaaccgtgt gaaaatcaaa cacaaactct catatgttcg
    16021 cccgactaac cgcgggaaaa tttccgaaga agacaccacc gaaacgccga tgtatgtcaa
    16081 tcgcggtggt cgcctgacta ttctgcagga agaccaggga caattactga ctcttgccgg
    16141 tgaacctgac ggaaaactcc gcgcagcagg tcattaatat cgttcttaat taactgatta
    16201 tttatctcat cactgaatat ctttatatag ttaggactta ttatgtctca gaacttagac
    16261 gcaaccgcaa ttaatcaaat ccatgccctt atttctgctc agggtgttaa tgaaattatc
    16321 agtaagattg gtgccgatgc tgtggcattg cctgagaatt tccgcattca tgatctggaa
    16381 aaatttaatt taaatcgctt ccgtttccgt ggtgcgcttt ccactgccag catcgatgac
    16441 tttacccgtt attctaaaga tcttgcagat gaaggcaccc gctgctttat cgatgctgat
    16501 aatatgcgtg ccgtcagtgt gcttaacctg ggtactattg atgaaccagg tcgcgcagat
    16561 aacaccgcca ctctcaaact gaaaaagaca gcaccgttct ctgctctgtt gtctgttaat
    16621 ggcgagcgta actcccagaa atcactggca gaatggattg aagactgggc cgactacctt
    16681 gtgggctttg atgctaatgg tgacaccatt caggcaacaa aagcggctgc ggcggtccgt
    16741 aaaatcacga ttgaagcaaa ccagaccgct gattttgaag ataatgactt cagcggcaaa
    16801 cgctccctga tggagtctgt cgaagcgaag accaaagaca ttatgccagt ggcatttgaa
    16861 tttaaatgca ttccgtttga aggtctgaaa gaacgtccgt ttaaattacg cctcagcatt
    16921 atcactggcg atcgtccggt actggttctg cgcattattc agctggaggc ggtgcaggaa
    16981 gaaatggcta acgaatttcg tgatctgctt gttgagaaat tcaaggacag caaagtagaa
    17041 acctttattg gtactttcac cgcctgattt cattactgca aatgcccctg cgggggcatt
    17101 tatggaaacg taatttactc aataatcgcc ggatggtgag ggattctttt taccagaatt
    17161 cagcgcggtg cagcgcatat acgtggagaa caaaatgtca tttattaaaa ctttttccgg
    17221 gaagcatttt tattatgaca ggataaataa agacgacatc gatattaacg atatcgcggt
    17281 ttccctttca aatatctgtc gctttgccgg tcatctttcg cacttctaca gcgtcgccca
    17341 acatgcggtt ctttgcagcc agctggtgcc gcaggaattt gcttttgaag cgttaatgca
    17401 tgatgcaaca gaagcgtatt gccaggacat tcccgcacca ctgaaacgcc ttcttcctga
    17461 ctataaacaa atggaagaaa aaatagactc cgtaatccgt gagaaatacg
//
LOCUS       DAJTYI010000078        17180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYI010000078 DAJTYI010000000
VERSION     DAJTYI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            564..1334
                     /locus_tag="OO564_002545"
     CDS             564..1334
                     /locus_tag="OO564_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6653627.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO564_002546"
     CDS             1325..1798
                     /locus_tag="OO564_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6653628.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO564_002547"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO564_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6653629.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO564_002548"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO564_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6653630.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO564_002549"
     CDS             3233..3727
                     /locus_tag="OO564_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6653631.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO564_002550"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO564_002550"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6653632.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO564_002551"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO564_002551"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6653633.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="OO564_002552"
     CDS             7571..8485
                     /locus_tag="OO564_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6653634.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="OO564_002553"
     CDS             8699..10060
                     /locus_tag="OO564_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653635.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO564_002554"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO564_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6653636.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO564_002555"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO564_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6653637.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO564_002556"
     CDS             12610..13899
                     /locus_tag="OO564_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6653638.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO564_002557"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO564_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6653639.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO564_002558"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO564_002558"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6653640.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO564_002559"
     CDS             16225..16605
                     /locus_tag="OO564_002559"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6653641.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17180
                     /locus_tag="OO564_002560"
     CDS             16673..>17180
                     /locus_tag="OO564_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6653642.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANA"
BASE COUNT         4234 a         4546 c         4294 g         4106 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg
//
LOCUS       DAJTYI010000079        17097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYI010000079 DAJTYI010000000
VERSION     DAJTYI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..820)
                     /gene="epmB"
                     /locus_tag="OO564_002561"
                     /pseudo
     CDS             complement(1..820)
                     /gene="epmB"
                     /locus_tag="OO564_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
     gene            862..1428
                     /gene="efp"
                     /locus_tag="OO564_002562"
     CDS             862..1428
                     /gene="efp"
                     /locus_tag="OO564_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6653643.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1480..1605
                     /gene="ecnA"
                     /locus_tag="OO564_002563"
     CDS             1480..1605
                     /gene="ecnA"
                     /locus_tag="OO564_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6653644.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            1716..1862
                     /gene="ecnB"
                     /locus_tag="OO564_002564"
     CDS             1716..1862
                     /gene="ecnB"
                     /locus_tag="OO564_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6653645.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2038..2267
                     /locus_tag="OO564_002565"
                     /pseudo
     CDS             2038..2267
                     /locus_tag="OO564_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2264..2797)
                     /gene="blc"
                     /locus_tag="OO564_002566"
     CDS             complement(2264..2797)
                     /gene="blc"
                     /locus_tag="OO564_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6653646.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2886..4019)
                     /locus_tag="OO564_002567"
     CDS             complement(2886..4019)
                     /locus_tag="OO564_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6653647.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4082..4441)
                     /gene="frdD"
                     /locus_tag="OO564_002568"
     CDS             complement(4082..4441)
                     /gene="frdD"
                     /locus_tag="OO564_002568"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6653648.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4452..4847)
                     /gene="frdC"
                     /locus_tag="OO564_002569"
     CDS             complement(4452..4847)
                     /gene="frdC"
                     /locus_tag="OO564_002569"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6653649.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(4858..5592)
                     /gene="frdB"
                     /locus_tag="OO564_002570"
     CDS             complement(4858..5592)
                     /gene="frdB"
                     /locus_tag="OO564_002570"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6653650.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5585..7393)
                     /gene="frdA"
                     /locus_tag="OO564_002571"
     CDS             complement(5585..7393)
                     /gene="frdA"
                     /locus_tag="OO564_002571"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6653651.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            7718..8695
                     /gene="epmA"
                     /locus_tag="OO564_002572"
     CDS             7718..8695
                     /gene="epmA"
                     /locus_tag="OO564_002572"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6653652.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            8914..10416
                     /gene="yjeM"
                     /locus_tag="OO564_002573"
     CDS             8914..10416
                     /gene="yjeM"
                     /locus_tag="OO564_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6653653.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(10567..13878)
                     /gene="mscM"
                     /locus_tag="OO564_002574"
     CDS             complement(10567..13878)
                     /gene="mscM"
                     /locus_tag="OO564_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6653654.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(13900..14868)
                     /gene="psd"
                     /locus_tag="OO564_002575"
     CDS             complement(13900..14868)
                     /gene="psd"
                     /locus_tag="OO564_002575"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6653655.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(14965..16017)
                     /gene="rsgA"
                     /locus_tag="OO564_002576"
     CDS             complement(14965..16017)
                     /gene="rsgA"
                     /locus_tag="OO564_002576"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6653656.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16112..16657
                     /gene="orn"
                     /locus_tag="OO564_002577"
     CDS             16112..16657
                     /gene="orn"
                     /locus_tag="OO564_002577"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6653657.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            16868..16943
                     /locus_tag="OO564_002578"
     tRNA            16868..16943
                     /locus_tag="OO564_002578"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16901..16903,aa:Gly,seq:gcc)
BASE COUNT         3943 a         4405 c         4513 g         4236 t
ORIGIN      
        1 aacgcattac tcaggtttgc caacgtttgt gcgttatcgt tcacaccgcg tacagaacgc
       61 tctggttaag cagggtgaca ccgacacggc gcaatttagc catcgcctga cggaacgttt
      121 catctacctc attggcatga ttgatgtgat tcaccagcaa gatttgcagc gtagaacggg
      181 caaagcgttc aaccagcgcc tcggtgatac gtgccgggat cacaatcggc agacggctgt
      241 gaatccgcag acgtttgata tgcgggatgg cttccagttg tgtgagcaac cagtccagct
      301 cgtgatcttt cgccatcagc ggatcgccgc cggagaaaat catctcgtcc agttccggat
      361 gcgcagcaac atactcaagt gcagtctgcc agttacgctt gttgccctga ttttcggcat
      421 aggggaagtg gcgacggaag caatagcggc aatttaccgc gcagccgcct ttgaccagca
      481 aaagcgcccg gttgtggtat ttatgcaaca aaccaggcac tacgctgtgc tgttcttcca
      541 gtgggtcggt ggagaatccg gacgcgacga caaactcatc ttgcgaggta agtacctgac
      601 gcaaaagagg atcgtccgga ttgcctttct ccatgcgatc gataaatgag cggggcacac
      661 gcagggcaaa cagctttttg gcgctgcgtc cggccaacag tttttcgtcc gcgtctatat
      721 tcaaaagacg cagaagttca tcaggatcgg tcacaacatc ggcaagttgc gttaaccaat
      781 cttctctgga tggggtattt agggttacaa tatgcgccat tttgtggctt agctaccaat
      841 taacaaattt cagagggcct tatggcaacg tactatagca acgattttcg tgctggtctt
      901 aaaatcatgt tagacggcga accttacgcg gttgaagcga gtgaattcgt aaaaccgggt
      961 aaaggccagg catttgctcg cgttaaactg cgtcgtctgc tgaccggtac tcgcgtagaa
     1021 aaaaccttca aatctactga ttccgctgaa ggcgctgatg ttgtcgatat gaacctgact
     1081 tacctgtaca acgacggtga gttctggcac ttcatgaaca acgaaacttt cgagcagctg
     1141 tctgctgatg caaaagcaat tggcgacaac gctaaatggc tgctggatca ggcagagtgt
     1201 atcgtaactc tgtggaatgg tcagccgatc tccgttactc cgccgaactt cgttgaactg
     1261 gaaatcgttg ataccgatcc gggcctgaaa ggtgataccg caggtactgg cggcaaaccg
     1321 gctaccctgt ctactggcgc tgtggttaaa gttccgctgt tcgtacaaat cggcgaagtc
     1381 atcaaagtgg atacccgctc tggtgaatac gtctctcgcg tgaagtaatg cggttgtggt
     1441 gcggcctgca ggctgcacca tcacttattc aggtcagaga tgatgaaacg ccttatcgtt
     1501 cttgttttgc ttgccagcac gctgctcacg ggctgtaaca ccgcttgcgg tttcggcgaa
     1561 gacatcaaac atctcggcaa ctccatctct cgcgctgcca gctaattttt cttctcttcc
     1621 gaaaaatcat cagatttcca tcatttttgg cgatgttgtc tattattaat ttgctatagg
     1681 caaacataaa taacattacc taaaaggaag acgttatggt gaagaagaca attgcagcga
     1741 tcttttctgt tctggtgctt tcaacagtat taactgcctg caacaccacg cgtggcgttg
     1801 gtgaagacat ttctgatggc ggtaacgcga tttctggcgc agcaacgaaa gcgcagcaat
     1861 aagcaataac ggtacgacag cagtgtcgtg ccgtttgttt tttctgcgat cgtcataaag
     1921 gtaatagttg aaattcccct gccacctggc aaaatatccg ttcaaccatc agctttgcag
     1981 gacgacctgc aaacgcctct tttcaccggg gacggcccca aatttccgga gcctgatatg
     2041 tcctggatta tcttagttat tgctggtctg ctggaagtgg tatgggccgt tggcctgaaa
     2101 tatacccacg gctttagccg tttgatgccg tgtggacggg tattggcgca gtcggcgcgg
     2161 ccatcaccgg cattgtgctg ctcggtgagt ccgctaaccc gatgcgtctg gcgagtctgg
     2221 cgttaatcgt attggggatt attggtctga aactcagcac tcactaacta ccgggctgct
     2281 gtacccaaat aaacttactg acatcaaacc cttcccgggt cgcgactgcc agcatctcct
     2341 gtttcacttc gtcagaaatg gttggcgtgc gggagagtat ccacaggtag tcgcggtccg
     2401 ggccgcaaac cagcgcatgg cggtattccc gatcgagtgc aataacgtta taaccgccat
     2461 agaaaggacc aaagaatgac actttcagcg cagcgcgagt tggtgcgccg gtaaagtacg
     2521 ctttcccttc actctgctgc cacattcctc tgtcagggtt atagccttta ttaatgacat
     2581 tcaggccgcc gtcatcacgc aggctgtatg ttgcggtgac tttttccagt ccacgttcaa
     2641 aacggtgatc aaaacgggca atctcatacc aggtaccgag ataacgtttg gcgtcgaaat
     2701 tattcactac ggtcacgcca cgcggcggcg taggagaact acaggcaacg accagaaatg
     2761 cagctgtcgc tgcggcaacg agagggagca ggcgcataaa tgtttcctta ctggtttttt
     2821 tctaagtgta gatgacagca aggaaaagcg cagaaaaggt ccgaaaattc ggacccgatg
     2881 aaattttact gtagagcgtt gagaatctga caggcggcgg cgactctctc tggattggga
     2941 tagtttttgt tagccagcat cacgataccc agctcttttt ctggaataaa cgcgacatag
     3001 ctaccaaatc cgccggtcgc ccccgtttta tgtacccatg atgcgcgtac tgcaggagtt
     3061 gggggcttaa tcgcttttac cgggcgtgct gccagtgcaa ttttattgtc actgccgtta
     3121 atgatgctgt caggattgac cggccagtcc agcatttccc agcccaggcc ctgatacata
     3181 tcgccggttt gccagtagcg agattgtgcc agttgtatcc cttgttgaag cgttttctca
     3241 ttgatatcaa gggggggtaa attgctttgc acccagcggg ccatatcttc aatggtcgac
     3301 ttcacaccat aagcttcagc atctaacgcc cctggcgaaa catgcactgc cttaccttcg
     3361 cgatatcccc aggcgtaatt cttttcttct gggggaggta cattaatcca cgtatggttg
     3421 agtttgagtg gctggaagac acgagtttgc atcgcctgct caaaactcaa accagacggc
     3481 ttcacagcca gtgcgccgaa caaaccgata ctggagttgg catacagacg ttgtgttcct
     3541 ggggcccatg caggctgcca gttttgatag aagcgcagca agtcgcttga ggatttcact
     3601 tcatccggca cctgcaatgg caggccgcca gcggtgtagg tcgcaagatg taatagtgtg
     3661 atcccattcc actgtttagc ggtaagttca ggccagtatt ttgttgcggg atcgcttaac
     3721 ttgatttccc ctcgagcaat agcgtcgcca ccaagcacgc ccgtaaatgt tttgctgacc
     3781 gaacctaact caaacaacgt ttgctgtgtg acgggctgct ttttggcgat gtccgcatag
     3841 ccccaagtaa agtaataagg tttaccctga taaattaccg ccaccgccat accggggatc
     3901 ttttgttgct ctataagcgg ggtaattgtg cgatgcacaa tatcgttgat ttgttgaggg
     3961 gcagcaaatg tggagcaaga ggcggtaatt aataaggcgc agagcgtcgt tttgaacata
     4021 aggtctggtt tccatacaaa acggcccgcc ataggcgggc cggatttaca ttggcgatgc
     4081 gttagattgt aacgacacca atcagcgtga caactgtcag gatagcagcc agaccgtaga
     4141 aaacccattt gcccgcaggc acgtggattt tcagatcgtg catcgcgtgg tgcatacggt
     4201 gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga ccaatgaagc
     4261 tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac agccccagtg
     4321 gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc cacataccac
     4381 cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt ggattaatca
     4441 tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc aaccacagtt
     4501 accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt tacaatgata
     4561 ttggccgctt tcggtgccag ttcaaaccag gttttggtgt gcagcagagc tgccgtcaga
     4621 gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa tcccgcccag
     4681 gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct gaaccacaca
     4741 gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa ttttttccac
     4801 caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt gcactcctta
     4861 gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc cctgctgaat
     4921 ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca cgaaagtaca
     4981 gctccataca ccgttctggc tgttcaactg cgccatacgc tccttcttac cgtggtcgcg
     5041 gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga actctgggtt
     5101 caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac cggagaactg
     5161 gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg cggtgcggga
     5221 gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg tcatatcgac
     5281 caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac cgtcggtgta
     5341 atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca tgccgcagga
     5401 accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca ggttgtcttt
     5461 gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt catagaatgc
     5521 gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt tcaggttttt
     5581 catctcagcc attcgccttc tccttattat tggctgcttc cgccttatcg gctgcatccg
     5641 cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg tcgctgtact
     5701 ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgaga aagttgacgt
     5761 cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac tctttacgtg
     5821 ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggctcagt tcaatggtgt
     5881 agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc ttgaagcgtt
     5941 cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta cggtagatac
     6001 cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag ttttcgccgc
     6061 catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc gcttcaattg
     6121 ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca ccggccagac
     6181 ggccgaagac caccagttcc gccagggagt tggagcccag acggtttgca ccgtgcagac
     6241 caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca cagttctgat
     6301 cggtttcgat accgcccatg gtgtagtgtg cagtcggacg taccggaatc ggttctttaa
     6361 ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga cgttcatgca
     6421 gttttttctc gccgaggtga cgcaggtcga gataaaccac atcaccacgc ggcgtggaga
     6481 tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg cgtggaccca
     6541 gttccatata tttgtttttc ggctcgccca gcggagtttc cgggcccatg ccgtaatctt
     6601 gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccacgg cagccttcgg
     6661 tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat tccatgtcac
     6721 gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg atgccgccgt
     6781 tggtgttgta acgataaacg cgacccgcac cgccggtagc cataacgacc gcgttagcac
     6841 ggatctgcac caacgtgcct tccatcatgt tcattgctac caggccgcga acatgaccat
     6901 catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc gggaattgca
     6961 gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg gcgaaccagg
     7021 tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc gggcgacggc
     7081 tccatgggca tccccacagt tccagttggg tcatttcggt tgggcagtgg tggacgaaat
     7141 aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg tgaaagtgat
     7201 attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca gcaacggtat
     7261 ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcatttgga tttgcctgcg
     7321 cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca agatcggctt
     7381 gaaaggtttg cacgacattc ctccagattg tttttatccc acagccccgt acttcagggt
     7441 aagtgcctga aagttacggt ctgcgaacgc tattccactg ctcctttata ggtacaacag
     7501 tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg cgcaagaggg
     7561 ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt gcttttttta
     7621 gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg cgttgcgagt
     7681 agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg catcctggca
     7741 gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg agatccgtcg
     7801 tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc aggcgacggt
     7861 aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc attcgcaggg
     7921 gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgcctgc tggttgctgg
     7981 ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg ggcgttatca
     8041 caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt accggttgat
     8101 gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag aaagcctttc
     8161 ttatcaacaa gctttcttgc gttatctgga aattgacccg ctctctgccg acaaaacgca
     8221 actgcgggaa gtcgcagcga aactggattt gagcaatgtt gctgataccg aagaagaccg
     8281 cgacacgttg ctacaattgc tgtttacctt tggcgtagag ccaaatattg gtaaagaaaa
     8341 accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa tcagtaccga
     8401 agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc tggcgaatgg
     8461 tttccatgaa ttaacggatg cccgtgagca gcaacaacgc tttgaacaag ataaccgtaa
     8521 gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg aagccttgaa
     8581 agtcggtatg cctgactgct ccggcgtggc attaggtgtt gatcgtctgg tgatgttggc
     8641 gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg cataattctg
     8701 aaaaattact gaattaacag cgaagaatgg cgtgataagc gccattctgt agcatatgtt
     8761 ttcatttttc cgttaaaggc cacaatttaa gactggatat ttgatatcat caaggtatca
     8821 attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc agtgggcacg
     8881 tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa aaagatgagt
     8941 ctgataggac tcatattgat gatctttact tccgtatttg gatttgccaa tagcccatcg
     9001 gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc attgttattc
     9061 tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa agaagaaggt
     9121 ggtatctatt cctggatgaa taatagtgtc ggaccacgtt ttgccttcat tggtacgttt
     9181 atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt ttgggtaccg
     9241 ttctcaacat tcctctatgg tagtgacatg acccagcact ggcgtattgc cggactggag
     9301 cctacgcagg tggttggtct gctggcagta gcatggatga ttctggtcac cgtcgttgct
     9361 tcaaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc agtaatgtgt
     9421 ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg tgggcatttc
     9481 gcgcaggata ttaatttcct tgcatcaccg aacccgggtt atcagtccgg tctggcaatg
     9541 ctatcgtttg tggtatttgc tatttttgcc tatggcggaa ttgaagcggt tggtggtctg
     9601 gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt tgccgctatt
     9661 gttatttcaa tcggttattc gctggcaata tttttatggg gcgtcagcac aaactggcag
     9721 caggtattaa gtaatggttc cgttaacctt ggcaatatta cctatgtgct gatgaagagc
     9781 ctcggggtga cgctgggtaa cgcactgcat ttgtcacctg aagcgtcatt gtcgctgggc
     9841 gtatggtttg cgcgtattac cggactttcg atgttcctcg cttataccgg tgcgttcttt
     9901 acgctttgct attcaccgct gaaagccatc atccagggga cgccgaaagc gttgtggccg
     9961 gaaccgatga cgcgcctgaa tgcgatgggg atgccgtcta tcgccatgtg gatgcagtgc
    10021 gggttggtta ctgtcttcat tctgctggtt tcgtttggtg gcggtaccgc atcggcgttc
    10081 tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttacct gttcctcgcg
    10141 ctggctttcc cgttttttaa agcacgtcag gatctcgaca gaccatttgt gattttcaaa
    10201 acgcgtatgt cggcaatgat tgcgacggtg gttgtcgttc tggtggtgac atttgcgaac
    10261 gtcttcacca ttattcagcc tgtggttgaa gccggagact gggacagcac attgtggatg
    10321 attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa ctattgcagt
    10381 cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct gcaactcatc
    10441 gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct ccagggccgg
    10501 tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat ttggcagctt
    10561 catgcattac aaacttcccg cctgacgacc tttgcccgca gacgtcagcg ttctccccgt
    10621 ttgtttaccg ttgaggcttt ccagacgcat ctggaagggc gggaatggca tatcgatacc
    10681 gtgggcgtgg aagcctgcca gaatcagctg gtggatctca tggcgtagcg gcatacggtg
    10741 acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccctgtt gcagatccac
    10801 caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg ctgcggtgag
    10861 caggatttcc gtcacttctt cactgttggc atcggcaggg gccggaatcg tcaacactac
    10921 gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa acgccttgtt
    10981 cggcacgatt atctctttac ggtcccagtc gctgatggtg gtggcgcggg tgttaatttt
    11041 tgtcacgcta ccagtaagat cgcggatcgt taccgtatcg ccaatgcgaa tcggtttttc
    11101 gaacaggatg atcaggccag agataaagtt ggcgaaaatt tcctgcaaac caaaaccgag
    11161 accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac caatcattga
    11221 gaagccgacc agcccgccaa tcagcatcag cagatatttt gtgatggtgg tgatggcgta
    11281 acccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca gcgcgggcag
    11341 gttgcgcacc agctgcgtgg tgatgataaa taccagaatg gcaatcagca ccgcaccgag
    11401 ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc acagcgaaat
    11461 attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga ccgaaagcag
    11521 ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga tggcatcgag
    11581 atcgacttcg ctttcatcga cttcaattgc tccttccggg ctactgtgat gatgcgcttc
    11641 ctcttcgcca cgcgcacgtt gcgctaacat ctctgcccgg cgatgcttcg cccggtcaaa
    11701 cgccagcctg cgacgctgga tcagcatcca gcggcggata acgtgataaa ccaccagtag
    11761 caggaaccag atggcaatcg aggtttcaag cctcgctaac agtgcctgcg ccgttgccag
    11821 ataacccacc gccgacgcca gaatggcaac cagtggtgcg ccaatcatca tgttccacag
    11881 catatggttg gtaatgttgt cgccgctgcc ttctttgttg agatacaacg gaataccggc
    11941 ttttttcagg cttagggtga ccaccgccag cgcaccgcaa atgaggataa agcaaagccg
    12001 tcccagcgaa ccggaaaatt cgcggtcgtc gaggttatcg aacatcatca gcgccataat
    12061 cagcccgatg ctcatcaggt agtagcgcat cccacgggaa acacgttcgc gcggccagcc
    12121 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggtg gcgcaaatca tcactaccca
    12181 cagcagcggc acggtggccg ttacaccatc gccaatcgcg accgccagcg gataaggcca
    12241 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggtaacggtg acgcgacgag
    12301 aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga tcctgagtca ctttgccgac
    12361 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
    12421 gaccagaatc aacgcgccaa acagcggcaa aattgtctct ttgctggtca gcatcatcac
    12481 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
    12541 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
    12601 gtgcgttgct tcgttcacct ctttcagcgc atcctccagt tgcccgttgg agactttcag
    12661 cttggtcagt tccaatagta gcgtgtcgcc accctgcaac aatgagttca gcaactcacg
    12721 ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg gcggtcagcg gctgaccgtc
    12781 ggcctgatgg atttgccgta gcagcggctg tttattaagt aaatcctcat aacgtaaccg
    12841 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggttttggca tttctggtag
    12901 ccgtgccacc tgcgcccgca gcgcttcgcc gagcaggttg gacgatccca gccattgcga
    12961 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
    13021 ctgttgcgag gcaacgagct ccatccgctg cgcctgttga ttcagcgctg ccgatagttc
    13081 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgccag
    13141 taattcggta ctttccagcg cccgctccgc ctcaagctga cgttggctat tcaactgatt
    13201 acgtaagccc tgcaaatacg catccagttg ctggctctct ttttccgcca gctctgagcg
    13261 taagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
    13321 gagcgcctta agacgtgcag agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
    13381 agtattgccg gtaagcgttc ccaggcggcg ctcgatctca tttaactgac ggcgggcgtc
    13441 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
    13501 ttgctgggcc tgacggcttt tatccagcaa ctggctgctg acctggagaa tttcctgatt
    13561 cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
    13621 ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg ataacttgct gatattgttt
    13681 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagcgcctc
    13741 tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc tccagttcct gagtgatttg
    13801 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
    13861 agtgataatc aggcgcacgt cagcgtttcc tttgatggat tagacctggt cttttttatc
    13921 gtcaaccaat ggcctggcgt cgtgttctgc ttcgatctct tcagcaggaa gtggggcggg
    13981 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
    14041 gacagacagg ctttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
    14101 aacggtggaa ccgagtttaa agcgacccat ttcctggcct ttcagcagtg ccacagaacc
    14161 gtcgttttcc ccggcaggcc aggtccagcg cttgatgata ccttcgcgcg gcggcgtaat
    14221 ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc gctccgacca gaatctgcgc
    14281 cattgggcca aattcggtat cgaaaaggca aatcacgcgt tcgttacggg caaacagatt
    14341 cggcacgttc tgagcggtga gatggttaac ggaaaagaga tcgcccggca cgtagatcat
    14401 ctcacgcaga ataccgttgc acggcatgtg tacgcgatgg tagtcacgcg gggagaggta
    14461 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc agatagttgc ctgccagcag
    14521 ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
    14581 caactggctg ataacgccat cggcaggcat gaccagtaca ttcggatcgg tatcgattgg
    14641 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
    14701 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
    14761 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
    14821 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaagg tagcctccag
    14881 gccattgttt tgtcgttcct gatccggcca acatgccgga tcctgaaaaa aaggggacga
    14941 ttctaacgac ggttagctta attgtcagcc atccgtatca gaaaagtttt tacgcgtttt
    15001 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
    15061 cccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
    15121 atatttacac agacctaaat agtcatggaa ttcgacaaag ccctgagtga tttgttccgg
    15181 ctccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
    15241 cgggaagtga tacagccgag cggcggtcgt ggtgtgctga ccgagccccg agttgtcaga
    15301 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
    15361 accgacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
    15421 tttcagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tgttgcggta
    15481 gatatccatc tgctcgttga cgaacgccat gccttcgtcg tccagcaggt ctatcttgtt
    15541 gagcacgata atcggctcaa tctgcaaggt ttcgcaggcc accaggtaac ggtcgataat
    15601 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
    15661 tatgggtttc acgccgtcgt agaaatccgg acgcgttaac accgaggtgc gctcatgcac
    15721 cgcttccacg atccctttga tatttacgcc ttccgccgcc ggtttacccg gacgccagac
    15781 tacgcggtcg ccggttacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
    15841 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
    15901 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
    15961 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
    16021 tactgactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
    16081 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
    16141 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
    16201 gctggtgacc gatgccaatc tgaatattct ggcagaaggg ccgaccattg cagtacatca
    16261 gtccgatgaa cagctggcgc tgatggatga ctggaacgtg cgcacccata ccgccagcgg
    16321 gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg gaagctgaac tggcaacgct
    16381 cgaattctta aaacagtggg tgcctgcggg aaaatcgccg atttgcggta acagcatcgg
    16441 acaggaccgt cgtttcctgt ttaaatacat gccggagctg gaagtctact tccactaccg
    16501 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattctgga
    16561 tggttttacc aagcagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
    16621 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttcctggt cacgtaagcg
    16681 ccggaatcca cttgcggcgg cgtgattttg acgctaaatc gaacgtcttg ctgataattt
    16741 cagcacttga gataaaaacg caaaaaaaac ttttttgggg ggttgcagag ggaaagattt
    16801 ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
    16861 gtagtatgcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag
    16921 ttcgagtctc gtttcccgct ccaaaatttg aaaagtgctg caaagcacag accacccaat
    16981 cgcagtattt cgaattgcga caaggcggca actgaacgaa tccttgggaa cttacaaaag
    17041 tatgtgacta aggtgagtga aggcagccaa ggcagtagcg gttcgaaaga cgaagat
//
LOCUS       DAJTYI010000080        16911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYI010000080 DAJTYI010000000
VERSION     DAJTYI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            338..1157
                     /gene="ybhA"
                     /locus_tag="OO564_002579"
                     /pseudo
     CDS             338..1157
                     /gene="ybhA"
                     /locus_tag="OO564_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1158..2216)
                     /gene="modC"
                     /locus_tag="OO564_002580"
     CDS             complement(1158..2216)
                     /gene="modC"
                     /locus_tag="OO564_002580"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6653658.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2219..2908)
                     /gene="modB"
                     /locus_tag="OO564_002581"
     CDS             complement(2219..2908)
                     /gene="modB"
                     /locus_tag="OO564_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6653659.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2908..3681)
                     /gene="modA"
                     /locus_tag="OO564_002582"
     CDS             complement(2908..3681)
                     /gene="modA"
                     /locus_tag="OO564_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6653660.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3848..3997)
                     /gene="acrZ"
                     /locus_tag="OO564_002583"
     CDS             complement(3848..3997)
                     /gene="acrZ"
                     /locus_tag="OO564_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6653661.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4126..4914
                     /gene="modE"
                     /locus_tag="OO564_002584"
     CDS             4126..4914
                     /gene="modE"
                     /locus_tag="OO564_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6653662.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4982..6454
                     /gene="modF"
                     /locus_tag="OO564_002585"
     CDS             4982..6454
                     /gene="modF"
                     /locus_tag="OO564_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6653663.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6715..7731
                     /gene="galE"
                     /locus_tag="OO564_002586"
     CDS             6715..7731
                     /gene="galE"
                     /locus_tag="OO564_002586"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6653664.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7741..8787
                     /gene="galT"
                     /locus_tag="OO564_002587"
     CDS             7741..8787
                     /gene="galT"
                     /locus_tag="OO564_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6653665.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8791..9939
                     /gene="galK"
                     /locus_tag="OO564_002588"
     CDS             8791..9939
                     /gene="galK"
                     /locus_tag="OO564_002588"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6653666.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9933..10973
                     /gene="galM"
                     /locus_tag="OO564_002589"
     CDS             9933..10973
                     /gene="galM"
                     /locus_tag="OO564_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6653667.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11176..11928
                     /gene="gpmA"
                     /locus_tag="OO564_002590"
     CDS             11176..11928
                     /gene="gpmA"
                     /locus_tag="OO564_002590"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6653668.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12095..13147)
                     /gene="aroG"
                     /locus_tag="OO564_002591"
     CDS             complement(12095..13147)
                     /gene="aroG"
                     /locus_tag="OO564_002591"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6653669.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13463..13843
                     /locus_tag="OO564_002592"
     CDS             13463..13843
                     /locus_tag="OO564_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6653670.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13957..14898
                     /gene="zitB"
                     /locus_tag="OO564_002593"
     CDS             13957..14898
                     /gene="zitB"
                     /locus_tag="OO564_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6653671.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14895..15614)
                     /gene="pnuC"
                     /locus_tag="OO564_002594"
     CDS             complement(14895..15614)
                     /gene="pnuC"
                     /locus_tag="OO564_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6653672.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15652..16695)
                     /gene="nadA"
                     /locus_tag="OO564_002595"
     CDS             complement(15652..16695)
                     /gene="nadA"
                     /locus_tag="OO564_002595"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6653673.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         4279 a         4436 c         4198 g         3998 t
ORIGIN      
        1 gaaaaaggca gtgatttcac tcaggcgcac cttggacatc gttcatccag catgacggat
       61 aaataccgtg acggacgagg gagggaatgg aaagacattt agaattaatt tcgaatgatt
      121 tcgaaatacc acttgtaatc aattgattat aaaggattaa ttcctaaaga gcatttactt
      181 catgaatgct cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc
      241 ggtgaatgct gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc
      301 gcagacgaaa attcttaacc ttaatctgga atacgccatg accacacgcg tgattgctct
      361 cgacttagac ggcaccttat tgaccccgaa aaagaccttg cttccatcat cgatagaagc
      421 cctggcccgc gctcgcgaag caggctatca attaatcatc gtcacaggtc gccatcacgt
      481 cgctattcat ccttttttat caggcgctgg cgctggatac acctgctatt tgctgtaatg
      541 gcacctattt gtatgattat catgcaaaaa ccgtgctgga agcggaccca atgcccgtta
      601 ataaagccct gcaactcatt gagatgctga atgaacacca cattcacggt ctgatgtatg
      661 tcgatgatgc aatggtctat gagcacccga ccgggcatgt cattcgcacg tctaactggg
      721 cgcaaaccct gccgccggaa cagcgtccga ctttcacaca agtcgcttct ctggctgaaa
      781 cgacgcaaca agttaacgcc gtatggaagt tcaccctgac gcacgatgac ctgccgcaat
      841 tgcagcattt tggtaagcat gtcgaacatg aactgggact ggagtgtgaa tggtcctggc
      901 acgatcaggt tgatattgca cacggcggca acagcaaagg taaacgtttg acgaaatggg
      961 ttgaggcgca aggttggtcg atggaaaacg tcgtggcatt cggcgataac tttaatgata
     1021 tcagtatgct ggaagccgct ggtacaggcg tggcgatggg caacgccgat gacgcggtaa
     1081 aagcgcgcgc caacattgtg attggtgata acaccaccga cagcattgcc cagttcattt
     1141 atagccacct gatttaatca ggcggttatc gacacacttt taatttgcgc gtacagccac
     1201 aggccaggtt tgatcgccag ttcatccctg gcccacgggc tgatacgcgc ccacagcgtt
     1261 ttaccgccga cttccagctc cacttccacc tggccgttgt cgtcataact attaacaact
     1321 tttgcccgca atacgttacg aatgctggtt tgctgcggcg gttgtaaaac cagagaaaca
     1381 tcggaagcct gaatgcggat acgtagcgca gcttgcagcg gttcgcccag cttattgacc
     1441 cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt aatgcggatg atgctcaagc
     1501 accgtcactt tcagaatgct actttgttgc tctttcggca gccacggatt catcacgcta
     1561 ctgccccaca cttcctccag cgcgccaaag gctttcacct gaccgttttc cagtaccatc
     1621 actctgtctg ccagatggag gatctcatcc agcgaatggc tgacatacaa catcggaatg
     1681 ttgatttccc gtgtcagccg ttgcagataa ggcaacagtt cgcgtttacg cggaatatcc
     1741 agtgacgcca gcggttcatc cagcaacagc aattccggtg ctgtcagcaa agcccgacca
     1801 atcgccacgc gctgtttttc gcctccggac aggctgcctg gtaaacggtc aagcaacggt
     1861 tcaatgccta aaagcgccac cagcttatcg aactgatcga ccatactttt cgacatgccg
     1921 tagcgcagat tgccacgcac tttgtaatgc gggaacaacc gcgcatcctg aaaaacatag
     1981 ccaacacgac gcttttccgg cgtcaggcag ataccttttt cggcatcatt tagtacccgc
     2041 ccattgagga caatccgccc tttttgcggg cgcgtcagtc cactgatggc gttaatcagc
     2101 gaagtttttc cggcaccgga gacgccaaag atagcagtga tgccattggc gggcagcgtt
     2161 tcattaatgg tcaggcaatg gttgcccaac gtctgggaaa aattcagctc cagcatgatt
     2221 agcgccccgc ccgttcacgg ctgattctgg ccagccattc tgaaatcaac agggagatca
     2281 tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc actttccccg ccgggggtct
     2341 ggatcagggt atacatggca gaaggaatgg ttcgcgtttc gctaggaatg ttcgacacaa
     2401 aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa agccagtacc gtaccaacaa
     2461 taattcccgg taaggtcagc ggtaacgtga tagtaaagaa aacgcgccag cgcccggcac
     2521 ctagtgttct tgcggcctgt tccagtttga catcaacccc ttccagcgcc agacgaattg
     2581 cccgcaccat cagcggaaac gacatgacgg cggcagcgag taccgcgccg cgccagctaa
     2641 aggcgaaggt aataccaaac cagtcataca gacgttcacc gataaatccg cgccgtccca
     2701 tcgaaactaa taataagtaa ccgacgacca cgggcggtaa caccagcggt agatgcagta
     2761 cgctgtcgag cagagctttg cccggaaacg tgcaacgcac cagtaaccag gcaaaaaaga
     2821 tcccaaacgg caggctaaac agcacagcca gggaagaaac tttcaggctt aataaaactg
     2881 cctgccattc tggatcggtc agtatcatta cttggttgta aatccgtaac gtttaaagat
     2941 ttccgctgcc tgcggtccct tcagataatc ataaaatgct ttcactgtcg cattgttatg
     3001 cccttccaca acagcaaccg gatattccac ttttttatgt gaatcttccg ggaaggtggc
     3061 aaccactttt acccctttgc tggcaactgc gtcagaaccg tagacaatgc ccagaggcgc
     3121 ttcgttacgt tcgaccagcg ccagcgcccc acgaacatct tccgctgggg ccagtttcgg
     3181 agagagcgta tcccatgcgc ccagtttttg cagtgcttct tttgcataaa tgccagcggg
     3241 aacatgttcc ggatcgccaa ccgccaggcg accgccattc agcagcgaag tccagttagt
     3301 tttgctgtcg atggtgaaat ctttctgctc gctggctttc ggtgctacaa cgaccaggct
     3361 attgccgagc agtgtctgac gcgtagcagt atcgatcgct tttttatcaa ccgcataatc
     3421 catccatttc tgatcggcag aaataaacag atccgcaggg gcacccgctt caatctgacg
     3481 ggcgagagta gatgacgaag cgaaagaaga aaccacatcc actcctttct cttttttata
     3541 ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg gcggcgaaca ccgtgatttt
     3601 cccttcatct gccagtgcat taccagcaac tgcgaaagag agtgccgccc cggcaaacaa
     3661 gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa cgttatgtag gcgacaatat
     3721 aacgactaac tcggaatttt cccagctgtt attgctaacc tttgtgaggt agataagaaa
     3781 aaatatcggc aggaaaagca ggaagttgag aaaaagaaaa tgcccgacta agcgggcatt
     3841 cagggaatca atgattttgt ccgggctggt cttttttacc aacaccagaa aagatgttga
     3901 atacttcacc aagaccgtaa atcagaccca ggatgatggc catcacgaca ggtaccatga
     3961 ttacggcgaa taccagactt tttaataact ctaacatggt caactccaga tatagtcatg
     4021 agactattct aaccgctaag cacagaaaag cactcccctt ttgtgcggtc agctttgcgt
     4081 ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca ttgttatgca ggccgaaatc
     4141 cttctcaccc ttaagctcca acaaaaatta ttcgccgacc cgcgccgcat ttcgctacta
     4201 aaacacattg cgctttccgg ttccattagc cagggagcga aagatgccgg tatcagctat
     4261 aaaagcgcct gggatgccat taacgagatg aatcagttaa gtgagcatat tctggtcgag
     4321 cgcgcaacag gcggtaaagg tggcggaggc gcagtactga cccgctatgg tcagcgactg
     4381 attcagctct atgacttact ggcgcaaatc cagcaaaaag cctttgatgt gttaagtgat
     4441 gatgacgccc tgccgctgaa cagcctgctg gccgcgatct cacgtttttc actgcaaacc
     4501 agcgcccgta accagtggtt cggtaccatc accgcccgcg atcatgatga cgttcaacag
     4561 catgttgatg tcttactggc tgacggaaaa acacgcctga aagtcgcaat taccgcacaa
     4621 agcggcgcac gtctggggct ggatgaaggc aaagaagtgt tgatattgct aaaagcgccg
     4681 tgggtaggca ttactcagga cgaagcggtc gcacaaaacg ccgacaacca attaccgggt
     4741 attattagtc atattgagcg cggcgcagag cagtgcgaag tattaatggc gctacccgac
     4801 gggcaaacac tgtgcgccac agtgccggta aatgaagcga cttctcttca gcaagggcag
     4861 aatgtcacgg cctactttaa tgccgacagc gtgattatcg ccacgctgtg ctaagcgtgt
     4921 tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg ctgcacaaaa tgggatataa
     4981 aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt agcgacacaa aaacgctgca
     5041 attgcctcag ctaacgttaa acgcgggtga tagttgggcg tttgtcggtt cgaatggaag
     5101 tggaaagtcg gcgctggccc gcgcgctggc gggggaactt ccgcttttga aaggtgaacg
     5161 gcaaagccag ttttcccaca tcactcgtct ctccttcgag caattgcaaa agctcgtcag
     5221 cgacgaatgg caacggaata acaccgatat gctcggccct agcgaagatg acaccggacg
     5281 cactacggct gagattattc aggatgaagt aaaggatgca ccgcgttgca tgcaactggc
     5341 gcagcagttc ggtattaccg ccctcctcga ccgacgcttt aaataccttt ccactggcga
     5401 gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag cctgacttgt tgattcttga
     5461 tgagccgttc gatggcctgg atgttgcctc acgtcagcag ctggctgagc gactcgcctc
     5521 gttacatcag tccggtatta ctttggtact ggtgctcaat cgcttcgata agatcccgga
     5581 atttgtccag tttgctggcg tgctggcgga ttgtacgtta gcggaaactg gcgctaaaga
     5641 ggaactgctc cagcaagcac tcgtcgcgca acttgcgcat agtgaacagc ttgaaggtgt
     5701 gcaactgccg gagccggatg aaccttcagc acgtcacgcc ttacccgcca acgaatcgcg
     5761 cattgtgctg aacaatggcg tggtttctta taatgatcgc cccattctta ataaccttag
     5821 ctggcaggtg aatccaggcg aacactggca aattgtcggg ccaaatggcg cgggaaaatc
     5881 gacgttatta agcctgatta ctggcgatca tccgcaaggt tacagcaacg atttgacgct
     5941 tttcggccga cgtcgcggca gcagcgaaac catctgggac atcaaaaagc atatcggtta
     6001 cgtcagcagt agtttgcatc tggattaccg ggtcagcact accgtgcgta atgtgattct
     6061 ttctggctat tttgattcga ttggcattta tcaggccgtt tcggatcgcc agcaaaaact
     6121 ggtgcagcag tggctggata ttctcggcat tgataaacac acggctgacg ctccgttcca
     6181 tagtctttcc tggggacagc agcgtctggc gctgatcgtc cgcgcactgg tgaaacatcc
     6241 gacgttgctt attctcgatg aaccactaca ggggcttgat ccgctcaatc gccaacttat
     6301 ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg caattgttgt ttgtttcgca
     6361 ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt gagttcgtgc cggacggtgg
     6421 actctatcgc tatgtgctga caaaaatata ttgagtcggt agtgctgacc ttgccggagg
     6481 cggcctaagc gccctctccg gccaacggtt cgacgcatgc aggcatgaaa ccgcttcttt
     6541 tttcagataa aaagcgcaat cattcataaa ccctctgttt tataatcact taatcgcgca
     6601 taaaaaacgg ctaaattctt gtgtaaacga ttccactaat ttattccatg tcacactttt
     6661 cgcatctttg ttatgctatg gttatttcat accataagcc taatggagcg aattatgaga
     6721 gttctggtta ccggtggtag cggttacatt ggaagtcata cctgcgtgca attactgcaa
     6781 aacggtcatg atgtcatcat tcttgataac ctctgtaaca gtaagcgcag cgtactgcct
     6841 gttatcgagc gtttaggcgg caaacatcca acgtttgttg aaggcgatat tcgtaacgaa
     6901 gcgttgatga ccgagatcct gcacgatcac gctatcgaca ccgtgatcca cttcgccggg
     6961 ctgaaagccg tcgccgaatc ggtacaaaaa ccgctggaat attacgacaa caatgtcaac
     7021 ggcactctgc gcctgattag cgccatgcgc gccgctaacg tcaaaaactt tatttttagc
     7081 tcctccgcca ccgtttatgg cgatcagccc caaattccat acgttgaaag cttcccgacc
     7141 ggcacaccgc aaagccctta tggcaaaagc aaattgatgg tggaacagat cctcaccgac
     7201 ctgcaaaaag cccagccgga ctggagcatt gccctgctgc gctacttcaa cccggttggc
     7261 gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca ttccgaataa cctgatgcca
     7321 tacatcgccc aagttgctgt aggccgtcgc gactcgctgg cgatttttgg taacgattat
     7381 ccgaccgaag atggtactgg cgtacgcgat tacatccacg taatggatct ggcggacggt
     7441 cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg tacacatcta caacctcggt
     7501 gctggcgtag gcagcagcgt gctggacgtg gttaatgcct tcagcaaagc ctgcggcaaa
     7561 ccggttaact atcattttgc accgcgtcgc gaaggcgacc ttccggccta ctgggcggac
     7621 gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc gcacactcga tgaaatggcg
     7681 caggacacct ggcactggca gtcacgccat ccacagggat atcccgatta aggaacgacc
     7741 atgacgcaat ttaatcccgt tgatcatcca catcgccgct acaacccgct caccgggcaa
     7801 tggattctgg tttcaccgca ccgcgctaag cgcccctggc agggggcgca ggaaacgcca
     7861 gccaaacagg tgttacctgc gcacgatcca gattgcttcc tctgcgcagg taatgtgcgg
     7921 gtgacaggcg ataaaaaccc cgattacacc gggacttacg ttttcactaa tgactttgcg
     7981 gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg atcctttgat gcgttgccag
     8041 agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg atcacagtaa aacgttgcca
     8101 gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct ggcaggagca aaccgcagag
     8161 ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca aaggtgcggc gatgggctgc
     8221 tctaacccgc atccgcacgg acagatttgg gcaaatagct tcctgcctaa cgaagctgag
     8281 cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga aatcaccaat gctggtggat
     8341 tatgttcagc gcgagctggc agacggtagc cgtaccgttg tcgaaaccga acactggtta
     8401 gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc tactgctgcc caaagcccac
     8461 gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg atttggcgct ggcgttgaaa
     8521 aagctgacca gtcgttatga caacctcttc cagtgctcct tcccctactc tatgggctgg
     8581 cacggcgcac catttaatgg cgaagagaat caacactggc agctgcacgc gcacttttat
     8641 ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg ttggttatga aatgctggca
     8701 gagacccagc gagacctgac cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat
     8761 atccattttc gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac acaatctctg
     8821 tttgccaacg catttggcta ccctgccact cacaccattc aggcgcctgg ccgcgtgaat
     8881 ttgattggtg aacacaccga ctacaacgac ggtttcgttc tgccctgcgc gattgattat
     8941 caaaccgtga tcagctgtgc accacgcgat gaccgtaaag ttcgcgtgat ggcagccgat
     9001 tatgaaaatc agctcgacga gttttccctc aatgcgccca ttgtcgcgca tgaaaactat
     9061 caatgggcga actacgttcg tggcgtggtg aaacatctgc aactgcgtaa caacagcttc
     9121 ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc
     9181 gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc tttatcatct gccgctggac
     9241 ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc agtttgttgg ctgtaactgc
     9301 gggatcatgg atcagctaat ttccgcactc ggcaagaaag atcatgcctt gctgattgac
     9361 tgccgctcac tggggactaa agcagtttcc atgccgaaag gtgtggctgt cgtcatcatc
     9421 aacagtaact tcaaacgtac cctggttggc agcgaataca acacccgtcg tgaacagtgc
     9481 gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg atgtcaccat tgaagagttc
     9541 aacgctgttg cacatgagct ggacccaatc gtggcgaaac gcgtgcgtca tatcctgact
     9601 gaaaacgccc gcaccgttga agctgccagc gcgctggagc aaggcgacct gaaacgtatg
     9661 ggcgagttga tggcggagtc tcatgcctct atgcgcgatg atttcgaaat caccgtgccg
     9721 caaattgaca ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg tggcgtacgc
     9781 atgaccggcg gcggatttgg cggctgtatc gtcgcgctga tcccggaaga gctggtgcct
     9841 gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa caggtattaa agagactttt
     9901 tacgtttgta aaccatcaca aggagcagga cagtgctgaa cgaaactccc gcactggcac
     9961 ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc
    10021 tgatggactg gggtgcgact ttactttccg cccgtattcc gctttccgat ggcagcgtcc
    10081 gcgaggcgct gctcggctgt gccagtccgg aatgctatca ggatcaggct gcgtttctgg
    10141 gggcctctat tggtcgttat accaaccgca tcgccaatag tcgttatacc tttgacggtg
    10201 aaaccgtgac gctttcgcca agccagggcg ttaaccagct gcacggcggc ccggaagggt
    10261 tcgacaaacg tcgctggcag attgtgaacc agaacgatcg tcaggtgctg tttgccctga
    10321 gttcagatga tggcgatcag ggcttcccgg gtaatctcgg cgcgacggtg caatatcgtc
    10381 tgaccgacga taaccgtatc tccattactt atcgcgccac agttgataaa ccttgcccgg
    10441 tgaatatgac taatcacgtc tatttcaatc ttgacggcga gcagtctgac gtgcgcaatc
    10501 acaagttgca gattctggcg gacgaatatc ttccggttga tgaaggcggc attccgcacg
    10561 acggcttgaa atctgtcgcc ggaacgtctt ttgatttccg cagcgccaaa atcatcgcca
    10621 gtgagtttct tgccgacgac gatcagcgca aagtgaaagg ttacgatcac gcattcttgt
    10681 tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt ctggtcagca gatgaaaaat
    10741 tacagctgaa ggtctacacc accgctccgg ctcttcaatt ctactccggc aacttcctcg
    10801 gcggcacacc gtcgcgggga accgaacctt acgccgactg gcaagggctg gcgttggaaa
    10861 gcgagtttct gccggacagc ccgaaccacc ctgaatggcc gcaaccggac tgcttcctgc
    10921 gtcctggcga agagtattcc agcctgacgg aatatcagtt tattgctgag taatgttaag
    10981 ccctcgtcat gagggcttta tcttcatatt gttcaaatca ccagcaaaca ccgacatatt
    11041 tgcaactcaa tattcacaac aaccttacac tgcgccacta ttttcgctat ggttatgcgt
    11101 aagcattgct gttgcttcgt cacggcaata taatgagaat tattatcatt aaaagatgat
    11161 ttgaggagta agtatatggc tgtaactaag ctggttctgg ttcgtcatgg cgaaagtcag
    11221 tggaacaaag aaaaccgttt caccggttgg tacgacgtgg atctgtctga gaaaggcgta
    11281 agcgaagcaa aagcagcagg taagctgctg aaagaggaag gttacagctt tgactttgct
    11341 tacacttctg tgctgaaacg cgctatccat accctgtgga atgtgctgga cgaactggat
    11401 caggcatggc tgcccgttga gaaatcctgg aaactgaacg aacgtcacta cggtgcgttg
    11461 cagggtctga acaaagcgga aactgctgaa aagtatggcg acgagcaggt gaaacagtgg
    11521 cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag atgatgagcg ttatccgggt
    11581 cacgatccgc gttacgcgaa actgagcgag aaagaattgc cgctgacgga aagcctggcg
    11641 ctgaccattg accgcgtgat cccttactgg aatgaaacca ttctgccgcg tatgaagagc
    11701 ggtgagcgcg taatcatcgc cgcacacggt aactccctgc gtgcgctggt gaaatatctt
    11761 gataacatga gcgaagaaga gattcttgag ctgaatatcc cgactggcgt gccgctggtg
    11821 tatgagttcg acgagaattt caaaccgctg aaacgctatt atctgggtaa tgctgacgag
    11881 atcgcggcga aagcagcagc cgttgcgaac cagggtaaag cgaagtaaac gtcattcgtt
    11941 taaaaaatga gaaagccgac tgcaagtgag tcggcttttt tgtttgtcgt tgctaacaat
    12001 ggagcacatt gcctgatgcg acgctgcgcg tcttatcagg cctgtggtga ttcatcggat
    12061 acgccactct gacggcgcat ccgacaatta aaccttaccc gcgacgcgct tttactgcat
    12121 tcgccagttg acgtaacaga gcatcggtat cttcccagcc aatgcaggca tcggtgatgc
    12181 tcttaccgta ggccagcggc tccccgctat cgaggctctg attgccttcc accagatggc
    12241 tttccaccat cacgccaata atggcctttt cgccaccggc aatctgctgg caaacgtcag
    12301 cacaaacatc catctgcttt ttgaattgtt tggacgaatt agcatggctg aaatcgatca
    12361 tcacctgagc tggcagacct gctttgttca gcccttcttt cacttcagca acgtgcttcg
    12421 cgctgtagtt aggctcttta ccgccgcgca gaatgatatg gcaatcgccg ttaccactgg
    12481 tattcacaat cgccgaatgc ccccatttag ttacggacag gaagcagtgc ggcgcaccgg
    12541 cggcattaat ggcatcgata gccactttaa tcgtaccgtc tgtgccattt ttaaaaccta
    12601 ccggacaaga aagaccagac gccagttcgc ggtgcacctg cgattccgtg gtacgtgcac
    12661 caattgcgcc ccagctcatc aggtcagcga gatactgagg agtgatcata tcgagaaact
    12721 cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag caatttacgg gctatacgca
    12781 gaccgtcgtt gatctggaag ctgttatcca tatgcggatc gttaatcaac cctttccagc
    12841 ccaccgtggt acgcggcttt tcaaaataga cgcgcattac gatttccagc tcatctttca
    12901 gctcttcacg cagcgccagc aagcgagtgg catactcttt agccgcgaca ggatcatgaa
    12961 ttgagcacgg gccaatcacc accaacaggc gatcatcatt acctttcagg atcttatgga
    13021 tcgcttttcg ggcatgagca accgtattcg cggcattttc agttgcgggg aatttttcca
    13081 gcaatgcgac aggaggaagt aactctttga tttctttgat gcgtaaatcg tcgttctgat
    13141 aattcatgtc tgttccagtg tcgccatact tatcttagtg aatgcaatac ttccaatcta
    13201 tatcttccgt cagaatgtgt aaacggggtt ttacactatg aacggattaa tcctagaatt
    13261 agcaagaaaa acgccagatt gtcgcgaaaa acgagatctt tcctacaatt tctaactgta
    13321 attcctttgt ttgttaatta tttcaagatt ctctgctgcg tttcataaac tggctgaaat
    13381 cttaaaccaa tgccttatat tcacctgcaa atgcactgtt ggaagaggtt atccgacata
    13441 acgaccataa caggagcatc ctatgaaaat gacaaaactg gccacattat ttctgactgc
    13501 cactctaagc cttgccagcg gtgccgcact ggctgccgat agcggagcgc aaactaataa
    13561 cggccaggca aacgccgcag ctgatgcggg ccaggtagcc cctgacgccc gtgaaaatgt
    13621 cgcgccaaat aacgtcgaca ataacggggt aaataccggt tctggcggca caatgctgca
    13681 ttcggatggt tcttcaatga acaatgacgg aatgaccaaa gatgaagagc acaagaacac
    13741 catgtgcaaa gatggtcgat gtccggatat caataaaaag gtacaaaccg gtgatggcat
    13801 caataatgat gtcgatacca aaaccgacgg taccacgcag taaccctgca aaatagccaa
    13861 taatgggaga gcggagtctc tcccttttaa tttttcactt ctaaaagcag tatgatgtca
    13921 taaagttaag tgagaataat taagggatga ctcgtcatgg cgcactcaca ctcacacacg
    13981 tcctcacacc tgccagaaga taataatgct cgtcgcttgt tgtatgcttt cggcgtgact
    14041 gccgggttta tgctggtaga agtcgttggt ggttttcttt ctggttctct ggcattgctg
    14101 gccgatgcgg gtcatatgtt gaccgatact gccgccctgc ttttcgccct actcgccgtg
    14161 caattttcac gtcgtcctcc caccattcgc cacactttcg gttggctaag actgaccacg
    14221 ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta ccattttgat tgtctgggag
    14281 gcgatagaac gcttccgcac gccgcgtccg gtcgagggag gcatgatgat ggcaattgcc
    14341 gtggctgggt tgctggcaaa tatactttct ttctggttac ttcatcacgg cagcgaagag
    14401 aaaaacctca acgtacgagc agcggcactg catgtgctcg gggacctgct gggttcggtt
    14461 ggggctatta ttgccgcgtt gattattatc tggactggct ggacacctgc tgaccccatt
    14521 ctctcgatac tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt gaaagatagc
    14581 gtgaatgaat tacttgaagg tgcaccggta tcgctggata tcgctgaact gaagcgtcgt
    14641 atgtgccggg aaatcccgga agtccgcaat gtgcaccatg tacatgtatg gatggtaggc
    14701 gagaagccgg tgatgacgtt gcatgtgcag gtgatcccgc cacacgatca cgatgccttg
    14761 ttggatcaga tccaacacta cctgatggat cactatcaga ttgagcacgc caccattcag
    14821 atggaatatc aaccttgtca tgggccagac tgccacctga atgaaggggt gtctggccat
    14881 tcacatcatc accattaatg ggacagcgcg cgtgagcctc tttcacgtgc gctgttgatc
    14941 cacatccggc tgccgttgag cgcaataaag gtcaggatga tgtactccag agacatggcg
    15001 taaacgccct gaagtgcaaa aataacgacg ctaatcacgt taataatcac ccacaacagc
    15061 cagttttcca catacttacg cgtcatcaga atcattgcca cgatagataa caccatcatg
    15121 catgaatccc agaacgggaa agcgtccggt tgcagttcag gcatcacaac ctgtaatcct
    15181 aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg caaacaccgg attgataaat
    15241 accgtcatca gaccaatcga aacaacgcaa accgccaacc agctgagtgc cttcggcaat
    15301 ggcaaccagc gaattttcaa ctccgcctcg ttctgactgg tttgtcgcga ccacgcatac
    15361 cagccgtaaa tattcgcggc aaagaaaaac acctgtaata gcaggctggc atacagctga
    15421 atctgaaaga aaataatgcc aaacaaggta acgttgatca ggccaaagaa gtagttgctg
    15481 atcttctcca gactggcaag gccaatacac agcaacccgg cgatcgtgcc taccgcttcg
    15541 atccatgaga gatcataacc gcccgcccct attggtatat gtaccaggat attctgcaca
    15601 ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc cttattattc gttatccacg
    15661 tagtgtaacc gcaaaatcca gcatacgatt gagcggcacc agcgccctct ctcgcagcct
    15721 ctcatcgaca tgaacctcgt gattacttcc ttcctgttct aatgcctctg cgatagcctg
    15781 aaggccattc atggccatcc acggacaatg cgcgcagctg cggcaggttg cgccctcacc
    15841 cgcggttggt gcttccagta actctttatc tggcaccgcc tgctgcattt tgtagaaaat
    15901 accccgatcg gttgccacaa taagcctctg atgaggcaat gcttttgcag cagcgatcag
    15961 ttgactggtg gaaccgaccg catccgccat atcgacaata gcttgtggtg attctggatg
    16021 caccagtatg gcagcatccg ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa
    16081 ttcatcatgc acaatgcagg caccctgcca gcataaaatg tctccacccg tctgtttttg
    16141 cacgtaacgc cccagatgtt tgtcgggtgc ccagatgatt ttttcaccca aactatcaag
    16201 atgatcaata agttcgacgg caatgcttga agttaccacc caatctgcgc gcgtttttac
    16261 cgcagcagaa gtgttggcgt agacgacgac agtacgatcg ggatgggcat cgcaaaatgc
    16321 gttaaattct tcaacagggc agccgagatc cagtgaacac tcaacctgaa gtgtcggcat
    16381 cagaattgtt ttttccggac tgagaatttt ggcggtttct cccataaatc tcaccccagc
    16441 gactaacaaa gtagaagcgg gatgctttgc accgaagcgc gccatttcca gagaatcaga
    16501 aatacagcca ccggtttctt ctgccagttg ttgaatttcg ggatcggtat agtagtgggc
    16561 aaccatcacc gcattacgtt cttttagcag acgttttatc ttctcgcggt aatacgcttt
    16621 ttcatcaatg cttaacggcg tcggcttcgg ggggaaagga taaatcgccg tgtctggatc
    16681 aaacattacg ctcatcttac catctcgttt tacaggctta acgttaaaac cgacattagc
    16741 gtaacattcg ctgttatcgg tgtaatttgt tttatatgct aaacaagata gtcgaatggt
    16801 ggggaaaagt cactcgcatt acgcctaatt ccaccgacgt agagttgctt gcacagtaag
    16861 ctctgacgat gtcatcaggt ttacaaagtg aaattgagga tgcctgaata g
//
LOCUS       DAJTYI010000081        16864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYI010000081 DAJTYI010000000
VERSION     DAJTYI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(758..1954)
                     /gene="hemY"
                     /locus_tag="OO564_002596"
     CDS             complement(758..1954)
                     /gene="hemY"
                     /locus_tag="OO564_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6653674.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1957..3162)
                     /gene="hemX"
                     /locus_tag="OO564_002597"
     CDS             complement(1957..3162)
                     /gene="hemX"
                     /locus_tag="OO564_002597"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6653675.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3184..3924)
                     /gene="hemD"
                     /locus_tag="OO564_002598"
     CDS             complement(3184..3924)
                     /gene="hemD"
                     /locus_tag="OO564_002598"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6653676.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3921..4862)
                     /gene="hemC"
                     /locus_tag="OO564_002599"
     CDS             complement(3921..4862)
                     /gene="hemC"
                     /locus_tag="OO564_002599"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6653677.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5249..7795
                     /gene="cyaA"
                     /locus_tag="OO564_002600"
     CDS             5249..7795
                     /gene="cyaA"
                     /locus_tag="OO564_002600"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6653678.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7835..8155)
                     /gene="cyaY"
                     /locus_tag="OO564_002601"
     CDS             complement(7835..8155)
                     /gene="cyaY"
                     /locus_tag="OO564_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6653679.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8203..9516)
                     /locus_tag="OO564_002602"
     CDS             complement(8203..9516)
                     /locus_tag="OO564_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6653680.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9513..10991)
                     /locus_tag="OO564_002603"
                     /pseudo
     CDS             complement(9513..10991)
                     /locus_tag="OO564_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11180..11383
                     /locus_tag="OO564_002604"
     CDS             11180..11383
                     /locus_tag="OO564_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6653681.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11420..12244
                     /gene="dapF"
                     /locus_tag="OO564_002605"
     CDS             11420..12244
                     /gene="dapF"
                     /locus_tag="OO564_002605"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6653682.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12241..12948
                     /locus_tag="OO564_002606"
     CDS             12241..12948
                     /locus_tag="OO564_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6653683.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12945..13841
                     /gene="xerC"
                     /locus_tag="OO564_002607"
     CDS             12945..13841
                     /gene="xerC"
                     /locus_tag="OO564_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6653684.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13841..14557
                     /gene="yigB"
                     /locus_tag="OO564_002608"
     CDS             13841..14557
                     /gene="yigB"
                     /locus_tag="OO564_002608"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6653685.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14641..16803
                     /gene="uvrD"
                     /locus_tag="OO564_002609"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14641..16803
                     /gene="uvrD"
                     /locus_tag="OO564_002609"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6653686.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
BASE COUNT         3903 a         4344 c         4496 g         4121 t
ORIGIN      
        1 tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca agacgtttgg
       61 gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat gatgacgtaa
      121 ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa taatagtgac
      181 aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag cgttttcaat
      241 cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg attattgatg
      301 ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa ttttatcatt
      361 cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa gttattactc
      421 aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg ttatttcccg
      481 attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt cgccttagtg
      541 cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca ctgacagatg
      601 tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc acttgttgtc
      661 atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt atgcatcaga
      721 aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg ttattctgca
      781 acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg tgcagtctgt
      841 ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc agcgccgcgc
      901 ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga cccaatgtgc
      961 tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact ttttccagct
     1021 gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg tcatactggc
     1081 gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat tcaataagat
     1141 gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt tggtttttcc
     1201 accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc aggccaatcc
     1261 atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct ttcgccattg
     1321 atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc tgttccgcca
     1381 gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg ccgtggcgtg
     1441 cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct accggaatgg
     1501 tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt gcttcatcac
     1561 cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt tcagcgtgat
     1621 cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc agtttcagca
     1681 gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac catccacggg
     1741 tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac agcactacca
     1801 tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg tagttgtcgg
     1861 tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact atccccgcaa
     1921 tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg gcgcggctgc
     1981 cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     2041 ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct gcatcagttt
     2101 ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat tttgctggct
     2161 taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     2221 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     2281 tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     2341 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     2401 cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg aactggaaag
     2461 ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     2521 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     2581 tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     2641 gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag aggtcgtgac
     2701 gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg cctgagccag
     2761 cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga cttcatccaa
     2821 ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag cttgttgctt
     2881 aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg ccgtcagttg
     2941 gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac cccagccata
     3001 caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa tcaatgcagt
     3061 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     3121 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     3181 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     3241 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     3301 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     3361 ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     3421 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     3481 gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     3541 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     3601 atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     3661 tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat gcagctgtga
     3721 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct cccccagcgc
     3781 tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     3841 atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc cagcgggaga
     3901 cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc agcgaggatc
     3961 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     4021 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     4081 gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg cacctgacat
     4141 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     4201 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg
     4261 actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc gcgaatgcgg
     4321 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     4381 ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     4441 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     4501 gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     4561 cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac atctttcatt
     4621 gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc ttttacaaat
     4681 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     4741 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     4801 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     4861 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagtgatgtc
     4921 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     4981 ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     5041 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     5101 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     5161 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     5221 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     5281 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     5341 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     5401 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     5461 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc caccgaaagg
     5521 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     5581 ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca
     5641 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gtgtggaagt
     5701 cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca gcctgggggg
     5761 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc gtactgccgt
     5821 gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     5881 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     5941 tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc tttggcagct
     6001 ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     6061 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     6121 cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc gtgttactga
     6181 atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct gcttctattt
     6241 aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc gcgcagtgtt
     6301 gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc tcgataaccg
     6361 tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg acgcgatgat
     6421 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg tctccgccag
     6481 tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag cattgccagg
     6541 taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga atctgacctt
     6601 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     6661 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     6721 ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt atattaaagg
     6781 taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt cgcaccattt
     6841 cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg agattcgcca
     6901 tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca atcaggtggt
     6961 gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
     7021 aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
     7081 cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
     7141 cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg gcttaatccg
     7201 tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca gcacccgcca
     7261 ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct tgttcttcga
     7321 acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg gcgctatttc
     7381 gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
     7441 ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag aaacgcaaga
     7501 cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
     7561 ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact cgtcatcgca
     7621 cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
     7681 tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga ttggcaacct
     7741 gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt cgtgatgagc
     7801 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
     7861 cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg cgatcgcaaa
     7921 tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc agccacacct
     7981 ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag gtaatggtca
     8041 gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg tccagacgct
     8101 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
     8161 taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
     8221 cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga tgtctctgtt
     8281 ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg gggagggcgt
     8341 aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag cgaagcgatc
     8401 gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc gtaattggcg
     8461 gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca agtctggcgt
     8521 atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg ttacatacag
     8581 ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag gttcaatacc
     8641 tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac gccagcgaac
     8701 gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt tgcctgataa
     8761 ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca gtgaatgagg
     8821 tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc catttaaata
     8881 aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt ttacactcat
     8941 aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac tgctgctccc
     9001 ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag agttaccttc
     9061 gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt cggtgcgctg
     9121 tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct gaaagctggc
     9181 aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc agagaataac
     9241 gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat cggaaggtat
     9301 ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc tcatctccgg
     9361 aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca taacaccgtg
     9421 atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa
     9481 caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt gcgcggggca
     9541 ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt gtaatttacg
     9601 ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatcagcg
     9661 gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca
     9721 taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg
     9781 ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc
     9841 tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg
     9901 ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc agactctgtt
     9961 tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat gggttgaaac
    10021 tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt acgccagaac
    10081 actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc atcccacgcc
    10141 agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca ggtcaagagt
    10201 gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg
    10261 ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa tggtacggca
    10321 atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt ctgcgccagc
    10381 acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa attagcgtca
    10441 cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac ctgatgcagc
    10501 gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc ttcaaaatag
    10561 agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac atcgacggga
    10621 ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg accatcgcta
    10681 tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc ggttatcaaa
    10741 tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat taataaattg
    10801 ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt ttctttataa
    10861 gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc aggtgcggaa
    10921 agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca aaccacattg
    10981 cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc agcagaacat
    11041 aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
    11101 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
    11161 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
    11221 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
    11281 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa
    11341 cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
    11401 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
    11461 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
    11521 ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc cgtatgatcc
    11581 tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
    11641 taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga
    11701 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
    11761 ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa
    11821 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
    11881 gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac
    11941 gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata tcggttttat
    12001 gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
    12061 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
    12121 ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc
    12181 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
    12241 atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    12301 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    12361 atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc
    12421 gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga gcaggctatc
    12481 gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac cgccgccagc
    12541 agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggctggt
    12601 gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    12661 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag
    12721 cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    12781 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt
    12841 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    12901 gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca
    12961 ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc ccgataaccc
    13021 tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc
    13081 aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta
    13141 aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    13201 ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg gcaccgaaag
    13261 cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg ctggatattg
    13321 atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg
    13381 gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg gagtccggcg
    13441 aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg
    13501 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc
    13561 tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    13621 aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt
    13681 tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc
    13741 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    13801 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    13861 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    13921 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    13981 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga
    14041 gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    14101 cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta actttgccaa
    14161 atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa
    14221 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    14281 ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    14341 tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg
    14401 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cttgttggat
    14461 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cacatctgga
    14521 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    14581 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    14641 atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc ggtggccgcg
    14701 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    14761 catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat tatggctgtg
    14821 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact gatgggcacc
    14881 agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    14941 caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga agaccagcta
    15001 cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc
    15061 caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa
    15121 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    15181 tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg
    15241 cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac
    15301 gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc
    15361 ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag
    15421 gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat tcgtctggag
    15481 caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat
    15541 aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc
    15601 tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg
    15661 caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg
    15721 cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta cggcgggatg
    15781 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    15841 cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    15901 cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca
    15961 tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    16021 tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    16081 actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga gaagggcgaa
    16141 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg ccagttcagc
    16201 tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca tgcggcgctg
    16261 gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat gaccatgcac
    16321 tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga gggcatgttc
    16381 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    16441 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac tcgccgtctg
    16501 tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc ggaagagtgt
    16561 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcggatgggt
    16621 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg ccacgctaag
    16681 tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg tttgcaggtg
    16741 gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct ggagtcggtg
    16801 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
    16861 taaa
//
LOCUS       DAJTYI010000082        16543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYI010000082 DAJTYI010000000
VERSION     DAJTYI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(111..2552)
                     /gene="rnr"
                     /locus_tag="OO564_002610"
     CDS             complement(111..2552)
                     /gene="rnr"
                     /locus_tag="OO564_002610"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6653687.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2591..3016)
                     /gene="nsrR"
                     /locus_tag="OO564_002611"
     CDS             complement(2591..3016)
                     /gene="nsrR"
                     /locus_tag="OO564_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6653688.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(3221..4519)
                     /gene="purA"
                     /locus_tag="OO564_002612"
     CDS             complement(3221..4519)
                     /gene="purA"
                     /locus_tag="OO564_002612"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6653689.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4623..4820)
                     /locus_tag="OO564_002613"
     CDS             complement(4623..4820)
                     /locus_tag="OO564_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6653690.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4902..5906)
                     /gene="hflC"
                     /locus_tag="OO564_002614"
     CDS             complement(4902..5906)
                     /gene="hflC"
                     /locus_tag="OO564_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6653691.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5909..7168)
                     /gene="hflK"
                     /locus_tag="OO564_002615"
     CDS             complement(5909..7168)
                     /gene="hflK"
                     /locus_tag="OO564_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6653692.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7254..8534)
                     /gene="hflX"
                     /locus_tag="OO564_002616"
     CDS             complement(7254..8534)
                     /gene="hflX"
                     /locus_tag="OO564_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6653693.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8610..8918)
                     /gene="hfq"
                     /locus_tag="OO564_002617"
     CDS             complement(8610..8918)
                     /gene="hfq"
                     /locus_tag="OO564_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6653694.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9004..9954)
                     /gene="miaA"
                     /locus_tag="OO564_002618"
     CDS             complement(9004..9954)
                     /gene="miaA"
                     /locus_tag="OO564_002618"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6653695.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9947..11794)
                     /gene="mutL"
                     /locus_tag="OO564_002619"
     CDS             complement(9947..11794)
                     /gene="mutL"
                     /locus_tag="OO564_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6653696.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11804..13141)
                     /gene="amiB"
                     /locus_tag="OO564_002620"
     CDS             complement(11804..13141)
                     /gene="amiB"
                     /locus_tag="OO564_002620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6653697.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13160..13621)
                     /gene="tsaE"
                     /locus_tag="OO564_002621"
     CDS             complement(13160..13621)
                     /gene="tsaE"
                     /locus_tag="OO564_002621"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6653698.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13593..15140)
                     /gene="nnr"
                     /locus_tag="OO564_002622"
     CDS             complement(13593..15140)
                     /gene="nnr"
                     /locus_tag="OO564_002622"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6653699.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15139..16278
                     /gene="queG"
                     /locus_tag="OO564_002623"
     CDS             15139..16278
                     /gene="queG"
                     /locus_tag="OO564_002623"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6653700.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16261..16314)
                     /locus_tag="OO564_002624"
     CDS             complement(16261..16314)
                     /locus_tag="OO564_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653701.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3753 a         4716 c         4158 g         3916 t
ORIGIN      
        1 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
       61 aagcgcattt tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc
      121 acttttttct tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc
      181 gatggctttt tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca
      241 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg
      301 ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
      361 ggtgcgcgtt cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct
      421 tcgacgcgaa cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc
      481 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
      541 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
      601 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
      661 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
      721 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
      781 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
      841 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
      901 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
      961 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
     1021 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
     1081 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
     1141 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
     1201 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
     1261 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
     1321 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
     1381 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     1441 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     1501 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     1561 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     1621 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     1681 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     1741 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     1801 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     1861 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     1921 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     1981 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2041 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2101 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     2161 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     2221 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     2281 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     2341 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     2401 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     2461 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     2521 gcttcgcgtc cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     2581 aagattttcg tcactccaac agcaataatt tataaagcgg ttgattctct tcaaccaaat
     2641 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     2701 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     2761 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     2821 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     2881 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     2941 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3001 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3061 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3121 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     3181 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     3241 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     3301 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     3361 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     3421 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     3481 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     3541 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     3601 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     3661 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     3721 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     3781 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     3841 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     3901 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     3961 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4021 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4081 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4141 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     4201 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     4261 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     4321 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     4381 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     4441 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     4501 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     4561 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgaacag tctgtttttt
     4621 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     4681 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     4741 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     4801 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     4861 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     4921 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     4981 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5041 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     5101 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     5161 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     5221 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     5281 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     5341 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     5401 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     5461 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     5521 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     5581 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     5641 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     5701 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     5761 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     5821 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     5881 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     5941 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6001 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     6061 cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg gtctaacggc agaaccatca
     6121 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     6181 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     6241 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     6301 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     6361 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     6421 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     6481 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     6541 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     6601 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     6661 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     6721 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     6781 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     6841 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     6901 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     6961 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7021 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7081 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7141 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     7201 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     7261 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     7321 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     7381 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     7441 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     7501 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     7561 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     7621 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     7681 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgct
     7741 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     7801 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     7861 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     7921 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     7981 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8041 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8101 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     8161 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     8221 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     8281 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     8341 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     8401 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     8461 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     8521 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     8581 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     8641 gaagtattct gtgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     8701 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     8761 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     8821 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     8881 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     8941 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9001 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9061 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     9121 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
     9181 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
     9241 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
     9301 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
     9361 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
     9421 cgaaataaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
     9481 tgctgccgca accggatcta cctcctgaag ttgacgatgc aatgactccc aaccttgctc
     9541 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
     9601 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
     9661 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
     9721 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
     9781 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
     9841 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
     9901 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
     9961 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10021 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10081 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    10141 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    10201 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    10261 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    10321 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    10381 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    10441 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    10501 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    10561 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    10621 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cgcggagcta
    10681 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    10741 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    10801 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    10861 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    10921 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    10981 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    11041 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    11101 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    11161 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    11221 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    11281 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    11341 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    11401 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    11461 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    11521 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    11581 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    11641 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    11701 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    11761 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    11821 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    11881 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    11941 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12001 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    12061 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    12121 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    12181 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    12241 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc aatacggaag
    12301 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    12361 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    12421 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    12481 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    12541 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    12601 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    12661 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    12721 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    12781 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    12841 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    12901 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    12961 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13021 acgttatccg tgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13081 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    13141 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    13201 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    13261 ccgggtcagg aagaacacct gtaccttgtt gtggccactc cacaaggcag atggcatcgt
    13321 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    13381 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    13441 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    13501 tacctgcgcc taaatcgcca tacagataga ttacggttgc tccatcgcag gctttcgcta
    13561 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    13621 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    13681 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    13741 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    13801 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    13861 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    13921 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    13981 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    14041 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    14101 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    14161 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    14221 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    14281 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    14341 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    14401 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    14461 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    14521 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    14581 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    14641 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    14701 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    14761 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    14821 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    14881 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    14941 cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    15001 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    15061 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    15121 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    15181 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    15241 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    15301 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    15361 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    15421 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    15481 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    15541 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    15601 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    15661 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    15721 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    15781 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    15841 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    15901 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    15961 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    16021 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    16081 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    16141 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    16201 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    16261 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    16321 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    16381 tatttatcaa taggttaaaa aataccattc accgtgcgaa gtttctttga aattaagatt
    16441 acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    16501 caataacgca tcgcgtcgct gtggatattt tattgagaga aga
//
LOCUS       DAJTYI010000083        16467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYI010000083 DAJTYI010000000
VERSION     DAJTYI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(18..254)
                     /locus_tag="OO564_002625"
     CDS             complement(18..254)
                     /locus_tag="OO564_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6653702.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(372..1811)
                     /gene="rsmF"
                     /locus_tag="OO564_002626"
     CDS             complement(372..1811)
                     /gene="rsmF"
                     /locus_tag="OO564_002626"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6653703.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(1891..4389)
                     /gene="yebT"
                     /locus_tag="OO564_002627"
     CDS             complement(1891..4389)
                     /gene="yebT"
                     /locus_tag="OO564_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6653704.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4358..5642)
                     /gene="yebS"
                     /locus_tag="OO564_002628"
                     /pseudo
     CDS             complement(4358..5642)
                     /gene="yebS"
                     /locus_tag="OO564_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="OO564_002629"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="OO564_002629"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6653705.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="OO564_002630"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="OO564_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6653706.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="OO564_002631"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="OO564_002631"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6653707.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="OO564_002632"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="OO564_002632"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6653708.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11624)
                     /locus_tag="OO564_002633"
     CDS             complement(10251..11624)
                     /locus_tag="OO564_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653709.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            11801..12592
                     /gene="kdgR"
                     /locus_tag="OO564_002634"
     CDS             11801..12592
                     /gene="kdgR"
                     /locus_tag="OO564_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6653710.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12735..12974)
                     /locus_tag="OO564_002635"
     CDS             complement(12735..12974)
                     /locus_tag="OO564_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653711.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13133..13276
                     /gene="mgrB"
                     /locus_tag="OO564_002636"
     CDS             13133..13276
                     /gene="mgrB"
                     /locus_tag="OO564_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6653712.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13351..13638
                     /locus_tag="OO564_002637"
     CDS             13351..13638
                     /locus_tag="OO564_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6653713.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14308..14451
                     /locus_tag="OO564_002638"
     CDS             14308..14451
                     /locus_tag="OO564_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6653714.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14464..14673
                     /gene="cspE"
                     /locus_tag="OO564_002639"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14464..14673
                     /gene="cspE"
                     /locus_tag="OO564_002639"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6653715.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14839..15648
                     /gene="rlmA"
                     /locus_tag="OO564_002640"
     CDS             14839..15648
                     /gene="rlmA"
                     /locus_tag="OO564_002640"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6653716.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15645..16211)
                     /gene="mntP"
                     /locus_tag="OO564_002641"
     CDS             complement(15645..16211)
                     /gene="mntP"
                     /locus_tag="OO564_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6653717.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4316 a         4003 c         4187 g         3961 t
ORIGIN      
        1 gacgggttct ttttggatca ggcaagacgc ataatcctgg catcagattg ttgatcgtaa
       61 cgggtacggt aaaggacaga gtgttcgcca tttagcaggg cagggatact ggtttcagca
      121 tcccaggcat ccagttgcat acataaatcg gggtcagatt tacaaggaat ggttaacgtt
      181 cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc gcctatgcgc
      241 acactggttt tcatcttcgt gctctccatt tagctgctga ttgtggtacg accagtccga
      301 tcgcacaatt tttcagccta gttgaggccg ccgaatgtgc cagtcaaaaa gtgcgctttt
      361 tttgtgcgct gtcatgcgtt actggtaaaa agtttcccgt cccgcaccag ttcacgcggg
      421 tagctgtttt tcagtcgcga accaatccgt ttggctaaac caatcggctg atgctggaaa
      481 gtaaccaaca cgtcatccgc cactggcgcg gcttgcgggt aaacatcgcg cccgcgatac
      541 cactcctccg cttcctgcgg tgtcagttca aaagcgttta cattgtcggg ggaggcatgg
      601 gcaataaccg cttcatgctg ccagcgataa cctttgttat gcgtttcggc aagtttaatc
      661 cctaagcgag aaaatcggac tttaccgatc agggcttcaa tgcctaccgg gaacaaccac
      721 agttctttgt cgcgttgcca gagtcgcagg tttccatccc agtttaagcc aacacttgca
      781 gccgcctgac gaatttgtcc ggcttcgcga tctttcaccg ggctgaacgg gaaattgccc
      841 actttgtatt ttggggcggg taaggcgggg atcgcctgag ttttacgcag acgagcaacg
      901 aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaaaccttc ttcggtcagc
      961 gccttgtttg caccagggaa gagatcgcca agtggtaaaa actctacttc gtcggggtaa
     1021 gtctctttca gccaccggca aacggcttcg ttttcttccc ggtttaaggt acaggtcgag
     1081 taaaccagcg taccgccagg acgtaatgca tgaaaggcgc tgtcaatcag ttcccgttgg
     1141 gtagctgcga tttcctgatt gctttcgggt gaccagtttt ttagcgcatc gggatcttta
     1201 cgcaccacgc cttcgccgga gcagggagcg tccagcaaaa tggcatcaaa catttctggc
     1261 actgacgcac caaacacgcg gccaccaaaa tgtgtgagcg caacattact gatgccacag
     1321 cggctgatat tggcatgtaa cacttttacc cgactggcgg aaaactcatt ggcaaggatt
     1381 gccccttcgt tattcatccg cgcggcaatt tgcgtcgttt ttgagcctgg cgcagcagcg
     1441 acatccatca cccgctgtgg tgcattaccg tcagcaaaca aggcggcgac gggcaacatt
     1501 gaactggctt cctgaatata aaacaggcca cttaaatgct cggtggtact acccaatggc
     1561 aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg aattggcgta
     1621 agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat tttcagcgta
     1681 ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc atcaaatgag
     1741 agcgtcgaag gcatagcttc gcgcatttgt gtcagaaacg cgtccgggaa ataaacggcg
     1801 tgttgggcca cgagcatgta ccactggcaa aaaaacaggc gcgcagttta gcataaacgc
     1861 tccggcgcag gcacgccgga gcagtgggca ttatttggga agcgcagttc cccattcacg
     1921 ccactctttc ggttcacttt cctgcaacag gaagtgtttg ccttcctggg ctttcggtgc
     1981 aagtggcgta cccggaggcg tggcgaaggc gatgccgcca cggataaatt ggttaaaggt
     2041 gccggttttc actacgccgc ccgtcagacc aaagtccaga ctgtaacccg atgccaacca
     2101 gaagacggaa ttgttacgca ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat
     2161 cacgcgatct gacaatgtcc ccagcgtcat tccggtaacc gtacctactt ccagaccacg
     2221 gaacagcaca ggcgtaccga tgcctaacga accggcttcc ggcgcttcaa caataatgct
     2281 taaaccatct aggtaacgcg aatcagtaat ggtggcctct tctaattcaa agtcgcgacg
     2341 aggattgcca cggcctggtt cgacgttgat atacggctgg aggatagtat ctagatgctc
     2401 aacgccagct gccgagattt gcggtgtgac cactgagaag cgcgtaccac cgcgagcaaa
     2461 ggtctggaca tattccggat agagcaccgc ctttgcctgt acttcattgc gtgcggtaat
     2521 cagatccagc gtctggattt gcccgatatc aataccgaga tagcgaattg gcatcccgac
     2581 cgccagtttt ccggcatcga aagcgtgaag cgtaatctgc ctgccaaccg cacgggccgc
     2641 tgtttcggaa gcatacagaa ttcgcttgtc gcctttacgc tgactggcgc tggcaccact
     2701 gaggttatcg aagctaatgg ccccctttaa tgctctggag agcggggatg cctgtacggt
     2761 caggccacta ccattcagct gaactttcgc cccgccttct gcccagaaca cgctgttgct
     2821 ggtcaaaagg ttgcgatatt ccggtttaat gtgcagatcg atatcgaacg cgttagctcg
     2881 cggacgcacg gtaataactt caccaacttc aaatttacgg tacagcacta ccgatcctgc
     2941 ctgcacatcc ggcagcgtct ctgcactcaa actcacggtt gtggtgggta aatcgctaag
     3001 gctgttctcc agcgcttttt ccagattggc atacagtgga tagctggctt tcatctcgcc
     3061 tttatcgccc ggcagaatac gtatcccgcc gttaatccat tctgaggcgc tggcaccgag
     3121 aaactcaacg ccatccagcc ccaccttcac gtcgacacgg ctgttgacga caaatttgct
     3181 atcgcctttt accagttctc gatgctgagg ctcgatggcg acggtaaagg tgacgccttt
     3241 gctggtgagt ttacgatcga taacctggcc tacctgcacg ccgtgaagaa tgagcggctg
     3301 acccgcatca ataccgtaac tttccggtgc tgtcagggtc agtgtcagaa cgtccggctc
     3361 ttgcagcagg gctttttcgc ccggcacaac aacgaactct ttgcgtggct cgccatcgcc
     3421 gggcaccaac tcaaaggttt ttccggtcag cagggcgctg agattagcat cgctaaggga
     3481 taatttcggg ttgcgtaatt ctatgcgggt attttcacga agcagggtaa cgacgctggg
     3541 atcaacggtc atttccccag tgactttacc accaggattt aaatccagtt tagtcagctg
     3601 tccgacttcc agcccctgat acattaacgg cgtcgagtcg gcggttaatc ctgctccact
     3661 cggcagttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa cgccggaaac
     3721 gttccagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc
     3781 aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat
     3841 cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agatcggggg cttgcaggtg
     3901 gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg cgacaaagtg
     3961 atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga cgagggcgtc
     4021 cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact gagtctcttc
     4081 gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc
     4141 gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt
     4201 acggcccgga acaataccat ccgccgacat aaagtcgatg gtgatggtat taccccggtc
     4261 ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag gcagcagcca
     4321 gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc
     4381 ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca
     4441 aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat
     4501 cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgtc
     4561 caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa
     4621 aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac
     4681 tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac ataattccgg
     4741 aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata ggcaataggt
     4801 tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat
     4861 gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc tgatcaggat
     4921 agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc
     4981 gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac gtcaccgtcg
     5041 ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata tgggcataat
     5101 cctgtacctt atatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagtc gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accattgatc ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcaa tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcataagca acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa
     5761 gtgattaaat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcacagcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttctcgac atcgatagta ccgtcttcgc tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctcgccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 acaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgat ggcgtttgcg
     7741 cgtgaaatcg atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccactgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg
     8761 aaatcaggag atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg
    10261 gctcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc
    10381 agcgccgcgc cactactctg acccagtaga cgagccgttc ctaacatgcc actggcccca
    10441 ccgctacgtt cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccagggaaaa aagccccgca gccatgatga acaaccctaa agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt
    11221 tgtggataga tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa
    11281 ccttgtatga cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc gcagaacagt
    11341 gaagacaaca gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatcg
    11401 cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc
    11461 cagatggaac tggctggtgt ggcatgaagg tccgtggcga ttgttggcag ggcgacgttg
    11521 gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa tatcgcaccg
    11581 tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt
    11641 tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa
    11701 ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa
    11761 cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga
    11821 taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg
    11881 tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac
    11941 cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc
    12001 ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt
    12061 cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac
    12121 tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat
    12181 gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat
    12241 tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt
    12301 ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct
    12361 ggaccaggtg cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg
    12421 atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc
    12481 cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac
    12541 cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta
    12601 agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg
    12661 aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg
    12721 gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa
    12781 tctgttatcc ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg
    12841 gccgtcagat aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa
    12901 acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga
    12961 atgatgaatc gcattacaat ctcttctctt tttatgttcg cttaatcgta gcggcaatat
    13021 gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt
    13081 taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa
    13141 gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt
    13201 attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca
    13261 gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt
    13321 agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg
    13381 aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt
    13441 tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg
    13501 gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag
    13561 aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc
    13621 ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact
    13681 caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc
    13741 tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt
    13801 tcctcattaa tttattagga tgtttacatc agatttgtga ttaagcgtgg tattatttat
    13861 tagcgaaacg tttctctctt gatttttttg ctcactcatc aatttttctt attttaaatt
    13921 tacaatccct ttggggattg acttctcttt agggtaattg atagccgtta actgactgtt
    13981 ttatgagaaa aagtgatata actttttatt cattgcatag cagaaaatgt gatattgcac
    14041 gcactatgta ataacttctc cctctggcct ggaacaactg aacttattga actatgttag
    14101 aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
    14161 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
    14221 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taaaagattt
    14281 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctaggt
    14341 aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
    14401 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
    14461 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
    14521 attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat
    14581 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
    14641 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
    14701 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
    14761 cgctcctggc gttcggatat ttgccagcca cgtgattcgc gttacacttg cggcctttag
    14821 tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt
    14881 gaaaaaaaca gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat
    14941 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
    15001 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
    15061 gtcgcccaac tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt
    15121 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc
    15181 ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt
    15241 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
    15301 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
    15361 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
    15421 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
    15481 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
    15541 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
    15601 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
    15661 gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt
    15721 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag
    15781 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca
    15841 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
    15901 ccgtgccgac ggcgcggctc ttcatcttca tcatctgagc cacgaaaacc ctcaataatc
    15961 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca
    16021 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
    16081 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag gggggcacct
    16141 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
    16201 gtgatattca tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
    16261 aacatgcata catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat
    16321 tgatgtgcat atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca
    16381 acgagtatgt tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg
    16441 cgcaacctta acatattttg agaatat
//
LOCUS       DAJTYI010000084        16434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYI010000084 DAJTYI010000000
VERSION     DAJTYI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..536)
                     /gene="kptA"
                     /locus_tag="OO564_002642"
     CDS             complement(<1..536)
                     /gene="kptA"
                     /locus_tag="OO564_002642"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6653718.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAV"
     gene            785..1468
                     /locus_tag="OO564_002643"
     CDS             785..1468
                     /locus_tag="OO564_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6653719.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1465..1926
                     /locus_tag="OO564_002644"
     CDS             1465..1926
                     /locus_tag="OO564_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6653720.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1939..3111
                     /gene="iadA"
                     /locus_tag="OO564_002645"
     CDS             1939..3111
                     /gene="iadA"
                     /locus_tag="OO564_002645"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6653721.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3176..4087
                     /gene="hypT"
                     /locus_tag="OO564_002646"
                     /gene_synonym="qseD"
     CDS             3176..4087
                     /gene="hypT"
                     /locus_tag="OO564_002646"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6653722.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4080..4472)
                     /gene="iraD"
                     /locus_tag="OO564_002647"
     CDS             complement(4080..4472)
                     /gene="iraD"
                     /locus_tag="OO564_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6653723.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4469..4552)
                     /gene="idlP"
                     /locus_tag="OO564_002648"
     CDS             complement(4469..4552)
                     /gene="idlP"
                     /locus_tag="OO564_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6653724.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5145..5975
                     /locus_tag="OO564_002649"
     CDS             5145..5975
                     /locus_tag="OO564_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6653725.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAVLLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6116..6889)
                     /gene="uxuR"
                     /locus_tag="OO564_002650"
     CDS             complement(6116..6889)
                     /gene="uxuR"
                     /locus_tag="OO564_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6653726.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7104..8564)
                     /locus_tag="OO564_002651"
     CDS             complement(7104..8564)
                     /locus_tag="OO564_002651"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6653727.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8645..9829)
                     /gene="uxuA"
                     /locus_tag="OO564_002652"
     CDS             complement(8645..9829)
                     /gene="uxuA"
                     /locus_tag="OO564_002652"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6653728.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10169..11512
                     /gene="gntP"
                     /locus_tag="OO564_002653"
     CDS             10169..11512
                     /gene="gntP"
                     /locus_tag="OO564_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6653729.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11763..12665)
                     /gene="fimH"
                     /locus_tag="OO564_002654"
     CDS             complement(11763..12665)
                     /gene="fimH"
                     /locus_tag="OO564_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6653730.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12685..13188)
                     /gene="fimG"
                     /locus_tag="OO564_002655"
     CDS             complement(12685..13188)
                     /gene="fimG"
                     /locus_tag="OO564_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6653731.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13201..13720)
                     /gene="fimF"
                     /locus_tag="OO564_002656"
                     /pseudo
     CDS             complement(13201..13720)
                     /gene="fimF"
                     /locus_tag="OO564_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13730..16366)
                     /gene="fimD"
                     /locus_tag="OO564_002657"
     CDS             complement(13730..16366)
                     /gene="fimD"
                     /locus_tag="OO564_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6653732.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
BASE COUNT         3984 a         4231 c         4019 g         4200 t
ORIGIN      
        1 acagcaaccg ttgatgtcag ccagaccccg ttttccgctt gccagaaagg gatgcctcgt
       61 ttcgccattt cctgtgcttt gacggttaag acaaccggcg atccgtgtct tgctcccact
      121 ttgcgtgccg ttgcttcatc agcagagaga tgcacgtaat gacgatcccc tgcaatcagc
      181 ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac cgtggtagag aaactgtggc
      241 ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg aatgcccctg aaccgcgcgg
      301 atgcacctac catcactgga ataactaaaa cgctttttat cgctggttgc gaccacggta
      361 tccagcaatg cacgggttaa tcgcttgccc gctttttggg cacaaagtat gagcttattg
      421 atatccgccc atccttcacg gtccagtacg ataccaatcg cctccggttt gtgtcggagg
      481 acaaaactta aaaacttgct cgtatccgcc agctcttttt cgttgtactt tgccatcgcg
      541 ttttcgtccc tggattttct gtttttattt actaatgtca ttgtcactgg gtttatgaat
      601 tttctgcaac tcaatcctgc gcagaacctt ttccacacag cgtaattttt cccctatgca
      661 aaaaacgcat agccagaaac atacttgcat tttccccata cccacatggc tgataaggtt
      721 tttcagttcc aggaattttc ctggctcaag caacacacaa acacaaaaaa caatggggat
      781 tgttatgaca caacaagggg atgctgtagc cggggagctg gcgacagaaa aagtgggcat
      841 taaagggtat ctggcttttt ttctcaccat aatctttttt tccggtgttt tttccggaac
      901 agacagctgg tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc
      961 tggtgcgaat ggagccacca cctcatttcg cggggtgggt ggcgcggggg ctaaagatgg
     1021 ctttctcttt gcgctggagc tggcaccgtc agtcattctc tctctgggga ttatttcaat
     1081 taccgatggt cttggtggat tacgcgccgc gcagcagtta atgacacctg tgctaaaacc
     1141 gttgttgggc attccaggga tctgctcgct ggcactgatc gctaacctgc aaaacacgga
     1201 tgccgccgcc ggtatgacca aagagctggc gcaggaaggt gaaattaccg aacgcgataa
     1261 agtgattttc gccgcatatc aaaccagcgg tagcgccatc atcaccaact acttctcttc
     1321 cggcgtcgcg gtgtttgcct ttctgggcac gtccgtaatc gtacctttag ccgtcatcct
     1381 ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg
     1441 caacccaacg caaggagcgc aagcatgaca actcaggtcc gcaaaaacgt catggatatg
     1501 tttatcgatg gcgcgcgccg tggctttact atcgccacga ccaacctgct gccaaacgtg
     1561 gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg
     1621 catatctgtg aacccgtaat ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt
     1681 ttagccgcat taatgagtat gggcggcgca gtcggtgttg ccgccagtct ggcaaccgct
     1741 gacgcattaa ccggccacga tgtcaccgtt ttactgcccg caatgtattt aatgggcaac
     1801 ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa atattacccg
     1861 catatcatta cggtctgcgt gattaacgca ttgctgtcga tctgggttat gcagcttatt
     1921 gtttaacaag gagttaccat gattgattat accgcagccg gttttaccct gctgcaggga
     1981 gcgcatttgt atgcgccgga agatcgggga atttgcgatg tcctcgtcgc taacggcaaa
     2041 attatcgccg ttgccagcaa tatcccttct gacattgtac cgaactgcac ggttgtcgat
     2101 ctcagcgggc agatcctctg cccaggtttt attgatcaac acgtccattt gattggcggt
     2161 ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg cgctaagtcg cctgacggaa
     2221 gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact ctatctctcg ccacctggaa
     2281 tccctgctcg ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc
     2341 ggcgcttatc atgtcccttc ccgcactatt acgggttccg tggaaaaaga cgtggcgatt
     2401 atcgatcgtg tgattggcgt gaaatgcgcc atctctgatc accgttctgc cgcaccggac
     2461 gtttatcacc tggctaatat ggcggcagaa tcccgcgttg gcggtttgct cggcggtaaa
     2521 cctggcgtca ccctgttcca catgggcgac agtaaaaagg cgttaaagcc tatttatgac
     2581 ctgctggaaa actgcgatgt gccgatcagc aagctgctgc cgacccacgt taaccgcaac
     2641 gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag gcggcaccat cgatatcacc
     2701 agcagcattg acgaaccggt cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt
     2761 attctgctgg cacgcgtcac cctcagctcc gacggcaacg gtagccagcc gttcttcgat
     2821 gacgaaggga atttaaccca tatcggtgtt gcaggttttg aaacgttgct ggaaaccgtg
     2881 caggtgctgg tcaaagacta tgatttcagt atcagcgatg ccctgcgccc gctcaccagt
     2941 agcgtagccg gtttccttaa catgagcggg aaaggcgaaa ttctgccagg caatgacgca
     3001 gacttactgg tcatgacgcc agaattgcgc attgagcagg tatacgctcg cggcaaactg
     3061 atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc
     3121 agccgcccta atggacgtag aatgccccca agggcggctg acagagtaaa acgtaatgga
     3181 tgactgtggt gcggttttgc ataatattga aaccaaatgg ctttatgatt ttctgactct
     3241 ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt
     3301 cagccgccgc atccgtgcac tggaacaggc gattggcgtt gaattgttta accgccaggt
     3361 gacgccactg caactctcag aacaagggaa aatcttccat tcgcagatcc gccatctgtt
     3421 gcaacagtta gaaagcaacc ttgcagagct gcgtggcggc agcgattacg cgcaacgtaa
     3481 aatcaagata gccgctgcac actctctttc cctcggtctg ttaccgtcca ttatcagcca
     3541 gatgccgccg ctctttacct gggcaattga agccattgat gtcgatgaag cggtcgataa
     3601 actgcgtgaa gggcaaagtg actgtatttt ttcctttcac gacgaagatt tgctggaagc
     3661 gccgtttgac cacattcgct tatttgaatc tcaattgttc cctgtctgcg ccagtgacga
     3721 acacggagaa gcacttttta acctcgcgca gccacacttt ccgttactga attacagccg
     3781 caactcctac atggggcgat tgattaatcg caccctgacg cgccacagtg agttaagttt
     3841 cagcgccttt tttgtctctt cgatgagcga gcttttaaag caggttgccc tcgacggctg
     3901 tgggattgcc tggctgccgg agtatgccat ccaacaagaa attcgcagcg ggcagctcgt
     3961 tgtgctgaat cgggacgaac aggtgatccc gattcaggcc tacgcatacc ggatgaatac
     4021 ccgcatgaat ccagttgccg aacgcttctg gcgtgaactg cgcgagctgg agattgtgct
     4081 tagctgacat tctcaagcgt cgcactgcgc caaccgccat tatcccagac cagatcaaac
     4141 gttagcggtt catcgtcgtg gtacagcatt atctcaaggc tgatcgccag agtgtggcag
     4201 tacgcttctt tcaaattaac ctgcaacgcc gcaatccgtg gctcatgaaa taacagtgca
     4261 gaacgcagct gacggcaata ccagctctgg agatgtttgt cgctcgggtt atgcacttgc
     4321 cactcgggca tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga attaaaaaga
     4381 gtaaggagat cgctgcatac cgatttacgc agcaatgacg ttttattgcc tgaaatttca
     4441 ggtaaaacag cctgaagtga ttggcgcatc attttgcgca ctcctgacgt ttagcaacat
     4501 atgcggcttg ccctgaacac cgggcaccac tgtattgatg ctcattttcc atagccattc
     4561 ccttttgttc attacatcca tgtcagtctg agacaggcct gaatcctgtc agcaaagtat
     4621 aaaaccttac ttgcctatga atatctactg acttttcagg aaaaaagtca tgatttgata
     4681 aaaatcatca aactcaaaaa aaaaccgcta gtatattctg aatattccga atagttacgt
     4741 agatgaaaat acttgtcacc gctgctactt aattccggtg ggaaattatt tatttccgca
     4801 taatcagcca tgtattattt gaaacaatta gtttgtaaat aatataaatt gataacatat
     4861 caaatacatc aaaaatacca ttcacaggca ttattaatat agacctcaat atagcaacat
     4921 caaattccaa ttagtaataa gatatgcttt taaattgcat gcaaataatt ttaaatgagc
     4981 gttttttgat actttatagt gtttcctacc ctttaatatc actgtgtaga gttgatatca
     5041 ctacaacaga aacatgcatg tcatttgttt acttctcttt ttactacaga aaaatttatc
     5101 tatttttcta aacatcagtt aaatactcat aaaaggtaac tattatgtct atgccattaa
     5161 gcaatgccgc taataaccaa ttcaatacaa ataatcactt ccttcatcat ccaaaagtcg
     5221 acagtgagtt aacacgtcga tatgaatatg cacgcctgga tacggaaaat atctatcttc
     5281 tgcctcttgc tcgtggcaat aaccataatt acgatggaaa aagcgttgtc gaaattagaa
     5341 aacttaatat tggaaaacag tcctggccat ttaattatgt gacagataca tgcagagaat
     5401 tcaatggtat tacaacaaca ggacgtatgc tctatcgaaa cttgaaaatc acctcagctt
     5461 tagacgaaat atatggtggt atatgtaaaa aggcgcatgc tgctacagag ttagcagaag
     5521 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
     5581 atgaaataac attaggccct ggttgcaatg ttcctggtta tgccggaacc acgataggat
     5641 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaagaattg
     5701 atatctatat tgatcagata ataactgtct caggggtagc aaattcttcc ggattcgccc
     5761 ttgccgtatt acttaatgct aatattgaga tgggaaatga cccaatcatt ggtatagaag
     5821 cctatcctgg taccgcggag atccattcaa aaatgggata taacgttatc cccggtgatg
     5881 aagatgcgcc actcaaacgc atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
     5941 taaaaaatgg tgaatggaat tatattggta aatagccact gagaagaatg agtactaata
     6001 ttcaggttat acagatattt aaacaaaacc ctgcaattta caaagtaata ctcattgcga
     6061 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
     6121 cgtcgacttt atccagcggc catgaatcaa agagatagcc gtcaaaataa atatcgtcaa
     6181 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
     6241 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     6301 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
     6361 tttcccgcca ctgccaggac tgacggaata actccaccag catgctgtta tgcgttgctt
     6421 cagcaatggc gagatggaac tgcatgtcac cgctttcgct gctacccggc gcgctggaag
     6481 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     6541 cctgcagtgc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     6601 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
     6661 agctatcaag cacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     6721 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
     6781 gctccggcgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgcggatca
     6841 tcgccccgac ttcctggtaa ggtctttgca cagaggtgac agatttcatg gtgttgtgtc
     6901 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     6961 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
     7021 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     7081 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     7141 cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt cggcattctg cggcagatca
     7201 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     7261 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     7321 tcatccacgc cctgggtgta acgcatccag ccagccacgc ccagcgtcag gtgacgccag
     7381 ctgccgccgt tttgcaggtg cagacgcacc gggtccagta gacgctgcgg taacttctgg
     7441 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     7501 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     7561 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     7621 gcaatggttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     7681 agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     7741 ttatcccagt ccgggcgacc attaacgaag ttatcttcaa tcacccactg acggaacggt
     7801 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     7861 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     7921 tcaatccatg ccgctagttg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     7981 gccacatgac cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     8041 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     8101 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     8161 tcagcgcagt agcctttttc cgtgaccgtt agagagacaa tcgccgtttg cggacgcgcc
     8221 atcgcgttga gaataccttc gcagccgtcg atttccggat gcagcgcttc tttcatcgaa
     8281 ccgataattt tcagctcggt gctttctgcg cctttttccg ctacggtgta cagcagttgc
     8341 tgttttttca ggttttcgat cagcatacgg tcgttgcctg gcatcaggtt aacttcgcag
     8401 atgccccagt cgctgtcggt gctttccagc agatgatggg tatacaacgc ctggtgcgcg
     8461 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     8521 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     8581 cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     8641 ggtattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     8701 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     8761 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     8821 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     8881 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     8941 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     9001 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     9061 tccactgcat atcttcaatg gtggaaacaa tgcgcggcaa gccgaggatc gggcgcggcg
     9121 gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc agcaactgga ataatcgctt
     9181 tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagctcca
     9241 ggtgcttacg gaactggtcg agggtatacc cttcttccgc gcccggcaga ccggcaatga
     9301 tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt tcagctgcct
     9361 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     9421 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     9481 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     9541 tgccacactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     9601 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     9661 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     9721 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     9781 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     9841 tttcacatag tgggttccac agcaaagaga cgcggaacct tggagttgtt taggtatccg
     9901 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     9961 cttttggtaa gacatgtcaa cccgaactct gaaattggtt aaccacatca caagaatttc
    10021 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
    10081 atgtgacatt catcgcaaca atggttgacc aatttgcatg acatgtcgcc caaaataaca
    10141 acggttcaac caagtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
    10201 cggcattggc ctgatgctga tactgaattt gaagttcaaa atcaattcaa tggtggcttt
    10261 gttggtggcg gcgctgtccg tcgggatgct ggcaggcatg gatttgatgt cgctgctgca
    10321 caccatgaaa gcgggcttcg gcaacacgct gggagaactg gctatcatcg tggtgttcgg
    10381 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cacatacgct
    10441 gctggcgcgt ctaggtctgc gctatgttca gctgtcggtg attatcatcg gccttatttt
    10501 cggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
    10561 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
    10621 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
    10681 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
    10741 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
    10801 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
    10861 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
    10921 agatacccct gcctgggaag tggttaactt tattggttcc tcgccgattg cgatgtttat
    10981 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
    11041 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
    11101 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
    11161 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
    11221 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
    11281 aatccttgat ccagcaaccg gtcagctggt tggcgtgaat ccggcgctgc tggtactggc
    11341 gacggctgcg ggttccaaca ccctcaccca cattaatgat gcatctttct ggttgttcaa
    11401 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
    11461 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
    11521 cagagcctgc caatggcagg ctcagactga tgactaaagt ctaaaaatgt gcccggacag
    11581 tcccctcgcc cctccgggga gagggttagg gtgaggggaa cagaccagta aagtgcgctt
    11641 tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag
    11701 gttttgtagg tctgatagcg tagcgcctca ggtaccagca ttagcaatgc gctgtaattt
    11761 ttttattgat aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc
    11821 cctccggtac gtgcgtaatt tgccgttaat cccagactta ccgccgaagt tcctactgct
    11881 cctaacgata ccgtgttatt cgttggaata atcgtaccgt tgcgcgtcaa ctgtacgccg
    11941 acgccctgcg ctggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg
    12001 gttgtgccgg agaggtaata ccccaggttt tggcttttcg cacaataaac ggtaagagga
    12061 attggcactg aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatca
    12121 caaccgccag tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa
    12181 tcatcgctgt tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct
    12241 ttaatcgcca ccccgcccgc actgctcaca ggcgtcaaat aaagcgccac cggccacggc
    12301 ttatccgttc tcgaattata aacaacccgc ggcgtttcgc tggtggtcgg aaatggatag
    12361 ctactgccac tatattttac ggtcccggaa aaattagata acacgccgcc gtaagccgag
    12421 cctcgttgca gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt
    12481 tgcgtcgaaa gatctacgac caggttttgc cccacattca cgacaggcgc aaggtttaca
    12541 taaacattag cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg
    12601 aatgaccagg catttaccga ccagcccatc agcagtacag caaacagggt aatagctcgt
    12661 ttcattacaa ttatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg
    12721 aatggttccc tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg
    12781 cgctgattgt gaggaatcat ccacctgaac tgttttggtt gcgccagtat tcaatgtgtt
    12841 gccactgtca tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata
    12901 tccggtgctg tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca
    12961 attagtcaac tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact
    13021 gaaagaataa agatcgccga gatctaccgt ggcattggtg gtggaaaccg tacacggttt
    13081 ggcgacgacc ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc
    13141 gagcgccaac attgccgcca atacataccc acgtttgcac catttcatga gcatctccag
    13201 ttactgatat tcaagagtga aggtagccgt agcattgata tgccccgcag tgacaggcac
    13261 ctgtgtcgcc attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt
    13321 cgtccacgaa atcgcagacg atggagcatt aaggggtatt tgattttgct gctcattcag
    13381 aagctgtatt cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc
    13441 attgtggcta tctgcaacgc cggtaaaccc aacttttacg gcagaaacag ggtgacagca
    13501 aaatacgaaa tggaacgaca ggagtcgtcg cgccaatgtt gttaaattgc ttcgccgcgt
    13561 tttccatcag atcaacagta aaattggttg attcagcggc cacactacag ccgttatctc
    13621 tgacatagcc gcggatagta atcgtgctat ccgcagccaa aacgcgactt accgccagcc
    13681 acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat cacgccccct taacgacatt
    13741 cagctgatag ctgggttaat aactgctgct gactctctgg tggcagttga taattggcga
    13801 cacagtgagc attttcctct tctccccatt tcacctgaac ttttcccgct aaaggcattc
    13861 cgctgaggta aaactgacca ttatccgcaa caatgccgct actctggcta ctctctgatg
    13921 tcaccatcgc cccaaacggc agcggcttat tattgtgagt tagcgtcatg agcagtttta
    13981 tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc acgagtggga acaacgttag
    14041 cgaccgcgtt atctaaatcg acgttatcag ccaaggtatt ggtatccagc gccactctat
    14101 tttcccgata ttcagtggca taaggcagca cggcataacc acgccagtcg gtacgcaccc
    14161 ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc tttaacaagc accaccgtat
    14221 cgtttaacgg ctgccccagc gttacgccat tggcatgagc caatacccca ccgctgactc
    14281 cgtaatagag ctgcttaata tcatcgctat ggctgtaacc gatattggca ttgccgtaac
    14341 caccgcgata attcagcgtg gcgtagcctg tacttccgct attaccatcg cctcccccgg
    14401 catagccggt ttgcacgcta taactgaggt tgttgtcttc cagcaacgta ccgtatacac
    14461 cagccagatt ggtcatccga ccgttgagat cgtgtgacat gctgtagctg gcactggcat
    14521 gtcgccactg agatttactg tcagaacgca gccagtggct gaaaggaata ttgacgttaa
    14581 gcgctaacat ctgatcccgt cctttttgcc aggcgttttt cgtcaggcta tagctgagcg
    14641 tccagttgat atcttcgaac gcagtattta atccagcctg gaattgctca tcgacattac
    14701 tcgttcccca ataagtttga tggctaccac tcaaatacag tgttgatgtg cgcccgagtt
    14761 gctgagtaac ggtgagctgt aatttcccgc gtttgttata agcgaggttg taatagtcgg
    14821 tgaatttcgg cttaacctga ataactccat cctgtgtttc gatgttgtag ccattcattc
    14881 gactgtatgt tgtatcagcg aaattaaaat atccgctggt cgaataacgg taacccacta
    14941 actgaatatt cgtgcctgat tcattgagcg atttgttata gagaaaacgc accgattgtc
    15001 cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc acagacagag
    15061 cgcccagtgc ctccatgttt ttcccgatac cgaaattaaa agcacgataa cgatccgcca
    15121 gttgcgttcc accatatatt gtccagccag ccggaaggcc gtggagtaat gtactctgga
    15181 aaaagcgggg tttttcctgc tgcgcatttc cactacggta ttctcctgcc ataatggaat
    15241 aacgagtatg cccttcacgt tgcaaaagcg ggactgacga atagggtacg gtaaaaatct
    15301 gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc accactatta cctgcggcat
    15361 agatatcgtt gatggtaaaa ggccccggtg gcaccgtact attataaatg ccatatccat
    15421 tttgtttaat agtgacctgt gcagtaccat gagcaatacc gtggatcacc ggggcaaatc
    15481 ctctttggct atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa
    15541 taccatcgaa aatatcgccc tgggtataac catcacccaa cgtcagccgg gaacgtaaag
    15601 gaattatgtc tcgctcaatc caggtaatga tatgctgcca tttatttttg ctacctgatg
    15661 aactgtcgct actgttataa ctccaggtgg tattgtcgcg taaacgccac gcaccaatat
    15721 ttaacccact ctgtaggttt aaatatgcat aatggctgtt acccccaatc cgattctgta
    15781 cactatttcc gctgaaatta taattgagca atccggcatt aataccggga tcccataact
    15841 caggaggaat ataaccacgc gcgcgattac tcataaatgc ctgagggatc gtcaggttca
    15901 gtcgctgctg accaacatcc agatgcgcag tagcgtcctg gaccattgtg gttaatggca
    15961 cacaggcatc atccgccagc agattcatac cggcgacaga agccgtattc agccccatac
    16021 tggcgagttg cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg cccgtattaa
    16081 atgtgacatc acgcgttgcc atataaccat tattcaaata gatatcgacg cgatacgtcc
    16141 ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc ggccacagcc tggggatcat
    16201 ccgctaaaaa gcgcagatta aaatagaggt cggcagatga caaaggtgcc tgtgcggcaa
    16261 aagcacaggc gacaacgagt cggacaaaaa aaccagccaa acgatgctta cgaatatgca
    16321 agcattgtgt gtttcgctgg taaagtctta aattcagata tgacattatt cctccggttt
    16381 aaaatcaccc ggcagtcaat tctttttatt caggcgaaaa attccccctg cgtt
//
LOCUS       DAJTYI010000085        16356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYI010000085 DAJTYI010000000
VERSION     DAJTYI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..1984
                     /gene="pbpC"
                     /locus_tag="OO564_002658"
                     /pseudo
     CDS             1..1984
                     /gene="pbpC"
                     /locus_tag="OO564_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2134..2565
                     /gene="ndk"
                     /locus_tag="OO564_002659"
     CDS             2134..2565
                     /gene="ndk"
                     /locus_tag="OO564_002659"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6653733.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2715..3869
                     /gene="trmG/rlmN"
                     /locus_tag="OO564_002660"
     CDS             2715..3869
                     /gene="trmG/rlmN"
                     /locus_tag="OO564_002660"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6653734.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4154..5167
                     /gene="rodZ"
                     /locus_tag="OO564_002661"
     CDS             4154..5167
                     /gene="rodZ"
                     /locus_tag="OO564_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6653735.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5194..6312
                     /gene="ispG"
                     /locus_tag="OO564_002662"
                     /gene_synonym="gcpE"
     CDS             5194..6312
                     /gene="ispG"
                     /locus_tag="OO564_002662"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6653736.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6423..7697
                     /gene="hisS"
                     /locus_tag="OO564_002663"
     CDS             6423..7697
                     /gene="hisS"
                     /locus_tag="OO564_002663"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6653737.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7715..8335
                     /gene="yfgM"
                     /locus_tag="OO564_002664"
     CDS             7715..8335
                     /gene="yfgM"
                     /locus_tag="OO564_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6653738.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8346..9524
                     /gene="bamB"
                     /locus_tag="OO564_002665"
     CDS             8346..9524
                     /gene="bamB"
                     /locus_tag="OO564_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6653739.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9642..11114
                     /gene="der"
                     /locus_tag="OO564_002666"
     CDS             9642..11114
                     /gene="der"
                     /locus_tag="OO564_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6653740.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11184..11399
                     /locus_tag="OO564_002667"
     CDS             11184..11399
                     /locus_tag="OO564_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6653741.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11396..12766)
                     /gene="xseA"
                     /locus_tag="OO564_002668"
     CDS             complement(11396..12766)
                     /gene="xseA"
                     /locus_tag="OO564_002668"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6653742.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12928..14394
                     /gene="guaB"
                     /locus_tag="OO564_002669"
     CDS             12928..14394
                     /gene="guaB"
                     /locus_tag="OO564_002669"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6653743.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14463..16040
                     /gene="guaA"
                     /locus_tag="OO564_002670"
     CDS             14463..16040
                     /gene="guaA"
                     /locus_tag="OO564_002670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6653744.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16135..>16356)
                     /locus_tag="OO564_002671"
     CDS             complement(16135..>16356)
                     /locus_tag="OO564_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6653745.1"
                     /translation="SLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEAD
                     ANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3803 a         4079 c         4587 g         3887 t
ORIGIN      
        1 gacgggttat ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc
       61 accccaaaac atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc
      121 atttgtctaa gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcgttacgt
      181 tgcaggggat cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct
      241 attccgaggc ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc
      301 gttggccgga gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag
      361 gtgtgtggtc ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc
      421 gacaaatgcc gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg
      481 acaaaatcgt tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa
      541 ctggaaaggg cggttgccac cgcgcagctc actggcgatg atcgtggtta atcataccga
      601 tatgcgtgtt cgcggctggg tgggatcggt tgatctcaac gatgattcac gctttggtca
      661 tgttgatatg gtcaatgcga tccgatcgcc aggatcggtg ctcaaaccgt ttgtttatgg
      721 tctggcgctg gatgaaggct tgatccaccc ggcatcactg ctgcaagacg tcccccggcg
      781 caccggtgat tatcgaccag gtaactttga tagcggtttt catggcccga tcaacatgag
      841 cgaggcgctg gtgcgctcgc tgaacttacc tgctgtgcag gtgctggaag cctatggacc
      901 gaaacggttt gcggcaaagt tacgcaatgt tggattgccg ttatatttgc ccaacggcgc
      961 tgcgccgaat ctttcactca ttctcggcgg cgctggtgca aaactggaag atatggcggc
     1021 agcgtatacc gcgtttgctc gccacggcaa ggcaggcaaa ttgcgcttac agcctgacga
     1081 tccgctgctt gaacggcctt taatgtcgtc gggggcggcg tggatcattc gccggattat
     1141 ggctgatgaa gcgcaaccct tgccggatag tgccttgccg cgcgtcgccc cactggcatg
     1201 gaaaacgggc accagctatg gctatcgtga cgcctgggcg attggggtta acgctcgcta
     1261 tgtcattggg atctggactg gcagacctga cggcacgccc gttgttggtc agtttggctt
     1321 tgccagtgcc gtaccattgt tgaatcaggt caataatatc ttactgtcgc gcagtgcgaa
     1381 tctgccggaa gacccgcgcc cgaactcagt cactcgtggc gttatctgct ggccgggcgg
     1441 acagtctttg ccggaaggtg acggtaactg tcgccgccgc ctggcaacct ggctgctgga
     1501 tggcagccag ccgccaacgc tgttactgtc agagcaggaa gggattaatg gcattcgctt
     1561 ccccatctgg ctggatgaca aagggcagcg cgtcgccgct gactgcccgc aggcgaagca
     1621 agaaatggta aatgtctggc cgctaccgct ggaaccctgg ctgcccgcat cagaacgtcg
     1681 cgctgtgcgc ttaccaccgg cctcgacaat ttgcccgcca tacggtcacg atgcccagct
     1741 ccctctgcag ctgacaggcg ttcgcgatgg cgcgattatt aaacgtttac cgggcgcagc
     1801 ggaagcgact ttgccgttgc aatcaagtgg aggggcaggt gaacgctggt ggtttctgaa
     1861 tggcgaaccg ttaactgaac gcgggcgcaa cgtgaccctg catttgacgg ataaaggtga
     1921 ttatcaattg ctggtgatgg atgatgtggg gcaaatcgcg acagtgaaat ttgtcatgca
     1981 atagtctgtt ttgtccctga ttgttgctaa aactcatttt attttaaaaa aaatgttacc
     2041 ttcatcaata gtcaacggcc ctgttgctca ttataatccg cgccatctcg tacgctggta
     2101 cagacaacaa cagaacaatt tacagaggta aaaatggcta ttgaacgtac tttttccatc
     2161 atcaaaccga acgcggtagc aaaaaacgtc attggtaata tctttgcgcg ctttgaagct
     2221 gcagggttca aaattgtcgg caccaaaatg ctgcacctga ccgttgaaca ggcacgtggc
     2281 ttttatgctg aacacgatgg aaaaccgttc tttgatggtc tggttgaatt catgacctct
     2341 ggcccgatcg tggtttccgt gctggaaggt gaaaacgccg ttcagcgtca ccgcgatctg
     2401 ctgggcgcga ccaatccggc aaacgcactg gctggtactc tgcgcgctga ttacgctgac
     2461 agcctgaccg aaaacggtac ccacggttct gattccgtcg aatctgccgc tcgcgaaatc
     2521 gcttatttct tcggcgaagg cgaagtgtgc ccgcgcaccc gttaataatt tcgtaaatgc
     2581 cgcgtgcaaa cgtggcatcc gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc
     2641 agccgctcac cggggcgttt cttttttcaa ccctccaggg gccataacgt gtaataacga
     2701 ggccggaata gcatatgtct gaacaattag tcacacctga aaacgtcacc acgaaagatg
     2761 gaaaaatcaa cctgctggat ctcaaccgtc agcagatgcg ggagtttttt aaagatttag
     2821 gtgaaaaaac cttccgcgcc gatcaggtga tgaagtggat gtatcactat tgctgcgaca
     2881 actttgatga gatgaccgac atcaacaaag tgttgcgcgg caaactgaaa gaggtggcgg
     2941 aaatccgcgc accggaagtg gttgaagaac agcgttcatc cgacggcacc attaaatggg
     3001 cgatcgccgt tggcgatcag cgcgtcgaaa cggtgtatat cccggaagac gaccgtgcca
     3061 cgctctgcgt ctcttcgcag gtggggtgtg cgctggagtg taaattctgt tccaccgccc
     3121 agcagggctt taaccgcaac ctgcgggtat cggaaattat tggccaggtg tggcgtgcgg
     3181 cgaaaatcgt cggcgcagca aaagtcaccg gtcagcgccc aatcactaac gtcgtgatga
     3241 tgggcatggg cgagccgctg ctcaacctga acaacgtcgt tccggcgatg gaaatcatgc
     3301 ttgatgattt cggttttggc ctgtctaaac gtcgcgtgac gctttccact tccggtgtag
     3361 ttccggcgct ggataaactg ggcgatatga tcgacgttgc actggcaatt tccctgcacg
     3421 cgccgaacga cgaaattcgt gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa
     3481 cgttccttgc tgcggtgcgc cgttatctgg agaaatccaa cgccaatcag ggccgagtca
     3541 ctattgaata cgtgatgctt gaccacgtta acgacggcac tgaacacgcg caccaactgg
     3601 cggaactgct gaaagatacg ccgtgtaaga tcaacctgat cccgtggaac ccgttcccgg
     3661 gcgcgccgta tggacgcagt tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga
     3721 gctacggttt caccactatt gtgcgtaaaa cgcgtggtga tgatatcgat gctgcctgtg
     3781 gtcagttggc gggcgatgtt atcgaccgta cgaaacgtac tctgcgtaaa cggatgcagg
     3841 gtgaagccat cgatattaaa gcggtctgat agccattacg ccacggttac tttatgtacc
     3901 gtggcgtaat cttttatgaa tgaagtatta agttgcaatt gtctggtcaa tattaacggt
     3961 gcgtttttgc tgactttaag gcagtatgta acgatgcaac agtaagttag cctcagtgaa
     4021 tgtgggcttt gtcacgagca cacagacggt cttatactgt atgataacgg ttaacttaac
     4081 ggatgtttcg cggtgtgggt gagcatgatg gttcaccggc atctcaattc tcatttaaac
     4141 gtacctgcag cgaatgaata ctgaagccac gcacgaccaa aatgaagcac ttactaccgg
     4201 cgctcgcctg cgtaatgctc gcgaacaact aggacttagt cagcaggccg ttgccgagcg
     4261 actttgcctg aaggtttcca cggtacgcga cattgaagaa gataaggcac ccgccgatct
     4321 tgcttcaaca ttcctgcgcg gatatatccg ctcttatgcg cgtctggtac atattccaga
     4381 agaagaactg ctgccagggc tggaaaagca ggctccactt cgggctgcaa aagttgcgcc
     4441 aatgcagagt ttttccctcg gtaaacgccg caaaaaacgc gacggctggc tgatgacctt
     4501 cacctggctg gtgttgtttg tggttatcgg cctgagcggt gcctggtggt ggcaagacca
     4561 caaagctcag caggaagaga tcaccactat ggccgatcaa tcttcggcgg aactgagcag
     4621 taatagcgag caggggcaga gtgttccgtt aaatacgtcg acaactacag acccggctac
     4681 aacgtccacg ccgccagcgt ctgtggatac taccgcaacc aacacgcaaa cacctgccgt
     4741 aactgcgcca gcaccagctg ttgatccgca acagaatgcg gttgtttcgc cttcgcaggc
     4801 aaatgttgat accgccgcga ccccggtacc aacggcagca acaacgccag atggtgctgc
     4861 gccgttgcca accgatcagg ctggcgtgac cacgccggcg gctgatccga atgcgctggt
     4921 gatgaacttt actgccgatt gctggctgga ggtcactgat gctaccggta aaaaattgtt
     4981 tagcgggatg cagcgtaaag acggtaattt gaatttaacc ggccaggcac cgtataaact
     5041 gaaaattggt gcgccagccg cagtacagat ccagtatcaa gggaaacctg tcgatctgag
     5101 tcgttttatc agaactaacc aggttgcgcg tctgaccctc aatgccgaac aatcaccggc
     5161 gcagtaacag acgggtaacg cgggagattt ttcatgcata accaggctcc aattcaacgt
     5221 agaaaatcaa cacgtattta cgttgggaat gtgccgattg gcgatggtgc tcccatcgcc
     5281 gtacagtcca tgaccaatac gcgtacgaca gatgtcgaag caacggtcaa tcaaatcaag
     5341 gcgctggaac gcgttggcgc tgatatcgtc cgtgtctccg taccgacgat ggacgcggca
     5401 gaagcgttca aactcatcaa acagcgggtt aacgtgccgc tggtggctga catccacttc
     5461 gactatcgca ttgcgctgaa agtagcggaa tacggcgtcg attgtctgcg tattaaccct
     5521 ggtaatatcg gtaatgaaga gcgtattcgc atggtggttg actgtgcgcg cgataaaaac
     5581 attccgatcc gtattggcgt taacgccgga tcgctggaaa aagatctgca agaaaagtat
     5641 ggcgaaccga cgccgcaggc gttgctggaa tccgccatgc gtcatgttga tcatctcgat
     5701 cgcctgaact tcgatcagtt caaagtcagc gtgaaagcgt ctgacgtctt cctcgctgtt
     5761 gagtcttatc gtttgctggc aaaacagatc gatcagccgc tgcatctggg gatcaccgaa
     5821 gccggtggtg cacgcagcgg ggcagtaaaa tccgccattg gtttaggtct gctgctgtcg
     5881 gaaggcatcg gcgacacgct gcgcgtatcg ctggcggccg atccggtcga agagatcaaa
     5941 gtcggtttcg atattttgaa atcgctgcgt atccgttcgc gagggattaa cttcatcgcc
     6001 tgcccgacct gttcgcgtca ggaatttgat gttatcggta cggttaacgc gctggagcaa
     6061 cgcctggaag atatcatcac tccgatggac gtttcgatta tcggctgcgt ggtgaatggc
     6121 ccaggtgagg cgctggtttc tacactcggc gtcaccggcg gcaacaagaa aagcggcctc
     6181 tatgaagatg gcgtgcgcaa agaccgtctg gacaacaacg atatgatcga ccagctggaa
     6241 gcacgcattc gtgcgaaagc cagtcagctg gacgaagcgc gtcgaattga cgttcagcag
     6301 gttgaaaaat aataacgtga tgggaagcgc ctcgcttccc gtgtatgatt gaacccgcat
     6361 ggctcccgaa acattgaggg aagcgttgag ggttcatttt tatattcaga aagagaataa
     6421 acgtggcaaa aaacattcaa gccattcgcg gcatgaacga ttacctgcct ggcgaaacgg
     6481 ccatctggca gcgcattgaa ggcacactga aaaacgtgct cggcagctac ggttacagtg
     6541 aaatccgctt gccgattgta gagcagaccc cgctattcaa acgtgcgatt ggtgaagtca
     6601 ccgacgtggt tgaaaaagag atgtacacct ttgaggatcg caatggcgac agcctgactc
     6661 tgcgccctga agggacggcg ggctgtgtac gcgccggcat cgagcatggt cttctgtaca
     6721 atcaggaaca gcgtctgtgg tatatcgggc cgatgttccg tcacgagcgt ccgcagaaag
     6781 ggcgttatcg tcagttccat cagttgggct gcgaagtttt cggtctgcaa ggtccggata
     6841 tcgacgctga actgattatg ctcactgccc gctggtggcg tgcgctgggt atcttcgaac
     6901 acgtaactct tgagctgaat tctatcggtt cgctggaagc acgcgccaat taccgcgatg
     6961 cgctggtggc attccttgag cagcataaag aaaagctgga cgaagactgc aaacgccgca
     7021 tgtacactaa cccgctgcgc gtgctggatt caaaaaatcc ggaagtgcag gcgcttctca
     7081 acgacgctcc ggcattaggt gattatctgg acgaggaatc tcgtgagcat tttgccggtc
     7141 tgtgcaaact gctggagagc gcggggatcg cttacaccgt aaaccagcgt ctggtgcgtg
     7201 gtctggatta ctataaccgt accgttttcg agtgggtgac taacagtctc ggctcccagg
     7261 gcaccgtgtg tgcaggcggt cgttatgacg gtcttgtgga acaactgggc ggtcgtgcaa
     7321 caccggctgt cggttttgcg atgggcctcg aacgtcttgt attgttagta caggccgtta
     7381 atccggaatt taaagccgat cctgttgtcg atatatacct ggtggcttca ggtgctgata
     7441 cacaatctgc ggctatggca ttagctgagc gtctgcgtga tgaattactg ggcgtgaaat
     7501 tgatgaccaa ccacggcggc ggcaacttta agaaacagtt tgcccgtgct gataaatggg
     7561 gtgcccgcgt tgctgtggtg ctgggtgagt ctgaagtggc taacggcaca gcagtagtga
     7621 aggatttgcg ctctggtgag caaacggcag ttgcgcagga tagcgtagcc gcgcatttgc
     7681 gcacgttact gggttaagga aggagaagga cagcgtggaa atttacgaga acgaaaacga
     7741 ccaggtagaa gcggttaaac gcttttttgc tgaaaatggc aaagcactgg ctgttggggt
     7801 gattttgggc gttggcgcac tgattggctg gcgctactgg aacagccatc aggttgattc
     7861 tgcacgctcc gcttctcttg cctatcaaaa tgcggttacc gcagtgagcg aaggcaaacc
     7921 ggatagcatc ccggcggcgg aaaaatttgc tgctgaaaat aaaaatactt atggtgcgct
     7981 ggcttctttg gaacttgcgc agcaatttgt tgacaaaaat gaactagaga aagctgccgc
     8041 ccagttacaa caggggctgg cagacacgag cgatgaaaat ctcaaagccg tgataaatct
     8101 gcgtcttgct cgcgttcagg tacagctcaa gcaggctgat gccgcgctga aaacccttga
     8161 taccatcaaa ggtgaagggt gggctgccat tgttgccgac ctgcgtggtg aagcattgct
     8221 gagcaaaggt gataagcaag gtgcgcgtag tgcatgggaa gcaggcgtga aaagcgatgt
     8281 tactccggca ctgagcgaaa tgatgcagat gaaaattaat aatttgtcca tctgagaggg
     8341 acccgatgca attgcgtaaa ttactgctgc caggactgct ttccgttacc cttttaagcg
     8401 gctgttcgct gtttaacagc gaagaagatg tggtaaagat gtccccattg ccaaccgttg
     8461 aaaaccagtt tacgccgacc acggcgtgga gcacttccgt tggtagcggc attggcaact
     8521 tctattccaa tcttcatccg gcactggcgg acaacgttgt ctatgcagcg gaccgcgctg
     8581 gtttggtaaa agcgctgaat gcggatgatg gcaaagaaat ctggtctgtc agcctggccg
     8641 agaaagatgg ctggttctct aaagagcctg cattactttc tggcggtgtg accgtgtctg
     8701 gtgggcatgt ctacattggc agcgaaaagg cgcaggttta cgcgctgaat accagcgatg
     8761 gtactgtggc atggcaaact acagtcgcgg gtgaagcact ttcgcgcccg gtggtcagcg
     8821 acggtctggt gttaatccac accagtaacg gtcagttaca agcgctgaac gaagctgacg
     8881 gcgctgtcaa atggacagtt aacctcgata tgccttcgct ctctttgcgt ggcgagtctg
     8941 cgccggcaac ggcttttggt gcggccgtcg tgggtggcga taatggtcgc gtcagcgcag
     9001 tgctgatgga acagggccag atgatttggc agcagcgtat ttcccaggcg accggttcta
     9061 ccgaaattga ccgtctgagc gatgttgaca cgactcccgt cgttgttaac ggcgttgttt
     9121 tcgcgctggc ctataatggt aacctgacgg cgcttgatct gcgcagtggt cagattatgt
     9181 ggaaacgcga actgggttcg gtgaatgatt tcatcgtcga tggcaatcgc atctatctgg
     9241 tcgatcaaaa tgaccgggtg atggcgttga ccattgatgg cggcgttacg ctgtgggcac
     9301 aaagcgatct gctgcatcgc ctgctgactt ctccggtgct gtataatggc aacctggtgg
     9361 tcggtgacag tgaaggttat ctgcactgga ttaacgtcga agatggtcgt ttcgttgccc
     9421 agcaaaaagt tgatagttcc ggtttccaga ctgaaccggt tgccgctgac ggcaaactgc
     9481 tgatccaggc aaaagacgga accgtgtact ctattacacg ttaatcgtct ctgtcgttca
     9541 ctttgaaaac ggctcctgga caggggccgt tttcctgttt ttaacaacga cgcgaatata
     9601 gcgtgcgttg tctgatgatt tataaaaatg aggctttaaa catggtacct gtggtcgcgc
     9661 ttgtcgggcg ccctaacgta ggaaaatcca cgttatttaa ccgtctaact cgcacccgag
     9721 atgcgctggt tgcggatttc ccgggtctga ctcgtgaccg taagtacggt cgtgcggaaa
     9781 ttgaaggccg tgagtttatc tgtattgata ccggcgggat tgatggcaca gaagacggtg
     9841 tagaaacccg catggcggaa cagtcgctgc tggcgattga agaagcggac gtcgtactgt
     9901 ttatggtgga tgcgcgcgcg ggcctgatgc cggcagatga agcgattgcc aaacatctgc
     9961 gctcccgtga aaaaccgacc ttcctggtgg caaacaaaac tgacggtctg gatcccgatc
    10021 aggcagtggt tgatttctac tcgcttggtt taggtgaaat ctacccgatc gccgcgtctc
    10081 acggtcgtgg cgtattaagt ctgctggagc atgtgctgct gccgtggatg gaagatctcg
    10141 caccgcaaga ggaagtcgac gaagacgctg aatactgggc gcaatttgaa gcggaagaga
    10201 acggcgaaga agaagaggaa gacgacttcg acccgcaaag tctgccgatc aaactggcga
    10261 ttgtgggtcg tccgaacgta ggtaagtcta cactcactaa ccgtattctt ggagaagagc
    10321 gcgttgttgt ttacgacatg cctggcacga cgcgtgacag catctacatc ccaatggaac
    10381 gcgatggacg tgagtatgtg ctcattgaca ccgctggcgt acgtaaacgc ggcaaaatca
    10441 ccgatgctgt agagaaattc tccgtaatca aaacgttgca ggccattgaa gacgccaacg
    10501 tggtgatgtt agtgattgat gcgcgcgaag gtatttccga tcaggatctc tcgctgctgg
    10561 gctttattct caatagtggg cgctcacttg tcattgtggt gaataagtgg gatggcctga
    10621 gccaggaagt gaaagagcag gtgaaagaaa cgctggactt ccgtctgggc tttatcgatt
    10681 ttgctcgtgt gcactttatc tctgccttgc acggcagtgg tgttggtaac ttgtttgaat
    10741 cagtacgtga agcgtatgac agctccaccc gtcgtgtggg gacctctatg ctgacgcgca
    10801 tcatgacgat ggctgttgaa gatcaccaac cgccgctggt acgcggtcgt cgtgtgaagc
    10861 tgaaatatgc ccacgctggt ggttataacc cgccgattgt ggtgattcac ggtaatcagg
    10921 tgaaagacct gcctgattcc tacaagcgct acttgatgaa ctacttccgc aaatcgctgg
    10981 acgtaatggg atcgccgatt cgtattcagt tcaaagaagg ggaaaacccg tatgcgaaca
    11041 agcgtaacac cctgacgcca acccagatgc gtaaacgtaa gcgtctgatg aagcacatca
    11101 agaaaaataa ataattatcc gtaagtattt tatctgccat tccctctatg aatgtagagg
    11161 gaatggctac cggaggaatt catatggaac tgcattgtcc aaaatgtcag catgtgcttg
    11221 atcaggataa cggccatgcg cgttgcccat cctgcgggga atttattgaa atgaaagctc
    11281 tttgtccaga ctgccatcaa ccgttgcagg tgttaaaagc ctgcggtgca gtagattact
    11341 tctgtcagca cgggcatggc ttgatatcga aaaaacgcgt tgaattcgtg ctggcttaat
    11401 gaaccttttt acgcgatttt tttaccggct ggatgttttt tacttcactt tctatccagc
    11461 cgtcttccag acgtgtggtt agcatttcac ccgctttaac ttgcttcact tttttcagta
    11521 cattgccgtc agtagcagta gtaacgctgt atccacgcgc cagcgttgac agtgggctta
    11581 cggcttcgag gtgcgttact gcattaccga aacgttcacg cgtggcgcta agctgtgcgc
    11641 gcaggatttc tgctaaacga tattccagtt gctgaatgcg cgtttgcgtg cgatgaatct
    11701 tcggctgtgg attttgctga ttcagccgct gtgttaatcg ctgctgctgt tgcccggcac
    11761 gcttaagctg attttccagc gcaaagctca tccgtttttg caggcgttca agcatggttt
    11821 gctggcgtgc cagccggagc tgcggatgct gctgctgcaa gcgatgatga atctgcgtaa
    11881 agcgacgcgt acggttggcg agataataat ccatcgccat ctccagccgt tgacgggccg
    11941 attgcacctg gcgcagtaac tcttgctgat tacggctcac tacttcagcg gcggcagacg
    12001 gcgttggcgc acgcagatcg gcaacaaaat cggcaatggt cacatccgtc tcatgcccga
    12061 cggcgctgac gaccggaatg cggctggcaa aaatcgcccg cgctacgcgt tcgtcgttaa
    12121 aactccataa atcttccagc gaaccgccgc cgcgcccaac gatcaacacg tcgcactcat
    12181 tgcgctgatt cgccagttca atggcgcgaa caatttgccc cggcgcgtca tcgccctgaa
    12241 cggaggtggg gtagatgatc accggtagag aaggatcgcg acgttttaac acatgcaaaa
    12301 tatcatgtag cgcagcaccg gtttttgagg tgatcacacc aacgcaatgc gcaggggagg
    12361 gaagtggttt tttgtattgc tgatcgaaca aaccttcagc ctgcaacttc gctttgagct
    12421 gttcgtactt ctgttgcagc agcccttcac cggccggctg catactctca acgattatct
    12481 ggtagtcgcc gcgcggctcg tagagcgtaa tattggcgcg aactaaaact tgttgcccat
    12541 gctgcggacg gaaggtgacc cggcggttgc tgttgcggaa catcgcgcag cgtacctggg
    12601 cggtgtcgtc tttgagtgta aagtaccagt gaccggaagc cggttgtgtg aaattagaga
    12661 tttcgccgct gatccaaacc tgtcccatct catgctcaag cagcagacga accgtttgat
    12721 tcaggcgact aacggtaaaa attgcagggg attgagaagg taacatgtga gcgagatcaa
    12781 attctaaatc agcaggttat tcagtcgata gtaacccgcc cttcggggat agcaagcatt
    12841 ttttgcaaaa aggggtagat gcaatcggtt acgctctgta taatgccgcg gcaatattta
    12901 ttaaccactc tggtcgagat attgcccatg ctacgtatcg ctaaagaagc tctgacgttt
    12961 gacgacgttc tcctcgttcc tgctcattct accgttctgc cgaatactgc tgacctcagc
    13021 acccagctga cgaaaactat tcgtctgaat atccctatgc tttccgcagc aatggatacc
    13081 gtaacggaag cgcgcctggc tattgctctg gctcaggaag gcggtatcgg ctttatccac
    13141 aaaaacatgt ccattgaacg ccaggcagaa gaagttcgcc gtgtgaaaaa acacgaatct
    13201 ggtgtggtga ctgatccgca gactgttctg ccaaccacga cgctgcgcga agtgaaagaa
    13261 ctgaccgagc gtaacggttt tgcgggctat ccggtcgtta ccgaagaaaa cgaactggtg
    13321 ggtattatca ccggtcgtga cgtgcgtttt gttaccgacc tgaaccagcc ggttagcgtt
    13381 tacatgacgc cgaaagagcg tctggtcacc gtgcgtgaag gtgaagcccg tgaagtggtg
    13441 ctggcaaaaa tgcacgaaaa acgcgttgaa aaagcgctgg tggttgatga cgaattccac
    13501 ctgatcggca tgatcaccgt gaaagacttc cagaaagcgg aacgtaaacc gaacgcctgt
    13561 aaagacgagc aaggccgtct gcgtgttggt gctgcagttg gcgcaggtgc gggtaacgaa
    13621 gagcgtgttg acgcgctggt tgccgctggc gttgacgttc tgctgatcga ctcctcccac
    13681 ggtcactcag aaggcgtttt gcagcgtatc cgtgaaaccc gtgctaaata tccggatctg
    13741 caaatcatcg gcggcaacgt ggcaacagct gcaggtgcac gcgctttggc agaagctggt
    13801 tgcagcgcgg ttaaagtcgg tatcggccct ggttctatct gtacgactcg tatcgttact
    13861 ggcgtaggtg ttccgcagat cactgccgtt gctgacgcag ttgaagccct ggaaggcacc
    13921 ggtattccgg ttatcgcaga tggtggtatt cgcttctccg gcgacatcgc caaagctatc
    13981 gccgctggcg caagcgcggt gatggtaggt tccatgctgg cgggtactga agaatctccg
    14041 ggtgaaatcg aactctacca gggccgttct tataaatctt accgtggtat gggttccctg
    14101 ggcgcgatgt ccaaaggttc ctctgaccgt tatttccaga gcgataacgc tgccgacaaa
    14161 ctggtgccgg aaggtatcga aggtcgcgta gcttataaag gtcgcctgaa agagatcatt
    14221 caccagcaga tgggcggcct gcgctcctgt atgggtctga ccggctgtgg tactatcgac
    14281 gaactgcgta ctaaagcgga gtttgtacgt atcagcggtg cgggcattca ggaaagccac
    14341 gttcacgacg tgaccattac taaagagtcc ccgaactacc gtctgggctc ctgattctct
    14401 tcgcccgact tcatgtcggg cgatttatat tatctgtttc acttgcctcg gaataagcgt
    14461 caatgacgga aaacattcat aagcatcgca tcctcattct ggacttcggt tctcagtaca
    14521 ctcaactggt tgcgcgccgc gtgcgtgagc tgggtgttta ctgcgaactg tgggcgtggg
    14581 atgtgacaga agcacaaatt cgtgacttca atccaagcgg cattattctt tccggcggcc
    14641 cggaaagtac tactgaagaa aacagtccgc gtgcgccgca gtatgtcttt gaagcaggcg
    14701 taccgatatt cggcgtttgc tatggcatgc agaccatggc aatgcagttg ggcggtcacg
    14761 ttgaagcctc taacgaacgt gaatttggct acgcgcaggt agaagtcgta aacgacagcg
    14821 cactggttcg cggtatcgaa gatgcgctga ccgcagacgg taaaccgctg ctcgatgtct
    14881 ggatgagcca cggcgataaa gttaccgcta tcccgtccga cttcgtcacc gtagccagca
    14941 ccgaaagctg cccgtttgcc attatggcta acgaagaaaa acgcttctat ggcgtacagt
    15001 tccacccgga agtgactcac acccgccagg gtatgcgcat gctggagcgt tttgtgcgtg
    15061 atatctgcca gtgtgaagcc ctgtggacgc cagcgaaaat tatcgacgat gctgtagctc
    15121 gcatccgcga gcaggtaggc gacgataaag tcatcctcgg cctctctggt ggtgtggatt
    15181 cctccgtaac cgcaatgctg ctgcaccgtg ctatcggtaa aaacctgact tgcgtattcg
    15241 tcgacaacgg cctgctgcgt ctcaacgaag cagagcaggt tctggatatg tttggcgatc
    15301 actttggtct taacattgtt cacgtaccgg cagaagatcg cttcctgtca gcgctggctg
    15361 gcgaaaacga tccggaagca aaacgtaaaa tcatcgggcg cgttttcgtt gaagtattcg
    15421 atgaagaagc gcagaaactg gaagacgtga agtggctggc gcagggcacc atctaccctg
    15481 acgttatcga atctgcggct tctgcaaccg gtaaagcaca cgtcatcaaa tctcaccaca
    15541 acgtgggcgg cctgccgaaa gagatgaaga tgggcctggt tgaaccgctg aaagagctgt
    15601 tcaaagacga agtgcgtaag attggtctgg agctgggcct gccgtacgac atgctgtacc
    15661 gtcatccgtt cccgggacca ggccttggcg ttcgtgttct gggtgaagtg aagaaagagt
    15721 actgtgacct gctgcgccgt gctgacgcca tcttcattga agaactgcgt aaagcggacc
    15781 tgtacgacaa agtcagccag gcgttcaccg tcttcctgcc agtacgttcc gttggcgtaa
    15841 tgggcgatgg tcgtaagtat gactgggttg tctctctgcg tgctgtcgaa accatcgact
    15901 ttatgaccgc acactgggcg catctgccgt acgatttcct cggtcgcgtt tccaaccgca
    15961 ttatcaatga agtgaacggt atttcccgcg tggtgtatga catcagcggc aagccgccag
    16021 ctaccattga gtgggaatga ttttgatccc tgcactatga atgaacaaaa ccctccgtta
    16081 ctacagaggg ttttttatct tcaagaatca taggcttgaa gttactaaca tcgattaatt
    16141 aaaccagctg tccgatttgt tctcttctgc tttgcccacg cttttcatca gatcgcgacc
    16201 gccttcagtc atatttctgt ttgcgtcagc ttcagattgc accacatcgg tttgcgcagc
    16261 tttgtgcttc agttcctgat cgataaattc gttttcgcgc ttaacgcggg cttcttcttt
    16321 cgccagcgcc agtttttgtt tctgaatctc taagct
//
LOCUS       DAJTYI010000086        16355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYI010000086 DAJTYI010000000
VERSION     DAJTYI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..902
                     /locus_tag="OO564_002672"
     CDS             <1..902
                     /locus_tag="OO564_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR6653746.1"
                     /translation="RSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQTQGQL
                     AKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEASVASAQLQ
                     LDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTLPESDIATVVQ
                     AQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKARFNNQDDALFPNQF
                     VNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVTPGIQDSQKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            902..4051
                     /gene="mdtB"
                     /locus_tag="OO564_002673"
     CDS             902..4051
                     /gene="mdtB"
                     /locus_tag="OO564_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6653747.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4052..7117
                     /gene="mdtC"
                     /locus_tag="OO564_002674"
     CDS             4052..7117
                     /gene="mdtC"
                     /locus_tag="OO564_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6653748.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7118..8533
                     /locus_tag="OO564_002675"
     CDS             7118..8533
                     /locus_tag="OO564_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653749.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8530..9933
                     /gene="baeS"
                     /locus_tag="OO564_002676"
     CDS             8530..9933
                     /gene="baeS"
                     /locus_tag="OO564_002676"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6653750.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9930..10652
                     /gene="baeR"
                     /locus_tag="OO564_002677"
     CDS             9930..10652
                     /gene="baeR"
                     /locus_tag="OO564_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6653751.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10843..11175
                     /locus_tag="OO564_002678"
     CDS             10843..11175
                     /locus_tag="OO564_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6653752.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11322..12683
                     /gene="yegQ"
                     /locus_tag="OO564_002679"
     CDS             11322..12683
                     /gene="yegQ"
                     /locus_tag="OO564_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6653753.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13025..13342)
                     /locus_tag="OO564_002680"
     CDS             complement(13025..13342)
                     /locus_tag="OO564_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653754.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13748..14647
                     /gene="yegS"
                     /locus_tag="OO564_002681"
     CDS             13748..14647
                     /gene="yegS"
                     /locus_tag="OO564_002681"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6653755.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14729..15502)
                     /locus_tag="OO564_002682"
     CDS             complement(14729..15502)
                     /locus_tag="OO564_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6653756.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15608..>16355)
                     /locus_tag="OO564_002683"
     CDS             complement(15608..>16355)
                     /locus_tag="OO564_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6653757.1"
                     /translation="FYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAF
                     IEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLAL
                     AKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLAL
                     VGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPL
                     IAHRGSFESFTQAVRDIARNAMPGKVLLIP"
BASE COUNT         3754 a         4379 c         4320 g         3902 t
ORIGIN      
        1 tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc cagcaggtca
       61 aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca ttagcacaaa
      121 cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt gatctggcgc
      181 gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat gcccaacagg
      241 cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc gccagcgcgc
      301 agttgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc
      361 aagttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg gtgatcaccc
      421 agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct actgtagtgc
      481 aggcgcaaaa agccggaaaa ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga
      541 aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca accggtacga
      601 ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat cagtttgtta
      661 acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca gccgccctgc
      721 aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac
      781 atctggtgac gccgggcatt caggacagtc agaaagtgga agtggtggaa gcccagagcg
      841 ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct
      901 gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc
      961 tgtcgccacc acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc
     1021 cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta
     1081 cccaggtgcc agcccggatg tcatgacctc tgccgttacc gcgccgctcg aacgccagtt
     1141 cgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat
     1201 cactttgcag ttccagctaa cattaccgct caatgtcgcc gagcaggaag tgcaggccgc
     1261 gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa
     1321 agtgaacccg gcagatccgc cgatcatgac gctcgccgtc acctcaaccg ccatgccgat
     1381 gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg
     1441 cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca aacttaacgc
     1501 tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc
     1561 taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgtgcggtca cgctttccgc
     1621 gaacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct accagaacgg
     1681 cgcgccaatt cgtctgggcg atgtagcaac tgtagagcaa ggtgcagaaa acagctggct
     1741 cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc
     1801 taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca ctgagagtct
     1861 gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc accaatatcc gcgcatccgt
     1921 cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct
     1981 gtttttgcgc aatattccgg cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat
     2041 cggcactttc gcggttatgg tgtttctcga tttttcaatc aataacctga cactgatggc
     2101 gttaactatc gccaccggat tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc
     2161 ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat
     2221 cggctttacc attatctcgc tgaccttctc actgattgcg gtgttgatcc cactgctgtt
     2281 tatgggcgat atcgtcgggc gactgttccg cgaatttgct attaccctgg cggtagcgat
     2341 tttgatctca gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag
     2401 ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag
     2461 gataatcgcc gcctatggtc gtggactggc gaaagtgctg aatcatccgt ggctgacctt
     2521 aagcgtggca ctcagcacgc tgctgcttag cgtggcactc agcacgctgc tgcttagcgt
     2581 gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca
     2641 gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca
     2701 ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt
     2761 tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgttgga
     2821 tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt
     2881 gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata ctcaggtcag
     2941 ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca gtacctgggt
     3001 gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg
     3061 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
     3121 gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc
     3181 cactatttat actcaggcta accagtatcg cgtggtgctg gagcacaata ccgaaaatac
     3241 cccaggcctc gcggcgctgg ataccattcg cctgaccagc agcgacggtg gcgtggtgcc
     3301 gttaagctca attgccaaaa ttgagcagcg ttttgcgccg ctttccatca accatctgga
     3361 tcagttcccg gtaacaacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc
     3421 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
     3481 attccaaggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
     3541 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
     3601 gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg cattaatgat
     3661 tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtattgt
     3721 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
     3781 gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
     3841 caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
     3901 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
     3961 gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggtattgt ggaccaaaag
     4021 tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
     4081 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggtttccg
     4141 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
     4201 gctgcccggt gcgtcgccgg agacaatggc gtcttccgtt gccacgccgc tggagcgctc
     4261 acttgggcgc attgccgggg ttagtgaaat gacctccagc agttcgctcg gcagcacgcg
     4321 cattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc
     4381 ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgcc cgacctatcg
     4441 caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
     4501 gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga
     4561 cggtgtcggt gatgtcgatg tcggtggcag ctcgctgccc gccgtgcgcg tcgggctgaa
     4621 cccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcactg ccatcagcaa
     4681 tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca
     4741 gaccaacgat gaactgaaaa ccgccgctga atatcagccg ctgattattc actacaacaa
     4801 cggcggcgcg gttcgtctgg gcgatgtggc gacggtgact gactcagtgc aggatgtgcg
     4861 caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca aactgccgga
     4921 agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccagagt tgcaggagac
     4981 cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc
     5041 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtctt
     5101 tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc cggtttcgct
     5161 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataatc tttcgctgat
     5221 ggcgctcacc attgctactg gtttcgtggt ggatgacgcc attgtggtgc tggaaaacat
     5281 tgcccgtcat ctggaagcgg ggatgaaacc actacaggcc gcgctgcaag gtactcgcga
     5341 agtcggcttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
     5401 gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc
     5461 cattggtatt tcgttgctgg tttccctgac gctaacgcca atgatgtgcg gctggatgct
     5521 aaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca tgttggtagc
     5581 cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc gtctggtggg
     5641 cgtggtgctg cttggcacca ttgcgctgaa tatctcgatc ccgaaaacct tcttcccgga
     5701 gcaggacacc ggcgtactga tgggtgggat acaggcggat cagagtattt cgtttcaggc
     5761 gatgcgcggc aaattgcagg atttcatgaa aattatccgt gacgatccgg cagtggataa
     5821 tgtcaccggc tttaccggcg gttcacgagt taacagtggg atgatgttta tcaccctcaa
     5881 gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc gcgtgaagct
     5941 ggcgaaagaa ccgggggcga atctgttcct gatggcggta caagatattc gcgttggtgg
     6001 gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg cggcactgcg
     6061 cgaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa
     6121 ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg acaccatggc
     6181 acggctggga atcgacgtac aggccgccaa cagtctgtta aataacgcct tcggtcagcg
     6241 gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg aagtggatcc
     6301 gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa
     6361 agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa
     6421 tcatcaggga ttatcggcgg cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct
     6481 ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt
     6541 gcgcggcagt tttgccggca cggcgcaggt tttccaggag acgatgaact cgcaggtgat
     6601 cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt acgagagtta
     6661 cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag cgctgttggc
     6721 gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca tgctattaat
     6781 cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag cccaacggca
     6841 cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat
     6901 tatgatgacc accctggcgg cgctgtttgg cgcgctgccg ctggtattgt cgggcggtga
     6961 cggctcggag ctgcggcaac ccctgaggat caccattgtc ggcggtctgg tgatgagcca
     7021 gctcctgacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg
     7081 tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttc ccgacagcac
     7141 ccgctggcaa ttatggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat
     7201 cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca
     7261 catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct
     7321 ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg
     7381 ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac gcgcgttaca
     7441 gggcgttggc ggtgcgatga tggtgccggt cggcaggttg acggtgatga aaatcgtacc
     7501 gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg tcggtccgct
     7561 gctcggtccg gcgctcggcg gtctgctggt agaatacgcg tcgtggcact ggatcttttt
     7621 gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta
     7681 caccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg ttggcatggc
     7741 ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc tggcgattgc
     7801 tggcctagtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg ccagaaataa
     7861 caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc tgggcctggc
     7921 ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac cggttttcct
     7981 gcaaattggc ctcggtttct cgccgtttca tgccggactg atgatgatcc cgatggtgct
     8041 tggcagcatg ggaatgaagc gaattgtggt acaggtggtg aatcgctttg gttatcgtcg
     8101 ggtactggta gcgaccacgc tgggtctgtc gctggtcacc ctgttgttta tgactaccgc
     8161 tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc
     8221 gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca atctggcgag
     8281 cagcggaaac agcctgctgt cgatgattat gcaattgtcg atgagtatcg gcgtcaccat
     8341 cgccgggctg ttgctggggc tttttggttc gcagcatgtc agcgtcgaca gcggcaccac
     8401 acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc ttccggcgtt
     8461 catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag
     8521 gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct ggcgattttc
     8581 gccacctgca ttgtcttgct gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt
     8641 ggctttattg attacatcaa acacggcaat gaacagcgat tacagctgtt aagtgatgcg
     8701 cttggcgagc agtatgcgca gcatggtaac tggcgcttcc tgcgcaacaa cgatcgcttt
     8761 gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc cggcccgggt
     8821 atgccgccgc acggctggcg tacccagttc tgggtggtcg atcaaaacaa caaagtactg
     8881 gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct ggtcaacggt
     8941 gcggaagtgg gcgcggtgat tgcctcccct gttgagcggc tgacccgcaa tactgatatc
     9001 aatttcgata aacaacagcg gcaaaccagc tggctgatcg tcgccctggc aacgttactc
     9061 gcggcgctcg ccacctttct gctggcgcgc ggtttgctgg caccggtaaa acgacttatc
     9121 gatggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa
     9181 gatgaactgg gcaaactggc gcaagacttc aaccagctcg ccagcacgct ggagaaaaac
     9241 cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac gccattagcg
     9301 gtgctgcgcg gtgaactgga agccattcag gatggcgtgc gcaaattcac gccggagacg
     9361 gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga tcttcatcag
     9421 ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga tttgatccca
     9481 ctgctggaag tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg
     9541 caattttccc tgccagacag tatcaccgta tttggcgatc gcgaccgttt aatgcagtta
     9601 ttcaataact tactggaaaa cagcctgcgc tacactgaca gcggcggcag cctgaaaatc
     9661 tctgccgagc agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc
     9721 agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac
     9781 cgcgccagcg gcggttccgg gctggggctg gcgatttgcc tgaacattgt tgaagcccat
     9841 aatggccgca ttattgccgc ccattcgcct tttggcgggg taagcattac agtagagtta
     9901 ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac gaaaacacac
     9961 cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt gattatctgc
    10021 gtgctgcgag ctatgcgccg acgcttatca gccacggcga tcaggtactg gcgtatgtgc
    10081 gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc gatggcctga
    10141 cattatgccg ggaaattcgt cgtttttccg atgttccgat cgtgatggtg acggcaaaaa
    10201 tcgaagagat cgaccgcctg ctggggctgg agattggcgc cgatgactac atctgcaagc
    10261 cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat tttgcgccgt tgtaaaccgc
    10321 aacgcgaact tcagcaacag gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc
    10381 aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt ctgcttaaaa
    10441 cgctctctca cgaaccagga aaagtgttct cccgcgagca attgctcaat catctttatg
    10501 acgactaccg cgtagtaacc gaccgcacca ttgacagcca cattaaaaat ctgcgccgca
    10561 agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc gttggttacc
    10621 gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acattacttt gttagccgga
    10681 gatggcgttc agacccgatc cggcaacatt attccacgca tggtcagcaa actgacatta
    10741 tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcagtaa acagcacctt
    10801 tagatgctgt tttcgataag caatttcaat caaggagtca ttatggctgg ttggtttgaa
    10861 ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa tggtgagact
    10921 atcctcacca gcgagctgta tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg
    10981 cgtagcaaca gcccgcaaga agagcgctat gagaaaaaaa cggcaagcaa cggcaaattc
    11041 tatttcaatc tgaaagccgc taatcatcaa attatcggct ccagccagat gtacgccacc
    11101 gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag ccagacggtg
    11161 aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggcg ctttaccaga
    11221 tcccccctac agcgctacaa tgcccgccct taaagtgggg gcctccccta accgcttcat
    11281 caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa ccggaactcc
    11341 tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc gcagatgctg
    11401 tttatgcggg ccagccgcgt tactccctac gtgtgcgcaa caacgaattc aaccacgaaa
    11461 atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat gtcgtggtca
    11521 acattgcacc acacaacgcc aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg
    11581 aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg gtgcgtgagc
    11641 acttcccaga aatgccgatc cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg
    11701 tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg
    11761 aagagattga agagatccat aatcaggtgc cggatatgga gatcgagatc ttcgttcacg
    11821 gcgcactgtg catggcctac tccggtcgct gcctgctctc tggctatatc aacaagcgcg
    11881 acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc caggaaggga
    11941 aagaagatga cgttggcaac atcgtacaca agtacgaacc gattccggtg caaaatgttg
    12001 agccgacgct gggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc
    12061 gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc atgaactcga
    12121 aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg cattcgctga
    12181 aaatcgaagg tcgtaccaaa tctttctact attgcgcacg caccgcacag gtttaccgca
    12241 aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg gaaactctgg
    12301 aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact cacgacgatt
    12361 atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt ggtgagttta
    12421 ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg
    12481 acagtcttga gctgatgacg ccgcaaggca acattaactt tacccttgag cacatggaaa
    12541 acgccaaagg cgaagctatg ccggtcgcac caggcgatgg ttatactgtg tggctcccgg
    12601 tcccgcagga tcttgagcta aattacgcgc tgctaatgcg taatttctcc ggggaaacca
    12661 cgcgtaaccc ccacggtaag tgattaattt cgattatttt tcccggatgg aaaattctta
    12721 gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct gaaaaacata
    12781 atccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat ctcccttaca
    12841 cgggcttatt ttttgtacat aagctgtaaa gaaggattga tccctgataa ccgtcttcca
    12901 ttgaccacat cgatagaatc ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg
    12961 ctcgcccagg ttccggctac gcacagccag aacgcgcatg tttgacgctt accaaaaaat
    13021 attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct gcaccttctg
    13081 tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg gctgcatcat
    13141 tttttactat attttctgca ccagatattc tgcccctggc taaagaagct tcggcttcgg
    13201 tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga ccaaatttac
    13261 cgatacttgt ttcatcatta tgcacagcac aaccagatat aagaaaaata ctaattaatg
    13321 atatagcagc tatctttttc atctcacctt cccccattaa ataccaacga cactctctag
    13381 tgtttaaata taataatggc atgattatta taattgaata ggattataat aaatgttctg
    13441 tacaacattt cctacataag taggaattac ggacattgag gcccttcagg gtaactccat
    13501 gggggcttta atatattata ttgaagatgc cactgtttag ttgaatatta ggtatatgct
    13561 cttttttgaa atttataggt ggcagccgtt agtattcgct gtccccattg caagctcctg
    13621 gtggtaacca ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg
    13681 ttctgactcc accttttgtt ttctgctcta cactatctac agaccaatca taaaggcaca
    13741 tacgatcatg gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa
    13801 tctacccttg cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg
    13861 ggtcacctgg gagaaaggtg atgccgcacg atttgtagag gaggcccgga agttgggcgt
    13921 cgcaacggtg attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat
    13981 tcagtgtgag ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga
    14041 ttttgccacc agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc
    14101 cggtaatgcc attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat
    14161 ggcgacaggc ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc
    14221 gctgggtggc gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga
    14281 ccgttgtgaa atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat
    14341 tggtaacggg cgtcaggccg gtggcggtca acaattgtgc ccgaacgcgt taattaacga
    14401 tggcttgctg caactgcgca tttttaccgg cgatgaaatt cttccggctc tcgtatcaac
    14461 cttaaaatct gacgaagata acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat
    14521 acaagcccca cacgaaatca cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt
    14581 ccatattgaa atacttccgg cggcgttgcg ttgtcgatta ccaccagatt gtccattatt
    14641 gcgttaatca aactattctc tcttgaacca gggagaatcc gtaatgccgg atgcggtgta
    14701 aacaccttat ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct
    14761 gcaatgccaa tttatccacc tctgacacag catcatcggt aacaataatg tcaatttttt
    14821 cgattggcag tacctgatta aaaccgcgac gattaaattt cgatgaatcg agaacggcaa
    14881 tcactttatt tgcggcggtg accatcgccc cgctaatcga ataaccttca ttaaaggtcg
    14941 taataccatt aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat
    15001 cctgcaatga acgctcggca atggaaccat gcatcgagcg cgttttatgg cggaccgtgc
    15061 caccacagac gaccagagta atgtctttat tttcggaaag ggcaaacgct gccgggagac
    15121 tgttggtaat caccgtgata tttttagcgg tcattaatcc ttcggcgata agcattgtgg
    15181 tacttccgct gtcgagaata acggtcatcc cttcatggat cattttgact gccgcctgcg
    15241 ctattctgtt ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg
    15301 tctcggtttc actattaccg gacattattt tcgccgcacc gccatgaaag cgcgtaacca
    15361 cgcctttttg ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata
    15421 ctcccgccag atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat
    15481 tccttcgctc gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg
    15541 aagggggacc attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc
    15601 cgcggtttca tggaatgagc aacactttgc ccggcatggc attacgagcg atgtcacgca
    15661 ccgcctgggt gaagctttca aagcttccac ggtgagcgat taatggatcc aggcttaact
    15721 tacgttctgt cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggaat
    15781 agttcatcca actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgccg
    15841 atgttaaatg cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg
    15901 caatctctac cgccagttcg acagtttgcg gtacgccagc cgtctcgagg ataagctgat
    15961 taaagcgcag ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg ctactgttaa
    16021 atgtttgcat cgcaccgaaa gattttgcca gtgccagttt ttctgagcta atgtcgatcg
    16081 ccgtcacact ctttgctccc agcgcgacag cgcactgaat agccagcagg ccaatggttc
    16141 cggcaccaat aataataacg tttttattct cacaaccttg cgctaaatga aaagcatgca
    16201 ggccaacggt aatcggctca ataaaagccc catcctcaat aggcatatcc gtgggtagag
    16261 caaagacatt ttttcgctta acgacaatat attcagcaaa tccaccatca cgccgcgagc
    16321 caataaaatc atattttgcg cactgggaat aaaac
//
LOCUS       DAJTYI010000087        16055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYI010000087 DAJTYI010000000
VERSION     DAJTYI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(255..959)
                     /locus_tag="OO564_002684"
     CDS             complement(255..959)
                     /locus_tag="OO564_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6653758.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1096..1548)
                     /gene="moaE"
                     /locus_tag="OO564_002685"
     CDS             complement(1096..1548)
                     /gene="moaE"
                     /locus_tag="OO564_002685"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6653759.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1550..1795)
                     /gene="moaD"
                     /locus_tag="OO564_002686"
     CDS             complement(1550..1795)
                     /gene="moaD"
                     /locus_tag="OO564_002686"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6653760.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1788..2273)
                     /gene="moaC"
                     /locus_tag="OO564_002687"
     CDS             complement(1788..2273)
                     /gene="moaC"
                     /locus_tag="OO564_002687"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6653761.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2276..2788)
                     /gene="moaB"
                     /locus_tag="OO564_002688"
     CDS             complement(2276..2788)
                     /gene="moaB"
                     /locus_tag="OO564_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6653762.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2810..3799)
                     /gene="moaA"
                     /locus_tag="OO564_002689"
     CDS             complement(2810..3799)
                     /gene="moaA"
                     /locus_tag="OO564_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6653763.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3787..3931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4196..5104
                     /gene="yvcK"
                     /locus_tag="OO564_002690"
     CDS             4196..5104
                     /gene="yvcK"
                     /locus_tag="OO564_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6653764.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5296..7317)
                     /gene="uvrB"
                     /locus_tag="OO564_002691"
     CDS             complement(5296..7317)
                     /gene="uvrB"
                     /locus_tag="OO564_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6653765.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7896..8573)
                     /gene="bioD"
                     /locus_tag="OO564_002692"
     CDS             complement(7896..8573)
                     /gene="bioD"
                     /locus_tag="OO564_002692"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6653766.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8566..9321)
                     /gene="bioC"
                     /locus_tag="OO564_002693"
     CDS             complement(8566..9321)
                     /gene="bioC"
                     /locus_tag="OO564_002693"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6653767.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9308..10462)
                     /gene="bioF"
                     /locus_tag="OO564_002694"
     CDS             complement(9308..10462)
                     /gene="bioF"
                     /locus_tag="OO564_002694"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6653768.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10459..11499)
                     /gene="bioB"
                     /locus_tag="OO564_002695"
     CDS             complement(10459..11499)
                     /gene="bioB"
                     /locus_tag="OO564_002695"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6653769.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11586..12875
                     /gene="bioA"
                     /locus_tag="OO564_002696"
     CDS             11586..12875
                     /gene="bioA"
                     /locus_tag="OO564_002696"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6653770.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12853..13323
                     /locus_tag="OO564_002697"
     CDS             12853..13323
                     /locus_tag="OO564_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6653771.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13877..14878
                     /locus_tag="OO564_002698"
     CDS             13877..14878
                     /locus_tag="OO564_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6653772.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14912..>16055)
                     /locus_tag="OO564_002699"
     CDS             complement(14912..>16055)
                     /locus_tag="OO564_002699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6653773.1"
                     /translation="SYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVF
                     DNVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSAD
                     ESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCG
                     STGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKN
                     TAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGG
                     VSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNL
                     TYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3815 a         4228 c         3996 g         4016 t
ORIGIN      
        1 gcctcgtgct gaaaatctaa tttattgctg ttttgcgagt atgattccat tcttttagtt
       61 gtccttagaa aaaatattaa tattcaccat caataaattt aaaaccacgc atacaaaata
      121 cttttgtttt tatggatacg gaaaacgatt ccgctcgctc ataaggcata ttttatacct
      181 ggtacttgtt aagtgtcgtt tttagtaaga aaaaaatccc gacacatggc cgggatagtg
      241 atggtgaaaa agaattaacg gcggttgccg aagatccgta acaacatcag gaacaggttg
      301 atgaagtcca gatacaaggt taacgcgcca agaatggaat atttgcgcag gttcgacgtg
      361 tcgcgggtat caatctgctc acccatattt ttcaatttct gagtgtcata cgccgtcaat
      421 ccgacaaaga caatcacgcc gatgtaggta actgcccaca tcaatgcttc gcttttcagc
      481 cagaagttga ccagcgatgc cagcacaatg ccgattaacg ccataaacag catattgccc
      541 aagccactta aatcgcgctt cgtggtgtaa ccgtacaggc tcattgcgcc gaacatcccg
      601 gcagtaacga cgaaagtact ggcgatagaa gcagcggtat agacaatgaa tatactggaa
      661 agcgtaagac ccgtcagcgc cgaataaagc ataaagagca tcgtcgttac acctgcgctc
      721 agcttttgaa tcatcgctga taacacaata accaatgcta attgcgcgat gatcagaccg
      781 attaaaaaga cacggttagt gaacaacagc tccatcacgg ccgcggaatt agccgcatac
      841 caggcaacaa atgcggtcag caacaagcca acggtcatcc agccatagac ttgcgccata
      901 taagtttgca agccagcccg gggttgtacg attgaatcag aacgtgggaa tctgtccatg
      961 acgatctcct gaagatataa ggaatatctt aaggatactg caaaatgatg aggctgtgca
     1021 tcgacgcagc gtaaacgcat gtactgagcg gtgaaattgc cggacgcagc ggtgccttat
     1081 ccggctaaca aaaaactacc agcgttttgc cgcctgcaga tcgctctccc gggcttcaac
     1141 ccagcggtcg ccttccggcg tggcttcgcg cttccagaac ggtgcgcggg ttttgagata
     1201 atccataata aactgcccgg cttcaaacgc actgctgcga tgcgcactgg tgacaccgac
     1261 aaaaacgatt tcatcgcccg gccataattc cccgatgcgg tgaatcaccg tgacgcgccc
     1321 cagcggccag cggttacgcg cttcatcaac aatttctgcc agtgcttttt cagtcatccc
     1381 cggatagtgt tcgagggtta atgctttgac gctgtcgccc aggttatggt tgcgcacctt
     1441 tccggtgaag gtgactaccg caccgtcttc gtcacgctcc gccagccacg ggtactcttc
     1501 tcctacgctg aacggctgcg ggccgacaac aattttggtt tctgccatct taacctccgg
     1561 ttaccggcgg gaggaaagct acttcgtcgc cgtcagtcag cggatggtca aaactcacca
     1621 gcgtctggtt gacggcagcc agcaatttgc catcttccag cgccagcgcc cagcgatcgc
     1681 tctgcgcagc caggtgctgg cgtaacgctt caacggttgg gtaatccgca gccacttcgg
     1741 ttgcatctgt tcccaccaac tcgcgcacct gggcgaaaaa aagaacttta atcatccgct
     1801 tccaccttaa agtcacccga cttgccgccg ctcttcgcca gcaaacgtac cggaccaatc
     1861 accatatctt tttgcaccgc tttgcacatg tcataaatgg tcagcgccgc cacggaggcc
     1921 gcggttaacg cttccatttc gacaccggtt ttcccggtca ggcggcataa ggtttctata
     1981 cgcacccgat tgtgctccgg ctcggcctgt aaattgactt caactttgct gagcatcagc
     2041 gggtgacaca gcgggatcag atcccaagtg cgttttgccg cctgaatacc ggcaatacgc
     2101 gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa taatcatcgc cagcgtctcg
     2161 ctgcgcatgg tgacaaaggc ttccgcccgc gcttcacgca cggtttccgc tttggcggag
     2221 acatccacca tgtgcgcttc gccagcggcg ttgatatggg tcagttgcga catacttatt
     2281 tcttcaaatg tggatggaaa ttacacggac gcgtacgggc atccagctgc ggcgcgatga
     2341 tattttccca tgcggtacgg cacgctttgg tcgaacccgg catggcgaaa atcagcgttt
     2401 tgttagcgac gcccgctacc gcacgagatt gcaacgtgga agtgccaatc tcttcaaacg
     2461 acaacatacg gaacacttca ccaaaacctt caacctcacg gtcgaacaac ggcagcagcg
     2521 cttcgggagc ctgatcacct tccgtcaggc cagtaccacc cgtaatcaat accacttgta
     2581 catcgtcgct ggcgatccac gcagatacct gagcgcgaat agcgtagcgg ttttctttca
     2641 caatggcttt atcgacaacg tgatggcccg cttcttgcgc cgaatcgcgc agatagtgac
     2701 cggaggtatc gtcttcttca ccgcgacgat tagaaaccgt aagaatagca atacgggtcg
     2761 ggataaattc agtgcttacc tgactcatct gatctctcct tttgacgttt tagccgccaa
     2821 tgtacgataa gttttgcgta ataccggtgt tgttttgatg caggaaatgg gtctgtttct
     2881 tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg ttgctgggta tcgtcttcca
     2941 gcagatcgcg caggttaacg ccgccttcac caaacaggca gagatggagt ttaccaatgg
     3001 aggaaacgcg caggcggttg caagtggcgc agaagtcttt ttcatacggc atgataaggc
     3061 caatctctcc ggcgtaatct ggatggcaaa agacttgcgc gggaccgtcg ctgcgttgac
     3121 gtaattggtg gatccagccg cgacgcagta gctcgtcacg cagaacctga ccagagatgt
     3181 gatgcttacg gaagagctcg ctgccctcgc ccgtttccat cagttcgatg aaacgcagct
     3241 ggatagggcg atgctggatc cagttcagaa aggtgtcgag ctggtgatga ttaacatcac
     3301 gcatcagcac ggtattgact ttgaccttct caaaaccggc ctcaaatgca gcatcaatcc
     3361 ctgccatgac ctggttgaat ttatcctgcc cggtaatagc gtgaaactgg cgggcgtcca
     3421 aactgtcgac actgacgtta atgccagtaa gtcccgcatc gcgccagttc gccacatcgc
     3481 gttccagacg gtaaccattg gtggtgaccg caatctggcg gatagcgtcg ttttcccgca
     3541 cagcggcgat gatatcggta aagtcgcggc gtaaagacgg ctctcctcct gtcaggcgca
     3601 ctttttcggt gcccaggctg gcgaaggcgc gcgtaacccg gcgaatttca tcgacggtaa
     3661 gaaagccttt attggtgacg ccgctcggtt tgtagccatc cggcaggcag taggtgcaac
     3721 gaaagttaca cacatcggta atcgacaggc gcaagtagta aaacttacgc gcaaatgcat
     3781 cagtcagttg tgaagccatg tacacctttc cagatacggg aggcgaagtc atttcttcct
     3841 tcgccctggt ggcaatcttt acgagcattg tcacggccaa agcaccgtat cagttgaccc
     3901 aggtgcagag gctagagtgt ttagtggtta agccgatact agcgtgtaaa tgagattttt
     3961 accatccaca ctttcgctat gcaatcatgt atatagcgtc atgatcgtgc tatttcgcta
     4021 cagaataagg gaaaatggcg gttttacttt gatatacatc atcagggagg catagcgtgc
     4081 atcgtccttt agagatagaa gaaaatagtt tgtatcctca atattggcag gttaattgct
     4141 gtttccccgc aatttgcgct actgtagcgc ggataatctg actccaggaa actatatgcg
     4201 caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc ggcggagggc atggactggg
     4261 acgcgttctc tcatcacttt cgtctttggg ttctcgttta acgggtatcg tcaccaccac
     4321 ggataatggt ggctcgacgg ggcgtattcg ccgttcagaa ggcggcattg cttggggcga
     4381 tatgcgcaac tgcctcaacc agctgataac ggaaccgagc gtcgcctccg cgatgtttga
     4441 ataccgtttt ggcggcaatg gcgaactttc cggtcataat ctcggaaact tgatgttaaa
     4501 ggcgctggat caccttagcg tgcggcctct ggaagccatc aatttaattc gtaatctgct
     4561 gaaagtggat acgcatttga ttccaatgtc agagcatcct gttgatctga tggcgattga
     4621 cgatcagggg catgaagttt acggcgaggt caatatcgac cagttaacta cgccgattca
     4681 agagttattg ttaacgccta atgtacccgc aacgcgtgag gcggttcacg ctatcaatga
     4741 agcggatctc atcattattg ggcctggcag tttttatacc agcctgatgc caattctgct
     4801 gctgaaggaa atcgcccagg cattacgccg cacgccagcg ccgatggttt atatcggcaa
     4861 tctggggcgt gagttgagtt tacctgcggc taatttgaag ctggaaagca agctggcaat
     4921 tatggagcag tatgttggta aaaaggtcat tgatgcggtc atcgtcgggc caaaagtgga
     4981 tgtctcggcg gtgaaagagc ggattgtgat ccaggaggta ctggaggcca gcgatattcc
     5041 gtatcgtcat gaccgccagt tgttacataa cgcgctggaa aaggcgttac aggctttagg
     5101 ttaacggaaa tccgaaggaa aattccggct tcctattgaa gacaaagtgc gcgttgttta
     5161 tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc gcgcaaattc aatatattgc
     5221 ggagaaaatg taggcctgat aagcgtagcg catcaggctg ttttccgttt gtcatcagtc
     5281 ttcttcgcta tcctgttacg atgcggcaat aaacaactca cgcaactgat gcaactggtc
     5341 acgaatttgt gccgcttctt cgaactccag attctgcgcg tgttgcatca tcagcccttc
     5401 cagttcatgg attttctgct gcaacgcttt cggcgacata tccatcggca cattatccgg
     5461 ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa tgttctgccc
     5521 cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc
     5581 gttgtacttc tgctgtttct cacggcgacg ttcggtttcg ccaatcgctt tcgccattga
     5641 tggagtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg ccgcacgacc
     5701 aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag cgtcgaggat
     5761 cgccaccagc gacacttccg gcatatccag accttcacgc agtaagttga tccctaccaa
     5821 tacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgctcga cggtgtcgat
     5881 atctgagtga agataacgca cgcgctcacc gtgttcttcg agatattcgg taagatcttc
     5941 cgccattcgc ttggtcagtg tggtgaccag cacgcgttcg ttaattgccg ctcgctgacg
     6001 aatctccgaa agaagatcat caacctgtgt cgccaccggc cgcacttcga taatcgggtc
     6061 aagcaatccg gttggacgca ccacctgatc caccacatcg ccgccggatt tttccagctc
     6121 gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc
     6181 aaacttaagc ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt
     6241 ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga
     6301 ttcatcgacg accagcagcc catcggcagg caggtaatca aacagcgtcg gcggtggctc
     6361 acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac agtagcccag
     6421 ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct cttccagcag
     6481 tttgttgttt tccaacagca ctttgcgtct ggcggccagt tcttctttga tctcctccat
     6541 cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt ttcgggtaga tggtaaaacg
     6601 tggaatagtg gaaacaatct gcccggtcag cgggtcaaat aacgacaatc gttccacttc
     6661 ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga agatatctat
     6721 cacctcgcca cgaacgcgga aagtaccacg ctggaatgct tgatcattac gagcgtattg
     6781 cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata atcataccga ccgtgagatg
     6841 gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagacgc
     6901 cactacaatc acatcacgcc gctccagcat cgctttggtg gcggacaaac gcatctgctc
     6961 aatatgttcg ttaaccgagg catctttctc aatgaaggtg tcggaactcg gtacataggc
     7021 ttccggctga tagtagtcgt agtaggagac gaaatactcc accgcgtttt ccgggaagaa
     7081 ctctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt
     7141 tgggcgctga aggtcagcaa tgacattggc aatggtgaag gttttcccgg agccagtcac
     7201 gccaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc
     7261 ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag
     7321 tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg
     7381 ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg atacagcgta
     7441 aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact ccggccttac
     7501 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga tgacagtttt
     7561 atggcaagag atgcctgtac agtgactgcc attgcattta tctaaccagt taaaaaataa
     7621 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
     7681 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
     7741 ctccagcccc tatcccccgc cgcctgggca attcccacat tcgccgcatg aggcggaagt
     7801 aaaatttttt tcgtgattat tttagaattt aattggttaa attgcagtca atcgaagacg
     7861 caatctcgct cgcaatttaa ccaaatacag aatggctaca acaaggcaag gtttatgtac
     7921 tttccggttg ccgcattttc tggattttct gcaagccagg ggatctctcc cagcagcggc
     7981 gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgta
     8041 acatcgttcg ccacccaacc cgccagagtc agtccggcgt gttgtattac ctgcgcagtc
     8101 aacatcgcgt gattaataca gccgagtttc acaccaacta ccagtatcac cggcagttgt
     8161 tcctgtgtta cccaatctgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca
     8221 gcaccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt
     8281 accaatgatt ctatcggcct gccctcttgg gcgctgatga tgtgcggcga ggtgggttct
     8341 gcgaaggtgt aaggatttac cgttgcgtaa tccagctgca ggctgctgtt gtgctgtaac
     8401 gccagcgcgt cactattacg taagccttcc ggggtctttt cgctgccaga ggcgaccggt
     8461 ttataacctg ccgtccgata acctactgcc ttcgcggctt gtaaaagtgc acaactggcg
     8521 acggttttcc ccacttcggt atccgttccg gtgacaaaat aacgtttact cacgagcaat
     8581 cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgttgttgcg gccaggccag
     8641 ttgcaatcgc tgcaactgcg aacgcgttaa tattcgcggg tcgcgccctt catgaagatg
     8701 cgtagcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accatagtgt
     8761 gatgagctga atatgatgtt gataacgcac gccgttcagc gactcttcga tttcatctgg
     8821 cggtaaaaag cgattagcat gcggacgctc gtccaccgcc tgccgcgcct gatgcagttc
     8881 gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg cctccggggc gcaccacccg
     8941 atacagctcg cggagtgccg tggataaatt accgcaccac tgcaccgcga gattgctcca
     9001 tgcaagatcg aacgtcgcag tcgctaacgg cagggattcg atatctcccg ccagataatg
     9061 atctgcggca tctttatggc gggcttgcgc aagcattggc ggcgagagat ctaacgccgt
     9121 cacctgcgcg tgacgttccc gccagtggcg gctcatccag ccaggtccac aacccgcgtc
     9181 cagtacgtgg gtgtatttac gctgtggaag cattgccagt aaggcgtcag cactctggcg
     9241 ctgtagatct gcatgttgct catagtgtgc ggctgcccga ccaaatgccg ctgcaatggc
     9301 ttgtttatta accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat
     9361 gcgcagcggt tagcgttaag cgaagtcgcg cagtaccagc gggtacggtt ggcgggcgaa
     9421 tcgccgtgac ccagcagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt
     9481 tatcaccgac gatcaatggc tggatggcgc tgcatgaatc agccagcgta aacggcaaat
     9541 cctgtactcc ggcacgaaaa cgcgcaatga gtgacaccag tttttcgcgc cgtgcatccc
     9601 cctcatcacg gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca
     9661 tactggtgct gtagataagg tggcgggcga attgcagcag ataatccgcc accgtacttg
     9721 agcaaagcac cgcagcccca ctgacgccaa atcctttgcc aaaagtcact accagcaatt
     9781 ctggttttac cttttgcagc cagcagctgc cgcgcccctg ctccccgata acgcccgtgc
     9841 cgtgggcatc atcgaccatc aaccagccat tgtgctgttg cgttacctgc tggatttccg
     9901 ccagtggcgc gctatcgccg tccatgctga acacgccttc tgtcaccacc agttgctgcc
     9961 ccggacaggg ggaagcaagc agtcgcgcca gatgagcgac atcgttatga gtaaaacggc
    10021 gaagctgcga cgggcttaaa ctggcagcct ccagcaatga ggcatggcta agccggtcgg
    10081 caacaatacg gtcctctttc gccatcatcg cggcaatgac tgcctgattc gcggcaaaac
    10141 cagagataaa cagcagcgcc cgcgaatagc caagccactc ggccagttct tcttccagcg
    10201 cctgatgcac cacgctataa ccgctgacgt gaccggagcc gccgctaccg atgccaaatt
    10261 gctccgcccc ctgcttccag gcacggataa tttgcggatg atggcttaaa cctaaataat
    10321 cgttactgga aaagttcaga tactggcgat catccgccac cagccagcgt ccggctcctt
    10381 gcgccaccgg ataacggcga cgcagggcat cggcagcacg ccgcgcatcg agcgccgcgt
    10441 tgattttctc ctgccagctc ataatgctgc cgcgttgtaa tattcgtcgg tgtccggggt
    10501 catcagcgcc tgtccaagac gttgctgttg ttcgttatcc cctgccagca cggcagtttg
    10561 ctgcggattt agccccagtt tgcggaacag ttgcaggtct ttatcttctt ccggattcgg
    10621 cgtggtcagc agtttgcaac cgtagaaaat cgagtttgcg cctgccataa agcacatcgc
    10681 ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgcacgtaag aggtcggcat
    10741 catgatccgc gctacggcaa tggtgcgaat aaaatcaaag gcatcaacgt catcgttatc
    10801 ggcaagcggc gtgcctttca ccttcaccag catgttgatt ggtacgcttt ccggcggcgt
    10861 cggcaggttt gccagttgca gcaataatcc ggcgcgatct tttaccgttt cgcctaagcc
    10921 cacaatgcca cctgagcaga ctttgatccc ggcatcgcgc actttttcca gcgtatcgag
    10981 gcgttcctga taagtgcgtg tggtgatgat attgccgtaa aactccggcg aggtgtccag
    11041 gttgtggttg tagtaatcca gcccggcgtt cgcgaggcgc tgcgcctgag attcactcaa
    11101 cgtgcccagc gtcatacacg cctccagccc catcgctttt accccctgca ccatttgttc
    11161 caggtacggc atatcgcgtt cgtggggatt cttccatgcc gcgcccatac agaagcgcgt
    11221 cgatcctgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg
    11281 ctcggcttcc agcccggttt tgtagcgcga gctttgcggg cagtatttgc aatcttccgg
    11341 acaagctccg gtcttaatcg acagcaacgt gctgacctgc acctgacgag gatcgaaatg
    11401 ctggcgatgc acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc
    11461 tgtgacttgc gacaatgtcc agcgtgggcg gtgagccatg gggcttctcc aaaacgtgtt
    11521 ttttgttgtt aattcggtgt aggcttgtaa acctaaatct tttcaatttg gtttacaagt
    11581 cgattatgac aacggacgat cttgcctttg accaacgcca tatctggcac ccatacacat
    11641 ccatgacctc ccctctgccg gtttatccgg tggcgagcgc cgaaggttgc gagctaattt
    11701 tgtctgacgg cagacgcctg gttgacggta tgtcgtcctg gtgggcggcg atccacggct
    11761 acaatcaccc gcagcttaat gcggcgatga agacgcaaat tgatgccatg tcgcatgtga
    11821 tgtttggcgg tatcacccat gcgcccgcca tcgagctatg ccgcaaactg gtggcaatga
    11881 cgccgcaacc gctggagtgc gtttttctcg cggactccgg ttccgtagcg gtggaagtgg
    11941 cgatgaaaat ggcgttgcag tactggcaag ccaaaggcga agcgcgccag cgttttctga
    12001 ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca
    12061 actcaatgca cagcctgtgg aaaggctatc tgccagaaaa cttgtttgct cccgccccgc
    12121 aaagccgcat ggatggtgaa tgggatgaac gcgatatggt tggttttgcc cgcctgatgg
    12181 cggcgcatcg tcatgaaatc gcagcggtga tcattgagcc gattgtccag ggcgcaggcg
    12241 ggatgcgcat gtaccatccg gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag
    12301 gtatcttgct gattgccgac gagatcgcca ccggatttgg tcgtaccggc aaactgtttg
    12361 cctgtgaata tgcagaaatc gcgccggaca ttttgtgcct cggtaaagcc ttaaccggcg
    12421 gcacaatgac cctttccgcc acacttacca cgcgcgaggt tgcagaaacc atcagtaacg
    12481 gcgaagccgg ctgctttatg catgggccaa cttttatggg caatccgctg gcctgcgcgg
    12541 cagcaaacgc cagcctggcg attatcgaat ccggcgaatg gcagcagcag gtggcggcta
    12601 ttgaagtgca gctgcgcgag caactggcac cagcccgtga tgccgaaatg gttgccgatg
    12661 tgcgcgtact gggggcaatc ggtgtggtcg aaaccactcg tccggtgaat atggcggcgc
    12721 tgcaaaaatt ctttgtcgaa cagggggtat ggatccggcc ttttggcaaa ctgatttacc
    12781 tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca gcggttaacc
    12841 gcgcggtaca ggatgaaaca tttttttgcc aataatggcg ataagttgcc gcatcgtcat
    12901 gtctttaacg gcatgggtta cgatggcgat aatatttcac cgcatctggc gtgggatgat
    12961 gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg acccggatgc gccaaccggc
    13021 tccggctggt ggcactgggt agttgttaat ttacccgctg atacccgcgt attaccgcaa
    13081 gggtttggct ctggtctggt agcaatgcca gacggcgttt tgcagacgcg taccgatttt
    13141 ggtaaaaccg gatacgatgg cgcagcgccg ccgaaaggcg aaacccatcg ctacattttt
    13201 accgttcacg cgctggatgt agagcgtatt gatgtcgatg aaggaggcag cggcgcgatg
    13261 gtcgggttta acgttcattt ccactctctg gcaagcgcat cgattaccgc gatgtttagt
    13321 taatcactct gccagatggc gcaatgccat ctggtatcac ttaaaggtat taaaaacaac
    13381 tttttgtctt tttaccttcc cgtttcgctc aagttagtat aaaaaagctg aacgagaaac
    13441 ataaaacgct gtaaatatca atatgttatg aaagatttag tctaaataat agactgcata
    13501 atactacaaa acacaacata tccagtcact atgaatcaac tacttagata gtattagtga
    13561 cctgaggcag agcattagcg caaggtgatt ttttgtcctc ttgcgctaat tttttgtcaa
    13621 cggactgggt tagttgaatt tacaaccatg ctaagtatct cgataagcgc agagaaatga
    13681 tgcagtggtg ggcggattgg cttgatgaga aggttaggta ggcaaatcca tgcctgagca
    13741 gagccttgcc ctctacctga gcccatgctc attctttggc ctcaacgcga gcatgacctc
    13801 actggctgga cccaaaatgg actatgacag aaagatctga tatttttcct cgcaaaaact
    13861 tagaggcttt attttaatgc ataagagcaa ctgctttgac acggcaaggc ttgtggcagc
    13921 tatgatggtt ctggtcagtc accactacgc gctctctggg cagccggagc cgtacctgtt
    13981 cggctttgaa agtgctggcg gcattgcagt tatcatattc ttttcaatat caggatatct
    14041 gatttcaaaa tcagctatca ggagtgattc ctttattgat ttcatggcta aaagagcgag
    14101 aagaatattc ccggcgctgg tcccttgctc tatactgaca tattttttat ttggatggat
    14161 acttaacgac ttcagcgcag aatattttag ccatgacatt gtcaggaaga ccataagctc
    14221 tatttttatg agtcaggctc cagatgctga cattacatca catttaattc atgccggaat
    14281 taacggcagt ttgtggaccc taccgcttga gttcctgtgt tatataataa cgggagtagc
    14341 agttgcccta ctaaagaacg gaaaagcatt catagttata ctgttggttt ttgtttctct
    14401 ttcattaatt ggaagtgtgt cagaaaacag ggatgtaatg ttctctattc cgttatggct
    14461 gtatcctttg cgtggactgg cgttcttttt tggtgccact atggcaatgt atgagaagtc
    14521 atggaatgta agtaatgtta aaattactgt agtttctttg cttgctatgt acgcctacgc
    14581 tagttatggc aaaggcattg attatactat gacttgctat atcctggttt cattctcgac
    14641 aatagccata tgcacatccg ttggcgaccc actggttaag ggcaggtttg attactcgta
    14701 tggtgtgtac atttatgcat tcccggttca gcaggttgtt ataaacacat tgcacatggg
    14761 tttttacccg tcaatgttgt taagtgccgt gactgttctt ttcttgtccc atctttcatg
    14821 gaatctggtt gaaaaaagat tcctgacacg aagttcgcct aaattatccc tggattgaac
    14881 aataaaaggg gccatatggc ccctaaaata atcagatggc cagatactca accattgaag
    14941 gcagcgcgac tcctactggg cttgggcgag taacattaat tgtcaccacg gaaacactaa
    15001 ccttgtcacc acttgatgca gggtcaccaa aaaggcttct tactctgtat gtcaggttcc
    15061 ccgggttcgg tatagctgca gatccgcttg tggcacctac cgcaactggg acattaatga
    15121 tcccatttac agacggtacg cctgaacagc cacgatacgt attgtatctg ccttccggtt
    15181 tctgcacctc ggtaatgcca gccaaataac agtcagtaac tgacacgcca ccgctacgaa
    15241 gatagcgtag aggcttattg aatatgcact tatcgaaaga aacacattca ttgatgtcga
    15301 caatatttac agcccattca tcaccagtta acgtacaacc acttacaaag acgaagatcg
    15361 ccgactgttc tatcgctata ctttgattgt ttgcagccgt gtttttgttt gcagtgttga
    15421 tatgaacacc actgacaatt ttatgtgact gcgttgaggt cggcgttcta aaatatatcc
    15481 ccgtaaattg ttcagtgcca tcggcatctg ctgctatcca tgagtttgag aaagaaaatc
    15541 ctccgtttac attctctata accacgcctg ttgaaccgca ataatctaaa tccagttgtt
    15601 ccatatgcaa atcaaggcaa tcatgaacat agacggcatt tttaaaacca atgcacatgg
    15661 tctcactgtc taaaattatt gcttctgacc taactgcctc tgacgaccac gcatacgttt
    15721 gaggctgaat cctgatgcca aagctttcat cagcagaata atttcctctg ctgaaatggc
    15781 aggagctgac actaacactt acgcactggc ctaaaaagtg aagaccgact ctacaggttc
    15841 cagcctgaca tgctataaat tttgagtgcc agagcccttg cattaaataa gcggtttcaa
    15901 cttcattcac cattacatta tcaaataccc agccataaac ctgtctgatg acagagtctt
    15961 ctgctgtaat gtttgtgtca ataccattgc aggtgttatt accagttact cgtatatttc
    16021 tcagataacg gccgatagtg tctgaatcat aagac
//
LOCUS       DAJTYI010000088        15934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYI010000088 DAJTYI010000000
VERSION     DAJTYI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1549
                     /gene="ldtD"
                     /locus_tag="OO564_002700"
     CDS             <1..1549
                     /gene="ldtD"
                     /locus_tag="OO564_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6653774.1"
                     /translation="RDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGV
                     NGMARDVVLSDAMMGYLHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQ
                     LPTFVAGLAPQHPQYAAMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQ
                     RTGMLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYD
                     NELVEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTG
                     IMVNIPAYSLLYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARK
                     DILPKVRNDPGYLESHGYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNS
                     LGRYKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDK
                     RISDALKQGDTRYVNIRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVS
                     KAEQLIR"
     gene            1730..2278
                     /locus_tag="OO564_002701"
     CDS             1730..2278
                     /locus_tag="OO564_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6653775.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2305..2952
                     /gene="gloC"
                     /locus_tag="OO564_002702"
     CDS             2305..2952
                     /gene="gloC"
                     /locus_tag="OO564_002702"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6653776.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3175..4365)
                     /gene="aspC"
                     /locus_tag="OO564_002703"
     CDS             complement(3175..4365)
                     /gene="aspC"
                     /locus_tag="OO564_002703"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6653777.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4550..5638)
                     /gene="ompF"
                     /locus_tag="OO564_002704"
     CDS             complement(4550..5638)
                     /gene="ompF"
                     /locus_tag="OO564_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6653778.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6240..7640)
                     /gene="asnS"
                     /locus_tag="OO564_002705"
     CDS             complement(6240..7640)
                     /gene="asnS"
                     /locus_tag="OO564_002705"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6653779.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7810..9012)
                     /gene="pncB"
                     /locus_tag="OO564_002706"
     CDS             complement(7810..9012)
                     /gene="pncB"
                     /locus_tag="OO564_002706"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6653780.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9278..11893
                     /gene="pepN"
                     /locus_tag="OO564_002707"
     CDS             9278..11893
                     /gene="pepN"
                     /locus_tag="OO564_002707"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6653781.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12100..12867)
                     /gene="ssuB"
                     /locus_tag="OO564_002708"
     CDS             complement(12100..12867)
                     /gene="ssuB"
                     /locus_tag="OO564_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6653782.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(12864..13511)
                     /locus_tag="OO564_002709"
     CDS             complement(12864..13511)
                     /locus_tag="OO564_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6653783.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(13523..14668)
                     /gene="ssuD"
                     /locus_tag="OO564_002710"
     CDS             complement(13523..14668)
                     /gene="ssuD"
                     /locus_tag="OO564_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6653784.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(14665..15624)
                     /gene="ssuA"
                     /locus_tag="OO564_002711"
     CDS             complement(14665..15624)
                     /gene="ssuA"
                     /locus_tag="OO564_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6653785.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(15617..>15934)
                     /locus_tag="OO564_002712"
     CDS             complement(15617..>15934)
                     /locus_tag="OO564_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6653786.1"
                     /translation="TLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSALKA
                     QEILHGVFADDSQLIDYHHRPQFTPNLQPHLDTALETFWQALHRRDVQVPDLLSLRGN
                     AHA"
BASE COUNT         3976 a         4131 c         4076 g         3751 t
ORIGIN      
        1 acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc agcaacagct
       61 ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag agttactgac
      121 cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga tgatgggcta
      181 tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata gcagtaaacc
      241 ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag cgctggataa
      301 aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat atgcggcgat
      361 gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac tgaccggcaa
      421 agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg aaatattgca
      481 acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg acacgccaac
      541 tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta agccgatgga
      601 caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct acgataatga
      661 actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag atggtgctat
      721 tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg gcgtgttggc
      781 actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga tcatggttaa
      841 cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg attcgcgagt
      901 cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta acaacgtagt
      961 ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc tgccgaaagt
     1021 gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg gctggaacag
     1081 cagagaagcg attgacccat ggcaggttga ctggtctaca atcacggcct cgaatttacc
     1141 gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata aattcaatat
     1201 gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt tcaagcgtga
     1261 tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc tggcgaatat
     1321 gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga agcagggtga
     1381 tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc tgacggcctt
     1441 tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg atctgcctgc
     1501 gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa tgaagtagtt
     1561 cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg cagcccccgt
     1621 cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt gcctcttgtg
     1681 cgccagaagt gcatataaac gataacattg acctgtagac ttgattatca tggacaaatt
     1741 cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg ccgccatcct
     1801 gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga cactcaataa
     1861 tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct atattcagga
     1921 agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa taaagtccat
     1981 cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca cgcgcaaacc
     2041 ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac gcgcccgcag
     2101 ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt tccatattga
     2161 aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag gtggtgtagg
     2221 atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc actggtagta
     2281 atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac cgcattctcc
     2341 cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt cgatcctggc
     2401 ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact gatgcagatc
     2461 ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc gcaacattac
     2521 ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg cttgcctgcg
     2581 caaagtcgta tgtttggtct ggaagagtgc cagtcgctga cgccagatcg ttggctgaac
     2641 gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg ccctgggcat
     2701 acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc tggcgatgtt
     2761 attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa tcaactgatt
     2821 tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat tccgggtcac
     2881 ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca agacgaaatg
     2941 cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga tttctggcaa
     3001 agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac aaaatcgtgc
     3061 aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc aggcaatgtt
     3121 gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta acgcttacag
     3181 cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc cggccacatt
     3241 tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca gcacttgttc
     3301 ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc tgaagtcgcg
     3361 gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct gaatacgctg
     3421 gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc tcaggatggt
     3481 ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa tcgccgcttt
     3541 catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag tacaagcgcc
     3601 aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa tcagctcttt
     3661 atgcatagcc gagaaagcgc gcagtccttc agcatcttct tccagaccac gggcaaaacc
     3721 ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt gtgccagtgt
     3781 ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc catggaacag
     3841 cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga agtcaagggt
     3901 gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag agttaaagac
     3961 gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc tggtattttt
     4021 tgccaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct gtgccgtgcg
     4081 agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct gagtgcagcg
     4141 accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat tttcgagcag
     4201 atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct catctttata
     4261 gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca gatcggccag
     4321 gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga ggttccatta
     4381 tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat tccaattatt
     4441 actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag gcataaaaaa
     4501 aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat tagaactggt
     4561 aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg tcagaatcga
     4621 tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag taggttgcgc
     4681 ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct ttcgctttag
     4741 atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc gcaactaaca
     4801 gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta gtgatcggcg
     4861 tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg tcgtacttca
     4921 gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct tcttgcaggt
     4981 tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat tcgtagctga
     5041 tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg tttttaccca
     5101 ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag ttacgatagg
     5161 tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta tcaccaccaa
     5221 attctggcag catatcggtg taacccagtg catcataaac cacaccgtag ttacggccgt
     5281 aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc gttttgttac
     5341 cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc cactgaccat
     5401 aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg gcataggtca
     5461 tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa tgcagaccaa
     5521 cagctttacc gtacagatct actttgttgc catctttgtt atagatttct gcagcgtttg
     5581 cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc ttcatcatta
     5641 tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa gttctgtcaa
     5701 taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt
     5761 ccattcaaac taacgtttcg ctaccgtgaa agtgctacaa agataaagat ttagtttcaa
     5821 aaagaaaaaa tatgtaacca aaagtaaaaa ctaaggagct ttgtgaacac cgtcatattt
     5881 ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt tacttatatt
     5941 taaattaact aaacggaaat tttgtttctg atggaaactt tatcgacctg gcacaaaatc
     6001 ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc agctaagaaa
     6061 aaactaatcc gcaataagaa tgcgggttat tttttgtgcg taaatgttgc aatgtaatat
     6121 tccgtaacag gatgatcgtt atggcggggg tgcaaataga ttgcttgact taacaaacag
     6181 ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga ggaagaaaat
     6241 cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac gttttgcacg
     6301 ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg cggaacagta
     6361 ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc catttccagc
     6421 atacgctcgt ccagaacgtc cagacgttct tcacgctggg agccaccaat gatctcaccg
     6481 atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt gaggcgcata
     6541 tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt aaagtgttct
     6601 tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac cgggttttca
     6661 aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac ctgtgcgaaa
     6721 tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg ttcagcgaag
     6781 aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt cagcatggct
     6841 tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc cagcatccag
     6901 aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg gccgaaggta
     6961 taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga tacggtcagg
     7021 aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc gttacgcggc
     7081 aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc ggtatcagat
     7141 gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa gcgatgcagc
     7201 gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt gcgcggacgc
     7261 aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat cgggtaagtg
     7321 tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc aaattgttgc
     7381 ccctggcccg gcgatgccac gactttaccc gtcacaatga ccgagcagcc ggtggtcaga
     7441 cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg tacaggatca
     7501 aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc tcggcgggta
     7561 cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg gagtacgtcg
     7621 gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa taaacacttg
     7681 tccacccgta aatggggggg gatacctatg ttacctggca tctgcaatca gacaagcaga
     7741 attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaagggagcc gattagctcc
     7801 ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc gcagcgcccg
     7861 aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt tcgccaccgg
     7921 tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct gggggatatc
     7981 gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga agtggcggta
     8041 tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt tactctgtgg
     8101 atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa ccgggtcgcc
     8161 agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat cacgcaggaa
     8221 agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat actcttccag
     8281 ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt gctgatgtgc
     8341 ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac gcgccagatc
     8401 gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga tggtttcttg
     8461 tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc gcgacatatc
     8521 aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt cgagggcttg
     8581 cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa ccgccagcaa
     8641 aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat ccagcttgcc
     8701 attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta accagttaag
     8761 atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat cctgcaggcg
     8821 caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac ccagcagatc
     8881 gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat gaaacacggc
     8941 ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa caggagaagc
     9001 gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta caataaacat
     9061 cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga tcacaccata
     9121 tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta gcaataaagg
     9181 cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca ggaatagact
     9241 gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc cacaagccaa
     9301 ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact tgacctttga
     9361 cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc atggtgcatc
     9421 agatgctccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc atattaatga
     9481 tgagccgtgg accgcctgga aagaagaaga agagggcgca ctggtcatca gtaatttgcc
     9541 ggagcgtttt acgcttaaga tcattaatga aataagcccg gcggcgaata cggcgctgga
     9601 agggctttat cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca
     9661 tattacgtat tatctcgacc gcccggacgt gctggcgcgt tttaccacca aaattattgc
     9721 cgataaaatc aaatatccct tcctgctttc caatggtaac cgcgttgcgc aaggcgaact
     9781 cgaaaacgga cgccattggg tacagtggca ggacccgttc ccgaaaccgt gctacctgtt
     9841 tgcgctggtg gcaggcgact ttgatgtact gcgcgatacc tttaccacgc gttctggtcg
     9901 cgaagtagca ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc cgtgggcgat
     9961 gacctcgctg aaaaactcca tgaaatggga tgaagaacgc tttggcctgg agtatgacct
    10021 cgacatctat atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg agaataaggg
    10081 tctgaatatc tttaactcca aatatgtgct ggcccgcacc gacaccgcca ccgacaaaga
    10141 ttacctcgat attgaacgcg ttatcggcca tgaatatttc cataactgga ccggtaaccg
    10201 agtgacctgc cgcgactggt tccagctcag cctgaaagaa ggtttaaccg tcttccgcga
    10261 tcaggaattc agctctgacc ttggttcccg cgcagttaac cgcatcaata atgtgcgcac
    10321 catgcgcgga ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa tccgcccgga
    10381 catggtcatt gagatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt
    10441 gatccgtatg atccacaccc tgctgggcga agaaaacttc cagaaaggga tgcaactcta
    10501 tttcgagcgt catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga
    10561 tgcgtcgaat gttgatctct cccatttccg ccgttggtac agccagtccg gtacgccggt
    10621 tgtgaccgtc aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca
    10681 gcgcacgcca gccacgccgg atcaggcaga aaaacagccg ctgcatattc cgtttgccat
    10741 cgaattgtat gacaacgaag gcaaagtgat cccgttgcag aaaggcggtc atccggtgaa
    10801 ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt tttgataatg tctacttcca
    10861 gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag
    10921 cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat gatttctccc gttgggatgc
    10981 ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc agcaagggca
    11041 gccgctgtct ctgccggtac atgtggctga cgctttccgc gcggtgctgc tcgatgagaa
    11101 gattgatccg gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg aaatggctga
    11161 actgttcgat atcatagacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac
    11221 tctggcgact gaactggcgg atgagctgct ggctatttac aacgcgaatt accagagcga
    11281 gtaccgtgtt gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt
    11341 ccttgctttt ggtgaaacgc atctggctga cgtgctggtg agcaagcaat accatgaagc
    11401 aaacaatatg accgatgcgc tggcagcgct ttctgcggcg gttgctgcac agctgccttg
    11461 ccgtgacgcg ctgatgcagg agtacgacga taagtggcat caggacggtc tggtgatgga
    11521 taaatggttt atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtgcgtgg
    11581 tctgttgcag catcgctcat ttaccatgag caacccgaac cgtattcgtt cgttgattgg
    11641 tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttaccagtt
    11701 cctggtggaa atgcttaccg acctcaacag ccgtaacccg caggtagctt cacgtctgat
    11761 tgaaccgctg attcgcctga aacgttatga tgccaaacgt caggagaaaa tgcgcgcggc
    11821 gctggaacag ttgaaagggc tggaaaatct ctctggcgat ctttacgaga agataactaa
    11881 agcactggct tgatactttt gagccaaaat gcctgatgcg acgcttgacg cgtcttatca
    11941 ggcctacaac tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt cacgccgcat
    12001 ccggcagccg tgcccaactg cctgatgcga tgcttgacgt gtcttatcag gcctacaagt
    12061 ccgttgaaaa tgctggtttt gtgcgccaca tccggcatat taaccatgac gccgaatcag
    12121 ttgttcagaa tgcccacgcc gcattacccg ttgcaatact tccgcttcca gctccgctaa
    12181 tcgcaccgac cctaatcgac gcggacgggg gatatccact gtcaaatcca gactaatttt
    12241 tccctcttca attaacaaca cccggtcagc cattgctacc gcttcgctca catcatgcgt
    12301 caccaacagt acggtaaagc cgtgctgctg ccaaagtgac acaatcaaat cctgcatctc
    12361 gagtcgcgtt aaggcgtcca gtgccccgag cggttcatca agcaacaata aacccggtcg
    12421 atgaatcaac gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa gtgcggcagg
    12481 ccattccccg gcgcgattct ccagtcctac cgcagccaac gcccgacgcg cggcatcgcg
    12541 ccactgacct ttaaggccta acccaacgtt atcaatcacc gatttccacg gcagcagacg
    12601 cgcatcctga aacatcattc gcgtatcatc ctgaatttca gccagcggtg tggtgcccgc
    12661 taacacatcg cctgcggttg gcgtttccag accggccagc aggcgcagca gggtactttt
    12721 accaccaccg ctgcggccca ccaccgccac aaactgacct gccggaatat gtaaatccag
    12781 ttggttcagg acgatatttt ccgcgtaatg tttgcttact gcattgagca acaatggtgt
    12841 gccctggttc agacgagcag tattcatatc gtggcctcct tcaaatgata agccgggttc
    12901 cagctcaacc agaggcgctc taacagctgc gcgctgacgt cagcaagttt gccgagcagg
    12961 gcgtaaagaa taatagcgac taccaccacg tctgtttgca aaaactctcg cgcattcatc
    13021 gccagataac caatgccgga attggcagaa atggtttcgg caacaatcag cgtcagccac
    13081 atcaggccca acgcaaaacg cacgccgacc ataattgagg gcagggcatc gggcaggatc
    13141 acatggataa acagcggtat gccggataat ccatagctac gcgccatctc caccagcccg
    13201 cgatcgatat tacggatgcc atgccaggtg ttgatataaa tggggaacag cgtaccgagc
    13261 gccaccagaa agattttcgc ggattcatca atgccaaacc acaaaatcac cagcgggatc
    13321 agcgccagat gctgccacag ttcgccgctg gcggagagcg tccagaacgc cgtcaccact
    13381 ccttccggtg aaggcaaaat acgcgtcgac agccagccaa ccgaggaggc cagttgccac
    13441 accgccacga tgcccaccgg taaaaaccag ggggcaacgc gcaataacca cttcttcact
    13501 ggcgttgcca tcatcgtgtt ccttagcttt gcgcgacttt acgggggata aaatcattcg
    13561 ccaccgcttc gccttgcgga ttcagcggct gcggctgggg aatttccgga atggcgacat
    13621 ccagatgtgg gaacagcaac tcgccaaccc gatacgcttc ttccagatgc gggtagcccg
    13681 aaagcacaaa gctgtcgatg ccaagcgcgg cgtattcgtt gattcgcgcg gcgaccgtag
    13741 gaccatcgcc caccagcgcc gttccggcac cgccgcgcac taagccaacg cccgcccata
    13801 aattggggct gatctccaga ttgtcgcgct tgccgttatg tagcgccgcc attcgctgtt
    13861 gtcctacgga atccgtccgg gcgaatgcgg cttgtgcttt ggcgatagtc tcatcatcaa
    13921 gatgcgagat taaccgctcg gcggcctgcc acgcttcgtc gttagtttca cgaacaatca
    13981 catgcagacg aataccgaaa cgaattttgc gcccatacgc ggcagctttc gcccgcactt
    14041 gttcgatttt ctctttaaca agttccggcg gttcgcccca ggtgaggtag agatcaactt
    14101 gttctgccgc cagctcctgg gcgacatctg acgatccgcc aaagtaaagc ggaggataag
    14161 gctgttgaat cggcgggaag agcagttttg ctccgcgcac atgaatatgt ttaccgttga
    14221 aattgacggt ttcccccagc aataagcgcc gccagacctg ggtaaattcc gccgaggctt
    14281 cgtagtgctc gctatgatca aggaacacgc cgtcgcctgc cagctcttgt ggatcgctgc
    14341 ctgtgaccag attaaacaac gcacgaccat ttgagagacg gtcaagcgtg gcggcctggc
    14401 gggcggcaac agtaggtgag gttacgctgg gacgcagggc gacaagaaac ttcagccgct
    14461 gcgtcaccgg gatcatcgac gctgcaacca gccacgcatc ttcacacgat cgccccgttg
    14521 ggatcagcac accggtatag cccagacgat ccgccgcttg cgcaatttgt tgcagataac
    14581 cgtgatcaac cgggcgtgaa ccttcttccg ttcccagata gtgcccgtca ccgtgggtcg
    14641 gtaaaaacca gaacatattc agactcataa ttgttttcct tccagttgag tgggctgcca
    14701 gatgcgctgg cgaatatcga cttttttcgg caccagacgg ttttcataaa acagatctgc
    14761 cgtttgctgc tgtaaggcgg caacctcggc gttaaccggt ttgatggtgg taggagggcg
    14821 atgatctaag taagaggcaa tcaccggtgc cggtaagcca atcgtttttg ccagtaaagc
    14881 gatgctttgt tcgcgctggc tgcgggttaa cgcatcggcc tcactaaagg ttgccagtac
    14941 gccctgaata aaagcgccgt ttttttctgc atagggacga gctgccagat aaaacgatcc
    15001 agtttgattg agatcggtgc cgtctttcag cacccgcacg ccgccctgta ataacgcagc
    15061 ggagtagtag ggatcccaga tagcccaggc gtcaacgtta ccttgctgga acgcggcgcg
    15121 ggcaccggca ggcgtcaggt aagtgggctg gatatcggtg aatttaagtc cggcctgacg
    15181 cagtgcacgc agtaaaaggt tgtgtgaact ggaacctttc tgaaaggcaa ctttgtgacc
    15241 tttaagatcg gctacggttt tgatcgggct gttttctgcc accagaatca cttcggcttt
    15301 gggcttcggt ggctcgacgc ccacatatac caaatcagcc ccggcagcct gggcaaagat
    15361 tggcggaata tccccggtac tgccgagatc aatactgcca acgtttaacg cttccaacat
    15421 ttgcggaccc gcggggaact ccacccagga gatttttgat tgcggatagc gtttttccag
    15481 taactggtgg ctttttgcca gcaccatacc aatactgcct ttctgatagc ctatacgtaa
    15541 cgcttcaggc gaggattctg cagcaaccgc aaaagtagag acgctaagca atcccgccag
    15601 cgccagttta atgatgttac gcatgggcat tacctcgcag agacagaagg tcaggaacct
    15661 gaacatcgcg gcggtgcaat gcctgccaga aagtttctag cgcggtatca agatggggtt
    15721 gcagatttgg cgtgaactgg ggtctgtgat ggtaatcaat taattgtgag tcatcggcaa
    15781 acacgccgtg caggatctcc tgagctttca gtgcgcttaa aactggttta agggcataat
    15841 cgaccgccag cagatgggcc acggtaccgc ccgtcgccag cggtagcacc actttgcctt
    15901 gcaaagcgcg ctctggcagc aggtcgagca gggt
//
LOCUS       DAJTYI010000089        15933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYI010000089 DAJTYI010000000
VERSION     DAJTYI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1682)
                     /gene="speA"
                     /locus_tag="OO564_002713"
     CDS             complement(<1..1682)
                     /gene="speA"
                     /locus_tag="OO564_002713"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6653787.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLG"
     gene            complement(1691..1822)
                     /gene="yqgB"
                     /locus_tag="OO564_002714"
     CDS             complement(1691..1822)
                     /gene="yqgB"
                     /locus_tag="OO564_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6653788.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1958..2173
                     /locus_tag="OO564_002715"
     CDS             1958..2173
                     /locus_tag="OO564_002715"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653789.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2477..3631
                     /gene="metK"
                     /locus_tag="OO564_002716"
     CDS             2477..3631
                     /gene="metK"
                     /locus_tag="OO564_002716"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6653790.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4068..5462
                     /gene="galP"
                     /locus_tag="OO564_002717"
     CDS             4068..5462
                     /gene="galP"
                     /locus_tag="OO564_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6653791.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVAFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5539..6036
                     /locus_tag="OO564_002718"
     CDS             5539..6036
                     /locus_tag="OO564_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6653792.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6131..6838
                     /gene="endA"
                     /locus_tag="OO564_002719"
     CDS             6131..6838
                     /gene="endA"
                     /locus_tag="OO564_002719"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6653793.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6918..7649
                     /gene="rsmE"
                     /locus_tag="OO564_002720"
     CDS             6918..7649
                     /gene="rsmE"
                     /locus_tag="OO564_002720"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6653794.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7662..8612
                     /gene="gshB"
                     /locus_tag="OO564_002721"
     CDS             7662..8612
                     /gene="gshB"
                     /locus_tag="OO564_002721"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6653795.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8721..9284
                     /locus_tag="OO564_002722"
     CDS             8721..9284
                     /locus_tag="OO564_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6653796.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9284..9700
                     /gene="ruvX"
                     /locus_tag="OO564_002723"
     CDS             9284..9700
                     /gene="ruvX"
                     /locus_tag="OO564_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6653797.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9876..10856)
                     /locus_tag="OO564_002724"
     CDS             complement(9876..10856)
                     /locus_tag="OO564_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6653798.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            10874..11578
                     /gene="yggS"
                     /locus_tag="OO564_002725"
     CDS             10874..11578
                     /gene="yggS"
                     /locus_tag="OO564_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6653799.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11596..12162
                     /gene="yggT"
                     /locus_tag="OO564_002726"
     CDS             11596..12162
                     /gene="yggT"
                     /locus_tag="OO564_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6653800.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12159..12449
                     /locus_tag="OO564_002727"
     CDS             12159..12449
                     /locus_tag="OO564_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6653801.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12457..13050
                     /locus_tag="OO564_002728"
     CDS             12457..13050
                     /locus_tag="OO564_002728"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6653802.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13043..14179
                     /gene="hemW"
                     /locus_tag="OO564_002729"
     CDS             13043..14179
                     /gene="hemW"
                     /locus_tag="OO564_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6653803.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14493..15456
                     /locus_tag="OO564_002730"
                     /pseudo
     CDS             14493..15456
                     /locus_tag="OO564_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15501..>15933
                     /locus_tag="OO564_002731"
     CDS             15501..>15933
                     /locus_tag="OO564_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6653804.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVM"
BASE COUNT         3926 a         4118 c         4028 g         3861 t
ORIGIN      
        1 ccgagcatcg gcggattctc tggatcgtac tccggcattg gcattgtcgt ggcaatacca
       61 tcaccatcaa tatagtggtc gatagcaccg tcagagtcac aggtaatatc cagcagcaca
      121 gcgcgacgtt ccggcacttg atccagccct tccagcggta gaaccgggaa caactggtcg
      181 atcccccatg cgtccggcat tgactggaac agcgagaaat tgacgtacat tttgtccgcc
      241 atacgttcct gcagttcgtc gattatcgga cgatgcgcac ggttttgcgg atccagttgc
      301 ttctgcactt catggcacat gctcagatag agttgttctg cccatgcacg ttcttgcagg
      361 ctaaaggtgc cggaagagta gccgatatga atgtcgtgca gatccatctg gctgtcgtgt
      421 aaccattcac gcaaagaacg gcgagttccc ggttcgtgca tttcctgcca ggtttcccac
      481 atgctttgca gcgcacgcgg cgcatcttct gcgggcgcgg tcggcaccgt atattcgtta
      541 cgttccacgc cgatgatatt cgacaccagc acggtatggt gcgcagtcac cgcacggccc
      601 gattcggtga ttaccgtcgg atgcggcaga ccattttctt cacacgcatc gccaatcgcc
      661 cagatgatgt tgttggcgta ttcattgagg ccatagttta cggagcagtc agactgcgaa
      721 cgggtgcctt cataatccac gcccagaccg ccgccgacgt cgaagcactg aatattgacg
      781 cccagcttgt gcagttctac atagaaacgc gcggattcac gaacgcctgt cgcgatatcg
      841 cgaatattcg ccatctgcga accgaggtgg aagtgcagta gttgcaggct gtcgagacgc
      901 ccggcttcac gcagggtttc aaccagttgc agtacctgag tcgcagccag gccgaacttc
      961 gatttttccc cgccggagga ctgccattta cccgagccct gcgaagccag acgtgcacgc
     1021 acgcccagac gaggaacgac attcagacgt tctgcttcat ccagcacaat ggcgatttct
     1081 gacatcttct caatgaccag atagaccttg tgccccatct tctcgccaat taatgccagg
     1141 cggatatatt cgcggtcttt ataaccgttg cagacgatga cgctacgagt catgccagca
     1201 tgtgccagca ctgccatcaa ctcggctttg gaaccggctt ccagacccag cggttcaccc
     1261 gaatgaatca gggactcgat cacgcggcgg tgctggttaa ctttgatcgg ataaacaagg
     1321 aagtaatcgc cgttatagcc gtaggattcc ctcgcacgtt tgaacgcggc gttaatggaa
     1381 cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg caggcagacg ctgaccctgt
     1441 gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag cttccgggac gtccgggtcc
     1501 gggcacacgc tgatgtggcc cagctcgtta acgtcatagt agttattgcc ccaccaggca
     1561 atattgtaag tacgcagcat cttgctggct tcctgggagc tcattgcaac ctcctgcatg
     1621 gagcgtagta caccgtgttc gcccgctgac gaaggcaaac ccatagacat gtcgtcagac
     1681 atagcgaacc tcaaattatt ttattaagtg taaaacagtt aacgactgtc gcagcctgga
     1741 actgcgataa caacccataa gcacatggat tttccagcag tgtatgctga cgctccgact
     1801 gcgcaaccgg tttctttttc ataacattat taagcacata accgaacgta agtgtgacag
     1861 ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc gcccttgttc agtcctcagt
     1921 aactgtaacc agctcttgaa tcctgagaag cgccgagatg ggtataacat cggcaggtat
     1981 gcaaagcaga gatgcagagt gcggggaacg aatcttcacc agaacggtga gacaggttaa
     2041 gcagcagaca acggttcatt atttcgtatc acctccacgg ccgcctgtta agacgaaccc
     2101 acaagccaaa actctgattt caacccggct ggaagtggca acacgaaaaa aacgtcgtgt
     2161 gctttttatt taagccgcgc gccgcgtttt ataccccaca atggcagaaa attgcaaaag
     2221 ataaatacgc agaatgccgg cattgtcagg aaaaatttcc agccacgttt ttaacagaat
     2281 gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat tgacctaaaa tagccatcca
     2341 gatgttaatc catccatacc gattaacact cagactgcca gtgtttttaa cctgcagagt
     2401 cgtggtagga tccgctacca cagaaaatcc acacaacagt ttgagctaac caaattctct
     2461 ttaggtgata ttaaatatgg caaaacacct ttttacgtcc gagtccgtct ctgaagggca
     2521 tcctgacaaa attgctgacc aaatctctga tgccgtttta gacgcgatcc tcgaacagga
     2581 tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc ggcatggttt tagttggcgg
     2641 cgaaatcacc accagcgcct gggtagacat cgaagagatc acccgtaaca ccgttcgcga
     2701 aattggctat gtgcattccg acatgggctt tgacgctaac tcctgtgcgg ttctgagcgc
     2761 tatcggcaaa cagtctcctg acatcaacca gggcgttgac cgtgccgatc cgctggaaca
     2821 gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact aatgaaaccg acgtgctgat
     2881 gccagcacct atcacctatg cacaccgtct ggtacagcgt caggctgaag tgcgtaaaaa
     2941 cggcactctg ccgtggctgc gcccggacgc gaaaagccag gtgactttcc agtatgacga
     3001 cggcaaaatc gttggtattg atgctgtcgt gctttccact cagcactctg aagagatcga
     3061 ccagaaatcg ctgcaagaag cggtaatgga agagatcatc aagccaattc tgcccgctga
     3121 atggctgact tctgccacca aattcttcat caacccgacc ggtcgtttcg ttatcggtgg
     3181 cccaatgggt gactgcggtc tgactggtcg taaaattatc gttgatacct acggcggcat
     3241 ggcgcgtcac ggtggcggtg cattctctgg taaagatcca tcaaaagtgg accgttccgc
     3301 agcctacgca gcacgttatg tcgcgaaaaa catcgttgct gctggcctgg ccgatcgttg
     3361 tgaaattcag gtttcctacg caatcggcgt ggctgaaccg acctccatca tggtagaaac
     3421 tttcggtact gagaaagtgc cttctgaaca actgactctg ctggtacgtg agttcttcga
     3481 cctgcgccca tacggtctga ttcagatgct ggatctgctg cacccgatct acaaagaaac
     3541 cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg gaaaaaaccg acaaagcgca
     3601 gctgctgcgc gatgctgccg gcctgaagta atctttcttc acctgcgttc aaaggccagc
     3661 ctcgcgctgg cctttttctt ttacctcacc gcggtggata ggcgttcacg ccgcatccgg
     3721 caaaaaaact gcccgcacaa taacatcatt cttcctgatc acgtttcacg gcaaattacc
     3781 atcacaattg aaaccgatta caccaaccac accagcaaaa tatttgtaat attttcatat
     3841 tattattcag ctttcacagt tgttacattt cttttcagta aagtcttaat tgcagatgac
     3901 agcgtttaat ctatgatgat ataactcaat tattttcatg cacttaaatc ataactaaga
     3961 taaatgttag tgtaagcgat tacactgatg tgatttgctt cacatctttt tacgtcgtac
     4021 tcacctatct taattcacaa taaaaaataa ccatattgga gggcatcatg cctgacgcta
     4081 aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt ctgcttcctt gccgctctgg
     4141 cgggattact ctttggcctg gatatcggtg taattgctgg cgcactgccg tttattgcag
     4201 atgaattcca gataacttcg cacacgcaag aatgggtcgt aagctccatg atgttcggtg
     4261 cggcagtcgg tgcggtgggc atcggctggc tctcctttaa actcgggcgc aaaaagagcc
     4321 tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt ctctgcggct gcgccaaacg
     4381 ttgaagtact gattctttcg cgcgttctgc tggggctggc ggtgggtgtg gcctcttata
     4441 ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat tcgcggcagt atgatctcga
     4501 tgtatcagtt gatgatcact atcgggatcc tcggtgctta tctttctgat accgccttca
     4561 gctacaccgg tgcatggcgc tggatgctgg gtgtgattat catcccggca attttgctgc
     4621 tgattggtgt cttcttcctg ccagacagcc cacgttggtt tgccgccaaa cgccgttttg
     4681 ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac
     4741 tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga
     4801 atagcaactt ccgccgcgcg gtgttccttg gcgtactgtt acaggtaatg cagcaattca
     4861 ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt cgaactggcg ggttatacca
     4921 acaccaccga gcaaatgtgg aggacagtga ttgtcggcct gaccaacgta cttgccacct
     4981 ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc aacgctaacg ctgggcttcc
     5041 tggtgatggc tgctggcatg ggcgtactcg gtacaatgat gcatatcggt atccactctc
     5101 cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt tattgtcgct tttgccatga
     5161 gtgccggtcc gctgatttgg gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt
     5221 ttggcatcac ctgctccacc gccaccaact ggattgccaa catgatcgtt ggcgcaacgt
     5281 tcctgaccat gctcaacacg ctgggtaacg ccaacacctt ctgggtgtat gcggctctga
     5341 acgtactgtt tatcctgctg acattgtggc tggtaccgga aaccaaacac gtttcgctgg
     5401 aacatattga acgtaatctg atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt
     5461 aatctcctca ggcttcctcc cgtctcggag gaagccgcct cttgcagtca tcttttcttc
     5521 gctctatcct ctgccgctat gaaaacatcc cgtctcccta tagccatcca acaggccgtt
     5581 atgcgccgcc tgcgggaaaa actcgcccag gccaacctga agctagggcg taactacccg
     5641 gagccaaaac tctcttacac ccagcgcgga acctccgccg gaacagcctg gctggaaagc
     5701 tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa
     5761 gtggtaccgc acgaactggc acatttgctg gtatggaaac atttcggccg cgtagcgcca
     5821 catggcaaag agtggaagtg gatgatggaa agcgtgctgg gcgttcccgc ccgtcgtacg
     5881 catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc cctaccgctg taagtgccag
     5941 gaacatcagc ttaccgtacg ccgccataat cgcgtagttc gtggcgaggc catctatcgc
     6001 tgtgttcact gcggtgaaca gctggttgcg aaataaccac ctgaactatc aggaactttc
     6061 ctgatctggc tgattgcata ccaaaacaac tttcgctacg ttgctggctc gttttaacac
     6121 ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg gtactgagcg cagcattttc
     6181 cggcccgacg ttggccgaag gtatcaatag tttttctcag gcgaaagccg cggcggtaaa
     6241 agtccacgct gatgcgcccg gtacgtttta ttgcggatgt aaaattaact ggcagggcaa
     6301 aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgcgc
     6361 cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa ttcggtcacc agcgccagtg
     6421 ctggcaggac ggtggacgta aaaactgcgc taaagatccg gtctatcgca agatggaaag
     6481 cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat ggcgatcgcg gcaattttat
     6541 gtacagccag tggaatggcg gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt
     6601 caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt gccattgcgc gcacctactt
     6661 ctatatgcgc gaccaataca acctgacact ctctcgccag caaacgcagc tgttcaacgc
     6721 atggaacaag atgtatccgg ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa
     6781 ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc
     6841 tacactagcg ggattctttt tgttaacccc taccccacgc gtacaaccgc gtggggagac
     6901 gacgcggatt tttaactatg cgtatccccc gcatttatca tcctgaacca ctgaccagcc
     6961 attctcacat cgcgctttgc gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg
     7021 ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca ggtctttgac gccgaaatta
     7081 ccagcgccag caaaaaaagc gtggaagtga aggtgctgga aggccagatc gacgatcgcg
     7141 aatctccgct gcatattcac ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta
     7201 ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac gccacttttt tctgagcgct
     7261 gcggcgttaa actggatagt gaacgtctga acaagaagct tcagcagtgg cagaagattg
     7321 caattgctgc ctgtgagcag tgtggtcgta accgggtgcc ggaaatccgt ccagcgatgg
     7381 atctggaagc ctggtgtgca gagcaggatg aaggactgaa actgaatctt cacccgcgcg
     7441 ccagtaacag catcaatacg ttgcctttac cggttgaacg cgtccgcctg ctgattggcc
     7501 cggaaggcgg tttatcggca gatgaaattg ccatgactgc ccgctatcaa tttactgata
     7561 tcctgttggg acctcgcgtt ttgcgtacag agacaactgc gctcaccgcc attaccgcgc
     7621 tacaagtacg atttggcgat ttgggctaac ggagaagaat aatgatcaag ctcggcatcg
     7681 tgatggaccc catcgcaaac atcaacatca agaaagattc cagtttcgct atgttgctgg
     7741 aagcacagcg tcgtggttac gaacttcact atatggagat gggcgatctg tatctgatca
     7801 atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa gcagaactac gaagagtggt
     7861 tttcgttcgt cggtgaacag gatctgccgc tggccgatct cgatgtgatc ctgatgcgta
     7921 aagacccgcc gtttgatacc gagtttatct acgcgaccta tattctggaa cgtgccgaag
     7981 agaaagggac gctgatcgtt aacaagccgc agagcctgcg cgactgtaac gagaaactgt
     8041 ttaccgcctg gttctctgac ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc
     8101 taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa gccgctggac ggtatgggcg
     8161 gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc
     8221 tgactgagca tggcactcgc tactgcatgg cgcaaaatta cctgccagcc attaaagatg
     8281 gcgacaaacg cgtgctggtg gtggatggcg agccggtacc gtactgcctg gcgcgtattc
     8341 cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc
     8401 tgacggaaag tgactggaaa atcgcccgtc agatcgggcc gacgctgaaa gaaaaagggc
     8461 tgatttttgt tggtctggat atcatcggcg accgtctgac tgaaattaac gtcaccagcc
     8521 caacctgtat tcgtgagatt gaagcagagt ttccggtgtc gatcaccgga atgttaatgg
     8581 atgccatcga agcacgttta cagcagcagt aacccacctt agcgagaagg atctcgttga
     8641 gactctgagt gacagcgccc ttctttccac gcatactggg cgctgttgct tttttgaacc
     8701 aggaaacaga acctctgaca atgaatttac agcatcactt tcttattgcc atgcctgctc
     8761 tccaggatcc gattttccgt cgttccgtgg tctacatttg cgaacataat accaatggtg
     8821 caatggggat catcgtcaac aagccgctgg aaaatctcaa aattgaaggg attctggaaa
     8881 agctgaagat cacgccggag ccgcgtgatg aatcaatccg tctggataaa ccggttatgc
     8941 tcggcggccc actggctgaa gatcgcgggt ttattttgca tactccgccc tccaattttg
     9001 cttccagcat tcgcatttca gacaacacgg tgatgaccac ttcccgcgat gtgctggaaa
     9061 cgctcggcac cgataaacaa ccgtctgacg tattggtggc tctgggctat gcctcctggg
     9121 aaaaaggtca actggaacaa gaaattctcg ataacgcgtg gctaacggcc ccggcggatc
     9181 tgaatattct gttcaaaacg ccgattgccg accgctggcg cgaggcggca aaactgattg
     9241 gtgtggatat tctcaccatg cctggtgtgg caggacacgc ctgatgagtg gaaccttact
     9301 cgccttcgac ttcggcacca aaagcattgg cgtagcggtc ggccaacgca ttaccggcac
     9361 cgctcgccct ttgcctgcaa ttaaagcgca ggacggtacg ccggactgga acctcatcga
     9421 acgtttactg aaagagtggc agcctgacga aatcatcgtc ggtttgccgc tgaatatgga
     9481 cggcaccgaa cagccgttaa ccgccagagc gcgcaaattt gccaaccgta ttcatggtcg
     9541 tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc actgtggaag cccgttccgg
     9601 tctttttgaa cagggcggct atcgggcgct caacaaaggc aaagttgact ctgcctctgc
     9661 ggttattatt ctcgaaagct atttcgagca gggatattaa ggcgatctaa aagcctggcg
     9721 gatagtcgtt accgccatct tgccggatgc ggcgtgaacg ccttatccag cacacatctg
     9781 gcagcggctg taggtctgat aagacgcgtt agcgtcgcat cagacattta ttgcctttat
     9841 aggccctata aacttgcgca tcgggcatgg caacgtcaca aacgcccttc ccccacccgc
     9901 tgctgataac tctgctgaaa cgttaacatc cccacctgct gcccggtttg aataacatgc
     9961 ggtaactggt gggttttccc ttcgcgaatc aaattcccca ccgcgggagt gttaatcagt
    10021 aattcaaaca gcgccacgcg tccttcctgt ttatccactt ccagcttttg tgacagcact
    10081 gcccgtaaac tacccgcaag ttgattacgt acgggatctt tttcctgcgc cggaaatgaa
    10141 tccaccagtc gctcaactgc ctgcgcggca ccacgtgtat gtaaggttgc cagcaccaga
    10201 tgcccggttt ctgccgccgt cagcgccaga cggattgtct cactgtcacg tagctctccg
    10261 agcaaaatca catcaggatc ttcccgcaat gcagcccgca accccgatgc gaacgtcata
    10321 cagtgcaaac caatttcccg ctgctggatc aaacatcgct ggctggcata gagatattcc
    10381 acaggatctt ccagcgtcag aatatgcgca tcggcatgtt gattaagata gccaaccatc
    10441 gccgccagcg tggtagattt gccactcccc gtagcccccg tcaccagaat caggccattc
    10501 tcgctcttga gtaattccgg caataccggt ggcgcaccaa gctgttcgag ctgtggacag
    10561 tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca
    10621 cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca gctggccatt ttccagcaat
    10681 attgcccgct gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcatca
    10741 aacggcgcag cttccattct gccacgaatg cgccatcgtg cgggccaggc gctgcacagg
    10801 tgtagatccg agacgttatg ctttacacta agggccacaa tttcttccat attcatacta
    10861 agatcctcgg aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc
    10921 agcggctgca acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa
    10981 aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga
    11041 aaactacgtt caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg
    11101 attagaatgg cattttattg gcccgttgca gtctaataaa agccgcctag tggcagagca
    11161 tttcgactgg tgtcatacca tcgaccgttt gcgcattgct acccgcctca acgatcagcg
    11221 cccggcagaa cttccccctc ttaacgtact gattcaaatt aatattagtg atgaaaacag
    11281 taagtccggg attcaactgg cagaactgga cgagctggca gcggcggtcg ctgaactacc
    11341 gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct gagtcagaat atgtaaggca
    11401 gtttgaagtt gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc gctacccgca
    11461 tatcgacacg ctctctctgg gaatgtcgga cgatatggaa gccgccattg cggcaggtag
    11521 cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt gattactcta aaaaataagg
    11581 aattaaagga acgccatgaa tacgttgact ttcctgcttt caacggtcat tgagctgtat
    11641 accatggtgc tgttattacg catctggatg cagtgggctc attgtgattt ttacaacccc
    11701 ttctcacagt ttgtagtgaa ggtagcgcag ccaattatcg ggccactgcg ccgcgttatt
    11761 ccggcaatgg gtcccattga cagcgcctcg ctgctggttg cctatattct cagttttatc
    11821 aaagccattg tgctgtttga agtggtgacc ttcctgccaa tcatctggat tgccggttta
    11881 ctgattctgc tgaaaaccat cggcttgctg attttctggg tcctgctggt gatggcgatt
    11941 atgagctggg taagccaggg tcgtagcccg attgaatacg tgctgattca gctggccgat
    12001 ccgctgctgc gcccgattcg ccgcttgcta ccggcaatgg gtgggattga tttctcgccg
    12061 atgatcctcg ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt attacaggca
    12121 accggaaaca tgctgctgcc ggggctgtgg atggcgttat gagtgccgta acagttaatg
    12181 atgacggtct ggttttacgg ctctatattc agccgaaagc cagccgtgat tctattgtcg
    12241 gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc gccggttgac ggccaggcca
    12301 acaatcatct ggtgaagttt ctcggtaagc aattccgggt tgccaaaagc caggtggtga
    12361 ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat cattaatccg caacaaatcc
    12421 cgccagaaat cgcggcgtta attaattagg tatcctatgc aaaaagttgt cctcgcaacc
    12481 ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta gcgactttgg tcttgatatc
    12541 gtggcccaaa cagacctcgg cgttgattcc gctgaagaaa ccggcctaac ctttatcgaa
    12601 aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg gtttaccggc aattgccgac
    12661 gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg gtatttactc cgcgcgttat
    12721 tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac tgttggaaac actgaaagac
    12781 gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg
    12841 gaagatccga ctccgctggt atgccacggc agctggccgg gcgtgattac tcacgaacca
    12901 gcgggcactg gtggctttgg ttatgatcca atcttcttcg taccttccga agggaaaacc
    12961 gccgccgaac tgacccgcga agaaaagagc gccatttccc accgtggtca ggcgttgaaa
    13021 ctgctgctgg acgctttacg taatggttaa attaccgccg ctgagtctct acattcacat
    13081 cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga
    13141 agtgccgcac gacgattatg ttcagcatct gctttgcgat ctggacaatg atgtggctta
    13201 cgctcagggc cgtgaagtaa agacaatctt tattggcggt ggtacgccga gcctgctttc
    13261 cggcccggcg atgcaaacgc tgctagacgg cgtgcgttcg cgtttgccgc tggcagcgga
    13321 tgcagaaatt actatggaag cgaacccagg cacggtagaa gcggatcgct ttgtcgatta
    13381 tcagcgtgct ggtgtaaacc gcatctctat tggtgtgcag agttttagcg aagaaaagct
    13441 gaaacgactt gggcgtattc atggcccgca agaagcgaaa cgagcggcaa agctggcgag
    13501 cggtttaggg ttacgtagct ttaaccttga tttgatgcat ggactaccgg atcaatcact
    13561 ggaagaggcg cttggcgatc tacgccaggc cattgaactg aatccgccgc atctttcctg
    13621 gtatcaactg accatcgaac ctaatacgct gtttggctcg cgaccgccgg tgctgccgga
    13681 cgatgacgcg ctgtgggata ttttcgaaca ggggcatcag ttattaaccg cagcgggtta
    13741 tcagcaatat gaaacttccg cttacgccaa acccggttat cagtgccagc acaatctcaa
    13801 ctactggcgc tttggtgact acatcggtat tggctgcggc gcgcacggca aagtgacctt
    13861 cccggatggg cgcattctgc gtaccactaa aacgcgtcat cctcgtggtt ttatgcaagg
    13921 aaggtatctg gaaagccagc gtgatgtcga agccgcagat aagccgtttg agttctttat
    13981 gaatcgcttc cgtctgctgg aggccgcgcc gcgcgtggag tttattgcgt ataccgggct
    14041 ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt gcccagggtt atctcaccga
    14101 atgtgcggat tactggcaga taacggaaca tggtaaatta ttcttaaact cacttcttga
    14161 attattcctc gctgagtaat cattaatgcg cctgcgggcg cattaatcac ttcttttatc
    14221 cccccgttaa ttaacctcgg cagaaatata tttcttactg aataaatatc gacgacattt
    14281 attatatata tttagtgaat atttactaat cattcattga ttataaatat gtacaacctg
    14341 gcgcacaaat ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag
    14401 ttgtcagaca ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa
    14461 ccaatttcca tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat
    14521 tagtctgcgc atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga
    14581 cgttaaagtt agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc
    14641 tggcgaaaga ggtaaaacag ctgtcaaaag gtaatatggt gatccgtatt tatcccagca
    14701 gccagatggg taatgcgcga gaaactatgg aattgttgca aaatggcgca ctggatatga
    14761 ccaaaggttc cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc
    14821 cgttcttatt aagttgtttt tggtgaagtt ggtaaagaaa ttatggactc aacaaaagac
    14881 aaaggctttt ttgctttatc agcttatgta gcgggtactc gcagtttcta tgcgaaaaaa
    14941 ccgattacaa agccagaaga tcttaaagga ctgaaaattc gtgttcagcc aagtccgacc
    15001 accatcaaaa tgattgaact gatgggtggt tcacccactc caatttcatt tggcgaagtt
    15061 tatactgcga tgcagcaagg tgtagtagat ggcgcagaaa ataacgtgcc ttcctggatg
    15121 cagactcgac atattgaaat tgccaaagtt ttttctgaag atgaacacgc ctcaattcct
    15181 gactttttgg ttatctccac caaaacatgg aataaattac ccccagaaca gcaacaaatc
    15241 ctcgaaacag cggcgaagaa atccgaagct tatcaacaaa aattgtggga aaaaattgac
    15301 gctgacacac gtgctcaggc gaaagctatg gggggtgaaa ttgtaaaagt ggataaagcg
    15361 ccattccgta aagccgtgca acctctgttt gacgacttca aaaaagatcc aaaacaagct
    15421 gctttgttag agaaattcga caacgctgct caataatatt ccgggccagc ctctggccct
    15481 gattttccag gatgcttaca atgatattta accgcctgaa acttgcggta gaccgcgtca
    15541 ttgccgcgtt ttctgtcgcc gtaatgcttg ctctggttgt atgcgtagtc tggcaagtat
    15601 ttagccgcta cgtacttaac cagccaagta cattaaccga tgagctggca cgctttttga
    15661 tgatctgggt aggattatta ggtgctgcat ataccgtcgg cgcgcagcga catctctcta
    15721 ttgatttatt tgctctcgcc ctgaataagc gcaaacaatt acttcttagt atagtcatta
    15781 acgttctcat tttaggtttt gccggttcgg tcattgtgac tggtggcctg aaattaattg
    15841 ataaaacact ggcaacatca caggtatctg ccgctatgca aataccgatg ggatacgtat
    15901 atatcattct gccattaagt ggagtggtaa tga
//
LOCUS       DAJTYI010000090        15693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYI010000090 DAJTYI010000000
VERSION     DAJTYI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..387)
                     /locus_tag="OO564_002732"
     CDS             complement(<1..387)
                     /locus_tag="OO564_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6653805.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSC"
     gene            complement(384..3068)
                     /locus_tag="OO564_002733"
     CDS             complement(384..3068)
                     /locus_tag="OO564_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6653806.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3160..3660)
                     /locus_tag="OO564_002734"
     CDS             complement(3160..3660)
                     /locus_tag="OO564_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6653807.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3690..4406)
                     /locus_tag="OO564_002735"
     CDS             complement(3690..4406)
                     /locus_tag="OO564_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6653808.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAKKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4702..5478
                     /gene="exuR"
                     /locus_tag="OO564_002736"
     CDS             4702..5478
                     /gene="exuR"
                     /locus_tag="OO564_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6653809.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5823..6485
                     /locus_tag="OO564_002737"
     CDS             5823..6485
                     /locus_tag="OO564_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6653810.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6489..6872
                     /gene="mzrA"
                     /locus_tag="OO564_002738"
     CDS             6489..6872
                     /gene="mzrA"
                     /locus_tag="OO564_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6653811.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7019..7373
                     /locus_tag="OO564_002739"
                     /pseudo
     CDS             7019..7373
                     /locus_tag="OO564_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7411..7716
                     /locus_tag="OO564_002740"
     CDS             7411..7716
                     /locus_tag="OO564_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6653812.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7719..8123
                     /locus_tag="OO564_002741"
     CDS             7719..8123
                     /locus_tag="OO564_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6653813.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8113..8412
                     /locus_tag="OO564_002742"
     CDS             8113..8412
                     /locus_tag="OO564_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6653814.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8598..8990
                     /locus_tag="OO564_002743"
     CDS             8598..8990
                     /locus_tag="OO564_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6653815.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLARTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            9060..10046
                     /gene="yqjG"
                     /locus_tag="OO564_002744"
     CDS             9060..10046
                     /gene="yqjG"
                     /locus_tag="OO564_002744"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6653816.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10339..10707
                     /locus_tag="OO564_002745"
     CDS             10339..10707
                     /locus_tag="OO564_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6653817.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10914..11806)
                     /gene="yhaJ"
                     /locus_tag="OO564_002746"
                     /pseudo
     CDS             complement(10914..11806)
                     /gene="yhaJ"
                     /locus_tag="OO564_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            11911..12612
                     /locus_tag="OO564_002747"
     CDS             11911..12612
                     /locus_tag="OO564_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6653818.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12635..12799
                     /locus_tag="OO564_002748"
     CDS             12635..12799
                     /locus_tag="OO564_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653819.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13011..14303)
                     /locus_tag="OO564_002749"
     CDS             complement(13011..14303)
                     /locus_tag="OO564_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6653820.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14331..15662)
                     /locus_tag="OO564_002750"
     CDS             complement(14331..15662)
                     /locus_tag="OO564_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6653821.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4068 a         4007 c         3639 g         3979 t
ORIGIN      
        1 acatgaagat ctattagaat ccttaagatt cgtctgaagc cctttgcccc ccttgcatcc
       61 tagcggcgct tcatcgctac ttacaatggc tgttttaaat cctttgagtt ttaccggcca
      121 acgcatccca ctgcgttgtt cagttaaata gatttgtact gtcgatatat ttccattagc
      181 tgtacaggtt tgttggaggt caggatacga acattcatac cacatatatc cattatttcc
      241 tgccgacgct tcatccttcc agaagtaata atccgctcta ccagatgcca aatcatacgt
      301 aatttcactg gtcacgtcag gagctgcctg aacgccagtg agcgtgccaa acaagcccaa
      361 tatcgccgca atcaatcgat ttctcatggt gcaacctccc tttgcgccgt ctgaacgttt
      421 ccacgtaatt taggctgttt caactgtgcc tgaacctgaa tcgactccgg taattgtgaa
      481 taagtcacca cactacaagt aacatctccg ctgtaacgaa tgctggcgcg tttctttaca
      541 acactcagtg gacacttaag gaattgctgc ccggaaagca gatagagctc tttttcgctg
      601 ccgttaattt caagtgtaaa tccgccatct tcagctacca gtaacatggc gctgttcagc
      661 cccacaatcg ggaatttttt caccgctgcc ggcagtaata aacgaccgat atagttgtag
      721 ttaaagctgg cagacacttc gcgatagcgc agcttgccag gcaacaacat taattcacga
      781 ttccctgcac cgttaatgat gtttgttgtc ccctgctgat tgccctggct actgtcgtta
      841 acctcaacaa aaccagactg aaatccagga taagggaaca acacactgtc actttgacca
      901 agctgcaaag ttcggctgcc tgccacccgc gcttcgagtt gcagatcctg actctcttca
      961 tcctgttcag agaagccttt cacatccacc agtaccgcac ccagcaaatc gtctgtacca
     1021 ctggctccca aattaacgcc atagcgactg acagccagcg tagagctata agtgcccgta
     1081 aaggccgaaa gatgatcatg attcttacgg tcataactat cagatacggt agcgtagaca
     1141 tttccgtatt gcccatccca acgcccgtta accgcgctgt ttatggtatc ggtgttcagg
     1201 cctccgacgg ttacgccaag ttctttatgg ctgaatgaat cgttataaaa ggtatgcgat
     1261 aactgccatg acgtttgatc gccatcctgt tcgctataac gataatccgt agacacattg
     1321 gttgaatggc tgttgttatt gctatccatc gttggcgtgt cagataacga aaacgataag
     1381 tacaagccgt tgtcgtcgtt atcgccaccg ttgtcattac gatggaaaac accgactgac
     1441 gtactcaaaa tccagtcacg catcacaata gcgcgggaag cgctaagttg tacagtttca
     1501 gagatacttt gcgctgttgt ctggcgaaga tagttatttt caaagctctg ctgtttatcc
     1561 caacgcaaac tatcatcagt gttattttgt gtgcaggtat aacccagcat catattccag
     1621 ccgacaaaat tcagtgaaac tgtcgcggaa atactttcat aacagcttaa ggcgttatag
     1681 ctctctctgc tgtcggttgt acaagcatcg ccgtcagagt tggtccgata aaagcccaac
     1741 gtcggccatc ccggattact ccagttggcc tgttgcatat cacctttgcc gccgtcatcg
     1801 ttacggaaca cgctggcgct gattgcaaga ttccccaccc cgcctaaatc tgccgtccag
     1861 ttattaccta actcgaaagc actcacatca tcggcattcg ccagccctgc gtagagctca
     1921 tattgcggat gtaatggcag gcgtactcct agctgataag ctgaactttc atcatcagaa
     1981 acctgtgatg tagttttacc tgcctgtaag aaccattgcg catttccgtc agtcagaccg
     2041 ccggttttgg taaacggcac aagctcggtg cgggtgagtt ggttattttc gtagactttt
     2101 aatgccacgc tgtagctgcc tggcggaaag gaactggtat caataaattg cgaaccacta
     2161 ttgagataaa acgatcccaa aagttgctca ttacgataag cgtcaacacg agaattgcgc
     2221 gaaagcagaa ccattactgg ggttccttgc tggctttgcg cctggtttaa atagctcagt
     2281 gtcgacccga tacgcatccc atcgattgca ccgagtggca gaaagttaaa ggtaaagttc
     2341 ccgccttgtg cattaaatag tgtgcggttg tccatgcgac ccgcctgcac ataataacga
     2401 cgtaaaaaat cataacgata atagagatcg ctgacgtcag tattactgtc actgacatca
     2461 tctgcatcat aaccgttgaa attccagtgt gcaccaatat agctattttc tgttattccc
     2521 agcgcaccgt ttccctgaat agacaacgat tggtaatcat cctgtgccag aacattgatg
     2581 tcttgctgat gtataaaagc gttaacagta tttttgtctg gatttaaata taatgacttc
     2641 gcatcgaaag ctgaattcca ttgcggatta ataaacaacg ttgccgaact ttcttcatca
     2701 ttatagatta tttccagcgt atcggtattc agaaaaccac accctgagtc agtacgtaca
     2761 taaccgcaag ccaattcacc gtggcgagct agcggctgcg ataatttttc tttcactata
     2821 tgcgcgattt tctgatcgtc tacatccagt tccagcttgt tatatagact tgctggatca
     2881 agaaatgtta cggtatcaag attaaccatt gcataatatt taccgagact tttccctgaa
     2941 aaataaacct cgatatactc ctgctgtccc tgagcaataa cctcaaaccc tgcgggaatt
     3001 ttggttaatg tcgcctctgc cgggctgaga ctcgccagga tcaaaagagc cagtaatttt
     3061 ttatccatgg caatcctgaa atccataaca aatactaaaa cagttaaaat aagggagggc
     3121 tacccctccc tttgttacgt gttaattctg ttatccggat taaatatcct gagacagata
     3181 aatgcttact acaccacggt agatgcctgt ttccagaaca cctttggtgg tttgagcaaa
     3241 catcaggttt ttagccagag aaccgttagt taacgcatcg gaagcaaaga ttttggttgc
     3301 ggtaaaagtg gttgccgcat cagctttaat ttcttcacca ccccaggtta ctgtcagcgg
     3361 aacaatcttg ctagcatcca ggctttgaac cagttgcgct ggggacacta actgcatttt
     3421 tacatctttg gtaacgtcgt tagaccagat tttggtcatc aactggtaag attgcagacc
     3481 ctgccccggc agatattgca tttctactgc tttcggcaac gcggtgttat cggtctgtgt
     3541 catatccaga gcggcatcaa cgttggccgt tacagtaata tccttttgta ctgccaatgc
     3601 ggagcctgca acagaaaaca tcgctgcgac cagaagagat tttgcaaata cctttttcat
     3661 tactattcct tcgtttatta caatgataat taatggaccg ccagttcaaa ctcgccatta
     3721 tctttggtga tccagttatt atatttgaca acgattttgt cgtagccaga tatgctttca
     3781 agatttcttt cctgaccagg gaatatgttt ttatttacgg tctgtatctt acagttttct
     3841 ttgctattat ttttacaata agcgatcgtt aaaagcttta agcgttgatt ccctgcatta
     3901 attaattttt tggcagaatt tacttctaac ttagctatag gttgctgagg tggaacacta
     3961 acaacgattc cccaaattaa gttaactgtt aactgcatag agagatcttt tttatctgtg
     4021 gcaacgtcat caatactggg aaccgcttca aatttaacgc gataattttt ctctttttgc
     4081 tctggttcca tcgcaacaaa acgtacggtt ttgctactac cggctggtaa agcgaattta
     4141 ggtggcataa caaccagatg atttgcatca ccggatttaa tctcaacttc attttcctga
     4201 ggcgtcgatg gattatcgat acggaatact gtcgccttga tgtactgaac ttcattactt
     4261 ttagatatta cgcgaacgtt gccttcaccc tgactattaa tggaaacagg cataggataa
     4321 acagtcatat ttgactgtgc caaaaaagga gcgaacacga ataaaagcgg cgtaagccgt
     4381 aataagagat taattttcgc caccatataa ttttgatctt acaatatcgt ttcaaaaaag
     4441 ttggtgtatt agagatgtag atttattgta agtcaatata aaaaagcatt tttagttaac
     4501 attaaattta aaggctggga attaacttac tgaaatttag tagctttcac aatgacgata
     4561 cttatttttt aggaatttat aaatattcac aatattaatt taatacatta attcaatcat
     4621 caaatttata tatgaccaat ggcataccca tgaataaagt ggtatgataa cttcgtccgc
     4681 attccactcc gggagacgca tatggaaatc actgaaccac gccgtttgta tcaacaactt
     4741 gctgctgacc tgaaagagcg cattgaacag ggcgtctatc tggtgggtga taaactgcct
     4801 gccgaacgct ttattgccga tgaaaagaac gtcagccgaa cggtagttcg tgaagccatc
     4861 atcatgctgg aagttgaagg ctatgtggag gtgcgtaaag gttcaggtat tcatgtggtt
     4921 tccaaccagc cgcgtcatca gcaggcggct gacaataata tggaattcgc caattacggt
     4981 ccgtttgagt tgcttcaggc tcgccagctc atcgaaagta atattgccga gttcgcggca
     5041 actcaggtaa cgaaacaaga catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc
     5101 gaacaatgct tccgtgattc cgagtgggat ttacagttcc acattcaggt agccctggcg
     5161 acgcagaact ccgccctggc ggccatcgtt gaaaaaatgt ggacccagcg tagccataac
     5221 ccgtactgga aaaaactaca cgaacacatt gattcccgta ccgttgataa ctggtgtgat
     5281 gaccacgatc aaattctcaa ggcgttaatt cgcaaagatc ctcatgctgc taagctggca
     5341 atgtggcagc atctggaaaa caccaaaatc atgttattta acgaaaccag cgacgatttc
     5401 gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tagtgcatct cgatacggcc
     5461 accagcggca gcaaatgaaa ttcctgtccg acaggcgctg caatgcgcct gtttgtctcg
     5521 tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc
     5581 aatcagcaaa atcagactcc acggacatgt aattttgata acgaacaacg ttgacctttg
     5641 ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc acgttttaac
     5701 agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa aacagcaata
     5761 acattgcggt agtgcatctt ttaaaaccag cgtggcgtta accgattcac caggaataat
     5821 gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg
     5881 ccaatccatc gatgattggc atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa
     5941 atggcttgct tccggcggcc tttttaccgg gcgacagttt actggtattg gtcggcgtgt
     6001 tgattgcgaa aggcgcgatg ggttacccgc aaacgattct gctgctgacc gttgccgcca
     6061 gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc cgcaccgtac
     6121 aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat ctttttcata
     6181 aacacggttt atcggcgctg ttaattggtc gctttattgc atttgtcaga acactgctgc
     6241 cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc aactggatga
     6301 gcggtctgct gtgggtattg atcctgacaa ctctgggtta catgctcggc aaaacgccgg
     6361 tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg gtggtgctgc
     6421 tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat ggaaatcggg
     6481 ggtaagggat gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt
     6541 tacttctggt cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc
     6601 tggcgattcg tgccgttcat caaggcacaa cgatgccaga cggtttttcg atctggcatc
     6661 accttgacgc tcatggcatt cctttcaaaa gtatcacccc caaaaacgac actctgttaa
     6721 ttacatttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc
     6781 cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag tggctgaccc
     6841 ggttacggga taactctcat cgcttcggat aacttcctgt tatccgaaac atatcactca
     6901 ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac actaccgctt
     6961 cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag gttcaagaat
     7021 gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta gttatgccac
     7081 taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg ccgaaaaaca
     7141 ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc gggccaactg
     7201 ttcagatagc cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga aagatgaggt
     7261 ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg atagaacgga
     7321 aactggcaga agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa
     7381 tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg
     7441 cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag
     7501 aagtcgaaag aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc
     7561 cgttatcgcc tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg
     7621 cgtgctgatg agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg cgctgcaatc
     7681 ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc
     7741 aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa
     7801 tggtggaaac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct
     7861 ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc
     7921 tgatggtgct aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca
     7981 ccaccgtggt gttgctgcta ctggcactga ttggcggtat ctggacgtta cgtaaatcgc
     8041 gtaaatccac cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg
     8101 aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc
     8161 cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca
     8221 ggcgcttacg atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc
     8281 agtagcgtga tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga
     8341 cgcggttttg gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag
     8401 cttcgcggtt aacttccctc tggccggagc aattccggcc ttatccctca aattttttga
     8461 atatttttga cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca
     8521 tcgacagcaa cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg
     8581 gcgcagtgga gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcacattt
     8641 taatgccgat tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc
     8701 aacaatatat ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgcttg
     8761 agttcggtgg tggtctggca atcctattcg gtttcctggc tcgcaccaca accctgttta
     8821 ctgcgggctt tacgctgctg acggcatttt tatttcacag caactttgct gaaggcgtca
     8881 actcgctgat gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta
     8941 ccggtccggg cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta
     9001 tacttaacga taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa
     9061 tgggtcaact gattgacggc gtctggcatg acacctggta cgataccaaa tctaccggcg
     9121 gtaaatttca acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcacctg
     9181 gccccactgg cacaggcggt tttatcgcag agaaagatcg ttatcatctc tatgtttcac
     9241 tcgcctgccc gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt
     9301 ttatttccgt ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca
     9361 gttttccggg agcaaccgga gatacgctct atcaacatga atttctgtat cagctttatc
     9421 tccacgccga tccacactac agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga
     9481 accacaccat cgtcagcaac gaatcagcag aaatcatccg catgttcaat accgcgtttg
     9541 atgcgctggg cgcaaaagcg ggtgattact acccaccagc cctgcaaacg aaaattgacg
     9601 aacttaacgg ctggatttat gacaccgtta acaacggcgt gtataaagct ggttttgcca
     9661 ccagccagca agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg
     9721 agcagatttt aggccagcat cgttacctga ccggcaacca gctaaccgaa gccgatattc
     9781 gcctgtggac tacgctggtg cgttttgatc cagtgtatgt gacccacttc aagtgtgata
     9841 agcaccgcat cagcgattac ctgaatctgt atggcttcct gcgcgatatc taccagatgc
     9901 cggggatcgc cgaaactgtc aatttcgatc acatccgtaa tcattacttc cgcagccata
     9961 agaccatcaa ccctacgggg attatttcaa ttggtccgtg gcaggatctc gatgaaccgc
    10021 atggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct ctgataatta
    10081 gctaagtgca tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac ctacaaagcg
    10141 tttatattca atatgttaca atgaccgtgt aggccagata agcgtagcgc atctggcgat
    10201 tttgcgcttg ccatcagtct tagcgcgcct tcacggcact ttattaattc cttgaacgaa
    10261 tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa caagtgattg
    10321 agcaaaattg aggcaaaaat ggactggtat ctgaaagtac taaaaaatta tgtcggtttc
    10381 cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat catctttacg
    10441 ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc
    10501 ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcggttca gttccgccgc
    10561 ctgcacgaca ccgaccgctc ggcgtggtgg gcactgctat tcttaatccc gtttatcggc
    10621 tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa ccgctttgga
    10681 ccagatccaa aactggaaca agaataatga ttaagcacct tgtaaaaggt gcttcaaatt
    10741 gttgacaaag tgcgctttgt ttatgccgga tgcggcgtaa acgccttatc cagcctacaa
    10801 attgccaaaa ttcaatatat tgcagtgact gcgtaggcct gataagcgta gcgcatcagg
    10861 cagttttgcg tttgtcagca gtcagaagca ccttgtgaaa ggtgcttaat agattatttt
    10921 ccagcgaaca actttggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca
    10981 cgacgccagg ccataataat atcgatctcg ctggtcgatt ccgggctgac gacacgcaac
    11041 cgcccttccg caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc
    11101 gccagtaatg cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc
    11161 agcagctgta cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga
    11221 tatttcacac gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc
    11281 gcggcaacgt agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa
    11341 cgaaaatgca tatccggcgc gataacgata tccgcccgcc cctgctccag ccgttcccac
    11401 gcccccgcca gcacttctgt gatgattgcc agttgggtat tggcttttgc cgccagtttg
    11461 tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc
    11521 gtttcccaac cgcgcgcgag agcttccgca tcagtagtca gtttatctgc ggcttccagc
    11581 aaaacgcgcc ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc
    11641 gagcggtcaa acagcaccac atccagctct tcttccagtt tttgcatgat gtagctaagt
    11701 gcggaaggca cgcgtcccag ctcatccgct gccgccgcaa aactgccccg gcgatcgcat
    11761 ccataacccg tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca
    11821 ggaaatttga acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc
    11881 ataaaaggaa gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt
    11941 ggacaagcag actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc
    12001 gacccgaaat tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca
    12061 ggtgccgcct ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat
    12121 ggagaagcag agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggagaggcg
    12181 ttgctgctct ctacccagcc gggtgtcagc tatagcgaac acaatctcag caaagacaaa
    12241 ccgttaacgc gaatgcagct ttggctggat acctgcccgc agcgagagaa tccgctgatt
    12301 caaaagctgg cgcttaatat ggacaagcag caattaatcg cctcgccaga gggtgcgatg
    12361 ggaagcctgc aattacgcca acaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa
    12421 agtgcgaatt tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt
    12481 catgcgctta cgcatcatga agagaaagcg gcgctaacct gcggtgatgg ggcgtttatt
    12541 cgtgacgagg ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat
    12601 ttgcctgtct agttgttttt acagggagat gatgatgagt aaaaaatcga ccaaaaagcg
    12661 ccagctagcg aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta
    12721 tgaagagatg ttgagcgagc tggaagctat cgtcgcggat gctgaaacgc gtttagccga
    12781 ggatgaagct accgcgtaaa gacaggttgc cggatgcggc gtaaacgcct tatccggcct
    12841 acaaaacaca acaacatcaa caaattaaac accccgtagg ccggataaga tgcgccagca
    12901 tcgcatccgg catcaggtac aaaacacaac aacatcaaca agttgagcac cccgtaggcc
    12961 ggataaggtg cgccagcatc gcatccggca caatactcaa aacctggcgt ttatctggcc
    13021 ttgctcgcca taatctcgat aatctgccga tccgtttgct gcatcgaatg gcttgctaac
    13081 gcacacaggt tggcaatcga ctgctcaaca tcatgcgcca cgatcccttc attgccggtc
    13141 acggcggtat catccagcgc cattaacacc gctttccacg cagccgaagc actggtcgaa
    13201 accttcgcac catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc
    13261 gagatagttt cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct
    13321 gcggtcgttg cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg
    13381 ctcaaatgag aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct
    13441 gctacaacca ccacaggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc
    13501 atagccggaa gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca
    13561 atggatgaag agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg
    13621 ccaatatgca gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc
    13681 gcggaatcga gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc
    13741 tcagccagcg tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca
    13801 cacgcctgct gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc
    13861 ccgccgacga tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag
    13921 aggatttcat tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct
    13981 ttggcatcgg caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca
    14041 tttccaccta acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga
    14101 acggtgatgc ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca
    14161 accggacctt ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca
    14221 gtacatccca gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac
    14281 ggatttaaag tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat
    14341 gccaggaaca gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc
    14401 cctttgtatt tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct
    14461 accatgccga aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg
    14521 atggcgctcc aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg
    14581 ttaatcttct cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct
    14641 tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg
    14701 acgctgacca ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc
    14761 agcgcggaaa tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg
    14821 aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc
    14881 cgcaatgctt tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc
    14941 atcgggctta acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg
    15001 gcgtttttca ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt
    15061 cccaccatcg acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc
    15121 gaaattttga ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt
    15181 accagaccat aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga
    15241 taggaagcac tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt
    15301 acgaaataga gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca
    15361 ctcgggtaat ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac
    15421 agatacattg ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa
    15481 ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc
    15541 acccagcagg tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt
    15601 ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc
    15661 ataatttctc gctcaatatt ttgtagggct tat
//
LOCUS       DAJTYI010000091        15536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYI010000091 DAJTYI010000000
VERSION     DAJTYI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..883)
                     /locus_tag="OO564_002751"
     CDS             complement(<1..883)
                     /locus_tag="OO564_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6653822.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            990..1286
                     /locus_tag="OO564_002752"
     CDS             990..1286
                     /locus_tag="OO564_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6653823.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1420..1845)
                     /gene="arsC"
                     /locus_tag="OO564_002753"
     CDS             complement(1420..1845)
                     /gene="arsC"
                     /locus_tag="OO564_002753"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6653824.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1858..3147)
                     /gene="arsB"
                     /locus_tag="OO564_002754"
     CDS             complement(1858..3147)
                     /gene="arsB"
                     /locus_tag="OO564_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6653825.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3201..3554)
                     /gene="arsR"
                     /locus_tag="OO564_002755"
     CDS             complement(3201..3554)
                     /gene="arsR"
                     /locus_tag="OO564_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6653826.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4299..4382
                     /locus_tag="OO564_002756"
     CDS             4299..4382
                     /locus_tag="OO564_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6653827.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4436..5788)
                     /gene="gorA"
                     /locus_tag="OO564_002757"
     CDS             complement(4436..5788)
                     /gene="gorA"
                     /locus_tag="OO564_002757"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6653828.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5860..6702)
                     /gene="rlmJ"
                     /locus_tag="OO564_002758"
     CDS             complement(5860..6702)
                     /gene="rlmJ"
                     /locus_tag="OO564_002758"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6653829.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6905..8947
                     /gene="prlC"
                     /locus_tag="OO564_002759"
     CDS             6905..8947
                     /gene="prlC"
                     /locus_tag="OO564_002759"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6653830.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8955..9707
                     /gene="rsmJ"
                     /locus_tag="OO564_002760"
     CDS             8955..9707
                     /gene="rsmJ"
                     /locus_tag="OO564_002760"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6653831.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9756..11225)
                     /gene="dtpB"
                     /locus_tag="OO564_002761"
                     /pseudo
     CDS             complement(9756..11225)
                     /gene="dtpB"
                     /locus_tag="OO564_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11531..11965)
                     /gene="uspA"
                     /locus_tag="OO564_002762"
     CDS             complement(11531..11965)
                     /gene="uspA"
                     /locus_tag="OO564_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6653832.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12356..12691
                     /gene="uspB"
                     /locus_tag="OO564_002763"
     CDS             12356..12691
                     /gene="uspB"
                     /locus_tag="OO564_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6653833.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12762..14261)
                     /gene="pitA"
                     /locus_tag="OO564_002764"
     CDS             complement(12762..14261)
                     /gene="pitA"
                     /locus_tag="OO564_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6653834.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14493..>15536
                     /locus_tag="OO564_002765"
     CDS             14493..>15536
                     /locus_tag="OO564_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6653835.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNE"
BASE COUNT         3927 a         3990 c         4069 g         3550 t
ORIGIN      
        1 ttgcgccggc actgccgatg attaatgcca ttaatgctcc cagacccatt ccttttgtca
       61 tcagaacaga tgccagaggg ataaccgcct cagcccggat atacagcgga ataccgacaa
      121 cggcgcttaa tgggatggcg aagggattat ctgctcctgc atgagcggca atccacgctg
      181 agggaataaa gccataaata aaagatccta ttaatactcc taacaaaaga tatggcagta
      241 catctttaaa ctgttgcaac gcatctttgg tggctagttt tatgggatta atacttacta
      301 cagctttggt actgcaacag gtaactacgg ggttctcaat ggctttagcc gctggactac
      361 aacaactcac ttttatgggc gtatatgtcg ttcccggcga agtttttgct ggagcacaac
      421 aatttgctga tgagctttta ctggcaatga tatgacgttc aaaccccaga gaatccagga
      481 taatactggc aagaacggag acgccggctg cgataatcgc gtacaacaag gtgactttcc
      541 aaccaaaggt catccacatt agtccgacga taatgggatt aagtaatggt gaaacaaata
      601 aaaaagtgag agtcggacca aacccggctt tcgctgacaa caatccacgt aacatgggta
      661 ttgtcgagca actacagaac ggggtaacgg ctcccaacag agcagccagg agatagcctc
      721 tcccttttct ggcccccatc atctgctgga ttttatggtc tggcaccttt tgtcttatca
      781 ggctgacacc ggcactaatc actataaaca gcaacgaaag ctcgacggcg agaaacacaa
      841 acatctctgc ggcatcttgc agcatcgcaa gccaactact catacgtaac ctcattgggt
      901 aaatctatat ttccagaata atcgaaatga aattcagatc aagatatttc tggtattatc
      961 gaaatatcaa ccagacagag gggtgtcata tgcagttgga agaagtcgct aaagcgctga
     1021 aagagctagg tcacccgacc cgtttgttca tatttaaaca tcttgtcaaa gccggggaac
     1081 aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat acctggctcc acactcagcc
     1141 accatatatc agcactggta tcggtaggac tggtcaaaca aaaccgtgag agtcggacgc
     1201 tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat tgaattctta cgcgaggagt
     1261 gctgcgtaaa tagcaaaatt gcctgatgcg ctacgctcta catatttcat gccatatatt
     1321 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
     1381 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
     1441 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
     1501 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
     1561 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
     1621 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
     1681 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
     1741 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
     1801 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
     1861 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
     1921 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
     1981 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
     2041 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
     2101 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
     2161 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
     2221 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
     2281 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     2341 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     2401 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     2461 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     2521 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     2581 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     2641 gccaaagaaa tcagcggaaa cgatattcac caagttggag acaataagcg gcaggctggc
     2701 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     2761 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     2821 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     2881 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     2941 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     3001 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     3061 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     3121 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     3181 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     3241 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     3301 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     3361 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     3421 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     3481 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     3541 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     3601 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     3661 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaaccgact
     3721 ttgcgtcggt ttttttactc ttcagcctga gttggtggct ctggctggag tgagaatgcc
     3781 cacatctaac ggttcaatac tattagccag tggctaacat tgcaagcgtt agcgatttct
     3841 tcagctggag aagggacagt ttgctataca tggtggtgaa agaggattta tatactttca
     3901 tcgggtcgct gccgtaccaa gccttgtccg aatccggacg taaaaaaacc gacttcgcgt
     3961 cggttttttt actcttcagc ccagagttgg tggctctggc tggagtgaga atgccatcat
     4021 ctaacgcatc aatgctaata ccatactgaa actattgcaa ggttatgctg gttatttagc
     4081 ctaattacac tgtatgatta tccagttagc tctgaggcat tttcactctg gcaatgcgca
     4141 taaacgcttt caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc
     4201 ggagtgagat tgcccacatc taacaatgag gcatgcgccg tgacaggcag tggatgagta
     4261 agcggatgca ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg
     4321 ttcttggggc gttaattgtg ctgctggaac tgatccgctt tctgcttcag cttctgaact
     4381 gatagcggaa acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac
     4441 gcatggtcac gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt
     4501 ttttggttgc gcccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa
     4561 agccaatgcc gtgaataccg acaatcttct cttctggccc aacgcacacc agcttcatgc
     4621 ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca
     4681 ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac
     4741 caatcggcgg atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg
     4801 gcttgttatt aaacaagcgt tcagagagac ggcgacccgc tgcaactgcc accggtgtca
     4861 gttccactgc acccgtgtta tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat
     4921 atttatcgac gacgatatag cctttttcgt tggttttaac gccagcggct tcgaggttga
     4981 tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc
     5041 gaccatcttc cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga
     5101 tggcgttggt atgcaactgt gggccttcgg cgttcatcac ttcgaccagc gtttcagaga
     5161 tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc
     5221 cgaggccgtt aatcacgcca gccagctcaa cggcgatgta acccgcgcca acaaccgcca
     5281 cgcgctctgg caaagcagga agggcgaaga agccatcgga atcaataccg tattccacgc
     5341 ctggaatatc cgggtggctc ggacgaccgc cggtggcgat cagaatatga tcggccgtga
     5401 tggtttcgcc gttaacttcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta
     5461 catcaacgtt atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg
     5521 cggtacggct ggcgatcaac gtttcccagt tgaacttgtt gatggtggta tcgaaaccat
     5581 aatcagggcc gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt
     5641 tcggcacgca gccgacgttc acgcaggtgc cgcccagctc tttggcttca atcagtgcgc
     5701 atttctggcc gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga
     5761 tggcgatgta atcatagtgt ttagtcatga catattgtcc ttatcgttga ttaccgcgat
     5821 tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct
     5881 tacggtggcg tgcccggtac ctgccggaac cagtttgctg tgcagccacg acagcacgtt
     5941 attcatctgc tgttccagct tccacggcgg gttaatcaca atcatgccgg aagcggtcat
     6001 gccacggcga tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc
     6061 ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca ccggatacca
     6121 caatgcgtaa gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc
     6181 ctgatagtcg gttttcattt catacggcgg gtcgataagg attaaaccac ggcgagaaac
     6241 cggcggcagt ttggccttaa gttgctggaa accgtcggct ttttcgacgc gtgcacggct
     6301 gtctttctga aatccagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg
     6361 caggctgtct tgttcgcgca gtagctggcg agcaatcaac ggcgaaccgg ggtagtaacg
     6421 caactggccg ctacggttga agtgttttac cacattgatg tacgcctcca gttctgcggg
     6481 caaatcgtcc tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc
     6541 atgttcgcta cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg
     6601 tttatctttc tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc
     6661 ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt
     6721 aagtaaaaat tggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa
     6781 gaggccgccg ccgcgaaagc gtaacgtttc ccattgaaat tcactacact taaccccatg
     6841 ctacacacat tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc
     6901 gctaatgacg aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc
     6961 ggaacatgtc gttccagccg tgactaaggc attgaacgac tgccgcgaaa atgtggagcg
     7021 cgtagtagcg caaggggcac cgtacacctg ggaaaatctc tgccagccgt tggcggaagt
     7081 ggacgatgtt ttggggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag
     7141 cccggaactg cgtgaagcgt acgaacaaac cctgccgctg ctgtcggaat acagcacctg
     7201 ggtagggcaa catgaagggc tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta
     7261 cgccacgctg aacacggcgc agaagaaagc ggttgataac gccctgcgtg attttgaact
     7321 ctctggcata ggtctgccga aagagaaaca gcagcgttac ggcgaaattg ctacccgtct
     7381 ttctgaactg ggcaaccagt acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa
     7441 actcgttacc gacgaagcgg ggctggcggg gatgccagaa agcgcgctgg ctgcggcaaa
     7501 agcccaggcc gaagcgaaag agctggaagg ctacctgctg acgctggata tcccaagcta
     7561 cctgccggta atgacctact gcgacaacca ggctctgcgc gaagagatgt atcgcgctta
     7621 cagcacccgc gcctccgatc aaggcccgaa cgccggtaag tgggataaca gcaaggtgat
     7681 ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg ctgggctttg aaaactacgc
     7741 ctttaaatcc cttgccacta aaatggcaga aaacccgcag caggtgctgg atttcttaac
     7801 cgatctggca aaacgcgcgc gtccgcaagg cgaaaaagag ctggagcaac tgcgtgcctt
     7861 cgctaaagcc gaatttggtg tcgatgagtt gcagccgtgg gatatcgctt actacagcga
     7921 aaaacagaaa cagcacctct acagcatcag cgacgagcag ctgcgtccgt acttcccgga
     7981 aaacaaagcg gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa
     8041 agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga
     8101 aaataacgaa ctgcgcggca gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg
     8161 cggggcgtgg atggatggct gcgtaggcca gatgcgtaaa gctgatggtt ctctgcaaaa
     8221 accggtcgcg tatttgactt gtaacttcaa ccgcccggta aatggtaaac cggcgctgtt
     8281 cactcacgac gaagtgatca ccctgttcca cgagttcggt cacggcctgc accatatgct
     8341 gacccgcatc gaaaccgctg gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga
     8401 actgccgagt cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc
     8461 tggtcactat gaaaccggcg aaccgctgcc gaaagagttg ctggataaaa tgctggcggc
     8521 gaagaactac caggcggcgc tgtttattct gcgtcagctg gagttcggcc tgtttgattt
     8581 ccgccttcat gccgagttcc gcccggatca gggggcaaaa atcctcgaaa ctctggcaga
     8641 aatcaagaaa ctggttgccg tggtgccatc tccgtcctgg ggccgtttcc cgcacgcttt
     8701 cagccatatt ttcgccggtg gttatgcggc aggttactac agctacctgt gggctgacgt
     8761 actggcggca gatgctttct cgcgctttga ggaagagggc attttcaacc gtgaaaccgg
     8821 gcagtcgttc ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt
     8881 caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg ctggagcatt acggcattaa
     8941 gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga gacggcgcct
     9001 tatctgttct ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg
     9061 tgttaacgcc ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatct
     9121 ttgttgattt tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg
     9181 aggcggtggc gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcca
     9241 ccgcagggct ggggcgcgat gcctttgtac tggcttcagt cggctgccgc gtgcggatgc
     9301 tggagcgtaa tccagtggtt gccgcgctac tcgacgacgg cctggcgcgt ggttatgcgg
     9361 atgcggaaat cggcggctgg ttgcaggagc ggttgcagtt aattcacgcc tccagcctga
     9421 cggcgctaac cgatattacc ccacgcccgc aggtggtcta tctcgatccg atgttcccgc
     9481 ataagcagaa aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc
     9541 cggatcttga tgccgatgga ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg
     9601 tggtaaagcg cccggactac gcgccaccgc tggcgaatgt cgccacgcca aacgcggttg
     9661 tgactaaagg gcatcgcttt gatatctatg caggtacgcc agtgtaaaag taaagcaggg
     9721 tagcgataac gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc
     9781 atgcgtttca gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga
     9841 ccaatcttac caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg
     9901 tccggcaccg cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc
     9961 aggaaccaca tcccgagaat aaagcccatc aaatgctgcg gcaccagggc agcaatcatc
    10021 gccaggccaa gggcgctaat aaacaattca cccaggctct agaataaata caccagcacg
    10081 ataaaccatg gcgatgtcag cccctggtca tccgcaaacc acattcccgc agcagcagtc
    10141 gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa atttcatcgg catcgagagg
    10201 tctttgcctt tgttacccag atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc
    10261 acccagaacg ggttaagcgc ctggaagctg accgggttga tggaaaaacc gagaatttca
    10321 tgatgcacgt tgttgatggc aaagaagttc agcgatgttg gcatctgggc gtagagaatg
    10381 taaaacacca ccgcttcgag catcaggaca aaggcgacaa acattttatt gcgcccggtt
    10441 ttatccagct tgaatgcctg acgaaagaag atgatggtga cgacgatgga gagaacaatc
    10501 agcaccagat tggcgacttc tacgttgtgc atcagccatg cacatacgaa gatcatcacc
    10561 acgctgccaa gtaatacgta caacagtttg ctgaagctca tcggcttgaa gtcgggttca
    10621 gaaccaatgt ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc gataattaac
    10681 cccgccccgc acaggttgta ggtgactgaa taaccgaatc tatcagcgat cacaggggcc
    10741 agcgataacg ctatcaacga gccgatattg atcgacatat agaacagggt gaatgcgcca
    10801 tcaagccgcg gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta
    10861 aacaggccgt taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt
    10921 agcgacatgc cggtcatgaa gtagccaatc gccagcacaa gtgctccaag aacaatggtg
    10981 cgtttggtcc ccaacaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc
    11041 agtgcagcaa aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc aagctgttta
    11101 acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg
    11161 acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta
    11221 ttaatattta ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc
    11281 gcgaaaaagt ttacatctgt cagagattct taatattgat gcggtgaata gattaataca
    11341 ctattacaac agaaaataac cagctgatca cactaatgat ggtgttaaat tttgtattgg
    11401 caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac gctgcatcag
    11461 gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt
    11521 ggagggatga ttattcttct tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg
    11581 tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg tggtgaccac
    11641 aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg cccaggtcgc
    11701 cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc tcggtcagcg
    11761 catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca tcaataagcc
    11821 cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtagg
    11881 ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag agatcgactg
    11941 cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg
    12001 ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc
    12061 taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat
    12121 ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa tatattgata
    12181 gtggttaacc ttctggaaaa aaaacaacct gatctcctac actatctata gagccgctcg
    12241 gatgttggtc accacaaaag cggtttctcc aggacctggt gttaactgat tggcagcgta
    12301 tcgggagcgg tagccccggg gtggaactcc gcgggcaggt cgccggggag gaggtatgat
    12361 aagcaccgtc gcattatttt gggctttatg tgtcgtttgc attgttaaca tggcgcgcta
    12421 tttctcatca ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat tgctctatca
    12481 atatgttgat ggagggggct tttttacctc acatggccaa cccaacaaac aggtgcgtct
    12541 cgtttggtat atctatgccc aacgttatcg cgatcatcac gatgatgagt ttattcgccg
    12601 ctgtgagcgg gtgcgtcggc agtttattct gactagcgca ttgtgtggtc tggtggtggt
    12661 cagcctgatt gcattgatga tttggcatta agcacttcag caataataac gcataaaaaa
    12721 agcgggccag taaactgacc cgctcgtttt ggtgcgtacg attacaggaa ctgcaaggag
    12781 aaccagtaca gcccgccgga aagtagtacc gcagccggaa gggtaaacac ccaagccatc
    12841 agaatactgg ttacggtttt acgctgtaag ccgccgccat ctaccaccat cgtccccgcg
    12901 acagaagagg agagtacgtg agtggtggaa accggcatcc cggtataact cgccaggccg
    12961 attgacactg ccgccgtcat ctgggcagac atcccctgag cgtaggtcat gcctttctta
    13021 ccgattttct caccgatagt cgttgccaca cgacgccagc caatcatcgt accgatacct
    13081 aacgccagcg cgaccgccat gatgatccac accggtgcat actcgatggt gctaagcatg
    13141 tcggacttca gtttcttcaa caggcgctga tcgtcagcac tcacaccagg catcttcacc
    13201 actttgtcga tagtgtcaga aacgcacagc ataatgcggc gcatctggct acgttgatca
    13261 agcgacagct tgtcgtagct ttccacatcg gtggtcaaca tacctttcag gcggttgagc
    13321 gcgttaatgg tattcgacgg atggcagtgg aactccgcag gttgcgttgc gccagcttcc
    13381 ggagccggta ctaactgatc agcaccggta gcctgtttga gcagcgcagg atgctgctca
    13441 aagtaagctt cgacgttgtt gatggcatca cgggtacggg tgatttcgta gccagtggca
    13501 ttcatgttca ccacgaagcc tgctggcgcg acgccaatca ataccaacat aaccagacca
    13561 atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc
    13621 agcgcgatac gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc acgctccgct
    13681 ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa
    13741 atcagaccgc cagcaaacac caggccgaca ataggggaaa cgatcagaga accgaaaata
    13801 cttaatactt tcgggatatt gagtgcatcc accactgacg tcccggtcat caacgcattg
    13861 gttaaaccaa tcccgatgat cgcgccaatc agcgtatgag agctggatgc aggtaaacca
    13921 aagtaccagg tacccaggtt ccagataatc gccgccagca acatagagaa caccatggca
    13981 aggccatgag acgatcccat attaagcagc agatccgtcg gcagcatatg cacaatggca
    14041 taggcaacac tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc cgccataacc
    14101 acggcgagct gagaacgcat cgcgcgggta tagataacgg ttgccacggc gttggctgtg
    14161 tcatggaaac cattgatggc ttcgtagaac agcacaaaag ccagtgcaag caataataac
    14221 agcccggtat gcaaatccag gccagcaaac aaatgtagca taggacgtta cgccattttg
    14281 aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt gaaatataga
    14341 ctttttttga tttgcctcct gtatggattt cactcaaaaa ataattatct tatataattc
    14401 aggcaaatac ttccttttag taatattgat gctggtgcga ccactgagga atctttacaa
    14461 ttcacgcccg ttttttctaa gaggagcgca acgtggaaag gtttgatgcc attattatag
    14521 gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg tcaggcagga cgccgggttc
    14581 tgctgatcga taatggtaaa atactagggc gcaaaatcct tatgtctggc ggtgggcgct
    14641 gcaactttac caacctttat gtcgaaccag gcgcttatct gagccagaat ccgcattttt
    14701 gtaagtctgc actcgcacgt tttacccagt gggatttcat tgatctggtc aataaacacg
    14761 gtatcgcctg gcacgagaaa acgttagggc aactcttctg cgatgactcc gcgcagcaga
    14821 ttgtcgacat gctggtggat gagtgcgaga agggcaatgt aaccttcaga ttgcgtagcg
    14881 aagtgctgag tgtggcgaag gatgaaacag gcttcacgct tgatctgaac ggcatgactg
    14941 tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc aatgccggtg ctgggcgcat
    15001 cgccgtttgg ttataagatt gccgaacaat ttggcctcaa cgtgctgccg acccgcgcgg
    15061 gtctggtgcc attcactctg cataaaccgt tgctcgaaga gttacaggtg ctggcgggcg
    15121 tggcggtgcc ttccgtgatt accactgaaa acggcaccgt tttccgtgag aacttactct
    15181 tcacccaccg cggcttatct ggaccggcgg tgttgcagat ttcaagctac tggcaaccgg
    15241 gtgaatttgt cagtatcaat ctgctaccgg atgtggacct cgaaaccttc ctgaatgagc
    15301 agcgtaacgc acatccgaat caaagcctga aaaacacact ggcggttcat ctaccgaagc
    15361 ggttggttga acgcttacag caactcgggc aaatcccgga tgtttcgcta aaacagctca
    15421 acgtgtgtga ccaacaggca ctgattagca cattgaccga ctggcgcgta caacccaacg
    15481 gcactgaagg ctatcgcact gccgaagtga cgctcggcgg cgtggacacc aacgaa
//
LOCUS       DAJTYI010000092        15472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYI010000092 DAJTYI010000000
VERSION     DAJTYI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..465
                     /gene="tuf"
                     /locus_tag="OO564_002766"
     CDS             <1..465
                     /gene="tuf"
                     /locus_tag="OO564_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6653836.1"
                     /translation="EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGILLRGIKR
                     EEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTI
                     ELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            648..842
                     /gene="bfd"
                     /locus_tag="OO564_002767"
     CDS             648..842
                     /gene="bfd"
                     /locus_tag="OO564_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6653837.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            915..1391
                     /gene="bfr"
                     /locus_tag="OO564_002768"
     CDS             915..1391
                     /gene="bfr"
                     /locus_tag="OO564_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6653838.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1388..1855)
                     /locus_tag="OO564_002769"
     CDS             complement(1388..1855)
                     /locus_tag="OO564_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6653839.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2234..2545
                     /gene="rpsJ"
                     /locus_tag="OO564_002770"
     CDS             2234..2545
                     /gene="rpsJ"
                     /locus_tag="OO564_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6653840.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2578..3207
                     /gene="rplC"
                     /locus_tag="OO564_002771"
     CDS             2578..3207
                     /gene="rplC"
                     /locus_tag="OO564_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6653841.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3218..3823
                     /gene="rplD"
                     /locus_tag="OO564_002772"
     CDS             3218..3823
                     /gene="rplD"
                     /locus_tag="OO564_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6653842.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3820..4122
                     /gene="rplW"
                     /locus_tag="OO564_002773"
     CDS             3820..4122
                     /gene="rplW"
                     /locus_tag="OO564_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6653843.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4140..4961
                     /gene="rplB"
                     /locus_tag="OO564_002774"
     CDS             4140..4961
                     /gene="rplB"
                     /locus_tag="OO564_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6653844.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4978..5256
                     /gene="rpsS"
                     /locus_tag="OO564_002775"
     CDS             4978..5256
                     /gene="rpsS"
                     /locus_tag="OO564_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6653845.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5271..5603
                     /gene="rplV"
                     /locus_tag="OO564_002776"
     CDS             5271..5603
                     /gene="rplV"
                     /locus_tag="OO564_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6653846.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5621..6322
                     /gene="rpsC"
                     /locus_tag="OO564_002777"
     CDS             5621..6322
                     /gene="rpsC"
                     /locus_tag="OO564_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6653847.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6335..6745
                     /gene="rplP"
                     /locus_tag="OO564_002778"
     CDS             6335..6745
                     /gene="rplP"
                     /locus_tag="OO564_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6653848.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6745..6936
                     /gene="rpmC"
                     /locus_tag="OO564_002779"
     CDS             6745..6936
                     /gene="rpmC"
                     /locus_tag="OO564_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6653849.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6936..7190
                     /gene="rpsQ"
                     /locus_tag="OO564_002780"
     CDS             6936..7190
                     /gene="rpsQ"
                     /locus_tag="OO564_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6653850.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7355..7726
                     /gene="rplN"
                     /locus_tag="OO564_002781"
     CDS             7355..7726
                     /gene="rplN"
                     /locus_tag="OO564_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6653851.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7737..8051
                     /gene="rplX"
                     /locus_tag="OO564_002782"
     CDS             7737..8051
                     /gene="rplX"
                     /locus_tag="OO564_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6653852.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8066..8605
                     /gene="rplE"
                     /locus_tag="OO564_002783"
     CDS             8066..8605
                     /gene="rplE"
                     /locus_tag="OO564_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6653853.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8620..8925
                     /gene="rpsN"
                     /locus_tag="OO564_002784"
     CDS             8620..8925
                     /gene="rpsN"
                     /locus_tag="OO564_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6653854.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8959..9351
                     /gene="rpsH"
                     /locus_tag="OO564_002785"
     CDS             8959..9351
                     /gene="rpsH"
                     /locus_tag="OO564_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6653855.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9364..9897
                     /gene="rplF"
                     /locus_tag="OO564_002786"
     CDS             9364..9897
                     /gene="rplF"
                     /locus_tag="OO564_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6653856.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9907..10260
                     /gene="rplR"
                     /locus_tag="OO564_002787"
     CDS             9907..10260
                     /gene="rplR"
                     /locus_tag="OO564_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6653857.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10275..10778
                     /gene="rpsE"
                     /locus_tag="OO564_002788"
     CDS             10275..10778
                     /gene="rpsE"
                     /locus_tag="OO564_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6653858.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10782..10961
                     /gene="rpmD"
                     /locus_tag="OO564_002789"
     CDS             10782..10961
                     /gene="rpmD"
                     /locus_tag="OO564_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6653859.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10965..11399
                     /gene="rplO"
                     /locus_tag="OO564_002790"
     CDS             10965..11399
                     /gene="rplO"
                     /locus_tag="OO564_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6653860.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11407..12738
                     /gene="secY"
                     /locus_tag="OO564_002791"
     CDS             11407..12738
                     /gene="secY"
                     /locus_tag="OO564_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6653861.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12770..12886
                     /gene="rpmJ"
                     /locus_tag="OO564_002792"
     CDS             12770..12886
                     /gene="rpmJ"
                     /locus_tag="OO564_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6653862.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13033..13389
                     /gene="rpsM"
                     /locus_tag="OO564_002793"
     CDS             13033..13389
                     /gene="rpsM"
                     /locus_tag="OO564_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6653863.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13406..13795
                     /gene="rpsK"
                     /locus_tag="OO564_002794"
     CDS             13406..13795
                     /gene="rpsK"
                     /locus_tag="OO564_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6653864.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13829..14449
                     /gene="rpsD"
                     /locus_tag="OO564_002795"
     CDS             13829..14449
                     /gene="rpsD"
                     /locus_tag="OO564_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6653865.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14475..15464
                     /gene="rpoA"
                     /locus_tag="OO564_002796"
     CDS             14475..15464
                     /gene="rpoA"
                     /locus_tag="OO564_002796"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6653866.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
BASE COUNT         3992 a         3630 c         4128 g         3722 t
ORIGIN      
        1 gaagaagttg aaatcgttgg tatcaaagag actcagaagt ctacctgtac tggcgttgaa
       61 atgttccgca aactgctgga cgaaggccgt gctggtgaga acgtaggtat tctgctgcgt
      121 ggtatcaaac gtgaagaaat cgaacgtggt caggtactgg ctaagccggg caccatcaag
      181 ccgcacacca agttcgaatc tgaagtgtac attctgtcca aagatgaagg cggccgtcat
      241 actccgttct tcaaaggcta ccgtccgcag ttctacttcc gtactactga cgtgactggt
      301 accatcgaac tgccggaagg cgtagagatg gtaatgccgg gcgacaacat caaaatggtt
      361 gttaccctaa tccacccgat cgcgatggac gacggtctgc gtttcgcaat ccgtgaaggc
      421 ggccgtaccg ttggcgcggg cgttgttgct aaagttctgg gctaattaca cgttaattag
      481 ttttgaattg aaaagggcgc ttcggcgccc tttttgctgc ccgtaatttg agctcgaaat
      541 atttagtcgt aattttgctc gctgaactat ggcgcaaata aaactgtgct attgtaatca
      601 taaccattct catttacact ttgtgcagaa tttgaacggg agcaatcatg tacgtttgtt
      661 tgtgtaatgg tgtaagtgac aaaaaaattc gccaggcagt ccgtcagttt catccgcaat
      721 ctttccaaca attgcgtaag tttattcctg tgggaaatca atgcggtaag tgcattcgcg
      781 ccgcgcgcga agtgatgcag gacgagttga tgcagatgcc agaatttaaa gagattgcct
      841 gaagcccacg cttatttttt gacatcccgc tagccccatc tacgctctaa tagtggaagc
      901 ggagggacta taaaatgaaa ggtgatgtta aaatcataaa ttatctcaat aaactattgg
      961 gaaatgagct tgtcgcaatc aatcagtatt ttctccatgc ccggatgttt aaaaactggg
     1021 gcctgatgcg tctcaatgac gtcgaatacc atgaatccat cgatgagatg aaacacgctg
     1081 ataaatatat cgagcgtatt ttgtttttag aaggaatccc caacttacag gatttaggca
     1141 aactggggat tggtgaagat gtcgaggaga tgctgcaatc tgacctgcga ttagaactgg
     1201 aaggcgcgaa ggatctgcgt gaggctatcg catatgccga cagcgttcat gactatgtta
     1261 gccgcgatat gatgatcgag atccttgctg atgaagaagg tcatatcgac tggctcgaaa
     1321 ctgaactgga tctcatcggt aaaatcggcc tacaaaatta cctgcaatca caaattaaag
     1381 ttaaagatta acggcctatc agcaaactat cccagcctat aacgaaaccc gcagccgcca
     1441 gaaatggccc aaaaggcagc gggtttttta atgattcttt tcctctcatg agcagaccaa
     1501 taactacggc tccgcaagcg aatgaggcag caaggaaaac cagtcgtggt aagaaggccc
     1561 atgaatgcca ggcgccaagg gcggcgagaa acttcacatc accatagcct aatccttctt
     1621 tatgtcgcag tatgcgatac ccccagtaaa tgacagcgaa tgtgccgtaa ccaacaattg
     1681 ccccccataa cgcatcggct aaaccatcag gatgacaaac ttgatagaac aaaagacctg
     1741 accagagtaa cgggcacgta aatctgtcag gtaacaagcc atgctttgca tcccagaaaa
     1801 aaagtagggc tgataaacaa gcgtacaaaa ttagaaaggg aagggtagtg tccataaaac
     1861 atcctctgta attggagtat caccatcgaa taactccgga tgctcagcaa cagagagagc
     1921 gctgatttgt taacctgatt ccggtgaaaa acgctatctt cacaacctta caaatttcgt
     1981 ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt gcgtcaggct
     2041 cagatttgcg tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca
     2101 gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta
     2161 cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg
     2221 gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg
     2281 tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt
     2341 ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca
     2401 cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat
     2461 cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt
     2521 agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg
     2581 attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct
     2641 atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct
     2701 aacgatggct accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc
     2761 aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa
     2821 ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt
     2881 gctgacgtta aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc
     2941 gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc
     3001 gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg
     3061 gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac
     3121 gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg
     3181 atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc
     3241 gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt
     3301 tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac
     3361 tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc
     3421 gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg
     3481 tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat
     3541 cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc
     3601 gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat
     3661 catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga
     3721 cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat
     3781 gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg
     3841 ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc
     3901 aacaccatcg tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg
     3961 cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt
     4021 aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg
     4081 aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa
     4141 tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta
     4201 accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg
     4261 gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg
     4321 cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc
     4381 ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac
     4441 gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg
     4501 atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg
     4561 ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt
     4621 acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa
     4681 tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata
     4741 tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc
     4801 gcggtactgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg
     4861 gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca
     4921 agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg
     4981 ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa
     5041 gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt
     5101 cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt
     5161 gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc
     5221 ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta
     5281 ttgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga
     5341 ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg
     5401 ctgtacaggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg
     5461 ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc
     5521 gcattatgcc gcgtgcaaaa ggtcgtgcag atcgtatcct gaagcgcacc agccacatca
     5581 ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc
     5641 taatggtatt cgcctgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa
     5701 agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact
     5761 ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac
     5821 cattcacact gctcgcccgg gtatcgttat cggtaagaaa ggtgaagacg tagaaaaact
     5881 gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg
     5941 taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg
     6001 cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa
     6061 aggtattaaa gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg
     6121 gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc
     6181 tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat
     6241 cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca
     6301 gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt
     6361 ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg
     6421 cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc
     6481 acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc
     6541 ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt
     6601 ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc
     6661 ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac
     6721 ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcattgaa
     6781 gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct
     6841 gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc
     6901 gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc
     6961 aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg
     7021 tgaaacaccc gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg
     7081 agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca
     7141 agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac
     7201 tctctcgata tgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt
     7261 gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg
     7321 tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa
     7381 cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca
     7441 ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg
     7501 tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt
     7561 tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa
     7621 caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag
     7681 tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg
     7741 cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac
     7801 gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg
     7861 ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag
     7921 aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc
     7981 gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa
     8041 ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt
     8101 agttaaaaaa ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga
     8161 gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa
     8221 cgcagcagca gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa
     8281 atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg
     8341 tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtatccg
     8401 tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt
     8461 ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt
     8521 ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc
     8581 ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa
     8641 gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg
     8701 aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag
     8761 ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca
     8821 ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc
     8881 gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa
     8941 tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc
     9001 cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg
     9061 gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac
     9121 accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc
     9181 attcagcgtg tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa
     9241 gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt
     9301 gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa
     9361 aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc
     9421 aacggtcagg ttattacgat caaaggtaaa aacggcgagc tgacccgtac tctcaacgat
     9481 gctgttgaag ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca
     9541 gacggttggg cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt
     9601 accgaaggct tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa
     9661 ggcaatgtga ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg
     9721 ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc tgataagcag
     9781 gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttacaaaggc
     9841 aagggtgttc gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt
     9901 aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc
     9961 caggagctgg gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag
    10021 gtaattgcac cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc
    10081 gctgaacaac tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc
    10141 gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa
    10201 tatcatggtc gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa
    10261 ggtagaggtg taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga
    10321 tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc
    10381 tgactgtagt tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag
    10441 ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc
    10501 tgaataacgg cactctacaa caccctgtta aaggtgttca cacgggttct cgcgtattca
    10561 tgcagccggc ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg
    10621 aagtcgctgg ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg
    10681 tggttcgtgc aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca
    10741 agcgtggtaa atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa
    10801 ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggct
    10861 tgggtctgcg tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta
    10921 tgatcaacgc ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc
    10981 tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct ggggcgtggt atcggttctg
    11041 gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg
    11101 tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct
    11161 tcacttctcg taaagcagcg attacagccg aagttcgtct atctgacctg gctaaagtag
    11221 aaggcggtgt tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg
    11281 agttcgcgaa agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc
    11341 gtgttactaa aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag
    11401 tagcagatgg ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag
    11461 ctgaaacgca gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt
    11521 attccgatcc ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc
    11581 accatcattg agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt
    11641 gctctgggga tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt
    11701 cacccaacgt tggcagaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag
    11761 tacacccgtt acggtactct ggtgctggca atattccagt cgatcggtat tgctaccggt
    11821 ctgccgaata tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc
    11881 accgctgttg taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatc
    11941 actgaacgag gtatcggcaa cggtatttca atcattatct tcgccggtat tgtcgcggga
    12001 ctcccgccag ccattgccca tactatcgag caagcgcgtc aaggtgacct gcacttcctc
    12061 gtgttgctgt tggttgcagt attagtattt gcagtgacgt tctttgttgt gtttgttgag
    12121 cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtctat
    12181 gctgcacaga gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc
    12241 ttcgcttcca gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact
    12301 ggttggaact ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg
    12361 ttactctatg cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac
    12421 ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg
    12481 ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc gcctgacctt ggttggtgcg
    12541 ctgtatatta cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg
    12601 ttctacttcg gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct
    12661 caagtgcaaa ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg
    12721 aaaggctacg gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg
    12781 tgcttccgtc aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg
    12841 tgtgatttgc agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt
    12901 tttcttgcaa agttgggttg agctggctag attagccagc caatcttttg tatgtctgtg
    12961 cgtttccatt tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag
    13021 taggagtgca tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta
    13081 atcgcattaa cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca
    13141 gcgggtatcg ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg
    13201 cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc
    13261 atcaagcgcc tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg
    13321 gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc
    13381 aagaaataat cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt
    13441 aagaaaacaa gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt
    13501 gactatcact gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt
    13561 ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga
    13621 cgccgtgaaa gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg
    13681 ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga
    13741 tgtgactccg atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct
    13801 cgttttccag gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct
    13861 gagccgtcgt gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa
    13921 gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta
    13981 tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca
    14041 gttccgtaac tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt
    14101 ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg
    14161 tgcagaagca cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa
    14221 catcgcttct tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa
    14281 gcagtctcgc gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacttggct
    14341 ggaagttgat gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct
    14401 gtctgcggac attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc
    14461 aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata
    14521 tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg
    14581 gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg
    14641 tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg
    14701 aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag
    14761 atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca
    14821 cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg
    14881 agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt
    14941 ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat
    15001 gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta
    15061 ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg
    15121 cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg
    15181 atgtacgtca gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc
    15241 ctgttgacga tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc
    15301 actatatcgg tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg
    15361 gtaaaaaatc tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca
    15421 tgcgcttgga aaactggcca ccggcaagca tcgctgacga gtaaccggat ca
//
LOCUS       DAJTYI010000093        15401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYI010000093 DAJTYI010000000
VERSION     DAJTYI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1131)
                     /locus_tag="OO564_002797"
     CDS             complement(join(<1..870,870..1131))
                     /locus_tag="OO564_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR6653867.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLS"
     gene            complement(1534..1779)
                     /locus_tag="OO564_002798"
     CDS             complement(1534..1779)
                     /locus_tag="OO564_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653868.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(2424..2499)
                     /locus_tag="OO564_002799"
     tRNA            complement(2424..2499)
                     /locus_tag="OO564_002799"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2464..2466),aa:Phe,seq:gaa)
     gene            complement(2605..3312)
                     /locus_tag="OO564_002800"
     CDS             complement(2605..3312)
                     /locus_tag="OO564_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6653869.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3710..5845
                     /gene="speC"
                     /locus_tag="OO564_002801"
     CDS             3710..5845
                     /gene="speC"
                     /locus_tag="OO564_002801"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6653870.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5894..7150)
                     /gene="nupG"
                     /locus_tag="OO564_002802"
     CDS             complement(5894..7150)
                     /gene="nupG"
                     /locus_tag="OO564_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6653871.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7352..8431)
                     /gene="mltC"
                     /locus_tag="OO564_002803"
     CDS             complement(7352..8431)
                     /gene="mltC"
                     /locus_tag="OO564_002803"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6653872.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8496..8771)
                     /locus_tag="OO564_002804"
     CDS             complement(8496..8771)
                     /locus_tag="OO564_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6653873.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8799..9851)
                     /gene="mutY"
                     /locus_tag="OO564_002805"
     CDS             complement(8799..9851)
                     /gene="mutY"
                     /locus_tag="OO564_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6653874.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10012..10731
                     /gene="trmB"
                     /locus_tag="OO564_002806"
     CDS             10012..10731
                     /gene="trmB"
                     /locus_tag="OO564_002806"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6653875.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10731..11057
                     /locus_tag="OO564_002807"
     CDS             10731..11057
                     /locus_tag="OO564_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6653876.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            11241..11960
                     /locus_tag="OO564_002808"
     CDS             11241..11960
                     /locus_tag="OO564_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6653877.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12136..13182
                     /gene="ansB"
                     /locus_tag="OO564_002809"
     CDS             12136..13182
                     /gene="ansB"
                     /locus_tag="OO564_002809"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6653878.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13299..14306
                     /locus_tag="OO564_002810"
     CDS             13299..14306
                     /locus_tag="OO564_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6653879.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14362..15401)
                     /locus_tag="OO564_002811"
                     /pseudo
     CDS             complement(14362..15401)
                     /locus_tag="OO564_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
BASE COUNT         3946 a         3738 c         3973 g         3744 t
ORIGIN      
        1 gactgaggcc gccacaccaa ctgtgccgcc gccaccgatt gtaatcacat tcgatataat
       61 taaacaccgt tgcccgcatt atttcccggc tgataaagtg ttctccatgg atacattcca
      121 ctttcagcga atgaaagaag ctttccacgc aggcattatc gtagcagcaa ccttttgcgc
      181 tcatacttcc acgcagatta tgccgtttca gttgcgcctg ataatctgct gaacagtact
      241 ggcctccacg gtccgtgtga acgataacgt tccggggcct cttacgccgc cacagcgcca
      301 tctgcagggc atcgcaggcc agttgcgccg tcatgcgtgg cgacattgac cagccaataa
      361 cggcacgtga ccacaggtca atgaccactg ccagatacag ccagccttca tctgtacgta
      421 agtacgtgat gtctcctggc cacttctggt tcgggccact ggcgtaaaaa tcctgctcca
      481 acagattttc tgacacaggc aggccgtgtg cgcggtagct gaccgggctg aacttccggg
      541 aggcctttgc cctcagtccc tgacggcgca ggcttgccgc cacggttttt acgttaaagg
      601 ggtaaccctg agcacgcagt tcatccgtca ggcgtggggc accgtaacgc tgttttgacc
      661 gggtaaaagc cgcgaggaca acgctgtcgc agtgttggcg gaactgctga cgcgtgctta
      721 tccttgtccg ccgctgacac cacgtatacc agccgctgcg ggccacccgg agcacgcggc
      781 acattgcttt gatgctgaac tcagcctgat gtttttcaat aaagacatac ttcatttcag
      841 gcgcttcgcg aagtatgtcg cggccttttg gaggatagcc agctcttcat cccgttctgc
      901 cagctggcgt ttgagacgtg caatctcggt agacatctcc agttcacgtt cagaagacgt
      961 ctgctgattt tgctgtttac tgcgccagtt gtagagctgt gattcataca ggctgagttc
     1021 acgggctgcg gcagtaacac cgatgcgttc agcaagcttc agggcttcac tgcgaaattc
     1081 aggcgaatgc tgtttacggg gttttttact ggttgatact gtttttgtca tgtgagtcac
     1141 ctctgactga gagtttactc acttagccgc gtgtccacta ttgctgggta agatcaggct
     1201 gaggagtccc cagaaaagaa gacaggcata gagttttgtg atctactgat tgcgctaaca
     1261 aattcaatga gatcacctct atgcctgccc gttaagtatg tcaggctttt ttccgtaatg
     1321 ctctaacttc cacgcattgg tttctgacat caaataaggc tatgactttc tctccctttt
     1381 aatctcattc tgatctaaaa ccatatctta aaaaaatgca tgggtatttt aattattttg
     1441 atgttgatct aacgcactca aggaccattt tattaccgat tatccattct atttaactgt
     1501 tgcgatataa atgtttgttt taggtgcttt aattcagtac aagtcatcaa accgtaccag
     1561 accctgactg tcagtgacac cgacaggtgg gggcggcggt gtggggtcaa actgagtgtg
     1621 tctgcctctg gcccacagag ccagctctgc cgccattgcc cgtggatgat actccagctg
     1681 cacgttgaga ccggaaactg ggttgttgtt ctcatccacg aactgcagcg ccagccagtg
     1741 catccgctgc ttaccgcagg cgacttcttt gtacttcatt ttgtgactca ggcaggtgac
     1801 tgtcgtgttg ccggttgcgt ccattttgcg gttaaaccct tttaattcaa tttgttaaat
     1861 tgctttccac cttttgcggg gcgcgactta accaaaatat tcactgttat cataatatgc
     1921 aatgggctgg aggttgcagg tcatttaggg attttctgta agccccgttt tgcagaacgt
     1981 tctatctccg attgagatcc tgtctctcgg tattaccggt agtctggcca taatccccta
     2041 tggatcatat tgatatagta ggcataacac ccaaaagttg actaaagcta aatcaatcag
     2101 agtgaaaaat gttcggtttg atgtaggggg ggactgagga ggggccaacg caggtggtat
     2161 gcgttttact tcagaccgga caaagaccgg atgtatccgt gcagtaatga atccgttagc
     2221 caggtgctca tgtggaaata gtgtctattg gggatttgaa gtttgacaga aaaggccaca
     2281 gtgaccagaa gggggctact tagaagaaat gttcaggctg gatggaagag gtaaagcagt
     2341 gacatcagca ttcaaagtga ccggatatct accagaaccc cagaaagcaa aaaaccagcc
     2401 cgtaggctgg ttcttctaag tagtggtgcc cggactcgga atcgaaccaa ggacacgggg
     2461 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     2521 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     2581 actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     2641 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     2701 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     2761 cggtgtggtc agcggtaata tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     2821 taatgggata ctaattaccg acaccgcaat acccagcgag caggcgaaga tcatcgccgt
     2881 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     2941 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     3001 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     3061 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     3121 tagcgtagct aaaaccatcg ctggaaggtt ggcgcatttc acaaccagta aaataccaat
     3181 ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt ccggtaagcg
     3241 ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt tgataaaagg
     3301 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     3361 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcctta gcgaaaggtg
     3421 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     3481 tacattctct cgcttatcgc cgtttcgcgc gaaacgtttc cctttctatg ttactgctca
     3541 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
     3601 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     3661 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     3721 gaatattgcc gccagtagtg aactggtatc ccgactttct tctcatcgcc gcgtggtggc
     3781 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     3841 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     3901 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     3961 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     4021 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctggacatca
     4081 acatggtgcg ttctttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     4141 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
     4201 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     4261 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     4321 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     4381 gattcaggcg gggacgacgc cggtctatct ggaagcttca cgtaacccgt ttggtttcat
     4441 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     4501 tgcgccagaa aaagccgacc tgccgcgccc gtttcgcctg gtgattattc agctgggaac
     4561 ctatgacggc actgtctata acgcccgtca ggtgatcgat accattgggc atctctgtga
     4621 ttacattctg tttgattccg cgtgggtcgg ttacgaacag tttatcccga tgatggcgga
     4681 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatttttg tgacccagtt
     4741 ggtgcacaaa cagcaggctg gattctcaca gacgtcgcag atccataaaa aagataacca
     4801 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     4861 tgcttctacc agccctttct atccgctgtt tgctgcactg gatgttaacg ccaaaattca
     4921 tgaaggggag agtgggcgtc ggctgtgggc tgagtgcgtt gagttgggta ttgagtcgcg
     4981 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
     5041 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     5101 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     5161 tccgtgcaag ctgttactca ctacaccagg tatcaatgcc gaaaccggcg aatatagcga
     5221 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     5281 gaagtgcgat ctcaactcca ttctgttctt attaactccg gcggaaagcc acgagaagct
     5341 ggcacaactg gtggtgatgc tggcgcaatt tgaacagcat attgaggatg actcgtcgct
     5401 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
     5461 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
     5521 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
     5581 tagcgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
     5641 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
     5701 ctggggtggg gcggttcaac gttatttcct tgcgctggaa gaaggcgtga atttgttgcc
     5761 gggattttcg ccggagttgc aaggtgtcta tagcgaaacc gatgcggatg gcatgaaacg
     5821 attgtacggt tatgtgttga agtaagaata aaaaaacggg tcaccttctg gcgacccgtt
     5881 tttctttgcg taattagtgg ctaaccgtct gtgtgcctgt cgggacacga acgtgtttat
     5941 atttgaacat cgccatgaac gcgaaggcca gaaccacgga ataaccagcg aaaatcaacc
     6001 atacggtctg ccagtcggta atgccgtttt gggtgtacat ctcaacaact ttaccgctca
     6061 cgatgccgcc gaggatacag ccgaagccgt tagtcatcat caggaacgtc ccttgtgcac
     6121 tggcgcgaat tgccgggcta acttcttttt cgacaaacac cgaaccagag atgttgaaga
     6181 agtcgaatgc gcaaccgtaa acgatcatcg acagtaccag cagtacagta ccgaacggag
     6241 tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca cgccacaata ctgatcatca
     6301 ttacgttctt aataccgtag cggcttaaga agaacgggat ggtcagaatg aacagggttt
     6361 cagagatctg cgaaatcgac atgatgattg acgcatgctg cacgatgaag ctgctggcaa
     6421 acatcggatc tttgtcgaag ctgtgcagga aggtattacc gaacatgttg gtaatctgca
     6481 gttccgcgcc cagcagcatt gagaagatga agaagattgc catacgcttg tttttaaaca
     6541 gcgcgaatgc atcgaggccc agcagggttg tccagctctg attcgcttgc tgtttagcaa
     6601 ccggaatatg cggcagagtc agggtaaaca gaaccagaat ggcggaaagt gctgcgccaa
     6661 tatacagctg catgtggctt aattcgaagc cagacaggct caccacccac attgccatga
     6721 taaagccgat ggtgccccag atacggattg gcgggaagtc agtaacgata tccatcccgg
     6781 cattttgcag gcgatagtaa gagatggtgt tgattaaccc aagcgttggc atataagcaa
     6841 acgagttaat caatatcaca aggaacatcg cttccggcgt cgtgacctgt gccgccatga
     6901 acagcgtgat agcgccaatg gtgtggcaaa tggcatatac ccatttcgca cttaaccatt
     6961 tgtcggccac aatccccagt agcgcaggca taaataccgc ggcgataccc agtgagctat
     7021 aaactgcgcc aatagaagca ccgtcaaact tcagggtaac aaacatatag gagccgaggg
     7081 tcgtcagcca acttccccac agacagaact gcagaaaaga gaggattttc agctgcagct
     7141 taagattcat gttaatttcc tcacatcgtg attcggatga gtgtttgaaa aagcacattt
     7201 gaccgtgcga gatgatggga ttctatcaac ttcgcggact ggttttttgt tacctgtggc
     7261 aaataattgc aaacattccc aatttgcaaa acgaaattgt gatgtggata acatttttgc
     7321 ccctgagcat cgtcaggggc ggttaatgga attatcggcg gcggtaggat ttttgcgcgg
     7381 tattcacttt ataaagataa cggcgagatt ccgcagaggg atggcgggtc gtcagcgtct
     7441 gataaacatc gcccggcgtc atggtgttaa taatattggc agcctgaatc ttatcattcg
     7501 aaaagactcg cagcacgctg cctgcgccgc cgttataggc ggtgatgacg gcataacgcc
     7561 gcgatgttgg gttatcaatt ccgccgagat aaacattgtt cagcatcgcc agatacgcgg
     7621 tgccggtatc aatattgctg gcaggatcaa acaagaaact gcggctcggc gtgccggatt
     7681 tcccctgcga gcggaacaca tctttcccgg cagtattttg taccacctgc attaatccca
     7741 gcgcatcgga acggctgacc gcatacgggt taaaggaaga ttcagtctgc ataattgcca
     7801 gaatcagcga ctcatcaacg ccatatttcc gtgacgcttg gcggaccatg ccgagatatt
     7861 tgtgcgcacg tttatcaagg tggttcggca ccatgttaat ggtgacgctg tagatgatac
     7921 gtagcccgtt gctgcggctt ttcagacggt ttttcagcag ataatccgcg aagttgcttg
     7981 cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac cacctgaccg taaaggaaag
     8041 gttctttcga aattgtaata tcatcaacgt cggaatagag atcgaccgaa ctcggatcgt
     8101 cacccatcag caacgttttg ataattgccc ggcgcaaatg cgcggcaggt tctgtcccgg
     8161 cgatggtttc gatagtaatc gtaccgtcat cgaagttgat gtggctgcgg gtctgatatt
     8221 gatcggtgta tttcacgtag tccttaggac cggcgatcac cacctctttg aagccccaga
     8281 tgttctcaat attgtgggca aattgcccca tcagaatatc aaaaccgttg gtatctttga
     8341 cccaggcttc gttataggta tcgccttttt tggtcgtcga acaggagatg agcaacggcg
     8401 caatcaaagc cagcgcgaga tattttttca tcattccggg tgcgtgttgt gtttgttttt
     8461 atgcaagctg ccggaggcgt gctccggcac tgtttttatt ttttatcttc cggcgtatag
     8521 ccctcgatat gcacctcttt accctcgaac aggaagttga ccatctcctg ctccagcagc
     8581 ttgcggtgct cggcattcat catgttgagt ttcttttcat taatcagcat ggtttgtttg
     8641 tgctgccact gcgcccaggc ttctttggag atctcgttat agatgcgttt tcccagctcg
     8701 ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt gcaggaaagt acaaaaaatc
     8761 gttctgctca taaatcatcc tctttatcga ctcacgcgct aaaccggcgc gccagtgcgt
     8821 aactgctgta acaaacgctc cacgggagcc gccaggccaa ctgacggcgg ttgcgctaag
     8881 ttataccaga gcgcattgcc ttcatccatg cagccggtga atgacgacac gggaagccac
     8941 ataggcacaa tatctaagtg gaaatggctg aaggtatggc gaaacgcggt cagttgcgtc
     9001 aggttatcgg cagcaatctg ccgttgtgcc agccaatgcc gcaaactttc ttcgtcggca
     9061 aacttcggga aacagtataa accgccccac aatccgctcg gcggacgctg cgccagcaat
     9121 acttcatctt cgtgctgtaa caggagaaag tagccggtgc gctccggcag catctgtttc
     9181 ggttttttgc ccggataaag cgaccagcta ttgttggtgg cggcaataca tccgttttgt
     9241 agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa tcatcgcgcc caaatccatc
     9301 atcgcctgat taaaccgttc cacgccgacc gcgggcgtca cctgctcgct caaactccat
     9361 agtttattct cgacctcttt tttcccaggc cagccgctta cagcatagca gcgcgccagc
     9421 acgcgtttga cgttaccgtc gagaatcgga aagtgcttgc ccagagaaag cgagagaatc
     9481 gcgcctgcgg tggaacgccc gacgcccggt aacggcgcga cttcttcaaa ggtttccggg
     9541 aatttaccac cgtgtaaggt cgccacttgt tgtgccgctt tatgcaggtt gcgcgcgcgg
     9601 gcgtaatagc caagcccggt ccacaagtgg agaacttcgt cgagcggcgc attggcgaga
     9661 tcggtcaccg tcgggaagtg cgccataaag cgttcaaaat aggggataac ggtcgcaacc
     9721 tgagtttgtt gcaacatcac ttctgagagc catactttgt agggcgtctt gtcaatttgc
     9781 cagggcagcg ttttccgccc gtatttatcg taccagtcca gaacctgggc tgaaaattgc
     9841 gacgcttgca tggtcaccga cttcactgtt gttgggggca agattgcagc acagcgacgg
     9901 cagggtgtaa accggaactt tccgcagcca cggccttatc atgcttgcat ccggcaacta
     9961 actttggata atgcccgttt tcagaacact ttcacaagcg actaaatctt tatgaaaaac
    10021 gacgtcattt caccggaatt tgatgaaaac ggccgcccac tgcgccgtat ccgtagtttt
    10081 gtgcgccgcc aggggcgact gaccaaaggc caggaacatg cgctggaaaa ttactggccg
    10141 gtgatgggcg ttgagttcag tgaagatatg ctggatttcc ccgcgctttt tggccgtgaa
    10201 gcgccggtga cgcttgagat tggttttggc atgggggcgt cgctggtggc aatggctaaa
    10261 gatcgccccg agcaggactt cctcggcatt gaagtgcatt caccgggcgt tggtgcgtgc
    10321 ctggcttctg cgcatgaaga aggtttaagc aacctgcgcg tgatgtgtca cgatgcggtt
    10381 gaagtgctgc ataaaatgat tcctgacaat tcattgcgca tggtgcagct ctttttccct
    10441 gacccgtggc acaaagcgcg ccataataaa cgccgtatcg ttcaggtgcc gtttgccgaa
    10501 ctggtaaaaa gcaaactgca gctggggggc gtattccata tggcgaccga ctgggaacct
    10561 tatgcggaac atatgcttga agtgatgtct tctattgacg gttataaaaa cctgtcagag
    10621 agcaatgatt acgtaccgcg tccggcatca cgtccggtga cgaaatttga acaacgtggt
    10681 catcgtcttg gtcacggagt atgggactta atgttcgaga gggtgaaata atggcaaaga
    10741 accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga attccaggaa ttaggatttt
    10801 cggtggcatg gcgattcccg gaaggtacat cggaagaaca gattgataaa atcgttgatg
    10861 actttattaa cgaggttatc gaaccgaaca aactggcctt tgacggcagc ggttatctgg
    10921 cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg caccgaagaa catcaggcga
    10981 ttgtgcgtaa gtggctggaa gagtgcaaac tggatgaggt acgcaccagc gaacttttcg
    11041 acgtttggtg ggactaagaa agcatacggg cgatgacaaa tgcaaaactg cctgatgcgc
    11101 tacgcttatc aggcctacag agatgcacga tcgagtaggc gggataaggc gtttacgccg
    11161 catccggcat ggaaaacgcg tactttgtta tcaatctggg gccagcatat gctggcctga
    11221 tttgttcttg agggaagact atgatgcgca aaatgctgct ggcggcagca ctttcagtga
    11281 cggcaatgac cgctcacgcc gactaccagt gcagcgtcac gccgcgtgac gatgtgattg
    11341 tcagcccgca aaccgtgcag gtgaagggcg aaaacggcaa tctggtgatc acgccagacg
    11401 gcaacgtgat gtataacggt aagcaatatt ccctgaatgc cgcccagcgc gagcaggcga
    11461 aggattatca ggctgaacta cgtagcacgc tgccgtggat tgatgaaggc gcgaaaagcc
    11521 gcgtcgagaa agcccgtatt gcgctggata aaattatcgt tcaggagatg ggcgacagca
    11581 gcaaaatgcg cagccgtctg accaaacttg atgcgcagct taaagagcag atgaatcgca
    11641 ttatcgaaac gcgcagcgat ggcctgacgt tccactataa agccattgat caggttcgcg
    11701 ccgaaggcca gcaactagtg aatcaggcaa tgggcggaat tttacaggac agcattaatg
    11761 aaatgggcgc gaaagcggtg ctgaaaagcg gcggtaaccc attacagaat gtgctgggaa
    11821 gcttgggcgg tctgcaatcc tcaatccaaa ccgagtggaa aaagcaggaa aaagatttcc
    11881 agcagtttgg caaagatgtt tgtagccgcg ttgtgactct ggaagatagc cgcaaagccc
    11941 tggtcgggaa tttaaaataa tcctctattt taagacggca taatactttt ttatgccgtt
    12001 taattcttcg ttttgttacc tgcctctaac tttgtagatc tccaaaatat attcacgttg
    12061 taaattgttt aacgtcaaat ttcccataca aagctaaggg ataatgcgta gcgttcacgt
    12121 aactggagga atgaaatgga gtttttcaaa aagacggcac ttgccgcact ggttatgggt
    12181 tttagtggtg cagcattggc attacccaat atcaccattt tagcaaccgg cgggaccatt
    12241 gccggtggtg gtgactccgc aaccaaatct aactacacag cgggtaaagt tggcgtagaa
    12301 aatctggtta atgcggtgcc gcaactaaaa gacattgcga acgttaaagg cgagcaggta
    12361 gtgaatatcg gctcccagga catgaacgat aatgtctggc tgacactggc gaaaaaaatt
    12421 aacgccgact gcgataaaac cgacggcttc gtcattaccc acggtaccga cacgatggaa
    12481 gaaactgctt acttcctcga cctgacggtg aaatgcgaca aaccggtggt gatggtcggc
    12541 gcaatgcgcc cgtccacgtc catgagcgca gacggtccat tcaacctgta taacgcggta
    12601 gtgaccgcag ctgataaagc ctccgctaat cgtggcgtgc tggtggtgat gaacgacacc
    12661 gtactggacg gtcgcgatgt caccaaaacc aacaccaccg acgtagcgac cttcaagtct
    12721 gttaactacg gtcctctggg atacattcac aacggtaaga ttgactacca acgtaccccg
    12781 gcacgtaagc acaccagcga tacgccattc gatgtctcta agctgaatga gctgccgaaa
    12841 gtcggcatcg tttataacta cgctaacgca tccgatcttc cggctaaagc actggtagat
    12901 gcgggctatg atggcatcgt tagcgctggt gtgggtaatg gtaacctgta taaatccgtg
    12961 ttcgacaccc tggcaaccgc cgcgaaaaac ggcactgcag tagtgcgttc ttcccgcgta
    13021 ccgacgggtg ctaccactca ggatgctgaa gtggatgatg cgaaatacgg cttcgtcgcc
    13081 tctggcacgc tgaacccgca aaaagcgcgc gtcctgctgc agctggctct gacgcaaacc
    13141 aaagatccgc agcagatcca gcagattttc aatcagtact aatcacctcg tcccggcatc
    13201 gtgccggggc tttttcactt cagacttacg tccattgcca attttcatta ccctaatgat
    13261 aatcaccgga ataaattatt ccgcgcgagg gttttcgggt gaaaaagcaa tggattgttg
    13321 gcacggcgct gcttatgttg atgactggta atgcctgggc agatggcgaa ccgccaactg
    13381 aaaatatctt aaaagatcaa ttcaaaaagc agtatcacgg cattctcaag cttgatgcca
    13441 tcagcttaaa aaatcttgat gctaagggta atcaggccac ctggtcagcg gaaggcgatg
    13501 tctcttccag tgacgatctc tatacctggg tcggtcagtt ggcagattac gaactgctcg
    13561 aacagacctg gacgaaagat aaaccggtaa aattctcggc gatgttaacc agtaaaggaa
    13621 cgcccgcgtc tggctggtcg gtgaactttt actcttttca ggcggcagcc agcgatcgtg
    13681 ggcgggtggt cgacgatatc aaaacgaata ataaatatct gatcgtgaat agcgaagatt
    13741 tcaattatcg ctttagtcag cttgaatctg cgttgaatac ccagaagaac tcgattcctg
    13801 cgctggaaaa ggaagtgaaa gcgcttgata agcaaatggt ggcggcgcag aaagcagcgg
    13861 atgcatattg gggaaaagat gcaaacggta agcaaatgac ccgcgaagaa gcctttaaaa
    13921 aaattcacca acagcgtgat gagtttaata aacagaacga tagcgaggcg ttcgcggtta
    13981 aatatgacaa agaggtttat caaccggcga ttgcggcatg tcataaacag agtgaagagt
    14041 gttatgaagt gccgattcag cagaaacgag atttcgatat caacgaacaa cggcgacaga
    14101 cttttctgca atcacaaaaa ctcagccgta aattgcagga tgactgggta acgctcgaaa
    14161 aagggcaata tccgttaacg atgaaagttt cagaaataaa cagtaaaaaa gtcgcgattt
    14221 tgatgaaaat tgacgatatc aaccaagcta atgaacgctg gaaaaaagat accgaacaac
    14281 tgcggcgcaa tggggtgatt aagtaactca tgttaagtgc ctttgccgga taatataaaa
    14341 agttatccgg caaattattt tttacattag ccctagtagc tgtggaatga acaaggatat
    14401 ttgcggaatg taagtgatca tcatcaacgc tactagcaat gcagcataaa atggcagcaa
    14461 tggtttgatt aaatgttgaa tcttcacacc cgagatagaa caaccaacaa atagcgcact
    14521 acccaccggc agtgttaaca gcccaataca aaggttggcc accatcataa taccgaaatg
    14581 tactgggtcc attcccagct cttgtgcaat cggcaagaag atcggtgtaa agattagtac
    14641 agccggtgtc atgtccataa agatgccgac aattaataag acgatattaa tcagcagcaa
    14701 tatgatcagt gggttatcag aaatccccat caatgcgtcg ctgatcatat aaggaatatc
    14761 agcattagtc attgcccagg acatgcccac ggaaaaaccg atcaacagta atacaatcga
    14821 agtggtaaca accgactcca gaatcagttt gggtaaatgg cgccatttta cttcccggta
    14881 aatcagcacc gagagaataa atgtgtagac aacggcaatt gctgacgctt cagttgcggt
    14941 aaaaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    15001 tttacaggcc tgagcaaagg ttgctctttc tgaaaggggg tagccacggc gtttagcaat
    15061 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    15121 aaacagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    15181 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    15241 atcataacct tctttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    15301 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    15361 cgaaccagga acgcggccaa ccattaccta ggcgaggtta a
//
LOCUS       DAJTYI010000094        15167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYI010000094 DAJTYI010000000
VERSION     DAJTYI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..187
                     /locus_tag="OO564_002812"
     CDS             <1..187
                     /locus_tag="OO564_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6653880.1"
                     /translation="IPDYFAWGGIALVIQLLVFAGVRLYMPALSEKIINHNTAAGMFM
                     GTAALAGGIFNAACMTW"
     gene            197..835
                     /locus_tag="OO564_002813"
     CDS             197..835
                     /locus_tag="OO564_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6653881.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            838..2001
                     /locus_tag="OO564_002814"
     CDS             838..2001
                     /locus_tag="OO564_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6653882.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2085..3709
                     /locus_tag="OO564_002815"
                     /pseudo
     CDS             2085..3709
                     /locus_tag="OO564_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3826..4101)
                     /gene="yjfN"
                     /locus_tag="OO564_002816"
     CDS             complement(3826..4101)
                     /gene="yjfN"
                     /locus_tag="OO564_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6653883.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4250..4579)
                     /gene="bsmA"
                     /locus_tag="OO564_002817"
     CDS             complement(4250..4579)
                     /gene="bsmA"
                     /locus_tag="OO564_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6653884.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4761..5510
                     /gene="yjfP"
                     /locus_tag="OO564_002818"
     CDS             4761..5510
                     /gene="yjfP"
                     /locus_tag="OO564_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6653885.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5507..6262)
                     /gene="ulaR"
                     /locus_tag="OO564_002819"
     CDS             complement(5507..6262)
                     /gene="ulaR"
                     /locus_tag="OO564_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6653886.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6370..7434)
                     /gene="ulaG"
                     /locus_tag="OO564_002820"
     CDS             complement(6370..7434)
                     /gene="ulaG"
                     /locus_tag="OO564_002820"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6653887.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7789..9186
                     /gene="ulaA"
                     /locus_tag="OO564_002821"
     CDS             7789..9186
                     /gene="ulaA"
                     /locus_tag="OO564_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6653888.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9202..9507
                     /gene="ulaB"
                     /locus_tag="OO564_002822"
     CDS             9202..9507
                     /gene="ulaB"
                     /locus_tag="OO564_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6653889.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9517..9981
                     /gene="ulaC"
                     /locus_tag="OO564_002823"
                     /pseudo
     CDS             9517..9981
                     /gene="ulaC"
                     /locus_tag="OO564_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            9995..10645
                     /gene="ulaD"
                     /locus_tag="OO564_002824"
     CDS             9995..10645
                     /gene="ulaD"
                     /locus_tag="OO564_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6653890.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10655..11491
                     /gene="ulaE"
                     /locus_tag="OO564_002825"
     CDS             10655..11491
                     /gene="ulaE"
                     /locus_tag="OO564_002825"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6653891.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11491..12177
                     /gene="ulaF"
                     /locus_tag="OO564_002826"
     CDS             11491..12177
                     /gene="ulaF"
                     /locus_tag="OO564_002826"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6653892.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12306..12581)
                     /locus_tag="OO564_002827"
     CDS             complement(12306..12581)
                     /locus_tag="OO564_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6653893.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12908..13303
                     /gene="rpsF"
                     /locus_tag="OO564_002828"
     CDS             12908..13303
                     /gene="rpsF"
                     /locus_tag="OO564_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6653894.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13310..13624
                     /gene="priB"
                     /locus_tag="OO564_002829"
     CDS             13310..13624
                     /gene="priB"
                     /locus_tag="OO564_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6653895.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13629..13856
                     /gene="rpsR"
                     /locus_tag="OO564_002830"
     CDS             13629..13856
                     /gene="rpsR"
                     /locus_tag="OO564_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6653896.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13898..14347
                     /gene="rplI"
                     /locus_tag="OO564_002831"
     CDS             13898..14347
                     /gene="rplI"
                     /locus_tag="OO564_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6653897.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14418..14981)
                     /locus_tag="OO564_002832"
                     /pseudo
     CDS             complement(14418..14981)
                     /locus_tag="OO564_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            15045..>15167
                     /locus_tag="OO564_002833"
     CDS             15045..>15167
                     /locus_tag="OO564_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6653898.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYA"
BASE COUNT         3552 a         3697 c         4080 g         3838 t
ORIGIN      
        1 tattccagac tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc
       61 tggtgtcaga ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc
      121 aggaatgttt atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac
      181 atggtaatgg acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca
      241 cggagcgatc agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta
      301 cgcacaaaaa tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg
      361 ttgtagcgat agtagcgatg tcgataacga cggcgacgga acgttttacg cgacagtgca
      421 ggattgtatt gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa
      481 agcggcattt tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta
      541 cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt
      601 gttaggccgg gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc
      661 ctcatttgct tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg
      721 ttctggcaaa aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt
      781 ttcgcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg
      841 ctgagacaca acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc
      901 gactttcata tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact
      961 ctgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt
     1021 gaggtggtgg atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg
     1081 ttgtactggg atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg
     1141 atggattttg cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg
     1201 ccgacctcgt tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc
     1261 agcggcatta ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg
     1321 cgctttagtg agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat
     1381 gaagacagga gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg
     1441 cggtttatct acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat
     1501 gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat
     1561 aacggtccgc tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc
     1621 ttgagtaata aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt
     1681 cttgcatcct ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt
     1741 aaaccgatct actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc
     1801 gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct
     1861 ctgccgcgat ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg
     1921 tgcggaatgg ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt
     1981 ccgcattaca ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc
     2041 agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc
     2101 acaccatttt taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg
     2161 cgctttgcga agcggtaacg cgtgaaggtg ctggctggga tagcaatttt ctagccagta
     2221 ttggtcagca gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc
     2281 cgcctgaact gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc
     2341 ccgcatggca cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg
     2401 aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatggca
     2461 ggttgaggct gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt
     2521 acagatgtta cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga
     2581 ttctcactta ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga
     2641 aaagcagggc ggttcagatg tcatgagcaa caccatccgt gcagagcgtt tggaagatgg
     2701 ctcttatcgg ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct
     2761 ggtgctggcg cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga
     2821 cgggcaacgc aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa
     2881 cgccagttgc gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga
     2941 aggaattcgt ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctaggtag
     3001 ccatgccatg atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt
     3061 ttttggtaat ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact
     3121 tcagcttgaa gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc
     3181 cgatgccaaa gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg
     3241 caaacgaggt atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg
     3301 cgaggagagc gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg
     3361 ttccggcaat attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata
     3421 cgacttattg tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc
     3481 ggttcgtcgt ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac
     3541 tcatcagcta ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat
     3601 ggcgcaggcg tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca
     3661 gatccagaat gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac
     3721 tgatgcgacg ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag
     3781 gcgctcgcgc cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat
     3841 cgcctgtacg cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca
     3901 tgacgcacct tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc
     3961 tgaaatattg ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact
     4021 ggcgaattct gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg
     4081 tgaggcaagt aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc
     4141 attccatcgc ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa
     4201 cgcataggga cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt
     4261 acaaaatggc ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa
     4321 cgtaataatc agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg
     4381 gagaaccccg aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc
     4441 ggcgaatctc ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg
     4501 cgctacaggc acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat
     4561 tacgtttcct gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc
     4621 gaattgttac ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct
     4681 caacattatg aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac
     4741 cagacaaaaa ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg
     4801 ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc
     4861 acggctttac ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg
     4921 gtttgcgggt gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag
     4981 cgcggcggtt aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt
     5041 tacgtgcggc aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg
     5101 cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta
     5161 ccgccagcat gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc
     5221 tgatacctga aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag
     5281 agtgggaagc gacaaaccac ctagaacaac ttggggacag acctctactg ctgtggcatg
     5341 gcctcgacga tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg
     5401 agacgggacg ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgcatta
     5461 cgcctgaggc gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa
     5521 tgctgacccc ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga
     5581 tcagcatatc gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac
     5641 tgctatcaac cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg
     5701 ctgtcagcat atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca
     5761 tccagtgccc ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac
     5821 ttttgttgta ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat
     5881 ttgccagcgg tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa
     5941 gcagaaacgc ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg
     6001 aggccgcttt agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg
     6061 gcgtccagcg cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt
     6121 tgccgctttc gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac
     6181 gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt
     6241 gatgtctttg tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat
     6301 ttgaaatagc caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata
     6361 aacgacagat tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc
     6421 gaaaccgcgc ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc
     6481 gccaacctgc cagataaacg gcttgaagcc atacttcaga cgatcttttt tcatctccca
     6541 cagcacgcgg atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg
     6601 gataaccact ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt
     6661 gtcggtaata ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc
     6721 gttaccgtgc ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc
     6781 gccaggggtt ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc
     6841 agccgctttt tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc
     6901 atgaatttca atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc
     6961 tttcggtacg ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac
     7021 gtcatcagca cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg
     7081 atcgtgagtc gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg
     7141 ggtggtgcgt aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc
     7201 ctgtttcatt aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca
     7261 aacgttggtg ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc
     7321 aaatgtgcca ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc
     7381 cgggaaagtg ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat
     7441 ttaccttcat gatagttcaa ttcgaatcaa tatgtgattg gttttgatta atcctgacac
     7501 tattttttca ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat
     7561 tttgagtgta gaaagattta atggaatgta tagttaaatt aactaaatga atttaaataa
     7621 ataatttttt cgttgtgtga atcccactct atccgtgtgg aattatttgc gggtcgcgtc
     7681 acatttaatc ataaataatc ttgttgtgat tacttttgaa aattagagtg agtgcacaac
     7741 attccgggtg tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc
     7801 tacaacatct ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt
     7861 attgtgacct gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc
     7921 acgattaaaa ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc
     7981 accttcaaac cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc
     8041 gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta
     8101 ggttacgcgg tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt
     8161 accggtattc gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt
     8221 gccgttacgt tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt
     8281 ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg
     8341 acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat
     8401 aaagttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc
     8461 tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt
     8521 ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac
     8581 tggacggtct atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact
     8641 cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc
     8701 ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac
     8761 gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg
     8821 gtcgcctgcg gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac
     8881 gccaccatcg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt
     8941 ctggtgatgg ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg
     9001 agtgcctgga tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc
     9061 ttctccatcg gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc
     9121 ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct
     9181 gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa
     9241 ggcagttcca tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac
     9301 cacacggtaa atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc
     9361 atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt
     9421 ggcgtgcgca acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa
     9481 gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg
     9541 gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc
     9601 ggcgttgacc tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg
     9661 gatgcggttg aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac
     9721 ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg
     9781 ctggagttca accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggtggtc
     9841 gatgccaaca cgcactagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa
     9901 gaaaatttcg accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac
     9961 cgcaccaacg cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc
    10021 gctggacaac cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt
    10081 cgacattatc gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga
    10141 tctgaaagcg ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg
    10201 caaaattctt tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg
    10261 tgcggatatc aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt
    10321 gcagatcgaa ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg
    10381 tattcagcag gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg
    10441 tgaagccgat atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac
    10501 cggtggcctg gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat
    10561 cgccggtcgc agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg
    10621 ttccatcgcc gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg
    10681 catctatgaa aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa
    10741 aacgttgggc ttcgattttg tcgagatgtc ggtagatgaa actgacgagc gcctgtcgcg
    10801 cctcgactgg agccgcgagc agcgtctggc gctggtgaat gcgattgttg aaaccggcgt
    10861 gcgtgtgccg tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga
    10921 cgcggtgcgg gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt
    10981 tggcattcgc gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga
    11041 aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcaca
    11101 ggtgacgctg gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct
    11161 gggttacgcg cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct
    11221 gtcggcgtgg gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc
    11281 ggtgcatgtg aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt
    11341 ggtggatttc gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct
    11401 gattgagatg tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat
    11461 ggccaaagca ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa
    11521 gccaacatgg atctgccgcg ctatgggctg gtgaccttta cttggggcaa cgtcagcgct
    11581 atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg
    11641 aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc
    11701 ccatcttccg acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc
    11761 attgtccata cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg
    11821 gcattaggca ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta
    11881 agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg
    11941 ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca cggaccgttc
    12001 gcctggggga aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg
    12061 aaaatggcgt ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg
    12121 atgaataaac acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac
    12181 acgcgctgcg gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc
    12241 aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc
    12301 tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc
    12361 atcagaatta cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga
    12421 cgtgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca
    12481 tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt
    12541 agcaaaagca cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct
    12601 gactttgtgt gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa
    12661 gttgatggtg accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga
    12721 acttcaaatc agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc
    12781 ttgagtttag cggggtatgt cagtactata acgcgtcatt tttcagccga cctttaacac
    12841 gttccttgcc tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag
    12901 caattcgatg cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt
    12961 tccgggcatg atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg
    13021 tctggaagac tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca
    13081 ctacgttctg atgaacattg aagctccgca ggaagtgatc gatgagctgg aaactacctt
    13141 ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga
    13201 agcatctccg atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa
    13261 cgaaaccgct gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg
    13321 tctggtgttg tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat
    13381 tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg
    13441 gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca
    13501 cagtataacg gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa
    13561 gaacggactg agcaaaatgg ttttgcacgc cgagcagatt gaattgatag attctggaga
    13621 ctagccatat ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg
    13681 ttcaagagat cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta
    13741 agattgtccc aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg
    13801 ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg
    13861 tcacggtcca ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa
    13921 agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa
    13981 cttcctggta ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga
    14041 agcacgtcgc gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg
    14101 cgctgagaaa atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg
    14161 taaactgttc ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt
    14221 tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca
    14281 cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc
    14341 tgaataattc gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt
    14401 ctatttttcg tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat
    14461 aagtcaggat ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc
    14521 ggcactcgct cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga
    14581 ccaattttca aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat
    14641 ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt cgatataaag atcgattgtt
    14701 ggtagttcag atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc
    14761 gtttggccgg tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg
    14821 tatttactgt tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt
    14881 aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct
    14941 tcatggttcc atccaaaaga attatcacca ttcattgtta ctgaatcgcc acgggtttaa
    15001 cagacacctc agagtcattt aagatggctt aaagagaggt gcccatgagc ggtaagcgtt
    15061 atcccgaaga gtttaaaact gaagcagtca aacaggttgt tgatcgcggt tattctgttg
    15121 ccagcgttgc aacacgtctc gatatcacca cccacagcct ttatgcc
//
LOCUS       DAJTYI010000095        15074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYI010000095 DAJTYI010000000
VERSION     DAJTYI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(314..628)
                     /gene="fliE"
                     /locus_tag="OO564_002834"
     CDS             complement(314..628)
                     /gene="fliE"
                     /locus_tag="OO564_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6653899.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            842..2500
                     /gene="fliF"
                     /locus_tag="OO564_002835"
                     /pseudo
     CDS             842..2500
                     /gene="fliF"
                     /locus_tag="OO564_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            2493..3488
                     /gene="fliG"
                     /locus_tag="OO564_002836"
     CDS             2493..3488
                     /gene="fliG"
                     /locus_tag="OO564_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6653900.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3481..4167
                     /gene="fliH"
                     /locus_tag="OO564_002837"
     CDS             3481..4167
                     /gene="fliH"
                     /locus_tag="OO564_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6653901.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4167..5540
                     /gene="fliI"
                     /locus_tag="OO564_002838"
     CDS             4167..5540
                     /gene="fliI"
                     /locus_tag="OO564_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6653902.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5559..6002
                     /gene="fliJ"
                     /locus_tag="OO564_002839"
     CDS             5559..6002
                     /gene="fliJ"
                     /locus_tag="OO564_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6653903.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5999..7126
                     /gene="fliK"
                     /locus_tag="OO564_002840"
     CDS             5999..7126
                     /gene="fliK"
                     /locus_tag="OO564_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6653904.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7231..7695
                     /gene="fliL"
                     /locus_tag="OO564_002841"
     CDS             7231..7695
                     /gene="fliL"
                     /locus_tag="OO564_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6653905.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7700..8704
                     /gene="fliM"
                     /locus_tag="OO564_002842"
     CDS             7700..8704
                     /gene="fliM"
                     /locus_tag="OO564_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6653906.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8701..9114
                     /gene="fliN"
                     /locus_tag="OO564_002843"
     CDS             8701..9114
                     /gene="fliN"
                     /locus_tag="OO564_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6653907.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9117..9482
                     /gene="fliO"
                     /locus_tag="OO564_002844"
     CDS             9117..9482
                     /gene="fliO"
                     /locus_tag="OO564_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6653908.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9482..10219
                     /gene="fliP"
                     /locus_tag="OO564_002845"
     CDS             9482..10219
                     /gene="fliP"
                     /locus_tag="OO564_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6653909.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10229..10498
                     /gene="fliQ"
                     /locus_tag="OO564_002846"
     CDS             10229..10498
                     /gene="fliQ"
                     /locus_tag="OO564_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6653910.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10507..11211
                     /locus_tag="OO564_002847"
     CDS             10507..11211
                     /locus_tag="OO564_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR6653911.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            11496..12119
                     /gene="rcsA"
                     /locus_tag="OO564_002848"
     CDS             11496..12119
                     /gene="rcsA"
                     /locus_tag="OO564_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6653912.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12163..12351)
                     /gene="dsrB"
                     /locus_tag="OO564_002849"
     CDS             complement(12163..12351)
                     /gene="dsrB"
                     /locus_tag="OO564_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6653913.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12514..12741
                     /gene="yodD"
                     /locus_tag="OO564_002850"
     CDS             12514..12741
                     /gene="yodD"
                     /locus_tag="OO564_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6653914.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13039..13854
                     /locus_tag="OO564_002851"
                     /pseudo
     CDS             13039..13854
                     /locus_tag="OO564_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(13851..>15074)
                     /gene="yedQ"
                     /locus_tag="OO564_002852"
     CDS             complement(13851..>15074)
                     /gene="yedQ"
                     /locus_tag="OO564_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6653915.1"
                     /translation="GYLLRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYG
                     YVTQPWFIGHSQRENRHRAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIP
                     VRTMQQFLRNAIDKNLDGEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHD
                     TRGGIRMDSRYVSWERLDHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLL
                     SWYVIRRMVSNMYVLQSSLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVI
                     QVDLDHFKAINDRFGHQAGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLT
                     QAAEVAERIRLKLNEKEMLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYL
                     AKQAGRNRVFASDHA"
BASE COUNT         3713 a         3898 c         4006 g         3457 t
ORIGIN      
        1 attgtactac agtttgaaca caactcaccg ttttcatcct agtgtatgtg tatccaaggg
       61 gctctggcgt ggcaagagat aaaggcagga acaataatga tcagaaggaa ccccaggcta
      121 acgaatgagt tcactcttcc tgggagcaaa gcgaagacaa aacatgttat cgcgtttacg
      181 gatcgtggat ggcagaaaac aaccaggatc aggtatccat tttaaaccag aaattgagtg
      241 agtttgcccc atccatgccc cacgctgtgg gatcggatat aattaaacaa gcatagtcaa
      301 caggttacac aacctacacc tgcatagaca tcacttcctg atacgccgcc accagcttat
      361 tacgcacctg aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat
      421 cgtttagcgc cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcgactg
      481 tttgtgtatc gcttatgcga tcgatcgcgg cgtgcagctg cccggcaaaa ctaatggtcg
      541 gttgcggcag tgattcctgc gcacgcgcac tcatcgccgt agcctgtaac tggctgataa
      601 ccccttcaat cccctgtatc gctgacattt tcgtctcccg gataatttct ggtaacaaag
      661 cctaccagta agtcaataag acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg
      721 cggatagaaa aaacgaaagc acaaataatg ggagcgtcaa tttttcgagt ttgctgaccc
      781 gagagtgagt cttgttccac tttgccaata acgccgtcca taatcagcca cgaggtgcgc
      841 gatgaatgcg actgcagccc agacaaaatc tcttgagtgg cttaatcgcc tgcgtgcgaa
      901 tccgaaaatt ccattgattg ttaccggttc cgcggcagtg gcggtcatgg tcgcactgat
      961 cctgtgggcg aaagcccccg actaccgcac attattcagc aatctttccg atcaggatgg
     1021 tggcgcaatt gtcagccaac tgacgcaaat gaatattcct taccgcttca gcgaagccag
     1081 cggcgctatt gaggttccgg cagataaagt tcacgaactg cgcctgcgcc tggcgcaaca
     1141 aggtttgccc aaaggcggcg cggtcggttt cgaactgctc gatcaggaaa agtttggtat
     1201 cagccagttc agcgaacagg tgaattatca gcgggcgctg gaaggcgagc tttctcgtac
     1261 catcgaaact atcggcccgg taaaaggggc gcgcgtacat ctggcaatgc cgaaaccgtc
     1321 tttattcgtc cgtgaacaaa aatccccttc tgcatcggtg acggtaaatc tgttacccag
     1381 ccgcgcactc gatgaagggc aaattagcgc tattgtacat ctggtttcca gcgcggttgc
     1441 tggtctgccg cctggaaacg tcacgctggt ggatcagggc gggcatctgt taacccagtc
     1501 caataccagc gggcgcgatc ttaatgacgc tcagttgaaa tatgccagcg atgtcgaagg
     1561 ccgtattcag cggcgtattg aagcgatcct gtcgcctatt gttggtaacg gtaatattca
     1621 cgcctaggtc acggcgcaac tggacttcgc cagtaaagaa caaacggaag aacagtatcg
     1681 ccctaacggt gatgaatctc atgcggcgct tcgttcacgc cagcttaatg agagcgagca
     1741 aagcggttcc ggttatccgg gcggcgtacc gggggcgttg tcgaatcaac cggcacctgc
     1801 gaataacgcg ccaatcagca cgcctccgac aaatcaaaat aaccgccagc agcaggcgag
     1861 caccaccagc aatagtgggc cgcgtagcac acagcggaat gaaaccagta actacgaagt
     1921 cgatcgcacc attcgtcata ccaaaatgaa cgtgggcgat gtgcaacgtc tgtcagtcgc
     1981 ggtagtggtg aattacaaaa ccttgccaga cggtaaacca ttgcctctca gcaacgaaca
     2041 gatgaagcaa attgaagctc tgacccgcga ggcgatgggc ttttctgaaa aacgcggtga
     2101 ttcgctcaat gttgttaact cgccgttcaa tagcagtgac gaaagcggcg gagcactgcc
     2161 gttttggcaa cagcaagtgt ttatcgatca gttgctggct gccggtcgct ggttgctggt
     2221 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
     2281 cgaggcggtg aagactgtgc agcaacaggc gcaggcccgc gaggaagtgg aagatgcggt
     2341 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
     2401 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
     2461 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
     2521 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
     2581 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
     2641 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
     2701 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
     2761 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
     2821 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
     2881 tcgccaccat tctggtgcat ctgaagcgcg cccaggccgc cgatattctg gcgttgttcg
     2941 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
     3001 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     3061 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
     3121 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
     3181 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
     3241 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
     3301 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
     3361 tcgccaaccg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
     3421 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
     3481 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
     3541 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
     3601 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
     3661 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
     3721 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
     3781 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
     3841 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
     3901 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
     3961 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
     4021 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
     4081 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
     4141 cgtctggcag cactaggagt ggtgtaatga ccacgcgcct gactcgatgg ctaaccacgc
     4201 tggataactt tgaagcaaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     4261 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     4321 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     4381 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     4441 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
     4501 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
     4561 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     4621 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
     4681 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
     4741 tggggatgat ggcacgttac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
     4801 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
     4861 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
     4921 atgccacgcg cattgccgaa gattttcgcg atcgtagtca gcatgtgttg ctgattatgg
     4981 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
     5041 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
     5101 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
     5161 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
     5221 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
     5281 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
     5341 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
     5401 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
     5461 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
     5521 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaacat ggtgcgctgg
     5581 cgaccctgaa agatctggca gaaaaagagg tagaggatgc cgctcgcctg ctgggtgaaa
     5641 tgcgtcgcgg atgtcagcag gcggaagaac agctcaaaat gctgatcgat tatcagaatg
     5701 aatatcgcaa taacctcaat agcgatatga gtgccgggat gaccagcaac cgctggatca
     5761 actatcagca gtttatccag acgctggaaa aagccattac tcagcatcgc cagcaactta
     5821 atcagtggac gcagaaagtt gacattgccc tgaacagctg gcgagaaaag aaacaacgtt
     5881 tgcaggcctg gcagacactg caggaacggc aatccacggc ggcactgctt gcagaaaacc
     5941 gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg aaacctgaat
     6001 gattcgctta gcgcccttga ttaccgccga cgttgacacc accacattgc ctggcggcaa
     6061 agccagcgat gctgcacaag attttctaac gttgttgagc gaagcattag caggcgagac
     6121 aacaactgac aaagcggccc cccagttgct ggtggcaaca gataagccca cgacaaaagg
     6181 cgagccgctg gtcagcgata ttcttgccga tgcgcaacaa gcggatttac tgatccctgt
     6241 ggatgaaaca cttcctgtca tcaacgacga acaatccaca tcaacaccgt taaccaccgc
     6301 tcagacaatg acgttggctg cggtggctga caaaaatacg acaaaagacg aaaaagcgga
     6361 tgatcttaat gaagacgtca ccgcaagtct gagcgccctt tttgcgatgt tgccgggttt
     6421 tgacaatacg cccaaagtga ctgatgcacc gtcaaccgtg ttaccggcag agaaaccaac
     6481 gctcttcaca aaactgactt ctgcgcaact cactacagca cagcctgatg acgcccccgg
     6541 cacaccagct cagccattaa caccgctggt agcagaagcc cagagtaaag cggaagtcat
     6601 cagcacgcct tcgccggtga ccgctgacgc cagcccgcta atcactccac accagacaca
     6661 gccactgccc accgtcgccg cgcctgtttt gagtgcatcg ctgggttctc acgaatggca
     6721 acaatcattg agccagcata tttcgctgtt cacccgccag gggcaacaaa gtgcagagtt
     6781 gcgtctgcac ccacaggatt taggtgaagt gcaaatttcc ctcaaagtgg atgataacca
     6841 ggcgcagatc cagatgattt caccgcatca gcacgtacgc gccgccctgg aagcagcgct
     6901 gccggtactg cgtacgcagc tggccgaaag tggcattcag ttagggcaaa gcaacattag
     6961 tggcgaaagc tttagtggtc agcagcaggc cgcttcccag caacagcaaa gccaacgcac
     7021 agcaaaccat gaacctctgg cgggggatga cgacgatacg cttccggttc ccgtctcttt
     7081 acaagggcgt gtaacaggta acagcggcgt tgatattttc gcctaacgtc agaggtagca
     7141 ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa ttagccgata
     7201 agcagtagcg acacaggaag accgcaacac atgactgatt acgcgataag caagaaaagc
     7261 aagcgatcgc tttggatccc gattctggta ttcattaccc tcgcggcctg tgccagcgca
     7321 ggttacagct actggcattc gcatcaggtt gccgctgacg acaaagcgca gcaacgcgtc
     7381 gtgccctccc cggtcttcta tgcgctggat acctttacgg tcaatttggg cgatgcggat
     7441 cgcgtgcttt atatcggcat aaccctgcga ctgaaagatg aagcaacccg ctcgcggctg
     7501 agtgagtatt tgccggaagt ccgtagtcgc ttgctgttac tgttttcacg tcaggatgct
     7561 gccgtactgg cgacagaaga aggcaagaaa aacctgattg ccgagattaa aaccacactt
     7621 tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct gtataccgct
     7681 tttattctgc gataacgaca tgggcgatag tattctttct caagctgaaa ttgatgcgct
     7741 gttgaatggt gacagcgaag tcaaagacga accgacagcc agtgttagcg gcgaaagtga
     7801 cattcgtccg tacgatccga atacccaacg acgggttgtg cgcgaacgtt tgcaggcgct
     7861 ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg gggctgttca acctgctgcg
     7921 ccgtagcccg gacattaccg tcggggccat ccgcattcag ccataccatg aatttgcccg
     7981 caacctgccg gtgccgacca acctgaacct aatccacctg aaaccgctac gcggcacagg
     8041 gctggtagtg ttctcgccga gtctggtgtt tatcgccgtg gataacctgt ttggcggcga
     8101 tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc catactgaac agcgcgtcat
     8161 caaccgtatg ttgaagctgg cgctggaagg ctatagcgac gcctggaaag cgattaatcc
     8221 gctggaagtt gagtacgtgc gttcggaaat gcaggtggaa tttaccaata tcaccacctc
     8281 gccgaacgac attgtggtta acacgccgtt ccatgtggag attggcaacc tgaccggcga
     8341 atttaatatc tgcctgccat tcagcatgat cgagccgcta cgggaattgt tggttaaccc
     8401 gccgctggaa aactcgcgta atgaagatca gaactggcgc gataacctgg tgcgccaggt
     8461 gcagcattca cagctggaac tggtggccaa ctttgccgat atctcgctac gcctgtcgca
     8521 gattttaaaa ctgaaacccg gcgacatcct gccgatagaa aaacccgatc gcatcatcgc
     8581 ccatgttgac ggcgtcccgg ttctgaccag tcagtacggc actctcaacg gtcagtatgc
     8641 gttacggata gaacatttga ttaacccgat tttaaattct ctgaacgagg aacagcccaa
     8701 atgagtgaca tgaataatcc ggccgatgac aacaacggcg caatggacga tctgtgggct
     8761 gaagcgttga gcgagcaaaa atcaaccagc gaaaaaagcg ctgccgacgc ggtgttccag
     8821 caatttggcg gtggtgatgt cagcggaacg ttgcaggata tcgacctgat tatggatatt
     8881 ccggtcaagc tgaccgtcga gctgggccgt acgcggatga ccatcaaaga gctgttgcgt
     8941 ctgacgcaag ggtcagtcgt ggcgctggac ggtctggcgg gcgaaccgct ggatattctg
     9001 aacaacggtt atttaatcgc ccaaggcgaa gtggtggttg ttgccgataa atatggcgtg
     9061 cggatcaccg atatcattac cccgtccgag cggatgcgcc gcctgagccg ttagtgatga
     9121 ataaccatgc tactgtgcaa tcttccgcgc cggtttctgc tgagccactg ctgcaggtga
     9181 gcggcgcact catcgccatt attgccctga tcctcgctgc tgcctggctg gtaaaacggc
     9241 tgggatttgc ccctaaacgc actggcgtta acggtctgaa aattagcgcc agtgcttcgc
     9301 tgggcgcgcg tgaacgggtc gtggtggtcg atgtggaaga tgcacggctg gtgctcggcg
     9361 ttaccgcagg gcaaatcaat ctgctgcata aacttccccc ttcagcacca acggaagaga
     9421 taccgcagac cgattttcag tcggtcatga aaaatttgct taagcgtggc gggagatcct
     9481 gatgcgtcgt ttgttttctg tcgcaccggt ccttctctgg ctgattatgc ccctcgcctt
     9541 cgcgcaactg ccgggtatca ccagccagcc gctacctggc ggtggacaaa gctggtcgct
     9601 cccggtgcag acgctggtgt tcatcacctc gttgacgttc attccagcga ttttactgat
     9661 gatgaccagt ttcacccgca tcattattgt ttttggttta ttgcgtaacg cgctgggaac
     9721 gccctccgcg ccacctaacc aggtattgct ggggctggca ctgtttttga ccttttttat
     9781 tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac cagccattca gcgaagagaa
     9841 aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg ctgcgtgagt ttatgctgcg
     9901 tcagacccgc gaggcagatt tagggttgtt tgccagactg gcgaataccg gcccgttgca
     9961 ggggcctgaa gctgtaccga tgcgcatttt gctcccggcc tacgtgacca gcgagttgaa
    10021 aaccgcattt cagataggct tcacgatttt catccctttt ttgattatcg acctggtgat
    10081 tgccagcgtg ttgatggcat tggggatgat gatggttccc cccgccacca ttgctctgcc
    10141 ctttaaactg atgctgtttg tactggtgga tggctggcaa ttgctggtcg gttcgctggc
    10201 gcagagcttt tacagctaga gaggcaaaat gacacctgaa tcggtcatga tgatgggaac
    10261 tgaagcgatg aaagtcgcgc tggcactggc agccccgctg ttgctggtag cgctggtcac
    10321 gggccttatc atcagtattt tgcaggcagc cacgcagatt aacgaaatga cgctgtcgtt
    10381 tattccgaaa atcatcgccg tatttatcgc catcattatt gccggaccgt ggatgctcaa
    10441 tctgttgctg gattacgtcc gcaccttgtt cactaacctg ccgtatatca tcgggtagcc
    10501 cgtacaatga tgcaggagac aagcgatcaa tggctttcct ggttaagcct gtacttctgg
    10561 ccgttactgc gcgtgctggc gctcatttct accgcgccaa ttctgagcga acgcagcgtg
    10621 ccgaaacggg taaaactggg tctggcaatg atgatcacgt tcgccattgc cccatcatta
    10681 cctgccaacg atgttcctgt tttttcgttc tttgctctgt ggctggccgt gcagcagatc
    10741 ctgatcggca ttgcgcttgg ttttaccatg caatttgcct ttgccgctgt gcgaaccgct
    10801 ggcgaaatta tcggtctgca aatggggctg tcatttgcga catttaacgg tcatttatgg
    10861 ttgatttcac tgctggtcga tacctttcac accctgccga ttggtagcga accgttgaac
    10921 agcaatgcgt ttctggcacc caccaaagca gggagtttga ttttccttaa cgggctgatg
    10981 ctggcgttac cgctcattac tctgttgctg accctgaatc tggcattagg tttactgaat
    11041 cgtatggccc cgcaattatc catttttgtt attggatttc cattaactct gactgtcggc
    11101 atctctttaa tggcggcatt aatgccgtta attgcgcctt tttgcgaaca tttattcagt
    11161 gaaattttta atttgctggc tgatattatt agtgaattac cattaatata attccgtaac
    11221 gtttatcatg ctaccctaag gattatccga aaaataatat ctgcgaacat cttccaggat
    11281 actcctgtag cgaaatattt gttttaagct cactcacata tcgcaacatt tactttaaga
    11341 caattccagg caaattatac aacactttac gggatagtaa gtccgcctga aaaattgcga
    11401 gagtggcgca ttaggtgacc catgttgttc cgtttagtca tgatgaaata ttcaggtaag
    11461 gggaattatc gttacgcatt gagtgagggt atgccatgtc aacgattatt atggatttat
    11521 gtagttacac ccgactaggt ttaaccgggt atctgttgag tagaggggtt aaaaaaagag
    11581 aaatcaacga cattgaaacc gttgatgacc ttgccatagc ttgtgattca cagcgtcctt
    11641 cagtggtgtt tattaatgag gactgtttca tccacgatgc ttctaacagt cagcgcatca
    11701 agcacatcat taatcaacat cccaatacgt tatttatcgt ttttatggca attgccaatg
    11761 ttcattttga tgaatatcta ttggtcagaa aaaatttatt gatcagttct aaatcgatta
    11821 aaccggaatc tctcgacgat atccttggcg atattctgaa aaaagagaca acgataacct
    11881 cgtttttaaa tatgccgacg ttatcattga gccgaaccga atcgagtatg ttgcgaatgt
    11941 ggatggcagg tcagggaacc attcaaatct ccgaccaaat gaatatcaaa gccaagaccg
    12001 tttcatcgca taaaggtaat attaaacgta agatcaaaac gcataataaa caggttatct
    12061 accatgtcgt ccgactgacg gataatgtga ctaatggtat ttttgtcaac atgcgctaac
    12121 acattctgac tggtggtttc ccaccagtca ggctgaataa gattactctg ctttctcaac
    12181 aaagataccg tcctgatgcc ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc
    12241 cagcgcaacc aggtacattg tgccttcact aaactcctca actgccagta ccactccagg
    12301 acgacgcgga ccgctatccg ttttgactgt tacccgatca ttcaccttca tcgttttcct
    12361 cctgtggctt tgtgccagtg tagaacaatt tcgtttttct ggcagcgcca agcgcgcgcg
    12421 agtgctgatt ttctcgacgg tctatactta agagatgcca ggcggactta acgactggcg
    12481 gcaacaacag agtaacggtt gcgaggaaag atgatgaaaa ccgcaaaagt gtacagcgat
    12541 accgcaaaac gtgaggtcag cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt
    12601 agcgaaagcg aagttcatcg cagccagaac gattctgaac acgttagcgt cgatggacgt
    12661 gaatatcata catggcgtga attggcggat gccttcgaac tggatattca tgacttcagc
    12721 gtctctgaag tgaatcgttg aatgcacaat aaaaaaatcc cgaccctgag ggtgtcggga
    12781 tgaaacttgc ttaagcaaga agcacttaaa aaattcgtta caccaggaaa tctgatgtgt
    12841 tcatcacctt atctgcaatt tttttcgctg acaagaaaat attcgcgcta tgaatgatta
    12901 acgctactta tgaatagaaa tgtgacgcgc gtcacccctt ggtggctctg gtgtcaacgg
    12961 gatcgctccc gaaaaaatca tctttgatta tttgtgctgt tttgagaatc atcctggtaa
    13021 ggggagtact gcgcgaccat gttttcaatt caacaaccac tactggtttt tagcgatctt
    13081 gatggcaccc tgctggacag tcatagttat gactggcaac cggcagcccc ctggctcagc
    13141 cgtttacacg aagccaatat tcccgtcatt ctctgtagca gtaaaacttc agcggaaatg
    13201 ctgtacttgc aaaaaatgtt ggggctacaa ggcttaccgc tgattgcaga gaatggcgca
    13261 gtgatccagc ttgctgaaca atggcaggat atagacggtt ttccacgcat catctcaggt
    13321 attagccatg gcgaaatctg ccaggtttta aatacgctac gcgagaaaga acattttaaa
    13381 ttcacgactt ttgatgatgt cgacgatgca accattgccg aatggacggg attaagccgt
    13441 agctaggcgg cgctgacgca gctgcatgag gcgtcggtaa cgctaatctg gcgcgacagt
    13501 gacgagcgta tggcacaatt tatcgctcgt ctgaacgaac tgggcttgca gtttatgcag
    13561 ggtgcgcgct tctggcacgt actggatgcc tctgccggaa aagatcaggc tgccaactgg
    13621 attatcgcga cctatcaaca attgtcaggc agacgcccaa ccacacttgg cctgggcgat
    13681 gggccaaacg atgcgccttt actggaggta atggattacg cggtgattgt gaaagggcta
    13741 aaccgtgaag gggtacatct gcatgatgag gatccggccc gcgtctggcg aacgcagcgt
    13801 gaaggaccgg aaggctggcg tgaagggctg gaccattttt tctccgcccg ttaagcgtga
    13861 tcgctcgcga atacccgatt gcgcccggcc tgttttgcga ggtaaagccg acggtcggcc
    13921 aatgactgga gttgttcaaa atcataatca ccggtttcct cgctgctact tacccccagc
    13981 gaggcactga tgcgtatcgt tgtactcttg gcgatcagca tctctttttc atttaacttc
    14041 aggcgaatac gttctgcgac ctccgcagcc tgcgtcagat tcgcgcctgg cagaatcaca
    14101 caaaactcct caccaccgac ccgcccggca acgtcctgcg cacgcaagga actgctaatt
    14161 aatccggcag catgagaaag aacacggtcg cccgcctgat gaccaaagcg gtcattaatc
    14221 gctttaaaat ggtcaagatc gacctgaatg acagaaaaag gatgttggtg cgtctgacac
    14281 aatttagcga gcggacgggc tttttcgaac agtgcgccac ggttatataa acgcgttaag
    14341 gtgtcgtgcc acgcctgcca ctgcaacgag ctttgcagaa catacatgtt gctgaccatc
    14401 cggcgaatca cataccagga aaggagtaac atggtggtaa agagcgccca cagcagggtt
    14461 aatgcaatgc tgatactgcc gaaatcgccg cgcacgcctt cgcttagcgt atggacgcgc
    14521 gccagcacac cgtcgaaatg gtccagacgt tcccagctaa catagcgact gtccatacga
    14581 atgccgcccc gcgtgtcatg ttccattgcc tgcgccagca aggccagttc acgaggatca
    14641 aaaatattcc ctgttggatg gtcaggattg gaagaggtca aaaatatcag cttactgtca
    14701 tagagctgat actcaccatc gaggttttta tcgatggcgt ttcttaaaaa ttgctgcata
    14761 gtacgcacgg gaatactcat ccccagcacg ccataccagt agttattact gtctaccgga
    14821 acactgacgg taacctgcgg ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag
    14881 cgtaccgcgc ggtgacgatt ttctcgttgc gaatggccga taaaccaggg ttgggtgaca
    14941 tagccgtaat aacgcgttgg tacattgcgc ataaacaagg tcggctgcgt cgaaacgtaa
    15001 aatccagcac gcgagacata catcgcctgt tcaaccatcg acgaggagtt gtgcgccagt
    15061 cgcagcaagt aacc
//
LOCUS       DAJTYI010000096        14793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYI010000096 DAJTYI010000000
VERSION     DAJTYI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(117..926)
                     /locus_tag="OO564_002853"
     CDS             complement(117..926)
                     /locus_tag="OO564_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653916.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIPPSFVAF
                     LMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            1033..1926
                     /locus_tag="OO564_002854"
     CDS             1033..1926
                     /locus_tag="OO564_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6653917.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2061..3281
                     /gene="mlc"
                     /locus_tag="OO564_002855"
                     /gene_synonym="dgsA"
     CDS             2061..3281
                     /gene="mlc"
                     /locus_tag="OO564_002855"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6653918.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3406..4101
                     /gene="bioD"
                     /locus_tag="OO564_002856"
     CDS             3406..4101
                     /gene="bioD"
                     /locus_tag="OO564_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6653919.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4054..5346)
                     /gene="clcB"
                     /locus_tag="OO564_002857"
     CDS             complement(4054..5346)
                     /gene="clcB"
                     /locus_tag="OO564_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6653920.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(5505..6119)
                     /gene="dmsD"
                     /locus_tag="OO564_002858"
     CDS             complement(5505..6119)
                     /gene="dmsD"
                     /locus_tag="OO564_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6653921.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6162..7016)
                     /locus_tag="OO564_002859"
     CDS             complement(6162..7016)
                     /locus_tag="OO564_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6653922.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7018..7635)
                     /gene="dmsB"
                     /locus_tag="OO564_002860"
     CDS             complement(7018..7635)
                     /gene="dmsB"
                     /locus_tag="OO564_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6653923.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(7646..9916)
                     /locus_tag="OO564_002861"
                     /pseudo
     CDS             complement(7646..9916)
                     /locus_tag="OO564_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(9925..10591)
                     /locus_tag="OO564_002862"
                     /pseudo
     CDS             complement(9925..10591)
                     /locus_tag="OO564_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            10574..12174
                     /locus_tag="OO564_002863"
                     /pseudo
     CDS             10574..12174
                     /locus_tag="OO564_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            12668..13036
                     /locus_tag="OO564_002864"
     CDS             12668..13036
                     /locus_tag="OO564_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653924.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            13330..14498
                     /locus_tag="OO564_002865"
     CDS             join(13330..13579,13579..14498)
                     /locus_tag="OO564_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6653925.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSGQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         3729 a         3760 c         3787 g         3517 t
ORIGIN      
        1 aacaacatca ccggaccgcg cccatagcgg gtggtcatgg ttccggcttt gggtgagctc
       61 catgtaccgg tcaaataagc ccgcgataat aagccaacca cggcctgact gacatgctag
      121 ggggaaagca tcaaccgata gccgatgtaa ttaaacagcg tgacgaacga ccccatcagc
      181 aaaaagcctt ctgcgaacaa taacggtaat ccccggtcac gccagtgcag acgaaagttg
      241 ataaataacg tcttagggcg cagcgaagtc gggcaaaaat ggcgtgattc agggaggatt
      301 ttccagaaca tcaacgccga ggccagcgcg aaacaaccga ttgccgccag agcaattcgc
      361 cagttgaaaa agtccgtgaa gacaccacta attaagcgtc cgctcatgcc gccaattgag
      421 ttgccgctga tatacaaccc catcaaaaag gccacgaaac tgggggggat ttcctcgcta
      481 agataagtca tgccaacagc tgccacgcca cttaacgaaa gcccaatcaa ggcgcgcata
      541 atcaaaatgc cgtgccagct ggtcatcatt gtcgaaagta acgtacaaat ggaggccaac
      601 agtagcgccg tgaccatcac tggtttgcga ccaatggcat cggatagcgg gccagtaaat
      661 agcaaaccga tagccaacat cgccgtggaa atggacagtg aaatactact gttcgcgggc
      721 gttaagccaa actcctgcga aagcaccgga aggataggct gcacacaata gagaagtgca
      781 aaggttgcca gtccggcaga gaacagcgcc agggtgacgc gcataaattg cggcgtaccg
      841 cgtttaataa attgatttgg ctgagaaatg ctttgcttgt cagtgtcgct tgccggagcg
      901 ccatcaacag ttgtagtacg gctcacttga aatccttgct aaatatgcct gtggatcagg
      961 cttatacata gggtaggaaa atcgaattgt tctgtctaat atattaataa tctcaaataa
     1021 gatgttttaa atatgaatat tgaacttcgt catctgcgtt actttgttgc tgttgcggaa
     1081 gagctgcatt ttgggcgcgc ggctgcccgc ctgaatattt cgcaaccgcc gctaagtcag
     1141 cagattcagg cactggagca acaaatcggt gcccgactgc tggcacgaac taatcgcagt
     1201 gtattgctga cggcagcagg aaaacagttt cttgcagata gtcggcaaat cctgtctatg
     1261 gtggatgacg ctgccgctcg tgctgaaaga ctgcatcagg gtgaagcggg ggagttgcgt
     1321 attggtttta cttcgtccgc tccttttatt cgggcggtgt ccgatacgtt atcgctgttt
     1381 cgccgtgatt atcctgatgt ccatttacaa acccgcgaaa tgaacactcg cgagcaaatc
     1441 gctccgctca ttgaaggaac gctggatatg ggattgctgc gtaacacagc gttaccggag
     1501 acgcttgaac acgcagtcat cgtccatgaa ccgcttatgg cgatgatccc gcacgatcat
     1561 cccctggcaa ataatccgag tgtaacactg gctgaactgg cgaaagaacc ctttgtcttt
     1621 tttgatccgc acgtcgggac tgggctgtat gacgatattc tcggactgat gcgacgttac
     1681 catttgacgc ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt
     1741 tccgccggtc tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaatgaa
     1801 atgcgctggg tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg
     1861 aaacatcatg aacaaagtcc agctgcgcgt aactttcgta ttcatctgct gaatgctctc
     1921 aggtgaggga aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt
     1981 aaaaatttga cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc
     2041 gaaaatatag ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa
     2101 agcagaccaa cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta
     2161 tcgatctttc ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga
     2221 tgctcgaagc acacctggtg cgagagctgg aaatcaaaga agcggggaac cgtggccgtc
     2281 cggcggtggg gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc
     2341 gcggggagat tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc
     2401 aggaattggc gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc
     2461 agttttttat ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc
     2521 cgggaattat tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa
     2581 aagagatgcc gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc
     2641 atgatatcag cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg
     2701 atgtgattca ggtggttatc gatcacaacg tgggggtggg cgtcattacc gatggtcatc
     2761 tgctacacgc aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg
     2821 gtaaacgctg ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta
     2881 ttcttgagct ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac
     2941 cgttaaccgt ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca
     3001 tcattatcgg ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta
     3061 acccacaaaa aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg
     3121 tcatctcaga cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg
     3181 agagtactca gttttctaac cagggcacga tggcaggcgc tgcactggta aaagacgcga
     3241 tgtataacgg ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac
     3301 caaaatttgc gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat
     3361 tattttccta gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc
     3421 tttattaccg gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa
     3481 gcgttagcct cccagggaaa aacggttgcg ggatataaac ccgtagcgaa ggggagcaaa
     3541 gagacacccg aagggctgcg taataaagat gccttggtgt tgcagagtgt ttcaaccatc
     3601 gaactgcctt atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg
     3661 cacagttgcc caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa
     3721 gtcgatcatg tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt
     3781 ccactctctg aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag
     3841 gaaggttgca ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg
     3901 ctcattggct gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt
     3961 gatgtgctgg gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc
     4021 gccgaacagc gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg
     4081 gtagatagag tcacggtgta acgtccgcga aattaccgac gcaattacgc aggcaatcaa
     4141 taaaccgggg agtagctgat actccccggt catttcacat atcatcaacg tcgacataat
     4201 cggcgcgtgc gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt
     4261 aatttcttcg ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc
     4321 aagaccgata aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc
     4381 cagcacggca cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc
     4441 ggttaaaaag gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga
     4501 aagcagaccc acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact
     4561 cacaaatcca cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc
     4621 cagcacacct gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg
     4681 tacgttgtag agtaacgcgt cgctatgatt aatcagattg ctaaccagca atgccacgac
     4741 ggcggaaata atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc
     4801 tataaataaa ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc
     4861 acaggcgatc cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc
     4921 ggcaagggca gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac
     4981 taccagcagc gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt
     5041 ttgcaacgct tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca
     5101 gcccatcagc aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca
     5161 gggggaaagg tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca
     5221 ctccagtagc agcatcgcat gacgaaaccc ggcaacggca aagacagcga gaataccgac
     5281 gactgttgcg ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg
     5341 atgcatgagc agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa
     5401 ccgcaaaagc agtgcgtgct ttaagagata aaaaaacgtg acattgtaac gtattattgc
     5461 cgaatcgcgc gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt
     5521 ttaaccgcga caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt
     5581 tcacccagtg cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt
     5641 gttgaccacg gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc
     5701 tccgccaacc acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc
     5761 ttcatttcaa actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg
     5821 ccaaacagca cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc
     5881 cacgggccga caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga
     5941 aaagccgtca ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg
     6001 tcactggtga gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac
     6061 agcgctccca acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt
     6121 cctaacatcc tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt
     6181 cctactgtca tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg
     6241 aggattagcc ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa
     6301 agccagcatc caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa
     6361 tctggtacca gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct
     6421 tgcagcacaa tcacctcggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt
     6481 gtggtattaa atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc
     6541 aacaataccg tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg
     6601 tcgatttgat acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca
     6661 agtaaccaga gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat
     6721 agtccgccaa cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct
     6781 ccgattcgat tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca
     6841 ataaacccga tgcccatcaa cagccagagg aaaaacatgc cacgaacaat gtgctgacgg
     6901 tctgcgtcat ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg
     6961 cattgaccca atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta
     7021 cacttcctcc ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt
     7081 aggtttgata acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc
     7141 cagagtaccg tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg
     7201 gcaggattcg acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca
     7261 cttcgtcatg tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg
     7321 gcagtagcgg cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt
     7381 atgcattgcg ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga
     7441 gatggagaga taataagcga acacgttctg gtgccagatg ccattatctt cctgccagtc
     7501 gccgccagca tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc
     7561 tttgcacgcc agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc
     7621 atattgtgtg gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg
     7681 acgggtttcc ctttgctagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc
     7741 cgtgatcgac ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg
     7801 taacgccagg cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt
     7861 taaatacccg cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga
     7921 tccacacctc ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg
     7981 tacgcgcttt gtagtggaag cccgttaact gcagtggata ggttttccgc aggggagcgt
     8041 cccagccatc aaaacctgag gtatacgcag gaatgggatg aataatttca tcttttttca
     8101 attcccaggt atctgcaatc ttcgccagtc gttcagaata aatttcgatt ttccccgaag
     8161 gtgttttcaa cgggtttgcc tgtggatctt cacggaatgc gcggaaagcg acgtagtgtt
     8221 cttccgggca ttttttctta aagatcccgg tcgttttcat ctcctcgtag tcgggcatct
     8281 cagggttacg ttccttcgtt ttcgcatgga gatatttgat ccattcatgc tgactgcgac
     8341 cttcagtaaa ggtttgataa acgtctggtc ctaagcgttt ggcgacttca ctcagcatcc
     8401 agtagatggg tttgcgttca aattttgctg aggttgcggg ttgggcgagg atcacatagc
     8461 ccatattccc tgcagattca tgagagataa ggtcttcttg ctctgttggc atcaggtcag
     8521 gcaacaggat atcgcaatac ttagccgagg ccgtcatgaa gtggtcaatg ccaacaatca
     8581 tctcgcactt gctgtcatcc tgaagcacct catgggtgtg attgatgtcg ccatgttgat
     8641 tgatcaatgt gttactggcg tagcaccata aaaacttgat ggggacatcc agtttttctt
     8701 ttccacgaac accatcacgg gtcgcggtca ttttcgtacc atggtcgatg gcatctgtcc
     8761 atgtaaaaac ggaaatctgc gttttaacag gattctcgag catcgggaac cattctaccc
     8821 ccagatccca gctacctttg cgtacacctg agttgccgcc gtttatgccg acgttaccgg
     8881 taagaacgga aagcatggca atagcgcggg acgtttgttc gccgttggag tgtcgttgtg
     8941 gcccccaacc ctgacaaata taagcaggtt ttgctgaacc gatctctcgt gccaactgga
     9001 taattttttc tgccgggatg ctggtgattt ttgctgccca ttccggcgtt ttagctatgc
     9061 cgtcaggccc ttcgcccaga atataggctt tataatgcgc gttacgtggt gcgttggcgg
     9121 gcagcgtttt ttcatcgtaa ccaacacaat atttgtcgag aaatggctga tcgaccatgt
     9181 tttcagtaat cagtacccag gcaatcgcac aggccagtgc gccatcggtg ccagggcgaa
     9241 tgggcagcca ttcatcttca cgccctgcag ctgtgtcgtt atagcgtgga tcgatgacga
     9301 tcatgcgtgc gtttgaacgt tcgagggctt gctcgacgta gtaagtgaca ccaccgccgc
     9361 tcatccgcgt ttctgccggg ttatttccga acataacgac cagtttcgta ttggcgatat
     9421 catccgggct gttgccatca ttggcaccga acatataact cattgcggca ctgatctgtg
     9481 cggtactgta gctgccatag cgactgagaa aacccccgca agagttcatc agacggtacg
     9541 ggacgttcga gttggtgata tttccgccat ctacgcctgt tccgtacagg acatgtacag
     9601 cctcattgcc gtaatctttc aggatccgcc gaagattatc actgatggta tccagggctt
     9661 cgtcccaact tatccgttca aatttacctt caccgcgctc gccgacgcgc ttcatgggat
     9721 atttcaacct gtcaggatga ttcatccgtc ggcggataga gcgcccgcgt aaacatgccc
     9781 gaacctgatg attaccgtaa acgtcgtcac ctgtcgtatc agactccacc cagtacacgg
     9841 tgtcatcttt cacatgcaaa cgtagcagac agcggctccc gcagttaacg gtgcaggaac
     9901 tccagaccgt tttctcttct acccatcttt ttccagttcc caggtacggg caatttccgc
     9961 cagcttgctg gaataaattt caatcttacc ggaaggcgtt ttaagtggat ttgcctcggg
    10021 gtcgtcacga aatgctttgt aggcgacaaa atggccattg ggatctttac gcttatagat
    10081 acccattttt ttcagttcgt cgtaagacgg taacgccgga tctttggcaa gcattttggc
    10141 gtacagatgt tgtaaccatt gttcctgcgt gcgaccttct gtgaactttt gatagacgtc
    10201 aggtccaaga cgtttcgcga cttcactcag gatccagtaa atcggtttac gttcgaattt
    10261 ttcgctggtg actggctgga ggaaaatgag atatcccatg ttaccggcgt agtcgttagg
    10321 aataatatct tcctgctcaa cggtcatcag gtctgccagc agaatgtcgg catattttgc
    10381 cgtatgaggt cataaagttt tcgatgacca caatcatttc gcatttcgat tcgtcctgca
    10441 gaatttcatg cgttttgttg atgtcagaat gctgattaac gagggtattt cccgcgtagt
    10501 tccagatgga ctccactgtc agacggatgg atggacttga agtagttgac tcaacgctac
    10561 ttaaggtcct gatatgactg acattaacca tgataatact gtatatgcat ccagttcttt
    10621 ctctgggtct ccctatctgt atcagcaagt ctacacctca agtcctgaca caaggctgac
    10681 acaaatcaag gaccttgcag tcccgcactt gctcgcaagg ctcgctacgc cacagttagt
    10741 ccgtagagct aacggtcgat atactatccg cttcagactc aagggacaga ccacaccgtt
    10801 tctttcagta tcgacccgca gcacagacag gagagtagcc acaatgcgtc agagagagct
    10861 tgcagccacc gctaaggcct tcatgttgga taggcctgaa gtctcactac aggagcttac
    10921 agagcatctc cggtctatgg ctgagcagtt cctgacagac gctagtgacg attactggaa
    10981 tggacttgag gtcgctactt tggtggacga gaagtccaac ctgagggagc tagccgctac
    11041 gcaagccttg agtctggacc agcagaaggg cataaggtta gcgcttgaag tcctgaccgc
    11101 agcacagcag agggtagaca ctggtgatac ctctggtctg attaagctga ttgatgataa
    11161 taaccatact gattactcaa ctataggcga ctctacgtca attctgaaga atgagcaggg
    11221 ggatagccag cagttttcac acaggagcgc caaccatctg tagttttttc aagcctagtg
    11281 tcctcgctgc tggctgagaa ggcccagacc ctgaagtcat ccagctacaa ggacctgtcg
    11341 tcctcactga ataccgtctc tcggttctta cctgaagaca tggacctgat gtcccgctct
    11401 gagtggctgg ctgtcaggga cgctatgctg gcttctgagg tgagaccgtc gacgattaac
    11461 aagctgctga ctaaggcgaa gatgtgtctt gattacggtc tgatgaatgg gcaacttgag
    11521 ggccgcaacc ctattgaacg gatgaagctg accaaggata tcgactccaa gcgcagagcg
    11581 ttcacagacg aggagctgga gagacttctg gtccgtgttg aggctgagta tcagttcaca
    11641 cgacacacgg ctcacacgac ctctgaggcc cgtagatggg cgtctctggt ttctgtagtt
    11701 actggcgctc ggtctgctga ggtctgccac ctgactaaac gtgacattgt aaccttagac
    11761 aatggactgg tctgtatcga tataaacgaa gatggggacg gtaagtctgt gaagaacaag
    11821 cattcagtcc gacttgtgcc gctgaccgat ggcgttcatg ggttcgacct tgagtctttc
    11881 ctcaaatggg tagacatgca gcctgatgat ggtcctctct tcgggatgac tccgagcgcc
    11941 tactctagct ggtttaactc aagggtcctg actgaggccc taggagactc tcaggatgtc
    12001 tctctccaca gtctgagaca ttggctggcg acccgaatga aagagcgtgg agtcaatctg
    12061 gtggacgctc aaggcatctt aggtcacagc agtcagtcaa ttacgtatga cctgtacggg
    12121 aaaggacacg ctgtgggtag gctggctgat gtgcttaaga cagctttact ttaagtaggg
    12181 agacccaagg tggagaccat atacggcttg atggttgaga ccttgggtca ctgaagtctg
    12241 actgaagtta gggagggtga taataatcat aatccctcct gactataggc ccactggtcc
    12301 gccttcggct gtccgactct aagtctgtct gtggtcttat ttaaactgcg tcctaaactc
    12361 cagtccctcc aaattcctat ggtctcctct tgacattcct gtttaactgt gagtcgcctc
    12421 tggtctctct ttatatatac ttccttaatt cctgaggcgt ctttagtcct tgacatttgg
    12481 ttcaacctgt gagtcacgtc cggtccccct ctttatattt acttccttaa ttcctagggt
    12541 cgccttaatc cgtgacatcc agtccagcct gtgagtcaca tcctgtcgcg ctactgtatg
    12601 catcaccagt gatggacttg attatcacta taggggaacc aactaattca actacaggag
    12661 atactctatg gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca
    12721 gcagcactgc ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga
    12781 tgatgtcctt actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt
    12841 cagcccatac ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt
    12901 gcaggagtcc ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta
    12961 cgctcgctac gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag
    13021 taaagctggt tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct
    13081 ctgagtctga tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct
    13141 aagtaatcaa ggcatgtgac cgcctctgtc agacctcgtg tatcgacctc cagctcgtta
    13201 gcgttcttca gtcatctcag ttagtagctc gtctactcac tacattggct taagtcttga
    13261 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaacaca
    13321 ggtgctccaa tgaaaaaaag aaattttagc gcagagttta aacgcgaatc cgctcaactg
    13381 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
    13441 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
    13501 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
    13561 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
    13621 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
    13681 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
    13741 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
    13801 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
    13861 ctggcaggct catgaaagag ctggggctgg tcagcggtca gcagccgact caccggtata
    13921 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
    13981 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
    14041 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
    14101 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
    14161 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
    14221 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
    14281 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
    14341 gttacgtaag cttcagcgag gcagctcacg ccataacgga ctatatcgtt ggatattaca
    14401 gcgcactaag accgcacgaa tataacggag ggttaccccc aaacgaatcg gaaaatcgat
    14461 actggaaaaa ctctaactcg gtggccagtt tttgttgacc acttcatcta tcaccgctca
    14521 ccatctgggg tccttacagc tcgtcctgac atgcggtctc gccttatatg cacatggaga
    14581 acatggtcac tccagaccaa cactaatcaa cattaaccct attcactttc gttaagcact
    14641 ttttggtggg tccgtttgga tgcattgaat aggtgcggac tccacatgga gacagaaagt
    14701 gctttaccaa aggagcatcc tttatgaata ccaacgaagt tagcgtcatc aaccttgacc
    14761 agcttgtgtc tatgagtagc tacgatttct tga
//
LOCUS       DAJTYI010000097        14566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYI010000097 DAJTYI010000000
VERSION     DAJTYI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..420
                     /locus_tag="OO564_002866"
     CDS             <1..420
                     /locus_tag="OO564_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653926.1"
                     /translation="IYDLLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(498..1409)
                     /gene="rdgC"
                     /locus_tag="OO564_002867"
     CDS             complement(498..1409)
                     /gene="rdgC"
                     /locus_tag="OO564_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6653927.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1534..2442
                     /gene="mak"
                     /locus_tag="OO564_002868"
     CDS             1534..2442
                     /gene="mak"
                     /locus_tag="OO564_002868"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6653928.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2585..3766)
                     /gene="araJ"
                     /locus_tag="OO564_002869"
     CDS             complement(2585..3766)
                     /gene="araJ"
                     /locus_tag="OO564_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6653929.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(3892..7035)
                     /gene="sbcC"
                     /locus_tag="OO564_002870"
     CDS             complement(3892..7035)
                     /gene="sbcC"
                     /locus_tag="OO564_002870"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6653930.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7032..8234)
                     /gene="sbcD"
                     /locus_tag="OO564_002871"
     CDS             complement(7032..8234)
                     /gene="sbcD"
                     /locus_tag="OO564_002871"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6653931.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8424..9113
                     /gene="phoB"
                     /locus_tag="OO564_002872"
     CDS             8424..9113
                     /gene="phoB"
                     /locus_tag="OO564_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6653932.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9171..10466
                     /gene="phoR"
                     /locus_tag="OO564_002873"
     CDS             9171..10466
                     /gene="phoR"
                     /locus_tag="OO564_002873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6653933.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10873..12192
                     /gene="brnQ"
                     /locus_tag="OO564_002874"
     CDS             10873..12192
                     /gene="brnQ"
                     /locus_tag="OO564_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6653934.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12268..13641
                     /gene="proY"
                     /locus_tag="OO564_002875"
     CDS             12268..13641
                     /gene="proY"
                     /locus_tag="OO564_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6653935.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            13797..>14566
                     /locus_tag="OO564_002876"
     CDS             13797..>14566
                     /locus_tag="OO564_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCR6653936.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDS"
BASE COUNT         3207 a         3716 c         3923 g         3720 t
ORIGIN      
        1 atctatgact tactgacgcc aacgatcaac ctgcaaattc actgtacccg ttatcccatg
       61 cgcagccaaa atggtggaag taccgattta ccgcgttatt ggatcacgtt ggataaaaac
      121 gtgatctggg attacccgaa agactttatt gcaggtaacg ctggagtgcg taattttcac
      181 ggtgaaactt gctggtatcc ttacctgacg gatatttgtt caatttctga tttattgcgt
      241 gaatatattg ataccccaaa agcggagctg ttaacaaaac aatttaccag cgataaatgg
      301 gggttagtga atattctgcg tgctgctgac aggcgaatag ggatgaggcg attagatcag
      361 ttacggcgta aaacgcataa tattgctgcg ctaaaaatta ttgctcgccg tagcgaataa
      421 ttatatgcct ggtgtggctt cgtacgccgg ataagacgcg gcaggcgtcg catccggcat
      481 taaaggaaaa tcagcaatta acgttgtgct tcgccaccta atccttcaat caggttttga
      541 attaacgctg ccagttcacc agtcatcagg atgaaatcgg catcaaaacg ctgggcgaaa
      601 tcttcacggt cgatatcttc gttttgatcg tgcagctcgt cgcagaactt aagacgcttg
      661 agcgaaccat cgtcgcacat cacaaactga atgcgctgct gccagtcgag cgccagttta
      721 gtcaccactt ttccggcttc aatgtgattg gtgatctctt cgctggtcag atcttgtttc
      781 ttcgcgcgga tcacgccgcc atcttccagc aacgatttca gctcggcttc atcaagcagc
      841 tggaagccct gtgccgcact accggagcga acccattcgg tcagcgtcag ttcaatcggg
      901 ttttccatgc tcaatggtac aaccggtaac gaccccaggc ttttacgcag taatgccagc
      961 gtatcttccg cttttttggc actggcgcag tccaccataa tcaaaccgtt aaccgtgtcg
     1021 agccacatca ttgtctggct aaaacggctg aaagcacgcg gcagcagaga gtgcagcact
     1081 tcgtctttca gcgaatcttt ttcggttttc ttcagcttac gcgcctgttc cgcttccagt
     1141 ttggcgattt tcgcttccag cgcctgttta atcaccggag acgggaggat tttttcttct
     1201 ttgcgcgcgc agataacaat ttgaccattg gcaacgtgcg ttaacgcatc gctgtgcgat
     1261 cccatcggag gaacccagcc catcttcgcc atgtcctggc tgccgcatgg ggtaaatgcc
     1321 atcgaggcta gctgtttttc catctcttct gcacgcagcg aaatctcgcg gctaagacgg
     1381 taaaccatta aatttttgaa ccacagcatg ataatttcca cggccttgtc gttaaattta
     1441 gcgggcatga taacgaattg tcggcggcct tgcattgcca atccggttgt ccgtctctac
     1501 gctattgata ttgaaaaaaa taaggagagt acagtgcgta taggtatcga tttaggcggc
     1561 accaaaactg aagtgattgc actgggcgat gcaggggagc agttgtaccg ccatcgtctg
     1621 cccacgccgc gtgatgatta ccggcagact attgaaacga tcgccacgtt ggttgatatg
     1681 gcggagcagg cgacagggca gcgcggaacg gtaggtatgg gcattccagg ttcgatttcg
     1741 ccctacaccg gtgtggtgaa gaatgccaat tcaacctggc tcaacggtca gccattcgat
     1801 aaagacttaa gcgcgaggtt gcagcgggaa gtgcggctgg caaatgatgc caactgtctg
     1861 gcggtttcag aagcagtaga tggcgcggca gcgggagcgc agacggtatt tgccgtgatt
     1921 atcggcacgg gatgcggcgc gggcgtggca ttcaatgggc ggacgcatat cggcggcaat
     1981 ggcacggcag gtgagtgggg acacaatccg ctaccgtgga tggacgaaga cgaactgcgt
     2041 tatcgcgagg aagtcccttg ttattgcggt aaacaaggtt gtattgaaac ctttatttcg
     2101 ggcacgggat tcgcgacgga ttatcgtcgt ttgagcggac atgcgctgaa aggcagtgaa
     2161 attatccgcc tggttgaaga aagcgatccg gtagcggaac tggcattgcg tcgctacgag
     2221 ctgcggctgg caaaatcgct ggcacatgtc gtgaatattc tcgatccgga tgtgattgtc
     2281 ctggggggcg ggatgagcaa tgtagaccgt ttatatcaaa cggttgggca gttgattaaa
     2341 caatttgtct tcggcggcga atgtgaaacg ccggtgagta aggcgaagca cggtgattcc
     2401 agcggcgtac gcggcgctgc gtggttatgg ccacaagagt aaaaaacgta ggcaattggc
     2461 gcatcatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaaaatg tgccagaacc
     2521 gcgtagggcg gataaggcgt tcacgccgca tccggcaata agcaacgcgc accgtgacag
     2581 taatctaccc cagtggtttc gccagcaccg gactatccgc cgcttgctgg cgcttatagc
     2641 gaccatacag cagcaacgat gacatcgcag caaacgaaaa cagggcggca ggcagtgcca
     2701 cgtaattata tgccagcccc agcgtcagca tcatacctcc gcaatatgcg ccgacggcgc
     2761 taccgaggtt aaacgctatt tgcccacctg cggcacctaa taactctccg cctttggcat
     2821 tttgcagtaa caatatttgc agaggtgctg aaagggcaaa taatcccgcg caacaaataa
     2881 aagcaaaaat aagcgacgtt gttttcatgc cgccgcagaa aaagagcatc agcagtgcca
     2941 gtacaatgat aaagtcagtc actgctgcaa tgcgcagtgg tgaataacgt cctgaaatcc
     3001 tgccacttag catatttccc agcaccatcc ctagcccaac taacatcata ataaaggtca
     3061 tcgccgtttc cgaaaaaccg gaaataaaca tcatgtatgg ctttacgtag ctgaaccagg
     3121 caaacacacc tgcgttgcca aacatcgtgg cggcgaaaat taaccacggg gccgggctgc
     3181 gcaaaaagtg aaattgttcg cgcagatttc ctttcgcctc gtcgcgaata tctggcaccc
     3241 aaaaatagac cgatgccatc accgcaatat taaaaacagc gatcaataaa aaggtgtaac
     3301 gccagcattc ctgacttaaa tacgttccca gcggaatgcc cagcaaattg gcgactgtca
     3361 tcccggaaac catccccgcc acggcggcgg tgacttttcc gggtttgata atttttgata
     3421 acacgatcgc tccgacgcca aaaaatgcgc catgcggaaa gccggatacc agccgaccaa
     3481 tggcgagcat caggtaagac gaagagagcg tgaacatggc gttgccaatg acgcacaacg
     3541 ccaccagaaa caacaagata tgtttgagtg agtagcggct ggaaaagagt gcgatgattg
     3601 gcgcaccgac caccaccacc agtgcataat acgagatcat atgcccggca gcaggaatcg
     3661 aaattcctac gttatgcgcc agctccgtga gcacgctcat aatgccaaat tcggctatcc
     3721 ccaaaccaaa cgtgcccaga gccaaagata aaatgacttt tttcatacca ccatcacaac
     3781 caccagaacg gctaccaatc accgtagcca ataaagtgat agcttgcagg ataacgacca
     3841 gcaaggccaa gagataattc gcccctctgt attcattatc ctgctgaata gttatttcac
     3901 tgcaaacgta ctttccagtt tgctgtagcc caggccgttg atctttttca ctttgatctg
     3961 caccggaata cgctctttca tcgcttctac gtggctaatc acaccgatgg ttttgccact
     4021 ggcgttcagg gcatccagcg catcaagggc ggtatccagc gtttcgctat ccagcgtgcc
     4081 aaaaccttca tcaaggaaca gcgagtcaat acgtgtttta tggctgacca gatccgaaag
     4141 cgccagcgcc agcgccagac taacgaggaa actttcgccg ccggaaaggg tacgggtatc
     4201 gcgtaccgca tctgcctgcc aggtatcaac aacctcgact tccagcgcct cgctggcttt
     4261 gcgctgtaac agatagcgcc cgtgcagccg ggtaagttgc tgattagcga gatggactaa
     4321 attatccagc gtcagcccct gggcaaactt gcggaattta tcgccctctt tggaacctat
     4381 tagcgaattc agatatcccc agtcctcaac ctgctgcgtc atttgagcaa tttgctgcat
     4441 taaggtttgt tgttgctgac ggttatctgc atcctgcttc agctgctggc gaatctcgcc
     4501 ttgactcgtg gtgttttcac gcaacttttg gtgagtttgc gctaactctt gctgaatctg
     4561 ctccaccgtc acagtgagag ccaacccgtc aggtcggtgt tgttgatgct gtgccagcgt
     4621 ttctgctgtc tgagtgacca gcgtttgcgc ctgacggcgc tggttttcca gattctgctt
     4681 gagctgttcc agctgcgtta gtgtttgttc atccattagc gccgcaagga acgcctgctg
     4741 atcgtcaaag acgctggcct gtagcgtggt gtcaaactgc gcttgggctt tttgcagact
     4801 ttgcgccgcc agaacatcct gttgctgtaa cgtctgctgc tggctgtgta atgcgagaca
     4861 ttgttcatga acctgccgcc agttatccag cgccacagtt tcttcgctgt gcgggagatc
     4921 atcactttgc ggcaacgttt ccagaatcgg cgtcagctgc tgaatacggt tttgcagcgc
     4981 ggttaattcg ttctggcgtt gctgccagct ctgcgcttct tgctgacgtg tcgccaacca
     5041 gctctcttct tcatcttcct gtggcaatgt cagtgcataa cccgccaatg ccgttaagag
     5101 ctgctgctga cgttgttcaa tttgctgttg atactggata atttgctgat tatgcgcggc
     5161 aatctgccct tgtaattcat gtcgttggct gagtaaccgc agctggcgtt cgtgctcatc
     5221 ttgtgcatcc agccacggtt gaatatcgtc ctgtggctgc aaggtgatat tgaggctggc
     5281 cgtgacggct tgccattgtt gagtaagtgc ttgctcatct tgtcggaggc tttgcgcttc
     5341 gttttcatcg cgctgaagct gcttcgttaa tgcatccagt tgcccgcgca gcgccgcacc
     5401 ttcttcgccg agctttttaa cttcgttttc cagcgccagt aatcgagact gattaacacc
     5461 aggctccagc gcctgatacg cctcgaccgc cgggtggctg gtggaaccac aaagtggaca
     5521 aggctgaccc gcctgtaact gtgcacgctg ggcttccagc gttttgatgc gcgcttcctg
     5581 ctcgcaaatg gttttcacat cggcaagttg ctgcgtcttt tctttataac gctggcgcat
     5641 ttcgttaagt gcgacattac gttgcgtctg ttccagagtg acattctgga tagtgaccat
     5701 taactgcgcc agacgttttt gttggggaac aatctgccca tgcagcgcga ccaggcgctg
     5761 acgcagtggg cgttgctcgg catgttgcgc cagggcggta gcaacttcat cggcggttaa
     5821 cgtcaacgtg atcgccgcaa gcgcattaag tttttgctca gcatgggtta actgttgctg
     5881 ccattgccgc agatgctcgc gatcgctggt ttgttgggag aactgcgcac gccaacccgc
     5941 cagttcgttg ttccactgac ggaagcggtc gtgttcctgt aaccaggtat tcaggctttg
     6001 ttgctgctgc tgtaattctg ctgactgctt cgccgcgtgg tggcgaatgc tcgcgcgaag
     6061 cgccattgtg ctctgtaagc gagtatttac ttcttcaatc tgctggcgaa tatgcgccag
     6121 cgccgcgctg tgttctgcga tgcgttccca gtgtggacga agatttcgtg ccggttgtgc
     6181 cagactaagc gccgccagtt gaggttgcgc tttttcttct tcggctaacg cctgttgcaa
     6241 ggcctgctga cggcggctgg cttcttgctg caattcgtcc tgacgcgtta accagtttag
     6301 cgattgttgt tcttgctgct gcgcggtaat taactgtttt tcttcgtcag taagtacctg
     6361 caaactcgct gtcagcgatt gcacttgttc cggcgtgagc aacgtgacgc cgctggcctg
     6421 cgcttgcagc ttctccagct cagtacgggc cgatttgtgc tgctcaaaaa ccatcgccga
     6481 gatttgcccg tagatttcag tgccggttaa ctcctcgagc aattccgcgc gttctttggg
     6541 tttggcattc aggaaggcag caaattgccc ctgcgaaagc agcatcgaac gggtgaagcg
     6601 cccgtaatcc agcccggtta acgtcgctgt cagttccagc ttatctttca ctttgtcggc
     6661 gagaattttg ccgtcggcgc agcgcgccag ctctacgcga ggcacctgca aattaccgtc
     6721 cggctggtta cgcgcccgat tctggctcca gaacgcgcga tacgcttcac ctttcacttc
     6781 aaactccacc tccgccagac attcagcggt atcgcgggtc atgagatcat tttgcgattg
     6841 tgaaacgtta gagagacgcg gagtttcgtg atacagcgcc agacaaatgg cgtccagcag
     6901 ggtggttttc cccgcaccgg ttgggccggt aatggcaaac agcccgttgc tggcgaacgg
     6961 ctcgcgggtg aaatcaatct tccattcgcc ttttaatgag ttcaggtttt tcaggcgcag
     7021 gctgagaatt ttcatgcttc gtgttctccg gcgagggtat gcaacgtcgt ggtgaaaaga
     7081 tgctgcagac gttgctgctg cgattcatcc agttcttcca gtgccagacg gcgattgaac
     7141 acctcttcga cgctgagttc gctgagggtt tcacgctgtt ggctggctaa cacacgctcg
     7201 cgctgttcac gactccgacg taccagcaat acttcgacag gcaatgattc ggttaatgcc
     7261 tggattttgc gctgaatatc atgcagatac tcatcagtag tgatttcgat atccagccag
     7321 acaggtggct cctgcgatac atcgcgccac tgttccagct gtgcggtaat cgacgccaga
     7381 tcgcctttca gcaccgccat gggttgagtt accggtacgt tcaggttttc cacgctctct
     7441 aatttgccgt ttgaaaatgt caccagatgg acatatttac tcttaccgca ttcatcaaaa
     7501 ctcagtggaa tgggggaacc gcaatagcga acatgttcca tgccgccaat aatctgtgcg
     7561 cggtgaatat gcccgagcgc gatgtagtcg gctggtggaa agttttgtgc cggaaacgcg
     7621 tccagcgtgc caatataaat gtcacgcacg gcgtcacttt tactggcacc cacggtcgtt
     7681 aaatgtcccg tggcgatgat gggcagaggc tgatcgccgc gcagtttgca ggcatcggca
     7741 tagtgttgtt ggtaataatc ggtaatcgct gccagtaaat gctgctgttt ttcaataccg
     7801 ttaagccccg cctggctgga aataatgtca cgcgggcgta aaaacggaat ggggcacagc
     7861 actgcgcctg gcgtcccgtc gcgacgaggc aagatttgcg gcgcatgtcc ggcgctggcg
     7921 accacggttg tattgaggaa cgccatgata tcgcgcgatt cattcagcgt ggcgaccgag
     7981 tcatggtttc ctgccagtac caccagatga cagccagttt gctgtaaatt gacaacaaaa
     8041 cggttgtata acgtgcgggc gtaactgggc ggcgagccgg tatcgaaaac atcaccagca
     8101 acaataatcg catccacctg atgggtttgt gctgtctcca gcagccagtc aagaaaagcc
     8161 tgatgttcag cttcgcggct tttactgtag aagttctggc cgagatgcca gtctgaggtg
     8221 tgaaggatgc gcataacggt tccctggcga aaaagcatgg tcgcgattat acccaaacag
     8281 atgtgccatt tgcttttttc tgcgccacgg aaatcaataa cctgaagata tgtgcgacga
     8341 gcttttcata aatctgtcat aaatctgacg cataatgacg tcgcattaat gatcgcaacc
     8401 tatttattac aacagggcaa atcatggcga gacgtattct ggtcgtagaa gatgaagctc
     8461 caattcgcga aatggtctgc ttcgtgctcg aacaaaatgg ctttcagccg gtcgaagcgg
     8521 aagattatga cagtgctgtg aatcaactga atgaaccctg gccggattta attctccttg
     8581 actggatgtt acttggcggc tccggtatcc agttcatcaa acacctcaag cgcgagtcga
     8641 tgacccggga tattccagtg gtgatgttga ccgccagagg ggaagaagaa gatcgcgtgc
     8701 gcggccttga aaccggcgcg gatgactata tcaccaagcc attttcgccg aaggagctgg
     8761 tggcgcgaat caaagcggta atgcgccgta tttcgccaat ggcggtggaa gaggtgattg
     8821 agatgcaggg attaagtctc gacccgacat ctcaccgagt gatggcgggc gaagagccgc
     8881 tggagatggg gccgacagaa tttaaactgc tgcacttttt tatgacgcat cctgagcgcg
     8941 tgtacagccg cgagcagctg ttaaaccacg tctggggaac taacgtgtat gtggaagacc
     9001 gcacggtcga tgtccacatt cgtcgcctgc gtaaagcact ggagcccggc gggcatgacc
     9061 gcatggtgca gaccgtgcgc ggtacaggat atcgtttttc aacccgcttt taacgccttg
     9121 ctcatcggac gcagagcagg gcttatgatt tcttaactgg agtatcttac gtgctggaac
     9181 ggctgtcgtg gaaaaggctg gtgctggagc tgctactttg ctgcctcccg gctttcatcc
     9241 tgggtgcatt ttttggttac ctgccctggt ttttgctggc atcggtaaca ggactgctta
     9301 tctggcattt ctggaattta ttgcgccttt catggtggct gtgggtggat cgcagtatga
     9361 ccccgccacc ggggcgtggt agctgggaac cgctactata cggcttacac cagatgcagc
     9421 tgcgaaataa aaaacgccgc cgtgaactgg gcaatctgat taaacgcttt cgtagcggcg
     9481 cggagtcgct gcccgacgcg gtggtgctga ccacggaaga gggcggtatt ttctggtgta
     9541 acggtctggc gcaacaaatt cttggtttgc gctggccgga agataacggg cagaacatcc
     9601 ttaacctact gcgttacccg gagtttacgc aatatctgaa aacgcgtgat ttttctcgcc
     9661 cgctcaatct ggtgctcaac accgggcggc atctggaaat tcgcgtcatg ccttataccc
     9721 acaaacagtt gttgatggtg gcgcgtgatg tcacgcaaat gcatcaactg gaaggggcgc
     9781 ggcgtaactt ttttgccaac gtgagccatg agttacgtac gccattgacc gtgttacagg
     9841 gttacctgga gatgatggat gagcagccgc tggaaggcgc ggtacgcgaa aaagcgttgc
     9901 acaccatgcg cgagcagacc cagcggatgg aagggctggt gaagcagttg ctaacgctgt
     9961 cgaaaattga agcctcgccg acgcaattgc tcaatgaaaa ggttgatgtg ccgatgatgc
    10021 tgcgcgttgt tgagcgcgag gctcagactc tgagtcagaa aaaacagaca tttacctttg
    10081 agatagataa cggcctcaag gtgtctggca acgaagatca gctacgcagt gcgatttcga
    10141 acctggtcta taacgccgtg aatcatacgc cggaaggcac gcatatcacc gtacgctggc
    10201 agcgagtgcc gcacggtgcc gaatttagcg ttgaagataa cggaccgggc attgcaccgg
    10261 agcatattcc gcgcctgacc gagcgttttt atcgcgttga taaagcgcgt tcccggcaaa
    10321 ccggcggtag cggattaggg ttagcgatcg tgaaacatgc ggtgaatcat cacgaaagtc
    10381 gcctgaatat tgagagtaca gtaggaaaag gaacacgttt cagttttgtt atcccggaac
    10441 gtttaattgc caaaaacagc gattaatccg cctttgtcat cttttattgc cataagccag
    10501 ccgatgctgg cttattttct ttgcagtcaa aatacgggcg ttagatttta caacggttgg
    10561 tgattttttg ttcgcatgat tagccatgtc tttttcacgg aaatagtgtt ttatactggt
    10621 tggtgatttc ttatcgctat atacctctgg tttttagatc cctccttgct ttaaaacgtt
    10681 ataagcgttt aaattgcgct tcaggtgctg tcatactgac tgcattaacg cggtaaatcg
    10741 aaaaactatt cttcgccgcg cctggttggg agtatttccc gctaaaattg tttaaatata
    10801 ccgctgtatc atccccaggg attggcacaa aaatttaacg ttacaacacc acatccacag
    10861 gcagtatgat ttatgaccca tcaattaaga tcgcgcgata tcatcgctct gggctttatg
    10921 acatttgcgt tgttcgtcgg cgcaggtaac attattttcc ctccaatggt cggcttacag
    10981 gcaggcgaac acgtctggac tgcggcattc ggcttcctca ttactgccgt tggcctgccg
    11041 gtattaacgg tagtggcgct ggcaaaagtt ggcggcggtg ttgacagcct cagcacgcca
    11101 atcggtaaag tcgctggcgt actgctggca acggtttgtt acctggcggt ggggccgctt
    11161 ttcgctacgc cgcgtacagc taccgtttcc tttgaagtgg ggattgcgcc gctgacgggt
    11221 gattccgcgc tgccgctgtt tatctacagc ctggtctatt tcgctatcgt tattctggtt
    11281 tcgctctatc cgggcaagct gctggatacc gtgggcaact tccttgcgcc gctgaaaatt
    11341 atcgcgctgg tcatcctgtc tgttgtcgcg attgtctggc cggcgggttc tatcagcacg
    11401 gcgactgagg cttatcaaaa cgctgcattt tctaacggct tcgttaacgg ctatctgacc
    11461 atggatacgc tgggcgcaat ggtgtttggt atcgttattg ttaacgcggc gcgttctcgt
    11521 ggcgttaccg aagcgcgtct gctgacccgt tataccgtct gggctggcct gatggcgggt
    11581 gttggtctga ctttgctgta cctggcgctg ttctgtctgg gttcagacag cgcgtcgctg
    11641 gtcgatcagt ctgcaaacgg cgctgctatt ctgcatgctt acgttcagca cacctttggc
    11701 ggcggcggta gcttcctgct ggcggcgtta atcttcatcg cctgtctggt cacggcggtt
    11761 ggcctgacct gtgcttgtgc agaattcttt gcccagtacg taccgctctc ttatcgtacg
    11821 ctggtgttta tcctcggcgg cttctcgatg gtggtgtcta acctcggctt aagccagctg
    11881 atccagatct ccgtaccggt gctgaccgct atttatccgc cgtgtatcgc actggttgta
    11941 ttaagtttta cacgctcatg gtggcataat tcgtcccgcg tgattgctcc gccgatgttt
    12001 atcagcctgc tttttggtat tctcgacggg atcaaagcat ctgcattcag cgatatctta
    12061 ccgtcctggg cgcagcgttt accgctggcc gaacaaggtc tggcgtggtt aatgccaaca
    12121 gtggtgatgg tggttctggc cattatctgg gatcgcgcgg caggtcgtca ggtgacctcc
    12181 agcgctcact aaatcactga acatttgttt taaccacggg gctgcgatgc cccgtggttt
    12241 tttattgtgt tgatgggtta ggaattgatg gaaagtaaga ataagctaaa gcgtgggcta
    12301 agtacccgcc acatacgctt tatggcactg ggttcagcaa ttggcaccgg gctgttttac
    12361 ggttcggcag acgccatcaa aatggccggt ccgagcgtgt tgttggccta tattatcggt
    12421 ggtatcgcgg cgtatatcat tatgcgtgcg ctgggggaaa tgtcggtaca taacccggcc
    12481 gccagctctt tctcgcgtta tgcgcaggaa aaccttggcc cgctggcagg ttacattacc
    12541 ggatggacct actgctttga aatccttatt gtcgccatcg ccgatgtgac cgcttttggt
    12601 atctatatgg gtgtcttgtt cccgacggtg ccgcactgga tttgggtact gagcgtggtg
    12661 ctgatcattt gcgccgtaaa cctgatgagc gtgaaggtct tcggtgagct ggaattctgg
    12721 ttctcgttct ttaaagtcgc caccatcatc atcatgattg tcgccggttt cggcatcatc
    12781 atctggggga ttggcaacgg cgggcaaccg accggtattc ataacctgtg gagcaacggc
    12841 ggcttcttca gtaacggctg gcttggcatg gtaatgtcgt tgcaaatggt gatgtttgct
    12901 tacggtggga tcgaaattat cgggattacc gccggtgaag cgaaagatcc tgagaaatcg
    12961 attccgcgtg cgattaactc cgtgccgatg cgtattctgg tgttctacgt cggtacgctg
    13021 ttcgtcatta tgtctatcta cccgtggaat caggttggca ctgccggtag cccgttcgtg
    13081 ctgacgttcc agcatatggg cattaccttt gccgccagca ttcttaactt tgttgtgctg
    13141 accgcttcgc tgtcggcaat taacagtgac gtatttggcg taggccgtat gctccacggt
    13201 atggcagagc agggcagcgc accgaaaatt ttcagcaaaa cgtcgcgtcg cggtattccg
    13261 tgggttacgg tgctggtgat gactaccgcg ctgctgtttg cggtgtatct gaactacatc
    13321 atgccggaaa acgtcttcct ggtgatcgct tcgctggcaa ccttcgccac ggtatgggtg
    13381 tggattatga tcctgctgtc gcaaatcgcc ttccgtcgcc gtttgccgcc agaagaagtt
    13441 aaggcgctga aatttaaagt gccgggtggg gtagcaacga ccatcggcgg gctgattttc
    13501 ctgctcttta ttatcgggtt gattggttat cacccggata cgcgtatctc gctgtatgtc
    13561 ggtttcgcgt ggattgttgt gctgttgatt ggctggatgt ttaaacgccg ccacgatcgt
    13621 cagctggctg aaaaccaata atacttgcgt tctgtaagcc ggttcaggag gaaatcctga
    13681 tccggctatt ccgaaagtta tccgcccccg tcctcctccc ccaaatatcc ttcagatgat
    13741 gagtgatcct gcattaggct atggcaaggt gatcagattt tcatcacagg ggaattatga
    13801 tgttaaatgc atggcacctg ccggtgcccc catttgttaa acaaagcaaa gatcaactgc
    13861 tcattacact gtggctgacg ggcgaagacc caccgcagcg cattatgctg cgtacagaac
    13921 acgataacga agaaatgtca gtaccaatgc ataagcagcg cagtcagccg cagccaggcg
    13981 tcaccgcatg gcgtgcggcg attgatctct ccagcggaca accccggcgg cgttacagtt
    14041 tcaaactgct gtggcacgat cgccagcgtt ggtttacacc gcagggcttc agccgaatgc
    14101 cgccggcacg actggagcag tttgccgtcg atgtaccgga tatcggccca caatgggctg
    14161 cggatcagat tttttatcag atcttccctg atcgttttgc gcgtagtctt cctcgtgaag
    14221 ctgaacagga tcatgtctat taccatcatg cagccggaca agagatcatc ttgcgtgact
    14281 gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc gatctggacg
    14341 ggataagcga aaaactgccg tatctgaaaa agcttggcgt gacagcgctg tatctcaatc
    14401 cggtgtttaa agctcccagc gtacataaat acgataccga ggattatcgc catgtcgatc
    14461 cgcagtttgg cggtgatggg gcgttgctgc gtttgcgaca caatacgcag cagctgggaa
    14521 tgcggctggt gctggacggc gtgtttaacc acagtggcga ttccca
//
LOCUS       DAJTYI010000098        14542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYI010000098 DAJTYI010000000
VERSION     DAJTYI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            227..424
                     /locus_tag="OO564_002877"
     CDS             227..424
                     /locus_tag="OO564_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653937.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(374..1567)
                     /locus_tag="OO564_002878"
     CDS             complement(374..1567)
                     /locus_tag="OO564_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6653938.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1703..3427
                     /gene="iucA"
                     /locus_tag="OO564_002879"
     CDS             1703..3427
                     /gene="iucA"
                     /locus_tag="OO564_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6653939.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3428..4375
                     /gene="iucB"
                     /locus_tag="OO564_002880"
     CDS             3428..4375
                     /gene="iucB"
                     /locus_tag="OO564_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6653940.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4375..6117
                     /gene="iucC"
                     /locus_tag="OO564_002881"
     CDS             4375..6117
                     /gene="iucC"
                     /locus_tag="OO564_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6653941.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6114..7430
                     /gene="iucD"
                     /locus_tag="OO564_002882"
     CDS             6114..7430
                     /gene="iucD"
                     /locus_tag="OO564_002882"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6653942.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7436..9631
                     /gene="iutA"
                     /locus_tag="OO564_002883"
     CDS             7436..9631
                     /gene="iutA"
                     /locus_tag="OO564_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6653943.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10182..10325)
                     /locus_tag="OO564_002884"
     CDS             complement(10182..10325)
                     /locus_tag="OO564_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653944.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10575..14462
                     /gene="sat"
                     /locus_tag="OO564_002885"
     CDS             10575..14462
                     /gene="sat"
                     /locus_tag="OO564_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR6653945.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3875 a         3489 c         3638 g         3540 t
ORIGIN      
        1 gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc ctgataccca
       61 tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac gcgcagcgca
      121 gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca gagcaacgct
      181 gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg agattacacc
      241 gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca gaacactgcc
      301 tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact ccagtaaggt
      361 gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac acaatggtaa
      421 ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct gttttcgata
      481 ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta gcgagccagc
      541 ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg ggtgttgcca
      601 gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc tgttcggaga
      661 gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg gccaccggag
      721 tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact gcggacagac
      781 ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac tggcgagtaa
      841 gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat attgcgcaaa
      901 gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt gtgggctgtg
      961 gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct gccatcagca
     1021 gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc ggcccgaata
     1081 gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1141 cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg gtcagcccgt
     1201 aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg gcgctggtag
     1261 ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct cccagagccc
     1321 acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac ggggacgatg
     1381 gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga ctcagatttg
     1441 tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata ccgagaagcc
     1501 cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct tcgatccctg
     1561 gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa attaatttat
     1621 ctgacaataa catttattgt tgataatgag aatcattatt gacataattg ttattatttt
     1681 actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca cagatgtggc
     1741 tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac tgacagaata
     1801 cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt tcagagtggc
     1861 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg tcacggcatc
     1921 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctacgcca
     1981 tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc tggaaagcca
     2041 tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc gtgaaaaagc
     2101 gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc accctgcgcc
     2161 taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg atatggcacc
     2221 ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg aaagtttgca
     2281 tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc agttactcaa
     2341 cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg aatatctttt
     2401 gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact taggtgaggc
     2461 cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca ccagccgcga
     2521 tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc tgtccgtgaa
     2581 agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct ggcagatgct
     2641 acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg ggctgctcga
     2701 tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc tgctggtgga
     2761 tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac ctgatggcgg
     2821 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     2881 acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc cgctgtttac
     2941 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtccagat
     3001 gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg cttttatgcc
     3061 tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata ttttcaccca
     3121 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgctgtgac
     3181 cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc gtgtacgagc
     3241 atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact acgtgctgga
     3301 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3361 caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc aggctcagga
     3421 ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct gtgaaaaact
     3481 cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc actgtcccgg
     3541 ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg ccgcaccaca
     3601 actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg cgctgacgct
     3661 ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt taccgttatg
     3721 gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag agcgtgagat
     3781 ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt acgatccacg
     3841 tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     3901 ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     3961 acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc tgattgggtg
     4021 cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag accgcattgg
     4081 tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4141 atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt acctgctgct
     4201 ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4261 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataagcg
     4321 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4381 cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc tgagatggag
     4441 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat taacctgccg
     4501 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg gattgatgcc
     4561 caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat gcagctaaag
     4621 ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt gtatgccacg
     4681 ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag tgacctgatt
     4741 aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     4801 aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga gtataccaac
     4861 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgcgataac
     4921 gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt tacacgcttc
     4981 agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc ggtacatccg
     5041 tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga aggcaggatg
     5101 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5161 aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta caatacctca
     5221 tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc acgctggctg
     5281 caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat ccttggtgaa
     5341 ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5401 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5461 gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5521 ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca attgttccgg
     5581 gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacac
     5641 ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct gctgaaagac
     5701 ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc tttgcctcag
     5761 gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga tttgcagacg
     5821 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     5881 gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa caaacatcca
     5941 caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat tcgcgtggtg
     6001 ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat gctgccgaat
     6061 taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6121 aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt gctgcattgt
     6181 cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat ttttcctggc
     6241 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6301 gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag cacaaaaagt
     6361 tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt tctgactatc
     6421 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaatattg
     6481 atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa tattttgccc
     6541 acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatgtga
     6601 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg agtggaaaac
     6661 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     6721 gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat tttaacgcac
     6781 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     6841 gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     6901 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     6961 gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg gaaagtagtg
     7021 gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag agcctggaaa
     7081 gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata cttccctcac
     7141 tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7201 tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc atgcaaactc
     7261 atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt attcttaatc
     7321 gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt cagtggcgca
     7381 gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa cgatgatgcg
     7441 caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt caccagctgt
     7501 cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca atcgaactgt
     7561 agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc agcagattca
     7621 gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg atgtcagcag
     7681 ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg tcctgattga
     7741 cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg tcgatccttt
     7801 taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg gcgggagtac
     7861 cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga tggagtttga
     7921 ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca ttgccggtgc
     7981 tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc agaaatttgg
     8041 cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga ccggcctgca
     8101 gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg aatcccggca
     8161 gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg ggcttaatct
     8221 cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta aggggctgaa
     8281 ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact ctgacagtga
     8341 tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt tgcggtacta
     8401 cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt cacagcagga
     8461 taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg gctggcaaat
     8521 cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt tcttcgatct
     8581 ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca ccgggcgcta
     8641 tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg acattaatga
     8701 tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg tagatgattt
     8761 catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg ccgacgccat
     8821 tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc tgatgcacat
     8881 caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc cggatccggg
     8941 gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc ccctgacaaa
     9001 gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt atgaactggg
     9061 ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact cgctttccaa
     9121 taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca ggcgccgtat
     9181 ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga gtaccggtgt
     9241 gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa aatatgacgt
     9301 gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg aaccgtggag
     9361 tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta acgatattaa
     9421 tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa cactcagctt
     9481 cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc gtgcacctct
     9541 gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc ggggccgaac
     9601 ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca agggtatgct
     9661 gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat acagccttaa
     9721 caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg tctgttttat
     9781 ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga aaactaacct
     9841 tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga gaggatgatc
     9901 tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa aatgggggag
     9961 tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt tctccgttca
    10021 gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga gattgttaat
    10081 acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta gctacatacg
    10141 gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc catggagtag
    10201 cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag aacttcttcg
    10261 gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac tgaacgatgt
    10321 tccatgcgaa cagatattcg atcatatcga aatattaaac actacccctc cccacattgg
    10381 gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca cataatctct
    10441 catcattaat gagaacattg tcacatattt acaatataga actgttactt ttagcaagct
    10501 gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat tcaataaatg
    10561 agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc actggcggac
    10621 tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga aaacttgtag
    10681 caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt gatatatcaa
    10741 atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc cagcccggag
    10801 caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat aatctctcaa
    10861 ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga ggttcttata
    10921 gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag gtttacgctc
    10981 agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt cagaggttaa
    11041 ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct acaacatatt
    11101 ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa atcataggtt
    11161 ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc tcaacaaatt
    11221 cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa tgggactcat
    11281 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
    11341 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
    11401 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
    11461 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
    11521 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
    11581 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
    11641 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
    11701 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
    11761 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
    11821 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
    11881 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
    11941 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
    12001 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
    12061 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
    12121 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
    12181 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
    12241 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
    12301 tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
    12361 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
    12421 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
    12481 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
    12541 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
    12601 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
    12661 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
    12721 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
    12781 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
    12841 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
    12901 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
    12961 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
    13021 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
    13081 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
    13141 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
    13201 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
    13261 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggctcaagca gatcagcttg
    13321 ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc attgagaaaa
    13381 atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga actgcagtag
    13441 atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg gttatcgagc
    13501 aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct gtggccaacg
    13561 ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc ttccttgctg
    13621 aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt gagtccggtg
    13681 catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt gacaactaca
    13741 cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac ctcttcaccg
    13801 gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt ggtgaaacga
    13861 agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca tatatcgacc
    13921 tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc ggccttggca
    13981 ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac cgttaccatg
    14041 taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct gtatccggga
    14101 aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag gactttaatc
    14161 cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag gactggaaag
    14221 tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt gaaaccgtac
    14281 tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt atgctcatga
    14341 atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag tttgagaaat
    14401 cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt tactctttct
    14461 gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat gtattaccct
    14521 gtgacagact atatcgctct tg
//
LOCUS       DAJTYI010000099        14161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYI010000099 DAJTYI010000000
VERSION     DAJTYI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..964)
                     /locus_tag="OO564_002886"
     CDS             complement(<1..964)
                     /locus_tag="OO564_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6653946.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLI"
     gene            complement(978..2480)
                     /locus_tag="OO564_002887"
     CDS             complement(978..2480)
                     /locus_tag="OO564_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6653947.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(2620..3577)
                     /gene="ytfQ"
                     /locus_tag="OO564_002888"
                     /pseudo
     CDS             complement(2620..3577)
                     /gene="ytfQ"
                     /locus_tag="OO564_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            3887..4417
                     /gene="ppa"
                     /locus_tag="OO564_002889"
     CDS             3887..4417
                     /gene="ppa"
                     /locus_tag="OO564_002889"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6653948.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(4797..5138)
                     /locus_tag="OO564_002890"
     CDS             complement(4797..5138)
                     /locus_tag="OO564_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6653949.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5141..8920)
                     /gene="tamB"
                     /locus_tag="OO564_002891"
     CDS             complement(5141..8920)
                     /gene="tamB"
                     /locus_tag="OO564_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6653950.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(8917..10650)
                     /gene="tamA"
                     /locus_tag="OO564_002892"
     CDS             complement(8917..10650)
                     /gene="tamA"
                     /locus_tag="OO564_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6653951.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            10856..11494
                     /gene="msrA"
                     /locus_tag="OO564_002893"
     CDS             10856..11494
                     /gene="msrA"
                     /locus_tag="OO564_002893"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6653952.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            11817..13160
                     /locus_tag="OO564_002894"
     CDS             11817..13160
                     /locus_tag="OO564_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6653953.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13222..13428)
                     /locus_tag="OO564_002895"
     CDS             complement(13222..13428)
                     /locus_tag="OO564_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6653954.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            13753..>14161
                     /locus_tag="OO564_002896"
     CDS             13753..>14161
                     /locus_tag="OO564_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6653955.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGV"
BASE COUNT         3275 a         3906 c         3526 g         3454 t
ORIGIN      
        1 caatcagcac acaaagcacc accaccgctt tcaccaccag gttcatctcc ggcggaaagc
       61 ccgaaagcaa aattccggtg ttcatcccct gaataatcag cgcccccact accgagagca
      121 gcaggttaaa gcgcccgccc atcagcgatc cgccgccaat caccaccgcg agaatggcgt
      181 ccagctccag ccataatccg gcgttgttgg catcggcacc gcaaatatcc gccgccacga
      241 taatgcccgc aatcgccgca catagcccgc tcaacacgta ggtgagcatg acgatgattc
      301 gcgtgtttac cccggcattt tttgccgccc gaatgttgat accaacgact tcgataaaca
      361 tccccagcgc cgttttgcgg gtcaacagcc agaacaggag aagcgtcagc accgcgataa
      421 tgaccggcgt tggcaggaac aacagcgatc cgctgccaaa ccatgagaga tccggcgagt
      481 taaacgtgac gatctgcccg gcggtgatca gttgcgccac gccgcgcccg gcgaccatca
      541 ggatcagggt ggcgacaaac ggctgaattt tgaggatcgc taccagtatg ccgttccaca
      601 atcccgccag gatgccggtg cccagggcgc ttaacaaaac aatcggcagg ctgaatcccg
      661 cgaccgtcat cgcagccgtt gtggctccgg cgatagccat caccgccccc acagagagat
      721 cgataccacc ggtggcgatc accagcgtca taccaatcgc cagtaacgca acgggagctg
      781 cacggttaag aatgtcgata gggctaccga acaaacgccc atcctgaagc accacctgcc
      841 agaaatgcgg ggctaccagg ctatcgacca gcagcaccag caacagtgcc gccagctgcg
      901 gcatccccgt cggccagtga aaacgccttt ttggcggcgt agtgtcaggg agagattgag
      961 gcatcacact gttctcctta cgccgcaatg gcgttcatga tcgccggaac ggaaagctgt
     1021 gccagcggga tctccgccac ctgtttgcga tcgcgcatga tgatcacccg gtcggcatag
     1081 cccaccagct cttccagttc ggaggagatc accagcagcg ccagaccgtc agcacacagc
     1141 gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat caatgccacg ggttagctca
     1201 tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg aaagcagcac tttttgctga
     1261 ttgccaccgg agagaaattc aatcggttgt tcagttgaag gtgtgcgaat gccaagctgg
     1321 cggataaagc gttcggcaat ctcttgctgc tctttgcggg aaatgggtcg tagccagccg
     1381 cgctgggcct ggagagcaag gatgatattt tcccgcaccg aggcggcggc gatgatgcca
     1441 tcggttttcc tgtcttccgg gcagaatccg atgcccagta ctgaagcctg atgtggcgat
     1501 cgcagggttt gcggtttgcc tttgatcaac gccgtgccgc tgtcagcagg tttgataccg
     1561 aagatcactt cggcggtttc ggtacgtccg gatcccagca agccagccag accgacgatc
     1621 tcgccggggc gcacttcgag atcaaacggt gctatcgttc cttttttgcc gtaatttttg
     1681 aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg cacgctgtag cgcgtgggta
     1741 tccagctcgc gccccagcat catttttacc agttcgatct gcggtagctc gcgcgtttcc
     1801 cgacagccta cgaaactgcc gttgcgtaag acggtgatcc gatcgctgac ctgatagacc
     1861 tgatcgagaa agtgagtgac aaagatcagg ctgacgccgc gatcgcgcaa ctgacgcatc
     1921 aggccaaata atagctccac ttcttgggtg ttgaggctgg cggtgggttc atcgaggatc
     1981 agcactttgg cggagagatc gatagcccgg caaatggcga cgatttgctg catcgccacc
     2041 gaaaaacggt taagcggttc gcgcatgtcg agggagaaac cgtaagatgc catcagttcg
     2101 gtggcgcgct tttccatctc tttacggcgt agaaggccga aacgtttggg ttcgcgacct
     2161 atgaatagat tatcagcaac cgacatattg ggtagcaggt tgacttcctg atagacggtg
     2221 ccgataccga gttgttgtgc atgggcggta tttttcggtg agatagtctg gccttccagc
     2281 cagatggtgc cgcgatcggc gtggtataca ccagttaatg ctttgattag cgttgatttt
     2341 cccgccccgt tttcaccaag cagcgccatg atttcgccac ggcgcaggct gaaatcaacg
     2401 ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc cttcggtgcg gaggatctcc
     2461 tggtgttggt cggtcgtcat ggtttccccc ggtgtaaagc gtgctcccag taggcatgat
     2521 aagacgcggt aagcgtcgca tcaggcttca gtgcaccatc gccggatgcg gcgcgagcgc
     2581 cttatccggc ctacgaatcg gcatcaggca tagcaaccct caatacccca tatttttctt
     2641 cttctctaac tcttcttttg cggtatcagg caggtaaagg gtggatttgg ttaacgtcag
     2701 cttttcaggc atggtgccgt cttttttgta tttctccagc gcatcgaaag cgggtcccgc
     2761 catgttcggc gtcagttcaa cactggcgtt cgcttcgcca tccatcatcg ctttgtagat
     2821 gtccggtaca ccgtcaatgg aacccgtgag gatatctttg cccggtttca ggcccgcttc
     2881 tttaattgcc tgaattgcac caatcaccat gtcgtcgtta tgggcgtaaa ccatgcagat
     2941 gtttttgccg ttgttttccg ctttgataaa gctctccatg acttctttgc ctttactgcg
     3001 ggtgaagtca cctgactgcg agcggataat tttgatattt ggcgcattct taatggcttc
     3061 ggcaaagcct ttcttaacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     3121 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     3181 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     3241 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     3301 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttgtgca acgaaggaac
     3361 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     3421 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     3481 atctgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     3541 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     3601 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     3661 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     3721 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     3781 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     3841 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     3901 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     3961 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     4021 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     4081 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     4141 gatccgttgc cgtccggttg gcgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     4201 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     4261 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     4321 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     4381 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     4441 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     4501 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     4561 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     4621 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     4681 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     4741 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     4801 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     4861 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     4921 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     4981 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     5041 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     5101 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     5161 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     5221 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     5281 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     5341 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     5401 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     5461 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     5521 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     5581 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     5641 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     5701 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     5761 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     5821 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     5881 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     5941 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     6001 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     6061 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     6121 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     6181 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     6241 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     6301 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     6361 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     6421 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     6481 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     6541 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     6601 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     6661 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     6721 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     6781 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     6841 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     6901 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     6961 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     7021 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     7081 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     7141 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     7201 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     7261 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     7321 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     7381 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     7441 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     7501 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     7561 aaatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     7621 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     7681 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     7741 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     7801 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     7861 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     7921 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     7981 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     8041 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     8101 tatccaaggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     8161 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     8221 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     8281 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     8341 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     8401 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     8461 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     8521 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     8581 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     8641 taatacaaac gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg
     8701 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     8761 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     8821 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     8881 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
     8941 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
     9001 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
     9061 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
     9121 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
     9181 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
     9241 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
     9301 cgtaccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgttctgcgc
     9361 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
     9421 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
     9481 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
     9541 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
     9601 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
     9661 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
     9721 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
     9781 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgccccc
     9841 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
     9901 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
     9961 aagtcggcgg ttcagttccg ccaaatcttt cgattcatac tcatcgccct ctttaaacgg
    10021 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    10081 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    10141 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    10201 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    10261 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    10321 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    10381 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    10441 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    10501 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    10561 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    10621 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    10681 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    10741 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    10801 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    10861 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    10921 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    10981 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    11041 gttacccggc ctttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    11101 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    11161 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    11221 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    11281 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    11341 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    11401 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg
    11461 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    11521 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    11581 cgtccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    11641 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    11701 tatactagcg actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    11761 acacaaatta atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt
    11821 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    11881 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    11941 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    12001 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    12061 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    12121 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    12181 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    12241 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    12301 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    12361 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    12421 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    12481 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    12541 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    12601 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    12661 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    12721 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    12781 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    12841 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    12901 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    12961 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    13021 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    13081 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    13141 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    13201 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    13261 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    13321 cttatcaaat tcaaaagcgc caacgtcctt gatgtataac cgtccacgga acaggatctt
    13381 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    13441 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    13501 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    13561 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    13621 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    13681 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    13741 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    13801 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    13861 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    13921 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    13981 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    14041 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    14101 ctggagagta ataaaaagct ttatcccagg tcgcagttta ttgtcgatag caatggcgtc
    14161 g
//
LOCUS       DAJTYI010000100        14053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYI010000100 DAJTYI010000000
VERSION     DAJTYI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..922
                     /locus_tag="OO564_002897"
     CDS             <1..922
                     /locus_tag="OO564_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6653956.1"
                     /translation="AQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLII
                     ASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFG
                     KLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPR
                     IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTL
                     FSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLK
                     TNIESLHLELSEDVLAEIEAVHQVYTYPAP"
     gene            complement(955..2148)
                     /gene="lplT"
                     /locus_tag="OO564_002898"
     CDS             complement(955..2148)
                     /gene="lplT"
                     /locus_tag="OO564_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6653957.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2141..4300)
                     /gene="aas"
                     /locus_tag="OO564_002899"
     CDS             complement(2141..4300)
                     /gene="aas"
                     /locus_tag="OO564_002899"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6653958.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4689..5720
                     /gene="galR"
                     /locus_tag="OO564_002900"
     CDS             4689..5720
                     /gene="galR"
                     /locus_tag="OO564_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6653959.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5727..6989)
                     /gene="lysA"
                     /locus_tag="OO564_002901"
     CDS             complement(5727..6989)
                     /gene="lysA"
                     /locus_tag="OO564_002901"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6653960.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7111..8046
                     /gene="lysR"
                     /locus_tag="OO564_002902"
     CDS             7111..8046
                     /gene="lysR"
                     /locus_tag="OO564_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6653961.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8033..8725)
                     /gene="ygeA"
                     /locus_tag="OO564_002903"
     CDS             complement(8033..8725)
                     /gene="ygeA"
                     /locus_tag="OO564_002903"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6653962.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(8854..10242)
                     /gene="araE"
                     /locus_tag="OO564_002904"
                     /pseudo
     CDS             complement(8854..10242)
                     /gene="araE"
                     /locus_tag="OO564_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10557..11318)
                     /gene="kduD"
                     /locus_tag="OO564_002905"
     CDS             complement(10557..11318)
                     /gene="kduD"
                     /locus_tag="OO564_002905"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6653963.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11348..12184)
                     /gene="kduI"
                     /locus_tag="OO564_002906"
     CDS             complement(11348..12184)
                     /gene="kduI"
                     /locus_tag="OO564_002906"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6653964.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12504..13652)
                     /locus_tag="OO564_002907"
     CDS             complement(12504..13652)
                     /locus_tag="OO564_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6653965.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            13907..>14053
                     /locus_tag="OO564_002908"
     CDS             13907..>14053
                     /locus_tag="OO564_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6653966.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWP"
BASE COUNT         3643 a         3835 c         3340 g         3235 t
ORIGIN      
        1 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
       61 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
      121 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
      181 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
      241 cctacagact gattacctcg atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
      301 cttcggcaaa ctcggttata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
      361 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
      421 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
      481 tattgtcacc attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc
      541 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
      601 gctgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
      661 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
      721 cgccagacgt catggccttg atcctgctca gatggcgctc gcgtttgttc gccgtcaacc
      781 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
      841 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tacatcaggt
      901 ttatacttat cctgcaccat aaagattaac gccggttaaa accggcgtta aatattaatg
      961 acggcgctgc cagatccaca gcgccgttat tgccagcgca aatagcgcac cgaagccaat
     1021 gccaatgggc acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa
     1081 caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct
     1141 ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccagcac
     1201 gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg agaaaatcag
     1261 taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac
     1321 cagcttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata
     1381 ggtcggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa
     1441 acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc
     1501 accattgcac catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt
     1561 ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc
     1621 accaccgtag gccagtgcgc atgcggccag ggcgaccagg acatgccagt cagccagcac
     1681 accaccggct acggaaccga gtaaaatcgc cgctatggca gaagcttcca ttaaaccgtt
     1741 agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
     1801 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
     1861 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
     1921 gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga atgtaagcac ctacaaacac
     1981 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
     2041 ggcaaacagt agcgcgttat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
     2101 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
     2161 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
     2221 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
     2281 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
     2341 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
     2401 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
     2461 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
     2521 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
     2581 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
     2641 cgccctcttc gataccaggg accgacaaca ggcgtgcatc cattcctggt agaatacgcc
     2701 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg
     2761 tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
     2821 tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
     2881 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc
     2941 ggtcatacac caagtccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt
     3001 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
     3061 gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
     3121 cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg
     3181 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc aggcgcggca
     3241 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
     3301 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
     3361 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca
     3421 gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgc gcgatggccc
     3481 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
     3541 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
     3601 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
     3661 ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata cgcgcttcca
     3721 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
     3781 ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg aacaagcgac
     3841 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
     3901 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
     3961 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
     4021 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
     4081 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
     4141 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
     4201 gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat
     4261 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
     4321 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
     4381 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
     4441 cgtaccgaat aatctcacca atcaatacct ctgggatcac cactttagca acctgaagcc
     4501 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
     4561 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccactatgaa aacacgccac
     4621 cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat gtaagcgttt acccactaag
     4681 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
     4741 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctg gctgtgcata
     4801 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
     4861 ccactgaaac ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg
     4921 tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt
     4981 accacaacga acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg
     5041 cgttggtcgt ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa
     5101 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
     5161 ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
     5221 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
     5281 attacgatgc ccttgctgaa agtggtattc cggccaatga tcggctggtg acatttggcg
     5341 aaccagacga aaacggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt
     5401 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gttctcaatg
     5461 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
     5521 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc
     5581 aggctgccga actggctttg gcactggcgg ataatcgccc tctcccggaa attactaatg
     5641 tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc
     5701 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg
     5761 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
     5821 ggacggctat tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
     5881 agataatcac ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc
     5941 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
     6001 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
     6061 attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc
     6121 atttgtttga cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga
     6181 cccggttcaa tttccagttt cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg
     6241 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
     6301 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga
     6361 cgcaccatag caccacacac ctgctccaga tgggcataat caacgccaga accaatgtgc
     6421 atgtgaatgc cgactagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga
     6481 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt
     6541 ccgtgaccaa accccggatt aacgcgcagc catacccgat gccctggcga aacctggccc
     6601 agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
     6661 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc
     6721 ggattgtaac ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc
     6781 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
     6841 cgcaccactt caaactgttt cagcgctgct atctgccgac gaataatttg cgcatcgtag
     6901 acccacaccg ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg
     6961 gtatcggtgc tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat
     7021 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgaatctat gcaaaaatga
     7081 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
     7141 atttttcatg cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc
     7201 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
     7261 tttgagcgcg tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
     7321 gtgcaacgat cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag
     7381 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
     7441 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
     7501 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag
     7561 acactccata cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
     7621 gtgttacctc cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag
     7681 ggtgagaatt acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta
     7741 tttactgaac atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
     7801 tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactagat
     7861 tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc
     7921 ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc aggcgtttag tgggcattta
     7981 caagcggggt taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
     8041 gcataaaagc gacagcatcc tcggcatgga tcgacgcggt atcaaacaca ggcaaaacac
     8101 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
     8161 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
     8221 tccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
     8281 gaataagaca attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
     8341 tggtgtaacg cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc
     8401 cggtggcatc cgcaatgtgt aaaaaaggca gagtgcaacg tgactcaatg gcatccgcca
     8461 ctttatgcat cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa
     8521 gcgccgcctc agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt
     8581 ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc
     8641 gctgtttaat gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc
     8701 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
     8761 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa
     8821 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc
     8881 gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag
     8941 ccagaaagta atgcccacaa acgcaatgtt caacgcagtg tagagccaga acgtaccggc
     9001 agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca
     9061 gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga
     9121 gcacaggatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
     9181 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
     9241 gcaatagccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
     9301 tttacgacct gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag
     9361 tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
     9421 gatacgcggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
     9481 caacataccg agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
     9541 gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
     9601 cgtatcgcgc agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag
     9661 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
     9721 aagaacccca acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac
     9781 gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg
     9841 tacttgccat ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc
     9901 ccagcaccac acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag
     9961 aaccgagtac aaacaggatg gccccaggcg gaacgacagc caaccattaa acagcgcacc
    10021 aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac
    10081 aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa
    10141 caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa
    10201 agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag
    10261 agtaagacat tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca
    10321 taaatatgga ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat
    10381 taagttgaat tattgagatt attattaacc acctaatttt aaagtagata aaattcataa
    10441 agttcattaa ttgataattg atatggatta tttcataacc atgatatgga ttatgatgat
    10501 ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg aatgaattaa
    10561 cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc tgaagcgctg
    10621 gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga
    10681 atgcgatcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg ggtattgttg
    10741 gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcgttt
    10801 gccatcaatc gcgtcacacc catcactccg ctttttgatg cggtataaga aggcacacgg
    10861 atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc
    10921 gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc
    10981 atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg aatcaatccg
    11041 gcgttattca ccaggatatc aatatgacca aattcagcta ccgcgcgatc cagcagtcca
    11101 ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc
    11161 gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc
    11221 gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc
    11281 gcaactttac cttggagaga aaatgcactt aaaatcataa caacacctta ttcgtttatg
    11341 cccacaacta gcgcaaatct ttaacggcca catggtccat atcattaaag acctggtttt
    11401 caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa tggatcgacc
    11461 agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct
    11521 gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca
    11581 tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga gccagctccg
    11641 tcagccccat actcaattga caggtttcca gtacatccgg gacaaaatat ttgttaatcg
    11701 tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga
    11761 cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag
    11821 tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca tacagggcgt
    11881 cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca ccgccaatat
    11941 tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt
    12001 ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa
    12061 tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat
    12121 tacgcagccc ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc tgtcttacgt
    12181 ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt
    12241 ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat taaaaataaa
    12301 acaatgtttt atttttaatt taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc
    12361 atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag ccatcattat
    12421 tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag ctactcgtca
    12481 cgttcaatgg tcaatgccac gccctagccc ccgccgatac aaagcgttgc cagccctttg
    12541 cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg
    12601 cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac
    12661 tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga
    12721 tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc
    12781 gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc
    12841 acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca
    12901 tcgtttatgg atgatgcatt ccccgcggtc acagaaccga gactatcaaa tgaaggattt
    12961 aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt atcaacaacc
    13021 aacgtctgac cgttactttg gatcattacc gggacgatct catctttaaa tcgtccggcg
    13081 tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caactgacgg
    13141 ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag
    13201 gcatcccaca acccatcatg caccagactg tcaaccaact ggctattgcc aagctgtgca
    13261 ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccaccggcg
    13321 atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaaga
    13381 ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag accaccttta
    13441 atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt
    13501 acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga
    13561 ctaccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc gataggtgtc
    13621 cgtaacgccc cgacaaccac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa
    13681 tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca
    13741 aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact
    13801 aacccaccaa tagatgggta gatagggcgt taatctccca tacttaactt ggtttatggt
    13861 aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg
    13921 gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg gtgcaggcac
    13981 gctattcctt cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct
    14041 ggtcgcctgg cct
//
LOCUS       DAJTYI010000101        13882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYI010000101 DAJTYI010000000
VERSION     DAJTYI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(658..2118)
                     /gene="phoQ"
                     /locus_tag="OO564_002909"
     CDS             complement(658..2118)
                     /gene="phoQ"
                     /locus_tag="OO564_002909"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6653967.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2118..2789)
                     /gene="phoP"
                     /locus_tag="OO564_002910"
     CDS             complement(2118..2789)
                     /gene="phoP"
                     /locus_tag="OO564_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6653968.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(2958..4328)
                     /gene="purB"
                     /locus_tag="OO564_002911"
     CDS             complement(2958..4328)
                     /gene="purB"
                     /locus_tag="OO564_002911"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6653969.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4332..4973)
                     /gene="hflD"
                     /locus_tag="OO564_002912"
     CDS             complement(4332..4973)
                     /gene="hflD"
                     /locus_tag="OO564_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6653970.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5009..6115)
                     /gene="mnmA"
                     /locus_tag="OO564_002913"
     CDS             complement(5009..6115)
                     /gene="mnmA"
                     /locus_tag="OO564_002913"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6653971.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6169..6630)
                     /gene="nudJ"
                     /locus_tag="OO564_002914"
     CDS             complement(6169..6630)
                     /gene="nudJ"
                     /locus_tag="OO564_002914"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6653972.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(6640..7293)
                     /gene="rluE"
                     /locus_tag="OO564_002915"
     CDS             complement(6640..7293)
                     /gene="rluE"
                     /locus_tag="OO564_002915"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6653973.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7465..8715
                     /gene="icd"
                     /locus_tag="OO564_002916"
     CDS             7465..8715
                     /gene="icd"
                     /locus_tag="OO564_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6653974.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(8818..9141)
                     /gene="iraM"
                     /locus_tag="OO564_002917"
     CDS             complement(8818..9141)
                     /gene="iraM"
                     /locus_tag="OO564_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6653975.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9394..9438)
                     /locus_tag="OO564_002918"
     CDS             complement(9394..9438)
                     /locus_tag="OO564_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653976.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(9674..9784)
                     /locus_tag="OO564_002919"
     CDS             complement(9674..9784)
                     /locus_tag="OO564_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6653977.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(9837..10241)
                     /locus_tag="OO564_002920"
     CDS             complement(9837..10241)
                     /locus_tag="OO564_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6653978.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10462..11193)
                     /gene="bluR"
                     /locus_tag="OO564_002921"
     CDS             complement(10462..11193)
                     /gene="bluR"
                     /locus_tag="OO564_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6653979.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11398..12609)
                     /gene="bluF"
                     /locus_tag="OO564_002922"
     CDS             complement(11398..12609)
                     /gene="bluF"
                     /locus_tag="OO564_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR6653980.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            12923..13159
                     /locus_tag="OO564_002923"
     CDS             12923..13159
                     /locus_tag="OO564_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR6653981.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13202..13474
                     /locus_tag="OO564_002924"
     CDS             13202..13474
                     /locus_tag="OO564_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR6653982.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            13503..13769
                     /gene="ariR"
                     /locus_tag="OO564_002925"
     CDS             13503..13769
                     /gene="ariR"
                     /locus_tag="OO564_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR6653983.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         3709 a         3188 c         3225 g         3760 t
ORIGIN      
        1 cgctctgata ttttcggaaa cctcaatttg aggaagtcgg cgaggtaact gtttgatatt
       61 cccgatagcg caaaaatgcg cggtcgggca gaatgaccac cacctcaatc gtgacggaga
      121 gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa ccgttcatta aaaatcgacg
      181 tgattttgtg ctttccggat gacgtggcca acgtcatttt ttagcgaaaa attctgatta
      241 aggtcactgg ctgaacggtc ataatgacca cgcagaccat cgaggcaacc atagcgatca
      301 tcgttacggt tggagctttt ccgccattgg tggaaaaggt gcacatctgg atcatcatta
      361 cggtgatcaa gggtatgtaa aacccaccag cctgattaaa ggttaaccgg aaaaaaaatc
      421 cggtagcccc gttatgggga tccccatatc gacattaacg cccctcatga attggtgcgc
      481 ttccccctgg aaagattacc cgcctgtata tttcttgtgt ctatttgttc cacgttgttt
      541 catacagtgc accgaacggt gtagttaatg gtgtagtcaa tttgcgattt ttggcacttt
      601 ttcaatgaaa attgtttaat cgaatcaatg acttgatgta gtggtaaaag gacatattta
      661 ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc tccatccgcg caccgcccag
      721 catgctctct ccggcgacga ttttaccctc atattgctcg gtgatttcgc gggctaccgc
      781 cagccctaca ccttgcccag ggcgtaaagt atcaacccgt tgaccacggt cgaaaatgac
      841 ctctcgcttg cttaatggaa taccggggcc atcatcctcg accacaatat agagatgctc
      901 gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa tatttacagg cattatccag
      961 cacgttgccc atcacctcga caaaatcgtt ctgctcaccg acaaagctga tctctggcga
     1021 aatatcgaga gagatattga cccctttgcg ttgatacact ttgttcagcg ctgaggtgag
     1081 attgtccagc agtggggcga ccggatgcag ctcgcggctg agcaatgtcc cgccgcgcat
     1141 actggcacga tgcaggtagt agccaatttg ctgtgaaatg cggctgattt gctccagcat
     1201 taccggctca gcatcactga cgctcatctt ttcactacgc agagaacgca gcgtactttg
     1261 cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg gtgagcgtcg tacggtattt
     1321 gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg tttcgtacca gactggtcag
     1381 ttctcgcgtt gtggctggat tgagcaattc gcggttatgt tcttccagtt cgcggacttc
     1441 ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg gcgacccaca gcagcgggat
     1501 cactaacagc agattggctg agagtacata gataaaccag ctccagacca tataggaact
     1561 ttttagctcc accggaatgg tatccaccac cacaatggtt aattttggca tccgcgatgt
     1621 tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc gcgtcgtcat catcttcccg
     1681 cacttcctgc aactgttgct gtatcgaatg atctccactc agcaagaggc tggtatcgtt
     1741 aacatccgct tcaatttcat gaaaaccatt cgatttcagc cagtcaggct ggatcatttt
     1801 catcagccag ggcacgtcac gttgcgccca taaaagctgc ccgttctcat cataaattag
     1861 cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac tcgacatgca acttattgtt
     1921 ttcccactgc gcaagggtat agaacagatt gctctcgcca cgtaacagcc gaaacgtagt
     1981 tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt ccgtaggcaa gcgaaagcac
     2041 cagtactacc gctgccgttg ccaacagaaa acgtacccgc agcgagagcg ggaaaaaaag
     2101 acgcagtaat tttttcatca gcgcaattcg aacaggtagc cctggccgcg aacggtggta
     2161 atgacttctt gcggatattg tgcctgaatt tttttgcgca gacgtcccat cagtacatca
     2221 atggtatggc tttcccgcag ctccgcatcc ggatagagtt gtagcattaa cgaatctttg
     2281 ctgaccactt tgccattatt gcgtatcaac gtttccataa tggtgtattc gaacgcggtc
     2341 agtttgatca cttcgtcatt aatagataat tcacggcgag agagatcaac ctgaaacggg
     2401 gggagcgaaa tgacctgtga agccagaccg ctattacgcc gcattaatgc ctgcattcgc
     2461 gccatcacct cttcaatatg aaacggttta gtcacgtaat catcagcacc ggcacttaat
     2521 acttcgactt tgtcctgcca gctttcacgg gcggttaata ccagaatcgg cagtgaaaca
     2581 tcgttgctac gccagcggcg aatcagtgac agaccgtcct cgtcaggcaa tccgagatcg
     2641 acaatcgcaa tatccggtaa atgttcattg agataataat cggcttcttt ggcatcttct
     2701 gcgtcatcga cctgatgacc agcatcctga atctgaactt taaggtggtg acgtaacaac
     2761 gcattgtctt caacaaccag tacgcgcatt tttatttctc cctgtcttaa ttattaaaat
     2821 agtgtaacgc gattatgtgt ttatgggggt aaacattaaa taaaccagcg gggaggggag
     2881 gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca ggccggacag catcgccatc
     2941 cggcactgat ttgaggctta tttcagctca tcaaccatcg tgatagctcg accaatatag
     3001 ttagccggcg tcatcgcttt caggcgggct ttctcttctt ctggcaacgc cagaccatcg
     3061 ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc gagtaagctc tttcagcttc
     3121 tcgtacggtt tttcgatgcc atagcgacgc ataactgtct ggattggttc tgccagcact
     3181 tcccagttgt gatccagctc atccagcaga tggtcacggt tcacttccag tttgctcacg
     3241 cctttcaggg tggattgata cgcaatcagc gcataaccga tacccacgcc gaggttacgc
     3301 agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa ccggcagttt gcttgccaga
     3361 tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg agttttcgaa gtcgatcggg
     3421 ttaactttat gcggcatggt ggaagaacca atctcaccag caatggtttt ctgtttgaag
     3481 tggttaaggg cgatataacc ccagacgtca cggtcaaagt cgatcagaat ggtgttgaag
     3541 cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg gttcaatctg ggtagtgtac
     3601 gggttccatt gaatacccag cgaggtgacg aactcttcgc tgaactgatg ccagtcaact
     3661 tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg cgccgttgat tttgccgagg
     3721 atctccacct ggttaagctg gcggtgctgg cgctccatac ggtaggcgac gtttgccatc
     3781 tctttaccga tggttgacgg cgtggctggc tgaccgtggg tacgagacag cagcgggata
     3841 tcgcggtact gaacggcgag atctttaatg ccatcaatca gttgacgcca gtacggcagg
     3901 atcacttcat cacgcgcggt tttcagcatt aatgcgtggg agaggttatt gatatcttcc
     3961 gaagtacagg caaagtggat gaattcagaa accgcgtgca gttccgggat ctccgccact
     4021 ttttctttca ggaaatactc aaccgcttta acgtcgtggt tagtggtacg ctcgatggtt
     4081 ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa ttgcatcaag gtaaccgatt
     4141 gcgtcggcag caaaagcagg aacttccttg atcgctgcgt gcgcggccag tttttgcagc
     4201 caacgtactt caacttgtac acggaatttc agcaaaccat attcgctgaa aatcccgcgc
     4261 agcgcgctga gtttatcgcc gtagcgtcca tcgacagggg aaacggcggt cagtgaggat
     4321 aattccatag atcacaactc cggggttaaa tgagcaagaa tttgttttgc ctgagtggtc
     4381 aggcgattac gagaaaacat cagttgtaga cgtccgccgc cgacctggtg ccagagcacg
     4441 gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg cctgcacttg tgggctttgc
     4501 agtacagcag gggaaccggt gacctgaatg cgcgggccaa gcgggctgat cacatcaaca
     4561 tagatagcag ccatcgcgct catcagcgtt tcggactgta aatcgaagtg ttcgagctgg
     4621 cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg cgcctttcgc tgaggagagt
     4681 ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta attcggcgtt taagccctgg
     4741 caactgctgg cattgagcac gccgagcagg gtttccagcc cgacgcgcag gttggcttcg
     4801 ctaccgccaa aaaccgccag cgtcgagctg gggttcatat caataatact gttgagtgag
     4861 acgtgtagcg catcggcatc acaatgcccc tggtgagcga gttgttgcac caggcgtgcc
     4921 gactgacaaa taccggccag ggcgagggtg atgtcatagt aattctttgc cacgttcact
     4981 gcttccttgt tttaagtaaa gataataatc agaccggcag cggcagacgc tgctcaataa
     5041 taccgccacc gaggcacact tcaccgttat agaagacggc agactggccc ggcgtcacgg
     5101 cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc gtccagcgcc ttgacggtgc
     5161 acggaatgtc ggtctggcga tagcgggttt ttaccgtgca acgcatagtg ccggtgaatg
     5221 gttcgcgatc gacccagtgc agctgctggg caatcaaccc gacagacatc agccgcgggt
     5281 gttcatggcc ctgagcgaca accagaatgt tgttttcgac gtctttgtcc accacatacc
     5341 acggttcttc ggtgccttct ttggtgccac cgatacccag acctttacgc tgaccgagag
     5401 tgtgatacat cagcccctgg tgctcaccaa tttcatggcc atcgacggta atgattttgc
     5461 ccggttgcgc cgggagataa cggcccagga actcgcggaa tttacgttcg ccaatgaagc
     5521 aaatgccggt agagtctttt ttcttcgcgg tgaccagacc aagatcttca gcaatcttac
     5581 gcacctgcgg tttttccagt tcgccgaccg ggaacaggct ttgcgcaatc tgctcatggc
     5641 tgagcgtata aaggaagtag ctctggtctt tattgctgtc cagaccacgc agcaggcggc
     5701 tcttgccatc gacatcggcc cgacgtacgt aatgaccggt agcgatataa tcggcaccta
     5761 aatcttcggc ggcaaattcg aggaaggctt taaatttgat ctccttgttg cacagaatat
     5821 ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa cagttcgaag acgttgtccc
     5881 agtactcggc agcaaagtta acggtgtgca gttcaatgcc gagcttgtcg cagaccgcct
     5941 gggcatcagc cagatccgct gccgctgtgc aatattcctc accgtcgtct tcttcccagt
     6001 tcttcataaa caggccttcg acctgatatc cctgttgttg caacagccaa gcagaaacgg
     6061 aggaatcgac accgccggac atgccgacga ttactttttt tgcggtttca gacattggat
     6121 cactcacgac attgaacttc aaggcggcgt attctatcac gcagcctttt agatgacacc
     6181 ctttgtaaaa ggccagttaa aatcgccaat catctccagc ggataacgtt gcccgctttg
     6241 ataacaacga atactttccg ccaccagcgg cgaacgaaga tttgacgcct gtaaaatttc
     6301 ttcggcgctg acccaacggc agcagtcgat atcgctgtca tgaggctggg tcgggcatat
     6361 ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc gttttatctg gcgcaatcca
     6421 ctgatgcata cgaataaagt gttgcggctg cgcgctgatg ccggtttctt cccacagttc
     6481 acgggcggcg gcttccacta aggtttcatc ggcttccaga tgcccggcag gttggttcca
     6541 taacgcttta ccattaatcg tctcttcaac gactaaaaat ttgccttctg cgtgcaccac
     6601 gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct taatctgtca cttctcgcca
     6661 ttcgccattg gcaagattat ccaaagagta atctcccatc gcatagcgaa tcagtcgcag
     6721 cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg cgattacgtc cttcatataa
     6781 ggtgatcttc agccagctgg tgggaatact tttgcgttca cgaattggtg gattccgcgg
     6841 ccataaccac gcgggttcgt caaccagctc cgcgcctgcg ggcagggtag ggccatcatt
     6901 taaggttacg ccattgcgca aggcttcaag tgcgtcttgt gtgggaatac cttccacctg
     6961 cacataatag atttttccgg tgcgtttacc cggctgggtt aaacgcgcct gcaacgcgcc
     7021 gttattggtc agcaccagca acccttcgct atcgcggtca aggcgacctg ctgcataaac
     7081 gccctgaacc gggatgaatt cttttaatgt tttgcgtccg gcttcatcgg tgaactgcgg
     7141 aagaacatcg tagggtttat tgaagaggat cacacgcgtg ggctggtttt catgtttacg
     7201 cctggtagaa cgttgcgagc tgaatcgctt aacctggtga tttctaaaag aagttttttg
     7261 catggtattt tcagagatta tgaattgcct cattatagcc taataacgcg catctttcat
     7321 gacggcaaac aatagggtag tattgacaag tcaattacaa atcattaaca aaaaattgct
     7381 ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac gtggtggcag acgagcaaac
     7441 cagtagcgct cgaaggagag gtgaatggaa agtaaagtag ttgttccggc acaaggcaag
     7501 aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa atccgattat cccttacatt
     7561 gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc tgaaagtggt cgacgctgca
     7621 gtcgagaaag cctataaagg cgagcgtaaa atctcctgga tggaaattta caccggtgaa
     7681 aaatccacac aggtttatgg tcaggatgtc tggctgcctg ctgaaaccct tgatctgatt
     7741 cgtgaatatc gagttgccat taaaggcccg ctgaccactc cggttggtgg cggtattcgt
     7801 tctctcaacg ttgccctgcg tcaggaactg gatctctaca tctgcctgcg tccggtacgt
     7861 tactatcagg gcactccaag cccggttaaa caccctgaac tgaccgatat ggttatcttc
     7921 cgtgaaaact cggaagacat ttatgcgggt atcgaatgga aagctgactc tgccgacgcc
     7981 gagaaagtga ttaaattcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa
     8041 cattgcggta tcggtattaa gccgtgttct gaagaaggca ccaaacgtct ggttcgtgca
     8101 gcgatcgaat acgcaattgc taacgatcgt gactctgtga ccctggtgca caaaggcaac
     8161 atcatgaagt tcaccgaagg cgcgtttaaa gactggggct accagctggc gcgtgaagaa
     8221 tttggcggtg aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaataccggc
     8281 aaagagatcg tcataaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt
     8341 ccggctgaat atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgct
     8401 ctggcagcgc aggttggcgg tatcggtatc gcccctggag caaacatcgg tgacgaatgc
     8461 gccctgtttg aagccaccca cggtactgcg ccgaaatacg ccggtcagga caaagtaaac
     8521 cctggctcta ttattctctc cgctgagatg atgttacgcc atatgggctg gactgaagcc
     8581 gcagacctga ttgttaaagg tatggaaggc gcgatcaacg ccaagaccgt aacctatgac
     8641 ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgatgcgatc
     8701 atcaagaata tgtaatcact acatgtgtta aatatttaaa cgggcgtata acacgcccgt
     8761 tgttttattt atgtggatat tattaatagc atatcgagca tatttatata aaacctatta
     8821 cttgagccca tatgggcata tttttataat acaactctta tgcaaacatt tatttgttat
     8881 ttcgctttct cctggaggac actcttgact gcttttgagt aaactccata aattcttgtt
     8941 gaatggtgca atgttataaa tagtaatagg atattctttt tccttaagga taataccaag
     9001 cttaaccggt gtaaatatcc ttccaggagg aagaaatata gtagattgat accagatgat
     9061 cattttcata tcaccccata tggctgaaaa agatatgccg catgtaggtt gaattaccgt
     9121 gtcaattact acccacttca tttgttatgt cttatcccac ggtatttaat atagttcatt
     9181 tggatgttca tttctttatt ttgcatatga gtatattacc ccttcaaaaa ataaattaat
     9241 taaaacgatt gcttatataa aacaaaattt aaatcaagga atctcaatgg atgttaaaca
     9301 aaatgagatt tagtgaaaac aataaattat tcacttcgtt ttagatttgt ttagctataa
     9361 tgttatacat tcaaatgact gaacatcctg taattaaaac atagccttta tgctacttgg
     9421 tgccaatttg ctaaacatta tggttgcctt tttatataac gataataatg aatataaaca
     9481 tgacatgaga ataaggtttc aatttttgag ttatatagga aaggtttaac ctgttcctgg
     9541 ctaaaataca tataactgga tgatgactaa accaaaacac atatgcgtta agtattgaaa
     9601 cgggcgtatg gcacacccgt tgtttttata aatatattaa ccgttataaa ataacgtata
     9661 aaaagtcaag tgatcacatt tcaaatatca atttgatagt atgggcttgg tgattattta
     9721 tgggtagcaa taaaaggaca gtatttatca tccataggga tagtctctgt acttttattc
     9781 ccattatgct aatgccttac tgaattatga agcatttctt aagcatccaa ctttagctag
     9841 attaatggtt tattattttc tacatcttca atatataaaa gcgtattatc aatggcgtag
     9901 taactgcgtt tgttatgatt aacatcagta acccaacgga aaacgcccgc gcctgctagt
     9961 gttgaacagt attcccgaaa tgtagatttt ccgcaaatat gaagcaatgc ggcctctttt
    10021 attttagcag gattcttggt cgtactaact tttaactggt tcctggttcc tcttaataac
    10081 aaaaccgtgt catcgtgagt aataattctg atgttatccg tagccagata ataaatgtaa
    10141 tgtgcaatac ggtgatgttt taattctgaa taaaaccagg agaagttttg ctcttttctc
    10201 acttgctcaa acatcttttg aaaaacaacg acctgatcca tcaggataat aacctcttgt
    10261 tagttgtgag actgcgtagt gtgcacgatc ggttttacca cttcaatctg gtctgtcctt
    10321 tggctgtgat atgtacagag tgtgatagag ggattacctg aattctcccg gtgagcattt
    10381 tgcaacggac cagctccggt acaaatgctg ttgtgggttc agattataac attctgtcta
    10441 aggggcggga taaaggtgaa attagggggc atgaaagatg actttataac cttgctcacc
    10501 ccaatgttgt aaaagttcgt tttgccttct cgttggtgcc atgcctgtcc agacaatcaa
    10561 tgtttgcgtc gggaacagtt ccgggcgcgg cgattcaatg ggatcagcaa gaacagaaat
    10621 gtgccatcct gacaatgata aacgccatgc ttccagccac aatcgtgctc tctcttcaac
    10681 attccacgcc atcagaatgg cttctttacc gggcttacgg cgcgtttcga aaagcgaggt
    10741 tgctgcgtat tcaattaatg cgccgtcaaa catactgctc ataatgcggg aggtgttgtg
    10801 atcaagcacg agacgctggc gaacaggaag gtaaacatga ttaatcaatt gatctactgg
    10861 gtactctcga cccagtgaaa taattctcgc gcgtagtttg gcaggattag ccatacgaag
    10921 aattgacatc atctcttctt gcaggcggct ccagtcatct tccgtatcct ggctggtggt
    10981 ttccagtaat gctttaactt tgcctacagg gacgccatta ctgatccaac gcttgatctc
    11041 ttcgatgcgt tgtatgtctt cttcatcaaa gagtcggtgt ccgccttcac tgcgctgtgg
    11101 ttttaacaaa ccgtagtggc gttgccaggc ccggagagtg acaggattaa tcccgcaacg
    11161 ttcagcaaca tcaccaatgc tgtaataagc cacaattcct ccttgcggtc acaaatctcc
    11221 gtcgccggta cacgacccaa taatactttg tacaatatac gctaaaattg tacaaagtat
    11281 aaataagatt tcagctaaat tggatgaaac attattttta atgtagatta aatttaaaca
    11341 taacgtattc attttcacga tgatttactg aaatcatgtg aaagaatgtg ctgaaaatta
    11401 ttttttctct ggccacgcta tggaagggat accattcaat ttagctttag caaacagatc
    11461 tccctgaaac atctcaattc ctgcggattc aagccacatc cactcttctg gtgttgccac
    11521 gcccatagca ctgacttgaa tttcaagtga tgtacagcat tttatgatcg cttgaataat
    11581 tgcctgccgt ggcccgcttt tatgaacatt ggtaatcaat tcctgactga ttttaattct
    11641 gtcaggctgg aagcgtgaca ggagtaacaa accggcaaaa cctgcgccaa aatgatcaat
    11701 tgctacactg ataccggcag ccttaagcga tttaatcgct tctgcaaact catcaaaccg
    11761 agatataact tcactttcag taaattcaac gatgatttgt tcaggcacca gagcattggc
    11821 ctttatttca tcaagtaaaa aagagactgc gtcaggttcg ttaaccaggg tcataggtaa
    11881 tagattgatt gaaatcattt tatcaccgag ctcaagtgcg tgtgtcatcg cgaatgcaag
    11941 cgccttactt ttgagatccg ctgtgtagat ttccccgtct ttacgctgcc caaccgctat
    12001 ggctgatggg ctatcttcat ttttttgcac aatggcttca aaagcgatta tccgccgcga
    12061 taagggatcg acaataggat gaaaggcaaa gtggtcgttt atagttggtg aaagggtaca
    12121 ggaatcaagt tctttatcag atccgtcagc gataaaaagc caggagtctt cggcagggat
    12181 ctcgaaatag gttgattgtt cggttgcaag gacaaaagta cgaaaaaatt gtagcgctct
    12241 gtcatcataa gttagctgaa attttgatgt gcctttgtcg aatacggcct gtaaaacgtc
    12301 atctcgctcg tgcaggcgca aatcaaataa ttccattccc gctttgccaa aacggcgagc
    12361 aggcgcgtaa tcgcacagca gctcaacaat attatagtgc cgtggatctt ggcatatagc
    12421 ccgatatatc attttaacct gttcttccgg accttccaga agctggaaaa aatgagaacc
    12481 attaaacagt aagatccctg ttacgtcaga ctgcatgttc ctgcgatttg ctatcgaaac
    12541 catttcttcg atttttttga caggttcgtc gtcacgtata tggctacgat aaataagggt
    12601 ggtaagcatt aacaatccag ggtaatgggt gaggcgagag taagacggta acagacatat
    12661 cttcttgtgt ctttctttta ataccaaaac ataaccgttt ctttacattg ataaaaaatg
    12721 gaaaaagttg aacacgagtt ggcgaaaaat cttgtataga ttgtcagtta aatgatgcaa
    12781 tatgttttat cataacacat tgttttatat acattagtac taattgcaaa aaattaattt
    12841 atcattctgt acacatattt cgtacaagtt tgctattgtt acctcacttc acattgatta
    12901 acatttttaa cagaggcgta gcatgcatca aaattcagtg acttcagatt ctgcgggagc
    12961 aatcactcgt tactttgcaa aagctaactt gcctactcag caggaaactc tcggggaaat
    13021 tgtgactgag attttgaaag atggacgtaa tctgagtcga aagtcgcatt gtgccaaact
    13081 tctttgccga ctggaacaag cgacagggga agaggaacag aaacattata atgcactaat
    13141 tgggttgctt tttgaataat gcaacagcct gattatttaa caggttagct atgaagtcgt
    13201 tatgaagaca tctgataatg aacgtataaa atatgaaatt actggccagg cggtgctcca
    13261 gatactgcgc atgaagataa atttttcatt gcagactctt attaagcaat tactcgtaat
    13321 gaaatcggct gaggaagatg ctttccgacg cgatttaatt gacagcataa ttcgtgattt
    13381 tagcaatagt gattcaggag ggccaaaccg aagaacagcg acagctgaca ataaaagtat
    13441 gttcaatggt aagaaaataa acagaataca ttaaaatttc ataagtaaga tgagaggtta
    13501 ccatgcttga agatactaca attcataatg caataactga taaagcgtta gcaagttact
    13561 ttcgcagttc gggtaatttg ttagaagaag agtcagcagt gttagggcag gctgtcacca
    13621 atttaatgct ttcaggcgat aatgttaata ataaaaatat tatcttaagt ctgatacact
    13681 ccctggaaac aacaagtgat attctcaaag ctgatgtgat tagaaaaaca ctggaaatcg
    13741 tgttgcgata cacagctgat gatatgtaac ttctaattat taagtataag tttatagaaa
    13801 actcattcat cttatttttg tctgtcgctt tagactatac acagcataat tttattgggt
    13861 taatatttct acgaggctga ca
//
LOCUS       DAJTYI010000102        13780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYI010000102 DAJTYI010000000
VERSION     DAJTYI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            117..1370
                     /gene="kch"
                     /locus_tag="OO564_002926"
     CDS             117..1370
                     /gene="kch"
                     /locus_tag="OO564_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6653984.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1425..1598)
                     /locus_tag="OO564_002927"
     CDS             complement(1425..1598)
                     /locus_tag="OO564_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6653985.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1741..3201
                     /gene="cls"
                     /locus_tag="OO564_002928"
     CDS             1741..3201
                     /gene="cls"
                     /locus_tag="OO564_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6653986.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3236..3565
                     /locus_tag="OO564_002929"
     CDS             3236..3565
                     /locus_tag="OO564_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6653987.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3618..4622)
                     /gene="oppF"
                     /locus_tag="OO564_002930"
     CDS             complement(3618..4622)
                     /gene="oppF"
                     /locus_tag="OO564_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6653988.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4619..5632)
                     /gene="oppD"
                     /locus_tag="OO564_002931"
     CDS             complement(4619..5632)
                     /gene="oppD"
                     /locus_tag="OO564_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6653989.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5644..6552)
                     /gene="oppC"
                     /locus_tag="OO564_002932"
     CDS             complement(5644..6552)
                     /gene="oppC"
                     /locus_tag="OO564_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6653990.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6567..7487)
                     /gene="oppB"
                     /locus_tag="OO564_002933"
     CDS             complement(6567..7487)
                     /gene="oppB"
                     /locus_tag="OO564_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6653991.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7573..9204)
                     /gene="oppA"
                     /locus_tag="OO564_002934"
     CDS             complement(7573..9204)
                     /gene="oppA"
                     /locus_tag="OO564_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6653992.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9942..10589)
                     /gene="ychE"
                     /locus_tag="OO564_002935"
     CDS             complement(9942..10589)
                     /gene="ychE"
                     /locus_tag="OO564_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6653993.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11066..13741
                     /gene="adhE"
                     /locus_tag="OO564_002936"
                     /gene_synonym="adhC"
     CDS             11066..13741
                     /gene="adhE"
                     /locus_tag="OO564_002936"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6653994.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3610 a         3415 c         3217 g         3538 t
ORIGIN      
        1 ttgattactg cggataaagt catctactat tgtttaacac attcacgcgc tgcatgtatg
       61 ttcagaaaat gtgcacgcta agataattaa acaaaattgt ggttacagag gtatcagtga
      121 gtcactgggc tacattcaaa caaacagcaa caaatttatg ggtgacccta cggcacgaca
      181 ttctcgcgct ggccgtcttt ttaaatggat tgcttatttt taaaacaatc tatggtatgt
      241 cggtcaattt gcttgatatt ttccatatca aagcattttc agagttggat ctctccttgc
      301 tggcaaacgc cccactattt atgctcggcg tctttcttgt cctgaactcc attggcttac
      361 tgttccgggc aaagctcgca tgggcaatca gtatcatttt gttgttgata gcgctaattt
      421 acaccctgca tttttatccc tggctgaaat ttagtattgg attttgcatt tttacgctgg
      481 tgtttttgct gatactgcgc aaagacttct cccacagtag cgccgcagcc gggacaattt
      541 ttgcatttat tagtttcacg acattactgt tttactcaac ctacggtgca ctttatttaa
      601 gcgaaggttt taatccgcga atagaaagtt tgatgaccgc gttctatttt tcgatagaaa
      661 ccatgtcaac cgtcggctat ggagatattg tccctgtttc tgaatcagca cgattgttca
      721 ctatttcggt cattatttcc ggcattaccg tttttgccac atctatgacc tcaatttttg
      781 gtccgcttat ccgcggggga ttcaacaaac ttttaaaagg aaacaatcat acaatgcatc
      841 gtaaagatca ttttattgtt tgcggacatt cgattctcgc catcaatacg atcctgcaac
      901 tgaatcaacg cggacaaaac gtaacggtta tcagcaactt gcctgaagat gatatcaagc
      961 aacttgagca acgcttaggc gataacgctg atgttatccc cggtgacagt aatgacagtt
     1021 cagtattaaa gaaagcggga atcgatcgtt gccgggccat tctggcgctg agtgataacg
     1081 atgcagataa cgcgtttgtt gtactctcgg caaaagatat gagcagtgat gtcaaaacag
     1141 ttctcgccgt cagtgatagc aaaaacctga ataagattaa gatggtacat ccggatatca
     1201 ttctctcacc gcaactgttt ggcagcgaaa ttctggcgcg agttttaaat ggtgaagaga
     1261 ttaataatga tatgctcgtt tcaatgttgt tgaactcagg tcatggtatt ttcagcgata
     1321 acgatgaaca agaaacgaaa gctgacagta aagaatcggc gcaaaaatag agtttaacga
     1381 cggaagagta aacttattgg gcaccgcaat ggtgcccagt acatttagat attgtagatc
     1441 gcaaacaata acgagttacg ctgtttgttt agaatgcact tcctgatgct gtggatacgc
     1501 atatttcggc gcgattgccc ctcaagccaa cgcgatttac gtcggctagc ctgacgctgc
     1561 atgcgccagc gccccacttc cgttctactc cgcttcatgt taactactct atgcaataac
     1621 agaatggtca ttataccccc gcccccgacg ctgaccagtg cttttcccgc gtttttattt
     1681 gccagatgaa tccatatgcg taaactcata acaatgcgct ttcaaaagga tttctaatct
     1741 atgacaaccg tttatacgtt ggtgagttgg ttggccattc tgggatactg gttgctcatt
     1801 gcaggcgtaa ctttacgcat tctaatgaaa cgacgcgcag ttccctccgc gatggcctgg
     1861 ctgttgatta tttacattct gccgttagtc ggaattattg cctatcttgc cgttggcgag
     1921 ctccatttag gcaaacgccg cgctgagcgc gccagagcga tgtggccttc caccgcaaaa
     1981 tggcttaacg accttaaagc ctgtaagcat atcttcgccg aagaaaatag cagtgtcgct
     2041 gcgccattat tcaagctttg cgagcgtcgt caggggatcg ctggggtcaa agggaatcag
     2101 ctacaactga tgaccgagtc agatgatgtg atgcaggcgt taatccgcga catccagctc
     2161 gcgcgccata atattgagat ggtgttttat atctggcagc ccggcggcat ggcggaccag
     2221 gtggctgaat cattaatggc ggctgcacgg cgtggtattc attgccgatt gatgctcgac
     2281 tccgccggga gtgtggcttt tttccgcagc ccgtggcccg agctaatgcg taatgccggt
     2341 attgaagtgg tcgaagcctt aaaggtcaat ctgatgcgtg tgtttttacg ccgtatggac
     2401 ctgcgccaac atcgcaagat gatcatgatc gataattaca tcgcgtacac cggcagcatg
     2461 aatatggtcg atcctcgcta cttcaaacaa gatgcgggcg tagggcaatg gattgatctg
     2521 atggcgcgta tggaaggccc catcgccacc gcgatgggga ttatttattc ctgcgactgg
     2581 gagattgaaa ccggaaaacg tattctgccg ccaccaccag atgtcaatat tatgccgttt
     2641 gaacaggcca gtggtcacac cattcacaca attgcttctg gccccggctt tccggaagat
     2701 ctcattcacc aggcattatt gactgcggct tattcggcgc atgaatattt gatcatgacc
     2761 acgccctact ttgtgccaag cgatgattta cttcatgcga tttgcacggc ggcgcagcgc
     2821 ggggtggatg tcagtattat ccttccgcga aaaaatgact cgatgctggt cggctgggcc
     2881 agtcgcgcat tctttacgga actgcttgct gctggggtta aaatttatca gtttgaaggc
     2941 gggttactgc ataccaagag cgtgctggtc gatggcgaac taagtctggt tggcaccgtt
     3001 aaccttgata tgcgtagtct gtggctgaat ttcgagatta ccctggcaat cgacgataaa
     3061 ggttttggtg cagaccttgc cgccgttcag gacgattata tttcgcgttc acgtctgctc
     3121 gatgcccgtt tatggctaaa acgtccatta tggcaacgtg tcgccgagcg actgttttac
     3181 ttcttcagcc cgttgctgta aaacgtcgcc aacagaggtt aaacaggtag tgactatgga
     3241 tatggatcta aacaatcgcc tgactgaaga tgaaacgctt aagcaggcct acgatatttt
     3301 tctcgaactg gctgccgaca atctcgatcc agctgatgtt ctgctgttca atcttcagtt
     3361 tgaagagcgc ggcggcgcgg agttattcga tccggcggaa gactggcagg aacatgttga
     3421 tttcgatttg aacccggact tttttgccga agtggtgatt ggtctggcgg acagtgaaga
     3481 tggtgaaatc aacgatgttt tcgcccgcat tctgttatgc cgcgaaaaag atcacaaact
     3541 ttgccatatc atctggcggg aataaataat cgcttttagc gtgaaaaagg gctgacaact
     3601 gtcagccctt attgttttta aagcggatcg actttcaggc aagaaacggc gtgtctgaaa
     3661 ctcccctcca gaacaggacg tgttttggcg cactccggac cggcaatcgg gcaacgggta
     3721 cggaaaacac aaccggaagg cgggttgatc ggcgacggta attccccttc cagtaactgg
     3781 atggttttgt tcttctccag atccggatca ggtatgggga ctgccgacat caatgccctg
     3841 gtgtaaggat gtagtggatt gtggtagacc tcatcatagg tccccagttc tactgcatgg
     3901 ccgagataca tcaccaacac acgatcggaa atgtgtttta ccacggccag gtcatgagca
     3961 ataaaaatta atgacaatcc catctcacgt tgcagctgct ggagcaggtt gaccacctgc
     4021 gcctgaattg acacgtccag cgccgaaacc ggctcatcgc agataatcag cttcggttca
     4081 agaataagag cacgagcaat cccgatacgc tggcactgcc caccggagaa ctcatgcgga
     4141 tagcggttaa tcaggttagg caataacccg actttcagca tcatcgcctt cacgcgctcg
     4201 cgaacttcct ggcgtgacat tttcggatga taagtacgca gtggttcagc gatgatctcg
     4261 ccgatggtca tacgcgggtt tagcgatgcc aacggatcct ggaaaatcat ctgaatatca
     4321 ctgcgaacgg cacgccattc atcgggcttc atgcccagca actctttacc taaccaggca
     4381 acatgaccgt cggtcgcctt gaccaaaccg atgatggcgc gagcaaaggt ggacttaccg
     4441 catcccgatt cccctaccac acctaatgtt tccccttcat acaggcgaag agtgacacca
     4501 tcgacggctt tgagcgtttt cggcggttgc cagaaccact gtttgccatc tttgatttca
     4561 aagtgcactt taagatcggc gatttcaagg aggacttttc ttccttcagt tacagcattc
     4621 ataacagttc ctccaccggt ttaaagcaag cacgcagacg gccaggcgta aactcttcca
     4681 gcggcggtgc gctactacaa atttccatcg catgtggaca acgtggctgg aacgggcaac
     4741 ctttcggtaa tcgcagcaag tttggcggat tacccgggat ggtcaacatt gtttcacctt
     4801 ccgcatcaag acgcggcacc gcgttgagca aaccgataga ataaggatga acgggttgat
     4861 aaaagacatc gcgcgcattg ccatattcca tcgtgcgccc ggcgtacatt accagcactt
     4921 tgtcacagat ccccgccacc acgccaagat cgtgggtgat cataatgatg gcagtattaa
     4981 attcccgctt cagttcattc aatagcgtca tgatctgcgc ctgtacggtg acgtccagcg
     5041 ctgtagtggg ttcatccgca atcagcaact taggtcgaca tagcaatgcc atcgcaatca
     5101 tgactcgctg acgcatgccg ccagaaaatt cgtgcgggta cattttcatg cgtttacgtg
     5161 cttccggcat ttttaccgca tcgagcatcc gcaccgactc ttcaaacgcc tcagctttgc
     5221 tcatgttctt atgcagcatc agcacttcca tcaactgctc accgacgcgc atatagggat
     5281 tcaacgaagt cattgggtcc tgaaaaatca ttgagatttg ttcagcgcgc agtttattga
     5341 gttcacgctc tggcaaattg aggatttcac gcccattgaa ggttgccgct ccgccaatgc
     5401 gtccgttggc agccaacagg cccatcaacg caaatgcagt ttgcgattta cccgaaccag
     5461 actcacctac aatgcccagc gtttctccgg cacgtaggga aaaatttaaa tcattgaccg
     5521 ccgtgacgtc gccgtccggg gtactaaagg tgacacgcaa atctttcacg ttcagcagtg
     5581 cgtcagcctg ttgttgtgcg agcggcacag ttgcagtttc aattacgctc atggctgcac
     5641 tccttaacga tctttcgggt cgagggcatc acgcaagcca tcgccgataa agttgaaaca
     5701 aaacagtgtc accacaagga atcccgctgg gaacaacagt aaccatggag agacttccat
     5761 cgagttcgcg ccatcactca gcaatgcccc ccagctgctt aacggctctt gcgtacccaa
     5821 ccccaggaag ctaaggaaag attcaaagag gatcatgctg ggcaccagta gcgatgcgta
     5881 gaccaccacc acaccgagta cgttcggcac aatgtggcga ataacaatgc ccggcgtcga
     5941 tacaccgcca acttgtgccg cctcaataaa ctctttgcgc ttcagactca gggtttgccc
     6001 acgcacaata cgagccatat ccagccagga aaccatgcca atcgccacga aaatcagcag
     6061 gatgttttgg ccgaaaaagg tcaccagcaa aatgacgaag aacatgaatg ggaaggagtt
     6121 gaggatttcc agcagacgca tcattaccga atccacttta ccgcccagat aaccggaaag
     6181 cgaaccataa agtgtcccca cgaccactgc caccagtgcc gcagcaacac cgaccatgag
     6241 tgagatacgc ccgccaatcg caacgcgcac aagcaggtcg cgaccggatg agtcagtacc
     6301 aaagtagtga ccggactcca tatccggggc gctggacatc atcgcccagt cagtatcgtc
     6361 ataggcaaac tgggaaagca tcggtgccag gattacaaat aacgcgatca gcaccagcac
     6421 tatcagactg gcaaccgccg cacggttatg cataaaacgt cgacgtgcgt cctgccacaa
     6481 gctgcgccct tcgacctcca gcttttcact gaaattttcc agcgtctcgc tgtttttctt
     6541 acttaacatc atcgcgagct ccagtatcag taacggattt tcgggtcgat aaccgcatat
     6601 agcacatcga caatggcatt aaacaaaatg gttaacgcac caaccaggat ggtcaggctt
     6661 aacactaagg aatagtcacg gttcaatgcg ccattaacga acagttgccc aatccccggc
     6721 aaaccataaa tggtttcgat gaccatagaa ccggtaataa tgccgacaaa tgcggggccc
     6781 atataggaga gcacgggtaa cagagcaggt tttaatgcgt ggcgtaaaat gatccgccgc
     6841 ataggtaacc ctttcgcccg ggcagtacga ataaagttgg agtgtaatac ttcaatcata
     6901 gagccacggg taatacgcgc aatactggcg atataggcga gtgacaacgc caccatcggc
     6961 aatatcatga atttaagcgc cccaccattc cagccaccgc ccggcagcca atgcaaaatg
     7021 atcgcaaata tcatgactaa taatggcgca accacaaaac tggggataac aaccccggtc
     7081 attgccagcc ccatcacggt atagtcccat ttggtgtttt gttttaatgc ggcaataacg
     7141 ccagcactaa cacccagtat taccgcaagg aaaaatgcgg cggctcccag tttggcagaa
     7201 acgggaaaac tggatgccac caggtcattg accgaataat ctttatattt aaacgatgga
     7261 ccgaaatcgc catgcgccag ttgtttcagg tagctgaaat actgtgtcat gatcggatca
     7321 ttaagatgat atttcgcttc gatattggcc atcacttctg gcggtaaagt acgttcgccg
     7381 gtaaaagggc ttcccggcgc gaggcgcatc ataaagaacg aaatagtaat aagaataaat
     7441 agcgtcggaa tcgcttccag acagcgacgt agaataaatt ttaacattgc cctaccttct
     7501 ggcctgtgcc ttctgtaatg cgataaaaat cagacaccgt ggagcaggac actcctgccc
     7561 cacgtattgc cattagtgct tcacaatgta catattccgg gtataggtat tatccagcgg
     7621 atctttgccg gtatagccac caacccacgg tttcaccaga cgcgcattca cgtagtaata
     7681 aacaggaaca atggccgaat ccttatccag ctgttgttct gctttagtgt acagagctgt
     7741 gcgctgcgcc tcgtcagtca ctttcagcgt ttccgccata atgctgtcaa aggccgggct
     7801 cttataatgc gcggtattca tcgagctgtt cgacagcatg gtgttcagga aggaagttgg
     7861 ttcgttgtag tctgcacacc agcctgcacg ggccacatca aaagtgccct ggtgacgggt
     7921 gtcgaggaac gttttccact cctggttaac cagtttgacg tttacaccaa tgtttttctt
     7981 ccacaatgaa gaggcagcaa tcgccagctt tttatgcaga tcggaggtgt tataccgcag
     8041 gttgatggtc aacggtttgt ctgcggtata acccgcttca gccagcagtt ttttcgcttc
     8101 ttcgttacgt ttttcctggc tccagccaaa ccattctggc tgagtcaatt ttgcgccatc
     8161 agtatacggt ggagtgtaac cataggcggg catgtcgccc tgcgctttca ctttattaac
     8221 aatgatatcg cggtccatac ctagtttcag tgcggtacgc acacgcacat cgttgaatgg
     8281 cggtttctgg ttgttaattt cgtaatagta agtgcacagg tatggatcaa cgtgaacttc
     8341 gtccgggatc tcttttttca gcttctggaa caattcgatc ggcatgttgt tatacgtcat
     8401 gtcgatttca ccactacggt agcggttgac atcggtaact tcagaagcaa taggcaaata
     8461 ggttacctgg ttaataacgg ttttcgcgtt gttccagtag gtcgggctgc gttcaagaac
     8521 gattcgttcg tttacgaccc aatcttttaa ggtataggca ccgttggtga cgatattacc
     8581 aggctgggtc catttttcgc cgaatttctc gatagcggct tttggcaccg gtgaagttga
     8641 tgggtgaaca agtaatttat agaagtacgg aacgggttca cttaaggtga cttctaatgt
     8701 gtgatcatca atagctttca cgccgagatc ggtaatcggt tttttccctt caagaatttc
     8761 atcaataccg gcgatatgcc catattgcag ataactggca tacggagaag cagtgttcgg
     8821 atcaacagaa cgttgccagc tatacacaaa gtcttgtgct gtgactggcg tgccatcaga
     8881 ccatttcgca tctttacgca aatggaaggt ccagactttc gcgtctttat tatcccagga
     8941 ttcagcgacg ccaggtgctg gatgaccgtc aagatcgctg accagtaagc cttcaaacag
     9001 gtctcggctg atattagact ccggaacacc ttcaattttg tgcggatcta atgactgaac
     9061 ttctgaacca ttgttacgta ccagtgtttg tttttccgcc agtgtgacgc ctgcgggtac
     9121 atcagctgcc agcgcgacat tccctgccat tagcgcagcc agaacgccag ctgctactaa
     9181 acttctcttg gtgatgttgg tcattgtttt ttggactccc tcattataat tactggtatt
     9241 aaccagcatg tgtaatcccg gattgggacc ctgcacaggc tcggagactt tctgctgtca
     9301 ggttacttta tcgttacgta ttactatcac cgactttatt tattctggtg attcagacga
     9361 tcaccttatg tcgattcgta ctgtgtctcc cctgtcgaga cacattattc tttcgcgacg
     9421 ctctttaatt gttaattatt ctcattacca ataaagaatc gtctggcggt tgcccggaaa
     9481 gtaccaaatg cggttcttac tcgccaatac attttgcaaa attgttaagc gattctcttt
     9541 tattgaccgt atcagaatct atagcttgca tccatgcctt aacaaacatc tttcaattca
     9601 atcaaataga gattggctaa gcatgtctat cttttctgat tacagttcca gtagtgaaat
     9661 gcacaataat ttaacaattg attattattt agcactttcc tctaccaaag gtagtggaat
     9721 tactaatatc atttcaatta ttcttcagca agcccaggat tatgatgtgg cgaaaataac
     9781 atgatggtga ggagaagcag ttaaggaaat ccttataact aactgtaatt tgagaagaat
     9841 atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg
     9901 aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg
     9961 aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc
    10021 ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc
    10081 atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac
    10141 agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc
    10201 cccgccatca acggcaacgc caatggcacc acaccaatac tttcacgtac cgcggtttct
    10261 gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct
    10321 atcgtcacca ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt
    10381 tgtagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg
    10441 gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag
    10501 acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa
    10561 acgggaaaat caaaaagggt ctgaatcacg gttagctccg aagcaaaagc cggataatgt
    10621 tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac
    10681 ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct
    10741 aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaagcga
    10801 tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag
    10861 tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt
    10921 aaactctttt agtaaatcac agtgagtgtg agcgcgaata agcttttgat tttcatcggt
    10981 taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa
    11041 agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact
    11101 cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga
    11161 caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat
    11221 ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc
    11281 ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga
    11341 cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc
    11401 gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagcctga agacccgtaa
    11461 cgccattatc ttctccccgc acccgcgtgc aaaagatgca accaacaaag cggctgatat
    11521 cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca
    11581 accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc
    11641 gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt
    11701 aggcgcgggt aacactccag ttgttattga tgaaactgct gatatcaaac gtgcagttgc
    11761 atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt
    11821 tgttgttgtt gactctgttt atgacgcagt acgtgaacgt ttcgcaaccc acggcggcta
    11881 tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa atggtgcgct
    11941 gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt
    12001 accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt
    12061 cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc
    12121 ggtagaaaaa gcagagaaac tggttgctat gggcggtatc ggtcatacct cttgcctgta
    12181 cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacagc
    12241 tcgtatcctg attaacaccc cggcttctca gggtggtatc ggtgacctgt ataacttcaa
    12301 actcgcacct tccctgactc tgggttgtgg ttcctggggt ggtaactcca tctctgaaaa
    12361 cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt
    12421 gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga
    12481 tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa
    12541 caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt
    12601 cttcttcgaa gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg cagaactggc
    12661 aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc
    12721 gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg
    12781 ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat
    12841 gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac
    12901 tgacgacgct actggtcaga aatacccgct ggcggactat gcgctgaccc cggatatggc
    12961 gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct
    13021 ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga
    13081 tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga
    13141 agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcaacta tcgcgggtat
    13201 cgcatttgcg aacgccttcc tgggggtatg tcactcaatg gcgcacaaac tgggttccca
    13261 gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa
    13321 tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg
    13381 ccgtcgttat gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc
    13441 tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc
    13501 gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact
    13561 gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc
    13621 cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac
    13681 tgcagcgaaa aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta
    13741 atcagtagcg ctgtctggca atataaacgg ccccttctgg
//
LOCUS       DAJTYI010000103        13712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYI010000103 DAJTYI010000000
VERSION     DAJTYI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            24..425
                     /locus_tag="OO564_002937"
     CDS             24..425
                     /locus_tag="OO564_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6653995.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            437..808
                     /locus_tag="OO564_002938"
     CDS             437..808
                     /locus_tag="OO564_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6653996.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            795..1385
                     /locus_tag="OO564_002939"
     CDS             795..1385
                     /locus_tag="OO564_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6653997.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPMSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1382..1781
                     /locus_tag="OO564_002940"
                     /pseudo
     CDS             1382..1781
                     /locus_tag="OO564_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1792..2532
                     /locus_tag="OO564_002941"
     CDS             1792..2532
                     /locus_tag="OO564_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6653998.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2591..2977
                     /locus_tag="OO564_002942"
     CDS             2591..2977
                     /locus_tag="OO564_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6653999.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2986..3315
                     /locus_tag="OO564_002943"
     CDS             2986..3315
                     /locus_tag="OO564_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6654000.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3287..6226
                     /locus_tag="OO564_002944"
                     /pseudo
     CDS             3287..6226
                     /locus_tag="OO564_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6226..6555
                     /locus_tag="OO564_002945"
     CDS             6226..6555
                     /locus_tag="OO564_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6654001.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6555..7253
                     /locus_tag="OO564_002946"
     CDS             6555..7253
                     /locus_tag="OO564_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6654002.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7258..8001
                     /locus_tag="OO564_002947"
     CDS             7258..8001
                     /locus_tag="OO564_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6654003.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7938..8539
                     /locus_tag="OO564_002948"
                     /pseudo
     CDS             7938..8539
                     /locus_tag="OO564_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8600..12079
                     /locus_tag="OO564_002949"
     CDS             8600..12079
                     /locus_tag="OO564_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6654004.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTERLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLIVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            12147..12746
                     /locus_tag="OO564_002950"
     CDS             12147..12746
                     /locus_tag="OO564_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6654005.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12810..>13712
                     /locus_tag="OO564_002951"
     CDS             12810..>13712
                     /locus_tag="OO564_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6654006.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPD"
BASE COUNT         3238 a         3243 c         4362 g         2869 t
ORIGIN      
        1 tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa cgtctgagag
       61 aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca gatattgcgc
      121 agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac atccgggata
      181 agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata tcagcgcagc
      241 cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg gtgacgctgc
      301 ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg ccgggaatgg
      361 cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg gccagaatgc
      421 aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac ttgatctcgc
      481 agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag gccggctaaa
      541 agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg tggccggcgg
      601 tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata ttttacggct
      661 gtgtcgcaat gattcgctga tgattagtcg tgagtctttc tgtgtggatc gtatcacccc
      721 tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg gaaacgtaag
      781 ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac agccttgata
      841 cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag aaagcggtct
      901 cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag gtgaaaggga
      961 tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg tcaggaaaaa
     1021 tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg aacacaacac
     1081 ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg gcatttgttc
     1141 agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg cgttttgcaa
     1201 cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag gtagtgaaaa
     1261 tcccgatgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc atcattgctg
     1321 cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta catctgagta
     1381 aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg aacagtgtgg
     1441 agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa ctgcctgccg
     1501 tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga gatgactggc
     1561 aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca gagctggata
     1621 tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt tccggactca
     1681 tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg gctacctggg
     1741 cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt aatgaccaca
     1801 ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta taccggcaac
     1861 ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc gatggtgaag
     1921 gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct cgatgatgaa
     1981 gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac ttcggcgacg
     2041 ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt cgattccggt
     2101 gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt ccgtggctgg
     2161 ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac tgtaaaaatc
     2221 agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg tgttaccgga
     2281 ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac actgaccttt
     2341 acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc agatcctcag
     2401 actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca gcagggcagt
     2461 gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc ggtgaccgtc
     2521 agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct ttttgcatcc
     2581 ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt ccgtggtgct
     2641 cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc agcgaaaggc
     2701 aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg cttttctgcg
     2761 tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg ctgagcagtc
     2821 tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg atgcgctggg
     2881 cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga atgccgggga
     2941 tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca gtttgtcatg
     3001 cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga tatgtcggcc
     3061 actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga tgtgtggatg
     3121 gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg cagcagcgat
     3181 gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga gcgaacggac
     3241 gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg accagatagc
     3301 caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa ttccccgtat
     3361 caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc gtatgcagcg
     3421 ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt cttcggctgc
     3481 aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc atgaacgcat
     3541 ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga aaatgcgcga
     3601 ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg ccgcctttta
     3661 tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac agcgtattca
     3721 gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt atctggcgct
     3781 gatttcggag atcacggtga aaacccgtgc cctgacgcag gcagaggaac aggccacccg
     3841 gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga atctgtcgtc
     3901 ttctgagttg cttcgcgcca gggcggctca gctgggggtg ttaatcagtc agatggcgcg
     3961 cggcaatttt ggtgcgctga ggggatccgg gataacgctg gctaaccgtg ccggatggac
     4021 aggcgcactg atgtcgccga aaggcatgat gactggcggc gttattggcg gacttgtcgc
     4081 ggcggtcctg ggtctgggta aagcctggca tgacggcgga aggagggcga ggaatttaac
     4141 cgtcagctgg cgctgacggg acattatgcc ggtgtcactg tcgggcagtt gtggaaactc
     4201 agccgggcca tatccgggaa tggtatcacg caacatgcgg cagccggtgc gctggcacag
     4261 gtggtgggca gtggggcatt tcatggaaac gatattggta tggtggcgaa agctgccgca
     4321 cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt taagcggctg
     4381 aaggatgatc ctgtaaatgc cgcgaaggcg ctggacaatg cgctgcattt tttgactgcc
     4441 actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga tgcggcccgg
     4501 atcgccatgt ctgcgctggc agaggaaacc ggtaaacgca catctgatat tgataataat
     4561 ctcaatgcgc tgggtagtac gctgcaaacc ttgtctgact ggtggaagca gttctgggat
     4621 gcggccatga atattggtcg tgaagactcg ctggatgcgc agattgatgc gttacaggaa
     4681 aaaattcagc gcgcgaaaaa atatccgtgg acaaacgcct ccacacaggt ggagtacgat
     4741 cagcagcgtc ttaacgatct tcaggagaaa aaacgccgga aggatttgca ggatgcaaaa
     4801 gcgcaggcag aacggaatta ccaggagcaa cagaaacgcc ggaatgctga aaatgccgcg
     4861 ctgaaccgga tgaatgaaac ggaagctgca cgacatcagc gggaaattgc gcgtattaat
     4921 gccatgcagt acgccgacca ggctgtcagg gatgcggcga tacagcgtga aaatgaacgt
     4981 tacgaaaaag ccattaagaa aaatacacgg gcaacccgta atgatgaggc cacccggtta
     5041 ttgctgcagt acagtcagca gcaggcacag gtggaagggt agattgctgc tgccagacag
     5101 tcagcaggca ttgccactga aaggatgact gaagcgcata aacagcttct ggccctgcag
     5161 cagcgcatca gcgacctgga cgggaaaaag ctgacggcag atgaaaagag tgtgctggcc
     5221 cgtaagaatg agctgattca ggcgctgacg ctgctggatg tgaaacagca ggagctgcag
     5281 aaacagacag cgcttaacga cctgagaaaa aaaacggttc agctgaccag ccagctggca
     5341 gacaaagaac gtgcactgcg tgagcagcac aatctggata ttgccactgc aggtatgggg
     5401 gataagcagc ggcagcgcta ccaggcacag ttgcgcatcc ggcaggaata ccggcaacag
     5461 ttgcaacagc ttgagaatga cagtcgccag aaaggcactt acgggacgga ggactaccgg
     5521 agggctgagg aggtgctgaa ggggagcctg aagcgacaac tgaatgaaaa caaacgctac
     5581 tggcaggaac tggaagtggc gcagggcgac tggaaaaacg gtgccatgcg ggcgtttcag
     5641 aattttacgg cggatgcgga taatgcggcg ggaacggcag aacagatgtt tacagtggca
     5701 ttcagcagtg ccggtaatgc actggcgaca ttctgtacca ccggtaagct gaattttaaa
     5761 tccttcacct cttccctgtt gtcagatatg gccagaatta tggcacagat ggccatgatg
     5821 caggcggtaa agggcgtcgg ttctttattc ggcttcacga ctaatgctga tggcggtgtt
     5881 taccagtctg ctgatttgag tcgctacagt ggcacggtgg ttaaccgtcc gacgtttttt
     5941 gcttttgcaa aagacgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccactg
     6001 cgtcgcggtg ctgatggtaa gctggggggt tgtggcggat actggtggtt caggcatggt
     6061 gatgtttgcc ccgcagtaca acattgagat caataacgac ggcacgaacg ggcagatagg
     6121 tccggctgcc ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg actttatgca
     6181 acagcaggcc cgtgatggtg gccggttaag tggagcatac cggtaatgga gacgtttcac
     6241 tggaaagtgc gcccggatat gaatgtggta tcagagccga aagtggtgac agtgaagctg
     6301 ggcgatggtt atgaacagcg tcgtgcggcg ggactgaata accagttgtc gacttacagc
     6361 gtgacgatac gtgttcgtaa aggtgaacac ccatctttaa aagcctttct ggaacggcac
     6421 ggtggcgtcc gcgcatttca gtggacgcca ccttatgact ggaaactgat cagggaggtt
     6481 tgtcgtaaat ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa
     6541 caggtggtga actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg
     6601 agcagtcagc ccgggtggag ctctgggaaa tcgatctgac agaggtcggt ggtgagcgtt
     6661 attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcggcagt
     6721 atcaggcata ccccattcag ggggcggggt ttgaactgaa cggcaggggc tgtgctgccc
     6781 gtccgacact gacggtctct aacctgcacg gcatggtcac cgggatggcg gaagatctgc
     6841 agagtctggt cggcggaacg gtggtccggc gtaaggttta cgaccgtttt ctggatgcgg
     6901 tgaacttcgt caacggaaac agcgacgccg atccggaaca ggaggtgatc agccgctggc
     6961 gcatcgagca gtgcagcgaa ctgagcgcgg tcagtgcctc ttttgtgctg gccacaccaa
     7021 cggagacgga tggcgcggtt ttcccggggc gtatcatgct ggcgaatacc tgtatgtgga
     7081 cttaccgttc tgatgagtgt ggttacacgg gcagggcagt ggctgacgag ttcgacaaac
     7141 caacgacgga tatccggaag gacaaatgca gcaagtgtat gcgcgggtgt gagttgcgca
     7201 acaataccgg taatttcggc ggtttccttt ccatcaataa actttctcag taaatccatg
     7261 acacagacag aatcagcgat tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgt
     7321 ggattcgtgg tgagaacgcc ggagggagac aggtatcttc ccagcgagaa tatctccggt
     7381 gagccggagg aacggttccg gatggctccg gaggactggc tgcgggcaca aatgcagggt
     7441 gagattgtgg cactggtcca cagtcatccc ggtggtctgc cctggctgag tgaggctgac
     7501 cggcggctgc aggtgcagag tgatttgccg tggtggctgg tctgccgggg ggcgattcac
     7561 aagttccgct gtgtgccgca tctcaccggg cggcgctttg agcacggggt gacggactgt
     7621 tacacgctgt tccgggatgc ttatcatctg gcggggattg agatgccgga ttttcaccgc
     7681 ggggatgact ggtggcgtca cggtcagaat ctctatctgg ataatctgga ggccacaggg
     7741 ctgtatcagg tgccgttgtc atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt
     7801 tcatcggtgc cgaatcatgc cgccatttac tgcggcgatg gtgagctgct gcaccatatt
     7861 cctgaacaac tgagtaaacg agagaggtat accgacaaat ggcagcgacg cacacactcc
     7921 ctctggcgtc accgggaatg gcacgcatct gcctttacgg ggatttgcaa cgatttggcc
     7981 gccgcatcga ccttcgtgtg aaaacggggg ctgaagccat ccgcgcactg gccacacagc
     8041 tcccggcgtt tcgtcagaaa ctgagtgacg gctggtatca ggtgcgcatt gccgggcgtg
     8101 atgcaggtga aaccgaattg tctgcccgtc ttaatgagcc gctggcaaat ggtgcagtga
     8161 tccacatagt accgcgtctg gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg
     8221 gggcagctgt tatggcggtt gctatatgga tgccggggta ggaattatgg cgagtaatct
     8281 gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcaccgaa
     8341 agccagaact ccccgtacac agacaacgga taacggcaaa cagaacacct gtttctcctc
     8401 actggataac atggttgccc agggcaatgt tctgcctgtt ctgtacggtg aaatgcgcgt
     8461 ggggtcgcgg gtggtatctc aggagatcag cacggcagac gaaggggatg gtggtcaggt
     8521 tgtggtgatt ggtcgctgat gcaaaatgtt tcatgtgaaa ccgcctgcgg gcggttttgt
     8581 cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc
     8641 gaaggacaac ctgaaatcca cgcagttgct gagtgtgatt gatgccatca gcgaagggcc
     8701 ggttgacggt ccggtggatg gattaaaaag cgtgctgctg aacggtacgc cggtcctgga
     8761 cagcgagggg aagacaaact tttccggtgt tacggtggtg ttccgcgccg gcgagcagga
     8821 gcagacaccg ccggaggggt ttgaatcttc cggctcagag actgtgctgg gtacggaagt
     8881 gaaatacgac acgccgatca cccggaccat cacgtcggca aacattgatc gtctgcgcct
     8941 gaccttcggt gtgcaggcac tggtggaaac cacctcaaag ggggaccgga atccgtcgga
     9001 agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac
     9061 cattaagggc aaaaccactt cgcagtatct ggcctcggtg gtggtgggta acctgccgcc
     9121 gcgcccgttt aatatccgga tgcgcaggat gacaccggac agcaccacag accagctgca
     9181 gaacaaaacg ctctggtcgt cgtacaccga aatcatcgat gtgaaacagt gctacccgaa
     9241 cacggcgctg gtcggcgtgc aggtggattc agagcagttc ggtaaccagc aggtgagtcg
     9301 caattatcat cttcgcgggc gcattctgca ggtgccgtcg aactataacc cgcagacgcg
     9361 gcaatacagc ggtatctggg acggaacgct taagccagca tacagcaaca acatggcctg
     9421 gtgtctgtgg gacatgctca ctcatccgcg ctacggcatg gggaaacgtc ttggtgcggc
     9481 agatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga
     9541 cggatttggc ggcacggagc cgcgcatcac ctgtaacgct tacctgacca cacagcgtaa
     9601 ggcgtgggat gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg
     9661 gcagacgctg acgttcgtgc aggaccgacc gtcggataaa gtgtggacct ataaccgcag
     9721 taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaagga
     9781 ccgccataat gtcgttgagg tgaactggat tgatccggac aacggtcatg agacggcgac
     9841 agagcttgtg gaggacacgc aggccattgt ccgttacggt cgtaacgtca cgaagatgga
     9901 tgcctttggc tgtaccagcc ggggacaggc gcaccgcgcc gggctgtggc tgattaaaac
     9961 ggaacggctg gaaacgcaga ccgtggactt cagcgtcggc gcagaagggc ttcgccatgt
    10021 accgggtgat gtcattgaaa tctgcgatga tgattatgcc ggtatcagca ccggtggtcg
    10081 cgtactggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc
    10141 gtcctccggt accacgctga taagcctggt tgacggaaat ggcaatccgg tcagcgtgga
    10201 ggttcagtcc gtcaccgacg gcgtgaaggt gaaagtgagc cgggttcctg acggcgttgc
    10261 agaatacagc gtgtgggggc tgaagctgcc gacgctgcgc cagcgcctgt tccgctgcgt
    10321 gagtatccgt gagaacgacg acggcacgta tgccatcacc gccgtgcagc atgtgccgga
    10381 aaaagaggcc atcgtggata acggggcaca ctttgacggt gaccggcgcg gcacggtgaa
    10441 tggtgtcacg ccgccagcgg tgcagcatct gaccgccgaa gtcaccgcag acagcgggga
    10501 atatcaggtg ctggagcgat gggacacgcc gaaggtggtg aagggtgtga gcttcctgct
    10561 tcgcctgacc gtggcagcgg acgacggcag tgagcggctg gtcagcacag gcaggacgac
    10621 ggaaaccaca taccgcttca ggcagctggc gctggggaat tacagtctga tagtccgggc
    10681 ggtaaatgcc cgggggcagc agggcgatcc ggcgtcggta tcgttccgga ttgcggcacc
    10741 ggcagcgcct gtcactattg aactgatacc ggggtatttt cagataacgg tggtcccgaa
    10801 actggctgta tatgacccga cggtgcagtt tgagttctgg ttttcggaaa agcggattgc
    10861 ggatatcagg caggttgaaa ccagcgcccg ctatcttggc acggcgctgt actggatagc
    10921 cgccagtatc aatatcaggc cgggccatga ttattatttt tacgttcgca gtgtgaacac
    10981 cgttggcaaa tcggcatttg tggaggccgt cggtcgggcg agcgattatg cggaaggtta
    11041 tctgagtttt tataaagggt tgatcaataa aacgcatctc ggcaaggagt tgtggacgca
    11101 gattgataac ggtcagcttg cgccggacct gactgaaatc aggacgtcca ttacgaatgt
    11161 cagcaatgaa atcacgcaaa ccgtcaataa aacactggaa aatcagagtg ctgcaatcca
    11221 gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt gggctgtgaa
    11281 gctgcaacag atgaaggacg gacgccttta tattgcgggt atcggtgccg gtattgagaa
    11341 tacgccagca ggaatgcaga gtcaggtgct gctggcggca gacaggattg cgatgattaa
    11401 tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag ggcgatcaga tattcatgaa
    11461 cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc agcggcggta atcctccggc
    11521 attttccctg acacctggcg gacggctgac ggcgaaaaat gccgatatca gcggtaacgt
    11581 gaacgcgaac tccgggacgc tcaacaacgt cacgactaat aagaactgtc gggttctggg
    11641 aaaattgtcc gcgaaccaga ttgaaggcga tctcgttaaa acagtgggca aacctttccc
    11701 acgggactcc cgggcaccgg agaggtggcc atcagggacc attaccgtca gggtttatga
    11761 cgatcagccg tttgatcggc aaattgttat tcccgcggtg gcgtttcgcg gtgctaaaca
    11821 tgagcggaag aataacaata tttattcgtc atgccgcctg atagtgaaga aaaacggtgc
    11881 tgaaatttat aaccgaacga ccctggataa tacgctgata tatacgggtg ttattgatat
    11941 gcctgccggt cacggtcaca tgacgctgga gttttctgta tcggcatggc tggtaaatgg
    12001 ctggtatccc acagcaagta tcagcgattt gctggttgtt gtgatgaaga aagccactgc
    12061 aggcatcacg attagctgaa ttttataacc cagatacggg caccagaaat ggtgcctttt
    12121 ttattgcaga aaagcgagag gtaattatgc gtaaagtttg tgcagccatt ttgtccgcag
    12181 ccatctgtct ggctgtatcc ggtgcgcctg catgggcgtc tgaacatcag tccacgctga
    12241 gcgccgggta tcttcagccc catactgata tgccaggcag cgatgacctg aagggcatta
    12301 acgtgaaata ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg
    12361 ccaacgctga agatgagcaa aaaacgcatt acagcgatac ccgctggcat gaggattccg
    12421 tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt gcgcgtgaat gaatggttca
    12481 gcgcgtatgc gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc
    12541 tccgcgtaac tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc
    12601 gccacagcaa cacgtctctg gcgtgggggg ctggtgtgca gtttaacccg accgaatccg
    12661 tggccattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca
    12721 ttgtgggtgt cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct
    12781 ggttcaggcg ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag
    12841 atggggcggg taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc
    12901 cgaccgttgt ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga
    12961 ttgaggctga gccgtggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc
    13021 gtgtcgggga tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga
    13081 atgcaccaaa ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg
    13141 cgcaggcgca gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg
    13201 tggcggcagc cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg
    13261 atgcaaccgt cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag
    13321 caaatgcagc cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag
    13381 aacaggccaa aaatgacatt gatgctgcgc tgaccggcac cctgaaaacg gctaaccatc
    13441 tgtctgaaat cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat aatctggggc
    13501 tgaaaagtgc ggccacgatg gaagcacaga gcgacattta cgaccggaca aaaggccgtc
    13561 tggcgatacc cggcgcattc ggctttgggt gtgcttttct gcctgaagat gttatccgtt
    13621 ttgacactaa gagtgatttc ctggcctggg taaggaatgc gctgccaggt gaatattccg
    13681 ttgctggccc ttacggcatc atcatacccg ac
//
LOCUS       DAJTYI010000104        13664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYI010000104 DAJTYI010000000
VERSION     DAJTYI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            117..1418
                     /gene="rstB"
                     /locus_tag="OO564_002952"
     CDS             117..1418
                     /gene="rstB"
                     /locus_tag="OO564_002952"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6654007.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1494..2423
                     /gene="tus"
                     /locus_tag="OO564_002953"
     CDS             1494..2423
                     /gene="tus"
                     /locus_tag="OO564_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6654008.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2420..3823)
                     /gene="fumC"
                     /locus_tag="OO564_002954"
     CDS             complement(2420..3823)
                     /gene="fumC"
                     /locus_tag="OO564_002954"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6654009.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3966..5612)
                     /gene="fumB"
                     /locus_tag="OO564_002955"
     CDS             complement(3966..5612)
                     /gene="fumB"
                     /locus_tag="OO564_002955"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6654010.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5811..6986
                     /gene="manA"
                     /locus_tag="OO564_002956"
     CDS             5811..6986
                     /gene="manA"
                     /locus_tag="OO564_002956"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6654011.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7087..8595
                     /locus_tag="OO564_002957"
     CDS             7087..8595
                     /locus_tag="OO564_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6654012.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8821..10086)
                     /gene="uidC"
                     /locus_tag="OO564_002958"
     CDS             complement(8821..10086)
                     /gene="uidC"
                     /locus_tag="OO564_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6654013.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10125..10292)
                     /locus_tag="OO564_002959"
     CDS             complement(10125..10292)
                     /locus_tag="OO564_002959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6654014.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10308..11498)
                     /gene="uidB"
                     /locus_tag="OO564_002960"
     CDS             complement(10308..11498)
                     /gene="uidB"
                     /locus_tag="OO564_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6654015.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11495..13306)
                     /gene="uidA"
                     /locus_tag="OO564_002961"
     CDS             complement(11495..13306)
                     /gene="uidA"
                     /locus_tag="OO564_002961"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6654016.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
BASE COUNT         3438 a         3564 c         3250 g         3412 t
ORIGIN      
        1 gatacatccg tctgttccgg acattatctt ccaaacaagg tcttatccag ttggcggttt
       61 acattgctcc acagtcgtga attaatacga atagtattct gttgagggcg atgccaatga
      121 aaaaattgtt tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc
      181 tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt
      241 tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg agagatcccc ccacacgact
      301 ggggtaagac gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac
      361 tgagtaaata ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg
      421 ccctggacga tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag
      481 ttggtcctgt tccttatctt tattacctcc atcaaatgcg attgctggat atcgccctga
      541 tcgcttttat tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc
      601 aggatatgtt aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac
      661 gtatccactt tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg
      721 cggacaatat caacgcctta attgccagca aaaaacagct tattgacggt atcgctcacg
      781 aactgcgaac accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg
      841 ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc
      901 tgctgactta tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaaccagacc
      961 tgccgttgtg gctgtcaacg catctggcag atattcaggc agtaacgccc gataaaacgg
     1021 tacggataaa aacgctcatg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc
     1081 gcgtgctgga taatttgctc aataacgccc tgcgctactg ccattcaacg gttgaaacca
     1141 gcctgctact gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg
     1201 ccccagaaaa ccgcgaacat atctttgaac cttttgttcg cctcgatccc agccgggatc
     1261 gctcaaccgg cggctgcggg ctggggctgg caattgtcca ctctatagca ctggcaatgg
     1321 gcggtacggt taattgtgac atcagcgaac tgggtggtgc tcgcttctcg tttagctggc
     1381 cgttatggca taacatcccg caatttacct ctgcctgaca ctacgcgcac gatggtcaag
     1441 tcacaacgac tgtgctataa aataagtatg ttgtaactaa agtggttaat attatggcgc
     1501 gttacgatct cgtagaccga ctcaacacta cctttcgcca gatggaacaa gagctggcta
     1561 catttgccgc tcatcttgag caacacaagc tattggttgc ccgcgtgttc tctttgccgg
     1621 aggtaaaaaa agaggatgag cataatccgc ttaatcgtat tgaggtaaaa caacatctcg
     1681 gcaacgacgc gcagtcgctg gcgttgcgtc atttccgcca tttatttatt caacaacagt
     1741 ccgaaaatcg cagcagcaag gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg
     1801 ataacctttc gcaagcagcg ttggtcagtc atattcagca catcaataaa ctcaagacca
     1861 cgttcgagca tatcgtcacg gttgaatcag aactccccac cgcggcacgt tttgaatggg
     1921 tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta ccgcacgctc accgttctgc
     1981 acgaccccgc cactttacgc tttggttggg ctaataaaca tatcattaag aatttgcatc
     2041 gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc accacgtagt gtcgcaccgt
     2101 ggacgcgcga ggagtggcaa agaaaactgg agcgagagta tcaggatatc gctgccctgc
     2161 cacagaacgc gaagttaaaa atcaaacgtc cagtgaaggt gcagccgatt gcccgcgtct
     2221 ggtacaaagg agatcaaaaa caagtccaac acgcctgccc tacaccactg attgcactga
     2281 ttaatcggga taatggcgcg ggcgtgccgg acgttggtga gttgttaaat tacgatgccg
     2341 acaatgtgca gcaccgttat aaacctcaag cgcagccgct tcgtttgatc attccacggc
     2401 tgcacctgta tgttgcagat taacgcccgg cttttatact gccgaccatc tgttctggcc
     2461 gtacccagct gtcaaactcg gcttcgctaa gataccccag cgcaagggcc gcagctttta
     2521 aggtcagccc ttctttatgc gcttttttgg cgatctccgc ggctttgtca taaccaatgt
     2581 gggtgttaag cgcagtcacc agcatcagcg attcattgag taattgattg attcgctcac
     2641 gattcggttc aatacccact gcgcagtgtt tgttaaaact ttccatgcca tctgccagca
     2701 agcgcaccga ttgcaggaaa ttatggatca ccatcggacg gaagacgttc agttcaaagt
     2761 taccggaagc gccacccatg ttgatcgcca cgtcgttccc cattacctga cagcagagca
     2821 tggttaatgc ttcacactgg gttgggttca ctttccccgg catgattgag ctgcccggct
     2881 cattttccgg gattgagatt tcaccaattc cgcagcgcgg gccagaggcc agccagcgga
     2941 catcattggc gattttcatc agtgacgcag ccaacccttt caacgcgccg tgcgcctgaa
     3001 ccagggcatc acaggtcgca agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac
     3061 aggtaatgac tgccagttca tctgctacgc gccgcgcata ctccggatgg gtatttagtc
     3121 cagtacccac cgctgtaccg ccaagagcca gttccgctac gtgaggcagg ctgtattcga
     3181 tatgtttgag attatgctcg agcatcgcta cccagccgga aatctcctgc cctagtgtta
     3241 gcggcgtggc gtcctgcaag tgggttcgac cgattttgac gatatcggca aatgcacgcg
     3301 atttttcact cagcgtctgt gtcagggttt taagctgcgg aatgagttgc ttgcgcagtg
     3361 ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc gttggaactt tggcttttgt
     3421 tcacgtcgtc gttagggtga actttacgtt ccatcccgcg cacaccgccg agtaattcac
     3481 tggcccggtt agccagcact tcgttcatgt tcatgttact ttgcgtgccg gagccggtct
     3541 gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct
     3601 gccgaatggc gctcgctttc tcttcagaca acaagtctaa atcttcatta acttttgccg
     3661 ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa
     3721 tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca cagcttatct gccgggacat
     3781 caatcgcccc catcgaatct ttttcgctgc gtactgtatt catggcctgc tcctcacctg
     3841 attaattttt tctttctgtt ttgctttcgt taagcaactt ttagctcact cattatttac
     3901 tatttgataa caaatgtttg gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc
     3961 tctgtttatt tcacacagcg agtgcactgt gtgagttgta tctgctggaa gaagtcattt
     4021 cctttatcat ccacaaggat aaacgccggg aaatcttcca cttcaatttt ccagatggct
     4081 tccattccca gttccggata ttcaacacat tccaggctct taatacttcc ctgcgccaat
     4141 acagcggccg gaccaccgat actgccaagg tagaagccgc cgtgtttttt acaggcatcc
     4201 gtcacctgct ggctgcggtt gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc
     4261 agttgatcga cataagaatc catccgtccg gcggtcgttg ggccaagaga accggaggca
     4321 taaccttccg gcgttttggc cggacccgcg tagtaaatcg gatgatcttt aatgtattgc
     4381 ggcagccctt caccgttatc catccgctct ttcagtttgg cgtgagcaat atcacgacca
     4441 acgataatcg tgccgttaag cgataagcgt gtagaaacgg gatactgcga caactgtgcg
     4501 aggatctctt tcatcggacg gttaaggtca acgcgcaccg cttcgccttc tcccgcttta
     4561 cgcagctctt cagggatata tttacctgga ttatgttcca gtttttcgat ccagatcccc
     4621 tgacggttga tcttcgcttt gatattacgg tcagcagagc aggaaacacc cataccaacc
     4681 ggacaggatg cgccgtgacg tggcaggcga atcacgcgga tgtcgtgagc gaagtattta
     4741 ccaccaaact gcgcacccag accgagattt tgcgcttcga tcagtaattc tttttccagt
     4801 tccacatcgc ggaacgcctg accgtgctca ttcccttccg ttggcagttc atcatagtat
     4861 ttcgcggaag ccagtttcac cgttttaagg ttcgtttctg cagaagttcc accaataacg
     4921 aacgcaatat gatacggagg acaggccgcg gtacccagcg tgcgcatctt ctcaaccagg
     4981 taatttttca gtttccccgg cgtcagtaac gctttggttt cctgatagag atacgtcttg
     5041 tttgccgaac caccaccttt tgcgatacag aggaatttgt attcgtcgcc atcaacggca
     5101 taaagatcga tctgcgctgg cagattggtg ccggtattca cttctttata catatccagc
     5161 ggcgcgtttt gcgagtagcg cagattatct tcgatataag tgttatagac accgcgcgcc
     5221 agcgccgctt catcaccacc accggtccat acacgctgcc cttttttacc aacaataatc
     5281 gcggtgccgg tatcctgaca ggttggcaga acgcctttcg ccgcgatgtc ggagttacgc
     5341 aggaattgca gcgccacata tttatcattt tcgctggcct ccgggtcacg cagaatgtcg
     5401 gccacctgtt gttggtgagc cggacgcagc ataaacgacg catcatgaaa cgcctgacgc
     5461 gccaacagag ttaacgcttc gggtgcgact ttcaaaatct cctgcccttc aaattcagat
     5521 acgctaacgt gttcactggt tagcaggtaa tactcagtat catctttttt gagtggaaaa
     5581 ggagactgat aatgaaaggg tttgtttgac attgttctct cacttactgc ctggtttggt
     5641 tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga gtaacaatat cctacacagt
     5701 tttttaacaa aaactgagac tagtacgact ttttgcggct ccaggttact tcccgtagga
     5761 ttcttgcttt aatagcggga ttaatttcca cattaaaaca gggattgatc atgcaaaaac
     5821 tcattaactc agtgcaaaac tatgcctggg gtagcaaaac ggcgttgact gaactttatg
     5881 gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa
     5941 gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga
     6001 gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc
     6061 tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca
     6121 attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc
     6181 gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg
     6241 cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgccggtg
     6301 cacatccggc gattgctcac tttttacaac agccacatgc cgaacgttta agcgaactgt
     6361 tcgccaacct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat
     6421 cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt
     6481 acccggaaga cagcggcctg ttctccccgc tgttactgaa tgtggtgaaa ttgaatcctg
     6541 gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg
     6601 aagtgatggc gaactccgat aacgtgctgc gtgcaggtct gacgcctaaa tacattgata
     6661 ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc
     6721 agccggtgaa acaaggtgca gaactgggct tcccgattcc tgtggatgat tttgccttct
     6781 cgctgcatga ccttagggat aaagaaacca ccatcagcca gcagagtgcc gccattttgt
     6841 tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg
     6901 gtgaatcagc gtttattgcc gccaacaaat caccggtgac tgtcaaaggc cacggccgtt
     6961 tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag
     7021 cttattaaag gcttataaca ccttcaggcg gccagtccgc ctgatttcat tttatggata
     7081 atcattatga ataaatcgct ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg
     7141 acaggcggcg catggtatac aggcaagaag attgaaaccc atctcgaaga catggtcgcg
     7201 caggcgaacg cgcaactcaa actgacagct cctgaatcca acctggaagt gagttatcaa
     7261 aactatcatc gcggcgtatt cagcagccag ttgcaactgt tggtgaaacc cattgctggg
     7321 aaagaaaatc cgtggattaa aagcggtcag agcgtcatct tcaacgaatc ggttgatcat
     7381 ggtcccttcc cgcttgccca gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt
     7441 caaaccacgc tggttaataa cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa
     7501 acgccttttg agattaactc gcgcattggt tacagcggtg attccagttc cgatatttcg
     7561 ctcaatccac tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc
     7621 cagttaaatg ctgacagaga cggcaaagcc atctcccttt ccggggaggc gcaaagtggt
     7681 cggatagacg cagttaacga atacaaccag aaagtgcagt tgacctttaa taatctgaaa
     7741 accgacggtt ccagcacgct gggaagtttt ggtgagcgcg taggaaacca aaaactgtca
     7801 ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg cactgctgga aggcatggag
     7861 atcagcggta aatcggatct ggtcaatgac ggtaaaacga tcaatagcca actggattac
     7921 tcgctaaaca gcctgaaggt acagaatcag gatctgggca gcggcaagct gactttaaaa
     7981 gtcggccaga ttgatggtga agcctggcat cagtttagcc agcaatataa cgcgcaaact
     8041 caggcgctgc tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg
     8101 acggaagcct tctttagcgc cctgccgctg atgttgaaag gcgatccggt gattactatc
     8161 gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg
     8221 aaagatccgg caacgactaa agaagcgccg caaacgctgg cgcaggaagt agatcgttcg
     8281 gttaaatctc tggatgcgaa actgaccatt ccggtggata tggcaactga gttgatgact
     8341 caggtagcga agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa
     8401 gttgaaggtg catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc
     8461 atcaccacca gcctgcaata tgctaacggt cagataacgt taaatgggca gaaaatgtcg
     8521 ttagaagatt ttgttggcat gtttgcgatg ccagctctta acgttccggc tgtaccggca
     8581 attccgcagc agtaattcac ggccaaaacg ccggagaatt ttcactccgg cgttttcatc
     8641 tgaaaacaaa ccagagattg cctgatgcgc tacgcttatc aggcctacct tttcctgcaa
     8701 cactttgaat ttatgagttt ttgtaggctg gataaggcgt ttacgccgca tccggcataa
     8761 aaaacgcgca ctttgtcaac aatctgaaac gccggaggtt tttctctccg gcgttatttc
     8821 ttacttcagc ataaagtcat actgaatact cattttcaca tcccagtaat tcaggcgatc
     8881 gcccagcgta ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa agaaattcat
     8941 cccttgtaac ggcccttcgc tgacccgata gttcatacca cctaagatac ccgcgccttc
     9001 aatccgcaca tctcgtttac tggattcata gccgcgagtc acaatcggtg caaatgtcag
     9061 cgtaaattgc ggagttacac ggtagttgac acctaattgc caggactgca ttccttcacg
     9121 gttacgatca agacttaatg aaaagggata gccaatgtcg gtatccacca cggcaccatt
     9181 cattgcatgg cgcaaataac caccactgcc aaatacgctt ccccacggcg cattccaggt
     9241 caattgtcca ctaaccaacg ccgtttcatt gggctggctg gtattacgac ttaaaccttc
     9301 gagctgcgcg taaaagccga gcaactcccc tttcaacgta taattcgggt cgagcggtat
     9361 gcttttctcg agaatacttt gtatctgatt ggaaacatct ttctgtaccg atgcccccag
     9421 agaatagcga ctggtatcat catgccaact ggcggctaaa aaagaacgtg cgcgatgttt
     9481 atcgtatttt cccgtctggc tgttatacac cccttcatca cgaataccgg ttgttgaatc
     9541 agtccataaa aatactcgtg aacgccacat tccccacaga tcaatatttg gtgttaaagc
     9601 ccaataacct aatgctcctt cagaaactgt cggtaatgta cgttgcgaac tgccagccat
     9661 taacggaata cgataaggta agctaccata actataatcc gtcccgaagc gacctaattt
     9721 aagcaggcta ttttcactgg gttttatttt tactgccccc agggcaaagc caaaactttt
     9781 atcaccatca agataccacc gggtactcat atcagcacga gcacctaatt tataaacata
     9841 atacgctccg ccttcaacgc caataatatt ggaataataa ccactattga aatcgagcaa
     9901 tcctccctgt acccatgcgt aaatatctct tcccgcgcca gcactgggct tatcagccct
     9961 gcgcaattcg tttttcagtc tgatgcgtag tcccgcttca tcgtcagcca gttgcgccac
    10021 gtaagcagag gtaaggccag aggcagccgt caggcaaata acggccatgg ccactatttt
    10081 tctcatagtt gcacctttga tgttctgatt atttttattg aatattaatt agtgatatcg
    10141 ctgattaatt gctgctgcac ttttttacga ttatcaattt caaccacgat ttctttgaat
    10201 tttttatccg tgagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa
    10261 ggtactaagg caattgatgt gcggatgccc ataataactt ccggcgttta cacctgattg
    10321 gcgatatatc cgcttaatcc caaaataaag gcaggaattg aacctccgat tgcctgaccg
    10381 catttacggg taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg
    10441 tattcaccgt attctacggt atcagcttcc agtgcccaca tcacagtcat agtaacgccc
    10501 tgaccaattg aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc
    10561 cagaagaata gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt
    10621 ttaccgatcc tcgcgaccat ccccggcaca agcggtgccg atgccacagt accaaccagg
    10681 ttttgcacca gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag
    10741 aacaacgacg aggcgctgac cgcgaaggtc gaaatcagca cacacagcgc accgatgcac
    10801 aacataaaca gcgggcgatt ccgtttcagg gtttgcaaac tgatcttcaa tgacggctgc
    10861 gccacgatac gtaccacatt ttcacgcgtc gatttgaagc agatgaagta aagcaccatt
    10921 ccggcaatcg ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg
    10981 gagttcttaa tgctcggtcc tatcagaaat gccaggcata caaaggtcaa tgaagcggca
    11041 atcccacgag ccgcgcccag acgggcgcga gattgtggtt gttgggtcat cgcggtagca
    11101 agtgaaccat aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa
    11161 tatgcataca ccactttgct accatggctc cagtcggtcg gtacccagaa taccagcacg
    11221 ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg
    11281 gtattcacac tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc aaatacccgc
    11341 accagtaaca gcatggtgcc tgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa
    11401 ctcaatagga agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg
    11461 tagccgacga tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt
    11521 tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt
    11581 tgcggtcgcg agtgaagatc cctttcttgt taccgccaac gcgcaatatg ccttgcgagg
    11641 tcgcaaaatc ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga
    11701 cgcggtgata catatccagc catgcacact gatactcttc actccacatg tcggtgtaca
    11761 ttgagtgcag gccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca
    11821 gtttctcctg ccaggccagc agttcttttt ccagtacctt ctctgccgtt tccaaatcgc
    11881 cgctttggac ataccaaccg taataacggt tcaggcacag cacatcaaag agatcgctga
    11941 tggtatcggt gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt
    12001 cgagtttacg cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg
    12061 tatccggttc gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta
    12121 tcagctcttt aatcgcctgt aagtgcgcct gctgagtttc cccgttgaca gcctcttcgc
    12181 tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc
    12241 cgacagctgc agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct
    12301 cttcagcgta agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg
    12361 cgtgatcgtg caccatcagc acgttatcga atcctttgcc tcgcaaatcc gcatcttcat
    12421 gacgaccaaa gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca
    12481 ctgccactga ccggatgccg acgcgcagca ggtagatatc acactctgtc tggcttttgg
    12541 ctgtgacgca cagttcatag agataacctt cacccggttg ccagaggtgc ggattcacca
    12601 cttgcaaagt cccgctggtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca
    12661 gttcaacgct gatatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt
    12721 cttgcgcgac atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga
    12781 gcattacgct gcgatggatc ccggcatagt taaagaaacc atggaagtaa gactgctttt
    12841 tcttgccgtt ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt
    12901 tgttcacaca aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg
    12961 cttcaaatgg cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt
    13021 tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg
    13081 gtataaagac ttcgcgctga taccagacgt tgcccacata attacgaata tctgcatcgg
    13141 cgaactgatc gttaaaactg cctggcactg caattgcccg gctttcttgt aacgcgcttt
    13201 cccaccaacg ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt
    13261 cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat
    13321 taagataaaa atactggtca acctttaatc tgattagtta accaattctg tgttgcacac
    13381 agttatctgt gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc
    13441 aaaaaaccaa aaactgcaat acccacagcg tgatatggat cgcattaatc tttaaaacag
    13501 cgttgacaga agccataaaa caagcaatcg agcgttcgtt cgagagtatt gggaacgata
    13561 aactctcatg acatatggct acggtgaata ttttgggggg gaattctccg aacacgggga
    13621 ggataatttt tgcacagaga aattacgata tgaaacgcgg gcac
//
LOCUS       DAJTYI010000105        13625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYI010000105 DAJTYI010000000
VERSION     DAJTYI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..429)
                     /locus_tag="OO564_002962"
     CDS             complement(<1..429)
                     /locus_tag="OO564_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6654017.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRK"
     gene            739..2316
                     /locus_tag="OO564_002963"
     CDS             739..2316
                     /locus_tag="OO564_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HCR6654018.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            complement(2506..2646)
                     /locus_tag="OO564_002964"
                     /pseudo
     CDS             complement(2506..2646)
                     /locus_tag="OO564_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-kinase"
     gene            complement(2646..3449)
                     /locus_tag="OO564_002965"
                     /note="aph(3'')-Ib"
     CDS             complement(2646..3449)
                     /locus_tag="OO564_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6654019.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3510..4325)
                     /gene="sul2"
                     /locus_tag="OO564_002966"
     CDS             complement(3510..4325)
                     /gene="sul2"
                     /locus_tag="OO564_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6654020.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5514..5663
                     /locus_tag="OO564_002967"
     CDS             5514..5663
                     /locus_tag="OO564_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654021.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(6408..6848)
                     /locus_tag="OO564_002968"
     CDS             complement(6408..6848)
                     /locus_tag="OO564_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6654022.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETC
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            7066..7455
                     /locus_tag="OO564_002969"
     CDS             7066..7455
                     /locus_tag="OO564_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654023.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            7499..7903
                     /locus_tag="OO564_002970"
                     /pseudo
     CDS             7499..7903
                     /locus_tag="OO564_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(8010..8714)
                     /locus_tag="OO564_002971"
                     /pseudo
     CDS             complement(8010..8714)
                     /locus_tag="OO564_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
     gene            complement(8744..10495)
                     /locus_tag="OO564_002972"
                     /pseudo
     CDS             complement(8744..10495)
                     /locus_tag="OO564_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            10484..10891
                     /locus_tag="OO564_002973"
     CDS             10484..10891
                     /locus_tag="OO564_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR6654024.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            11029..11913
                     /locus_tag="OO564_002974"
     CDS             11029..11913
                     /locus_tag="OO564_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR6654025.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(11945..13144)
                     /gene="tet(A)"
                     /locus_tag="OO564_002975"
     CDS             complement(11945..13144)
                     /gene="tet(A)"
                     /locus_tag="OO564_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6654026.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            13250..>13625
                     /gene="tetR(A)"
                     /locus_tag="OO564_002976"
     CDS             13250..>13625
                     /gene="tetR(A)"
                     /locus_tag="OO564_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6654027.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGF"
BASE COUNT         2979 a         3753 c         3859 g         3034 t
ORIGIN      
        1 cttgcggccc cggtacgcgc cacgctgctt ggccaaggcg atgccctcac gctgccgctc
       61 gcggatcagg gcgcgctcga actcagcgaa ggcccccatc accgacagca tcaggttggc
      121 catcggcgag tcctcgccag tgaacaccag gccctccttc aggaactcga tgcgcacgcc
      181 gcgctgagtc agcttctgta ccaagcgacg caggtcatcg aggttgcggg ccagccgatc
      241 catgctgtgc accaccactg tatcgccttc gcggacgaag ctcagcagcg cttcgagctg
      301 ggggcgctgg gtgtccttgc ccgatgcctt gtcggtgaac accttgctca cctgggtttg
      361 ttccagctgg cgttccgggt tctggtcaaa gctgctgacc cggacgtagc cgatgcggtg
      421 cccctgcacg atgtctcctt ggttgaaggc ggcttaagtg cactttctgt tccgttgtgc
      481 ctcaaagccc atttctgtca ggctgaaatc tataaccttc gcgggcatgt gtcaaaaaat
      541 gggaaagcag actctattct gacgaagcgg cgcggccctg cctgacatca agttagggta
      601 tagcctagat tgacatgcgc gatgcaaccc ttaacttgct tgcacctatc gtttccatgc
      661 tagctttatc gtaacgctca agaaattggg cttaatgccg aaaacaataa aataaaacag
      721 ccaacccttg aggttcttat gcgccagaat ttaccagtga cgggtcgaaa cttagaactc
      781 ccaaaagatg ccaatattct ttcgactacc tcccctcaaa gccatatcac gtacgttaat
      841 cctgacttca ttaaaatcag tggtttcact gaggaagaac tattaggcca gcctcacaac
      901 atcgtaagac acccagatat gccgcctgct gcatttgagc atatgtggag tacattaaaa
      961 tctggccgct catggatggg gctagtaaaa aatcgctgta aaaatggcga ccactattgg
     1021 gtaagtgctt atgtaacgcc aatagctaag aatggttcga ttgttgaata ccagtctgta
     1081 aggaccaagc ctgaacctga gcaggttttg gctgcggaaa aattatatgc tcaattgaga
     1141 agcgggaagg ccgcgaggcc gaaattggct gctagctttt ccgtgaaaat actcttgctc
     1201 atatggggta gtattatatc aagcgcaatg gctgccggca tgcttactga tacatcaata
     1261 agcagcttat tgttagccac tttaatgtca ggaagcttaa gctctgttag tgttttggct
     1321 attctctctc ctcttggaag actggttgaa agagccagga atatttccaa taacccatta
     1381 agtcaatccc tctacactgg gcgcaccgat gagtttggcc aaatagagtt tgctttacga
     1441 atgatgcaag ctgaaacagg cgccatagta ggtcgcatag gtgatgcatc aaatcggctt
     1501 agcgaacaca cccgaggcct actaaaggat attgagtcaa gcaatgtact tacagttgag
     1561 cagcaggcag agacagatca aatagcaacg gcagtaaacc aaatggtggc aagcattcaa
     1621 gaggttgcga gcaatgcaca gcatgctgca gatgcggccg gaagagcaga tactgagacg
     1681 gcatctggcc agcgtctggt agcccacaca agccagtcaa tcactgccct tgaaggtgaa
     1741 attaggcaag ccactcaggt tattcatgag cttgaaggtc aaagtaacga gatatcaaaa
     1801 gttcttgacg ttatacgagg gatcgccgag caaacgaatt tgttggcact caacgcagca
     1861 attgaggccg cgcgtgctgg tgagcagggg cgtggttttg ctgttgtcgc cgatgaggtt
     1921 cgcagtcttg ctgctcgcac acagcaatcg acaacggata ttcaaagcat gatcagcgct
     1981 ctacaagagc gagcgcaatc cgctgttaca gtcatggagc aaagtagtcg gcaagcgcac
     2041 acgagtgtag ctcacgcaga ggaagcagct acagctcttg atggaattgg ccaacgcgtt
     2101 aacgaaatta ccgacatgaa cgcgcaaata gcgactgcgg tcgagcagca gggagcagta
     2161 agtgaagaca taaaccgcag tattatcaat atacgcgatg ctgctgatac caatgtacag
     2221 accgggcaga ataatttgca aagtgcgaaa tctgtcgctc agttaactag cgctctgagc
     2281 gaactggcaa aacagttttg ggaaaaacga ggataacgct tttcagtatc tcgacaggga
     2341 taacttactt taccatcggt tatccctttt agccgcacaa attttagcca tggcttttca
     2401 ggaagtcagg gctatcagaa tggccttaga aagcctagtc aaagagctgt cacgagaaca
     2461 ccgttagctt agcgtacgat tttttccgaa ttctgcggtt cccccttcta taggtttcaa
     2521 tcccttgacg attgcaggag tcccgtctgg caatgaaact ttccaaacga ggctggaaaa
     2581 ggtgtccgca atgagaacag gttgcgaaac gtgccaatga gcaggaaaaa caggcggcat
     2641 gaacatcaac cccaagtcag agggtccaat cgcagataga aggcaaggcg ttcgcggtcg
     2701 ggggcttcga tccccaatac attgaatagg acagcgaagg cgcgctctgc ttcatctggc
     2761 gctgcccagt tctcttcggc gttagcaatc atgagtgcca aatcggcata gcgatctgct
     2821 gttccgagcc gcccaaggtc gatcagaccc gtgcattgaa gagttttagg gtccaccatg
     2881 aagttcggca tgcagggatc accatggcaa acaaccatat cggtgcgctc ttggtcgagc
     2941 cgcaccggta gctctcgttc gacacgagcc aaaagatcga gctgcggcgt actcttgtcc
     3001 tcgtccggta agaagtcggg attgacggca ttgcgggaca ccacatcaac ggcgcgtccg
     3061 aacattcgcg acagcctgcg ctcaaacgga cattgatcaa ccgataggct gtgaacagcg
     3121 ccaagttgct gccccattga cggccacgct ttgagcaaat ccgctccaga cagatcagcc
     3181 gccggtactc ccggaattgc cgttatcacc aagcatgcac cctcctgttc ctcctgccag
     3241 ttgatgacct cggggcaagc cacacctcga cctttgagcc aaatgaggcg gtcacgctct
     3301 ccagcgagct caccgcggcg ggaagcaggt gcgattttcg cgaaggcatg cccgtcacca
     3361 cgtcgaaaaa caaaatcacc agattctccg cctctgacag gcaaccagtc agaatgcgat
     3421 tcaccaaaaa aaatattagt tcgattcaat ggaggttcct tcagttttct gatgaagcgc
     3481 gaatatagag aaatatcccg aatgtgcagt taacgaattc ttgcggtttc tttcagcgcc
     3541 gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt gtgtgcggat gaagtcagct
     3601 ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg ccccgacatc ccccggacca
     3661 cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag acagaagcac cggcaaatcg
     3721 aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga gcgaggtttc gggagcagcc
     3781 cccagaaaaa accccatgcc gggatcaagg acaaggcggt tgcgtttgat accggcaccc
     3841 gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt gatccatgat gtcgccagcg
     3901 ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat gcataacgac gagtttggca
     3961 gatgatttcg ccaattgcgg atagaacgca gcgtctggaa aaccgcgaat atcattgaga
     4021 taggccacac cacgcgacaa ggcataggct tgcgtcgcgg gttgataact gtcgagcgag
     4081 acgggaatgc catctgcctt gagcgcgtcc agcaccggcg cgatacgcgc gatttctgtg
     4141 tcggacgaaa caggcgcggc gtcgggattg ctggatgccg gaccgaggtc gatcacatct
     4201 gccccctcgg ccatcagctt acgcgcctgc gcaatggctg cgtctggcgc cagataccgg
     4261 cctccatcgg agaaactgtc cgaggttatg ttgacgatgc cgaaaatgat gagcgattta
     4321 ttcatggggg cttctataat aataataatc gagcatgagt ctcatacgga tgctcgggtc
     4381 gaaagggaat ccccaggcga gtaacctgtt tgcggtgatc cgtttcatga tgatgtatct
     4441 gtagcatttt ggaaataaat atatcatctc tcaataagta gatatattta tcttttttgc
     4501 ttctttggtg ctgcaagtca ccaatacgag ggaaacctgt tacggagcgg tttaaccgta
     4561 atcaacagtg actggtgtca tgttcggaag attaattgat agtaatctac aaaggatctt
     4621 cttgagatcc ttttcaatga atcgtaatct cttgctctgt aaacgaaaaa accgcctggg
     4681 gaggcggttt gttcgaaggt taagtcagtt ggggaactgc ttaacccggt aacagggttc
     4741 acgagaccgc cattaccaaa actgttcttt cagtgtagcc gcagtcaggc ctctacttca
     4801 agacctctta atctatcttc tcgtgcactc ctgttactaa tggctgctgc cagtggcgtt
     4861 ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac cggacaaggc gcagcggtcg
     4921 gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgcctt cacggaactg
     4981 agtgtcaggc gtggaatgag aaaccgcggc cataacagcg gaatgacacc ggtaaaccga
     5041 aaggcaggaa caggagagcg cacgagggag ctaccagggg gaaacgcctg ggatctttat
     5101 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttttgcgatg cttgtcaggg
     5161 gggcggagcc tatggaaaaa cggcttcgcc gtggccttat ctcttctttg ctaagtgtct
     5221 tcctggtatc ttccaggaaa tttccgtccc cgtccgtaag ccggtaccgc tcgccgcagc
     5281 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatctcct gtatcgcata
     5341 ttctgctgac gcgccggtgc tgcctttttt ctcctgccac atgaagcact tcactgattt
     5401 tcgcaatcgt ggcaacatag taagccagta tacactccgc tagcgctaca tgactggttc
     5461 agggctgcgc cccgaaaccc gctaacaacc tctcagctac tggatatcac cccatgccat
     5521 ctgaatatga tgaacaactg gattcgactg atacgcagct tcccggcgaa tatgaccttt
     5581 ttgatttaat ggcgcaggtt actgaagaga acctgcatgc ccctgtggat tttgggtcgc
     5641 ctgtcggccg ggaagcgtta tagctttttc gcttgcccgc gtacgtggta atactccggt
     5701 attacctttt atttctttat tttcatgtaa ttacagtgga tttttaatac ccgggtatta
     5761 ctcgtgaaaa cgaaatattc cttttttttc ttttaattca tgcagttgcg atgaaaatcg
     5821 cggctgcgtt aggtgtatga ccattttgag gttgaaaaat aatcaaccat ggcgggtgat
     5881 ttacgcaggt taaggggaga tcgtgacaaa aaaagcgttc ctcactgtat cgttcatgcc
     5941 tgcatgaacg ggttattctt cctgactaca gaattacatc gctccttcag ttcatacatc
     6001 acttttacat cacttataca tcacttttac atcattcctt cagctcatac gtcactttca
     6061 tcctctgtgc catttgtaca tcaccccctt cctgaacgtg ctgacagcgg gttttccggc
     6121 tgcgccggca acgcttcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat
     6181 gggaagaacg ccggaaacag gacttacccc ggctgaatgg tgtgaatttc cgcgctatgc
     6241 acttgtgcgc atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt
     6301 cgggtggttt gttgcctgtt ttaccggttt cccgtcagaa acgccctgag ggcctctcag
     6361 gcggtgctga taacagatgt tatggtaaat cagagtcacc tgcactgtta ctctgaaccg
     6421 gagcgaaaca gccgcgacca gaatccccgc tttttttcct gtgccagcgc gtcagccaga
     6481 cggctaattt cggcctgtag ttgttccctc tcctcatttc gcaggcggtt ttctgcttcc
     6541 cgcgccatct tgtcttcaag cattagggta acagtctgct gcagagcgtc cagtttctgc
     6601 tgaatgacgt ctagctgagc attttccgcc tggctaacga cagatttgga gtgtccctcc
     6661 ttgtgccttt ttggtgtctc agaagtgcgc agctcgccat aggcccgaat cagttcacag
     6721 gtctcgaact cccggcgatc attttttcct gtgcgatagc tgacaaggcc tttagccatg
     6781 tcacgatata attgcgagcg cgactttcct gttaatttca gtgtctcttg aactgtgtgc
     6841 catgccataa aagtgacagt gtctcctgtg tgtaccgtat tgtaacagca gaacatatta
     6901 ctgttttaac acaaaaggtc tgaggctgcc cgtgaggcgt gaaaaataca caaaaaacct
     6961 gttgacggtg tggttttgac gggcgtagta tttcgaatac tgtatataaa cacattgtta
     7021 taaatacagt ttttgctgtt tttaaaagtc aaagaggata attttatgag tacagagtta
     7081 aatagcggta ctgtcggtgg ttttggtcac atgggaccaa atgaccccta caatgatggt
     7141 catagcggct ctggaagttc gggaggtagt tttaacggtg gtggtaatgg tggccatgat
     7201 aatagtagtg gttcatctta tactgcatcc tttggtctca tcactggccg acacccgata
     7261 actgtcccag gtacgaagaa tattaattct gtcgatcttt ctgctgtcat taataacatt
     7321 atacatcatg aaggtgcacg caataacatg tacctagata ctgcgaacaa tgtaagagtg
     7381 ggagtgacgg gcactggctg gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa
     7441 agaactttcc gctaagcgat agactgtatg taaacacagt attgcaagga cgcggaacat
     7501 gcctcatgtg gcggccagga cggccagccg ggatcgggat actggtcgtt accagagcca
     7561 ccgacccgag caaacccttc tctatcagat cgttgacgag tattacccgg cattcgctgc
     7621 gcttatggca gagcagggaa aggaattgcc gggctatgtg caacgggaat ttgaagaatt
     7681 tctccaatgc gggcggctgg agcatggctt tctacgggtt cgctgcgagt cttgccacgc
     7741 cgagcacctg gtcgctttca gctgtaagcg tcgcggtttc tgcccgagct gtggggcgcg
     7801 gcggatggcc gaaagtgccg ccttgctggt tgatgaagta ctgcctgaac aacccatgcg
     7861 tcagtgggtg ttgagcttcc cgtttcagct gcgtttcctg tttggggtcg tttgcgggaa
     7921 ggggcggaat cctacgctaa ggctttggcc agcgatattc tccggtgaga ttgatgtgtt
     7981 cccaggggat aggagaagtc gcttgatatc tagtatgacg tctgtcgcac ctgcttgatc
     8041 gcggccgcga tagctagatc gcgttgctcc tcttctccat ccgcgttcca agctgcggaa
     8101 aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg gatcgacgtc cagcgcacga
     8161 gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga gacaaaggtc gtctctgtca
     8221 gccggatcgt agaacatatt ggcggcgcca aagcccactt caccgaccag accgacggga
     8281 tctatcacca gccagccgcg actggagaac atgatgtttt catgatgcag atcgccatgt
     8341 agcccacgca gttccgaggc attgctcatc atttgatcgg ctataatcgc cgcgtggacg
     8401 tagtcagttt gacaacctgc gttttgatca tcgcgcgccc gctgaaacaa agctgcaaag
     8461 cgatcccgga tcgggagaag ggcagaaggc aggggttcct cagatgcggc atacagcttc
     8521 gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc cgtgctcggc aacgatgtga
     8581 gagagcattc gctccccggc atattcgagc aacatcagat tgttctcacg accgagcaac
     8641 cggactgctc ccctcccatt gcgccatacc agatagtcgg ccccgcgcag ttcatcagca
     8701 atgtcttcta ggggagcccg cagaattcgg aaaaaatcgt acgctaaggt tttccgggca
     8761 tccgtaaggg ccgaaacttc ccgtcttcca gtctgcggct ctgccgccag acgtaatcgc
     8821 cggttaggtt gatgtgctcc cagcccagcg gcgacaggaa ttgcagcagc tcgccgtcca
     8881 ccggcttgcc ggcctcgacc aacccctggg tggcgcgttc caggtacacc gtgttccaca
     8941 gcacgatagc cgccgtcacc aggttgaggc cgctggcccg gtagcgctgc tgctcgaagc
     9001 tccgatccct gatttcccca aggcggttga agaacaccgc cctggccagc gagttgcgcg
     9061 cctcaccttt gttcaggccg gcatgcacgc ggcggcgcag ttcaacactt tgcagccagt
     9121 ccaggatgaa cagcgtgcgc tcgatccggc ccagctcgcg cagggccacg gccagtccgt
     9181 tctggcgcgg gtagctgccg agcttgcgca gcatcagcga ggcggtgacg gtgccctgct
     9241 tgatcgagct ggccaggcgc aggatgtcgt cccagtgggc acgcacgtgc ttgatgttca
     9301 gggtgccgcc gatcagcggg cgcaacgtcg ggtaggcttg cacgccctgc ggcacgtaca
     9361 gcttggtttc gccgaggtcg cggatgcgcg gcgcgaagcg gaagcctagc aggtgcatca
     9421 gggcaaagac gtgatcggtg aagccggccg tgtcggtgta gtgctcctcg atccgcaggt
     9481 cggactcgtg gtacagcagg ccgtcgagca cataggtgga atcgcggacg ccgacattca
     9541 ccacgcgggt gctgaacggc gcgtactggt cggagatatg ggtatagaac agccgtcccg
     9601 gctcgctacc gtacttcggg ttgacgtgcc cggtgctctc gccccggcca cccgcgcgga
     9661 agcgctggcc atcggaggat gaggtcgtgc cgtcgcccca gtgggcggca aaggcgtggc
     9721 gatactggtg gttgaccagc tcggccaagg ccgccgaata ggtttcgtcg cggatgtgcc
     9781 aggcttgcag ccaggacagc ttggcgtagg tcaggccggg gctcgactcg gccatcttgg
     9841 tcagcccgag gttgatcgca tcaccgagga ttgcggacag cagcaacgtc ctgtctttgg
     9901 cctcggcccc gtccttcaag tgggtgaagt ggcggctgaa gcccgtccag tcgtccacgt
     9961 ccatcagcag ttcggtgatc ttgatgcgcg gcagtaactg gctggtttgg tcgatcagcg
    10021 cctgcgcccg atccggcacc gccgcatcca gcggggtgat tttcagccct gactcggtga
    10081 ggatggcatc gggcagctcg ttgtccttgg ccaggcgggt gacggtggcc aactgctcgt
    10141 ccagcagctg caaacgctct tccaggtact ggtcgctgtt cgggttgatc gccaggggca
    10201 gggcctgctc gcgcttgagt gcggcgaact tctcggccgg cagcaggtag tcgtcgaagt
    10261 cgcggaactg ccgcgagccc ttgacccaga tgtcgccgga gcgcagggcg ttcttcagct
    10321 cggacagggc gcagatttcg tagaatttcc ggtcgaggcc ttccggggtg atcaccagcg
    10381 gcttccagcg cggcttgatg aaggccgtgg gtgcatcggc cggcaccttg cgcaggttgt
    10441 cggcgttcat ctcacgcagg gtctgcacgg ctgccagcac gccttgcgcg gccggcccgt
    10501 gctgttcccc aacttttcgc tcggcgaaga gtatgaacat gcgccgccgg cgacgaatcg
    10561 ccaaatctca ccgtatctcc cgagcggacg atttcgcacc ggtctgcccg tcgaagggct
    10621 tgcgatcgaa cggggcgacc ttttctatgc atgtccgcga gccagcgtct tttatggcac
    10681 ggcgctcgac gccgaccttc ggacgcgcgg cgtaagcacg cttgtcatgg ccgggataag
    10741 caccaccggc gttgttcttt caagcgtcgc ctgggctagt gatgcggact acgacgtgcg
    10801 tttggtccag gactgctgct acgacccgga tcgggatgcc cacgaagctc tgttgcgttc
    10861 cgggttcggc ggacgtgtac aggtcgtgta attttcgggc ctcgcatgat cgcggccata
    10921 gccgcaggtg gattggtgcg aggcagggcc agtccaagtc agggtgcggt catcgcggcg
    10981 cctgcaactc tcaaaaaccc atcgccgtag cggaggtctt gtaatcgcat gtcattacgc
    11041 acgcccgatt tattgttcac agcgatagca cctgccattt ggggcagcac ctacattgtc
    11101 accacccaat acctgccgaa cttctcaccg atgacggtcg cgatgctgcg ggcgttgccg
    11161 gcgggtttat tgctcgtgat gatcgtccga cagattccaa cgggaatctg gtggatgcgc
    11221 atcttcatcc tcggcgcact taatatttcg ctattctgga gcttgttgtt tatttcggtc
    11281 taccgcctgc cgggcggggt cgcggcgacg gtaggcgctg tgcagccgct gatggtcgtg
    11341 ttcatctctg ccgctctgct aggtagcccg atacgattga tggcggtcct gggggctatt
    11401 tgcggaactg cgggcgtggc gctgttggtg ttgacaccaa acgcagcgct agatcctgtc
    11461 ggcgtcgcag cgggcctggc gggggcggtt tccatggcgt tcggaaccgt gctgacccgc
    11521 aagtggcaac ctcccgtgcc tctgctcacc tttaccgcct ggcaactggc ggccggagga
    11581 cttctgctcg ttccagtagc tttagtgttt gatccgccaa tcccgatgcc tacaggaacc
    11641 aatgttctcg gcctggcgtg gctcggcctg atcggagcgg gtttaaccta cttcctttgg
    11701 ttccggggga tctcgcgact cgaacctaca gttgtttcct tactgggctt tctcagcccg
    11761 gggaccgccg tgttgctagg atggttgttc ttggatcaga cgctgagtgc gcttcaaatc
    11821 atcggcgtcc tgctcgtgat cgggagtatc tggctgggcc aacgttccaa ccgcactcct
    11881 agggcgcgta tagcttgccg gaagtcgcct tgacccgcat ggcataggcc tatcgtttcc
    11941 acgatcagcg atcggctcgt tgccctgcgc cgctccaaag cccgcgacgc agcgccggca
    12001 ggcagagcaa gtagagggca gcgcctgcaa tccatgccca cccgttccac gttgttatag
    12061 aagccgcata gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg ctggtgagcg
    12121 ccgccagtga gccttgcagc tgcccctgac gttcctcatc cacctgcctg gacaacattg
    12181 cttgcagcgc cggcattccg atgccacccg aagcaagcag gaccatgatc gggaacgcca
    12241 tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca atcattccga
    12301 gcatgagtgc ccgcctttcg ccgagccggg cggctacagg gccggtgatc attgcctggg
    12361 cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc gcgtcccagt
    12421 gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg acaagttgca
    12481 tgatgaagaa gaccgccatc agggcggcga cgacggtcat gccccgggcc caccggaacg
    12541 aagcgagcgg gttgagagcc tcccggcgta acggccggcg ttcgcctttg tgcgactccg
    12601 gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa ggctgccgcg gcgaagaacg
    12661 gagcgtgggg ggagaaaccg cccatcagcc caccgagcac aggtcccgcg accatcccga
    12721 acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca tcagtgatat
    12781 cggcaatata agcgccggct accgccccag tcgccccggt gatgccggcc acgatccgcc
    12841 cgatatagag aacccaaagg aaaggcgccg tcgccatgat ggcgtagtcg acagcagcgc
    12901 cggccagcga gacgagcaag accggccgcc gcccgaaacg atccgacagc gcgcccagca
    12961 caggtgcgca ggcaaattgc atcaacgcat acagcgccag cagaatgcca tagtgggcgg
    13021 tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc ataatcaggc
    13081 cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt ctgttgggtt
    13141 tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga ttctttatca
    13201 ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca tgacaaagtt
    13261 gcagccgaat acagtgatcc gtgccgccct ggacctgttg aacgaggtcg gcgtagacgg
    13321 tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc tttactggca
    13381 cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg cggagaatca
    13441 tacgcattcg gtgccgagag ccgacgacga ctggcgctca tttctgatcg ggaatgcccg
    13501 cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg ccggcacgcg
    13561 accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct gcgaggcggg
    13621 ttttt
//
LOCUS       DAJTYI010000106        13611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYI010000106 DAJTYI010000000
VERSION     DAJTYI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1279
                     /gene="celF"
                     /locus_tag="OO564_002977"
                     /gene_synonym="chbF"
     CDS             <1..1279
                     /gene="celF"
                     /locus_tag="OO564_002977"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6654028.1"
                     /translation="KRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKLY
                     KTLDCREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIP
                     VIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIHDV
                     LMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRFAELLDGVASGQLKASGVKNIFDLPF
                     SEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPE
                     LKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTC
                     KLGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHS
                     DRDAELLAREMILAHEKWLPNFADCIAELKKAH"
     gene            1292..2050
                     /gene="chbG"
                     /locus_tag="OO564_002978"
     CDS             1292..2050
                     /gene="chbG"
                     /locus_tag="OO564_002978"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6654029.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2097..4358)
                     /gene="katE"
                     /locus_tag="OO564_002979"
     CDS             complement(2097..4358)
                     /gene="katE"
                     /locus_tag="OO564_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6654030.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKDVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4541..4804
                     /gene="cedA"
                     /locus_tag="OO564_002980"
     CDS             4541..4804
                     /gene="cedA"
                     /locus_tag="OO564_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6654031.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5081..5897
                     /locus_tag="OO564_002981"
                     /pseudo
     CDS             5081..5897
                     /locus_tag="OO564_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5901..7291)
                     /gene="tcyP"
                     /locus_tag="OO564_002982"
                     /pseudo
     CDS             complement(5901..7291)
                     /gene="tcyP"
                     /locus_tag="OO564_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7424..8014)
                     /locus_tag="OO564_002983"
     CDS             complement(7424..8014)
                     /locus_tag="OO564_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6654032.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8177..8844)
                     /gene="hxpB"
                     /locus_tag="OO564_002984"
                     /pseudo
     CDS             complement(8177..8844)
                     /gene="hxpB"
                     /locus_tag="OO564_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            8991..9527
                     /locus_tag="OO564_002985"
     CDS             8991..9527
                     /locus_tag="OO564_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6654033.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9568..10428)
                     /locus_tag="OO564_002986"
     CDS             complement(9568..10428)
                     /locus_tag="OO564_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6654034.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10534..10824)
                     /gene="ghoS"
                     /locus_tag="OO564_002987"
     CDS             complement(10534..10824)
                     /gene="ghoS"
                     /locus_tag="OO564_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6654035.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(10925..11854)
                     /gene="pfkB"
                     /locus_tag="OO564_002988"
     CDS             complement(10925..11854)
                     /gene="pfkB"
                     /locus_tag="OO564_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6654036.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12140..12898
                     /locus_tag="OO564_002989"
     CDS             12140..12898
                     /locus_tag="OO564_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6654037.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(12951..13058)
                     /locus_tag="OO564_002990"
     CDS             complement(12951..13058)
                     /locus_tag="OO564_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654038.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13230..>13611)
                     /locus_tag="OO564_002991"
     CDS             complement(13230..>13611)
                     /locus_tag="OO564_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654039.1"
                     /translation="HPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGPPALYIA
                     MSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVE
                     TYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3498 a         3146 c         3371 g         3596 t
ORIGIN      
        1 taagcgttat cacgaattgc cggtcagcga attatggctg gtggatgtcg agggtggcaa
       61 agctaaactg gatattattt tcgatctctg ccaacggatg attgataacg ctggcgtccc
      121 gatgaagctt tataaaacgc tggattgccg cgaagcattg aaagatgctg atttcgttac
      181 tacccaactg cgcgttggcc aattaccggc gcgtgaactg gatgaacgta ttccattaag
      241 tcatggttat cttggtcagg aaaccaacgg cgcgggcggt ttgtttaaag gtctgcgtac
      301 cattccggtg atttttgaca tcgtaaaaga tgtcgaagaa ctttgtccga atgcatgggt
      361 gattaacttc actaacccgg cgggaatggt tactgaagcc gtttatcgtc ataccggatt
      421 taaacgcttt atcggcgtgt gtaatattcc gatcggcatg aagatgttta ttcacgatgt
      481 tctgatgctg aaagacagcg atgatttatc tatcgatttg tttggcctca accatatggt
      541 gttcattaag aatgtgctgg taaatggcaa gtcgcgcttt gccgaattgc ttgatggtgt
      601 ggcatccggg cagttaaaag catctggcgt taaaaatatt ttcgatctgc catttagcga
      661 aggcttaatt cgttctctga atctgttgcc gtgttcttat ttgctttatt acttcaagca
      721 aaaagagatg ctggctattg aaatgggcga atactacaaa ggcggcgcac gagcgcaggt
      781 agtacagaaa gtcgagaaac aactttttga gctgtataaa aacccggagt tgaaagttaa
      841 gccgaaagaa ctggaacagc gcggtggggc ttattactct gatgcagcgt gcgaagtgat
      901 caacgctatc tacaacgaca agcaagctga acattacgtt aatatcccgc atcatgggca
      961 tattgataat attccggcag actgggcggt agaaatgacc tgtaagctgg ggcgcgatgg
     1021 cgcgacgcca catccgcgca ttacgcattt cgatgataaa gtgatggggc tgattcacac
     1081 cattaaaggc ttcgagattg ctgccagcaa cgccgcactt agcggagaat ttaacgatgt
     1141 gttactggcg ctaaacctta gtccgttggt gcattccgat cgcgatgctg agctgctggc
     1201 acgcgagatg attctggcgc acgagaaatg gctgccaaat tttgccgact gcatcgcaga
     1261 gcttaaaaaa gcacattaac cgaggctgat tatggaacgc ttactgattg ttaatgccga
     1321 tgattttggc ttaagcaaag gtcagaacta cggcattatc gaggcctgtc gcaatgggat
     1381 tgtcacatcg acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag
     1441 tcgtgatgaa ccgagtctgg ccatagggat gcactttgtc cttactatgg gcaagccact
     1501 gacagctatg ccggggttaa cccgcgatgg tgtgctggga aaatggatct ggcagttggc
     1561 agaagaagat gctttaccgc tggaagaaat tactcaggag cttgccagtc agtatttgcg
     1621 tttcattgag ctatttggac gcaaacctac gcatcttgat agccatcatc atgtgcatat
     1681 gttcccgcag attttcccga ttgttgccag gtttgcggct gaagagggga ttgcgttgcg
     1741 catcgaccgt cagccgctat ctaacgctgg tgatttaccg gctaatctgc gcagttcgca
     1801 gggattcagt agcgcgtttt atggtgaaga gattagcgag gcactgttcc tgcaagtgct
     1861 ggatgacgca agccatcggg gtgatccttc gctggaggtg atgtgtcatc cggcgtttat
     1921 cgataatact atccgtcaga gtgcttactg tttcccacgg ttaacggagc tggaagtgct
     1981 gacatcagcg tcgttgaaat atgcaattgc tgagcgtggt tatcggttgg gtagttatct
     2041 tgatgtgtaa gtaaggtggt aaggtcactc cccaggggac gtgaccttat ccatatttac
     2101 gccggaattt tgtcaatctt aggaatgcgt gaccacacgc ggtgttctgc catcagcgtt
     2161 aacagttcat ccataaaact accgtcggtg ctgtcagctt ccacaatccc ttcttcaccc
     2221 tggtcagcta ccttgattgt tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt
     2281 ttaaggtgtt tgtaggcttc catcagatag tagttggcat cgccgttgtc agcgatatcc
     2341 gcgatattgc cgcaagggac aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag
     2401 gtagcggcta taggcaacac cgtaccgtca tccgcagtca cttcacccat tcgggagtag
     2461 agcagtttgg catgaacgtc tttggccttc agggccttga gaatggcaag aaggtctgcc
     2521 gatctcactt catcattaag taaaatcgct accacgcgac ctttcacatc accgtcagga
     2581 atggcgtaca aacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg
     2641 atattcagct ggtcgtcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga
     2701 gtgagatcaa tatgcgccag ctggtcaaca acgtgctcac gaatatacgg acgaacgact
     2761 ttgcttaact caaaactgaa accatcgaca atatggcgct gctcgaatgg cgtctgactt
     2821 agccagaaca gacgcggatg ggaataatat tcgccaaacg atgggctgcg ctcgcgaact
     2881 ttattgcctt ccacgcgctc ttggtatgat tcaaaaccgc cgcgtttcgg ccccggtggt
     2941 gtttcgcgcg gccagttatc gttgatcgag ttcggttcat aattcgccgg gttagtgtcg
     3001 atccccatac gatgcatgcc gtcacgctgg aaattatggt aagggcaggt cgggcggtta
     3061 atcggaatct catggaaatt cggcccacca agacgactga tttgtgtatc ggtataggag
     3121 aacaaacgtc cctgcaacag gggatcgttg gtgaagtcca gaccaggaac aatatgacct
     3181 ggatggaatg ctgcttgttc gttttcggca aagaagttat ccggattgcg attgagcacc
     3241 attttgccga cacgctgaac gggcaccagt tcttccggga tcagtttggt tggatcgaga
     3301 agatcgaagt cgaacttgaa ttcatcttct tcaggaatca actggaagcc cagttcgtat
     3361 tccggaaaat cgcctgcttc aatggcttcc cacaactcgc ggcggtggaa gtccgggtca
     3421 cgtccggtga gtttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc
     3481 cagtggaaac gtacaaacgt tgccttccct tcggcattaa tcaggcggaa ggtgtgaata
     3541 ccgaagcctt ccatggtgcg gtaactgcgg gggatgccgc gatccgacat cgcccacatc
     3601 acgttgtgca gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcactt
     3661 tgcccttgtg gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat
     3721 ttatgcgcat cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc
     3781 tcttcagtat agaacttggt ggcaaaacca cggatatcac gcacggtatc ggcagagcca
     3841 gcgccaccct gaacggtaga gaacctaaca aatactgggg tgattttgtt cggatctgag
     3901 aggaaatccg ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct
     3961 gctgatccgc gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgggtgatt
     4021 ttctcgcgca gaataaaatc ttccagcagc gttggaccac ggttaccggc acgcagtgag
     4081 ttttgatcat cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct
     4141 ttgcgtacgt cttccagaga attaagtttt tcgttacgtg tatcaggggc tttcaggctc
     4201 ccaggggcgg taggttgtgc acctggcggt gttggttcag ccgctggacg atgagatccg
     4261 tcctcaggtg ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac
     4321 tggtgctgat gtgggttctt ttcgttatgt tgcgacattg aactcgtctc cttaatttat
     4381 tactgaaagg gccgctgttt tggtcaaaaa actctctaac tatagaccac cagccagatt
     4441 ccgcttcgga gactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag
     4501 gggctaaatc ggctaaacta cagtttttct aatttttaat atgcgtttag tgaagccagt
     4561 aatgaagaaa ccgctccgcc agcaaaaccg ccagattatt agctatgtcc cacgcacgga
     4621 acccgcgccg ccagaacatg cgataaagat ggattcgttt cgtgatgtct ggatgctgcg
     4681 tggcaaatat gttgcgtttg tactgatggg agagtcattt ctgcgctcac cggcgtttac
     4741 tgtgcctgaa tctgcccaac gttgggcaaa tcagatccgc caggaagggg aagtgactga
     4801 gtaaggatta catacctcct ggttttgccg ggagttacag gacctgaaat tctgcatttc
     4861 gttgctacat tcattcaaac cacctgtaac aaaaagctgc ctttgacgag ctattgctat
     4921 aaataagtga tcttttttct ttaattgttt atttatttta aaatggttaa gttttgctaa
     4981 tcatgatgag ttatttttga tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa
     5041 atggcttaga tttaaataag ataataattt tgttaacaat atgaggcggg agatgttttc
     5101 tacaaactta atacagagta attatggtga tttaaatatt aaaagcctgg ctttcgactc
     5161 ttttaaagaa aggcttgagt ctaccatggc ggcactcact ttctttatat caacgggtca
     5221 atgtgactgc gatgaaatag cagagtcgaa cttcaatcgt atgattgcct atatgtctaa
     5281 cattaactat gatgcctcaa agctaggagc gccagcactt agttttgata cgtatctgca
     5341 ggacaatgtt aaatatagag tgataattaa taatctttat ggcagtgagt ttcggataag
     5401 agatattaat gaggattttg ttgggatgga ttttacctgt gaattccgac ccgaaaaaat
     5461 gacgaatttg atcaatataa aaaataggtt ggttattcat tatttaagga ctatatacta
     5521 tgaaaaaaat tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga
     5581 gtctcctaaa gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac
     5641 taaatcttcc gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt
     5701 cacttagaaa acgtttcggg tatgaggttt taagcttctc actacaggca cattcattga
     5761 gccaggaatg tattgataag ttggacattt ttttcgctga cgatctgttt aaatatgagt
     5821 cagtatgcat cgccgcgatg gaacatttaa aaagtaaagc aacggcccct atacaaaacg
     5881 gaccgttgcc agcataagaa ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga
     5941 atggctttat cggtttgctt cagccactgg ctggtcagcg tgccagctgt catcgagcca
     6001 ctaacgttta gcgccgtacg gcccatgtcg ataagcggtt caacggagat taacagcgcc
     6061 accagggtta ctggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca
     6121 ccaccgacac cggcaacgcc tgcggaacta acggtaacaa taccgaccag cgtcgcaatc
     6181 cacatcgggt ccagcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc
     6241 ggatacaaac cggcgcagcc gttctgacca atggttgcac cgaaagaggc ggcgaaactg
     6301 gcgatggatt caggaacgcc cagacgacgc gtttgtgctt ccacattcag tgggatagac
     6361 gcagcactgg aacggctggt aaaggcaaac gtcagcacag gccatacctt acggaagtac
     6421 ttcagcggac tcacgccatt aatgcccagc agaatgccat gcacagcaaa cataatcagc
     6481 agaccgaggt aggacgcgac aacgaaactt cccagtttga tgatgtcttg caagttagaa
     6541 cctgcaacca ctttggtcat tagagccaga acgccgtaag gggtcaactg catgacaagg
     6601 cgaaccagtt tcatcaccca gctttgtagg gtatcgatag cggttaagac gcgttcacct
     6661 ttcggcgcat catccttcag cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt
     6721 accacgctga taattgacgt cggattggct ccggtaagat cggcaaacgg gtttttcggg
     6781 ataaaggaca agaccagctg cggaacgctc agatcagaga ctttaccaac atagttactt
     6841 tcaatggcgt tcagacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa
     6901 ccaaacaggt ggtgaccagc acaccgacca gcgccgcaat cagcgtggta aacaaaagcg
     6961 taccgatggt cagaaaactg attttgccta actgagatgc gttatgcaga cgggcaaccg
     7021 cgctcagaat agaggcgaag actaacggca taacgatcat ttgcagcagt tgaacattgc
     7081 cgttaccaac gatgttaaac cactgtacag aatctttaag tacctggctg tcagaaccat
     7141 aaatggtatg cagggcaagg ccaaaaacca cacccatcac cagacccacc agcacttttt
     7201 tcgccagact ccactgttta tggcgggtct gagccagcgc aaacagcagt acaacgaaca
     7261 ccacgatgtt cgcaattaat ggaaagttca tccccgttct cctgatctta ttatttctat
     7321 cactgcgtag cagtcatagt gttggcgcaa gattaacaga actgtgagct ggctcttata
     7381 ttcaaatgga attgcttatt ccagaaaggt gatttttgcc tttttagtat tcaacctgat
     7441 gcttaataag ccgatgaaac tggatctgca aggtattgat catttgcgat gaccaacgaa
     7501 cagcgctgtt ctcgggtaat gaatggggca tccataccga ccagacaaat aatgccatgc
     7561 agataaaacg ttccagttgg ttgccctttt ggggaaccag gataggcaag cggaaacgcc
     7621 agcggcatgg ccagagcagg ggaacaccgg cgggtgtcag catatcggca agtatgtggc
     7681 tcaaataacc cagcaccatt ccttgtagcg catcagccgg aatgaaccag ccttccggaa
     7741 ccttaaggta aaaggttgcc agcagcgcaa ataccgccag cagactgtgg gtaaaaccac
     7801 gatgcccaaa agcgcgggcg atcggttttg atatccattt taatcgctgc ccaagaaacg
     7861 actttgggtg atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc
     7921 accagtcacc ctgtgccagc acgggcgtca gctcggcatt tttggcaaat accgcacaag
     7981 caatagaaaa gagaaggtga ccttccgccg tcatgataaa actcactaaa ctgtcgatac
     8041 gtacagtata ggatttttat acagtgcacg gaagtggtga cggtgtaact ctttctctta
     8101 aattgacgat taatccgtca gttgaatgca aattcagata aagaaccagg ggcagcgtga
     8161 tcactgcccc tggtcattaa ccgagaaggt cttttgcggt gagttctgtc agcgatgaga
     8221 gtttgacgtt tgctaataca aaacgtggat cattttgcgc ttctggcgca ggaacgacga
     8281 tggaacgcat gcaggctgct ttagaagcga tcatgccatt taccgagtct tccagcgcca
     8341 cgcaggtcag tgggtcaacg cccagttttg ctgcgcagtc gagatatact tgcggatgcg
     8401 gcttgctgta aggcagtttt tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa
     8461 acatggtcaa cactttttcc agcatatgta gtggtgacgc ggaggccagt cccaccaata
     8521 aaccttgttc tttgcataac gcaacagctt cgcgcacgcc tggtaataat ggacgtgtct
     8581 cttcaaccag tgaaatggca cgggcaataa cccgttctac tacttcctga cggcttggcc
     8641 cattccacgg ttgccgggcg tacaaagatc gaccaccata tcgatgcgta aacctaaggt
     8701 gtccggcagc tcgttacgac gggagatatc tacccccagg cttgccatca catccagttc
     8761 ggctcgatcc cataaaggtt ctgagtcgat aagtaatcca tccatatcaa aaattgcagc
     8821 aagaatctga cgcggggttg acattacaac ctctccttta gagcattatg tgtaaggtat
     8881 taagccgtgc ttatgattaa gcgcctatca tatcgccttt aaagaatggg cgaaaacaga
     8941 tatctacagg taaactaagt gaaacgacgc gtcttaatca aggagaactc atgacgtatc
     9001 aacaagctgg acgcattgct gttttgaaaa ggattttggg ttgggtgatt tttatacctg
     9061 cattgatctc tacattgatt tctttgctga aatttatgaa tacccggcag gaaaaccagg
     9121 aaggcattaa tgcggtcatg ctcgactttg ctcatgtcat gatcgatatg atgcaggcga
     9181 atacgccttt tttgaatctg ttctggtata actccccgac ccctaatttt aatggtggtg
     9241 tgaacgttat gttctgggtg attttcatcc tgatttttgt cggactggcg ttgcaggatt
     9301 ccggtgcccg gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa gatcaactca
     9361 ttctggaaaa agccaaaggg gaagaggggc taacgcgtga acaaattgag tcccgtattg
     9421 ttgttccgca ccatactatt ttcctgcagt ttttctcact atatatcctg ccggttattt
     9481 gtattgctgc aggttatgtg ttcttttctc tgcttgggtt tatttaatca aaaggccgca
     9541 cggagcggcc attatcctca acccatatca tgctgctaat aatctatcca atgactgctg
     9601 agcaataacc aaatgctgac cgccaaataa tcttgcacga tttaacagcg tgtagagttg
     9661 gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg gatactgact gatagccgtc
     9721 atagatttgt ggcggctgtt cagtatgcag cggtaacatc gccaggtcgc actctcggtc
     9781 accccagtag caggcagggt cgaaaatgta cgggccatcc ggacccagcg cacagttgcc
     9841 ggaccataaa tcgccgtgca acagagaagg ctgcggttga tgcgaggcca gacgctgctg
     9901 aatatgctcg acgatggcgt cgatattgcc gaaagcgatc cctttctctg ctgccagttc
     9961 caactgccag ccaatccgtt gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt
    10021 gggctgtgga gttgtggaga gcgagttatc aaaatcgagg ccaaattgtg gttggtcact
    10081 ccattgatgt aaacgcgcaa tttgctgacc aagaataaat gcgctatgcg catccagcgg
    10141 acgaggtggg agataatcca tcaccagaaa actgtagtca cggtcagcgc ctactgccca
    10201 aaccttaggc acggtgacgg ttttactacg cgacagtaac tccagttggt cggcttcggc
    10261 ggtaaaactg ggaagcagtt ccctttcatc acatttgacg aaaaagtcat gtcctgcata
    10321 gcgcaaatgc catgcggcat ggacttctcc gccaggcagt tcattacgca gttcgatttc
    10381 gccttcacct aactgctcgc tcaaaagacg actgattgcc tgccacatga tgtctctccc
    10441 atgttgtctg ccagatcata aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg
    10501 cacaccacag actgattatt gcccaaaaac catttatttt ctgttgctat tccattcctc
    10561 ccaggtcgtg atggtgattt caggatcttt gccggttgca ctttgggtaa gccccgaaac
    10621 cagttctcta atttcttctt cactttgggt actgataagt ccaaaagtgt tagtccccag
    10681 ctcatggata tttccctcat catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta
    10741 attattcaac tcgttaattt cagtcaatgt atcctcatgc aacatgacgg ttatgacata
    10801 acggacaaga tcgccactgg ccatagttca cccctttgtt atcacatgac ttttgagcat
    10861 agtcggagaa acgcgttgcc gacaggttgg tggtgattcc cccaatgctg ggggaatgtt
    10921 tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg
    10981 ttccctgatt gagtgtggct gcactccccg cagctacgcc aaaacggacc atctcttcaa
    11041 gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa
    11101 cggtactctg gcttttcacc ggtggtggca ccacctgaat gcagttttca ctatcaacac
    11161 ccagcgctcc ttgtggacca agggaaacga caacccgttt ggccttgccg ctattaacga
    11221 tttcctgcgc ggctttgcgg acatcgtctg gctgggtgag ttcgcgactc accagcgcac
    11281 tgagttcttt ttggttaggc ttaaccaact cgatgttacc aatcgccagt gctgcactta
    11341 acgcttcgcc agaactgtcg atgatgcagc ggatcccttg gttttgcgca gcggaaatca
    11401 gttgggttaa tttttccagc ttcacacctg gcggcaggct tccgcttatg accaggatgg
    11461 ccccggattc aatttccaga acttgctctt caagctggcg aaactcatct tcatttaatg
    11521 ccgcgcctgg cataacaaaa cgatactgtt caccgcttgc ttccacatgt acgtgtaaat
    11581 tctgccgggt ccagtctttg gcttctacag tagcgacggg gacattttca tccgccaaca
    11641 gtgaaaccag gtgttcgccg gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc
    11701 caagatgggc aatggcgcgg gcgacgttga tgccgccgcc cccgggttcg aacaccggtg
    11761 cggtacagcg cagttttcct tcgggataaa tttgcggggt aattgttgcg ctatcgagag
    11821 agggcgcaag tgtcaacgta tagatacgta ccatcatttc ctcctatagg ctgatttcag
    11881 tctggcaccg aatcagcgga aagtgaagaa attaacaata tgatttatta gagctttatt
    11941 taaaattttg cagataaata tatataaata aaaatctgtt ttcgccacat gcttattcaa
    12001 aaaatgaaat aagaatacat tgctaagtta ttcgcgcctt tttataattc gttacctttc
    12061 cagggacgct aatctttgat ccttaagaaa gtttccggga ccgtgatggt ctcttttttg
    12121 ttgtacggac tcttgataaa tgaagctttt gaagacagtt cctgccatcg ttatgttggc
    12181 ggggggcatg tttgcgtcac tgaatgcagc tgccgatgat tccgttttta ctgtcatgga
    12241 cgatcctgcc agcgctaaaa aacctttcga aggtaatctg aacgcaggat atcttgcaca
    12301 atcaggcaat acgaaaagct cctcacttac ggctgacacc accatgacct ggtatggaca
    12361 aaccactgcc tggtcgctgt gggggaacgc cagtaatact tcttctaatg atgagcgttc
    12421 ttcagaaaaa tatgcggcgg gcgggcgtag ccgctttaat ttaactgatt atgactattt
    12481 atttggtcag gcaagctggc tgacagaccg ttataacggc tatcgagagc gcgatgtgtt
    12541 gaccgcgggt tatggtcgtc agtttttgaa tggcccggta cacagtttcc gctttgaatt
    12601 cggtccaggt gtgcgttacg acaaatatac cgacaatgcc agtgaaaccc agccgctggg
    12661 ctatgcctcc ggggcttatg catggcagtt gactgacaac gctaaattta cgcagggtgt
    12721 ttcggtcttt ggcgcggaag acacaacgct gaactccgaa agcgcattaa atgttgctat
    12781 taatgaacat tttggactga aggtggctta caacgtcaca tggaactctg aacctccaga
    12841 gtctgcaccg gaacataccg accgtcggac aaccttatcg ctgggctaca gcatgtaaaa
    12901 ttatctgccg gaccgtgtta tgcggtctgg ctgtcttcgt ttgtcactgc tcagccccaa
    12961 atcattgctg cccaacgtag caacaacata gcgccacaga ttgccagtag caccaccacc
    13021 atcttccagt gttttttatc aaagcgttta gtcggcatac cagtgtcctg catatctctt
    13081 gtgcgtgata acggagtgta acaaaaggga caggcatcaa gggatttttt gttattacag
    13141 ccgtcgggtg gcgttgtgca catggtcact gtttctgcgt tttatgaaaa aaataaaatt
    13201 atgtatcgat atatatcgta aatagctggt tataaatacg tctttatttc atcagcacta
    13261 aaactcaggc tttggctgat gacatttata gcagcataat atgtttctac agttttataa
    13321 tgcttatgat gaatggctat atgaacagct gacatgttgt catgattttt agcggccaat
    13381 agtgtaaata actgatgttg actaaaagaa tgtttttttg caaaagcacc cagcgtcgat
    13441 atataagata acacgacgtc ttcattaccc ttgctcatgg cgatgtataa tgcagggggt
    13501 ccctgtgctg tagctccttt taataaatcc atgatgttag ctttgctcaa cttgtattta
    13561 aaggcgatgc cattaatttt agagaggaac cgtgtgacac acaaagggtg a
//
LOCUS       DAJTYI010000107        13492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYI010000107 DAJTYI010000000
VERSION     DAJTYI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..346
                     /locus_tag="OO564_002992"
                     /pseudo
     CDS             1..346
                     /locus_tag="OO564_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            406..513
                     /locus_tag="OO564_002993"
     CDS             406..513
                     /locus_tag="OO564_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6654040.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(494..1225)
                     /gene="yehW"
                     /locus_tag="OO564_002994"
     CDS             complement(494..1225)
                     /gene="yehW"
                     /locus_tag="OO564_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6654041.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1230..2156)
                     /gene="yehX"
                     /locus_tag="OO564_002995"
                     /pseudo
     CDS             complement(1230..2156)
                     /gene="yehX"
                     /locus_tag="OO564_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2149..3307)
                     /gene="yehY"
                     /locus_tag="OO564_002996"
                     /pseudo
     CDS             complement(2149..3307)
                     /gene="yehY"
                     /locus_tag="OO564_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3314..4231)
                     /gene="osmF"
                     /locus_tag="OO564_002997"
     CDS             complement(3314..4231)
                     /gene="osmF"
                     /locus_tag="OO564_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6654042.1"
                     /translation="MPLLKLWAGSLVMLAAVSLLLQATSPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4483..6780)
                     /gene="bglX"
                     /locus_tag="OO564_002998"
     CDS             complement(4483..6780)
                     /gene="bglX"
                     /locus_tag="OO564_002998"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6654043.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            6976..8691
                     /gene="dld"
                     /locus_tag="OO564_002999"
     CDS             6976..8691
                     /gene="dld"
                     /locus_tag="OO564_002999"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6654044.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8729..9661)
                     /gene="pbpG"
                     /locus_tag="OO564_003000"
     CDS             complement(8729..9661)
                     /gene="pbpG"
                     /locus_tag="OO564_003000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6654045.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9835..10422)
                     /locus_tag="OO564_003001"
     CDS             complement(9835..10422)
                     /locus_tag="OO564_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6654046.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHYYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10592..11170
                     /locus_tag="OO564_003002"
     CDS             10592..11170
                     /locus_tag="OO564_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6654047.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11287..12048)
                     /locus_tag="OO564_003003"
                     /pseudo
     CDS             complement(11287..12048)
                     /locus_tag="OO564_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12101..>13492)
                     /gene="mdtQ"
                     /locus_tag="OO564_003004"
     CDS             complement(12101..>13492)
                     /gene="mdtQ"
                     /locus_tag="OO564_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6654048.1"
                     /translation="LLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWW
                     LEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKM
                     SAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAA
                     EREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDG
                     METDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELG
                     YSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQ
                     MGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAE
                     KNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDAS
                     IRLTGALGGGYKR"
BASE COUNT         3186 a         3804 c         3414 g         3088 t
ORIGIN      
        1 caacaaccga ctctccaggc gctgctggcg atcctcgacg gcgtactgat caactacatc
       61 gccatttgtc tggcttcggc acgtaaaaaa cagggtaaag atgcgctggt ggttggctgg
      121 aatattcagg ataccacccg tctgtggctg gagggctgga ttgccagtca acaaggatgg
      181 cgcattgatg tcctcgctcg cccactcgct caatcaacta cgccctgaac tattcgaagg
      241 ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc gcccaacagc agcaactcac
      301 cagttggcaa gaacaaggcc atgatatttt cccactcggt atttaatgat tcgttaacaa
      361 atgcgcttta ctgtacaatc ctttcgttaa cataaggagt gcattatgcg catagctaaa
      421 attggggtca tcgccctgtt cctgtttatg gcgttaggcg gaattggtgg cgtcatgctc
      481 gcaggttata cctttatttt gcgtgctggc taagcgcctg caccagcctt tcaaacaggc
      541 ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc ctggatcaca tacgcggtat
      601 taaatccgct aagcccgatg atgatgggcg tacccagcgt actggcccct accgttgagg
      661 cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc agaatcaccg
      721 gagccgccag cggtagctcg accttacgca gtcgctgacc tcgactcatt cccatacctt
      781 tcgcaacttc tgtcacgctg ggatcaatcg ctcccagccc ggcaagtgtc acctgcagga
      841 tgggcagcac accgtaaagg atcaaggcga taatcgctgg ttgcagacca aagccgatca
      901 ccggaacggc aatcgccagc actgcgacag gcggaaaagt ctgtccaacg gcggcaatag
      961 tttccaccag tgggcgaaat tccgcgcccc acgggcgagt aacagcaatt ccggcacctg
     1021 tgccaatgat caccgcaaac aaactcgaaa ttcccaccag ccagaaatga gccagtgcca
     1081 gagctgcaaa actttcttgc tgataaacgg gtcgtggcag ttgtgggaac aaggcagcaa
     1141 acagcggctg gctgtaaggc agccagaaaa tcagcgccac aaaaagagca atgagccaga
     1201 acagcggatc gcgcaacatc ttcatacgct tacgcctcca ccagcagatc ctgaaaatgc
     1261 agcgtgccgc aaggctggcc ctgcgtgttc accaccggca gcacctcgca tccccgcgcg
     1321 acaaacaggg agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc accttctgcc
     1381 cgttcttcgc gacgcacgta atccgccacg ctacgtaacg aaagcaggtg cacccccagt
     1441 tcactacgtc caaaaaactg gcggacaaaa tcattagccg gacgagtcag catcgtcagc
     1501 ggattcccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc tgccagccgt
     1561 agcgcctcat caatatcatg agtgaccagc acaatagtgc gccccagcaa acggtgaatg
     1621 cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc aaaaggttca
     1681 tccatcagta agacttgcgg atcggcagcc agtgcacgcg ccactcccac acgttgctac
     1741 tgaccaccgg aaagctgatg cggataacgc tcacgcaaat ttgactccag ccccagtagc
     1801 gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg accatttttg taattgcggc
     1861 acggtagcga tgttttgcgc cacgctccag tgggggaaca ggccaataga ttgaatggca
     1921 tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc tccggcaaag
     1981 cggatcactc cgctgtcatg ctccaccagg cggttaatca ttttcagggt ggtggatttg
     2041 ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt gagattgaga
     2101 tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat ggctaaattc aatcattacg
     2161 tttcaccttc agcagtgcga taaacaaatc gaacagcgcg tcggtaagca ccgccagaac
     2221 aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca gcccctggaa
     2281 aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca tacctacagt
     2341 ttgcaccatc accacccgca ggctgcgcag aaataccggt aacgccagcg gtaactgaac
     2401 atgcaggaaa cgctgcgccc cgctcatccc catcgctctg gcgctctcca gcacatcgcg
     2461 cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg catagagcac
     2521 cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcttcc ccagccacgg
     2581 aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg ccaccgaagg
     2641 cacggtctga atgacattga gcagagaaaa aattgccccc tgccgcgcag tggaaaagta
     2701 gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac caaacagcaa
     2761 cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt attctttcat
     2821 ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata atggcaatct
     2881 gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg gcatcgctac
     2941 aggccagcaa tgccagcgcc gccgccagcc agaaaccact gccgaggctg gtacgcgcca
     3001 gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc agctccccac accaacaata
     3061 cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg gggacaaaac
     3121 aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc ggccacagtt
     3181 gccagagatg aagcccttca cccgagacta aacgattagg cgcgtaactg ataaacggca
     3241 gcgccgctgc gattgccgtc agcaacaaca gcagcgccag aacaggatta atacgtagat
     3301 aagtcacggg aaattacttc gcccaccctt tttgtttcag gtagtcggca gccacttttt
     3361 tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt tttgcatcga
     3421 ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactccttc aacaccgatt
     3481 cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc ttgcggatcg cttaaggttt
     3541 gcagccccag cgccgcgacc ggagcgtcag tgccgtaagc cattgcggca ttaacgccag
     3601 aggtttgctg ggcagcggct ttaatcgtca ccgccgtgtc gccaccagcc agtgacagca
     3661 actgatcctg accgagctta aagccatagg ctttttcaaa cgcgggtaac gcatcggcgc
     3721 gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctcttgc agataacgac
     3781 tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc acggcgatag
     3841 tccaggtgtt atttgcaggc gcgggcgtca gccagattaa cttgttgtgc tccgcatcga
     3901 gctttttgac tttctcgtag ccctgcccgg catttttcca cgctgcatcg ttttcatctt
     3961 taaagaagaa agcgccattg ccggtatatt ccggatagat atccagttca ccggaagtaa
     4021 tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc ggtacgccgt
     4081 ggctttcgag tacctgcaag atgatgttgc cgagcagcgc gccttcggta tcgatttttg
     4141 aaccgacttt aacgggggaa gtcgcctgta gcagcaggct cacggctgcc aacataacca
     4201 gtgaacctgc ccagagcttt aagagtggca tgatgctttc ctcattcttt tactgttgtt
     4261 ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga gcggaaggat
     4321 tcagttgcag aatcagataa atacttaaga ggcatattcg gtccggcgtt tcctgccgga
     4381 tgcggcgcga gcgccttatc cggcctacaa agggcgaaaa ttcagcaagt taagatatga
     4441 acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca actcaaactc
     4501 gcctttctta acgcgtgcgg aatcagtgcc gataaataca ttgaacttgc caggctcggc
     4561 gtcatatttc atctgttgat tccagaactt cagcgcctca atatcgatcg ggaagctgac
     4621 agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct gtttcacagg
     4681 gcgactcatg gaagccgtca catcctgcaa gtacatctgc actaccgtgg ccccctcgcg
     4741 cttaccggtg ttcgtcacct gcacgctggc cgtcactttg ccgtcacgct tcatggtcgg
     4801 cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat agccgaacgg
     4861 ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg gcttgtcggc
     4921 attatacgga cgaccggtgt tcagatggct gtagtacacc gggatctgcc cgacagaacg
     4981 cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata catcggcaat
     5041 tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag cctgctgatc
     5101 ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg gtttaccggt
     5161 ggctttcagc gccgcaatca agtcacgttg gccttgcgga atagtgatat cggtccggct
     5221 ggaggcttcg tgcgccatcc cctgtgcttc accgactaca gccaccacca catcagattg
     5281 tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gaacgcggat cgactttgac
     5341 cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa cgttcgcccc
     5401 tttggcatac agcactttac cgttttcacc gacggaattt ttaatcccgg tcagtacggt
     5461 cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac gtttactgtc
     5521 cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg tttcgagacg
     5581 gtttttcagc aacactaggc tttcgcgcgc cacttcacgc gcttctttac ggtgcaggcg
     5641 gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc tgtatgggtc
     5701 gttaaacaac cccatatcat atttaacgtt cagtacatgg cgggcagcat cgtccagctc
     5761 tgccatggtc actttgccgg atttaatcaa cccaggcaga tacttcgagt agtactcgtc
     5821 gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt ccgggtctgc
     5881 cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg tgatgccttt
     5941 aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg tggctggcgt
     6001 gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca gccccgcttt
     6061 gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg tgttgtactc
     6121 tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca tcaccgagta
     6181 acgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca ttattgaggt
     6241 gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat cgcgcgagac
     6301 atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat aagcagagac
     6361 acgcccgacc gttttcaccg catcgaggtt aaaagacgag gccagaccga ggctaatcgg
     6421 gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa ttttcaggcg
     6481 gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta cggtgttgaa
     6541 aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg gattatccgg
     6601 gcctacgctg attaagcgca gctgaccaat tttctcatca accatcattt tcttgagcag
     6661 atcggtgacg aacgcatccc gcgcttcagg cgttaatgga tggttaccga ataaatcatc
     6721 cgccagtgct ggctgcagga ccagactcac cgcgattcct actgaacata gccatttcat
     6781 atggattttt cttcctttgt tgcccgacgt ggcagcgaaa atggtgcaaa aaccgtagtt
     6841 tgccataagc atgatggaga gagaaaaaga atactcagtt tattgtctga attttcaaaa
     6901 tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt ttttcgccac
     6961 cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt tttgaatgaa
     7021 cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac ggcccgctat
     7081 cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc tggctcacta
     7141 ctggaattgt ggcgggtgct gaaagcctgc gtcaccgccg acaaaattat tctgatgcag
     7201 gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta tgatcgcgat
     7261 atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttctcggcaa gggcgaacag
     7321 gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa accgctggga
     7381 cgcgaaccgc attcggtgat tggatcatcg tgtataggcg catcggtcat cggcggtatt
     7441 tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga aatgtcgtta
     7501 ttcgcgcgta tagatgaaga tggcaaactg acgctggtga accatctggg gattgatctg
     7561 ggcgaaacgc cggagcagat ccttagcaag ctggatgacg atcgcatcaa agatgacgat
     7621 gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt tcgtgatatt
     7681 gaagccgaca cgcccgcacg ttataacgcc gatccggatc ggttatttga atcttctggt
     7741 tgcgccggga agctggccgt ctttgcggta cgtcttgata ccttcgaagc ggaaaaaaat
     7801 cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat ccgccgtcat
     7861 attctggcta acttcgaaaa tctgccggtt gccggggaat atatgcaccg ggatatctac
     7921 gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt aggcaccgac
     7981 aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga gaaagtgaaa
     8041 ttcttccgtc cgcattttac tgaccgtgca atgcaaaaat tcggtcacct gttccccagc
     8101 catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct gctgttaaaa
     8161 atggcaggcg atggcgtcgg tgaagccaaa tcgtggctgg tggattattt caaacaggcc
     8221 gagggcgatt tctttgtctg tacgccggag gaaggtagca aagcgttttt acaccgtttc
     8281 gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt cgaagacatt
     8341 ctggcactgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca tttaccgccg
     8401 gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg ctatgtcttc
     8461 catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttaaaaga acagatgctg
     8521 gaactgctac agcagcgcgg cgcgcagtac cctgccgagc ataacgtcgg tcatttgtat
     8581 aaagcaccgg agacgttgca gaagttctat cgcgagaacg atccgaccaa tagcatgaat
     8641 ccggggatcg gtaaaaccag taaacggaaa aactggcagg aagtggagta aaaattacgg
     8701 atggcagagt atcgccatcc gaattcactt aatcgttctg tgccgtctgc cccgccgccg
     8761 ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgccggcaca ggcatcactt
     8821 taccggtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa
     8881 acgcgtccat cactaccagc gccaccggtt tattattgat aaccgtacgc atcaccagac
     8941 aatggcccgc cgcattggta aagccggttt tggttaactg aatattccag ttatcgcgat
     9001 acaccagatg attagtattg cggaacggca gcgtatacgt cgggttagag aaggttgcca
     9061 tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg gcaatgagca
     9121 gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta ggttcaacaa
     9181 agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta ataaaggctt
     9241 tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc gcggttttct gaagacatca
     9301 gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg cgcgaataga
     9361 cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc
     9421 gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca
     9481 gatccgggtg gttcgaatag atcactttgt tggtattcag atcaacaatc atcgcgctac
     9541 cggaggcaat ttccggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg
     9601 gtgcaaaagg cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca
     9661 tgatgagcat tcagatagtg gttcacgcgc acgggttgcg caccgccgga gtaaggattt
     9721 actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag gagaatcgtg
     9781 agtaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa ctttctagaa
     9841 caaacgataa ccgtagcccc atagtataac ggttagggtg agcagcactt ccagtaccag
     9901 cacgccaatc gccagcgtcg aactggagaa gctaaggcct tcctctttgt tgatattcag
     9961 gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca ccgtgccgac
    10021 cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa acagcggagt
    10081 tgcaacgtag cccgcgaaga ccatgcagtg cgcaagagac ggacgctgcg ggtaattgcg
    10141 cgccatccac cagatgaccc gccccatcac cgcaacccca gccagcataa cgccgtaaaa
    10201 caagacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa
    10261 attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag
    10321 caaaacgtgg tgggtgtaat agtgagaaat cgtttcgttt tcgcgattaa tcacctgcat
    10381 ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga
    10441 gttcatgaac ctgacagttc aagtataagc cagcttgtga ttattttttg ttcatccatg
    10501 taatttgcac cgtctgatgt atgtcctttt tcagcgtgaa tgattcacag ggtgtatgct
    10561 tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca tctcacaata
    10621 tggttatgcc gcgctggtta tcggtagcct agcggaaggt gaaaccgtga ctttgctggg
    10681 aggcgttgcg gcacatcagg ggctattaaa gttcccactg gtggtacttt ctgtggcgct
    10741 tggtggcatg attggcgacc aggtactcta tctgtgcggg cggcggtttg gcggcaagct
    10801 gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg
    10861 ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg tgattggccc
    10921 gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg
    10981 cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc aggtgattgc
    11041 gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga ttctggtcgt
    11101 ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa agccggataa
    11161 tcaggcgtaa agcagttgcc acagataagc attcactcca catccggcac cgggtgttcc
    11221 atgcctgatg cgacgctggt gtcttatctg gcctaaccca ttgaaatatc cacgcacctc
    11281 actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa
    11341 cgactgccct gtcgtgtaat ttgcgccctc cgaacaaagc cacaccacca ggctggcaat
    11401 ctcatgcgtt gcgccaaaac gccgtagagg aatcgaaggc tccgcgtcgg gcttcacgtc
    11461 gctatcatcc atgctgttca ttggcgtggc tatcgcccca ggcgcgactg cgttcaccaa
    11521 aatcttatgc ctgaccaact ccagcgccat cgttttggtt aacccgccga gcgcatgttt
    11581 agcggctgtg taggcgctgg catccggcag cggcgtatgt tcatgtaccg acgtaatgtt
    11641 gatgatgcga ccgccctgcc cttatttcac catctgacga gccgcaattt gcgagcataa
    11701 aaatgcacca tcaacatcaa cggtaaaaat cttgcgccac tcatcaaaag ccatatcaag
    11761 aaacggcgct ttggtcattg cacccgcatt attcaccagc acatcaatac gcccaaatcg
    11821 ctggatgagt gtctccagcg cctgcgcacc ttctggcaga ttgccgagat ccagttgcac
    11881 gatctccgca cgtacgccgt gggtaaccac ctcacgcgcg gtatcttttg ccccttcttc
    11941 atctgagtgc caggtaatgc caatatcaaa cccctgatgc gccagtaata acgcgcactc
    12001 tttgccgatc cccgaatcgg aagcggtaat tatcgcgacc tatgccatcg agttctccac
    12061 ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc
    12121 ccagcgcacc ggtgagtcga atggaggcat ccagccactg cccttgcaat aacaggccat
    12181 tggcccgctc acgcagcgcc gggattctgg cttcgctgac gcgggaacca gcaatgatgc
    12241 ccgcgttaaa gcgggcctgc gcaagaccta ccacacgcaa ggcatcgcgc tcaatttgcg
    12301 cctgatgctg gtttttctcc gccagtgtct gaacctgact ggctgcccgc gccacgtcat
    12361 tcactgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg
    12421 cgatatcgag attggcgtta agacgaccac tatcgaaaat aggtagcgtc aggcctgccg
    12481 taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt
    12541 gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatagtgc
    12601 ttaacgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg gccagtaagg
    12661 agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc ggtttaagct
    12721 tcaatgactt agtctggtga tttgtgagtg catttaaccg tgcctcaata attttcattt
    12781 tccccacgac atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccatac
    12841 cgtcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg ataatggtgt
    12901 tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc
    12961 agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgccc
    13021 gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc cagatatcga
    13081 gatgccagcc cgccgttaag ccaaaagttc cgttggtgta ccacggaccg gtcgtacctg
    13141 cggccggatc gttcagagca aacggcccca ttagcccttc tgccgacatt ttttgccgct
    13201 ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc ttcgcctggg
    13261 cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc tgtagtgcat
    13321 tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg ctgtctggcc
    13381 aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc gtttgctggc
    13441 ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt aa
//
LOCUS       DAJTYI010000108        13376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYI010000108 DAJTYI010000000
VERSION     DAJTYI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..888)
                     /locus_tag="OO564_003005"
                     /pseudo
     CDS             complement(1..888)
                     /locus_tag="OO564_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1129..1929
                     /locus_tag="OO564_003006"
     CDS             1129..1929
                     /locus_tag="OO564_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6654049.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2007..2777
                     /locus_tag="OO564_003007"
     CDS             2007..2777
                     /locus_tag="OO564_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6654050.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2825..4183)
                     /gene="mltD"
                     /locus_tag="OO564_003008"
     CDS             complement(2825..4183)
                     /gene="mltD"
                     /locus_tag="OO564_003008"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6654051.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4255..5010)
                     /gene="gloB"
                     /locus_tag="OO564_003009"
     CDS             complement(4255..5010)
                     /gene="gloB"
                     /locus_tag="OO564_003009"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6654052.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5044..5766
                     /locus_tag="OO564_003010"
     CDS             5044..5766
                     /locus_tag="OO564_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6654053.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5763..6230)
                     /gene="rnhA"
                     /locus_tag="OO564_003011"
     CDS             complement(5763..6230)
                     /gene="rnhA"
                     /locus_tag="OO564_003011"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6654054.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6295..7026
                     /gene="dnaQ"
                     /locus_tag="OO564_003012"
     CDS             6295..7026
                     /gene="dnaQ"
                     /locus_tag="OO564_003012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6654055.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7159..7235
                     /locus_tag="OO564_003013"
     tRNA            7159..7235
                     /locus_tag="OO564_003013"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7193..7195,aa:Asp,seq:gtc)
     gene            complement(7366..8412)
                     /locus_tag="OO564_003014"
     CDS             complement(7366..8412)
                     /locus_tag="OO564_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6654056.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8423..9418)
                     /gene="tssG"
                     /locus_tag="OO564_003015"
                     /pseudo
     CDS             complement(8423..9418)
                     /gene="tssG"
                     /locus_tag="OO564_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9415..11298)
                     /gene="tssF"
                     /locus_tag="OO564_003016"
     CDS             complement(9415..11298)
                     /gene="tssF"
                     /locus_tag="OO564_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6654057.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11314..11808)
                     /gene="tssE"
                     /locus_tag="OO564_003017"
     CDS             complement(11314..11808)
                     /gene="tssE"
                     /locus_tag="OO564_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6654058.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11805..12635)
                     /locus_tag="OO564_003018"
     CDS             complement(11805..12635)
                     /locus_tag="OO564_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR6654059.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12622..13376)
                     /gene="tagK"
                     /locus_tag="OO564_003019"
                     /pseudo
     CDS             complement(12622..13376)
                     /gene="tagK"
                     /locus_tag="OO564_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3061 a         3489 c         3575 g         3251 t
ORIGIN      
        1 ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta cttaccgcac
       61 ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta tctgccatta
      121 actccaccaa ttatccgcga gcgatttctt tgtcgatcat gtagtcggac aaacacgcaa
      181 tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat gacaaaccgt
      241 acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg agggaagcgg
      301 gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct ggcttcccgt
      361 agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat aacggcctgg
      421 cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact tttctttcga
      481 tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga tagcgttcac
      541 ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg gcatcgatcc
      601 gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc gctgccatct
      661 cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc aggctaagtt
      721 gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt ttcaccgccc
      781 ggcttaccgc tgagtttgct tgccctaaaa ttgttccgct gcccggctaa agctgccgct
      841 ttcgacgacc gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt ttgctcctgt
      901 tgcaaaatag atgagatatt ttgaatttat ttgtcattaa accatcagga tgcgtgatat
      961 gtcatccgat ttaatgttct caataatgag caaatttctg accggtgtaa gcacttgctt
     1021 acataacaat atacaattgc tcgttgaaag agtgagctaa aatccctata acagtagaac
     1081 cctcccgagt gcggaagggt tgacgtaata gaggtttcaa agtcaaaagt gcgaaaaaac
     1141 acctatgcca tgcgctatgt tgccggacaa cctgcggaaa ggatcttacc gccggggtct
     1201 tttgcgagca tcggccaggc attaccacct ggagaaccgt taagtaccga agagcgtatt
     1261 cggatcctgg tgtggaacat atacaaacag caacgcgctg aatggttgtc ggtattaaag
     1321 aactacggca aagatgcaca tctggtgtta ttgcaggaag cgcagacaac gccagagtta
     1381 gtacagtttg cgaccgctaa ctatcttgcc gccgatcagg tgcccgcttt cgtgctgcca
     1441 caacatcctt ccggtgtaat gaccctttcg gcggcgcatc cagtgtattg ctgcccgtta
     1501 cgtgaacgag aacccatttt gcgtctggcg aagtcggcac tggtgacggt ctatccatta
     1561 cctgacaccc gcctgttgat ggtggttaat atacacgccg tcaacttcag tctgggcgtg
     1621 gatgtctata gtaagcagtt acttcctatt ggtgatcaga tagctcacca cagcggcccg
     1681 gtcattatgg cgggagattt caatgcctgg agccgtagaa ggatgaacgc gttatatcgc
     1741 tttgcgcggg aaatgtcgct gcgccaggtg cgttttaccg atgatcagcg ccgtcgggcg
     1801 tttggccgcc cgctcgattt tgttttctac cgtggtctga acgtcagcga agcttctgta
     1861 ttggttacgc gcgcttccga tcacaatccg ctactcgttg aattcagtcc cggcaagcct
     1921 gataaataag gtatgtcagg tctgccacag ggcagaccaa cgtttggcgc tgtcctttta
     1981 tactcaaaca acaaaaggac agcgcaatga caacacaatc ccaccatgac cacgtagaaa
     2041 agcagtttag ctctcaagcc tgcgaatatc tgaccagtac cgtacatgca tccggacgag
     2101 atttacagcg cctggcggtg cgtctggctg attatcctgg tgcaagtgta cttgatatgg
     2161 gctgcggagc agggcatgcc agctttgttg ctgcgcaaaa cgtgagcgcg gtggtggcgt
     2221 atgacttatc tgcccaaatg ctggatgtcg tggcacaagc tgccgaagcc cggcaactga
     2281 aaaatatcac cacccgccag ggatatgctg aaagtctgcc atttgccgat aacgcatttg
     2341 atattgttat cagccgttat tctgcccatc actggcatga tgttggtgca gcactgcgtg
     2401 aagtgaaccg gatattgaaa cctggcggta ggatgattgt gatggacgta atgtctccgg
     2461 gtcatccagt gcgcgacatc tggttacaga cggtagaagc attacgcgat acctctcacg
     2521 tacgaaacta cgccagcggt gagtggttga ggttaatcaa tgaagccaat ctgatagttg
     2581 ataatttaat tacagataag ttaccgctgg aattttgttc atgggtcgcg agaatgcgta
     2641 cgccagaagc gttagtagac gctattcgca tttaccaaca gagcgcatcg acagaggtga
     2701 gaacgtattt tgccttgcag aatgacggct ctttcaccag tgatatcatc atggtagaag
     2761 cacataaagc ggcataaata aaaaaggcac cgggggaatc ggtacctttt tattatctgg
     2821 tttgtcagga atctggcatg ttgttgtttt tcacaaacaa cgtcagcttg tcgcctggtt
     2881 gcagattcgc agtgtcgctg ttccagcgca tcacatcttt gatgttcacg ccgtggcgtt
     2941 tagcaatgct tgaaagcgaa tcgcctttgc gcacacgata cgtaatgcta tcgctgttgt
     3001 ttgccagtcg ctgtgcgcta ctgcctgcac caatcgtcaa actttggcct ggcttcagct
     3061 tagatccgcg cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag
     3121 cgatgcttga aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat
     3181 tgtcggcaac cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca
     3241 gttgatctgc atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg
     3301 agcctttcac gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg
     3361 caaccttcgc catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat
     3421 cggtcgttgg cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca
     3481 gcattttagg cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg
     3541 atttcccacg cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat
     3601 aagccgctac ggtcagaagc cagtcgccgt caaacatttt gttcagacgc tgcatcatat
     3661 tcagcgcggc agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt
     3721 tcaaaccata attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc
     3781 cagacgttgc gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag
     3841 gcatgttacg ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag
     3901 ttacatcgtg gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt
     3961 cattttccgg aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg
     4021 gcgcgataga cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc
     4081 cttgaccagc tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac
     4141 ccacgagcag gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg
     4201 cttaaaagac gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa
     4261 cctatctttc tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc
     4321 ttcattaatt acattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg
     4381 ctcatttttc agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt
     4441 acgataataa tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt
     4501 tgataacgta tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt
     4561 tttaagtgat tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga
     4621 aaacagcgtg tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt
     4681 gtgacccggt gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc
     4741 tttgactacc tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ccacaatttg
     4801 cggaaacttt tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag
     4861 aaatatggcc tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc
     4921 tccgggatcg acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc
     4981 atcaaaggcg ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt
     5041 gtgatgaaac cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg
     5101 ccctggggag agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa
     5161 atgtatggtt ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg
     5221 tgcgcggttt ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg
     5281 gatccacttc atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc
     5341 ttgccgtggt gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat
     5401 gatggctggc tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt
     5461 gtgccggtat tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa
     5521 ctggactggc tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc
     5581 ccgtggaaca aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta
     5641 caacttattg ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt
     5701 aaaaacaagc cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag
     5761 gcttaaactt cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga
     5821 gccagttcat cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat
     5881 ttgatttgat gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact
     5941 ggttttttgt ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata
     6001 ccctggcgga catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc
     6061 tccagcgcga caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct
     6121 gcgctaaagg ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca
     6181 ggattaccca gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga
     6241 cttcctgtaa ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc
     6301 actgcaatta cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt
     6361 gcacactatg aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc
     6421 ctgacgggca ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc
     6481 tttggcgtac atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta
     6541 gccgatgagt ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc
     6601 gatataggct ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat
     6661 accttctgta aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc
     6721 aacagcctcg atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac
     6781 ggggcattac tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa
     6841 acgtcgatgg cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt
     6901 cagcgcattg tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg
     6961 gcagcacatg aagcccgtct cgatctggtg cagaagaaag gcggaagctg cctctggcgg
     7021 gcataaatac atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga
     7081 ttcaaaagat gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt
     7141 cccaacggaa cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca
     7201 gggggtcgcg ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa
     7261 tgattcgggc tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat
     7321 atgatttaaa aaatggccag acattataaa ttctggctac ttctgttact ccagcgcatc
     7381 ccatttctcc agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac
     7441 caggcgttct gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa
     7501 ccagcgacgg atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg
     7561 ttctgcgccg gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc
     7621 tgcctgcatc tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg
     7681 tggcaggatc tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt
     7741 cagacgggag aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg
     7801 ctcagtggtc tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag
     7861 gtctgccagt tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga
     7921 tagcgaacgc tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat
     7981 gccacgaata tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg
     8041 tgccaccgca gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc
     8101 gtccgggtac gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc
     8161 accggcctgc tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg
     8221 catcagcaga cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact
     8281 gacaaaatcg ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta
     8341 ttccggatcg tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag
     8401 gaatatttcc atttaattat cctcagtgcg ccaggtagtg ttctggttcg aaaatcatgc
     8461 ccgtcaccgg gttgtcgtgc atggctttac ccgcccatgc agtccaaccc agttggtacg
     8521 gaccaccggt aacagccgga ggggcggcgt ggggctttag ctgtagctca atttcccagc
     8581 agtattcata accactaaag gcgcggacca gttctgtcag gacgggcagg ttattgctgc
     8641 caggcagaaa acgtcggtaa tcctccagcg tcagcgggcc gataaccagc cggaacttat
     8701 actgcatgtc cggcacggcc tggccgatga gtgcactatt gccaataacg gaagaggggg
     8761 caggcatccc cagccgggtg atctcatcgt ccgccacggc tatccagtgc aggacatact
     8821 cctgtatccg gaaaggtaca ccgaaatagt gcgccagcgt ggtggccaga ccgtccggat
     8881 tgcgggactc acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc
     8941 tgtcagcgct ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt
     9001 catgttgcgg tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa
     9061 actgcgtcag ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggatcgcgtc
     9121 ggctttcagt ccggttacgc gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc
     9181 cccacagccc gaggctgtac agactgatgt gcaggcgccc ttcctgccag cccacactgg
     9241 cgatttctcg tggcgcaaag gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc
     9301 gtggttgcta ggtgttgccc gccggaacgg tacacaactg cacatccacg cggcgcatca
     9361 ggttcagaaa accgtaatgc caggggggtt cctgcgcctg agtgaaatcg ttgctcatac
     9421 gcttccccgc tgtcccgccc tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg
     9481 cagcgtcatc tgcgagaagg tattaatgga aacatggcgg gcgatatagt gttccagcac
     9541 caggccgaac agatacggac tgatgccgga aaaaccttct tcatccaccg tcagttcaca
     9601 actgacgcca cgcccataga ccagtagtcc ggaaccgggc agacggcggg tgaccggggt
     9661 cgttttacaa ccaatcaggc tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat
     9721 aaacaggttc agcagatctc gcaacgcctg gccgccggta cggtgatcca gatcggccag
     9781 cggcagataa ttaaaagata actggcggat caaccgccat gccatctcac gttcagccag
     9841 cgggggctgc ggcgagcgtg gcgggcggat aagccctacg cccgccaccg ggatggctgc
     9901 atccacggtt agatcgtccc ggccattgcg ggcaataaga cagggcagat cgcggttagt
     9961 gaccatggcc gtgatggtga tgtggcgcag attttccggg tacggcgctt catactggtc
    10021 aaccagcgac aggaacactt ccgagcccgt atagggggta cgggtgccgt aacggcgggc
    10081 gttttctgat aagcggcggg gctcccggcg cagggaaaaa taacgtccgt gattgccttc
    10141 atcattattg cgggtgtggt agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc
    10201 cagcccttcc acttcctgga ccgagaacac ctcataatcc agcggatggg ttctgtccac
    10261 caccagatgc tgttccgtcg tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa
    10321 caggttgata accggggtac agaacaggct gaactgtgcc gcatttgtct ggtggatcag
    10381 ccagtccggc ggcaggcggt tcagcaggat aacaatctcc gccacgccac cgtcaatttt
    10441 ctgtaatccg gcagacaggc ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa
    10501 atattcatgc agcaggttat gaccgtgaaa cacgttccac gccagcggca gcagcccctg
    10561 atccggctcc aggccttcgt acataacggg ctgttgcaga ttcacatcca gcgcgccgtc
    10621 aaaatgcccc ggaatgccag ccagggtcgc gatggcgctg gtatgtagaa gttctaacag
    10681 gtgtgaggcc gtacgctcct cgccgcagag ataaaacggc agcctgtcca gtccggcaag
    10741 ctgactgaac gttagttcac caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg
    10801 aatgttggcc ggcaggtagc ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg
    10861 cgcctcttcg atggcgagcg gccacagcgt tacatcctgg ctgctgcgaa actggcaggc
    10921 agtgctttcg ccttccggaa tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc
    10981 tttcgccaga tcgccttctt ccgtatcggg atgcaactgc gccacgctca ttgagggcgt
    11041 gggtgtgacg taattggggc tgaccacttc cagcgggcgc tgggtgaaac gggggaattc
    11101 agcgtcaatt tttagctgcg tgcgggcggt caggaagctg aaggcctcga ccattcgctc
    11161 tacatacgga tcggcaatat ccgttccctg catgcccagc cgggcggcga ctttgggatg
    11221 acgggcggca aattcggctc ccgtttcccg cagatagctc agttcgcggt tgtagtatcc
    11281 cagtagccgt ggatccatcg gtctctctct gaattaaaaa aacgtaatgc gactctgctc
    11341 catatccagc gcgctacgta cccggaatgc agtcggatac ggctgcgtga ggatctcgcc
    11401 gcgaatttca aactgtaatg tgttatggcc actctggcgg cgctcatcct gaagtgggat
    11461 gatttgcagg gtggacgctt tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc
    11521 cctgcggata tcgtcccact tgtgctcata cagaaagctg cccgccagtg gcggcaggcc
    11581 gtagttaagt acggacgctg ccgcgtacgg ataacgtgca gcatcaatat catcttcatg
    11641 gctgacggtg ttgagcagaa atgacaaatc ccggcggatg atctccttaa tctgaaccgg
    11701 gctgacgctg ataccgtggt cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa
    11761 cagcgtgggc agcaggtgat tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct
    11821 tcagcggtgt taaaggtaca ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg
    11881 agccacaccc tctgacccag cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc
    11941 gtttcccggc acagacgcag ggagtcggca gccttttcag agccgctgta gcggggaaac
    12001 agccaggcac tgtgtgtatc gccattgttc agggtgacgt tgacgggttt ccagatcaaa
    12061 tcgatcagat gcgcaggccg cggcgatttc agcgaacaga tttgtgaaaa cggcagccag
    12121 atatatgctc cgcccgcgat cagttcgagt accggaccgg tacgggagtc gctgtcgcta
    12181 atccagtcaa atgcgccgcc attccactgc ccggaagtgt ctgttatggc ctcaagcgcg
    12241 cggctgcggt gagcatcagc ttcctgcgct tcaccacggg cgttacaggc gagggtggtc
    12301 agcagatcct ccatccaggc gggcggcggg agaataacac cgggccgctg ttcgccctga
    12361 aagcaggcgt ggcggtatat ctcacagcgg atcagctcgc caaatacctg cgcttcgcga
    12421 gtataatttg cgtccatccg ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg
    12481 cctgccacgc acaataactg aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc
    12541 ctgacctgtt gctctgccat ctgaatgccc tccaccacgg aataatcccg cagcagcgcc
    12601 gacaaggtaa tcggtagctc gttattgttt gtcatgatat atctctccat gttgtgccgg
    12661 gtttatccgg taatggttgt caatgccaat gatgcgatgc tctttccgtg tcagatctgg
    12721 cagtacggtg ttatgcgctg tttccggggc gtattcgggg gaaagtaaat gcaggatatc
    12781 agggggagtc ttttcgacag gccagatagt ttcatcttcg ccagtggcat ccagtacatt
    12841 gagaatagcg tcaatgctta acccaccagt gaccatatcc tgcaatgtct cggcttcggt
    12901 gaccaggtca cgcaatgaat ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg
    12961 atcagcatgg cgattaagat cttgttcggg agataacaac gcctcgcggt attcctgata
    13021 aagtcgggtc agggcatttc ctttttccgc accttctccg gtggaggagg ctccatgaac
    13081 aggaatgata tcgaatggat tttgtgatgt cagatgctgc tgcctgaacc aatccagatc
    13141 cagatattcg gttattgctt ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct
    13201 caggtccgga gatgatggat tgccatgtac aaggcactat gaagataacc cccattctat
    13261 caggtcgcct tcattcagac gcatacggtg atgaggttcc acaatctggt cgtttaccgt
    13321 acagcaatat tcggtagact gattgataat ccaccatgct tcttcgtggc gggtca
//
LOCUS       DAJTYI010000109        13186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYI010000109 DAJTYI010000000
VERSION     DAJTYI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..494)
                     /locus_tag="OO564_003020"
     CDS             complement(<1..494)
                     /locus_tag="OO564_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6654060.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(526..1218)
                     /gene="fimC"
                     /locus_tag="OO564_003021"
     CDS             complement(526..1218)
                     /gene="fimC"
                     /locus_tag="OO564_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6654061.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1419..1961)
                     /gene="fimA"
                     /locus_tag="OO564_003022"
     CDS             complement(1419..1961)
                     /gene="fimA"
                     /locus_tag="OO564_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6654062.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2442..3308
                     /gene="folD"
                     /locus_tag="OO564_003023"
     CDS             2442..3308
                     /gene="folD"
                     /locus_tag="OO564_003023"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6654063.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3310..3522
                     /gene="ybcJ"
                     /locus_tag="OO564_003024"
     CDS             3310..3522
                     /gene="ybcJ"
                     /locus_tag="OO564_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6654064.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3630..4151
                     /locus_tag="OO564_003025"
     CDS             3630..4151
                     /locus_tag="OO564_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6654065.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4187..5572)
                     /gene="cysS"
                     /locus_tag="OO564_003026"
     CDS             complement(4187..5572)
                     /gene="cysS"
                     /locus_tag="OO564_003026"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6654066.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5746..6240
                     /gene="ppiB"
                     /locus_tag="OO564_003027"
     CDS             5746..6240
                     /gene="ppiB"
                     /locus_tag="OO564_003027"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6654067.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6243..6965
                     /gene="lpxH"
                     /locus_tag="OO564_003028"
     CDS             6243..6965
                     /gene="lpxH"
                     /locus_tag="OO564_003028"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6654068.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7083..7592
                     /gene="purE"
                     /locus_tag="OO564_003029"
     CDS             7083..7592
                     /gene="purE"
                     /locus_tag="OO564_003029"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6654069.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7589..8656
                     /gene="purK"
                     /locus_tag="OO564_003030"
     CDS             7589..8656
                     /gene="purK"
                     /locus_tag="OO564_003030"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6654070.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8793..9686)
                     /locus_tag="OO564_003031"
     CDS             complement(8793..9686)
                     /locus_tag="OO564_003031"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6654071.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9683..10330)
                     /locus_tag="OO564_003032"
                     /pseudo
     CDS             complement(9683..10330)
                     /locus_tag="OO564_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10333..11565
                     /gene="allC"
                     /locus_tag="OO564_003033"
     CDS             10333..11565
                     /gene="allC"
                     /locus_tag="OO564_003033"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6654072.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11576..12361
                     /gene="allE"
                     /locus_tag="OO564_003034"
     CDS             11576..12361
                     /gene="allE"
                     /locus_tag="OO564_003034"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6654073.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12589..>13186)
                     /locus_tag="OO564_003035"
     CDS             complement(12589..>13186)
                     /locus_tag="OO564_003035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR6654074.1"
                     /translation="ANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEMVEELEQGLQ
                     NYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAVNLAQAVQGA
                     ALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVHQYGIDALFS
                     ILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
BASE COUNT         3183 a         3448 c         3330 g         3225 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattta gctctgatat tgtcagatga ttcggttagt ttaagttcac
      541 ttctctgacc ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg
      601 cactcctgag ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa
      661 cttttggctg cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac
      721 attgatatag ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc
      781 ttgtaattga attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac
      841 attcctgccg ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa
      901 cagagactca cgatctgccg ccagcggtgg accggtgtaa ataatacgca aagtattttc
      961 gcttttgggt tcactaacaa atagtggcgg tgtaatgatg aatgactttt cttttacacc
     1021 gctgctgttt tcaatccacg aattgaccag aaagagctca ttggtatggc tatttttaat
     1081 ccataccgca gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat
     1141 ccctccggca gcatgggcgc tggccgaaat gattaaataa aatatcatga gcatcaataa
     1201 ctttatttta gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aagattgctt
     1261 tggcttctta caacacatca ttcccttttc atttaataaa gcggcatgat acaaagtgaa
     1321 cgtggtttcg atttacattg ccaccatacc gcttcagaca accggtacat tactttgtta
     1381 taagacgtga tatataattc aaaacaacgt ggttttgatt attcgtatct cataataaaa
     1441 gtcgcgtcag catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca
     1501 gaaaaatgaa gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca
     1561 tcaggtttga gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt
     1621 gttgtatttg tactggatgc aatggccaat aaattatcat tgattgcatc agcctgacca
     1681 gaaaatgcaa cggcagcatt agcggcaaca acaggatcgc agtcgttcaa ctgaatggtg
     1741 aatggaatta atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg
     1801 agtgtgacaa cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg
     1861 ccttcaaagt gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat
     1921 agtgcggcag ccagcgcaga cgaaataaat cttaatttca tatatattcc ttcaatctca
     1981 tttatcgact ccacatccgt atataaccga ttactttatt taagacactg atagtagtaa
     2041 aattcctttt tatcctctaa gaatgtctta attgaaaata tgcactctat tctaaaaaat
     2101 agagagcccc gttagatgaa tacttccgcg caaaatatat tcaacacaaa tatagacctg
     2161 aagcggtaaa ttaccaggct gaaaattctt tttatattgt caggtatttc ttaaattatc
     2221 ttaatcctta aacaaggaaa taaatcagtt ccagatttac aacgccatca tgtacgaaaa
     2281 atgaagcttt caatctcagc gacctcagcg acggtgcgcc taaccttcgc aagaggtcgc
     2341 ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc cgcttctgac aaaatagacg
     2401 catccccttc gacctacgta acagatggaa tcctctctct gatggcagca aagattattg
     2461 acggtaaaac gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt caggctcgta
     2521 ttgcagccgg actgcgggca ccaggactgg ccgttgtgct ggtgggtagt aaccctgcat
     2581 cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga agtcgggttc gtctcccgct
     2641 cttatgacct cccggaaacc accagcgaag cggagctgct ggagcttatc gatgcgctga
     2701 atgccgacaa caccatcgat ggcattctgg ttcaactgcc gttaccggcg ggtattgata
     2761 acgtcaaagt gctggaacgt attcatccgg acaaagacgt ggacggtttc catccttaca
     2821 acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg cgcggtatcg
     2881 tcacgctgct tgagcgttac aacattgata ccttcggcct caacgccgtg gtgattggcg
     2941 catcgaatat cgtcggtcgc ccaatgagca tggaactgtt gctcgcaggt tgcaccacca
     3001 ccgtgactca ccgcttcact aaaaatctgc gtcatcacgt agaaaatgcc gatctgttga
     3061 tcgttgccgt tggcaagcca ggctttattc ccggtgactg gatcaaagaa ggcgcaattg
     3121 tgattgatgt cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac gtcgtgtttg
     3181 aagacgcggc taaacgcgcc tcatacatta cgcctgttcc cggcggcgtt ggcccgatga
     3241 cggttgccac gctgattgaa aacacgctac aggcgtgcgt tgaatatcat gatccacagg
     3301 gtgagtaaca tggcgacatt ttctttaggt aaacatccgc acgttgagct gtgcgacttg
     3361 ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat tgccgaaggc
     3421 caggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct gcaaaatcgt cgccggtcag
     3481 acagtgagtt ttgcaggaca cagcgtacag gttgttgcct gatccctttc ccggcctgta
     3541 tcaacaggcc ggttctcttt tcagactttt gccctctacg ccccgttcat ccatgctgat
     3601 agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt tattacgcac gccgccgttc
     3661 ccctttgcat tggtttaggg ttgggttcga aagtcatccc tccgcgtctg ttatttgccg
     3721 gaatcatcct agcgatgctg cccgatgccg acgtattgtc gtttaaattt ggcgttgctt
     3781 acggcaatgt ttttggtcat cgcgggttta cccattcgct ggtgtttgcg tttgttgtcc
     3841 cgctcttatg tgtgctgatt ggacgtcgat ggttcagggc tgggctgatt cgctgctggc
     3901 tgtttttaac cgtctcgttg ctatcgcaca gcttactgga ttcggtaacc actggcggta
     3961 agggcgttgg ctggctgtgg ccgtggtcag atgaacgctt tttcgctccc tggcaggtga
     4021 ttaaagtcgc gccgtttgca ttgtctcgtt acaccacagc gtacgggcat caggtgatta
     4081 tttccgaatt gatgtgggtg tggttgccgg ggatgctact gatgggaatg ttgtggtggc
     4141 gcagacgata accggatgcg aaaactcgca tccggcaatt gcgcaattac ttacgacgcc
     4201 aggtggtccc ttgcgggcca tcttccagca cgatccccat ctcgttaaga cggtcacgtg
     4261 ccgcatctgc cgccgcccag tctttcgctt tacgggcatc cagacgctgt tgaattaacg
     4321 cttcaatctc agccacttcg ctgtcgtctg cctgcgcgcc gctttgcagg aacgcttccg
     4381 gttcttgctc cagcaggccc aatacagcgg aaagtttacg caggtgagat gccattgcat
     4441 tcgccgctgc catatcttct gctttcagac ggttcacttc acgcgccata tcaaacagca
     4501 cggaataggc ttccggggtg ttgaaatcgt cgtccatcgc ttcaataaag cgcgcttcaa
     4561 acgcttcgcc accggcaggc gcaacggttt tatctgtgcc gcgcagcgca gtgtagaggc
     4621 gctccagcgc cgcacgcgcc tgcttcaggt tctcttcgct gtagttcagc tggctgcgat
     4681 agtggcccga catcaggaag taacgcacgg tttccgcgtc gtagtatttc agcacatcgc
     4741 gcacggtaaa gaagttaccc agcgatttgg acatcttctc gcggtcaacc atcaccattc
     4801 cggagtgcat ccagtagttc acatactgac catcatgggc acaggtggac tgcgcgattt
     4861 cgttctcgtg gtgcgggaac atcaggtctg aaccgccgcc gtggatatca aagtggttac
     4921 ccagctgctt gcagttcatg gccgaacatt caatgtgcca gccaggacga cccgcgcccc
     4981 acggagacgg ccagctcggt tcgccctctt tcgacatctt ccacagaacg aagtccatcg
     5041 ggttgcgttt gtcatcaacc acgtcaacgc gcgcgccagc ctgcagctgg tcgagatcct
     5101 gacgcgacag cacgccataa gttggatcgg tcgggacgtc gaacatcacg tcgccgttgt
     5161 ccgccacata agcgtgacct ttggcgatca gttgttcagt gagttcaata atttctgcga
     5221 tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat gttcaaagca tcaaaatctt
     5281 tgtgcatttc ggcaatcatg cgatccacca gcgccacaaa gctttcgcca ttttcattgg
     5341 cgcgtttgat gattttgtcg tcgatatcgg taatgttgcg cacatacttc agtttatagc
     5401 cgaggaaacg cagatagcgc gcaaccacgt caaaagcaac aaaggtacgc ccgtgaccga
     5461 tatgacagag atcgtaaacg gtgattccac acacgtacat gccgacttcc ccggcgtgaa
     5521 taggcttaaa ttcctctttt tggcgtgtca gagtattgaa gatttttagc atcgaagatt
     5581 ccgtttagac atgtgtgggt aattgagttg cgtataatac ccatatttcc cgcgcgaatc
     5641 agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc ctatatatga
     5701 cccgaactgg gttgaagcac caatcaaacg gaacaggatg caaaaatggt tactttccac
     5761 accaatcacg gcgatattgt catcaaaact tttgacaata aagcacctga aacagttaaa
     5821 aacttcctgg actactgccg cgaaggtttt tacaacaaca ccattttcca ccgtgttatc
     5881 aacggcttta tgattcaggg cggcggtttt gaacctggca tgaaacaaaa agccaccaaa
     5941 gaaccgatca aaaacgaagc caacaacggc ctgaaaaata cccgtggtac gctggcaatg
     6001 gcacgtactc aggctccgca ctctgcaact gcacagttct tcatcaacgt ggttgataac
     6061 gacttcctga acttctctgg cgaaagcctg caaggttggg gctactgcgt gtttgctgaa
     6121 gtggttgacg gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg tcgtaacggt
     6181 atgcaccagg acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt tagcgagtaa
     6241 tcgtggcgac actctttatt gcagatcttc atctctgcgt ggaagaaccg gcgatcaccg
     6301 ccggttttct gcgtttttta gcgggggaag cccgcaaggc cgacgcgctg tatattcttg
     6361 gcgatctgtt tgaagcatgg attggcgacg acgatcccaa cccactccat cgccagatgg
     6421 cggcagcgat caaagcggtg tccgattccg gcgttccctg ttatttcatt catggcaacc
     6481 gtgattttct gctcggcaaa cgctttgccc gtgaaagcgg catgacatta ttgccggaag
     6541 aaaaggtgct cgaactttat ggtcgccggg tgttgattat gcatggcgac acgctgtgca
     6601 ccgatgacgc gggttatcag gcttttcgcg ccaaagtcca caaaccctgg ctgcaaatgc
     6661 tattcctcgc cctgccgttg tttgtgcgca aacgcattgc cgcgcgaatg cgcgcgaaca
     6721 gcaaagaagc caacagcagt aaatcgctgg cgatcatgga cgttaaccaa aacgctgtgg
     6781 tcagtgcgat ggaaaaacat caggtgcaat ggctaatcca cgggcatacc catcgcccgg
     6841 cggtgcatga acttatcgcc aaccagcaac ctgcttttcg cgtggtactg ggtgcctggc
     6901 atacggaagg ttcgatggtg aaagtcacgg cggatgacgt tgagctgatt cattttccgt
     6961 tttaaaaaac ccacaacttt gctgatttca cagccacgca accgttttcc ttgctctctt
     7021 tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg caccacagga gttttaagac
     7081 gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc aaaagcgact
     7141 gggctaccat gcagttcgcc gtcgaaatct tcgaaatcct gaatgtcccg caccacgttg
     7201 aagtggtttc tgctcaccgc acccccgata aactgttcag cttcgccgaa agcgccgaag
     7261 agaacggtta tcaggtgatt attgcgggcg caggcggcgc agcgcacctg ccaggcatga
     7321 ttgccgccaa aacgctggtg ccggtgctgg gcgtgccagt acagagcgcc gcactgagcg
     7381 gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg cattccggtg ggtacgctgg
     7441 cgattggtaa agctggcgcg gcaaacgcgg cattactggc agcacaaatt ctcgcgactc
     7501 acgataaaga gctacaccag cgtctgaatg gctggcgcaa agcccagacc gacgaagtgc
     7561 tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg gtaacgggca
     7621 gttaggccgt atgctgcgtc aggcaggcga accgttaggc attgctgtct ggccggtcgg
     7681 gctggacgct gaaccggcgg cggtgccttt tcaacaaagc gtgattaccg ctgagatcga
     7741 acgctggccg gaaaccgcat taacccgcga gctggcgcgt catccggcct ttgtgaaccg
     7801 cgatgtgttc ccgattattg ctgaccgtct gactcagaaa cagcttttcg ataagctcca
     7861 cctgccgacc gcgccgtggc agttacttgc cgatcgcagc gagtggcctg cggtgtttga
     7921 tcgtttaggt gaactggcga ttgttaagcg tcgcactggt ggctatgacg gtcgcggtca
     7981 atggagttta cgcgccaatg aaaccgaaca gttaccggca gagtgttacg gtgaatgtat
     8041 tgtcgagcag ggcattaact tctctggtga agtatcgctg gttggcgcgc gcggctttga
     8101 tggcagcacc gtgttttatc cgctgacgca taacctgcat caggacggta ttttgcgcac
     8161 cagcgtcgct tttccgcagg ccaacgcaca gcagcaggcg caagccgaag agatgctgtc
     8221 ggcgattatg caggagctgg gctatgtggg cgtgatggcg atggagtgtt ttgtcacccc
     8281 gcaaggtctg ctgatcaacg aactggctcc gcgtgtgcat aacagcggtc actggacaca
     8341 aaacggtgcc agcatcagcc agtttgagct gcatctgcgg gcgattaccg atctgccgtt
     8401 accgcaacca gtagtgaata atccgtcggt gatgatcaat ctgattggta gcgatgtgaa
     8461 ttatgactgg ctgaaattgc cgctggtgca tctgcactgg tacgacaaag aagtccgtcc
     8521 ggggcgtaaa gtggggcatc tgaatttgac cgacagcgac acatcgcgtc tgactgcgac
     8581 gctggaagcc ttaatcccgc tgctgccgcc agagtatgcc agcggcgtga tttgggcgca
     8641 gagtaagttc agttaagcag gtgctcattt gattgccgga tgcggcgtaa acgccttatc
     8701 cggcttaccg ctccggtacc cattgtaggc ctgataagac gcgtcaagcg tcgcatcagg
     8761 catggtgcac cgattgccgg atgctctcat cactacagcg aaatacaggt ccccgcttcg
     8821 cccgccagcg tctcttcaat tcgcgataac gccccaatcc acgcgggttt actacggctt
     8881 ctgacataac cactcaccgc cgttaccttc ggccccatcg aaccatcggc tttggcaaat
     8941 ggcgctaact catccggtgt ggcatggcga atggcacgtt gctgcggcgt tccccagttt
     9001 tcatataccg catcagcatc ggtgaggatc accagtccat cggcattaat ctgctcggcg
     9061 agcaacgcag cggcgagatc tttatcaatc accgcttcac tccctgcccc atcttccgtc
     9121 acaggcacac cgccgccgcc actgcaaatc accacatgcc cctctttgag caacaactcg
     9181 atggcttcgc tgtcgagaat tttacgcggt tgcggagacg ccaccaccca gcgcaaatat
     9241 ttaccatcac gtttcatctg ccagccgtaa gccgcttcca gtgcctcttg ttcttctggc
     9301 tgataaaccg gaccaataaa tttctctggc tgcaaaaacg ccggatcatc aggcgaaacc
     9361 tcaatgcgcg tcagcaccgt cgtaacaggc ggcatctgcg gctgtgcgct caagctctgc
     9421 gccagcatat agccaatcat cccctggctt tccgccacca gcacatccag cggatacggt
     9481 tctacctctt tccacgccag attctgtaac gccagcagcc ccacctgcgg cccgttgccg
     9541 tgaacaatcg ccagccgata agaacgggcc aggcgtgcca gcgcgggtac agcactggcg
     9601 atattgcgat attgattttc cgccgtcagc gcctcaccgc gctggagtaa ggcgttgccc
     9661 ccaagagcta caaccagtgt tttcatggtt ttccttgtaa taattgttgg ccaagccaga
     9721 accccagcag cgtatctgcc cctgacgtat gccccagcgc cagcagcgaa tcaatcgcaa
     9781 cagcggtacg tttcgggcaa ctcagagcat ggacaaagtg caggagtggc gaggcgaaat
     9841 atccttgcgc ggcataacgt aaataactga cgctcaccgc tgtggtaacg agttgaagat
     9901 tgtcggaaca ggcaaaaaac gggcgaccgg cgcgcgcatc taaagcgcca taataccagg
     9961 ccgccagcag cattccgctc aacgtgtcat catgacttgg cgttaatccg gggcctttac
    10021 ccagccagtg ccgccagtcg gtcttaacgc cattgagcgc ggcctgaaaa cagtgacgaa
    10081 actggcgtaa ttcagcgggc agcggatcgc ttgccgccat cgccagtggc ccgaaaagtc
    10141 cggtttcctc cgcgcgttgc atccatgcag ctgcaagcgg ttgaggatgc gaaggcggcg
    10201 taatacgcag caaacaataa cgctgtggct gtttaaccgt gaaacgcccg aggcgaatcc
    10261 cttgagccac aacctgtggt cgttcattgc cgcataatcc accgcataac gcatcgaatt
    10321 gcgtgcggcg aaattacaca ttcccgtcaa gctatagaag aaacgctgcc ctggctttcc
    10381 tcttttggcg ctgacccaac gggtgggatg acccgtttac tttattcgcc ggaatggctg
    10441 gaaacccagc agcaatttaa aaaaagaatg gcagcaagtg ggctggaaac acgtttcgat
    10501 gaagtgggga atttatacgg tcgcctgagt ggcaccgaat atccacagga agtggttctg
    10561 agcggttcgc atatcgatac cgtggttaac ggcggtaacc tcgacgggca attcggcgcg
    10621 ctggcggcgt ggctggcaat tgactggttg aaaacgcaat acggcgcacc gttacgtacg
    10681 gtcgaagtgg tggcgatggc agaagaagaa ggcagccgct tcccgtatgt cttctggggc
    10741 agtaaaaata tctttgggct ggcgaatcct gacgacgtgc ggaatatctg tgatgccaaa
    10801 ggaaatagtt ttgccgatgc gatgaaggct tgcggattta ctctgcctaa cgccccacta
    10861 actccgcgtc aggatataaa agcctttgtt gaactgcata ttgaacaggg ctgtgtgctg
    10921 gaaagtaatg ggcaatcaat tggcgtggtg aatgcaattg tcgggcagcg tcgctatacg
    10981 gtgacgctga acggcgagtc aaaccatgca ggcaccacgc cgatgggtta tcgtcgtgat
    11041 acggtttacg ctttcagtcg catttgccat cagtcgatcg aaaaagcgaa aaagatgggc
    11101 gatccgctgg ttctgacctt tggcaaagta gagccgcgcc cgaatacggt aaatgtggtg
    11161 ccgggtaaaa ccacgttcac cattgattgt cgtcataccg acgctgccgt gctgcgcgat
    11221 ttcacccaac agttagaaaa cgacatgcgg gcgatttgcg atgaaatgga cattggtatt
    11281 gatatcgatt tatggatgga cgaagaaccc gtgccgatga ataaggagct ggtcgccacc
    11341 ctgacagaat tgtgtgaaag agaaaaactg aattaccggg tgatgcacag tggtgccggg
    11401 cacgacgcgc aaattttcgc gcctcgcgta ccaacctgca tgatttttat ccccagcatc
    11461 aacgggatca gccataaccc ggcggaacgc accaatatta ctgaccttgc cgaaggggtc
    11521 aaaacgttgg cactcatgct ttatcaactt gcctggcaga aataaggagt cataaatggg
    11581 atatttaaat aacgtcaccg gttaccgcga agatttactg gctaaccgtg cgattgttaa
    11641 acacggtaat ttcgcactgt taaccccgga tggtctggta aaaaatatta ttccgggctt
    11701 tgaaaattgt gacgcgacaa tcctctccac gccaaagctg ggtgcctctt ttgttgatta
    11761 tctggtcaca ctgcatcaaa acggtggcaa ccaacagggc ttcggtggcg aaggcattga
    11821 aacgttcctg tatgtgatct ctggaaatat cactgccaaa gccgaaggca aaacatttgc
    11881 cttaagcgaa ggtggctatc tttattgccc accaggctcc ttaatgacgt ttgttaacgc
    11941 ccaggccgaa gacagccaaa tctttttata taagcgccgc tatgttccgg tagaaggcca
    12001 tgcaccgtgg ctggtttctg gcaatgccag cgaactggaa cgcattcact atgaaggtat
    12061 ggatgatgtg atcctgctgg attttctgcc caaagagtta ggttttgata tgaacatgca
    12121 tatcctctct tttgcaccag gtgccagcca cggttatatc gaaacacacg ttcaggaaca
    12181 tggtgcctat attctttccg gtcagggggt ttataacctc gacaataact ggatcccggt
    12241 gaaaaaaggc gattacatct ttatgggcgc ttattcttta caggctggtt atggcgtagg
    12301 gcgtggtgaa gcgttcagct atatttactc gaaagattgc aaccgcgacg tagagattta
    12361 agtcatacca ctcgcctgat tatttttagc tatgttggat tttgccacgg tttataccgt
    12421 ggcttttttt gtaacattgc ctgatgcgct tcgcttatca ggcctacatg acctctgcaa
    12481 tatatagaat ttgcgcgatt ttcaggaagg atatagtact catgccgcat ccggcatgag
    12541 tactgcgctc ttatacgaaa taaatcgcat ccgctttaaa ggcaagagtc agtttttaat
    12601 tccctgacct attttaatgg cgcaggcaat atttcgcgcg ctgttgaaga gattggtttc
    12661 accgctggcg agcacttcgg ctaaaggtgc cagacgaggc aaaatgctga atagcgcgtc
    12721 aatgccgtac tggtgcacca cttccacgcc atcacccaat acaccagcaa tcccaatcac
    12781 cggtacatta aactgcttcg ccaccgacgc aacgcccagc ggcgctttac cgcctgccgt
    12841 ttgcgagtca atgcgccctt ccccggtgat caccagtgct gccccctgca ccgcctgcgc
    12901 aagattgacc gcattcaaca caatctcaat gcccggttta atatccgcat tgagaaatac
    12961 cgccgccgca atccccatac cgcccgcagc gccgccgccc gccatctgac agacattaat
    13021 ttcagtttgc tgttgtaaaa cacgggcgta attttgcagc ccctgttcca gctcttcaac
    13081 catctccggc gttgcccctt tttgcgggcc aaataccgcc gccgcgccgc gtgcccctac
    13141 cagcgggtta tcaacgtcac atgccacttc aatatggctg ttcgcc
//
LOCUS       DAJTYI010000110        12815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYI010000110 DAJTYI010000000
VERSION     DAJTYI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..151
                     /locus_tag="OO564_003036"
     CDS             <1..151
                     /locus_tag="OO564_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6654075.1"
                     /translation="LVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNL
                     YASMA"
     gene            308..1057
                     /locus_tag="OO564_003037"
     CDS             308..1057
                     /locus_tag="OO564_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6654076.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1079..1645
                     /gene="yieF"
                     /locus_tag="OO564_003038"
                     /gene_synonym="chrR"
     CDS             1079..1645
                     /gene="yieF"
                     /locus_tag="OO564_003038"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6654077.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1699..3036)
                     /gene="adeP"
                     /locus_tag="OO564_003039"
     CDS             complement(1699..3036)
                     /gene="adeP"
                     /locus_tag="OO564_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6654078.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3201..3866
                     /gene="yieH"
                     /locus_tag="OO564_003040"
     CDS             3201..3866
                     /gene="yieH"
                     /locus_tag="OO564_003040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6654079.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3933..4406
                     /locus_tag="OO564_003041"
     CDS             3933..4406
                     /locus_tag="OO564_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6654080.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4455..5042
                     /locus_tag="OO564_003042"
     CDS             4455..5042
                     /locus_tag="OO564_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6654081.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5104..5826)
                     /locus_tag="OO564_003043"
     CDS             complement(5104..5826)
                     /locus_tag="OO564_003043"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6654082.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(5828..7003)
                     /locus_tag="OO564_003044"
     CDS             complement(5828..7003)
                     /locus_tag="OO564_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6654083.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7030..8646)
                     /gene="bglH"
                     /locus_tag="OO564_003045"
     CDS             complement(7030..8646)
                     /gene="bglH"
                     /locus_tag="OO564_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6654084.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8732..10129)
                     /gene="bglB"
                     /locus_tag="OO564_003046"
     CDS             complement(8732..10129)
                     /gene="bglB"
                     /locus_tag="OO564_003046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6654085.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10145..12022)
                     /gene="bglF"
                     /locus_tag="OO564_003047"
     CDS             complement(10145..12022)
                     /gene="bglF"
                     /locus_tag="OO564_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6654086.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADVPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12156..>12815)
                     /gene="bglG"
                     /locus_tag="OO564_003048"
     CDS             complement(12156..>12815)
                     /gene="bglG"
                     /locus_tag="OO564_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6654087.1"
                     /translation="RLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHCQ
                     FAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHL
                     VSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEH
                     ASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKE
                     H"
BASE COUNT         3378 a         3231 c         2955 g         3251 t
ORIGIN      
        1 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
       61 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
      121 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
      181 acaataaaat gtaaaaaagt tgtaataagc ttgtctgaat cgaactttta gccgctttag
      241 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
      301 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
      361 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
      421 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
      481 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
      541 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
      601 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
      661 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
      721 cgaagcgcgg gcgcagctca ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
      781 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
      841 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
      901 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
      961 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     1021 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     1081 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     1141 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     1201 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     1261 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     1321 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     1381 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     1441 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     1501 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg
     1561 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     1621 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     1681 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     1741 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     1801 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     1861 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     1921 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     1981 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     2041 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     2101 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     2161 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     2221 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     2281 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     2341 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     2401 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa
     2461 aaagccgagg atacccagaa gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     2521 ttccgagtta gcgacaatca cacctgcgtt tttcagcccc atcatgccga taaacaggcc
     2581 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     2641 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     2701 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     2761 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     2821 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     2881 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     2941 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaacgcgtt ccagcatcct
     3001 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     3061 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     3121 attggattta cttatacggc aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa
     3181 actgattaaa aaggaaaggc atgtcccgga tagaagcggt atttttcgac tgcgacggta
     3241 cgctggtcga cagtgaagtc atttgctccc gcgcatatgt aacgatgttt caggaatttg
     3301 gtattacgct cgatcctgaa gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa
     3361 ttatcgatat tgtttccctt gaacatggtg ttacgttagc gaaaacagaa gctgaacacg
     3421 tttaccgtgc agaagtcgct cggctgttcg attcagaact ggaagccatc gaaggggctg
     3481 gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt atctaacggc ccaaataaca
     3541 aaatgcagca ttctatgggc aagctgaata tgttgcacta cttcccggat aaactgttca
     3601 gcggctacga tattcagcgc tggaagccag acccggcgtt aatgttccat gcggcaaaag
     3661 cgatgaatgt aaatgtagaa aactgcattc tggttgatga ctcagttgcc ggtgcgcaat
     3721 ctggtatcga cgcaggtatg gaagtgttct acttctgcgc cgacccgcac aataagccga
     3781 tcgttcaccc gaaagtcacc acctttaccc atctttcgca gttaccggaa ctgtggaaag
     3841 cgcgtggttg ggatattacg gcatagttct tcacactccc ttcacttacc ccgcttaaat
     3901 tggcgctcaa aggtaagtaa agggagtttg atatgtctgt ttcacgtcgg gtaatacatc
     3961 acggacttta ttttgcggtt ttaggaccgt taattggtgt tctgtttctt gtcctctaca
     4021 tattcttcgc aaaagaaccg ctggttcttt tggtaataat acaggtactt ccattgtttc
     4081 tcttattgtc gattactacg ggagctattc ctgcattgtt aaccggcgtg atggtcgcct
     4141 gtctgccgga aaagatcggg tcacagaaaa attatcgttg tctggctggt ggcataggtg
     4201 gcgtcgttat caccgagatc tattgtgcag ttattgtaca tattaagggc atggcttcct
     4261 cggagttgtt tgaaaacatt ctttctggtg acagtctcgt tgtccgcatc attcctgcat
     4321 tgctggcagg tgtggtgatg agcagaatca ttacccgtct gcccggattg gatatttcat
     4381 gtcctgaaac agactcttta agttaagcgg gatactttat ctttgggcta ctcaaaagca
     4441 gacaggatgt tactatgact caaaatatca ggccgttacc ccaattcaaa tatcatccca
     4501 agccactgga aacaggcgca tttgaacagg ataaaaccgt agagtgcgat tgctgtgaac
     4561 aacagacgtc agtttattac tcgggtccct tttattgcgt tgatgaagtt gaacatctct
     4621 gtccgttgtg tattgcggac ggttctgctg ctgaaaagtt tgcaggtagt tttcaggatg
     4681 atgccagcat agaaggtgtt gaatttgagt atgatgaaga ggacgaattt gccggtatta
     4741 agaacacata tcctgatgaa atgctgaaag agttggttga acgcacgcca ggttatcatg
     4801 gatggcaaca ggaattctgg cttgcgcatt gtggcgattt ctgtgctttt atcggctatg
     4861 tgggctggaa tgatataaaa gatcgcctcg atgaatttgc caaccttgaa gaagattgtg
     4921 agaatttcgg tattagaaat tctgatctag ctaaatgcct gcaaaagggt ggtgattgtc
     4981 agggttatct cttccgctgt ctccactgcg gcaagctgag actgtggggt gatttttcgt
     5041 agttatttaa ataatgagaa caggccggag cgtaattcat acatccggcc ttatttcttc
     5101 agcttaaccg aacgctaatt ctgcggcagc ggctttatca gcgattacca tcagcgaagg
     5161 atgcagctgc aacacggaag caggaacatc ttcggtaacc ggaccttgca gcatattttt
     5221 cagtgcctgc gctttacctg cgccactgac aattatcagc aagtttttcg ccgccatgat
     5281 gcttttcggc cccatcgtca cgtagctgtc aggcactaac gaaaagtcgc cgcccaactc
     5341 accatgcgcc acaatatcaa ccatctctcc caggatcggg aactccacgg tttgctcatg
     5401 gaaatgggtc gtattcggca aattgccaca aaaatgacca tctgcaccta atcccagcac
     5461 caccaaatcc aggccgcctt cacgcgccag tttctgatca tgctcgcggt agttatcaat
     5521 agtgagcttc tggatattct cttctttgat ccccgcaggg gtgaaaaaca gattacgcag
     5581 attggtaatc gttacgcctt ctccctcttt gccgcgaaag ggaatttcat cgaaattata
     5641 gaaatagcag ttatcgtacc aaggcttacc tttaaccagg gtggtgaggt attcatacat
     5701 gcctttgggc gtgctaccgg cggtaattgc caggttaaca cgacgcgtct tcgacatata
     5761 accaagcaga tggtgtgccg cgacacggct catttcctgg taatcttcgg taatgattaa
     5821 tttcatctta atgccttatt ttaaatttct gtacgaaggc tgcataggca ggccgccaga
     5881 catccatttc atggttcagg ccgggatatt cctggtaatc aaacttaatt tttttctgct
     5941 caagctcagt tttcagcccg gcgatatcct tgccggttac gacatcttta tcaccaacga
     6001 ccacagtaaa attacgtagt tgctggttaa tagctgccgg atcgttcagc cgggccgcga
     6061 caccttcatc cggtacggtt gtcgtggtaa caccactgaa tgtggccagc cagccaaagc
     6121 tttccagatg attcattccg gaaaccagcg cctggtaccc gccttgtgaa agccctgcca
     6181 gcgcgcgacc atcggcatct ttacggacac taaaacgctt gctaatcagc gggataatat
     6241 cgttcatcag ttcgcgatct gccgctttag cattcagcag ataaaagact ttacgtcttt
     6301 cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta tccgggatca
     6361 ccaccagcat cggtttaatt ttcccttcag ccagcaggtt atccatgatt tgcgggatac
     6421 gcccctgatc gatagcggaa cgtccagtat caccaaaacc gtgatagaaa tagagcactg
     6481 gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca gacatacatc tgacgttcag
     6541 attgcaatgc gttggagtgg taagttatgg cgatcaaatc accatgcgca acagaacgcg
     6601 tatccagata actgcctagc accagaatca tactggagtt aacctggcgc tgaggcttag
     6661 tcattgcggt gcctgtatca atgctgcgta caccatcaac attgaaaaaa tactcgtaca
     6721 ggttgccttt caggataggt gtgcgccacg accagactcc tgcttcgtct ttggtcatcg
     6781 ggtgaatatt gtccggaacc ggaacaccca ccactacaga gacatttttt gccccagggg
     6841 caaagtagcg aaaggtcacg ctgttatcgg cattgacctg agtcacatac tgtttcaccg
     6901 ggatagtggg tgccgggctg gctggcatat ccgctgctat ggcccactga gcagaaatac
     6961 cgctggcaat agccagcgtt aaagcagcaa tttttatatt catcgtaata ttcctttcat
     7021 taagttccat taccaccaga tttcagcctg ggcaccgaca gcaaactggt cgtctttatt
     7081 gtcttcgaag gtgaagccat ccagttcgtt ttccagggct ttgatatacg tggcgtagaa
     7141 acgaatttcc agacgcgagg tcaacatact ggtattgact ttaaaggtat ggaagagcgt
     7201 ggttttataa ccagactcat taaatttatt actattcgca tctttgtttt gctgggtgaa
     7261 atagcccagt tcaacacctg tctggttata ttggtcccaa atataggccg ggcgaacaac
     7321 cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg tagctataaa tatcgttacc
     7381 gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg ccgatatagg cttcgccctg
     7441 cgaggtcaga cgaactgctg ttccgccggt gtgatcacca taataacgac cattaaaggt
     7501 ggtaaatgga cttgcgccag cataacggcc aaagttcctg gcgatagagt tattcgcaac
     7561 caggaaggag aattcgttga agccaccttt atcaaatttc tgcgttaaag atgtgccgaa
     7621 catccaggta tctttccagt cataataccc gttattatcc tgattatctt tttccgatgc
     7681 gctttcgttt gccgtgacat aacgaccact caccattaag gtcgctttat cccataacgg
     7741 gatatcttta tagcgtaaat caatagtatg ggtattaatc tgctgtttgt tttgcaggct
     7801 gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata tcaattttac ccggaccgac
     7861 tttccagttt tccagaccta cacccgctgc ggcatcagta cgctgcgttt tccagtccag
     7921 catctggatt tcaattttcg gcgcaccgtg tttacccacc cagaaatcag cctctggcgc
     7981 aaagggcagg aaacctttgg tggtaacgta catatcggag aactgcataa agttctcgcc
     8041 accggcgtta tcgccaaacc agccggtaga gtactgctga ccaacgttac catccatcat
     8101 cacaacggca tcaacccgtt tgccgttttc gttgtagaca cgttgtttta actgcaaatc
     8161 aaaccagccg gagtattcgt taccaaagcg gcccagagaa ccaatcgccc atgatttagg
     8221 tgaaccatga gaggcggtcc cccaaccaga acggtaatag ccgttgtaac taaagccaat
     8281 ttcatctttc acaaacttac tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt
     8341 ctcactggca tttttctctt catttttgac cagtacagca tcaggttttg cggcactact
     8401 ggtcgggaac ggattggcgg catacccttg atcattcgta cttacgctac gattcaccgt
     8461 cgctggcgta tattttttct tctcttcatc tttatacttt ttgagttcgc tttgcgtttc
     8521 tcttaacgct ttttctaata actcaaggcg ttgttccacc gttaatgatt cagccaatga
     8581 ttgtgcagaa aaggctaacg gtgccattaa taagatggca gaggtaataa gatttcgtct
     8641 aaacataata tccctttatg gtgcaaagaa agaattaacg tatcgcatcc agactgttct
     8701 gaatgcgacg ataattaagg tgctttattg gttatttttt taatgacagc ccccgcgttt
     8761 tgatcacttc tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc
     8821 cattatcatc acgatccaca taaataaagc cgtagcgctt agacatttgt gaatgagatg
     8881 cactgactaa atcaattggc ccccaactgg tgtaccccat aatatccaca ccatcggcaa
     8941 tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc aattcgataa tcgtcctgta
     9001 tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc attctcgaca ataaataacg
     9061 gtttttgata acgatcccaa agcgtattta acagaacccg taatccaacc ggatcaattt
     9121 gccaccccct ctctgaactt ttcagatgcg gattggggat catattcagt atgttgccct
     9181 gcgcattttt attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag
     9241 agatgaaatc tacagtatgt tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga
     9301 tattgtggtc gcggaagaaa cgctgcatat agccgggata ctggccacgc gcctgaacat
     9361 caccaaagaa catccagcgc cggttctctt ccatggcctg caacatatcc tgtggctggc
     9421 aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc gattttcgct tcggggatca
     9481 ggctatgata ggctttaact gcccgcgcac tggcaaccag ttgatgatgg atcgcctgat
     9541 aaacttccgc ctcgccactc tcttctgcca gccccacgcc cgtgaatggc gcgtgtaacg
     9601 acatattgat ttcattaaac gtcagccata acgctacttt atgttggtag cgggtaaaga
     9661 ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg attagcccaa ccgccgtagt
     9721 ttttcaccag cccatatagc atttcgtaat gggataacgt taccagcggc ttgatccccg
     9781 cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca ttcggttcgg
     9841 cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc
     9901 ccatctcggc aaataacgcg atatcttccg ggtaacggtg ataaaaatcg atggcgacat
     9961 ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccattacg ccatgaggct
    10021 gtaaatctga ggtcgtgatc cctttgccat cttccagcca ggcaccttcc acctgattgg
    10081 cagctgttgc gccaccccaa agaaatgttt ctggaaatgc tttcgtaatt aactcctttt
    10141 atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg
    10201 gtaatacgtc tgtaaaatca tcgctattac tgattaatac cggcgtcgtc agatcaaatc
    10261 cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt
    10321 cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga
    10381 catgaatcag gatctccaca ccatcatctg actcaatgcc aatggcgtgt aatgtggcga
    10441 acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg
    10501 cgataccttt acccagcagg ccactggcaa acgtggtatc agcgacgtga atgagcggca
    10561 caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg
    10621 gtgttttctc ttgcggggca ccctgcgctg gctgacgttt actggtgatg aaatgaagca
    10681 tcaacgtacc aacaaatgcg caacctatgg caatgacacc gccaataacg ctggcccaga
    10741 cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa ggtgaaaata cttggcaaac
    10801 caaaggagta aactttcgtt tgcgcgtagc caatgatggt ggcccccaat gctccactga
    10861 tacaggcgat aacaaagggg tacttacgcg gcaggttgac gccatatacc gctggttcgg
    10921 tgataccaaa cagacccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc
    10981 gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg ggcattaaca
    11041 gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc
    11101 cccagtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc gcgcccgcaa
    11161 atgcaggaac cgcctgataa agccagagat aaccggcggc aatcatttcg cttatccagg
    11221 ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga catagcaatg
    11281 gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc
    11341 tgcacaacca ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga
    11401 ccggaatacc caggaaatcc agccccggca catccgcttt ttgcccgttc tcgaaggcag
    11461 tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc
    11521 cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa gaacccaaac aaggcatcac
    11581 tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg
    11641 ccagagccag catacctttc aggatcccgg ttgccgccat cagaccgatc agaggcgtaa
    11701 aaatacctga aataacataa acaaagcggt ttagcagatt acctttctca tcattttccg
    11761 gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt aaccgccagg aagacatcgg
    11821 ccacatggtt acctatgacc acctgaaact gaccaccgct ttccaccacc ataataatac
    11881 cgggggtctt tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta
    11941 atcgcgttgc acaatgcatc agactcacaa tgttatctgc gcccccgact cctgcgacta
    12001 tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa
    12061 aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt
    12121 agtgcggtaa caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct
    12181 atatttatgg ctaaaaacat aatctctgcg ggtgaaattt tacgttgata ctgcaaacca
    12241 ataaaaatgg cgatccgttc cgcacattgc catgcttgcg ggtaattttg ttttactgct
    12301 tgttgtaatg attcatcact atcgttaatt gaagcatgtt caagaatacg ccaggataaa
    12361 aacttcagat gtgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg
    12421 aactgaaatt ttattaattg cagcatttcg cgcattaact gcgtgacacc tgcaacatcc
    12481 tccatatttc cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct
    12541 ttcggtaact gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg
    12601 aactctttcg ggtaaagccg ctggatatcc cacagcaacg ggttgggcag caacacgttt
    12661 tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc
    12721 tgtaattttc ccaagcgctc ctgcgctaaa gagataagac gatcacaggt tgccatcacc
    12781 tcaagaggaa tatgacttaa gagttcgctt aatcg
//
LOCUS       DAJTYI010000111        12736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYI010000111 DAJTYI010000000
VERSION     DAJTYI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(8..355)
                     /locus_tag="OO564_003049"
     CDS             complement(8..355)
                     /locus_tag="OO564_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6654088.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(446..790)
                     /locus_tag="OO564_003050"
     CDS             complement(446..790)
                     /locus_tag="OO564_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654089.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVDLLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(817..984)
                     /locus_tag="OO564_003051"
     CDS             complement(817..984)
                     /locus_tag="OO564_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654090.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(1172..2011)
                     /locus_tag="OO564_003052"
     CDS             complement(1172..2011)
                     /locus_tag="OO564_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6654091.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(2013..2699)
                     /locus_tag="OO564_003053"
     CDS             complement(2013..2699)
                     /locus_tag="OO564_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6654092.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(2686..3000)
                     /locus_tag="OO564_003054"
     CDS             complement(2686..3000)
                     /locus_tag="OO564_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654093.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2997..3482)
                     /locus_tag="OO564_003055"
     CDS             complement(2997..3482)
                     /locus_tag="OO564_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654094.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(3479..3643)
                     /locus_tag="OO564_003056"
     CDS             complement(3479..3643)
                     /locus_tag="OO564_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6654095.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(3654..3947)
                     /locus_tag="OO564_003057"
     CDS             complement(3654..3947)
                     /locus_tag="OO564_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR6654096.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3961..4467)
                     /gene="ssb"
                     /locus_tag="OO564_003058"
     CDS             complement(3961..4467)
                     /gene="ssb"
                     /locus_tag="OO564_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6654097.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(4468..5175)
                     /locus_tag="OO564_003059"
     CDS             complement(4468..5175)
                     /locus_tag="OO564_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6654098.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(5430..5582)
                     /gene="kil"
                     /locus_tag="OO564_003060"
     CDS             complement(5430..5582)
                     /gene="kil"
                     /locus_tag="OO564_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR6654099.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(5567..5698)
                     /locus_tag="OO564_003061"
     CDS             complement(5567..5698)
                     /locus_tag="OO564_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR6654100.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5723..6691)
                     /locus_tag="OO564_003062"
     CDS             complement(5723..6691)
                     /locus_tag="OO564_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR6654101.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6859..7101)
                     /locus_tag="OO564_003063"
     CDS             complement(6859..7101)
                     /locus_tag="OO564_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654102.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(7304..7636)
                     /locus_tag="OO564_003064"
     CDS             complement(7304..7636)
                     /locus_tag="OO564_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6654103.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(8097..8501)
                     /locus_tag="OO564_003065"
     CDS             complement(8097..8501)
                     /locus_tag="OO564_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654104.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(8498..9130)
                     /locus_tag="OO564_003066"
     CDS             complement(8498..9130)
                     /locus_tag="OO564_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6654105.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            9234..9449
                     /locus_tag="OO564_003067"
     CDS             9234..9449
                     /locus_tag="OO564_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6654106.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            9569..9862
                     /locus_tag="OO564_003068"
     CDS             9569..9862
                     /locus_tag="OO564_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCR6654107.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9895..10041
                     /locus_tag="OO564_003069"
     CDS             9895..10041
                     /locus_tag="OO564_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCR6654108.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            10034..10867
                     /locus_tag="OO564_003070"
     CDS             10034..10867
                     /locus_tag="OO564_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCR6654109.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10857..12293
                     /locus_tag="OO564_003071"
     CDS             10857..12293
                     /locus_tag="OO564_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6654110.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            12370..>12736
                     /locus_tag="OO564_003072"
     CDS             12370..>12736
                     /locus_tag="OO564_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6654111.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTE"
BASE COUNT         3055 a         3131 c         2830 g         3720 t
ORIGIN      
        1 tctgctttca gacgatcgtg aagttcttgc gcttgccttt tgtccgatgt ttcaagagag
       61 cgtctaactc ttgatccatc tggcgcgacg aaatcgcagt gccacgtgcc accgcgtagt
      121 ttgattgaca tgctttaacc tcctgcacat caaccgcatt caccgcgcta ttgtgtctca
      181 cagacttaag cgccgcaatg cagtctgact tgcaaatgcg atatgggctt ttaggtttat
      241 ctggatttat ctttacggcc tgaagtcgtc cacttcgtat ccactgcgtg atagtgcctt
      301 tgtctacctt cagatacgac gctgcctctt cacgagtgaa gatttcttct tccacctgga
      361 atctccattt attggattgg tattattgcg gtaggtctgg atatcattga gcaatgaaca
      421 ggcctcatcg agtatgaggc tgtggttagt ccttgcgtaa ctcgctaatt cttctgtaag
      481 tctctggtgc tttgtttccg tgtatcttca tttcagactt caacagatca acgagggaat
      541 cccattcgtt gaggatgcct ttgaatgccg gaacgcgctt tgcaaccttg tcgaatgaat
      601 ctctgatttc tgggatctgc tcaacaagtg caacgcatcg tctgaaatcg gctgcgtcat
      661 ggggagcgcc gaagtgatga ccatagatat tctttttcag tccacatgcg attgaggcaa
      721 gagttgcgct actgatgcca acatcgccag tcgattgcca tttcaaaacc ttcatagcca
      781 aatctgacat ttcttgtctc caataaaaaa ccgccatcag gcggcatggt gttctttcag
      841 ttcctcaatt cgaatattgg ttacgtctgc atgtgctatc tgcgcccata tcatccagtg
      901 gttatagcag tcgttgatgt cctctgcttc gataaccctg tcgaatggct ctccattcca
      961 ttcacctgtg actcggaagt gcatttatca tctccataaa acaaaacccg ccgtagcgag
     1021 ttcagataaa agaaatcccc gcgagtgcga ggattgttag ttgctatctg ctgcttcctt
     1081 ggtcattacc atatccaccc aatttcacca gacatgattc tcgcaatcac tattatcacc
     1141 aaagtgataa tcacaacttt aactggcggc atcattcacc atcctgctcc ggcggttcta
     1201 gcagcggcat ccagtgggtt acgtcatcca agatatttcc tgataaatat gtgaaagctc
     1261 tatatttttt gtaatcaatt ggactcatta cccagttcca atatgctgcc actatttgac
     1321 cttgactaaa tgccagtaac attttggtgt cttccggcat tcgctcacta cagcttatcc
     1381 aaccatccgg agttaccgga tagttgcgca ttgcgacctt taatgcctca tagaagcaac
     1441 ctttcagatt gttgaactga cgcccattta gcgggccatg ttcagtcagc atgttgtgta
     1501 atttccatgc cgcgtcgttt acttcgttgg atgacagggg aggcaacttg taagtttggc
     1561 ttacaggttc ggcaccatga agcatggcgt cgctccgctc tatgccatcc agcgcgattc
     1621 gtagtgcctg aattgtggta gagctatcgt ttggggctat tccatatcgc tcgaatacag
     1681 ctaaatggtt gcgcataatc tcaggcgtaa gctctttgta agcataagcg agaggctctg
     1741 atgcattatc cggcacaacc gacgcaggcg cggcagcata aacaggaata acgtccgctt
     1801 gctctttatt gctttcatcc gttaaagccc agaataattt cccggccgga tgtttgaaaa
     1861 tataagcaac tggctctgct tccagcgatg ccagtgcaat tcgtgccagt tcttccgctt
     1921 cttctgctgg cagtacaacg ttgctaccag gtccgtatgt ttcgcgccac tgcttgattg
     1981 tcagtagtcg ctctttggta atagtggtca tctcactctc ctttgatgcg aataccagta
     2041 gcgcggattg catcgatgac ttcagaaact ttgtatgcca ttaccgtttg gtaatcctcg
     2101 tgaaaatctg ttcgatgaag catgctgcta cgttccggga gcagtatttc ccgctcctcc
     2161 agttctgcta tgcgcttctc tgcggattcc agcttctcgc gcatatcgtc aacgtactcg
     2221 accagagaac cgccagcagg aatttcgcac tcctcgacca gttggaagta gatatcagct
     2281 gcggcccgtg tgttgctata cctagcgtcg cccatctcac cttcacgaag agcatcgcgt
     2341 tcggcggtaa gattggctat tttgctgtct ttgccttcca gctcaacgcg cagcttccct
     2401 accgtaagcg caatatcctc gttctcctgg tcgcggcgtt tgatgtattg ctggttcctt
     2461 tcccgttcat ccagtagtgc ctgcgctact tcagggttga aagctgcgat ataacgagcg
     2521 ttgttctctg cgttgttctg tccatcaaag ccggtccatt tgataacgtc ttcacatcgt
     2581 ttatcaccag gtgtatgcac cgcatatgta ccagtaccag acgaaataaa tgcgacccat
     2641 tcaccctgtg ttgccagttg cgccgcttta cgcagtgcct ggtaattaat ttcgctcact
     2701 ggttgcctcc tttgctgggc tttctaactt ttgagtggtt gtatcaaatt caaacaactt
     2761 aaccacgtca tcaaacagga cataatcacc atcagaatct tcagtcatgt cagcgccaca
     2821 atcctgaccg catgagtcac aaccacccat atcaagctcg tatcgcttca ggtttgcgat
     2881 atttgataaa ttcagcgcca gtacagccag gtcataaacc tcttcggcag tgacatcgct
     2941 gctcagtccc atttcatggc gatatatgat tttttctact cgttgttttg tgattgtcat
     3001 ttttgctcgc ctccctgttc ttccagaaaa atacgcatag tctcaagcat ctcttcggtg
     3061 tcatacggtg acaacttgtc acgcaggatg tgttcaatgc tgttaatgaa cttgcggatt
     3121 gctttgcgtt caatttcagc cagaaaagca tcggttgccg gggtttcagg tttaatggct
     3181 tcaagcacgg cgcgaatgac atcggtatcg ttctccaccc atgaccattc gcttgtatca
     3241 ttccagtcgt ggtctatgat agctgtttca ttgaatgcct cgactgccgt atccgggatt
     3301 acttcatgat ttaaagctgc cttcagcccc gcattctccg ccgccagcgc cgcgcacttg
     3361 gcctccgctt cagcaaattt acgcaccaga tattcagcgt ttgtttcgtt aacctttaaa
     3421 tcacttggga tgcatttacc tttcagaaat ccatccatct caattagtgt catttgtttc
     3481 atttcttccc attccgccac attgcattca gatatttgtt ttgatttact gacggaaaag
     3541 aatttctttt aagcaattcc tctctcgatg gcattggctt tacgcgttgg cgaataatca
     3601 tttctgccgg aagaatgccg agattgtatg caagtcctct catggtaaat tcctcagtca
     3661 ttactgatag cgccatagcg tgagcggtaa ttacgcaggc gcgggtcaat ttcagggaag
     3721 tgggtatatg tggctttgcg gaatggtcgg attgatgtct ggtaaattcg ctcgcgctct
     3781 tctttctctg caagccatat gcagtggcga aattcctttt cctctttcgt ttcctgcggt
     3841 agcgacatta tccggtcgta gtttttcctg aatttatcca gcacctccga tacggaattg
     3901 ccggaacagc ggcgcgggtc atccgcacca tactgaggcg ctggcatgat tttctcctga
     3961 ttaaattgcg tgaatagcgt gacgagggaa ggggagagtt actggtgcaa agggtatatc
     4021 gtcgtcaaaa tccatcggag gttcgttgtg ttgtgctggt gatgattgct gctgtggctt
     4081 ctgtgattgc ctgctggctg cttgttgttt gctgtcgcca atgccgccaa gcatttgcat
     4141 cacgccatta attccgacat gaacctcggt tgtgtaacgg tcttgccctg actggtcttt
     4201 ccactttctg gttctcagca ttccctcgaa ataaatctga tcaccttttt tcacatactg
     4261 ccccacgacc tcagccagtt tcccggatac agcaacacga tgccattcag tcaattcctt
     4321 ttgctcgcca gtatttttat ctcgccattg ttctgacgtg gctattgtca ggttagcgaa
     4381 cgctgttcct gatggtgagt atcgaacttc cgggtcttgt cctacccgac caaggataat
     4441 caccttattt acgcctctgc ttgccattta tgccgcctgt tttagttcgt taactctgat
     4501 gttcattacc tgaacgcatt tagcctgcgc ttcctcgttg ccagccatta attgccagtc
     4561 acgctgataa cgctcgatga gttttttctt gtcagtttct gttgacgcat aatcgctgaa
     4621 gtctttcagg atttgttcgc agtcaaccga tggagatttc tggttggtat tttctggtga
     4681 tggtttgtta tctgatgctg ggattgccca tcccggcagc gatggaggga gccagtaaaa
     4741 tcctgttcca tccttcagtt ttgccctgtg ccacccatgc tttttatcga gagatgtttg
     4801 tgcgaaacct tcctcaaggt tatacagata ccgaccgatt ccccactgaa cggcagcacg
     4861 cttcattgca cctgaacgac cacctttgac ggcttctacc tgcgtgtttt cagcagcatc
     4921 ccatttggtt acccattcgg aatcaatcct gattgatatg ccgcattcaa cgcctccgtt
     4981 gttgggaata tcgcggtatt cattgcgcca tcctgctttg ccgcaaacat cgtccaggcg
     5041 tttcatgatt gcccggttcg tgacataagc cagcaccata gcccacactt tgccatcgcg
     5101 tgttttaccg ctttgctgta ttcgccattc gatatcttca gggctgaatg gctcatcgaa
     5161 tttattcaaa tccataaatc acctcagaat ggacatggcc caagaaaata acgctgattt
     5221 aatacttcga ctcgggacaa attaaggcat acccgcattc cttcgcggtc gccattatgg
     5281 cgataccaga gagctttctg ggtgtacatg cgtctctgta acttgctctc cttcactgtg
     5341 gttgcaagtg acatgaatat ctccttcgtt tccgattaat tctttcatct gacgaatgaa
     5401 ttcttcgtct gaccagttat ctgtaaaact catttcctgc gataccacgg aaggttgata
     5461 gctgacttca tcgctttatt tgcttcaagc cacattttgg aatcaccaat aaatctggct
     5521 attactgctt tgttctgtgc agcacgaagc atctggtgat tgatggctat ttcattgcgc
     5581 ataataagac atcaactctt ttccatccgt cacgtaattt acgggtgatt cgttcaagta
     5641 aagattcatt taattggaag gcacccatgc gagcgcctcc cgcgattgcg taaatcatgg
     5701 gtggttcctt atgttggttt tattagtagg ttatttttgt tgcgaatact tcgcctttta
     5761 cgatggctgt tatgatattt ttagcaacat cttctgatgc gccaaccttg ataaggtcag
     5821 caagtatttt gttatttact tcttttcggt gagctttatc ctttgctcta cgctcttctt
     5881 cgtccttgat tcttttttct tctgctattc tggcttgctc ttttgcttca gcttcgcgcc
     5941 ggattcgttc agcctcctcc tgtgcttttc ggcgttctgc ttcaattgcc gcctgctttt
     6001 ctctttcagc tcgttctgct gcctcttttg cttcgcgctg tgctcgttgc tcggcttcaa
     6061 tgcgttcacg ctttgcacgt tccgctgcgg ccttagcttc tgcttctcgc cttgctgctg
     6121 cttcaatttc ggcttttgcc tttgcttcgg cttctgctct ggctttctct tcagcttctc
     6181 tttttaagcg ttcttcatgc tctcgctttt cctgctccgc tttgagtctt gcctcttctc
     6241 tttggcggtc aaattcgcga tccatcaaaa tcgctatttc atggtcagac tcaatttgct
     6301 ttgcgagagc ttcagctgct gccttagctt cttcttcggc tttaatccgc gcctgttcct
     6361 cctcataatc agtaagaggc tggcgcgcct tggctttcag ctcatcaagt cgatcatgca
     6421 ctgttttgcg gttggcatca attagctttg gaatttcctt cagttcagca acaaggtctt
     6481 tgccaagacc atcgagatat gttttcgtct gcgcaacttt atacgccaga gaagcgatct
     6541 cctttctgcc ctttgccgtt gtgatatcag gcacaaagga cataacttca cgttcaacct
     6601 tttgaaggat ttcttcaatc tggtcggcag actgaaatac agtcattgca tttgcttttt
     6661 caataacaac taaatctgtt acttcactca tatttccccc gtcaaaaaaa ttgccctcac
     6721 actggagggc aaagaagatt tccaataatc agaacaagtc ggctcctgtt tagttacgag
     6781 cgacattgct ccgtgtattc actcgttgga atgaatacac agtgcagtgt ttattctgtt
     6841 gcttattcca aagcaaattt aaccatcaac aaactcttct ggtaatttat caaccagttg
     6901 atgacttatt atcagccatt tgccatcctt cgtttcgtat gcatatttct ggtcttttat
     6961 catcatgtgt tcggctactg ccctcactgc ctgatcggtg acatcttctc tctttcctac
     7021 ccacattcct ttttcagtgt ttaatgttcc ttgaaaaata cgaccactta ataggcttgc
     7081 gcccatagtt tttactctca tgtatcagtc ctcaaataag tggtttgctg cgaaagtaaa
     7141 tacgcttaag ttacctgtta tttatcccac caagttccgt atctatctat ccagttacac
     7201 caatcatcga cactctattt tgttgtgtcg cattttggca actggcaaga atatctacct
     7261 tctttgtaaa gtcggcgttt gactttcttg agcatggctc acctcaatcg taataagctg
     7321 gaattgattt tccgcgttgc ttctggcggc ctgagcaagt cacacctatt tcactgcgtg
     7381 gcttgctgta ccatgtgcgc ttattcttgc gctcagtacg ctgcaggttg ctttcaatct
     7441 gttcgtggta ttcagccagc accgtaaggt ctatcggatt cagtgcgctt tctactcgtg
     7501 atttcggttt gcgattcagc gagagaatag ggcggttaac tggttttgcg cttaccccaa
     7561 ctaacagggg atttgctgct ttccattgag cctgtttctc tgcgcgacgt tcgcggcggc
     7621 gtgtttgtgc atccatctgg attctcctgt cagttagctt tggtggtgtg gtggctggta
     7681 gtctagctcc agcttgttga gtctcattcg gaggggtata accggcaccc cagcgatttt
     7741 tccatgcgac aacgtgcgcg ttatggcggc cttatcgccc gcggctcccc atctcgtcca
     7801 cgctattgct agcgttggga gcgcttcacc gctcaacagt aggtaagcac ttgccagtga
     7861 ctagctggct tcaccacacc ccaaagcctt ctgctttgaa tgctgccctt cttcagggct
     7921 tattttttaa gagcctcacc ttcatggtgg tcagtgcgtc ctgctgatgg cttaaaatta
     7981 caagaaagat tgtatgttgt aaacaagaaa tattgtgaaa aggggcgtga aaaacaaact
     8041 ccattgtttt taaatgaaaa atagtttgtt tttggttatc gagattgagg tggggattac
     8101 tggttgcagg ttccgactac atcaccaaca aatgatttgg ttgatgtaag ttgttgcata
     8161 cctgggatat tcattacttt ggagtaaaga gcttttttgt ctgtagtgat tgaccaagtt
     8221 tcaacggtta ttcctcctcc agactggtat tctcctacca tagtgttcga tgacaaagca
     8281 gtgtatttca tctctggata gacgccagaa actgattcat aaactgatga tttatcgcca
     8341 tttattgtta cgtggaaaac ggaatcttcc gtgctgtctt ttgtaaactc gtaacgatcg
     8401 ccattcattg acccgtaccc gtgcaggttt gtgacaatcc agcattcaga attggcgctg
     8461 gtagttaaga gtattgagag tagcgccgca atcctgatca tacgaatttt accctcgctt
     8521 ccacgacaac accgataatc ttgcagttcc cgttgatagg agtcataggc catgaagggt
     8581 tcaggccttt caggtacttc tgcccgccat ctataaccag tttcttgaat gttgcttcgt
     8641 tcgcgtcagt cagtttggct acaacaaggc ttccattcac tggctcgcgt ccagtatcta
     8701 ctaacaccat atgaccttca gggatgcttt gacctacagg tgaggtcatg gaatcacctt
     8761 caaccttcag ccagaatcca tcgcctaata agttaacgtc actgtcatac cattcatcaa
     8821 tgtccttgat atcgtagggt tcacaagctt cacaccacga accagctcta accatgctaa
     8881 tcaatggata tttccctttg ggttcaacat gcccaacaaa tctaacattc gaatcagagg
     8941 tgccattgag cagccagtca acacttacgc caagagctga cgcaagttct ggtaaaaagc
     9001 gtggtcgctt agttttaccg ttttcgagct gctctataga ctgctgggta gtccccacct
     9061 tttgagcaag ttcagcctgg ttaagtccaa gctgaattct tttgcttttt accctggaag
     9121 aaatactcat aagccacctc tgttatttac ctccaatctt cacaagaaaa actgtatttt
     9181 acaaacaaga tacattgtat gaaaatacaa gaaagtttgt tgatggaggc gatatgcaaa
     9241 ctctttctga acgcctcaag aagaggcgaa ttgcgttaaa aatgacgcaa accgaactgg
     9301 caaccaaagc cggtgttaaa cagcaatcaa ttcaactgat tgaagctgga gtaaccaagc
     9361 gaccgcgctt cttgtttgag attgctatgg cgcttaactg tgatccggtt tggttacagt
     9421 acggaactaa acgcggaaaa gccgcttaag acattcccgc tcttacacat cccagtcctg
     9481 aaaaagggca tcaaattaaa ccacacctat ggtgtatgca tttatttgca taccttcaat
     9541 caattgttat ctaaggaaat acttacatat ggttcgtgca aacaaacgca acgaggctct
     9601 acgaatcgag agtgcgttgc ttaacaaaat cgcaatgctt ggaactgaga agacagcgga
     9661 agctgtggga gttgataagt cgcagatcag caggtggaag agggactgga ttccaaagtt
     9721 ctcaatgctg cttgctgttc ttgaatgggg tgtcgttgac gacgacatgg ctcgattggc
     9781 acgacaagtt gcttcgattc tcaccaataa aaaacgcccg gctgcaaccg agcgttctga
     9841 acaaatccag atggaattct gaggtcatta ctggatcaat ccacaggagt aattatgaca
     9901 aaacaactca gtccttacca ggacaaaatt cacaaacaca tactacgtga tcgcttcctg
     9961 tccagcttca agcagcctgg tcgattccgg gctgagttgg aaaaagtgaa gctgatgcag
    10021 aaggagaaag gtcatgagta acatatctaa tctagccgaa gccagagagg ccagaaggct
    10081 acaacaaccg catcaaagca gcggtaaggg gtatgccttg ctgcaccgta aaattatgga
    10141 tgtgccgttt tacaaggacg cagaagcagc gcatctgtgg gttcacttaa tcctcaaagc
    10201 aaagcatacg cctgagtatg taatgactga cgcaggagaa attctggtag gcagagggaa
    10261 gctacttggc ggtagaaact ctctggcgtt tgaaacagga ctcaaaccag atcgcgttca
    10321 gtacctgctt agaaagttca aaaaactcgg catgattgac tgggtttcac acggtaaatt
    10381 ctcagttttc tcggtagaga aatatgacga ttatcagtca aattttgtac cagcagatta
    10441 ccagcaaatt accacagata aagaatataa taatattatc tctaatactg acgtattaga
    10501 gagtaccgca gcagacaaaa agtctgacaa gaaaaaacct tccgttagct gtcaggatgt
    10561 tgtcgatgct taccacgaaa tccttcctga agcgccaaaa atccgcgcac tgaatgacaa
    10621 gcgtaaaaac cagatccgaa cgttctggcg caaagccgga gtgataaccc gccagcttga
    10681 cgggcatggg ttcacgatgc aggactggag aaattatttg agctacgtag gcgaaaattg
    10741 ccgatggatg ttcgaagagc gcccaaacca tcagcgcgga accgtctggc acaaaaaggg
    10801 atttgatttc ctgcttaacg ataataccta cctgaaagtt cgtgagggtg aacacgatga
    10861 ccgataattt ttatgcgccg ccccatagca tcgaggcaga gcaggcggtg attggtggat
    10921 tgcttctgga tgatgacagc agtgagcgcg tccagaaggt tctggcgatg ctgaagcctg
    10981 attcatttta cagccgacca cacaaaatca ttttcgaaga aataaccaga atgcaccggg
    11041 agcaaaagcc agtagatggc ctgacgcttt tcgatgaact ggagcgtaaa tcgttaacgg
    11101 cgtctgttgg cggttttgct tatatcgctg agatcgcaaa gaacacgcca agcgccgcaa
    11161 acatcgttgc ctatgcaatg caggttcgcg aaaccgcaat ggaacgctac gccatcaacc
    11221 gcatgactga agcgacggaa ttgctctatt cccgcaacgg aatgactgcg acgcagaagt
    11281 acgaagctat tcaggcgatt ttcacgcaac tgacagacca tgcaaaaaca ggatcgcgtc
    11341 gcggccttcg ctcatttggt gaggtcatgg aagactgggt tagcgacctt gagaagcgat
    11401 ttgacccatc aggcgaacaa cggggaatga gcacagggat cccatcgctg gacaggatgc
    11461 tgtcaccgaa aggtctggtg aaaggctctc tgtttgtcat tggcgctcga cctaagatgg
    11521 ggaaaacgac gctatacagc cagatggcaa tcaactgcgc agtgcatgag aaaaagcccg
    11581 ctcttatgtt cagccttgaa atgccaggtg accagatact ggaaaaactg gtagggcaga
    11641 agtctggtgt taacccgaat attttttacc ttccggcgac aaatgacgct gatgacggct
    11701 atcagggtga ttacgatggt gacttcaaca gggcgatcga aacagccaat cgcttgagtg
    11761 aaatcgacct gctttacatc gacgacacgc cgggattatc tctggctcaa atcgtcagcg
    11821 aaagccgtcg aatcaagcga gaaaaaggat gtgttggcat gattctggtc gattacctga
    11881 cactaatgac cgctgaaaag gccgatcgca acgaccttgc ttacggcatg atcaccaaag
    11941 gactgaagaa ccttgccaaa gagcttgatt gcgttgttgt gcttctgaca cagcttaacc
    12001 gcgcactgga aagccgaacc aataaacgcc cattaccaag tgactcacga gatacagggc
    12061 agattgaaca ggattgcgat tattgggtgg ggatccatcg tgaaggtgct tttgatgaca
    12121 gtgttccacc tagtgaaacc gaactaatcc ttcgtctcaa tcgtcatggc aataccggca
    12181 cggtgtattg cattcaggca aatggcgcta tttatgacac agaccaacag tctgctgaaa
    12241 tgcgccgacg tgaacgcgag gaaccgcagt ccaagaagaa aggaggattc tgatgaccat
    12301 ctacatcact gagctaataa caggtctgct ggtaatcgca ggccttttta tttgggggag
    12361 agggaagtca tgaaaaagct aacctttgaa attcgatctc cagcacatca gcaaaacgct
    12421 attcacgcag tacagcaaat ccttccagac ccaaccaaac caatcgtagt aaccattcag
    12481 gaacgcaacc gcagcttaga ccagaatcga aagctttggg cttgccttgg tgacgtctct
    12541 cgtcaggttg aatggcatgg tcgctggctg gatgcagaaa gctggaagtg tgtgtttacc
    12601 gcagccttaa agcagcagga cgttgttcct aaccttgccg ggaatggctt tgtggtaata
    12661 ggccagtcaa ccagcaggat gcgagtaagc gaatttgcgg agctattaga gcttatacag
    12721 gcattcggta cagaac
//
LOCUS       DAJTYI010000112        12589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYI010000112 DAJTYI010000000
VERSION     DAJTYI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..631)
                     /gene="rutR"
                     /locus_tag="OO564_003073"
     CDS             complement(<1..631)
                     /gene="rutR"
                     /locus_tag="OO564_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6654112.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIR"
     gene            919..2010
                     /gene="rutA"
                     /locus_tag="OO564_003074"
     CDS             919..2010
                     /gene="rutA"
                     /locus_tag="OO564_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6654113.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2010..2702
                     /gene="rutB"
                     /locus_tag="OO564_003075"
     CDS             2010..2702
                     /gene="rutB"
                     /locus_tag="OO564_003075"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6654114.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2714..3100
                     /gene="rutC"
                     /locus_tag="OO564_003076"
     CDS             2714..3100
                     /gene="rutC"
                     /locus_tag="OO564_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6654115.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3108..3908
                     /gene="rutD"
                     /locus_tag="OO564_003077"
     CDS             3108..3908
                     /gene="rutD"
                     /locus_tag="OO564_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6654116.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3918..4508
                     /locus_tag="OO564_003078"
     CDS             3918..4508
                     /locus_tag="OO564_003078"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6654117.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4519..5013
                     /gene="rutF"
                     /locus_tag="OO564_003079"
     CDS             4519..5013
                     /gene="rutF"
                     /locus_tag="OO564_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6654118.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5034..6362
                     /gene="rutG"
                     /locus_tag="OO564_003080"
     CDS             5034..6362
                     /gene="rutG"
                     /locus_tag="OO564_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6654119.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6444..6551)
                     /locus_tag="OO564_003081"
     CDS             complement(6444..6551)
                     /locus_tag="OO564_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654120.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6924..7520
                     /gene="wrbA"
                     /locus_tag="OO564_003082"
     CDS             6924..7520
                     /gene="wrbA"
                     /locus_tag="OO564_003082"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6654121.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7541..7768
                     /locus_tag="OO564_003083"
     CDS             7541..7768
                     /locus_tag="OO564_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6654122.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7806..9047)
                     /gene="agp"
                     /locus_tag="OO564_003084"
     CDS             complement(7806..9047)
                     /gene="agp"
                     /locus_tag="OO564_003084"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6654123.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9340..10596)
                     /locus_tag="OO564_003085"
     CDS             complement(9340..10596)
                     /locus_tag="OO564_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6654124.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10857..11777
                     /gene="cbpA"
                     /locus_tag="OO564_003086"
     CDS             10857..11777
                     /gene="cbpA"
                     /locus_tag="OO564_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6654125.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11777..12082
                     /gene="cbpM"
                     /locus_tag="OO564_003087"
     CDS             11777..12082
                     /gene="cbpM"
                     /locus_tag="OO564_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6654126.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12175..>12589)
                     /gene="torD"
                     /locus_tag="OO564_003088"
     CDS             complement(12175..>12589)
                     /gene="torD"
                     /locus_tag="OO564_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6654127.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKILTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3010 a         3211 c         3167 g         3201 t
ORIGIN      
        1 gtcgaatccc ctcaataata atccgctgca cgttttcaac cgtttgattg aaaaatacct
       61 catcgcgcaa cgtcgcgcct gtcaccgcct ccacctgagg ggcgaaatcg gcgtaatgtt
      121 gagtggaagc ccaaatcata aaaatcaaat gctgcggatc aatcggcgcg agtttgccgc
      181 ttttgaccca accggcaatc agcgccgatt tctcatcaat taatgccttc aaatcgcccg
      241 tcagttcatc cattaacaac ggcgcgcctg ccagcatctc catacagaac aggcgcgaag
      301 cctgcggata atcgcgtgag acttccagct tcagacggat gtactctttt atcgccgcca
      361 gcggggcgaa atcttcacga aacgctttta acggtgccag ccagatatcg agaatctgcc
      421 gcagcacggc aatatacagc gcctcttttg acgggaagta atacagcaga ttggtttttg
      481 aaacacccgc caactctgcg atctgctcca gccttgtgcc gtgaaaaccg aattgtgaaa
      541 aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc gcttactgcg cgcgaacgtt
      601 tacccgttgt tttcactgcg ccttgcgtca tgcgctctcc cctcttgtag attggtaaca
      661 ggatagcaaa acccccgccc ggcctcgacc ttctgcactc tcatcgcgca cggatgcatg
      721 ttttatgtgc aactgttttg accgtttagt ccacttttta ccagatattt tagataacac
      781 aatgctaaca cattaataac attcactttt taaaaactgg catccgcttt gcaaacaagc
      841 caatacgcgg ctgaaaagga tatgcaggat gcagcgcccc gtctgacttt cactttacga
      901 gacgaagaga ggttgatgat gaaaattggc gtattcgtac ctattggcaa caacggctgg
      961 ctcatttcga cccacgcgcc gcagtacatg ccgacctttg aactgaataa agccattgtg
     1021 caaaaagcgg agcattacca tttcgatttc gccctgtcga tgatcaaact gcgtggcttt
     1081 ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt tcaccttgat ggcgggtctg
     1141 gcagccgtga cctcgcgcat tcagatttac gccactgctg ccaccttaac gttacctccg
     1201 gcaatcgtcg cccgtatggc cgcaaccatc gactccatct ctggcgggcg ttttggcgtc
     1261 aacctcgtga ctggctggca aaagcccgag tatgagcaga tgggtatctg gcctggcgat
     1321 gactatttct cccgtcgtta cgactatctc accgaatatg ttcaggtgct gcgcgacctg
     1381 tggggcacgg ggaaaagcga ttttaaaggc gattttttca ccatgaatga ttgtcgcgtc
     1441 agtccgcaac cgagtatccc catgaaagtg atctgcgccg ggcaaagcga cgctggcatg
     1501 gcgttctccg cccagtatgc cgatttcaac ttctgtttcg gcaaaggcgt aaatacaccc
     1561 acggctttcg ccccgaccgc tgcgcggatg aaacaggccg cagagcaaac cggacgcgac
     1621 gttggctctt atgtgttgtt tatggtgatt gccgatgaaa ccgacgatgc cactcgcgcc
     1681 aaatgggaac actacaaagc gggcgcggat gaagaggcgt taagctggct aaccgaacaa
     1741 agtcagaaag atacccgctc cggtactgac accaacgttc gtcagatggc cgatcccact
     1801 tcggcggtaa acatcaatat ggggacgtta gtcggttctt acgccagtgt cgcgcgcatg
     1861 ttagatgaag tcgcaagcgt gcctggtgca gaaggcgtgc tgttgacctt cgatgatttt
     1921 ctgtcgggaa tcgaaacctt cggcgagcgc attcaaccac tgatgcagtg ccgcgcccat
     1981 ctctctgcgc tgactcagga ggtggcatga tgacaacctt aaccgctcga ccggaagcca
     2041 ttaccttcga tccgcagcaa agtgcgcaga tcgtggtgga tatgcaaaac gcttatgcca
     2101 cgccaggcga ctacttagat ctcgccgggt ttgatgtctc aaccactcgc ccggtcattg
     2161 ccaacattca aaccgctgtg accgcagcgc gagcggcagg gatgttgatc atctggtttc
     2221 aaaatggctg ggatgaacag tatgtcgagg ctggcggccc cggctcaccg aattttcata
     2281 aatcgaacgc cctgaaaacc atgcgtaagc agccgcagct gcaggggaaa ttgctggcga
     2341 aaggctcctg ggattatcaa ctggtggatg aactggtgcc gcagcctggc gatattgtgc
     2401 tgccgaagcc gcgctacagc ggtttcttca atacgccgct ggacagcatt ttgcgcagcc
     2461 gtggaataca ccatctggtt ttcaccggta tcgctaccaa cgtctgcgtc gaatcgacgc
     2521 tacgcgatgg cttttttctg gagcatttcg gcgtggtgct ggaagacgca acacaccagg
     2581 cggggccgga atttgcacag aaagccgcgt tgttcaatat cgaaaccttt tttggctggg
     2641 tcagcgacgt cgaaacgttc tgcgacacgc tttctcccac gtcctttgct cgtatcgctt
     2701 aaggagttta acgatgccaa aatccgtaat tattcccgct ggcagcagcg caccgctggc
     2761 ccccttcgtt cccggcacgc tggctgatgg cttggtgtat gtctccggta cgctggcttt
     2821 tgatcaacat aataacgtgc tgtttgccga tgacccaaag gcgcaaaccc gccacgttct
     2881 ggaaactatc cgcaaggtga tcgagacggc gggtggcacg atggcggatg tgaccttcaa
     2941 cagcatcttt attaccgact ggaaaaatta cgccgcgatt aacgaaatct acgccgagtt
     3001 ttttccgggt gataaaccgg cgcgattcta cattcagtgc ggactggtaa aacctgacgc
     3061 gctggtggaa atcgccacaa ttgcgcatat cgccaagtga ggccgcgatg aaactttcac
     3121 tctcacctcc cccttatgct gatgcacccg tagtggtgtt gatttcgggt cttggcggta
     3181 gcggcagtta ctggttaccg caactggcgg tgctggagca ggagtatcag gtagtctgtt
     3241 acgaccagcg cggcaccggc aataatcccg acacactggc agaagattac agtatcgccc
     3301 agatggcagc ggaactgcat caggcgctgg tagccgcagg gattgagcgt tacgcggtgg
     3361 tcggccatgc gctcggtgcg ctggtgggga tgcagctggc gctggattat cccgcgtcgg
     3421 taactatgct ggtcagcgtt aacggctggc tacgaataaa cgcccatacg cgccgctgtt
     3481 ttcaggttcg cgaacgatta ctgtatagcg gcggcgcgca agcatgggtg gaagcgcagc
     3541 cgttgttcct ctatcccgcc gactggatgg cggtccgcgc acctcgcctg gaggcagaag
     3601 acgcgctggc actggcgcat tttcagggca aaaataattt actacgtcga cttaacgccc
     3661 tcaaacgcgc tgactttagt caccatgcgg atcgcatccg ctgcccggtg caaatcatct
     3721 gcgccagtga tgatctgctg gtgccaacag catgttccag tgaacttcat gccgccctgc
     3781 ccgatagcca gaaaatggtg atgccctatg gcggacacgc ctgcaacgtg accgatcccg
     3841 aaacgtttaa tgctctgtta ctcaacgggc ttgccagcct gttacatcac cgtgaagccg
     3901 ccctgtaagg aattgctatg aacgaagccg ttagcccagg tgtgcttagc accctgttca
     3961 ccgatgcccg cactcacaac ggctggcggg agacacccgt cagcgatgag acgttacagg
     4021 agctttatgc cctgatgaaa tgggggccga catcagctaa ctgttctccg gcacggatcg
     4081 tgtttatccg cactgcagaa ggaaaagaac gtctgcgccc ggcgctttcc agcggcaatc
     4141 tgcaaaaaac cctgaccgcg cccgtcaccg ctatcgtcgc ttgggacagt gaattttatg
     4201 aacggttacc actactgttt ccccacggtg atgcccgcag ttggtttacc tccagcccac
     4261 aacttgccga agaaacagcg tttcgcaaca gttccatgca ggcggcctat ctgatcgtcg
     4321 cttgccgtgc gctgggactg gataccggcc cgatgtcggg ttttgaccgt caacacgttg
     4381 acgacgcctt ttttgcgggc agcacgctga agagcaatct gctgattaat atcggctatg
     4441 gcgatagcag caaacttttt gcgcgcctgc cacgtctgtc ctttgaagaa gcctgcgggc
     4501 tgttgtaagg agcaaactat gaacatagtt gatcaacaaa cctttcgcga tgcgatgtcc
     4561 tgtatgggcg cggcggtcaa tatcatcacc acggacggtc cagccgggcg cgccgggttc
     4621 accgccagcg ccgtctgcag cgtgaccgac acgccgccca cattactggt gtgcctgaat
     4681 cgtggggcgt ccgtctggcc ggtattcaat gaaaatcgaa cgctgtgtgt aaatacactg
     4741 agcgccgggc aggagccgct ttcaaatctt tttggcggca aaacgcccat ggaacaccgc
     4801 tttgccgccg cccgctggca gacgggtgtg accggatgcc cgcaactgga agaggctctg
     4861 gtttcgtttg actgccgtat cagccaggtg gtgagcgtcg gcacccacga cattctgttt
     4921 tgcgccatcg aagcgattca tcgtcacacc acaccctacg ggctggtgtg gttcgatcgc
     4981 agttatcacg cgctgatgcg ccctgcttgt taacctcctt aaggagacag ctgatggcaa
     5041 tgttcggttt tcctcactgg cagttaaaat cgacctctac agagagcggc gtggtcgcgc
     5101 cggatgaacg attgccgttt gctcagacgg cagtaatggg cgttcaacac gcggtggcga
     5161 tgtttggcgc gacggtgtta atgccgattc tgatggggct ggatcccaat ctttccattt
     5221 taatgtcggg gatcggcacg ttactgtttt tctttattac cggcgggcgc gtccccagtt
     5281 atctcggctc cagcgcggct tttgtcggcg tggtgattgc cgcgacgggc tttaacggtc
     5341 agggcattaa cccgaatatc agcatcgcgc tgggcgggat tatcgcctgt gggctggttt
     5401 ataccgtcat tggcctggtg gtgatgaaaa ttggtacacg ctggattgaa cgactaatgc
     5461 cgccggtggt gacgggtgcg gtggtgatgg cgattggcct gaacttagcg cctatcgcag
     5521 tgaaaagtgt atcggcttcg gcctttgata gctggatggc ggtaatgacg gtgctgtgta
     5581 ttgggctggt ggcggtgttt acccgtggca tgatccagcg gttattgatt ctcgtaggat
     5641 taattgtcgc ctgtctgctg tatggcgtga tgaccaacgt tttagggctg ggaaaagccg
     5701 tcgattttac tctcgtcagc tatgctgcct ggttcggtct gccgcatttt tccactcctg
     5761 gttttaatgg ccaggcgatg atgttgattg cgcccgtggc ggtgattctg gtggcggaaa
     5821 acttaggtca tctcaaggcg gtcgccggga tgaccggacg caatatggac ccgtatatgg
     5881 ggcgagcatt cgtcggtgat ggtctggcaa cgatgctttc cggctctgtc ggcggtagcg
     5941 gcgtcaccac ctatgctgaa aatatcggtg tgatggccgt gacaaaagtc tactcgacgc
     6001 tggtgtttgt tgcagctgcc gtcatcgcta tgctgttggg cttttcgccg aaatttggcg
     6061 cgctgatcca taccattccg gccgcggtga ttggtggtgc atcaattgtt gtgttcgggc
     6121 tgattgccgt cgcaggcgca aggatatggg tacaaaaccg tgtcgattta agccagaacg
     6181 gtaatttgat tatggtcgca gtaaccttcg ttctgggcgc gggtgatttt gctctcacgc
     6241 ttggcggttt tacgttggga gggatcggta cagcaacctt tggcgcgatt ttactcaatg
     6301 cgttattaag cagaaggttg gttgacgttc caccgccaga agttgttcat caggagccat
     6361 aaacagaaaa ccgccgggtt accggcggtg atgacttttt gacgagctta agaaaaaaat
     6421 gcattcgtca ttgattagcc cggctagttg tcgcttttgc cgtgactgct cttgccacct
     6481 tttttacctg cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga
     6541 tggtttgcca ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg
     6601 gcgtatggcg gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggc
     6661 agacccgcgt ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa
     6721 taattatcca taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt
     6781 ttgcgacaaa attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt
     6841 tgcactctga aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga
     6901 gataacacga ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg
     6961 gacatattga aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtg gatggtgcag
     7021 aagttgtcgt taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg
     7081 gtaaaacgca aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta
     7141 tttttggtac acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc
     7201 agacgggcgg cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt
     7261 ccaccggtac tggcggcggt caggaacaaa ccatcacatc cacctggacg acccttgcgc
     7321 atcacggcat ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac
     7381 aggttcgcgg cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc
     7441 agccaagcca ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg
     7501 cagttaaact taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa
     7561 agctcatcac gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc
     7621 cattttcgaa gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag
     7681 tgatgaagtg ttagtcaaag cctttgctaa gaccgataaa gattcgcttt tttggggcga
     7741 acagaccatc gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt
     7801 ttctgttatt tcaccgcctc attcaacacg ctatcaaact tatccatcgg gcagaaaccg
     7861 ttggcatcta tcgggcaacc gcttaattcc agcgtcacgc gctgcgcagg tgcctgcagg
     7921 gttaacgcat cggcattacg taactgttcc gcactctggt acacatattc aattttcatc
     7981 aaatcgcgat tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc
     8041 gtgcgttcgt tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag
     8101 gctatgttgg agtcgtgccc aactaacacc gtgattttcg gagcactggc gcgatcggtg
     8161 accagggctt tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt
     8221 gaggtaaaca gactgtcctg gtaaccgttt ttcagcttcg acaatacctt ccactgctgg
     8281 tcagatttga tttctcccca ggccacctga tccatcggaa aaccttcgta gtattgcaaa
     8341 gtaaacgcat ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt
     8401 tgatacttgg cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta
     8461 caggcagggg aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc
     8521 tggagtttgc tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa
     8581 tcatcggtga tcatcgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc
     8641 acaggaatat cacacccagg gaatgcacca gtaataaaga actgggcggt agcgacggta
     8701 cgttgcagac tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc
     8761 actatcccct gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg
     8821 ccacctttgg tggtgagttg cccaccgggg acgtcccatt cgggccattt attcggcgtc
     8881 gactgctcca gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc
     8941 atgagcactt gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg
     9001 agtaaaacta tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct
     9061 tttgttatca atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg
     9121 caaaatggct tgttgaacgc ctgtttttct atatacatct tcaaaaacag gcaaggtttg
     9181 acgcaaacta tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg
     9241 ctaaacagaa tatctgcggc gttaccaata cagagacaat atttaccaat aaaaaagtgt
     9301 gagactaata acaatggttt ttagcatatg cctttgccat taagttgcat aacgatttaa
     9361 atagtcaagt tgcgcattca tttccttatt ttttaactta taagcctgag aaaaaagttc
     9421 aggagggagg taaacatcat aatgattgcc agtgtttatt aaataaccat actcaagatc
     9481 ttcatctgtt gaaaaggatt catttcccaa ggagattgat gccacattac tattaacaat
     9541 aaataatgag caaatgctat ctgaaagaac agcaagaaaa ttcttcaccg ctttaatcct
     9601 atccgacgtg tcggtatcta aagcaagcaa attcttgtta tcattaaata taaactcggg
     9661 taaatgcagt atgcattttt tgtcattcaa gcaactatca atgaattgtt ttttaaaaaa
     9721 cagaaagctc catgacataa aactacccac accctgaatt ttgttgtcag attgcagatc
     9781 aacgccaaat aatgtcttta catcttccct gagattatag aatgccgtat caacaagagc
     9841 ctcgatcgtt tgtttatcaa caattttaat aaactcttcg tatacttgct tttgcgtctt
     9901 atggctgccc agcgcccatg aatactcatg ttcttttaaa accagtatgg ctcttaacag
     9961 acaatatcca tcgccatcta ctggagcgta ttttaatcca tatgttttat taatttcact
    10021 ctgaataaaa gatatgccag acaaatcaat accagtatct aaatcagata ttctcggcat
    10081 atttatctgt tctggctgga tactcatatt ttcatcatct aaacaatact cagaaaaaac
    10141 atcatcacac gcattaatgt tttcgatata ttccataaag ccataccaat aataaccttt
    10201 aaaatctgtg atagactctg aacctctgcc cggcaaggac actctaacaa tatcaacatc
    10261 ttgaacatat tgtatagtat atgtaatacc tcctgaaacc cctgaataaa tttgtgtatc
    10321 attagtacgc aatgttctca taaataatga tagatcatta tcattctgca tttcctcaaa
    10381 ttctttttct agtgattcac attccagaca tggagtatcc tcttcattta ctttacgtct
    10441 gagttcattg tacaaccgca taaaacacac cagtgtgttt tcaaaaagcg tgattgaata
    10501 tgtttgattt ttttcgtact cattttttat atcgttccag gcctgtttca aataattatt
    10561 tgcagtgcaa ctcattccat gtatattact acccatatat agcgtctata aaatttaata
    10621 aataatgacg ccctagttaa acttaaagtg cctggttcaa ctatcaaaaa tcgctcaccc
    10681 tttttcacct gtttaaaata tgttcagcaa cccatcttga tggcgacctc ctctccgcga
    10741 tgatttcaat aacatattct gtgttggcat atgaaatttt gaggattacc ctacacttat
    10801 aggagttacc ttacaggggt tccttcaatt tgtgttgatt tacgcgagat aacgctatgg
    10861 aattaaagga ttattacgcc atcatgggcg tgaaaccgac ggacgatctc aagacaatca
    10921 agaccgccta tcgtcgactg gcccgtaaat accatcctga tgtcagcaaa gaaccggatg
    10981 ccgaagcccg cttcaaagag gtcgctgaag cctgggaagt gttaagtgat gaacaacgtc
    11041 gcgctgagta tgatcagatg tggcaacatc gcaacgatcc gcaatttaac cgtcagttcc
    11101 atcatggcga cggtcagagt tttaatgccg aagattttga cgatatcttc tcgtcaattt
    11161 tcggtcagca tgcccgccag agccgtcaac gccccgccgc acgcggccac gatattgaaa
    11221 tcgaagtggc ggtattcttc gaagaaacgc ttactgagca taagcgtacc atcagctata
    11281 acctgccggt ttataacgcc tttggcatga tcgaacagga aatcccgaaa acgctgaatg
    11341 tgaagatccc ggcaggcgtc ggcaatggtc aacgcatccg cctgaaaggc caggggacgc
    11401 cgggcgaaaa cggcggtcca aatggcgatt tgtggctggt gattcatatt gcgccacatc
    11461 cgctgttcga tattgtcggc caggatctgg aaattgtggt gccggttagc ccgtgggaag
    11521 cggcgctggg tactaaagtc accgttccaa cactgaaaga aagcattttg ctgactatcc
    11581 cgccaggcag ccaggccggg caacgattgc gcgttaaagg caaaggtctg gtgagcaaaa
    11641 aacagaccgg cgatctgtat gcggtactga aaatcgtgat gccgccgaaa ccggatgaaa
    11701 acactgccgc gctgtggcag caactggcag acgcccagtc gtcttttgat ccacgtaaag
    11761 attgggggaa agcataatgg ctaatgttac ggtgactttt actattaccg aattttgcct
    11821 gcataccggc atctctgaag aggagttgaa tgaaattgtc ggtttggggg tggttgaacc
    11881 gcgtgagatc caggaaacaa cctgggtatt tgacgaccat gccgccattg tggtgcaacg
    11941 cgcggtacgc ctgcgtcatg aactggctct ggactggccg gggatcgcgg tggcgctgac
    12001 gttaatggat gatattgcgc acctgaagca ggaaaaccgc ctgctacgcc agcggctttc
    12061 ccggtttgta gctcatccgt gatgtgtgtt tgccggatgt gatgacgcat ccggcaactg
    12121 cgccataatg cctgatgcga cgctaaacgc gtcttttcag gcgcataaac gacgttatct
    12181 gttttggtgg tcgcactcca ctaacaccag caataactgg cttagtgccg cgtaaaaacc
    12241 aaagctgtca tactgtcgac aacgcgcagc aaactctggt aaccattgcc gtagcgccgt
    12301 cagtattttt tgccgcaaac tgtcgattct tcgcgcagga acggtcccct ctcccagcga
    12361 aaaatgcaga taactcaaca actcgagata gatcgccaga tgatctgccg gttcgttgaa
    12421 attgccactg gtttccatcc ctgcctcaac taacaagcgt ttaatctctt gctcgtcctg
    12481 tttgtaggcc gatgcatacg gcagcgccgc ttgtttgtcg gtcatcagaa acaggccgca
    12541 aaagtcagcg gccagttcca gacgggcatc gtcacgtact gtcagcgtg
//
LOCUS       DAJTYI010000113        12452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYI010000113 DAJTYI010000000
VERSION     DAJTYI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(83..1174)
                     /gene="ychF"
                     /locus_tag="OO564_003089"
     CDS             complement(83..1174)
                     /gene="ychF"
                     /locus_tag="OO564_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6654128.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1291..1875)
                     /gene="pth"
                     /locus_tag="OO564_003090"
     CDS             complement(1291..1875)
                     /gene="pth"
                     /locus_tag="OO564_003090"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6654129.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2153..2431
                     /gene="ychH"
                     /locus_tag="OO564_003091"
     CDS             2153..2431
                     /gene="ychH"
                     /locus_tag="OO564_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6654130.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2486..4165)
                     /gene="dauA"
                     /locus_tag="OO564_003092"
     CDS             complement(2486..4165)
                     /gene="dauA"
                     /locus_tag="OO564_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6654131.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4290..5237)
                     /gene="prs"
                     /locus_tag="OO564_003093"
     CDS             complement(4290..5237)
                     /gene="prs"
                     /locus_tag="OO564_003093"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6654132.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5388..6239)
                     /gene="ispE"
                     /locus_tag="OO564_003094"
     CDS             complement(5388..6239)
                     /gene="ispE"
                     /locus_tag="OO564_003094"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6654133.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6239..6862)
                     /gene="lolB"
                     /locus_tag="OO564_003095"
     CDS             complement(6239..6862)
                     /gene="lolB"
                     /locus_tag="OO564_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6654134.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7076..8332
                     /gene="hemA"
                     /locus_tag="OO564_003096"
     CDS             7076..8332
                     /gene="hemA"
                     /locus_tag="OO564_003096"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6654135.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8374..9456
                     /gene="prfA"
                     /locus_tag="OO564_003097"
     CDS             8374..9456
                     /gene="prfA"
                     /locus_tag="OO564_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6654136.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9456..10289
                     /gene="prmC"
                     /locus_tag="OO564_003098"
     CDS             9456..10289
                     /gene="prmC"
                     /locus_tag="OO564_003098"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6654137.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10286..10678
                     /locus_tag="OO564_003099"
     CDS             10286..10678
                     /locus_tag="OO564_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6654138.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10682..11491
                     /locus_tag="OO564_003100"
     CDS             10682..11491
                     /locus_tag="OO564_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6654139.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11527..12381
                     /gene="kdsA"
                     /locus_tag="OO564_003101"
     CDS             11527..12381
                     /gene="kdsA"
                     /locus_tag="OO564_003101"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6654140.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3099 a         3156 c         3244 g         2953 t
ORIGIN      
        1 aaaatccacg caattgcgtg gattttatat acttttgcgc tcttcatgag atttagcgaa
       61 acctcatgag acaataaatt aattagacgt tgaaaaggaa gttcatcaca tcgccatctt
      121 tcacgatgta atctttacct tctgcacgca ttttgcctgc ttctttcgcg ccttgttcac
      181 ctttgtaagt gatgaaatct tcaaacgaga tggtttgtgc acggataaag cctttttcaa
      241 aatcagtatg gattttgccc gctgcctgcg gcgcggttgc tccaaccgga atggtccatg
      301 cacgcacttc tttcacccca gcggtgaagt aagtttgcag gttcagcagc ttataaccgg
      361 cacggatcac acggttcagg cccggttctt ccagcccaag ctcctgcata aactcgtcac
      421 gttcttcgtc gtccagttcg gcaatgtctg cttcaacagc agcacaaacc ggaaccacaa
      481 cagaaccttc tttcgccgcg atttcacgca cctggtcaag atatgggttg ttttcaaaac
      541 cgtcttcgtt gacgttggct atgtacattg ttggtttcag cgtcaggaag ctcaggtaac
      601 gaatagccgc tttctcttca gcgcttaaat ccagcgcgcg cagcatacct gcgttttcca
      661 gctggggcag gcatttttcc aggaccgcca gctcagcttt cgcgtcttta tcgccacctt
      721 tggctttctt ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca
      781 gttcggtgtt gataacttca atatcgtcag ccgggttaac tttgccggaa acgtgaatga
      841 tgttgtcatt ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg
      901 tcaggaactg gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat
      961 cgacaaattc catggtcgtg ggaagcgtac gttgcggttt tacgatttca gccagttgat
     1021 ccaggcgagg gtcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa
     1081 agttggccgc ttcaataccg gctttggtca gcgcgttgaa cagggtagat ttcccgacgt
     1141 tgggcaaacc gacgataccg catttgaatc ccatgattta aatcacctta atatcttaat
     1201 aatcaacctg ttattgctaa cagattgcag aaatggaaat aactttgcct attatacacg
     1261 gcactcggca aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt
     1321 cgttgctttg gtcaagccat ctgtaaacca catttcagta caacgcgccg cttcgtcgat
     1381 ggcttcatca attaacttct gttcactaac aggcggtttg cctaacacaa aaccgacaac
     1441 tttattttta tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt
     1501 acccaattta ctgatgatgt ctttcagtcc attgtgacca ccatggccac cgcccaactt
     1561 aaatttggcg acgccaggag gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg
     1621 attaatgcgg aaaaaactgg ccatcgccgc aacggctttg ccgctgagat tcataaatgt
     1681 agtcgggact aacaggcgga catcttcgcc tccaagggtg actcgcgaag tataaccaaa
     1741 gaatttagcc tcttcgcgca gcggagcgcg caaacgctct gccagtaagt caacgaacca
     1801 ggcaccagca ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat
     1861 caatttaatc gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg tagtttactg
     1921 gttgcggccc cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa taattgcctg
     1981 agtggactat tagaaagtca aggtgttcag gcgtttattt gtaaagtttt gttgaaataa
     2041 gggttgtaat tgtgattacg cccgcacata acccacaggg tgttgtctat actttacaca
     2101 taaggaagag gggtattccc tgttacaacc cagaaagttc cggaggtgac atatgaaacg
     2161 caaaaacgct tcgatactcg gtaacgtgct catggggttg ggtctggtgg taatggtggt
     2221 cggcgtgggg tattcaatcc tcaaccagtt accacagttt aatatgcccc agtatttcgc
     2281 acatggtgca gtgctaagta ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt
     2341 tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt cgtcactatg acaaacgttg
     2401 ccgccgtagc gataatcgcc gtcatagcta acaaataatg cagtttgctg accagtcagt
     2461 ttgcgctgac tggtcaatct cgtacttata aatccgccat cgccgcgcga cgattcggga
     2521 agaacgcgag gcgtcccggg atcggttgaa tgcctgcgcg agccatagtt cgcagtggct
     2581 ggaattccac gttgcacacg cgcagttcac atccttcggg cagacgcttc acaaaacgct
     2641 ggaacgcatc aagaccacca gcatcaagta ccggaacggc atcccacttc agaatcacaa
     2701 tccgtttgcc ttcaagacgt gactccaggt ccgtgaataa gccttcagca gcagcaaaaa
     2761 acagcgggcc aataacgcgc agaaccagga catcgtctgg aacatctacg actaccggtg
     2821 ccaggcgagt catacgtgcg atacgacgca taaacagcag cgatgccagc acgatcccca
     2881 cgctgatggc aataaccata tcaaacagca cggtcagtga catacacagc agcatgacga
     2941 tgatgtcatc tttcggcgca tgacgcagca agtcgaccac tttgtgcgct tcactcatgt
     3001 tccacgccac catcaacagc agggctgcca tggcggaaag cggcagccag gagagcagtg
     3061 gtgccagtac cagcagggcg agaataacca gaatagagtg gatcaccgcc gagatagggg
     3121 aagttgcccc ggcacggacg ttagcggcag aacgcgcgat ggcagcagta gcggtaatac
     3181 caccaaagaa tggagcgatg atattcccca gcccctgacc gaccagttca ctattcgcct
     3241 tgtgcttagt cccggtcata ccatccagta ccacagcgca gagcagagat tcgatcgccc
     3301 cgagcattgc cattgagaat gccgcaggca gcagggtgcg aatagaatcc caggttagcg
     3361 tgaattctga attaggcaga tcccacggca gcaccagttg cggcagcagt tgcggaatac
     3421 cgttaccctg agaaccatcg gccaggacgt agtggaattg cgaaccgatg gtagcaacat
     3481 gtccgccgag caggttaaca atccccatca ccgcgcaacc agccagcaag gccggaaggt
     3541 gaccgggtaa acgaatgccc agacgcggcc agaaaaccag aatgccaagc gtcacaatgc
     3601 caatggcagc atcacccaca ttaatggtcg gcagcgccat aaataatgcg ccgacttttt
     3661 gtagataatg ttccgggaca tgggccattt gcagaccgag aaaatcttta atctgcatgg
     3721 taccgatggt gatcccgata cccgaggtga aacctaaggt gacggaaacc ggaatatact
     3781 caatcagacg accaaagcgt gccagcccca tcagaatcaa aaagattccc gacaacaagg
     3841 tcgcaaccag cagtcctgcc agtccaaact gttgcgaaac gggatagaga attaccacaa
     3901 atgccgcagt cggaccggaa acgctaaagc gtgacccacc cgttagagca atgacaatcc
     3961 ccgcaacagc tgcggtatat aaaccgtact ggggtgccac accactacca atagccaacg
     4021 ccatcgccag cgggatagca ataatcccga cggttatccc ggcaatcagg tcacgggtaa
     4081 accgtgcggc agtatatttt tctttccagc aagcgtcgat cagagcgcgg aaaggcatca
     4141 catgtgagga aaatattttg ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa
     4201 ctaaatggca ggtgaaggag gcataggtca tacaaatgga tattacagac aaaaaaaccc
     4261 gccgcagcgg gtctttgagc cgggttcgat tagtgttcga acatggcaga gatcgattct
     4321 tcgttgctga tacgacgaat cgcttcggcc agcatacctg acagggtcag agtacgcacg
     4381 ttcggcagtg atttgatttc atcgctcagc ggaatggtat cgcagacaac gacttcatca
     4441 attacagagt tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac
     4501 gcaaatacac gtttagcacc acgttctttc agcgcttcag cagctttaca cagcgtaccg
     4561 ccagtgtcga tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc accgatgata
     4621 tgcatcacct gggaaacgtt cgcacgtgga cgacgtttgt cgatgattgc catatcggta
     4681 tcgttcagca gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc
     4741 acaattgggt tatccagatt cagctgcagc atgtcttcca gcaggatcgg gctaccaaat
     4801 acgttatcaa ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc
     4861 agcacacggt caacaccgac gctggagagg aagtctgcaa ccactttcgc agtgattggt
     4921 acacgagcgg aacggacgcg acggtcctgg cgcgcatagc caaagtaggg gataacagcg
     4981 gtgatacgac ctgcggaagc acgacgcagg gcatcaacca taacgactaa ttccatcagg
     5041 ttgtcgttag taggggcaca agtggactgg atgatgaaaa tatcaccacc gcgtacattt
     5101 tcgttaattt gtacgctgac ttcgccgtcg ctaaagcgac ctacagcggc gtcgccgagt
     5161 gaagtgtaca ggcggttggc aatacgttgt gctagtaccg gggtggcgtt accagcaaaa
     5221 agcttcatat caggcacgag aagaacctca ggcatgcgtc cattggtgga aagaatctgc
     5281 cgaaaactgt gcgggccagg catgatcctt tccaggcggt gtattaaaga gcgcgatgca
     5341 acgtctggaa caaggtgacg ttgtcaccga aactcagctt gcccggctta aagcatggct
     5401 ctgtgcaatg gggaaagatt aacgcctttc gccacaaagc cattgagcca ttccggggct
     5461 tgctctagca cctggcgggc ttcagactct gtatcaaatt cagcaaagac acaggcccct
     5521 gtcccagtca ggcgcgacgg ggcgtatttt aacagccagg aaagcaccgc atcaacctcg
     5581 cgaaaacgtt ttcttgcgat aacctcgcaa tcattgctga attcacattt tagcaacgtt
     5641 tctattgacc tttttggcgt attgcgcggg agttcaggat ctttaaaaat caccggagtc
     5701 ggaatactta caccagggtg cgccaccaga taccacttct ctggcggatc caccggcgtt
     5761 agtatttcac caacgccttc ggcaaacgcg gcatgccccc gaacaaagac aggaacatct
     5821 gcgcccagcg tcagccccat ttccgccagc tcatccatgc ttagcccgca ttgccagaga
     5881 tgatttaatg ccaccaggac cgtcgcggca ttggatgaac caccgccgag accgccgccc
     5941 atcggcaaac gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag acgcccgctg
     6001 tctgccgcag ttttcatcaa caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc
     6061 acgccttcaa cgggcgttaa cagacgaata tccccatcgt cacgaagctc aatgctgatg
     6121 gtgtcgccgt aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc
     6181 tgaccggtaa tgtataaaaa cagattaagt tttgccggag agggccactg tgtccgcatt
     6241 atttcactat ccagttatcc atttttaact tgatgcgttg accaccgtcg gtgagttcca
     6301 tattggttgg catcgcaggt tgcgttttgg tgtcataacc accataaaca accttccagt
     6361 ttttgccatt ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc agtttgtagt
     6421 cggttgcatc acccggtaaa cctaaaatcc actggcgcaa gctgttgagc ggaattggca
     6481 ttccggtcaa tttgccaatc atctcttcgg cgtcatcggc ggtataacgc tgacctttat
     6541 tgtcgactaa ctgcacgtta cccggttgag cattcagctc cagttccgtg ctgcccaatg
     6601 ggttagtgag cagcagacgg tagcgatcct ggccggtttg ctgccagaaa aagcgggcgt
     6661 acactttttg ttggtcagaa atataagcga acgcgccgcg agtctgatac tgattaagat
     6721 tgcgcacgtc ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg ccaggacctt
     6781 tgggcgtggt aacggaacag gcagtgagca caagagcagc cagcggtagc aggcggataa
     6841 gacgaaaatc gggcaggggc atagtgatga caagtccttg agatacgttg cagttataac
     6901 ccttaatgct agcgttaccg tccgctatcg tctatgttca agttgtctta attgccagaa
     6961 tctaacggct ttcggcaatt actccaaaag ggggcgctct cttttattga tcttacgcat
     7021 cctgtatgat gcaagcagac taaccctatc aacgttggta ttatttcccg cagacatgac
     7081 ccttttagca ctcggtatca accataaaac ggcacctgta tcgctgcgag aacgtgtatc
     7141 gttttcgccg gataagctcg atcaggcgct tgacagcctg cttgcgcagc cgatggtgca
     7201 gggcggcgtg gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg ttgaagagca
     7261 ggataacctg caagaggcgt taatccgctg gctttgcgat tatcacaatc ttaatgaaga
     7321 agatctgcgt aaaaacctct actggcatca ggataacgac gcggttagcc atttaatgcg
     7381 tgttgccagc ggcctggatt cactggttct tggggagccg cagatcctcg gtcaggttaa
     7441 aaaagcgttt gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg aacgcatgtt
     7501 ccagaaatct ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg gtgccagtgc
     7561 tgtgtctgtc gcttttgcgg cttgtacgct ggcgcggcag atctttgaat cgctctctac
     7621 ggtcacagtg ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg
     7681 cgaacataaa gtacagaaga tgattatcgc caaccgcact cgcgaacgtg cccaaattct
     7741 ggcagatgaa gttggcgcgg aagtgattgc cctgagtgag atcgacgaac gtctgcgcga
     7801 agccgatatc atcatcagtt ccaccgccag cccgttaccg attatcggga aaggcatggt
     7861 ggagcgcgca ttaaaaagtc gtcgtaacca accaatgctg ttggtggata ttgccgttcc
     7921 gcgcgatgtt gagccggaag ttggcaaact ggcgaatgct tatctttata gtgtggacga
     7981 tctgcaaagc atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga
     8041 aactattgtc gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag
     8101 cgaaaccatt cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt taaccgccaa
     8161 agcgttagcg gcccttgagc agggcggcga cgcgcaagcc attatgcagg atctggcatg
     8221 gaaactgact aaccgcttga tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga
     8281 cggggataac gaacgcctga atattctgcg cgacagcctc gggctggagt agcagtacat
     8341 cattttcttt ttttacaggg tgcatttacg cctatgaagc cttctatcgt tgccaaactg
     8401 gaagccctgc atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc
     8461 gccgaccagg aacgttttcg cgcattatca cgcgaatatg cgcagttaag tgatgtttcg
     8521 cgctgtttta ccgactggca acaggttcag gaagatatcg aaaccgcaca gatgatgctc
     8581 gacgatcctg aaatgcgtga gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc
     8641 gagcaactgg aacagcaatt acaggttcta ttactaccaa aagatcctga tgacgaacgt
     8701 aacgccttcc tcgaagtccg tgccggaacc ggcggcgacg aagcagcgct gtttgcaggc
     8761 gatctgttcc gtatgtacag ccgttacgcc gaagcccgcc gctggcgggt agaaatcatg
     8821 agcgccagcg agggtgaaca tggtggttat aaagagatca tcgccaaaat tagcggtgat
     8881 ggtgtgtatg gtcgtctgaa atttgaatcc ggcggtcatc gcgtgcaacg tgttcctgct
     8941 acggaatcgc agggtcgtat tcatacttct gcttgtaccg ttgcggtaat gccagaactg
     9001 cctgacgcag aactgccgga catcaaccca gcagatttac gcattgatac tttccgctcg
     9061 tcaggggcgg gtggtcagca cgttaacacc accgattcgg caattcgtat tactcacttg
     9121 ccgaccggga ttgttgttga atgtcaggac gaacgttcac aacataaaaa caaagcgaag
     9181 gcgctttcag tgctcggtgc tcgcatccac gctgctgaaa tggcaaaacg ccaacaggcc
     9241 gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg taaccgtact
     9301 tacaatttcc cgcaggggcg cgttaccgat caccgcatca acctgacgct ctaccgcctg
     9361 gatgaagtga tggaaggtaa gctggatatg ctgattgaac cgattatcca ggaacatcag
     9421 gccgaccaac tggcggcgtt gtccgagcag gaataatgga atatcaacac tggttacgtg
     9481 aagcaataag ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg
     9541 agcatgttac cggcagaggg cgtactttta ttctcgcctt tggtgaaacg cagctgactg
     9601 acgaacaatg tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt gaacccattg
     9661 ctcatttaac cggggtgcga gaattctggt cgttgccgtt atttgtttct ccagcgacct
     9721 taattccgcg cccggatacg gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac
     9781 aaccttgccg tattctcgat ctcgggacgg ggaccggggc gattgcgctt gcgctggcta
     9841 gcgagcgccc ggactgcgaa attatcgctg tagatcgtat gcctgatgct gtctccctgg
     9901 cacaacgtaa tgcccagcat ctggcgatca aaaatatcca cattctgcaa agcgactggt
     9961 ttagcgcgct agccgggcag cagtttgcga tgattgtcag caatccgccg tatattgacg
    10021 agcaggaccc acatcttcaa caaggcgatg tccgctttga gccgctcact gcgctggttg
    10081 cggcagacag tggaatggca gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg
    10141 tatccggcgg ctttctgctt ctggaacatg gctggcagca gggcgaagcg gtgcgacaag
    10201 catttatcct cgcggggtat catgacgtcg aaacctgccg tgactatggt gataacgagc
    10261 gcgtaacgct cggccgctat tatcaatgac aagtttttct acactgctta gtgttcatct
    10321 tattagtatc gcgctttctg ttgggctatt aaccttacgt ttctggctac gttatcagaa
    10381 gcatccacag gcatttgctc gctggacgcg cattgtgccg ccggttgtcg atacggtgtt
    10441 attgttaagc ggcattgcgt tgatggctaa agcgcacatc ctgccatttt ccgggcaggc
    10501 acagtggctg actgaaaagc tgtttggagt tatcatttat atcgttttgg gttttattgc
    10561 actcgattat cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc cgctggcgtt
    10621 ggtggtgctg tacatcatca ttaaactcgc caccacaaaa gtaccgttac tggggtaagt
    10681 tatgagatcg ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct
    10741 ggcttgcgaa gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg aactggagcg
    10801 tctcgttagt ctggcgaagg aagaaatcag ccagcttctg cctttagaag agcagctgga
    10861 aaaactgatc gcgctgtttt acggcgactg gggatttaaa gcctcacgcg gcgtttatcg
    10921 tctttccgat gcattatggc tggaccaggt gttaaagaat cgacagggca gtgcggtatc
    10981 attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat
    11041 tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa
    11101 tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat
    11161 cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat
    11221 ccgcaaattg ctggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc
    11281 gttacgcacc agcgaagctt tattacaatt caaccctgaa gatccctatg aaattcgcga
    11341 tcgcgggttg atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta
    11401 tttcgttgaa cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa
    11461 catcgcgcat aaacatattg tgctgcatta attaatcgac attttactca agattaaggc
    11521 gatcctatga aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg
    11581 ttcgtactgt ttggcggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc
    11641 gagcactacg taactgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt
    11701 gacaaagcca accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg
    11761 aaaatcttcc aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa
    11821 ccaagtcagg cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt
    11881 gctcgccaga ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag
    11941 aaaccacagt ttgtcagccc ggggcagatg ggtaatatcg ttgataaatt caaagaaggc
    12001 ggcaacgaaa aagtgattct ttgcgatcgt ggtgctaact tcggctatga caacctggtt
    12061 gtcgatatgc tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc
    12121 gacgtgaccc acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt
    12181 gctcaggtgg ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt
    12241 gaagcgcatc cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct
    12301 aaactggaac cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa
    12361 gaactggata ccagcaagta atcttttttg cttgaaaaat aaagtcttag cgttctgcgt
    12421 taagactttt ttcatgggtg ccagatacaa aa
//
LOCUS       DAJTYI010000114        11752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYI010000114 DAJTYI010000000
VERSION     DAJTYI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(36..1400)
                     /gene="tdcG"
                     /locus_tag="OO564_003102"
     CDS             complement(36..1400)
                     /gene="tdcG"
                     /locus_tag="OO564_003102"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6654141.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1472..1861)
                     /locus_tag="OO564_003103"
     CDS             complement(1472..1861)
                     /locus_tag="OO564_003103"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6654142.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1875..4168)
                     /gene="tdcE"
                     /locus_tag="OO564_003104"
                     /pseudo
     CDS             complement(1875..4168)
                     /gene="tdcE"
                     /locus_tag="OO564_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(4202..5410)
                     /gene="tdcD"
                     /locus_tag="OO564_003105"
     CDS             complement(4202..5410)
                     /gene="tdcD"
                     /locus_tag="OO564_003105"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6654143.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5436..6767)
                     /gene="tdcC"
                     /locus_tag="OO564_003106"
     CDS             complement(5436..6767)
                     /gene="tdcC"
                     /locus_tag="OO564_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6654144.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6789..7778)
                     /gene="tdcB"
                     /locus_tag="OO564_003107"
     CDS             complement(6789..7778)
                     /gene="tdcB"
                     /locus_tag="OO564_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6654145.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7877..8815)
                     /gene="tdcA"
                     /locus_tag="OO564_003108"
     CDS             complement(7877..8815)
                     /gene="tdcA"
                     /locus_tag="OO564_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6654146.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9005..9349
                     /gene="tdcR"
                     /locus_tag="OO564_003109"
     CDS             9005..9349
                     /gene="tdcR"
                     /locus_tag="OO564_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6654147.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9605..10144
                     /locus_tag="OO564_003110"
     CDS             9605..10144
                     /locus_tag="OO564_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654148.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10166..11342
                     /locus_tag="OO564_003111"
                     /pseudo
     CDS             10166..11342
                     /locus_tag="OO564_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         3318 a         2850 c         2551 g         3033 t
ORIGIN      
        1 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
       61 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
      121 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc
      181 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
      241 tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca
      301 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc tgccattgaa
      361 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
      421 ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt
      481 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
      541 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
      601 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctagaaacc
      661 agctgacgat gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct
      721 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaaccct
      781 gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata
      841 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
      901 acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg
      961 ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc
     1021 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     1081 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     1141 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     1201 ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc gtacagatcg
     1261 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     1321 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     1381 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     1441 acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     1501 tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg
     1561 tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca
     1621 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     1681 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     1741 caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa
     1801 cgccctgaac ataagggccg attgcgccta gggcacgttg cgtttcgata atctttttca
     1861 tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt
     1921 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     1981 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     2041 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     2101 aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg
     2161 tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     2221 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     2281 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     2341 gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc
     2401 ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     2461 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     2521 ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg
     2581 ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag
     2641 ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc
     2701 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc
     2761 agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     2821 gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     2881 atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa gtcagtacgc
     2941 atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     3001 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc
     3061 ggccccatag tgtgcaggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac
     3121 cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg
     3181 aatcaaattc cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga
     3241 tcagttcctg tgcctgctgc tcattgagga cgccagcttt aaagtcgcgc tcaatgtaga
     3301 tatccaggaa cgatgccgtg cgccccagcg acatcgcgcc gccattttgc gatttcactg
     3361 ccgccagata agcgaagtag agccactgca ccgcttcctg tgcattctgc gccgggcgag
     3421 agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct
     3481 ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag
     3541 actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataactgatg ccatacagcg
     3601 ctacgcggcg atagtcaccg ataatgcgcc cacgaccata gccatctggt aaaccggtca
     3661 gcacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt
     3721 tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat
     3781 aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca
     3841 acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat
     3901 catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt
     3961 tttcgatacg gatgccttcc attacttttt ctcacaattc cgtggtggca ggcgtcgctt
     4021 cggcgagaaa agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga
     4081 cattaatttc gcttttccag tccgtacctt taaagccaag ccatgcgtca gcgtacagct
     4141 tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa
     4201 attatgcaaa ttctgcgggc gcgttaactt tacctaaatg aatggcatcc aaagcaatca
     4261 ttttttcttc gttagtcgga ataacgacgc aaatgacatg cgcattttca ctggaaacaa
     4321 ttcgctcgcc aaaagagtta gatcgattat tcatttctgt atcaatctct acgcctaata
     4381 cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct attccgccgg
     4441 tgaatataat tccatccagc cgacgtaatg aagctgcatg tccggcaata tgacgggcaa
     4501 ttcggtgaac aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt
     4561 tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat
     4621 tcactacgcg ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg
     4681 caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc
     4741 ccattgaggt gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa
     4801 gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcagcgaa tgtgcgcgct
     4861 gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat
     4921 aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg
     4981 tatcaaatac ggccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac
     5041 ttaaatttgc gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga
     5101 cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc
     5161 caattaaggc cacgctgtca tttaaattcc gtttttccag ttcaaatgca attgccttca
     5221 atgcaccttc gtagctgtgg agagccagcg gtgctggctc tcccccattt acggataaga
     5281 atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg
     5341 catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa
     5401 attcattcat ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc
     5461 aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc
     5521 gccagagacg gcgctttacg gatagcatac atcggcaaca ggcacagcag ggatgcgata
     5581 atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc
     5641 caggtggagc ccatgatgaa gatcatgctg agagtattca gtttacccag cgacactttg
     5701 gttttgtcac ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc
     5761 agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat
     5821 tccagtgtaa tcgcgaacgt tgttttcgta ccggccatgg acgcaaagtg gttagccaga
     5881 taagaaagca ctggaatatt ctgcactttg gcttccgcca tgttggccgg agacagagta
     5941 aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa
     6001 atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca
     6061 cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg
     6121 gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg
     6181 aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc
     6241 accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta
     6301 ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc
     6361 agcggtgcaa atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg
     6421 ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg
     6481 ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca
     6541 gaaagacaca gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc
     6601 aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc
     6661 ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag
     6721 gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa
     6781 cgaattaatt aagcgtcaac aaaaccggtg atttgagaga cgcgagaaag atcgatattg
     6841 ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt
     6901 aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt
     6961 aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat
     7021 tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg
     7081 gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga
     7141 acgttttctg actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc
     7201 gcaataccag caattaaacc accaccacca attggcacaa tcacgttatc gacatcatag
     7261 agatcttcca taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg
     7321 taaggtggga taaaaatacg accttccatt tcgacaattt cgctcacttt agcgatagtg
     7381 tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact
     7441 ttggattttg gcgcaccttt tggcatcacc actttaccgt cgatacccag tatcgcgcag
     7501 gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt
     7561 tccgcatcgg tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta
     7621 cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg
     7681 gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata
     7741 tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat
     7801 taaatatgta ggtaaccgta accgacacct gcaaaacaga caggtggatt atttatgttt
     7861 aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac ccattataag
     7921 atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt
     7981 tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa
     8041 attgattaga accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat
     8101 tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga
     8161 tgccatttct ttgtaacgta gtaagcagtt cgctgtagta ccccatatta gtttgtggca
     8221 acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact
     8281 tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct
     8341 tcatttctgc acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta
     8401 agaacgaaga cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga
     8461 acttgttgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga
     8521 cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac
     8581 gggtaatgga ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg
     8641 tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac
     8701 tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa
     8761 tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga
     8821 cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaataa acaggatatg
     8881 tgcgaccact cacaaattaa ctttcattac tttccaaggt atcgttatta caattaaata
     8941 cctttaaata tcaacaagtt aaagcataaa aatcagcata aaccctgatt tctatcaaaa
     9001 aaaaatgacg gggataacca tattttatgg cgataacatc attcgttatg tggttaatac
     9061 aaaaaagggg ctgagaccat atttcaagca attaccggat aattaccagg caaaatttga
     9121 gttaaattta atgagcaagt tctcaaattt tattataaat aaaccatttt cagcgataaa
     9181 tactgcggca cgtcacattt tttcacgcta tttgttggag aacaaacatt tattttatca
     9241 atattttaaa atttcgaata caggtattga tcatctcgaa caattgatta acgtcaactt
     9301 tttctcttct gacaggacgt cattttgtga atgcaatcgt tttccataaa ttcttctccc
     9361 ctcataggcg acgaatagca ttttgtgttg aggatcacaa aacgaataat tgctggtcgc
     9421 cgcgataagg tcagacgaag tcaacaaggg ggattttcac tgtgctttac atcagcgtag
     9481 gacacagaat gttgcttcgt ttactgtaac gccgggtaaa tgagcgtttt ttgatagtgc
     9541 gtaagtgtcg tgcgaggaaa ataagcatct attattgttt gtcatgacga caaggcaggc
     9601 atcaatgaaa ggcttcccaa ttgcacatat ttttcaccct tcaatcccgc caatgcacgc
     9661 agtggttaac aatcacaata gaaatattga ttattggacg gtaaaaagaa agtttgcaga
     9721 aattgtctcc accaatgacg ttaataaaat ttacagtata agtaatgaac tgcggagagt
     9781 attatctgca attactgcat tgaatttcta tcaaggcgat gttccttctg tcatgatccg
     9841 aatccaaccg gaaaatatga gtccattcat catagatatt tctacaggag aacatgatga
     9901 ttatatcata caaacattag atgtaggcac ttttgcacct tttggtgaac aatgtacttg
     9961 ctcagccgtc aataaaaaag agctggaatg tattaaggag acgatttcta agtattgcgc
    10021 aaagttcacc caaaaagaag ccatcttaac accccctagg cactttaata aaacctgcat
    10081 tacttcagat tgttggcaaa ttctcttttt ttcacccgat cattttaata atgattttta
    10141 ttgattattt ataaaggata aaataatgtt ccctgtttca tcaattggta atgatattag
    10201 tagtgattta gtccgtcgaa aaatgaatga ccttcctgaa agccccatag gaaataacct
    10261 cgaagcacta gcacccggta tagaaaaact aaaacagacc tctattcaaa tggtcacttt
    10321 acttaacgcg ttacaaccaa gtggaaaatg cattatcact ggtgattttc aaaaagaatt
    10381 agcgtactta caaaatgtaa ttctttataa tgactcgtct cttcgtctgg attttttagg
    10441 ttataacgcc caaattattc aacgatcgga caatacctgc gaacttacca ttaatgaacc
    10501 gttaaaaaac caggaaatat ctacaggcaa tatcaatgtt aattgcccat taaaagatat
    10561 ttacaatgaa atcaggaggt tgaacgtaat ctttagttgt gggactggag atatagttga
    10621 tctatcctct ctggacttac gtaatgtcga tttagattat tatgatttca cagataaaca
    10681 tatggctaat actattctaa atcattttaa attggattca acaaatttta ctaatgccaa
    10741 catgtttcag gttaattttg ttagttcaac acaaaacgcc acaatctcct gggattattt
    10801 actaaaaata acgcctgttt taataagcat tagcgatatg tattctgaag aaaaaatcaa
    10861 gtttgtcgaa agttgtttaa atgagcttgg agacattacc gaagaacaat taaaaattat
    10921 gagatttgca attataaaat ctataccaag ggcaactctt acagataaat tagaaaatga
    10981 attaacaaaa gaaatatata aaagctcatc gaaaatcaac aattgcttga acagaattaa
    11041 attaccagag atgaaagaat tctcatcaaa aaaatatatg attacatcga tacaatcatt
    11101 gaagattatg aaaatatcaa agaaaatgct tatctggtcg tcccccaaat taattatact
    11161 atggatttaa acatagaaga ctctagctca gaagagttaa taccctcgag aaagacgaaa
    11221 attctccgga caatggcttt gaggtcgggg aatataacac atatgaagca tataactcag
    11281 agaatcaata ttttaccaga gaggactata cgtataatta cgacctttta aatgcaatat
    11341 agacttaata acttcttaca aagtctatta ttaataccgt agatattcat cttaccctgt
    11401 gcaccaaaag caaaaactta tcgacgcggg tttgaaactg atggcaaccg caaaaatgcc
    11461 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
    11521 tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa taaagcgcac tcaacaatct
    11581 gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc
    11641 ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
    11701 gccgaagcgg gtttttacgt atatcaggtg aaactgaccg ataagccgct tt
//
LOCUS       DAJTYI010000115        11549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYI010000115 DAJTYI010000000
VERSION     DAJTYI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(6..1061)
                     /gene="dinB"
                     /locus_tag="OO564_003112"
     CDS             complement(6..1061)
                     /gene="dinB"
                     /locus_tag="OO564_003112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6654149.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1132..1917)
                     /gene="lafU"
                     /locus_tag="OO564_003113"
     CDS             complement(1132..1917)
                     /gene="lafU"
                     /locus_tag="OO564_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6654150.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            1889..3601
                     /locus_tag="OO564_003114"
                     /pseudo
     CDS             1889..3601
                     /locus_tag="OO564_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(3818..4315)
                     /locus_tag="OO564_003115"
     CDS             complement(3818..4315)
                     /locus_tag="OO564_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654151.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(4491..5012)
                     /locus_tag="OO564_003116"
                     /pseudo
     CDS             complement(4491..5012)
                     /locus_tag="OO564_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            5396..6136
                     /locus_tag="OO564_003117"
     CDS             5396..6136
                     /locus_tag="OO564_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6654152.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6107..6874)
                     /locus_tag="OO564_003118"
     CDS             complement(6107..6874)
                     /locus_tag="OO564_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6654153.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7080..7658)
                     /gene="lpcA"
                     /locus_tag="OO564_003119"
     CDS             complement(7080..7658)
                     /gene="lpcA"
                     /locus_tag="OO564_003119"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6654154.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            7898..10342
                     /gene="fadE"
                     /locus_tag="OO564_003120"
     CDS             7898..10342
                     /gene="fadE"
                     /locus_tag="OO564_003120"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6654155.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(10385..10831)
                     /gene="ivy"
                     /locus_tag="OO564_003121"
     CDS             complement(10385..10831)
                     /gene="ivy"
                     /locus_tag="OO564_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6654156.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11012..>11549
                     /gene="yafV"
                     /locus_tag="OO564_003122"
     CDS             11012..>11549
                     /gene="yafV"
                     /locus_tag="OO564_003122"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6654157.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTA"
BASE COUNT         2691 a         3050 c         3075 g         2733 t
ORIGIN      
        1 atacatcata atcccagcac cagttgtctt tccatttgcg ggtcaagcaa cgtcacatgc
       61 agccccacca gacgcacacc gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg
      121 gcgattaagt cagctttatt cagccgcggc cagacgtgct cctgggtggt ctgctgaaaa
      181 tcgtcgaact ttaatttcac cccctggcga gcaatcagta aatcaggttt tacctttgcc
      241 agacggcgtt caagttccgg atacagccgc tcgataatcg cttcacattc agaccagtga
      301 tgaatatctt ccgccatcgt gcgttccacg ccgacggatt ttcgtaaccg ttcgctgtta
      361 acgtcgcgct cgtcaatccc ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag
      421 cgtttaagca gaatcaccag atcacacttt tgtacatcac cgcaggtccg cagccccatc
      481 gcttccagtt ttgccgccga gactttgccg acgccgggga ttttttccag tggtaaggtt
      541 tgtaaaaatg ccggaacttc tgccggcgta atcacaaact gaccgttggg tttattcatg
      601 tcggaggcga ttttggcgag aaactttact ggtgccacgc ccgcagatgc cgtcagttgc
      661 agctcgttga agatcgtctg gcggatttcc tgggcgatga gggtcgcaga accgtggcaa
      721 tggacgctat cggtaacatc gagataagcc tcatccagtg acaacggttc aatgcgcgag
      781 gtgtagcgcg agaagatttc acggatatga tttgaggctt ctttgtaggc gtcaaagcgc
      841 cccggaagca aggtgagatg tgggcataat ttgagcgcca tcccagtagg catagcgcta
      901 cgtacgccaa atttacgcgc gggataattg gcggtgctga tcaccccccg acgttcgcgg
      961 ctgccgccaa tagcaatagg gatatcgcgc agagcgggat tgtcgcgcat ctccaccgct
     1021 gcgaaaaagc agtccatatc cacatgaatg attttacgca ttgctcacct ctcaacactg
     1081 gtaaagtata cagtgatttc aggttttgag aaatgcgtaa agattcagca tttagcgcat
     1141 tcgctgcgaa agcacctgct gcttatccag cttttgcacc agcggctgca ccactttatc
     1201 cccatgctga ccaaagtatt gatacagcgt atcggacgca cttttggtca gtaccataat
     1261 ctcaatacgc cgattgcccg cgctttgcgg attcttcgcg tccagcagca tctggtccgc
     1321 cattgcgctt acctgcatca ctttatcttc cggcattccg gcctcttcca gcacccgacg
     1381 agctgaaagc gcgcgatcgc ccgaaaggtt ccagttgttg tagatattgt ttttgtaggc
     1441 catcgcatcg gtatgcccgg taataataat tttgttatag agcgagtcga acactggcgc
     1501 gagctccacc agcaaagttt taaagaacgg cataatctgc gcgctgccgc gttcgaacat
     1561 attgcgattc tggtcgtctt taatcagcac gcgtaatccc tggggaacaa tctccatctc
     1621 cagattcgct tccatatgcg catcacgggc aatggtgttg atactggttg ccaactcgcc
     1681 aagctcggtg gcggatttct tcttcagcag cgccgttttc tcattcacat cacgcgcttt
     1741 tttctccgtc tcttccggca ccgccacggt ggcaggtttt ggcggatgag acgggctgat
     1801 tttatttaac ggcgacagcc caccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat
     1861 aatgctttca cgctcggatt tactgacgct gttgacaatc aactttatcg tcgtcaccaa
     1921 agaggccgag cgtatttccg aggtttctgc ccgctttacc ctggacgcga tgcccggcaa
     1981 acagatggcg attgacgccg atcttaacgc cggattgatc aaccaggcgc aggcgcaaac
     2041 ccggcgtaaa gatgttgcca gcgaggccga tttctacggc gcgatggacg gggcatcgaa
     2101 gtttgtacgc ggagacgcca tcgccgggat gatgattctg gcgatcaacc tgagcggcgg
     2161 cgtctgtatc ggcatcttca aatacaacct gagcgccgac gccgccttcc agcaatacgt
     2221 gctgatgacc atcggcgacg gcctggtggc gcagatccct tccctgctgc tctccaccgc
     2281 ggcggcgatt atcgtcaccc gcgtcagcga caacggcgat atcgcccatg acgtgcgcca
     2341 ccagttgctg gcaagcccgt cggtgctcta caccgccacc gggatcatgt tcgtgctggc
     2401 ggtggtgccg gggatgccgc atctgccgtt tttgctgttc agcgccctgc ttggctttac
     2461 cggctggcgg atgagcaaac gcccgcaggc ggcggaggcg gaagagaaaa gcctcgaaac
     2521 gctgacccgc accatcactg aaaccagcga acaacaggtc agttgggaaa ctattccgct
     2581 gatcgagccc atcagcttaa gcctcggcta caagctggtg gcgctggtgg acaaagcgca
     2641 gggcaacccg ctcacccagc ggattcgcgg cgttcggcag gtgatttccg acggtaacgg
     2701 cgtgctgctg ccggagatcc gcattcggga aaacttccgc ctcaagccca gccagtacgc
     2761 tattttcatc aacggcatta aggctgatga agcggatatt ccggcggata aactgatggc
     2821 gctgccctcc agcgaaacct acggcgagat tgacggcgtg ctggggaacg acccggcgta
     2881 cgggataccg gtcacctgga tccagcctgc gcagaaagcg aaggctctga atatggggta
     2941 tcaggtgatc gacagcgcca gtgtgattgc cacccatgtg aacaagattg tgcgcagcta
     3001 tattcctgat ttgtttaact atgatgacat cacgcagttg cataaccgtt tgtcgtcgat
     3061 ggcaccgcgc ctggcggagg atttaagcgc ggcgctcaat tacagccaat tgctgaaagt
     3121 gtaccgggcg ctgctgaccg aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt
     3181 gctggtcgcc agtagcacgg tgactaaaga tcatattctg ctggcggccg atgtgcgcct
     3241 ggcgctgcgg cgcagcatta ctcatccgtt cgttcgcaag caggagctga cggtgtatac
     3301 gctgaataat gagctggaaa atctgctgac caacgtagtg aatcaggcgc aacaggccgg
     3361 caaagtgatg ctcgacagcg tgccggttga cccgaatatg ctcaaccagt tccagagcac
     3421 gatgccacag gtgaaagagc agatgaaagc ggcggggaaa gatccggtgc tgctggtgcc
     3481 gccgcagcta cgccctttgc tggcgcgtta tgcaaggttg tttgcgccgg ggctgcatgt
     3541 gctgtcgtat aacgaagtgc cggatgagct ggagttgaag attatggggg cgttgatgta
     3601 agaatccggc atcggtgcac aattgccgga tgcggcgtaa acgccttatc cggcctaccg
     3661 ttcaggcaca agtttttagg catgataaga cgcgccagcg tcgcatcagg catctgcgca
     3721 taactgccgg atgcggcgtg aacgccttat ccggcctacc gttaaagcac gatttgtagg
     3781 ccggataagc gtaagcgcat ccggcaccag cgcacagtta taaaatgata cgctccccgg
     3841 cacttaaatc cgttatgtct cccgcccaat cgatgggata taaccctcgc gcgacatcgc
     3901 gatggaacgt tgagaatggc caatcactca cctgctttac ccagccatgt ttctctggat
     3961 ttatataaat ataatcaaca tgatggcgat aatcttttgt attgcgaatg gcatgctccc
     4021 aaaaacgcgg ttgccagata tttttcaatc cgcaagcatg ggtgaactgc tttttaatct
     4081 cccgccagcg agaggaaaaa tcatcatcgc tttcgggtaa tgtccatatg caatgcatat
     4141 gctccggcaa aacgacccag gcgttaattt caaaaggtct gtctcgctta acgttaataa
     4201 tggcgttacg gagcgtctgg aactggatgg tcagaagatg gcttcgacga ttgcgtaaat
     4261 ttaccgtgaa aaaccatgtt ccccccttga tgtaataacg acgatattcc gacatagcga
     4321 ctcctttttc tcaacggtaa gaggaaaagg aaaggccgac aatgacgctg tgttatatcg
     4381 ggaaagcggc tcgcaaaaac gcacagattt gtaggcatga taagacgcgt cagcgtcgca
     4441 tcaggcatcc gtgcacaacc gccggatgcg gcgtaaacgc cttatccggc ctacaaaatc
     4501 atctcttccg tcaaaatcct gcgtgcgcct aaaaaatggt cctgccagaa aggttcggct
     4561 aaccggctta cccgaatggt ttccccggtg cgcagtgact caataaactg accgtcgccc
     4621 agatacaccc ccatatggtc ggcaatctcg cggctgtgga tatggaaaaa tagcaaatct
     4681 ccccggcgca ggtcgttatt cgccacaatc gttgcccgac ggtagtggta catctcatta
     4741 gccgtgcgcg ggagtttcgc ctcaaggatc ttgttgtagg cataaaaaac taacccacta
     4801 caatcgaagc cttgatcggg ccgcgtgccg ccccagacgt aaggcttgcc aagctgctgc
     4861 tccaggcggt gaatcgccac ttcggtgatg ttatgcaggt gatcgctgct taaaaagtgg
     4921 ttgttttccg ccagctcctg ccagcgtctg tcactcgcct tgagcggcgc aggaaaccat
     4981 tctggatgct gttttatctg ctcccggttg tggtggcgct ggcgagtaaa gggagcaaaa
     5041 cgcataacga aaacacgcct ggatgcaaaa atctgccgag caagaacgaa gacatgaaag
     5101 acataaaccg gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat
     5161 taattgctta tttgcatatt actattgaga tttcataata ttaggaaata aatgatacat
     5221 gcgcttttcg gactattgaa aactgcccct gtgctaaatt tgtaaaccca gcgccgacag
     5281 gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact attccgattt
     5341 gggagccatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa gcagtatgcg
     5401 taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg ccggtttgct
     5461 gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt tgatgggatc
     5521 ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctttacg tcaaaatggg
     5581 cgagcaatat caactgctcg acagttataa aatctgtaaa tattccggcg gcttagggcc
     5641 aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc agcgtaatca
     5701 gttaaaacca gacagccgtt actacaaagc gattaatatt ggattcccca atgcttatga
     5761 ccgtgcgcat ggttatgaag ggaaatacct aatgatccac ggcgattgtg tatccatcgg
     5821 ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta ctggtgcgct
     5881 ggtgtttggt cagccgagcg tgcaggtgag tatttaccca ttccgcatga ccgacgccaa
     5941 tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcagctga agccgggcta
     6001 cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggccaatg gccgttacgt
     6061 ggtcagcaag ccgttaagcc acgaagtggt gcagccacaa ctggcatcaa actacacgct
     6121 ccccgaggca aaataagcgc cattcgcctg gcataatttt ttgccaggtt tcattgcccg
     6181 tcagcggctg tgtcgcaatc accgtgacca catcattcgg tgtggtctgc gagctgaagt
     6241 cgatttccac atcctgatcc agcaacgttg ccacgccaaa cggcgcgcgg cgggtgatcc
     6301 agtgtaaatt agtcgagcaa tacgccatta catagcgccc gtccgaaagc agcatgttga
     6361 aaacgccctt cgcccgcagt tcatccgcca gtgaggcgat atatttaaat actgccgcca
     6421 tgttgcccgg cgtgcgcggg taacgctgcg ttaatttatg cagcagccag caaaaggctt
     6481 tttcgctgtc ggtttcaccg accgggcgga agttgccggt ttccagtgat ttgtagcccg
     6541 tcagttgtcc gttatgggca taagtccagt tacgccccca taactcgcgg gtaaatgggt
     6601 gagtattttc cagcgccacc tcgccccgat tagcctggcg aatatgagcc accaccgaac
     6661 aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag ctgggttgtg
     6721 gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag ccatctttat
     6781 gtggcccggt tccaccacca cgctgtacaa gtccggtgaa actaaagcag atatcggtag
     6841 ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac agcgggagcg
     6901 catggacccc ggcgaagatt gatttaagaa ttttcaggtc ggatgcggcg taaacgaatt
     6961 attcggccta cggttcgggt acagatttgt aggcatgata agacgcgtca gcatcgcatc
     7021 aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct acgccagact
     7081 tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt aatgtgaatc
     7141 tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc cgccgtgcca
     7201 gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt ctcacgcgcc
     7261 gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc cagcagtaca
     7321 tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa accgaaatca
     7381 ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta gcccggacgg
     7441 ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca atgggaaccg
     7501 ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa caggaccgcc
     7561 gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag cgtttccgcc
     7621 gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt cagcataaat
     7681 gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg ggcaggtgct
     7741 atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg agctttgttg
     7801 taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca tatcatcaca
     7861 agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga gtattctcgc
     7921 tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta tcagcagtct
     7981 gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcgg cgtgggtact
     8041 ggtgcctctg accattatcc tcgtgccatt taactttgcg cctatgcgta agtcgatgat
     8101 ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa
     8161 agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg gcaagccgga
     8221 ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gctgaagagc aagcgtttct
     8281 cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta ctcatgagct
     8341 ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct tcgcgatgat
     8401 tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc gtgtgctgca
     8461 aaaactctct ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccaa actcattagg
     8521 cccgggcgaa ctgttgcaac attacggcac tgacgagcag aaagatcact atctgccgcg
     8581 tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc agcccggaag cgggttccga
     8641 tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg gccagcaggt
     8701 gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga ttgcgaccgt
     8761 gcttgggctg gcgtttaaac tctccgaccc ggaaaaatta ctcggcggtg cagaagattt
     8821 aggcattacc tgtgcgctga tcccaaccac cacgccgggc gtggaaattg gtcgtcgcca
     8881 cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg tcttcgtgcc
     8941 gatcgattac ataatcggcg ggccgaaaat ggccgggcaa ggctggcgga tgctggtgga
     9001 gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg gcgtgaaatc
     9061 ggtagcgctg gcaacgggcg cgtattctca cattcgccgt cagttcaaaa tctctatcgg
     9121 taagatggaa gggattgaag agccgctggc gcgtattgcc gggaacgctt atgtaatgga
     9181 tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg ccgtgctgtc
     9241 ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg atacgatgga
     9301 tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac ttcctggcgc gtgcttacca
     9361 gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca gcatgatgat
     9421 cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg aagcggcgaa
     9481 gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc acgtcggtag
     9541 caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca gcacgccaac
     9601 cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca acctcgccct
     9661 gctttctgat gtctcgatgg cactgctggg cggcagcctg aaacgtcgtg agcgtatctc
     9721 ggcacgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc tgaagcgtta
     9781 tgacgacgaa ggccgtaatg aagccgatct gccgctggtg cactggggcg tacaggatgc
     9841 gctgtatcag gcagaacagg cgatggacga tttactgcaa aacttcccga accgcgtggt
     9901 tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg caccgtctga
     9961 caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccactc gttcacgtat
    10021 tggtcgcggt cagtacctga cgccaagcga gcataacccg gttggtttac tggaagaggc
    10081 gttggtggat gtgattgccg ccgactcgat tcatcagcgg atctgtaaag agctgggtaa
    10141 aaatctgcca tttacccgtc tggatgaact ggcgcacaac gcgctgacga aggggctgat
    10201 tgataaagat gaagccgcta ttctggtgaa agcggaagaa agccgtctgc gcagtattaa
    10261 cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc cggagaaagt
    10321 gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt tctttatccg
    10381 ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc aacgccgcga
    10441 acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag gtgagtttct
    10501 cttgcgacgt tttctcatca atagccgaga acagccccgt catctgatta gatttctcgg
    10561 accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct
    10621 gatacgtctc atctcccaac gttacggttt gtgcgggagt ataagtaccg cctttcatca
    10681 cccatgcagg tagcttatgc ccttgcacca tctgattaaa cgcagctttg gtggtttcgc
    10741 cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg gcgatgacca
    10801 gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc cgagcttatc ctgcccatgt
    10861 tattaacctc ctaaagcctg gcgataacac gataatttgc aaatcagata tttccaatat
    10921 cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt gttaaagtta
    10981 taaaaccctt cgcataaagg agttgtacac cgtgcctagt ttgaagatta cgcttttgca
    11041 gcaaccgttg gtgtgggtgg atggtcctgc caacctgcgc cattttgatc gtcaactgga
    11101 aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg gctttgccat
    11161 ggaagcggca gcttcgtcgc ttgcacaaga tgacgtagtg aactggatga cagctaaggc
    11221 gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt ctggttcggt
    11281 taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata agcgtcatct
    11341 gttccgcatg gcagatgagc atctgcatta taaagcgggc aatgcgcgag tgattgtgga
    11401 atggcgcggc tggcgtattt tgccgctggt gtgctacgac ttacgttttc cagtgtggtc
    11461 gcgcaatctc aacgattatg acctcgccct gtacgtcgcc aactggccag ctccgcgctc
    11521 tctgcactgg caggcattgc tgacggccc
//
LOCUS       DAJTYI010000116        11538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYI010000116 DAJTYI010000000
VERSION     DAJTYI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..720
                     /locus_tag="OO564_003123"
     CDS             <1..720
                     /locus_tag="OO564_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6654158.1"
                     /translation="PFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVY
                     TGMRHGELVSLAWEDIDMKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVL
                     KNQAEMTRLGKQHHIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSW
                     ETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDS
                     NAEQIAMLNQKLADYVPWSPK"
     gene            733..1503
                     /locus_tag="OO564_003124"
     CDS             733..1503
                     /locus_tag="OO564_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6654159.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1556..1951
                     /locus_tag="OO564_003125"
     CDS             1556..1951
                     /locus_tag="OO564_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6654160.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1992..2735
                     /gene="cmoA"
                     /locus_tag="OO564_003126"
     CDS             1992..2735
                     /gene="cmoA"
                     /locus_tag="OO564_003126"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6654161.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2732..3703
                     /gene="cmoB"
                     /locus_tag="OO564_003127"
     CDS             2732..3703
                     /gene="cmoB"
                     /locus_tag="OO564_003127"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6654162.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3868..6297)
                     /gene="torZ"
                     /locus_tag="OO564_003128"
     CDS             complement(3868..6297)
                     /gene="torZ"
                     /locus_tag="OO564_003128"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6654163.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6322..7422)
                     /locus_tag="OO564_003129"
     CDS             complement(6322..7422)
                     /locus_tag="OO564_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6654164.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7810..8556)
                     /gene="cutC"
                     /locus_tag="OO564_003130"
     CDS             complement(7810..8556)
                     /gene="cutC"
                     /locus_tag="OO564_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6654165.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8570..9136)
                     /locus_tag="OO564_003131"
     CDS             complement(8570..9136)
                     /locus_tag="OO564_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6654166.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9352..11085
                     /gene="argS"
                     /locus_tag="OO564_003132"
     CDS             9352..11085
                     /gene="argS"
                     /locus_tag="OO564_003132"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6654167.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11262..>11538
                     /locus_tag="OO564_003133"
     CDS             11262..>11538
                     /locus_tag="OO564_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR6654168.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLN"
BASE COUNT         2832 a         2951 c         2789 g         2966 t
ORIGIN      
        1 cccttccagg gaattaagcc tcttaaaaga gccagggcag agccagatcc gttaactcgt
       61 gacgaattta ttcgcctgat agatgcttgc cgacatcagc agacgaaaaa cctgtggtca
      121 ttggctgtgt acacaggaat gcgtcacggt gaactggtct ccctggcctg ggaagatatc
      181 gatatgaaag caggaactat tactatcagg cgcaattata cgaaactcgg tgagttcact
      241 ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc cgctatcagt
      301 gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcaccacat caaggttcaa
      361 ctacgtgaat atggacgttc agtgaatcat gaatgtactt tcgtatttaa cccccaggtg
      421 gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg cgactcatgg
      481 gaaacagcca ttaagcgtgc gggcatcagg caccggaaag cataccagtc acgacacact
      541 tatgcgtgct ggtcattatc tgccggagca aacccaagct tcattgccag ccagatgggc
      601 catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga cagtaatgca
      661 gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccatggtc accaaagtga
      721 cacaagagac ttattaaaat cagtgggtta gccttcaata cccgtcatgt taactgcgtg
      781 gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg ctggcgtgaa
      841 cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc gcatcaggca
      901 gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc accgttggct
      961 ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg ggagctgcct
     1021 gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt ggaagtccgc
     1081 catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg tttacatcag
     1141 cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcatcacg tccacacagt
     1201 gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
     1261 gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
     1321 tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
     1381 ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
     1441 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
     1501 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
     1561 aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
     1621 tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg aactgcaaag
     1681 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgctgatgct
     1741 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
     1801 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct ggcgacgttc
     1861 cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
     1921 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
     1981 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
     2041 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
     2101 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
     2161 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca
     2221 acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg atgattgaac
     2281 gctgccgtcg tcatattaac gcctataaag cccctacgcc agtagacgtc attgaaggtg
     2341 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
     2401 tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga ctgaacccgg
     2461 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgt
     2521 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
     2581 agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
     2641 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
     2701 cactggtggc attaaaggca gaggacgctg catgatcgac tttggtaact tttattctct
     2761 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga ttgctaactg
     2821 gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacacggtgg aatttctgcc
     2881 tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa gcgaagagcc
     2941 actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga tgccatggcg
     3001 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
     3061 atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc tggatgtcgg
     3121 ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg
     3181 gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
     3241 cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac tgaaagcctt
     3301 tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg agcatctctg
     3361 gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc tggttattga
     3421 tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc gtaatgtcta
     3481 tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat
     3541 ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg aatggatggt
     3601 taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag ttgaaggtta
     3661 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg gtattactgc
     3721 cggatgcggc gtgaacgcct tatccggcct acaaaccctt gctaattcaa tctattgcag
     3781 ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc ataacctcag
     3841 cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcaggcttat caaatgccgt
     3901 taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt taccggcaca
     3961 ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac tgccgttttt
     4021 acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca cgcctttttt
     4081 gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc agacacgcac
     4141 cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag tgatcggttc
     4201 acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac tgtgtaaacg
     4261 gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac ccttccactc
     4321 atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt tttccagcgt
     4381 tttggagtaa atctcaattt tgccgcttgg cgtacccagc gcatttttca ccggatcggc
     4441 gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca tttcgatcag
     4501 tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg cacgggcccc
     4561 tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac cttcggtata
     4621 gatctctttt ccgccaggtt tgagtaattc cgcaagatcg gcaaacacgt caaaatcgtt
     4681 acgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct ggttgctgta
     4741 atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta atacgatatc
     4801 tgcgtgtttg gctgctgcgg tccagtagca ttcagaaacg acgatcatct ccggtttctg
     4861 ccaggctttg atcagacgat tagtatcctg gtgatgggta aagttaccgc cacccgccca
     4921 ccagatcatt ttgatattcg gatacgtttg ttctttaccg ttatgctgat atttgccgcc
     4981 aggattttcc agcgcatcga cgatacgggc aacaggaatc gccgccattc cgccatcatc
     5041 agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca cgccgccaac
     5101 acgcatcggg ttgccaccat tggagtaatg ataagagaaa ccaaaaccgc cgcccggtgt
     5161 accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct gttcaccata
     5221 ttgctggcgc tgaattcccc agcctgccat cagaattgtg cgattcgccg ccatcagttc
     5281 agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc agaccgcgct
     5341 cttcggcgta ttatcgctct tacctgtcag atactcttca aactgcggat acccggtagt
     5401 gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat gcgcaatccc
     5461 taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat tatcgccaaa
     5521 gaattcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc cagatttttt
     5581 cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa tttttaaggt
     5641 attcagcggg ttcattcccc acagcaccac aacctggctg ttttccagaa tcagcggcca
     5701 ggaggcctgc tgttcataca cctctacaga accgaccaca tgcggcatga tcacctgtgc
     5761 tgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt tcatataacg
     5821 ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt aagatccggc
     5881 aaaaatggcc gatggtccgt tagctttacg aatacgctca tgttgctcat gaatcagttt
     5941 taatgcctgt tcccagctca cctgtacata ggtatcttcg ccacgacctt tcgccggttg
     6001 cagtggattg tcgagatagc tttttctcac catcggatgc tgaatacgcg ccgtggtgtg
     6061 tacctgatcc gccgccgtag actgtaagga gttcggtatg gttttcgcca gcgcgcctgt
     6121 cgaagaaaca atcttgccgt ccttcacttc tacgctcatc gctccccaac gtcccgcggt
     6181 gaggatttta ccgcccttct cttctgccca tgcggggagc ggtgctgccg atgtcaccac
     6241 cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta atgccataac
     6301 ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt tgtactggaa
     6361 ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc ccattccttt
     6421 ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagagg catgacaacc
     6481 agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac ggtccgctaa
     6541 tgcgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc cgtattcgtc
     6601 agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta atgtggctag
     6661 cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca tttgataacc
     6721 ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat cggtaccagg
     6781 attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat gtgcgccatt
     6841 agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat ccatttttat
     6901 ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc cgcccttttg
     6961 tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca tggcatcaaa
     7021 agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc atactgtttc
     7081 ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc cgctaacaaa
     7141 ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca tccctgactt
     7201 tggaatatgg caatcggcac attccgcacg tatccctttc tggttcgaaa agtggacagt
     7261 tccctgatat tcctcaaaag gtttactcat tgagtggcag gaaaggcaaa atgctgtatc
     7321 cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa ccacaaccgc
     7381 tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct ctaaataaat
     7441 tccagaacac cgaaatcatt gtataattaa atacacaacg aattaaccgc ttgcataact
     7501 cggcacaaca ctgcctgaaa taatcgataa agaatatgat ttattacaat gtaatcatta
     7561 attgctaagg aataacccag ttgctattta gaatatatat ttatcagttt tagcaattta
     7621 aatcccataa tcaatgtgaa tataaaaaat atttttagca caacgctatg cgcactgtca
     7681 cccgctcaac agatcacggt tatcgttcgt tttttatact gctcagggat aaaaaaaggc
     7741 ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga catgatgcaa
     7801 cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag caaccgccgc
     7861 cccgtctacg atataacgcg aatactcgtc cgcgtgttca tctgatgaca tggacaatcc
     7921 ttgattacga taacgcatcg gtgaggcttg ccacgctccc gcggagctat ggacttccag
     7981 cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc cagccataat
     8041 gattggagca tcacgatggg caataagttc cataattttt gataaacctt gcagtgcgtc
     8101 tgatttttgc cctgatgtca gtacccgggc aatgcccaat tccgcaagat tattgagtgt
     8161 atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca gcggaccggc
     8221 agcagccatt attttttcca ttcgtggcat atcgacattc ccatcaacat cgagaacgcc
     8281 cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa gaatggcggc
     8341 aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg cgaattatcg gatgcacagg
     8401 gatcgtcacc cgctggcgca cggatttcag tccccccaac gacggcgtta agcccccctc
     8461 ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg ttagtgcaca
     8521 ttccatgctg taacagcaaa tttccagtaa tgccattttt gctccttaat tacgctgact
     8581 gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt gttttgccat
     8641 cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc accttttggt gaactggttt
     8701 taactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc gcacgggcat
     8761 tcagcgtttc tggatcaccc ggcagaacga tttcaatatg ttcccaacct tcgtgtgggt
     8821 aacgtttttc cccaggccac ggtagctcca cattggaaaa ctgccagtgc gcaacctgta
     8881 ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcattttt tctgacaaaa
     8941 gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc ggtgacgttt tgatggcaac
     9001 gcaaagaaat gtgatcggca gtcagcggag tgatatccaa ccccagacgg cgggaaagtt
     9061 catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag
     9121 attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt taccctgttg
     9181 ccctgtgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga aaacggcaat
     9241 acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactcccg ccctaatttc
     9301 tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt
     9361 caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc gcctgcggat
     9421 tgcgaaccgc agattcgtca gtcagcaaaa gttcagttcg gcgactatca ggctaacggc
     9481 atgatggcag ttgctaaaaa actgggtatg gcaccgcgac aattagcaga gcaggtgctg
     9541 actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc aggctttatc
     9601 aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct ggcgtccgat
     9661 cgtctcggtg ttgctacgcc agaaaaacag accattgtgg ttgactactc tgcgccaaac
     9721 gtggcgaaag agatgcacgt cggtcacctg cgctctacca ttattggtga cgcagcagtg
     9781 cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg cgactggggc
     9841 actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa
     9901 atggagctgg ctgaccttga aggtttctac cgcgatgcga aaaagcatta cgacgaagat
     9961 gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat
    10021 ttccgcgaga tgtggcgcaa actggtcgac atcaccatga cgcagaacca aatcacttac
    10081 gatcgtctca acgtgacgct gacccgtgat gacgtgatgg gcgaaagcct ctacaaccca
    10141 atgctgccag gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga aagcgaaggg
    10201 gcgaccgtcg tattccttga tgagtttaaa aacaaggaag gcgaaccgat gggcgtgatc
    10261 attcagaaga aagatggcgg ctatctctac accaccactg atatcgcctg tgcgaaatat
    10321 cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg tcagcatcaa
    10381 cacctgatgc aggcatgggc gatcgtccgt aaagcaggct atgtaccgga atccgtaccg
    10441 ctggaacacc acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt caaaacccgc
    10501 gcgggtggta cagtgaaact ggcagacctg ctggatgaag ccctggagcg tgcacgtcgt
    10561 ctggtggcag aaaagaaccc ggatatgcca gctgacgagc tggaaaaact ggctaacgcg
    10621 gttggtattg gtgcggtgaa atatgcggat ctctccaaaa accgcaccac ggactacatc
    10681 ttcgactggg acaacatgct ggcgtttgag ggtaataccg cgccatacat gcagtatgct
    10741 tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta acgaagagca actggctgca
    10801 gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt
    10861 gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg tgcttacctg
    10921 tacgatctgg ccggtctgtt ctctggcttc tacgagcact gcccgatcct cagcgcagaa
    10981 aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa gacgctgaag
    11041 ctgggtctgg atacgctggg tattgagact gtagagcgta tgtaatcgat ttttcgtgag
    11101 agtgaagcct gatcagggtt agccgatcag gcttttttat tgccatctaa atgtattctg
    11161 aaaacggaca tgccattgtt ttctcactgt tgagtaagag gccagaagcg taatatccag
    11221 gcccaggaaa acgataactg ttcaatttaa ggaataccga agtgtttaaa tttcttgtat
    11281 taacattagg cattatctct tgccaggctt acgcagaaga tacggttata gtaaacgacc
    11341 atgacacttc agccatcaaa gattgttggc aaaaaaattc agatgatgat actgacatta
    11401 acgtgatcaa atcatgcctg cgacaagaat acaatctcgt cgatacgcaa ttaaataaag
    11461 cctatggtga agcttatcgt tatatagaac aagtgccacg cacaggtgca aaaaaacttg
    11521 atactgaaca acttaact
//
LOCUS       DAJTYI010000117        11498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYI010000117 DAJTYI010000000
VERSION     DAJTYI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            82..564
                     /gene="ybiA"
                     /locus_tag="OO564_003134"
     CDS             82..564
                     /gene="ybiA"
                     /locus_tag="OO564_003134"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6654169.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(771..1958)
                     /gene="rhlE"
                     /locus_tag="OO564_003135"
                     /pseudo
     CDS             complement(771..1958)
                     /gene="rhlE"
                     /locus_tag="OO564_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            2187..2858
                     /gene="cecR"
                     /locus_tag="OO564_003136"
     CDS             2187..2858
                     /gene="cecR"
                     /locus_tag="OO564_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6654170.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2861..3856
                     /gene="hlyD"
                     /locus_tag="OO564_003137"
     CDS             2861..3856
                     /gene="hlyD"
                     /locus_tag="OO564_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6654171.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3849..5585
                     /locus_tag="OO564_003138"
     CDS             3849..5585
                     /locus_tag="OO564_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6654172.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5578..6711
                     /locus_tag="OO564_003139"
     CDS             5578..6711
                     /locus_tag="OO564_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6654173.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6722..7828
                     /locus_tag="OO564_003140"
     CDS             6722..7828
                     /locus_tag="OO564_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6654174.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7790..8200)
                     /locus_tag="OO564_003141"
     CDS             complement(7790..8200)
                     /locus_tag="OO564_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6654175.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8333..9094
                     /locus_tag="OO564_003142"
     CDS             8333..9094
                     /locus_tag="OO564_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6654176.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMAAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9091..10332
                     /gene="clsB"
                     /locus_tag="OO564_003143"
     CDS             9091..10332
                     /gene="clsB"
                     /locus_tag="OO564_003143"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6654177.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10332..11288
                     /locus_tag="OO564_003144"
     CDS             10332..11288
                     /locus_tag="OO564_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6654178.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11324..>11498)
                     /locus_tag="OO564_003145"
     CDS             complement(11324..>11498)
                     /locus_tag="OO564_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR6654179.1"
                     /translation="NKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFFGILAAIA
                     ITLVWQRHTRFFH"
BASE COUNT         2646 a         3038 c         3135 g         2679 t
ORIGIN      
        1 ggcatttttc ggttttcagg ggtaatggtt tttctgtttc tggaaagcgg ctcgcaataa
       61 ccaccaacat aatgcggttt aatgcccgtt cgagcacaaa ggatccaaca cgtcatgcaa
      121 gacaccatca taaatttcta cagcaccagc gatgactacg gtgatttttc taactttgcc
      181 gccaggccca tcaaggttga cgggaatacc tggcccacct ccgaacacta ttttcaggca
      241 caaaaattcc ttgatgaaaa ataccgcgaa gagatccgtc gggtttcttc ccccatggtc
      301 gccgctcgca tggggcgtaa tcgttccaag ccacttcgta aaaactggga gtcggttaaa
      361 gaacaggtga tgcgtaaagc acttcgcgct aaattcgaac agcatgcaga actgcgtgtg
      421 ctcttgctgg caaccgcgcc cgcaaaactg gttgagcata cggaaaacga tgcttactgg
      481 ggagacggtg gtaatggtaa gggcaagaat cgactgggct accttttaat ggagttgcgc
      541 gaacaattgg ctatagagaa gtaacgtctc ggtggcttat gggcggtaaa acagataaat
      601 tttttcccgt gttctcaaat tgctgacaag tgcttgttgt gaaatgccgg atgcggcgta
      661 aacgccttat ccggcctaca aaagcatgca aattcaatat attgcagagt ccacgtaggc
      721 ctgataagcg tagcgcatca ggcagttttg cgtttgttca tcagcctgat gccgccacgt
      781 tgctggcgac cgttctggat cggttcggct ttgattgacg gatccggctc atagcccgga
      841 atcgcaatgc gcgggatctc ttttttcagc agtttttcga tatcacgcag cagtttgtgt
      901 tcatcaacac acaccagcga caacgcttca ccggtagcag ccgcacgacc ggtacgcccg
      961 atacggtgga cataatcttc cggtacgttt ggcagttcat agttgacgac gtgcggcaac
     1021 tcttcgatat ccagaccgcg cgcagcgata tcggtcgcca ccagtacgcg aatgtcaccc
     1081 gatttaaaat cagccagcgc acgggtacgc gcaccttgcg atttattccc gtggatcgcc
     1141 acactacgga tgccatcttt attgagctgc tcagccagat ggttagcgcc gtgtttggta
     1201 cgggtaaaca ccagcacctg ctgccagttc cctttaccaa tcatgtgcga cagcaattcg
     1261 cgtttgcgtt tcttatcgac aaagtgaacg tgctgggtca cctgatcaga cgcggtattg
     1321 cggcgtgcca cttcgatttc cagcgggttg tgcaacagtt tttccgccag ggctttaata
     1381 tcgtcagaga aggtcgcaga gaacagcagg ttctggcgtt tcgctggcag ttttgttaac
     1441 acgcgacgga tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc atcgaggacg
     1501 aggatttcaa cctgatccag cttcactgca ttttgatgtt ccaggtccag caaacgtccc
     1561 ggcgttgcca ccagcacatc aacgccgccg cgcaacttca tcatttgtgg gttaatgctg
     1621 acaccgccaa acaccaccag cgaacgaatg ttcaggtatt tgctgtaatc acggacgttt
     1681 tcgccaatct gtgccgccag ttcacgggtc ggggtaagaa tgagcgcacg taccggacga
     1741 cgccctttgg cgtgcggctg gcgagtgatc aggtgttgca acagcggcag cgtaaagccc
     1801 gctgttttgc cggtgccggt ctgggcgcta gccatcaggt cgcggccttc cagcaccgca
     1861 gggatcgcct gctgctgaat aggggtgggt tcacggtaac cctgctcggc aacggcgcgc
     1921 aggatatcag ggcttaaacc caaagaatcg aaagacataa ctactccgaa ccgccccgac
     1981 cgttaccggt gtagttttca gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc
     2041 atgagggagc ggagtgtagc agtttttgtg acgcagcgca taaattatcc ctgtgactgg
     2101 cgtagctaaa attcttagcc atactggaca actcccatat ggagtcataa tcttaatcaa
     2161 tcatttgatt aagaatggac tgtgcgatga ataatcctgc catgacaatc aagggtgaac
     2221 aggcgaaaaa acagctgatt gctgccgcac tggcgcagtt tggtgaatat ggaatgaacg
     2281 ccaccacccg cgagatagcc gcccaggccg ggcagaatat cgctgccatc acctactact
     2341 tcggttcgaa agaagatttg tacctcgcct gcgcccagtg gattgccgat tttattggcg
     2401 agcagttccg tccgcatgcc gaggaagccg aacgcttgtt cgcccaacca cagcctgatc
     2461 gggctgccat ccgtgaactg atccttcgcg cctgcaggaa catgattaag ctgctcaccc
     2521 aggatgacac cgtcaacctc agcaagttta tctcccgtga gcagctctct cccacggcag
     2581 cctaccacct ggtgcacgaa caggtgatta gtccgctaca cagccacctg acacgcctga
     2641 ttgccgcctg gaccggctgc gacgctaatg acacccgcat gatccttcat tcccatgcgt
     2701 tgattggtga gattctggcg ttccgtcttg gcaaagaaac aattctgtta cgtaccggct
     2761 ggaccgcgtt cgatgaagaa aagaccgaac tgatcaacca gacggtgacc tgtcatatcg
     2821 acctgatttt gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat
     2881 cggactggcg gtagtggtac ttgccgccgt ggttgccgga ggctactggt ggtatcaaag
     2941 ccgccaagat aacggcctga cgctgtatgg caacgtggat attcgtacgg tgaatcttag
     3001 tttccgtgtt ggggggcgcg ttgaatcgct ggcggtggac gaaggtgatg ctatcaaagc
     3061 gggccaggtg ctgggcgaac tggatcacaa gccgtatgag attgccctga tgcaggcgaa
     3121 agcgggcgtc tcggtggcac aggcgcagta tgatctgatg cttgccgggt atcgcgatga
     3181 agaaatcgct caggccgccg cagcggtgaa acaggcgcaa gccgcctatg actatgcgca
     3241 gaacttctat aaccgccagc aaggactgtg gaaaagccgc accatttcgg caaatgacct
     3301 ggaaaatgcc cgctcctcgc gcgaccaggc acaggcaacg ctgaaatcag cacaggataa
     3361 attgcgtcag taccgttccg gtaaccgtga acaggatatc gctcaggcga aagccagcct
     3421 cgaacaggcg caggcgcaac tggcgcaggc ggagctgaat ttacaggact caacgttgat
     3481 agcaccgtct gatggcacgc tgttaacgcg cgcggtagag ccaggcacgg ttctcaatga
     3541 aggtggcacg gtgtttaccg tttcactaac gcgtccggtg tgggtgcgcg cttatgttga
     3601 tgaacggaat cttgaccagg cccagccggg gcgcaaagtg ctgctttata ccgatggtcg
     3661 cccggacaag ccgtatcacg ggcagattgg tttcgtttcg ccgactgctg aatttacccc
     3721 gaaaaccgtc gaaacaccgg atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt
     3781 gaccgacgcc gatgatgcgt tacgccaggg aatgccagtg acggtacaat tcggtgacga
     3841 ggcaggacat gaatgatgcc gttatcacgc tgaacggcct ggaaaaacgc tttccgggca
     3901 tggacaagcc cgccgtcgcg ccgctcgatt gtaccattca cgccggttat gtgacggggt
     3961 tggtggggcc ggacggtgca ggtaaaacca cgctgatgcg gatgttggcg ggattactga
     4021 aacccgacag cggcagtgcc acggtgattg gctttgatcc gatcaaaaac gacggcgcgc
     4081 tgcacgccgt gctcggctat atgccgcaga aatttggtct gtatgaagat ctcacggtga
     4141 tggagaacct caatctgtac gcggatttgc gcagcgtcac cggcgaggca cgtaagcaaa
     4201 cttttgctcg cctgctggag tttacgtctc ttgggccgtt taccggacgc ctggcgggca
     4261 agctctccgg tgggatgaaa caaaaactcg gcctggcctg taccctggtg ggcgaaccga
     4321 aagtgttgct gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt
     4381 ggcagatggt gcatgagctg gcgggcgaag ggatgttaat cctctggagt acctcgtatc
     4441 tcgacgaagc cgagcagtgc cgtgacgtgt tactgatgaa cgaaggcgag ttgctgtatc
     4501 agggagaacc aaaagccctg acacaaacca tggccggacg cagctttctg atgaccagtc
     4561 cacacgaggg caaccgcaaa ctgttgcaac gcgccttgaa actgccgcag gtcagcgacg
     4621 gcatgattca ggggaaatcg gtacgtctga tcctcaaaaa agaggccaca ccagacgata
     4681 ttcgccatgc cgacgggatg ccggaaatca acatcaacga aactacgccg cgttttgaag
     4741 atgcgtttat tgatttgctg ggcggtgccg gaacctcaga atcgccgctg ggtgcaatat
     4801 tgcatacggt agaaggcaca cccggcgaga cggtgatcga agcgaaagaa ctgaccaaga
     4861 aatttggtga ttttgccgcc accgatcacg tcaactttgc cgttaaacgt ggggagattt
     4921 ttggtttgct ggggccaaac ggcgcgggta aatcgaccac ctttaagatg atgtgcggtt
     4981 tgctggtgcc gacttccggc caggcgctgg tgctggggat ggatctgaaa gagagttccg
     5041 gtaaagcgcg ccagcatctc ggctatatgg cgcaaaaatt ttcgctctac ggcaacctga
     5101 cggtcgaaca gaatttacgc tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga
     5161 acgaaaaaat ctcccgtatg agcgaggcat ttggcctgaa aagtattgcc tcacacgcca
     5221 ccgatgaact gccattaggt tttaaacagc ggctggcgct ggcctgttcg ctgatgcatg
     5281 aaccggatat tctctttctc gacgaaccga cttccggcgt tgaccccctc acccgccgtg
     5341 aattttggct gcacatcaac agcatggtag agaaaggcgt cacggtgatg gtcaccaccc
     5401 actttatgga tgaagcggaa tattgcgacc gcatcggcct ggtgtaccgt gggaaattaa
     5461 tcgccagcgg tacgccggac gatttgaaag cacagtcagc taacgatgag caacccgatc
     5521 ccaccatgga gcaagccttt attcagttga tccacgactg ggataaggag catagcaatg
     5581 agtaacccga tcctttcctg gcatcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag
     5641 atcgttcgcg atccgagtag ctggctgatt gcggtagtga tcccactgct actgctgttt
     5701 atttttggtt acggcattaa cctcgactcc agcaagctgc gggtcgggat tttgctggaa
     5761 cagcgtagcg aagcggcgct ggatttcacc cacaccatga ccggttcgcc ctacatcgac
     5821 gccaccatca gcgataaccg tcaggaactg atcgccaaaa tgcagtcggg gaaaattcgc
     5881 ggtctggtgg ttattcccgt ggatttcgcc gaacagatgg agcgcgccaa cgccaccgca
     5941 ccgattcagg tgatcaccga cggcagtgag ccgaataccg ctaactttgt gcaggggtat
     6001 gtcgaaggga tctggcagat ctggcaaatg cagcgagcgg aggacaacgg gcagactttt
     6061 gaaccgctta ttgatgtaca aacccgctac tggtttaacc cggcggcgat aagccagcac
     6121 tttattatcc cgggtgcggt gaccattatc atgacggtca tcggcgcgat tctcacctcg
     6181 ctggtggtgg cgcgagaatg ggaacgcggc accatggagg ctctgctctc tacggagatt
     6241 acccgcacgg aactgctgct gtgtaagctg atcccttatt actttctcgg gatgctggcg
     6301 atgttgctgt gtatgctggt gtcagtgttt attctcggcg tgccgtatcg cggttcgctg
     6361 ctgattctgt tttttatctc cagcctgttt ttactcagta ccctgggaat ggggctgctg
     6421 atttccacga ttacccgcaa ccagttcaat gccgctcagg tcgccctgaa cgccgctttt
     6481 ctgccgtcga ttatgctttc cggctttatt tttcagatcg acagtatgcc cgcggtgatc
     6541 cgcgcggtga cgtacattat tcccgctcgt tatttcgtca gcaccctgca aagcctgttc
     6601 ctcgccggga atattccagt ggtgctggtg gtaaacgtgc tgtttttgat cgcttcggcg
     6661 gtgatgttta tcggcctgac gtggctgaaa accaaacgtc ggctggatta gggagaagag
     6721 catgtttcat cgcttatgga cgttaatccg caaagagttg cagtcgttgc tgcgcgaacc
     6781 acaaacccgc gcgattctga ttttacccgt gctaattcag gtgatcctgt tcccgttcgc
     6841 cgccacgctg gaagtgacta acgccaccat cgccatctac gatgaagata acggcgagca
     6901 ttcggtggaa cttactcaac gttttgcccg cgccagcgcc tttacacatg tgctgctgct
     6961 gaaaagccca caggagatcc gcccgaccat cgacacacaa aaggcgttac tactggtgcg
     7021 tttcccggct gacttctcgc gcaaactgga taccttccag accgcgccgt tgcagttgat
     7081 cctcgacggg cgtaactcca acagtgcgca aattgccgcc aactacctgc aacagatcgt
     7141 caaaaattat cagcaggagc tgctggaagg aaaaccgaaa cccaacaaca gcgagctggt
     7201 ggtacgcaac tggtataacc cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat
     7261 cgccatgatc accactatcg gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg
     7321 tgaacaaggt acgctcgatc agctactggt ttcgccgctc accacctggc agatcttcat
     7381 cggcaaagcc gtaccggcgt taattgtcgc caccttccag gccaccattg tgctggcgat
     7441 tggtatctgg gcgtatcaaa tccccttcgc cggatcgcta gcgctgttct actttacgat
     7501 ggtgatttac ggtttatcgc tggtgggatt cggtctgttg atttcatcac tctgctcgac
     7561 acaacagcag gcgtttatcg gcgtgtttgt ttttatgatg cccgccattc tcctttccgg
     7621 ttacgtttcg ccggtggaaa acatgccagt gtggctgcaa aatctgacgt ggattaaccc
     7681 tattcgccac tttacggaca ttaccaagca gatttatttg aaggatgcga gtctggatat
     7741 tgtgtggaat agtttgtggc cgctactggt gataacggcc acgacagggt cagcggcgta
     7801 cgcgatgttt agacgtaagg tgatgtaact tcttatcttt cgccagcaaa gacactaccg
     7861 ccgggccagc aaggatcgcc agccctgcaa ttgccagcaa aaagttgttt tccagtacgc
     7921 gggacaacag ccacagcaca atcagcgctg cgccaaaata ccaggtcgtg gtcagctctt
     7981 ccagcacgtc tgccacctca cgccagcgtt gctcggggtg gcgctgaaac accagcatca
     8041 tcactaccgt gacacaatac agtgcgcata atcccacgcc gacgtgaacc agagtcttac
     8101 tcatccagcc caccaccagc agcgccacta ccagtaaatg caacataatc tgccagcaac
     8161 ttagacccgt tgcgacacga acacgttgtt gccacttcat ggcttctctc ctgaagaatt
     8221 tatctctact tactcagagt accgaacttt acggaaaatt ccgtaagacc gcaccttttt
     8281 gtgacgccga ctacactatt tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga
     8341 tcaaacacaa caattttcgt tcaaggtgct caccatcaac attcacaaag gctttaccgc
     8401 gtttaaccga cgcttcattt tgccggaact tcgcgatgcc gtgcgtaccg tcagcgccga
     8461 tattgtttgc ctgcaggaag tgatggccgc gcacgaagtt catccgctgc atgtggaaaa
     8521 ctggcccgat acctcgcact acgagtttct cgccgacact atgtggagcg attttgccta
     8581 cggtcgcaat gccgtatacc cggaagggca tcacggcaac gccgtactgt cgcgttatcc
     8641 cattgaacat tatgagaatc gcgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct
     8701 ctactgccgc attgtgccgc cgatgaccgg aaaagcgatt catgtgatgt gcgtacatct
     8761 gggcctgcgt gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga
     8821 gctaccggac ggcgaaccgg tattggtggc gggtgatttc aatgactggc ggcaaaaagc
     8881 taatcatccg ttaaaagtgc aggccggact ggatgagatt tttacccgcg cccacggacg
     8941 cccggcgcgc acgtttccgg tgcaatttcc tctactacga ctggacagga tctacgtcaa
     9001 aaatgccagc gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac acctttctga
     9061 tcatgcccct ttaagtgcgg agattcattt atgaaatgta gctggcgcga aggcaataag
     9121 atccagttgc tggaaaacgg cgagcaatat tatcccgcgg tgtttaaggc gattggcgag
     9181 gcacaagaac gcatcattct tgaaacgttt atctggtttg aggataacgt cggcaaacag
     9241 ctgcatgcgg cactactggc agcagcgcaa cgcggggtta aagcggaagt cttgctggat
     9301 ggctacggtt cgccggatct cagcgatgag tttgtcaatg aactgacggc agctggcgta
     9361 gtgttccgct actacgatcc ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc
     9421 cggatgcatc gcaaaattgt ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat
     9481 tactccgccg agcatatgtc cagctacggt ccagaggcta aacaggatta cgcggtacgc
     9541 cttgaagggc cgattgtcga agatattctc cagtttgagc tggaaaacct gcctggacag
     9601 agcgcggcac gacgctggtg gcgacgtcat cacaaagcgg aagagaaccg ccagccggga
     9661 gaagcgcagg tattgctggt ctggcgcgat aacgaagaac atcgcgatga tattgaacgc
     9721 cattatctga aaatgctcac tcaggcgcgg cgggaagtga ttatcgccaa cgcctacttc
     9781 ttccccggct atcgattttt acacgccttg cgtaaagcgg cacggcgcgg ggtgcggatc
     9841 aaactgatca ttcagggcga accggatatg ccgattgtca gagtcggtgc gcgcttgctg
     9901 tataactatc tggttaaagg cggcgttcag gtttttgagt accgccgccg cccgctccac
     9961 ggcaaagtgg cattgatgga cgatcactgg gcgacagtag ggtccagtaa tctcgatccg
    10021 ctcagtttgt cactgaatct cgaagcaaat gtcatcatcc acgatcgtca ttttaaccag
    10081 acgctgcgcg ataatctgaa cggcattatt gccgcagatt gtcagcaggt ggatgaaacc
    10141 atgctgccca aacgcacctg gtggaacctg accaaaagcg tgctggcgtt ccacttttta
    10201 cgccacttcc cggcgctggt tggctggctt ccggcacaca caccacgtct gacgcaggtt
    10261 gatccccccg cacaaccgac aatggaaacg caggatcggg tagaaactga aaacacgggg
    10321 gtaaaaccct gatgagtaaa tcacacccgc gctggcgctt agcaaagaag atcctcacct
    10381 ggctgttttt tatcgcggtg attgtgttgc tggtggtcta cgccaaaaaa gtggactggg
    10441 aagaggtctg gaaggtcatc cgcgactaca atcgcgttgc gctgcttagt gcggtcgggc
    10501 tggtggtcgt cagctatctg atttacggct gctatgacct gctcgcccgc ttttactgcg
    10561 gtcacaaact ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac gccttcaacc
    10621 tgacgctcag tacctgggtc ggcggcattg gtatgcgcta tcgtttgtac tcccgactag
    10681 ggttaccggg cagcactatt acgcggattt cctcgctcag tattaccacc aactggctgg
    10741 gctatatttt actggcaggg attatcttta ccgcaggcgt ggtggagttg ccggatcact
    10801 ggtatgtcga tcaaactacg ctgcgtattc tcggcattgg cttactgatg attatcgcgg
    10861 tttatttgtg gttttgcgct ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc
    10921 tggtgctgcc ttcatggaaa ttcgccctcg ctcagatgct gatttccagc gttaactgga
    10981 tggtaatggg ggcgattatc tggctgttac ttggtcaaag cgtgaactat ttctttgtac
    11041 tgggcgtgtt actggttagt agtattgctg gcgtcatcgt gcatattccg gcggggatcg
    11101 gtgtgctgga agcggtgttt atcgcactac tggctgggga gcatacctcg aaagggagca
    11161 ttatcgccgc cctgctcgct tatcgcgtcc tgtattactt tatcccgctg ctgctggcgc
    11221 tgatctgtta cctgctactg gaaagccaag cgaagaagct acgggcgaaa aatgaagcgg
    11281 cgatgtgaag tttagttaag ttctttagta tctgcattta cgactaatga aaaaaacgcg
    11341 tatgcctttg ccagacaagc gttatagcta tcgcggcaag tatcccaaag aagccgataa
    11401 aatagtaata gagtgttaat gcacctgtaa aagcgcaacg aatcggactc cacaactctt
    11461 cactatggca ttttcctgcc agttcaagga gcttattt
//
LOCUS       DAJTYI010000118        11381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYI010000118 DAJTYI010000000
VERSION     DAJTYI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..2031)
                     /locus_tag="OO564_003146"
                     /pseudo
     CDS             complement(1..2031)
                     /locus_tag="OO564_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2055..2678)
                     /locus_tag="OO564_003147"
     CDS             complement(2055..2678)
                     /locus_tag="OO564_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6654180.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPSEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2675..4363)
                     /locus_tag="OO564_003148"
     CDS             complement(2675..4363)
                     /locus_tag="OO564_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6654181.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4512..7139)
                     /gene="gyrA"
                     /locus_tag="OO564_003149"
     CDS             complement(4512..7139)
                     /gene="gyrA"
                     /locus_tag="OO564_003149"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6654182.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7286..8008
                     /locus_tag="OO564_003150"
     CDS             7286..8008
                     /locus_tag="OO564_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6654183.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8136..11381)
                     /gene="yfaL"
                     /locus_tag="OO564_003151"
                     /pseudo
     CDS             complement(8136..11381)
                     /gene="yfaL"
                     /locus_tag="OO564_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2615 a         3184 c         2817 g         2765 t
ORIGIN      
        1 tgccggatgc gaaagcagcg actgctgttc cacgcgatcg cgttccagcg tcaataacgc
       61 ttcatcaatt ggctccggga aagtaatcag catcttcgcg gtttcgcctg gctggtacag
      121 cgttttatcc gccacgatat ctaccgtacc agtatgcgcc gtgctgccct taccgctgac
      181 ggcatgactt aacccagcga gaattaagcc gtctttatcg cgtaatgtca gattgtagtt
      241 gccaggttta gcgaaattga cggtaaagga tttgccgcct gacggtagct ctccgctatg
      301 gctcgtgcgg tcttcgagac gcaaccattc atacgtaaca ggaacctgtt ttgaagattc
      361 cagcgcggca taacggaaca caaccgactc gccgctatta ctgtattgtg cggcggtact
      421 taaagagtaa tgcgccaggc cgcgttcaat gaggagctct ttggtggtgg tgacgcgata
      481 cgccgcgccg tcactggcgg agacggttaa caaatagcgg ctcggtttat cggcggcggg
      541 gagatttaac gccacataac cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac
      601 ggggaaacgt ccggcataac gcaaatcgtt accgaccatt gataattgct gagcgcgcaa
      661 acttaactgc acgcgggcat tttttaccgg ctcgccatcc gggtagagca gttgcagttt
      721 gccgctgacc gcttcgccag ttttgaactc ttttttgtcg agagctaaac caatctcgaa
      781 atgtggcttg atgtagtttg ccacgcgaaa actgctgcta tagacctgat tgcggtaagc
      841 aagacgtaac tcataacctc cggcaacggc attttctggc aggcggaaac ttccctgccc
      901 gccattgcgc gcatccagcg tgacatcgac ggtttgcaac agactgccgt tagcgtccag
      961 caccgaaagc ttcgccgggg cgctgacgat gggggatgaa tgcaacggat cgcggaactc
     1021 gcggcccatc actttaacat cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa
     1081 aatatacaag cgggtgttgt agatttcgct ttcgtagaag aagttctcgg agacaaaaac
     1141 gccgccttca gcatccttac ccagaatgta tgaacgttct ggcgatatat gttgtaactg
     1201 caaagtaccg ctgtcatcgg tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa
     1261 gatctctgag ccgggcttcg cttcaccctg ttttttaccc gcggtccata ccagaagctc
     1321 gttacctgac actttgctaa gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg
     1381 atacccacct accatcgcct cgacgaggta cagccccggc tcttgttggc cgagaggaat
     1441 ataaatatta cccggctgcg gcgagatgaa attgctggat gcgccttcca gttttatccc
     1501 ttgctgcggc tcgaccggtt tagcctgcca tagtggataa cggaactgtt ccaccaaggg
     1561 atattttttc agcggggaaa actggttgtt ctgtacataa cgggaaggtt taataatggc
     1621 attgccgaga tgtaattcgg gtaatgcctg agtcacattc tgccgtgact gagaagagaa
     1681 agtacgctgc atcacgcggc gagatttgcc gtaccagtta tcccacagct aggtcagcgt
     1741 attgttcagc ccgtcgccca gatattgcgg ttgcaccaca atgcgatgca ggtttttctg
     1801 ctggcgcaaa aatgccatcg ggtcaggaat acgatacagg cgaacgtcca cgccgccgta
     1861 ctcttccatc tgataacgcc gataatcacg ccccggcgct tccagtcgca ctttcgcctc
     1921 ttcactgctg ctaaaactgc tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc
     1981 atagttactg gaaggaagcg aatcatcagc tttagcaagc cctgttccca caagagacag
     2041 ggacaagcac gctgctatcg cgccagaaaa tttaaacgat aaatgccaat gaaattggga
     2101 ttggaatcgt tgggtatcca tcgggtgtcc ttccatgtca taagttgttg cagactgact
     2161 gcgcgcattc cgttgtcagt tttcgtggcg cttccggtgt ggttgatgac gtaacgcccc
     2221 atccagacca ttaagtgctg ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc
     2281 gcctggttta tgtcttggcc aataaactgg ctgttgtact gaatcaaatt aatcgcggtc
     2341 acgtaggggc cgtttttccc gctcccctga ttccagtttt gcgccagttg acgctgttca
     2401 ggtgttagcg tcatctctga cggcaaatac tggctagata aaccgttact ttttagccat
     2461 ttactgtcgt gaactttcag cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc
     2521 tgctgatacc agcgtggact tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac
     2581 caggcgcgaa acagcctgga ttgttcgaca ttcagcattt cgctatgggc aacaacacaa
     2641 cacagccagc aaatcagcgc cagcagcccg tgtctcatag cggctgccag gttatgggta
     2701 gccactgcca ggcgacaccg ggttccatct gggccagctt catgacataa cgcggttgtt
     2761 gagataaagc gtccagcttc ggcattaata aagtttgtgc ggcgttataa aaaaccggtt
     2821 cgagattctt cggcagactg gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg
     2881 gattgatata gagcggaacg atgccatcag tgggtattac atccaccatt gccgggcggg
     2941 ttttattcag tgtttgcagg gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg
     3001 ttttgttttg catcgccagc gacacacgga aaaaataatc cgacattaat tgatcgggtt
     3061 gcgccgcctg cgctttcgga tactgaccgt atcgggaact cacttcgcga caccagattt
     3121 gcgcttcgcc ctgctgagtc tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt
     3181 gcgcaccaat attttgcgta aacagtttcc ctggcaattg cgcctgttcg gcagtgccat
     3241 caaactgacc gacaaacagc ggggtttgca attttgagtc ttcataccag cacagcccca
     3301 cggcaccgtc taacgcccca ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct
     3361 cttcggcaat accgtgtgaa tacggcaccg ccacacagaa gctggctccg gcaggcatac
     3421 tgttccagac cggcgtaaaa tcgaaactgg catctacgct ggcggattca tcatttaacg
     3481 ccacaaaact gtgccagccg tcgttaccca tttcgaagcg tacgccagca aaagaaggca
     3541 ttaaccgctg gtagccaaaa cccagcaacc tggcgctggc cacgatgcgc aggcgtactg
     3601 gcgttttttc ggctgtacgc tcttccaggc caaagctggc ttgccagcgt tttttaccgc
     3661 tcaacaaatc acttgcgatc gccgtggctt cggtatcctg ctgatcatct ttaaacaaca
     3721 tatccgtact ggaaaacacc agcatcttgt cctgatatgt cgcgaacatc agggcgttat
     3781 tgccgttata gcgcaactga taaacaggaa tagtttcact atttatcttg atgctactga
     3841 tttccgtttt gcttaactgg ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt
     3901 tgcttaaacc actgcgctgg atcagcacca tgtaatgtga aagatggcct tgtttatcgt
     3961 gccatagcgc cgcctgcgcg ggctgatcta agagtgacga aaagagctta tctttcagcg
     4021 tgagatcgtg ttcatagaca atgcggcgaa tgctgccttc aatgcccaga cgatctgcat
     4081 gattctgata atagaaaacg aaatcctcgc tcagaacatc gtggagaaac ggaatggtga
     4141 ggagatcttt gggaagctgg ctcagagagt cgctgtctag aaagaggtcc ggctcattga
     4201 gatcgatttg cagattgttg tgcaccacca gcggcgacaa cgttttttct ggcccactgc
     4261 cagcatattg caacgcccag acgccagcgg aaagcagtgc tattgcgcca aaacctacaa
     4321 gaccatagaa ccgccagcct ttcgcctttt tttcaccact cattgccaca ttccttgtgt
     4381 atagccagcc attttttacg ggcacagcca aactttaccg cgccctaata cgacaaaagc
     4441 ccagactttg cagcctggac ttttcaattc aaacaaggga gatagctccc ttttggcatg
     4501 aagaagtaaa attattcttc ttctggctcg tcgtcaacgt ccacttccgg agcaatttca
     4561 tcatcccctt ccgcggcact gccgtcgatg gtatccagat cttcctcgtc aaccggttca
     4621 gcaacacgct gcagacccac tacgttttca tcttccgcag tacggatgag gatcacgccc
     4681 tgggtgttac ggcccacgat gctgatttct gaaacgcgag tacgtaccag cgtaccggca
     4741 tcggtgatca tcatgatctg gtcgcagtca tctacctgca ccgcgccaac cactaaaccg
     4801 ttacgttcgg taaccttgat ggagataacc cctttcgtcg cacgcgactt ggttgggtat
     4861 tccgccactg cagtacgttt accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg
     4921 ccacgaggca cgatcagaga gacgacttta tcgccttcac ctaagcgaat accgcgcaca
     4981 ccagtggtat tgcagcccat cgcacggaca gaagactctt taaagcgcac cactttacct
     5041 tcggcggaga acagcattac ttcatcttcg ccactggtca gatcaacgcc gatcagctca
     5101 tcgccgtcaa ccagtttgat cgccacttta ccggcggtac gcagacggtt gaattcggtg
     5161 aggacggttt tcttcacagt accgttagcg gtcgccatga agactttcac gccttcttca
     5221 aactcggtca ccggcaggat cgcggtgata cgttcgtcct gttccagcgg tagcaggttg
     5281 acgatcggac gaccgcgcgc gccacgagtg gcttccggca actggtaaac tttcatcgaa
     5341 tagacgcgac cacggctgga gaagcacaga atatggtcgt gcgtgttcgc caccagcagt
     5401 cggtcgataa agtcttcttc tttaatacgt gcggcagatt tacctttccc gccacgacgc
     5461 tgcgcttcgt attcagaaag cggctgatat ttaacgtagc cctggtgaga gagcgtcacg
     5521 accacatctt cctgggtgat cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt
     5581 tcagtacgac gtttgtcacc gaactgttca cgaaccagct ccagctcttc gcggatcact
     5641 tccatcagac gatcggcgct accaagaata cgcaacagtt ccgcgatctg atccagcagc
     5701 tctttgtatt cgtcgagcag tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga
     5761 tccagaatcg cctgagcttg ctgttcggtc aggtagtaca gaccatcacg cacgccgaac
     5821 tctggctcca gccattccgg acgcgcagca tcgtcgccag cacgttccaa catggcggca
     5881 acgttgccca gctgccacgg attagcaacc agcgcagttt tcgcttctgc aggcgtcggc
     5941 gcatgacgga tcagttcgat gatcgggtca atgttcgcca gcgccacggc taatgcttca
     6001 aggatatgag cacgatcgcg agctttacgc agttcgaaaa tagtacgacg ggtcaccact
     6061 tcacggcggt gacgaacaaa cgccgcgatg atgtctttca ggttcatgat cttcggctga
     6121 ccatggtgca atgccaccat gttgataccg aaagaaacct gcaactgggt ctgggagtag
     6181 aggttgttga gcacaacttc accgaccgca tcgcgtttca cttcaatcac gatgcgcata
     6241 ccgtctttgt cagactcgtc acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc
     6301 agttccgcaa tcttctcgat caggcgcgct ttgtttacct gatacggaat ttcgtggacg
     6361 ataatggttt cacgtccggt tttggcgtca acttccactt cagcacgggc gcggatatat
     6421 accttgccgc gaccggtacg gtaagcttct tcaataccgc gacgaccgtt aatgattgcc
     6481 gccgtcggga agtccggccc cgggatgtgt tccatcagcc cttcaatgct gatgtcttca
     6541 tcatcgatat acgccagaca accgttgatg acttccgtca ggttgtgcgg cgggatgttg
     6601 gttgccatac ctacggcgat accggaagaa ccgttcacca gcaggttagg aattttggtt
     6661 ggcatgacgt cgggaatttt ttccgtgccg tcatagttat caacgaaatc gaccgtctct
     6721 ttttcgagat cggccatcag ttcatgggca attttcgcca gacggatttc cgtataacgc
     6781 attgccgccg cagagtcgcc gtcgatggaa ccgaagttac cctgaccgtc taccagcatg
     6841 taacgcagcg agaatggctg cgccatacgg acgatcgtgc cataaaccgc cgagtcacca
     6901 tggggatggt atttaccgat tacgtcacca acgacacggg cagatttttt ataggctttg
     6961 ttccagtcat tgcctagtac gttcatggcg taaagtacgc gacggtgtac cggcttcagg
     7021 ccatctcgga catctggcag cgcacggcca acaatgaccg acatcgcata atccagatag
     7081 gagctcttta gctcttcctc aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc
     7141 taaccgctat ccctctactg tatcccggat tcaaaggtcg caaattataa cacagccgcg
     7201 cagtttgagg taaacctata cgctttattc acatccaatg cctgatatac tcgtttgtct
     7261 tgccaattac ggagtagaag tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt
     7321 agaccatgaa gagatcgcta aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg
     7381 tgagttcaaa ccgttgcacc gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc
     7441 tggcggttta tttggcaaaa aggtgctcga tgtcggttgt ggcggcggca ttctggccga
     7501 gagtatggcg cgcgaaggcg cgacggtgac cggtctggat atgggctttg agccattgca
     7561 ggtggcaaag ctgcacgcgc tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt
     7621 ggaagagcac gcggcaaaac atgccgggca gtatgatgtg gtgacctgca tggagatgct
     7681 ggagcacgta cccgatccgc agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg
     7741 cggcgatgtc tttttctcga cgcttaaccg caacggcaag tcatggctga tggcggtggt
     7801 tggcgcggaa tatattttgc gcatggtgcc caaaggtacg catgatgtga agaagtttat
     7861 taaaccggca gagctgcttg gctgggtgga tcagaccagt ttaaaagagc gacatataac
     7921 cgggctacat tacaacccga tcactaattc ctttaagctc ggccccggcg tggatgtgaa
     7981 ctatatgctg cacacgcaga ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc
     8041 gacgcttgca gcgtcttatc aggcctacaa atgctctccg taggccagat aaggcgttta
     8101 cgccgcatcc ggcaattgtg atggtcagac agcgattacc atttcaccgt catcgacaaa
     8161 aaccctgccg tctgggcaaa atcatcactc cctttctgcc acgccacgct gccgcgcaga
     8221 gacactcgct gactgatatt gcccgtgact cccactttta tttcaccccg tagcttcacc
     8281 gcatcgtcac tgatagtgct gccatcttct tcaatttccg tcgcatgggg atcgtgataa
     8341 taattcagat ctaatgttgg tgtgacacca accgcggtac gccattcgct gtgtaaaccc
     8401 agccgcgtct gaatatcatc cccctgcgat tgtgacacgc gcgcatggtt agcggcggta
     8461 aaatcatcct gctgcacgcc ctgataaatc acctgcgcct gcggttcaat caccacacca
     8521 cgccccggta accactgata cccggcttcc agcgaggcga taatccccga cgaatggtaa
     8581 cgatccacgc catcttcatg ttcggaaaca tcattgttaa accacgcgta ttgcagccag
     8641 ctatccagcc aggccccctg cttctgatta ccgtgctgat accagcttga ggtcagccca
     8701 acggcataac cgtggttctg gttatcggcg cgagttccgg tcatattcga gcggctgtcg
     8761 ccctgattat cactgtagcc accaaccgcc ccaagcatcc actcgccatc cgtgccccag
     8821 cacccgctaa acaagccgcc gctaagcagc accgtagaag tgtcttcctg ctgagccagt
     8881 tgccctgccg ccgtgtaatg ataacgtccg ccgataacac gtaaattcag cgtctgacca
     8941 tcgccaccag cgtgatcgcg tcgctccatc ataaacgcct gatttgccgc ccgcaggtta
     9001 ttgagataac cgccaacttt ggcattcagc accggctggt aagcaggcgt aggggccggg
     9061 tcgggggttg ggtcaggatc cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt
     9121 ggcgatggcg gcgttacttc ttgcgatcgc agataccagt cgttgttatc ttccactagc
     9181 gtgtagtcat acgctcccat gttgacgtag ccactgccag ccagactgaa ctgtgcattg
     9241 ttttgaaact gcgtgggatc agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc
     9301 tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt tgccagcagt attaccgttc
     9361 atcaccaatt gatcgcttac cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta
     9421 ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg
     9481 ctaaccgcac cggacgtatt ggtcagatca ccattaaccg tcacgtcagc aagataggtc
     9541 aggctgtcag tttcaaggtt ggtattaagc tcgcctgtca acgtcacgcc atcggtagca
     9601 tttaccagcg tcccatcgcc gttaaatagt gaggcattaa gggcgacgga agtatcctgc
     9661 ccggtcagac gcaaaaccgt accatcgctg atgtcgatag tgccgctgga agtagcatca
     9721 attgactgaa tatcctgatc cgcgccagta acgaacgttg ccccatcacc aacccacagt
     9781 gacgaagcat aaggaaagat atccgcaaca tcgcctttca gggtgccttc ttcgacacgt
     9841 accgccgact gcgttgtacc gctggcggtc agctccagcg tacccgcccc cgttttagta
     9901 aatgtgctac cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc gccccactgc
     9961 gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc gcatttcaat atcacgccca
    10021 tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa tctccatcac gctttcttgt
    10081 tacttatcgg taaagataac ctggcggtta tagtgggtat ctccaagctg cgagttgtcg
    10141 ctcaccatca gcgtaccgga ggcgatttgt gttgtgccca aataactatt attgtttgcc
    10201 agacttacct gcccgtcgct cgcatcgacc accacgtaga cgccatcttt cccgtcaccg
    10261 ccgatatgca aattgtcgcc acctgcaaga tctaccacat cctgactgcc gataaccgtt
    10321 ccgctgctgc cactgacttc aaggccatca ttgtatgatg tgccgctctg ccaagtggag
    10381 aaatcagaga gatcgagcac accgccgttt aacacgatcg actgcggatc gtcctggaga
    10441 gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg caccatcatc aacgactata
    10501 tcgccggtta gcgccatcga ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg
    10561 gtgagctgac cagcaccttc gatgatgcca gcaaaactgc cctgattaac agtaacgttg
    10621 ccaccagcat cgatatttaa agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt
    10681 tgttgggtcg agccaacgtt tagctcagcc tgattctgat actgatcaat actccctatc
    10741 gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg tatcgccgac attcatcagg
    10801 gagttgctgc gacccagggt aacttcaccg ttttcaatct gcatctcacc agtaaagtca
    10861 ttgttatctg cattaagtac cagatcgccg gaacctgttt tggtgattaa cccggtacca
    10921 gcaatagagt caacagctcc gtcattctct gtattgccga taaccagcgt ttttccgtcg
    10981 gcaatatcaa aggtaacttc acttaagccg agatacataa agccacccgc cgcagtggaa
    11041 ggaccatctc catagcctgc tgcgctattg ttctcatcga ctaacacgcc gccgttctgg
    11101 ctgtagctgt catcaacaga aatatcaata agatagggag ccctcacgct attggtatat
    11161 atcgccccgc cataaccttc ggctgtgtta tttgtaaagg cggtgttatt tattatcgta
    11221 taacctgaag gatgcttgct gtcgctatta ttatcggtaa catcgattgc cccgccatcg
    11281 ccatcactgt aacttgttga tgtatatgcc tggttattgt taaaaacatc atcacttaaa
    11341 tagacgtcat tattaatggt ataaattgcg ccacctttgc c
//
LOCUS       DAJTYI010000119        11358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYI010000119 DAJTYI010000000
VERSION     DAJTYI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..639
                     /locus_tag="OO564_003152"
     CDS             <1..639
                     /locus_tag="OO564_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654184.1"
                     /translation="KMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHD
                     GSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEY
                     DTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDF
                     SPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(842..1195)
                     /locus_tag="OO564_003153"
     CDS             complement(842..1195)
                     /locus_tag="OO564_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6654185.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1333..2979)
                     /gene="groL"
                     /locus_tag="OO564_003154"
     CDS             complement(1333..2979)
                     /gene="groL"
                     /locus_tag="OO564_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6654186.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3023..3316)
                     /locus_tag="OO564_003155"
     CDS             complement(3023..3316)
                     /locus_tag="OO564_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6654187.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3592..4848
                     /gene="yjeH"
                     /locus_tag="OO564_003156"
     CDS             3592..4848
                     /gene="yjeH"
                     /locus_tag="OO564_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6654188.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4864..5340)
                     /gene="fxsA"
                     /locus_tag="OO564_003157"
     CDS             complement(4864..5340)
                     /gene="fxsA"
                     /locus_tag="OO564_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6654189.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5677..7113
                     /gene="aspA"
                     /locus_tag="OO564_003158"
     CDS             5677..7113
                     /gene="aspA"
                     /locus_tag="OO564_003158"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6654190.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7231..8532
                     /gene="dcuA"
                     /locus_tag="OO564_003159"
     CDS             7231..8532
                     /gene="dcuA"
                     /locus_tag="OO564_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6654191.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8648..8986
                     /gene="cutA"
                     /locus_tag="OO564_003160"
     CDS             8648..8986
                     /gene="cutA"
                     /locus_tag="OO564_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6654192.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GAISLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8962..10659
                     /gene="dsbD"
                     /locus_tag="OO564_003161"
     CDS             8962..10659
                     /gene="dsbD"
                     /locus_tag="OO564_003161"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6654193.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARITGFMDAETFSAHLRDRQP"
     gene            10696..11271
                     /locus_tag="OO564_003162"
     CDS             10696..11271
                     /locus_tag="OO564_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6654194.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2700 a         2955 c         2814 g         2889 t
ORIGIN      
        1 aaaatggcag aaaacatccc agaaattctg gttgatgagc ttaaaaatgc cgatattaat
       61 cgtcgggtta acacgctgga acttaccgat aatcaaggcg aaaatcttac cttcgtatta
      121 acgctacatg acggtagcaa atgtgagtta gtcgtcaatg agttgcagat tgaaatgctg
      181 gcgcgagcaa tcattcatgc aattaataat gcggaaatgc gtgaactggc attacgtatt
      241 acctcactgt tagatttcct gccgttatac gacgttgact gccaggacaa tggcaatctg
      301 gaatatgaca cctattccca accggaatgg aaacataact tgttcgacca ttatctggcg
      361 gtgctttacc gctttaaaga tgaaagtggc aaagaacaat ttagcggcgc agtggtgaaa
      421 acgcgtgaag caacgccggg taaagaaata gaggcgatca ctcgccgaat gcttgatttc
      481 agcccacgac tgaaaaaact cgcgggtgtg ccatgtcagg tctatgctcg caccgtagcg
      541 gcgaataacg cccaaccgct aacccaggat caatgcttac gcgcgctgca tcacttacgt
      601 gtacaatcca ccagcaaaac tgcaccacag gcgaaataat ctcatctttg ctaatgcgag
      661 gctggagcca tccagcctga aatgactgat aacgtgatcg ttggtcatgc cggatgcggc
      721 gtaaacgcct tatccggcct acaaattctc aagcattcaa tcagttgcaa tgcctgctgt
      781 aggcctgata agcgaagcgc atcaggcaat aatacgtttg tcacccagcc tctggatcac
      841 atcagttcgg gcacttataa acctgaccga taatctggct atccgtcggg acaaaactgg
      901 acaacattcc ctgcgacggg ctgctgatgc cataaatcac gttaccgccc attgcagccg
      961 cctggttgcg cagatcgttt gctgcgccgc gcatagaacc gccctcttct ccgtgttgcc
     1021 cggaaagcca gttgctttgc ttacctgtcg cagtaccaat cagctggcac tctgcgcctg
     1081 gctgctcgtc cacaatgcgt acactctgac cggcagcact caattcgttg ctggagctac
     1141 aacccgccat cagtagcgcg gcaccgacaa tccctgctaa gtattttacg tgcatgttat
     1201 tccccataat caatgagctg gacgccactc gcgtcgtccg tgtctgaatc ttatactaga
     1261 aagatgacca aaagaaaaac ccccagacat ttctgcccgg gggtttgttt atttctgcga
     1321 agtgcagggc aattacatca tgccgcccat gccacccatg ccgcccatac cgccagcagc
     1381 gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc atgcattcgg tggtgatcat
     1441 caggccagcc acagaagctg cgtactgcag agcagaacgg gttactttgg ttgggtccag
     1501 gatacccatg tcgatcatgt tgccgtattc ttcggttgct gcgttgtaac cgtagttgcc
     1561 gtcgccgcct ttaacggtgt tagcaacaac agacggttct tcgccgcagt tcaggacgat
     1621 ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg atacccacgt tctggtcttc
     1681 gttctgacca cgcaggtcag ccagtttaga cgctacgcgg atcagcgcaa caccaccacc
     1741 agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc agggcatctt caacgcgtgc
     1801 ttttttctct ttcatttcaa cttcggtagc agcacctact ttgataactg caacgccgcc
     1861 tgccagtttc gctacgcgct cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc
     1921 aatctgctga cggatctgag caacacggcc ctggattgca gcttcttcac ccacgccatc
     1981 gatgatagtg gtggtgtctt tgttgatcac aacacgttta gcctgaccca ggtcttccag
     2041 ggttgctttt tccagctcca taccgatctc ttcagagatt acggtaccgc cagtcagggt
     2101 tgcgatatcc tgcagcatag ctttacgacg atcgccgaag cccggagctt taaccgcagc
     2161 aactttcacg atgccacgca tggtgttaac aaccagagtt gctagcgctt cgccttcaac
     2221 atcttcagcg atgatcagca gcggtttgcc tgctttggca acagcttcca gaaccggcag
     2281 catttcgcgg atgttggaga ttttcttgtc agccagcagg atgaacgggc tttccagttc
     2341 tactgcgcca gtttccggct tgttgatgaa gtaaggagac aggtagccac ggtcgaactg
     2401 cataccttca accacgtcca gttcgtcctg cagaccggta ccgtcttcaa cggtgataac
     2461 gccttcttta ccgactttgt ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga
     2521 gttagcggag atggtaccaa cctgagcaat cgctttagaa tcagagcacg gtacggacag
     2581 cgctttcagt tcttcaactg cagcggtaac cgctttgtcg ataccacgtt tcaggtccat
     2641 cgggttcatg cccgcagcaa cagctttcag accttcagtg atgatagcct gagccagtac
     2701 ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct ttggaggcaa cttctttcac
     2761 catctgcgca cccatatttt cgaacttgtc ttccagttcg atttcacgag caacggaaac
     2821 accatctttg gtgatggtcg gtgcaccgaa agatttatcc agaactacgt tacggccttt
     2881 tggaccgagg gtaactttca ctgcatctgc cagtacgttt acgccgcgca gcattttcac
     2941 acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt atctttattc cttaaattcg
     3001 tatgttcagt gtcgtgcgcg gattacgctt caacaattgc cagaatgtcg ctttcggaca
     3061 tgatcaacac ttcttcattg tcgatcttct cggatttcac accgtagcca tcgttgaaaa
     3121 taacgatgtc gccaactttc acgtccagcg gcttcacttc gccattttca aggatacggc
     3181 cattgccgac agccagcact tcgccgcggg tggatttagc cgctgcagag ccggtcagaa
     3241 cgatgccgcc agcagattta gtttcaactt ctttacgctt gacgatcacg cgatcatgca
     3301 atggacgaat attcattgat aactctcctt tgagaaagtc cgtatctgtt atgggtgacg
     3361 ccggagctta cgtggtttcc cggctggtga ccagagaaat ggggatgagg cttcgcccct
     3421 tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa tcgggtgaaa accctgattc
     3481 acctcacatt tcatcgcaat ttcttcatcg ccgtcataag cgaatctgat tgtgctacca
     3541 tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg gatatcaacc catgagtgga
     3601 ctcaaacaag aactggggct ggcccagggc attggcctgc tatcgacgtc attattaggc
     3661 actggcgtgt ttgccgttcc tgcgttagct gcactggtag caggcaataa cagcctgtgg
     3721 gcgtggcccg ttttgattat cttagtgttc ccgattgcga ttgtgtttgc gattctgggt
     3781 cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg gtatggcgtt tggttcgcgg
     3841 cttgagcgag tcaccggctg gctgttttta tcggtcattc ccgtgggttt gcctgccgca
     3901 ctacaaattg ccgccggatt cggccaggca atgtttggct ggcatagctg gcaactgttg
     3961 ttggcagaac tcggtacgct ggcgttggtg tggtatatcg gtactctcgg tgccagttcc
     4021 agtgctaatc tacaaaccgt tattgccgga cttatcgtcg cactgattgt cgctatctgg
     4081 tgggcgggcg atatcaaacc tgcgagtatc cccttccccg cgccaggaaa tatcgaactt
     4141 accgggttat ttgctgcgtt atcagtgatg ttctggtgtt ttgtcggtct ggaagcattt
     4201 gcccatcttg cctcggaatt taaaaatcca gagcgtgatt ttcctcgtgc tttgatgatt
     4261 ggtctgctgc tggcaggatt agtctactgg ggctgtacgg tagtcgtctt acacttcgac
     4321 gcctatggtg aacaaatggc ggcggcagca tcgcttccaa aaattgtagt gcagttgttc
     4381 ggtgtaggag cgttatggat tgcctgcgta attggctatc tggcctgctt tgccagtctt
     4441 aacatttata tacagagctt cgcccgcctg gtctggtcgc aggcgcaaca taatcctgac
     4501 cactacctgg cacgcctctc ttctcgccat atcccgaata atgccctcaa tgcggtgctt
     4561 ggctgctgcg tggtgagcac gttggtaatt catgccttag agatcaatct ggaggctctt
     4621 attatttatg ccaatggcat ctttattatg atttatctgt tatgcatgct ggcaggctgt
     4681 aaattattgc aaggtcgtta tcgactactg gcggtggttg gcgggctgtt atgcgttctg
     4741 ttactggcaa tggtcggctg gaaaagtctc tatgcgctga tcatgctggt ggggttatgg
     4801 ctgtttctgc caaaacgaaa aacgccggaa aatggcataa ccacataatc cggcgtttcg
     4861 acattaatcc tggcgatcgt ctttatgatc aaggcggtcg cggtcatcat cctttcgctg
     4921 atactcacca tcaaaagtat taccgccacc ggtcccggcg ctaaaaccgc cgcctggcat
     4981 gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga tgcttttgca ccggcggcaa
     5041 taaaagcaga agaccgagga agtcggtgaa aaagcccggt aataaaagca gcaaaccggc
     5101 aataatcagc gaaacacttt taatcatctc cgccgcaggg ttttcacccg ccgccatttt
     5161 ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt accagtgaca taccgataac
     5221 tgacgtgaat ataaccagca cgagggtcag caataccccc aatacatggg caacctgaat
     5281 aaagattgaa atctcaatat agacataaag gaaaatggca ataaaaggta accagcgcaa
     5341 aggtttctcc tgtaatagca gccggttaaa cccggctacc tgaatgggtt gcgaatcgcg
     5401 ttcagcttat attgtggtca ttagcaaaat ttcaagatgt ttgcgcaact atttttggta
     5461 gtaatcccaa agcggtgatc tatttcacaa attaataatt aaggggtaaa aaccgacact
     5521 taaagtgatc cagattacgg tagaaatcct caagcagcat atgatctcgg gtattcggtc
     5581 gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc acatatattc tgtgtgttta
     5641 aagcaaatca ttggcagctt gaaaaagaag gttcacatgt caaacaacat tcgtatcgaa
     5701 gaagatctgt tgggtaccag ggaagttcca gctgatgcct actatggtgt tcacactctg
     5761 agagcgattg aaaacttcta tatcagcaac aacaaaatca gtgatattcc tgaatttgtt
     5821 cgcggtatgg taatggttaa aaaagccgca gctatggcaa acaaagagct gcaaaccatt
     5881 cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg aagtcctgaa caacggaaaa
     5941 tgcatggatc agttcccggt agacgtctac cagggcggcg caggtacttc cgtaaacatg
     6001 aacaccaacg aagtgctggc caatatcggt ctggaactga tgggtcacca gaaaggtgaa
     6061 tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc agtccactaa cgacgcctac
     6121 ccgaccggtt tccgtatcgc agtttactct tccctgatta agctggtaga tgcgattaac
     6181 caactgcgtg aaggctttga acgtaaggct gtcgaattcc aggacatcct gaaaatgggt
     6241 cgtacccagc tgcaggacgc agtaccgatg accctcggtc aggaattccg cgctttcagc
     6301 atcctgctga aagaagaagt gaaaaacatc caacgtaccg ctgaactgct gctggaagtt
     6361 aaccttggcg caacagcaat cggtactggt ctgaacacgc cgaaagagta ctctccgctg
     6421 gcagtgaaaa aactggctga agtcactggc ttcccatgcg taccggctga agacctgatc
     6481 gaagcgacct ctgattgcgg cgcttatgtt atggttcacg gcgcgctgaa acgcctagct
     6541 gtgaagatgt ccaaaatctg taacgacctg cgcttgctct cttctggtcc acgtgccggc
     6601 ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt ccatcatgcc agctaaagta
     6661 aacccggttg ttccggaagt ggttaaccag gtatgcttca aagtcatcgg taacgacacc
     6721 actgttacca tggcagcaga agcaggtcag ctgcagttga acgttatgga accggtcatt
     6781 ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg cttgctacaa cctgctggaa
     6841 aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg aaggttacgt ttacaactct
     6901 atcggtatcg ttacttacct gaacccgttc atcggtcacc acaacggtga catcgtgggt
     6961 aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg ttctggaacg cggtctgttg
     7021 actgaagcgg aacttgacga tattttctcc gtacagaatc tgatgcaccc ggcttacaaa
     7081 gcaaaacgct atactgatga aagcgaacag taatcgtaca gggtagtaca aataaaaaag
     7141 gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac ttaaaaataa caatctaata
     7201 tcaacttgtt aaaaaacaag gaaggctaat atgctagttg tagaactcat catagttttg
     7261 ctggcgattt tcttgggcgc cagattgggg ggaataggta ttggttttgc aggcggattg
     7321 ggggtgctgg ttcttgccgc tattggcgtt aaacccggta acatcccgtt cgatgtcatt
     7381 tccattatca tggcggttat cgccgctatt tctgccatgc aggttgctgg cggtctggac
     7441 tatctggttc atcagacaga aaagctgctg cgccgtaacc cgaaatacat cacgatcctc
     7501 gcaccgatcg tgacctattt cctgactatc tttgctggta ctggcaacat ctctctggcg
     7561 acactgccag ttatcgctga agttgcgaag gaacaaggcg ttaaaccttg ccgtccgctc
     7621 tctactgcag tggtatccgc gcagattgcg atcaccgcat cgccaatctc agcggcagtg
     7681 gtttacatgt cttccgtgat ggaaggtcat ggcatcagct acctccatct gctctccgtg
     7741 gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct tcctggtcac tatgctgttc
     7801 aactccaaac tctctgacga tccgatttat cgcaagcgtc tggaagaggg cttggttgaa
     7861 ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc
     7921 ctgctgggcg tagttggcgt ggttatctat gcaatcatca acagcccaag catgggtctg
     7981 gttgaaaaac cgctgatgaa caccaccaac gcaatcctga tcatcatgct cagcgttgca
     8041 actctgacca ccgttatctg taaagtcgat accgacaaca tcctcaactc cagcaccttc
     8101 aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg cgtggctggg cgatactttc
     8161 gtttccaaca acatcgactg gatcaaagat accgctggtg aagtgattca gggtcatccg
     8221 tggctgctgg ccgtcatctt cttctttgct tctgctctgc tgtactctca ggctgcaacc
     8281 gcaaaagcac tgatgccgat ggctctggca ctgaacgttt caccgctgac cgctgttgct
     8341 tctttcgctg cggtgtctgg tctgttcatt ctgccgacct acccgacgct ggttgctgcg
     8401 gtacagatgg atgacacggg tactacccgt atcggtaaat tcgtcttcaa ccatccgttc
     8461 ttcatcccgg gtactctggg tgttgccctg gccgtttgct tcggcttcgt gctgggtagc
     8521 ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg ctttctttcc cgccgactaa
     8581 catccttccc ccgtccgttg tatagtgacc tctctcttgc ggttccatct gttcttgcga
     8641 ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct gtcgtggtgc tatgtaccgc
     8701 accggatgaa gcgacagccc aggatttagc cgccaaagtg ctggcggaaa aactggcggc
     8761 ctgcgcgacc ttgatccccg gcgctatctc tctctattac tgggaaggta agctggagca
     8821 agaatacgaa gtgcagatga ttttaaaaac taccgtatct caccagcagg cactgctgga
     8881 atgcctgaag tctcatcatc catatcaaac cccggaactt ctggttttac ctgttacaca
     8941 cggagacaca gattacctct catggctcaa cgcatcttta cgctgattct gctactttgc
     9001 agcacttccg tttttgccgg attattcgac gcgccgggac gttcacaatt tgtccccgcg
     9061 gatcaagcct ttgcttttga ttttcagcaa aaccaacatg accttaatct gacctggcag
     9121 atcaaagacg gttactacct ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa
     9181 attgccgacg tgcagctgcc gcaaggcgtc tggcatgaag atgagtttta cggcaaaagc
     9241 gagatttacc gcgatcggct gacgcttccc gtaaccatca accaggcgag tgcgggagcg
     9301 acgttaactg tcacctacca gggctgtgct gatgccggtt tctgttatcc gccagaaacc
     9361 aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag cgtcacagcc tgtgtctgtt
     9421 tcgcagcaag agcagcacac cgcgcaattg cccttttccg cgctctgggc gttgttgatc
     9481 ggtattggta tcgcctttac gccatgcgtg ctgccaatgt acccactgat ttctggcatc
     9541 gtgctgggtg gtaaacagcg gctctccact gccagagcat tgttgctgac ctttatttat
     9601 gtgcagggga tggcactgac ttacacggcg ctgggtctgg tagttgccgc cgcagggtta
     9661 cagttccagg cggcgctaca gcacccatac gtgctcattg gcctcgccat cgtctttacc
     9721 ttgctggcga tgtcaatgtt tggcttgttt actctacaac tcccctcttc gctgcaaaca
     9781 cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac ctggcggtgt gtttgttatg
     9841 ggggcgattg ccggactgat ctgttcacca tgcaccaccg caccgcttag cgcgattctg
     9901 ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg gcacgcttta tctctatgcg
     9961 ttgggcatgg gcctgccgct gatgctaatt accgtctttg gtaaccgctt gctgccgaaa
    10021 agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg
    10081 gtcttcctgc tggagcgagt gattggtgat gtatggggat tacgcttgtg gtcggcgctg
    10141 ggtgtcgcat tctttggctg ggcctttatc accagcctac aggctaaacg cggctggatg
    10201 cgcgtggtgc aaataatcct gctggcagcg gcattggtta gcgtgcgccc acttcaggat
    10261 tgggcatttg gtgcgacgca taccgcgcaa actcagacgc atctcaactt tacacaaatc
    10321 aaaacgatag atgagttaaa tcaggcgctc gttgaagcca aaggcaaacc ggtgatgtta
    10381 gatctttatg ccgactggtg cgtcgcctgt aaagagtttg agaaatacac cttcagcgac
    10441 ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc aggccaacgt cacggccaac
    10501 gacgcacaag atgtggcgct gttaaagcat cttaatgtcc ttggcctacc gacaattctc
    10561 ttttttgacg gacaaggcca ggagcatcca caagcacgca tcacgggctt tatggatgct
    10621 gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag
    10681 acggaggaaa atagcgtgca acgtgaagat gtactgggag aagccctgaa attattagaa
    10741 ttacaaggga ttgccaacac cacgctggag atggttgctg aacgtgtgga ttatccactg
    10801 gacgagctac gccgcttctg gccagacaaa gaggcaatcc tctacgatgc gctgcgttat
    10861 cttagccaac agatagatgt ctggcgtcgt cagctgatgc tggacgaaac gcaaaccgcc
    10921 gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat
    10981 ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg atcctggcca ccctattcat
    11041 caactggccg atcagcaaaa aagcgcggcc tacgatttca cccacgaact gttaaccacg
    11101 ctggaagttg acgatccggc gatggtagca aagcagatgg aactggtgct ggaaggctgt
    11161 ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg acactgcaca tcggctggcg
    11221 gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg cattgacctg acagaaacac
    11281 agaaaagaag cgatttgccg caatcttaag cagttgaatc gcttttactg aaattaggtt
    11341 gacgagatgt gcagatta
//
LOCUS       DAJTYI010000120        11204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYI010000120 DAJTYI010000000
VERSION     DAJTYI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..612
                     /gene="phnP"
                     /locus_tag="OO564_003163"
     CDS             <1..612
                     /gene="phnP"
                     /locus_tag="OO564_003163"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6654195.1"
                     /translation="ITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVG
                     DPIPIYGPPDDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYL
                     LETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLAL
                     NQVIRSPRVILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(759..1088)
                     /locus_tag="OO564_003164"
     CDS             complement(759..1088)
                     /locus_tag="OO564_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654196.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1157..1789)
                     /locus_tag="OO564_003165"
     CDS             complement(1157..1789)
                     /locus_tag="OO564_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6654197.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(1870..4085)
                     /locus_tag="OO564_003166"
                     /pseudo
     CDS             complement(1870..4085)
                     /locus_tag="OO564_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            4199..5719
                     /locus_tag="OO564_003167"
     CDS             4199..5719
                     /locus_tag="OO564_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6654198.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            5716..6690
                     /locus_tag="OO564_003168"
     CDS             5716..6690
                     /locus_tag="OO564_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6654199.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            6721..7662
                     /locus_tag="OO564_003169"
     CDS             6721..7662
                     /locus_tag="OO564_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6654200.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            7717..8400
                     /locus_tag="OO564_003170"
     CDS             7717..8400
                     /locus_tag="OO564_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR6654201.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            8415..9275
                     /locus_tag="OO564_003171"
     CDS             8415..9275
                     /locus_tag="OO564_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR6654202.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            9272..10273
                     /locus_tag="OO564_003172"
     CDS             9272..10273
                     /locus_tag="OO564_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6654203.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(10270..10976)
                     /locus_tag="OO564_003173"
                     /pseudo
     CDS             complement(10270..10976)
                     /locus_tag="OO564_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         2479 a         3124 c         3081 g         2520 t
ORIGIN      
        1 atcaccctga tcgacgccgg gctgcacgat ctcgccgatc gctggtcgcc cggatcgttc
       61 cagcagtttt tgctgacgca ttatcatatg gatcacgccc aggggctgtt tccgctgcgc
      121 tggggcgtgg gcgatccgat cccgatctac ggcccgccgg atgaccaggg ttgcgacgat
      181 ctgtttaaac atccgggcct gcttgatttc agccacacag tggaaccgtt tgtggtgttt
      241 gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc actcaaaact gaccttcggt
      301 tatctgctgg aaacggcaca tagccgggtg gcgtggctat ctgacaccgc tggcctgccg
      361 gaaaaaacgc tgaaattttt acgcaataat cagccacagg taatggtgat tgattgcagc
      421 cacccgccgc gcgcggatgc gccgcgtaat cactgtgatt taaataccgt gcttgcgctg
      481 aatcaggtta tccgctcgcc acgggtgatt ctgacccata tcagccacca gtttgatgcg
      541 tggctgatag aaaacgcact gccgtcgggg tttgaggcgg ggtttgatgg gatggagatt
      601 ggggtggcgt gatgagaggg aatgtgcgcg ctggtcccct caccctaacc ctctccccag
      661 aggggcgagg ggagtgattg tgctcgatat tgaatattgc gctggttttc tccctctccc
      721 cattggggtg aggggcgatg cctgctccat acccaacctc atcgcccata ctcatcttcc
      781 attctccgct cttcatcttc tagctgccga cgctcctgat caagctggcg ctggcgatcg
      841 tccagctgcc tgcggcgatc ttcaaactgg cagcggcggt cgtcatattg tctgcgccga
      901 tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat cttcatagtc tcgcccacgg
      961 tcagagttat aagcgtcatt aatcgcctgc tgaatattac caatagtatc gtcgataata
     1021 tcggcctggg ccggaacggt ggacagcgtg agcagggtga aaagaaaaag cgtggagtaa
     1081 ggtttcataa gccagtctct gcgatggact ggcttaacgt tagttgagtg tgaatgaccg
     1141 gagtagcgga gtaatctcaa attcggtaag gcgcagactc ttccattagc tggttagctc
     1201 gctgtttatt ttccgccacc aatacaatgc catgacattt ttgatccggt gaaatactcc
     1261 agatacgctc ttccggaatg cagcagccat cgtagatcac tttcgtgcca tcgtagggcc
     1321 aaaatgcccc ttgttcagtg tctaatacaa acacggaaat ctgatcggac tcgcgcgcga
     1381 tcttctgcca gagcatcttc ccgccatcat attgatcaaa atcgctgatt agcgtgatac
     1441 cgcattgcag gattagcgct tcatacagcc gagttgccag acccttatcg cgctcacctg
     1501 gagagacaca aataccttcc acctgtacca gatccgagtc ttcccaccag cgttcaacgg
     1561 caatgatatc gctgccgaac tcttttttac gctgcagatc gagagcggca agtacccgat
     1621 gacgcggcac gcctgcaaaa acttcaatgt ccgcttcaat gatggccgct cttggcgtct
     1681 ggttgtcata aattacctgt aacagacgtc cagaaagctc gatctcttcc accacgcgaa
     1741 aagaacgagc aaccgtcata gggctatagg tggttggcat cacgatcata tgtaaatcac
     1801 ttcaattcag aacagaattg ctcacaatag cagcacgttt gcttcgagcc tcttgataaa
     1861 ttctgaaaat catccctcaa tttgccccag cacctgcgcc agctgcgcgc gggtaaaggg
     1921 tttgcgcagc agcgccacgt ccggcagcgc ggggttatgg ctggggcgta aatcctgacc
     1981 gctgatgagt agcagcgtca ggtgcgggta aagtttgcgg gccgcattga ccacttctgc
     2041 gccgctcatg ccgccagcag gtccagcgcc tgttcgccgc tgctcgcttc cagtgtcaaa
     2101 tagcccagca ggtgcaactg ctcgcagatg gtctggcgca ccgcggcttc atcttccagc
     2161 actaacacca gtttatcgcc attaacagct gtctgttcta ccgccgctgg cgataggttt
     2221 ttcgccgccg tcagcgcgcg gggaagctgt aatctgaccg ttgtcccctg ccccggcgcg
     2281 ctttcaattt ccacgcgccc gccggactgg cgcacgaaac catagaccat cgacaagccc
     2341 agcccgctgc cgctccccgt ctgtttggtg gtaaagaacg gctcgaacac ctggcttttc
     2401 accgcctgcg acataccgct gccatgatcg atgacctcca gtgccaccat gtcctgacgc
     2461 tgcccactgc tgcgggtaac acgctgattc caggtacgaa ttttgataat tcccgtctgc
     2521 ccttccatcg cgtcgcgggc gttcatcacc aggttgatga tggcgttttc cagttggctg
     2581 acgtcaatcc acgcggacca cgccggagac tgcgcttcaa tctcaatcgt cagcgtcgcc
     2641 ggtaaggagt ggcgcattaa ttcgcttaac tcttccagca gtgtttttag ttcaacggct
     2701 tgcggatgta aggactgctt gcgggaaaat gccaacaatc gctgggtcag tagcgcgcca
     2761 cgttcggcgg cttgctgcgc ccgctgtatt cgcggcgcgt cgggtgagtt cgtgttcacc
     2821 agttccaggc taccaataat taccgccagc agattattaa aatcatgggc cagcccgccc
     2881 gtcagttgcc tgaccgcttt taacttctgg ctgtgcaata gcgcctcttc cagcccctgc
     2941 cgctcggttt gggcaatctc catctgcgtg gttttctctt tcaacagccg tgacgttcgt
     3001 tcaagtgatg cggtattgcg ggcaaaaacg ttaaaggcgc gcgccagttc acccaactca
     3061 tcgcgccgct gtaacgccgg aaaagtgacg tccgcttcgc cgtgcgccag gcggctcatc
     3121 gcgcgggaaa tcgccgtcag gttagagccg agattgcgat agatatacca acctgcaaac
     3181 gcggtgatca ccaccgctaa cagggcgaaa atcatgatca gcctctcgcc ggagcgcaaa
     3241 tcctggtaac tttgctccac ccgctgacca gagagcgtcg ccacctgttc ggcgtaacgg
     3301 ttgatgtcgc cattaaggat cgccaccagc gctttgatgt gaaacatata ccagctgatt
     3361 gccagatcgc tttgctccag agccgccgaa agtggcgcga gtttacgcag ttcacgatta
     3421 aaatcagcga gtagcgtatt aaccaatggc gtatcgctgt gagttggcaa ggcagacatc
     3481 actccgacca gttgatgaat aatcgcctgc ggcgcggggg tggcaatggc ggcaacaatc
     3541 agtcggttca tctctttaag caaaatatta tcttgcgcgt gagtgacttt ttgcagatgc
     3601 cgcagataac ttaagttttg atacagcgaa cttaacagcg cgttacgctc cagatgccgc
     3661 ctttgcccgc gttccagcat tccgccgacg ctggtttgca gttcgttgct acgcgtgatg
     3721 atgcgcgtca ccagcccggc ttcctgctgc gctaaaggcg cgttcgccag ttgtgcaagc
     3781 gagcttttta atgcccgctg tgtctcctgc aaccgctcgg cttcgccttt atattccagt
     3841 gctcccacca cctgcgacaa tcgcactgcc gccgttgcca cgtttgccgt gtcgcgcgcc
     3901 agattcaggc tgccggtcat atcatctaca gtttgctgct tcaccagctc ttgcatctgg
     3961 ctggcatgac gaaagcccat taccgccact ccgctgacca tcaacgtcac cgccaccacc
     4021 agcaaattca aaagcagcaa tcgcccgcgc gcgctggcaa aaaagtgacg tccggggtgc
     4081 gccatatcac gctccgtgaa gaaaaatcgc aatatgacaa atatgaacgg cttctgacat
     4141 tttgcattta ctttcctgtg atggagtgct gatatcgaca acctcgcagg agtccaccat
     4201 gaacagcacg ccggttcttg agatgcgcaa tattgccaaa gccttcggca aattttatgc
     4261 gctcaaaggg gtggatttga cggtctaccc tggcgagatc catgccctga tgggggaaaa
     4321 cggcgcggga aaaagcacgc tgatgaaggt gctggcgggg gcgtataccg ccaccagcgg
     4381 cgagatcctg atagacggca agccctttca cattcgcacg ccaaaagatg ccttaagcgc
     4441 cggtattacg ctgatttatc aggagatgca gctggcaccc aatttgacgg tggcagaaaa
     4501 tattttcctc ggcagcgagc tttcccacgg cgggctggtg cagcgaaaag agatgctggt
     4561 gcaggcgcaa agagtgatcg accgcctcgg cgcgcagttt aacgccagcg ataaggtcat
     4621 gacgctgacc attgccgaac aacagcaggt cgaaatcgcc cgcgcgctac atcgcaacag
     4681 ccgcattctg gtgatggacg aacccaccgc tgccctctcc tcccgtgaaa ctcaccgtct
     4741 ttttgaactg attatgcggc tgcgcgatga agggatggcg attatctaca tcagccaccg
     4801 tatggcggaa gtgtatgagc tttccgatcg cgtcagcgtg ctgcgcgacg ggcaatacgt
     4861 tggcagcctg acccgcgata acctcaatgc cggggagctg gtgcggatga tggtcggcag
     4921 gccactgagc gatctgttca ataaagagcg cgatatcccg ctcggtaaag cccgcctgaa
     4981 tgttcaccac ctgaccgacg gcggcaaagt ccagccgagc tgcctgcagg tgctttccgg
     5041 cgaaattgtt ggcctcgccg ggctggtggg agccggacgt tccgaactgg cgcagttaat
     5101 tttcggcgtg cggaaagcga caggcggaat gattgaagtc gatggcgaac cggtggtgat
     5161 ccactccccg cgcgaagcca tcgatcttgg cattggtttt ctcaccgaaa accgcaaaga
     5221 acaaggctta ttccttgaaa tggcggcagt cgaaaacatc actatggcaa ccctggagcg
     5281 cgatgccagc tggggaatgc tcaatcgcaa aaaagcgcaa accatttccg atgacgccat
     5341 taagttgctc aacattcgcg tgcctcatgc ccaggtacgc gcgggcgggc tttccggcgg
     5401 caatcagcaa aaactgttga tctcccgctg ggtggcgatt ggcccgcgca ttttactgct
     5461 cgatgaaccc acccgcggcg tggacgttgg cgcaaaaagt gagatctacc ggatcatgaa
     5521 cgagatggcg cgcaagggcg tggcgatcct gatgatctcc agcgaactgc cggagatagt
     5581 gggaatgagc gatcgcgtcc atgtgatgcg cgaaggcagc attgccggtg agctaaatgg
     5641 caaaaacatc acccaggaaa acattatgac cttagcgact ggcgtcaacg acgcccattc
     5701 ccaggcggtg acctcatgac gacctctgca aaaacctctg ccaaaaaaat gctgatgagc
     5761 gatctgatgc aaacggtcgg cattttgccc attttgatcc tgatcgtagc ggtatttggc
     5821 tttatcgcgc caaacttttt taccgaaagc aacctgctaa atattacccg ccaggcgtcg
     5881 atcaacattg tgctggcggc ggggatgacc ttcatcattt tgaccggtgg gatcgatctt
     5941 tctgtaggtt caatcctcgg caccacggcg gtggcggcaa tggttgtctc ccttagcccg
     6001 gagttcgcca tgttgtctat cccggcggcg ctgatgctcg gcctgctgct ggggctgttc
     6061 aacggcgcgc tggtggcctt tgccggattg ccgccgttta tcgtcacgct tggcacctat
     6121 acggcgctgc gcggggcggc gtatctgctg gctgatggca ccacggttat caactccaac
     6181 attaatttcg agtggatagg caataactat ctcgggccgg tcccgtggct ggtggtgatt
     6241 gcgctggcgg tgatcgtgct gtgctggttt attctgcgtc gcaccacgct tggcgttcat
     6301 atctacgccg tcggtggcaa tatgcaggcg gcgcgtctta ccggcattaa ggtctggctg
     6361 gtgctgctgt ttgtttatgg catgagcggc ctgctttcag ggcttggcgg gattatgagc
     6421 acctcgcggc tgtacagcgc caacggcaac ttaggtatgg gttacgaact ggatgccatt
     6481 gcggcggtga tcctcggcgg caccagtttt gtcggcggca tcggcaccat caccggcacg
     6541 ctggtggggg cgctaattat cgccacgctc aacaatggca tgaccttaat gggcgtctct
     6601 tacttctggc agctggtgat caaaggggcg gtgatcatca ttgcggtgct tatcgacaaa
     6661 taccgtaccc gacatcatca aagtgcataa caacaatacc ctacacatga ggaatacagc
     6721 atgagattga agcccttagt gaccgcgctc tgtgcaggcg cgctgctggc cgcagcaccg
     6781 ttcgcccagg cgaaagatct gaaatcgata ggcgtaacgg taggtgacct ggcgaacccg
     6841 ttcttcgtac agatcaccaa aggcgcggag ctggaagcac gcaaactggc gggcgataac
     6901 gtcaaagtga cgctggtttc cagcggctac gatctgggtc agcaggtctc gcagatagac
     6961 aactttatcg ccgctaacgt cgatatgatc atccttaacg ctgccgattc aaaagggatc
     7021 ggcccggcgg tgaaacgggc aaaagacgcc gggatcgtgg tggtggcggt tgacgtggcg
     7081 gcagaaggtg ccgatgccac catcacttcc gataacaccc aagcaggcga aatggcctgt
     7141 aaatacatca ccgatcgcct gaaaggcaaa ggcaacgtgg tgatcatcaa cggaccgccg
     7201 gtgtccgccg tgcaaaaccg cgtcgaaggc tgccagaccg aatttaaacg ccatcctgac
     7261 atcaaggtgc tctctgacaa caagaacgcc aaaggcagtc gtgagggcgg gctggaggtg
     7321 atgaccgggc tgctggcggc aaatccgaag atcgacggcg tatttgcgat taacgatccg
     7381 acagcgatcg gtgccgatct ggcagcaaaa caggcgcaac gtaacgagtt ctttattgtc
     7441 ggcgtggatg gaagcccgga cgccgaagag gcgctcaagc gtgagaactc gttgtttgtc
     7501 gctactccgg cgcaagatcc acaggtgatg gcagcgaaag cggttgagat cagttatgac
     7561 attttgcagg gcaaacctgc gccgaaagag ccggtgctga tcccggtgac gatgatcgat
     7621 aaaaacaacg tcggcaatta caaaggctgg acggtgaaat aagtccacac gccgggcggc
     7681 gatgctgccc ggccaccgct aaggagaacg ctttcgatga aacgctccgc tataaacaac
     7741 attctcggcc atacacggca attcttttcg caacatgacg tacatctgcc gccctttgcc
     7801 agtttctctc ctgctcaatg gcagcagctc gacaccgccg cgtgggaggt ggtttttgat
     7861 ctcaaactcg gctgggatgt taccgctttt ggtggtaaca actttgcagc acagaggctg
     7921 acgctgttta ccctgcgtaa cggctcagca aaaggaatgc cgtatgtgaa atgttacgcc
     7981 gagaagatca tgcatgtgcg cgatgcgcag gtcacgccaa tgcattttca ctggcgtaag
     8041 cgtgaggaca tcatcaatcg cggcggcggc aatttgattg ttgaactgtg gaatgccgat
     8101 agcaacgagc aaacagttga taccgacgtt acggtggtga tcgacggctg ccgccagact
     8161 catgtcgcag gcagtcagtt acgcctgtcc cccggcgaaa gtatctgcct gccgcccggc
     8221 ctgtatcaca gcttttgggc ggaaaccggt tttggcgatg ttctggttgg cgaagtctct
     8281 tctgtcaatg acgacgacca cgataaccac tttttacaac cgctggaccg ctacaacctg
     8341 attgacgaag acgaaccggc ccagctggtg ttgtgtaacg aataccgcca gttcctctga
     8401 ataaggataa cgttatgccg ttgatttctc tcgctgaagg gctggcacat gcccgcgaac
     8461 atcactatgc gttaggtgcg tttaatgtgc tggactcaca ctttttacgc gccttgtttg
     8521 ctgctgccag acaagaacgt tcgcccttta ttatcaacat tgccgaagta cattttaaat
     8581 atatctcgct ggattccctg gtcgagacag tgaaatttga agcggcgcgt caagcgattc
     8641 cggtggtatt aaatctcgat cacgggctgc attttgaatc ggtggcgcaa gcgttgcggc
     8701 tgggattcag ctcggtaatg tttgatggct ccacgctggg gtatgaagag aatatccgcc
     8761 agacgcggga agtggtgaaa atgtgccatg ccgttggcgt atcggtagaa gctgaactgg
     8821 gcgcggtggg tggtgacgaa ggcggtgcgc tttacggtca tgccgatgaa tctctgttta
     8881 ccgatccgca gctggcgcgt gattttgtcg accgtaccgg cattgacgcg ctggccgtgg
     8941 cgattggcaa tgcgcacggc aaatatcgcg gcgaaccgaa actcgatttc ccgcgcctcg
     9001 acgctatccg ccagcaggcg gctatcccgc tggtattaca cggcggttca gggattagag
     9061 atgccgattt ccgccgcgcc atcgaactgg gtatccacaa aatcaatttc tacaccggca
     9121 tgtcacaagc ggcgctggct gccgttgagg cccgaatggc gcaccgtcag ccgatgtatg
     9181 acgagtttgc cgagttacta ctagcagtgg aagccgccat cagcgatacg gttgcacaac
     9241 agatgcgtat tttcggcagt gcggggcagg catgatggaa cgtaaaggga ttatcgccgc
     9301 aggcaatatg ctggttgacc acgtccacca gattatccag tggccagagc gcggctggct
     9361 ggcggagatt acccacagcg aacgctccac cggcggcgcg ccgcttaacg tcctgctgac
     9421 actggcgaaa atgcgcaccg ggttgctgtt acaggcggtc gggctaatcg gtcaggacag
     9481 cgacggcgat tacattatgg cgatgcttga gcaatatcac gtcaatcgtc agcatgtgca
     9541 gcgcactacc tttgccccga cgtcaatgtc gcaggtaatg actgatccaa gcgggcagcg
     9601 gacgtttttc cattcgccag gtgcaaacag acttttggat cttccggcgt tcgatcagct
     9661 cgataacacc atgaagattt tccatctcgg ctatttactg ttgctcgaca gcctggatat
     9721 gccggacgat gtttacggca cccgtagcgc acgcctgctg gcgcaaatgc gtgatactgg
     9781 ctttgaaacc tcactcgacc tggtctcccg caagggcgat ccgcgttatc agccgctggt
     9841 gttgcccgcg ttgcgccatc tggattacct ggtaattaac gaactggagg ccggggagtt
     9901 tagcgggctg gaaattcggt tacccaacgg cgaaccgcat atcgcgcata ttgctgccgc
     9961 cgcgcgtgaa ctgttagatg cgggcgtgcg ccagcgtgtg gtgatccact gcccggaagg
    10021 ggcgtgggga gagaccccgg aacagtgcgg cgtgtggatc ccttcgcaaa agttagaaca
    10081 acgggaaatt atcggcagcg tcggtgcggg cgatgtattt tgtgccggat ttttatatgg
    10141 ctgccatgaa cgctggacgc ttaccgagag catcaaactg gcacacgcct gcgcgcgagc
    10201 aagcctgtta tgcgccaatg ccatcgacgg ggcgaaaacg ctggaagagc tgcaaacaga
    10261 aatgtcggat taatgcacgt cggcagcgaa gacatacccc agaccgcgca gagttttaat
    10321 caacatcggc tgatgcgggt tgagttcgat tttgcgtcgt aagcgcatga ttaatacgtc
    10381 gatggtgcga tcaaacacct ccacgctgtc gctatgagtg aacgccagca gctgttcgcg
    10441 gcttaatacc cgacgggcat tttgagccag ggccagtaac aagccatatt cgccctgagt
    10501 cagctcgaca tgttgttgta gcggactatg cagttggcag cgaatggtat cgagatacca
    10561 gccattgaat gacagcccgg cgtttttctc ctcattaacc gccagcgcgc caacccgtcg
    10621 caacacgctt tcagtcgtgc gaccaccacc cgtggggtga acggcttgca gatgtaatca
    10681 tccgcgccca tttccagccc gaccaccaca tcagactcac tccccattcc ggtcaacatc
    10741 accacaggca atgccggacg cagtttctgg atttgctgca ataccctcag gccgttggta
    10801 tcgggcagca tcatatccag caccaccagc gcaatgtcag gctcgccctc aatacgcgat
    10861 atggcctcct gtccggtatg gctgactgaa accgtaaaga catgttcact taacacgtcc
    10921 tgcaacagtg cgcagatagc cgtgtcatca tccaccacca gcacgacggg tttcatggtt
    10981 acactccgca tcattcatct aattaatcat tgtgcgtact tcttgtcgaa gtgaaagcgg
    11041 aaagtaatat aaataataaa aatataagtc aggtcacatt attgatgata gtaataaata
    11101 tgccgtgctt ttccctcttc agcacaaatg cctaataaaa tggcgcgttc cgctcacact
    11161 tccacttatt aaagtcgcaa attcccaatt atgataaccc cagc
//
LOCUS       DAJTYI010000121        11193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYI010000121 DAJTYI010000000
VERSION     DAJTYI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            25..384
                     /locus_tag="OO564_003174"
     CDS             25..384
                     /locus_tag="OO564_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6654204.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(504..683)
                     /locus_tag="OO564_003175"
     CDS             complement(504..683)
                     /locus_tag="OO564_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6654205.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            757..2118
                     /gene="pabB"
                     /locus_tag="OO564_003176"
     CDS             757..2118
                     /gene="pabB"
                     /locus_tag="OO564_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6654206.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2122..2700
                     /locus_tag="OO564_003177"
     CDS             2122..2700
                     /locus_tag="OO564_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6654207.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2884..4248
                     /gene="sdaA"
                     /locus_tag="OO564_003178"
     CDS             2884..4248
                     /gene="sdaA"
                     /locus_tag="OO564_003178"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6654208.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDNVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4379..5977
                     /locus_tag="OO564_003179"
     CDS             4379..5977
                     /locus_tag="OO564_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6654209.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5981..7531)
                     /gene="yoaE"
                     /locus_tag="OO564_003180"
     CDS             complement(5981..7531)
                     /gene="yoaE"
                     /locus_tag="OO564_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6654210.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            7994..8965
                     /gene="manX"
                     /locus_tag="OO564_003181"
     CDS             7994..8965
                     /gene="manX"
                     /locus_tag="OO564_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6654211.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9028..9828
                     /gene="manY"
                     /locus_tag="OO564_003182"
     CDS             9028..9828
                     /gene="manY"
                     /locus_tag="OO564_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6654212.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9841..10692
                     /gene="manZ"
                     /locus_tag="OO564_003183"
     CDS             9841..10692
                     /gene="manZ"
                     /locus_tag="OO564_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6654213.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10747..>11193
                     /locus_tag="OO564_003184"
     CDS             10747..>11193
                     /locus_tag="OO564_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6654214.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
BASE COUNT         2749 a         2826 c         2824 g         2794 t
ORIGIN      
        1 tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct tatcgataac
       61 atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct ggatttcatt
      121 ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac ggcgaaggga
      181 atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat tacggcgcga
      241 gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg ggcaaaaaaa
      301 atggagacag gtgaacgctc tgtgattaaa aatcctgaat acttttcaac atatatgcag
      361 gaagaactaa aagcactggt ctgagttaaa tttatatcag cataaatggg tcaggacgct
      421 tttaatcaca tataaaaagc gtcctttttc tcacaatcaa atgatcatcg gcttctataa
      481 cgaaatcgat acggattagt gtgttactcg tcttcatcct caaaacgcgc cacgattagc
      541 tcgccggagt ggttggcacg cagctcttct gccaccagcg cgattgcctg gccgctactc
      601 atcccttgtg ccatcagttc ctggatccgc tcgacagctt tttgctgctg ttcatgggtg
      661 agtgaaggta aacctgcaaa cattgttaac tcctgctaaa ttgttggcgc taattatttc
      721 atgctacccg gcacatagcc agtagagtca ggactgatga agacgttatc tcccgctgtg
      781 attactttac cctggcgtca ggacgccgct gaattttatt tctcccgctt aagccacctg
      841 ccgtgggcga tgcttttaca ctccggctat gccgatcatc cgtatagccg ctttgatatt
      901 gtggtcgccg atccgatttg cactttaacc actttaggta aagaaaccgt tgttagtgaa
      961 agcgaaaaac gcacaacgac cactgatgac ccgctacagg tgctccagca ggtgctggat
     1021 cgcgcagaca ttcgcccaac gcataacgaa gatttgccat ttcagggcgg cgcactgggg
     1081 ttgtttggct acgatctggg ccgccgtttt gagtcactgc cagaaattgc ggaacaagat
     1141 atcgttctgc cggatatggc agtgggtatc tacgattggg cgctcattgt cgaccaccag
     1201 cgtcatacag tttctttgct gagtcataat gatgtcaatg cccgtcgggc ctggctggaa
     1261 agccagcaat tctcgccgca ggaagatttc acgctcactt ccgactggca atccaatatg
     1321 acccacgagc agtacggcga aaaatttcgc caggtacagg aatatctgca cagcggtgat
     1381 tgctatcagg tgaatctcgc ccagcgtttt catgcgacct attctggcga tgaatggcag
     1441 gcattccttc agcttaatca ggccaaccgc gcgccattta gcgctttttt acgtcttgaa
     1501 cagggtgcaa ttttaagcct ttcgccagag cggtttattc tttgtgataa tagtgaaatc
     1561 cagacccgcc cgattaaagg cacgctacca cgcctgcccg atcctcagga agatagcaaa
     1621 caagcagaaa aactggcgaa ctcagcgaaa gatcgtgccg aaaatctgat gattgtcgat
     1681 ttaatgcgta atgatatcgg tcgtgttgcc gtagccggtt cggtaaaagt accagagctc
     1741 ttcgtggtgg aacccttccc tgccgtgcat catctggtca gcactataac ggcgcgacta
     1801 ccagaacagt tacacgccag cgatctgctg cgcgcagctt ttcctggtgg ctcaataacc
     1861 ggggctccga aagtacgggc tatggaaatt atcgacgaac tggaaccgca gcgacgtaat
     1921 gcctggtgcg gcagcattgg ctatttgagc ttttgcggca acatggatac cagcattact
     1981 atccgcacgc tgactgccat taacggacaa atatactgct ctgcgggcgg tggaattgtc
     2041 gccgatagcc aggaagaagc ggaatatcag gaaacttttg ataaagttaa taagatatta
     2101 cgccaactgg agaagtaaaa cgtggaatac cgtagcctga cgcttgatga ttttttatct
     2161 cgctttcaac ttttgcgccc gcaaattaac cgggaaaccc taaatcatcg tcaggctgcc
     2221 gtgttaatcc ccatcgtccg tcgaccacaa ccggggttgt tgctgactca gcgttcaatt
     2281 catctgcgta aacacgctgg acaagtggca ttccctggag gtgcagtcga tgacacggac
     2341 gcatcagtta tcgccgccgc gctgcgcgaa gctgaagaag aggtcgctat accgccttcc
     2401 gccgttgaag ttatcggcgt gctgccgccc gtcgatagcg taactggcta tcaggtaacc
     2461 ccagtggtcg gcattatccc gcccgatctg ccgtatcgcg ccagtgaaga tgaagtctcg
     2521 gcggtgtttg aaatgccgct cgcccaggca ttacatctgg gtcgttatca ccctttagat
     2581 atctaccgcc gtggcgattc acatcgggta tggctgtcct ggtacgaaca gtattttgta
     2641 tggggaatga ccgcaggcat aattcgtgag ctggcgctgc aaattggtgt gaacccctga
     2701 ctatacttat ctttacatct acaaaacact acttgagaca atcatcgcaa tattagttaa
     2761 atcgcggttt ttgattagtt taattcatgt gaatagttaa gccagtcgcc gcgttccctc
     2821 ttacactatg cgctgttatt agttcgttac tggaagtcca gtcaccttgt caggagtatt
     2881 atcgtgatta gtctattcga catgtttaag gtggggattg gtccctcatc ttcccatacc
     2941 gtagggccta tgaaggcggg taaacagttc gtcgataatc tggtcgaaaa aggcttactg
     3001 gataacgtta ctcgcgttgc cgtggacgtt tatggttcac tgtcgctgac gggtaaaggc
     3061 caccacaccg atatcgccat tattatgggt cttgcaggta acgaacctgc caccgtggat
     3121 atcgacagta ttcccggttt tattcgcgac gtaaaagagc gcgaacgtct gctgctggca
     3181 cagggacggc atgaagtgga tttcccgcgc gacaacggga tgcgttttca taacggcaac
     3241 ctgccgctgc atgaaaacgg tatgcaaatc cacgcctata acggcgatga agtcgtctac
     3301 agcaaaactt attattccat cggcggcggt tttatcgtcg atgaagaaca ctttggtcag
     3361 gatgctgcca acgaagtgag cgtgccgtat ccgttcaaat ctgccaccga actgctcgcg
     3421 tactgtaatg aaaccggcta ttcgctgtct ggtctcgcta tgcagaacga actggcgctg
     3481 cacagcaaga aagagatcga cgagtatttc gcgcatgtct ggcaaaccat gcaggcatgt
     3541 atcgatcgcg ggatgaacac cgaaggtgta ctgccaggcc cgctgcgcgt gccacgtcgt
     3601 gcgtctgccc tgcgccggat gttagtttcc agcgataaac tgtctaacga tccgatgaat
     3661 gtcattgact gggtaaacat gtttgcgctg gcagttaacg aagaaaacgc cgccggtggt
     3721 cgtgtggtaa ctgcgccaac caacggtgcc tgcggtatcg ttccggcagt gctggcttac
     3781 tatgaccact ttattgaatc ggtcagccca gacatctata cccgttactt tatggcagcg
     3841 ggcgcgattg gtgcattgta taaaatgaac gcctctattt ccggtgcgga agttggttgc
     3901 cagggcgaag tgggtgttgc ctgttcaatg gctgctgcgg gtcttgcaga actgctgggc
     3961 ggtagcccgg aacaggtttg cgtggcggcg gaaattggca tggaacacaa ccttggttta
     4021 acctgcgacc cggttgcagg gcaggttcag gtgccgtgca ttgagcgtaa tgccattgcc
     4081 tctgtgaagg cgattaacgc cgcacggatg gctctgcgcc gcaccagtgc accgcgcgtc
     4141 tcgctggata aggtcatcga aacgatgtac gaaaccggta aggacatgaa cgccaaatac
     4201 cgcgaaacct cacgcggtgg tctggcaatc aaagtccagt gtgactaata cttcttactc
     4261 gcccatctgc aacggatggg cgaatttata cccgctttct cgtctgctgt aatattcccc
     4321 actacacttc cactgttgcg tcaggcgttt gtcgccatac gcttacaggg tggcccgcat
     4381 gcaaaaagca cagcggatca ttaaaaccta tcgccgtaat cgaatgattg tttgtacgat
     4441 ttgcgcactc gttacgctcg cttcgaccct gagcgtgcga tttatttcac agcgtaactt
     4501 aaatcaacaa cgggtagtac aattcgccaa tcacgctgta gaggaattag ataaagtact
     4561 gcttccccta caggcaggta gcgaagtctt gcttccgctg attggtctgc cctgctctgt
     4621 cgcccatttg ccattacgta aacaggcggc aaaactccaa actgtgcgat ccattggcct
     4681 ggtgcaagac ggcacacttt attgctccag catttttggt tatcgcaatg tgcccgtcgt
     4741 ggacattctg gctgaacttc ctgcaccgca accactttta cgcctgacga tcgaccgtgc
     4801 cctgattaaa ggcagtccgg ttttgattca atggacgcct gcagcaggca gtagcaatgc
     4861 tggggtcata gagatgatta acatcgactt actgacggca atgctccttg agccacaact
     4921 gccgcaaatc agtagcgcca gcctgacggt ggacaaacgg catttgctct atggtaatgg
     4981 gctggtagat tcccttccgc aacctgaaga caatgaaaac tatcaggttt cttcgcaaag
     5041 ctttcctttt accattaacg taaatggtcc gggggctacg gcgctggcat ggcactatct
     5101 tccaacacaa ttaccgctgg cggtgctgct aagtttactg gtgggctata tcgcctggct
     5161 ggcgaccgct taccggatga gcttttcccg cgaaattaat ctgggcctgg cgcaacatga
     5221 gttcgaattg ttctgtcagc ctttgcttaa tgcgcgcagc cagcaatgta ttggtgtaga
     5281 gattttgctg cgctggaata atccgcgtca gggctggatt tcaccggatg tgtttattcc
     5341 tatcgcggaa gaacatcatt taattgtgcc actgacccgc tatgtgatgg cagaaaccat
     5401 tcgtcagcgc catgttttcc cgatgagtag tcagtttcat gttggcatta acgtcgcacc
     5461 cagccatttt cgccgtggtg tgctgataaa agatctcaat cagtactggt ttagcgctca
     5521 cccgattcag caactgatcc tcgaaatcac cgaacgcgat gccttactgg atgttgatta
     5581 tcggattgcc cgcgagctgc atcgtaaaaa cgtcaaactg gcgattgatg acttcggcac
     5641 cggcaacagc tcgttttcct ggcttgaaac attacgtcct gacgtgctga aaattgataa
     5701 gtcatttacc gcagctatag gttctgacgc ggttaactcg acggtgaccg atatcatcat
     5761 cgcgctgggg caaagactga atattgaact ggtggcggag ggcgtggaaa cgcaagaaca
     5821 ggcgaagtat ttgcgccgtc atggcgtgca tattttgcaa gggtatttgt acgcacagcc
     5881 gatgccgcta cgtgattttc ccaaatggct ggcgggcagc caaccgccgc ccgcccggca
     5941 taatggacat atcacgccca ttatgtcgtt acgttaacgc ttactcatct tcatcgtgcg
     6001 ccggttgctc tttaacaatg cgaaccagat caacacgata atcattggct tcaaggatgg
     6061 tgatatgcag tggccctaca tcaatcacat cgcccacacg gggaatgtga ccatttgccg
     6121 agatcacgag gcccgcgacc gtcgcgatat catcgtcatc ggcaaggtgc tcaacatcaa
     6181 gcgcctgctg caaggcatgc aaatctgtac cgccttttac cagccagccg ttaccatcgg
     6241 taatgatttc cggcgtttcg tcagcgtccg ggaattcacc cgcaatggct tccagcacat
     6301 ccagcggcgt gaccaagcct tgtaccacac caaactcgtt ggtcacgata acaaagctcc
     6361 cgcgagcgcg acgcagcacg cccagcaggt tgatcggatc gagggtttcc gggacgataa
     6421 tcgccggaga cgccgaagca atcgccgcca catcaacgcc ctcttccagc gccaccagca
     6481 gttctttagc acgtacgata ccgatgattt catccagttc accgcgacat accgggaaca
     6541 gactgtgcgg tgaagagagc agttgctcgc ggatttcatc gaccccgaga ttagcgtcaa
     6601 cccagcttat ttcaccgcgc ggcgtcatga tcccgcgcag agaacgcgac gccagcgtca
     6661 gtacgccgtt aatcatgtac cgttcttctt cggcaaatgc accttccggg atcggcatcg
     6721 ggttatcggc atcgtgctga acattggcct gacgtttccc gcccatcaaa cgcaggatgg
     6781 catcggcagt acgcgctcgc agcggcaaag tcgactggtg acgaataaag ttgcgacgcg
     6841 caatctggtt aaacacttcg ataatgatcg agaagccaat cgcggcatac aggtaacctt
     6901 tcggaatgtg gaaaccgaaa ccttctgcca ccagactcag accaatcatt aacaggaagc
     6961 tcagacagag caccaccacc gtggggtgct ggttaacgaa tcgcgtcagc ggtttcgatg
     7021 ccagcaacat aaccgccatc gcaatcacta ccgccgccat catcaccggc agatggttaa
     7081 ccatccctac tgcagtaatt accgcatcca acgagaagac ggcgtcaagg atgacaatct
     7141 gtgtgacgac cacccagaaa ctggcgtagc ctttaccgtg gccggaatca tgatcgcagt
     7201 tttccagccg ttcatgcagt tcggttgttg ctttgaacag caagaatatc cccccgaaca
     7261 acataatcag atcgcgtccg gagaaggaga aatccatgac ggtaaatagc ggtttggtca
     7321 gcgtgaccat ccatgaaatc agcgacagca gccccagacg cataatcagc gccagtgata
     7381 accccagcaa acgcgcttta tcgcgttgtt ttggcggcag tttgtcagca agaatggcga
     7441 tgaagaccag gttatcgata cccagcacaa tttcgagaac aacaagcgtg agtagccccg
     7501 cccaaattga ggggtccatt aagaattcca tgacaagctc ctgcttaagg aatagctatt
     7561 cgacgccaga aataatgcag gcgtaacgac aaaatgcaaa cgaaaggtgc ggcatagagt
     7621 gccagaaagg caggcgttaa aaggcctgat gctgaaatga cgtcggtgac gatccatact
     7681 gcgggctact gccctatact ccatggttgt taaacgggag ttaaacatat cagagacgcc
     7741 tctgatttgg caaagattta ccttcctttg caaacgaatg tgacaaggat attttacctt
     7801 tcgaaatttc tgataatcga aagttaaatt acggatcttc atcacataaa ataatttttt
     7861 cgatatctaa aataaatcgc gaaacgcagg ggtttttggt tgtagccctt atctgaatcg
     7921 attcgattgt ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt aacgataata
     7981 aaggaggtag caagtgacca ttgctattgt tataggcaca catggttggg ctgcagagca
     8041 gttgcttaaa acggcagaaa tgctgttagg cgagcaggaa aacgtcggct ggatcgattt
     8101 cattccaggt gaaaatgccg aaacgctgat tgaaaaatac aacgctcagt tggcaaaact
     8161 cgacaccact aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc
     8221 tgccagccgc attgtcgtcg acaaagagca ttatgaagtc attgcaggcg ttaacattcc
     8281 aatgctcgtg gaaacgttaa tggcccgtga tgatgacccg agctttgatg aactggttgc
     8341 gctggcagta gaaacaggcc gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa
     8401 agccgcgcca gcacccgctg ccgcagcacc aaaagcggct ccaactccgg caaaaccaat
     8461 ggggccaaac gactacatgg ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg
     8521 tcaggtcgcc acccgctgga ccaaagaaac caatgtctcc cgtattattg ttgttagtga
     8581 tgaagtggct gcggataccg ttcgtaagac actgctcacc caggttgcac ctccgggcgt
     8641 aacagcacac gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc
     8701 tggcgaacgc gtaatgctgt tatttaccaa cccaacagat gtagagcgtc tcgttgaagg
     8761 cggcgtgaaa atcacctctg ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca
     8821 ggtgaataac gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaaa aactgaatgc
     8881 acgcggtatt gagctggaag tccgtaaggt ttccaccgat ccgaaactga aaatgatgga
     8941 tctgatcagc aaaatcgata agtaacgtat tgtgttgatt atcattcagt tttcacactt
     9001 aagtcttacg taaacaggag aagtacaatg gagattacca ctcttcaaat tgtgctggta
     9061 tttatcgtag cctgtatcgc aggtatggga tcaatcctcg atgaatttca gtttcaccgt
     9121 ccgctaatcg cgtgtaccct ggtgggtatc gttcttgggg atatgaaaac cggtattatt
     9181 atcggtggta cgctggaaat gatcgcgctg ggctggatga acatcggtgc tgcagttgcg
     9241 cctgacgccg ctctggcttc tatcatttct accattctgg ttatcgcagg tcatcagagc
     9301 attggtgcag gtatcgcact ggcaatccct ctggccgctg cgggccaggt actgaccatc
     9361 atcgttcgta ctattaccgt tgctttccag cacgctgcgg ataaggctgc tgataacggc
     9421 aacctgacag cgatttcctg gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg
     9481 gctattccgg ccgtcatcgt tgcgctgtct gttggtacca gcgaagtaca gaacatgctg
     9541 aatgcgattc cggaagtggt gaccaatggt ctgaatatcg ccgggggcat gatcgtggtg
     9601 gttggttatg cgatggttat caacatgatg cgtgctggct acctgatgcc gttcttctac
     9661 ctcggcttcg taaccgcagc attcaccaac tttaacctgg ttgctctggg tgtgattggt
     9721 actgttatgg cagtgctcta catccaactt agcccgaaat acaaccgcgt agccggtgcg
     9781 cctgctcagg cagctggtaa caacgatctc gataacgaac tggactaaca ggtgagcgaa
     9841 atggttgata caactcaaac taccaccgag aaaaaactca ctcaaagtga tattcgtggc
     9901 gtattcctgc gttctaacct cttccagggt tcatggaact tcgaacgtat gcaggcactg
     9961 ggtttctgct tctctatggt accggcaatt cgtcgcctct accctgagaa caacgaagct
    10021 cgtaaacaag ctattcgccg tcacctggag ttctttaaca cccagccgtt cgtggctgca
    10081 ccgattctcg gcgtaaccct ggcgctggaa gaacagcgtg ctaatggcgc agagatcgat
    10141 gacggtgcta tcaacggtat caaagtcggt ttgatggggc cgctggctgg tgtaggcgac
    10201 ccgatcttct ggggaaccgt acgtccggta tttgcagcac tgggtgccgg tatcgcgatg
    10261 agcggcagcc tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt gcgtctggca
    10321 acccgttact acggcgtagc ttatggttac tccaaaggta tcgatatcgt taaagatatg
    10381 ggtggtggct tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct gtttgtcatg
    10441 ggggcattgg ttaacaagtg gacacatgtc aacatcccgc tggtggtttc gaccatcacc
    10501 gggcaggatg gacagacccg tgtcaccacc gtgcaaacta ttcttgacca gttaatgcca
    10561 ggccttgtac cactgctgct gacatttgct tgtatgtggc tgctgcgcaa aaaagttaac
    10621 ccgctgtgga tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg
    10681 ctgggactgt aagactgttg tacactaccg gggccttttg gccccgtttt tttatctgga
    10741 ggattaatga caatcacgga cctggtactg attcttttca tcgccgcact cctggccttc
    10801 gcgatctacg atcagttcat catgccccgc cgtaacggcc ccaccctgct ggcaattcct
    10861 ttgctccgcc gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt cattcttatc
    10921 tataacaacg tcacgaatca tggcgcgcta ataacaacat ggttattaag tgcactggct
    10981 ctgatgggtt tttatatatt ctggatccgc attccgaaga tcatctttaa acaaaaaggt
    11041 tttttcttcg ccaatgtctg gattgaatat agccgaatca aagcgatgaa cttgtcggaa
    11101 gatggcgtgt tggtgatgca attagaacag cgtcggctgt taatccgcgt tcgaaatatc
    11161 gacgatctgg aaaaaattta taagcttctc gta
//
LOCUS       DAJTYI010000122        11099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYI010000122 DAJTYI010000000
VERSION     DAJTYI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..740)
                     /locus_tag="OO564_003185"
     CDS             complement(<1..740)
                     /locus_tag="OO564_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR6654215.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEA"
     gene            1019..1972
                     /gene="tal"
                     /locus_tag="OO564_003186"
     CDS             1019..1972
                     /gene="tal"
                     /locus_tag="OO564_003186"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6654216.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2087..2674
                     /gene="mog"
                     /locus_tag="OO564_003187"
     CDS             2087..2674
                     /gene="mog"
                     /locus_tag="OO564_003187"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6654217.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2709..3275)
                     /gene="satP"
                     /locus_tag="OO564_003188"
     CDS             complement(2709..3275)
                     /gene="satP"
                     /locus_tag="OO564_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6654218.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3424..4137)
                     /gene="msyB"
                     /locus_tag="OO564_003189"
     CDS             complement(3424..4137)
                     /gene="msyB"
                     /locus_tag="OO564_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6654219.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4163..4567)
                     /locus_tag="OO564_003190"
     CDS             complement(4163..4567)
                     /locus_tag="OO564_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6654220.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4944..6860
                     /gene="dnaK"
                     /locus_tag="OO564_003191"
     CDS             4944..6860
                     /gene="dnaK"
                     /locus_tag="OO564_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6654221.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6949..8079
                     /gene="dnaJ"
                     /locus_tag="OO564_003192"
     CDS             6949..8079
                     /gene="dnaJ"
                     /locus_tag="OO564_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6654222.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8183..8335)
                     /gene="mokC"
                     /locus_tag="OO564_003193"
     CDS             complement(8183..8335)
                     /gene="mokC"
                     /locus_tag="OO564_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6654223.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            8921..10087
                     /gene="nhaA"
                     /locus_tag="OO564_003194"
     CDS             8921..10087
                     /gene="nhaA"
                     /locus_tag="OO564_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6654224.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            10153..11052
                     /gene="nhaR"
                     /locus_tag="OO564_003195"
     CDS             10153..11052
                     /gene="nhaR"
                     /locus_tag="OO564_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6654225.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
BASE COUNT         2846 a         2703 c         2883 g         2667 t
ORIGIN      
        1 gcttcctgcc agccaaaagc actctcaaaa atagaccaaa tgacgtgggg aagttgcccg
       61 atatttatta cgcaaatcac caggctggtc agtacccaga ttatcgccat caacgggaca
      121 aacccctgca tgagccgggc gacgccatga agaccgcgag tgattgccag cagagcaaag
      181 acagcgagaa taatgcccgt caccagcgga ggaaaatcaa aagaaaaact cagggctcgg
      241 gcaacggcgt tcgcctgaac tccgctgaaa attatgccgt aggcgatgag caaaaagacg
      301 gcgaacagaa cgcccatcca gcgcatcccc agcccgcgcg ccatatacca tgccggtccg
      361 ccacgaaact gcccattgac gtcacgttct ttataaagtt gtgcaaggga acattcggca
      421 aacgaggtcg ccatgccgat aaacgcagca acccacatcc aaaagacggc acctggtcca
      481 ccggcggtaa ttgccagcgc aacgccggcc aggttgccgc tacccacgcg cgccgcaaga
      541 ctggtacaca atgactgaaa tgaggttaaa ccgcctggct gtggatgaat gctattttta
      601 agacttttgc caaactggcg gatgtagcga aactgcacaa atccggtgcg aaaagtgaac
      661 caacaacctg cgccgaagag caggtaaatc attaccgatc cccaaaggac gctgttaata
      721 aaggagaaaa aatctggcat gcatatccct cttattgccg gtcgcgatga ctttcctgtg
      781 taaacgttac caattgttta agaagtatat acgctacgag gtacttgata acttctgcgt
      841 agcatacatg aggttttgta taaaaatggc gggcgatatc aacgcagtgt cagaaatccg
      901 aaacagtctc gcctggcgat aaccgtcttg tcggcggttg cgctgacgtt gcgtcgtgat
      961 atcatcaggg cagaccggtt acatccccct aacaagctgt ttaaagagaa atactatcat
     1021 gacggacaaa ttgacctccc ttcgtcagta caccaccgta gtggccgaca ctggggacat
     1081 cgcggcaatg aagctgtatc aaccgcagga tgccacaacc aacccttctc tcattcttaa
     1141 cgcagcgcag attccggaat accgtaagtt gattgatgat gctgtcgcct gggcgaaaca
     1201 gcagagcaac gatcgcgcgc agcagatcgt ggacgcgacc gacaaactgg cagtaaatat
     1261 tggtctggaa atcctgaaac tggttccggg ccgtatctca actgaagttg atgcgcgtct
     1321 ttcctatgac accgaagcgt caattgcgaa agcaaaacgc ctgatcaaac tctacaacga
     1381 tgcaggtatt agcaacgatc gtattctgat caaactggct tctacctggc agggtatccg
     1441 tgctgcagaa cagctggaaa aagaaggtat caactgtaac ctgaccctgc tgttctcctt
     1501 cgctcaggct cgtgcttgtg cggaggcggg cgtgttcctg atctcgccgt ttgttggccg
     1561 tattcttgac tggtacaaag cgaataccga taagaaagag tacgctccgg cagaagatcc
     1621 gggcgtggtt tctgtatctg aaatctacca gtactacaaa gagcacggtt atgaaaccgt
     1681 ggttatgggc gcaagcttcc gtaacatcgg cgaaattctg gaactggcag gctgtgaccg
     1741 tctgaccatc gcaccggcac tgctgaaaga gctggcggag agcgaagggg ctatcgaacg
     1801 taaactgtct tacaccggcg aagtgaaagc gcgtccggcg cgtatcactg agtccgagtt
     1861 cctgtggcag cacaaccagg atccaatggc agtagataaa ctggcggaag gtatccgtaa
     1921 gtttgctatt gaccaggaaa aactggaaaa aatgatcggc gatctgctgt aatcattctt
     1981 agcgtgaccg ggaagtcggt cacgctacct cttctgaagc ctgtctgtca ctcccttcgc
     2041 agtgtatcat tctgtttaac gagactgttt aaacggaaaa atcttgatga atactttacg
     2101 tattggctta gtttccatct ctgatcgcgc atccagcggc gtttatcagg ataaaggcat
     2161 tccagcgctg gaagaatggc tgacatcggc gctaaccacg ccgtttgaac tggaaacccg
     2221 cttaatcccc gatgagcagg cgatcatcga gcaaacgttg tgtgagctgg tggatgaaat
     2281 gagttgccat ctggtgctca ccacgggcgg aactggcccg gcgcgtcgtg acgtaacgcc
     2341 cgatgcgacg ctggcagtag cggaccgcga gatgcctggc tttggtgaac agatgcgcca
     2401 gatcagcctg cattttgtac caactgcgat cctttcgcgt caggtgggcg tgattcgcaa
     2461 acaggcgctg atccttaact tacccggtca gccgaagtct attaaagaga cgctggaagg
     2521 tgtgaaggac gctgagggta acgttgtggt gcatggtatt tttgccagcg taccgtactg
     2581 cattcagttg ctggaagggc catacgttga aacggcacca gaagtggttg ctgcattcag
     2641 accgaagagt gcaagacgcg acgttagcga ataaaaaaat ccccccgagc ggggggatct
     2701 caagacaatt agtgggattc accaatcggc agaacggtgc gaccaaactg ctcgttcagc
     2761 acttcaccca tcgccagata gattgcgctg gcaccgcaga tcagaccaat ccagccggca
     2821 aagtggatga tcgcggcatt accggcaatg ttaccgatcg ccagcagggc aaacagcacg
     2881 gtcaggctaa agaaaacgaa ttgcaggacg cgtgcgcctt tcagagtgcc gaagaacata
     2941 aacagcgtaa atacgcccca cagacccagg tagacaccaa ggaactgtgc atttggcgca
     3001 tcggtcagac ccagtttcgg catcagcaga atcgcaacca gcgtcagcca gaaagaaccg
     3061 taagaggtga atgcggttaa accgaaagtg ttgccttttt tgtactccag cagaccagca
     3121 aaaatttgcg cgatgccgcc gtagaaaatg cccatggcaa gaataatacc gtccagagcg
     3181 aaataaccca cgttgtgcag gttaagcaga atggtggtca tgccgaagcc catcaggccc
     3241 agcggtgccg gattagccaa cttagtgttg cccataattc ctcaaaaatc atcatcgaat
     3301 gaatggtgaa ataatttccc tgaataactg tagtgttttc agggcgcggc ataataatca
     3361 gccagtgggg cagtgtctac gatcttttga ggggaaaatg aaaattttcc ccggtttccg
     3421 gtatcagacc tgagtggcgc taaccatccg gcgcaggcag gcgatttgca gtacggctgg
     3481 aatcgtcacg cgataggcgc tgccgctgac cgctttaacc ccatttaatg ctgcgcccac
     3541 agggcctccc agccccgctc cgcgcagcaa accatgccca agtacgctca ttgctgcgtg
     3601 ggtgcgtaaa atgcgggtca gttggctgga aagcaaatgc gacacacctt ttgccaataa
     3661 tttgtctttc atcagcagcg gcagcagctc ttccagctca ttcaccctgg catcgaccgc
     3721 gtgcagaaac tcctgcttat gttcctcgtc cattttcttc caggtattac gcagaaattg
     3781 ttccagtaac tgttgctcaa tttcaaacgt agacatctct ttgtcggctt tcagcttcaa
     3841 tcgctttgaa acatcgagca aaatggcccg atacaattta ccgtgtccgc gcagtttgtt
     3901 ggcgatacta tcgccaccaa aatgctgtaa ttctccggca atcagctgcc agttgcggcg
     3961 atgttgctcg ggatgccctt ccatcgattt aaacagttcg ttgcgcatca gtacgctgga
     4021 gaggcgagtt ttgccttttt cattatgggt gagcaatcgg gcgaaatttg ccaactgttc
     4081 ctcactacaa tgctgaagaa aatccagatc tgaatcattc aggtaattaa cattcatttt
     4141 ttgtggcttc tatattctgg cgttagtcgt cgccgataat tttcagcgtg gccatatccg
     4201 atgagttcac cgtatgaccg gaaaaggtga tttttgagac gcagcgttta ttgtcgttat
     4261 cgctgttaat gttgatccag tcagtggttt gcccttcttt tatttctgaa ggaatattca
     4321 ggctctgact ggcgctacgg gcggctttga aataaaccga tgcaccgctt aactgtaaat
     4381 cgccatggtc ggcagagagt tgtatgcgtt tcacaatgcg acaaacagga agtttcagcg
     4441 ccagatcgtt ggtttcgtta cgcggcattg caatgacgcc gaggagttta tggtcgtttg
     4501 cctgcgccgt gcagcacagc atcaggctaa tcgccaggct ggcggaaatc gtaaaaacgg
     4561 atttcataag gattctctta gtgggaagag gtagggggat gaatacccac tagtttactg
     4621 ctgataaaga gaagattcag gcacgtaatc ttttcttttt attacaattt tttgatgaat
     4681 gccttggctg cgattcattc tttatatgaa taaaattgct gtcaatttta cgtcttgtcc
     4741 tgccatatcg cgaaatttct gcgcaaaagc acaaaaaatt tttgcatctc ccccttgatg
     4801 acgtgggtta cgaccccatt tagtagtcaa ccgcagtgag tgagtctgca aaaaaatgaa
     4861 attgggcagt tgaaaccaga cgtttcgccc ctattacaga ctcacaacca catgatgacc
     4921 gaatatatag tggagacgtt tagatgggta aaataattgg tatcgacctg ggtactacca
     4981 actcttgtgt agcgattatg gatggcacca ttcctcgcgt gctggagaac gccgaaggcg
     5041 atcgcaccac gccttctatc attgcctata cccaggatgg tgaaactctg gttggtcagc
     5101 cggctaaacg tcaggcagtg acgaacccgc aaaacaccct gtttgcgatt aaacgcctga
     5161 ttggccgccg cttccaggac gaagaagtac agcgtgatgt ttccatcatg ccgttcaaaa
     5221 ttattgctgc tgataacggc gacgcatggg tcgaagttaa aggccagaaa atggcaccgc
     5281 cgcagatttc tgctgaagtg ctgaaaaaaa tgaagaaaac cgctgaagat tacctgggtg
     5341 aaccggtaac tgaagctgtt atcaccgtac cggcatactt taacgatgct cagcgtcagg
     5401 caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa acgtatcatc aacgaaccga
     5461 ccgcagctgc gctggcttac ggtctggaca aaggcactgg caaccgtact atcgcggttt
     5521 atgacctggg gggtggtact ttcgatattt ctattatcga aatcgacgaa gttgacggcg
     5581 aaaaaacctt cgaagttctg gcaaccaacg gtgataccca cctgggtggt gaagacttcg
     5641 acagccgtct gatcaactat ctggttgaag aattcaagaa agatcagggc attgacctgc
     5701 gcaacgatcc gctggcaatg cagcgcctga aagaagcggc agaaaaagcg aaaatcgaac
     5761 tgtcttccgc tcagcagacc gacgttaacc tgccatacat cactgcagac gcgaccggtc
     5821 cgaaacacat gaacatcaaa gtgactcgtg cgaaactgga aagcctggtt gaagatctgg
     5881 taaaccgttc cattgagccg ctgaaagttg cactgcagga tgctggcctg tccgtatctg
     5941 atatcgacga cgttatcctc gttggtggtc agactcgtat gccaatggtt cagaagaaag
     6001 ttgctgagtt ctttggtaaa gagccgcgta aagacgttaa cccggacgaa gctgtagcaa
     6061 tcggtgctgc tgttcagggt ggtgttctga ctggtgacgt aaaagacgta ctgctgctgg
     6121 acgttacccc gctgtctctg ggtatcgaaa ccatgggcgg tgtgatgacg acgctgatcg
     6181 cgaaaaacac cactatcccg accaagcaca gccaggtgtt ctctaccgct gaagacaacc
     6241 agtctgcggt aaccatccat gtgctgcagg gtgaacgtaa acgtgcggct gataacaaat
     6301 ctctgggtca gttcaaccta gatggtatca acccggcacc gcgcggcatg ccgcagatcg
     6361 aagttacctt cgatatcgat gctgacggta tcctgcacgt ttccgcgaaa gataaaaaca
     6421 gcggtaaaga gcagaagatc accatcaagg cttcttctgg tctgaacgaa gatgaaatcc
     6481 agaaaatggt acgcgacgca gaagctaacg ccgaagctga ccgtaagttt gaagagctgg
     6541 tacagactcg caaccagggc gaccatctgc tgcacagctc ccgtaagcag gttgaagaag
     6601 caggcgacaa actgccggct gacgacaaaa ctgctatcga gtctgcgctg actgcactgg
     6661 aaactgctct gaaaggtgaa gacaaagccg ctatcgaagc gaaaatgcag gaactggcac
     6721 aggtttccca gaaactgatg gaaatcgccc agcagcaaca tgcccagcag cagactgccg
     6781 gtgctgatgc ttctgcaaac aacgcgaaag atgacgatgt tgtcgacgct gaatttgaag
     6841 aagtcaaaga caaaaaataa tcgccttata aacgggtaat tatactgaca cgggcgaagg
     6901 ggaatttcct ctccgcccgt gcattcatct aggggcaatt taaaaaagat ggctaagcaa
     6961 gattattacg agattttagg cgtttccaaa acagcggaag agcgtgaaat cagaaaggcc
     7021 tacaaacgcc tggccatgaa ataccacccg gaccgtaacc agggtgacaa agaggccgag
     7081 gcgaaattta aagagatcaa ggaagcttat gaagttctga ccgactcgca aaaacgtgcg
     7141 gcatacgatc agtatggtca tgctgcgttt gagcaaggag gcatgggcgg cggtggtttt
     7201 ggcggcggcg cagacttcag cgatattttt ggtgacgttt tcggcgatat ttttggcggc
     7261 ggacgtggtc gtcaacgtgc ggcgcgcggt gctgatttac gctataacat ggagctcacc
     7321 ctcgaagaag ctgtacgtgg cgtgaccaaa gaaatccgca ttccgactct ggaagagtgt
     7381 gacgtttgcc acggtagcgg tgcaaaacca ggtacacagc cgcagacctg tccgacctgt
     7441 catggttctg gtcaggtgca gatgcgccag ggattcttcg ctgtacagca gacctgtcca
     7501 cactgtcagg gccgcggtac gctgatcaaa gatccgtgca acaaatgtca tggtcatggt
     7561 cgtgttgagc gcagcaaaac gctgtccgtt aaaatcccgg caggggtgga cactggagac
     7621 cgcatccgtc ttgcgggcga aggtgaagcg ggcgagcatg gcgcaccggc aggcgatctg
     7681 tacgttcagg ttcaggttaa acagcacccg attttcgagc gtgaaggcaa caacctgtat
     7741 tgcgaagtcc cgatcaactt cgctatggcg gcgctgggtg gcgaaatcga agtaccgacc
     7801 cttgatggtc gcgtcaaact gaaagtgcct ggcgaaaccc agaccggtaa gctattccgt
     7861 atgcgcggta aaggcgtcaa gtctgtccgc ggtggtgcac agggtgattt gctgtgccgc
     7921 gttgtcgtcg aaacaccggt aggcctgaac gaaaagcaga aacagctgct gcaagagctg
     7981 caagaaagct tcggtggccc aaccggcgag cacaacagcc cgcgctcaaa gagcttcttt
     8041 gatggtgtga agaagttttt tgacgacctg acccgctaac ctccccaaaa gcctgcccgt
     8101 gggcaggcct gggtaaaaat agggtgcgtt gaagatatgc gagcacctgt aaagtggcgg
     8161 ggatcactcc ccgccgttgc tcttactcgg attcgtaagc cgtgaaaaca gcaacctccg
     8221 tctggccagt tcggatgtga acctcacaga ggtcttttct cgtcaccagc gccgccacta
     8281 cggcggtgat acagatgacg atcagggcga caatcatcgc cttatgctgc ttcattgctc
     8341 tcttctcctt gaccttacgg tcagtaagag gcactctaca tgtgttcagc atataggagg
     8401 cctcgggttg atggtaaaat atcactcggg gcttttctct atctgccgtt cagctaatgc
     8461 ctgagacaga cagcctcaag cacccgccgc tattatatcg ctctctttaa cccattttgt
     8521 tttatcgatt ctaatcctga agacgcctcg catttttgtg gcgtaatttt ttaatgattt
     8581 aattatttaa ctttaattta tctcttcatc gcaattattg acgacaagct ggattatttt
     8641 tgaaatattg gcctaacaag catcgccgac tgacaacaaa ttaattatta cttttcctaa
     8701 ttaatccctc aggaatcctc accttaagct atgattatct gggcttaggg tcactcgtga
     8761 gcgcttacag ccgtcaaaaa cgcatctcac cgctgatggc gcaaattctt caatagctcg
     8821 taaaaaacga attattccta cactataatc tgattttaac gatgattcgt gcggggtaaa
     8881 atagtaaaaa cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcaatt
     8941 ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat
     9001 tatggccaac agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca
     9061 gctccgggtt ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct
     9121 gatggcggta tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc
     9181 gctagccagc ttacgccagg ccgcatttcc agttatcgcc gctattggtg ggatgattgt
     9241 gccggcatta ctctatctgc cttttaacta tgccgatccg attacccgcg aagggtgggc
     9301 gatcccggcg gctactgaca ttgcttttgc acttggtgta ctggcgctgt tgggaagtcg
     9361 tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg atcttggggc
     9421 catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc
     9481 ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt
     9541 ctatattctt gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac
     9601 tctggcgggg gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc
     9661 agcgaagcga ctggagcatg tgttgcaccc gtgggtggcg tatctgattt tgccgctgtt
     9721 tgcattttct aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat
     9781 tctgccattg gggatcatcg ctggcttgct gattggcaaa ccgctgggga ttagtctgtt
     9841 ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca
     9901 aattatggtg gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag
     9961 cctggccttt ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt
    10021 cggttctatc tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc
    10081 agtttgacag gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg
    10141 agagaaatga gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat
    10201 aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc
    10261 ggacagattc gagcgctgga agagcgcctg caaggcaaat tatttaaacg caagggacgt
    10321 ggtctcgaac ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc
    10381 ttaagccagg aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac
    10441 gttggcgtgg ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa cgccgcagtg
    10501 gtagaaggcg agcccattca tcttcgctgc ttcgaatcca cccacgaaat gctgctggag
    10561 caattaagtc agcataaact ggatatgatc atttctgact gtccgataga ctctacgcag
    10621 caggaaggcc tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat
    10681 ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg
    10741 cgacgttcaa tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta
    10801 gaaatcctcg gcgagtttga tgatgccgct ttgatgaaag cttttggtgc gatgcacaat
    10861 gcaatcttcg ttgccccaac gctttatgca tatgattttt atgccgataa aactgtcgta
    10921 gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg
    10981 attcagcacc cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca
    11041 gcggtgcgtt aatcggcagc tcccccaaag ttaaggtggg ggagatagat tagttgtac
//
LOCUS       DAJTYI010000123        11039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYI010000123 DAJTYI010000000
VERSION     DAJTYI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            317..1957
                     /gene="trg"
                     /locus_tag="OO564_003196"
     CDS             317..1957
                     /gene="trg"
                     /locus_tag="OO564_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6654226.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1995..2918)
                     /locus_tag="OO564_003197"
     CDS             complement(1995..2918)
                     /locus_tag="OO564_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6654227.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3135..4478
                     /locus_tag="OO564_003198"
                     /pseudo
     CDS             3135..4478
                     /locus_tag="OO564_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4703..6358
                     /gene="opgD"
                     /locus_tag="OO564_003199"
                     /gene_synonym="mdoD"
     CDS             4703..6358
                     /gene="opgD"
                     /locus_tag="OO564_003199"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6654228.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6498..6722
                     /locus_tag="OO564_003200"
     CDS             6498..6722
                     /locus_tag="OO564_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6654229.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6785..7321
                     /gene="rimL"
                     /locus_tag="OO564_003201"
     CDS             6785..7321
                     /gene="rimL"
                     /locus_tag="OO564_003201"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6654230.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7316..8296)
                     /locus_tag="OO564_003202"
     CDS             complement(7316..8296)
                     /locus_tag="OO564_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6654231.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8420..9412
                     /gene="tehA"
                     /locus_tag="OO564_003203"
     CDS             8420..9412
                     /gene="tehA"
                     /locus_tag="OO564_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6654232.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9409..10002
                     /gene="tehB"
                     /locus_tag="OO564_003204"
     CDS             9409..10002
                     /gene="tehB"
                     /locus_tag="OO564_003204"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6654233.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10305..10973
                     /locus_tag="OO564_003205"
     CDS             10305..10973
                     /locus_tag="OO564_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6654234.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         2847 a         2580 c         2774 g         2838 t
ORIGIN      
        1 atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc
       61 acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg
      121 tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct
      181 tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg
      241 tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg
      301 acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat
      361 caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag
      421 ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc
      481 ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt cagcccggat
      541 caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa
      601 tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa
      661 tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc aacgctttca
      721 ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc
      781 gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt
      841 gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca
      901 gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac
      961 gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca
     1021 acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggccgtaa
     1081 tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg ggatgacagt
     1141 agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg
     1201 caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc
     1261 cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag
     1321 caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt
     1381 agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt
     1441 catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc
     1501 gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc
     1561 aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt
     1621 aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga
     1681 tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag
     1741 tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa
     1801 tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg
     1861 attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg
     1921 cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca
     1981 gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat caactggcaa
     2041 ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt
     2101 cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa
     2161 gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga agcagacagc
     2221 gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc
     2281 tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc
     2341 gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga
     2401 aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg
     2461 atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact
     2521 tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc
     2581 agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact ttctttacga
     2641 tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat
     2701 tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc
     2761 gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt
     2821 tcagctgcgt gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc
     2881 aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg
     2941 gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat attattaaca
     3001 attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca
     3061 gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat tactgaccac
     3121 tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc
     3181 aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg
     3241 tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa
     3301 atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg
     3361 cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct
     3421 attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg
     3481 atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta
     3541 tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca
     3601 ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac
     3661 aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcactag ttagcaacgg
     3721 tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct
     3781 gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc
     3841 agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc
     3901 gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt
     3961 ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg
     4021 tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg
     4081 ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca
     4141 gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg
     4201 gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg
     4261 gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga
     4321 tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg
     4381 aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaagaag
     4441 cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca
     4501 aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg
     4561 atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag
     4621 cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca
     4681 ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt caatggctat
     4741 ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc
     4801 agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat
     4861 ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct
     4921 ggcgacaatg acgctgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg
     4981 gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg
     5041 tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt
     5101 tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca
     5161 aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga
     5221 tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg
     5281 tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga
     5341 ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc
     5401 gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag
     5461 tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg
     5521 catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac
     5581 aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg
     5641 gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa
     5701 cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat
     5761 gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg
     5821 caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt
     5881 ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta
     5941 ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg
     6001 catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg
     6061 tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga
     6121 gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc
     6181 catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt
     6241 cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca
     6301 gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc
     6361 gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca
     6421 tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc
     6481 actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg
     6541 aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca
     6601 gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa
     6661 agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt
     6721 aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga
     6781 taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag
     6841 tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct
     6901 aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt
     6961 gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat
     7021 cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg
     7081 gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg cattgattca
     7141 tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc
     7201 gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc
     7261 tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata
     7321 ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac
     7381 ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat
     7441 ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt
     7501 cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta
     7561 acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg
     7621 gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat
     7681 cgcatagcca gacgttgttc tcttcattac cttcaacact cgcaaaatca aaaacttcgg
     7741 cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga
     7801 caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg
     7861 gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata
     7921 ttctacattg cccgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac
     7981 cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca
     8041 gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag
     8101 cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc
     8161 agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta
     8221 ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta
     8281 ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg
     8341 cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt
     8401 aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt
     8461 tggtattgtg ttggggacga tagggatggg atttgcttgg cgctatgcca gccaggtttg
     8521 gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt
     8581 attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt
     8641 tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
     8701 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt
     8761 ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga
     8821 ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
     8881 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
     8941 ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc
     9001 cacggcactg cggacatcac tcggcattca gctcgcgcct gcgctggtgg cctgtagtgc
     9061 ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg
     9121 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
     9181 tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
     9241 ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac
     9301 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
     9361 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
     9421 gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtactggaa
     9481 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac
     9541 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
     9601 atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc
     9661 gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg
     9721 ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact
     9781 aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
     9841 gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag
     9901 agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
     9961 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg
    10021 cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt
    10081 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga
    10141 aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa
    10201 ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt
    10261 tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat
    10321 ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca
    10381 cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa gaaacaacct
    10441 cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg
    10501 acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag
    10561 ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag
    10621 cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca
    10681 ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat
    10741 tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt
    10801 ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg
    10861 gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag
    10921 ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga
    10981 cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcaggg
//
LOCUS       DAJTYI010000124        10904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYI010000124 DAJTYI010000000
VERSION     DAJTYI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO564_003206"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO564_003206"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="OO564_003207"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="OO564_003207"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6654235.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="OO564_003208"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="OO564_003208"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6654236.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="OO564_003209"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="OO564_003209"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6654237.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /gene="garP"
                     /locus_tag="OO564_003210"
     CDS             complement(3367..4701)
                     /gene="garP"
                     /locus_tag="OO564_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6654238.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="OO564_003211"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="OO564_003211"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6654239.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="OO564_003212"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="OO564_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6654240.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="OO564_003213"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="OO564_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="OO564_003214"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="OO564_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6654241.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="OO564_003215"
     CDS             9047..9826
                     /locus_tag="OO564_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6654242.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="OO564_003216"
     CDS             9816..10625
                     /locus_tag="OO564_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6654243.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..>10904
                     /locus_tag="OO564_003217"
     CDS             10712..>10904
                     /locus_tag="OO564_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IID with IIC forms the translocation channel;
                     involved in N-acetylgalactosamine transport; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIA"
                     /protein_id="HCR6654244.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFL"
BASE COUNT         2609 a         2929 c         2742 g         2624 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacaaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaataac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggtgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ctaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg ctcgcgccac gctggaagcc catcgtcacg cttttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctga
//
LOCUS       DAJTYI010000125        10744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYI010000125 DAJTYI010000000
VERSION     DAJTYI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            20..1717
                     /gene="maeA"
                     /locus_tag="OO564_003218"
     CDS             20..1717
                     /gene="maeA"
                     /locus_tag="OO564_003218"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6654245.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1851..2861
                     /gene="adhP"
                     /locus_tag="OO564_003219"
     CDS             1851..2861
                     /gene="adhP"
                     /locus_tag="OO564_003219"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6654246.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3007..3291
                     /locus_tag="OO564_003220"
     CDS             3007..3291
                     /locus_tag="OO564_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6654247.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3519..4172)
                     /gene="fdnI"
                     /locus_tag="OO564_003221"
     CDS             complement(3519..4172)
                     /gene="fdnI"
                     /locus_tag="OO564_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6654248.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4165..5049)
                     /gene="fdnH"
                     /locus_tag="OO564_003222"
     CDS             complement(4165..5049)
                     /gene="fdnH"
                     /locus_tag="OO564_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6654249.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5062..8109)
                     /gene="fdnG"
                     /locus_tag="OO564_003223"
     CDS             complement(5062..8109)
                     /gene="fdnG"
                     /locus_tag="OO564_003223"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7522..7524),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6654250.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8341..9222
                     /gene="yddG"
                     /locus_tag="OO564_003224"
     CDS             8341..9222
                     /gene="yddG"
                     /locus_tag="OO564_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6654251.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9481..10581
                     /gene="ompC"
                     /locus_tag="OO564_003225"
     CDS             9481..10581
                     /gene="ompC"
                     /locus_tag="OO564_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6654252.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2634 a         2762 c         2656 g         2692 t
ORIGIN      
        1 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
       61 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
      121 ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga
      181 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
      241 catctacctg cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa
      301 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
      361 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa
      421 tatggacgat attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga
      481 cggtgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
      541 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
      601 gctggatgtc ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg
      661 taatccgcgt atcactgacg atgaatacta tgaattcgtt gatgaattta tccaggctgt
      721 gaaacaacgc tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat
      781 gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac
      841 tgcggcggta acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag
      901 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
      961 catcgcccag acccagcgcg aaggattaag cgaggaagca gcgcggcaga aagtctttat
     1021 ggtcgatcgc tttggcctgc tgacggacaa gatgccgaac ctgctgcctt tccagaccaa
     1081 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact
     1141 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
     1201 gctgtttacg gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat
     1261 gccgctgtct aacccgacgt ctcgcgtgga agccacaccg caggacatta tcgcctggac
     1321 cgaaggtaac gcgctggtcg ccactggcag cccgtttaat cctgtggtat ggaaagataa
     1381 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccgggcatcg gcctgggtgt
     1441 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacact
     1501 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
     1561 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
     1621 cgtggcggtc aaaacgtctg ctgaagcttt gcaacaagcc attgacgata atttctggca
     1681 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcacccggt agtgaaggct
     1741 accgggctat ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
     1801 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaagactg
     1861 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
     1921 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta
     1981 agaatggcga ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg
     2041 tggcagaagt tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
     2101 ggttttacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
     2161 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
     2221 tcaccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcgccc agcagcatta
     2281 cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt
     2341 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg
     2401 tctttaacgc caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
     2461 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
     2521 agaaaaccgg tggcgctcac gctgcggtgg taacagcagt agctaaagct gcgtttaact
     2581 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt
     2641 ctatgagcct ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg
     2701 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
     2761 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
     2821 gcaaaatccg tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc
     2881 ccatgttcgg gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt
     2941 tgcgtaggaa ggaacaataa tgatagtgtt cccagagaag taacgctact gagagtaaag
     3001 acactaatga aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac
     3061 gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca
     3121 agccgattgc tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg
     3181 gtgatcggca gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag
     3241 gcagaaaaaa cggtggttgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca
     3301 ccttataaag cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt
     3361 atcaggccta cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg
     3421 cattcacgac gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc
     3481 ttttacagcg ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct
     3541 tttttcgctt ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag
     3601 cgacggctca ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata
     3661 tacatatgga tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg
     3721 ctgtagcgaa caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc
     3781 ccggtcacca gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg
     3841 gcgttgtact taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg
     3901 ttcaacagcc acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac
     3961 ataaacatca gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc
     4021 tgcggcgtac cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg
     4081 gacagcgcca ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat
     4141 ttggtgcgca caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc
     4201 cttattcggg ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc
     4261 agccgctgcc agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg
     4321 atctttcggc agaccgtgat acagctccgg ctgatcggcg tgatgcagca cgtacataac
     4381 gtgcgtacca ccgacccctt ccgggttgta gacgccagca tgttcgtaac cacgcgcttt
     4441 cagtttcgcc acgcgctgtt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat
     4501 agccccggtc ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac
     4561 gcagagcgtg catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg
     4621 acacccggca atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc
     4681 gttagcgtac tgaatgattg caccagcaga cgggcacgcc ttcaggcagc ccggatcttc
     4741 acagtgcata cagccgtctt tacggatcag ccactccagc ttgccattct gttcggtttc
     4801 gctaaagcgc atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacacc
     4861 gacgcagtgc cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc
     4921 tttacagccg atacaggtgg aaacgtcgat aagtttcgcg acttctgctt tgtaatcacg
     4981 cacctgagaa ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc
     5041 catagccatc tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc
     5101 cggcgtttgc gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt
     5161 acgcgcgaca ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc
     5221 gtttacattc agcggtttca gacggcgcgt taccactgcc accgcgcgga taaagcctcg
     5281 cttgctggag acagtgacac gatcgccatt attaatgcct ttcgccgccg cctgcgtttc
     5341 gctgatttcc acaaactgtt ccggctgagc aattgcgttg agcaacgcgt gcttggtcca
     5401 ggtgtggaag tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt
     5461 acccatccgc agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg
     5521 atgcagcggg ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc
     5581 ttccgccatt ttgttgatgg caaacaggcg tcccatccct tccggctgca taataaacgg
     5641 cccggttggc gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt
     5701 gctgccgttc cactggatca gcatccgttt cggatcccac ggtttaccgt tgatatccgc
     5761 cgaagcacgg ttgtagagta cgcgacggtt gagcggccac gcccaggccc atcccagcgt
     5821 attccccaga ccggacgggt cggagttatc gcggtttgcc atctggttac cctgctctgt
     5881 ccagctaccg gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc
     5941 aaagctactc agcaactgac ctttcttcgc aatcagcacg ccattagcat catagagatc
     6001 ttccaacgca tagccgttgt tctctttagc tacttcgtca gattgcggtt cgtgcggctg
     6061 cttgtagttc cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta
     6121 caactcgcgc agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc
     6181 cggcgcgtcc tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc
     6241 atcctcttca gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg
     6301 atcgacatcg ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg
     6361 atcgataacc accatgtact tcagtttgct caggcagctc accactttgt ttttatccgg
     6421 gaaggacgca accgggttaa agccctggca gaaataaccg gtgactttgc cttcatccat
     6481 catgttgaaa tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc
     6541 ccagttgttc tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa
     6601 cttcggatag ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc
     6661 ttccagatac gactgcaaat caacctgttt ttctgacggc agcgtcagat aacctggcag
     6721 gctggtagag agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc
     6781 gttcacgccg ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt
     6841 acggatgttc tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt
     6901 tgtgcgatcc ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt
     6961 tggcgtacca cagatgtttt caacaacgtc cggcgtgtag cgggaaacgt gcgctttcag
     7021 caggttccac acacagcgcg gatgagtcag tgtttcatcg cgtttcgcat agccgttttc
     7081 atcgaactga tagttccagg acgatttgtc gtactggcgt ttttcagcgt cataaccgct
     7141 gaacaggcca tcttcgaaag caaaatcatc acgcaccagt aggctggcgt tggtgtaatg
     7201 tttaacgtat tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag
     7261 gaacgtaatg tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg
     7321 cgtaaaacgg ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc
     7381 ccagcggaaa ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt
     7441 agcgtttttg atatccaccc agtggttggt catagcacct cgaccaaatg ttggagcaag
     7501 acttgctacc gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag
     7561 ggagcgggca aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag
     7621 cataccggta gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt
     7681 agcgtcacgg tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat
     7741 gcgctgccat ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcact
     7801 gttgacgtaa tccagcaaac cggccccttt cgggcacagc gcaccacggc ttaccggatg
     7861 atccgggtca ccttcaatgt gataaatcgc ttctctggcg ttttttgcgc catcacccag
     7921 gctatacatc aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt
     7981 agcgcgtaat aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa
     8041 tgccgctacc gttgttccag ccatacctcc cgcgcagatt ttaaaaaatt gtctgcgact
     8101 gacgtccatt gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa
     8161 attctgaatc tttttgcgcg aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc
     8221 tgcgcaaatt gacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt
     8281 taaaaaaatg ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc
     8341 atgacacgac aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg
     8401 gtaggattga ttcgcggtgt cagcgagggg ctcggcccgg tcggcggcgc agctgctatc
     8461 tattcattaa gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt
     8521 ccgaaaggct atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg
     8581 ctttccttag ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat
     8641 ctgtggccca gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg
     8701 ctgattgtac caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag
     8761 aatgggttac attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc
     8821 ctggcgttta ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca
     8881 cgcggattta acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac
     8941 tattttctca cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc
     9001 tctgcggcat ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc
     9061 aatgtcacca taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca
     9121 gccgtgctgc tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc
     9181 ggcggttccc tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac
     9241 ggcccggcag ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt
     9301 aattcacctt atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc
     9361 tatttttctc attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat
     9421 gacgccgttt aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa
     9481 atgaaattaa aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac
     9541 gctgccgaag tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct
     9601 ctacgttatt ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc
     9661 tttaaaggag aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat
     9721 tttaaaggct ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat
     9781 gctggtttaa aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc
     9841 tacgacattg gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag
     9901 aaagatgtct tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc
     9961 tttggcttaa ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact
    10021 gacaacagtc atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc
    10081 tcctcaactt atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg
    10141 acaaatgcgc aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa
    10201 ctatgggcca caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct
    10261 gaaacattaa acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt
    10321 gaagtggtgg cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa
    10381 tcgaaaggca gagatctggg ccgctacgcc gaccaggaca tgattgagta tatcgacgtt
    10441 ggtgcgacgt atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg
    10501 attgatgaaa gcgactttac ccgtgccgta gatattcgca ccgataacat cgtcgctacg
    10561 ggcattacct atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg
    10621 atttaagtgc tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc
    10681 attcacctca attaatatta ttccatatcc tccggggaca tcttactata atatttattg
    10741 atga
//
LOCUS       DAJTYI010000126        10677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYI010000126 DAJTYI010000000
VERSION     DAJTYI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..959
                     /locus_tag="OO564_003226"
                     /pseudo
     CDS             1..959
                     /locus_tag="OO564_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1021..1635
                     /locus_tag="OO564_003227"
                     /pseudo
     CDS             1021..1635
                     /locus_tag="OO564_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1692..3149)
                     /gene="pepD"
                     /locus_tag="OO564_003228"
     CDS             complement(1692..3149)
                     /gene="pepD"
                     /locus_tag="OO564_003228"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6654253.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3411..3869
                     /gene="gpt"
                     /locus_tag="OO564_003229"
     CDS             3411..3869
                     /gene="gpt"
                     /locus_tag="OO564_003229"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6654254.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3961..5205
                     /gene="frsA"
                     /locus_tag="OO564_003230"
     CDS             3961..5205
                     /gene="frsA"
                     /locus_tag="OO564_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6654255.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5263..5664
                     /gene="crl"
                     /locus_tag="OO564_003231"
     CDS             5263..5664
                     /gene="crl"
                     /locus_tag="OO564_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6654256.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5703..6758)
                     /gene="phoE"
                     /locus_tag="OO564_003232"
     CDS             complement(5703..6758)
                     /gene="phoE"
                     /locus_tag="OO564_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6654257.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7046..8149
                     /gene="proB"
                     /locus_tag="OO564_003233"
     CDS             7046..8149
                     /gene="proB"
                     /locus_tag="OO564_003233"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6654258.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8161..9414
                     /gene="proA"
                     /locus_tag="OO564_003234"
     CDS             8161..9414
                     /gene="proA"
                     /locus_tag="OO564_003234"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6654259.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9529..9604
                     /locus_tag="OO564_003235"
     tRNA            9529..9604
                     /locus_tag="OO564_003235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9562..9564,aa:Thr,seq:cgt)
     gene            complement(9619..>10677)
                     /locus_tag="OO564_003236"
     CDS             complement(9619..>10677)
                     /locus_tag="OO564_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6654260.1"
                     /translation="RWPVFVDHFKAWRVKNLGESPKKLFKEACIRWLREKSDKKSIDD
                     DKSIISFWMLHFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEY
                     KPKLASKGTKTRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLF
                     AEIAPHFFPVVMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNE
                     TACQILRKQQGLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNF
                     RFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIIS
                     RNGTNTAQEENVVYLNAR"
BASE COUNT         2537 a         2676 c         2794 g         2670 t
ORIGIN      
        1 attacacacc acggcaaatt tacccaacat gcagcgcgta gtggggatgc cagatttaca
       61 ccccggacgc ggctacccga ttggcgcagc gttcttctcc gcaggccgtt tttacccagc
      121 actggtcggc aatgatatcg gctgtggcat ggcgctatgg caaacagata ttctcgctcg
      181 caaatacaac gccgataagt ttgaaaagcg attatcagct ttggatgacg ttgctgaaga
      241 aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggc gcagctcgct
      301 tggctctatc ggtggtggca atcatttcgc tgaactgcaa caagttgatc aaatcatcaa
      361 cgctgaactg tttgcactgg caggtctgga tgcgcagcat ctgcaactgc tggttcatag
      421 cggctcgagg gagctagggc agtctatttt acagcggcat attgcctcgt tttcgcatca
      481 tggtttgcct gaaggcagtg acgacgcgct ggcgtttgcg cgttttaatc gccacctgat
      541 cgctttgcgc ataatgcaac aggttaaggc caccggaagt ccggttctgg atgtggcgca
      601 taactttgtt agcgcgtgtc gaatcggtga tcaacaaggg gtcttacatc gtaaaggtgc
      661 cacgcctgat gactgtggtc tggtggttat tcccggttca cgcggtgatt actccaggct
      721 ggttcaggcc cgtcaggagt gaggaaacat tgcattcgct ggcgcatggg gctgggcgta
      781 aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa atacacagcg acgcaactct
      841 cgcgtactga acttggcagt cgggtaattt gtcgcgataa acaactcatc ttcgaagaag
      901 cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg tctgggcagg ccggattaat
      961 tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag ggaaaaaagg
     1021 atgatcttgc tataactctc ctcagcgcag gggccggaag aatgttgtct cgcagtgaaa
     1081 aaggcgctgg acaggctaat taaagaagct gcccgacaag acgtcgcggt aacggtgctg
     1141 gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg cgctgatttc tctggatggc
     1201 gacaacgcat gggcgttaag cgaaagctgg tgtggcacta ttcagtggat ttgcccgagc
     1261 ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg gcattgggcg ttttaccgct
     1321 gatgagcagg agcaatccga tgcgatccgg tatgagacgc tgcgttcgtc ggggccgggc
     1381 ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc atccggtatt
     1441 agcgtgaagg ttcagtcaga gcgcagccag catgctaaca aacggctggc gcgattgctg
     1501 attgcctgga agctggatca gcagcaatag gaaaatagcg cggtgctgaa atcacagcgg
     1561 cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgtttac agggatggct
     1621 tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc
     1681 aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag tgtccagtaa
     1741 tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt gatagttggc
     1801 ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc
     1861 gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt ttcacgtacc
     1921 agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc tttcgcttcg
     1981 gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta gtctttaccg
     2041 ctgtcgatca gtgaacggat caggcagtga atttctacgt tattgtcagt catggtcacc
     2101 acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt gcggatcaca
     2161 ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca
     2221 gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc
     2281 tcgtttttca ggatctcctg ataggtattc accagagatt tcagtgcgtc gactttatca
     2341 gctgtgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg
     2401 ttgaaatcga taaggcgcag gtccagttct tctgcatgac ccgccaggaa gcgcaccagc
     2461 agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc gcctttcaga
     2521 ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc
     2581 aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc ttcttcttcg
     2641 gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg
     2701 tccataccgg cttcttcggt tatggtcagc agcacttcca gcgggccgtg aaccacgttt
     2761 tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg
     2821 gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc tttcgtgaag
     2881 tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg taagacgacc
     2941 ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt acctacctga
     3001 tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc
     3061 tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc
     3121 tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac
     3181 agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat
     3241 atgtattgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     3301 actcatgtga aatactggtt tttagtgcgc cagatatcta taatctcgcg caacctattt
     3361 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     3421 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     3481 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccggggg
     3541 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     3601 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     3661 tcatcgttat tgatgacctg gtggataccg gtggtactgc tgttgcgatt cgtgaaatgt
     3721 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     3781 actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat atgggcgtcg
     3841 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcattg ccgggcgttg
     3901 ttctttttaa cttcaggcag gttacaatag tttccagtaa gtattctgga ggctgcatcc
     3961 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     4021 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggctcttgat
     4081 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggt
     4141 attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga tgctgaacgt
     4201 accgacgatg atttatacga taccgtgatt ggctaccgtg gtggcaactg gatttatgag
     4261 tgggccaccc aggcgatggt gtggcaacaa aaagcctgta cggaagaaga tccgcaactc
     4321 agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta tcctcatctg
     4381 aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta tgaagaggcc
     4441 gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc
     4501 atcaccggct ttttgcatat gccgcaaggc gatggcccgt tcccgacagt attaatgtgt
     4561 ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg
     4621 cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc aaaatggaag
     4681 ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg
     4741 tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg
     4801 cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt
     4861 cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt
     4921 ctggcgagtc gtttggggat gcatgatgct tccgacgaag cgttacgcgt ggagctgaat
     4981 cgctattcat taaaagtgca aggattgctg gggcgtcgct gcccaacgcc aatgttatca
     5041 ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca
     5101 tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa ttttgacaaa
     5161 ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt tgctaaattt
     5221 tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt accgagtgga
     5281 catccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt
     5341 aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg
     5401 gcaccggaag tgcgtgaatt ctggggctgg tggatggagc tggaagcgca ggaatcccgt
     5461 tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa gagtgttccg
     5521 gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag
     5581 ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcggatga ttttcgtgat
     5641 gaaccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaatatt
     5701 cattaaaact gatacgtcat cccaaccgcg acagtatcat cattattaat attcagctta
     5761 ttatcgctat ccagtttgtt gattttataa tcaacaaacg ctgacatatt tttgttgaaa
     5821 taatacgtag caccgacgtc gatataattg accagatctt catcaccgat gccctcaata
     5881 tctttccctt tcgataagac ataacccagc gatggacgca gaccaaagtc aaactggtat
     5941 tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc cgccagttat tggcgtcatt
     6001 ttgcgtgttt cagaatagaa agttgccaga taaatattat tggcatcgta tttcagacct
     6061 gttgcccatg cttctgcacg cttgcctgtg ccacggcttt gcaggttctg ctcgttggtg
     6121 cgatctgagt tggtataggc cccactaatg gcgaaatcgc tgccgccaaa gtcatatgtc
     6181 aatgacgtgc cgaagccatc gccgttttgc tttttaacgt cgcggttttc gtttttccct
     6241 tgatattgca gggttaagtt caggccatcg ataacgccga agaagtcggt gttccgatac
     6301 gtcgccagac cgctggcgcg tttggtcata aagttgtcgg tctgcgccga ggaatcgcca
     6361 ccaaattccg ggaacatatc ggtccaggct tccacgtcat acaacgcgcc caggttacga
     6421 ccataatcga aagaacccaa atctttatat ttcaacccgg caaaagcgag acgcgttttt
     6481 tgctgtgcag tatcactctc cgctttattt ccggcaaact ccgcttccca ccgaccataa
     6541 ccagtcagtt gatcgttaat ttgagtttca cctttaaaac caaaacggat ataactctgg
     6601 tcgccatctt tactggcgtt atcactcata taatgcatgg ctttaacttt gccatagaca
     6661 tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc agatgccaca
     6721 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6781 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6841 ttaaagatat attacagatt aatgatattc taaaaatgtg gtaatttatt aaatctgtaa
     6901 taaaagcgta aacaactgcc gctacgcttg ctgatcccgc gcaacaaaac gccatgcttt
     6961 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
     7021 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
     7081 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg ttgaacttgt
     7141 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
     7201 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
     7261 acaactgctg gcggcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
     7321 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
     7381 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gatcacaata tcgttccggt
     7441 aatcaatgag aacgatgctg tcgctacggc agagattaag gtcggcgata acgataacct
     7501 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
     7561 aggtttgtat accgctgatc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
     7621 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
     7681 cggcatgagt actaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
     7741 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
     7801 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
     7861 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     7921 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     7981 catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggtgtcagtc gttacaacag
     8041 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     8101 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
     8161 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc
     8221 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     8281 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa
     8341 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     8401 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     8461 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     8521 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     8581 atcctgcgtg gtggtaaaga aacctgtcgc actaacgcgg caacggtggc ggtgattcag
     8641 gacgccctga aatcctgtgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     8701 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     8761 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     8821 ggtataggcg tatgccatat ttacgttgat gaaagcgcag agatcgctga agcattaaaa
     8881 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     8941 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     9001 ggcgtgacat tacacgcaga tgcagctgcg ctgacacagt tgcaggcagg ccctgcgaag
     9061 gtggtggcgg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     9121 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     9181 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     9241 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     9301 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     9361 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     9421 ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg cccggttagt
     9481 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     9541 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     9601 accatttaaa tcaataagtt acctcgcatt taagtaaacc acgttctcct cttgtgccgt
     9661 atttgtgcca ttgcgactta taatcgcatc gattttgctt gcgtgctcgg tgagatgccc
     9721 ggctgaaagg tgggcgtatc tttgaaccat ttcgagagtt tcccatcctc ccatctcttt
     9781 aagtgcaaga agagagacac cggactgaac cagccagctt gcccaggtat gcctcagatc
     9841 atggaagcgg aagttgctaa tgcctgcccg ctttaacgct cccttccatg ccttgttgct
     9901 gtcggttctc atcttcctta ccgctgctgt ttttgttccg tcgcttcggt aggcaggttt
     9961 ggtgtggaca aatacccatc tcttatggag cccctgctgt tttcttaata tctggcatgc
    10021 ggtttcgtta agaggaactc cgatcgcatt gccagctttt gtttcatcag ggtgcatcca
    10081 tgccattttc ttatccagat cgacctgtga ccactcaagg tctgtaacgt tggaacggcg
    10141 aaggcctgtc gtgattgcaa acatgaccac agggaagaag tgaggagcaa tttctgcaaa
    10201 caggcgcttc gattcctcct ctgtaagcca tctgatgcgt ccattcttaa cgcgtggtgt
    10261 tgatattttg ggtgccctgt caagccatcc ccattcaaca gccatattga gaatggcgcg
    10321 aagtattgcc agatgcctcg tcttcgttcc tttgcttgca agctttggtt tatactccgg
    10381 caccggattg ccaagtcgca aacacctgtc ccggctcatc tcccagttca ggcgatggcg
    10441 gcggttttcc atcccgtcta ccgcctccat tattttttct gttgttatgt cagagagaat
    10501 ggcttctctg aagtgcaaca tccagaacga tataatgctc ttgtcatcat caatggactt
    10561 cttatccgat ttctcacgca gccaccgtat gcaggcttcc ttgaatagct ttttcggtga
    10621 ttccccgaga ttttttactc tccacgcttt gaagtggtca acaaaaactg gccaccg
//
LOCUS       DAJTYI010000127        10586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYI010000127 DAJTYI010000000
VERSION     DAJTYI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(124..1152)
                     /gene="malI"
                     /locus_tag="OO564_003237"
     CDS             complement(124..1152)
                     /gene="malI"
                     /locus_tag="OO564_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6654261.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1327..2919
                     /gene="malX"
                     /locus_tag="OO564_003238"
     CDS             1327..2919
                     /gene="malX"
                     /locus_tag="OO564_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6654262.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2929..4101
                     /gene="malY"
                     /locus_tag="OO564_003239"
     CDS             2929..4101
                     /gene="malY"
                     /locus_tag="OO564_003239"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6654263.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4204..5205
                     /gene="add"
                     /locus_tag="OO564_003240"
     CDS             4204..5205
                     /gene="add"
                     /locus_tag="OO564_003240"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6654264.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5241..6281)
                     /locus_tag="OO564_003241"
     CDS             complement(5241..6281)
                     /locus_tag="OO564_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6654265.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6524..6649
                     /gene="blr"
                     /locus_tag="OO564_003242"
     CDS             6524..6649
                     /gene="blr"
                     /locus_tag="OO564_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6654266.1"
                     /translation="MNRLIELTGWVVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6922..7137
                     /gene="ydgT"
                     /locus_tag="OO564_003243"
     CDS             6922..7137
                     /gene="ydgT"
                     /locus_tag="OO564_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6654267.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7223..7663
                     /locus_tag="OO564_003244"
     CDS             7223..7663
                     /locus_tag="OO564_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6654268.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7740..8321
                     /gene="rsxA"
                     /locus_tag="OO564_003245"
     CDS             7740..8321
                     /gene="rsxA"
                     /locus_tag="OO564_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6654269.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8321..8899
                     /gene="rsxB"
                     /locus_tag="OO564_003246"
     CDS             8321..8899
                     /gene="rsxB"
                     /locus_tag="OO564_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6654270.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8892..>10586
                     /gene="rsxC"
                     /locus_tag="OO564_003247"
     CDS             8892..>10586
                     /gene="rsxC"
                     /locus_tag="OO564_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6654271.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2439 a         2735 c         2687 g         2725 t
ORIGIN      
        1 agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg
       61 ccacattttt tatcattaac cttgaggaaa ggacactacc aagtatagca gataaagaga
      121 ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat gcgtctcttc
      181 atgggtgatt ttttgcatca tgcgatccgc aagtgtgata ccaagttccc gcgctggcgt
      241 gctggcccag gtaacaggta tatcatcaag tgtggttggt gtcgcatcgg taaatgccgc
      301 cagagaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt gccgccctgc
      361 tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa ccacggcact
      421 gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct tctggctgga
      481 agtgcactcc aacacccaat cgctgtgaaa cggcaggcca aattttagta gagttgcaca
      541 atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca gccaggcgat
      601 ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct gcatgttgtc
      661 cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa tcaccgggat
      721 agctttttct tctgccatcc gccgcaggtc atcgctgctt cctgcagccc cggcaattac
      781 cacaccatcg acaccctgat tcagtaacag tgaaaaccgc tgtgccagct gctcgccgtc
      841 tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt ccgtcaatcc
      901 ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac caatgacgcc
      961 gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca gctcttcaat
     1021 ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac taagcaccag
     1081 cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt
     1141 ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac gttttacctt
     1201 ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgactc actaaaattt
     1261 gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc tacgaggagt
     1321 cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt ccagcagtta
     1381 ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat gctcggcatt
     1441 ggtagttctc ttagcagcca tgatgtcata accctgatcc cggtcctggg caaccccgtg
     1501 ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag tttcctgcct
     1561 gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaataaagg cgtagcggca
     1621 ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg gttgaccaat
     1681 aaaggcattc tgccaaccac agatgccgcc gttctgaaag ccaataacat ccagagcatt
     1741 cttgggatcc agtcgatcga taccgggatc ctcggtgcgg tgatcgccgg tattatcgtc
     1801 tggatgctgc atgagcgttt ccataatatc cgcctgccgg atgcgctggc attcttcggc
     1861 ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg gccttgtcgg cctggtgatt
     1921 ccattagtct ggccgatttt cgccatgggt attagcggct tgggccatat gataaacagc
     1981 gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt accgtttggt
     2041 ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac gcaggaggtc
     2101 tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag ttgcccgacc
     2161 actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc aaggtaaaat gcctgcgttt
     2221 ctcggcggtc tgccaggtgc agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc
     2281 cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg cggcactacc
     2341 gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg
     2401 ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg taataccgac
     2461 ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac caagtggtac
     2521 atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt ccgtttcgct
     2581 atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag ctcaatcgaa
     2641 aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat cctcgaagca
     2701 ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct gcgtttgtct
     2761 gtggaagata tgtcgcttgt taatgtgcaa gcactgaagg acaatcgggc aattggcgta
     2821 gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa
     2881 gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat gttcgatttt
     2941 tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt cgctgaccgt
     3001 ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc cactgccccc
     3061 tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta cagccgctgg
     3121 aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca ttacaccgca
     3181 attgatacgc agacggtggt gtatggccct tctgtcatct atatggtttc agaactgatt
     3241 cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cgcccgccta tgacgcattt
     3301 tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga gaagcaggct
     3361 gacggctggt tttgcgatat gggcaagctg gaagccgttt tggcgaaacc agaatgtaaa
     3421 attatgctcc tgtgtaaccc acagaatcct accgggaaag tgtggacgtg cgatgagctg
     3481 gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg ttatttccga tgaaatccat
     3541 atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc gcggggagac
     3601 tgggcgttgc tgacgtcggg atcgaaaagt ttcaatattc ccgcgctgac cggtgcttac
     3661 gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa aggacgtgat
     3721 gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta tcagcaaggt
     3781 gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta tatctcagat
     3841 aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac ttatctggcc
     3901 tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag
     3961 caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg tggttttgtc
     4021 cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg attaataaac
     4081 gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaatcgttgc gcaatcgtgg
     4141 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4201 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4261 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4321 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4381 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     4441 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     4501 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     4561 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     4621 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     4681 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     4741 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     4801 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     4861 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     4921 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     4981 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5041 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5101 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5161 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5221 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5281 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5341 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     5401 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     5461 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     5521 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     5581 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     5641 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     5701 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     5761 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     5821 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     5881 tttagcgtca agaaatcgct atcccaacga cggttatgga atacagacag cacacgcccc
     5941 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6001 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6061 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6121 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6181 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6241 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6301 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6361 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     6421 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     6481 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     6541 attaacaggt tgggtcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     6601 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     6661 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     6721 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     6781 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     6841 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     6901 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     6961 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7021 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7081 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7141 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7201 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7261 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7321 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7381 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     7441 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     7501 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     7561 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     7621 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     7681 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     7741 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     7801 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     7861 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     7921 cgtggatttt gatcccactt aatctgattt acctacgcac cctggcattt attctggtga
     7981 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8041 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8101 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8161 ctgtcggttt ctcgctggtg atggtgctct tcgccaccat ccgcgaacgc cttgctgtgg
     8221 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8281 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8341 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     8401 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     8461 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     8521 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     8581 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     8641 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     8701 tccggtagac gccatcgttg gcgctacccg tgccatgcat acggtaatga gtgatctctg
     8761 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     8821 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     8881 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     8941 acggcggcat ccatccaccg gagatgaaaa cccagtccaa cggtaccccc ctgcgccagg
     9001 taccactggc gcagcgtttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9061 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9121 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9181 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9241 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9301 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9361 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
     9421 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
     9481 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
     9541 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
     9601 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
     9661 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
     9721 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
     9781 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctgggaacgc
     9841 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
     9901 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
     9961 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10021 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10081 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10141 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10201 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10261 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcggct cgccttgaac
    10321 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10381 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    10441 aacgcccgga taacagtgca attattgcag cacgggaagc ccgtaaagcg caagccagag
    10501 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    10561 ctgccgttga agcagctatc gcccgc
//
LOCUS       DAJTYI010000128        10379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYI010000128 DAJTYI010000000
VERSION     DAJTYI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..1280
                     /locus_tag="OO564_003248"
                     /pseudo
     CDS             1..1280
                     /locus_tag="OO564_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
     gene            1332..2487
                     /locus_tag="OO564_003249"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="OO564_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="OO564_003250"
     CDS             complement(2453..2575)
                     /locus_tag="OO564_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654272.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..4463
                     /locus_tag="OO564_003251"
                     /pseudo
     CDS             2778..4463
                     /locus_tag="OO564_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4501..6873
                     /locus_tag="OO564_003252"
     CDS             4501..6873
                     /locus_tag="OO564_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6654273.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6930..9713
                     /locus_tag="OO564_003253"
     CDS             6930..9713
                     /locus_tag="OO564_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6654274.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10075..>10379
                     /locus_tag="OO564_003254"
     CDS             10075..>10379
                     /locus_tag="OO564_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6654275.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3068 a         2289 c         2241 g         2781 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgtca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
    10261 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
    10321 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaa
//
LOCUS       DAJTYI010000129        10282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYI010000129 DAJTYI010000000
VERSION     DAJTYI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..872)
                     /gene="rimK"
                     /locus_tag="OO564_003255"
     CDS             complement(<1..872)
                     /gene="rimK"
                     /locus_tag="OO564_003255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6654276.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHAT"
     gene            complement(933..1654)
                     /gene="nfsA"
                     /locus_tag="OO564_003256"
                     /pseudo
     CDS             complement(933..1654)
                     /gene="nfsA"
                     /locus_tag="OO564_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            complement(1638..1925)
                     /locus_tag="OO564_003257"
     CDS             complement(1638..1925)
                     /locus_tag="OO564_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6654277.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            2085..2342
                     /gene="grxA"
                     /locus_tag="OO564_003258"
     CDS             2085..2342
                     /gene="grxA"
                     /locus_tag="OO564_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6654278.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(2372..2749)
                     /locus_tag="OO564_003259"
     CDS             complement(2372..2749)
                     /locus_tag="OO564_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6654279.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            3019..4704
                     /locus_tag="OO564_003260"
     CDS             3019..4704
                     /locus_tag="OO564_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6654280.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            complement(4879..5415)
                     /gene="rcdA"
                     /locus_tag="OO564_003261"
     CDS             complement(4879..5415)
                     /gene="rcdA"
                     /locus_tag="OO564_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6654281.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            5499..6707
                     /locus_tag="OO564_003262"
     CDS             5499..6707
                     /locus_tag="OO564_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654282.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            6707..7522
                     /gene="ybjI"
                     /locus_tag="OO564_003263"
     CDS             6707..7522
                     /gene="ybjI"
                     /locus_tag="OO564_003263"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6654283.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            7608..7893
                     /locus_tag="OO564_003264"
                     /pseudo
     CDS             7608..7893
                     /locus_tag="OO564_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7934..9160)
                     /gene="mdfA"
                     /locus_tag="OO564_003265"
     CDS             complement(7934..9160)
                     /gene="mdfA"
                     /locus_tag="OO564_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6654284.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            9445..10041
                     /gene="ybjG"
                     /locus_tag="OO564_003266"
     CDS             9445..10041
                     /gene="ybjG"
                     /locus_tag="OO564_003266"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6654285.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            10099..>10282
                     /locus_tag="OO564_003267"
     CDS             10099..>10282
                     /locus_tag="OO564_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6654286.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPR"
BASE COUNT         2477 a         2624 c         2564 g         2617 t
ORIGIN      
        1 gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tatcgatacc ggtggttttt
       61 tctattcctt ccagccccgg cgacgcattc acttccatca ccaacggccc gcgattagca
      121 cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt tatcgcgatt
      181 tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccacgatg caaattggaa
      241 cgaaaatagc cctctttcgc ccgccgttca atcgcagcga ccacttcatc accaacaacc
      301 agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg
      361 ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct gacgagtctc cgccagcacg
      421 acgccaattc cctgcgtgcc ttcaaccaac ttgaccacca gcggcgcacc accgaccatg
      481 tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg
      541 ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc aatggcgacc
      601 gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc cgtcccataa
      661 aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa cttgcggcct
      721 ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag cggatcaaga
      781 atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca cgaatagagc
      841 gttccatccc gggacaatat ggcaattttc accctgcacc tctctgtcag acctggtcaa
      901 aagcccgcgt atcatacatc gacgtggcgg ttttagcgcg tcgcccaacc ctgtttgtgc
      961 aaataaccag aataaatggg cggctttctt taatgattgt tcggcggata tgatcgctcc
     1021 aggtatccca gcgattattg ctgccacggg tgaggtaata ttccgccagt tgctcgtcat
     1081 actgcgccag tgcgtcttta tccagcggtt gatagctgtt ttcatgcacc aaaatggagg
     1141 ccggtaaacg cggcttgaga tccggattat ccgcaggcca gccaaggcac agcccaaaca
     1201 gcggcagaac atgctgcggt aatttcagca gtttcgtcac cgcttcaata ttattgcgca
     1261 ggccgccgat atataccccg cccaatccca gcgattccgc tgcggttaat gcattctgcg
     1321 ccatcattgc cgtatcaacg acaccgagca acagttgttc cgccaggccg agctgagcat
     1381 ccggacagat ctgtaaatgg cggttaaagt cggcacagaa cacccagaac tccgccgctt
     1441 gcgctacgtg tttttgcccg ccggtcagcg tcaccagttc ttcacgtaat gctttgtcgg
     1501 taatgcgaat aatgctacta cactgcaaaa aactggaact ggacgtcgca cgggcgctgt
     1561 taataatcgc ctcacactgc gcttcggaaa tgggttcatc agtgaaatgg cgaatggagc
     1621 gacggccaca aataagttca atggttggcg tcattatctt tttctctttc tgaacgtgaa
     1681 tattgcggtg gacggttcat cagctgtggg gcaagacgtt ttaccacctg aagaataacc
     1741 accaccgcag cgggaagcat gagcaaaaca ccgagaaaaa tcatcagaat ctgcacttct
     1801 ggccgagaaa atggctcagg cagcgacagg gagtcgctta ccgacagcag cgccaccgcc
     1861 agtagcatca ttccgataaa ttccagtatc aacacgcctt taggcaattt accgatcgcg
     1921 cgcatacgct tccctctgca aagtgagcct tcagtctaaa acttttcact gtattgtgtt
     1981 taacagttat agcttttagc aattaatgca acaggttaaa cctactttca gcgaatacat
     2041 tttagcgtga tcattacagg cataaatcta tgaggagaga aataatgcaa accgttattt
     2101 ttggtcgttc gggttgccct tactgtgtgc gtgcaaaaga tctggctgag aaattgagca
     2161 atgaacgcga tgattttcag tatcagtatg tagatattcg tgcggaaggg atcactaaag
     2221 aagatctaca acaaaaggca ggtaaacccg tagaaaccgt gccgcagatt tttgtcgatc
     2281 agcaacatat cggcggctat accgattttg ctgcatgggt gaaagaaaat ctggacgcct
     2341 gatcgtctga caagccctcg cgttgagggc tttactgatt ttttctgtgc tgtggtttaa
     2401 acaaactact gataaataag aaacacagtg cccccagcgc acaccagaac accgcgctta
     2461 gtaaccatgc cagctcttgc cagaatgagc gcgtcggtga aaaaaacagc cgcataatga
     2521 gcattgaaca gggtgccgcc agcattgcgc caaatagagg tttcaggact tctctacgct
     2581 gtgaaaaaaa actggcaact gctccaggaa gaatgaaaaa cagcaagccg atttcaggat
     2641 gcccggcagc ccgaaaagcg cctttcatgt gcgtcgccag aaaaaggcac accacaatga
     2701 agaggacaaa acagcagatt gcccccgccc aacgttgttt atgtttcact cgttcctcct
     2761 gacactgcgt ctatcgaaca catttttcgc cagtgtggcg ttcagtaaga taaagccgct
     2821 tcgcattcca tgctaatata ggccaacgca attcatatag ccgttgatac ctaatgtgat
     2881 tacactagta aaatatattg ttactttact atcgtttagg tgcgctgaat gaatctgcac
     2941 cctgaattct ggtaaaacac attatcgtaa attaccattt ctttcaacag cttactagta
     3001 aacaagaagt tagcctccgt gaatataaac gtcgccgaat tgttaaatgg gaattacatt
     3061 ctgttattat ttgtggtcct cgcgcttggg ctatgtctcg gaaagttacg acttggttcg
     3121 atccaactgg gtaattccat tggcgtttta gtcgtatcgc tgttattagg ccaacaacat
     3181 ttcagcatta acaccgatgc gcttaatctt ggctttatgc tgtttatttt ctgcgtcggg
     3241 gtcgaagccg gaccgaactt tttttccatt ttttttcgcg atgggaaaaa ttacctaatg
     3301 ttagcactgg tgatggttgg cagtgcgctg gtgatcgcct tagggttagg taagctgttt
     3361 ggctgggata ttggcctgac ggccggtatg ttagcaggct ctatgacgtc gacaccggtt
     3421 ctggtcggtg ctggcgatac actgcatcat tccggcatgg aaagcaggca gctctcactg
     3481 gcactggata atctgagcct cgggtatgcc ttaacctatt taatcggtct ggtgagtttg
     3541 attgttggtg cgcgttactt gccgaaattg cagcatcagg acttacagac cagcgcccag
     3601 caaatcgccc gcgaacgtgg cctggacact gatgccaacc gtaaggttta tttaccggtg
     3661 atccgcgcct atcgcgtcgg cccggagctg gtggcctgga ccgacggcaa aaatctgcgt
     3721 gaactgggta tttatcgaca aaccggctgc tacattgaac gtattcgacg taacgggatt
     3781 ctggcaaatc cagacggtga tgccgtgcta caaatgggcg atgaaatagc gttggtaggc
     3841 tatcccgacg cccatgcccg actcgatccc agcttccgta acggtaaaga agttttcgat
     3901 cgtgaccttc tcgacatgcg tatcgtcact gaagaagtgg tcgttaaaaa ccataacgct
     3961 gtaggtaaac gtctcgcaca actgaagttg accgatcacg gttgcttcct taaccgcgtc
     4021 attcgtagcc agattgagat gccgatagat gacaacgtcg tgcttaacaa aggtgacgtt
     4081 ttacaagtca gcggtgatgc ccgtcgcgta aaaaccatcg ccgatcgcat cggctttatc
     4141 tcgattcaca gccaggtcac tgacctgctg gcattttgcg ccttctttgt tattgggctg
     4201 atgatcggga tgatcacctt ccagttcagc acattcagtt tcggcatggg gaacgctgcc
     4261 gggttgttat tcgccggaat tatgctgggc tttatgcgtg ctaaccaccc gaccttcggt
     4321 tacattccgc aaggtgcatt aagcatggtg aaagagttcg gcttgatggt gtttatggca
     4381 ggcgttggtc tgagcgccgg tagcggtatt aataacggcc tgggcgcgat tggcggtcag
     4441 atgttgattg ccggattaat tgtcagtctt gtgcccgtgg ttatctgttt cttgttcggt
     4501 gcttatgtat tgcgaatgaa ccgcgcactg ttgttcggcg caatgatggg cgcacgcacc
     4561 tgcgcgccgg caatggagat catcagtgat acagctcgca gtaacatccc tgcgctgggc
     4621 tatgcgggca cctacgcaat caccaacgtc ctgctgacgc tggcagggac aatcatcgtc
     4681 atggtatggc caggattagg ataaaactga agttgccctg aaaatgaaat ttttttgcac
     4741 aaccgcagaa cttttccgca gggcatcagt cttaattagt gccactgctt ttctttgatg
     4801 tccccatttt gtggagccca tcaaccccgc catttcggtt caaggttgat gggttttttg
     4861 ttatctaaaa tttatctact accctgcaac cctctcaacc atcctcaaaa tctcctcgcg
     4921 cgatagcggc ttacggtcgg tgacaaaatg cagcgtcatc ccctcaataa acgcatcaag
     4981 agcgcgggcg gttccgggtt caaaccattg ttcgagcgtt tgctgactgc gctgcatcca
     5041 gttttgcatt accgttttta atagcggttt tcggctagcc agcgcgtaga gctggtacat
     5101 cagctccata ttatccggcg ttgcaacctg tgagctgtag atcatatcgg tgatagcctg
     5161 gcatgcgcct tgagcatcac taacatcgct aaaaaatgcc tgatattgcc gggacataat
     5221 ctcagtaaaa ctgctgaacg cttccagcaa caactcatca attcctgaaa aatagtaggt
     5281 catcgatccc aacggtaccc cggcaagggt agcaattttg cggtgcgtaa cagcatgtat
     5341 tccgtaaagt ttcaccgcct ccagcgtggc ctggataatt ttttctcgcc gttgcggatc
     5401 gttagcgcga cgcatgtcat ttcctctgtc tgcaattgtg tacaaatgta cacaaccttg
     5461 ctaatgttgt cttccctctt cttattttga cgtgatctat gaccgtaaat tcttcacgta
     5521 atgcattgaa acgccgaacc agggcgctgt ttatgttctt ctttttgcca ggcctgttaa
     5581 tggcgtcctg ggcaacccgt acgcctgcta tccgcgatat tctctctgtc tcgatcgctg
     5641 aaatgggcgg tgttctcttt ggtctgtcga tcggttcgat gagcggtatt ctctgctcgg
     5701 cgtggttagt gaaacgcttt gggacacgta atgtcatcct ggtcacgatg tcctgcgcat
     5761 tgatcgggat gatgatatta agtctggcac tctggctgac atcgcccctg ctctttgccg
     5821 ttggtctcgg cgtctttggg gcaagttttg gttctgcgga agtggcgata aacgttgaag
     5881 gtgccgccgt tgagcgagaa atgaataaaa cggttttgcc gatgatgcac ggtttttata
     5941 gcctgggcac gctggcaggc gctggtgtcg ggatggcact gacggccttt ggcgttccgg
     6001 caacggtgca cattttattg gcggcgctgg taggtatcgc gcctatttat atcgccattc
     6061 aggcaatccc tgacggtacg ggcaaaaatg ctgccgatgg cacccagcat ggcgaaaaag
     6121 gcattccttt ttatcgcgat atccagttgc tgttgattgg tgttgtagtg ctggcgatgg
     6181 cctttgccga aggttctgcc aacgactggt tacccttatt aatggttgat ggtcacggtt
     6241 ttagccccac ttccggctca ctgatttatg ccggttttac cctggggatg accgttggac
     6301 gcttcaccgg cggttggttc atcgaccgtt acagtcgcgt tgccgtggtt cgggccagtg
     6361 cactaatggg ggcgttgggt attgggctga ttatttttgt cgatagcgcc tgggtcgcag
     6421 gagtgtctgt tgtactttgg ggactgggtg cctcgctggg cttcccgctg accatttctg
     6481 ccgccagcga taccggcccc gatgcaccaa cccgcgtcag tgtggtagca acgaccggtt
     6541 atctggcttt cctcgtcggg ccgccgctgc tgggctatct cggcgaacat tatggattac
     6601 gtagtgcaat gctggttgta ctggcgctgg ttattctcgc ggctattgtc gcgaaagccg
     6661 tcgccaaacc cgataccaaa acgcagacgg cgatggagaa tagttgatga gtattaaatt
     6721 aattgcggta gacatggatg gtactttctt aagcgatcaa aaaacctata accgtgagcg
     6781 gtttatggct cagtatcagc aaatgaaagc acaaggcatt cgctttgtgg tcgccagcgg
     6841 gaatcaatat tatcagttga tctctttctt ccctgaaatt gcgaatgaaa ttgcctttgt
     6901 ggctgaaaac ggcggctggg tagtgagtga aggcaaagat gtttttaatg gcgagctgtc
     6961 gaaggatgcg tttgctactg tcgtggaaca tttgctgacg cgcccggaag tggaaattat
     7021 tgcctgcgga aaaaatagcg cctatacact caaaaaatat gacgatgcca tgaaaacggt
     7081 ggcggaaatg tattatcacc gtctggaata cgtcgataac tttgacaact tagaagatat
     7141 tttctttaag tttggcctga atctttccga tgaactgatt ccacaagtac aaaaagcatt
     7201 acatgaggcc atcggcgata ttatggtgcc agtccacacc ggcaacggca gcatcgatct
     7261 gattatcccc ggcgtacata aagccaatgg ccttcgccaa ctgcagaaat tatggggaat
     7321 agacgacagc gaagtggtgg tctttggcga tggcggtaac gatattgaga tgctgcgtca
     7381 ggcaggcttt agttttgcaa tgaaaaatgc cggcagcgcg gtcgtcgcag cggcaaaata
     7441 ccgggcaggc tccaataacc gtgaaggcgt actggatgtg atcgataaag ttcttaaaca
     7501 cgaagcgcca tttgaccaat aaaaacggca gcacgcccgg caacacaatg cctgaatgaa
     7561 ggctgcataa ttcgcggaaa ttaacccgct gcattatttc aggcgaaatg attatgaaaa
     7621 attgtctact gttgggcgcg cttttaatgg gctttactgg tgtggcgatg gcgcaaagtg
     7681 tcaccgtcga tgtgccgagt ggatacaaag tggttgtggt tcctgattca gtcagcgttc
     7741 cacaagcgtg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc tgtgccagct
     7801 ccggcttacc gcccgcatcc ttatgttcgc tatcttgcca gcgtaggtga agggatggtt
     7861 atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc gcacaaatca
     7921 atcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt aaaaagataa
     7981 ccatcagcga cagccacaaa attccgttga caaggttgaa gagattaaac agtccgttgc
     8041 ccccgttcag ccaggcatgt ttgctgattt caataccgac ggtaaagatc agcatttgca
     8101 gcattcccat cgccgcagaa accgtacctt tactcatatc gctggcaaac agggttaatc
     8161 gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac ccggcggtca
     8221 tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac agaccaatca
     8281 taatcggcca gccgcccata ataatcagcg aacgtacggt gcggcgcgag gtcagacgcg
     8341 ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc aagccatatt
     8401 catagctgct caactgctcc ccggtaatga tgataatcgg cgactgggcg atccacgcca
     8461 gcaatggcag gctaacgaat cccagcgccc ccgccacaaa gcggccgttc ttcagcacca
     8521 gcttatagtc acgaccgagt tctttcagtg acagtttctc gcctatacgc gtggcggttt
     8581 caggcatagc tcgttgcaga ccgaaaaagg agatcgctgc caatgcggca aacaaaacaa
     8641 acatcccctc ccaaggcagc acatggatcc acgccgcgcc caccagcgga ccaagtagcg
     8701 gagcaatcag cgccacgttc accatcagcg cggtgatctt gatacaaacc gcctcttcga
     8761 aggattcctg aattgcggcg tatcccacag cgccaatgaa acagaggctt atgccctgca
     8821 agaagcgcaa catggtgaat tgttcaatgt tttgcgccag taatattgcc agacaggtga
     8881 cgataaacca caccacaccc gccagcatca ccggacggcg accaatacga tccgacagcg
     8941 gccccaggag ccattgtaaa aacatcccgc ccgccagata cgcggtcatc gaagtaggaa
     9001 cccaatcaat gcccgcctga tattgttcca ccacggccaa cataccgggt tggatcatat
     9061 cgttgccgat ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct
     9121 gacgtccaag cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat
     9181 aatcgcgcag agtttaataa aagcgcagct aacgagaaag cgaattttgt agctgaaacc
     9241 acggttaagc acattcttac attattgcga gtatagctac gctttcttta agttttattt
     9301 aacctatgcc cgttacaatc acccaccgta aacaggccgc ttgagggaaa taagacgatg
     9361 ccgctttacc cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt
     9421 ttaatcactt tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt
     9481 attaacgcga cgccagactc ggctccgtgg atgatctcgt tggcgatttt tattgctaaa
     9541 gatttgatta ccgtggtgcc gttgctggcc gcggtacttt ggttgtgggg gcttacagca
     9601 caacggcaac tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg
     9661 acgatgggac atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc
     9721 ctgcatcatg cggcggatga ctcattcccg agcgatcacg gtacggtgat tttcaccttt
     9781 gcactggcat ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc
     9841 gtcgtcattg cctggtcgcg cgtttatctt ggcgtccact ggccgctgga tatgctcggt
     9901 ggattgctgg caggtatgat tggctgcctt agtgcccaga ttatctggca agcgatgggg
     9961 cataaactct atcaacgtct gcaatcgtgg tatcgcgtct gttttgcatt accgatccgc
    10021 aaaggctggg tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa
    10081 taactattca gagggattat ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa
    10141 gaattaaaac gcagcgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg
    10201 gagatgacga ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc
    10261 tatattgttc tggaaccgcg ca
//
LOCUS       DAJTYI010000130        10240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYI010000130 DAJTYI010000000
VERSION     DAJTYI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO564_003268"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO564_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6654287.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO564_003269"
     CDS             complement(1152..1238)
                     /locus_tag="OO564_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6654288.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO564_003270"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO564_003270"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6654289.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO564_003271"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO564_003271"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6654290.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO564_003272"
     CDS             3587..3631
                     /locus_tag="OO564_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654291.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO564_003273"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO564_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6654292.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5390..5476
                     /gene="leuL"
                     /locus_tag="OO564_003274"
     CDS             5390..5476
                     /gene="leuL"
                     /locus_tag="OO564_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6654293.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5569..7140
                     /gene="leuA"
                     /locus_tag="OO564_003275"
     CDS             5569..7140
                     /gene="leuA"
                     /locus_tag="OO564_003275"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6654294.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7140..8231
                     /gene="leuB"
                     /locus_tag="OO564_003276"
     CDS             7140..8231
                     /gene="leuB"
                     /locus_tag="OO564_003276"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6654295.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8234..9634
                     /gene="leuC"
                     /locus_tag="OO564_003277"
     CDS             8234..9634
                     /gene="leuC"
                     /locus_tag="OO564_003277"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6654296.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9645..>10240
                     /gene="leuD"
                     /locus_tag="OO564_003278"
     CDS             9645..>10240
                     /gene="leuD"
                     /locus_tag="OO564_003278"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6654297.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPA"
BASE COUNT         2669 a         2688 c         2517 g         2366 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggcttagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccat ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccagc cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaagcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaatgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cacccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccatttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgtttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat
     4981 catctacaaa atggattaaa tgtgcttatt taataattat tttatatatt taatgcatta
     5041 attcttaaca ttaattgatc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa
     5101 catataattc aatacaaatc attaggatag gttttgcaac gcgtgcattt tgtccccttt
     5161 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
     5221 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
     5281 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
     5341 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
     5401 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
     5461 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
     5521 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
     5581 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
     5641 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
     5701 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
     5761 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
     5821 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
     5881 atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg cgctatctat
     5941 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
     6001 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
     6061 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     6121 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     6181 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     6241 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     6301 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccag
     6361 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     6421 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     6481 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     6541 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     6601 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     6661 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     6721 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     6781 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     6841 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     6901 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     6961 gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     7021 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     7081 gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga aaccgtgtga
     7141 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     7201 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     7261 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     7321 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     7381 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     7441 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     7501 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga
     7561 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     7621 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     7681 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     7741 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     7801 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     7861 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     7921 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     7981 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     8041 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     8101 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     8161 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     8221 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     8281 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     8341 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     8401 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     8461 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     8521 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     8581 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     8641 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     8701 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     8761 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     8821 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     8881 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccggtctgg ttgctcctga
     8941 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     9001 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     9061 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     9121 ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     9181 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     9241 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     9301 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     9361 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     9421 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     9481 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     9541 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     9601 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     9661 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     9721 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     9781 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     9841 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     9901 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     9961 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
    10021 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
    10081 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
    10141 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
    10201 acgacgcgat tgcgtcttat gaagcaaaac aacctgcgtt
//
LOCUS       DAJTYI010000131        10199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYI010000131 DAJTYI010000000
VERSION     DAJTYI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            21..824
                     /gene="hpaI"
                     /locus_tag="OO564_003279"
     CDS             21..824
                     /gene="hpaI"
                     /locus_tag="OO564_003279"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6654298.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            846..2201
                     /gene="hpaX"
                     /locus_tag="OO564_003280"
     CDS             846..2201
                     /gene="hpaX"
                     /locus_tag="OO564_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6654299.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2211..3101
                     /gene="hpaA"
                     /locus_tag="OO564_003281"
     CDS             2211..3101
                     /gene="hpaA"
                     /locus_tag="OO564_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6654300.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3128..4065
                     /locus_tag="OO564_003282"
                     /pseudo
     CDS             3128..4065
                     /locus_tag="OO564_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4292..5850
                     /gene="hpaB"
                     /locus_tag="OO564_003283"
                     /pseudo
     CDS             4292..5850
                     /gene="hpaB"
                     /locus_tag="OO564_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5868..6380
                     /locus_tag="OO564_003284"
     CDS             5868..6380
                     /locus_tag="OO564_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6654301.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6773..8923
                     /gene="btsT"
                     /locus_tag="OO564_003285"
     CDS             6773..8923
                     /gene="btsT"
                     /locus_tag="OO564_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6654302.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8973..9176
                     /locus_tag="OO564_003286"
     CDS             8973..9176
                     /locus_tag="OO564_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6654303.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9187..10143
                     /gene="yjiA"
                     /locus_tag="OO564_003287"
     CDS             9187..10143
                     /gene="yjiA"
                     /locus_tag="OO564_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6654304.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2430 a         2653 c         2637 g         2479 t
ORIGIN      
        1 ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa gcaggccgtc
       61 cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta ctggccggac
      121 caggattcga ctggttattg atcgacggtg aacacgcgcc gaataacgtg caaaccgtgc
      181 tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc ccatcgtgga
      241 acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta ctggtgccga
      301 tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat ccccccgccg
      361 gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc attcctgatt
      421 acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa
      481 tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt atcggcccgg
      541 cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa gtacaggccg
      601 ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg atcctgatcg
      661 ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg
      721 ttgacaccac cctgctcgcc cgcgccgccg aagcgctggc agcacggttt ggtaatagtt
      781 cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac gatgtggagc
      841 gcatcatgac gacctctacc ctgcaatata aagataataa aactgttgaa attgaacatc
      901 gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg tttgtttttt
      961 cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag gatcttggcc
     1021 tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac gtcatgtgcg
     1081 ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc gctgggatca
     1141 tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc gaaacactct
     1201 atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt attttggttt
     1261 atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta tttatgatag
     1321 cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg gccttggatg
     1381 gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg ccatcggtca
     1441 ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg acctggcttg
     1501 atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag agtgcatttg
     1561 cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc atcatgctat
     1621 atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc tggacgccgc
     1681 aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg ctggcggcca
     1741 tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc gaccgtctta
     1801 aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga tggttactcg
     1861 cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc tcaatgggat
     1921 catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc ttgcaatcgc
     1981 gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca gtcagtccat
     2041 tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg tggtttgtag
     2101 caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg agtcgggaac
     2161 actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg atgtccagtg
     2221 aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat gatgcccgct
     2281 acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc tttggacgcg
     2341 atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc actggacgca
     2401 ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt gtactaacac
     2461 caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat gttctgaccg
     2521 tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt ggagatgccg
     2581 caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg gcggcattaa
     2641 atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga cgggaaattc
     2701 tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca ccaccaaatg
     2761 atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt aatcggctga
     2821 ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg ctcggtatga
     2881 gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg aagagattga
     2941 ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag caaagcatca
     3001 accagatagc ccttgatctt ggctacaaag atcccgccta tttcgcccgt tttttcaacc
     3061 gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc aacctgagag
     3121 actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg cgccagtggc
     3181 attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa cgaaaggtgg
     3241 cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta ttggcaataa
     3301 aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa ataatattcc
     3361 actgcgccag attgataata ttttactcac ccatttacac agtgatcata ttctggacta
     3421 tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta ttcatgtatt
     3481 cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg atcgggacat
     3541 cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta cagctcaaga
     3601 tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat taaaggtccc
     3661 tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac aaggcaagcg
     3721 aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct cagaaggcgc
     3781 tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg cagacagtat
     3841 tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta ttgaggatgt
     3901 aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc tggtaccaat
     3961 caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagt ccctatcgtc
     4021 gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat tccgtccccg
     4081 ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat cacgatcgca
     4141 gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg aaccgtctct
     4201 tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca aaattaacaa
     4261 tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc gcgccagtac
     4321 ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc gcgagatcta
     4381 tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta atgcggcagc
     4441 gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact ctctgtgttg
     4501 gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg cgaaaagtgc
     4561 cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga gctatggctg
     4621 gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg cgaatccggg
     4681 cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc aggaaactgg
     4741 cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac cgataaagtg
     4801 aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt cagcggtgcg
     4861 aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca
     4921 caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat
     4981 ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac cggctcgcca
     5041 tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt gatggataac
     5101 gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg ccgtcgctgg
     5161 acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg
     5221 aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg caccctggag
     5281 ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca
     5341 ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta tttaccggat
     5401 catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac
     5461 attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc ccgtgacctg
     5521 aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa cggtatggat
     5581 cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag cgaatttggt
     5641 ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat tcgcctgcaa
     5701 tgtctgcgct aggcacaaag ctccggcaat atggacaaga tgatggcgat ggttgatcgc
     5761 tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa caacgacgat
     5821 atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg caattagatg
     5881 aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta aatattatca
     5941 ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc tcggtcacgg
     6001 atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac ccggtttttc
     6061 agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg atggcgcgcc
     6121 acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca tgctggcaaa
     6181 aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa ggagagatcc
     6241 gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa aacatcatcc
     6301 tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg gtgatgctgg
     6361 aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg ggcattttta
     6421 ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc tcaccgccat
     6481 tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt tacgcgcacg
     6541 tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa
     6601 cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt ttgcaggaag
     6661 gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt gaacgaacgc
     6721 agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta ctatggatac
     6781 gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg cattctgcct
     6841 cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc
     6901 tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc agaaggtgat
     6961 gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc tgaactacgt
     7021 tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc
     7081 gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc tgtggctgct
     7141 ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta tctcctctcg
     7201 ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag taccggggac
     7261 tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc tggcgctgat
     7321 tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct caaccgtacc
     7381 gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt
     7441 ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg tgattgctca
     7501 cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct tcgcgctgat
     7561 tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta
     7621 tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct
     7681 gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg gcccgctgtg
     7741 gaaaggcgct ctattcccgt tcctgttcat caccatcgcc tgtggtgcgg tatctggctt
     7801 ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg
     7861 tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg cgctggttgc
     7921 agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg ctggccttgg
     7981 catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca tcatggcgca
     8041 gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct tcgtgatttc
     8101 gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc
     8161 aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag tgctgccgat
     8221 ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt tcatcctgac
     8281 cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc tgggtaactt
     8341 catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta ctgcgggctg
     8401 tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa
     8461 gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag cgctggtact
     8521 gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa ctgttgttcc
     8581 ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa
     8641 cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga ttgctaacgg
     8701 tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga acaactacac
     8761 caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct tctacggttt
     8821 caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa caccgtacgt
     8881 tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt tagccccgct
     8941 tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga caggcaaaaa
     9001 aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac aactacgtcg
     9061 agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg
     9121 aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga
     9181 gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc gggcaaaact
     9241 accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt gattgaaaac
     9301 gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac gcagatcaaa
     9361 acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga cgcactactc
     9421 gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt gattgaatgc
     9481 accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga gattttatgc
     9541 cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc cgatgagcag
     9601 atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat tctgctgact
     9661 aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg catcaacgcc
     9721 cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct gttcaacacc
     9781 aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg
     9841 gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt agatatcagc
     9901 gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact gctgcgttac
     9961 aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc
    10021 ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag cacaatggtg
    10081 tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg attgaggaag
    10141 taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac gagtggaac
//
LOCUS       DAJTYI010000132        10150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYI010000132 DAJTYI010000000
VERSION     DAJTYI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(92..421)
                     /gene="mdtI"
                     /locus_tag="OO564_003288"
     CDS             complement(92..421)
                     /gene="mdtI"
                     /locus_tag="OO564_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6654305.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(408..773)
                     /gene="mdtJ"
                     /locus_tag="OO564_003289"
     CDS             complement(408..773)
                     /gene="mdtJ"
                     /locus_tag="OO564_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6654306.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1184..2218
                     /gene="tqsA"
                     /locus_tag="OO564_003290"
     CDS             1184..2218
                     /gene="tqsA"
                     /locus_tag="OO564_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6654307.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(2243..3631)
                     /gene="pntB"
                     /locus_tag="OO564_003291"
     CDS             complement(2243..3631)
                     /gene="pntB"
                     /locus_tag="OO564_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6654308.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3642..5174)
                     /gene="pntA"
                     /locus_tag="OO564_003292"
     CDS             complement(3642..5174)
                     /gene="pntA"
                     /locus_tag="OO564_003292"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6654309.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5698..6642
                     /locus_tag="OO564_003293"
     CDS             5698..6642
                     /locus_tag="OO564_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6654310.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            6828..8210
                     /locus_tag="OO564_003294"
     CDS             6828..8210
                     /locus_tag="OO564_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6654311.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8247..8969
                     /gene="folM"
                     /locus_tag="OO564_003295"
     CDS             8247..8969
                     /gene="folM"
                     /locus_tag="OO564_003295"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6654312.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(8966..9301)
                     /locus_tag="OO564_003296"
     CDS             complement(8966..9301)
                     /locus_tag="OO564_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6654313.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9430..>10150
                     /gene="rstA"
                     /locus_tag="OO564_003297"
     CDS             9430..>10150
                     /gene="rstA"
                     /locus_tag="OO564_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6654314.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDS"
BASE COUNT         2555 a         2626 c         2434 g         2535 t
ORIGIN      
        1 ccatttctgt taccggtttt cgcgacaagc tggatcaact gtcgcaaaaa taatgttcaa
       61 acgctgcccg acagcgcggg cagcgtcttc atcaggcaag tttcaccatg atcattccag
      121 ccaacagcaa gaccaggcca atccagcctt tacgatttaa ccgttgacca aacaagatcc
      181 aacctgcggc taacgtggcg gcaataccaa acccgcccca caatgcataa gcgacagaca
      241 agtcgatccc tttaacggcc tgagaaagcg cactaaaggc cgccagcacc gccgccaggg
      301 agagcaagcc aaatattttg cgacgaaagc cgtcagaaaa tttcaaaaag acgttagcaa
      361 cgatttccag cacgattgcc aatgccagcc aggcggcgtg aacccattca aactgcgcca
      421 tggttcacct ccagttcagg tttacgcgct ttacgggtac ctgatttgat caacactatc
      481 ccggcgacca gggtggttaa cccggcaatt ttcatcagcg ataaactttc gtcgaataac
      541 aaaacgctaa acaaggtaat aaataaaata ccgatacctt cccacagcgc ataagctacg
      601 cctaaggcga tttttttaac ggcgaaagag agaaatatat acgacagaga aatcatcacc
      661 agcattaaaa taaagccgcc atttccctca ctgacgctcg cccatttcat tgacagcgta
      721 ccggtaattt ctgtagcaat agccagacct aataaaatcc aataaatata cattgtcctt
      781 ctcctgcaag agaactattt taattttcgc ttaattcagc gaaaccaaag taaagtggtc
      841 aagccctgcg catagggcaa gcttaggcag aagaaaggac taaagcgcgt tgcgccagtg
      901 ctgctcacct acgagcaaga tagtggatga ggtacgaaga tgggtaaatg tagaaaataa
      961 cgtcctgaac aaattgtcca taatattaca attatccgca gtgttgcttc tcgtcatcgc
     1021 agatgataat tgtcctcggt agttgaacac gcctgatttg tatcatagct taagaattaa
     1081 ctcaaaatat tttcacttct ttacctgagc ggtttgattt tcgttatgat gacggagcga
     1141 aaaagacatt attattagca aaggaagaaa aacggggaca agcatggcaa agccgatcat
     1201 cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg
     1261 tatccgtttt gccgccgaga tcatcgtgcc gtttattctc gcattattta ttgctgttat
     1321 tcttaacccg ctggtgcaac atatggtccg ctggcgtgtg ccgcgtgtac tggcagtgtc
     1381 gattttgatg acaatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc
     1441 gctcaacgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     1501 agctcttgag ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc aactggcgca
     1561 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     1621 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc
     1681 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     1741 tcaacgtgcg attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat
     1801 caccggcttg gtcgcctggg cgatgctcgc cgcactcgat gttcacttcg cttttgtctg
     1861 gggattgctg gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat
     1921 cccccctatc gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     1981 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     2041 tgggctgggg ctttcgacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     2101 aggaccggtg ggtatgctgc tttccgtgcc gttgacaatt attgtcaaaa ttgcgcttga
     2161 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     2221 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     2281 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     2341 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     2401 caggcatacc agcaatcgga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     2461 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     2521 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     2581 aacgccccgc aaccgggtgg ataccgaaac gcacattaat actacgagcg cggagtttct
     2641 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     2701 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     2761 gctcacccac ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     2821 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcccccg
     2881 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag
     2941 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccaccggc atatctgcac
     3001 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     3061 atgccagcac ttgcaggccg acgctgtccg tgcgaacaaa tacaatcagc agcaagaagg
     3121 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     3181 aaatcttgcc acacagtttg ccgaacgcca ccaccgagcc cgtgaacgtt accgccccga
     3241 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     3301 cgtcatgatg cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctgt
     3361 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agccggatac
     3421 caattgcccc accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc
     3481 caaaaatggt tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc
     3541 gagacgtttc atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa
     3601 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     3661 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     3721 gcgataaaac taaggaagct aacccagcct ccctggccaa tctgcaacag tgctccgaca
     3781 acaataatcc ctgaaatcgc gttggtaacc gacatcaacg gtgtatgcag cgcgtgcgat
     3841 acattccaca ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca
     3901 agaaactctt tcggcgcaac gcttgacagc caaccgaaca ggatgattgc cagcgccatc
     3961 aacgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     4021 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     4081 tcgcccgcac ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata
     4141 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc
     4201 tgtgaggatt gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca
     4261 ttttccgtac tgaagatttc acccggcacg atgtattcac agttgccacc gttttgggct
     4321 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     4381 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
     4441 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
     4501 ccatcgccgc tgcccgcttc ctcgttaaaa tccagctcga ggaattccgc gcccatactt
     4561 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
     4621 tttgctgcgc caatggcggc caaacctgca acaccggcac caatcaccat cacttttgcc
     4681 ggtggcactt tcccggccgc agtgatttgc ccggtaaaga agcgcccaaa ttcatgtgcc
     4741 gcttcaacaa tggcgcgata accggcgata ttcgccatcg agcttagtgc gtccagcgat
     4801 tgtgcgcgtg agattcgcgg cacagagtcc atcgccatca cggtcacgtt acgttccgca
     4861 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtcccg
     4921 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
     4981 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
     5041 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
     5101 tgttccactg tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
     5161 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
     5221 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
     5281 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
     5341 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
     5401 cgccggataa agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc
     5461 acgaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat
     5521 gctgatacgg ctgtttttta agcatagacg gtcatttgag caggattaaa attggtttaa
     5581 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
     5641 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
     5701 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
     5761 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
     5821 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
     5881 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
     5941 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
     6001 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
     6061 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
     6121 aaagcggcaa aagcgaaagg tgcctactct ttctacatcg ttcgtcaaat cgatgccaac
     6181 cagggcggca accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
     6241 gtccagagcc cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
     6301 ggtggcgaag ccgcgaagaa agttgagatc ccgggtgttg cgactaccgc atcaccaagt
     6361 tctgaagtcg gtcgcttctt tgaaacccag tcatcaaaag gcgggcgtta caccgtcacg
     6421 ctcccggatg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
     6481 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
     6541 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcagaaa
     6601 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
     6661 aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat
     6721 gcagacacat cttttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
     6781 tgccctaata tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac
     6841 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
     6901 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
     6961 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc
     7021 ccgaacttga tggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
     7081 tttgttccgc atggggatac tggctgtgcg cggtcatcgc caacgtctcc tatctggtga
     7141 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg tttggcgatg
     7201 gtaatacctg gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga
     7261 tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt
     7321 tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc
     7381 tcgacttcac cggacttgca cttggcgtac ctgtttggga acaggtgaaa aacaccatgc
     7441 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
     7501 gtaataaacg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
     7561 acttgctggt aacgctgctt tcgctgggtg tggtggcacg tcctgaactg gctgaaattc
     7621 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca
     7681 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg
     7741 cagaagttcc gttcctggct gccactcata aagcattccc acgcattttt gcgcgccagg
     7801 acgcacaagc tgcgccatcc gcctcactgt ggttgaccaa catctgcgtg caaatttgtc
     7861 tggtactcat ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa
     7921 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
     7981 tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt atggttattg tacgcctctg
     8041 gtccaatgca cttgctgtta tccgttgttc tgtatgcacc ggggctgctg gttttcctgt
     8101 atgcgcgcaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
     8161 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc
     8221 atcgttggat agcaaaggag ataacgatgg gtaaaaccca gcccttgcca atattaatta
     8281 ctggcggagg tcgtcgcatc ggcctcgccc tcgcatggca tttcattaat caaaagcaac
     8341 cggtgattgt cagctatcgg acacactatc cagccattga tggactgatt aatgcaggtg
     8401 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
     8461 tactaaaaac cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggattgcgg
     8521 aaaaaccggg tgcgccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata
     8581 ccccatacct gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca
     8641 gcgatatcat tcactttacc gattatgtgg tggagcgcgg cagcgacaaa catgttgcgt
     8701 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
     8761 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
     8821 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
     8881 aagtgatcga cctggtcgat tacttactta ccagttgctt tgtcaccgga cgcagtttcc
     8941 cacttgatgg cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc
     9001 atgcgcttat cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc
     9061 cggtgaaata ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa
     9121 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
     9181 taagcgcaaa gatcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
     9241 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
     9301 tctttttacc ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt
     9361 tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc
     9421 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt
     9481 gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc
     9541 gaagaaacca ttttgcgaga aaatccggat ttggtgttac tcgacatcat gctaccaggc
     9601 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt
     9661 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
     9721 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
     9781 cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa
     9841 gccctgcatt tcggcacgtt aactatcgac cccatcaacc gcgtagtcac cctggctaac
     9901 actgaaatct cgctctcgac agctgatttc gaattattgt gggaactagc tacgcatgcc
     9961 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    10021 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    10081 gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca
    10141 tgggatagtg
//
LOCUS       DAJTYI010000133        10065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYI010000133 DAJTYI010000000
VERSION     DAJTYI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            290..1006
                     /locus_tag="OO564_003298"
     CDS             290..1006
                     /locus_tag="OO564_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6654315.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1462..1537)
                     /locus_tag="OO564_003299"
     tRNA            complement(1462..1537)
                     /locus_tag="OO564_003299"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1502..1504),aa:Asn,seq:gtt)
     gene            complement(1638..3265)
                     /gene="yeeO"
                     /locus_tag="OO564_003300"
                     /pseudo
     CDS             complement(1638..3265)
                     /gene="yeeO"
                     /locus_tag="OO564_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3270..3345
                     /locus_tag="OO564_003301"
     tRNA            3270..3345
                     /locus_tag="OO564_003301"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3303..3305,aa:Asn,seq:gtt)
     gene            complement(3383..4333)
                     /gene="cbl"
                     /locus_tag="OO564_003302"
     CDS             complement(3383..4333)
                     /gene="cbl"
                     /locus_tag="OO564_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6654316.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4435..5352)
                     /gene="nac"
                     /locus_tag="OO564_003303"
     CDS             complement(4435..5352)
                     /gene="nac"
                     /locus_tag="OO564_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6654317.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5679..5754
                     /locus_tag="OO564_003304"
     tRNA            5679..5754
                     /locus_tag="OO564_003304"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5712..5714,aa:Asn,seq:gtt)
     gene            complement(5810..6745)
                     /gene="ldtA"
                     /locus_tag="OO564_003305"
     CDS             complement(5810..6745)
                     /gene="ldtA"
                     /locus_tag="OO564_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6654318.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6807..7886)
                     /gene="cobT"
                     /locus_tag="OO564_003306"
     CDS             complement(6807..7886)
                     /gene="cobT"
                     /locus_tag="OO564_003306"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6654319.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7898..8630)
                     /gene="cobS"
                     /locus_tag="OO564_003307"
                     /pseudo
     CDS             complement(7898..8630)
                     /gene="cobS"
                     /locus_tag="OO564_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8627..9172)
                     /gene="cobU"
                     /locus_tag="OO564_003308"
     CDS             complement(8627..9172)
                     /gene="cobU"
                     /locus_tag="OO564_003308"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6654320.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9799..10062)
                     /locus_tag="OO564_003309"
                     /pseudo
     CDS             complement(9799..10062)
                     /locus_tag="OO564_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2860 a         2561 c         2134 g         2510 t
ORIGIN      
        1 tggcaaatag cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat
       61 tatcgtctta atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa
      121 aaatcccgcc aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata
      181 tttttatttc taatagacga gatttttcct gttatgatat aatatgccga attaacacat
      241 gttaacgatt taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa
      301 atgggccaat attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc
      361 aaaattcggt gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa
      421 cacatcttta aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat
      481 tgataaagca attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta
      541 tgaaggcttt ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa
      601 ccgtacgatt gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc
      661 aggttctgtc agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc
      721 tgaccatatt tttgaaattt tgcttgacgc tgaagttgat gttcgtgatg taactgaaga
      781 agaaggtaat attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct
      841 aaaagcagct ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga
      901 agttgagctt tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga
      961 tgacgacgat gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga
     1021 aatctgtctt tatggcagat ttctttaatc tcatataatt cttataaaaa atataatgtt
     1081 taactcgtca tattgcttat aaaaaatccc cgttcccaga gaaataatat ttattaaaat
     1141 ggcaggtctt ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat
     1201 aaagacgcga cagctacatc ccgcataagg catttttctc tttatctttg tacggtactt
     1261 catggaacag aatttttgac cttgtgaatc gtgatgtctg ttggggaggg gcaatttgct
     1321 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact
     1381 tttttacaaa gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag
     1441 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     1501 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     1561 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     1621 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg
     1681 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     1741 cgcacagccc agtaggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     1801 attccctgca cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac
     1861 atggcgtaac gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac
     1921 caaataggca taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt
     1981 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     2041 gccgttaatc caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct
     2101 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt
     2161 ccgggtaagt tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg
     2221 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     2281 tcgacactcg ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     2341 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     2401 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg
     2461 agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt
     2521 taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag
     2581 tgatggcagc ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt
     2641 taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa
     2701 ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca
     2761 ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa
     2821 gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc
     2881 cggccatcgc atcttttccc agccagctga ccagaaaagt gctcaggccc cccatcaaca
     2941 ggacacagac gttctccatg aagataggaa cagcaagcgg ggttatctcg cgccagaaca
     3001 acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag
     3061 cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg
     3121 agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa
     3181 ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt
     3241 tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga
     3301 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc
     3361 cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca
     3421 tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc
     3481 aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga
     3541 aaagatgccg cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt
     3601 cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct
     3661 gtgcacttaa tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg
     3721 agcgccccgt aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg
     3781 tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac
     3841 aaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa
     3901 tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct
     3961 cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac
     4021 gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt
     4081 ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg
     4141 ctttgcccgg ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc
     4201 caagttcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca
     4261 tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt
     4321 gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat
     4381 gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct
     4441 caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc
     4501 tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga
     4561 gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga
     4621 acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag
     4681 cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc
     4741 ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc
     4801 aatagcatta acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc
     4861 ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc
     4921 catatcgagt tggtgattta tcagtttttc gttaagcact gcgccactat tttcatgaag
     4981 atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga
     5041 tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac
     5101 gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata
     5161 gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg
     5221 atttaactca ccttccagta tggcaacctg ctggctgagc gctggttgtg cgatatgcaa
     5281 tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg
     5341 tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca
     5401 accagtatgc aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg
     5461 tcaaataaga gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc
     5521 tgccctataa aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc
     5581 accactttgt tgaaattggc agcaaacaaa caaaaaaatg catttcaccc tttgacatca
     5641 ccatgcactg ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag
     5701 aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca
     5761 aaaaagcctg ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct
     5821 gtcttgaacc agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg
     5881 cgcgatttac atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca
     5941 atggcacttt tcggtcagac tcatattctg cacgattgcg tgacagcggc tcatgaactt
     6001 ccagccactt tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta
     6061 cacgtgtgcc tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa
     6121 tacagccctg acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat
     6181 acaacctgcc aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa
     6241 atgcgggcaa actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc
     6301 aggttggtgc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc
     6361 cagcctggcc gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat
     6421 aaagacgcat ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca
     6481 gttgctgcgg aatggtgagt tgtgagccag gcttcggtaa aaaaacatca gcgcccggat
     6541 tcgcttccag catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct
     6601 gagtattgtg atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg
     6661 gaggtaatgg ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc
     6721 aaagaatatt tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag
     6781 tatagctttt atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt
     6841 agaacaatat tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg
     6901 atgatggaca tcgccagagc tgcaccactc ccctcaccta aacgcatatc catattgaga
     6961 taaggctcca gccccaaatg cgagagcgct atacgcgcgc ctttttctgc cgacaagtga
     7021 gaaggaatga gatacggttt gattgcagga gacatctggc aggctgcgag cgcagcagca
     7081 taagaaagaa atccatccag caacacgggt aaaccgcagg aagcagcacc taacatcact
     7141 ccagctattc cgaccaaatc aaatccaccc acttttgcca ggacatcaac accatcctga
     7201 ggatttggtt gattcaacgt aatcgcccga cgcacaacat caattttatt agccagttta
     7261 tctgtcggca ggtttgcgcc aatcccaacc acttcttcag gatcccggcc agtgattgtg
     7321 ctgactattg ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc
     7381 gtgacaccgt ttttcgccag ctcccgcgta taacatatga cgtccaaaag caacttttca
     7441 gcctgacggc gactcattgc cggagctgaa gcaatattgc cgctacctcg tgcgacacgc
     7501 atgttgataa gcccggggat aggctcagca gtatcaatac caacatcaat tacgtggacg
     7561 ttagcgcccg cttgtgctgc cagcacacac acgccggttg ttccacgggt catattttca
     7621 gcctgtatgg ctgtcacttc ttttggagaa atagcgaccc cttcctccca gacgccgtga
     7681 tcggcacaca taaccagtac cgcttttttg cccacatgcg gtatgccatt cacccccggc
     7741 attcctgcca gttgtatggc aagcgcctcc agctttccta ggctaccaac aggtttgagt
     7801 aacccgtcaa catgccgttg tgcacgcgac atagcggcag aattgatggc tgggatcgta
     7861 ttcagtaaat cggcaattat ttgcatgtca tgtctcttta tagcagtgcc agcaggaaga
     7921 ccagttcacc aagctctatg gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac
     7981 gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac
     8041 catgcatacc cagcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg
     8101 tttgtcgcgc caatgaacac attgccaagc ccttcttccc gcgcataacg atgacgatac
     8161 atcaataatg cggcagtccc gcggctgacc gcacatgctg ccgccagcga tgcaaggatc
     8221 ggttcgccac gcagagccaa ctcgcttaac accagaatct ttgccagtac cacaaaaatc
     8281 aatgccagac cgccgtgggt gcctaaacgg ctatcacgca tgatttccaa catgcgatcg
     8341 cggctacgtg cagaaaatac gccatcacag gtatcagcga ggccgtccag atggaattcc
     8401 ccggtcatca gcgccagcac aagtacgcta aacagtgccg ccagtggtgc gccacaccat
     8461 gcctgcagca ccatgaagac cagcccgcta atcgcgccaa gcaataatcc aatcaaagga
     8521 aaagtaataa taccgcgaga ataatgctcg aaatccagtc cctgggacca gcgacgcggt
     8581 acaggcagac gcgtaataaa tgagagcatc gcccaaaata atttgctcat ttgattttta
     8641 ctccaatacc cgaaaccacc agccatactt catttgccgc agcggccaac tgctgattta
     8701 cccgcccggc aatatcacga aaatgtcgtg ccagacgact ctccggcaca atccccattc
     8761 ccacttcgtt agtcactaat acaacctttg cggggcaacg ttggcaggca gcaatcaacg
     8821 actgaatctc agcattaatc gcctgttcca tcgcctgata atcccattca tcagggtctt
     8881 tatcgccgcc ataatcaaac aacagattag tcaccattgt ggtaacgcat tccagcaaca
     8941 cagcctcatt cgggttaatg tctgcatgaa ttaattcatc aagatgttgc cagcgctcca
     9001 ctgtgcgcca gtgctccggg cggctttgcc gatgatgttc tatccgtgca gccatctcat
     9061 catcaaggat ttgcgaggta gcgatataca gaacctgtga agagtcccca ataagcacct
     9121 ctgcatggcg actcttcccg ctccatgcgc ctcccgtcac caaaatcatc ataccgactc
     9181 ctgattcaaa tgtatgatgg tatacattga acagagcaat ggatccccca ccgttgctga
     9241 ccgcaaaaca tttacgcgat taatactgcg cgtaatataa ttaaaaatcc agcattctca
     9301 atccattacg aaataagatg gcattacgga atagccagac tttctcgtgt taacgtccga
     9361 caaaatgcgt cattgacagc ggtttctgtg gattgacaca tttcattcag ataatgaatt
     9421 aatgctactg ccggaccaac cagcaaacgg gcattgtgtt ctgaaatcca ggctattgat
     9481 tcaacgtcag cgacagatct gcgctgtaaa ataacttgta aaacaacgca atagaattat
     9541 ccggaatggt ggcgactatg cactagaaca ccacaatttc gctctctcgg taaggcactg
     9601 tcacagcatt caacagaatg tgacttgcca gatttattag cgccaccaga tgtttaaccg
     9661 ggtaaccacg acccagtcgg ggatatattc catcttctcg ctgacatcat caagaataaa
     9721 aaggtttatc acactcagag caacgttctt cagcaggcgg gataattctt caacatttaa
     9781 aagatgtgtt attaaaggca tctcaaattg gacagggctg ccggttttct gttgcaacac
     9841 cgttgctctg cttgagacag aactgccata tgcagcatct gatactttga gtttgaccaa
     9901 atcacagccc cgtagcttac tgtccaaagc catgttgaac agagctagat cgcgtgtttt
     9961 accttccaac tcaagtctaa tccggatccc ccagatatga gatatctgaa gtggcctttt
    10021 ttgaccgatg gtacggtctt tattccacgg tgacgttttc atatt
//
LOCUS       DAJTYI010000134         9387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYI010000134 DAJTYI010000000
VERSION     DAJTYI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1005)
                     /locus_tag="OO564_003310"
     CDS             complement(<1..1005)
                     /locus_tag="OO564_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6654321.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     T"
     gene            complement(1204..1288)
                     /locus_tag="OO564_003311"
     tRNA            complement(1204..1288)
                     /locus_tag="OO564_003311"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1252..1254),aa:Leu,seq:caa)
     gene            1484..2503
                     /gene="ahr"
                     /locus_tag="OO564_003312"
     CDS             1484..2503
                     /gene="ahr"
                     /locus_tag="OO564_003312"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6654322.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2632..4134
                     /locus_tag="OO564_003313"
     CDS             2632..4134
                     /locus_tag="OO564_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6654323.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4253..5335)
                     /gene="lptG"
                     /locus_tag="OO564_003314"
     CDS             complement(4253..5335)
                     /gene="lptG"
                     /locus_tag="OO564_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6654324.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5335..6435)
                     /gene="lptF"
                     /locus_tag="OO564_003315"
     CDS             complement(5335..6435)
                     /gene="lptF"
                     /locus_tag="OO564_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6654325.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6702..8213
                     /gene="pepA"
                     /locus_tag="OO564_003316"
     CDS             6702..8213
                     /gene="pepA"
                     /locus_tag="OO564_003316"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6654326.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8347..8790
                     /gene="holC"
                     /locus_tag="OO564_003317"
     CDS             8347..8790
                     /gene="holC"
                     /locus_tag="OO564_003317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6654327.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8790..>9387
                     /locus_tag="OO564_003318"
     CDS             8790..>9387
                     /locus_tag="OO564_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCR6654328.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWH"
BASE COUNT         2298 a         2433 c         2542 g         2114 t
ORIGIN      
        1 cgtgtcatac cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg
       61 tggatcgtga tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc
      121 caaaatactt agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc
      181 acgctgagaa tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc
      241 cgtttcaaaa tctacttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac
      301 caataaagtg agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg
      361 atgaagtaat tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt
      421 agcaaccgca cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc
      481 aaagcgcata atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga
      541 tgactcgact gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg
      601 cttaccgata gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga
      661 tgccgaccac ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc
      721 ctcttgttcc tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc
      781 taaaagctta cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta
      841 tttccccaat gcaagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt
      901 ggagccgtta gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt
      961 tgcttcaggc ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc
     1021 aagggttctg tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca
     1081 tcagtaagtt gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag
     1141 ccttgcggat actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc
     1201 cgatggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa
     1261 ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt
     1321 atacctgtcg cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa
     1381 ttttcagcat tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc
     1441 catgctctac acttcccaaa caacaccaga gaaggaccca aaaatgtcga tgataaaaag
     1501 ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct
     1561 gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc
     1621 gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat
     1681 tgggcgcgtg gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg
     1741 tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa
     1801 tcagatcaac tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga
     1861 gaagttgcgt gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc
     1921 cgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat
     1981 cactgctacc agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa
     2041 acttctgcat gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca
     2101 ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa
     2161 agccctgtcg gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca
     2221 gccttatttt gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac
     2281 gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc
     2341 taccggcacg ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc
     2401 gccgacaacc gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga
     2461 tggtaaagcg cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct
     2521 gtcgcccggc acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga
     2581 agagtgcaca gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa
     2641 cccctgttaa ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac
     2701 cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg
     2761 gcagagtcat tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg
     2821 accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc
     2881 ggcgtcaatg actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag
     2941 aaaggccaca cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg
     3001 ttgaatctca acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat
     3061 cagggattgt tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat
     3121 aacgccaaat ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc
     3181 cagcgcggat tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg
     3241 ctggatatca aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc
     3301 agcgccgaga aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt
     3361 tcagagttgt atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt
     3421 ttcttcgacg aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt
     3481 gagcaggtga taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac
     3541 ccgtctgata ttccggataa tgtgctcggc cagctcggta atcgtgttca gcacgctttg
     3601 cgagctttta cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc
     3661 aatccggcat ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc
     3721 tcgtttctcg atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct
     3781 tgttcgcgaa tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg
     3841 gtgtatggca aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa
     3901 ggctttcagg ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg
     3961 gtggatgacg gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc
     4021 ggcgggaaga aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg
     4081 aatcagattg tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga
     4141 cgtgatctgt gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact
     4201 cgtatttgac tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt
     4261 ttctcattaa cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga
     4321 tgatcggcgg gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga
     4381 agacaaaacc gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg
     4441 ggccaaagat aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga
     4501 ttttgctcca catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact
     4561 tcacgtagtt gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca
     4621 gtttgtctgg cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct
     4681 gtttcggatt ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg
     4741 ggtcaaactt cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg
     4801 cataaatact gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt
     4861 tgttgccatc tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg
     4921 cctgcgcacg gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg
     4981 ccatcgtcag caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac
     5041 gggtaaaacc agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac
     5101 caagcaacgc cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc
     5161 tcagcaaggt atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca
     5221 gctgatcgac aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga
     5281 tggtggtgaa aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac
     5341 cgctccttta cgcgaaaaac tggcgcgcag gcggcggatc ggcacggtgt cccaaaggtt
     5401 gagaacaatc gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc
     5461 cagcttacct ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata
     5521 cagcagcatg gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa
     5581 cggtacaacc ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag
     5641 ttctgcgcga gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt
     5701 atcgttcggg tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc
     5761 cgtaatgcgg aaatcacgta acagtgcagt gccttcgaag cgtgttccct ggttgagagt
     5821 gacgacctgg gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga
     5881 aggacgtgca ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc
     5941 gtcaacgctt tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg
     6001 cgccagcgcc gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg
     6061 cgatgaccac ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc
     6121 aaggatcatt gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt
     6181 aatttcactt tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa
     6241 tggcaggata agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt
     6301 cgccggaata tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa
     6361 gatcaaaagc aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata
     6421 tcttatgatt atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt
     6481 gtttcatggc ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt
     6541 atactcaacg gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta
     6601 agattaacac gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct
     6661 gtagccaccg ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa
     6721 gcggtagccc ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc
     6781 gcctttctcc gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc
     6841 tacgccgggg cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga
     6901 atgtactttc cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc
     6961 gtcagtacaa gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg
     7021 aagcggtctg ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc
     7081 gtcaggctgt cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata
     7141 agagcgaacc gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac
     7201 tgaccagcgg tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag
     7261 cgaaagatct cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg
     7321 cgcgccagct ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc
     7381 agatgaaaga gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat
     7441 cgctgatgtc ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc
     7501 tggtgggtaa aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca
     7561 tggatgagat gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg
     7621 tcgcggagct gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc
     7681 ctggcgggcg tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg
     7741 aagtgctgaa caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg
     7801 aacgttttga gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg
     7861 cgctgggtca tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga
     7921 ttgccgcgtc tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc
     7981 aggaacagct ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg
     8041 cgattaccgc aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg
     8101 atattgcagg aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag
     8161 cgttgctggc acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg
     8221 ttttaccctc accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa
     8281 ttcattgctc atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc
     8341 ccatatatga aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta
     8401 agcgccgttg agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc
     8461 gtactcatcg cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg
     8521 cgtccggcag aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcggtgcg
     8581 ccggtggaga tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt
     8641 ctgcgaacaa gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct
     8701 tatgaagatt ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt
     8761 ttcaacctga atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg
     8821 aacagccgct ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa
     8881 gccaggaaag tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg
     8941 gtcacgcctt ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca
     9001 aaaacaccct gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg
     9061 agcgcaagat tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca
     9121 tcgacaaaat ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc
     9181 gtctcggcaa ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca
     9241 atgcggtgaa agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc
     9301 gcctggtaaa ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc
     9361 gcgaatcgaa aggttcgatg tggcaca
//
LOCUS       DAJTYI010000135         9321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYI010000135 DAJTYI010000000
VERSION     DAJTYI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..955)
                     /gene="aroA"
                     /locus_tag="OO564_003319"
     CDS             complement(<1..955)
                     /gene="aroA"
                     /locus_tag="OO564_003319"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6654329.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTI"
     gene            complement(1026..2114)
                     /gene="serC"
                     /locus_tag="OO564_003320"
     CDS             complement(1026..2114)
                     /gene="serC"
                     /locus_tag="OO564_003320"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6654330.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2313..2941)
                     /locus_tag="OO564_003321"
                     /pseudo
     CDS             complement(2313..2941)
                     /locus_tag="OO564_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3071..4831
                     /locus_tag="OO564_003322"
     CDS             3071..4831
                     /locus_tag="OO564_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6654331.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5237..6094
                     /gene="focA"
                     /locus_tag="OO564_003323"
     CDS             5237..6094
                     /gene="focA"
                     /locus_tag="OO564_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6654332.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6149..8431
                     /gene="pflB"
                     /locus_tag="OO564_003324"
     CDS             6149..8431
                     /gene="pflB"
                     /locus_tag="OO564_003324"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6654333.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            8623..>9321
                     /gene="pflA"
                     /locus_tag="OO564_003325"
     CDS             8623..>9321
                     /gene="pflA"
                     /locus_tag="OO564_003325"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6654334.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERV"
BASE COUNT         2400 a         2338 c         2235 g         2348 t
ORIGIN      
        1 caatggtcat cgccgcatca ggaatatggt tcatatccat atcaatagcg ttcagttcac
       61 cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt ttttccagca
      121 catcagcaaa gcgaatatca ccctgcatac tgttacgtcc aataccggtc acttttacag
      181 tgccgccttt gattgctgct gctgccagga agtaagaagc cgaagatgca tcgccttcga
      241 ccaaataagt acccggagac tgataagact gcccgccttt tacgacaaat tgttgatagt
      301 gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagtgtgatg tcgatataag
      361 gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca agaggcgcag
      421 tcattaacag tgcggtgagg aattggctgg aaacggagcc atcaacgtca acgttgccgc
      481 cagtaaagcc gccctgtaaa cgcaacggcg gataattttc ttgttccagg taagtgatct
      541 tcgccccgcc caggcgcagc gcatccacca gatgaccaat cgggcgctct ttcatacgcg
      601 gctcaccggt cagcacaata tcattgctat ccagacaaag agctgccgcc agcggacgca
      661 ttgccgttcc ggcgttaccg aggaacaact ccagggcacc ttctgcgtgt aatggaccgc
      721 cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt aaccctaacg
      781 ctgttaatgc attcagcata tggcgcacgt cgtcgctatc cagcagattg gttaatactg
      841 ttttgccgtg tgctaatgcc gcaagcaata aagcgcggtt agaaacgctc ttggaaccgg
      901 gcagattaat agtgccatcg acacgagcga tgggttgtaa cgtcagggat tccatgaact
      961 caactctcta caacagaaat aaaaacccca caggctggct gtggggatta agcaaaattt
     1021 cggcattaac cgtgacggcg ttcgaactca accatgaagt ctgtcagcgc tttaacgcct
     1081 tccagcggca tggcattata aatagacgcg cgcattccgc cgaccacacg gtgtcctttc
     1141 agtgcatgaa ggcctgcagc aaaagactct tcaaggaaca atttgtcaag cgcactgtcc
     1201 gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacatc attgcggtag
     1261 aaatcgctgt tatcaatcac cccatatagc agttctgctt tttgctgatt gattttatcc
     1321 atttcagcta caccgccgtt cgctttcagc catttaaaga ccagaccaga tagataccag
     1381 gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt tgaggatgga ataatccaga
     1441 atcgacggac acgcgatatt cgctttgccc agcaaatctt cacgaacgat gacgattgtc
     1501 aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa ttacaccata acggctgacg
     1561 tcaatcggac gggaaagaat ggttgaagag aagtcggcgg cgaccaccac atctttgccg
     1621 aagtctggcg tctcgtcgat ggcgataccg tcgatggttt cattcgggca gtaatgcata
     1681 taagccgcat tatcagagag ttgccattca cgcattggct taaccgcgcg cagaccatca
     1741 acagtcactt tggcgtcaaa gacattaggc gtgcagtatt ttttcgcttc tttaatggca
     1801 cttgccgccc agtaaccggc atcaacataa tctgcggtgg ttttatcacc gagaatattc
     1861 agcggtaccg cagcaaactg accgcgacca ccgccatggc agaataatac cttgtagttg
     1921 gaggggacat taagaagatc gcgaaaatcc ttctcggctt cctctgcaac ctgaatgaac
     1981 tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca gtcgcgcagt
     2041 tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga actaaaattg
     2101 aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgcgg tcaatcgatt
     2161 gcgaccacag gtaatacatt attcctgtgg ctatcggttt tatcattcag tgacacacat
     2221 cgcaatgagt aaaaccgttg agagcaaaaa tgtggcttgc atcacacaaa agaatctctc
     2281 ggattattag caaaaagccg atgtttttac tgtcaggtca cccgttttgc ccgacttgcc
     2341 ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc gttcatatga
     2401 atgatttcac tacaacgaat gcaggcatag tccgccgact ccggcaccac tttgtaacgc
     2461 tgcgtacaat cactgggtaa aataagtaaa cccggtttca ccttgtcatc ttcaaagaaa
     2521 tagacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc cagctgctcc
     2581 acgccacgca atcgtagctc cattaaagaa ctcaaattcc gtcatgttgg agttattgag
     2641 tttcgaccag gccagctcgc catcacatta ccaggcggta taacaacgcc agagtgataa
     2701 agacgataag taccggcacc atcggcacat catcataaaa cgccacatct cccgccgctg
     2761 aacccagcgt gagaatgatt aacacttcaa acagcgacat ctgtcgcaca ccgcgtcttc
     2821 cggtcatttt gaggaacaaa aaaaccagta caaaggtata aagactacgt agtgcaactt
     2881 cgccaaggaa atcaaaaggc actttatcaa atgccatacg gtggagatcg aacgccttca
     2941 tttttatgct cttaacgtgg tttatccgct aaaagcatag tcaacagttc atcggcatgg
     3001 cggcggtgat agcgcctttg agataaaacc cgtattattg cgcgctttcc gtacgactaa
     3061 agtgattttc atgacgcaaa catttatccc cggcaaagat gccgctctgg aagattccat
     3121 cgctcgcttc cagcaaaaac tttcagacct cggctttcag attgaagagg cctcctggct
     3181 gaatcccgtg cctaacgtct ggtctgtaca tattcgcgac aaagagtgcg cactgtgttt
     3241 taccaacggt aaaggcgcaa ccaagaaagc ggcgctggct tctgcactcg gtgaatattt
     3301 cgagcgtctc tcaaccaact acttttttgc tgatttctgg ctgggcgaaa ccatcgccaa
     3361 cggtccattt gtgcattatc ccaacgaaaa atggttccca ctgagcgaaa atgacgatgt
     3421 gccagaaggg ctgctcgatg accgtctgcg cgcattttac gatccggaga atgaattgac
     3481 cggcagtatg ctgattgacc tacaatccgg taacgaagat cgtggtattt gcggtctgcc
     3541 gtttacgcgc cagtccgaca atcagaccgt ttatattccg atgaatatca ttggtaacct
     3601 gtacgtttct aacggtatgt ccgctggcaa tacccgcaac gaagcacgcg ttcaggggtt
     3661 gtccgaagtt ttcgaacgct acgtgaaaaa ccgcattatt gctgaaagca tcagcttgcc
     3721 ggaaatcccg gcagacgtgc tggcgcgtta cccagcggtg gttgaagcga tcgaaacact
     3781 ggaaacagaa ggtttcccga tcttcgcata tgatggttcc cttggcggcc agtatccggt
     3841 gatttgcgtg gtactgttta atcctgcaaa cggcacctgc tttgcctctt tcggtgcgca
     3901 tcctgatttt ggcgtagcac tggaacgtac cgtgaccgag ctgctgcaag gtcgtggcct
     3961 gaaagatttg gatgtgttta ctccgccaac cttcgatgat gaagaagtcg ctgaacatac
     4021 caacctcgaa acgcacttta tcgattccag cggtttaatc tcctgggacc tgttcaagca
     4081 ggatgccgat tatccgtttg tggactggaa tttctccggc accacggaag aagagtttgc
     4141 tacgctgatg gctatcttca acaaagaaga taaagaagtt tatattgccg attacgagca
     4201 tctgggcgtt tatgcttgcc gtattatcgt gcctggcatg tccgatattt atccggctga
     4261 agatctgtgg ctcgcgaata acagtatggg cagccattta cgtgaaacga ttctttcgct
     4321 accaggcagc gagtgggaaa aagaagatta cctgaacctc atcgagcaac tggatgaaga
     4381 aggttttgat gactttaccc gcgtgcgtga gctgttgggt ctggcgaccg ggtcggataa
     4441 cggttggtac accctgcgta tcggtgaatt aaaagccatg ctggcgctgg ctggtggcga
     4501 tctggaacag gctctggtct ggaccgaatg gacgatggag tttaactcat cagtatttag
     4561 tccggaacgc gccaactatt atcgctgcct gcaaacgttg ttattactgg cacaggaaga
     4621 agatcgccag ccgctgcaat atctgaatgc gtttgttcgc atgtacggcg cagatgccgt
     4681 agaagccgcc agtgcggcaa tgagcggcga agcggcgttt tacggcctgc aaccggtaga
     4741 tagcgatctg cacgcgtttg ctgcacatca gtcgttgttg aaggcctacg aaaagctgca
     4801 acgcgccaaa gcagcattct gggcaaaata aaatcaaata gcctacgcaa tgtaggctta
     4861 atgattagtc tgagttatat tacggggcgt ttttttaatg ccccgcttta catatatttg
     4921 cattaataaa ataattgtaa ttataaggtt aaatatcggt aatttgtatt taataaatac
     4981 gatcgatatt gttactttat tcgcctgatg ctccctttta attaactgtt ttagcggagg
     5041 atgcggaaaa aattcaactc atttgttaat ttttaaaatt tatttttatt tggataatca
     5101 aatagttact ccgtatttgc ataaaaacca tgcgagttac gggcctataa gccaggcgag
     5161 atatgatcta tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa
     5221 taaagagaga gttagtgtga aagctgacaa cccttttgat cttttacttc ctgctgcaat
     5281 ggccaaagtg gccgaagagg cgggtgtcta taaagcaacg aaacatccgc ttaagacttt
     5341 ctatctggcg attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc
     5401 aaccactggc acaggcacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt
     5461 ctctctgggg ctgattcttt gtgttgtctg cggagccgat ctctttactt ccacagtgtt
     5521 gattgttgtt gctaaggcga gtgggcgcat cacctggggt cagttggcta aaaactggct
     5581 aaatgtctat tttggcaatc tggtcggcgc actgctgttt gtacttttaa tgtggctttc
     5641 cggcgagtat atgaccgcaa atggtcaatg gggactaaac gtcctacaaa ccgccgacca
     5701 caaagtgcac catactttta ttgaggccgt ctgtcttggt atcctggcaa acctgatggt
     5761 atgtctggca gtatggatga gttattctgg ccgcagcctg atggacaaag cgttcattat
     5821 ggtgctgccg gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt
     5881 tatgatcccg atgggtattg taatccgcga cttcgcatct ccggaattct ggaccgctgt
     5941 cggttctgca ccggaaaatt tttctcacct gaccgtgatg aacttcatca ctgataacct
     6001 gatcccggtt acgatcggta atattatcgg cggtggtttg ttggttgggt tgacatactg
     6061 ggtcatttac ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg cagtaaataa
     6121 aaaatccact taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc
     6181 ctgggaaggt tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca
     6241 gaaaaactac actccgtacg agggtgacga gtccttcctg gctggcgcta ctgaagcgac
     6301 caccaccctg tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc
     6361 agttgatttt gacaccgctg ttgcttccac catcacctct cacgacgctg gctacatcaa
     6421 taaagcgttg gaaaaagttg ttggtctgca gactgaagct ccgctgaaac gtgctcttat
     6481 cccgttcggt ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga
     6541 cccgatgatc aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtattcga
     6601 cgtttacact ccggacatcc tgcgttgccg taaatccggt gttctgaccg gtctgccaga
     6661 tgcttatggc cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga
     6721 ctacctgatg aaagacaaat acgctcagtt cacctctctg caggctgatc tggaaaacgg
     6781 cgtaaacctg gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct
     6841 gggtcagatg aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggcaaccaa
     6901 cgctcaggaa gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa
     6961 cggtgctgca atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga
     7021 cctgaaagct ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat
     7081 gaaactgcgt atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga
     7141 cccaatctgg gcaaccgaat ctatcggtgg tatgggcctc gatggtcgta ccctggttac
     7201 caaaaacagc ttccgtttcc tgaacaccct gtacactatg ggtccgtctc cggaaccgaa
     7261 catgaccatt ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt
     7321 gtccatcgac acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa
     7381 caacgatgac tacgctatcg cttgctgcgt aagcccgatg atcgttggta aacaaatgca
     7441 gttcttcggt gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt
     7501 tgacgaaaaa ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag gcgatctcct
     7561 gagctacgat gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta
     7621 catcactgca ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct
     7681 gatggcgctg cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggtctgtc
     7741 cgttgctgct gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga
     7801 agacggtctg gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaatga
     7861 tccgcgtgta gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa
     7921 actgcacacc taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt
     7981 tgtgtatggt aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg
     8041 accgggtgct aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt ctctgacttc
     8101 cgttgctaaa ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt
     8161 tccgaacgca ctgggtaaag acgacgaagt tcgtaagacc aacctggctg gtctgatgga
     8221 tggttacttc caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat
     8281 gaaccgtgaa atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat
     8341 ccgtgtatct ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt
     8401 tattactcgt accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa
     8461 agcgtacaat aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc
     8521 agctatctat actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca
     8581 ccaacacggc ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc
     8641 attcactcct ttgaatcctg tggaaccgta gacggcccag gtattcgctt tatcaccttt
     8701 ttccagggct gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggacacgcat
     8761 ggcggtaaag aagttaccgt tgaagatttg atgaaggaag tggtgaccta tcgccacttt
     8821 atgaacgctt ccggcggcgg cgttaccgca tccggcggtg aagcaatcct gcaagctgag
     8881 tttgttcgtg actggttccg cgcctgtaaa aaagaaggca ttcatacctg tctggatacc
     8941 aacggttttg ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg
     9001 gtaatgctcg atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttcc
     9061 aaccaccgca cgctggagtt cgctaaatat ctggcgaaca aaaatgtgaa ggtgtggatc
     9121 cgctacgttg ttgtcccagg ctggtctgac gatgacgatt cggcgcatcg cctcggtgaa
     9181 tttacccgtg atatgggtaa cgttgagaaa atcgaacttc tcccctacca cgaactgggc
     9241 aaacacaaat gggtggcgat gggtgaagaa tacaaactcg atggtgttaa accaccgaag
     9301 aaagagacca tggaacgcgt g
//
LOCUS       DAJTYI010000136         9278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYI010000136 DAJTYI010000000
VERSION     DAJTYI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..171
                     /locus_tag="OO564_003326"
     CDS             <1..171
                     /locus_tag="OO564_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6654335.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSKNKESTNDV"
     gene            521..883
                     /locus_tag="OO564_003327"
     CDS             521..883
                     /locus_tag="OO564_003327"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6654336.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            880..1797
                     /locus_tag="OO564_003328"
     CDS             880..1797
                     /locus_tag="OO564_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6654337.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1810..3075
                     /locus_tag="OO564_003329"
     CDS             1810..3075
                     /locus_tag="OO564_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6654338.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3142..3765)
                     /locus_tag="OO564_003330"
     CDS             complement(3142..3765)
                     /locus_tag="OO564_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6654339.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4516..4854)
                     /locus_tag="OO564_003331"
                     /pseudo
     CDS             complement(4516..4854)
                     /locus_tag="OO564_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4976..5236
                     /locus_tag="OO564_003332"
     CDS             4976..5236
                     /locus_tag="OO564_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6654340.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5254..7989)
                     /locus_tag="OO564_003333"
                     /pseudo
     CDS             complement(5254..7989)
                     /locus_tag="OO564_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7990..>9278)
                     /locus_tag="OO564_003334"
     CDS             complement(7990..>9278)
                     /locus_tag="OO564_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6654341.1"
                     /translation="GGVNSNAPNVNDIGNTLQLIRQNNDIERSGANNVGLTALQGLSG
                     IAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQIESVRKGMGFIDEDQ
                     RNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVAQMYQQNPRQFGEFVD
                     HLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRI
                     NLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGI
                     NTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQA
                     MRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNG
                     IKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         2416 a         2137 c         1990 g         2735 t
ORIGIN      
        1 tcagtgttac aggaaatggg aggctgggag tctatcgaaa tggttcgtcg atatgctcac
       61 cttgcaccta atcaccttac cgaacacgca cggcaaatag actcgatcct gaacccatcg
      121 gtcccaaatt tgtcccagtc aaaaaataag gaaagtacta atgatgtgta acttattgat
      181 ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa tctgtagcac
      241 caatcataac tatctgtttt agcaagcatt aaccgcattc actaagatat agttgatggc
      301 acaaacagaa agttgatgaa tgatgttgcc atatatatgt cacaaatacg gcacaacgat
      361 cttcaaacat atagccacac attcacagaa gagcacaaag ccttgcaatc cagtgcaaag
      421 atttgtgtgc cagagttttt ctcatcaact accgcaagta tcgatcgatt gagacttgga
      481 tgatagactt catgcctttg attattagct gatagaagaa atgttaaagc tatttgcaaa
      541 gtacacatct ataggcgtgc tgaacacact tatacactgg gtggtttttg gtgtatgtat
      601 ctatgccgcg cataccaatc aggctatggc aaacttcgca ggtttcgttg tggctgtgag
      661 ttttagcttc tttgcgaatg caaaattcac attcaaagca tcaactacaa cgatgcgcta
      721 catgttatat gtcgggttca tgggaacact gagtgctact gttggatggg ttgctgatag
      781 atgttcactt cctccaattg tcactcttgt taccttctcc gccatcagct tggtgtgcgg
      841 tttcgtctat tcaaagttca ttgtctttag ggatgcgaaa tgaagatatc tcttgtcgtt
      901 cctgtcttca atgaagaaga agcgatacca attttttata aaacggtacg tgaattcgaa
      961 gagttgaagc catatgaagt agaaattgtt ttcataaatg acggaagcaa agacgctacg
     1021 gagtcaatca ttaacgctct ggctgtttct gatccgctag ttgttccgct gtcatttaca
     1081 cgcaactttg gtaaagaacc agcattgttt gcagggttag accatacaac cggcgatgcc
     1141 gtgattccaa ttgatgttga cctgcaagac ccgattgagg ttattcctca tcttattgaa
     1201 aagtggcagg caggtgctga catggtgctt gctaaacgtt cagaccgctc aactgatgga
     1261 cgactgaaac gtaagacagc tgagtggttc tataaattac acaacaaaat aagcacccca
     1321 aaaatagagg aaaatgtcgg agattttcga ctcatgtctc gtgaggttgt ggagaacatt
     1381 aaactattgc ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt tggtggtcag
     1441 acagatgtcg ttgaatatgt acgcacagag cgtgttgctg gcatctcaaa atttaatggc
     1501 tggaaattat ggaatctggc actggaaggt atcacaagtt tttcaacctt tcctcttcgc
     1561 gtatggactt atataggctt gtttgttgca agcatttcat ttttatatgg tgcatggatg
     1621 attatagaca ccattgtctt tggtaaccca gtacgcgggt atccctccat gcttgtatca
     1681 atacttttcc tgggtggagt gcaactgatc gggattggtg ttctcggtga atatataggt
     1741 agaatttatt tagaaacaaa atcaagacca cggtacttaa ttaaaagcag aaaataacag
     1801 gatgatctta tgaagatatt cataatgcgc aattggcaca agatatcaat ttttatactt
     1861 gcatttactt tgatttggct taggcgcatt gacattttga caaatgccca gttttgggct
     1921 gaggatgcag ttttttggta taaagatgca tacgaacaag gttttttcag ttcactcata
     1981 accccaagaa atggttattt tcaaacagta tcaacactca ttgttggtgc gacgacattt
     2041 attaatccaa tttacgcacc attattatca aacttttttg gaataataat tagagctata
     2101 attatatggt tcttatttac cgatagattt aaattcctaa gcaccacatc aaaaatcttc
     2161 atctctgtat atttaatttg catgcccggg cttgatgaag tccaagccaa tataacaaat
     2221 gctcactggt atttatcatt gtatgttgca atgataataa tatctgaaga atccaagtca
     2281 aaattatgga aagcacatga tttatttttt atagtattat ctggattgag cggtccattt
     2341 attatattca taattgcatc atctgttttt aaatatttac acttatcaaa aggagtaata
     2401 agcataagag gtttatactt attttatact cgactgccat atttggcaat gatcttgtgt
     2461 gggctgatac aggcaacatc aataattctt acatttaatg gaacaagatc acatgctcca
     2521 ttaggattca gttttgatgt tatgtcatct ataatatcat ctaatgtatt tcttttttca
     2581 tttgtccctt ggaatatcgc taatgctgga tgggataatc accttttatc gtacacacta
     2641 tcaactttag taattacatt atgtgttttc atttatatta aaggaaattg gcaaatgaaa
     2701 gtgtttgcca cccttccaat acttattgtc gcattttcta tggcaaaacc tcaactggca
     2761 gattctgttc ctcaactgcc aacacttgca accggaaacg ggtcaaggta ttttgtaaat
     2821 attcatattg caattttttc attaatatgc gtttgtttgt ttcaatgcat aaagaataaa
     2881 accttaaaaa tattctttaa aatctatgtt tcagtcatcc ttattgcaat gataaaatta
     2941 aactttttta taacccctct tcctgacatg aactggtctc aaggagcaga attaataaat
     3001 aaagcaaaac atggagaagc cgtctcattt acagtgctcc ctccatggtt aactcttgat
     3061 ttaataaaaa aataaaactt cacattaata aataacaatg aatccgcctc cctacgtatt
     3121 cccgtaggga ggctgtcgtg tttatagtag cctggtaatg ctaaatgcaa acgaactgga
     3181 gattcctgct ccgctaagcg tattgcgcag ggtcacccta aatgtatcta caccaatata
     3241 agtaacatca caactaactg aatcaggcgc agatataggt gttatctgaa ccacataacc
     3301 attagtagct gctgcgagcg gatgagaaaa cgttacatca tacacaccag tagcatgcgc
     3361 gttaacggat gaaacacccg gcgcaatgtc tacactacca ttggcgttaa ttctccctga
     3421 gcgcgtaact gactctgcag aaaccgatgt ggccttactg gttatttcca taaaggtgca
     3481 accaatatcg atcaattcgc aattgttccc tgttatccag tatggtcgat ctgcacttgg
     3541 ctgataatcc cctgcgctct ggaacgttgt gccattgagt aagactataa cttttccccc
     3601 acctgagctt gttacttgta tattcgtgtg cgtgtagcgt tcactgctca cgcgatgaaa
     3661 atttccaccg tttataacta ccgttacagg tcgcgtccca gtattatcta tagcaaggtc
     3721 tgcccccccg gcattagcct cgaagtatgg gttgtttaac accaaagcac acgaaccgtt
     3781 aaggccattt atggctaatt gcgcaccacc tgtaccggaa tccccctgag taccatttcc
     3841 ttcacaagtc aggctgttga tagtagttcc tgccccccat ctatcaccaa gtatacccca
     3901 tcgtgtattc tggcttgcag ttaatcggtt aactgtaatg gcattaagct ctgacccttc
     3961 actggagtta agtgcgcgcc agccaatacg gttaccctgt aagtacacat tatctaactc
     4021 tgacgttaac accgagtctg tacgcaaccc ttcgccggta gtgtgcccat aagacacaat
     4081 gttctctaat ttagtgtatg ctttattggt tacgagaaga ccgtaagacg atcccgaacc
     4141 tttaagtgta atgtccccca gataatcata ggcgtgtaca cgttgggttg ccccgactgt
     4201 atcaccgagc atctttaagt tgaaatccga ggcatggcag ttaaatatcg tttctgccat
     4261 attttctccg gaataatgag cacggactcc tgtcgaacca ataaatttag cgtcatagtt
     4321 gccgtaatca agaacgagtg gtgacgcgat atcgtacacc ccttgcttta tttttaatgt
     4381 tccaccgaaa ggccacgatg taagcgggtc ttgatatatt tcatttttta ttgcaaatag
     4441 ctcctcttgg agatttttgc cgctattagt gccaatgtga gcagctcctt catctgattg
     4501 aagtaactgc tttaactgat caggatcgta cttcagcaca ttctgaaaat agaactgctg
     4561 cgaaccatac gcatcatata cagccattga atggccttgc tcagttacaa atttggcaat
     4621 ctgcccgttg tacacaggat atccggcagc attgataaca attggctgag aaacagggac
     4681 gtgagatccg tcttcattct ctacataaac ctgaatctgg ttttcaggat ttaccgggtc
     4741 agtgtcaatt tttccgatat aaattttgcc attggctacg gctttaaaag aacgcgccat
     4801 agtgaagagt tgcgaaggca tgctgaccac aacatttgcg gcgatatctg acatttcatt
     4861 gctccagacg aatgatatga tgcaatcatg atgtgattgc ataccgaaat ggcactattg
     4921 aatatttatc cagtaggtta cgatgccatt ccacccaact ggtgaggcat caaggatgta
     4981 cagcaaatac gacgaggcac agtttcactt gagacttccg catgaactcc acgcgaaaat
     5041 taaacagtgt gcgaagatga ataacagttc gctgaactca gagataattg cagcgattga
     5101 agaatcattg gctaaacaaa gctctgcatc agtttacatt gacgatgcag agcgtatggc
     5161 agaacaacaa tctgatatgg ttaagaaaat tgtctttgat acgctcaagg agctatataa
     5221 aaaagacagc aactaaccat ccgtggcttt ccgttacttc tggttatcat cactgttcat
     5281 ccagtacatg atgcctagcc ttgatatggt tttcttatct gattctggaa gtgagttgta
     5341 aaacgagcgc caagcagaag agcgtcttac agttgtatca acgatgcttt ctggtgcttg
     5401 ttttactgcc agccttgctg ctttctggaa ttctggtgag ctaattagct tttctgcaga
     5461 ttcaattgat tcagcgccgc ccatcgccct gattttgctg gcgacttttg ccccaatttc
     5521 tgacccagca acagcaccta ctgcactgaa cggtcctgac acagttgaac caaccgcagt
     5581 gccaatgcgt tgggcatggt tagcaacaaa ttcatttgcc gcagtgacgc gattaaaccg
     5641 tttgacgaac tcgtttagtc ttcctgtagt tatctcgtaa gattttgcat ctttgatagc
     5701 ctttgcgacc ttatatacat cgttcagacc tgacatagtc tcttttggta aatgtctggc
     5761 aagattgcgc atctgcccgt ttgctaacat gttttgatac cagtcagcaa accctgcagg
     5821 attaaaagca gcgccacgtt ttccgttcga aagcatatcc cgaagacctg ttcccagtag
     5881 ctcggttctt agcttcctgg acggcgtgtt ttgcatcaat tcacgaaatc ctttggcatc
     5941 gcctttcgac attgcctgta gtgctgtagt tgctttgcga gaaacatcac cgttcagcgt
     6001 tctgccagtt agattaatca tttgctcttc catgcttttt cgcatctgaa caagcctctg
     6061 agcaacttca aaatcacgta atgcatttgt ctctgacagc gccgcctttt gatcatcggc
     6121 gagcgaaccg taaagcctcg ataaagcagc gcgatcagca tctttatatg gtccagaatt
     6181 cttatgaagt gcatcaccaa caagtcgtcg ttgcttattg agatttgcat aagttaatgc
     6241 gccgttcttg cctggattaa ctgcccgaaa gactctcttt tcaatagggt caaggttttt
     6301 catcccgcca agatcttctg ccacctgttt gagcattgac cttgttgatg gcgcatcaat
     6361 ttttgcacct gcaggcatcg ccttttcaac attgcgataa agctggtcgc ttcgtttcat
     6421 cagcgcagac attcttccat tgaccgtatc aataaacttg tcgctcatac caagagcatc
     6481 ttttgcaccg gaaacgcgat caattatctc acccgcgccc tgtgctagct gcctgattgc
     6541 ttcattttcc tgcacctgta gagcagaacc cgcacgggac ttgattgctt gctcaactgc
     6601 cttgtattgc tcgttcccgg aaaagtgtga agggagtaat gaatcaacat taagcctttc
     6661 agcagactcc agaacttcag cctgaggatt gatatctagt tcatcaagcg atgaagcgag
     6721 atttggcctt ttttgtgctg ctactgtgcg aacgacttcc tctgggttct tcgtcgcagg
     6781 cgtcattggt acaccttgtg ctactcttgc ctctgcggca tgaaccgcag gatttgttgc
     6841 tgcagcggca tcaccggaga atggtgatgt ttctgttggc gcagtggtcg ctctctgtgc
     6901 agatgtttta ttgccactaa ttatatttga tgcaccacga acaccacggg taacaccatt
     6961 tatcaggcca tgcgtagcaa tgctaagtcc ggcattaata gcggcatttt cggcaaaatc
     7021 accccgttga ttcgccgcgt cagcgagaga accaatgacc atgtttccag ctacacctgc
     7081 tccaggaaca agataaccgc ctattgactc tccagcttgc gcgtaggggt ctgtcggtct
     7141 gtctactgga cgataaacat catccaatac ttttggcccc ccaagcccct gactgattgc
     7201 attaatcaga cttgcgccac cctgcaatac gtcaaatggt atgtttacca gaccacgacc
     7261 agcctgttct gcaatttacc ctgcactttg accaccagtg agccaatcgc cagcttgttg
     7321 catcaatgat ggttcttccc gtgttggtgc attattggcc tgattaactg tttgttgctg
     7381 aacaggtttc ggtgcgacag aacttacggg ttgaggtggc tgctgactgg ctgcctgctg
     7441 ctcaatctga gcaaatggat tatttgggtc tgactgcaca cctgatgccg atacttgttc
     7501 ggatgactgt acttccagtt gtgcaaacgg gttgttaggg tcttgctgag gatgtacctt
     7561 tgcagaggtg gcgcgctgtt cgactgttga gtctgtcacc gggtcaccag cccattgagc
     7621 aaagcgatca tcaacgtaac cgcggccttc aggtcctggc gtatattcac cacgctttgc
     7681 cttcataacg ttgccgggac cgtcgtgata agcctgaaga gcgtcacgcc agttaccaaa
     7741 ctgctggtat atctttgcca gatagcgcgc gccagcgtca gcctgatatt cggggttttg
     7801 catttgctca tcggtataac ccatatcacg ccatgtccca ggcatgacct gagtcaatcc
     7861 tacagccccg gcggagctta ctgcggcagg gttgtaagaa gactccttgg cacccagtgc
     7921 agtcatcaac ccttctggca caccgtaacg tgcgccagcc tgctctaaca aatcacggta
     7981 attagccatt tactgcccca aagatggaag atatccgtag cgattaatga agtcgattga
     8041 cagctcgggg tgctgcttca ggtaatctat agaagcctga ggcgcttcca ctcgcttgat
     8101 accgttttgc tgaacgtact taccaaccgc ctcattacgc ttctggttga gcgtgttcag
     8161 gatgacgcca gcgttgcgac gaaaggactc ctcgctctgc gagttctgca gcgaaccaac
     8221 agcctggtcg agctttttgc cctcggcatc agaaagtgcg cccatgcctc gcatggcctg
     8281 aaccgctgtc aggtatgctt gggatttaaa ggtatcaagt cgtgcctgag tgtctgcagc
     8341 ctgtgagcct ggaacgttgg ggattactcc acgtaagcct gtaatgctct taagtgaagg
     8401 agaactaacg atatcgttca gagttaacat gctggttgtg agggtgttga tgccgtcttt
     8461 gtagccatca tttagcgctt gctgcttctg ctgcaactgc tggttgttgg ctgctatgcg
     8521 gctctgtatt tcctggcgct tcaggtcgtt agtttctgct gatagcatcc ggtcaaggcg
     8581 cttattttcg ttgttaatgc ggtttgtttc tgcgtccaga ttaatgcgct gctgacctaa
     8641 attcgcctgg atatcttgcc cgcgcattgt gattgcctga ttccgagcgg cggtttgcga
     8701 atccaggtcc tgaccgcgca tggtaacctg gcgaccctgc attttatcct gtaggtcaaa
     8761 gtatttttcg ggtccgagac tgttcatccc caggtgatcg acaaattcgc cgaactgccg
     8821 cgggttctgt tggtacatct gagcgacgtc ctgaggatta acgccaacac gagctaactc
     8881 accggcgttg ttttgcagcc atgattgcat tgcttctgga gacgatgacg caaggcgtgc
     8941 gccagccgct aaggtgccga tagaattacg ctggtcttca tcaatgaatc ccatgccttt
     9001 acgaacggat tcaatctggt ctggatattg agtagccaac tgacgcaaag caccgcgatc
     9061 accagacgca taagcattag cgtacgcctg ctgaaattct ttctgccgct gagcctgctt
     9121 ttcctgctga aacacccccg caatacctga aaggccttgc aaagcagtca gcccaacatt
     9181 gttagcgcct gaacgctcaa tatcattgtt ctgcctgata agctgaagcg tattgccgat
     9241 gtcatttacg ttcggagcgt ttgagttgac gccgccga
//
LOCUS       DAJTYI010000137         9033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYI010000137 DAJTYI010000000
VERSION     DAJTYI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..770
                     /locus_tag="OO564_003335"
     CDS             <1..770
                     /locus_tag="OO564_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654342.1"
                     /translation="AWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSA
                     PVKPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKT
                     GWVAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSS
                     SPAQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVT
                     GWVVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1022..1939
                     /gene="oxyR"
                     /locus_tag="OO564_003336"
     CDS             1022..1939
                     /gene="oxyR"
                     /locus_tag="OO564_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6654343.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1922..3322)
                     /gene="sthA"
                     /locus_tag="OO564_003337"
     CDS             complement(1922..3322)
                     /gene="sthA"
                     /locus_tag="OO564_003337"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6654344.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3643..4293
                     /gene="fabR"
                     /locus_tag="OO564_003338"
     CDS             3643..4293
                     /gene="fabR"
                     /locus_tag="OO564_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6654345.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4293..4652
                     /locus_tag="OO564_003339"
     CDS             4293..4652
                     /locus_tag="OO564_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6654346.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4692..5792)
                     /gene="trmA"
                     /locus_tag="OO564_003340"
     CDS             complement(4692..5792)
                     /gene="trmA"
                     /locus_tag="OO564_003340"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6654347.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5906..6096
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6161..8005
                     /gene="btuB"
                     /locus_tag="OO564_003341"
     CDS             6161..8005
                     /gene="btuB"
                     /locus_tag="OO564_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6654348.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7950..8807
                     /gene="murI"
                     /locus_tag="OO564_003342"
     CDS             7950..8807
                     /gene="murI"
                     /locus_tag="OO564_003342"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6654349.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2069 a         2187 c         2435 g         2342 t
ORIGIN      
        1 tggcatgggg ctggcggacg atgttcgtta tcatcggcgt ggcagggatt ctggttggga
       61 tctgttggta cgcgtggtat cgcaaccggg cgcagtttgt cctgactgac gaagaacgga
      121 cgtatctctc cgcgccggtt aaaccgcgtc cacagctgca atttagcgag tggctggcgc
      181 tgtttaagca tcggacaacc tgggggatga ttttaggttt ctctggcgtc aattataccg
      241 gttggctcta cattgcgtgg ttacccggtt atttgcaagc cgaacaaggt ttcagtctgg
      301 cgaaaaccgg ttgggtagcg gcgattcctt tcctcgcggc ggcggtcggg atgtgggtta
      361 acggtattgt ggtcgatcgg ctggcgaaaa aaggctacga cctggcgaag acgcgtaaaa
      421 cggctattgt ctgcggtttg atgatgtcgg cattaggcac gttgctggtc gtgcaatctt
      481 cctcgccagc tcaggcggta gcgtttatct caatggcgct gttctgcgtg catttcgcgg
      541 gaacgtctgc atggggactg gtgcaggtga tggtgtcaga aacaaaagtg gcttccatcg
      601 ccgggattca aaactttggc agttttgttt ttgcttcctt tgctccgatc gtaaccggtt
      661 gggtagtgga taccacacac tcgtttaatc tggcgctggt tatcgcggcc tgtgtgacgt
      721 tcaccggggc gctgtgttac ttctttattg tcaaagatcg cattgagtaa tcgttactat
      781 caggctctct tgctgtgggc ctgtagaata aaaaaaagcg gatcctggag atccgcaaaa
      841 gttcacgttg gctttagtta ttcgagttga gaaactctcg aaacgggcag tgacttcaag
      901 ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc agtcagaatg cttgataggg
      961 ataatcgttc attgctattc tacctatcgc catgaactat cgtggcgatg gaggatggat
     1021 aatgaatatt cgtgatcttg agtacctggt ggcattggct gaacaccgcc attttcggcg
     1081 tgcggcagat tcctgccacg ttagccagcc gacgcttagc ggccaaattc gtaagctgga
     1141 agatgagctg ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt tcacccaggc
     1201 gggaatgctg ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga
     1261 gatggcaagc cagcagggtg agacgatgtc cggaccgctg catattggtt tgattcctac
     1321 ggttggacca tacctgctac cgcatattat cccgatgctg caccagacat ttccaaagct
     1381 ggaaatgtat ctgcatgaag cacagaccca ccagttactg gcgcaactgg acagcggcaa
     1441 actcgattgc gtgatcctcg cgctggtgaa agagagcgaa gcattcattg aagtgccgtt
     1501 gtttgatgag ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgaatg
     1561 cgtactgatg gccgatctgg caggggaaaa actgctgatg ctggaagatg gtcactgttt
     1621 gcgcgatcag gcaatgggtt tctgctttga agccggggcg gatgaagata cacacttccg
     1681 cgcgaccagc ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttatt
     1741 gccagcgctg gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccatgcat
     1801 taagccggaa ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag
     1861 ccgctatgag cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt
     1921 gttaaaacag gcggtttaaa ccgtttaacg cagctacccg ataggcttcc gccatcgtcg
     1981 ggtagttaaa ggtggtgttg acgaagtact caatagtgtt gccgccacct ttctgttcca
     2041 taatcgcctg accgatatga ataatttcgg cagcgcgctc gccaaagcag tgaataccca
     2101 gaatctcttt tgtttcccga tggaacaaaa ttttcagcgt gcccacgttc atgccgacga
     2161 tttgtgcacg tgccagatgt ttgaactggg cgcggcccac ttcatacggc actttcatcg
     2221 cggtcagctg ctgttcggtt ttgcccacag agctgatttc cgggatggtg taaatgccgg
     2281 tagggatatc ttcaatcaga tgtgcggtgg cttcgccttt taccagcgcc tgcgcggcaa
     2341 tgcgcccctg gtcataggcc gctgatgcca ggctcggata accaatcacg tcgcccaccg
     2401 cgtaaacgtg cggctgtgcg gtctgataca tgctgttgac cttcagctgt ccgcgactgt
     2461 cagtttccag cccaatgttc tgtaacgcca gcgaatcggt attaccggta cgaccgttgg
     2521 catagagcag gcagtcagct ttcagttttt tacccgactt cagatgcatg atcacaccgt
     2581 cgtcacagct ttcgatcttc tcgtactctt cgttgtgacg aatcactacg ccactgttcc
     2641 agaagtgata ggagagagaa tctgacatct cttgatcgag aaatgccagc aggcgatcgc
     2701 gggtgttgat cagatccact tttacatcca taccgcggaa gatcgacgca tattcacagc
     2761 cgatcactcc agcaccatag ataagtacat ggcgcggttc gtggtgcatg ctaagaattg
     2821 agtcgctgtc gtaaatgcgt ggatgggtga aatcaacatc tgttggatga tatggacgag
     2881 agccgcaggc aataacaaat ttttcagcgg ttagtgtttc aacgctgccg tccgggcaat
     2941 ccagcgccaa cgtatgctcg tcaacaaagc gagcgttccc ctgcaatatt ttacagtgat
     3001 tacgttcgta aaatccctga cgcatgcgcg tttgttgatt aatcacgtta tcggcatggt
     3061 taaggatatc ggcaaaagaa gagcggagca gtcgggaatg gtcgctgtat agtgggtttt
     3121 gattgaattc tataatgcgg ctgacggcgt gacggagagc tttcgacggg atggtgcccc
     3181 agtgggtgca accgccgcca acattttgat aacgctcgat aactgcgacg cgcgcacctt
     3241 gcttaaccag gcccattgca gcgccttcgc cgccggggcc ggaacctatt actatggcat
     3301 cgtaatcgta ggaatgtggc atggtagggc ttacctgttc ttatacataa aatcaacata
     3361 atggtaacat tctatcgcgg gtaagccaat tgatccccgt catttatctg gctatatcct
     3421 gagcggcctt tgctttgtct gtttcttact tttgccctga cgttttattg gatttttatc
     3481 gacgatactc tccgtttaag cggcaggttt ccgctgtacg taaaagaacc ggccagtaaa
     3541 tatgttttat tgcgttaccg ttcattcaca atactggagc aatccagtat gttcattctc
     3601 tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg cgtaagagcg
     3661 caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca attaagtgct
     3721 gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc
     3781 acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat ggttgatgag
     3841 agcggtttaa tgctacgcca actcatgcgc caggcacgtc agcgtatcgc caaaggcggg
     3901 agtgtgatcc gtacctcggt ctccacattt atggagttca tcggtaataa tcctaacgcc
     3961 ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc cgccgttgcg
     4021 cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga aaaccatatg
     4081 ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt cagtgcgggt
     4141 gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat tagaagagcg actggtactg
     4201 caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca agagaaaacc
     4261 gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa gatagaggta
     4321 cgctgctgct ggcgttggtt gctggcttat cgattaatgg tactttcgca gcgctgttta
     4381 gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg gtttactgcc
     4441 tgcatcaacg ttatcttaat cgcaccatgt cggtaggctt gccgggtctg gcagctgcct
     4501 gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat ccggatatcg
     4561 gctctaactt cttcccggca gtactctccg tcattatggt gttctggatt ggcgcgaaga
     4621 tgcgtaaccg taagcaggaa gttgctgagt aatcggtatc atgccggatg ctggcgcatc
     4681 cggcatgggt tttacttcgc ggtcagtaat acgccgcact ccatatggtg cgtgtagggg
     4741 aactgatcaa acagagccag acgttcgacc ttgtgcgtct ggcttaatgt ttccagattt
     4801 ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc ctgcaccatt
     4861 ttctcggttt cactgtccag accgctgcgc ggtgggtcga caaaaatggt ttcgaactga
     4921 tagctcttta agtcgatccc ttgcaggcgg ttaaactcgc gcacaccatt catcgcctga
     4981 gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt agcggcaatg
     5041 ttgtattgcg cggcagcgac tgacggtttg gcgatttcgg tggctaatac ccgatcaaaa
     5101 ttgcgcgcca gcgctaatga aaagttaccg ttgccgcagt acagctccag taaatcgcct
     5161 tttgagcctt tggttacgtc cagcgcccat tccagcattt gaatattcat cgccgcgttc
     5221 ggctgggtaa agctgttttc tacctgacga tagatcatct ctttccctgc gaccggcaga
     5281 cgctcgtcga tgtaatcttg atccagcgcg attttggttt tcgttgcccg accaatcaga
     5341 tgcacattca gattctgcgc gcgcagtgca tcgcgcagtg cttccgcttc ctgacgccac
     5401 tcatcatcca gctttttatg gtacagcagg gaaaccaccg cctgattact cagcgtggtg
     5461 aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac acccgcaatc
     5521 atcgccgtca tcaactggtt gatgagttca ctggcggcgg ggaagctatc cacgcggatg
     5581 cggcttttgg tttgttgatc gaaaatgatg tgatacaggt catcgccatc gtgccagatg
     5641 cggaactccg cgcgcatccg gtaatgactg accggcgagc gaaacacttc cggaaccagg
     5701 tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa ctgcgcttca
     5761 tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg
     5821 gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat ttgtgagcta
     5881 cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg tgagttaata
     5941 gggaatccag tgcgaatcta gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg
     6001 ttatgcggac actgccatcc ggtgggaagt catcatctct tagtatctta gatacccctc
     6061 caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg taatattgat
     6121 gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa aagcttcgtt
     6181 gctgacggcg tgttccgtca cggcattttc cgcttgggca caggatacca gcccggatac
     6241 tctcgtcgtt actgctatcc gttttgaaca gccgcgcagc actgtgcttg caccaaccac
     6301 cgttgtgacc cgtcaggata tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg
     6361 ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca ggtcagctct catctatttt
     6421 tattcgcggt acaaatgcca gtcatgtgct ggtgttaatt gatggcgtcc gcctgaatct
     6481 ggcgggggtg agtggttctg ccgaccttag ccagttccct attgcgcttg tccagcgtat
     6541 tgaatatatc cgtggaccgc gctccgctgt ttatggttcc gatgcaatag gcggggtggt
     6601 gaatatcatc actacgcgcg atgaacccgg aacggaaatt tcagcagggt ggggaagcaa
     6661 tagttatcag aactatgatg tctctacgca gcaacaactg ggggataaaa cacgggtaac
     6721 gctgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg gtaataccgg
     6781 aacgcaagcg cagacagata acgatggttt tttaagtaaa acgctttatg gggcgctgga
     6841 tcataactgt actgatgcct ggagcggctt tgttcgcggc tatggctatg ataaccgtac
     6901 caattatgac gcgtattatt ctcccggttc accgttgctc gatacccgta aactctatag
     6961 ccaaagttgg gacgccgggc tgcgctataa cggcgaactg attaaatcac aactcattac
     7021 cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt atgattcgtc
     7081 ggcgacgctc gatgagatga agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg
     7141 tcacggtagt attggtgcgg gtgtcgactg gcagaaacag actacgacgc cgggtacagg
     7201 ttatgttgag gatggatatg atcaacgtaa taccggcatc tatctgaccg ggctgcaaca
     7261 agtcggcgat tttacctttg aaggcgcagc acgcagcgac gataactcac agtttggtcg
     7321 tcatggaacc tggcaaacca gcgccggttg ggaattcatc gaaggttatc gcttcattgc
     7381 ttcctacggg acatcttata aggcaccaaa tctggggcaa ctgtatggct tctacggaaa
     7441 tccgaatctg gacccggaga aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc
     7501 tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga tcgattatga
     7561 tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg tcgaggcgac
     7621 agccaatttt gataccggac cactgacgca tactgtgagt tatgattatg tcgatgcgcg
     7681 caatgcgatt accgacacgc cgttgttacg ccgtgctaaa cagcaggtga aataccagct
     7741 cgactggcag ttgtatgact tcgactgggg tattacttat cagtatttag gcactcgcta
     7801 tgataaggat tactcatctt atccttatca aaccgttaaa atgggcggtg tgagcttgtg
     7861 ggatcttgcg gttgcgtatc cggtcacctc tcacctgaca gttcgtggta aaatagccaa
     7921 cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac gggaatacac
     7981 cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt gactccggcg
     8041 tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc cattacattt
     8101 atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc
     8161 gagtggtggc aattgtcacc gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg
     8221 cttgcaacac cgccagtacc gtttcacttc ctgcattacg cgaaaagttc gacttcccgg
     8281 ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat ggcattgtcg
     8341 gattactggc aacccgcgga acagttaaac gttcttatac tcatgagctg atcgcgcgtt
     8401 tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag ttggctgaag
     8461 cgaagctgca tggcgaagat gtttctctgg atgcactaaa acgtatccta cgcccgtggt
     8521 taagaatgaa agagccgcca gataccgttg tattgggttg cacccatttc cctctactac
     8581 aagaagaact gttacaagtg ctgccagagg gaacccggct ggtggattct ggcgcagcga
     8641 ttgctcgccg aacggcttgg ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg
     8701 cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg cccgttttac
     8761 agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt
     8821 tgcgcggtcg gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
     8881 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     8941 gaattccggc agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
     9001 cacacctcgc gccgctgaga aaaagcgaag cgg
//
LOCUS       DAJTYI010000138         9023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYI010000138 DAJTYI010000000
VERSION     DAJTYI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO564_003343"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO564_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6654350.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO564_003344"
     CDS             1828..3147
                     /locus_tag="OO564_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6654351.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="OO564_003345"
     CDS             3433..3474
                     /locus_tag="OO564_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654352.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="OO564_003346"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="OO564_003346"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6654353.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="OO564_003347"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="OO564_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="OO564_003348"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="OO564_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="OO564_003349"
     CDS             5803..6699
                     /locus_tag="OO564_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6654354.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="OO564_003350"
     CDS             6696..7682
                     /locus_tag="OO564_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6654355.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO564_003351"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO564_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>9023)
                     /gene="osmC"
                     /locus_tag="OO564_003352"
     CDS             complement(8657..>9023)
                     /gene="osmC"
                     /locus_tag="OO564_003352"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6654356.1"
                     /translation="STESGVLNQQPYGFNTRFEGEKGTNPEELIGAAHAACFSMALSL
                     MLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKSEVAVPGIDASTFDGIIQKAKAG
                     CPVSQVLKAEITLDYQLKS"
BASE COUNT         2189 a         2209 c         2281 g         2344 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gat
//
LOCUS       DAJTYI010000139         9000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYI010000139 DAJTYI010000000
VERSION     DAJTYI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(85..1461)
                     /gene="trkA"
                     /locus_tag="OO564_003353"
     CDS             complement(85..1461)
                     /gene="trkA"
                     /locus_tag="OO564_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6654357.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1483..2772)
                     /gene="rsmB"
                     /locus_tag="OO564_003354"
     CDS             complement(1483..2772)
                     /gene="rsmB"
                     /locus_tag="OO564_003354"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6654358.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2818..3765)
                     /gene="fmt"
                     /locus_tag="OO564_003355"
     CDS             complement(2818..3765)
                     /gene="fmt"
                     /locus_tag="OO564_003355"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6654359.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3780..4289)
                     /gene="def"
                     /locus_tag="OO564_003356"
     CDS             complement(3780..4289)
                     /gene="def"
                     /locus_tag="OO564_003356"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6654360.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4437..5561
                     /gene="dprA"
                     /locus_tag="OO564_003357"
     CDS             4437..5561
                     /gene="dprA"
                     /locus_tag="OO564_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6654361.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5533..6006
                     /locus_tag="OO564_003358"
     CDS             5533..6006
                     /locus_tag="OO564_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6654362.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6035..6577
                     /locus_tag="OO564_003359"
     CDS             6035..6577
                     /locus_tag="OO564_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6654363.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6582..7154
                     /gene="tsaC"
                     /locus_tag="OO564_003360"
     CDS             6582..7154
                     /gene="tsaC"
                     /locus_tag="OO564_003360"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6654364.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7159..7977
                     /gene="aroE"
                     /locus_tag="OO564_003361"
     CDS             7159..7977
                     /gene="aroE"
                     /locus_tag="OO564_003361"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6654365.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7974..8231
                     /locus_tag="OO564_003362"
     CDS             7974..8231
                     /locus_tag="OO564_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6654366.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8207..8761)
                     /locus_tag="OO564_003363"
     CDS             complement(8207..8761)
                     /locus_tag="OO564_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6654367.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2250 a         2188 c         2247 g         2315 t
ORIGIN      
        1 aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag ggaaataata
       61 aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga agagtctttc
      121 gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc cttgctcaat
      181 gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc caataatcgt
      241 tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc
      301 accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac
      361 aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat
      421 tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct ggatcaatac
      481 catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat tggcctcgtc
      541 atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg ccagcagttc
      601 ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg ccagttcggc
      661 agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca gacgacgcgc
      721 cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg gtttttccag
      781 tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa agaacacttc
      841 atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt ggcggaaaat
      901 tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc
      961 aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac cctcagcgaa
     1021 gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat cgataaccaa
     1081 ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata gcttatcggc
     1141 atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt tgaaaagtga
     1201 gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag caaccagcat
     1261 atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat gcccctgcac
     1321 gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg tatcgacaac
     1381 agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa cctggccggc
     1441 acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt tgattagctt
     1501 agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac ccggttgctc
     1561 tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg
     1621 caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc caccggattt
     1681 taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt ccgggatatc
     1741 gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg ttgctgaaca
     1801 aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg aagggtaacg
     1861 gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt cgtaaacgcg
     1921 agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg ccacctcaag
     1981 gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt caccgttttg
     2041 tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc
     2101 ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag catcagggta
     2161 atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc agctgtcgcg
     2221 ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat tggcttcggc
     2281 gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg
     2341 cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc gacgctggaa
     2401 ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg cgatagcgcc
     2461 ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca gttgatacaa
     2521 accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac gggccattaa
     2581 cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc acaactcttg
     2641 aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa
     2701 tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac gtaaattacg
     2761 ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag tggactatca
     2821 gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt cttgcgcgct
     2881 catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat caccagtcgc
     2941 aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt
     3001 atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca gccagctcat
     3061 tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa gtgaccagtc
     3121 aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac
     3181 ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc cttgtgggcc
     3241 aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga
     3301 gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa tggttacacc
     3361 agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg
     3421 cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca cagctttagg
     3481 cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg cgaccagttg
     3541 ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt cttcctcagc
     3601 cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg
     3661 ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc
     3721 aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag ttcttatcct
     3781 taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg ttgttgtttc
     3841 agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc atgctgaata
     3901 cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg gtcaagggcg
     3961 cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat cgacaggcaa
     4021 ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc
     4081 cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat atcaacctgg
     4141 gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata
     4201 cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc
     4261 ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa
     4321 agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac ttgattgaca
     4381 gcatcactga ccaatcgcaa agattgctaa ggcttgctta tgacagggag ataaggatgg
     4441 tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc gatgatatgg
     4501 tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa
     4561 cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag agtatcgaaa
     4621 gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt
     4681 atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt gaaggagaac
     4741 tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat tcatggtatg
     4801 gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg acaattacga
     4861 gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag gtaaatggcg
     4921 tcagcattgc tgtattgggg aatggactta atatcattca tccgcgtcgc catgcccgac
     4981 tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc ctcgatgttc
     5041 caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg
     5101 tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt tgtgcgcttg
     5161 agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc
     5221 ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa
     5281 acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag
     5341 atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa
     5401 cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac
     5461 tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga
     5521 ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacactgaa
     5581 gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggttttgag
     5641 cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta tcaggaaggg
     5701 ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta tacaccggaa
     5761 gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga gcagattcag
     5821 gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca
     5881 gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa catcccgggc
     5941 tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg
     6001 cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac tgttcacggt
     6061 gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca
     6121 cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc
     6181 ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg tatgtggcgc
     6241 aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga
     6301 atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg
     6361 tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga
     6421 tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag aatgccctga
     6481 gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc
     6541 cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag
     6601 gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc gcctatccaa
     6661 cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgagactgt
     6721 tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc
     6781 agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa accatttttt
     6841 cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt
     6901 tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt
     6961 tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac
     7021 cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg
     7081 gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt
     7141 ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc
     7201 aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg
     7261 cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt tcgtgctggt
     7321 ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag agcggatgag
     7381 cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt agaagatgga
     7441 cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga acgtctgtct
     7501 tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc tcgcggcgta
     7561 ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc
     7621 gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac
     7681 gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt
     7741 gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta tgacatgttc
     7801 tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat
     7861 gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct ctggcacggt
     7921 gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc cgcgtgaatc
     7981 aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt gtatgttttc
     8041 ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt ctggcgtatc
     8101 gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggacc
     8161 tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggctgggtct
     8221 ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg gaatagcgta
     8281 acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca
     8341 gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca atcatcacat
     8401 catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc
     8461 caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta
     8521 atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct
     8581 ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg
     8641 gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg ctatcgtcga
     8701 tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca
     8761 tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg gccttgtgca
     8821 agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa agggtgaaaa
     8881 aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg atccctataa
     8941 tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa caacgaagag
//
LOCUS       DAJTYI010000140         8994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYI010000140 DAJTYI010000000
VERSION     DAJTYI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..145)
                     /locus_tag="OO564_003364"
     CDS             complement(<1..145)
                     /locus_tag="OO564_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654368.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMD"
     gene            complement(190..1272)
                     /locus_tag="OO564_003365"
     CDS             complement(190..1272)
                     /locus_tag="OO564_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6654369.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1286..1945)
                     /gene="pgmB"
                     /locus_tag="OO564_003366"
     CDS             complement(1286..1945)
                     /gene="pgmB"
                     /locus_tag="OO564_003366"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6654370.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1942..4209)
                     /gene="ycjT"
                     /locus_tag="OO564_003367"
     CDS             complement(1942..4209)
                     /gene="ycjT"
                     /locus_tag="OO564_003367"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6654371.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGTQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4206..4812)
                     /locus_tag="OO564_003368"
                     /pseudo
     CDS             complement(4206..4812)
                     /locus_tag="OO564_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(4860..5606)
                     /locus_tag="OO564_003369"
                     /pseudo
     CDS             complement(4860..5606)
                     /locus_tag="OO564_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5637..6479)
                     /locus_tag="OO564_003370"
     CDS             complement(5637..6479)
                     /locus_tag="OO564_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6654372.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6466..7347)
                     /locus_tag="OO564_003371"
     CDS             complement(6466..7347)
                     /locus_tag="OO564_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6654373.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7368..8651)
                     /locus_tag="OO564_003372"
                     /pseudo
     CDS             complement(7368..8651)
                     /locus_tag="OO564_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8665..>8994)
                     /locus_tag="OO564_003373"
     CDS             complement(8665..>8994)
                     /locus_tag="OO564_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR6654374.1"
                     /translation="HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTI
                     DTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVNITDLHGRDLISEVDILGNEITLRP
                     WQVMWIK"
BASE COUNT         2139 a         2508 c         2214 g         2133 t
ORIGIN      
        1 catccatatc ttcgccataa ccctcgacgt tttctatttc gtacatcgca ccgatattaa
       61 agtgccagtc gttcttttcc tcggcctgtg cgcaggccat tcccgcacac atcaccagtg
      121 cggtacaggg taataacttt ttcattattg ttccttgtat tatcctaggg gaatgccttc
      181 cccctgtatt tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat
      241 atcaaaatga atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac
      301 cactaactcg tgccccccaa ccgtggtgta gagcataaat tccgcaccgg ttaattctgc
      361 cacgctaatt ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa tatcttccgg
      421 gcgtattccc atcacgatgg gcttatgcaa actttcctgt gttttcagaa ccgctaattt
      481 ctcttcgaga atggttaatt taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc
      541 gcgaataaaa ttcatcgctg gtgatccaat aaatccggca acaaacatat tcgcgggttg
      601 gttataaacg gttttcggcg cacctacttg ctgaacaatc ccgtctttca taatcacaat
      661 ccgcgtcgcc atggtcatcg cttcggtctg atcgtgggtc acgtagatca tggtggtgtt
      721 cagtttctga tgcagcttgc tgatctctgc acgcatttgt acgcgcagct tggcatcaag
      781 gttagagagc ggttcatcca ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag
      841 cgccacacgc tggcgttgcc cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg
      901 caggccgaga atttgcgccg cccagttcac ccgctcatca atcacctctt tggcgatttt
      961 ttgcatcttc aggccaaacg ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta
     1021 gttctggaac accatcgcga tattgcgtgc tttggctgga acgtcattca ttcgtttgcc
     1081 gtcgatcaac agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt
     1141 cgatttaccg cagcccgacg ggccgacaaa tacgatgaac tctttatcgg caatttccag
     1201 gttgaagtcc ttcaccacat gcacctggtt atcgtagatt ttttgaatat gttgtaacga
     1261 aagctgagcc atgttgattc ctttgctata cgttttgcca gaaggccgat aaccgcgacc
     1321 aggtcaggga gtccgttgag ggcaacagta attgcgcccc ggttaagccc gcaccgatcc
     1381 ccaccgagcg cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc
     1441 cgatacatgc ctgcggcggc acgcccagcc cggcacaggc agcgagaaag atttccggat
     1501 ccggtttcga gtttttaagt tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct
     1561 ccagcgccgc taaaatcgtc ggcgcattca gggagacgga agccagccct accggaatct
     1621 gctgtgcacg gagctctgcc agcaaattgc gaataccggg cagcacagca ttaaccgtca
     1681 gctcgcgcag tgagtggacg taaagcagat ttttgcgata cgccagttgc gcccgctcct
     1741 gcgggttaaa gtcgccctct ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc
     1801 ggctgatccc ttttagagat tcgttaaact gcgcatcaat actgatgcca atttcagcgg
     1861 caatctgctg ccacgcctgg aaatgcagat gcgcggtatc ggtgattaca ccatccagat
     1921 cgaaaattac cccttgcagt ttcattcatc ctcctgatgc gtggtagcgg tcccattgaa
     1981 gggcaaaata aaatcaccca aacagaaaac agattcttca gccacggata taagctgacc
     2041 gttcaaacgc agtgaaacgg gcgcagaagt tcgaatcgca atacgctgcg cgtcgagagt
     2101 gacctgtaat tcgcagccct gccagaacag agggaaagac aactgttgcc actgctcagg
     2161 taacgccgga tttagatgca attcaccgtc acgcacgctc accccggcaa aaccctgaat
     2221 cgcccccagc cagatagcgc cagttgcggc agcatggata ccatcatcac aactatgcgg
     2281 atcagcacca agatcgattt cagtcccctc gcgccagaac tgataacttt gggtcagcag
     2341 gccacagcgt gcagcaacaa tgccgtggat tgctttactt aatgacgagt cgtgaatagt
     2401 gcgcggttca taaaattgca gattggcaag acacgatgcc gatgagaact gctctggcag
     2461 catgtaattg agcatcacca catcagcttg tttgaggatc tgcatctcgt tcacttctgc
     2521 gcgtgaataa tccagcagaa tggtttgcgt ccccgccgcc gctttgtatt tcgccagatt
     2581 aatcgccggc ttagtcataa acgaatcatc ctgcggcaaa acaccgtcgg gctgaatttc
     2641 tggcagccgc agttctttaa ggaacatttc ggcacgatga ataaacgcgt cgtcactaca
     2701 gccaaactgg cgggcaatac tcagcgcctg ctggacgttg taatacacca tatagctggt
     2761 gaaggcgtta ttattgacat gttcggtata ttcgtctggc ccaataacat catgaatttc
     2821 cagacggtcg ttaaccctca ccgcgcggct aatccagaac tttgcagttt ccagaagtag
     2881 cgccatgcct tcatgcgcaa tgaaactttc atcccccgtg gtctgccagt attgaataac
     2941 cgcccaggcg atatcggcca ccagatgatg ttccgcctgc gccgaggcca ctttttgccg
     3001 cagcccggtg cgaatgttaa tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc
     3061 gctttcccac ggaaatagcg cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc
     3121 tggcaagttg tgccagcgat aacgcagtaa acttcgggca accgtcggat cgctaaacag
     3181 atggaacggc agcaaaaata cttctgtatc ccagaaaacg tggcctttgt agccttcgcc
     3241 ggttaagcct tttgccgcaa tactgctgcg ctcgccgtga gccggcgtca tgatgcgcag
     3301 atgataaagc gcataatcta acgcttgctg atcgtgcgca tcgccgccat ttaccgtgat
     3361 acgacgtttc tgccaccatt gacgccagtt ttctgttgat gctgcaagat gttggtcgta
     3421 actctgttgc gcgcacattt caagctggcg aagcgacacg ctgccccact cgtctaaagc
     3481 agcttgcctg tcatcccgcc agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc
     3541 tgcatgaagc tgcgcactgg tatgttgcag taaacggcgc tctttggcgg tatagcattg
     3601 ctgcacatca ccgctcacct ggcagcaaca actgatggcc acatcactgc gcccatcctg
     3661 ggtggtgtag atcccctgca tcaaatgctg accaaacacc cgcacctggg tttcgtcgag
     3721 atgctggcga ccgtggttgg tttgcgtggc gtcaatgcct gttgaaatca gcactgacgc
     3781 gtcggcgtcc agtggcataa tagtgatttc cagcgcaatg agcggcagtt ggtctgccga
     3841 aacaaagcga cggctggcga tggtgtaacc tgcgccgttg ctggtacgcc agacaacgct
     3901 acggcgtaat tctccactgg caaagtcaag ttcacgctgc caggcttcgc gggataacga
     3961 gaaaacctca ccatttatgg caatctccat ccccaagata tcaggcagat tcaccagttc
     4021 gttgatttca ccttttcccg cccgatgata cagccccgcc agatacatcc ctcgcgcctg
     4081 gcgcgtgtaa tcttcttcat ggctggcgcg aagcccaaga tagccgttcc cctgcgccat
     4141 cagcgatgca tacttgttca gggtatgctg gctgaaatgg ggttctgata acgttactgg
     4201 cctggtcata attccacacg cgtccctgtt tctgcggatt gatataacgc cgccaccagt
     4261 tgctggatga tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga
     4321 ttgataaagg cttccatgct gcgcaaatgg cggttgtcgt ctgccatttc ccgttgcatc
     4381 agcgtcatta attcaccgtt gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg
     4441 gctttatcac cacaaaagct gacgttcata atcaactgtt cgcggatgtt gagtgcaaat
     4501 gacgtttcca gccacagaat gccgccgtta tgaaattcaa tggtgccaaa cagcgaatct
     4561 tcgacgctgt aagttgccgg atcccactcg ccaaattgac cacagctctt ttgcgtgccg
     4621 atcttttgaa aactatgcgc attcacgctt ttcaccgccg gaaagcccag cacatacatc
     4681 gcagcatcca gcatatgaat gccgatgtcg atccagttct ttattggtaa agacacccca
     4741 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4801 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaacgatg gtgaaagtcg
     4861 taggccaggc cgcgcagcgc cgattgcagc gcgtcggcgt aaccgctggt ttcgataatc
     4921 acatcggccc cctgcttgcc ggtcagcgtt ttgatctctt tgcctacgtc agtgccaatg
     4981 gggttaaggc agaaatcagc accgtggcga cgggcaatat cacagcgatg ggcgatagga
     5041 tcgacgccaa ttaccaccga tgcgccagcg cgtttcgcca gttggatggc aatttgaccg
     5101 atcgcgccaa gccctaccac cacgacaaaa tcccctacgc gtacgttggc atcgcgcacg
     5161 ccgctcatgg caaactgcgc cgggtcgtaa cagacggcgt ttttccagga gctgccttgc
     5221 ggcattttgc gcagcttgta gttattcact gcgttaatga tgaccgtctc ggagagcggg
     5281 ccgtagccgc atacgctgtc gcccaccgcg tagtctgtaa cgtcgctgcc gcactcgata
     5341 atgtcgccaa ccaccatgtt gccaagctgg aatttgccaa actcaatgcc gcgcggcgca
     5401 tctgccggac gcggggtgaa catctgccac tcgccgttaa agtcttcatc gataaacggg
     5461 ctggcggcgc ggaagtcgac cacttccgtt ccgtgtttcg gtgcgccgaa acgcacgcgg
     5521 atcttcactt cattggctaa aatcgcccga tcttcatact caaccagcgc agcaacacgc
     5581 ggtgctgtgg ctactaactt tttcatgtaa aactccttgt aaagaaactg gccgctttag
     5641 cccttcacgc caccggcggt caaaccactc ttaataaaac gttccgaaag cgcatacata
     5701 atcaccaccg gcaatgcggt caccagcgag gccgccatca tccgtcccca gatgtaatca
     5761 ggcgtactga acagcgcgtt caggcccact ggtaaggtga aattgctggc gctggagagg
     5821 aaaatcgacg caaacagata gtcgttccac gtcaccataa agcagtagac gaacaccgat
     5881 atcagcccgg acatcgccag gggcacggtg atacggaaga tgatttgcag acggttgagg
     5941 ccatccatca tcgccgcttc ttcgatctca tcagggatgg tgtcgaagta acttttcagc
     6001 ataaacacgg cggtgggcag ggtttgcgtc accatggtaa tgatcagcgc catctcggtg
     6061 tcgtaaatac caagcgcggt gatgattttg aacagaggca ccaccagcaa aatgccagag
     6121 aacatataca ccgtgtaaaa gctggcgttg atggtcatcc ggcctttaaa gcgcaggcgg
     6181 gaaagcgcgt aagctccgag aatgccgaga aataccgcca ccacggagga aaccaccgac
     6241 accaccatgc tgttacggaa gtagtcgaca aacgggaaaa tcatcgggtt aaaaatgtcg
     6301 acgtaatgct ccagcgtcca ctgttgcggc agcagcgtag gatgcagcga tatcgcctct
     6361 ttcgcgcttt tgaacgaggt catcagcatc acaaaaaacg gaaacagcgt gatgatgaga
     6421 aacagcgcca gcccgcagta aaaaccgata cgactgagcg tgcgtttatt tgttgccatt
     6481 gaggttcacc cgtttcctgg tcagcagaat gacagcgaaa ataatgatga agagcaccac
     6541 cgagatcgcc gccgctttcc ctaagtcatt aaaggcaaag gcggttttgt agagatagac
     6601 accaagaata tcgaccttgg ttgtcagcag ataaacatca gcgaacatgt agaacatcca
     6661 gatggtgcgc agggtcacca ccgtcgccaa gaccggcata attgcgggca gcgtgacgat
     6721 gcgaaaccgc tgccaggcat tagcgccatc catctctgcc gcttcgtaca gcgatttgtc
     6781 gatggtctgc aaaatcgcca gaaacgagat aaaggcatac gggaagtagc gccagatggc
     6841 gagcagcacc accagcacaa agctactgcc cggattatca aaccacagcg gtgcctgctc
     6901 atagagatgc agaagatcga cgccgaggta gttcacaatg ccgtagccgt tgttgaacat
     6961 gtatttccag gcaaacacca gcgaaatgga cggcgttacg taggagagga tcaccagcga
     7021 acgcgcggtt ttgcgcaggc ggaactcgcg gttgaaaaac atcgccacgg ccagcccgag
     7081 cacggtgctg cccgccacca ccagcgccgt ataccagacc gtcatccaca gcgagtgcca
     7141 gaagccggga tcggagagaa tgcgcacata gttactcacc ccaacaaacg ttgactcgat
     7201 gttgggattg agcggcagac gtaaaaaact gatttcgata ttcgacacca tcggccacgc
     7261 caccagaccg cccagcagta ataagctggg cgcgagaagc agcagcgcaa acggcatatc
     7321 ggaacgacct gaaaaaagcc tgttcattgc ggattcctgt cctgggttta gtgctgttcg
     7381 atcagttcat ccagcttttt ctggctcgct tgcagcgtgc cagggagatc ggctttaccc
     7441 acggtgacgt tatgcaccat ggagctcacc acaccagaac ccgtcacatc gcccatgcgg
     7501 gtaaagtttt tatcccctac tgcgccaaag acctgaatat ttggcagttc accgattagc
     7561 tgattcggta gttcacccag cgccttaata acgtcgttgt ctttccaagt ggcggtagtg
     7621 acaaccgctt tattcaccgg cagcgcagca cctggcgaca tcatcaccca gtcggcaatg
     7681 ttgtccgcct gctccataaa ggtgacaaat ttttctgctg cttcggtctt ttgtccggcg
     7741 gtaatggtca gcgaggtcaa catgccgtag accgcagagt ttttctcggt tggcaccacg
     7801 aaaccgacgt ttttcgggtc gccttcttta atcacagccg gaaggatata ggtggagtaa
     7861 atcgccatcg gcgcggtgcc gttcataaag gcgtctttca cttccatgat gtcgttagaa
     7921 cccggcatgg tgttggcagc aaggtcgcgg taataggtca gcgcctgcat catctctggt
     7981 gtatcaaggg tgattttgcc ttcggcgtta aagacgttgg cctagttgga taacgcaaac
     8041 tgggagaagg attgttccgt cagcacgctt tctgccgtag gcagcgcaat accgtatttt
     8101 ttattcgccg ggtcattcag tttctgtgca acgtccagca gctgttgcca gtttttcggc
     8161 tcctccagcc ccgcttttgc cagcacatct ttgcgatacc agatgccgcc aatccaggcg
     8221 ctgacaggaa caccggtcca tgcgctacca tcttcggtac gcacaatacg cagtacgcca
     8281 tcgtaaaacg cgccttcacc aacgttgctg atgaccgtgg caaccgcttt gcgatcgata
     8341 agctgctctt tatccataac tttggcgtag tcatggctgg tttcgatcac ttccggcagc
     8401 gagccgctac gtgaaagggt gatgactttg gtgttataag cgtcttcttc cacgggcact
     8461 tgtttaacgg tgatacctgg gttttctttt tcaaaacggg caatcaattt gctgataact
     8521 gcctgacgct cctgctccac cgaagaatgc ataaattcga tggatacatg ggttttattt
     8581 tcttctttac agcctgaaat cagggcgcag gaaaccagtg ctgataacag cacgattttt
     8641 gatttaatca tgagatgttc ctttttattt aatccacata acctgccaag ggcgcagcgt
     8701 tatttcatta cccaatatat caacttcact aatcagatcc cgaccatgca gatcagtaat
     8761 atttacatgc tgaatatttt tactgacatt aaacaatcca gtcagacaat taccatcagc
     8821 gttacttctt tgaatacgca ttacggatga attaaccgta tcgatggtaa aattattatc
     8881 cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact catgatatac
     8941 cgcatgcctt aatgtagctt catcgttcag ttctcgtgtt atctcttcac tgtg
//
LOCUS       DAJTYI010000141         8970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYI010000141 DAJTYI010000000
VERSION     DAJTYI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(319..663)
                     /locus_tag="OO564_003374"
     CDS             complement(319..663)
                     /locus_tag="OO564_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6654375.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            795..2705
                     /locus_tag="OO564_003375"
     CDS             795..2705
                     /locus_tag="OO564_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6654376.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2763..3458
                     /gene="tsaB"
                     /locus_tag="OO564_003376"
     CDS             2763..3458
                     /gene="tsaB"
                     /locus_tag="OO564_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6654377.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3498..4079
                     /gene="yeaY"
                     /locus_tag="OO564_003377"
     CDS             3498..4079
                     /gene="yeaY"
                     /locus_tag="OO564_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6654378.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4284..5969
                     /gene="fadD"
                     /locus_tag="OO564_003378"
     CDS             4284..5969
                     /gene="fadD"
                     /locus_tag="OO564_003378"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6654379.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6051..7166
                     /gene="rnd"
                     /locus_tag="OO564_003379"
     CDS             6051..7166
                     /gene="rnd"
                     /locus_tag="OO564_003379"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6654380.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7220..8185)
                     /gene="yeaX"
                     /locus_tag="OO564_003380"
     CDS             complement(7220..8185)
                     /gene="yeaX"
                     /locus_tag="OO564_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6654381.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8241..8858)
                     /gene="yeaW"
                     /locus_tag="OO564_003381"
                     /pseudo
     CDS             complement(8241..8858)
                     /gene="yeaW"
                     /locus_tag="OO564_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         2150 a         2225 c         2420 g         2175 t
ORIGIN      
        1 ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta agatggagaa
       61 aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga aaaccagagt
      121 tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt tagttttatg
      181 gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac agtgatatct
      241 tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat cgtctgactt
      301 attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt tatacttcgg
      361 gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga caacccaggc
      421 atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa tggtggcatc
      481 cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct gtgccagcgt
      541 gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag tgtagtagag
      601 cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac gaacgatagt
      661 catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca caatgtgagg
      721 ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc ctgttcaaat
      781 caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg aaagcgatac
      841 caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc caggcgatag
      901 aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc tacgcttacc
      961 tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca aaagcgttgc
     1021 aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa tatacgggca
     1081 acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc gaacagcagg
     1141 cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg ctgcgctcct
     1201 ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg gaagattcac
     1261 aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac tgcccgatgt
     1321 ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat gtggtggtgg
     1381 taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt ggcgagttga
     1441 tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac attgccagcc
     1501 agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa gacatcacca
     1561 tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct gatcgtcttg
     1621 cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc ggtaacctgc
     1681 gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat gacaccttgg
     1741 aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg gatgcggcat
     1801 ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc aatcagccgg
     1861 gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc acgccgctca
     1921 gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg atcttcacct
     1981 cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt gggatagaac
     2041 aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg ttactctgtg
     2101 tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta gcggcaatgc
     2161 tgcgaccgat tatcgaagct aacaacggtc gttgttttat gctttgtacc tcgcacgcca
     2221 tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt ttgttgcagg
     2281 gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat gcgcttcttg
     2341 tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg tcattggtaa
     2401 ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg cgcatggaag
     2461 attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat gccgtcatta
     2521 ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc gtgctggtga
     2581 tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc agtctgccgc
     2641 ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata ccatcctcca
     2701 ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa gcacgaagac
     2761 ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc ctgtggaacg
     2821 acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa cgaatcttac
     2881 cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt aacgctctgg
     2941 cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc atcgcgcaag
     3001 ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg acgatggcgc
     3061 aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac gcgcgaatgg
     3121 gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac ggtgaagaaa
     3181 ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt tccggcgaat
     3241 gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag agcgggctgg
     3301 ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg attgcttgtc
     3361 agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt tatttgcgta
     3421 acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta tgccctgaga
     3481 aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata ctggcaggta
     3541 cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa ggcagcagtc
     3601 ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac gttggtcagg
     3661 aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa acccgcctgg
     3721 aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa ccttctcgcg
     3781 gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt agacaactgg
     3841 ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat acgccctata
     3901 aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag caggtgatta
     3961 tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc tatggctacg
     4021 gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta accgaataat
     4081 tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat tcttacggta
     4141 aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg
     4201 tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg atgacgacga
     4261 acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg
     4321 ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg
     4381 tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca
     4441 agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga
     4501 aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg
     4561 gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc
     4621 ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc
     4681 acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg
     4741 gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc
     4801 gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg
     4861 gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat
     4921 acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg
     4981 cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg
     5041 tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta
     5101 tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa
     5161 aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt
     5221 tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg
     5281 gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc
     5341 tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata
     5401 ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg
     5461 atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc
     5521 aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct
     5581 ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc
     5641 gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag attgaagatg
     5701 tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca
     5761 gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac
     5821 tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc
     5881 gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag
     5941 cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat
     6001 ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacta
     6061 cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg atagccctgg
     6121 atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg
     6181 atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag
     6241 cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag
     6301 tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg
     6361 ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg
     6421 ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg accgaacgtc
     6481 agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc aagcttatgg
     6541 tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa
     6601 tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgccaggc
     6661 agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg
     6721 cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg
     6781 cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa
     6841 tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag
     6901 atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt aaagcgttta
     6961 aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc
     7021 tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg aaaccgcaga
     7081 acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca
     7141 atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc
     7201 cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac
     7261 aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat
     7321 ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata
     7381 aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg
     7441 gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt
     7501 cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga cgcgcagctt
     7561 cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt
     7621 cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat
     7681 ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa ctttctggat
     7741 ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg
     7801 acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg gctgagggaa
     7861 ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct
     7921 gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca atctgataac
     7981 tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat
     8041 cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg
     8101 ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta
     8161 cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga
     8221 aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg
     8281 gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg cccctgacca
     8341 cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga
     8401 tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa ctcttcattg
     8461 gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg
     8521 accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa
     8581 ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg
     8641 aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg
     8701 gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca
     8761 atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg
     8821 acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt
     8881 acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg
     8941 ggcttgcaag cctgtagcgc aaattttagt
//
LOCUS       DAJTYI010000142         8966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYI010000142 DAJTYI010000000
VERSION     DAJTYI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            72..2958
                     /locus_tag="OO564_003382"
                     /pseudo
     CDS             72..2958
                     /locus_tag="OO564_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3068..3916)
                     /gene="alkA"
                     /locus_tag="OO564_003383"
     CDS             complement(3068..3916)
                     /gene="alkA"
                     /locus_tag="OO564_003383"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6654382.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4050..5402
                     /gene="yegD"
                     /locus_tag="OO564_003384"
     CDS             4050..5402
                     /gene="yegD"
                     /locus_tag="OO564_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6654383.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5414..7354)
                     /gene="yegI"
                     /locus_tag="OO564_003385"
                     /pseudo
     CDS             complement(5414..7354)
                     /gene="yegI"
                     /locus_tag="OO564_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7351..8112)
                     /locus_tag="OO564_003386"
     CDS             complement(7351..8112)
                     /locus_tag="OO564_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR6654384.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8109..8768)
                     /locus_tag="OO564_003387"
     CDS             complement(8109..8768)
                     /locus_tag="OO564_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6654385.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2188 a         2373 c         2347 g         2058 t
ORIGIN      
        1 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
       61 aagtaacggg gctggttgtc ctgctgacgc ccggagacga tcctctcagg gcatttttga
      121 tatgggtact gtcagaatcc atcggcgctc tggcactggt gccgctggga ttgttattta
      181 aaccacacta tctgctgcgc catcgcaacc cacggttgct ttttgagtcg ctgctcacgt
      241 tagccatcac actgacgtta agctggcttt cgatgctgta tctgccgtgg ccttttactt
      301 tcattattgt gctgttgatg tggagcgccg tgcgcctgcc acgaatggaa gcctttttga
      361 tcttccttac cacggtgatg atggtgtcgc tgatgatggc cgcggatccc tccctgcttg
      421 ctacgccgcg tacgtacctg atgagccata tgccgtggct accgtttttg ctgatcctgc
      481 tgcccgccaa catcatgacc atggtgatgt atgcctttcg tgcggaacgc aaacacattt
      541 ccgaaagcga aacccgcttt cggaacgcga tggaatattc cgctatcggt atggcgttag
      601 tgggcaccga gggacaatgg ctgcaatcca acaaagcact ctgccagttt ctcggttaca
      661 gtcaggaaga gctgtgcgga ctcacctttc agcaactgac ctggccggag gatctcaata
      721 aagatctcca acaggttgaa aagctgataa gcggtgaaat aaacacctat tcaatggaaa
      781 aacgctacta caaccgcaat ggcgatgttg tctgggcgtt gcttgccgtc tcactggtgc
      841 gccacacgga tggcacgccg ctctatttta tcgctcagat tgaagacatt aacgagctaa
      901 aacgcaccga acaggtgaat cagcaactga tggagcgcat cacgctggct aacgaagcgg
      961 gcgggattgg catctgggag tgggagctga agccgaatat ttttagctgg gataagcgga
     1021 tgttcgagct gtatgaaatt cctccgcata tcaaaccgaa ctggcaggtg tggtacgagt
     1081 gcgtgctgcc ggaagatcgc cagcatgccg aaaaagtgat tcgttgcaat cacgctcgcc
     1141 ctttaagctg gaatttcgca ttgccgtgaa agatggcatc cgccatatcc gcgccctcgc
     1201 caaccgggta ctgaataaag aaggcgaagt cgaacgcctg ctcggcatta atatggatat
     1261 gaccgaggtt aaacagctta acgaggcatt gtttcaggaa aaagagcgcc tgcacattac
     1321 gctggattcc atcggtgaag ccgtggtctg tattgatatg gcgatgaaaa ttacctttat
     1381 gaatccagcg gcggagaaga tgagcggctg gacgcaggaa gaagcattag gtgttccgct
     1441 cctgacggtg ttgcatatta cttttggcga caacggacca ttaatggaga acatttacag
     1501 tgccgacacc tcacgttccg cgattgaaca agatgtggtg ttgcactgcc ggaacggcgg
     1561 cagttacgac gtgcattaca gtattacgcc gttaagtact ctggacggca gcaatatagg
     1621 ctctgtactg gtgattcagg acgtcaccga atcacgcaaa atgctgcgcc agctgagcta
     1681 cagcgcctcc catgatgcac tgacgcatct cgccaatcgc gccaattttg agaagcaact
     1741 gcgtatcctg ctgcaaacgg taaacagtac acatcagcga catgccctgg tgtttatcga
     1801 tcttgatcgc tttaaagcgg tgaatgacag cgccgggcat gcggcgggtg acgctttact
     1861 gcgcgaactg gcgtcgttga tgctgagtat gctgcgctcc agcgacgtgc tggcgcgact
     1921 cggtggtgat gaatttggtc tactgttgcc agactgtaat atcgaaagtg cgcgttttat
     1981 cgctacacgc attatcagtg ccgtgaatga ttatcacttt atatgggaag gccgtgtgca
     2041 tcgggtaggt gccagtgccg ggattacctt gattgatgac aacaatcatc aggcggcaga
     2101 agtgatgtcg caggctgata tcgcctgtta tgcctccaaa aatggtggac ggggccgggt
     2161 gacggttacg aaccacagca agctgccgca catagcgaac gggcggcgat gtcgcttgat
     2221 gaacagtggc ggatgattaa agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg
     2281 ccgcggatcc cggaagcgcg taatttgtgg ctgatttcac ttaagctctg gagttgcgaa
     2341 ggcgagatta ttgatgaaca aaaatttcgt cgtagcttca gcgatccggc acttagccat
     2401 gctcttgacc gacgggtatt ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt
     2461 aaaggcataa gcatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg
     2521 aatgatctgc ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt
     2581 attccggctg aagcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga
     2641 tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg
     2701 aatatggcag attacctgct acttgatggt gagttatgcg ccaacgtgca gggtaatttg
     2761 atggatgaga tgctgattac gattattcag gggcacgctc agcgactcgg gatgaaaacc
     2821 atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat
     2881 ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagcagt
     2941 tatttcgcga ttaactgaga gagcgtttgc ccattgcctg atgcgatgct aacgcgtctt
     3001 atcaggccta caaatcgctc attccgcagg cgggataagg cgctcgcacc gcttccggcg
     3061 accaacgtca tgcttcgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat
     3121 aagaacgcca gggcttccag cgctcggcat aacggcggat ttgtgccggt gtcatcccca
     3181 gaaatcgctg tttaatcaga taatcatccg gcagaaaaac atctttcgcc tgccagccgc
     3241 gcaaggcgaa ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcgttttca
     3301 tcgcctgctc cacatcgccc ggtattgtca ttggtaaggt gccctccagc gccgcatttg
     3361 ccagatgaat cagcgcctct gcccgtttca acggcatacc taacgctttt aatgcctgcg
     3421 ggtcggctgc tgccagccgc tgaggcgtgg ggaagcagat atactccgga aaatcatcca
     3481 gccgttcgcc ataaagctgt gccactctgg cggtcaattt tgccgccatc gccacgctca
     3541 ccagttggcc taaaatcgcc cgcacgccct gctcaaaagc atcaacactg ccgggtaaac
     3601 gcaatccggg ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc tgcgggttac
     3661 attgcagatc aagcaggcgg ctcattttcg ccagacactc agcggcaacg ggttctaaac
     3721 ctgcacttaa atttatgtgc agagtatggc gggctatatc cggaatagca gtcaccacgc
     3781 cgcgatattc gcccactgcc agactacggg cataataact gtccgcgacc gtttccacac
     3841 cactcaccgc acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc
     3901 agttcaggat atacatcgca tctccttatt catccgcttt cagcataaac gttattcaga
     3961 cgttacgctt tgctttcata ttccggttgt cgcgacggca acatttcgct aaagtcacgc
     4021 cccttcttca ccggcatggg gattatttcg tgtttattgg ttttgattac ggtacagcaa
     4081 actgttcagt ggcggtcatg cgtgacggta aaccgcattt gctaaaaatg gaaaacgaca
     4141 gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt
     4201 accgccatca tgacgttccg gcagacgacg atgaaacgca ggcgttgtta cgacgggcga
     4261 ttcgttataa ccgcgaagaa gatatcgatg ttacagcgaa aagcgtgcag ttcggtcttt
     4321 cctcactggc acagtacatt gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat
     4381 cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct
     4441 gcgcaatgat gttgcacatt cgccagcagg cgcaggcgca gctgccagaa gcaattactc
     4501 aggcggtgat tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc
     4561 aggcgcaggg gattctggaa cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc
     4621 agtacgagcc ggtagcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg
     4681 tgctggtggt ggatatcggc ggtggtacga ctgactgttc attgctgctg atggggccgc
     4741 agtggcgttc gcgtctcgat cgtgaagcca gtctgctggg tcacagtggt tgtcgtattg
     4801 gcggtaacga tctggatatc gccctagcgt ttaaaaacct gatgccactg ctgggtatgg
     4861 gtggcgaaac cgaaaaaggc atcgccctgc cgatcctgcc gtggtggaat gcggttgcca
     4921 tcaacgacgt tcctgcacag agtgatttct acagtagtgc caacggtcgt ctgcttaacg
     4981 atctggtacg cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc
     5041 agcgtttaag ctatcgcctg gtgcgtagcg cagaagagtg caaaattgct ctttcaagcg
     5101 tagcggaaac ccgcgcctca ctgccgttta tcagcaatga actggctacg ctgattagcc
     5161 agcgagggct ggaaagcgcc ctcagccagc cactggcgcg gattctggaa caggtgcaac
     5221 tggccctgga taacgcccag gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc
     5281 gttctccgct gattaaaaaa gcgctggcag aacagttgcc gggcattccg attgcaggcg
     5341 gcgatgactt tggctccgtc accgccgggc tggcacgctg ggcggaagtg gtatttcgtt
     5401 aaaagaaaat ggctcagcag cggcttaaat cagcctgcgc ctgagccagt ttttctgccg
     5461 cctgacgtaa cggttccatc aacggcatgg tccgtgctgg agcatgaagg cggaatcgct
     5521 gcaactctgt cgcgccgaca gtcaacgtcg attccagccg atgtcgtttg gcggccattt
     5581 tcgccattac cgcctgtctg tctgctggcg ttaccgcttc gttcggcctg aacacaaaac
     5641 ggcgttcaca gctcgctttc cagtcgatga ccgcctgggt cagatgatca ccaaaccctt
     5701 taacttgctt aacgccacga cgggtaacac ctgctgctgt ttcaatacca aaagaccata
     5761 acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc aacatcaata aaaaatccct
     5821 ccagaaactt ctacttctgc cgttcccttg cagtgtcatg aagtgctgcc agagcgagtt
     5881 tctcttcttc cggtaacccg agaatttcgt ccttcatttt ttcgagcatc gtccgtttgg
     5941 cgataaaacc ttccaggccg cctacctgct ggatctggcg gactaaatgg tcatagtcca
     6001 ttttcgcacg attaaatgcc tcacggcgtt gctgaacttc tgctttgtac gctttgcttg
     6061 tcagactgcc aataatccag atagccgcca gcacaggaac caaaataata taacgcggtt
     6121 ctgcctgaag gccgcaaagc agcagcgata atgctgacag tgcgatctca agcagaatga
     6181 tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg
     6241 gtaatggcag ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagacttttg
     6301 ccagcacaaa atccccgccg gtggtaatga cctcttcgcc gagatcaata aaatagataa
     6361 cgccttgatt atccagcgta caccacaggc agtcggtcaa atgagcggga taaacatgca
     6421 ttgccgaaac gggacatttc tttaactgtt ggcgcagaga atcaagcgcc gctacccacg
     6481 ctttagccgt cggacgcccg gttgccacgc cactttccgt gaatgcctgc tgaaacatgg
     6541 cttcaacatc actcggtaac atcgatagcg gaatcgatcg tggcggcggt tttaaaccac
     6601 gtcgctgatt atctgacgcg taggcataac ggaaatgggt aatatccgtc tccagcgcat
     6661 tacccgcatc agagataagc ggcacaccag aataaggatg ccggccacca aacaagacgt
     6721 gaaaaatcag caacgcaagg ccaaaattac cgtgattttc tgtgcgttca aagccgacaa
     6781 atgacgacaa ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat
     6841 gcagtgtgcc attggcgtta atctgaaagg agtcgctatc aatcaacacc actttgctgt
     6901 cgcgacctac cataaaactg ttctggttta cgtcacccac gacatgcccg tgctcgtgaa
     6961 ccgtagcaaa agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat
     7021 gagggtaacg ttgacgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag
     7081 aaacttttgg catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct
     7141 gcggccaggc gacataattc aacaattgcg cgtcagctgt cgcagccata aaggcaagtt
     7201 tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact
     7261 cattgatatc ataaaccgcg ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc
     7321 actcgccagt agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt
     7381 tttatcatca tccgtacgct cgtttaccgc cgggctggac aaaaactgct ttaacaattc
     7441 aggtaataaa tcgagttgtt cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa
     7501 gaaaggcaca tgcggagaat tatccaacat atttaacgcc agtcgctgga taccatccgt
     7561 aaatgctgca actttatgca cacgctcagt gctggtgaaa gtttccagtc tggaaacggc
     7621 atcttcatcg gtaatgaagt gcgtcatatt ggcatattca cccaccatcg gcgtaagcgg
     7681 caattgcaga ccgtgaccaa aatccaccac cacgccgcca tcgccaatct gcataatcaa
     7741 ggtgccatta gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa
     7801 ttctttggct tccgcctcgg caaaaagtcg ctgacgaacg gtcagcacaa tatccgtggc
     7861 gaggatatca ttaagcccca attccccacc ctgcactttt tgcgacatat aagccatcgc
     7921 ttcattgacg gcgagcatcg cgccttcgcc cccctgcgaa acgctacctg cgccatcagc
     7981 aaggaacatc accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc
     8041 atcctggcaa ggcaagtccg cgctgatgtg cgacgttcca acagcggatg cataaaccag
     8101 acgccagctc acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc
     8161 gtggagcggg aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt
     8221 aatccttgca gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc
     8281 ccctgaacac cgatggaaaa gaaagcaaac cttttatctt cttccccccg aaacactttg
     8341 ttggctgcag cctgccactc atcggttggt gcaccatcgg taatcaggaa aatccacggg
     8401 cgataatacg aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg
     8461 gctttggtaa tggccgcacc cattggtgta tcgccctggg caaacaggat gggcggaaaa
     8521 aaattagcgg cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg
     8581 agttccactc tttttaaagc cagcgaatcg gcaagcagct catcgcgaaa ggtaaccaat
     8641 ccggtgttaa gttcattaat aggtctgcca ctcatcgagc cagaaacatc cagcagcaga
     8701 atgcaggggc aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt
     8761 tcgctcataa gaaatccttt cttagagaat tattgaggtc cggtatcata ccgactgcaa
     8821 taatgaaata agagcaaacg aagaaacttt cagaattagt cccggcggcg cagaaaattt
     8881 tgctattcgc ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa
     8941 tttcaggagt gagggttagg gagagg
//
LOCUS       DAJTYI010000143         8866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYI010000143 DAJTYI010000000
VERSION     DAJTYI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..299
                     /locus_tag="OO564_003388"
     CDS             <1..299
                     /locus_tag="OO564_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6654386.1"
                     /translation="PGGAAKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFG
                     AYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            317..955
                     /locus_tag="OO564_003389"
                     /pseudo
     CDS             317..955
                     /locus_tag="OO564_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1246..1974
                     /gene="mpaA"
                     /locus_tag="OO564_003390"
     CDS             1246..1974
                     /gene="mpaA"
                     /locus_tag="OO564_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6654387.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1949..2914)
                     /gene="ycjG"
                     /locus_tag="OO564_003391"
     CDS             complement(1949..2914)
                     /gene="ycjG"
                     /locus_tag="OO564_003391"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6654388.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3033..3539
                     /gene="tpx"
                     /locus_tag="OO564_003392"
     CDS             3033..3539
                     /gene="tpx"
                     /locus_tag="OO564_003392"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6654389.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3583..5124)
                     /gene="tyrR"
                     /locus_tag="OO564_003393"
     CDS             complement(3583..5124)
                     /gene="tyrR"
                     /locus_tag="OO564_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6654390.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5272..6333)
                     /locus_tag="OO564_003394"
     CDS             complement(5272..6333)
                     /locus_tag="OO564_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6654391.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6330..7727)
                     /locus_tag="OO564_003395"
     CDS             complement(6330..7727)
                     /locus_tag="OO564_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6654392.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7883..>8866
                     /locus_tag="OO564_003396"
     CDS             7883..>8866
                     /locus_tag="OO564_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6654393.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATH"
BASE COUNT         2221 a         2371 c         2137 g         2137 t
ORIGIN      
        1 aaccgggagg tgcggcaaaa atgttgttct cacatgcaca gaaaacgctg gcctgggatg
       61 ccttcgcatt tactgaagtg ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag
      121 tcggtgcctt cggtgcttat cgtgatggac tggaagtcta tggccgggcg atggtttcgc
      181 aggatcgtca gttagtttcg ttacccgatt tctcacatta tgaactatat gacaaaccgg
      241 aagcagttca ggaggcgctg gcaaaagtta ttcctttttt caacacccat ctgggataag
      301 tttcatgaga aatattatga aaaatgtact gattcttggt gctggcggtc agatagcccg
      361 ccatgtgatt aatcaactgg cagataaaca aactattaaa caaactttat ttgcccgtca
      421 gccagcaaaa atacacaaac cctatccaac taatagccag attattatgg gggatgtact
      481 gaatcatgca gcactgaagc aggccatgca gggacaggat attgtgtatg ccaaccttac
      541 gggggaagat ttagatattc aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt
      601 taaacgtctg atctttgttc tttccctcgg tatttatgat gaagttccag gaaaatttgt
      661 cgaatggaat aatgcagtta ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc
      721 aattgaggct tctggactgg aatatacaat tcttcgccct gcctagctga ctgatgaaga
      781 tattattgat tatgagctta cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg
      841 taagagtgtc gcagcattaa ttacagatat catcgataaa cctgagaaac acattggtga
      901 gaatataggc atcaatcaac ccgggactga tggcgataag ccattcttca tgtaaatcaa
      961 ttatatagat accatcgtac tgtactatgt gcagtacgat ttattaaact tattaaggtg
     1021 aactgatcaa tcagatgcga ttaacatgtg tagaaccagt aagaataaaa tacgccatgt
     1081 atataacatt acgacaattc taatatctgc acatcaatga aaatgcaaac aacttccggc
     1141 aaaaaagtga tacaagatcg atgacatagc gttgaagaaa aggatttgat aatccgcaaa
     1201 atttgcacaa cactgcctgt aatacttcct gaaccagaga cgatcatgac cgtaacccgc
     1261 ccacgcgccg aacgcggcgc atttccgccc ggaacagaac attacggacg ttcattattg
     1321 ggcgcgccgt taatctggtt tcctgcccct gcagccagcc gcgaaagtgg tttgattctg
     1381 gctggcactc acggtgatga aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact
     1441 ttgacacctt ctttgcgccg ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag
     1501 ctggggttac gggccaatgc taatggtgtg gatttaaaca gaaactttcc ggcggcgaac
     1561 tggaaggaag gtgaaacggt ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta
     1621 ttactgacag gtgacaaacc tggctccgaa cctgaaaccc aggcgctgtg ccagcttata
     1681 caccgcattc aacctgcctg ggtggtctca ttccacgatc cactggcttg tattgaagat
     1741 cccagacaca gcgaattagg cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc
     1801 agcgtcggtt atgaaacgcc tggctctttc ggcagttggt gtgcagatct caacttgcat
     1861 tgcatcaccg cagagtttcc gcccatctcc tccgacgaag ccagcgaaaa atatctcttt
     1921 gcgatggcaa acttgctgcg ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa
     1981 gcgccggttc cacatctacc gccagccagg tcggtccgtc aagatcggcg aaactgacct
     2041 gcggcaccag cggtaaagcg gcgctaatgg cacgagaggt acacaacatg cagcccagca
     2101 tcagactgaa accttgtgca cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac
     2161 cggttttatc gagcttaatg ttaaccatct catagcgccc tttcagcgcc ttcagattgc
     2221 tacgagtatg acaactttca tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg
     2281 ccgcatcgtc ctgcgcagga agcggttgtt caagcatcgc aacgcctaaa tccgccaata
     2341 gctggcaacg cgccgccaac ccttctgcac gccaggattc atttgcatca acgatcagcg
     2401 tggcatcggg cacagcggtg cgaattgcca ccatccgctc actgataaga tggttatcca
     2461 gcttcacttt cagtaatttc gcgcctgcct gccagagtgt tgatgcacta ttggccatct
     2521 gatcaggcgt accgatgaca accgtctgtg cagtgataac tgtctcggga agcgttatgc
     2581 cgatcaaatc agccagcgat tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat
     2641 ccagcgcatt acgtgctgcg ccggcaggga gaattttttg caactcctcc cgtgtcagcc
     2701 ctttctctaa ttgcggcacg acgctcataa tttgcgccat taccgaggca tcactttctc
     2761 cataacgcgg atacggcgtg cattcgccgg tgcctttaat accctcttct tccagttcaa
     2821 ccaccaccac gcgcgcttca ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg
     2881 gccaggcttc ctcaaatacc ttaacgattc tcatctttac tccttgttgt cgcggtggtc
     2941 tgcggattaa gcgcaaatag cgtttgctgt gttattgaca gttagcataa actaggtgtg
     3001 acgttaacta tatgtaaaca ggaaagataa tcatgtcaca aaccgttcat ttccagggca
     3061 acccggttac agtcgccaat tccatcccgc aggcgggtag caaagcgcag acttttactc
     3121 tcgtggcaaa agatctgtct gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc
     3181 tgaacatttt cccgagtatt gataccggtg tttgcgccgc atcagtacgt aagtttaacc
     3241 aactggcaac tgagatcgac aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg
     3301 cccagtctcg tttctgcggc gcagaaggtc tgaacaacgt tatcaccctc tccactttcc
     3361 gtaacgctga atttctgcaa gcctacggtg tggcaattgc tgatggccta ctgaaaggtc
     3421 tggcagcgcg tgccgttgtg gttattgacg aaaatgacaa tgtgattttc agccagctgg
     3481 tggatgaaat caccaccgag ccggattacg aagcagctct ggctgtactg aaagcataag
     3541 ttaatatgcc tgatggtgtt gcaccatcag gcatattcgc gcttactctt cgttcttctt
     3601 ctgactcaga ccatattccc gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg
     3661 ttttgccagt ttgcgcgtgc tgggataatt gcgataaagc tgggttaata ccgagcgttc
     3721 aaaacggctg gtgatttcgt ccagcgaacc ttccatcgca tcttcgccca cggctaccgt
     3781 tgctgcgtca taatccggca acaaaatatc ctgtggacgc agctcataac cgtccagttg
     3841 tgtcagcgcg cgatagatag cgttctttaa ctgccgcaca tttcccggcc acgcataacg
     3901 tgtaagtaca gtattcaggt cagcggccag tttcggacgc ggcacgccct gctcgtcggc
     3961 aaagcgggcg acgaacagct cagttaacgg cataatgtcc tgcggacagt cacgtagcgg
     4021 cggcagattg agcgtcaaca cgttcagacg ataatagaga tcttcacgga acacgccttt
     4081 ttgcaccagt tcgaccagat tcttctgcgt agcgcaaatc acccgcacat cgacatgcac
     4141 ctcatggtct tcgccaaccc gacggaaagt gccatcatta agaaaacgca gtaatttcgc
     4201 ctgcatccgt ggtgacattt cccctatttc atccaacagc accgaaccac cgttcgcctg
     4261 ctcaaagaac cctttcttcc cttccggagc atgaccaaac agttcactct cgaccgcatc
     4321 ttctggtata gacgcgcaat tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg
     4381 atggcaggcg taggcaaaaa gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg
     4441 cgcgcttagc atcgccagtt tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac
     4501 ggcgacaatt tggctgaagg cgctgacgtc ctgggcggcg acattttgca actggcggcc
     4561 catacgaatc gttgatcgca acatcaccac cgcaccggtc aggacgtgtt gatcattttc
     4621 atcctgaaga taaacaggcg taatctccat caggaaattc tgcccattaa taacgacatg
     4681 ctcgttatgc gaatcttgcg gttcgctttc cagccaacgt aaaaaattaa agccgttaat
     4741 caattgtgcg gcggtatggt tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct
     4801 cgccgggttc gccatatcca ctttgctttt catatcgaca gagagcacag gttcaggcaa
     4861 cgcctccagt aacgcgctca acgccagatg ctcacgttcg gaaggcatcc acgggacagt
     4921 acgcacatcg gtaacacctg caatacggcg tatttcggcc atcaaactgc tgaaactctc
     4981 aaactccagt tcagcaaaat tgaggtagat tcgcccaatg ggatcaatct caataccgcg
     5041 taaatcaatg cctcttagca cgagtagatc gagtaattcg cgggtcagac cgagtcggtc
     5101 ttcacaaaag acttccagac gcatgggaac cttcacctga aaaaagaaca attaatatga
     5161 tgatatgaca ctatttggat agcaggaagg tttctgtcaa caatcattga cacaaagacg
     5221 gaaaaacggg cacttcggcg taaagatatc cggtcagcac ccgatattgc attatttttc
     5281 gctgggcgtt ttgccttttt gcagagtttc tttcacctga ccgataagct gacgacggaa
     5341 atccccaagg cgaggtttgt catcgtcaat ccacggcagc gggcggcaaa gctccatagc
     5401 tttaatcccg agtcgtgccg tcagaagtcc agctccaatt ccctgagctg cgcgggtaga
     5461 caaacgagca gcgagatctt gcgacatcca gtccatcccc acttcgcgca ccagttcgct
     5521 ggctccggca aaagcgatat tcagcaatac cagcttaaac agacgcaaac ggctgtaata
     5581 ccccagttca atgccataca gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc
     5641 gataaacgcc atatcgacca acgccagcgg gctgaccgca atcatcaacg ttgattccgc
     5701 cgccgaacgg ctgatttcgc gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc
     5761 atacaggctg accacttcac gatcgttctg cgtttcatgg attgaggcat accagcgttg
     5821 cagcgccgga tgcgactgat caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg
     5881 acctttgccc gtgccgtggc tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg
     5941 ctgtcgcaag cgccataagc gccgccactc tgttaccaca gaacctacgc cagcgccgat
     6001 aatcaatgcc ccagcggcac atccacccag cgctacccag tcctgggttt gccaggcatt
     6061 cattgtccac tgtataccct gcccgacaac gcttgcgcca aacagagcca gcccgcccat
     6121 caccattttg cgccacaggc tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac
     6181 ttgcccctct tcttcctgcg cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt
     6241 ttcgtcaaag gtctgctgcg ccctgaattt tggattctgt tcgacctcca gcggaccgtc
     6301 gaaatcaata cgtggtttta acggttcggt catcgcaatt tatctcctat taaaaattcc
     6361 agcgcggcat caagacgaat atgcggcagc ggtttgtcga catccatcac ctgcgggcga
     6421 aacgcttcaa actggaaccc ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga
     6481 acttcaccgg ggtaaacggt gagaggcgca ccatcgctga gccggttacc gcgcaatgcc
     6541 ggaattttct cgccgttgac gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc
     6601 cccaggcaat ccatactaat cctttcaaac gccgcatttt gccaggcatc ctgaatcagt
     6661 tgttgcagta atgaaaccat attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc
     6721 gcggcaaaca gcagtttgtc gataaccggc gagaataagc ggcgaaacag cgtgcgctgc
     6781 ccgtagtgga aactttgcat cagctgcgtc agtgccagac gcatatcatt aaatgcctgt
     6841 ggcccactgt tgagaggttg caggcaatcc accagcacaa tctggcggtc aaagcgcaga
     6901 aaatgattct tatagaaccc cttcaccact ttctcgcagt aataattaaa ccgctcgcgc
     6961 agcattccgg cattggtgtg cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg
     7021 acatccggcc acgggaagaa ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca
     7081 aagcgcccag gctgaataaa gtgcagcccc tgctgtttac agtggtggag ataatcggtc
     7141 cacacggcgg caatgtctgc cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct
     7201 tcgcacatca ttcgccattt cgccgaccat tcgccgcgct gaccattgag taagcccgtc
     7261 atctggcgcg accagcttaa gtaatcctgc gccagcatcg gcaggtcgag caaccattcg
     7321 ccgggataat ccacaatttc cagatacagc gtggaggtat ccttaaagtg gcgcagcagc
     7381 gaatcgttcg atttaaaacg taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc
     7441 ggccaggcgg gaggatcacc atataactgc gccagtcctt catcataggt gaagcgcgga
     7501 atgccaaagt cacgctgggg aatgcgtttt acgcccagca agcgctcttc acgcaccgcg
     7561 cttaacagcg gcaaacgtgc tccggcatga atattgagca actgattaac catcgcggtg
     7621 ataaacgctg ttttgccgct gcggctaagt ccggttacag cgaggcgcag atgtctgtcg
     7681 acaccccgat tcaccagcgc attgagttca tttttaagtc gcttcattgc tgtcctgtgc
     7741 tgctctgaag accagaaaaa aaatcattcg taaggatatg aatgtggggg ccaggccatt
     7801 gattatcaac tgcccgtgtt gtcatcgacg ggcgaactcc ataagatgtg cagcattcac
     7861 gttcggacgg agttcctgac ctatgtcccc tactatttat gatattgcca gggttgcagg
     7921 cgtatcaaaa tccaccgtat cacgcgtgct gaataagcaa actaatatct ccccggaagc
     7981 gcgcgaaaaa gtgttacggg ccattgaaga attacagtat cagccaaaca agctggcccg
     8041 cgcgctgacc tcttcgggtt ttgatgccat tatggtgatt tctacccgtt cgaccaaaac
     8101 tacggcgggt aatccgtttt tctctgaagt tttgcatgcc atcaccgcca aagcggaaga
     8161 agaaggtttc gacgtgatat tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg
     8221 cgaaagcaaa attaaacaga aaatgattaa aggcattatt atgctcagtt cgccagcgga
     8281 tgagtcattt tttgcccaac tcgataaata cgatattccg gtggtggtaa ttggcaaagt
     8341 tgaaggtcaa tatgcccatg tttattctgt cgataccgat aattttggtg acagcattgc
     8401 gttgaccgat gcattaattg aaagtgggca tcaaaatatt gcctgcctgc atgctccgct
     8461 tgatgtccat gtttcggttg atcgcgttaa tggctataag aaaagtctgt ccgcgcataa
     8521 tattgcagtg cgtgataaat ggattgttga cggcggttat acccatgaaa cagcactgca
     8581 agcagcacgg caattattaa gtcagtcacc gttgcctgag gcagtgtttg ccactgacag
     8641 cctgaaatta atgagcattt atcgtgcggc ggcagagaaa aatattgcta ttccgcagca
     8701 gttagcggtg gtgggttata gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg
     8761 cggcatcgat gttccgacgc aggagttagg tcaacaaagc tgcgagttat tattccgctt
     8821 aatttcggga aaaccgtcac cacagaatat taccgttgcc acgcat
//
LOCUS       DAJTYI010000144         8807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYI010000144 DAJTYI010000000
VERSION     DAJTYI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..936
                     /locus_tag="OO564_003397"
                     /pseudo
     CDS             1..936
                     /locus_tag="OO564_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1002..1250
                     /locus_tag="OO564_003398"
     CDS             1002..1250
                     /locus_tag="OO564_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6654394.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1315..1683
                     /locus_tag="OO564_003399"
     CDS             1315..1683
                     /locus_tag="OO564_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6654395.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            1777..2430
                     /gene="nfsB"
                     /locus_tag="OO564_003400"
     CDS             1777..2430
                     /gene="nfsB"
                     /locus_tag="OO564_003400"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6654396.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2538..3785
                     /gene="ybdG"
                     /locus_tag="OO564_003401"
     CDS             2538..3785
                     /gene="ybdG"
                     /locus_tag="OO564_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6654397.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(3853..5229)
                     /gene="pheP"
                     /locus_tag="OO564_003402"
     CDS             complement(3853..5229)
                     /gene="pheP"
                     /locus_tag="OO564_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6654398.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5330..8465)
                     /gene="cusA"
                     /locus_tag="OO564_003403"
                     /pseudo
     CDS             complement(5330..8465)
                     /gene="cusA"
                     /locus_tag="OO564_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(8477..>8807)
                     /locus_tag="OO564_003404"
     CDS             complement(8477..>8807)
                     /locus_tag="OO564_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6654399.1"
                     /translation="KTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALIDTGNEQRVI
                     TVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANISGALERMRS
                     ESATHAH"
BASE COUNT         2133 a         2453 c         2165 g         2056 t
ORIGIN      
        1 gcgacggatg tttgccgtga tatcaaccag gctgccgggc agttttcagc gatgcagaaa
       61 gtcgtattgc aggcagccgc agaccatcat ctggaaattt gcggcggtgg cacgcccccg
      121 tttcagaaat ggcagcgtca ggaggtatgc gacaacgaac gctatcaacg aacgctggaa
      181 aactttggct atctcatcca gcaggcgacc gtttttggtc agcatgtcca tgttggctgt
      241 gccagtggcg atgacgccat ttatttgctg cacggcttgt cacggtttgt gccgcatttt
      301 attgcccttt ccgccgcgtc gccatatatg cagggaacgg atacgcgttt tgccccctca
      361 cgaccgaata ttttttccgc ctttcctgat aatggcccga tgccgtgggt cagtaactag
      421 caacaatttg aagccctgtt tcgctgtctg agttacacca cgatgatcga cagcattaaa
      481 gatctgcact gggatattcg ccccagtcct cattttggca cggtggaggt tcgggtgatg
      541 gataccccgt taacccttag ccacgcggtg aatatggcgg gattaattca ggccaccgcc
      601 cactggttac tgacggaacg cccgttcaaa catcaggaga aagattacct gctgtataaa
      661 ttcaaccgtt tccaggcctg tcgctatggt ctggaaggcg tcattaccga tccgcacact
      721 ggcgctcgtc gaccgctaac ggaagatacc ttgcgattgc tggaaaaaat cgccccttca
      781 gcacataaaa ttggcgcatc gagcgcaatt gaagcactgc atcgccaggt cgtcagcggt
      841 ctgaatgaag cgcagctgat gcgcgatttc gtcgccgatg gcggctcgct gattgggctg
      901 gtgaaaaagc attgtgagat ctgggccggt gactaagccg ggaattgcct tacagaagaa
      961 tatccctgac aatggtgttt taacttatat ttaacaatgt tatgaaacac cctttagaaa
     1021 ccttgaccac cgcagcaggc attttgctga tggctttcct ctcttgcctg ctgctgcccg
     1081 cccccgcact ggggctgacg ctggcacaaa aactggtgac catgttccat ctgatggatc
     1141 ttagccagct ttacacttta ttgttttgtc tgtggttttt agtgctgagc gctattgagt
     1201 attttgttct gcgctttatc tggcgacgct ggttctcgct ggcggattaa acgtcgatta
     1261 tcgccgaact tcgcggcata ctttgcttat tctccttcgc ctaaaggaac gtttatggat
     1321 aagcaatcac tgcacgaaac ggcgaaacgc ctagctcttg agttaccctt tgtcgagctt
     1381 tgctggcctt ttggcccgga gttcgatgtt tttaaaattg gcggcaagat ttttatgctg
     1441 tcgtcggagc tacgcggcgt cccctttatc aatctgaagt ccgatccaca aaaatccctg
     1501 ttaaatcagc aaatataccc aagcattaag ccaaggtatc acatgaataa aaagcactgg
     1561 atttcggtgt attccggcga ggaaatctcc gaagcgttac ttcgcgatct gatcaacgat
     1621 tcgtggaatc tggtggttga tggtctggct aaacgcgatc aaaaaagagt gcgtccaggc
     1681 taaagcggga atctatagcg catttttctc gcttaccatt tctcgttgaa ccttgtaatc
     1741 tgctggcacg caaaattact tacacatgga gtctttatgg atatcatttc tgtcgcctta
     1801 aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac ttaccccgga acaggccgag
     1861 cagatcaaaa cgctactgca atacagccca tccagcacca actcccagcc gtggcatttt
     1921 attgttgcca gcacggaaga aggtaaagcg cgtgttgcca aatccgctgc cggtaattat
     1981 gtgttcaaca aacgtaaaat acttgatgcc tcgcacgtcg tggtgttctg tgcaaaaacc
     2041 gcgatggacg atgcctggct gaagctggtt gttgaccagg aagatgccga tggccgcttt
     2101 gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca agttcttcgc cgatatgcac
     2161 cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac aggtttatct caacgtcggt
     2221 aacttcctgc tcggcgtggc ggcgctgggt ctggacgcgg tgcccatcga aggttttgac
     2281 gccgcaatcc tcgatgcaga atttggtctg aaagagaaag gctacaccag tctggtggtt
     2341 gttccggtag gtcatcacag cgttgaagat tttaacgcta cgctgccgaa atctcgtctg
     2401 ccgcaaaaca ccaccttaac cgaagtgtaa ttctctcttg ccgggcatct gcccggctat
     2461 ttcccctcag attctcctga tttgcataac cctgtttcag ccgtcatcat aggctgctgt
     2521 tgtataaagg agacgttatg caggatttaa tatcccaggt tgaagattta gcgggtattg
     2581 agatcgatca caccacctcg atggtgatga ttttcggtat tatttttctg accgccgtcg
     2641 tggtgcatat tattttgcat tgggtggtac tgcggacctt cgaaaaacgt gccatcgcca
     2701 gttcacggct ttggttgcaa atcattaccc agaataaact cttccaccgt ttagctttta
     2761 ccctgcaggg aattatcgtc aatattcagg cggtattctg gctgcaaaaa ggcaccgaag
     2821 cggcagatat tctgactacc tgcgcgcagt tgtggatcat gatgtatgcg ctgctttcag
     2881 tcttctcgtt gctggatgtt attttgaatc tggcgcagaa attcccggcg gcatctcagt
     2941 taccgctgaa agggatattt caggggatta aactgatcgg cgcaatcctg gtcggcattt
     3001 tgatgatctc gctgctgatt ggtcagtcgc cagcgattct gatcagcggt cttggtgcaa
     3061 tggctgccgt gctgatgttg gtatttaaag atccgattct tggtctggtg gcaggtattc
     3121 agctttccgc gaacgatatg ctgaaactgg gcgactggct ggaaatgccg aaatacggcg
     3181 cggatggcgc ggtgatcgat attgggttaa ccaccgtcaa agtgcgtaac tgggacaata
     3241 ccattaccac tattcccacc tggtctctgg tttctgactc ctttaaaaac tggagcggga
     3301 tgtcagcatc tggcgggcga cgtattaagc gcagtatcag tattgatgtc accagtattc
     3361 gttttcttga tgaagacgaa atgcaacgtc tgaataaagc gcatttgtta aagccttatt
     3421 taaccagccg ccatcaggaa attaatgagt ggaatcgcca gcaaggttct acggagtcgg
     3481 tattaaacct gcgccgaatg accaatattg gaacctttcg tgcctatctg aacgaatatc
     3541 tgcgtaacca tccgcggatt cgtaaagata tgaccttaat ggtacgccaa ctggctccag
     3601 gtgataacgg tttaccgctc gagatctacg cgtttactaa caccgtggtg tggctggaat
     3661 atgaaagcat tcaggctgat atattcgatc acatatttgc gattgtcgaa gagtttggtc
     3721 tgcgacttca tcagtcgcca accggcaatg atattcgctc tctggcgggt gcatttaagc
     3781 agtaattaaa aaaaccgctc tcatcgaatg gatgagagcg gacatcgttt tgtcggatgt
     3841 agcgtgaatg ccttatttcc gacgcagcgt tttaaatgcc acaaacagga atacaatcca
     3901 caccggcagc aggatcgctg acaagcgcat atcatccatc gtgcacatca gcagcaaaat
     3961 catgccgagg aaggcgatgc aaagatagtt gccgaacgga tagagcagcg ccttaaactg
     4021 tgtttcacgc ccctgacgtc gcatcgccgc gcgaaaacgc agatgcgcca ggcagatcat
     4081 aatccagttc aacagcaacg ttgctaccac cagcgtcatc agcagaccaa acgctttttg
     4141 cggcagcaga tagttgatta acaccaccag cgaagtgatc gctccggaaa gcatcagcga
     4201 gttaatcggc acaccgcgac ggctgacgcg agtcaaaaac ttcggcgcat taccctgcac
     4261 agaaaggcca aacagcatgc ggctgttaga gtaaaccccg ctgttataca ccgacagcga
     4321 tgccaccaga atgacgaagt tcagcgcaga agctaccaca ttgctgtcga gattatggaa
     4381 aatcatcaca aacgggctac tgttggattt cacttccacc cacggataga gtgccagtaa
     4441 aaccaccagt gaaccgatat aaaacagcag gatgcgatac accacctgat ttaccgcttt
     4501 tggaatgctt ttttccggat cgcgcgcttc agcggctgta atcccaatca gctccagacc
     4561 gccgaaggag aacataatta ccgccagcga caaaatcagc ccattccagc cggtggcgaa
     4621 gaaaccaccg tagcgccaga ggttgtcgat actggctttc tcgccgccgt gaccagaaaa
     4681 cagcagccac aggccaaagc cgatcatacc gatgatcgcc agcactttaa tcagcgcaaa
     4741 ccagaactcg gtttcgccat ataagcgcac gttcaccagg ttaacggcgt tgatgataat
     4801 aaagaaggcg gcagcccaaa tccacgttgg aacatccggg aaccagtact gcatatagat
     4861 gcccgcagcg gtcagctctg ccattcccac cagcacgaac attacccagt agttccagcc
     4921 agagaggaag cccgcaaacg gtccccagta tttataggca aagtgggcaa atgaaccgga
     4981 taccggctct tcgaccacca tctcgccaag ctggcgcata atcaggaaag cgatgatccc
     5041 ggcgacgccg tagcccagca atacagccgg acccgccatc tgaatcgccg ggccaatgcc
     5101 aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat gacggttatg
     5161 taatccgcga tgaagcgtcg gctcttgatt cgacgcagta tcttccgata cggttgacgc
     5221 gtttttcacg cctttcccct gtgtgtcttt tttgttgagg ggcacctttt aacatttagt
     5281 gcccatcgtc gcaagacaca atccacacgg ttaaacgagg tatcctgctt taattccgta
     5341 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
     5401 aaggtgcggt gatcatgccg ccaatcatcg gggcggcaat ccggctcatc acctctgaac
     5461 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
     5521 ctttcgggcg cacgcgcagg accgcgccgt gatacaacgc ctcatccagc ttctgctcgc
     5581 tgaatgtttg tggattattc aacgacggct cggcctctat ggcgtgacgt aaatacatca
     5641 gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaagccg gtacccgtcg
     5701 ccacagaaag atgaaagccc atccaccaca gtaaccagat gccgcccacc agcgcaaacg
     5761 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga tacaacagca
     5821 cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg gcgcgctcca
     5881 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
     5941 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
     6001 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
     6061 taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggcaact
     6121 ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta attggataac
     6181 gggcaatccc ttccaccctt tcgccaacca tcgccccgcc caccgcagaa gtcacaaaca
     6241 actgcacatc cgccaccgtc ataccgtaac gtgcggcttt ttcacggtta atctcaacgt
     6301 tgatatagcg cccaccttcc agccgctcgg caagcgcaga agctacgcct ggcaccgttc
     6361 gcgccacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca gtgccggaaa
     6421 ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg ttacgaattg
     6481 gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt tcctcaatga
     6541 ttttgtccat tgtcatgcct ggccgccact gatcctgtgg cttaagctgg atggtcgttt
     6601 ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt ttgccaaata
     6661 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
     6721 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
     6781 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
     6841 gcgccgccac cagcagcgtg gttttcggcc agtgcagtac tttcagcaac agcggatgat
     6901 aaacacgaat caaaaagcga ttgagcggat tactgctttc cggcggaatt ttgccacgga
     6961 tccagtaacc catcaggata gggatcacta cgatcgccag cagcgccgca cccgccatcg
     7021 catacgtttt ggtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
     7081 agatcgggat aaacgacaac gtgataatca gcagactgat aaacagcgcc ggccccactt
     7141 caacagacgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
     7201 gctggtgctg ccactcttcc agccgtttat gcgcattctc gatcatgacg atagcggcat
     7261 cgaccatcgc cccgacggca atcgcaatgc cacccagcga cataatattg gcattcagcc
     7321 cctggaaggg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
     7381 ccagcgccga gcgcacatgc cagagaaaca gcgcgcagac taccgccacc acaataaact
     7441 cttccagcaa cttgccgctg aggttgtcga tggcccggtc aatgagctgg ctgcggtcgt
     7501 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
     7561 tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc accccgcccg
     7621 ccacttcgcc ttcgccgttt aatgccccgg cgcatctccg ggccaatctg gaccttcgca
     7681 acatcgcgca gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag
     7741 tcgtcgagcg tttgtagata gccgctggcg cgcaccatat attctgcttc cgccagttcg
     7801 attgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga
     7861 ctgatgccat actgcgccag gcgctgagga tcgataacca cctgatactc tttcaccaca
     7921 ccgcctaccg acgccacttc cgcaacgtca ggaatggttt tcagctcata tttgagaaac
     7981 cagtcctgca atgagcgtaa atcggcaaga tcgtgcttac cgctgcgatc caccagtgca
     8041 tattcataga tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca
     8101 ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga
     8161 tcggtgccat cttcgaaaat gacatacaca taagagtcgc caaactgcga gaaaccacgc
     8221 acagtcttcg cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt
     8281 tcaacgattt gcggtgcctg gccgggatag ctggttttaa taatcacctg cacatcggag
     8341 agatccggca acgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga
     8401 aacaacgcgc ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca
     8461 atcattggtt attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg
     8521 cgccagaaat attggcttcg gaatcaatca ggaacaggcc gccggaaacc accttttcac
     8581 cttccgccag accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc
     8641 gtttcggtac aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ttgccggtat
     8701 caatcagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg
     8761 catccacgcc aggtagcagc gtccatttgc ggatggtgag tgtttta
//
LOCUS       DAJTYI010000145         8705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYI010000145 DAJTYI010000000
VERSION     DAJTYI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            40..648
                     /locus_tag="OO564_003405"
     CDS             40..648
                     /locus_tag="OO564_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6654400.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            746..2137
                     /gene="selA"
                     /locus_tag="OO564_003406"
     CDS             746..2137
                     /gene="selA"
                     /locus_tag="OO564_003406"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6654401.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2134..3978
                     /gene="selB"
                     /locus_tag="OO564_003407"
     CDS             2134..3978
                     /gene="selB"
                     /locus_tag="OO564_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6654402.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            4168..5319
                     /gene="yiaY"
                     /locus_tag="OO564_003408"
     CDS             4168..5319
                     /gene="yiaY"
                     /locus_tag="OO564_003408"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6654403.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            5484..7022
                     /gene="aldB"
                     /locus_tag="OO564_003409"
                     /pseudo
     CDS             5484..7022
                     /gene="aldB"
                     /locus_tag="OO564_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(7241..7396)
                     /locus_tag="OO564_003410"
     CDS             complement(7241..7396)
                     /locus_tag="OO564_003410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654404.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            7567..7890
                     /locus_tag="OO564_003411"
     CDS             7567..7890
                     /locus_tag="OO564_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6654405.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            7896..>8705
                     /locus_tag="OO564_003412"
     CDS             7896..>8705
                     /locus_tag="OO564_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6654406.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLL"
BASE COUNT         2079 a         2114 c         2423 g         2089 t
ORIGIN      
        1 gagccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt cggtagctac
       61 accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat aactttcgaa
      121 tttattaatg aactgcccta taacgcggat aacggcgtgg cgcaatttaa cccgttaggg
      181 aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc gatcatcgct
      241 gaatatattg agttaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg
      301 ttgcgggtgc gcaaaattga ggcgctggcg gatggcatta tggatgccgg gctggtatcg
      361 gtgcgtgaac aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt acgccagcgg
      421 gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa
      481 accgatacgg tcaatctggc gactatcgcc attgcctgtg ctgtcggata tctcaatttc
      541 cgccgcgttg cgccaggttg gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac
      601 ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt
      661 cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc tccgtgtgag
      721 agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct atagtcaact
      781 tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg atacttatgg
      841 tcacacccgc gtggtggaat tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg
      901 tggcagccag acgctgcctg cgtggtgtga aaactgggcg caagaagtcg atgcccggtt
      961 gacgaaagag gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca
     1021 taccaacctt gggcgagctt tacaggcgga agccgcggtg gaagccgttg cgcaggctat
     1081 gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac atcgcgatcg
     1141 ggcgctggcg cagctgctgt gccgtattac gggggcggaa gatgcctgta tcgtcaataa
     1201 caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcgggaaag aggtggtggt
     1261 atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcccgatg ttatgcgtca
     1321 ggcaggctgc accctacacg aagtagggac caccaaccgc acgcacgcga atgattatcg
     1381 tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta cataccagta actacagcat
     1441 tcaggggttc accaaagcga tagacgaagc tgaactggtg gcgctcggca aagagctgga
     1501 tgttccagtg gtgactgatt taggcagtgg ctcgctggtc gatcttagcc agtacggttt
     1561 gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg tgagcttctc
     1621 cggcgacaag ttgttaggcg ggccgcaggc agggattatc gttggtaaaa aagagatgat
     1681 cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc
     1741 ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg aaaaattacc
     1801 gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac aacgtttaca
     1861 ggctcccctt gtcacacatt acggcgcgga gtttgcggta caggttatgc catgtctttc
     1921 gcagattggc agtggttcgc tgccggttga tcgcctgccg agcgcggcat taacgtttac
     1981 accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc gtgaattgcc
     2041 agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac gctgccttga
     2101 agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc cggacacgtt
     2161 gaccacggca aaacaacctt attgcaggcg attactggcg taaatgctga ccgtctgccg
     2221 gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg cctactggcc gcagccggat
     2281 ggtcgcgtgc ctggttttat cgacgttccc ggtcatgaaa agtttctttc caacatgctg
     2341 gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg
     2401 gcacagaccc gtgagcatct ggcgattttg cagctgaccg gtaacccgat gctgacagtg
     2461 gcgctgacca aagccgatcg tgtggacgaa gcgcgtgttg atgaggttga acgccaggta
     2521 aaggaggttc tgcgggaata cggttttgct gaggcaaaac tgtttatcac cgcagcaacc
     2581 gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttgccgga acgcgagcac
     2641 gccagccaac atagtttccg cctcgcgatt gaccgcgcat ttaccgtaaa aggtgccggg
     2701 ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc actctggctg
     2761 actggtgtaa ataaaccgat gcgtgtacgt gcgctgcatg cgcaaaacca gccaacagaa
     2821 accgccaatg ccgggcagcg tatcgcgctt gacatcgcgg gtgatgcgga aaaagagcag
     2881 attaaccgtg gcgactggct gcttgccgat gtgccgccag agccgttcac acgggtgatt
     2941 gtcgagcttc aaacccatgc accgctgacc cagtggcagc cgctgcatat tcaccacgcc
     3001 gccagccacg tcacgggacg cgtttcactg ctggaagata accttgctga actggtcttc
     3061 gacaccccgt tatggctggc agataacgac cgcctggtat tgcgcgatat ctctgcccgc
     3121 aacacgctgg ccggagcgcg cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa
     3181 ccagagtatc tgcaatggct ggcttccctc gctagggcac agaatgatag cgaagcgtta
     3241 gccgttcacc tgcaacgcgg cgcggttaat cttcctgatt tcgcctgggc gcgccagctc
     3301 aacggcgaag ggatgcgcga actgctgcaa cagcctggct atattcaggc tggttatagc
     3361 ttgttgaatg cgccggttgc cgctcgctgg caacggaaaa ttctcgacac attagcgact
     3421 tatcatgagc aacatcgcga tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg
     3481 ttgccaatgg aagatgaagc gctagtactg ttgctgattg aaaagatgcg cgaaagcggc
     3541 gacatcctca gtcatcacgg ttggctgcat ctgccggatc acaaagcggg cttcagcgaa
     3601 gagcagcagg ccatctggca aaaagcagag ccgctgtttg gtgacgagcc gtggtgggtg
     3661 cgtgatctgg caaaagagac gggtaccgac gagcaggcaa tgcgcctgac gctacgccag
     3721 gcggcgcagc aaggaataat taccgcgatc gtcaaagatc gttattaccg taacgatcgg
     3781 attgtcgaat ttgccaatat gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg
     3841 gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcagat tctggaatat
     3901 tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta
     3961 ttatttccgg aaagataagg aaatgattaa ttaattttta aaataaatta atacaaattt
     4021 cttatgattt taaataaagc acattgttta atgaatacaa tgtgcttttt attagattaa
     4081 ttttgataat taataataat gatcatgttt tcgaatgaaa atcctcagta agctgcccac
     4141 ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat tccttctgtg
     4201 aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga ttatggattt
     4261 acccgtacct taattgtcac tgacaatatg ttaacgaaat taggtatggc gggtgatgtg
     4321 caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac ccaacctaac
     4381 cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagaaaataa ttgcgatagc
     4441 gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc gctggtggca
     4501 gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa accgcagctg
     4561 ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg tttctgcatc
     4621 atcactgacg aagcgcgtca taacaaaatg gcgattgttg ataagcacgt gactccgctg
     4681 ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac cgccgcaacg
     4741 ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc cacgccgatc
     4801 actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc gttagccgtt
     4861 gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt ccttgccggt
     4921 atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca gctgggcggt
     4981 ttctacaacc tgccacacgg tgtatgtaac gccgttctgc tgccgcatgt tcaggtattc
     5041 aacagcaaag tcgccgctgc acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg
     5101 acaggtaaaa acgacgcgga aggtgctgaa gcctgcatta acgccatccg tgaactggcg
     5161 aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga agatttcgcg
     5221 gttctggcga ctaatgccct gaaagatgcc tgtggcttta ctaacccgat ccaggcaact
     5281 cacgaagaaa ttgtggtgat ttatcgcgcc gcaatgtaat catcattcca acaacggctg
     5341 gcaaattgtc agccgctttt tcaactatct ctgtaatcct tgcccgtaaa ttcgtgatag
     5401 ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac ccttgttata
     5461 cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag attaagcccg
     5521 gcgagtatgg tttccccatc aagttaaaag cccgctacga caactttatt ggcggtgaat
     5581 gggtagcccc tgccgatggt gagtattacc agaacctgac gccggtgacc gggcagctgc
     5641 tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat gctgcgcaca
     5701 aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga
     5761 ttgccgatcg aatggaacaa aacctcgagc tgttagcgac agctgaaacc tgggataacg
     5821 gcaaacccat tcgcgaaacc agtgctgcgg atgtaccgct ggcgattgac catttccgct
     5881 atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg
     5941 tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg tggaacttcc
     6001 cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc
     6061 tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatt gtcggtgatt
     6121 tactgccgcc gggcgtggtg aacgtggtca atggcgcagg tggggaaatt ggcgaatatc
     6181 tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac
     6241 aaattatgca atacgcaacg caaaacatta ttccggtgac gctggagttg ggcggtaagt
     6301 cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc gataaagcgc
     6361 tggaaggctt tgcactgttt gcctttaacc agggcgaagt ttgcacctgt ccgagtcgtg
     6421 ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa
     6481 gcattcgcag cggtaacccg ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg
     6541 ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc gctgacgtgc
     6601 tcacaggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac tacctcgaac
     6661 cgacgattct gtttggtcag aacaatatgc gggtgttcta ggaggagatt tttggcccgg
     6721 tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg aacgatacgc
     6781 aatatggctt gggcgcgggc gtctggagcc gcaacggtaa tctggcctat aagatggggc
     6841 gcggcataca ggctgggcgc gtgtggacca actgttatca cgcttacccg gcacatgctg
     6901 cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc
     6961 attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg gggccgttct
     7021 gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta cgcgtcttat
     7081 catacctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat ccggcataaa
     7141 caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac ccagactacg
     7201 ccaacagaag tgcgtttctg gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg
     7261 tgagcgggcg aaccaggccg taaatcacca ttggtgatcc gaggccaatc agcaataaac
     7321 ggtagataat tgaaggttta atacgcttaa tctgtactgt ggatgtttta ctggagttat
     7381 tcattagttt ggccaatagt tccactattt caatatgata aacctcgtcc tgttgaaaaa
     7441 ccagacagca catttcagaa agtatataaa aaacaatatg tcgttatata aataaggaaa
     7501 ttgcactgca tcatggcatt tcataatatg cgttaattgt tcttttttat attctttgag
     7561 ggaaatatgt tcctggacta ttttgcactg ggagtgctta tttttgtatt tctggtgata
     7621 ttctatggga ttattatttt acatgatatt ccctacctga tttctaaaaa acgtaatcat
     7681 cctcatgccg acgccattca tgttgctggt tgggtgagtc tttttacgct gcatgttatc
     7741 tggccatttt tgtggatttg ggccacgctt taccgcccgg agcggggatg gggaatgcaa
     7801 atccatgact cctccgttgt gcaactacaa cagcgtattg ccgggctgga gaaacagctc
     7861 gccgacatca aatcctcttc tgccgagtaa tacttatgga tttactgatt attttgacct
     7921 atgtggcttt tgcatgggca atgtttaaga tcttcaaaat tcctgtaaat aaatggacca
     7981 tttccacagc ggccctgggt ggcatattta ttgtcagtgg tttaattctg ttaatgaact
     8041 ataaccatcc gtataccttc aaggcgcaaa aagcggttat ttctattcct gttgtcccac
     8101 aggtgacagg cgtggtgatc gaagtgacgg ataagaaaaa tacgctgatt aaaaaaggtg
     8161 aggtgctatt tcgactggac ccgacgcgtt atcaggcgcg ggtggatcgg ctgatggcgg
     8221 atatcgttac cgcagaacat aaacagcggg cgttgggcgc agagttagat gagatggcgg
     8281 cgaatactca gcaggcaaag gccacgcggg ataaattcgc taaagagtat cagcgttacg
     8341 cacgcggcag ccaggcgaaa gtaaacccgt tttcagaacg cgatattgat gtggcgcggc
     8401 aaaattatct ggcgcaggaa gcctccgtaa agtcatcggc ggcggaacaa aaacagatcc
     8461 agagccagct ggatagcctg gtgttgggtg aacattctca aatcgccagc ctgaaagcgc
     8521 agctcgcgga agcaaaatat aaccttgagc agacgatagt gcgtgcgccg agcgatggtt
     8581 atgtgaccca ggtgctgatt cgtccgggta cctatgccgc gtcgctgccg ctacgtccgg
     8641 tgatggtgtt tatacccgat cagaaacgac aaattgtggc gcagttccgt cagaactcct
     8701 tgctg
//
LOCUS       DAJTYI010000146         8593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYI010000146 DAJTYI010000000
VERSION     DAJTYI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..2378)
                     /gene="mdoH"
                     /locus_tag="OO564_003413"
     CDS             complement(<1..2378)
                     /gene="mdoH"
                     /locus_tag="OO564_003413"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6654407.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKL"
     gene            complement(2371..3906)
                     /gene="mdoG"
                     /locus_tag="OO564_003414"
     CDS             complement(2371..3906)
                     /gene="mdoG"
                     /locus_tag="OO564_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6654408.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4300..5457
                     /gene="mdoC"
                     /locus_tag="OO564_003415"
     CDS             4300..5457
                     /gene="mdoC"
                     /locus_tag="OO564_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6654409.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRIERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5465..6946)
                     /gene="clsC"
                     /locus_tag="OO564_003416"
                     /pseudo
     CDS             complement(5465..6946)
                     /gene="clsC"
                     /locus_tag="OO564_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(6888..7421)
                     /gene="ymdB"
                     /locus_tag="OO564_003417"
     CDS             complement(6888..7421)
                     /gene="ymdB"
                     /locus_tag="OO564_003417"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6654410.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7516..7827)
                     /locus_tag="OO564_003418"
                     /pseudo
     CDS             complement(7516..7827)
                     /locus_tag="OO564_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(7948..8280)
                     /gene="csgC"
                     /locus_tag="OO564_003419"
     CDS             complement(7948..8280)
                     /gene="csgC"
                     /locus_tag="OO564_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6654411.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8339..>8593)
                     /gene="csgA"
                     /locus_tag="OO564_003420"
     CDS             complement(8339..>8593)
                     /gene="csgA"
                     /locus_tag="OO564_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6654412.1"
                     /translation="LTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNS
                     EMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2150 a         2232 c         2033 g         2178 t
ORIGIN      
        1 agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc gatttccagc
       61 accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt aaatgacggg
      121 ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat ctcaaggaac
      181 cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag gaacagtttc
      241 cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac
      301 aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc cagccacgcc
      361 atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt gaacgcttcg
      421 ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg
      481 aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa
      541 aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt
      601 cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag caccatggtc
      661 gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc
      721 cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa tgcagtagag
      781 agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaac
      841 accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt catcaggtta
      901 ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca
      961 taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc
     1021 gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga
     1081 gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg tccccagtag
     1141 tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg
     1201 gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac
     1261 tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca taaacaatca
     1321 ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga
     1381 cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag
     1441 aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg
     1501 acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg tttggcattc
     1561 ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg gttcacgtct
     1621 tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca
     1681 actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatc
     1741 aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag gatcaggata
     1801 ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc
     1861 atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt catataccag
     1921 gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtacca
     1981 acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc
     2041 ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac
     2101 gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg atcgcgccct
     2161 tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc
     2221 gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg ttcggcatcc
     2281 agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc gcttgcggcg
     2341 atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca
     2401 ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt
     2461 ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg ggttatagcg
     2521 caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt
     2581 atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc
     2641 gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg
     2701 cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta
     2761 tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt aagcgacgat
     2821 gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttgcccca
     2881 ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat cgagatcttc
     2941 aaagcgggag aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt tttccatgga
     3001 gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt
     3061 accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg
     3121 cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc
     3181 gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac gccctggcat
     3241 aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat aaatggttaa
     3301 acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc
     3361 acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat aaacctgacc
     3421 tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc
     3481 tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc
     3541 tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac
     3601 tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta
     3661 gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg
     3721 gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt tgcttttagg
     3781 cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat caatactgaa
     3841 agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt
     3901 catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc
     3961 gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg gtatcgttca
     4021 gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc
     4081 taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta tcaaccgtct
     4141 tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc
     4201 ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga
     4261 cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa
     4321 cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat
     4381 atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc gtcattgtgg
     4441 ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt cgttatatcc
     4501 ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt
     4561 atcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt accgcaattt
     4621 attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc attgtatgac
     4681 aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagta
     4741 gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt agaaaattct
     4801 gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt atgcctcggc
     4861 atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat tctgagtaat
     4921 ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt tatcctcggc
     4981 gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt
     5041 accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta tggcagtggc
     5101 gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct gtggatggtg
     5161 aatgtggtct tctcctttgg ccaccgttta cttaacttcc agtcagcgcg ggtgacttat
     5221 tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct gtttttcggc
     5281 gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg cctgatattc
     5341 gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt actgaagttt
     5401 ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc ccgttaagcc
     5461 acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata acccgcttcc
     5521 agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga cgatcaaccc
     5581 agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac tggctctgaa
     5641 taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag
     5701 tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat
     5761 cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg ccgcgatcgt
     5821 gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg ccatagcgga
     5881 gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg ttagcggcaa
     5941 gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca
     6001 cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc
     6061 ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt
     6121 tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac gttccatcaa
     6181 ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc gtcatggcat
     6241 cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc gggacatcca
     6301 gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa tcatcggcaa
     6361 cgtcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt cgctcttccc
     6421 ctgcactaaa ataggcatca ccaatattta gtcctcccac tagggtaacc acgccatcga
     6481 cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg atatatccaa
     6541 gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg cgtggatgac
     6601 tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac
     6661 ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac cgccccgaca
     6721 tatcgtccta ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcggccagac
     6781 gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca cactgaccgg
     6841 gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca ttcatctcct
     6901 tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat
     6961 acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac tgcgatttca
     7021 gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa agccactgac
     7081 gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg caaaagctgg
     7141 tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac ggctttagcg
     7201 ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc
     7261 ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat ggccccatcg
     7321 acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt
     7381 ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc ttacaccttc
     7441 ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaacgt
     7501 gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt tctgtttatc
     7561 aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc tcattaacgc
     7621 tttgctggag taggttttgc ccggaataga accattctat gggcagttca tttctacggg
     7681 tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatcac
     7741 gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac tgccgagcat
     7801 aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta gtcatccttg
     7861 agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt ctctccagat
     7921 tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg tccattgttg
     7981 tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc gatcttccgg
     8041 ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca atgagagggt
     8101 cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca atatttgtac
     8161 tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca tatccccttg
     8221 ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata acgcattcat
     8281 attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa atgatgtatt
     8341 aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg acggaggagt
     8401 tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta acagtcattt
     8461 cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca cgttgggtca
     8521 gatcgattga gctgtcatct gagccctgac caacatctgc accattacca ccgccatgct
     8581 gggtaatagt caa
//
LOCUS       DAJTYI010000147         8474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYI010000147 DAJTYI010000000
VERSION     DAJTYI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..138
                     /locus_tag="OO564_003421"
                     /pseudo
     CDS             1..138
                     /locus_tag="OO564_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(168..3080)
                     /locus_tag="OO564_003422"
     CDS             complement(168..3080)
                     /locus_tag="OO564_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654413.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3083..3199
                     /locus_tag="OO564_003423"
     CDS             3083..3199
                     /locus_tag="OO564_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6654414.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3563..4405
                     /locus_tag="OO564_003424"
     CDS             3563..4405
                     /locus_tag="OO564_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6654415.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4408..5496
                     /gene="capU"
                     /locus_tag="OO564_003425"
     CDS             4408..5496
                     /gene="capU"
                     /locus_tag="OO564_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6654416.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5501..6451
                     /gene="virK"
                     /locus_tag="OO564_003426"
     CDS             5501..6451
                     /gene="virK"
                     /locus_tag="OO564_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6654417.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6516..7460
                     /gene="lpxM"
                     /locus_tag="OO564_003427"
                     /gene_synonym="msbB"
     CDS             6516..7460
                     /gene="lpxM"
                     /locus_tag="OO564_003427"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6654418.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7614..8474)
                     /locus_tag="OO564_003428"
                     /pseudo
     CDS             complement(7614..8474)
                     /locus_tag="OO564_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2234 a         1933 c         1997 g         2310 t
ORIGIN      
        1 gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat
       61 ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg
      121 tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga
      181 gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa
      241 cccttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc
      301 aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc
      361 tgccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc
      421 ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa
      481 ctccttcgtt tgcggcgttc cggcagcaat gcttcaggaa gatgaaccac acactctgcc
      541 agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac
      601 agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg
      661 cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt
      721 ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc
      781 agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt
      841 gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga
      901 tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc
      961 cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta
     1021 aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc
     1081 agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt
     1141 cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta
     1201 cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc
     1261 cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc
     1321 gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac attgtgtgta
     1381 gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga
     1441 tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga
     1501 ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc
     1561 gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag
     1621 catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt
     1681 gaggttttgg cattcagttc ctgctttgtc tcttccctgt cgtccagcaa ccggatgctc
     1741 ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag
     1801 cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg
     1861 taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga
     1921 cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag
     1981 agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc
     2041 atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac
     2101 tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt
     2161 tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc
     2221 agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg
     2281 cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg
     2341 ggcagccagt cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt
     2401 tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa
     2461 gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata
     2521 tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag
     2581 ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac
     2641 agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca
     2701 gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc
     2761 acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca
     2821 aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg
     2881 agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg
     2941 gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga
     3001 taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga
     3061 aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca
     3121 gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc
     3181 gctggaaatg gcgcagtagc tgcaggcctg tcatgatgcc ggaatagaag ttcgtgtaca
     3241 ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg
     3301 agcacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt
     3361 gccacctcaa ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat
     3421 gaattgttga tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat
     3481 tcatccagta tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct
     3541 tactaggtgc cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca
     3601 tgttccggta ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc
     3661 cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc
     3721 agatgaactg gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac
     3781 atttgatgat ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa
     3841 tctcaaagca catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc
     3901 tcctggaaag gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga
     3961 taatgttatg cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt
     4021 tcatgttcat acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca
     4081 gtgtaagcat cttcggcagg acttgttgag cgggagagaa tatttgaaag aaatgacagg
     4141 aaaatgcagt aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt
     4201 cgctgaagaa cttggattct attatttata tacaacagaa agaagaatga atgctcctgc
     4261 taaaggaact acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct
     4321 gaaaagacga cttttttatt atacaacacc gtttttttca tcgttgctgg cctttcataa
     4381 ggggccaaga ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat
     4441 ataggtggtc aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat
     4501 tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattggga
     4561 attgatatca cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta
     4621 ctcggaatag ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc
     4681 aatattgttg gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa
     4741 aagacatatt tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg
     4801 attgttcccg gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt
     4861 actgttgtgc ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg
     4921 cctccaaatg ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta
     4981 ggaatgttgc gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa
     5041 atgaatggac gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag
     5101 catttacagt atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat
     5161 gtttttcctg ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac
     5221 gaatcgtttg gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt
     5281 cagattggtg gaatccctga tgttattcag aacaaccaga ccgggacatt gttaccagca
     5341 ggtaataagc acgcatggat gtgcgccctg aatgattttt ttaatgaacc tgggcgtttt
     5401 tatcagatgg ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact
     5461 gcgttaaaaa tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt
     5521 atcatttatc ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg
     5581 ggaacacaga aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag
     5641 tattcgatat tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca
     5701 acccacgttg ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc
     5761 ccgggggcaa tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc
     5821 tgaatttttg tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga
     5881 ggattttgat atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc
     5941 attgtttttc aatcaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa
     6001 tggacacacc gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatgt
     6061 tattcggtgc gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc
     6121 attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag
     6181 tcatgttttc cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta
     6241 cagtgatttc tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac
     6301 acaggtggtt cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa
     6361 acgatacgct ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc
     6421 tgtaaactca gaacatcagg acttaaatta aggtttgatg tattctctga ataaaatatt
     6481 aatgatgatt ttggtagggg cattcgcact aaataatgaa aaaatacaaa tccgagttta
     6541 ttcctgaatt taagaaaaat tatctttccc ctgtttactg gtttacatgg ttcgttttgg
     6601 gaatgattgc aggtatttca atgtttcccc cttcattcag agatcctgtc ttggccaaaa
     6661 tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg cagggcgacg attaatttat
     6721 cgctttgttt cccggaaaag agtgatacag aacgggaaat aattgtcgac aatatgtttg
     6781 ccacagcatt gcaatccata gtgatgatgg cagaactggc aattcgtggt ccggaaaagt
     6841 tccagaaacg agtgttctgg aaaggcctcg aaattcttga ggaaatccgg cacaataaca
     6901 gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca atgttgctgg
     6961 cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca acgaaatcca gtgattgatt
     7021 atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt acattcccgg gaggatggga
     7081 taaaaccatt tattcagtca gtacgccagg gatactgggg gtattacctt ccagatcagg
     7141 atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa gcgacattac
     7201 caattattgg acgtctgatg aacatcagtc aggctatgat tataccgctt ttcccggttt
     7261 atgatgaaaa aaaacatttc ctgactattg aagttcggcc accaatggat gcatgcattg
     7321 ccagcgcgga caataaaatg attgcccgac aaatgaacaa aacagtggaa attttggtgg
     7381 ggtcacatcc ggaacagtat atctgggttt taaaattgtt aaaaacgcgc aaatcaaacg
     7441 aagcggaccc gtacccttga aattataaag tacaggtatt tccactagtt gtttcttaca
     7501 ggttaccaat cgaaacacat cccccttccg tgtgttgccc gtttggcacg gaggactgtg
     7561 tagatgcagt tcgttaaatt taatttatgc ctaaatagct gcgcttaata ccgctacact
     7621 tttgccagcc catgtttgcc tccggggaat gggctgacgg tttccataaa atggcgaact
     7681 tttttcaaca gttgccacat tgagcggcac tgatgattac gcgttattgt gtcgtgaagt
     7741 gcctgccata gccgttcaac atgattcacc catggcgagt aaaccggctg ataaatgccc
     7801 ctgaacttcg ggttctcctt cagccagctc tgtgtttccc ggcttttgtg gataatgtag
     7861 ttgtccacga tcagcgtgat ggttttcgcc cgacggtatg tcgctttaag ccgcttcagc
     7921 aggctgatgt aacagcgccg aacttttgct gttgccgccc acatagctga ctttacctgt
     7981 cccgctgtgc agcgctccgg ccagataata tttttcattc tgtcccggcg tgaccacccg
     8041 tttttgctgt ccgcgcagtt gccagtccgc accgattttg ggatgaagat ggatatccac
     8101 ttcatcttca taaaagaccg gatgctctgc gctgcattcg tccagtgctt tatggattgc
     8161 tgccatcttt tcatctttat gcgggtcacg gatacgcaga gttggcgcag cccttcgcca
     8221 cacaatcccc gcagacggca accagcggcg aacggttccg gcatttaact ggcaaccggt
     8281 tatctcattg atttttattg ccagcagttc tgtactccag cgtgaacgct ggtagccaaa
     8341 gtcgccggga gaatgtttta ccagctcacg taacagtgtg cagatatgct caaacggcca
     8401 gcgacgggca cgcccggcag gtaatgattt cagtccctca acacccgact gcgtgaacca
     8461 gttaatccag cgtc
//
LOCUS       DAJTYI010000148         8471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYI010000148 DAJTYI010000000
VERSION     DAJTYI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..307)
                     /locus_tag="OO564_003429"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="OO564_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(674..1192)
                     /locus_tag="OO564_003430"
                     /pseudo
     CDS             complement(674..1192)
                     /locus_tag="OO564_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1209..1894)
                     /locus_tag="OO564_003431"
                     /pseudo
     CDS             complement(1209..1894)
                     /locus_tag="OO564_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2584..4293)
                     /locus_tag="OO564_003432"
     CDS             complement(2584..4293)
                     /locus_tag="OO564_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6654419.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(4725..5444)
                     /locus_tag="OO564_003433"
     CDS             complement(4725..5444)
                     /locus_tag="OO564_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6654420.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(5569..6125)
                     /locus_tag="OO564_003434"
                     /pseudo
     CDS             complement(5569..6125)
                     /locus_tag="OO564_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6109..6367
                     /locus_tag="OO564_003435"
                     /pseudo
     CDS             6109..6367
                     /locus_tag="OO564_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6476..7054)
                     /locus_tag="OO564_003436"
     CDS             complement(6476..7054)
                     /locus_tag="OO564_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654421.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(7475..7712)
                     /locus_tag="OO564_003437"
                     /pseudo
     CDS             complement(7475..7712)
                     /locus_tag="OO564_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(7899..8328)
                     /locus_tag="OO564_003438"
                     /pseudo
     CDS             complement(7899..8328)
                     /locus_tag="OO564_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2264 a         1925 c         1634 g         2648 t
ORIGIN      
        1 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttagtgat
       61 ttgaaactgg tcccttgaga aaacattttg ctataacttt gcgctgctat agacttcctt
      121 aaaaagtttt tcacagtatt agcacagtga tctaaatgtt tttcacttat gccagaagaa
      181 gatgagtttg ttacgcgtaa tatatttttt gtaatgcttt tgttgcttta ttcggattcg
      241 tttttccatc aacaagatca atattattct gaacattaat atgatttact gcttcaggta
      301 ttttcataaa atattcccca ttatgtgcgt ttttatctat ggttatttag aatatattat
      361 ctaacttaat attttcttgt gtgtattata acgaaatgaa catattttaa tatgttgtac
      421 aatttttact ctgaaatccc ctgaaattaa tcacacctcc tttactgaaa ggaattattg
      481 caacggtatt atttgttttt ggatatatat cttgcgcctt gttatgtagt taatcctcag
      541 aatatgatag ataaaataga gacacagagc agagcgtgtt ctaacatcat gataacataa
      601 aataattagt aacaataaat gaaagaatca tgggagctca acaaaaagag agctaaatag
      661 ctgtgtggaa tagtagatca ctttggcggt ataatggcag atggtctgca tactttccgg
      721 taacaacatg ggccagaagc cccgctccgg cataactgcg tgcaatgggt tttgaaggca
      781 ccggtgcctg cacgatatgg tcgcaccggc gacaggccag tttagggcgt tgtttttcga
      841 taaccttaaa ggcgctgctg ataagctcca gctgctctga cacatcacat cccagtggac
      901 tgagttcacc accacaggcc ggacagcatt cctcttccgg ccggatgacc cggggggcac
      961 ggggaagtga ggctggtaac ggtttacggg ctgaagactg gcgcagggaa gatggcagta
     1021 ccgggtcata ttgctcaccc agtgtttccg ccatttcctc ctgaagtgcg ctgattcgct
     1081 cctgtgcttc ctgtatccgc cgttcggttt ttgcgcgaag tttttctgag cttttaccga
     1141 actgcatacg ctgcagtttc gcgaccagcg ccttcagtcg gttgatttcg gaaggcctgg
     1201 acaactgaat ctgcgaccca gacctgatta ggtgctgtgg gcgtaaagtt ctgattcagc
     1261 agattcggcg taaccggcag attgtggtca gagtttgtgg tcgccctgaa cttgcgtttc
     1321 tgcttacagt gcagcctcag ctccttacga agacgtgcca gtctgtcacg accgacgata
     1381 attccgttat ctgccagctc cgtctggagc cgccgggttc catatgtttc gcgagtgcgg
     1441 atatgtgcca ccttaatctc cagttttagc cgctcatcac tttgttttct gtctgagggg
     1501 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcgct
     1561 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
     1621 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
     1681 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
     1741 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaga gataatcggg
     1801 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa
     1861 actccgtaga gtaacgttgg ttttttctgc tcattattgg ctccttctga tgccattcta
     1921 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
     1981 tggtatatac agtttctttg agtgcggtta cgtccacaga ggtaacggta aaagtaaccc
     2041 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
     2101 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
     2161 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
     2221 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
     2281 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
     2341 gcaactggaa atactcactc tctggatgtg acctttatcc atgaagaaga tttggccaca
     2401 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
     2461 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     2521 tcactacacc ttgttggagt gaacatatca aaaacaggta attacacaat ttaaattaca
     2581 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     2641 tgaaaattgt tctgctatag tatccggttc cagattgttc tcttttatta ttctacaata
     2701 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     2761 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     2821 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     2881 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     2941 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     3001 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     3061 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     3121 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     3181 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     3241 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     3301 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     3361 tgaatatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     3421 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     3481 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     3541 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     3601 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     3661 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     3721 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     3781 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     3841 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     3901 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     3961 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     4021 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     4081 atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa aataaggtgc
     4141 tgttttattc ttaaactgaa ttagttcact ggagtttgcg accctcataa tagaacttgt
     4201 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     4261 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     4321 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     4381 atgctcctgt aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     4441 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     4501 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     4561 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     4621 tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat cgtagttatt
     4681 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     4741 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     4801 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     4861 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     4921 cgtattttat gaagtaataa cgcactatat tgcgagcatt cctgatctga ttttacatat
     4981 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     5041 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     5101 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     5161 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     5221 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     5281 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     5341 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     5401 aaagaatcta aatttagttt tagacagggc ttttttatgg gcatagaaaa cgtcctctat
     5461 aaaatagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     5521 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatgat gggaaggctt agaacgccat
     5581 attcagtttc ccatagtgga acatcactcc cagcttaaag cgctcccggt tgcggtagcc
     5641 cttggctttt attctcagca gcctgatctt gctgttcagt gcttccgcat ttccgtttga
     5701 tacgccatgg cgcatggcgt tcaggatacc gtacaacctt ttccctatcg ttttagccac
     5761 attcttcatc atggggacgt cactgttggc cgcagccatc tctgccagtc gttgcgtctt
     5821 tcctcgctcc atggtctgct ccagatatcc tttgccagtt ctttcagcgc ccagcacagg
     5881 ctcgtcagct tcatctgcgc acgcagccat atcagctttt cctgcctgga ttcggtcatc
     5941 catttatcgc tgtgctgcca caggaagcga gttccctttg tctgacgacg actttcaata
     6001 ggaagatgcg gatgttcatt ctggcgggtt ttatccacca cctcacccgg ttgtttcgcc
     6061 acatgaaagc gatcgaaggc tattttctca accgcattgg gtaagtggat gcgcgctgct
     6121 cttagtttgc tgagccagtt tagcattgcc ttctacatga ccgatgcctg agccggtata
     6181 ccgagcttta ttgtcctcaa ccagtcatgt tataagcaag aaatgcactc agcggataga
     6241 gcgacataat ctgaacctgc ggacacatct taagcggctt acccgcaaaa ccatatgctt
     6301 ctcaaagtct gatgatatgc actacaagat catcggctgg tatctgacaa tcaaccacca
     6361 ccactaaatc tgcgtcacga cctgcaactg agtctggttg aacaggctga ggccctgctg
     6421 aagcaacgcg aacctctcga ctcttgttgg attttaccaa atagtttttc taaaattaaa
     6481 cacatttata taagttcttt gctgaacaaa acgcagcccg ccatcctttt tcattcataa
     6541 attcagcaat ggcagataaa actttttcat caagcatata aaaaataatg ccagtagact
     6601 caatatgcct catacaaata aggccgcaca tatccgtaat atatttgtca atgctgctac
     6661 catttgcatg gtaatatgcc atgctattat ctgatgattt tacatatgca gtatgacata
     6721 tttttccttc acgattaaca aaactgacaa aaccactttc accaggagta atatataaag
     6781 atgaatttaa cgtgcgctca ctaatattca ttaattgttg aaattcaatg gaatagtttt
     6841 cagttaatgt atctattatc ttggctttgt ctttctttgt tatctcattg tctttttcgg
     6901 caagaaaaat tgccacttta tgacatgcat tcagctcgtt aattttacct gataaaactg
     6961 cttttcttac cacatcgttg aactcatcga tagtgtgttt tttattatat atatcatgaa
     7021 aaatatagcc taaagactta aacgagactt tcattttata ttctccttaa atattaacag
     7081 gatatatata tcacattttc ctctatattt tcagaaacca gctatcattt tccccattac
     7141 tggtacgtcg cttcagatat ttccgtagca agcgataacc ctgcgcacta tgaggatgcc
     7201 attcattata atgtttgaac accttcgcga gactacttta ctgctgttaa cacgtcaggt
     7261 tcgaacataa cactgattta atcatatttc atcgttttga cgaagttttc tgccattcca
     7321 ttttgcaccg ccgtgtgttt gggggcaagg ccgaccatct gtgtttcatt cgccagatat
     7381 aagtgatgag atggcattct ccctccttca cggcagaagc attttgaccg tttaggggat
     7441 gcggacgatt ttcatgcctg ctaagatact ttcgttactg atgctgtccg ttttccacca
     7501 tttcatcatc actgattggc gttgtgtttt tggtcagcac tccagcttta cgtttctctt
     7561 ttaaccggta actctctcct ttgatattca gcgtgtagca gccgatctag gatcgctgtt
     7621 gccagcacgt gatctccgaa catttctccc cagtctgcga cccctttgtt tgacgtcagt
     7681 atgatgctcg ctttttcata tcgacagttc atcgatagga atttaaatcc ccaaaagatt
     7741 aaaaaagcac cgaagaatgc gcgtttattc aacacaacct gatcatcaag taatcggtat
     7801 agacacttaa tattgcttac attgattgat tcaatgtatc tctgatggtt ttttgctccg
     7861 ttcccctcaa catggacgca acttccccta tgcgactctc agccgcacca ccgtattcga
     7921 gccagcggct catgcatcat aacctgctct gccagacggt aacccctctt cagccccgta
     7981 aaccgcatct gacctccgcg cagctcgcac ttcagcgggt caaccttcag tcactgctga
     8041 tacatccctc tccttgtgat ttgcctcgcc gtttttctca ctgtctccgt tatggcgtac
     8101 accacatctt ccagtaatcg ccgcttcacc ggactcagga ccccgtatca ccatccggaa
     8161 ccccttatcc gccacatgcc aggagaacct ttccataaac tcgtcgccac tcatcacaag
     8221 gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg ccaatttcat cctgaccggc
     8281 ataatgttcc aggcggctca tctgcaccgg ctgcttctcc agccagttaa gccatcggtt
     8341 tccctgctga aaaatatcgg caaaacgggg aagcatcaga aaggcggaat aacgccatcc
     8401 ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt cggcgctgat accggcagga
     8461 attgtcgcaa a
//
LOCUS       DAJTYI010000149         8449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYI010000149 DAJTYI010000000
VERSION     DAJTYI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..204)
                     /locus_tag="OO564_003439"
     CDS             complement(<1..204)
                     /locus_tag="OO564_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR6654422.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(215..2513)
                     /gene="xdhA"
                     /locus_tag="OO564_003440"
                     /pseudo
     CDS             complement(215..2513)
                     /gene="xdhA"
                     /locus_tag="OO564_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2928..3683
                     /locus_tag="OO564_003441"
     CDS             2928..3683
                     /locus_tag="OO564_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6654423.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3762..3835
                     /locus_tag="OO564_003442"
     tRNA            3762..3835
                     /locus_tag="OO564_003442"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3794..3796,aa:Gly,seq:ccc)
     gene            3970..5577
                     /locus_tag="OO564_003443"
     CDS             3970..5577
                     /locus_tag="OO564_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6654424.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            5570..6223
                     /locus_tag="OO564_003444"
     CDS             5570..6223
                     /locus_tag="OO564_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR6654425.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            7186..7914
                     /locus_tag="OO564_003445"
     CDS             7186..7914
                     /locus_tag="OO564_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6654426.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            8235..>8449
                     /locus_tag="OO564_003446"
     CDS             8235..>8449
                     /locus_tag="OO564_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654427.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFF"
BASE COUNT         2145 a         2112 c         2152 g         2040 t
ORIGIN      
        1 cgagccatct tccgccagcg taattccccg cagctccgcc agattatgaa tatcaacaat
       61 atggcgataa cggtcattgt gatggtggag ctgaatcagt acgtcagtgc caccggcgag
      121 cagtttggcc tgcgggttgt cagccagcag gttgatggca tcggcaaggg ttgctgcgcg
      181 atggtaagaa gcaaaatcaa acatgatgtt atcctcaaat caatcctgcc agatggaact
      241 cttcatataa ccgttttggc gtcagcggca gtgtattgat tgcaacaccg gtagccatct
      301 tcaccgcgtt acgaatagca gcggcaacag gaattatggg tggctcaccc agtgacttat
      361 gtccgtatgc ggattgcggc tcattgattt cgacgaacgc gctttccagt tgtggcagat
      421 ccggcatggt cggcattttg taatccagca gattggggtt acggaccacg ccgcttttag
      481 catcgatgat catctcttca aatagcgccc agccaatgcc cattcccatt ccgccgtgta
      541 cctgaccttc tgccagcagt ggattaagaa tatgccctga atcatgaacg ttgaggatgc
      601 ggttgatggt gactttgcac agcgcaatat cgaccgtcag atcaacaaag gtacagccaa
      661 acgccggtgg gttagtggtg gttttgatgg agctttcagc agagagctgc ccgccgcgtt
      721 cagggtggta gaaagcgtcc atcgccaaat cttttaacga cattaacggc tcttccggtc
      781 gttcaaccag cacgatatgg ccttttatca gggtcaaatt catcgctgac tgatgtagca
      841 tgactgcggc gtgagcgatg attttctctt ttaataatag tgccgcactg cgcagcgcag
      901 gcgcggcaac atagctctgg cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg
      961 tatcttgtgt tgaaataacg cgaacgtcgc tgaccggaac ccccacggtt tctgccacca
     1021 tttgcgagaa gacggtgtcg gcaccctgac cgatttccgt cgcgccgctt tgcacgttga
     1081 tggttccatc ctgattcatc agaaggcgcg cgcctgctat ttctacgccg acaggccagg
     1141 tgttagaggt gtagctaaaa caggcgacgc caacgccgcg gcgcaaattg ccttgctggt
     1201 tctggcattc tgcacggcgt ttttcccatt caaagatttt ccggcctttt tcaagacact
     1261 ccggcaaccc tgcgctgcaa atacgtttgc ccgtgagcgg attagcatct ccttcgcggg
     1321 cggcgttgcg taaacgaatt tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa
     1381 gcatagactc aaaggcaaat acgacttgtg gcgcgccata accacgcatc gcaccagccg
     1441 aggggaggtt ggtatagcag gtctttgaac tgtaagcgta ggcacaacga ggataaaggt
     1501 aagcgacttt attccccccc gtagaagcgg atggagtgcc cgtgagatgc ataagcgccg
     1561 gtgttagaca gaacatccag actataacct ttcaaggttc cgtcgcggtt cacgcccatt
     1621 tgcccgtcaa tagtaaaagc gtggcgggta tgggttgcga ggaaacactc ttcacggcta
     1681 agggaaactt tcaccggaat gccgccaagc ttgctggtca ggaatgccgc cattggctct
     1741 tccagtacat cctgtttatt accaaaaccg ccaccgacaa acggtttgat gactcgtacg
     1801 catgaccagg gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg
     1861 gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt
     1921 tccatgtgac aatgttgaat aacgggagtc tgatagtgcc cctgtacctg gtagtcggcg
     1981 gcatcgattg tttgttggac attgcccgtc gacatcgtgc tttgtttcag taaattgccg
     2041 ccgttatgga ttggtgcagc gtcttctgtc agcgccgctt ctggcgtggt gataacgggt
     2101 aattcttgcc actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg
     2161 gccacgacga tggcaacggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga
     2221 tcggcggtat cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga
     2281 atatcaggca catcttccca agtaaaaatc gccagtacgc ccggtaaact tctggcttgt
     2341 tcatcattaa tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcgtaa
     2401 cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg
     2461 gaatcgacgc gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc
     2521 ccctcgattt gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac
     2581 gccagaaacg acgataagcg ttaggggagg aaggaagtgt gatgacgtta aaaaaacaca
     2641 gatatgatac gaaacaggac tctgaatagg gacagtgata cgtatcaata tgagtggcga
     2701 taaaatcaat gtgataaatc aggattatta cgcatcagga tgggaaatag ggtaaaaagt
     2761 caggaacttc gatacctcta cagcgaatat tttgctgatg atacttccct ttgccaaatg
     2821 aaattaacct ctttctctat taccacgttt ttccagaagc aagagattgg gtatcaagag
     2881 gctggctgct atgatatggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac
     2941 gcctgaataa aaaatctctg ggtatcgtga tgtttttatc ggttggactg ctattggcgg
     3001 gctgttcggg tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga
     3061 aacgggggga tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagagctgg
     3121 cgcgactgaa cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg
     3181 gtggggcgaa aagtagcagt agtacacgta aatcaaccgc caaatcaacg accaaaaccg
     3241 catcggttac accgtcatca gcggtaccga aatcttcctg gccgccagta gggcaacgtt
     3301 gttggttatg gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca
     3361 ataaagggat tgatatctca gctccacggg gtacacctat ttacgccgcg ggtgcaggaa
     3421 aggtggtgta tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca
     3481 gtgaagatta cattacggct tacgcccata atgacacgat gctggtaaat aatgggcaaa
     3541 gcgtgaaggc tgggcaaaaa atcgccacta tggggagcac ggatgcggca tctgttcgcc
     3601 tgcatttcca gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgccgc
     3661 agggcagcaa gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc
     3721 taaggagagc gtaaggttta taatgcctta cgcatctcga agcgggcgta gtttaatggt
     3781 agaacgagag cttcccaagc tctatacgag ggttcgattc ccttcgcccg ctccatgaca
     3841 cttcttaaag ttcaataact tactgaatca taagggatat ggcttaacgc cttccgatag
     3901 ttttactcac tgtaaccaac actgtgacta actttgttga agtagtgagc agacctaagg
     3961 tctcagtaca tgctcaactc aagaacctac ctttatcagc gtaatggtgt cttttacatc
     4021 cgtcttagga tgaagaccac tggtcgcctt accgcttcgc ttccttctca caaccgttat
     4081 aaactagcgt cagtatcttt acggaccaag gatagacgta ccgctatggc tcactcccgg
     4141 cacatcaagt cagcacttaa agcaatccat gcagacaacc ctaacgcctc ttatgaggag
     4201 ctacgggagc acctgaagac cattgtcgag tgggaactca gtgtcagccg cgatgacctg
     4261 aacgaccctg agtcctatca gctttacgtt gaccagtacg acgatattaa gtccaacctt
     4321 cgggaagctg tagcgactga gcacctgact gtagaccagc atcgctatat caatgacgtt
     4381 atcggtgtgc ttaaagcctg tcaggataga ctcaaaggtg atagctctgg cctattgtct
     4441 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
     4501 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
     4561 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
     4621 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
     4681 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
     4741 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
     4801 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
     4861 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
     4921 tccgacgctc gtgatgcgtt cagtcctgag caggtagggc aactactggt cgctgctaaa
     4981 gcgtactctc agaagaacgc aggtaagcca taccattatt acgtcaccgc tctggctgca
     5041 atcactggag caagacttaa cgaggtggcc caacttcagg tcaaagatgt caggaccact
     5101 gaggccggga ccgtgtttat ccatatcaac gaagacgata gcagcctgcc gggtaagagt
     5161 atcaagaacg cccatagtga ccgctgtgtc ccgctggtcg atggtgctta tggttttgta
     5221 ttggctgact ttatatcact cgtagaagac cgtagaaaga ttgagggtga taatgctatg
     5281 gtattcgatg gcctcaagct gatgaagaac ggctacggtg agcaggtgag caaatggttt
     5341 aaccgaacgt tgctacctaa agtccttgct gaccgtagcg gcttagcttt tcactcgttt
     5401 agacatacag ttgcgaccca gctaaaacaa catggggtcg aattggcgta tgctcaggcg
     5461 attatgggtc atagctctgg gtctataact tacgaccgtt acgcgaaaga ggtcgaggta
     5521 gatagattgg ttaatgtgat ggcgggtgtt tataaggaga ctggagtaaa tggctgagta
     5581 ttatccggca gagtttggtg gcactgcttt taactgcccg aattgtggcg cttattctca
     5641 acagcgctgg gtccgcttaa aatatggcgc taacgggatt tatgagacac ctttcgcctt
     5701 ttctaaatgt gaacgatgca atgagaaagc ttattggtat gacgaaacca tgattatacc
     5761 agcgtcagca aacgttgaga tgcctaaccc agacatgcca gacgactgta agtccgacta
     5821 catggaggcc agaagcattg ttaacctttc tcctaaaggc gctgccgcac tcctgcgctt
     5881 aagtcttcag aagctgatgg tccatctcgg tgagccgggg aagcatatcg acacggacat
     5941 taagtcgctt gtagccaaag gattgtctcc attggtccag cgagctgctg atatttgtcg
     6001 tatcgttggc aatcaggcgg tccatccggg tgaaattaat attgatgacg accctcagtt
     6061 agcgcatggt ctttttaagc tgctgaacat tattgtcact gagcagatta ccagaccgaa
     6121 agaagtcgaa gccatgttta acagcatgcc ggaaagggcg ctcaagggca ttgaggaccg
     6181 ggacaggaag gcgagggaac agcagcaggc agctagcgag taaagcttta ggtcagattg
     6241 aatataccct cactataggg aagggggctt aactatcact ataggggaga gaccctaagt
     6301 ccccacctta ataacattca cgacccacac acattcatta agtctcaggc ttccggtagc
     6361 ggaccgattg gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt
     6421 ccctttaacg tagcaatctc tctcctttga caggcggaga acatcagacc tcagcggaac
     6481 ttccgtctat gagaccatcg gtgtgcgaga ttgtgaagaa ccaagacatc attgctgagg
     6541 tctgccatcc attgtggaca ggcggaaact caaggaggtt agaccagtgg tgtctgaggt
     6601 tgaaccttga gtcctgacca gcaggctgga ggagaccatg agtttatctt gttatggatt
     6661 gtgtttatca caattccaat aagaagaact ttaagtccag cctcctgtca tcccgaagta
     6721 aactatggtc gctatgatgt ggtctccata cttcgggtct aactggaggt cagattatgg
     6781 ccttatcagg tagcaaatct tataccctca ctatagggaa tggaccctta gctatcacta
     6841 taggggccat acattccgcc tctggtcttg atgcgtcaag ttatgcggaa gccccccgac
     6901 attaagtctt tctccatacc aaggctagca agacagcctc tgtcagactc ccagcccgtt
     6961 agcgttctcg tgtcttcatt ttgacttggg tctatcgccg ctcaccatct ggacgtcgga
     7021 catgctgtct tgcaccttta tcactactgg tcatcgcaga ccacatcaca attacctcaa
     7081 gtcattctgt ttgcgtcctg ttgcctgctg gtctcccttt acgtgggggc ttgagtaggt
     7141 cagtagggtg cgacttcagg gtgcaaacaa aaggagtaca cgtaaatgtc ccaacaagaa
     7201 atcagcgtca ttaacctcga ccagcttgtc tccatgacct cagttgaaat tgctgagctg
     7261 acaggcaagg agcaccgaaa tgtcctgcgt gatattcgca atatggctga ggagcttaac
     7321 gcgctcaaaa ctgagcagtg ctcaaaactg agcagtcata tcggggtgac tgaagatgtc
     7381 tatctaaacg cgcaggggaa acagcagccg ctgtatcgtc tcgaccgtaa gcatactttc
     7441 attctggtcg ctggctattc cgttcacctc cgcgccaagt gttacgacca tattcagacg
     7501 ttagagcgtc gagtccttca actggaagac cagaagaagc gagcagccat tcagtccgct
     7561 aaccgccgag gcgtgacttg gggcgactac tgcaaaacct atggtctgcc cgctcagaag
     7621 ctaatgaccg cacttctcca gcatcgtggc ctctttcgta agaacccaat cagtaacgag
     7681 tggtctgtaa atccgaagta ttccgactgc tttcgtatca ttaagccgtc agaccagaag
     7741 ttctcagcag gtgggtataa cttccggttc aacgctaagg ggctggaggt cttcggtaag
     7801 cctgagatgg ttgacaagat gcgaggcatc cttattgcat tcactggcac cgaccagcag
     7861 aagcaagagc acctcctgaa gctggcacaa tccggtaagg tggagggaat ctaagatgat
     7921 gattgacccg cgcacacctg aggggcgcat gactctccgt taccgtggct accggactga
     7981 agtcctcctc agagagcttg gtctagaccc ggaagatgag acccgccagc accaatcccg
     8041 agacgagcta atagcccaac tggtcgctat gaaactgtcg ctagcttgct agctgtcgtc
     8101 agcacaccgt tagtagcgcg tctactcaat acctaatttt aaaccttttg agatgaccga
     8161 tgcggggaga cgccgcagga ctgaacacgc ctgtggtcga ccgatagtct caactctcaa
     8221 gaggagtaat tactatgtta ttcaataacc aagactggaa actgtcagtc accgacatca
     8281 atctctacga gaatactgtg agtctggacg gtcagcccta tccgctgtcg ctcgctatca
     8341 agaccctcat tccgggctac ctgtcaggcc tgccgtctac cagccgcgag gcaatggagt
     8401 tactggaagc tctggctgaa gctggcgtga ccattggtaa cttcttcag
//
LOCUS       DAJTYI010000150         8340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYI010000150 DAJTYI010000000
VERSION     DAJTYI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..148
                     /locus_tag="OO564_003447"
     CDS             <1..148
                     /locus_tag="OO564_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654428.1"
                     /translation="GEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEP
                     GDDF"
     gene            complement(157..555)
                     /gene="vapC"
                     /locus_tag="OO564_003448"
     CDS             complement(157..555)
                     /gene="vapC"
                     /locus_tag="OO564_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6654429.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(555..782)
                     /gene="vapB"
                     /locus_tag="OO564_003449"
     CDS             complement(555..782)
                     /gene="vapB"
                     /locus_tag="OO564_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6654430.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            864..1283
                     /locus_tag="OO564_003450"
     CDS             864..1283
                     /locus_tag="OO564_003450"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6654431.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1270..6135
                     /gene="traI"
                     /locus_tag="OO564_003451"
     CDS             1270..6135
                     /gene="traI"
                     /locus_tag="OO564_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6654432.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6155..6901
                     /gene="traX"
                     /locus_tag="OO564_003452"
     CDS             6155..6901
                     /gene="traX"
                     /locus_tag="OO564_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6654433.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6956..7516
                     /locus_tag="OO564_003453"
     CDS             6956..7516
                     /locus_tag="OO564_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6654434.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7647..7859
                     /locus_tag="OO564_003454"
     CDS             7647..7859
                     /locus_tag="OO564_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6654435.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8103..>8340
                     /locus_tag="OO564_003455"
     CDS             8103..>8340
                     /locus_tag="OO564_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6654436.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2093 c         2671 g         1663 t
ORIGIN      
        1 cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc atccggacac
       61 ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg aacgcgggga
      121 ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt cagttctcag
      181 gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc cccgactgcg
      241 ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc
      301 tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa catcaatgcg
      361 ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc
      421 atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt tcaggttaaa
      481 acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag
      541 cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc cctgttatcc
      601 ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc tccggctggc
      661 gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat
      721 gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc
      781 attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat tgataccggg
      841 atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg
      901 ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct
      961 gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag gatgtcttta
     1021 cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg caggatggca
     1081 gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt atctccatga
     1141 tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc gtggattttg
     1201 ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac ggacggacag
     1261 tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga caccagtcgc
     1321 gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca gtataacggc
     1381 gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga gaatgtgtac
     1441 gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca ggttgaggcg
     1501 ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc gggtgtaccg
     1561 gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga agacgcctcg
     1621 ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca cgtcgatcct
     1681 gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga catccgggca
     1741 tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg acccgcttca
     1801 caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt aagtgaacgc
     1861 aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact gccgccggaa
     1921 aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagcag tgagcagctt
     1981 atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct cgatgagctg
     2041 tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt acatccggag
     2101 aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc acaggaccgt
     2161 ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga gcgggtggct
     2221 gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc tgctgaccgt
     2281 cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat aaccggacgt
     2341 cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt cgaccagggc
     2401 gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg tcataacgta
     2461 caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact gatggccatg
     2521 aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc ggccaccatc
     2581 atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt tgcggccagc
     2641 gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca ggccatactg
     2701 acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc ggaggtaacc
     2761 atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg ggatatgtac
     2821 cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga ccgctatgtg
     2881 acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca ggtcgaaacg
     2941 caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc ggaaaaaatg
     3001 ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact ccgcgtctcc
     3061 ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt tgttgtgccg
     3121 ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc actgaagctg
     3181 gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac ggtttttgcc
     3241 tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg cagtggtcgt
     3301 gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact tgcccgccat
     3361 ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac attgctggaa
     3421 accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc cattcatctg
     3481 gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct gctgacagag
     3541 gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga aatcaatgcg
     3601 cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg cacaggcctg
     3661 ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat tctcgaaggt
     3721 aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga gaaactgaca
     3781 tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt cacggtggtg
     3841 cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc agccgtgaac
     3901 atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca ccgtgcggtc
     3961 ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct gcatgacacg
     4021 cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct cgatgagagc
     4081 tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc cggtggcggt
     4141 cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg tcagcctttc
     4201 cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat tgtgcgtcag
     4261 acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga aagggcgctg
     4321 tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc atgggcaccg
     4381 gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga agcgcagcag
     4441 aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta tgaagccatt
     4501 gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat tgtcacgcac
     4561 ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga aaaggccgga
     4621 gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat acgcgacggg
     4681 gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct ggtggataat
     4741 gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct gcaggatgcg
     4801 gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt cacgctgtac
     4861 accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa gagtgaccgg
     4921 gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc
     4981 acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg ggcagagcaa
     5041 catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag tgaaaccttt
     5101 gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt tgagtcagcc
     5161 tacgtggccc tgtcgcgtat gaagcagcat gtgcaggcgt acaccgataa ccgtcagggc
     5221 tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt gtttgagccg
     5281 aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg ggagctgcgg
     5341 gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg agacagtcct
     5401 acacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact gccggcgttt
     5461 gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga tgacggaaac
     5521 gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg
     5581 gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca ggatggcgtc
     5641 cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg tgaaggccgt
     5701 ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc ggacaacagc
     5761 gtccagccgg gagccggaaa tggcgaaccg gtcacggcag aggtactggc acagcggcag
     5821 gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg
     5881 gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag ggaaattgcc
     5941 ggtcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc ggagagtgta
     6001 ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga aaatttgctg
     6061 caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc
     6121 ccgggcggag actgatacag gaagacaaac tctgatgaca acggataaca cgaacacgac
     6181 acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc
     6241 acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat
     6301 caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc
     6361 gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc
     6421 cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc tggcaggctt
     6481 tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg
     6541 gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg ccctgtgggg
     6601 gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag tcagcaaccg
     6661 gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt
     6721 tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac
     6781 ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt tctggcccgg
     6841 agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc tggcgctgtg
     6901 acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgat
     6961 agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag cgcctgtccg
     7021 cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa
     7081 gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc aggccagaca
     7141 ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata ccctgaagcc
     7201 ctggtggccg ggattatttg acggtgacac gccctggctt ctggcctgcg gtatccggga
     7261 cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag
     7321 ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg
     7381 ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc
     7441 gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac ttcaggccgt
     7501 gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa taaggcttaa
     7561 aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat
     7621 actgacaact aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc
     7681 aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct
     7741 ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg acagactggc
     7801 agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac
     7861 gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt atgagatttg
     7921 cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gggtcttttc
     7981 cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt gttattcaat
     8041 gtgtgctgca ctattcaggg gggctgaaat acaggtggca tttatctgat atggagaata
     8101 acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta ttgtctgcag
     8161 atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac
     8221 tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg
     8281 gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcacat
//
LOCUS       DAJTYI010000151         8302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYI010000151 DAJTYI010000000
VERSION     DAJTYI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            110..199
                     /gene="kdpF"
                     /locus_tag="OO564_003456"
     CDS             110..199
                     /gene="kdpF"
                     /locus_tag="OO564_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6654437.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            199..1872
                     /gene="kdpA"
                     /locus_tag="OO564_003457"
     CDS             199..1872
                     /gene="kdpA"
                     /locus_tag="OO564_003457"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6654438.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRSGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1895..3942
                     /gene="kdpB"
                     /locus_tag="OO564_003458"
                     /pseudo
     CDS             1895..3942
                     /gene="kdpB"
                     /locus_tag="OO564_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            3951..4523
                     /gene="kdpC"
                     /locus_tag="OO564_003459"
     CDS             3951..4523
                     /gene="kdpC"
                     /locus_tag="OO564_003459"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6654439.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4516..5499
                     /locus_tag="OO564_003460"
                     /pseudo
     CDS             4516..5499
                     /locus_tag="OO564_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            5499..7195
                     /locus_tag="OO564_003461"
                     /pseudo
     CDS             5499..7195
                     /locus_tag="OO564_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            7192..7869
                     /gene="kdpE"
                     /locus_tag="OO564_003462"
     CDS             7192..7869
                     /gene="kdpE"
                     /locus_tag="OO564_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6654440.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8144..8248
                     /locus_tag="OO564_003463"
     CDS             8144..8248
                     /locus_tag="OO564_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6654441.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT         1755 a         2156 c         2395 g         1996 t
ORIGIN      
        1 ctttgccatt tttatacttt ttttacaccc cgtccgcaga tttttgcgaa atctttgcag
       61 ccagaattct actcttccgg tatcactttt aggctactgg aggtgcactg tgagtgcagg
      121 cgtgatagcc ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat
      181 caatgcggag gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg
      241 gtattaatgg tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt
      301 cctcttccag gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac
      361 catgagatga actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg
      421 gcggtgctgt tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg
      481 ccggggctgt cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac
      541 tggcaatctt atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg
      601 gtacaaaact ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg
      661 tttactcgcc agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg
      721 ttatgggtgc tagtccctgt ggcgttgtta atcgcactgt tttttattca acaaggtgcg
      781 ctgcaaaact ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta
      841 cccatgggac cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc
      901 ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg
      961 cagatgctgg cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg
     1021 ggcgatcgcc gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc
     1081 gtaggcgtgg ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca
     1141 gacagcagca tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg
     1201 tttgcggtcg tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt
     1261 accgctctcg gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc
     1321 ggtgtcggtt ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc
     1381 gggctgatga ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg
     1441 aaactgactg cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg
     1501 gcgatgatga ccgacgccgg acgtagcgcc atacttaacc ctagcccgca tggttttagc
     1561 gaagtgctgt acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta
     1621 agcgccaact ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgctcc
     1681 ggggtgatta tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc
     1741 gccagctccg gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc
     1801 gtgttgctgg ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa
     1861 tatctctcct gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt
     1921 cgaaccaaca cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc
     1981 gcaatggcgc aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat
     2041 tagcatcgcg atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag
     2101 cggctggctg tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg
     2161 cagtaaagcg caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct
     2221 gcgtgagccg aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg
     2281 tgacatcgtg ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg
     2341 cggcgcatcg gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc
     2401 cggcggcgat tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat
     2461 tgagtgtagc gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg
     2521 cgcacagcga cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac
     2581 catcgtcttt ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc
     2641 cgtgagtgta acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggtgg
     2701 cctgttgtca gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc
     2761 caccagcgga cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac
     2821 cggcaccatc acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga
     2881 tgaaaaaacg ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg
     2941 tcgcagtatt gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc
     3001 gctccatgcc acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga
     3061 caaccgcatg atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg
     3121 cggtcacttc cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc aaggagccac
     3181 gccgctggtg gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt
     3241 caaaggcggt attaaagagc gcttcgccca gctgcgcaaa tgggcattaa aacggtgatg
     3301 attaccggcg ataaccgtct gactgccgcc gcgatagctg cggaagcggg tgtcgatgat
     3361 tttctcgccg aagcgacacc ggaggccaag ctggcattga tccgtcagta tcaggcggaa
     3421 ggtcgtttgg tagcgatgac cggcgacagc accaacgatg ctccggcgct ggcgcaggca
     3481 gatgtcgcgg tggcgatgaa ctccggcacc caggcggcga aagaggcggg caatatggtg
     3541 gatctcgact ctaacccgac caagttgatc gaggtggtgc acattggcaa acagatgctg
     3601 atgacccgtg gctcgctgac caccttcagc attgccaacg atgtggcgaa atacttcacc
     3661 attattccgg cggcgttcgc ggcaacatat ccgcagttaa atgcgctgaa catcatgcgc
     3721 ctgcattcgc ccgactccgc cattctcagt gcggtcattt tcaacgcctt gattatcgtc
     3781 tttttgattc ctctggcgtt aaaaggcgtg agttataaac cgcttaccgc ttctgccatg
     3841 ttgcgccgta acttatggat ttacggtctg ggtgggctgc tggtgccgtt tattggtatc
     3901 aaagtcattg atttactgct aaccgtttgt agtctggtgt gaggtttacc atgagtggat
     3961 tacgtccggc attatcgaca tttctctttc tgttattgat tactggcggc gtttacccgc
     4021 tgctgaccac cgcactgggg caatggtggt atccctggca ggccaacggt tcgttgattc
     4081 gtgaaggtga tacggtgcgc ggttcggcat taatcgggca aaattttacc ggcaacggct
     4141 attttcatgg tcgcccgtcg gcaacagcag aaatgcccta taatccacag gcttctggcg
     4201 ggagcaatct ggcagtcagt aacccggagc tggataaaca gatagccgca cgcgttgccg
     4261 cattacgggc cgctaacccg gacgccagca cgaacgttcc ggttgaactg gtgacggcgt
     4321 cggcaagcgg actggacaat aacatcaccc cgcaagcggc ggcctggcag atcccacgca
     4381 tcgcaaaagc gcgtaatctc agcgttgaac agctcacgca gctgatcgca aaatacagcc
     4441 aacaaccgct ggtgaaatat atcggccagc cggtcgtcaa cattgtcgaa ctcaatctgg
     4501 cactggataa gcttgatgaa taacgaaccc ttacgtcccg accccgaccg tctgctggaa
     4561 caaactgccg cgccgcatcg ggggaagctg aaagttttct tcagtgcctg tgcaggcgtc
     4621 gggaagacct gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg gctggatatt
     4681 gtggttggtg tggtagaaac ccatgggcgg aaagataccg ccgccatgct tgaagggctg
     4741 gctattctgc ctctgaaacg ccaggcgtac cgtgggcggc atatcagcga gtttgatctc
     4801 gatgccgccc tcgcccgccg tccggcgctg atcttaatgg acgaactggc gcacagtaat
     4861 gcgccaggtt ctcgtcatcc taaacgctgg caggatatcg aagaactgct ggaagctggc
     4921 attgatgttt tcacaaccgt caacgttcag catctggaaa gtctgaatga tgtggtcagc
     4981 ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga tgccgccgac
     5041 gacgtggtgc tggtggactt gcccccggac gatctgcgcc agcggctgaa agaaggcaac
     5101 gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa aggcaatctg
     5161 atcgccctgc gcgaactggc actgcgccgt actgccgatc gcgttgatga gcaaatgcgc
     5221 gcctggcggg ggcatcctgg cgaagagaaa gtctggcaca cgcgcgacgc gatcctttta
     5281 tgcatcggcc ataacaccgg cagcgaaaac tggtccgcgc ggcggcgcgg ctggcgtcac
     5341 ggctggggag cgtctggcac gcggtgtatg ttgaaccccc tgccctgcat cgcttaccgg
     5401 agaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga
     5461 cggcaacact ttctgatcca gcagaagaga aagcggtagt gcgttatgcc cgtgaacata
     5521 atctcggcaa gattattctc ggtcgcccag cctcgcgccg ctggtggcgt cgggaaacat
     5581 ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg
     5641 aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcatttaaa gacaagtggc
     5701 gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg
     5761 ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg
     5821 tggtggtggc gctattttat ggacgctggc cttcggtggt tgccaccgtc attaatgtgg
     5881 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     5941 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg
     6001 gcgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6061 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6121 aatttattgc ctctacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6181 aattgcagcc gttaacacat ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt
     6241 ggagttttga taaaggcctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt
     6301 accagatttt gccgctaaaa agcggcgaga aaacctacgg ctggtggtgg tggagccggg
     6361 gaatctgcgc cagttgatga tcccggaaca gcagcgcctg ctggagactt ttacgctgtt
     6421 agtcgccaat gcccttgagc ggctgacgct aaccgccagc gaagaacagg cgcggatggc
     6481 aagcgaacgt gaacagatcc gcaacgccct gctggcagcg ctttcgcatg atttacgcac
     6541 gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg caagcgaagg
     6601 atcaccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca ctacccgact
     6661 ggtgaataat ctactggata tggcgcgaat tcagtccggc ggctttaatt tgaagaaaga
     6721 gtggttaacg ctggaagaag tagtcggcag cgcgctgcaa atgctggaac cgggtttatc
     6781 gtcgcccatc aatctttctc tgccagaacc gctgacctta atccacgttg acgggccact
     6841 ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatatgcgg gtgcgcaggc
     6901 cgaaattggt atcgatgccc acgttgaggg cgaaaatcta caactggatg tctgggataa
     6961 cggccccggt cttccgccag gccaggagca gacgatattt gataagtttg ctcgcgggaa
     7021 taaagagtcg gcggtgccgg gtgtcgggct tgggttggca atttgtcggg cgattgtgga
     7081 tgtacacggc ggcaccatta ccgcgttcaa ccgaccggaa ggtggtgcct gttttcgtgt
     7141 tacacttccc cagcaaaccg ctcctgaact tgaagacttt catgaggata tgtgacaaac
     7201 gttctgattg ttgaagatga acaggctatt cgtagctttc tgcgcacggc gctggagggc
     7261 gacgggatgc gcgtctttga ggccgaaact ctgcaacgcg gcttgctgga agcggcaacc
     7321 cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg gattgagttt
     7381 atccgcgacc tgcgccagtg gagcccggtg ccggtgattg tgctttccgc acgcagcgaa
     7441 gagagcgaca agatcgccgc gctggatgcc ggagcggatg attatctgag taagccgttt
     7501 ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac gccgccactc cgccaccgcc
     7561 gcgcccgatc cgctggtgaa attttccgat gttaccgtcg atttagccgc ccgcgtgatt
     7621 caccggggtg aggaagaggt gcatctcaca ccaattgagt tccgcctgct agcggtgctg
     7681 ctcaacaatg ccggaaaagt actcacccag cgccagctcc ttaaccaggt gtgggggcca
     7741 aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg acaaaaactg
     7801 gaacaggatc cggcccggcc tcgccacttc attactgaaa ccggtattgg gtatcggttt
     7861 atgctttgaa ttgttattca ataaattcgc ttaaatagtg attaatttat tattaaacct
     7921 gcgccgaata ttattatttg gcaggtttaa tttcttaacg caaatataaa acacgaaatt
     7981 acaacattta agtaacaaaa cagcatctaa tatggcgata ttcataatta atgatttatt
     8041 atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata
     8101 gaataactct accttcaact gacacagcaa gcggtaaagg taaatggaaa ataataaccg
     8161 cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt
     8221 tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc aacgtacagg
     8281 tctccctgac tttaagcatt ac
//
LOCUS       DAJTYI010000152         8158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYI010000152 DAJTYI010000000
VERSION     DAJTYI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..910)
                     /gene="rspA"
                     /locus_tag="OO564_003464"
     CDS             complement(<1..910)
                     /gene="rspA"
                     /locus_tag="OO564_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6654442.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRR"
     gene            952..1293
                     /locus_tag="OO564_003465"
     CDS             952..1293
                     /locus_tag="OO564_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6654443.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1359..1888
                     /gene="speG"
                     /locus_tag="OO564_003466"
                     /pseudo
     CDS             1359..1888
                     /gene="speG"
                     /locus_tag="OO564_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1891..2601)
                     /locus_tag="OO564_003467"
     CDS             complement(1891..2601)
                     /locus_tag="OO564_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6654444.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2709..3014
                     /locus_tag="OO564_003468"
     CDS             2709..3014
                     /locus_tag="OO564_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6654445.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3213..3536
                     /locus_tag="OO564_003469"
                     /pseudo
     CDS             3213..3536
                     /locus_tag="OO564_003469"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4092..4691
                     /locus_tag="OO564_003470"
                     /pseudo
     CDS             4092..4691
                     /locus_tag="OO564_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5283..6272)
                     /locus_tag="OO564_003471"
     CDS             complement(5283..6272)
                     /locus_tag="OO564_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654446.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6347..6856)
                     /locus_tag="OO564_003472"
     CDS             complement(6347..6856)
                     /locus_tag="OO564_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654447.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6958..7179)
                     /locus_tag="OO564_003473"
     CDS             complement(6958..7179)
                     /locus_tag="OO564_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654448.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7426..>8158)
                     /locus_tag="OO564_003474"
     CDS             complement(7426..>8158)
                     /locus_tag="OO564_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654449.1"
                     /translation="VRNNDFVSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLV
                     GMRESKAVRRKVLAYIRQKEQEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGA
                     TPTEVIKALDLKLTSSKMLTASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPR
                     AIFNKKGETNGYYRLQPQPNDKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRL
                     VDSHLNGKATKSQKAAAGKGSDYGF"
BASE COUNT         2034 a         2056 c         2045 g         2023 t
ORIGIN      
        1 tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata taatcgatga
       61 gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca cccactgcga
      121 tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc gctggcgtcg
      181 ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc gccgcttcaa
      241 taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca aacttgttac
      301 gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac cacagctgct
      361 cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct ttcgacatgc
      421 cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg aatcccagct
      481 cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg gtatggcaat
      541 aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc atgttggcag
      601 ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac atcgtaaccg
      661 gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg atacggtgcg
      721 catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc acggaaagct
      781 cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg gtgattttta
      841 atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac gttttgcgtt
      901 cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat tatgaaaatc
      961 actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact ggcattgagc
     1021 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
     1081 cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct gcgccagaaa
     1141 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga taaccgcgat
     1201 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt gcgctgcctg
     1261 gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag gaatgtaagg
     1321 acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa gatttacgct
     1381 atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag gaaccctacg
     1441 aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc agagcgaacg
     1501 gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg tggaaattaa
     1561 ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc aggggaaagg
     1621 tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc tcaatctcta
     1681 taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt accgcaagct
     1741 tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc aatatcgtaa
     1801 tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa caccgggtca
     1861 gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat ttttgatggt
     1921 gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct gacagtccac
     1981 cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc gttgattatt
     2041 tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg gcgtggcgct
     2101 gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc cctgatcatc
     2161 atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc cattatcatc
     2221 ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc cctgtaaacg
     2281 tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta ccagagcgac
     2341 cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt cttccgacaa
     2401 agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct gagtgaaatc
     2461 ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg ccatctcagg
     2521 ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg cgagtaagca
     2581 tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct caatcatagt
     2641 agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct tcgcgtaagg
     2701 agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc gcctcacccg
     2761 cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca cagcgcatta
     2821 tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat gaccaggttg
     2881 atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa attctctaca
     2941 accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat ggcgcgcctg
     3001 ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg gcgatttgaa
     3061 catacaacat aatctcacct tattactcat acccttctat tgatgtggat taataattct
     3121 taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa tatataaaca
     3181 tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga atggtgggca
     3241 tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg gctgcaggcg
     3301 gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag gccagcgaaa
     3361 aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt cgtctacatg
     3421 ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat gagtacggca
     3481 accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt aatcatcccg
     3541 atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa ttcgaacgga
     3601 ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc gtcgaacagt
     3661 atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc aatatgaccc
     3721 gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc ggttcactca
     3781 atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac acctacggca
     3841 gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg atgtttggta
     3901 acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt gaaaaagcgc
     3961 gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat acggctgccg
     4021 gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg gttgcgggta
     4081 ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat aaatactgcg
     4141 tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac tataaagcct
     4201 atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct tcgcaaatta
     4261 ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca acaaaacccg
     4321 cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg actgcacgcg
     4381 ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga aatagtggcg
     4441 cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat aatccggtca
     4501 aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg caaatgacgg
     4561 cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag ttcatctgga
     4621 actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt aggcctgagg
     4681 tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc attagacggg
     4741 atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag ggtggcaccg
     4801 gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc caaaactcat
     4861 cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca ccagcaccag
     4921 cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc ctgtgtctat
     4981 tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc ccagaccata
     5041 gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca gtccagcctt
     5101 acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg gtgccgcatc
     5161 aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg atgtaccacc
     5221 accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct tacccattgc
     5281 tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac tgcggttgca
     5341 gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac cttaccgagg
     5401 atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt aatattctca
     5461 ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag catgaacaca
     5521 cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac gatagcgtct
     5581 tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg ggactcatgg
     5641 gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt ctcaaacaga
     5701 gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg cagctctacg
     5761 gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat ggtacggacc
     5821 acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa accagtggag
     5881 gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc gataggttcg
     5941 acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc accgcgtaca
     6001 gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg caggctattg
     6061 cgggtaggca ggacttctac agggaacgga atgttatcct gagcgtcttt aagtacaccg
     6121 ttcagcatag ccttaaccgt cttagctaac tggagggcat agacctgtgg gtcagcgtta
     6181 gttggcagac cttcgttaac ctgaatgttg tcggtgtcgg tgatacgtac tggctcaatg
     6241 ccgaactgat agctatcttt aatcatggtc ataataatca atctcctaaa ttagttttag
     6301 atactgtggg tcccgacatc aggtcaggac cacttgtgtg tctgtcttag aggctgtcca
     6361 tccagttacc gcctgactca atgtcagcga caatagcggc acgggagcga ccagcggaat
     6421 gggtagcact gcggaggttc tcaccatagg tccggcgctc agctttgacc tgttcgcggc
     6481 gctgtagctc tttctgccac tctgcatcgg tcatgttctc ttcacgcata cgctcagcct
     6541 gaatgcgctg ctctttagcg atacgctcag cctcggctcg cttgttctgc tggcgacgtt
     6601 ggtaggcggt catcaggtca ttgctgaaga agtttccaat ggtcacgcca gcttcagcca
     6661 gagcttccag taattccatg gactcgcggc tggtggcagg cagtcctgac aggtagcccg
     6721 gaatcagggt cttaatagcg aaggacagtg ggtaggactg accgtccaga ctcacagtgt
     6781 tttcgtagag attgatgtcg gtgacatgga gtttccagtc attgttgttg aataacgcac
     6841 tagctttgct agacatagta attactcctc ttgagagttg agactatcgg taccgcagac
     6901 acagttatgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa ttaggggtta
     6961 gtgagtggcg tccagcttag cgaacgctcg gcgtaactga ttgttcaggt cacggtcgac
     7021 cagcaggtct atcagttcgt ctcgactata gaaaggacgc tcagggtcct ctacgcccat
     7081 ccccagcatt gacaggaggc gacgggtagg cagaccacgg taacggagag tagcctttcc
     7141 gatgggtgtc cgtgggtcga tatgttgttt agttgtcatt gttagaagtc ctcgttagac
     7201 caatcatcca cgtcaaattc actggtgata ggagcttgga agttaggtag accctctggt
     7261 atctctagag gcagtcgctc gtcgactctc agtgccatct cttcgtgtat atgccgaagc
     7321 agagaagtca gtaagatagg gtccgcagga tgctcagggc cgtgtctctc aatgaaacat
     7381 atggttgtcg ctattgactc cataatcagt tcatcagtga gtcgcttaga acccatagtc
     7441 agaccccttg cccgctgctg ctttctggga ctttgtagcc ttaccattca ggtgagaatc
     7501 caccagacga cggacaacct tgatattgga tgtgcgcatg tcatgagcga tatccttcca
     7561 gttatccttc agccagtcat aacccttgcg tagaatcttg atggtaggct ctttgtcgtt
     7621 aggctgtggc tgtagacgat agtagccgtt ggtctcacct ttcttattga agatggctcg
     7681 tggacgtccg gtcgataggt ctatatccgt agtgccaaac tcagtaacac gcttggtgta
     7741 gaatgggtta ggacgacaca gggtcttcag atatccagtc aggacgctag ctgtcagcat
     7801 cttagagctg gtcaacttaa ggtccagagc tttaataacc tcggtaggcg tggcgcctga
     7861 cttggagtcc agcttggagt cgaggacgat agcaccgggc gcatagcaga tagctttggt
     7921 ctgtgcgacc tgaagttgct gggcctgaag ttcgaggacc tcttgctcct tctggcgaat
     7981 ataggctagg accttacgac gcacagcttt agactcacgc atcccgacca gcagaagctg
     8041 gtcagtgttg agggtatagc ttttaacgtc ctgcccgaag cttacgaaaa tttcgtaggc
     8101 tacattttga ccgtccagct catcttcaat acgggacaca aagtcgttgt tacggaca
//
LOCUS       DAJTYI010000153         8133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYI010000153 DAJTYI010000000
VERSION     DAJTYI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..894)
                     /locus_tag="OO564_003475"
     CDS             complement(<1..894)
                     /locus_tag="OO564_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6654450.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OO564_003476"
     CDS             954..1232
                     /locus_tag="OO564_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654451.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO564_003477"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO564_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6654452.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO564_003478"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO564_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6654453.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OO564_003479"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OO564_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6654454.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OO564_003480"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OO564_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6654455.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OO564_003481"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OO564_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OO564_003482"
     CDS             complement(4912..5442)
                     /locus_tag="OO564_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6654456.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO564_003483"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO564_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6654457.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFAAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OO564_003484"
     CDS             complement(6411..7634)
                     /locus_tag="OO564_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654458.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2361 a         1574 c         1703 g         2495 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgctc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg cagcttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaactagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta ttgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcatttatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaattaat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttggta gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgt ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg cacccaagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcagc aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaagcca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcggt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcataaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctagggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt ttt
//
LOCUS       DAJTYI010000154         8103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYI010000154 DAJTYI010000000
VERSION     DAJTYI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..1325)
                     /gene="phoA"
                     /locus_tag="OO564_003485"
                     /pseudo
     CDS             complement(1..1325)
                     /gene="phoA"
                     /locus_tag="OO564_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1426..1686)
                     /gene="iraP"
                     /locus_tag="OO564_003486"
     CDS             complement(1426..1686)
                     /gene="iraP"
                     /locus_tag="OO564_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6654459.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(1868..2071)
                     /locus_tag="OO564_003487"
     CDS             complement(1868..2071)
                     /locus_tag="OO564_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654460.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2149..3243
                     /gene="ddlA"
                     /locus_tag="OO564_003488"
     CDS             2149..3243
                     /gene="ddlA"
                     /locus_tag="OO564_003488"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6654461.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3267..3479)
                     /locus_tag="OO564_003489"
     CDS             complement(3267..3479)
                     /locus_tag="OO564_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6654462.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3739..4047
                     /locus_tag="OO564_003490"
     CDS             3739..4047
                     /locus_tag="OO564_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6654463.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4106..5200)
                     /gene="yaiW"
                     /locus_tag="OO564_003491"
     CDS             complement(4106..5200)
                     /gene="yaiW"
                     /locus_tag="OO564_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6654464.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5213..6433)
                     /gene="sbmA"
                     /locus_tag="OO564_003492"
     CDS             complement(5213..6433)
                     /gene="sbmA"
                     /locus_tag="OO564_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6654465.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6785..7942
                     /gene="ampH"
                     /locus_tag="OO564_003493"
     CDS             6785..7942
                     /gene="ampH"
                     /locus_tag="OO564_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6654466.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(7943..>8103)
                     /locus_tag="OO564_003494"
     CDS             complement(7943..>8103)
                     /locus_tag="OO564_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6654467.1"
                     /translation="LRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVA
                     INRLPSEY"
BASE COUNT         1999 a         2096 c         1952 g         2056 t
ORIGIN      
        1 cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggaat tcccgtaact
       61 catcaccatc actgcgccat ctttagtatt tagcgcctga gtgaggcccg gcgctttggt
      121 atccggcgca acaacctggc tggcgtgggc gtgatcagcg gtgactatga ccagcgtgtt
      181 accctccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat cgaccgtctc
      241 gccaatttgc ccacaaggat tcgcagcgtg atcctgttta tcgattgacg caccttcaac
      301 ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt cggtcatctg
      361 tgccagggtt ggtacactgt cattacgttg cggattaggc gtataggtga ctgcgggctt
      421 atcgatattg ccgtggtacg ttgctttcgg ccctagccag cgcactggca tattgccgtc
      481 agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat tcagtgaggc
      541 agcatcgttc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg tttttccctg
      601 ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa gcgtaacgtc
      661 ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt ccagagcgtt
      721 acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgaag tcacatgtgc
      781 caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac cggtcgccag
      841 acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa tatcgacgcc
      901 cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg cagccgagtc
      961 ggtgacgtag tccggtttgc cagtttttta ttcagcgcat agtgagtgta ttgcccggta
     1021 agcggtaagg catcgatacc tttaaaaaag ccgcccgcac cttcggcata attacgtgcg
     1081 gcagtaattt ccgaatcccc catcccatcg ccaatcagca aaataatatt ttttgcaggt
     1141 ttatcgctaa gagaatcacg cagagcggcg gtctgatcac ccgttaagcg gcgagcaccg
     1201 ccgggtgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat ttctggtgtc
     1261 cgggcttttg tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat agtgctttgt
     1321 ttcactttat tttctccatg tacaaataca ttaaaaaata aaaacaaagc gactataagt
     1381 ctcggccgtg acaactttat gacagctatt gaaaagatta actttttact gacgaggatg
     1441 cttcaataac ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag gaatgctggc
     1501 atcgggcttt acctcgtaca gcgccccctc tacctgatca atcaaccgct gttggtcatt
     1561 ttgcgccata ttgcgaagca ttgcagtgac gataatctcc aaagcttcta cctgcgcaca
     1621 cagttctttc gactcttctt ctttttgggc aagcttaaat aacaactcag taatgagatt
     1681 tttcatgtct gtatttcctt atccaaagta tggagaagtt aacattatgt ttcacaactt
     1741 catagtgtct ggaagtatta tttttatgac aaaggaaata ctttgcgcaa atattttttg
     1801 tttgattacc agcgaaacgt ttctcttatt taaataatta cccacacaaa atataggctt
     1861 ttaattatca acctttatta taaataaaag ctatgaaata tattctcttc gttaacttat
     1921 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
     1981 tcatcatttt tgtgatcttg cgcacatctt tgagataacg cactttcttc accagtacgt
     2041 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
     2101 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
     2161 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
     2221 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
     2281 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
     2341 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
     2401 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
     2461 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
     2521 ttaccgtttg taggttctga tgttctggct tcagcaacct gtatggataa agatgtcacc
     2581 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
     2641 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
     2701 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
     2761 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
     2821 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
     2881 ggcgagctcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     2941 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     3001 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     3061 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     3121 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     3181 cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa aaccacaatg
     3241 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     3301 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     3361 gtctgccaac cttttgcttc aaatcccagt acccccacca cgccattaag aatgaatata
     3421 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     3481 gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta
     3541 gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg tgactcacaa
     3601 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     3661 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     3721 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     3781 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     3841 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt
     3901 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     3961 attttcgggc tgatcccgtt tttagcaggc tgcctcattt ttgcagtggt ggcgctatgg
     4021 ctgcactggc gacatcgccg ccagtaaaat ttacgttgcc agatgcggcc tgaaacgcct
     4081 tatctggcct gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     4141 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     4201 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     4261 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc
     4321 gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     4381 tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     4441 tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     4501 aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag
     4561 aaccaaacca caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt
     4621 tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac
     4681 cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca
     4741 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     4801 aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt
     4861 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg
     4921 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     4981 ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc gcccaggcat
     5041 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt
     5101 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     5161 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     5221 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     5281 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     5341 caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac
     5401 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     5461 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta
     5521 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     5581 aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca
     5641 gtttgatccc taccactgcc agcaatccgg tccccatcag cgaccagacg atagcggcaa
     5701 tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg
     5761 ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca
     5821 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt
     5881 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     5941 ggaacacgta gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg
     6001 caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg
     6061 aacttagcgc ggtttgaatc agatcataga acggcgcata ccaggcgttg acggcgaccc
     6121 cgacttccac caaaaaccaa gtgacgaaga taatcagcgc agtaccgagt attgaccagt
     6181 attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa
     6241 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     6301 tctgcccgga agcgccggtg atacgtgcca cccagtcacc cccaccggct tgccagaaga
     6361 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgttcccggc tttgggaaaa
     6421 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     6481 accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     6541 gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat
     6601 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcagtgg tgagcaggaa
     6661 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     6721 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     6781 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     6841 ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     6901 cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta tcgacggtaa
     6961 ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     7021 ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa tgctggtgaa
     7081 attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg ctccgccagg
     7141 cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg caacccatac
     7201 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc
     7261 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     7321 tcaggcggcg tactctaacc ttgcgttcga cctactggca gatgcactgg cgaatgcgtc
     7381 cggcaagcct tatacccagt tgtttgaaga gcagattacc catccattgg ggatgaaaga
     7441 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     7501 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg
     7561 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     7621 ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat
     7681 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     7741 gggtcgtccg aggattattc agaagacagg cggtggtggc ggtttcatta cctatatggc
     7801 gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     7861 ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg ggaataaacc
     7921 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     7981 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga
     8041 cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata cgtgatcgca
     8101 att
//
LOCUS       DAJTYI010000155         8084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYI010000155 DAJTYI010000000
VERSION     DAJTYI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            327..3176
                     /locus_tag="OO564_003495"
     CDS             327..3176
                     /locus_tag="OO564_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6654468.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3276..5195)
                     /gene="dhaR"
                     /locus_tag="OO564_003496"
     CDS             complement(3276..5195)
                     /gene="dhaR"
                     /locus_tag="OO564_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6654469.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5423..6493
                     /gene="dhaK"
                     /locus_tag="OO564_003497"
     CDS             5423..6493
                     /gene="dhaK"
                     /locus_tag="OO564_003497"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6654470.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6504..7136
                     /gene="dhaL"
                     /locus_tag="OO564_003498"
     CDS             6504..7136
                     /gene="dhaL"
                     /locus_tag="OO564_003498"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6654471.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7147..>8084
                     /gene="dhaM"
                     /locus_tag="OO564_003499"
     CDS             7147..>8084
                     /gene="dhaM"
                     /locus_tag="OO564_003499"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6654472.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAA"
BASE COUNT         2103 a         2006 c         2025 g         1950 t
ORIGIN      
        1 ctaattagga ttattacaaa aaatcttcac caaatttttt aaccgctgtt tgttgtaata
       61 tttttgccat attcatagta acgaattgga ataagtaacg acaacttagg gaataactcg
      121 atataaactc acctttgtaa ggggggtgtt aaatatatat taattcctgt gttcctgatt
      181 ttgcccggta atagagaatt attctcagag aagctcatca tgattgcacc atacagatat
      241 catgatgata agaggatatt attcattgac ttaaacaaaa tgacaaaatc catagaccca
      301 tagcctgggt actatatagg agtggtatgg gcatcaaaca acacaatggg aataccaaag
      361 ccgatcgtct cgctgaatta aaaatccgtt cgccctcaat tcaactgata aaatttggcg
      421 ctattggttt gaatgcaatt atcttttccc ccctgctgat agctgctgat acaggaagtc
      481 aatatggcac caatattact attaatgatg gtgacagaat tacaggagat accgccgatc
      541 catcaggaaa cctctatggt gtaatgaccc cagcaggaaa cacgcctggc aatatcaacc
      601 tgggtaatga tgtcaccgtc aatgtcaacg acgcctctgg atatgcaaaa ggaatcatta
      661 ttcagggcaa aaacagctcc ctgacagcta accgactcac agtagatgtt gttggtcaaa
      721 cctctgccat cggcattaac ttaattggtg actataccca tgctgactta ggcacaggca
      781 gcaccattaa gagtaacgat gacggcatca ttattgggca tagctcaaca ctaacagcca
      841 ctcaattcac cattgaaaac tcgaacggta taggcctaac catcaatgac tatggcacca
      901 gtgtcgatct tggaagcgga agtaaaatca agaccgatgg aagtacaggt gtttatatcg
      961 gtggtctcaa cggcaataac gccaatggtg ctgcgcgttt tacggcgaca gacctgacaa
     1021 tcgatgttca gggctacagc gccatgggga taaacgtaca gaaaaactct gttgtcgatc
     1081 tcggaacaaa cagtaccatt aaaaccaatg gcgataatgc tcacggcctc tggagctttg
     1141 gccaggttag cgcgaatgca ctcactgttg atgtaactgg agccgcggcc aatggcgtcg
     1201 aagttcgtgg tggtacaacc actatcggtg cagatagcca tatttcttcc gcgcagggcg
     1261 gtggcctcgt caccagtggt tcagacgcga caatcaattt ttctggcacg gcagcgcaac
     1321 gaaacagcat cttttccggc ggttcttatg gtgcctcggc ccagacggca acggctgtta
     1381 tcaacatgca aaataccgat attacggttg atcgtaatgg cagtctggcg ctgggtttgt
     1441 gggcgctcag cggcggtaga ataaccggag acagtttggc tatcaccggc gcggcaggag
     1501 ccagagggat ttatgccatg accaacagcc agatcgacct cacgagcgat ctggtcattg
     1561 atatgagtac acccgaccag atggccatcg caacgcaaca tgacgatggt tatgccacca
     1621 gccgcatcaa cgcctcgggt cgtatgctta tcaacggtag cgttctttcc aaaggtgggc
     1681 taatcaatct ggatatgcac cctgggtcgg tttggacagg ttcctccctc agcgataatg
     1741 tcaatggcgg gaaactggac gttgcaatga ataacagcgt ctggaacgta acaagtaatt
     1801 ctaatctcga cacgctggcg ctgagccatt caactgtcga ttttgccagc cacgggtcaa
     1861 ctgccggcac atttaccaca ttaaacgtag agaacctgag cggtaacagt acctttatta
     1921 tgcgtgctga tgttgttggc gagggtaatg gcgttaataa tagaggggat ttattgaata
     1981 tcagcgggag tagtgctggt aatcacgtat tggctatccg caaccagggc agcgaggcca
     2041 caacgggaaa tgaagttctg acagtggtaa aaaccactga cggcgcggcc tcgttcagcg
     2101 cgtcttctca ggttgagttg gggggatatc tgtacgatgt gcgtaaaaat ggcactaact
     2161 gggagcttta cgcttccggg acagttccgg aaccgactcc taatcctgaa cccacaccag
     2221 ctcccgctca gcctcccata gtcaaccccg atcctacgcc taaacccacc acgaccgcag
     2281 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
     2341 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
     2401 gttatggggg aagcctggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
     2461 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
     2521 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
     2581 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
     2641 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaact
     2701 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
     2761 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
     2821 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
     2881 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
     2941 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
     3001 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
     3061 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
     3121 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
     3181 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
     3241 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
     3301 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
     3361 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
     3421 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
     3481 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
     3541 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
     3601 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
     3661 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
     3721 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
     3781 accagcattg cgaggtccgc ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
     3841 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
     3901 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
     3961 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
     4021 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
     4081 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
     4141 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
     4201 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
     4261 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
     4321 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
     4381 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
     4441 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
     4501 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
     4561 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
     4621 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
     4681 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
     4741 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
     4801 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
     4861 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
     4921 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
     4981 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
     5041 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
     5101 catgtctccc gtttcatcag cttattgcat cgctcccagg aggttgcaat taagggagat
     5161 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
     5221 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacatttat
     5281 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
     5341 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
     5401 ccgtaattgc tggcgcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
     5461 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
     5521 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
     5581 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
     5641 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
     5701 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
     5761 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
     5821 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
     5881 cgtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggacgcctg
     5941 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
     6001 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
     6061 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
     6121 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
     6181 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
     6241 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
     6301 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
     6361 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
     6421 gaaagttgat gacgaaacgc tggctctctg ggacgtcccg gtccacaccc cggcccttaa
     6481 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
     6541 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
     6601 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
     6661 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
     6721 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
     6781 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
     6841 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
     6901 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
     6961 tggcgctcga agctgccagt agcatcgccg aatccgcggc acaaagtacg attacgatgc
     7021 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
     7081 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
     7141 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
     7201 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
     7261 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
     7321 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
     7381 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
     7441 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
     7501 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
     7561 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
     7621 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
     7681 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
     7741 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
     7801 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcagctggc tgaagataac
     7861 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
     7921 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
     7981 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
     8041 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgc
//
LOCUS       DAJTYI010000156         8077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYI010000156 DAJTYI010000000
VERSION     DAJTYI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(82..630)
                     /locus_tag="OO564_003500"
     CDS             complement(82..630)
                     /locus_tag="OO564_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6654473.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(630..1337)
                     /locus_tag="OO564_003501"
     CDS             complement(630..1337)
                     /locus_tag="OO564_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6654474.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1352..2029)
                     /gene="ydjY"
                     /locus_tag="OO564_003502"
     CDS             complement(1352..2029)
                     /gene="ydjY"
                     /locus_tag="OO564_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6654475.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2034..2744)
                     /locus_tag="OO564_003503"
     CDS             complement(2034..2744)
                     /locus_tag="OO564_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6654476.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(2911..3717)
                     /gene="xthA"
                     /locus_tag="OO564_003504"
     CDS             complement(2911..3717)
                     /gene="xthA"
                     /locus_tag="OO564_003504"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6654477.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4163..4741
                     /locus_tag="OO564_003505"
     CDS             4163..4741
                     /locus_tag="OO564_003505"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6654478.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4757..5383
                     /locus_tag="OO564_003506"
     CDS             4757..5383
                     /locus_tag="OO564_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6654479.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5380..7113
                     /gene="astA"
                     /locus_tag="OO564_003507"
     CDS             5380..7113
                     /gene="astA"
                     /locus_tag="OO564_003507"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6654480.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7110..>8077
                     /gene="astB"
                     /locus_tag="OO564_003508"
     CDS             7110..>8077
                     /gene="astB"
                     /locus_tag="OO564_003508"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6654481.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLP"
BASE COUNT         1901 a         2073 c         2202 g         1901 t
ORIGIN      
        1 ctgccagtca ctggcatgtg ttgccaaaga aaacagcgat aacaatccca gcaaccaccc
       61 acaatggcgc atcttccctc cttaagaacg tttttccgga atacggcaca gcccctgagc
      121 gggaatgtcc atggcagaat taaaacgagc cgacaggttt tgcaggccga tcaatgccgt
      181 cagttcggtg agcgcctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc
      241 gactgttggc ggtgtgacgc ttgcggcttc ggtgtactcc agcgccagcc gttcttcatc
      301 gctaaagagc gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt
      361 acgctcggcg actttcatac tggtgatatc cacgcaaaac tcacacaggc acatttgcgc
      421 aatgcgcgcg ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag
      481 ccacccaaca aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat
      541 cgggcttagc acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa
      601 aggagttttg ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat
      661 attttcttcg ccataaaaat caccacggtc agggcaaaca gaataaacag ttcggtgaca
      721 aaccagaaag taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt
      781 aattgaccaa gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca
      841 gcatagctga ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt
      901 ttgccgtagc gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt
      961 tccaccactt cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc
     1021 gaactggtcc acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag
     1081 gtgatcaaaa acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag
     1141 acaactgccg ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg
     1201 gcggcaaacg ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat
     1261 gcgagcagca tagcgaacaa caggatgatg agggttatac ggtagtacca gattttacgc
     1321 gattgtatct tcatcatcct ttatcctggc tttattcggt gattttaaag gtaaccgttg
     1381 ccaacgtgtt atccgccggg agaactgagg cattgccttt gaatttcact tcgccgcgtt
     1441 tttccaccgc accataagtg tatgttgcat tgctgacgat gccgaccggg cagctatcca
     1501 gacacaccag gcaacccgtt ttcttctctt ctgctgccgt taaattaccg ccaaagcgca
     1561 tgtccagttt cttgccatta ctgtcaacaa tcacttcatc gaaggaatac gcttttgccg
     1621 ccccttgcca gttgaccgaa atatccagtt tgctgcctgt gacatgagtc gtttctttat
     1681 tatccatcgt catgttttcg cccggcgtac cacctgcctc tttcagggct tcataaaatg
     1741 ctttcggggt cgcataagcc ataaacagcg atttatgtcc gttggagcca tctttaaaca
     1801 caataccgtg acgagtgtcg tcggtgagga aacgaccatt aatttgcacc agcatggtta
     1861 ccgtatggtt attggcatcg acgcgcagcg gttggctgtt cgaaagtccg tcgtattcca
     1921 cttttgccgc cgcattctct ttttgatcgc agccgctaag cccggcaaca gccagtaagc
     1981 agagtgcagc cagtcctttt ttccatgaca ctgaataatg ttgcgacatc gttctatcct
     2041 tcattttttg ggtgagtaag tgggctgcgg cgcgaagcag acagatccac atgtttgtgt
     2101 cgggcgtaga gttttgcgag ctggatgaga ataaacagcg ccaggcccgc cagacagagt
     2161 tgtaaaataa agcgcagcgt aatgccttca ttggcgagat cgcttgccat cacggtataa
     2221 ataacaatac cgggcagggt cgtgagtgcc gaaataaggg tataaggcca gaaggcgatg
     2281 gtggttaatc cgtaagcgta attttgaata ttgtaaggaa acaacgggat taagcgggtc
     2341 agaataagaa aatcaatacc gttacgcgca atgccttttt caatggcctg gaaggtatgg
     2401 ctatggccca cgtatttcag cagtaaatca cgtcccagcc agcgcgccag caggaatgag
     2461 cacgaggagg ccagcgttgc ggcaattaat gagagtagtg tccctaagag cgggccaaaa
     2521 actattcctc cagcgatcac cagtatgctt cctggtaaca gaaagagggg ggcattgatg
     2581 aataacagaa tatagaggtt ataaccgaac aatccgctct ggcgaatgag tgtctgtaag
     2641 tggggtaaat cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta aatgaccagc
     2701 gcaaaaataa gacaggcaaa cagaaagtta cgctcagcgt tcatcgtgga gtttcctata
     2761 tcaaacttga taatagagaa gtggtcagac aactaaaaac gcagtgacgg tataacaaag
     2821 gacggcaggc aacaaatcat tactgacagt ttgttgattt ttctcaaggt taattctcct
     2881 gacccagttt gagccaggag agctgctaaa ttagcggcgg aaggtcgccc agacgggggc
     2941 gtgatcggac ggtttttcca tgctgcgaat ttcatagtcg atgccggttt ctacgcaaca
     3001 ttctgccagc ggttggctgg cgagcagcag gtcgatgcgc agaccacggt tatcgtcaaa
     3061 accttttgag cggtaatcaa accatgagaa acgatctgct gtttgcggat tagcatggcg
     3121 gaaggtatcg accacccccc agctcatcag cctgtccatc cattcgcgct cttccggcag
     3181 gaaagagcat ttaccggtac gcagccagcg cttacggttt tcttcgccaa tgccgatatc
     3241 cagatctgta gggctgatat tcatatcgcc cataatcagt accggattat cacgtttgag
     3301 ttcggtttcc aggtagtttt gcagattctg ataaaactgc gcttttgccg ggaattttat
     3361 cggatggtcg cggctttcac cctgcgggaa gtaaccgttg atcacggtga cataacccag
     3421 cagtgagggg atttccgcca taataatccg ccgctgcgcc tcttcgtcgt caccgggaaa
     3481 gccgcgacgc acggcaatcg gcgtctcttt ggtcagcagc gccacgccat aatggccttt
     3541 ctgcccgtga taaaacacgt tgtagccgag cttcgccacc tcttcgagcg gaaacatatc
     3601 gtcatgaact tttgtctcct gcaggccaat cacatccggt tggtgctttt cgacgatggc
     3661 ttcaagctgg tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa atttcatagt
     3721 cgctgccatt tattcagata aagagtgcgt ggatggtagc agaatttcgc gttgcttacc
     3781 gcctgttgtt aagtgatttg atcgatggtt gttaatgatg tcaacgatgg cgcaaaaaat
     3841 gcccgctttt ggtgcgcgct gcgtcagaat ggcgcagtaa tttccagtaa atttcgtcaa
     3901 tgatcacaat tccagaatta tattttactt ctgcataaca ttgcgttttt tattcttttt
     3961 attcacccac atcgcaaacg tattcacttt atatgcactt taaatgcata tggtttggtt
     4021 ataactcctt gatttccagt tagcctccgc cgtttatgca cttttatcac tggctgacac
     4081 gaaccctgca atctacattt acagcgcaaa cattacttat tgttaacata caaataacga
     4141 attatttact gtagaggtcg ctatgtctca gccaattacg cgtgaaaact ttgatgaatg
     4201 gatgatacct gtttacgctc cggcaccctt tataccggta cgtggcgaag gttcgcgctt
     4261 gtgggatcag caggggaaag agtatatcga cttcgcgggt agcattgcgg tgaacgcgct
     4321 gggccatgcg catccggaac tgcgtgaagc gctgaacgaa caggcgagta agttctggca
     4381 taccggcaac ggttacacca acgagccggt actgcgactg gcgaaaaaat tgatcgacgc
     4441 cacgtttgcc gatcgcgtct tcttttgtaa ctccggtgcg gaagccaacg aagcggcgct
     4501 aaaactggcg cgtaaattcg ctcacgaccg ctacggcagc cataagagtg gcatcgtggc
     4561 gttcaaaaat gcgtttcatg gtcgcacgct gtttactgtc agtgcgggtg ggcagccagc
     4621 ctattcacag gattttgcgc cactgccggc ggatattcgt catgctgcat ataacgatat
     4681 taactctgcc agcgcgctga ttgacgacgc cacctgtgcg gtgattgtcg agcccatcta
     4741 gggcgaaggc ggtgtggtgc cagccagcaa cgcgttttta caaggtctgc gtgaattgtg
     4801 tgaccgccac aatgccttgc tgatttttga tgaagtacaa accggcgtcg ggcgcactgg
     4861 tgagctgtat gcctatatgc actacggcgt gacgcccgat ctgttaacca ccgccaaagc
     4921 gctgggcggc ggtttcccgg tcggtgcgtt gttggcaacc gaagagtgcg cccgcgtgat
     4981 gaccgttggc actcatggca ccacctatgg cggtaatccg ctggcctcgg cggtagcagg
     5041 caaagtgctg gagctcatca acacaccaga gatgcttaat ggcgttaaac agcgtcacga
     5101 ctggtttgtt gagcgtctta atactattaa tcaccgctat ggtttgttca gtgaagttcg
     5161 cggcttaggt ttgttgattg gctgtgtact gaatgccgat tacgccgggc aagcgaaaca
     5221 gatctctctg gaagcggcga aagcaggcgt gatggtactg attgcgggtg gcaacgtggt
     5281 gcgttttgcg cctgcgctca atgtcagcga agaagaggtg acgaccggac tggatcgctt
     5341 tgcagctgct tgcgaacact ttgttagccg aggttcatca tgatggtcat ccgtcccgtt
     5401 gagcgctcag atgtctcggc gctgatgcag cttgccagca aaacgggcgg cggcctgacg
     5461 tcgcttcccg ccaatgaagc cacgctttcg gcgcgtatcg aaagggcaat caaaacctgg
     5521 caaggcgaac tgcctaaaag cgagcagggc tatgtgttcg tgctggaaga tagcgagaca
     5581 ggcaccgtgg cggggatttg cgctattgag gtggcggttg ggctgaacga tccctggtac
     5641 aactatcgcg tcggcacgtt ggttcacgcc tcaaaagagc tgaatgtcta taacgcattg
     5701 ccgacgctgt ttcttagtaa cgatcacacc ggcagcagcg agctgtgcac gctgtttctc
     5761 gacccggact ggcgcaaaga gggcaacggc tatttgctgt cgaaatcgcg ctttatgttt
     5821 atggcggctt ttcgcgacaa gtttaatgac aaagtggttg ctgaaatgcg cggggtgatt
     5881 gacgaacacg gctattcacc gttctggcaa agcctcggta aacgcttctt ttcgatggat
     5941 tttagccgcg ccgattttct ctgcggcacc gggcaaaagg catttattgc agaactgatg
     6001 ccgaaacatc cgatctatac ccacttttta tcccaggaag cccaggacgt catcggtcag
     6061 gtacatccgc aaaccgcgcc tgcccgcgcg gtgctggaga aagaaggttt tcgttaccgc
     6121 aactatatcg acatcttcga cggcgggcca acgcttgagt gtgacatcga ccgcgtgcgc
     6181 gccatccgta aaagtcggct ggtggaagtg gcagcaggct ggcttgccgc cgggcgtgaa
     6241 acgggtcagg cgctgagtgc gctggaggat ctcgacggtt tgctgtttac cggtagcgcc
     6301 aataccggct accagctgca tcgccagctc tccggtcagc cggagaaaat tctcgccctt
     6361 gagatgggcg gtaataatcc gctaattatc gatgaggtgg cggatatcga cgcggctgtc
     6421 catctgacca ttcagtcggc gtttgtcaca gccgggcaac gctgcacctg cgcccgccgt
     6481 ttattgctca aaagcggagc gcagggcgat gcgtttcttg ctcgtctggt tgccgtcagc
     6541 cagcgattaa cgccgggcaa ctgggatgac gaaccgcagc cgtttattgg cgggctgatt
     6601 tctgaacagg ccgcacagca ggtggttact gcctggcagc aactggaagc gatgggcgga
     6661 cgaaccctgc ttgcgccgcg cttattacaa gcagggacat cgttgctgac accagggatc
     6721 attgaaatga cgggcgttgg cggtgtgccg gatgaagagg tgttcgggcc gcttttgcgc
     6781 gtctggcgtt atgatacttt tgatgaggcg attcggatgg cgaacaacac tcgcttcgga
     6841 ctcttttgcg gtctggtttc ccccgagcgg gaaaaattcg atcaactgtt gctggaggcg
     6901 cgggcgggga ttgttaactg gaacaaaccg cttactggtg ctgccagtac cgcgccattc
     6961 ggcggcattg gtgcttccgg taaccatcgc cccagcgcct ggtatgccgc agattactgc
     7021 gcatggccta tggcgagcct ggagtcggac tcgttaacat tgcccgccac gcttaacccc
     7081 gggctggatt tttccgatga ggtggtgcga tgaacgcctg ggaagttaat ttcgacgggc
     7141 tggtagggct gacgcatcat tacgcgggcc tgtcgtttgg taatgaagcc tctacccgtc
     7201 accgttttca ggtgtctaac ccgcgactgg cggcaaaaca gggcttactg aaaatgaaag
     7261 cccttgccga tgcgggattc ccccaggccg tgatcccgcc gcacgagcgt ccgtttattc
     7321 cggtgctgcg tcagttggga ttcagtggta gcgatgagca ggtactggaa aaagttgcac
     7381 gccaggcacc gcactggctt tccagcgtca gttccgcttc gccaatgtgg gtagccaatg
     7441 cggcaacgat cgcgccatct gccgatacgc tggatggcaa agtgcatctc accgttgcca
     7501 acctgaacaa taaatttcac cgttcgctgg aagcgcccgt cactgaatcg ctgttaaaag
     7561 cgatttttaa ggacgaagag aaatttagcg tccattcggc gttgccgcag gtagcgttgc
     7621 tcggtgatga gggggcggca aaccacaatc gtctcggcgg tcattacggt gaaccgggta
     7681 tgctactttt tgtctacggg cgagaagaag gcaatgatac ccggccttcc cgttatccgg
     7741 cgcgacagac tcgcgaagcc agcgaggcgg tggcaaggct taatcaggtg aatccccaac
     7801 aggtgatttt cgcccagcaa aacccggacg ttatcgacca gagcgttttt cataatgacg
     7861 tgattgccgt gagtaaccgc caggtgctgt tttgccacca acaggcgttc gctcgccagt
     7921 cacagttact ggcaaacctg cgggcgcggg tgaacggttt tatggcgata gaagttccgg
     7981 caactcaggt ttccgtgtcg gatgcggtgt ctacctatct gtttaacagc caactgttga
     8041 gccgcgatga tggttccatg atgttggtgc tgcctca
//
LOCUS       DAJTYI010000157         8011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYI010000157 DAJTYI010000000
VERSION     DAJTYI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1056)
                     /gene="hprS"
                     /locus_tag="OO564_003509"
     CDS             complement(<1..1056)
                     /gene="hprS"
                     /locus_tag="OO564_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6654482.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRM"
     gene            complement(1056..1727)
                     /gene="hprR"
                     /locus_tag="OO564_003510"
     CDS             complement(1056..1727)
                     /gene="hprR"
                     /locus_tag="OO564_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6654483.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1860..2273
                     /gene="hiuH"
                     /locus_tag="OO564_003511"
     CDS             1860..2273
                     /gene="hiuH"
                     /locus_tag="OO564_003511"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6654484.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2382..3386
                     /gene="msrP"
                     /locus_tag="OO564_003512"
                     /pseudo
     CDS             2382..3386
                     /gene="msrP"
                     /locus_tag="OO564_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3387..4021
                     /gene="msrQ"
                     /locus_tag="OO564_003513"
                     /pseudo
     CDS             3387..4021
                     /gene="msrQ"
                     /locus_tag="OO564_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            4279..4929
                     /gene="zinT"
                     /locus_tag="OO564_003514"
     CDS             4279..4929
                     /gene="zinT"
                     /locus_tag="OO564_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6654485.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6007..6276)
                     /locus_tag="OO564_003515"
     CDS             complement(6007..6276)
                     /locus_tag="OO564_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6654486.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6333..7001
                     /locus_tag="OO564_003516"
     CDS             6333..7001
                     /locus_tag="OO564_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6654487.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7317..>8011
                     /locus_tag="OO564_003517"
     CDS             7317..>8011
                     /locus_tag="OO564_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6654488.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2318 a         1793 c         1605 g         2295 t
ORIGIN      
        1 cattcgttgc agtaaaattt tatccgcaaa gatttgttga ttgcacttga ccttaaagca
       61 aatctctttc tcgtctgaaa gagattcaag atagtccaaa agattttcaa cttccttatt
      121 gagagaaagc gaatccagtt tcaccaaaac attgtttttg tctgccctgg caagaaacag
      181 tatattctct gttaaccgcg aaatattttc cagctcttca atgtttccgg caattgtttt
      241 ttgatattca gcgatacttc tgatttgact gagcgtaacc tgattctgac ccagtaatgc
      301 attaattggc gttctaagtt catgagcgag atcgtcagca aattgactaa ggcgctcaaa
      361 atctttgact aaagcatgat gcattttatt caacgcctgc ccaagaggtt ttagttctct
      421 cggtaatgcg ttaacctcaa ccggcactcg gctatcgtta taattcagcg cttccgttac
      481 gccactcaac tttttgatct ctcgcaaccc cgtcctgatt aacaacggac ttaatactga
      541 gcacagtata atggcgacaa tgcaaattat aatgctatta attttatact gttcaagcat
      601 attatgtctg gctgaagcca atttagccac agtaaccgtt aatgatggcg aaacttcatc
      661 aatgttaatt cgtaaagcat ctatctctgt atcattaata atgcttctgt aaatgcctgc
      721 tgcgctgatt gtctcactag caggtatgtt atttaacatg tcatcactga catttgtccg
      781 gttaacaatt ttattgatgc catcactatg aatgatcaaa atatcctgac taacatccat
      841 catccggtta aagtacacag gtaacgtatc tggatttacc ccatcaatta acaactgctt
      901 gatctgcgct gtccggttaa tgagtgttgt atcatcgcgc catttcaact cacttgccag
      961 gccattatag agagtccata caattccagc gccagcaaca gactgcagaa atataaaaag
     1021 caaggttaaa cggacggtta tagataatct tttcattatt tttttaccgc tacgaatgaa
     1081 tagcccatcc cccggattgt ggcgattagc ttttcaggaa aaggatcatc aacttttgcg
     1141 cggagcctgc gaatggcgac atccaccgta ttggtatcac tatcaaagtt gattccccaa
     1201 atttcactcg caataaccgt tctgggtata atctcgccag ctctggaggc cagtagccaa
     1261 agtaactgaa actccttgcg cgtcagtgta atactgatat tgtccctgct gacactttga
     1321 ctgacagagt ccatttttaa gccactgatt tctaatgttg aattcagagc gtgatgttgc
     1381 cttaattgtg cccgaaccct tgctaacaac tcagaaaatg aaaaaggttt taccagataa
     1441 tcatttgccc cactgtccaa ccctctgact ctgtcatcga cagaatccct tgcagtaagg
     1501 caaataacag gggtttgctt tgccgttctt aacgtttgta agatctgcca gccatccata
     1561 cccggaagca taatatccag aatgatcaat gcataatcat ccttcagcgc aagataaagc
     1621 ccatctctgc catcagaaac ggcatcaatg acataacccg cttcggaaag cccctgcgtt
     1681 acccattcct gggtcctttg attatcttca ataagtagaa tcttcatgaa aataatatgc
     1741 cataaatcat atgttataga aacaacctgg ttcattacaa aattgtaatg ctgctgtaag
     1801 gttaccctgg acgttttttc gctatcctca aaactcattc acatgacaag gatataaaca
     1861 tgttaaagcg ttatttagta ctctcggtag caacggcagc attttcatta ccttctttgg
     1921 tttatgccgc acaacaaaac attcttagcg tgcacatttt gaaccagcaa acaggaaaac
     1981 ccgctgccga cgtgacagtc actcttgaaa agaaagcgga taacggctgg ttacaactta
     2041 ataccgccaa aacagataag gatggacgaa ttaaggcact gtggcccgag caaactgcga
     2101 ctactggcga ttaccgtgtc gtatttaaaa ccggggacta tttcaagaaa caaaatcttg
     2161 aaagtttctt ccctgagatt cccgttgaat ttcatattaa taaagtgaac gagcattatc
     2221 atgtgccttt gttactcagc caatatgggt attcaaccta tcgtggcagt taatgcgtaa
     2281 atatgtaaaa gctgatctct gctactgtta gggaggtgac tccctgacag attaacagta
     2341 aacagctctt gttggttaac gacaaaaaag tgtgatggct tatgaaaaag aatcaatttt
     2401 taaaagaatc agatgttacg gccgagtcgg tattctttat gaagcgtcgg caggtgttaa
     2461 aagcactggg catcagcgca gctgcatttt ctttgcctca cgctgcgcat gccgatctgc
     2521 ttagctggtt taaagggaac gatcgtccgc ccgcccccgc cggaaaaccg ctggagttca
     2581 gcaagcctgc cgcctagcaa aataacctgc cactgacgcc agcagataaa gtctccggtt
     2641 ataacaactt ctatgaattc gggctggata aagacgatcc cgcggctaat gctggtagcc
     2701 tgaaaaccga tccatggaca ctgaaaatca gcggcgaagt ggcaaaacca ttgaccctcg
     2761 atcatgatga tttaacccgt cgcttcccgc tggaagagcg tatttatcgt atgcgctgcg
     2821 tggaagcgtg gtcgatggtg gtgccgtgga ttggttttcc gctacacaaa ttgctggcgc
     2881 ttgccgaacc caccagcaat gcgaagtatg tcgctttcga aacgatttat gcgccggaac
     2941 agatgcctgg ccagcaggac cgctttatcg gcggcgggct gaaatatcct tatgtcgaag
     3001 gattgcgtct cgacgaagca atgcatccgc tcacactgat gaccgtaggt gtttatggca
     3061 aggcgttacc accgcaaaat ggcgcgccgg tgcgactgat tgtgccgtgg aaatatggct
     3121 ttaaagggat taaatcgatc gtcagtatta agctgacccg cgagcgtccg ccaaccacct
     3181 ggaatctggc agcgcctgac gaatacggtt tttacgccaa cgttaatccg catgttgatc
     3241 acccgcgctg gtcacaggct accgaacgat ttattggttc aggcggcatt ctcgatgttc
     3301 agcgccagcc aacactactg tttaatggtt acgccgacca ggtggcatcg ctgtatcgtg
     3361 gcctggattt gcgggagaat ttctaaatgc gtctgacagc aaaacaggtg atatggctga
     3421 aagtttgcct gcatcttgcc ggattgttgc cgtttctctg gctggtctgg gcgatcaatc
     3481 acggtggact gggtgccgat ccggtgaaag atattcagca ttttaccggt cgcactgcac
     3541 tgaaattttt gcttgcggcc ttgttaatca cacctctggc acgctacgca aaacagccgt
     3601 tattgatacg cactcgccgc ctgttaggct tatggtgctt tgcctgggcg acactgcatt
     3661 taaccagtta cgcattgctg gagttgggcg tgaataatct ggcgttatta ggtaaggagt
     3721 taattacccg gccttattta acgttaggaa ttatcagctg ggttattttg cttgctttag
     3781 cgttcacttc aacccagtcg atgcagcgaa aactcggcaa acactggcaa cagttgcata
     3841 acttcgtcta tcttgtcgcg atcctggccc caatacatta tctgtggtca gtgaagatta
     3901 tttcaccgca gccactcatc tacgctgggc tggctgtact gctttagcct tacggtataa
     3961 gaagttgctt tcccttttca accggttacg caagcaggcc cacaacaaat tatctctata
     4021 acttcatatt gccgacaaac ttcgcgttat cccggccgga tgcggcgcga gcgccctatc
     4081 cggcccacac agatcgcatc aagttatgtt tcgtttgtca tcaacctttc atcaccagaa
     4141 taatttattt tatttcaata agatagttca atacacctga gaataccatg ctctcatttc
     4201 ctaatgagtt gttgcatttt ttcatatgtt acaatataac attacacata atgtacatta
     4261 actctggagg aaattgtttt ggcgattcgt cttcataaac tggctgttgc tttaggtgtc
     4321 tttattgtta gcgctcctgc cttttcgcat ggtcatcatt ctcatggcaa acccttaaca
     4381 gaggtcgaac aaaaagctgc taatggtgtt tttgatgata ccaatgtaca aaaccgaacg
     4441 ctcagtgact gggatggagt ctggcaatcc gtttaccctt tactgcaaag tggcaaactt
     4501 gatcccgtct ttcagaagaa agcggatgca gataaaacca aaacatttgc tgaaattaaa
     4561 gattattatc gcaaaggtta tccgacagat atcgaaatga ttagcattga ggacggcatt
     4621 gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat atgattacga tggatacaaa
     4681 atactcacct ataaatcagg caagaaaggc gttcgttact tattcgaatg taaagatcct
     4741 gaaagcaaag cccctaaata tatacaattt agcgatcata taattgcacc acgaaaatca
     4801 tctcattttc acatttttat gggtaatgat tcccagcaat ctttattgaa tgaaatggaa
     4861 aactggccaa cgtattatcc ctatcagttg agtagcgaag aagtggtcga ggagatgatg
     4921 tctcattgat ttttttgcgc tgacttcagt tatttaacag aacagtgcct ggtagtaaca
     4981 acaataattt accaagacaa ataagtaaga caaactcaga cacgctcata cttgagtcgt
     5041 gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag gcgaacaata aatacacaac
     5101 tcagccatac agactggcag cgcaaatgtt tccaggagca agtcatctgt catggtaaag
     5161 ctaaccagaa atataggtta attttgttgt ttaaaccttt tttacttgtt agtataatac
     5221 aaaggctttc aaaaaagctg ggaaattcaa caactcttgg aagttatgga ctttgtcccg
     5281 cggctttaat ctgacggccg cgttcctttt ttggctcaat tattgacccc ttctctacgc
     5341 aacttcagtt cccgccagca actttgcggc agtttcatag gatcaatgtc taaaagaata
     5401 atggtgaccg ataagaaaac gactgaataa ctgtagattt tcgctcgaaa ccttcctgtc
     5461 agctccatag cgaatcaagc gctgaatgtc acagtatcga acagaaaaca gtgacgatct
     5521 aacccttcaa gaatattcta cgattgttct gtttaggaaa agcaaggcgg gaagtcggga
     5581 gataagtcat tgataaagtg gcggagagag ggggatttga ccccccggta gagttgcccc
     5641 tactccggtt ttcgagaccg atgatacaga gtcgaaaaat caaagagtta aaaatttttc
     5701 ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg aattacataa cattaaagaa
     5761 gaatattcca agccttcttg agtccaccac ctgttttcag gtgtcattca gacgtgattt
     5821 gccccagatt gggaaagcac gcttttttct cagcttattc cgttttgcct gtgtgttgaa
     5881 taattccact gcagttatat aaaatcggta acggctggaa atcattcaat actcgcacta
     5941 tcgaaagttt accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt
     6001 tttcgcttac attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc
     6061 aacacggttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc
     6121 cgttgcgatc tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg
     6181 gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata
     6241 ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca
     6301 taattgattt aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag
     6361 atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg
     6421 gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc
     6481 tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc
     6541 acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg
     6601 catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac
     6661 cttatcctgc ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact
     6721 gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa
     6781 ctgatgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct
     6841 tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt
     6901 ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc
     6961 aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt
     7021 cttgcttaag acgtgctacg gtttccttta cgatttgggg ttgcgacttt acctctattg
     7081 ataatgcatt ccggcagaac gttcaaatat gaacgtacga tatttaacta accgaaaaac
     7141 aaaatatagc acaggcgaga catttaccat ctaagaaaat agtatcgttt ttttacagtt
     7201 aaatcaacat cacttcctta aaatgaaaac aataaatttc aatcctgaaa cattttattt
     7261 cagcttatta ttccccagta tcacatttta tcaggatatc cttctgcgag gttattatgc
     7321 ttcctgtaaa taatcccccc ctatccactg gaaacgtctc tttttacaga actacatcaa
     7381 tcgacaatgt tcacaataat tatctctccg aatgggttga atggactaaa aacagcattt
     7441 ccggagaaaa cagggaaact gcttttaccc ggctccaatt atgtctggag aacagtgaaa
     7501 catcgttgga cttatcttgt ttaggtctca gatctctacc acgattgcct gacaatcttg
     7561 atgaaattaa tgtaagcaat aaccaactat caatgctccc cgagctacca agggcattga
     7621 aagagctgaa tgcaagcagt aatcaattat ctgcacttcc tgaattacca gtgtcgctgg
     7681 aatatataaa tgtgagtgat aaccatttgt tcgcacttcc tgaattacct gcgtcactag
     7741 aatatattaa tgtaagtgac aatcacctgt ctgtacttcc gaggttacca atgtcattgg
     7801 aattacttga tgcagccaga aatgctttgg aagtaatacc agagtttcca gaaagagatg
     7861 atcatattat aagaatattc tggcttaatc agaaccggat cacggcaatt ccggaaagca
     7921 tacttggcct cagttctgat agcgttgtca atcttagaga aaatcaacta tctcccagaa
     7981 taatgcaaac tttgttacaa caaaccgccc a
//
LOCUS       DAJTYI010000158         7991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYI010000158 DAJTYI010000000
VERSION     DAJTYI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..578
                     /locus_tag="OO564_003518"
     CDS             <1..578
                     /locus_tag="OO564_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6654489.1"
                     /translation="GSVFGVKDSHDDVVMSGAFAASLRAWSDRKALPALLWQHRMDEP
                     IGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSIGYVLKDWEYDRTK
                     EAFLLKEIDLWEVSLVTFPSNDEARISDVKNALARGEIPEQKKIERVLRDVGLSRTQA
                     KAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            593..1798
                     /locus_tag="OO564_003519"
     CDS             593..1798
                     /locus_tag="OO564_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6654490.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            1848..2048
                     /locus_tag="OO564_003520"
     CDS             1848..2048
                     /locus_tag="OO564_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654491.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            2051..2374
                     /locus_tag="OO564_003521"
     CDS             2051..2374
                     /locus_tag="OO564_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6654492.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            2371..2781
                     /locus_tag="OO564_003522"
     CDS             2371..2781
                     /locus_tag="OO564_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6654493.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            2756..3262
                     /locus_tag="OO564_003523"
     CDS             2756..3262
                     /locus_tag="OO564_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654494.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            3259..3819
                     /locus_tag="OO564_003524"
     CDS             3259..3819
                     /locus_tag="OO564_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654495.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            3828..3995
                     /locus_tag="OO564_003525"
     CDS             3828..3995
                     /locus_tag="OO564_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6654496.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            3982..5475
                     /locus_tag="OO564_003526"
     CDS             3982..5475
                     /locus_tag="OO564_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6654497.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            5475..5831
                     /locus_tag="OO564_003527"
     CDS             5475..5831
                     /locus_tag="OO564_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6654498.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            5831..6100
                     /locus_tag="OO564_003528"
     CDS             5831..6100
                     /locus_tag="OO564_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6654499.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            6242..>7991
                     /locus_tag="OO564_003529"
     CDS             6242..>7991
                     /locus_tag="OO564_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6654500.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVN"
BASE COUNT         1908 a         1980 c         2405 g         1698 t
ORIGIN      
        1 atggctccgt ctttggtgta aaggacagcc acgatgatgt ggtgatgtcc ggggcatttg
       61 ccgcttccct gcgggcgtgg agtgacagaa aagcgttacc tgcgctgctc tggcagcacc
      121 gcatggatga gcccatcggt gtttacaccg aaatgaagga agacgatgtc gggctttacg
      181 tcagggggcg gttgctcatt gatgatgatc ccctggcaaa acgcgcacat gcacacatga
      241 aggccggttc gttaaccggc ctttctattg ggtacgtcct gaaggactgg gaatacgacc
      301 ggacgaaaga agcctttctg ctgaaagaaa tcgacctctg ggaagtcagt ctggtgacgt
      361 ttccgtctaa cgacgaggcg cggatcagcg acgtcaagaa cgcgctggcc cgcggggaaa
      421 tccccgaaca gaaaaaaatc gaaagagtcc tgcgtgatgt cggactctcc cgtacccaag
      481 ccaaagcatt catggccggg ggctatatcg cactgtccct gcgcgacgct gaggatgtgg
      541 gctctgcact gaatgcactg aaaaatctga acttctaatc aggagaaata cgatggcggt
      601 agatattaaa gatgtcgaac aggtcgcgca ggagctgcag cagaagtttg acgacttcaa
      661 agcaaagaac gacaagcgcg tggatgcgat tgagcaggaa aaaggcaagc ttgccgggca
      721 ggtggaaacc ctgaacggga aactcagcga gctggaaaat ctcaaaagcg atcttgaaaa
      781 agagctgctt gagctgaaac gtccggcagg tggcgcgcaa aataaactgg ccaccgagca
      841 taaagaagcg tttgtgggct ttttgcgtaa aggccgtgaa gatggtctgc gcgatctgga
      901 gcgtaaggca ttgcaggtgg gcaccgatga agacggtggc tatgccgtgc cggaagcgct
      961 ggatcgcaac attctcaccc tgctgaaaga tgaagtggtg atgcgccagg aagccacggt
     1021 gatcaccgtt ggcggttccg actacaaaaa actggtgaat ctgggcggca cggcttccgg
     1081 atgggtgggg gaaacggata cgcgatccca gactgccacc tccagactgg agctgattga
     1141 acctctcatg ggggaaatct acggcaaccc gcaggccacc cagaaaatgc tggacgatgc
     1201 cttcttcaac gtggaggcct ggatcaacag cgagctggca accgaatttg ccgaacagga
     1261 agaaattgcc tttaccaccg gcgatggtac caagaagccg aaagggttcc tggcgtatga
     1321 atccactgat gaaaccgata aggtccgggc gttcggcaaa cttcagcata ttgtatccgg
     1381 cgaagcgacg gcggtgaccg cagacgccat tatcaaactg atttacacgc tgcgtaaggc
     1441 acaccgcacc ggcgcgaagt tcatgatgaa caacaacagc ctgtttgcca tccgtctgct
     1501 taaagacagc gagggtaact atctgtggcg tccggggctg gaactggggc agccgtcctc
     1561 tctggcgggt tacggtatcg ctgaaaacga acagatgccg gatatcgccg ctgatgcgaa
     1621 agccattgca tttggtaact tcaaacgggg ttacaccatc gttgaccgta tcggcacccg
     1681 cattctgcgt gacccgtaca ccaataaacc gtttgtcggt ttttatacca ccaagcgcac
     1741 cggcgggatg ctggtcgatt cgcaggccat caaactgctg aagattgcag cggcgtaatc
     1801 actcaggggc gcggaaccgc gccccctgtt ctgacgggtg aagaatcatg atcctgaaac
     1861 aagatctgaa atggtcaccg gacggtatgc gtgttgaggt cattcgggcc ggtgagtatg
     1921 acgacggggc gcttcctgcc cgggtgcagg agattgcact tcaggccggg ttagcagagc
     1981 gcggaaccag tgcaaaaagc agtaaagcga caaaagagaa aaaagccacg accagtaaag
     2041 agggctgagt atgcttctga caatggaaga gattaaagcc caactccggc tggatgagga
     2101 tttcgatgct gatgaccgcc atctgcaact gctggcctgt gcggcacaaa agcggacgga
     2161 aacgtatctg aaccggaagc tctatgcacc ggatgaaacc attccggaca gcgatccgga
     2221 cgggctgcac ctgccggatg atattcgtct ggggatgctg atgcttatca gccattttta
     2281 cgaaaaccgc tcgtcggtta cggaagtgga gaaacttgac atgccgcaga gttttggctg
     2341 gcttgtcggc ccgtacaggt actttccgca atgaaaattc gtcaggcgca gaccagcgca
     2401 acctacattc tgccggaccc cggtgaactg aataaacgcg tcctgatccg cctgcgggtg
     2461 gatatgcccg cggataactt tggcgtggag cctcaatacc tgattacgtt ccggacatgg
     2521 gcgaaggtta tccagaccag tgccaccacc tggcaggaaa ccgcgcagac cggggacgcc
     2581 atcacccatt acatcaccat tcgttaccgc cgggggatta ccgctgatta tgaggtggtc
     2641 tgcggtgaca gtgtgtaccg ggtgaaacgt cagcgcgatc tgaacggggc gcggcgcttt
     2701 ctgctgctgg agtgtacgga gctgggcgaa tgtgggcaga gtcacggagg caacaatgac
     2761 gacttccttt ttgcacgttg attttcagca gcccgcggag atgcgcttta accgcgcccg
     2821 tgtccggcgg gcgtttgtca cgattggtca gcgtcatatg cgtgatgccc gtcggctggt
     2881 gatgcgccgt gcgcggtcgg caccgggtga aaaccccggt tatcagaccg gacgcctggc
     2941 tcgttcgatt ggttacatgg tacccagagc cagtaaacat cgccctggtt ttatggcacg
     3001 tatagcccct aaccagcgta atggagaggg aaaccgccgt atcaccggtg atttttatcc
     3061 ggcttttttg ttctatggcg tgaggcgagg ggcaaagcgt cgtcgcagcc atcatcgtgg
     3121 tgcatccggt ggcagcggct ggcgactggc tccacgtaat aactttatgg tggaaacgct
     3181 tgaaaagaac cgcagctgga cacgctattt tctggcgcgg gaattgcgta aatcactgaa
     3241 gccggagcga cgacacagat gaaactgacg cctgttattg ctgcgctgcg tgcccgctgc
     3301 ccgtattttg aaaaccgggt ggcaggcgcg gcacagttca aaaatctgcc ggaggtcgga
     3361 aagctgagac tcccggcggc gtatgtggta ccgggtgatg actctccggg agaaaacaaa
     3421 agccagaccg actactggca ggagctgaaa gagggcttct ccgtggttgt catactgagt
     3481 aacgggcgtg atgagcgcgg tcagtttgcc tcgtatgatg tggtggacga tgtccggcaa
     3541 atgctcttta aggccctgct gggctggaac ccggaagcgt gcggtaaccc gattacctat
     3601 gacggcggca cgctgctgga tctgaatcgt catgagctga tttatcagtt cgatttttcg
     3661 gtcatcagcg agttgaccga agacgatacc cgccagcagg atgacctgaa cagtctggat
     3721 gaactgcgaa cgctggcgat tgatgttgat tatctagagc ccggtaacgg gcctgacggc
     3781 gatatcgaac atcacaccga aataaccctt ccttcctgag aatcttcatg tttgtgaaac
     3841 ctgttaaagg gcggtcagtt cctgaccctg cccgcggaga ccttttgccc gccgaggggc
     3901 gaaatgttga tgagaacaac tactggctgc gccgtgaagc agcgggtgat atccggcgcg
     3961 tgaatgaaaa ggtgaatacc gatgacgagc tttaacacca ttccgtcaaa tacgctggtt
     4021 ccgatttttt atgcggaaat ggataactcg gcggcgaata ctgcacagga cagcggagca
     4081 tcgctgctga ttggtcatgc caataacggt gcagagattg ttgccaacag tctggtgctg
     4141 atgccatcgg cagactatgc acgccagatt tgtggtgcgg gaagtcagct ggcgcgtatg
     4201 gtcgaggctt atcgccagac cgacccgttt ggtgagctgt atgtgattgc cgttccagaa
     4261 gccacaggcg cggcggcaac ggttacgctg acggtgaccg gggaagcaac cgaaagcggc
     4321 acggtgaatg tctatgtggg acgtacccgc gtgcaggctc cggtgaccaa cggcgataac
     4381 gtcacgacga ttgccagcag tatccaggat gccatcaatg ccgttccggc cctgccgttt
     4441 acggcttcat cttcggcagg cgtggtcaca ctgaccgcgc gtcataaggg gctttgcggg
     4501 aatgaaattc ctgtcagcct caattactac ggctttggtg ggggcgaagt gctgccagcg
     4561 ggcgtacaga ttgccgtggc gacgggtacc gccggaacgg gtgctccggt tctcaccggc
     4621 gcggtggctg caatggcgga tgagccgttt gattatatcg gcctgccgtt caacgacacg
     4681 gcctccgtta acacgctggt gaccgagatg aacgatacca gcggtcgctg gagctatgcg
     4741 cgtcagctgt atggtcatgt gtatacggca aagatcggca cgctgtcaga actggtgacc
     4801 gcaggtgacc agtttaacca gcagcacatt accctggcgg gatacgaaaa agagacccag
     4861 acgcctgccg acgagctggc ggcaagccgt accgcccgcg cagcggtgtt tattcgcaac
     4921 gatccggcac gtcccacgca gaccggtgag ctggtgggta tgctgcctgc gccgaagggg
     4981 aaacggttca cgatgaccga acaacagacc ctgctgtctc atggcgtggc aacggcgtat
     5041 gtcgaaagcg gggtgctgcg cattcagcgt gatgtcacca cgtacaggaa aaacgcttac
     5101 ggtgttgcgg ataacagcta cctcgacagc gagacgctgc ataccagcgc gtatgtgctg
     5161 cgcaaactga aatccgtcat taccagtaag tacgggcgtc acaagcttgc cagcgacggt
     5221 acccgctttg gtcccggtca ggcgattgtc acaccggcgg tgatcaaagg ggaactgctg
     5281 gcaacctacc gtcagcttga gcgtgcgggg atcgtggaaa actacgaact gtttaagcag
     5341 tacctggttg tggagcgtga tgccagcgat ccgaaccgcc tgaacacgct gttcccgcct
     5401 gactatgtta accagttgcg tgtctttgcc gtggttaacc agttccgtct tcagtattca
     5461 gaggagtctg cataatggcc cgtatcgggg gaacctgtta tttcaaaatt gacggtcaac
     5521 agctatcgct gaccggcggc attgaggtgc ccatgaacag gacggtcaat gatgacatca
     5581 tcggcctgga cggttcagtg gaccgcaagg aaactcaccg tgcgccttat gtcaaaggga
     5641 ccttcaaggt gccgaagaat tttccggcga gcaaaatcac ctcgtctgat gagatgacca
     5701 tcactgccga gctggcgaac ggtcaggtct atgtactgtc gtctgcctgg ctgcacggcg
     5761 aagcgaacca taatgccgaa gaaggcacgg tcgatcttga gttccacggt gaagaagggg
     5821 attaccagta atgaaagagc ttgagttaaa gaaaccgatt accgctcatg gcgagacact
     5881 ctccgtactg gagtttgatg agcccaccgg gaaagatgtc cgcgagctgg ggtatcccta
     5941 ccagatgaat caggatgagt ccgtcagact tctggcgcat gtggtatcga aatatattgt
     6001 gcggctggcg aaagtgccgc aaagctctgt cgaccagatg tctccggcag acctgaatgc
     6061 agcggcgtgg cttgtggctg gttttttcct ccaggcctga cggctgaata ccttactgat
     6121 cgcttctttg attgcgccag ctactggcgc attaatccct tcgaattgct gaatatgccg
     6181 atcagtgaaa ttcccttgct ggtcagtcag gcaaacagga tagagcagga gaaacgcaca
     6241 catggctgaa tttgagctta aggcgttgat caccggtgtc gacaggcttt ctcccgcgct
     6301 gtcgaaaatg caaaagaaaa tccggggatt taaacgccag gcggaagaag cgtcacaggg
     6361 tgggctggcg cttggtggcg gactggcagc gggtctgacg ctttccctga aatcttatgc
     6421 cgatcaggaa aacgccgcca ccgggctgaa agtcgccatg atggatgcga atggcgaggt
     6481 tggaaagagc tttcaggaca tcaataaact ggctattggc ctgggtaacc agctacccgg
     6541 tacaacggct gatttccaga acatgatgca gatgctggtg cgtcagggga tcccggcaga
     6601 aaatattctt ggcggtgtgg gtaaagcgac agcttatctt gcggtacaac tgaaaaaaac
     6661 accggaagcg gctgctgagt ttgctgcaaa gatgcaggat gctaccggaa cggcgtcaga
     6721 agacatgatg gggctgttcg acactatcca gaaggcgttt tatctgggcg ttgacgatac
     6781 caacatgttg tccttcttca ctaaaaccag ttctgttctg aagatggtga acaaggacgg
     6841 tcttcaggct gcacagagcc ttgcccccat cagcgtcatg atggatcaga tggggatgaa
     6901 cggggagtcg gcaggtaatg ccctgcgaaa agttatccag tccggattaa gcgttaagaa
     6961 aatcagggac gtcaataaaa tcatggcccg ccagaaactc ggggtacagc tcgattttac
     7021 tgacggcaaa ggaagttttg gcggtcttga taacatgttc aggcaactgg caaagctgcg
     7081 aaaactgacc gacgttaagc gaacaggtgt acttaaggca atatttggtg atgatgccga
     7141 aacccttcag gtggtcaatg cactaatcga taaaggaaag gatggctacg atcagatcca
     7201 gcagaagatg aataaacagg ccagcctgaa taaacgtgtt caggcccagc ttggtacgct
     7261 gtccaacctg tgggaggcaa tgacggggac cgcaactaac ggccttgcgg ctattggcgg
     7321 cgcattttct ggtgacgcca aaaatatcac gcaatggctg ggggagttgg gggagaaatt
     7381 cacgaagttt gcggatgaaa atccccgggt tattcgcggc gtcgtcgggc ttgctgccgg
     7441 tcttgcgatt ctgaaactgg gattgatggg cgtgggcagt gccatcagta tcgtcagcag
     7501 gatcatgtcg atgacgccga ttggcatgat tgcgacggcg attgccctgg ctgcgggatt
     7561 aattatcact aactgggatg ttgtcggacc ttattttaag aaactctggg aaaccattag
     7621 tccttatttt gaggctggct gggaacttct gaagaaggtt tttgcctggt cgccgctggg
     7681 gatggtgatc aataactggg gaccggttgt taagtggttt caggatatgt gggataagct
     7741 gaagccaatt attgagtggt ttaccgacag ttcaggtgac acggtcgatg ccattaactc
     7801 ggcgcagtgg ggcgcgggtg cttatgatgc ttatgggacg ggaataccgg cgcggggata
     7861 cataccttat ccggcggtgg atctggctca gtcaaacaac gcctccgatg ccacaggccc
     7921 gaatcccttc atgattaaca aagcttctgc gccaaaagtt gatggtgaga tcaaggtctc
     7981 ttttgtgaat t
//
LOCUS       DAJTYI010000159         7841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYI010000159 DAJTYI010000000
VERSION     DAJTYI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..450
                     /locus_tag="OO564_003530"
     CDS             <1..450
                     /locus_tag="OO564_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6654501.1"
                     /translation="NNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVE
                     RIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSV
                     NKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            560..1891
                     /locus_tag="OO564_003531"
     CDS             560..1891
                     /locus_tag="OO564_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6654502.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2215..3603
                     /gene="narU"
                     /locus_tag="OO564_003532"
                     /pseudo
     CDS             2215..3603
                     /gene="narU"
                     /locus_tag="OO564_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3685..7318
                     /gene="narZ"
                     /locus_tag="OO564_003533"
                     /pseudo
     CDS             3685..7318
                     /gene="narZ"
                     /locus_tag="OO564_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7315..>7841
                     /gene="narH"
                     /locus_tag="OO564_003534"
     CDS             7315..>7841
                     /gene="narH"
                     /locus_tag="OO564_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6654503.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENT"
BASE COUNT         2104 a         1933 c         1812 g         1992 t
ORIGIN      
        1 aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat ctccagcaat
       61 ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga agaaagagca
      121 attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct tgatttgtca
      181 tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt aaatctggaa
      241 aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa tatcaacctc
      301 agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga aaacatttat
      361 attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt ggttaatctg
      421 gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac tgaaatttct
      481 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
      541 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaagatgaa
      601 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
      661 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
      721 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
      781 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
      841 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca cctctatcaa
      901 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
      961 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat
     1021 tacacatata tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc
     1081 agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag aatcttgcga
     1141 atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg caggtaataa
     1201 aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac acaatgtctt
     1261 atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa ttaaaatatt
     1321 ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaatatat
     1381 aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg acaataataa
     1441 aataacccac attgatacta ataatacatc cgatattgga actttattcc ccataataaa
     1501 acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc ctactatgca
     1561 gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc tggcaattct
     1621 ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
     1681 ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga cagaggaagt
     1741 tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa acaatactta
     1801 ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact ttctggacag
     1861 atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat tctataagtg
     1921 tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat ctgaagggtt
     1981 gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt tagttacatt
     2041 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
     2101 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
     2161 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
     2221 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
     2281 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
     2341 ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa tctcaataaa
     2401 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
     2461 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccaatgg
     2521 ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
     2581 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
     2641 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
     2701 agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     2761 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     2821 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     2881 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     2941 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     3001 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     3061 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg tgccatcgcg
     3121 cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac
     3181 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     3241 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc gggtctggga
     3301 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggtg
     3361 aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga aacggcggca
     3421 gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcattt
     3481 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     3541 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     3601 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     3661 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     3721 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     3781 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggcgtt
     3841 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     3901 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     3961 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     4021 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg
     4081 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     4141 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     4201 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     4261 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     4321 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     4381 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgct
     4441 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     4501 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     4561 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatggggcat
     4621 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     4681 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     4741 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     4801 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     4861 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     4921 accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt ggccttcccc
     4981 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     5041 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc agtggtcagc
     5101 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc
     5161 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     5221 gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc ccataaaacg
     5281 catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     5341 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     5401 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg
     5461 gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc gtttttctac
     5521 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     5581 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     5641 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     5701 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     5761 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     5821 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     5881 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     5941 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     6001 ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc cacctggtat
     6061 gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct ttctgcggcg
     6121 gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat tgccaaagca
     6181 ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt acaaccactg
     6241 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     6301 gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     6361 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     6421 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     6481 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     6541 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     6601 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     6661 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg
     6721 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     6781 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     6841 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     6901 atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct gacgccgcac
     6961 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     7021 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
     7081 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     7141 ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
     7201 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     7261 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     7321 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
     7381 tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     7441 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     7501 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
     7561 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga tgattactac
     7621 gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa acatatccct
     7681 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     7741 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     7801 gccatgcaaa aggagatgta cgggcagttt gaaaacacct t
//
LOCUS       DAJTYI010000160         7760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYI010000160 DAJTYI010000000
VERSION     DAJTYI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..264)
                     /locus_tag="OO564_003535"
     CDS             complement(<1..264)
                     /locus_tag="OO564_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="catalyzes the formation of AMP from
                     adenosine-3',5'-bisphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6654504.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGT"
     gene            454..2397
                     /gene="cpdB"
                     /locus_tag="OO564_003536"
     CDS             454..2397
                     /gene="cpdB"
                     /locus_tag="OO564_003536"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6654505.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(2523..2903)
                     /locus_tag="OO564_003537"
     CDS             complement(2523..2903)
                     /locus_tag="OO564_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6654506.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2992..3852
                     /gene="qorB"
                     /locus_tag="OO564_003538"
     CDS             2992..3852
                     /gene="qorB"
                     /locus_tag="OO564_003538"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6654507.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            3961..4926
                     /locus_tag="OO564_003539"
     CDS             3961..4926
                     /locus_tag="OO564_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6654508.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5034..5696
                     /gene="ytfE"
                     /locus_tag="OO564_003540"
     CDS             5034..5696
                     /gene="ytfE"
                     /locus_tag="OO564_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6654509.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5741..7153)
                     /gene="cycA"
                     /locus_tag="OO564_003541"
                     /gene_synonym="dagA"
     CDS             complement(5741..7153)
                     /gene="cycA"
                     /locus_tag="OO564_003541"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6654510.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7461..>7760)
                     /gene="fklB"
                     /locus_tag="OO564_003542"
     CDS             complement(7461..>7760)
                     /gene="fklB"
                     /locus_tag="OO564_003542"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6654511.1"
                     /translation="VINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPV
                     NGVIPGWIEALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1994 a         2027 c         1971 g         1768 t
ORIGIN      
        1 agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga cttcccaacc
       61 gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta aaccgtccat
      121 gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg ctttgctgac
      181 gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg cattccgtgc
      241 aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt gttaacttat
      301 tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc atcattttca
      361 aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt ctcccttttc
      421 tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc aacgctcctg
      481 gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat catggaaacc
      541 actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc cacggaaaaa
      601 ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt gaaaaacagc
      661 gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta catgtcggcg
      721 aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac gctggattat
      781 acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct gaaaaatgcg
      841 ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag aaccaaacag
      901 ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga cggaaaaaaa
      961 cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg ctgggataaa
     1021 gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg caaatacgtg
     1081 cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc cgggctgtct
     1141 gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga aattccgggc
     1201 gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga ttttgctgat
     1261 atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt aatgccgggc
     1321 atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag cggtaaatgg
     1381 caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc
     1441 ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga tgccacacgc
     1501 cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta tctggcgctg
     1561 gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat
     1621 tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt
     1681 aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa aggccagttg
     1741 accttccgta atgccgccga tctttatctc taccccaata cgctgattgt agtgaaagcc
     1801 agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa ccagattgct
     1861 cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac ttataacttt
     1921 gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg ctatgacggc
     1981 gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt taatggcaaa
     2041 ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc ttacggcggc
     2101 aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga gaaccgctcg
     2161 gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca tccggcggca
     2221 gataacaact ggcgtttagc accgatagct gccgataaga aactggatat ccgtttcgaa
     2281 acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta tccgatgaat
     2341 aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag taagtaaaac
     2401 acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat gcgatgctgc
     2461 cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag cgcatccggc
     2521 agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag
     2581 tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc
     2641 acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc
     2701 tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg caggtcgcta
     2761 aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg
     2821 tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt
     2881 tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt actaaaagta
     2941 agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct
     3001 attactggtg ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt
     3061 cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc cctggcagca
     3121 caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac atctgcactt
     3181 cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag
     3241 catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc
     3301 ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa
     3361 atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac
     3421 tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat
     3481 ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcact cgtgattagc
     3541 gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca
     3601 cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc
     3661 gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg
     3721 ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt
     3781 agcaaattga ttggccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat
     3841 gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg
     3901 ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta
     3961 atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc
     4021 gggccgttga tcgtgccgga atacccggcg atgttacagt cgatggggcg ttatctggcg
     4081 ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg
     4141 cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc
     4201 tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc
     4261 ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga cggcaaactc
     4321 gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc gagtgtgaat
     4381 attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc
     4441 gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg
     4501 ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcacg
     4561 ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg
     4621 gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata
     4681 gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg
     4741 acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt
     4801 ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt
     4861 ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc tgtctcagaa
     4921 agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc
     4981 cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt
     5041 atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg gctctgtttc
     5101 gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac
     5161 gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga
     5221 ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct
     5281 accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact aaagtcgagc
     5341 gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc
     5401 atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac
     5461 aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag
     5521 cgggcgaact gctagaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag
     5581 cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg
     5641 atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt gagtgatcta
     5701 aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag
     5761 cccgcttctt accaataaac agccagccca accccagcgc gataaaccac agcggggtaa
     5821 ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga
     5881 acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttttcat
     5941 gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga
     6001 acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat
     6061 tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg
     6121 cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct tcctgcgcca
     6181 gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac gccgcagagg
     6241 tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct
     6301 caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa
     6361 tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca
     6421 gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga
     6481 aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc
     6541 cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca
     6601 tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg
     6661 caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca
     6721 gcactatcac cgccagcgag gcgacccagt cggagaaatc ggggaaccag aactgagcat
     6781 aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc
     6841 agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca ctaaaagatt
     6901 tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa
     6961 aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccgg
     7021 accccataaa caacccagta ccaatggcac cgccaatggc aataagctga atatgtcggt
     7081 ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta
     7141 cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt
     7201 tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg
     7261 cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat
     7321 cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa
     7381 aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg
     7441 aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg
     7501 ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata
     7561 gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc
     7621 acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg
     7681 ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg
     7741 ccttcacctt ggttgatcac
//
LOCUS       DAJTYI010000161         7753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYI010000161 DAJTYI010000000
VERSION     DAJTYI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..502
                     /locus_tag="OO564_003543"
     CDS             <1..502
                     /locus_tag="OO564_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654512.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
     gene            511..1266
                     /locus_tag="OO564_003544"
                     /pseudo
     CDS             511..1266
                     /locus_tag="OO564_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1569..2339)
                     /locus_tag="OO564_003545"
     CDS             complement(1569..2339)
                     /locus_tag="OO564_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654513.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2384..2818)
                     /locus_tag="OO564_003546"
                     /pseudo
     CDS             complement(2384..2818)
                     /locus_tag="OO564_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2830..3051)
                     /locus_tag="OO564_003547"
     CDS             complement(2830..3051)
                     /locus_tag="OO564_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654514.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(3052..3735)
                     /locus_tag="OO564_003548"
     CDS             complement(3052..3735)
                     /locus_tag="OO564_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6654515.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3799..4110)
                     /locus_tag="OO564_003549"
     CDS             complement(3799..4110)
                     /locus_tag="OO564_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654516.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(4107..5009)
                     /locus_tag="OO564_003550"
     CDS             complement(4107..5009)
                     /locus_tag="OO564_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6654517.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            5449..6408
                     /locus_tag="OO564_003551"
     CDS             5449..6408
                     /locus_tag="OO564_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6654518.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6408..6803
                     /locus_tag="OO564_003552"
     CDS             6408..6803
                     /locus_tag="OO564_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6654519.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6986..7366
                     /locus_tag="OO564_003553"
     CDS             6986..7366
                     /locus_tag="OO564_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6654520.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            7412..7753
                     /locus_tag="OO564_003554"
                     /pseudo
     CDS             7412..7753
                     /locus_tag="OO564_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT         2108 a         1980 c         1917 g         1748 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg ctacgtactt ctcggggcgc ttacgttgat
      541 gcagaagcca tatgtgaagc ggcttcacga ccatcaatgc gttttgtcca gccaagaact
      601 gagacccagc aagcaataag ggcattacac cgggtcagag aatcgctcat cagagataag
      661 gtgaaaacaa ccaaccagat ccacggtttc ctgctggaat ttggaatcag cctgccgaca
      721 ggggatgccg taataaaacg gttgtctctc gtattggctg aacacgaaat ccccgaatat
      781 ctgagccgcc ttttggtgag gttgcatacc cactaccttt accttgttga gcaaatagca
      841 gagctggaat cagaattaag tcagtcaatc aacgctgatg ataccgctca gcgcattatg
      901 acaattccta gtgttgggcc gattactgcc agcttactat cgtcgcaact tggtgacggg
      961 aaacagtttt catgcagcag agattttgcg gcttccacag ggctcgttcc ccggcagtat
     1021 agcactggag gaaaatcgac tctgttgggt atcagtaagc gaggtgataa aaatttacgc
     1081 cggttactgg ttcagtgtgc ccgctcattc atgatgcagc ttgaacgcca gcatggaaaa
     1141 cttgctgagt gggttcggga gcaactcaac aagaagcatt cgaatgtggt tgcctgcgct
     1201 ctggcaaata aactggcccg aattgcctgg gcaatcacta ctcagcaaaa agaatatcag
     1261 gcctgatata tagatgctga caaaactcaa ttagcttatt gcaaacaata agatacaagt
     1321 taccgtctgg ttttgcgaag attgattatt gatgacatga acggcaaatc gacctgatta
     1381 aaaacctaac aggggaaaat ggctcactga agccgagggg atttttagga ttatcaggtg
     1441 cggaactcat cgaggcgcgg gcatcccttg cccacagaga cgccggatag atttaagcaa
     1501 gcccaccatt aatcaaaaat cagtgttgca aaaacggggg tgaccataga ttttccccgc
     1561 catcgtcatc atttccggat ttttgccagg gcatccgcca tgctgcggat ttcggcccgg
     1621 gcttcccgga caacttcccg cagaacacca cggtcaaacc accggcgggg cggttcatcc
     1681 ggtatacgca gcacatcgca ggccagccca ctggacagga tatgctcaat acaatcgctg
     1741 accagataca tttccaccgc cgaaaaacgt gcgccccggt tcaggatggc atcgagcacc
     1801 acgggcaggt tttcatcgtc ttcatcgagc cagcaggggt gatcgctttc cttcacgacc
     1861 aggcgaaagc ggcgcgtctg gcgggtcagc aacgtctgac cgcttgtggt cagcgccgcc
     1921 cggatacgcc gcgcatcttc tgccccccgg caactgacac tgcatactgc atcgcgcacc
     1981 gcatcaagaa ctgccctgat ttcgtcttcg gtcagccatt cacaggcata cgggaaagac
     2041 acctgcgttc cgtccgccgg aagtagcggc agcgactgcc actgactgcc ggacagaaca
     2101 agttccgctc cggttccggt cagtttctga atggcggagg ccaggtgcgg cggcagcacg
     2161 ccatcgtggc ggttaacgag taacgtcgcc agccggggcg catccagccg cacgtaaatg
     2221 ctgatccagc ccggatagtt cgggttacat tggtcaacat ccgtctcttc cggccagccc
     2281 tgcgccagca gcaggttacg ggcgtgacga caaatcagag cattcagcgt ttcattcata
     2341 attcatgaac tccctgtatc gccgcccgca ggcggcgata acatcagtcg ttttcgtgtg
     2401 tgttgttttt cagcaggctg cgggcctgat caaccatcgc ctgtcggcgg gtaacgtatt
     2461 ccgggagggg gaaaccgtga tcccgggcag cccattcacg tttccacagt gcctgttcag
     2521 cgatctgagt cagggtctcc agcagttcag cagggacggt cacctggcgg ttttcgcgca
     2581 cgttgctgat aatctccatc gccgcggcgg aagaaatacc ggactcaatc cgctagtctt
     2641 ccgggatgcc ctccaggtgc gccaggacgg tcctgatttc atgatcggac agggaaatat
     2701 ccattccgtc agcgagggcc tgaatctctt ccggggacca gataaccagc atcagcggcg
     2761 tgtctcccga atactttgct gccagcgccc ggcatagcgt ttcgcatgtt ccgtacatac
     2821 tctctcccct caatctttga agcaggaatc cagttccaga atttcggtat atgcctccat
     2881 caagaggcgg tttgcgcgaa acaacgtctg acgcaccaga cgttccggca gggtgtcagc
     2941 attaaagctg taccgccgct ccatctcatt catggttgta cgcaggtcgc acatggcatt
     3001 ggccacctcc gccacttcgg cgcgaagttt ttcgtgtccg gcatagttca tttacgcagc
     3061 ctccggcata aaccagtcat cattcgcggc cccctgctgc atggcccgct gtacggcagc
     3121 cagtcgttgc tgtccggcac ggtgatactg ctcaagcagc tcgataccga tataccggcg
     3181 tccggactgg agggcggcga cgcaggttga accgctgcct gcaaacgggt ccagcactat
     3241 cgcattcggg tgtgtgaagc tctcaatcag cggttgcagg ctggtaacgg gcttttccgt
     3301 cgggtgatgg cggttgcccg aatatttcca gcccaacacg tccggcagcg ggttttgtgg
     3361 cagacgtgga cggccttttg ccaggatgta ggcgcattcg tggcgatagc ccacatatgc
     3421 ggccttcgat gtgtagtttt tggtgaagac caggtgacca acaacgctga atcccgcatt
     3481 tttccaggca gacataaagc gatcgacgcg gttccagcca tagaagctca ccatcagcgc
     3541 gtcttttttg agtacgcggt acatttcatt acaggccggt tgcagccatt catcagtttt
     3601 atcaccagca atggtacgcc cctgacggtc acgaaaaccg acgagatatg gcggatcggt
     3661 gaggatgaaa tcaatggcgt tatcaggaat acgggccata acatcgatgc agttaccgag
     3721 gacaaaacgg gacataacag atccttctct tacagagggc acccctgtgg tgcccgttcc
     3781 ccgctgcctg tgggctgttc atcgcgtccg gtaacgggtc aggccgaccg tggaataccg
     3841 cagccgtcgt ctttttgaca gcaaggttat gccgcgaaag cggcttgaag ggcgttcatc
     3901 agaaaagggc gacgggctgg cgtacactgt caccgacgcc agccgggagc ggtaaaagcg
     3961 cccgtcccgt gcccgccgga cgcgggacac tgaccacagg cagcgtttat gccgcgccgc
     4021 gtcagccggc gcagtaaaaa gaggaacacg gcgacatcct ttgtcggaaa agaaacgggc
     4081 ggttgcccgc ccgtctccgt catacttcat ccgccataat gcgccacatt gtcgactatc
     4141 cacgcaggtc cggtgacttc cggctgctca tcgtcatctg tcggggaaga ccattcaagt
     4201 tccccccaca gcacatcgac gagctctcca cgctcataca actgccagcc acagaaatca
     4261 cagccctgtt cggcatacca gtgctccagc gtgcagctga aacggcggct taattctgca
     4321 ataacgtcgc tttccggctg acaccacggc gtgtcgaaat ccacctgaat gaagttttct
     4381 ccctgactgc tgatattcag gtcatatcca caaggccact tcacgccata ccgttccgta
     4441 taccagtgat atgcagaagg aacaccgttc agaacaccac cattaaagcc gttcacttca
     4501 acatccaggc gggtcggcag aaccgtcagc aggtcaaacg gcatggtttt ttccggcact
     4561 acgttgtcac acagacggtt ccaccacaca gatacatcct cgttacccca tatgtcacac
     4621 cagtcacctc tttttgcggt gaaatgcaca gtgatggttt ccctggcatc atccggtaat
     4681 ccttcccagc gacgctggcc agtaccgctc tgtaaccaga gttcatgcag catcaggcag
     4741 ttttgttcat ccagcaacac accgttctgc aggtgtgtca gccagcgggt gaacgcgata
     4801 ttttccgtcg atacagcacc acgtccggca gccgtcactc cggggcactg ttcagagcgg
     4861 atattttcag tgatctgcag aagtccggca cttccggcca gaaacagctg aataccttca
     4921 tttgtggcgc ggcgataaag cggtgtgact gcaccgctgg ccagtcgttt aatctcagcg
     4981 atctgtgccg gttcaccagg aaaatacata cgattcgagc accagtttgg catgggaatt
     5041 ctcctctcac atgcggcctc catcaggccg tgccgcacct gtaaccgggc gaccgcatac
     5101 gaatgcaggt gcggccccga agccgcagcg cgtggaggag gaatgacgaa acgtgaccgg
     5161 gtgaggaggc cggcgacagc gacacacttg catcggatgc agcccggtta acgtgccggc
     5221 acggcctggt taagtagtat ccgtctcagt tcgtggcgca aaatgagaaa ccaataagaa
     5281 ctcaaaatga accacaacaa gaacataaag agactctaaa agagcaacac cgcttgacat
     5341 agactcaaaa tgagtcacaa taagaaacaa taaaaaactc aaaaagatgc accgatataa
     5401 aggatgctca atttgagata aacacaaact caaaacgagg caaaaagaat gctaaaagta
     5461 tcatgcgatg atggttcaac taacgtaaaa ctggcctggc ttgaagatgg cgaggtaaga
     5521 accagtctgt caggaaacag ctttaaagaa ggctggaacc cgggactgtt taatgccggc
     5581 aaagtttaca attacgtcgt ggatgaaaaa aaatacacct atgatttggg aagcactgct
     5641 gtcataggta caacacatgt cagttatcaa tacagcacca ccaatctgct tgccatacat
     5701 catgcactgc tgacatccgg ccttcagcca caggatgtag aactgacagt aacattacct
     5761 gtgaccgaat tctttgataa tgacaatcaa ccaaatgaag aaagaataga aagaaaaaag
     5821 gcaaatgttc tccgtgagat atctctgaat aaaggggaaa catttaaaat taaaaaagtt
     5881 aatgtaatgc ctgaatcttt gccagctgca tttgaatcac taaaaaaaga taaagtaaac
     5941 aaacttgaac gcagcctgat tatcgatctt ggaggaacca ctctggattg tggtttaatt
     6001 ctcggtgcat ttgaaggcat ttctgaaatc cgtggctatt cagaaatcgg gacatcccgc
     6061 atcactcata ccgtcatgaa tgcgctgacg aaagcctcca caccatgtaa ttattttatt
     6121 gctgatgagt taataaaaaa cagacacgat aatgaatatc tccagacatt aataaatgat
     6181 gtagctgaaa taaaaaacat ttctcatgta atagacagag aagtgaaatc tctggcagaa
     6241 agcatcaggc aggaaatttc aacattctca ggaatgaacc gaatctatct cactggcgga
     6301 ggagccgagc ttatttaccc tcacataaag caatatttcc cgaatctgaa agtgaacaag
     6361 gtggatgaac ctcagtttgc attggtgaaa gcaatggtgc atgcgtaatg gaaagcagtg
     6421 atccaaaaaa aagaaagaaa gttgtggcat acctgcaccc ggcactttat ccgcaggaca
     6481 acctgacaca acaaacaata gactctctcc cagtacaaat gcggggagat ttctacagac
     6541 aatcccttat ttgcggagca gctctttatt ctgttgaccc ccgactgtta acgctaatca
     6601 gcggtttttt cagtgagaaa atcactgcag aaaatctggt gaaacttata gaacaaacaa
     6661 caggatacac atccacatca attgatataa gtgtgttaaa aaacatcata gaggcatctt
     6721 cagaaaacaa atcagagagc atcacttcaa aagatgattt cgaagaacaa accagacgca
     6781 atctttctat gttaaaaaaa taataaaaga agagcaggtt cagtcaatta cctgctcttc
     6841 ttttatacat actaaaaaat cagattttta cgaatattca gattatcata aaaaatgcaa
     6901 cagacaggaa tgcaataatt ttgcattatc tcgttgcttg taatgcaaat atgcattatt
     6961 gtcagtgtgt gacaataaca gagagatgac gatggcgacc attaacgcac gtattgatga
     7021 tgatataaat aaccaagcag atgaagtgct gaagctgatg aatatcagtc aaactcaggc
     7081 tattgcagct ttttatcagt atattactga acaaaagaaa ctgccatttg tgatcacctc
     7141 aattgtaaaa acgccgcatg atttattacg tgaaagtaca gacatgttag ctgaagctct
     7201 tgcagtgatt tccaatctac aggtgtggac tgaacagcag gatgggattg gaaaagcaaa
     7261 gctaatggag tattaccgcc gcctcgacgc actgtactgt tgtgcaaaag agaaaatagg
     7321 tcttctttca gataaccggg atgctgaact aggctgtgtc ccttaatcat ctgagttata
     7381 gtaactgtct gtttacacaa catattgaac tatggctcgc tacgaccttc ccgatgaggc
     7441 atggaccatc atcaagcctc tgttacctcc tgaacccgcc acaccacggg ccggacgccc
     7501 atgggctgag caccgtaaaa tcatcaatgg catgttctgg gtgttatgtt ccggtgcacc
     7561 atggcgcgat ttaccctaac gatatggggc atggaaaact gtttataacc gctttaaccg
     7621 atggtcaaag tcaggcgtga ttaatattat tttcaacagg ttgctttcgc tacttgatgc
     7681 aaacggcttt attgactggt ctgccaccgc gctggatggc agtaatatcc gggcgctgaa
     7741 atgtgccgcc ggt
//
LOCUS       DAJTYI010000162         7738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYI010000162 DAJTYI010000000
VERSION     DAJTYI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..554
                     /locus_tag="OO564_003555"
                     /pseudo
     CDS             1..554
                     /locus_tag="OO564_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            555..1658
                     /locus_tag="OO564_003556"
                     /pseudo
     CDS             555..1658
                     /locus_tag="OO564_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            1703..2026
                     /locus_tag="OO564_003557"
     CDS             1703..2026
                     /locus_tag="OO564_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6654521.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            2023..2439
                     /locus_tag="OO564_003558"
     CDS             2023..2439
                     /locus_tag="OO564_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6654522.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            2405..2929
                     /locus_tag="OO564_003559"
     CDS             2405..2929
                     /locus_tag="OO564_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654523.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            2926..3489
                     /locus_tag="OO564_003560"
     CDS             2926..3489
                     /locus_tag="OO564_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654524.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            3499..3660
                     /locus_tag="OO564_003561"
     CDS             3499..3660
                     /locus_tag="OO564_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6654525.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            3657..5153
                     /locus_tag="OO564_003562"
     CDS             3657..5153
                     /locus_tag="OO564_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6654526.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            5153..5509
                     /locus_tag="OO564_003563"
     CDS             5153..5509
                     /locus_tag="OO564_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6654527.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            5509..5845
                     /locus_tag="OO564_003564"
                     /pseudo
     CDS             5509..5845
                     /locus_tag="OO564_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            5930..>7738
                     /locus_tag="OO564_003565"
     CDS             5930..>7738
                     /locus_tag="OO564_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6654528.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLA"
BASE COUNT         1918 a         1771 c         2310 g         1739 t
ORIGIN      
        1 atggctccgt ctttggtgta aaggacagcc acgatgatgt ggtgatgtcc ggggcatttg
       61 ctgcttccct gcgggcgtgg agtgacagaa aagcgttacc tgcgctgctc tggcagcacc
      121 gcatggatga acccatcggt gtttacaccg aaatgaagga agacgatgtc gggctttacg
      181 tcaggggacg gttgcttatt gatgatgatc ccctcgcaaa acgtgcacat gcacacatga
      241 aggccggttc gttaaccggc ctttctattg ggtacgtcct gaaagactgg gaatacgacc
      301 ggagcaaaga agcctttctg ctgaaagaaa tcgacctctg ggaagtcagt ctggtgacgt
      361 tcccgtctaa cgacgaggcg cggatcagcg acgtcaagaa cgcactggcc cgcggggaaa
      421 tccccgaaca gaaaaaaatc gaaagagtcc tgcgtgatgt cggactctcc cgtacccagg
      481 ccaaagcatt catggccggg ggctatggcg cactgtccct gcgcgacgct gaggatgtgg
      541 gctctgcact gaacggagaa gtggaaaccc tcaacggaaa actgactgaa ctggaaaatc
      601 tcaaaagcga tcttgaggct gagctggctg aagttaagcg tccggcaggt ggtacgcaaa
      661 ataaagttgc cggggaacat aaagaagcgt ttatcggttt tatgcgcaag gggcgtgaag
      721 atggcctgcg tgagcttgag cgaaaggcac tgcaggtggg taatgatgaa gatggtggtt
      781 atgccattcc ggaagaactg gatcgcacca ttctgacgtt gctgaaagat gaagtggtga
      841 tgcgccagga ggccaccgta atcactctga gtggctcgga ttataaaaaa ctggtgaatc
      901 tgggcggcac gacgtccgga tgggtggggg aaacggaagc gcgtccggaa accgccacct
      961 caaaactggg actgattgag ccctttatgg gggaaatcta cggcaacccg caggccacac
     1021 agaaaatgct cgatgatgct ttctttaatg tggaagactg gatcaacagt gagctggcgc
     1081 tggaatttgc cgaacaggaa gaaattgcct ttaccagtgg cgacggtagc aaaaaaccaa
     1141 aaggttttct ggcttatgag tccaccgatg aagatgacaa gacccgtgcg tttggcaaac
     1201 ttcagcacat tgcttccggc gcggcttccg gcgtgaccgc tgatgcgatc attaaactga
     1261 tttacacctt gcgtaaggcg caccgcagcg gcgcgaagtt catgatgaac aacagcagcc
     1321 tgtttgccat tcgtctgctg aaggataacg acggaaatta tttgtggcgt ccgggtattg
     1381 agctgggtca gccttcttct ctggcagggt atggcatcgt tgagaatgag cagatgccgg
     1441 atattgccgc cgatgcaaaa gccattgcgt ttgggaactt caaacgcggc tataccatcg
     1501 ttgatcgcat cggtacccgt atcctgcgtg acccgtacac caacaaaccg ttcgtgggtt
     1561 tttataccac caagcgaacc ggcggtatgc tggtggattc tcaggcgatt aagctgatga
     1621 aaattggggc tgcaacccgc cagaaagccg ctgcgtaatg cggttttttg tgcccgcgta
     1681 atcgcgggca caggaggaaa atatgctcct gaaagaagag gagattaaat ctcatctccg
     1741 gctcgatgat ggtttgtaca gtgacggcga ttttctgaaa ctgctggcac aggcggtaca
     1801 gaaaaaaaca gagacatacc tgaacaggaa gttgtatgca ccggaagaga cgattccgga
     1861 agatgatcct gacgggatgc atctgactga tgacgttcgt ctggcaatgc tgatgctggt
     1921 cagtcatttt tatgaaaatc gctcaacgat caccgatgtg gagaaactgg aaacgccaat
     1981 gagtttcaga tggcttgctg gcccttacag gattgtcccg ctatgaaaat caggcaaagt
     2041 cagaccagtg ccacttacct tttaccggat cccggagagc tgaatcgccg gataaagatc
     2101 cgccttcgtg tggatgaacc caccgctgat tttggtacgg agccaacgta tccggagtcg
     2161 tttgatgtct gggcaaaggt ggctcagcca ggcgctgccg cttatcaggg ctcagtgcag
     2221 acagaaaata tcgttacgca ttattttacg atccgtttcc ggcacgacat cacggcagat
     2281 cacgaagtgg tttattacgg ccaagagtac cggatccggc gaatacgtga cctgaacggt
     2341 cagcggcgtt ttttattact ggaatgtgaa gaactgcgta cagcgcgacg acggggtgaa
     2401 tgccatgaat cagacagcat ttttacacgt cgactttaaa caaccagagg agctggagtt
     2461 taatcgcgcc cgtctgcgtc gggcgtttgt gcaaatcggg cgcgtttaca tgcgtgatgc
     2521 cagacgtctg gtgattaaac gcggacgctc cggtccggga gagaacccgg gatatcagac
     2581 cgggcgtctg gcccgctcca ttggttatta cgttcccaaa aaaacgacgc gtcgccccgg
     2641 actgatggtg aaaatttccc ctgaccagaa aaacgggcag gggaatcgcc gttttcctga
     2701 aggtgctcct tattatccgg cgtttctgta ttacggcgtt cgccattcag cgtatggaat
     2761 ggataaaaag gataagcggc agaagaagca ccattcttcg actttccggc tggccccgcg
     2821 taataacttt atggctgacg ttattgagcg tcagcgtcac tggacgcagg aactgttgtc
     2881 cagtgagctg cagcgttcgt tacgtccggt aaaaaggaag cataaatgaa actgacaacg
     2941 ataattgcgg cgctgcgtga gcgatgcccg cgttttgagg agcgtgttgg tggcgcggcg
     3001 cagtttaagg cgatccctga tgccggaaaa cttcgcctgc ctgctgccta tgtggttccc
     3061 tctgacgatg cgccggggga acaaaaatca cagaccgatt actggcagga tttgactgaa
     3121 ggcttctccg tgattgtggt gctcagcaat gagcgtgatg aaaaagggca gtgggcagcc
     3181 tatgacgccg ttcatgatgt ccggagagaa ctctggaaag ccctgcttgg atggatgccg
     3241 gacccgcaag ggggcgagat tgtttatgtc ggtggcaccc tgctggatct gaaccgttac
     3301 gaactgtatt accagtttga ttttacggcg aagtatgaaa tcacggaaga agacacgcga
     3361 caggcagagg acgtgaatgc cctgccggat ttatccctgc tgagtattga tgtggattac
     3421 atcgatcctg gtactggccc ggatggtgac attgagcacc atctggaaat gcgtttccct
     3481 cagaattaag agtccctcat gtttgtgaaa cccctgaaag ggcggtcggt tcctgaccct
     3541 gcccgtggag accttttgcc ttccgaaggg cgaaatgtgg aagaaagcag ctactggttc
     3601 cgccgtatag cggcgggtga tgtggtacgt gttaaacagg ataaggctaa aaaatcatga
     3661 cgataagttt taatactgtt ccgtcgaata cgctggtgcc gttgttctac gccgagatga
     3721 acaattctgc ggcaaataca gcggtgacca gcgcgcctgc attactgatc gggcatgcca
     3781 gcaacgatgc agccattgag gttaacagcc tggtgctgat gccatcggca gattatgccc
     3841 gccagatttg tggggcgggg agccagctgg cgcgtatggt ggaagtctac cgtcagacag
     3901 atcctttcgg tgaactgtat gttattgcag taccggaagc cagaggggcg gcggcgacgg
     3961 tcagggtgac ggttgccgga gaagcagagg aaagcggcac cctgagtctg tatgtcgggc
     4021 gctcccgtgt acaggtgcct gtggtgaatg gcgatgatgc cactgcggtt gccaccgcga
     4081 ttaaggaagc ggtaaacggg gttatcaccc tgccgtttac ggcgtcatct gatgcaggcg
     4141 tggtgacgct gactgcccgt cataaggggc tgtatggtaa tgagttaccg gtctgtctga
     4201 attattatgg ttctggtggt ggtgagattc tgccagcagg gcttcgggtg gtgacggaag
     4261 tcggtgctgc cggtagcgga gcgcctgatc ttaccgccgc tgttgctgct atgggcgatg
     4321 aggcattcga ctttatcggt ctgccgttca acgatgccgc ctccatcaat atgatgatga
     4381 ccgaaatgaa tgacagcagc ggtcgctgga gttatgcgcg ccagttatac gggcatgtct
     4441 ataccgcaaa actgggaacg ctgtcagagc tggttggtgc cggagatatg cataatcagc
     4501 aacatatcac gcttgccggt tacgaaaaag aaacccagtc gcctgtcgat gaactggttg
     4561 ccagtcgcct tgcccgtgaa gcggtattta tccggaatga tcctgcccgt ccgacacaga
     4621 cgggggagct ggtggggatg cttccggcac cgaaaggtaa gcgattcatc atgacagagc
     4681 agcagaccct tttatctcac ggcgtggcga cggcttatgt ggaaggcggc acgttgcaga
     4741 tccagcgttc cgtaaccacc tacaaaaaga atgcgtatgg cgtggcagac aacagctatc
     4801 tggacagtga aactctgcat accagtgcac atgttcttcg caaactgaaa tcggtcatca
     4861 caagcaagta cggacgtcac aagctggcaa atgatggtac ccgttttggt ccggggcagg
     4921 cgattgtcac tcctgccgtt atcaaagggg aacttctggc gacatatcgt cagatggagc
     4981 gtgccggtat tgtggaaaat tacgatctgt ttaaacagta tctgatagtt gagcgtgatg
     5041 cagataaccc gaaccgactg aatacgctgt tcccgccgga ttatgttaat cagttacgtg
     5101 tctttgcggt ggttaaccag ttccgtcttc agtattcaga ggagtcagca taatggcaaa
     5161 gatcgccgga acctgttttt ttaaagtgga tggtcagcag ttatcgctga caggtggcat
     5221 tgaagtgccg atgaacacca atgtccggga tgacatcgtc ggcatggcag gggatgtgga
     5281 ttacaaggag acctggcggt caccttacgt taagggcacg tttaaagtgc ccaaaaactt
     5341 tccggtcgac aaaattacca cctcagacca gatgacgatt accgctgagc tggcaaacgg
     5401 catggtgtat gtgctttcgg cggcatggct gcacggagag gctaatcata atgccgaaga
     5461 aggcacggca gatcttgaat tccacggcga agagggaggg tatcagtaat ggcaatgaac
     5521 gttacagaaa tagttttaaa aaaaccggtg acagcgcata acgaaatgct gcatgtgctg
     5581 gagttgcgcg agcccacgta tgacgaaatc gaggcgctgg gttttccttt cattatttcc
     5641 ggtgaaggca gtattaaact ggacagccag gtggcactga aatatatccc gttgctggcg
     5701 gggatcccgc gttcatcggc ggcgcagatg gcaaaactgg atatttttaa gaccagcatg
     5761 cagatcctgc gtttttttac ccagtcggag acgggaagca cctccgggaa acgactctac
     5821 aatgttgcct ggttctggaa actgaatcca ctggagctgc gacgggtggc tgtttcgcag
     5881 tttacagaac tggaagccga ggccgtccgc attaacgagg agatgaagca tggctgacag
     5941 ttttcagctg aaggcgatca tcactgccgt ggataaggta tcggcaccgc tgaagggaat
     6001 gcagcgccag ctgaaaggtt ttaaaaagga gtttgccagc ctgtcactgg gcgcagcggg
     6061 tgccggaacc gcagtactgg gggcgctggc gctcccggtc aaatctgcca ttgcccttga
     6121 atcaaaaatg gcggatgtcc ggaaagtggt ggatggtctg gatacgccgg aagcgtttaa
     6181 ggcaatgacg gagcaggtgc gcgacctgtc aacagaactg cccatgtcgg cggaaggtat
     6241 cgccgaaatt gtggcggcgg gtggtcaggc tggtatcgcc cgtgacgagc tgatgcagtt
     6301 tactgacgat gccgtgaaga tgggcgtggc ctttgacgcc acggcagaag aatccggtca
     6361 gatgatggcg cagtggcgca ctgcctttaa actgacacag ggagaggtgg cagggcttgc
     6421 ggataagatt aactatcttg gcaataccgg tcctgcaagt gcgaaaaaga tttctgatgt
     6481 tgtgacccgt attggccctt taggcagtgt tgcgggtgtg gcctccggag agattgccgc
     6541 aatgggggca accattgccg gaatgggggt ggagtcagaa attgctgcga cggggataaa
     6601 gaattttatg ttgtcgctga cagcgaggga ttctgccacc aaatcgcaga aaaaagtgct
     6661 ccgctcgttg cgcattagcc cgaaaaaact ggcggcggat atgcagaaag acgcccgcgg
     6721 ggccatgctg cacgtactgg attctctggc aaaagtgccg aaagaaaaac aggctgctgt
     6781 gctgaaagcg ctgttcggga aggaatccct gggggccatt gcgccgctgc tcacgaatct
     6841 ggatttgttg cgaaccaact ttaatcgtgt ggcggatgcg cagcagtatg gcggctcaat
     6901 gcagaaagaa tatgccgccc gtgccgcgac gacagaaaat cagttgttgc tgctgcagaa
     6961 ccagatcaat gcgatttctt ccacgctggg ggaaaccttc ctgccttcac tcaatgaagg
     7021 cataaaagag atgaagcctt ttctggaaga agtgcgcacg tttgtcagag aaaatcctga
     7081 ggtcgtaaaa accatcgcga aaactggtgc ggcattactg acgatgggcg tggcgattgg
     7141 cacattgaca cgcataacaa aaatcatggg tagcgtgatg aatatgacgc cggcaaaggg
     7201 attaattgcg cttctcgtcg gtggtgcata tctcataatt gataactggg aaacggtcgg
     7261 tccggttgtg aaaaaagtct ggcaggaggt tgatcaggtt gtcagagcaa tggggggatg
     7321 ggaacaggca gtaaaaacaa tagcaacggt ttctgccctg tatattggtg tgaaggcggt
     7381 ggcaagtatt cgtgcggcaa cagtcgccca gaatcaatgg acaacggcgg caggaaaaac
     7441 agggctgaaa ttaaagggcc tcggaaagat cagtctgatt ggtggattgc ttgaactggg
     7501 catgatggcg caggagtttg aaaaagaaca tccctggctt gttaaaaatt ttgtggctga
     7561 tgcactgaac agtggatttg gtctgaatga taagttcgac gagtggggca aacagttcca
     7621 tgattttgtc tatgacatga ccggatggca gatgccacgt ggtgatgggt atttatctcc
     7681 cgataaacgc tacaccccga atgtatcact ggaaaggaat cagctgttaa gccttgca
//
LOCUS       DAJTYI010000163         7614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYI010000163 DAJTYI010000000
VERSION     DAJTYI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            194..1168
                     /gene="hemB"
                     /locus_tag="OO564_003566"
     CDS             194..1168
                     /gene="hemB"
                     /locus_tag="OO564_003566"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6654529.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1274..2125)
                     /gene="tauD"
                     /locus_tag="OO564_003567"
                     /pseudo
     CDS             complement(1274..2125)
                     /gene="tauD"
                     /locus_tag="OO564_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2110..2949)
                     /gene="tauC"
                     /locus_tag="OO564_003568"
     CDS             complement(2110..2949)
                     /gene="tauC"
                     /locus_tag="OO564_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6654530.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(2946..3713)
                     /gene="tauB"
                     /locus_tag="OO564_003569"
     CDS             complement(2946..3713)
                     /gene="tauB"
                     /locus_tag="OO564_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6654531.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3726..4652)
                     /gene="tauA"
                     /locus_tag="OO564_003570"
     CDS             complement(3726..4652)
                     /gene="tauA"
                     /locus_tag="OO564_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6654532.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(4919..5434)
                     /locus_tag="OO564_003571"
     CDS             complement(4919..5434)
                     /locus_tag="OO564_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654533.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            5627..5878
                     /locus_tag="OO564_003572"
     CDS             5627..5878
                     /locus_tag="OO564_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6654534.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            5880..>7614
                     /locus_tag="OO564_003573"
     CDS             5880..>7614
                     /locus_tag="OO564_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654535.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIH"
BASE COUNT         1843 a         2037 c         1949 g         1785 t
ORIGIN      
        1 ataataacca agagctataa ataaagatta aaaatatttt cagtgaatat gaaattattg
       61 ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc aacatcgcga
      121 caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta attccacaaa
      181 catcaggcag accatgacag acttaatcca acgccctcgt cgcctgcgca aatctcctgc
      241 gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt tgccgatctt
      301 tgttgaagaa gaaattgacg actacaaagc cgttgaagcc atgccaggcg tgatgcgcat
      361 tccagagaaa catctggcac gcgaaattga acgcatcgcc aacgccggta tccgttccgt
      421 aatgactttt ggcatctctc accataccga tgaaaccggc agcgacgcct ggcgggaaga
      481 tggactggtg gcgcgtatgt cgcgcatctg caagcagact gtgccagaaa tgatcgtcat
      541 gtcagacact tgtttctgtg aatacacttc tcacggtcac tgcggtgtgc tgtgcgagca
      601 tggcgtcgac aacgacgcga ctctggaaaa tttaggcaag caagccgtgg ttgcagctgc
      661 tgcaggcgca gacttcatcg ccccttccgc cgcgatggac ggccaggtac aggcgattcg
      721 tcaggcgctg gacgctgcag gctttaaaga tacggcgatt atgtcgtatt cgaccaagtt
      781 cgcctcttcc ttttatggcc cgttccgtga agctgccgga agcgcattaa aaggcgaccg
      841 caaaagctat cagatgaacc caatgaaccg tcgcgaggcg attcgtgagt cactgctgga
      901 tgaagcccag ggcgcagact gcctgatggt taaacctgct ggagcgtacc tcgacatcgt
      961 gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg tatcaggtga gcggtgagta
     1021 cgcgatgatt aagttcgccg cgctggcggg tgctatagat gaagagaaag tcgtgctcga
     1081 aagcttaggt tcaattaagc gtgcgggtgc ggatctgatt ttcagctact ttgcgctgga
     1141 tttggctgag aagaagattc tgcgttaatt tttttcctct cagaaagggg gagttaacct
     1201 gttgaaagta cggtgaacgc cccctcaccc taaccctctc cccaaggggg cgagggaacc
     1261 gtccacactc gtatcacccc gcccgataaa acggtttatc cccaaggatc atcgcccgat
     1321 gcataatccg ccgctgtggc aggtaatcgg cattggcata gtgctgagtc acgcggttat
     1381 cccaaatcgc aatatcattc ggttgccagc gccaacgcac ctgaaactcc ggtttagtga
     1441 tatgggcgaa taaaaagcct aacaaggctt cgctctcttt ctcgctgaca tcgacaattc
     1501 gcgtagtaaa gccttcattc acaaacagcg cctgtttacc gctcaccgga tgcgttcgca
     1561 ccaccggatg tagcaacgga ggatttttcg cgaccgcctc gcgccagcgt tgatgttcct
     1621 cctcggtttt gcggtatttg tattccggga atgatttacg gaaatcatgc tccgcacgca
     1681 gccagctcag cagctggcgg aagggaacag agagcgcctc ataggccgca ataccgctgg
     1741 ttcagagcat atcaccgccg gtcgaaggta actctttagc tgccagaatc gcccctgcgg
     1801 gtggcgtttc aataaatgtc acatcggtat gccagttgtc gttatccggc ggattatcgt
     1861 tatgggtatc cagcacgatg atctcgtcaa ccccttcggc atgagggtaa acagggtgaa
     1921 tatgcagttc gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta atagcttgat
     1981 cgcgcagaaa taccacctga tggcgcagca ccgcatggta aagctgttca aactgattat
     2041 cgcttaacgg gcgcgtcagg tcggcacccg aaatttgtgc gccgatatgc ggccccagcg
     2101 gggtaatgct cagacgttca cccattgtac ttctccatgc cagggcgtca gacggcgctg
     2161 taacgcgcgc agacccagtt ctaaaagaaa ggcgataatc gcaatcaccg cgatccccgc
     2221 cagtaccacg tcagtcgcga gaaattcacc cgctgactga accataaatc ctaaaccgcg
     2281 tgtcacggca atcagctccg ccgctaccag cgtagaccag cccacaccca gaccaatgcg
     2341 taatccggtg aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc
     2401 acggctggca cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc
     2461 tgccagcgcc gacatcgcca ccggcgcaaa aatcgctaaa tagatcagta agatcttcga
     2521 ggtttcacca ataccaaacc agatcaccat cagcggcaaa taagccagcg gtggcaccgg
     2581 acgataaagt tcgattaccg gatccagaat gccgcgcacc gtcgggctta gccccatggc
     2641 gatcccgacc ggaataccga tcaccaccgc cgcgagtagc gcctgcacaa tgcgcgtcag
     2701 gctggctgcc agatgctgcc acagcgtggc gtccataaat ccctgcggtc cggcaatggt
     2761 gagcagtttt gccagtactt gttgcggcgg cggcaaaaac agcgggctaa tcagttgcag
     2821 cgccgccacc gtccaccata cagtgagtaa aaccgctaac gtgccaatgc ttaaggtcac
     2881 ctgacgcgag agcggccagc gccatttcag ccgccgcgaa tgcagttttt cattaatgag
     2941 cacactcatg agaacgcctc ccgttgctca aatacgcggc ttaaaacata ttcgcgcatg
     3001 gcgatgaatt gtggatcgga cttgatgctg cggctcgact ctcccgcaac aaagcggcga
     3061 gcaaagttga gcggcagccg ctccagcaca cggccagggc cggatgaaag cagaaccagt
     3121 tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa tcaacagcac ctgcttgccc
     3181 gtctcctgcc agagtttcag cagcagagtt tgcatctggt cgcgggtgaa ggcgtccagc
     3241 gcaccaaacg gttcatcgag taataacagc tggggattcg ccgccagcgc gcgggcaatc
     3301 cccacccgct gacgttgacc atcggaaagc tgccagatat agcgtttttc tgcgccttcc
     3361 agccccactt ttttcagcat ctggtgcgcg atttccagtc gctgcatttt ctctatacct
     3421 gccaattgca ggccgaacgc cacgttgtcc tgtacattgc gccacggtag cagcccttca
     3481 ttctgaaaaa ctacgccacg ctctgctccc ggtccctcaa tacgcttacc cgccagttga
     3541 atgctgccat gctgataagg cacaaaaccg gcaatcagat tcagcagggt ggttttaccg
     3601 cagccggacg gccccagcac caccagtagc tcgccgcttt ccagcgtcag gttgatatct
     3661 tccagtgccg gtttgccgcc ataatcggca tagagatgag agatttgcag catctgcgcc
     3721 tccttttatt gcacgaagcg cgaggtaacg tactggctgt aatcattcgc tacagccggg
     3781 accttgccct gctctttcaa aaactgcgcg gtgtcgatga ttgctttgtt caccggtccg
     3841 gtcagttctg ccgtttgttg ctgcggcgtc agataggtat ttcccttcac cagccccgga
     3901 acgtcacctt caggcacgcc gcttaaacgc gccagtttgc tgatgttttc cggctgtttc
     3961 agccacgcgt cagggttagc aatgtacggt tgctgtgcat cgatggcgct tttggcgaac
     4021 gctttcacga cctcaggatg tttctcggca aaatctttgc gcaccaccca gacgtccagc
     4081 gttggcgcac cccactgccc gacctgttca gaatcggtca gcaccttgtc gtctttttcc
     4141 agggcgttaa ccgccggtgc ccagacataa gcaccatcaa tatcaccccg ctgccatgcg
     4201 gcgataatcg cgggcggctg caggttcaca atctccacta gccctggttt aatcccccag
     4261 tgtttcagcg ccgccagcag gctgtagtgg gtggtggaga taaacggtac ggcaatgcgt
     4321 ttaccaatca aatcttcggg tttgctgata gttttcttta ccaccagcgc ttcggagtta
     4381 cccagttttg acgccagcaa gaagacttca atcggcacct gttggctggc tgcaaccgct
     4441 aatgggctgg atccgaggtt gccgatttgc acgtcgccag aagccagcgc ccgcacgatg
     4501 ctggctccgc tgtcaaactt acgccagtcc acgctcgccc cgctctcttt agcaaaggtg
     4561 ttgtcggcct gagccacttt cgccggttcg gctgaggttt gatacgccac ggtgacgttc
     4621 accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt atatgactag
     4681 gtaatttatt caagataacc gggaatgggt aaaaaaagac gggatttgat gggattcaat
     4741 aagttatgcg ccgattgcat tcaagataaa acgagttaaa aatgctcaat gcttcgatat
     4801 cgataaaaca ttcaagatag gcgcgccgtg tattttaatt cttttaagac tcttcaaaaa
     4861 tgtcatcgta gcctttaaaa acgaatttta aaggcaccct aaaggcaggt caatgaagtt
     4921 aacccgcctt cgcgtttaca ctctctgaga gaatactagc ttcaatctct tccaaaatct
     4981 ctctgacttg ctcgtcgggc atatcaacga gcatcaacgc tgccaggcgt ttacggccct
     5041 ctagttgcag tagctcttga gtttccttat ctaacaagcg tagcacagtc tcaacacctt
     5101 tccttgcgag catgtttgta agggcttctc gttcctcact ggtaagaaac gacaacattt
     5161 ctgttagctg atccccttgg cttaaatcac tttgtgtttt ttgtgttttt ggtggattcc
     5221 cgttaccggt ttttagccat tcaagactaa caccttcact ttctgcaatg cgtaccaata
     5281 tgctttctcg aggttctggt gtatttccct ttttaaaata gctttgtaac gtattgatat
     5341 ttatgccaca ggctctggcg gcagcacttc ttgatggata ccgcctaata agataagcca
     5401 tgcgctccac gataggttct tttgcctgct ccataaaagt acctctaggt atttttctgt
     5461 ataaaggttc ttttccatct atctaattga atggaaaggt aattttaaaa atccaaataa
     5521 aatacaaaaa aataccttgt attggtgctt tttgtgatct gcgattttat ttactcggat
     5581 atccacgcga atatccgcgc gggtaatttt gtagggtaaa ggaaagatga gacaaagaga
     5641 agatagtggc cgcgattggc cttcgctgat gattcagcaa gcactcaaag ataatgggtg
     5701 gtcttccttt cgggagcttt cactccatgc cggttatgct caggattcac tgaaagcggt
     5761 agttcgtacg ccaaacaaaa aatacgaacg tgttatttct gacgctatag gtgtaccgcc
     5821 agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg cgtcgtgata
     5881 tgcagtcgta cgttaccgta aatgaccttc tcggtgttcc tggaatgcca gccaccgcga
     5941 aaggcatccg acaggcgtta cagcgttttt ctcgtgctct tggtgacgtt agtcgccgtc
     6001 gagcaggtac aaaagccatt gagtaccata tcgattgcct gcctgaggta acccaaaagg
     6061 cactgcgtga acgctatgtg gaacaactgg tggctacaga gaacaacgtc tccgaagtga
     6121 agaccgttac caggacggcg cgtaaccctg atgctgttca ggcagttgaa gcatatcggg
     6181 gttctccgca actaatggaa gaacgcctga atgccctgac tgaaaatcag cgccgggtgt
     6241 ctgaagcaag agcggcgctg gcagtggaag tgctgaagct ggaaagcgcc ggtaacctcg
     6301 ggcgactgaa agccattaac tttctggttg aaaaagcccg taaaggtgag ttgccggaac
     6361 gactgcaaca ggctgcgatt aacgctaatg caaaacgtgg cactaatcgc accatcagcc
     6421 gtgatccgct ttaccagtgg gtactgaaat acaaccggtc gcagaacgcc gcagaacgac
     6481 tgttgttact ggcaccagga aaacgcgacg aaataaaacc ggaagaaatt agctggctgc
     6541 ctgaatttct ggcgcagtat cgtcaggcta acggcagacc tatgtcggaa gcctacgagg
     6601 attttgtcac ggaatggcaa cgtcgctatg cggacgaacc gtacatgctg gaggtgatgc
     6661 cttcctatga tgttgtccgg tatgccatga aaaaattgcc ggaagtggtg aaacaaaaag
     6721 gacgcgttac cggcagtgaa taccgccagc ttgagggatt cacccgccgc gactggacag
     6781 ctatgccggt gaattatgtc tggattggtg acggccacgg tatgaagctg aaatgtgcgc
     6841 atccgatcca cgggcgtcca ttttcaccgg aagtcacgtt tgttatcgac ggtggaaccc
     6901 ggtttgttgt tggctggagt cttgatcttg cggagaacgt gtttgctgtt gccggggcga
     6961 tacagcacgg aattcgcaat cacggaaaac ccttcctgta ttactcggat aatggttccg
     7021 gtgaaacggc agatatgctg gataaagaga tcgtcgggat tctgccccgt ctgggtatta
     7081 accaccccac cggtattgcc ggaaatccgc agggacgcgg catcattgaa cgtctgaacc
     7141 ggacgctgcc gatgcgtata gccagacggt atcgcaccta ttttggcaag ggggcagacc
     7201 gggaaacact gcgtgtactc aatcgcgatc tgcgttcagc atttaatgcc ctgcaacagg
     7261 acaaaccgct gaatgcccgc cagaaatctg caatgcgcga gctgccgtcc tggtcagaac
     7321 tgattgaggc tattcgtgaa ggagtggaat ggtacaacaa ccgcccgcat tctgaactgc
     7381 cgatgaaacc aaacgggaag cattacagcc cggctgagtt cagaaaaaaa cgcctggcgg
     7441 aagaaaacac cgaaattgaa tggctgagtg atctcgaact gcgcgacatg ttctgcccga
     7501 tggtggagcg tccggtaaga cgctgtgaaa tcaaatggct tgggaatatt tattacgcgc
     7561 cggagctgcg tgatgaacat ggtcgcaaag tgcttatcag ctatgacatt catg
//
LOCUS       DAJTYI010000164         7570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYI010000164 DAJTYI010000000
VERSION     DAJTYI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(65..604)
                     /locus_tag="OO564_003574"
     CDS             complement(65..604)
                     /locus_tag="OO564_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6654536.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(669..1217)
                     /gene="fimA"
                     /locus_tag="OO564_003575"
     CDS             complement(669..1217)
                     /gene="fimA"
                     /locus_tag="OO564_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6654537.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1698..2294)
                     /gene="fimE"
                     /locus_tag="OO564_003576"
     CDS             complement(1698..2294)
                     /gene="fimE"
                     /locus_tag="OO564_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6654538.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2772..3373)
                     /gene="fimB"
                     /locus_tag="OO564_003577"
                     /pseudo
     CDS             complement(2772..3373)
                     /gene="fimB"
                     /locus_tag="OO564_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4827..5543
                     /gene="nanC"
                     /locus_tag="OO564_003578"
     CDS             4827..5543
                     /gene="nanC"
                     /locus_tag="OO564_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6654539.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5563..6669
                     /gene="nanM"
                     /locus_tag="OO564_003579"
     CDS             5563..6669
                     /gene="nanM"
                     /locus_tag="OO564_003579"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6654540.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6734..>7570
                     /locus_tag="OO564_003580"
     CDS             6734..>7570
                     /locus_tag="OO564_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6654541.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLS"
BASE COUNT         2260 a         1616 c         1557 g         2137 t
ORIGIN      
        1 tccttacatt gacgttttta ttactcactg tcctgttcct gttatcacat tatctactga
       61 acaattactg ataggttaaa gagaaccagg cctgggcatt ggcgatgccg ccagtaaccc
      121 gacgcccggt agcacgatat ttggcgatga aatgtagcga agtagagcct gagtaaagcc
      181 gtttccagtt tgctggagga cgattaatcg gtacgaggtt tccttcatca tcaaacaacg
      241 ctacaccaat attggaggct atccctggcc cctctcccac ggagagcaca tccggatttt
      301 taccatccgc gacaccgtga aacgccacac ccacacgttc actcaccacc gtgctacatt
      361 cccgtaaatg aataacaaaa ggcaccggtg cgctatcttc ccctgccgca tgaaaccggt
      421 tactgctgat ttgccccata ttgaccgtca tttgtttatc accggcttca atccggcaag
      481 tttccgcaat aatgacgccc tgaaattgca tatttccgcc gggcagcgtg gtattccatt
      541 tatttccggc cagagcaaac attggcagca acgacgctaa cagaaataat ctcttccttt
      601 tcatcgttat ttttatcgca caagggtggg catccctgcc cgtaatgacg tccttgaacc
      661 tgggtaggtt attgatactg aaccttgaag gttgcatccg cattagctgc acccggggtt
      721 gcctcgccga ttgcataata acgcgcctgg aacggaatgg tgttggtacc gttattcagg
      781 gttgtttgtg cactaaatgt cgcaccgtcc agcgtcagag cattgcctgt tctgtccagg
      841 atctgcacgc caacgtttgt tgcactacct gcagcagaac tctgcagagc tagtacatca
      901 gtacgcgtag catcaattgc cgtacctaag aaggcaacag cggcttttgt ggcaacagtg
      961 gtatcgcaat cattcagctg aatgttaaaa ccaacggcag agctggttgc tccagcctgc
     1021 ttcaggctag cggtacgaac ctgtcccaac tgaacggttt gatcaacaga gcctgcatca
     1081 actgcgcaag cggcgttaac aacttcccct ttaaagttaa tggtcccacc atttaccgtc
     1141 gtagtatcgg ccagagccgc tgcggaactg agggacagag ccgacagaac aacgattgcc
     1201 agagttttaa ttttcatgct gctttccttt aaaaaaacta tttctaaatc gacatgggca
     1261 gtcgttctgt acactttgtt ttgtcaacga gtttgactgc caacactgca cagttttccc
     1321 ccaaaagatg aaacatttgg ggccattttg actcatagag gaaagcatcg cgcacaaact
     1381 ttttcagttt atttgttggc ttaatattct attgttatct ttatttatag atttttatat
     1441 tgcatgaggt ggtttttgga gagaagaatg aggaagatgc gtcgagccac agaaacgtta
     1501 gctttacata tagcggaggt gatgtgaaat taatttacaa gagaaataat ttacatatca
     1561 aatagttaga tgctttttgt cgttttttaa tatttttatg cttgagaaaa aatacgtaac
     1621 ttatttatga tatggacagt ttggccccaa ttgtcttgtg tttatttgtt ttttaacttt
     1681 attatcaata agttaaatca aacctcttct ctttttaatt tttcgtttat gagattattt
     1741 ctttcccata atccggcaaa acgagcagca ttactggcgg tataacgcac agtatggcga
     1801 atatttcgat gcccgagata atcctgaatt aaacgggtat ctgcaccacg ctccgccagt
     1861 tcataaccgc aagcatgcct taacatatga ggatgagtct gcgttacggt tccagcttca
     1921 ataccggcat cgcgaataat gcgataggcc tgctggcgag aaagccgact cccgcggcga
     1981 gaaataaata tagcgtcggt ccggtcagcg cctttccagt tagcacgttc ctgggtccag
     2041 cgttccacgg cttcacgctc atcaaaacgt aacgggtgaa cggtagaaaa tccgttcttc
     2101 agtcggcgaa tatttattcg accttcatta aggtcaaggt cctgataatg cagatcaagc
     2161 agttcactaa tacgcatccc atgccgatat gccaacagaa taagacaata atctctggct
     2221 cccgttgccc cgtaacaaac cgcctgcatc atggcctgaa cttctttacc ggtaagataa
     2281 cgacgtttac tcacaatagt agtactccta actgagatat atttgaatga atacctatag
     2341 gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag ataaccacac acgaagttat
     2401 gtggctatca gtcaattaca ttaaacatca tacaaaaata aaatatcact gatgtgacaa
     2461 catctatttt cattgatttg attcaatata acaatagatt acagaggatt aacttccagt
     2521 tacttaaaaa ataaagaaaa aagtgtggaa acaggagaat ttaagtgatg tgtaaactta
     2581 tgaatgattt tgtttattta ttttgaagaa cataaaccat tagattttgt atgggtatgg
     2641 ttaacttaat gtttattatg ataattaaga atcacactta caataacatc aattaataaa
     2701 tggttaatca tttttgatat atcgtaagaa taatgtagtt tttaacacca tccctggtat
     2761 ctcaactatc tctataaaac agcatgacgc tgtcgtcctc tggctctatc ccagatgccg
     2821 taaaaacgcc ctgcattgct ggcggtatac cagacagtat gacgaatatt gcgatgccca
     2881 agataatcct agataagtcg cgtatctatt cctatattcg ccaaagcaaa accacacgaa
     2941 tagcgtaaca tgtgcggatg aatctccagt gacaacccgg cattaccacc agaagtcgag
     3001 ataatatggt aaaactgttg ccgagaaagc ggattcccct tacgtgataa aaatacccac
     3061 tcgctctcag catgcggata agaagtacgg atactcaacc agttttttaa agcctgaact
     3121 tctttgttca atagcgggtg cgttgttgaa aagccttttt ttaatcgatg gatatatata
     3181 cactttgctt taagatcaat atccgaaatc ctcaatcgac aaatttcact cgcccggaaa
     3241 ccatgaataa aacaaagcaa aatcagacaa taattacgtg ttgcatgagg ccggtatttg
     3301 ctgctttaag gagtgactcg atttcactat gggtaaggaa gttccttttt ttgttatcag
     3361 ccttattcct catcgttttc ccttataatt acagacgcgc actagctgtg ctgggttagc
     3421 aataatccaa catttttatc ctgattatgt ttataaaagc gaacgtttgc ttaataactt
     3481 acgtaagtgg accagttctt ctgagcgaac ttaaatggag tagcgatagt tacttataat
     3541 aactttgcca tacaaacgcc atgctcttgc atgctatgta cctttatata tttatcaatc
     3601 tgatacgaat ctaataataa ccctcatcat taatgcaact aatcttatct atatatcatg
     3661 tgatatgttc tgtaacaagt aaaatcaaca taaaaaattc agcaaaattt aatatttgtt
     3721 caactagccg tctcaaatat gacattatta gttaactgca tttgccacaa aatggctatt
     3781 tcatggaacg cagtaatttc ttagtggcta ttatcatgct aattcttaga tccctagcga
     3841 ttattcctgc ctggttcgtc agcttaggcc atgagattgt tacattcctt tttatcctgg
     3901 agatatgggg gaacgcaggc gacgcttttt catctacatc cgttggtaaa tatagctttg
     3961 gtcactacga cttaagcctt cctcaatcac agctattcgc cgggcgatac ggcaaacaaa
     4021 ctaagtgaaa catcatcctg aaaacaccaa catcaacaag cctctccaga tcgacatcag
     4081 gagtgaccag ttacaagcca caaaccaaga aacatatttc atgatgagaa ttatgctcag
     4141 cctgatggcg agtaaaccct tattttcacc actctcactg agttagcttg caaatctttc
     4201 tttgtttaga ttctaaataa attttttata aaacatatag ttaaaagaaa atctttagtt
     4261 tttgaagacc accgcaagtg ttcgtctggc ttcatatggc atcttcttct tagaaaaaga
     4321 tcgacatatt ttgtgacgcg aattgcaaat ctggttttgt tgtatggatt gcgtgatttt
     4381 tgatctggta taacaggtat aaagatgcac cacgatagtc aatgagactg ggcatctcgt
     4441 aatctatggc aaacatcact tcagttcttt cacatcgggt gatgaaaatg cacttcagtc
     4501 tgaaaggaat atgaaaatga gatcaacaga cactctattt tatgactctg ggtaaaatgg
     4561 gttgagtaag tgatataact tacgaatatt caaatcaatt aaaaatcaga agagatttta
     4621 tactcaggta tttaatctgg atctctgttt atttaaataa tgttaaaaga gaattttcac
     4681 aggagacctt atacaaaaaa tataaaatac agttacccgt tgccaaagac actataagcc
     4741 tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt gtattgcttt
     4801 ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct ggcgtattat
     4861 tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta cgtggtggat
     4921 atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga tggcaaaatg
     4981 gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat aaaaaggaaa
     5041 atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt gatgatcaat
     5101 ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc tacggaccct
     5161 acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc tatcgttacg
     5221 actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat aacgttcatc
     5281 gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca tggcaaacga
     5341 cgctatacag caaacagaat gattatcgct atgcaaacca caagaaatgg gcgacggaaa
     5401 atgcctttgt tctacaatac catatgacgc ccgatattac gccatacata gaatatgact
     5461 accttgaccg ccagggtgtt tacaacggca gagataattt atcggaaaac agttatcgca
     5521 ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa aactataatg
     5581 gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc ggaaactcct
     5641 gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat tggtttaggt
     5701 agcgcaggta cagcatggta caagctagat acacaggcca aagataaaaa atggactgcg
     5761 ttagctgcat tccctggtgg accgagagag caagcaacct ctgcatttat tgatggcaat
     5821 ctgtatgtgt ttggcggcat tggcaaaaac agcgagggat tgactcaggt atttaatgac
     5881 gtacacaaat acaaccccaa aaccaatcgc tgggttaaat tgatgtcgca cgcgccgatg
     5941 ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg cttatgttac tggcggtgtt
     6001 aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa agattcaacc
     6061 gctatagata aaatcaacgc ccactatttt gacaaaaaag aagaatatta tttcttcaat
     6121 aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg cgaatcgccc
     6181 tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg gcttattaat
     6241 ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt caccggtaat
     6301 aatttaaaat ggaataagct tgatcccgtc tcatcaccag atggcgtcgc tggcggtttt
     6361 gcggggataa gcaatgattc tcttatattt gccggagggg ccggattcaa aggttcacga
     6421 gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc atatagcact
     6481 gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc gcaaggtcgg
     6541 gcctacggag tatcattgcc ctggaataat agtttattaa ttattggcgg tgaaactgca
     6601 ggcggcaaag cggtgacgga ttcagtttta atttctgtga aggataataa agtcacagta
     6661 caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt catttgtatt
     6721 acgcgaaaat tatatgaacg caataatatc gcccgattat tactatgttc ttaccgttgc
     6781 tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca gggaagatgc
     6841 gcctcattcc agaattaaac aattagcgag atttgcgcat acgcatcccg gaggcccgcc
     6901 atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc aggatatgca
     6961 gggttatcac catcctctgg caacgaatca tcaaacacag tacggcaccg ttggccaggc
     7021 actgcatatt gcacggaaat tactgccctt cattcctgat aatgcaggga ttcttatcgt
     7081 tccgtgttgc cgtggcggat cggcttttac cgcgggcagc gaagggacat attcagaacg
     7141 gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctgt accaggattt
     7201 agtcagcaga acgcgagccg cactggcaaa aaatccgcag aacaaattcc tcggcgtatg
     7261 ctggatgcaa ggcgaatttg acttaatgac cagtgactac gcgtcacacc ctcaacactt
     7321 taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc agcttaataa
     7381 tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag aaaatttccc
     7441 gcattcgtat gaagctattt atggcaatta tcaaaataat gttttagcca atattatttt
     7501 cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg aagacccgga
     7561 cgatttaagc
//
LOCUS       DAJTYI010000165         7504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYI010000165 DAJTYI010000000
VERSION     DAJTYI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1101
                     /gene="gmd"
                     /locus_tag="OO564_003581"
     CDS             <1..1101
                     /gene="gmd"
                     /locus_tag="OO564_003581"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6654542.1"
                     /translation="TGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDP
                     HTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMG
                     TLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWI
                     TVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLR
                     DWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEE
                     KGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLRE
                     MVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1104..2072
                     /gene="fcl"
                     /locus_tag="OO564_003582"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1104..2072
                     /gene="fcl"
                     /locus_tag="OO564_003582"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6654543.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2072..2551
                     /gene="gmm"
                     /locus_tag="OO564_003583"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2072..2551
                     /gene="gmm"
                     /locus_tag="OO564_003583"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6654544.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            2548..3771
                     /gene="wcaI"
                     /locus_tag="OO564_003584"
     CDS             2548..3771
                     /gene="wcaI"
                     /locus_tag="OO564_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6654545.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            3774..5210
                     /gene="cpsB"
                     /locus_tag="OO564_003585"
     CDS             3774..5210
                     /gene="cpsB"
                     /locus_tag="OO564_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6654546.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            5315..6685
                     /gene="cpsG"
                     /locus_tag="OO564_003586"
                     /gene_synonym="manB"
     CDS             5315..6685
                     /gene="cpsG"
                     /locus_tag="OO564_003586"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6654547.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            6740..>7504
                     /locus_tag="OO564_003587"
     CDS             6740..>7504
                     /locus_tag="OO564_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="WcaJ; glucose-1-phosphate transferase responsible
                     for the addition of the first glucose-1-phosphate from
                     UDP-glucose to the lipid carrier undecaprenyl phosphate in
                     the biosynthesis of colanic acid; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6654548.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNG"
BASE COUNT         1757 a         1942 c         2175 g         1630 t
ORIGIN      
        1 accggtgtaa ccggacaaga cggttcttac ctggcagagt ttctgctgga aaaaggttac
       61 gaggtgcatg gtattaagcg tcgcgcatcg tcattcaaca ccgagcgcgt ggatcacatt
      121 tatcaggatc cgcacacctg caacccgaaa ttccatctgc attatggcga cctgagtgat
      181 acctctaacc tgacgcgcat tttgcgtgaa gtacagccgg atgaagtgta caacctgggc
      241 gcaatgagcc acgttgcggt ctcttttgag tcaccagaat ataccgctga cgtcgacgcg
      301 atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa gaaaactcgt
      361 ttctatcagg cttccacctc tgaactgtat ggtctggtgc aggaaattcc gcagaaagaa
      421 accacgccgt tctacccgcg atctccgtat gcggtcgcca aactgtacgc ctactggatc
      481 accgttaact accgcgaatc ctacggcatg tacgcttgta acggaattct cttcaaccat
      541 gaatccccgc gccgcggcga aaccttcgtt acccgcaaaa tcacccgcgc aatcgccaac
      601 atcgcccagg ggctggagtc gtgcctgtac ctcggcaata tggattccct gcgtgactgg
      661 ggccacgcca aagactacgt aaaaatgcag tggatgatgc tgcaacagga acagccggaa
      721 gatttcgtta tcgcgaccgg cgttcagtac tccgtacgtc agtttgtgga aatggcggca
      781 gcacagctgg gcatcaaact gcgctttgaa ggcacgggtg ttgaagagaa gggcattgtg
      841 gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg gtgatgtgat tatcgccgtt
      901 gatccgcgtt acttccgtcc ggcagaagtt gaaacgctgc tcggcgaccc gaccaaagcg
      961 cacgaaaaac tgggctggaa accggaaatc accctcagag agatggtgtc tgaaatggtg
     1021 gctaatgacc tcgaagcggc gaaaaaacac tctctgctga aatctcacgg ctacgacgtg
     1081 gcgatcgcgc tggagtcata agcataagta aacaacgcat ttttatcgct ggccattgtg
     1141 ggatggtcgg ttctgccatc aagcggcagc tcgaacagcg cggtgatgtg gagctggtat
     1201 tacgtacccg cgatgaactg aacctgctgg acagccgcgc cgtgcatgat ttctttgcca
     1261 gcgaacgcat tgaccaggtc tatctggcgg cggcgaaagt gggcggcatt gttgccaaca
     1321 acacctatcc ggcggatttc atctaccaga acatgatgat tgagagcaac atcattcacg
     1381 ccgcgcatca gaacgacgtg aacaaactga tgtttctcgg atcgtcctgc atctacccga
     1441 aactggcaaa acagccgatg gcagaaagcg agttattgca gggcacgctg gagccgacca
     1501 acgagcctta cgccattgcc aaaatcgccg ggatcaaact gtgcgaatct tacaaccgcc
     1561 agtatggacg cgattaccgc tctgtcatgc cgaccaacct gtacgggccg catgacaact
     1621 tccacccgag taactcccat gtgataccag cattgctgcg tcgcttccac gaggcgacgg
     1681 cacagaatgc gccggacgtg gtggtatggg gcagcggtac accgatgcgt gaattcctgc
     1741 acgtcgatga tatggcggcg gcgagcattc atgtcatgga gctggcgcat gaagtctggc
     1801 tggagaatac ccaaccgatg ctgtcgcaca ttaacgtcgg cacgggcgtt gactgcacta
     1861 tccgcgagct ggcgcaaacc atcgccaaag tggtgggtta caaaggtcgg gtggtttttg
     1921 atgccagcaa accggatggt acgccgcgca aactgctgga tgtgacgcgc ctgcatcagc
     1981 ttggctggta tcacgaaatc tcactggaag cggggcttgc cagcacttac cagtggttcc
     2041 ttgagaatca agaccgcttt cggggggaat gatgttttta cgtcaggaag actttgccac
     2101 tgtagtgcgc tccacaccgc ttgtctctct cgactttatt gtcgagaaca gtcgcggcga
     2161 gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg tgccgggagg
     2221 gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag cggctgacga tggcggaact
     2281 ggggctgcgt ctgccgataa cagcaggcca gttttacggt gtctggcagc atttttatga
     2341 cgataacttc tccggcacgg atttcaccac tcactatgtg gtgctcggtt ttcgcttcag
     2401 agtagcggaa gaagagctgt tactgccgga tgagcagcat gacgattacc gctggctgac
     2461 gccggacgcg ctgctcgcca gtaacgatgt tcacgctaat agccgcgcct attttctcgc
     2521 tgagaagcgt gccggagtac ccggattatg aaaatactgg tctacggcat taactactcg
     2581 ccggagttaa ccggcatcgg caaatacacc ggcgagatgg tggaatggtt ggcggcacaa
     2641 ggtcatgagg tgcgggtcat taccgcaccg ccttactacc cgcaatggca ggtgggtgag
     2701 aactattccg cctggcgcta caaacgagaa gaaggggccg ccacggtgtg gcgctgcccg
     2761 ctgtacgtgc caaaacagcc gagcaccctg aaacgcttgt tgcatctcgg cagttttgcc
     2821 gtcagcagtt tctttccact gatggcgcaa cgtcgctgga agccggatcg cattattggc
     2881 gtagtgccaa cgctgttttg cacgccggga atgcgcctgc tggcgaagct ctctggtgcg
     2941 cgtaccgtgc tgcatattca ggattacgaa gtggacgcca tgctggggct gggccttgcc
     3001 ggaaaaggca aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg tagcggactg
     3061 cataacgtcg ataacgtttc cacgatttcg cgttcgatga tgaataaagc catcgaaaaa
     3121 ggcgtggcgg cggataacgt catcttcttc cccaactggt cggaaattgc ccgttttcag
     3181 catgttgcag acgccgatgt tgatgccctt cgtaaccagc ttggcctgcc ggataacaaa
     3241 aaaatcattc tttactccgg caatattggt gaaaagcagg ggctggaaaa cgttattgaa
     3301 gctgccgatc tcctgcgcga tgaaccgctg ctttttgcca ttgtcgggca gggcggcggc
     3361 aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca atttttcccg
     3421 ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg gcgattgcca tctggtggtg
     3481 caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat tctggcggta
     3541 ggcggtaacg cggtgattac cgcagaggcc cacacagaac tggggcagct ttgcgaaacc
     3601 tttccgggta ttgcggtttg cgtagaaccg gaatcggtcg aggcgctggt ggcggggatt
     3661 cgtcaggcgc tcctgctgcc caaacataac acggtggcac gtgaatatgc cgaacgcacg
     3721 ctcgataaag agaacgtgtt acgtcaattt ataaatgata ttcggggata attatggcgc
     3781 agtcgaaact ctatccagtt gtgatggcag gtggctccgg tagccgttta tggccgcttt
     3841 cccgcgtact ttaccccaag cagtttttat gcctgaaagg cgatctcacc atgctgcaaa
     3901 ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt tgcaatgagc
     3961 agcaccgctt tattgtcgcg gaacagctgc gtcaactgaa caaactcacc gagaacatta
     4021 ttctcgaacc ggcagggcgt aacactgcac ctgccattgc actggcggcg ctggcggcaa
     4081 aacgtcatag cccggagagc gacccgttaa tgctggtatt ggcggcggat catgtgattg
     4141 ccgataaaga cgcgttccgt gccgccgtgc gtaatgccat gccttatgcc gaagcgggca
     4201 agctggtgac ctttggcatt gtgccggatc taccagaaac cggttatggc tatattcgtc
     4261 gcggtgaagt gtctgcgggt gagcaggata cggtggcctt tgaagtggcg cagtttgtcg
     4321 aaaaaccgaa tctggaaacc gcccaggcct atgtagcaag cagcgaatat tactggaaca
     4381 gcggcatgtt cctgttccgc gccggacgct atctcgaaga actgaaaaaa tatcgcccgg
     4441 atatcctcga tgcctgcgaa aaagcgatga gcgccgtcga tccggatctc gattttattc
     4501 gtgtggatga agaagcgttt ctcgcctgcc cggaagagtc ggtggattac gcggtcatgg
     4561 aacgtacggc agatgctgtt gtggtgccga tggatgcggg ctggagcgat gttggttcct
     4621 ggtcttcatt atgggagatc agcgcccaca ccgccgaggg caacgtttgc cacggcgatg
     4681 tgattaatca caaaactgaa aacagctatg tgtatgccga atcaggccta gtcaccaccg
     4741 tcggggtgaa agatctggta gtggtgcaga ccaaagatgc ggtgctgatt gccgaccgta
     4801 acgcggtaca ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt caccatgagc
     4861 atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc gacgcgggcg
     4921 accgctacca ggtgaaacgc atcaccgtga aaccgggcga gggtttgtcg gtacagatgc
     4981 accatcaccg cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc accattgatg
     5041 gtgatatcaa actgcttggt gaaaacgagt ccatttatat tccgctgggg gcgacgcact
     5101 gcctggaaaa cccggggaaa attccgctcg atttaattga agtgcgctcc ggctcttatc
     5161 tcgaagagga tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa acgtcgcatc
     5221 aggcaatgaa tgcgaaaccg cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt
     5281 cagggccaac tattgcctga aaaagggtaa cgacatgaaa aaattaacct gctttaaagc
     5341 ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatatcgcct ggcgcattgg
     5401 tcgcgcctat ggcgaatttc tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct
     5461 caccagcgaa accttaaaac tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt
     5521 gctggatatc ggtatgtccg gcaccgaaga gatctatttc gccacgttcc atctcggcgt
     5581 ggatggcggc attgaagtta ctgccagcca taatccgatg gattacaacg gcatgaagct
     5641 ggtgcgcgag ggggctcgcc cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct
     5701 ggctgaagcc aacgactttc ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat
     5761 caacctgcgt gacgcttacg ttgatcacct gttcggttat atcaatgtca aaaatctcac
     5821 gccgctcaag ctggtgatca actccgggaa tggcgcagcg ggtccggtgg tggacgccat
     5881 tgaagcccgc tttaaagccc tcggcgcgcc cgtggaatta atcaaagtac acaacacacc
     5941 ggacggcaat ttccccaacg gtattcctaa cccgctgctg ccggaatgtc gcgacgacac
     6001 ccgtaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgatg gcgattttga
     6061 ccgctgtttt ctgtttgacg aaaaagggca gtttatcgag ggctactaca ttgtcggcct
     6121 gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct
     6181 ctcctggaac actattgatg tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac
     6241 cggacacgcc tttattaaag aacggatgcg caaggaagac gccatctacg gtggcgaaat
     6301 gagcgcccac cattacttcc gtgatttcgc ttactgcgac agcggcatga tcccgtggct
     6361 gctggtcgcc gaactggtgt gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg
     6421 gatggcggcg tttccggcaa gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc
     6481 gattaaccgc gtggaacagc attttagccg tgaggcgctg gcggtggatc gcagcgatgg
     6541 catcagcatg acctttgccg actggcgctt taacctgcgc acctccaata ccgaaccggt
     6601 ggtgcgcctg aatgtggaat cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac
     6661 tctgctgacg ttgctgaacg agtaatgtcg gatcttccct taccccactg cgggtaaggg
     6721 gctaataaca ggaacaacga tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc
     6781 atcgttaatc tctatggtgc aacgcttttc agatatcacc atcatgtttg ccgggctatg
     6841 gctggtttgc gaagtcagcg gactgtcatt cctctacatg cacctgttgg tggcgctgat
     6901 tacgctggtg gtgttccaga tgctgggcgg catcaccgat ttttatcgct catggcgcgg
     6961 tgttcgggca gcgacagaat ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt
     7021 cagcgccgga ctggtggcgt tcaacaatga tttcgacacg caactgaaaa tctggctggc
     7081 gtggtatggg ctgaccagca tcggactggt ggtttgccgt tcatgtattc gcattggggc
     7141 gggctggctg cgtaatcatg gctacaacaa gcggatggtc gccgtagcgg gggatttagc
     7201 cgccggacaa atgctgatgg agagtttccg taatcagccg tggttagggt ttgaagtggt
     7261 gggcgtttac cacgacacga aaccgggcgg cgtttctaac gactgggcgg gtaacctgca
     7321 acagctggtc gaggatgcaa aagcgggcaa gattcataac gtctatatcg cgatgcaaat
     7381 gtgcgacggc gcacgagtga aaaaactggc ccatcaactg gcggatacca cctgttcggt
     7441 gctgctgatc cccgacgtct ttaccttcaa cattctccat tcacgtctcg aagagatgaa
     7501 cggc
//
LOCUS       DAJTYI010000166         7473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYI010000166 DAJTYI010000000
VERSION     DAJTYI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..2146
                     /gene="dinG"
                     /locus_tag="OO564_003588"
     CDS             <1..2146
                     /gene="dinG"
                     /locus_tag="OO564_003588"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6654549.1"
                     /translation="LTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAGEE
                     GRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKKII
                     PDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLKGD
                     LDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAEVV
                     VANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQLDL
                     FTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEHRF
                     AMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRALG
                     MFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWRSI
                     PHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFEPS
                     IDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGDQP
                     RYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVITE
                     GEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKRLL
                     DALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2174..3136
                     /gene="ybiB"
                     /locus_tag="OO564_003589"
     CDS             2174..3136
                     /gene="ybiB"
                     /locus_tag="OO564_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6654550.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3277..4362
                     /gene="hcxB"
                     /locus_tag="OO564_003590"
     CDS             3277..4362
                     /gene="hcxB"
                     /locus_tag="OO564_003590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6654551.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(4591..4851)
                     /locus_tag="OO564_003591"
     CDS             complement(4591..4851)
                     /locus_tag="OO564_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6654552.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5116..5382)
                     /gene="ybiI"
                     /locus_tag="OO564_003592"
     CDS             complement(5116..5382)
                     /gene="ybiI"
                     /locus_tag="OO564_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6654553.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(5456..6133)
                     /gene="ybiX"
                     /locus_tag="OO564_003593"
     CDS             complement(5456..6133)
                     /gene="ybiX"
                     /locus_tag="OO564_003593"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6654554.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6175..7473)
                     /locus_tag="OO564_003594"
                     /pseudo
     CDS             complement(6175..7473)
                     /locus_tag="OO564_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         1729 a         1876 c         2076 g         1792 t
ORIGIN      
        1 attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc aggaacagat
       61 ccccgacttt attccccgtg cgccgcagcg gcagatgatt gcggacgtcg ccaaaacgct
      121 ggccggagaa gaagggcggc atctggcgat tgaagccccc accggcgttg ggaaaacgct
      181 ctcctatttg atccccggca tcgccattgc ccgcgaagag caaaaaacgc tggtggtgag
      241 taccgccaac gtggcgttgc aggatcagat ttacagcaaa gatttaccgc tgctgaaaaa
      301 gatcattccc gatcttaaat tcactgccgc tttcgggcgt gggcgctacg tttgtccgcg
      361 taatctgacg gcgctcgcca gcactgaacc cacgcaacag gatctgctgg cgtttcttga
      421 cgacgaactg acgccaaata atcaggaaga gcaaaaacgt tgtgcgaagc tgaagggcga
      481 tctcgacact tataaatggg atggtctgcg cgatcatacg gatatcgcta tagatgatga
      541 tctctggcgt cgtttgagta ccgacaaagc cagctgcctc aaccgtaact gttactacta
      601 tcgtgaatgc ccgttttttg ttactcgtcg ggagattcag gaagcggaag tggtggtggc
      661 aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc gtactgcctg acccgaaaaa
      721 tctgctgctg gtgctggacg aaggtcatca cctgccagat gtggcccggg atgcgctgga
      781 gatgagcgct gaaatcaccg cgccctggta tcgactacag ctggacttgt tcacgaaact
      841 ggtcgctacc tgcatggagc agtttcgccc gaagaccatc ccaccgctgg cgatccctga
      901 acgtttgaat gcgcattgtg aagagttgta tgagcttatc gcctcgttaa acaacattct
      961 caatctctac atgcctgccg ggcaggaggc agagcaccgt tttgcgatgg gcgaactgcc
     1021 tgatgaattg ctggagatct gccagcggct ggcaaaactc accgagatgc tgcgtggtct
     1081 ggcggagtta tttcttaacg atttaagtga gaaaaccggc agccacgata ttgtgcgtct
     1141 gcatcggttg attttacaga tgaaccgcgc gctggggatg tttgaggtgc aaagcaaact
     1201 ctggcggctg gcgtctctgg cgcaatcttc cggtgcgccg gtgaccaaat gggcgacgcg
     1261 ggaagagcgc gaagggcagc tacacctctg gtttcactgc gtgggaatac gtgtcagcga
     1321 tcagctggaa aggctgctgt ggcgcagtat tccgcacatt attgtcacct ccgcaacctt
     1381 gcgttcgctg aatagttttt cgcgtttgca ggagatgagt ggtctgaaag agaaagcggg
     1441 cgaccgtttt gtggcgctgg attccccctt taaccactgc gaacagggca aaattgttat
     1501 tccccggatg cgctttgagc cttccatcga caacgaagag caacacattg ccgaaatggc
     1561 ggcctttttc cgtgaacagg tggagagcaa aaaacatctc ggtatgttgg tactgtttgc
     1621 cagcggacgg gcgatgcagc gctttctcga ctatgtgacg gatttacgcc tgatgttgct
     1681 ggtgcagggc gatcagccgc gttatcgctt agttgaactg caccgcaaac gcgttgccaa
     1741 cggtgagcgt agcgtgctgg tgggcttaca gtcatttgcc gaagggcttg atttgaaagg
     1801 tgatctgctc agccaggtgc atatccacaa aatcgctttt ccgcccatcg acagcccggt
     1861 ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc tatccgtttg aggtgcaaag
     1921 cctgccgagc gcctcgttta acctgattca gcaggttggg cgactgattc gaagccacgg
     1981 ttgctggggc gaagtggtta tctacgacaa acgcttgctg acaaaaaatt atggcaagcg
     2041 actactggat gcattaccgg tatttccgat agagcaaccg gaagtccctg aaggtatagt
     2101 taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat gtgagtcagg
     2161 taaggagtcg taaatggact atcgcaaaat aattaaagag atcgggcgcg ggaaaaacca
     2221 cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat gctcatatgc tcaatggtga
     2281 agtccctgac cttgagttgg gcggcgtatt gattgcgctg cgtatcaaag gggaagggga
     2341 agcagagatg ctcggctttt acgaagccat gcaaaatcac accatcaagc tgacgccgcc
     2401 tgcaggcaag ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta aacaggctaa
     2461 cctgacgcca ttattggcga ttctgctgca taaactcggt tttcctgtgg tggttcacgg
     2521 agttagcgaa gatccaactc gcgtgctgac agaaaccatt ttcgaactga tgggtattac
     2581 accaacgttg cacggcgggc aggcgcaggc gaagctcgac gaacatcaac cggtgtttat
     2641 gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct ggcggatggg
     2701 cgtgcgtaac agtgcacata ccctggcgaa actggcgacg ccatttgccg aaggtgaggc
     2761 actgtgtctt tccagcgttt cgcatccgga atacattgga cgcgtcgcga agttctttag
     2821 cgatatcggc gggtgggcgt tactaatgca tggtactgaa ggcgaagtgt atgctaatcc
     2881 gcagcgctgc ccgcagatca atctcattga ccgtgaaggg atgcgggtgc tgtatgaaaa
     2941 acaggaccct gctggtagcg agttactgcc acaagcaaaa gatccggaaa ccacggcgca
     3001 gtggactgag cgttgcctgg ctggcagcga agcgattccc gaatcgctga aaatccagat
     3061 ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc agcgatggcc tggcgcgcgt
     3121 taatcaggca ttttaatttt tatctcagcc ggatgagcca cgtcatccgg cttttacccc
     3181 accgcaatat gaaattcctg catctttatt gaccttcccg cgcccggcgt gcagcataaa
     3241 aaaacaacaa acacataaca taaacaggag ttaaccatgg aaagtggtca tcgctttgat
     3301 gctcagacgc tgcacagttt tattcaggct gtatttcgtc agatgggtag cgaggaacaa
     3361 gaagcgaaat tagttgccga tcatttaatc gcggcaaacc tggcagggca tgattctcat
     3421 ggtattggca tgatcccaag ctatgtgcgt tcctggagtc aggggcatct gcaaattaac
     3481 catcatgcca aaatcgttaa agaggcgggg gcggcagtca cgctcgatgg cgatcgcgca
     3541 ttcggtcagg tcgcggcaca cgaagcgatg gcgctgggga ttgagaaagc gcatcagcac
     3601 ggtattgctg ccgtggcgct acataactcg catcatatcg gccgtatcgg ttactgggcg
     3661 gagcagtgtt cagcggcggg gtttgtctct atccactttg ttagcgtggt cggtattcca
     3721 atggtcgcgc cgttccacgg tcgcgacagc cgctttggca ccaatccctt ctgtgtggtt
     3781 ttccctcgta aagataattt cccgctgttg cttgattacg ccaccagcgc cattgcattt
     3841 ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg cctgattgac
     3901 gttaacggcg tgccgacgac caatccggcg gtaatgcagg agtcgccgtt ggggtcgctg
     3961 ttgacctttg ccgaacataa aggctacgcc cttgcagcga tgtgtgaaat tcttggcggg
     4021 gcgctttctg gcggtaaaac gacgcataag gaaacgttgc aaaccagccc cgatgccatt
     4081 cttaactgca tgaccactat catcatcaac ccggaactgt tcggcgcgcc ggattgtaac
     4141 gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga cgataagccg
     4201 attttgctac cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagga gcaggggatt
     4261 ccattggatg cgggaagctg gcaggccatt tgtgatgcgg cgcggcagat tggtatgtcg
     4321 gaagagacgt tgcaggcttt ctgtcagcag ttagccagct aaaaaaaagc ccgtccagtg
     4381 gcggacgggc aaacaagggt aacataggat caatgagggt tagagcatat gcgtctgtcg
     4441 gcaaacagac agggaaatac ttgtgctgga cgtagcgtaa acgcctgatc cgtccaacgg
     4501 ttcggatttt gtaggcctga taagacgcgg tagcgtcgca tcaggcatct gttgccggat
     4561 gcggcataaa cgccttatcc gtcctacgaa ttacttataa attaccgcag taccgcttaa
     4621 tttattgttg ttggtggcgg aagtgatgct gtatccactg gcaccggctg ccgccgcttt
     4681 ctcagccagt ttcgcttcca gggcatcgag ggtggatgcg ccatcggcag aaaccacgcc
     4741 gattttattc atgttctgtg cctgggatgc cgttaccggt tccgccgcga acacgccaaa
     4801 tgacagagtt gaaagagcca tagcagcaac aacagtattg atagttttca taattaatct
     4861 ctcgcaggtg atttttgtat aagtgacgtt gtttcgtcga tgtgatgagt atcacgtttt
     4921 tttacgagag ataaaatcga agagaattga ccgccttgtt caaataaatt gattgatatc
     4981 taacatactg ataaaccatt tatttgttgt acttatggac ttgttttatg ccgcgtggca
     5041 gtcacagcag cgttaaaggc acggaatgcc acgcaaaata caaattatac tcgctatgca
     5101 tctcgccagt tgtagttaac gtaactggct gtctttcgaa cctctgcgat tatatcctgt
     5161 atacgctggt ttttgtaaat ctttctcctg ctgacaatga atacataagc gcacgccagg
     5221 aatggcttcc cgacgggctt gcgggatggg ggcaccgcac tcttcacatt catacaggct
     5281 ttcgccacgc ggaatttcac cccgagcgcg ggcaatcgca tcttcaattg tactgttgat
     5341 ctgttcgttg acggcgtcat cgttagccca agcggatgcc atatcgtcct ccccatatca
     5401 atgcttgtac agttaagtgt agctaatata gggacgaacc tgggcagttc aagcgtcaga
     5461 tctccgacca ttcccgcagc agattatgat aaagattgag cagcgacagg atctcttcat
     5521 tttcaccata gcggcttttc agcgactgaa tatttttgtc cagttcaaac agcatggcgc
     5581 gctttttatc atcgcggatc atcgactgga tccacataaa tgatgccact cgtacgccgc
     5641 gggttacggg tgtcacgcaa tgcaggctgc tggagggata caacaccaga tcacctgccg
     5701 ggagttttac ccgatgttgt ccaaaggtgt cattaacgac cagttcgccg ccgtcgtagc
     5761 tttgtggatc gcttaaaaac agcgtggcag aaaggtcagt gcgcatccag ccgttttgtg
     5821 gatggctgcg tactgcgcca tccacatgga aaccataggt ttcattgttc tgatagcgat
     5881 taaacagcgg cgtggaaagg gtacgcggca aggcagcggc aaagaacaat gcatgttggt
     5941 taaccgcgtt cagcacctca ttttgcagtg cggcgtataa cgcgctgcgg gtgtctactt
     6001 gctgattgtt cttaacttgt gcaccttgtg ctccggtggt gacgcgtcca tccacccatt
     6061 cggcttgttc cagttgttcg cgaaaatggg cgacgtcctg tggcgataac acgccgggaa
     6121 tgtggtacat catacaagtt ctccaaaaag tggggcctgc gccccacatc tgaatcagaa
     6181 atgcatattg gctgtgagca agaaggttct tggctcgccc gggtgataac ggtagccgct
     6241 cttgttgatt gaggcgacgt aatcggtatc aaacaggttg taaacgttta gctggaagtc
     6301 gagattgcga ttaacccgat accccagttt ggcatctgcg acccagtaac cttctgtaaa
     6361 tgctggcgtt cccaccgcgc cgtctgaacc tttatgtata ctgccgatat agcgtgcgcc
     6421 cgcgccaaca gagatatcat cggttgcctg atattggctc cataaggtga aggcgtgctc
     6481 cggggtatac ggcagcgatg aggaaccatc ctgggcaaca tctttgccgt ttttgatggt
     6541 tgcttttttc tgggtatagc cgccaatcac ctgccacgcg ggagtgatat tccctgccac
     6601 ggatatctca tagccttcga cgcgtttctt accgtattgc gagtaagtcc cgtcatcatt
     6661 ttgctcaact tcattttcga tatcagtacg gaacagcgcg gcggtgagca acaggcgttt
     6721 atccagaacc tgccatttgg tgccaatctc gctggtgttg gctttttgcg gtttaaaatc
     6781 ggtgcggttg gcactgttac cactgccaga ctgcgcaagg gcgaagttgt tgccgcccgg
     6841 aggctgctgg gaaacggcat agttaatata gacattgccg ttttccgtca ggtgatacag
     6901 cgccccggct ttccagttca ccagattgcc cgacttggcg gtgtcgacgg tggtgactgg
     6961 agaacctttt gccacaccag ctgggcaggt gatggcaccg cgtccgctgc cgccgcaggc
     7021 ggtggcactg tcatattcag tatgataatt atccagacgg atcccgccgt tcagctcaaa
     7081 atcacgggtg atttgcagcg tatcgaaggc gtaaattgcg aaggtatccg tctgaccatt
     7141 ggcgtttgcg ccgttgcgcg tcaggccgcc gggatgaatt ctgctgtcag gatgataaat
     7201 atttaccgcc ggtaacgtca ccggattaac gccatagtta gtctgcgtct cacgggtaaa
     7261 ttccacgccg gtactgacat catgaccgat agaaccggta tagaacgtcg aggtcaggtt
     7321 ggtctggttg gtgagaattt tattactcac atctttggta ttcgccgtgc gtgactaggt
     7381 ccagctattc acatcgctgg tgggctgagt aatattcgac gccccgccca taatcgccgt
     7441 catcaggtaa tcctgcttta cgcgcgacca acg
//
LOCUS       DAJTYI010000167         7394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYI010000167 DAJTYI010000000
VERSION     DAJTYI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            83..2263
                     /locus_tag="OO564_003595"
     CDS             83..2263
                     /locus_tag="OO564_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6654555.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2404..3687)
                     /locus_tag="OO564_003596"
     CDS             complement(2404..3687)
                     /locus_tag="OO564_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6654556.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3822..4892)
                     /locus_tag="OO564_003597"
     CDS             complement(3822..4892)
                     /locus_tag="OO564_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6654557.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4870..5088)
                     /locus_tag="OO564_003598"
     CDS             complement(4870..5088)
                     /locus_tag="OO564_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6654558.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5173..5796)
                     /locus_tag="OO564_003599"
     CDS             complement(5173..5796)
                     /locus_tag="OO564_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6654559.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6118..6825)
                     /locus_tag="OO564_003600"
     CDS             complement(6118..6825)
                     /locus_tag="OO564_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6654560.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(6773..7207)
                     /locus_tag="OO564_003601"
     CDS             complement(6773..7207)
                     /locus_tag="OO564_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6654561.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7280..>7394)
                     /locus_tag="OO564_003602"
     CDS             complement(7280..>7394)
                     /locus_tag="OO564_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6654562.1"
                     /translation="AELEEALRDKQALLEASEKRIAELEAQTVTVKEVGDA"
BASE COUNT         1715 a         1838 c         1821 g         2020 t
ORIGIN      
        1 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
       61 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
      121 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
      181 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
      241 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
      301 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
      361 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
      421 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
      481 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
      541 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
      601 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
      661 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
      721 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
      781 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
      841 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta
      901 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
      961 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     1021 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     1081 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     1141 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     1201 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     1261 acagccggtg tttatggatc tagccaaaaa aggtgtggta gcagatttga ttggcgcagg
     1321 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     1381 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     1441 agccaatggg cagatgtcag cggtggcgct gatggacgct cgttctatcg ctgcgactgc
     1501 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     1561 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     1621 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     1681 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     1741 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     1801 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     1861 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     1921 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     1981 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     2041 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     2101 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     2161 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     2221 ggcgggtagt ttgattaact tcaataaaaa ccatcagatg taaaaagcgc catgtgaatg
     2281 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     2341 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     2401 ctcttacttc ttcgcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     2461 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     2521 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     2581 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     2641 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     2701 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     2761 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     2821 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     2881 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     2941 accgagaatg ttaacgttgt taatctgtac ctggtcaccg tcagtacgca gtgctaccgc
     3001 cggatggtta cctgcatcta cactatcgcc cagcgtgttt tcgatggtca gattttgcag
     3061 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     3121 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     3181 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     3241 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     3301 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     3361 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     3421 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     3481 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     3541 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     3601 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     3661 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     3721 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     3781 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     3841 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     3901 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     3961 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     4021 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     4081 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     4141 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     4201 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     4261 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     4321 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
     4381 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
     4441 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
     4501 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
     4561 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
     4621 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
     4681 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
     4741 gcctgtatag cttcagtgat tgcgattcgc ctgtctcggc ctaatccaaa ctctttaccc
     4801 gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaaggtt agggggtaaa
     4861 tcccggcgct catgacttcg ccttcttccc atttctgatc ctcttcaaaa ggctacctgt
     4921 tactggtcga tttaagtcaa cctttaccgc tgattcgtgg aacaaatact ctcttccatc
     4981 cttaaccgga ggagggaata tcctgcactc gcgtacccat cgacgaactg tttcaaggct
     5041 ccttgggcgt cgctggcgtg cgttccactc ctgaagtgtc aagtacatcg caaagtctcc
     5101 gcaattactc gcaagggagc gagtattggt ttattttctc caacaaaaaa ggagccgaag
     5161 ctcctttgat gattaaaatt cgaattgtct cgcccgaagg cttttcagca ttggttttgc
     5221 ccgttcgaaa agggcgcttg cttggtcaag tcgtgtcgcc tcccttagca gtacatctct
     5281 gtttttcgtc accgtgtaaa aagtttcaaa cgcgatgtca tacagcttgc tcgtgtatga
     5341 tgagttcagt tccttcaata tcgggtacag acgtttgcag atgtcctgcg cattctccat
     5401 ctgtacctgc atatagcaaa ggaggatgat ttcttcgtct gtgaattgct gcgctggttg
     5461 catgctgcga agtttctttt cgcactcgat gaagtatcgg cgtatctggc ggcctttttc
     5521 gttacgctcg accatcgccg tttctttggc tgtatcgagg gtgaggtgat agtctttgct
     5581 acggcgatcg cctccacgtc ctttgatccc cctgttttgg tgaaccaaaa tgtaatcgac
     5641 atcaacggca aagccgtatt ctgttatgcg atttttaatc catgtagaga agtcacgacg
     5701 tacttccaaa aattcgtgta aatcacgggc attaacgaga agagttgttt cgttggatat
     5761 agtgccgttg aatacgggga tgagttgact ggtcattttt atgtcctttg catttgttca
     5821 gatagccctg tgttcagcag ggcggtcagg tacttgaaca ccgtgcaaag tcggcccgta
     5881 tccttaacct ttcggctgtt tttcggtata cgcgctaccc gaccatatct gaaaaatgga
     5941 cataaaaaat ccgcatgact gacgggtgcg gtttccgctt tgcaaggtgt gttcagcacc
     6001 atgaagcgga atatagcccc gttaatgcgg tgttgtcaaa tcatgtaggc cttatcttgc
     6061 tgtaagccgc gccattcggg cttttcccca catttgggga aaactatctt gtcaaaatca
     6121 tgcagccttt cgctctcctt cgatgagttc ctctattttg tgcactccag cctcgttgta
     6181 ccgaaatgtt tcaacctgct tgtcattgtg cgccgatttg tcgatgaacc acttcccgta
     6241 cttttctgtt ttgagcatgt aggtgttggc tatgcgtcct accttgttgg ctgaaatacc
     6301 gtagcgctgg ccgacttccg tagcagagta gtagtgctct gagatagctg gcagaggtac
     6361 aacatcgcga ccaacaatcg ggttgaacac tccagctact gctgcctgct ttgattctga
     6421 ggacagattc gggaacatat tcaccagtcg atccgccacg ttcaaagcta tttccatggc
     6481 tcgcgcacgg tctaggctac tttctggttg cggtactggt tgaggatggt tggcaacctt
     6541 ttcgcgctga ttgaagtagc cgtcctccag tttttcgaac acgtcccatg cctgatccgt
     6601 ttcgagcatc ttcgcgtgac gggcggctcc gcgctctgtc cagaggatga ggtggcgtgc
     6661 atttggggca cttttcacaa agtagtttaa agctactttg tgttttagcg cgcgcagctc
     6721 gtcaccaacg accttgtaga agtgtacccc ctcaacaaag cgcgacttgt tttcatgatg
     6781 gttttgccgg atgcgcacaa catccgttcc gtacaactgc gccaatgttt ggtgggtagt
     6841 tgtttttaaa gtcagctgct aacacatagc attgtcgctc tcccatttct ggcattcgct
     6901 cactacagct tatccaggca tccggagtta ccggcactgg ctgggcggta taaagcggtg
     6961 ttatatctgc ccgaaaatta catgctttat gcagccgcac ccaccgttcg acttctgctt
     7021 tgtcagaata cataccagtg aacgtgttat attcacggtc aatttgcgtg aaggttacct
     7081 tccacgccac cggctctgct gtcagcgatg ccagagcaat ttcataagca cggcgttcaa
     7141 cattgtctcg cacatccaaa ctgctgatac gctctttgat ttctttaatc agttctttgt
     7201 cgttcactta tgcctccgat tcccggatgt cacccaaatc aacgctacaa tgccaaccag
     7261 tgcaacgagc gcaaaactat caagcatctc caacctcttt aacggtaact gtctgtgcct
     7321 ccagttctgc tatgcgcttt tctgaggctt caagtaacgc ctgcttatcg cgtagcgctt
     7381 cttccagttc agca
//
LOCUS       DAJTYI010000168         7372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYI010000168 DAJTYI010000000
VERSION     DAJTYI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            172..1283
                     /gene="yneK"
                     /locus_tag="OO564_003603"
                     /pseudo
     CDS             172..1283
                     /gene="yneK"
                     /locus_tag="OO564_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1433..2623
                     /gene="ydeA"
                     /locus_tag="OO564_003604"
     CDS             1433..2623
                     /gene="ydeA"
                     /locus_tag="OO564_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6654563.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2648..3313)
                     /gene="marC"
                     /locus_tag="OO564_003605"
     CDS             complement(2648..3313)
                     /gene="marC"
                     /locus_tag="OO564_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6654564.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3525..3959
                     /gene="marR"
                     /locus_tag="OO564_003606"
     CDS             3525..3959
                     /gene="marR"
                     /locus_tag="OO564_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6654565.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3979..4384
                     /gene="marA"
                     /locus_tag="OO564_003607"
                     /pseudo
     CDS             3979..4384
                     /gene="marA"
                     /locus_tag="OO564_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4416..4634
                     /gene="marB"
                     /locus_tag="OO564_003608"
     CDS             4416..4634
                     /gene="marB"
                     /locus_tag="OO564_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6654566.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4665..5564)
                     /gene="eamA"
                     /locus_tag="OO564_003609"
     CDS             complement(4665..5564)
                     /gene="eamA"
                     /locus_tag="OO564_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6654567.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGIHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5828..6946
                     /gene="ydeE"
                     /locus_tag="OO564_003610"
     CDS             5828..6946
                     /gene="ydeE"
                     /locus_tag="OO564_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6654568.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            7073..7168
                     /gene="mgtS"
                     /locus_tag="OO564_003611"
     CDS             7073..7168
                     /gene="mgtS"
                     /locus_tag="OO564_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6654569.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1922 a         1794 c         1671 g         1985 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcaaac agcgccacgc atcacctgcc tttgatagtt cttgcagctc
      121 aaatgcccta tatagtgatg ttccgatgac ttatgactat atggggcaaa tatggttacg
      181 ccagtaagca tcagcaatta catatctctt cctgatgatt ttcccgctcg taatattgca
      241 cctcaagtaa aagaagtttt aaaggatttt attgatgcac tcagtaccat aatatgtgat
      301 gaagagtggc gcacgtcttt aaacaccaac tcagccacaa aaaagatatt taacaacctt
      361 gataacttat catatattca gaggacttct tttcggggta acgacacact atacaatgaa
      421 aaggttcagt ttaaactcac ttatcccgtc aagaatggaa gacacaaaga aaatattgaa
      481 tttcaggtag taataaattt aagccctatt tagataattt tcgccatgat ggagaaatta
      541 atattttttg cgctcccaac ccaaagcctg tcactatggg gcgcgtattt cagaccggcg
      601 tcgagcgtgt gctctttctg tttatgaatg attttattga acaatttcca atgatcaacc
      661 ttggcgcccc catcaaaaga gcgcatacac cacatattga acccctgcct ccggatcacc
      721 ataccgctgc agattactta cgccagtttg atttgcttgt cctgaatttt atctctcgcg
      781 gtaattttgt catactcccc cgattatgga ataactctga ggttcacaga tggttcgtca
      841 ataaagatcc taacttgatc accgccattc tcgacataac ggacagcgaa ttaaaagagg
      901 atttattgca aagcctgatg gattcattag gttctaacaa acatgtacaa cccgaagtct
      961 gcatctgctt cttatccctt ttagcagaac aagaatctcc tcattttcag gacttgtttt
     1021 tatttttcgc caatatgtta ttgcactatc accaatttat gaatcccaat gaaagtgatc
     1081 tgaatgacgt gttgatgcca gcatcattaa gtgatgataa aattatcaaa catatggcgc
     1141 gcaggaccct caaactattt gtaaaaaatg aaacgccgcc aaaagttact cacgaagacc
     1201 tggtgaaaaa caggcctcgc tcccctgtca gaccacctat acccgcaacc gccaaaacgc
     1261 cagacctccc tgaacgtcat taaaccgtga tgttaccgac tctctgacgc ggaacagaat
     1321 cagcgtcaga gaaacggaaa acgcgatcca gatcacaaat gcattgtatt cacatcatta
     1381 accgttttaa gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac
     1441 aaacactgtt tcccgcaaag tggcgtggct acgggtcgtt acgctggcag tcgccgcctt
     1501 catcttcaac accaccgaat ttgtccctgt tggcctgctc tctgacattg cgcaaagttt
     1561 tcacatgcaa accgctcagg tcggcatcat gttgaccatt tacgcatggg tagtagcgct
     1621 aatgtcattg ccttttatgt taatgaccag tcaggttgaa cggcgcaaat tactgatctg
     1681 cctgtttgtg gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gctttaccgt
     1741 tctggtgatc agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc
     1801 gtctctggcg atccgtatgg ctccggccgg gaagcgggca caggccttga gtttaattgc
     1861 caccggtaca gcactggcga tggtcttagg tttaccgctc gggcgcattg tggggcagta
     1921 tttcggctgg cgaatgacct tcttcgcgat tggtattggg gcgcttgtta cccttttgtg
     1981 cctgattaag ttacttccct tactgcctag tgagcattcc ggttcattga aaagcctccc
     2041 gctattgttc cgccgcccgg cattgatgag catttatttg ttaactgtgg tggttgtcac
     2101 cgcccattac acggcataca gctatatcga gccttttgtg caaaacattg cgggattcag
     2161 cgccaacttt gccacggcat tactgttatt actcggtggt gcgggcatta ttggcagcgt
     2221 gattttcggt aaactgggta atcaatatgc gtctgcgttg gtaagtacgg cgattgcgct
     2281 gttgctggtg tgtctggcac tgctgctacc tgcggcgaac agtgaaatac acctcggggt
     2341 gctgagtatt ttctggggga tcgcgatgat gatcatcggg cttggtatgc aggttaaagt
     2401 gctggcgctg gcaccagatg ctaccgacgt cgcgatggcg ctattctccg gcatatttaa
     2461 tattggaatc ggggcgggtg cgttggtagg taatcaggtg agtttgcact ggtcaatgtc
     2521 gatgattggt tatgtgggcg cggtgcctgc ttttgccgcg ttaatatggt caatcattat
     2581 atttcgccgc tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccattcgg
     2641 gcctttttta atggtacgtt ttaatgattt ccaggatgcc gttaataata aactgcacac
     2701 ccatacatac cagcaggaac cccatcaggc gggagatcgc ttcaatgcca cccttgccca
     2761 ccagccgcat aattgcgccg gagctgcgta ggcttcccca caaaataacc gccaccagga
     2821 aaaagatcag cggcggcgca accatcagta cccaatcagc gaaggttgaa ctctgacgca
     2881 ctgtggacgc cgagctaata atcatcgcta tggttcccgg accggcggta cttggcattg
     2941 ccagtggtac aaaggcaata ttggcactgg gttcatcttc cagctcttcc gacttgcttt
     3001 tcgcctccgg tgaatcaatc gctttctgtt gcggaaaaag catccgaaaa ccgataaacg
     3061 cgacgattaa gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca
     3121 ccagttgccc agcgtaatac gccaccatca tgatggcaaa tacgtacacc gaggccatca
     3181 acgactgacg attacgttcg gcactgttca tgttgcctgc caggccaaga aatagcgcaa
     3241 cagttgttaa tgggttagct aacggcagca acaccaccag ccccaggcca attgctttaa
     3301 acaaatctaa cattggtggt tgttatcctg tgtatctggg ttatcagcga aaagtataag
     3361 gggtaaacaa ggataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt
     3421 gaaccgattt agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctag
     3481 gcaatattat cccctgcaac taattacttg ccagggcaac taatgtgaaa agtaccagcg
     3541 atctgttcaa tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag
     3601 atcgtctgct taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc
     3661 tctgctctat ccgctgcgcg gcgtgtatta ctccggttga actgaaaaaa gtgttgtcgg
     3721 tcgacctggg agcactgacc cgtatgctgg atcgcctggt ctgtaaaggc tgggttgaaa
     3781 ggttgccgaa cccgaatgat aagcgcggcg tactggtaaa acttaccacc agcggcgcgg
     3841 caatatgcga acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa
     3901 acctgacggc ggacgaagtt gcaacacttg agcatttgct taagaaagtc ctgccgtaaa
     3961 caaaaaagag gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt
     4021 ttggactgga tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt
     4081 tcgggttact ccaaatgagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa
     4141 aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc
     4201 gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga
     4261 gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata
     4321 ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag
     4381 ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag
     4441 cagctgcgct tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg
     4501 ttacttcttg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt
     4561 taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc
     4621 aacacgctat gtagtttgtt ctggccccga catctcgggg cttattaact tcccaccttt
     4681 accgctttac gccaccgcaa gccaaataca ttgatataca gcccggtcat aatgagcacc
     4741 gcacctaaaa attgcagacc cgttaagcgt tcatccaaca atagtgccgc acttgccagt
     4801 ccgactacgg gcaccagtaa cgataacggt gcaacccgcc aggtttcata gcgtcccagt
     4861 aacgtccccc agatcccata accaacaatt gtcgccacaa acgccagata catcagcgac
     4921 aagatggtgg tcatatcaat agtaaccaga ctgtgaatca tggatgcgga accatcgaga
     4981 atcagcgagg caacaaagaa gggaatgatt gggattaaag cgctccagat taccagcgac
     5041 atcaccgccg gacgcgttga gtgcgacatg atctttttat tgaagatgtt gccacacgcc
     5101 caactaaatg ctgcagccag ggtcaacata aagccgagca tcgccacatg ctgaccgttc
     5161 agactatctt cgattaacac cagtacgcca aaaatcgcta aggcgatccc cgccaattgt
     5221 ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt
     5281 gcctgtaaca ccagcgaagc cagtccagca ggcataccaa agttaatggc gcagaacaga
     5341 aaggcaaact gcgcaaaact gatggttaat ccatacccca agagcaaatt cagtggtact
     5401 ttcggtcgtg caacaaaaaa gatggccgga aaagcgacca gcataaagcg caaaccggcc
     5461 agcatcagcg gtggcatgtt atgaatcccc actttgatga ccacaaaatt tagcccccat
     5521 acgaccacta ccagtagcgc caacacccca tcttttcgcg acattctacc gcctctgaat
     5581 ttcatctttt gtaagcaatc aacttagctg aatttacttt tctttaacag ttgattcgtt
     5641 agtcgccggt tacgacggca ttaatgcgca aataagtcgc tatacttcgg atttttgcca
     5701 tgcgtccgat ggttaataac aacgacgagg caagaagggc gctggtagag cgtcgtaggg
     5761 ataagttcat ttgtctgtcc ggcagtgcgt ttcgttatgt tttattttag agatgtaaag
     5821 aaatagcatg gctacgctgc catttatgac catttacttg agtcgccagt acagcctgag
     5881 tgtcgatcta atcggttatg cgatgacaat tgcgctcact attggcgtcg tttttagcct
     5941 cggttttggt atcctggcgg ataagttcga caagaaacgc tatatgttac tggcaattac
     6001 cgccttcgcc agcggtttta ttgccattcc tttagtgaat aacgtgacgc tggttgtgct
     6061 cttttttgcc ctcattaact gcgcctattc tgtttttgct accgtgctga aagcctggtt
     6121 tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact acaccatgct
     6181 aaacattggc tggaccatcg gtccgccgct cggcacgctg ttggtgatgc agagcatcaa
     6241 tctgcccttt tggctggcag ctatctgttc cgcgtttccc atgcttttca ttcaaatatg
     6301 ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt
     6361 tttattacaa gataaagcac tgttgtggtt tacctgctct ggttttctgg cttcttttgt
     6421 aagcggcgca tttgcttcat gcatttcaca atatgtgatg gtgattgctg atggggattt
     6481 tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct gccatggtgg ttacgttgca
     6541 atattccgtg ggccgccgac ttaacccagc taacatccgc gcgctgatga cagcaggcac
     6601 cctctgtttc gtcatcggac tggtcggttt tattttttcc ggcaacaacc tgctattgtg
     6661 gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat
     6721 gttgattgac catattgcgc cgccaggaat gaaagccagc tatttttccg cccagtcttt
     6781 aggctggctt ggtgccgcga ttaacccatt agtgagtggc gtagtgctaa ccagcctgcc
     6841 gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt
     6901 aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt tgttgattta agtcgaacac
     6961 aataaaaatt taattcagcc ttcgtttagg ttatctctgc taatatcttt ctcattgaga
     7021 tgaaaattaa ggtaagcgag gaaacacacc acaccataaa cggaggcaaa taatgctggg
     7081 taatatgaat gtttttatgg ccgtactggg aataatttta ttttctggtt ttctggccgc
     7141 gtatttcagc cacaaatggg atgactaatg aacggagata atccctcacc taaccggccc
     7201 cttgttacag ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa accgccagta
     7261 aaccggcggt gaatgcttgc atggatagat ttgtgttttg cttttacgct aacaggcatt
     7321 ttcctgcact gataacggat cgttgacaca gtagcatcag ttttctcaat ga
//
LOCUS       DAJTYI010000169         7253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYI010000169 DAJTYI010000000
VERSION     DAJTYI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..695)
                     /locus_tag="OO564_003612"
     CDS             complement(<1..695)
                     /locus_tag="OO564_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6654570.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTME"
     gene            complement(692..1570)
                     /locus_tag="OO564_003613"
     CDS             complement(692..1570)
                     /locus_tag="OO564_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6654571.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(1581..2488)
                     /locus_tag="OO564_003614"
                     /pseudo
     CDS             complement(1581..2488)
                     /locus_tag="OO564_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(2475..2708)
                     /locus_tag="OO564_003615"
     CDS             complement(2475..2708)
                     /locus_tag="OO564_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654572.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(2705..3562)
                     /locus_tag="OO564_003616"
     CDS             complement(2705..3562)
                     /locus_tag="OO564_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6654573.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            3587..3916
                     /locus_tag="OO564_003617"
     CDS             3587..3916
                     /locus_tag="OO564_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6654574.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            3906..4214
                     /locus_tag="OO564_003618"
     CDS             3906..4214
                     /locus_tag="OO564_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6654575.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4256..4432)
                     /locus_tag="OO564_003619"
     CDS             complement(4256..4432)
                     /locus_tag="OO564_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6654576.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4429..4839)
                     /locus_tag="OO564_003620"
     CDS             complement(4429..4839)
                     /locus_tag="OO564_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6654577.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(4836..5114)
                     /locus_tag="OO564_003621"
     CDS             complement(4836..5114)
                     /locus_tag="OO564_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654578.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5189..>7253)
                     /locus_tag="OO564_003622"
     CDS             complement(5189..>7253)
                     /locus_tag="OO564_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HCR6654579.1"
                     /translation="CRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSARE
                     FYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKACPLNEPILLAD
                     GTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVT
                     SRGFTKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDG
                     SLSNGSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLM
                     GCTAKNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLV
                     NSLGGSCRTRVKTGVTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRF
                     EGCGVFVRNVEKIGNAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKT
                     CIASLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIE
                     IFDYARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRK
                     GDSEEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLAS
                     PASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQE
                     NVTEY"
BASE COUNT         1738 a         1948 c         1671 g         1896 t
ORIGIN      
        1 atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc gatgatgtcc
       61 agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag gtctccgggc
      121 tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt cctggatttc
      181 tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac atcatcaata
      241 tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt ctgcgcatcc
      301 ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg cagcgcctca
      361 ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc aacatagtgc
      421 tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg ggcaagttcc
      481 ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc ctgtctgcag
      541 atgcctgtat agatatcctg atacgctcgt acagaaaaaa cgtccgctgg cagtgtggcc
      601 agaatatcca tcacttcagg atctgctccg cgcagaaaga acgcgccgat gacagcgcct
      661 tcaaggtcat cgttacgcca tactggagtt gtcatgcagc cacacctctg atacgagaac
      721 ggtaactggg ccagttaaac gacaaccagt tgcgtccccc gtctgtgatc ctgtcggcaa
      781 ttcggggact gatgaacgcc cacaactctt ccggtgagag gttactgatc agaatggtgg
      841 gcaagatact ttcgtacctg gcgttgataa tttcctgcaa aatagccatt tcagccgcgc
      901 tgccaaactg aacgccaact tcgtcgatga tcagcaaatc cattgacgca taacgctcaa
      961 taacttcatc cgctgttttt tcgctgtcat tccgccagca atttttcaca gcacgggtaa
     1021 ggcgcatcac gtcggtgatc tccacactgg ccagatagtt acggatgatg tgttttgcca
     1081 tagccacagc cagatgattt tttccggtac cacaactgcc ggtcataaca agactggtac
     1141 cgttctccag catatctggc caattctccg catagcggcg acaggccgca agatttctgg
     1201 ctgcgccagg attaacctcc agataattat caaactcgca gtcccgaaaa cgcagggcaa
     1261 ttccggcgtt atcagtcagc tcttccgcct tgatggacga cagctccatg gtcaaatcgt
     1321 tggcctcagc gattaagcag tcagggcagc atgaaatttt ttctctgtcc tcgccattac
     1381 gatcgctcca caccagtata tgcgtgtgat attcgccatg tttttcgcaa tatccgcgac
     1441 cttcacgcat caggcaggaa cgataaggcc atggcttttc gcccttctga gcaaatgcaa
     1501 tctctgcccg taactcatcc attcgcgcct gtagtctttt ttgttgttca cgcaggttaa
     1561 acgtcatcat cgctgtcacc tcagaatgtc agtttgtcac tggatttacc gaatttgtca
     1621 gacatggctc ccaggccagc caggacatcg acctgtcgct gtcgcccacc tccgggagcg
     1681 gctggctgtt gccagaagtc ttcgaagtga cgatcgggtc caaagaacgt cgcagcctgc
     1741 ttcacgaact gtgtgccggt atttcctgtg gcacgtaccc aggcagcata gcgtttcacg
     1801 ccatcaagca tggcttcagg ttttattccc tccctgagcc gggctttcca ggctttgaag
     1861 gctgcggatt ttgagttacc accagcacgt tttggatatt cctgccaggc ctgttcaaat
     1921 tccggtgaat attcctgtcg ggaagaacgc gctggtgcag acgcgtcagc agatgcgcca
     1981 atagtgtttt tactctctgt agtattctct gaagtaatct ctgttgtatt ctctgtaaga
     2041 tcgaaattgg ttttcccttc tccgcggcga ggggtttccc gtgtccgcgg tgaaggcttt
     2101 ccctcctccg cgaaattggg ttttacagtt tcccgaaaac gggtttcccc atttcgggaa
     2161 actgattgtt ttcattgata atttcattaa ggcgctcaca atctatacgg tagaacattt
     2221 tgtgctcaag acgcttgttg gtttcaacca aaatgcctct ggacacaaga tgcttacgcg
     2281 ctacagcctg ttgttcaaat gtaagtccgg tttcgtgttg tatctcttca cgcgttttat
     2341 gtacgccttc cgctgcatgt gctttatcct accagtaaaa aatctgacca aagaaaataa
     2401 cagcgtgcgg acttcccatg tatttaacga gcccagggta ataagcaacc ggatgtccaa
     2461 aatcgagcag aagatcagac ggacgcatag ccacctccca ggcgtttaaa catttttccg
     2521 ggctgaaacg ccaccagcgg ataactcagg gtatgagtac gtccctgaac ctggcagaca
     2581 accttctggc tttcggtact gaccaggcaa acccgcagaa cgtagccgtt gctggtggtg
     2641 aaccactgcc ccacacgggg gcaatggttg tatcggtgat acaggaaatt aacgatgtgg
     2701 cggatcatgg gagcacctcc ttgtcagaac cattcagcct ggaatcaaca agtgcagcac
     2761 caaaaacagc atcacctaca cggtcgtaca gtttgccagc cagcggagat tcaacggcct
     2821 taagcattgg gtaaagctgg cttgtccaga tttgatggat ttcacgcaaa tgcaggtata
     2881 cgcctctggc gttttgtgcg acagatgaca tgtcagccgc accagctcct gataagctcc
     2941 tctccatctg gttaaaggca ttgatgtatg cctctttgaa ccgggcggca cgtttaccag
     3001 tgaagcccat agcaaggaag gcgaagccgt cgcgggtaat ttggtagcag ggaagtttgc
     3061 gagtaccgcc gttgggctgg cgtaccaaaa ttgatgtctc cgcaaaattg cgggcacaaa
     3121 actctggaga acaatccaaa atgcggatct ttttcagaac atcgtcatga cgtttagaga
     3181 agaagttggc aacagccagg gatgaagtaa cagcctgacc atcaacgatg gcaatttcag
     3241 gttgagagag ggttgggaga gtagtcatgg tgacagcccc tatgttgaat tcaaagaact
     3301 caccacatgg gacgccaatc acagaggtgg tgagacgtac agggttggcg taaccggtca
     3361 acatagaacc cggcgcatct tgcgatgccc ctgcacgccc caccataatt tgggcgtagc
     3421 aatgctcatg acacgaaaaa accgcatgag cgcggttatg ctctatattg aatttcagga
     3481 cgccaatccc ggcacccgct ttataaggtg cagagacagt gtaacgtccc gaaattgcag
     3541 aatcaatata ttgtgcagat ataacgattt tgttatgttt tgctgtatga actacactat
     3601 cgaatactac agtgaagagg tccgactgga ggtcgatcag cttccattga gtatgcgtgc
     3661 cagataccag catcttgttg aacgcatgaa ggtatacggc agcaatctcg gagaacctca
     3721 caccagcgcc tttggtgacg ggcttttcga actcagaatt aaaggcaacg atgggatcgc
     3781 gcgtgttttt tactgcactc tgacagggaa acgcatcatc atgctgcata gttttgtaaa
     3841 gaaaacgcag aaaacaccgc cagccgaacg caagaaagct gaaaccagaa tgaaggaggt
     3901 taagcatgac tggtaaacgc actctcccca ccatgacaca cgacgaaatg gcagccaaat
     3961 ggatggaaga cccggctttt aaagcagaat acgacgctat cgctgacgaa ttcgcactgc
     4021 ttgatgaaat gctggcagca cgcaaagaag ctggcttaac tcaggctgaa gttgccgagc
     4081 gaatgggaac aaaagcgacc gcgatcacca gaatggaaag taatctcgca tcaggtatca
     4141 gcggcccatc atttgccaca ctaaaaaaat tcgcccgcgc tactggaaaa aaactccaga
     4201 tccgcttcgt ttaaccacac cgcgccgtca ttctggcggc gcaccggata aatagtcatt
     4261 tcctcgcacg atgtcttagc caccggatat cccacaggtg agccgtgtag ttgaaggttt
     4321 ttacgtcaga ttcttttggg attggcttgc gtttatttct ggagcgtttc gttggaaggt
     4381 atttgcagtt ttcgcagatg atgtcggtga aacttcgtcg ctgtcgcctc attcatacct
     4441 cctgtcggta aatctgacac cctgaccaat agcccaggct gttgtgtact cgatcagact
     4501 tgccatgcgc ttcacgctca tctgcgcgct gctttcgcga atgttgacgc attctccttc
     4561 aaggccgggc aaaacatcag cttcctgttt tgttgccact gcatgaccgc tgatcaacaa
     4621 aaccttccat tgttctggtt ttaaccattt gccgcaccat tgaacctgac gagcgatatc
     4681 cgccagcatc gcgtgaaatt ttgcgttctg gtcaagattt cgcttgtagt cagtaatgcg
     4741 gatggtgact ggcttgtctt tatcgagagg agttgagagg atggcattta ttgcggcttg
     4801 ctgttgttgc ttagttcgga ggaagattgt ttgcttcatc gaaaattctt ctctttaatt
     4861 ccagcggctc tgatagcttt cattactgca attaccgttt tgtccttccc atcctcatgc
     4921 cccatcgcat aagcaccttc ttcaccatct ttccaaaagt cgtcattcga ttcgggccag
     4981 tcgatatcca gttcaatagc tgctcgcggt gcctgccata tcacccaggc aaactctttt
     5041 aattcatcgt ctcctgtgaa ctggcttttg tcttttgacc accagttttc aaactgtcgg
     5101 tagctatcgt tcacttccct ctcccccaaa taaaaaggcc tgcgattacc agcaggcctg
     5161 ctattagctc agtgatgtag atggtcattt aatactccgt cacgttttcc tgtcgccacg
     5221 cctcgtcata ttccgatttc ggcatattgg cgatgtagct atatggcgat cctgattcaa
     5281 gttgcaggaa ctggtgcgat tgctcgtcaa ggaacaacgg gacaccacct tcccaacctt
     5341 cgccgttacg ttgtttttca agcatcaaaa cagatgccgg agatgccagt agctgttcgt
     5401 ccttctctga catcttttca ccactctgaa ctctctgtaa cgctctctcg cgagccttgt
     5461 tacgccagat gatgaaaagg ttgtctgtca ggtctgttat cgctccagag ccttttacgt
     5521 ccattttccc ggttggtttt tcttcgctgt ctccttttcg cgagtgagta acgagaatga
     5581 cgtgggagtt tgttttgttt ttgaagtcgc aaatcgagtc aacaaacgcc ttctgcccgt
     5641 tatagtcatc gtcgcctatg ccacatttca tcaggctgtc gatgatgaat aactggatcc
     5701 cgtatcggcg gcgagcgtag tcgaatattt cgatcagcct gtcggctttc gccgttccag
     5761 tcaggccaaa cacccaaagt ctttcgtcat aaaatttaaa tgcagagtca atttccagca
     5821 ctggcggcat cttgcagcac gttgcctgac gggtaagtcg cttaaggaga ataccaggct
     5881 tcagctcaag tgacgcgatg cacgtcttca caccctgacg cattgcctca agtgccatat
     5941 gcccgacaac ctcggtgtta tgcgtcgcca catatcccct ggttacatac aggtggcgag
     6001 ggtgatcgac cataatacac aagcattctg cattgccgat tttttcaaca ttcctgacga
     6061 ataccccaca gccctcgaat cgatgcgcag ttaatctgcc attgagtcgt ggtgaacgga
     6121 tggcctccct gatttctctt gtcagtctga ttcttgcctc gtatgaatcc atcccatgcc
     6181 gcttatcgtc cttgtatgtg tatgtcacgc cagttttaac tcgcgtccgg catgacccgc
     6241 cgagtgagtt aaccagttga acaaccccat tgcgcagttc ttcacttgct gaggaaaaaa
     6301 caagcgttcc atccttctcg acatacccat ccgtttcaag cagaccacac agcatgccta
     6361 tacgcgttga tttatttgcg gaaaaaaaca cacgagggat gaatttgttt tttgctgtgc
     6421 accccattag tccataacct cgcagggtct ccatgagtgg atttacctga ccacgcgccg
     6481 ttgatatcag ccagtcctta ccatctccag agaagttgta atcaggcagt tcagccttca
     6541 tacgctcgat catgtatggc tcgacgtttg aaaacttcac gctcccattg ctaagactac
     6601 catccccgag aagggagccg ataacccatg ctaatggctc tgagtggtcg ccaaagtcac
     6661 cagttatttc aggaatccta acgccatttt tgtgcctctt cgtttcactc aaccgcttca
     6721 acccgaaggt atctatcacg cggcgtttct cgcctttcgt gaatccacga ctagtgacct
     6781 cccataggtg atcgcctgcg caatcaacat aacgaccatc ttcaaatgtg actcggtaaa
     6841 catctctaac accctgcggg aatatcccag tgacagttga cggattgccg tctactgacg
     6901 ccacctgatc gccaattttt acattcccgt gagtagtcca tgtcccatca gctaaaagaa
     6961 taggctcatt cagtgggcat gcctttccgt gaccgttcac accattgacc agcgtcaact
     7021 ctgcctcacg gaactggaat ttatctgcca gagattccca cggtggatta aacagatact
     7081 gctgcttgcc gtagaaagcg ttgatagtgt cctggtaaaa ctctcgcgca ctgtagagtt
     7141 cttcaggatc gaagtaggat gccgtgccga tgtactgcca gatttcatcc tcggtaacac
     7201 cgttcatcag gcattcgttg atgtctttgt acggcagagt aacaagacgg caa
//
LOCUS       DAJTYI010000170         7216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYI010000170 DAJTYI010000000
VERSION     DAJTYI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..661)
                     /gene="chbR"
                     /locus_tag="OO564_003623"
     CDS             complement(<1..661)
                     /gene="chbR"
                     /locus_tag="OO564_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6654580.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQ"
     gene            complement(669..1019)
                     /gene="chbA"
                     /locus_tag="OO564_003624"
                     /pseudo
     CDS             complement(669..1019)
                     /gene="chbA"
                     /locus_tag="OO564_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1078..2426)
                     /gene="chbC"
                     /locus_tag="OO564_003625"
                     /pseudo
     CDS             complement(1078..2426)
                     /gene="chbC"
                     /locus_tag="OO564_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2511..2831)
                     /gene="chbB"
                     /locus_tag="OO564_003626"
     CDS             complement(2511..2831)
                     /gene="chbB"
                     /locus_tag="OO564_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6654581.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3130..3468)
                     /gene="osmE"
                     /locus_tag="OO564_003627"
     CDS             complement(3130..3468)
                     /gene="osmE"
                     /locus_tag="OO564_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6654582.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3670..4497
                     /gene="nadE"
                     /locus_tag="OO564_003628"
     CDS             3670..4497
                     /gene="nadE"
                     /locus_tag="OO564_003628"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6654583.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4727..5614
                     /gene="cho"
                     /locus_tag="OO564_003629"
     CDS             4727..5614
                     /gene="cho"
                     /locus_tag="OO564_003629"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6654584.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5574..6149)
                     /gene="ves"
                     /locus_tag="OO564_003630"
     CDS             complement(5574..6149)
                     /gene="ves"
                     /locus_tag="OO564_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6654585.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6352..6837)
                     /gene="spy"
                     /locus_tag="OO564_003631"
     CDS             complement(6352..6837)
                     /gene="spy"
                     /locus_tag="OO564_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6654586.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1906 a         1761 c         1661 g         1888 t
ORIGIN      
        1 tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt
       61 tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca
      121 tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt
      181 aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg
      241 tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg
      301 tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa
      361 aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca taaaaacttt
      421 gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac
      481 cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct
      541 gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag tttttgccat
      601 taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca
      661 tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat cagttcacgc
      721 gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact cactttcatc
      781 ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat
      841 gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg cttcgcctgt
      901 ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag ccccatcacc
      961 acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag atccatcata
     1021 catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat taaaatttca
     1081 ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg ctttgttagc
     1141 caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga gtgcgaccag
     1201 caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca
     1261 cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga gggtgattgc
     1321 cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg
     1381 cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc
     1441 cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca ggcctaaagt
     1501 cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa
     1561 ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt
     1621 ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg
     1681 aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc
     1741 caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca
     1801 ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa
     1861 tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt
     1921 gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat
     1981 gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga cactatatgg
     2041 ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg
     2101 ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta ttcctaatgt
     2161 tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa tggttgaggc
     2221 gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag aaaaacgttg
     2281 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
     2341 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
     2401 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
     2461 tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
     2521 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
     2581 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
     2641 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
     2701 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
     2761 cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag ataaatgtgt
     2821 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
     2881 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
     2941 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc
     3001 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
     3061 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
     3121 acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
     3181 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
     3241 acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc tcacttcaga
     3301 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
     3361 caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac ccgccagcat
     3421 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
     3481 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac
     3541 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
     3601 tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
     3661 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
     3721 aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct gcaaacttat
     3781 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga
     3841 aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga atcactgcaa
     3901 tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg ccaggatgcc
     3961 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggct
     4021 agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa
     4081 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
     4141 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
     4201 ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca gttactgacg
     4261 gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct tgaagatgat
     4321 cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
     4381 ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
     4441 accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa aaagtaataa
     4501 tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
     4561 gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg aataggcctg
     4621 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc
     4681 ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
     4741 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttaca
     4801 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
     4861 caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt
     4921 acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgcac
     4981 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
     5041 gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg
     5101 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
     5161 gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg aacaaaaact
     5221 ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc
     5281 gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg
     5341 cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc
     5401 actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
     5461 gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga
     5521 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
     5581 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc
     5641 ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
     5701 agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag
     5761 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgc
     5821 gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt taccacctgg
     5881 tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt
     5941 tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa
     6001 gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
     6061 gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc
     6121 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
     6181 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca
     6241 ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
     6301 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc
     6361 agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc
     6421 attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct gggtttccat
     6481 gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt
     6541 tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg
     6601 cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct gtttctgcgc
     6661 gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg
     6721 gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg catgggccag
     6781 gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt
     6841 ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc gtgttgacga
     6901 gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt
     6961 aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag agtgatgaga
     7021 gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga tgactggtga
     7081 taaataaagc aaataaccag gattaatctg tattaattta taagaaagca acttaatacc
     7141 cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa tcccgcgcgt
     7201 aatcccaacg ctacca
//
LOCUS       DAJTYI010000171         7053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYI010000171 DAJTYI010000000
VERSION     DAJTYI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..899
                     /locus_tag="OO564_003632"
     CDS             <1..899
                     /locus_tag="OO564_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6654587.1"
                     /translation="IFEICDDVTVFRDGQFIAEREVASLTEDSLIEMMVGRKLEDQYP
                     HLDKAPGDIRLKVDNLCGPGVNDVSFTLRKGEILGVSGLMGAGRTELMKVLYGALPRT
                     SGYVTLDGHEVVTRSPQDDLANGIVYISEDRKRDGLVLGMSVKENMSLTALRYFSRAG
                     GSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRPKVLILDE
                     PTRGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLCGEFTR
                     EQATQEVLMAAAVGKLNRVNQE"
     gene            904..1863
                     /gene="rbsC"
                     /locus_tag="OO564_003633"
     CDS             904..1863
                     /gene="rbsC"
                     /locus_tag="OO564_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6654588.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1888..2765
                     /gene="rbsB"
                     /locus_tag="OO564_003634"
                     /pseudo
     CDS             1888..2765
                     /gene="rbsB"
                     /locus_tag="OO564_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2891..3820
                     /gene="rbsK"
                     /locus_tag="OO564_003635"
     CDS             2891..3820
                     /gene="rbsK"
                     /locus_tag="OO564_003635"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6654589.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3824..4816
                     /gene="rbsR"
                     /locus_tag="OO564_003636"
     CDS             3824..4816
                     /gene="rbsR"
                     /locus_tag="OO564_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6654590.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4782..6209)
                     /gene="mdtD"
                     /locus_tag="OO564_003637"
     CDS             complement(4782..6209)
                     /gene="mdtD"
                     /locus_tag="OO564_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6654591.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6232..6924)
                     /locus_tag="OO564_003638"
     CDS             complement(6232..6924)
                     /locus_tag="OO564_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6654592.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1775 a         1702 c         1871 g         1705 t
ORIGIN      
        1 aaatcttcga gatttgcgat gacgttaccg tttttcgtga tgggcaattt attgctgagc
       61 gcgaagtggc atcactgacc gaagattcgc tgattgagat gatggtgggc cgcaagctgg
      121 aagatcaata tccacacctg gacaaagcgc caggagatat ccgcctgaaa gtcgataatc
      181 tctgcggacc tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg
      241 tctctggttt gatgggcgca ggtcgtaccg aactgatgaa agtgctctac ggcgcactgc
      301 cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg
      361 atgacctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgc
      421 tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg
      481 gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgatttcatt cgtctgttta
      541 atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa
      601 aagtggcgat tgcccgcggt ctgatgacac gccccaaagt attgatcctt gatgagccta
      661 cccgtggcgt agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag
      721 ccgatggctt gagtatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg
      781 atcgcatcat cgtcatgcat gaagggcatc tctgcgggga atttactcgt gagcaggcca
      841 cccaggaagt gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa
      901 aaaatgacaa cccagactgt ctctggtcgc cgttatttca cgaaagcgtg gctgatggag
      961 cagaaatcgc ttatcgctct gctggtgctg atcgcgattg tctcgacgtt aagcccgaac
     1021 tttttcacca tcaataactt attcaatatt ctccagcaaa cttcggtgaa cgccattatg
     1081 gcggtcggga tgacgctggt gatcctgacg tcgggcatag acttgtcggt aggttctctg
     1141 ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg
     1201 gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg cggtaaccgg ggtgattgta
     1261 gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta tgatgctttt actgcgcggc
     1321 gtgaccatgg tttataccaa cggtagccca gtgaataccg gctttactga gaacgccgat
     1381 ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg
     1441 gggattgtct tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc
     1501 tacgcgctgg gcggcaacga agcggcaacg cgtctttctg gtatcaacgt caataaaatc
     1561 atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggatcattga agtggcgcgt
     1621 ctctcctctg cacaacccac ggcggggact ggctatgagc tggatgctat tgctgcggtg
     1681 gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc
     1741 gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc ctcctattac
     1801 cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa caaaaagcag
     1861 taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac cctggtttcc
     1921 gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg
     1981 gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc gcagaaagag
     2041 gcggataaac ttggctataa cctggtggtg ctggactccc agaacaaccc ggcgaaagag
     2101 ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc
     2161 gactccgacg cagtgggtaa tgctgtgaag atggctaacc aggcgaacat tccggttatc
     2221 actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcatc tgataacgta
     2281 ctgggcggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt
     2341 atcgagctgc aaggcattgc cggtacatcc gcagcccgtg aacgtggcga aggcttccag
     2401 caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agcagatttt gatcgcacca
     2461 aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag gctgtattcg
     2521 cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc cggtaaatcg gatgtgatgg
     2581 tcgtcggatt tgacggtaca ccggatagcg aaaaagcggt gaatgatggc aaactagcag
     2641 cgactatcgc tcagctaccc gatcagattg gcgcgaaagg cgttgaaacc gcagataaag
     2701 tgctgaaagg cgagaaagtt caggctaagt atccggttga tctgaaactg gttgttaagc
     2761 agtagtttta atcaggttgt atgacctgat ggtgacataa atacgtcatc gacagatgaa
     2821 cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac acggtggcgc attttatgga
     2881 catcccgaat atgcaaaacg caggcagcct cgttgttctt ggcagcatta atgctgacca
     2941 cattcttaat cttcaatctt ttcctactcc aggcgaaacc gtaaccggta accactatca
     3001 ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa
     3061 tatcgcgttt attgcctgta ctggcgatga cagcattggc gagagcgttc gccagctgct
     3121 cgccactgat aacatcgata ttactccggt cagcgtgatc aaaggcgaat caacaggtgt
     3181 ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa
     3241 tgctgctctt tccccatcgc tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc
     3301 gttattaatg cagctggaat caccactcga aagtgtgatg gcagcggcga aaatcgccca
     3361 tcaaaataag actatcgttg cgcttaaccc ggctccggct cgcgaacttc ctgacgaact
     3421 gctggcgctg gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat
     3481 tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat
     3541 ccgtactgta ctgattactt taggaagtcg tggtgtatgg gctagcgtga atggtgaagg
     3601 tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc attgctgccg gagatacctt
     3661 taacggcgcg ttaatcacgg cgttgctgga agaaaaacca ttgccagagg cgattcggtt
     3721 tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg
     3781 gcgtgaagag atcgacgcat ttttagacag gcagaggtga cgcttggcta caatgaaaga
     3841 tgttgcccgt ttggcgggcg tttctacctc aacagtttct cacgttatca ataaagatcg
     3901 cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg attaaagaac ttaattacgc
     3961 gccatcagct ctggcgcgta gcctcaaact caatcaaaca cataccattg gcatgttgat
     4021 cactgccagt accaatcctt tctattcaga actggtgcgt ggcgttgaac gcagctgctt
     4081 cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg
     4141 caatctggaa acgctgatgc aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac
     4201 gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga
     4261 ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc tgggcggaga
     4321 cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta ttaccggtcc
     4381 gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc
     4441 gggtctcaac attcctgatg gctatgaagt cactggtgat tttgaattta acggcgggtt
     4501 tgacgccatg cgccaactgt tatcacatcc gctgcgtcct caggccgtct ttaccggaaa
     4561 tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac aggttccgca
     4621 ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga cgccaccatt
     4681 aaccactatc caccaaccga aagatgaact gggggagctg gcgattgatg tactcatcca
     4741 tcggataacc cagccgaccc ttcagcaaca acgattacaa cttactccga ttctgatgga
     4801 acgcggttcg gcatagattt acgctgtctt ttgatcaaat tattaccatc ggttgttttc
     4861 agaagcatga acattgctgc tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag
     4921 tggaattgtt cgacagtcgt tgtgccttcc atcccttcat aaatgcgaag gacggccgca
     4981 cttacagcaa cgcctaaact aatcgacagt tgctgcgtga ccgccagaac attgttaccg
     5041 ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg tattcatcgc ggtaaactgc
     5101 gtcgacatag ccatccctaa tataaacaac ggcaagatca gcatccatat cgccattgcc
     5161 ggtgattgca aagagaactg agcgatcatt agcccaataa tcaccgtgat ccccactaat
     5221 gtatggcgat agcccagacg acgtaagact tgggtaacca tcgattttgc aataatggaa
     5281 cctaacgctg tcggtgccat catacagccg gcaataaacg cctgataacc aaatcctacc
     5341 tgtaacatca atggcataag gaacggtaca cagccggtcc ccagacgggt tgcaatattg
     5401 cctacgatac cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat taatgggttt
     5461 ggcgtacgtc gcgcatggag aatatagaga agcagtaacc cgatgctggt gacaattacc
     5521 gtcaaggcaa tccagctggc gacaatcttt tccccgaata gctctattcc gcttgaaaag
     5581 agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg
     5641 gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg caatacctat ggggatatta
     5701 attaaaaata tccagtgcca ggttgcccag gtgaccagca cgccgccaag aacggggcct
     5761 aaaattggcc ccaccagacc cggcatggcg acaaaattca ataccggaag aagttcatta
     5821 cgaggataag cacgcagtaa ggccagccga gtaacaggca tcatcattgc gtcgcctatc
     5881 ccttgaataa cccggaagac aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga
     5941 gaacccaacg tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg
     6001 gctagccatc cgcttaccgg aataagcatc gccaccgtca gcgtataact gatgatggct
     6061 gattgcatcg cgagaggaga acgattaagg ctatgagcga ttgcgggtaa ggcggtattc
     6121 agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg ccgcgatcca cggcaaaccc
     6181 gacatactac gcttcttttt atcgctcatt caatgtcctg ttatcgggtt atcacttatc
     6241 aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc tttggataat
     6301 cgcatcgaca atcgcctggt gcagatccag ctttatcact gtgtcgctgg taattgacgt
     6361 gaagtaagtg tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa acggattggc
     6421 gctcatttca tagatatgct catgccaggc tatatcgact tcgatccagc gttcacggcg
     6481 aaagttctct tttaatgccg ccatttcggc cattaacgta ttgagatgcg ccttctgttc
     6541 cgcggtgcca accgttgctg ccagtaggca ggcttgcggc tccagacaga tacgcataac
     6601 cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc atccaccaag taagcaattc
     6661 ctgatcaaga aaattccagt tcgattgtgg catgacccga gtaccaattc gcggtcgcgg
     6721 taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac
     6781 tccaaattgc tcgcccagct caatctcacc gggcaaaatg gtgccgggtt catattcacc
     6841 ttttaagatc cgttgcgcca gcttctcagc cagaacatac gaaaggtttt tctgtgcagc
     6901 taactgttgt gcgcttaaag gcattactta tcttcctttt tctttttatt cctccttagt
     6961 atgccaccag gaagtgtgat tacggttgca aaaacggcaa attgattgtt ttatggcaca
     7021 ttaacggggc ttttgctgaa aaaatgcgcg gtc
//
LOCUS       DAJTYI010000172         7035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYI010000172 DAJTYI010000000
VERSION     DAJTYI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..684)
                     /gene="envY"
                     /locus_tag="OO564_003639"
     CDS             complement(<1..684)
                     /gene="envY"
                     /locus_tag="OO564_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6654593.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYF"
     gene            complement(867..1757)
                     /locus_tag="OO564_003640"
     CDS             complement(867..1757)
                     /locus_tag="OO564_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6654594.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(1758..4736)
                     /gene="nfrA"
                     /locus_tag="OO564_003641"
     CDS             complement(1758..4736)
                     /gene="nfrA"
                     /locus_tag="OO564_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6654595.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(4723..6960)
                     /gene="nrfB"
                     /locus_tag="OO564_003642"
     CDS             complement(4723..6960)
                     /gene="nrfB"
                     /locus_tag="OO564_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6654596.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1633 a         1834 c         1856 g         1712 t
ORIGIN      
        1 aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat ttttgttatc
       61 catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct ggctatagct
      121 ggtattttcg ttttttaatt tctttttgag caggctgggg cttaaacata gcgaactggc
      181 gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc agacgctgtc
      241 gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt gctcaagaaa
      301 gttagacaat acggtaaaaa gtaatgcgcg cgtcaactcc gcttcgcagg gtttgctggg
      361 ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc tatgacaagc
      421 gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaatttct tatttaaaaa
      481 caacagataa tagttgatga tgttacggtt gatgtgggtg attaatacgt gatttaacgt
      541 tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt tcggaagggt
      601 aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga tcaccacgca
      661 aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact ccgctcaaag
      721 gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt ctttctttag
      781 catgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag gtaagccagg
      841 tcgtacccga cttacctgga ggggatttaa tactcgagaa tgccgtgcgc gacgggcaaa
      901 tagcgcagag agaaatagag catgtcttta ccgcaagagg aacgtttcgc taacagcgag
      961 gcaatttctg cgtccggttt cggtttcgct gtaaaggttt tgcctttgcc ggcgacaaac
     1021 agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct ggcctgtaga
     1081 taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg tacccagaca
     1141 ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatcggg cgacatgttt
     1201 ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat cagccgctgc
     1261 gcgttgttta accatgttag caaaggttga cgggcggctt cgctgcgcca gttcaggtcg
     1321 tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc gctccataat
     1381 ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag cgctgcagac
     1441 gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat cagcttcagg
     1501 ccagtctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc tggctgggta
     1561 aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg aaaacgtaac
     1621 tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt ttgtggttgc
     1681 cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt cagcaatacg
     1741 aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt gttgcgctct
     1801 ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag actgactttg
     1861 tgcggccagg cgtcgtagtg cgtctcgccg gtccaaatat tccagcggac cccgacaccg
     1921 ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac gtgagcgtaa
     1981 ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata atctgccgtc
     2041 cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt ttgggcaaac
     2101 cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa gaatgaggcg
     2161 ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa ctgctgttcg
     2221 acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt gccggacatc
     2281 ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac gcggctataa
     2341 actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc ggcttccagt
     2401 tgtccgtagc tacgatagct tttcccaggc gctgcaccgc cgacattatt gttagcggta
     2461 ctcattgcgc cggaacgcaa gccgatggaa gaatcgaaac tgaacgtcca gcggcgaccg
     2521 acctcctcat gcaaacggcg gaaattgaag cgttgctgat tttgctctgg ggtcagtggg
     2581 gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg ctgcgtcgca
     2641 ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc cggatcgtcc
     2701 ggaagccctt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc accgctatcc
     2761 cacaaggcgt aaccaagcgc tgcctgggtg ttgctattat tcggttccag ttccagttcc
     2821 agcgcggcgc gcaaatcact caccgcggcc ggaacattat gacgttggcg ataaattgtc
     2881 gcccgcgcaa cgtaagcgtt ggcagaaggg gcaatattga ttgagcgcgt gagatcgttc
     2941 agtgcgagtt ctggctgagc tgggatataa cgttgcgcat gcagccacca gtattgagca
     3001 ttgtttccca gctcacgttg ttctgcctgt tgaagccagc gatcgcgagc cgcgccattt
     3061 cccgctgcct gagcggtgtt ggcggcggcg agcagatcct cattgctcat gtcgtgaaga
     3121 ctgattttct gccaggccgc cagtgcggtg gcgtagtcct caacctgata cgcctgatag
     3181 gctaccgcac gatgttgcca ggcgctcggt tgtcgttgtt cggcctgaag ccatgcatac
     3241 aacgccacac cgggtagcgt gtcccgataa cactttgcca gacggttcca ggcggcagca
     3301 tcgtaggaag gcgacatatc gcccagcaag cgaactattg ccgggcaatt atctgcaata
     3361 cccggcaact gactttgcca ctgacgttgc tccgccagcg gtaagggttt cgataaaatc
     3421 gccaccttcg ccggcgttgc taggtaagga tgactttcca gcagagacgc cagtcgcgcc
     3481 attaaagtct ggctgacacg cgcatcgccc tggaaaggat agcgttgcag caataaatcg
     3541 gcagcttcgc gtgactgctc gttctgcatc agttgccagg tcagttgatc caggcgggga
     3601 agatttgtcg gttcttgctg atacagcaat cgtgccagac gcagagattc agccttgtta
     3661 cgggtcgcca cgctgacagc ataacgctcc tcaagcattt cattggcggg gagggtggcg
     3721 agcagttttt gcgctgcgtc gtactgaccc tcttttaaca gcaccggtag cgtcgcgcca
     3781 acaacatact ggcggttatc ggcaaactgc accgtataat tcgccaatgc ctgaacgggg
     3841 ttggcgctgt atttagataa cagatagagc caacttttct cttgtgcgcc cgtggtaaac
     3901 agcggcttat tttcaatgag ataatgctgg aggcgtgctt tttcgccacg ataagccagc
     3961 gcggtcgcgt aagtaatata tgactgagga tcggtgaaga tcccctgtga ttgcagtgcc
     4021 aggatccgat cgtccagctg cccggcaaga agcacgtcaa accactgacg gcgttctgcc
     4081 gcgcttaatg tgttctgctg gcgtgcttca ttgtatagcg tatctgcctg ggaccattgt
     4141 ttcaggtaga ttgcccgttg cagcagatcg gttcgcagcg tttttccttc cggcgatgca
     4201 gcaaacgtcg catcgttcag ttgcgctctg gcgacaggta attgtgccag ccgcagggca
     4261 ttctgcccga cttcactgcg acaacgcagg gtcggcgcag cgtcgcccgc tttttgctgg
     4321 gcaagcagtt cttcaacagt cgtaacgctt ttcacttcaa ctggaatagc cgccagactg
     4381 cgctcaagtc gggcatctcc tgggtgacgt ttcagttgat cctcaagcaa cagccgcgcc
     4441 cggtcatcat gaccaaaatg acgataggct tccgcaaggt ataaagtcag cggaatatta
     4501 tccggcacct gttggtgtat atattcaaat tcgcggatgg cggttgcttc gtcgttattt
     4561 ttctgtgcct tcagcgcctt atcgagacgg ggataaataa caaaatggcg ataatcgctc
     4621 agccccagct cttctgcgct ggtgccgata ttgtctgcga gtgcgctggt actcaataaa
     4681 gacgtcagca gtaaaccaga ccatccgatg acgcgattaa ggttattctc cttcattttc
     4741 ggactccagt tgcgcaacct gttctgtgtt taaacctgct ttgagtaata gtgattgcat
     4801 cgaaacttgt aattcgcgtt gaattgtcag gacgcgatcc aacgtttcct ggctgataac
     4861 gccttcggtg accaaaaact tgccgagcgg cagagaactg cgttcatggc gcaataacaa
     4921 cacgttaatt gctgaacgat taatatgacc gagcgtggtc agtatttcgg cgaacaggaa
     4981 ctgatgcggc acatattgcc gccagatttc accggtctgc tgttccgtga gccactgatg
     5041 ctgaaccgca ttgtacaaca ttgcccgcgg atcgtgaccg cgtcggcgtg cataccagtg
     5101 gcgtaaccct gtgacaattt gtccccgcag aacaatgacg taacgcactt tgcgtccgac
     5161 tttacgcgtc agggccgcca gcgaaaccgg gtcaatacca tcttcactgc cgacaattaa
     5221 ctcatcattt tccagacgca gcggcagtac tgcataatgc agcgctacgg aggctggcat
     5281 ttcggcaatc agcgaggacg ggatctgcca ggcatcgatg gattcccacg ccacgccgtt
     5341 ttgctctgcc tgcgcctgtg ccagctgctc ggcgctaatc agcccctgca tcagcattga
     5401 accgcccagg cgtagacctt cgacgcgatt acgcagtgct gtatcgagtt gttcttcagt
     5461 gatgacctga ttttccagca gaatttgacc taacgggcgc aacgagcggg tatcgccagt
     5521 gacgctgggg aagtcatgcg ttgttttatc ccacgccacg cgacgcggat cgccgtgctg
     5581 aagaacctgt ttcagcacgc gccagttagc cataaagtta atcaggttgc cccagaaaag
     5641 acgcaggacg gaaagcaggc cctgcgtgag gccgtagtag ccagtgacga aaatcactcg
     5701 ctgcacgatg cggttaacca tcaagccaaa gtttagccac agcagggtca ttaaccatgc
     5761 gctgccgcta aaaatagaaa ggaaatgcca ggcatcgggc cacaaacttt catacgccag
     5821 caacagcaaa agctggatca tcaccagcat cgcgaggaag ctgacaaagt tactgattgc
     5881 ccctttgcgg tcgcgccaga gaaagtagtt cagcgtcagg ctggaggtcc atttatgggt
     5941 tttaaagccc tggaaaacaa tgccgatgat ccagcgagat ttttgtcgaa ccgcagtcga
     6001 aaaggtatcg gggaaatatt cgcgcacgca gatcatgttt gacgtccgtg cgtgctgtaa
     6061 aaatttacgc tgctcgcgtt ctttggcttc gtccaccacc ggaaaacgga caaaaatttc
     6121 cgtcatacct ttttctttca ggcggaagcc aatgtcgtaa tcttcagtaa gactctgcac
     6181 gtcgaaagca ataccgttac cgtcagctaa cagtgcggtc acggcgcggc ggctgaaaca
     6241 ggtgccgacg cctgcgctgg gcacttgtcc ggcgagggct tcacgcaccg gaacatcttt
     6301 gccatgcagt tctgaaaact catcaatgta agtcatgctg gtgaagtgcg tccattcgcg
     6361 ttcaaacgga tacactggga tctgaatcag atctttacgc tcgaccagat agttgaacag
     6421 acgcaattcc atcggtgaaa tcacatcttc ggcgccatgc agaataaaac cagcaaaagc
     6481 gaaattggcg ctacgttcaa actgggtgat ggcgtccagc acgttgttca gacagtcggc
     6541 tttgctggtg ggaccaggac gcgcgcagac taccttatgc acgttcggaa agcgagcgca
     6601 cacttcgtca acatcacgtt gagtatcggg gtcgttgggg taggtgccaa caaagatatg
     6661 atagttttcg tagtcgagcg tggtcgccgc cagctcggcc atattgctga tgacgcccgt
     6721 ttcattccac gccggaacca taatcgctaa cggtttttca tctggtttat acagttcgcg
     6781 gtaactcatt cgcgggtagc ggcgataaac actcaacttg cgtttaatgc ggcgtaccca
     6841 gtagacgaca tcaataaaaa aatcgtccag cccgctgatg aacatgatga ccgctaacgt
     6901 tatcgcgatt acttttaagc cgtagagcca ggtagcaaaa acatcaagaa gccagtccac
     6961 acaaaaacct tacattaacg ctggttatgt ttagggtggc gtatattaag gttttttatg
     7021 aattgtgaca gcttt
//
LOCUS       DAJTYI010000173         6955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYI010000173 DAJTYI010000000
VERSION     DAJTYI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..919
                     /locus_tag="OO564_003643"
     CDS             <1..919
                     /locus_tag="OO564_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6654597.1"
                     /translation="EAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYV
                     YFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLL
                     TPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSED
                     NLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQN
                     VSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQE
                     GDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            962..1465
                     /gene="yeaK"
                     /locus_tag="OO564_003644"
     CDS             962..1465
                     /gene="yeaK"
                     /locus_tag="OO564_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6654598.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1466..1570)
                     /locus_tag="OO564_003645"
     CDS             complement(1466..1570)
                     /locus_tag="OO564_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654599.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1740..2186
                     /locus_tag="OO564_003646"
     CDS             1740..2186
                     /locus_tag="OO564_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6654600.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2143..2964)
                     /gene="nimR"
                     /locus_tag="OO564_003647"
     CDS             complement(2143..2964)
                     /gene="nimR"
                     /locus_tag="OO564_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6654601.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3061..4242
                     /gene="nimT"
                     /locus_tag="OO564_003648"
                     /pseudo
     CDS             3061..4242
                     /gene="nimT"
                     /locus_tag="OO564_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4297..4644
                     /locus_tag="OO564_003649"
     CDS             4297..4644
                     /locus_tag="OO564_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6654602.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4666..4920)
                     /gene="yoaF"
                     /locus_tag="OO564_003650"
     CDS             complement(4666..4920)
                     /gene="yoaF"
                     /locus_tag="OO564_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6654603.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5103..6128
                     /gene="dgcP"
                     /locus_tag="OO564_003651"
     CDS             5103..6128
                     /gene="dgcP"
                     /locus_tag="OO564_003651"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6654604.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6161..6259
                     /locus_tag="OO564_003652"
     CDS             6161..6259
                     /locus_tag="OO564_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6654605.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6262..6336
                     /gene="yoaJ"
                     /locus_tag="OO564_003653"
     CDS             6262..6336
                     /gene="yoaJ"
                     /locus_tag="OO564_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6654606.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6388..6819)
                     /locus_tag="OO564_003654"
     CDS             complement(6388..6819)
                     /locus_tag="OO564_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654607.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6813..>6955)
                     /locus_tag="OO564_003655"
     CDS             complement(6813..>6955)
                     /locus_tag="OO564_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6654608.1"
                     /translation="EAGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKT
                     SC"
BASE COUNT         1681 a         1674 c         1693 g         1907 t
ORIGIN      
        1 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
       61 tgattacggt tcaggtcttg ggatagccga agagaaattt cactattatc tcgatctcaa
      121 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
      181 gtccttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
      241 cgggcgtacc gtattgtcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
      301 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
      361 caaaaacaat ttgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
      421 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
      481 taatctgttc cagtatgtca gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt
      541 atccattgat attctttact ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt
      601 gacggcgtta attttgctga atatggtccg gatgcacttc cgtttatacc aaaatgtgtc
      661 gcgagaaaat attagtgatg cgatgactgg actgtataat cgcaaaattt taacccctga
      721 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgcaat
      781 tgacatggac aagttaaagc aaataaatga caacctcggt catcaggagg gggatttagc
      841 gattacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
      901 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga
      961 aatgactgaa atggctaaag gaagcgtgac gcatcagcag ttaatcgctt tattatcaca
     1021 agaaggtgct aacttccgcg tcgttatcca tgaagcggta ggtaaatgtg aagcggtgtc
     1081 agaaattcgt ggcaccgctc taggccaggg tgctaaggca ctggtatgta aagtcaaagg
     1141 caatggcgta aatcaacatg ttctggcgat cctcgcagcc gatcaacagg ccgatctgag
     1201 ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagttga
     1261 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     1321 actggttgcc gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat
     1381 gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc aaccagaact
     1441 ggtcaatttc cgccgcactg cgtagctagc ctatccgttc aataagcaga acggacaaaa
     1501 ccagcacaac cgcaatagca aaaaacgatg atgtgataat cagtgtctcg acgaacattt
     1561 gatcgttcat agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa
     1621 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     1681 tccttcctgc agcaagcgta aagtaagcag gcttattatt ttttggcaag gaaaccacga
     1741 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     1801 acaccactgt cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca
     1861 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     1921 gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc
     1981 tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg ctgggtgggc
     2041 gcggcgtgac gttaatgggc agccagccgc aactggttgc tgggctgctg gtgggcactg
     2101 ttttaggcgt agcgctgttt cgcggcgtac cggttggccc gcttattgcc gcgggtctgg
     2161 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     2221 taggcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     2281 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     2341 ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcagtcggg caagattgcg
     2401 ttcactcatc gcaaaaaagc cagcccattg ccccaatgac ccccactcga cgggcccttt
     2461 cgccatcatc tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg
     2521 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     2581 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa attaattccc ggcataaggg
     2641 cgatattttc aatgtgcaac atgttgtcgg cattgtcact gcgctgggtt cgataaacag
     2701 aaaacagagt tcagcgttag ctgtcacttg attgctatgc ttcaccccgc ccggtatcca
     2761 aaccgcatat tgtggcggca ccatccacaa agcattttcc accgtacagg taattgcacc
     2821 atgtaatgct agtatcagct gtcctttgcg gtgctgatgt acaggactgg ttaactcatc
     2881 cgttccggcg tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc
     2941 attgagattc aacctgtgca tcattttgtc cgaacttagc gataatttgt cattttagct
     3001 tgattcaaca taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat
     3061 atgacctgtt caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg
     3121 atgattgcca caacattacg cgtcaccttt accggcgcag cgccgttact ggatacgatt
     3181 cgttccgctt actcgctgac gacagcgcaa accggcttat taaccaccct gccattattg
     3241 gcctttgcgc taatctcacc tctggctgcc ccggtggcgc gacgttttgg tatggaacgt
     3301 agcctgtttg ccgcgttact tttgatctgt gcaggtatcg caattcgttc tctcccttcg
     3361 ccttacttat tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc
     3421 ttactgccag gattaattaa acgcgatttc cctcattccg tcgccagact taccggcgca
     3481 tattccctga caatgggagc tgcagcggca ctgggatcgg ctatggtcgt gccgctggct
     3541 ttgaacggtt ttggctggca aggcgcgttg ttcatgctga tgtgttttcc tctgctggct
     3601 ctttttttat ggctgccaca gtggcgaagt cagcaacatg caaatttgag tacctcacgc
     3661 gccttacata ctcggggtat ctggcgttcg ccacttgcct agcaggtaac attgtttctt
     3721 gggatcaact cactggtcta ttacgtgatt attggctggc ttccggcgat cctcatcagt
     3781 cacggctata gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca
     3841 gctcccggtt tgctgatccc acttttctta catcatgtga aagaccagcg tggtattgca
     3901 gcgttcgttg ctttgatgtg cgcagtgggc gcggttgggc tctgctttat gccagcgcac
     3961 gcgatcacct ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg
     4021 acgttcattg gtcttcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca
     4081 caatccgtcg ggtatttgct ggcagcctgt gggccgccgc taatgggtaa aatacacgat
     4141 gctaacggta actggtccgt accactgatg ggtgttgcaa tactttcact actgatggcg
     4201 attttcggac tttgcgccgg gagagacaaa gaaattcgct aatatccggt gctatagtga
     4261 cgtgacaaat cttgcgtgaa agggagaaca aacacgatga atattcagtg caaacgcgtt
     4321 tatgatccgg ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccgcgc
     4381 ggtatcaaaa aaaccgattt agcccttgat gagtgggata aagaaatcac gccgtcaacg
     4441 gaactgcgca aagcctttca cggcgaagtc gtcgattttg caacctttcg cgagcaatat
     4501 cgcgcagaac tggcgcaaca cgagcaagaa ggaaagcggc tggcggacat cgccaaaaaa
     4561 cagccgctga ccctgctcta cgcagcaaaa aacaccacgc agaaccatgc gctggtgctg
     4621 gccgactggc tacgtagctt gtgattttag tacggcatcc ggcggttatt tttcaccagc
     4681 cggatggtcc cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca
     4741 attgttgctg accccttgcg ctgtctgcac tggaatgagc gtcccgccct tctgctggca
     4801 atagaccgac gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga
     4861 aggggttgaa caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat
     4921 ttattgatct cacatattta tccaagctta gagtatcgcg gtatcgtttt gttttgcagc
     4981 actattttta ttacattcac tcaaaacata ttacgtcttg tttcatcttt gttgatgatg
     5041 ttttatcatg cctgcaaaga ttaaataatc agcacttacc cgccgtatcc tggagttgtt
     5101 ccgtgtcaga tcagattatc gcccgcgtct cgcaatccct tgccaaagaa cagtcactgg
     5161 aaagtctggt ccgacagctt ctggagatgc tggaaatggt cactgatatg gaatcaacct
     5221 acctgaccaa agtggatgtc gaagcgcgcc tgcaacatat tatgtttgcc cgtaacagcc
     5281 agaaaatgca catcccggag aattttaccg tctcgtggga ttactcgtta tgcaaacgcg
     5341 ccattgatga aaactgcttt ttcagcgatg aagtccctga ccgttggggc gactgtattg
     5401 cggcacgcaa tcttggcatc accacatttc tgagcacgcc aattcactta ccggatggat
     5461 cattctatgg cacgctttgc gccgccagca gtgagaagcg ccagtggagt gaacgcgcgg
     5521 aacaggtttt acagttattc gccggactga ttgcacaata tattcaaaaa gaggcactgg
     5581 ttgaacagct gcacgaagcc aatgctgcgc tgattgcgca atcgtatacc gactcgttaa
     5641 ccgggctacc gaatcggcgg gcgatttttg aaaatctgac gacgctgttt tccctcgccc
     5701 ggcatcttaa ccataagata atgatcgcgt ttatcgatct ggataacttc aaattaatca
     5761 atgatcgttt tggtcataat agtggcgatc tgtttctcat tcaggttggc gagcgcctta
     5821 atacgctcca gcaaaatggc gaagttattg gtcgtctcgg tggtgatgag tttttggttg
     5881 tttcactaaa caacgagaat gcggatattt cgtcgctgcg agaacgtatt caacagcaaa
     5941 tacgtggaga atatcactta ggtgatgttg atttgtatta tcccggtgcc agtcttggca
     6001 tagtagaagt cgatcctgaa acaaccgatg cagacagtgc cctgcatgct gccgatattg
     6061 cgatgtatca ggagaaaaaa cacaaacaga aaacaccttt tgtcgcgcat ccagcgctac
     6121 attcctgagg cgtattcaca tccttttgat tggtgataac atgcgaatcg gtattatttt
     6181 tccggttgta atcttcatta cagcggtcgt atttttagca tggtttttta ttggcggcta
     6241 tgctgccccg ggagcataaa gatgaaaaaa acaacgatta ttatgatggg tgtggcgatt
     6301 attgtcgtac tcggcactga gctgggatgg tggtaacgtc acctctaaaa aatagcaaag
     6361 gctgcctgtg tgcagccttt gtgcaattta agcgttaact tttaatcttc ctgtagataa
     6421 atagcacgac aatcgcacca ataacggctg aagcatttcg tctgcagtct gaaccgcagg
     6481 caaccatttt tcagcaagtc tgcgctggga atcgctggct ttatcccatt tgctccacag
     6541 tgcctcacga tcgtaatcgt gcgcttccag cgtggggcgg gcgatttcaa aatacccctg
     6601 gcttgtttct atttgctcag attttaataa caactcaaca cgtgctgccg ctggtagaac
     6661 tgatatatga aattccaaag cctttgaacc cgtacgcttg cgacgtagtt ctggatgatt
     6721 ggctgcaagc ttttcaagat tgcggcgaat actcacctca tgtttaggaa agcatgacat
     6781 tcctacgcat tcttgcgctg ttacccaatg ggttaacatg atgttttctc catataccga
     6841 gaaggccaaa tctgggctgg ttccatatcc aaatatctag caatgattgc ttcgtatcgt
     6901 ggtatatggc agcgaaatac gttacgcata gtgtcaggct ttaagcctgc ctctc
//
LOCUS       DAJTYI010000174         6894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYI010000174 DAJTYI010000000
VERSION     DAJTYI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..648
                     /locus_tag="OO564_003656"
     CDS             <1..648
                     /locus_tag="OO564_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654609.1"
                     /translation="SYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGA
                     SILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKL
                     HVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALP
                     TLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(787..1416)
                     /locus_tag="OO564_003657"
     CDS             complement(787..1416)
                     /locus_tag="OO564_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6654610.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(1533..3179)
                     /gene="fumB"
                     /locus_tag="OO564_003658"
     CDS             complement(1533..3179)
                     /gene="fumB"
                     /locus_tag="OO564_003658"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6654611.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(3257..4597)
                     /gene="dcuB"
                     /locus_tag="OO564_003659"
     CDS             complement(3257..4597)
                     /gene="dcuB"
                     /locus_tag="OO564_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6654612.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(5168..5887)
                     /gene="dcuR"
                     /locus_tag="OO564_003660"
     CDS             complement(5168..5887)
                     /gene="dcuR"
                     /locus_tag="OO564_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6654613.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5884..>6894)
                     /gene="dcuS"
                     /locus_tag="OO564_003661"
     CDS             complement(5884..>6894)
                     /gene="dcuS"
                     /locus_tag="OO564_003661"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6654614.1"
                     /translation="ILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAA
                     QELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVR
                     SNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSY
                     KQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSESQLPD
                     SGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPD
                     KIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERS
                     NR"
BASE COUNT         1640 a         1710 c         1763 g         1781 t
ORIGIN      
        1 tcatatgtta tcggtgatgc ggatttgttc ccctattatc tgtcgtatgc gggagctgct
       61 aacctggtga cgttagtatt cttcccacgc ttagttaaat cattatcccg acgcatttta
      121 tgggccggag catctattct tccggtgtta agctgtggtg ttctcctgtt aatggcatta
      181 atgagctatc acaacgtcgt cctcattgtg attgcgggta ttttgctgaa tgtgggaacg
      241 gcgcttttct gggtattaca ggtcatcatg gtggcagata ccgttgatta cggtgaatat
      301 aaactgcacg tacgctgtga aagcatcgct tactccgtgc agactatggt ggtgaagggc
      361 ggttcagcct ttgcggcttt tttcattgcg gttgtgttag ggatgattgg ctatgtaccg
      421 aatgttgaac agtctacgca agccctatta ggtatgcagt ttattatgat tgctctaccg
      481 actctgtttt tcatggtaac gctgattctc tacttccgtt tctatcgcct caatggcgac
      541 acgctgcgca ggatccagat tcatctgctg gataaatatc gcaaagtacc gcccgagcct
      601 gttcatgctg atattccggt cggtgcagtg agtgatgtga aagcctgacg tggacaatag
      661 ctaacgaaaa tggcctgatg cgatatgttt atcaggccac gtcttaatgt aatgctttgg
      721 atttgcaaat tttgtagacc ggataaggaa ttcacgccgc atccggcatc aacaaagtgc
      781 acgttgttat ccggttatca agccatagcg ccgtagctgg cggcaatgaa agcgagcgag
      841 caatatcact gtcgttaatg cgccaagcag cgcgcagaac atatcggatt gcgtgtccca
      901 ctggtcgccc tgggtaccga gaaaatcatc cgctccctgg cccatcgcca gtgccgccca
      961 ccactcgatt aattcataca tcgcgcttat cgccagcgcc acgcaacaga ccagaaacgc
     1021 caccatttta cgtccgcgca cgtacatccc gcgaacgaga atttctcgtg ccaccagtgc
     1081 aggcaccagc ccctggaaaa agtgccccag cttgtcatac ggattacgac tcaaccccag
     1141 ccattcctgc acctcaaaac caacggggac tttcgcgtag gtgtactgcc cgcctaccat
     1201 caggatgatg gcgtgaaaga agatgagcgt atagagcagc agcgttaacg ggtaacgtct
     1261 ggcggtggca agtagtagcg gcacgacgat aatcaccggt gtcacttcca gcagccaggt
     1321 gagtttgtca tgggccgaaa tgccggtata aatcaggatt aacgttagcg ccagtgcgcc
     1381 ggtgttaaga attaacggct tgagtgtgcg ggtcatggtg agttcgcaag tcagggaaaa
     1441 tactgactat tcaccagcaa gcatgaaatt acaatcgcaa aagaggtggc ccagcacctg
     1501 gcagcatgct gccaggtgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
     1561 ttgacgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa
     1621 tcttctactt caattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
     1681 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag
     1741 aagccgccgt gtttatgaca cgcgtcggta acctgctggc tgcggttgcc tttcgccagc
     1801 atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat acgacctgcg
     1861 gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag
     1921 tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc
     1981 agcttggcgt gtgcaatatc tcgacccaca ataatggtgc cagtgagcga caaacgagtg
     2041 gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcgacc
     2101 ttcaccgctt cgccttcacc ggcctgacgc agttcttgtg gaatgtactg acctagattg
     2161 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca
     2221 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
     2281 acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
     2341 gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc
     2401 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta atttgacggt tttcaggttg
     2461 gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta
     2521 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct
     2581 ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg
     2641 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg
     2701 gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag gttatcttcg
     2761 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
     2821 cgctggcctt ttttaccgac gatgaccgcg gtgccggtat cctggcaggt cggcagcacg
     2881 cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt gtcgttttcg
     2941 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg acggagcatg
     3001 aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
     3061 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat
     3121 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
     3181 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc
     3241 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
     3301 acgatacgct cacaccaatc aaccccggca gaataaaact gtggttgatg acgaagcgac
     3361 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
     3421 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc
     3481 cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
     3541 tggaaaccag cagcagaaca atggcatacg cccacggata ctctttcacc atttcaccca
     3601 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac
     3661 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
     3721 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
     3781 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
     3841 caagaagggc gactacagcg attgccccga ggaaaatcca cattgccagc cagttgcttt
     3901 tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
     3961 gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc
     4021 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcagat
     4081 cgaggaactc aagatggcga ccatcaaagg ttacattacc cagcatcgca accagagaca
     4141 cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
     4201 ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa
     4261 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg
     4321 cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga
     4381 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
     4441 gcatgacatc aactggtggt ttacctagct gaaggtggaa gacgaagacc agaatgacca
     4501 gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac
     4561 atattagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
     4621 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
     4681 gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt
     4741 ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa
     4801 actctctggc gatgggttta tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt
     4861 atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata
     4921 gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt
     4981 taattaactt ttgttatcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat
     5041 ttttaacgat gatggaggga taattatatt tgatctggca caagttttac tgattaacga
     5101 tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac agataaccag
     5161 cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac
     5221 ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga
     5281 ccagccagat gaggtatttt cggcaggaaa cacgcgagat gttaacctcg ttagccagct
     5341 cgtcggttga aaagtcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
     5401 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt
     5461 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
     5521 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
     5581 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
     5641 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
     5701 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgcatcg ctattgaaga
     5761 taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt
     5821 gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
     5881 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
     5941 ccccggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
     6001 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
     6061 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
     6121 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc
     6181 gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct
     6241 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
     6301 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
     6361 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
     6421 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
     6481 acgaagtgcg tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgaacttc
     6541 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
     6601 ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc
     6661 gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag
     6721 tttctcatcg tcctgcgact tacggtaatt caacaattct tgtgcggcat cgttgatcag
     6781 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc
     6841 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggat
//
LOCUS       DAJTYI010000175         6808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYI010000175 DAJTYI010000000
VERSION     DAJTYI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            357..1052
                     /gene="minC"
                     /locus_tag="OO564_003662"
     CDS             357..1052
                     /gene="minC"
                     /locus_tag="OO564_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6654615.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1076..1888
                     /gene="minD"
                     /locus_tag="OO564_003663"
     CDS             1076..1888
                     /gene="minD"
                     /locus_tag="OO564_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6654616.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1892..2158
                     /gene="minE"
                     /locus_tag="OO564_003664"
     CDS             1892..2158
                     /gene="minE"
                     /locus_tag="OO564_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6654617.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2530..2874)
                     /locus_tag="OO564_003665"
                     /pseudo
     CDS             complement(2530..2874)
                     /locus_tag="OO564_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2909..3028)
                     /locus_tag="OO564_003666"
     CDS             complement(2909..3028)
                     /locus_tag="OO564_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6654618.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2989..3174)
                     /locus_tag="OO564_003667"
     CDS             complement(2989..3174)
                     /locus_tag="OO564_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654619.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3275..3448
                     /locus_tag="OO564_003668"
     CDS             3275..3448
                     /locus_tag="OO564_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654620.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3450..3794
                     /locus_tag="OO564_003669"
     CDS             3450..3794
                     /locus_tag="OO564_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6654621.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3798..4133
                     /locus_tag="OO564_003670"
     CDS             3798..4133
                     /locus_tag="OO564_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6654622.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4190..6511)
                     /locus_tag="OO564_003671"
     CDS             complement(4190..6511)
                     /locus_tag="OO564_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6654623.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGSETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6628..>6808)
                     /locus_tag="OO564_003672"
     CDS             complement(6628..>6808)
                     /locus_tag="OO564_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654624.1"
                     /translation="LGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVKDIDNALVITT
                     GNGSYGYPLQERVVS"
BASE COUNT         1831 a         1610 c         1539 g         1828 t
ORIGIN      
        1 ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct ggcgtggtga
       61 aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct gtcacgatta
      121 gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca atgggttgat
      181 tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata gacttttact
      241 ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat gccttatagt
      301 cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag gccaggatgt
      361 caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt catctgcatg
      421 aggcagaacc taaggttatc catcaggcgc tgaaagacaa aatcgctcag gcccccacat
      481 ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg gtaaactggt
      541 cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta agtggctgca
      601 aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg acggaaggaa
      661 aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat acaatgccgg
      721 tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt tatgctccac
      781 aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt gccgatggga
      841 acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg
      901 aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca ggtgaatact
      961 ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggggcgactg cagttagtcg
     1021 aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt taacaaggaa tttctatggc
     1081 acgcattatt gttgttactt cgggtaaagg gggtgttggt aagacaacct ccagcgcggc
     1141 catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg
     1201 cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg atttcgtcaa
     1261 cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc gtactgaaaa
     1321 tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc gtgaaggggt
     1381 cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt gtgactcccc
     1441 ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag ccattattac
     1501 caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca ttctggcgtc
     1561 aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc tgttaacgcg
     1621 ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg tgctggagat
     1681 cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa
     1741 ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct acgcagatac
     1801 cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag agaagaaagg
     1861 cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct ttctctcgcg
     1921 gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg ctgaacgccg
     1981 tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc ttgaggtcat
     2041 ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc aaaaagatgg
     2101 cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc tgaaataagc
     2161 ccgctgtaaa aacgcattta tcttcaaggc agagtcattt ctgccttgag tttttcatcc
     2221 ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc attgctattt
     2281 ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg tttatgcctg
     2341 gttgagtaaa taaccatgtt gttagtgtgg atatgctggg agatgataac cccgcaattt
     2401 cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg gctttttttg
     2461 ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc gcagtttaat
     2521 cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg actccacacc
     2581 ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga atgaggcttt
     2641 aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat taccgtcact
     2701 attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct tcaggcaggg
     2761 ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac ccagttcacg
     2821 atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat gatagtagcg
     2881 tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca ggggattcat
     2941 cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc atgatgaaat
     3001 gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa gtgtagttgg
     3061 ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac aatgaggccg
     3121 tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa
     3181 atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc
     3241 aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat ttaagattat
     3301 tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct
     3361 gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgtg
     3421 tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta
     3481 tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc acgaaaggtg
     3541 cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag
     3601 gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg
     3661 cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca
     3721 atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg
     3781 gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc ctgtttgttt
     3841 tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg tcagaaacct
     3901 atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa
     3961 ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc
     4021 cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa
     4081 tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa taatatttca
     4141 tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat cagaacttat
     4201 atttcaggcc caccatcact gcggtgtcat tgtagccatt atcgcccagc tgcacgccca
     4261 cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt ttaatttccc
     4321 ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt
     4381 tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc
     4441 tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg atgttgttat
     4501 ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga tcgacgccca
     4561 tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct tccagaccaa
     4621 aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca cctttcgcgc
     4681 tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag ctatattgcg
     4741 cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa gtggcataca
     4801 gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag
     4861 ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactatcgg
     4921 taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc gaggtcgttc
     4981 tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaagtc
     5041 ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc gcacggtcgt
     5101 tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt
     5161 cggggcgcag gacggggata ggcgcaacag taggcgctga acttcctccg gagtttgttt
     5221 cactgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc gcttcggtat
     5281 tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt
     5341 ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg taagctccgc
     5401 tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca ttaataacga
     5461 ctttatccgt agctgaatta tcatcatcca attcagttct gagatacaat acgccattat
     5521 taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt ccatctgccc
     5581 gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag tcagctttaa
     5641 tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca tctgttgaaa
     5701 tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga cctttaatat
     5761 tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc acgttactcg
     5821 tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca gataaaatat
     5881 ctaccgttgc ttcctgacta tttatggaag actccagcac aattccagac ccctgatgcg
     5941 cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta gcaatcgcgg
     6001 cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc tggcgggcag
     6061 cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg
     6121 cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg tacgcggcat
     6181 aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt gctatggcat
     6241 tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat acacacgcac
     6301 gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa
     6361 taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag ttgactctta
     6421 ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt gagtaatccc
     6481 ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat tcacttacga
     6541 ttgcgctagc tgttaatgct gggcttagag gactattgtc agaaggatat aaaccgcccg
     6601 ttgttacaat gacgccattg ttaatggtta acttactacc ctctcctgca acggataccc
     6661 gtaacttcca ttacccgttg ttataactag cgcattatca atatctttta cttttccatc
     6721 ttttattgcc acagcatgtt tatattcatc gtgctgaata aactgttcag catatgcatt
     6781 acccaccgcc agagcaataa gtcccaaa
//
LOCUS       DAJTYI010000176         6386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYI010000176 DAJTYI010000000
VERSION     DAJTYI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1098..2045
                     /gene="icsP"
                     /locus_tag="OO564_003673"
     CDS             1098..2045
                     /gene="icsP"
                     /locus_tag="OO564_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6654625.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2607..2979)
                     /locus_tag="OO564_003674"
                     /pseudo
     CDS             complement(2607..2979)
                     /locus_tag="OO564_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3223..3342
                     /locus_tag="OO564_003675"
                     /pseudo
     CDS             3223..3342
                     /locus_tag="OO564_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3592..4458
                     /gene="ospB"
                     /locus_tag="OO564_003676"
     CDS             3592..4458
                     /gene="ospB"
                     /locus_tag="OO564_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6654626.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4787..5527
                     /gene="phoN2"
                     /locus_tag="OO564_003677"
     CDS             4787..5527
                     /gene="phoN2"
                     /locus_tag="OO564_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6654627.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(5880..>6386)
                     /gene="ospC4"
                     /locus_tag="OO564_003678"
     CDS             complement(5880..>6386)
                     /gene="ospC4"
                     /locus_tag="OO564_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6654628.1"
                     /translation="RIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEI
                     NNKVTDKKMAHQALAYSLGDKKADIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVE
                     YLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGA
                     VSGKRFER"
BASE COUNT         2015 a         1195 c         1227 g         1949 t
ORIGIN      
        1 atccaaactg ccaagcctct ttattataag taagatctgg cactggaata ttctcaaaag
       61 gactaacatc atcaccacga ccttcaacca accattttat tgcatttaaa tcccagaata
      121 gttcagtagt ggtcaatttg ggaaacgcac aatattgtac ggttacgtta tcgcaataga
      181 gtgaactccc gccctatacg gcgttttcaa ggattagtcc ttaatcggac aaccacatta
      241 aagcataata aataattaag ctttaataat atactataga tacagttcta ttataaaaaa
      301 tactatttta ggtgatgtat taaattagta gaatatttat tcttttataa aagcaccaat
      361 gaattgtcca agtaaacatg aggtaaaatg tgtagaggat aactgagttt ggtcacttta
      421 actgtattag tcgcagtctg actctcgact ttaaaaggat gggaattacc tgtactgata
      481 gtgagaaagc tttccattga aataagtcaa ataaaagttg atggcttcag tgctaaagtg
      541 aggtgcccgc tagccgtatt cactcgaatc ggaattcaat gtattgcttc tgccatttcc
      601 ataatatttc acgggaaaaa caaaatctga aaaactatac ttaagtgttg tattaagtca
      661 gttgagatac aaatacatat ttatcagcac acgttacgtc aaatacagga ggttgtcatg
      721 ttggtactga aaggcacgtt ggctattgca gccgtgatga caatttattg tcttatcgtt
      781 gttcttatgg aacgcttttc tgattgattt catattggcg agagaacaag aaggtgagta
      841 gaattgtcgc ggaatcctga aactatcagc ctcaggtatg aacaccaaaa ggtaggtaga
      901 gaaatctatg gcccccgtta ctttattcgc acaatgaggt atttttttat ttttacaaaa
      961 gttcaataaa tattataata aaagatatgt tcttggtaac ataaacaaat atttaattaa
     1021 aataatgctt ggcattgacg tttatcttat aaagtaagaa gatcatggac atttcaacca
     1081 aaaaagtaga gttctcgatg aaatcaaaat tccttgtact tgcactttgt gtacctgcga
     1141 tctttactac acatgctacc actaactatc cacttttcat accggacaac atcagtacgg
     1201 atattagtct aggatctctg agtggcaaaa caaaagaacg cgtttatcat cccaaggaag
     1261 gggggagaaa aattagtcaa ctggactgga aatacagtaa tgcgactatt gttagaggtg
     1321 gcatcgattg gaagctaatt ccaaaagtgt ctttcggagt ttccggttgg actactttag
     1381 gtaaccagaa agcaagcatg gttgataaag actggaacaa ttccaatacc cctcaggtat
     1441 ggaccgacca gagctggcat cccaatacgc atctccgtga tgctaacgaa ttcgagctga
     1501 atcttaaagg ttggttatta aataatttgg attatcgact tggactaata gcaggttacc
     1561 aagagagtcg ttacagtttt aatgcaatgg gagggagtta tatttatagt gaaaacgggg
     1621 ggaacaggaa taaaaaaggg gcacatccta gtggtgaaag aacaataggt tacaaacagc
     1681 tcttcaaaat accttatatt ggattaactg ctaattaccg ccatgagaat tttgagtttg
     1741 gagcagaact gaaatatagt ggttgggttc tttcatctga tacagataaa cactatcaga
     1801 ctgagacaat ttttaaagat gaaataaaaa accaaaatta ctgctctgtt gctgcgaata
     1861 ttggatacta tgtcaccccc agtgcaaaat tttatataga aggctccaga aattacattt
     1921 ctaataaaaa aggtgataca tctctttatg agcaaagtac caatatatct ggcaccatta
     1981 aaaatagtgc aagtattgaa tatattggtt ttctcacttc cgcaggtata aagtatattt
     2041 tttgaattgg ttcaccgagt tgattacgtt ttcatggcta aaaattgccg cccattgtga
     2101 gtcatattcg ccctgacttt ccagaaccat acgaactgcc cgttgacgga cttcggggga
     2161 aaaacgtgta tttttagtca ttctgtttac ctctttctca gggcgtttag tctccaggat
     2221 ttccggggcg gttcaaaaaa ctcttcgttg attactgtgg acttactgtt ggcattaccg
     2281 accctgagac cggagaaata agaaccgctt agatcatcgt ggctgttctc ggggtatcaa
     2341 gttacacatg ggcagaggct acccaaggtc tactattatc gcctgaaagc gctgatggaa
     2401 cagtgctatc agggacatgc tgatgaaaat acctaaataa tcagccagca ctctatcttt
     2461 ccaaatccac agcatagcaa agagagcaaa agagcctgta aattcagaaa ttaaggaaac
     2521 gcctcttggt gcacataatc tatcttatgt taaatgaaat ctaatttctt gattatatgc
     2581 tggatcgttc gtgggtggct ttgcccctat atttccagac acctgttatc attggcccgc
     2641 tgccgcagat attcccgtgg cgagcgataa tccagcgcac tatgcggatg ccattcgtta
     2701 taatgctcga atgcctctgc aaggttcttt gctgccgtta acccgtcagg tttgggcatg
     2761 atactgatgt agtcacactt tattgttttc acgaagctct ttgctattct gttactctcc
     2821 ggactccgca ccgccgtgtt cttcggttca acaaagtccc aacatccggg tgaactggcg
     2881 tgtttcatta gcccggtagc atgaaccatt atccgtcagt cactccactg gagacgacgg
     2941 aagatcgttg ccgaagcggt gttccaccgc tcccagcatg tcgccaatgg gagagcatct
     3001 acggtctgat tgaactgttt attcaaattg tccaccgtct gtcagtacgc tctgaacgtc
     3061 ggctggttaa aacactgctc gctgattttc agagggttca cggtaaaaca gcacttttat
     3121 tccggattgc ggaagcggct ctgaataatc cggatgggct ggttaaagag gttgtgtacc
     3181 atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac ttggcgcagt
     3241 tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta ccagaacaag
     3301 atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc tccacggtac
     3361 tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt tgaaattctt
     3421 tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa tataattgca
     3481 tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta tatgaaaata
     3541 taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg tatgaattta
     3601 gatggtgtta gaccatactg tagaatagtc aataaaaaga atgaaagcat atcagatatt
     3661 gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc aaaagcagca
     3721 ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc tattatggga
     3781 caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt taaaaatgaa
     3841 aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag tgaaccaata
     3901 gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt aaaaaaagcg
     3961 aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta tattcttggg
     4021 cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga tgtacaaaca
     4081 atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg ttttacttca
     4141 tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc tgaaagtctt
     4201 tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct agagcagata
     4261 aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc cggctatcat
     4321 ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg ttcaacttca
     4381 attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac ttttattatt
     4441 aataaagaac tggattagta taaaattttt aacctatagt taattgtttg catttaagat
     4501 gaagttattt tatctttagt agaaaagtgc ttccctatga gggaaaatca caaatcatca
     4561 taatacagag acaaaacgat acgaacaaat agataaaaaa catcattctt ttaccacatt
     4621 atttttcgtg aatatgaaaa ataatgttat tactttaata taagactatt ttttgttttt
     4681 ccatcactct gttcaaattt ttccgcatga cttgtgtttt ttgtaacaca gctcgttttt
     4741 tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga aaaccaaaaa
     4801 ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa atgctctgaa
     4861 ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac ttccgccgcc
     4921 tccggcagag gattcagtag tatttcaggc tgacaaagct cattatgaat tcggccgctc
     4981 gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg agaattttgg
     5041 tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc caatcttata
     5101 tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta acgccaaaga
     5161 atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta cacctgataa
     5221 agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct ttggttgggc
     5281 agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac ttcgacgtgg
     5341 atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg atgtagaggc
     5401 tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg aatttaccaa
     5461 aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca atgaactgac
     5521 cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa tctaccctat
     5581 atgagcatag gaggagtcta tgggcacacc acgttttact cctgaattta agggattact
     5641 ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg ctgccatcgg
     5701 aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctaggaaa ctcggggcgg
     5761 ttccgttcac cacatgcaat gtggtgttgc aggggaacgg tctgcaccat cccctatatc
     5821 gatcaatata acaaaaatga caacttcaga ttcagggcac aacctaaata tattttaggt
     5881 cacctctcaa atcgtttgcc tgataccgct ccatttttta ataaaaaatc aatcattttt
     5941 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc tttttgaaat
     6001 tttttgttta catcaaccaa tccattattg ataaaatatt ctagcacttt atagttggca
     6061 ccatctttac taagtaagta ttcaacatca tacagattac aatatatatt gttgttcatt
     6121 ttctccatct ctgcgatatc ttgttttgta aaattaaatt tagatagtaa ataaagtgct
     6181 atatcagctt ttttatcgcc taaagaataa gcaagggcct gatgagccat ttttttatca
     6241 gtcactttat tgttaatctc ttcataatta gatgctttaa tcgcatcctc caaagatatg
     6301 tctctgagtt ttattttttt gaagttatct gtacttagca ttcttggtat atgatactca
     6361 agctgaaaca caaagtttat aattct
//
LOCUS       DAJTYI010000177         6382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYI010000177 DAJTYI010000000
VERSION     DAJTYI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..423)
                     /locus_tag="OO564_003679"
     CDS             complement(<1..423)
                     /locus_tag="OO564_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR6654629.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGA"
     gene            741..1382
                     /gene="udk"
                     /locus_tag="OO564_003680"
     CDS             741..1382
                     /gene="udk"
                     /locus_tag="OO564_003680"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6654630.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1474..2055
                     /gene="dcd"
                     /locus_tag="OO564_003681"
     CDS             1474..2055
                     /gene="dcd"
                     /locus_tag="OO564_003681"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6654631.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2077..3930
                     /gene="asmA"
                     /locus_tag="OO564_003682"
     CDS             2077..3930
                     /gene="asmA"
                     /locus_tag="OO564_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6654632.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4382..5965)
                     /locus_tag="OO564_003683"
     CDS             complement(4382..5965)
                     /locus_tag="OO564_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6654633.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(6166..6315)
                     /locus_tag="OO564_003684"
     CDS             complement(6166..6315)
                     /locus_tag="OO564_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654634.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT         1611 a         1626 c         1666 g         1479 t
ORIGIN      
        1 ggcacccccc aacagaggcg gaacaatggc gctgccgaat gccagacgca gccagtcagc
       61 cagattttgc aaggggttat accacggcag caatttacgt agcagcactg ccccgaccac
      121 ggcttcaaca atattgatgg tcgtccaggt catgttcagc gagctggtgg aaaaaagcag
      181 gatggatgcg gcgatatttc ccagcgaaca gctcagcgca attcccggcc acatgcgccc
      241 ggcatggcga taaaacgcca ccatcatgat ggacgtcggg aaccacagtg gggcgagttg
      301 ggtgccaaac tgcgaaagct caagcgagac aagagtaaag ataaacgaga ctaaacctaa
      361 actgaccagg tgaagcagcg ggtggggcag ggcaattaat acatgctgtg attgtttgct
      421 tattaccgct ttatccgcaa ggccaggtcg ccagtattcg tttctgtgtt tatgctagta
      481 caaaagtttt acattttata tgcgaattgc tcataaagtg acgtaaaggc ggataatttg
      541 cgcaactgcg tataacattt tttaccttac ataaaactga tcaacgtaat ttgccaccgg
      601 gaattctttg tcaacaatta actctgagcg cgcggcaacg ctattcgact ggtatcagac
      661 ggatgaaatc cctataattg ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg
      721 ttaatcaggt cgctaaatat atgactgatc agtctcatca gtgcgtcatt atcggtatcg
      781 ctggcgcatc ggcttccggc aagagtctta ttgccagtac cctttatcgt gaattacgtg
      841 agcaagtcgg tgatgaacac atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa
      901 gccatctgtc gatggaagaa cgcgttaaga ccaactacga ccatcccagc gcgatggatc
      961 acagcctgtt gcttgagcat ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg
     1021 tttacagcta tgttgaacat acgcgtatga aagaaacggt gaaggttgag ccgaagaagg
     1081 tcatcattct cgaaggcatt ttgttgctga cggatgcgcg tttgcgtgac gaacttaact
     1141 tctccatttt cgttgatacc ccgctggata tctgcctgat gcgccgcatc aagcgtgacg
     1201 ttaacgagcg tgggcgttca atggattccg tgatggcgca atatcaaaaa accgtgcgcc
     1261 cgatgttcct gcaattcatt gagccttcta aacaatatgc ggacattatc gtgccgcgcg
     1321 gcgggaaaaa ccgcatcgcg atcgatatat tgaaagcgaa aataagtcag ttctttgaat
     1381 aagcttgata aattgtgtac cgttcagtga taagctggta tgcccttgac gtaattggcg
     1441 ttaagggagt gatgcgcgaa aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa
     1501 gcctggcttg atgaaggccg tttgtcgatc aacccacgtc cgccagtgga gcgtattaac
     1561 ggcgcgacgg tggatgtacg cctgggcaat aaatttcgta ccttccgtgg tcacacggca
     1621 gcgtttatcg atctgagcgg tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg
     1681 agcgatgaga tcgttctcga cgagggcgag gcgttctatc ttcacccagg agagctggcg
     1741 ctggcggtga cgctggagtc ggtgacgctg ccagccgatc tggtgggctg gctggacggg
     1801 cgttcctctt tggcgcgtct ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg
     1861 ggctggtctg gttgcattgt gctggagttc tacaactccg gtaagctgcc gctggcgctg
     1921 cgtccgggca tgttaattgg tgcgctgagc tttgagccgc tttccggccc ggcggcgcga
     1981 ccttacaacc gccgtgaaga tgcgaaatat cgcaaccagc agggcgcggt agccagccga
     2041 atcgataaag actaattcag gcccattgag gataccatga gacgatttct gacgacgctg
     2101 atgatactcc tggtcgtgct ggtggccggg ttatctgcgt tagtgttgct ggtgaatccg
     2161 aatgatttcc gcgactatat ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag
     2221 ctcgacgggc cactgcgttg gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg
     2281 tctctcaccg cccagggcgc aagccagcca ctggttcgcg ccgacaacat gcgtctggac
     2341 gtggcgcttt taccactact gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg
     2401 gcagtgatcc aactgacgcc gcagacggaa gcggtgcgca gtgaagacgc tccggttgca
     2461 ccgcgcgaca ataccttgcc ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc
     2521 agtcttaagg tggcggacag cgttctggtg ttccagcatg aagatgacga gcaggtgaca
     2581 atccgcaata tccgcctgca aatggaacaa gatccccaac atcgtggctc atttgagttc
     2641 tccgggcggg ttaatcgcga tcagcgcgat ctcacgatat cccttaacgg tacggtagat
     2701 gcttctgatt atccgcatga tttaacagcg gctattgaac aaattaactg gcagttgcag
     2761 ggtgccgatt taccaaaaca aggtattcag ggacagggga gtttccagtc ccagtggcag
     2821 gagtcacata aacgcctttc atttaaccaa attagtttga ccgccaatga cagtacgctg
     2881 agcgggcaag cacaggtcac gctgacagag aaaccggaat ggcagctgag actgcaattc
     2941 ccgcaactga atcttgacaa cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt
     3001 gccgcgcagc aggggcagag ccaatcaacg ttgccgcgcc cggtcatttc ttcgcgtatt
     3061 gatgaaccgg cctatcaggg actgcaaggc tttacagctg atattttgtt gcaggccagt
     3121 aacgtgcgct ggcgcggaat gaattttaca gatgttgcca cgcaaatgac caacaagtcg
     3181 ggtttgctgg aaattactca actgcagggc aagcttaacg gtggaaaggt ttcactgccg
     3241 ggcacgctgg acgcgacatc aataaatccg cggataaact tccagccacg gctggagaac
     3301 gttgagattg gtaccattct gaaggcgttt aactatccga tttcgttgac cggaaaaatg
     3361 tcactggctg gtgatttctc cggtgctgac atagatgccg acgcattccg ccacaactgg
     3421 caagggcagg cgcatgttga gatgaccgac acacgtatgg aagggatgaa cttccagcag
     3481 atgattcagc aagcggtaga gcgtaatggc ggtgatgtga aggccgctga aaacttcgat
     3541 aacgtgacgc ggcttgaccg ctttaccacc gatttgacgt tgaaggatgg cgtcgtgacg
     3601 ttaaacgaca tgcaaggtca atcgcctgtg ctggcgctga caggggaagg catgttgaat
     3661 ctggcagatc aaacctgcga cacccagttt gatattcggg tcgtgggtgg ctggaacggg
     3721 gaaagcaaac tgattgattt cctcaaagaa acgccagtac cgctgcgggt ttatggcaac
     3781 tggcagcaac tcaattacag cctgcaagtg gatcagttac tgcgcaaaca tctacaggac
     3841 gaagcgaaac gtcgcctgaa tgactgggcc gagcggaata aagattcccg caatggcaaa
     3901 gatgtgaaga agttgctgga gaagatgtag gttaccaggt gacctgtcct gtaggcctga
     3961 taagacgcgt aaagcgtcgc atcaggcgtt cgcggcggca ctattatatt ctgaatatgt
     4021 aagatcgaag gctgttccgt tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg
     4081 tgaacgtgcc agggtggctc aatcgccacc accctggcaa cccgggctcc cggcggtaaa
     4141 tcgccgctgg cgcggttccc tcatcttatt cctttcggct atcgggtacg ggcgcgaggt
     4201 aacatccctg taaaacgcgc cctcagccca catccatgtg ggctgcccca gcctacagga
     4261 aacgattcgg cgatttacag ccggacccac acccccgctg tgatgtccgt ttaccacaga
     4321 caacgcttca ttgctgaagt tataaggcgt tacgcagcat tgggtaaaat ggccgatacc
     4381 atcacacctc gtactccatc tcgccatcct tacgcagcgg tataatctgc accttctgca
     4441 cgcgatggct ttccacctgc aacgttttaa gcaaataatc ccccacctga acttcttcgc
     4501 caggttttgg aatacgctgc aaatactcca tcaacagccc ggcaatggtg tgatattcac
     4561 gtttttcatc cagcggcagc ggcacatatt gcaccagatc ctccagcggc atatgaccat
     4621 tcgccgtcca ggaaccgtcc gcattcttct gaatatcatg gcgggcgtcg atctcttcca
     4681 cttcgttcgg taagttaccg gcaatggttt cagtgacgtc acttaatgtc acaatccctt
     4741 ccaccgagcc aaactcatcc accacaaaag caaagtgcgt gcgggcatta cggaactgct
     4801 ccagggctgg taacaacggc aaggtttccg ggaacaccag cggctggcga atcaacaccc
     4861 gcaggttgag cggttcgccg cgcagtgact gttgtagcag gtcgataacg tgaacaacac
     4921 cgagcaaatc ttctgcgtca tcaccgtcgg taaccaccag ccgcgtatgc tgatttcgct
     4981 ccagcaactg gcggatttct tcttccggcg cgttgagatc gatatgctca atatcgtggc
     5041 gcgacgtcat aatgctgctg acggtacgct ggttaagatt aagtacccgc tcaatcatcc
     5101 gccgttcctg cggattaaag atctgctggt ttccatgatc caccagcatg gacgcggttt
     5161 cggcgtccag ttccgcatct tctttttgcc cgctaagcag gcgcattacc gcttcggtag
     5221 tccgctggcg tagcgtctga ttagcggaaa gaaaacgtcg ccggttaaag atagccaact
     5281 gattgagtgc ctcgatcatc accgagaaac caatggcagc gtacaggtag cctttcggaa
     5341 tgacgaagcc gaaaccttct gccaccaggc taaagccaat catcaacagg aagcttaagc
     5401 agagaataac gatggtcggg tgactgttaa caaattgcgt taacggcttg ctggccatca
     5461 acatcaggct gatagcgata accacggcgg ccatcatgac cagtaaatgg tcgaccatcc
     5521 cgacggcggt aatcactgag tcgagtgaga aaatggcgtc cagtaccaca atttgcgtca
     5581 ccacgcccca gaacttcgca cctttgcgtt gtgtgggatt attgctgtct ttcccttcca
     5641 gccgttcgtt cagctccatc gtggctttga acaacaggaa gaaaccacca aacagcatga
     5701 ttaagtcgcg ggcgctaaag gtaaacgagc ggaagctgaa cagcggttga gtcagggtga
     5761 ccagccagga gattgacgct agcagtaaca ggcgcattaa catcgccagc agcagcccgg
     5821 taacccgtgc gcggtcgcgc tgcttcggcg gtagtttttc ggcgaggatg gcaataaaga
     5881 ccaggttatc aatgccgagg accagttcga tcacaatcag cgtgattaac ccggcccaga
     5941 tagacggatc ggcaatccat tccatagtaa gtacaaaacc tattcgttgt atgacaaatg
     6001 tcacaattcg atcatggata aatcggggca aaaatgcaat gttgcagcac tatttatctc
     6061 atattatttg agggtaataa cctcacatta tccctgaatt aaaagcggta ataataaaac
     6121 attatttaag aaatatcgca tgtggagacg caatattaaa tattgtcaac ctaaagaaac
     6181 tcctaaaaac catattgaat gacacttaat ataattctta aaaatagcca attgccgaat
     6241 tgttaccttg cctgctattc cgttagctgt aacacttcct cctgcattat tggcgagcca
     6301 atattcaatt accataatgt caccttatat tcggaagtta atttccgcaa taaccctttc
     6361 tcatcaacga ttgtatgcag ag
//
LOCUS       DAJTYI010000178         6368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYI010000178 DAJTYI010000000
VERSION     DAJTYI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..73)
                     /gene="csgB"
                     /locus_tag="OO564_003685"
     CDS             complement(<1..73)
                     /gene="csgB"
                     /locus_tag="OO564_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6654635.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGY"
     gene            827..1477
                     /gene="csgD"
                     /locus_tag="OO564_003686"
     CDS             827..1477
                     /gene="csgD"
                     /locus_tag="OO564_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6654636.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1482..1871
                     /gene="csgE"
                     /locus_tag="OO564_003687"
     CDS             1482..1871
                     /gene="csgE"
                     /locus_tag="OO564_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6654637.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1896..2312
                     /gene="csgF"
                     /locus_tag="OO564_003688"
     CDS             1896..2312
                     /gene="csgF"
                     /locus_tag="OO564_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6654638.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2339..3114
                     /locus_tag="OO564_003689"
                     /pseudo
     CDS             2339..3114
                     /locus_tag="OO564_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3178..3669)
                     /locus_tag="OO564_003690"
     CDS             complement(3178..3669)
                     /locus_tag="OO564_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6654639.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3771..4325)
                     /gene="ycdY"
                     /locus_tag="OO564_003691"
     CDS             complement(3771..4325)
                     /gene="ycdY"
                     /locus_tag="OO564_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6654640.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4349..5086)
                     /gene="ycdX"
                     /locus_tag="OO564_003692"
     CDS             complement(4349..5086)
                     /gene="ycdX"
                     /locus_tag="OO564_003692"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6654641.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5141..6079)
                     /gene="ghrA"
                     /locus_tag="OO564_003693"
                     /pseudo
     CDS             complement(5141..6079)
                     /gene="ghrA"
                     /locus_tag="OO564_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
BASE COUNT         1764 a         1523 c         1408 g         1673 t
ORIGIN      
        1 cataacctgc tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca
       61 atttgttttt catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc
      121 aacttggttg ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc
      181 tgcggaaata tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca
      241 aagtattaaa acccgcgtaa atacgctgtt atctacgcaa aaaatatttt gttttctctt
      301 aaatctccgt tttccgctat caaaaatcac cagacagtca ttcttcttgc ccgtcgctga
      361 ttgctgcgat atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaaa
      421 ttgtccacgg aggtgtggag aaaaaacaaa aacgttttac atgacgaaag gactacaccg
      481 aaatattttt tatatgtatt attagtaagt tagcaccatt tgtatgattt tttaaaattg
      541 tgcaataaaa accaaatgta caacttttct atcatttcta aacttaataa aaccttaatg
      601 ttaacatttt aatataatga gttgcatttt gttacatgtt taacacttga tttaagattt
      661 gtaatggcta gattgaaatc agatgtaatc cattagtttt atattttacc catttagggc
      721 tgatttatta ctacacacag cagtgcaaca tctgtcagta cttctggtgc ttctatttta
      781 gaggcagctg tcaggtgtgc gatcaataaa aaagcggggt ttcatcatgt ttaatgaagt
      841 ccatagtatt catggtcata cattattgtt gatcactaaa ccttctttgc aggcgacagc
      901 tctcttgcag caccttaaac aatcgctagc aattacagga aaattacata atattcaacg
      961 ttctctggac gatatctctt caggctctat tattcttctg gatatgatgg aagcggataa
     1021 aaagcttatc cattattggc aggatacttt gagcaggaaa aacaacaata tcaaaatatt
     1081 gttgctaaat acgcctgaag attacccgta ccgcgacatt gaaaactggc ctcatatcaa
     1141 cggcgttttt tatgccatgg aggatcaaga acgtgttgtc aatgggttgc aaggcgtcct
     1201 gcgtggcgaa tgctacttta cgcaaaagct tgccagttac ctgattacac attctggtaa
     1261 ctatcgttat aacagcacgg aatcagccct ccttactcat cgggaaaaag agatcctgaa
     1321 taagctgcgt atcggcgcgt ctaataacga gatcgctcgt tcgttgttca tcagcgaaaa
     1381 tacggtaaaa acgcatcttt ataatctttt caagaagata gccgtaaaaa accggacaca
     1441 agcggtttcc tgggcaaacg ataacctcag gcgataaagc catgaaacgt tatttacgct
     1501 ggattgtggc ggcagaattt ctgttcgccg cagggaatct tcacgccgtt gaggtagaag
     1561 tcccgggatt gttaactgac catactgttt catctattgg ccatgatttt taccgagcct
     1621 ttagtgataa atgggaaagt gactatacgg gtaacttaac gattaatgaa aggcccagtg
     1681 cacgatgggg aagctggatc acaataacgg tcaatcagga tgttattttc cagacttttt
     1741 tattcccgtt gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt caaactgaag
     1801 aagcactaaa tcgtcgccag ataaatcagg cgttattaag tacgggcgat ttggcgcatg
     1861 atgaattcta aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat gcagtagttc
     1921 tactcatgct tatttcgcca ttaagttggg ctggaaccat gactttccag ttccgtaatc
     1981 caaactttgg tggtaaccca aataatggcg cttttttatt aaatagcgct caggcccaaa
     2041 actcttataa agatccgagc tataacgatg actttggtat tgaaacaccc tcagcgttag
     2101 ataactttac tcaggccatc cagtcacaaa ttttaggtgg gctactgtcg aatattaata
     2161 ccggtaaacc gggccgcatg gtgaccaacg attatattgt tgatattgct aaccgcgatg
     2221 gtcaattgca gttgaacgtg acagatcgta aaaccggaca aacctcgacc atccaggttt
     2281 cggttttaca aaataactca accgattttt aagccccagc ttcataagga aaataatcat
     2341 gcagcgctta tttcttttgg ttgccgtcat gttactgagc ggatgcttaa ccgccccgcc
     2401 taaagaagcc gccagaccga cattaatgcc tcgtgctcag agctacaaag atttgaccca
     2461 tctgccagcg ccgacgggta agatctttgt ttcggtatac aacattcagg acgaaaccgg
     2521 gcaatttaaa ccctacccgg caagtaactt ctccactgct gttccgcaaa gcgccagggc
     2581 ttacaaaacc tgcttaacga gcgcaaggtt attcgtgcgg cacaagaaaa cggcacggtt
     2641 gccattaata accgaatccc gctgcaatct ttaacggcgg caaatattat ggttgaaggt
     2701 tcgattattg gttatgaaag caacgttaaa tcgggcggtg ttggggcaag atattttggg
     2761 atcggtgcag acacgcaata ccagctcgat cagattgccg tgaacctgcg tgtcgtcaat
     2821 gtgagtaccg gtgagatcct ctcctcggtg aacaccagta agacgatact ttcctatgaa
     2881 gttcaggccg gggttttccg ctttattgac taccagcgct tgctggaagg ggaagtgggt
     2941 tacacctcaa acgaacccgt tatgctgtgt ctgatgtcgg ctatcgaaac aggggtcatt
     3001 ttcctgatta atgatggtat cgaccgtggt ctgtgggatt tgcaaaataa agcagaacgg
     3061 cagaatgaca ttctggtgaa ataccgccat atgtcggttc caccggaatc ctgacagata
     3121 aaaatgccgg aattaataat ccggcattta ctgtttaagc gaaagcgtga gtgcccctca
     3181 cgctttattt ttgatttcct gctcacggtg agccgtcttt gcactacgcg ccgccagcca
     3241 caggccgctg tttttcattg cgtaaccaaa taccagccca agcgccagcg aaggtaacac
     3301 cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg cctataaacg tccccggaac
     3361 aaatgaaagc agcagctgtt tggcctgaat acacatcaga aacgcgacaa taccggttat
     3421 gacataaccg agaatttcca gatgtggtgc cagtgcacta ccgtaaataa tgaccatcgc
     3481 ccacaccacg ccactaagca gggttgcagc ggagatcgcc agccctttca gcccaccttg
     3541 cgggcaagca aagtaggccg tacagcccag gaagcccgcc cagcttagta aacccaggga
     3601 aacagccacc catccccaga taccggagag aatgcctgtt gtgattgcaa tagagagaag
     3661 tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg aatgagatcg agcacacatt
     3721 ttatgcaaca acaaattatc tgttgcagtt atgagtgacg atgatcacat ttactcttca
     3781 gaatcttctt ccagctcgtc ccacattgca ctaatggcat cacgggttag cggtgccatg
     3841 gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc cgaggaacgc accacaccag
     3901 ggtaacagat actcactgaa cagtgtttcc agtgcttcgc tctcatcttc cgttgactga
     3961 tcttccagcc aggaagccgc aagcagcaat gtgccgatgt gatcggctgg cgtatccgcc
     4021 agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg cttccgttgc gtcctcaacc
     4081 catgcgctac gatatggcgg cacggcacat tcatcgccga taaacaacgc attgtaatcg
     4141 gcagagactt gcgtcatatc acagcttttc tgtaaacgcg tcagtaactc atcctgctcc
     4201 agtggccagt tcgcagccag tttcccctca cgaatcagag taaacagcgg caccagtaaa
     4261 ggatcttgcg gttggcggta atagagcgaa cccagcacac gacagaggat agaaaactcg
     4321 ttcattaata tatttccatt actagtaatt aaaggtctgc aaattccgca atcggtgcca
     4381 taccgcgaga ttcaaggaag ttcagtaagc ggcgcggaga aacattcaaa atgcgctctg
     4441 gcggaaaatc taccgcgtcg aggattttaa gacactcttc aaattccccc atggtaaacg
     4501 cggtgtgaga atccgagcct aatgccaccc aacctccagc atcacgtacc gctgcagcta
     4561 ccgcacgaca gttgtcttca ctgcccttac gtgagtgtaa aaatgaggaa ttattgattt
     4621 ccagcgccac ctgatgtttc gcggctgctt cagcaacggc tttaacatct atttcatatt
     4681 tcggatttcc gggatggctt attatatgca cattgccgct ggcgatagtg gcgatcatcg
     4741 cttgtgtatt ggtcgctttg tcatgtggcg caaaaaccgg ctcatgaaaa ccggcaataa
     4801 tcagatccag cgagtcaaac attttaccgc tgcagtcaat ttcaccatca acatttttaa
     4861 tgttagcttc aatgccgcgc aggatcccta ccccatcaac cactcgcggc cagatacgca
     4921 tgttaatgaa gtgccagtga tgcggcgcat cttccatatc cgggccatga tcggtgatcg
     4981 caaaaagttt aatgcccttt tgtttggcct gggcaatgta atcactcaat gtgctatatg
     5041 catgtgtgct ggcaacggta tgcatatgaa ggtcgacggg atacataact ctctcctgta
     5101 ttcaattccc gccaaggata gcaggaatcc tgatgcttta ttagtagccg cgtgcgcggt
     5161 cgacttgccc gcagaccctc tccccttttt cgagctgggc aatagtgcga gaaatgtact
     5221 ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg tgttatcgtc acgcgtggat
     5281 gttgccagag cggactttca ggcggtaagg gttcacgatt aaaaacatcc agcattgcgc
     5341 ctttaacttt cccgctatcc agcgccgcga gcaggtcatc ttccacaaca tgaacaccac
     5401 gcgccaggtt gaggagatac gcgccatcag gtaatttttc gagtaattgt tgattaataa
     5461 tgccgacggt ttcaggggta ttcggtaaca aattaatcaa tacccgacat tggctcagaa
     5521 atgcagacag ttcttcccgt ccggcaaagc tttgcacgcc aggccacgat ttacgggttc
     5581 gactccagca acgcagcgga aagcgctagg tttgcagact ctgagcaact ttacagccca
     5641 gtacgcctgc gcccaaaatg ccgatggtaa aatcttcccg atgatattca ggcagcggtt
     5701 gccaatgcga actattttgc tggatgcgat aatcatcaaa acgtcgaaac caatgcagca
     5761 cctgactgac agcatattcc tgcatttgct cgcccatacc ggtatcttcc aggcgaaaaa
     5821 gtggaacaga agggttcagc atttcagggt gtgcctgtag cttgctcaaa atagaatcaa
     5881 caccggcccc gagtgcgaac accgctttaa gatcgcgccc tgccagcatt tcaacaggag
     5941 gatgccagac taaaacataa tcagcagagt cattatctcc gcttttccat gccctgactc
     6001 ttgcctgagg aatagctttg cgcagtgcct caatccacca ttgggtatcg aacgttgggt
     6061 gataaaagat gatatccata ctgactcccg aaaagcgttg tgcgaaattt attcgcactt
     6121 atcgttatga tctacaaagg ccaccagcat aacaaatccg tggtcggtgg caaaaaaagc
     6181 agatttcgct tattaaaacc acacattgat tgaagtttga ataaacgcgc gattctttca
     6241 aaaagtttgt tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag
     6301 gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata
     6361 cggcaacg
//
LOCUS       DAJTYI010000179         6363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYI010000179 DAJTYI010000000
VERSION     DAJTYI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..419)
                     /gene="phoU"
                     /locus_tag="OO564_003694"
     CDS             complement(<1..419)
                     /gene="phoU"
                     /locus_tag="OO564_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6654642.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQML"
     gene            complement(434..1207)
                     /gene="pstB"
                     /locus_tag="OO564_003695"
     CDS             complement(434..1207)
                     /gene="pstB"
                     /locus_tag="OO564_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6654643.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1299..2189)
                     /gene="pstA"
                     /locus_tag="OO564_003696"
     CDS             complement(1299..2189)
                     /gene="pstA"
                     /locus_tag="OO564_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6654644.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2189..3148)
                     /gene="pstC"
                     /locus_tag="OO564_003697"
     CDS             complement(2189..3148)
                     /gene="pstC"
                     /locus_tag="OO564_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6654645.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3235..4275)
                     /gene="pstS"
                     /locus_tag="OO564_003698"
     CDS             complement(3235..4275)
                     /gene="pstS"
                     /locus_tag="OO564_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6654646.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4523..5596)
                     /locus_tag="OO564_003699"
     CDS             complement(4523..5596)
                     /locus_tag="OO564_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6654647.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(5607..>6363)
                     /locus_tag="OO564_003700"
     CDS             complement(5607..>6363)
                     /locus_tag="OO564_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6654648.1"
                     /translation="SGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDG
                     SYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEIDN
                     MRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEF
                     VTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTL
                     SWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
BASE COUNT         1612 a         1655 c         1501 g         1595 t
ORIGIN      
        1 tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc
       61 tgctgggaga atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc
      121 tccagctcgg caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc
      181 tgacgtttgg cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg
      241 ttcttgtcgc cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg
      301 attgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata
      361 ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata
      421 acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg
      481 gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaatg
      541 ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt
      601 aatcctgctt cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg
      661 ccgaacacgg ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac
      721 acagacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg
      781 tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac
      841 gggagagctt ctcaaacaga cgaacgccaa aggcgatgtt gtcgtagatg gacatcggga
      901 acggcgtcgg tttctggaac accatgccca ctttcgcacg tagcagggcg atatcctgag
      961 agttggtcag gatgttgtcg ccatcaagca gaatttcacc ttccgcacgc agctccgggt
     1021 acagttcaaa cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc
     1081 cgataaacgc cgtaacctgg tttttggcga tatccaggtt gatgtttttc agggcatgga
     1141 atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca
     1201 tactcattgc aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta
     1261 gggccgggta aggcgttcac gccgcatccg gcaaagtgtt aaccgtgttt attcttcgca
     1321 aaaacaacgc gcgccagaat gttcagcagc aggacgcaca gggtaatgat caataccccg
     1381 gcccaggcca gttgttgcca ttccgcaaac gggctcatcg caaacttaaa gatcgtcacc
     1441 ggcaggttgg cgatcggctg catcatgtcc gtgctccaga actggttgga gagcgcggta
     1501 aacagcagcg gcgcggtttc accggcaata cgggcaatcg ccagcaggat accggtcata
     1561 atcccggaca ccgacgcttt cagcgtaatc gcggagatca tcttccactt cggtgtaccc
     1621 agcgcataag ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt ctcggtggtg
     1681 cggataacga tcggcacctg caacagcgcc agcgcaatta cgcccgccca gccggagaag
     1741 tgctccatct gtgccaccac gatggtgtag acaaacagac caaccacaat cgacggcgcg
     1801 gagagcagaa tgtcgttaat gaagcgaatc acttctgcca gccaggattt tcgaccatat
     1861 tccgccagat aaatccccgc cataatgccc agcggcgtac cgaataccgt ggcccacaaa
     1921 attaacagcc cgctacccgc cagggcgttc gccagaccac caccttccgt attgggcggc
     1981 ggcgtcattt cagtgaacag cgccagcgac ataccgtcga taccgcgagt gatggtggac
     2041 attaaaatcc agatcagcca gaacaggccg aaggccatcg tcgccatcga gagcgtcagc
     2101 gcaatacggt ttttgaggcg gcgacgcgcc tgcattttgc ggcgagattc agccagcgcc
     2161 gcagtggttt gcatttcaac catagccatt agcgtgcccc ctcattctta gccaggcgca
     2221 taatcataaa cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg atcaggccca
     2281 gttccatcag tgcggcaacg tgcagaccgg attccgcttc cgcaaattcg ttcgccagcg
     2341 cagaggtgat actattgccc ggcatgtaca gcgaggcgct gtcgagctgg taggtgttac
     2401 cgatgataaa ggtcaccgcc atggtttcac cgagcgcgcg gcccagcccc agcatgatgc
     2461 cgccgataac accatttttg gtgaacggaa gaacgatacg ccagataact tcccaggtgg
     2521 tgcagccaat accgtaggcc gactctttca tcatcaccgg ggtttgttcg aacacatcac
     2581 gcattaccgc cgcaatgtac ggaataatca tgatggcgag gatcacgcct gccgcgagga
     2641 taccgatacc aaatgcgggg ccagagaaca gcgcgccaac aatcgggata ttcgacatga
     2701 tattgccgac cggctcctga aagtaaacgg cgaacagcgg cgcaaagata aacaggcccc
     2761 acatgccgta aacgatactt ggaatggctg ccagcagctc aatggcgata cccagcgggc
     2821 gtttcagcca gccaggcgca agctcagtca ggaacagggc gataccgaaa ctcaccggga
     2881 cggcgatcag cagcgcgata aacgaagtca ccaacgtacc gtagatcggc accagcgccc
     2941 cgtagatatc gttcggtgca tcccactctt tggtccatag gaaagccaga ccaaatttct
     3001 gaatgctcgg ccaggaggag atgatcagag agacaataat gccacccaac atcaatagca
     3061 caatcagcgc cgccagtttt accagcacgc tgaaaattat gtcgcccttt ttacccggtg
     3121 ggttaaaagc gggcttggtt gcagccataa gttactcttc agttaaacgc gtttacaaag
     3181 tcattttgta atgccggatg cggcgtaaaa caccgtaccc ggcctggagt tttattagta
     3241 cagcggctta ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa cctgttcaac
     3301 tacactatcc ggcaggctag cgtaatccag gtcgttcgcc tgtttagccc cggttttgta
     3361 cgcccagtcg aagaatttca gcacctctgt gccttgttct ggtttcttct gatctttgtg
     3421 gatcagaatg aacgtggtag aggtaatagg ccatgcatct tcgcctttct ggttggtcag
     3481 atcctgagcg aaggttttgc tccagtctgc accttttgct gcattagcga agttttcttc
     3541 ggtcggacta accggtttac catcagcgga gatcagtttg gtgtacgcca ggttgttctg
     3601 cttcgcgtaa gcatattcaa cataaccaat tgcacccggc agacgctgaa cgaacgcggc
     3661 gataccgtcg ttacctttac cgcccagacc gatcggccat tttacggtag agccagtacc
     3721 aacgttgttt ttccactctt cgttcacttt ctccaggtag ctggtgaaga cgaaggaagt
     3781 cccggagcca tctgcgcggc gtactacagc aatgttttgt gaaggcagtt tcagacccgg
     3841 attcagtttg gcgatggctt catcatccca cttcttgatt ttgcccaggt agatgtcgcc
     3901 gagggtttta ccatccagca ccagttcgcc agacttcagc cctggaatgt taaccgccag
     3961 caccacgccg ccaatcacgg tcgggaactg gaacagacct tcctgagcca gtttttcgtc
     4021 agacagcggc gcgtcagagg caccaaaatc aacggtatta gcgataatct gttttacgcc
     4081 accggaagaa ccgataccct ggtagttaac tttattaccg gtttctttct ggtaagtgtc
     4141 agcccatttg gcatacaccg gcgcagggaa ggttgcacct gcacctgtca ggcttgcttc
     4201 tgcaaacaca gagaaagcac tcatcgataa ggtcgcggcg acaacagttg cgacggtggt
     4261 acgcataact ttcataatgt ctcctgggag gattcataaa gcattgtttg ttggctacga
     4321 gaagcaaaat aggacaaaca ggtgacagtt atatataagg aatatgacat ttttatgaca
     4381 gaaaggaatt accaaataac ttactgattt taaatgtgaa gatgaaaatc ccacatgcca
     4441 tctttttgtc atgatgcatt taaaagtgat aaagaaatgg gtgagatgac aacgatgtga
     4501 tgaacaatcg ttgtcagacc tattagcgca caatctcaac agttatcgtc gctgttgcag
     4561 taaattgccc cggggctggg cgagctccag taaggctcgc aggagcagct gaaaaagtta
     4621 cactacctga tttttcacca tacactgttg atttatccat gtctgttggc aactcaccac
     4681 cgttcacttt gatttcctta ttatttttgt cgtaaatttt tatccccacg ttagagtttg
     4741 aagttacgac cattgaattg tccgtattgg gttcggtagc ataaaaagac gtactgatat
     4801 catcggtatt cagtatccct aggttttcac actcaatagt gactgtttta gtaattttat
     4861 gtgttgttac cgcacttcca acaacagatg aaaaatctgc agctggtatt tctttaaaat
     4921 caaaacaaac tggctttaaa ttatttattt cgcaactttg cggagctgta atatcgcccc
     4981 gaatataaac atcagccatc ggctgtgagt aatccaccgg ggtgtcacgg ctaattgttc
     5041 catacaatgt cactatcttt gtttcaggaa taatcaattt tcctgcaaat gtcttcttga
     5101 tataaaaata tactatcgct ttagccccac tggatatact taaaggttca atgcggttgc
     5161 aattgtactt tgtattacca gacgggttgt tagcatgata ttcaaaaggt gcattcacat
     5221 acccaacccc aagaatatct atagcaatcc caacatcaag gttcggatta agatcataat
     5281 aattaatatt agagtgaacc ccagcataag ttaatgcctg gtttattttt gacgtataat
     5341 agatataggg gaattctcct acgttaccag tatcacaatg acagcgtcca agatatgagg
     5401 atccattagt tacagtggtg ttaaatgtcg ttccaacctt attttcgttc gcggtaataa
     5461 gatatcccga gaaatcaagc gaactgtgaa atacgccatt atcaggggta cagatacctt
     5521 taggagctgc aagtgctaca ctacttaacg agagcataaa cacagaaaag caccactgtt
     5581 tgatatactt gttcataaaa atccttttaa tgacatgtca caccctgttc aattaatccg
     5641 ctctcacttt ctggtgtcga aagtgaataa ttgaagatgc attttgttga ggcgttttct
     5701 ccccaggata acgtaacctg tcctttttct ggtagaccag aaagataaag ttcgccacgg
     5761 tcaccgacaa ttcctgaatc tgccaaactt gcatcttgta tcgcagcaat ggctccaaaa
     5821 ggtaccggtt taccatttgc atttaacact ctgaagagaa cgcgataacc cacatgagtc
     5881 acaaactccg ctttaaccac tgcaccacgc gtgggaacca tcgtaacgac ggtattatca
     5941 agttctatat tcgacttcga gaaatagtta tcgctgattg ctacccgatt ttcatcataa
     6001 gggtttagct gtgtcttgac cgtatagccg cgccagtccg tcgcagcacc gcgcatatta
     6061 tctatttcta acccggcggc cccaggtgct ttaacaagag caatagtttc acctaactcc
     6121 tgacttaacg ttaccccttc gctgtgtgcc agaattcccc ctctgatacc ataattcagc
     6181 ctctgggaat tttggctgta gttatagctg ccgttcaccg tgccataaga gccatcatag
     6241 gtcagggaca tattaccgcc aacctcatca tcatgactgt tataggactg cattaaattc
     6301 catgaaaggt tatttctttc cagcgccgta ccgctgagtc cgagttgatg tgagctaccg
     6361 gaa
//
LOCUS       DAJTYI010000180         6323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYI010000180 DAJTYI010000000
VERSION     DAJTYI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            380..1558
                     /gene="ltrA"
                     /locus_tag="OO564_003701"
     CDS             380..1558
                     /gene="ltrA"
                     /locus_tag="OO564_003701"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6654649.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1641..1820
                     /locus_tag="OO564_003702"
                     /pseudo
     CDS             1641..1820
                     /locus_tag="OO564_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1850..2332)
                     /locus_tag="OO564_003703"
                     /pseudo
     CDS             complement(1850..2332)
                     /locus_tag="OO564_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2588..3568)
                     /locus_tag="OO564_003704"
     CDS             complement(2588..3568)
                     /locus_tag="OO564_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6654650.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3568..4767)
                     /locus_tag="OO564_003705"
     CDS             complement(3568..4767)
                     /locus_tag="OO564_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6654651.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5089..5427)
                     /locus_tag="OO564_003706"
                     /pseudo
     CDS             complement(5089..5427)
                     /locus_tag="OO564_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5494..6275)
                     /gene="istB"
                     /locus_tag="OO564_003707"
                     /pseudo
     CDS             complement(5494..6275)
                     /gene="istB"
                     /locus_tag="OO564_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1696 a         1462 c         1494 g         1671 t
ORIGIN      
        1 acgtggggtt cgggaagctg aacgaaagta aactgtccga tgaagcatcg aaatcttaga
       61 tgatgccaaa atggggccgt acagccgtcc cctgataagc gcagaagaca cctgcttact
      121 gactgcgcgg catccggtgt gaaggcagca ggatcccggg ccagaccatc tcagggaacg
      181 tgagaacctg tttccggcac gtatcgggaa acaggggcgg acccattcgt agtagtgatg
      241 aaagatctgt aatgggtctg gagcgaaggg atggggatca cattcattgc agaagacaca
      301 acccaaaagg ggatgacgta actgatgcaa cgaaaatcat ttgaaatacc aaaagcactg
      361 gtctgggcat catatctgga tgtgcgtcgc aacaagggag ccccccggat gcgacgggca
      421 gactttgaaa atgttcgacc agcaacgtga tggcaatcta tacaagatct ggaaccggct
      481 atgttcggga acatggtttc cgccaccagt gctggaaaaa cggatcccga agtcgaatgg
      541 caaggaacgc attctgggga tcccaacagt atccgatcga attgctcagg gagcgataaa
      601 acttttcatg gaagaaaagc ttgacccgat tttccatgct gattcatatg gttaccgacc
      661 aggcaaatca gctcatgacg ctctgaaaca atgtgccatc cggtgctggc gttatagctg
      721 gatactggaa gtggacatca gtgcgttttt cgaccatgtg agacatgatc tggtgctcaa
      781 agctctggaa caccacggga tgcctaaatg ggtcatcctg tactgtcgac gctggatgga
      841 agcgccaatg cagagttgtg aaaatggaga attaataacc cgaacacgag gcactccgca
      901 gggcggggtc attagtccgc tacttgctaa cttgttcctt cactatgcat ttgatttgtg
      961 gatggaaaga gaatatcggg gggtaccgtt tgagaggtac gctgacgata ttgtagtaca
     1021 ttgttcacga atgagtgatg taacaaggct aaagaacagg ttatcggagc gcttctcgga
     1081 agtcgggctg gtcctgaatg cagggaaaac gaatatcgcc tacattgaca cgtttaaaag
     1141 gagaaacgtc gcaacgagtt tcacctttct cggatatgac ttcaaagtgc gtacgctgaa
     1201 gaatttcaaa ggcgaacggt accgaaaatg catgccgggt gcgtcaaatg cagcaatgcg
     1261 caaaataaca gaaacaatca agaagtggcg tatacatcgc tcaacagctg agagtttgct
     1321 ggattttgcg aggcgctaca atgcgatagt gagaggctgg atcgagtact acggaaagtt
     1381 ctggtccaga aatttcaact atcgactgtg gagtgcaatg cagtcacgtc tgctcaagtg
     1441 gatgcagtct aaatacagac tttcgaaccg gaaggctcag cgaaagctga cgctggtaag
     1501 gaaggagtat ccgaagctat ttgttcactg gtatttacta cgtgcatcaa atgagtgatc
     1561 aagagccgta tgacgcgaga gtgtcacgta cggttccgtg agcagccggg gagtgaaatt
     1621 ctctccggct gactcgactc gcgggttccg acagcgatga cgaacatgcg gcggtgttgt
     1681 actcgctgat cggcacatgc cgtctgaaca atgtggaacc agaaaaatgg ctgcgttacg
     1741 tcattgagca tatccaggac tggccggcaa accgggtacg tgatctgttg ccctggaaag
     1801 ttgatctgag ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta
     1861 attctgctgc gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat
     1921 ttatcagttg tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtctggacgt
     1981 cctgttgctc cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag
     2041 ctattgcctg tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt
     2101 gtgccggaag caatattaca tcatggtcga gtttcctgat ttcccgggcc cagtaatgag
     2161 atgccccgcc agcctccatc gcaacagtca ttttaggctg ttgcacctgc cagttaagca
     2221 gttgattgcg agaaagtttg gtctttcctg cgggtttgcc atgagcattg atactgaaaa
     2281 gataaaagtt cgttttagcc agatcaatgc cggtgatttt ctgttcagac atggtatccc
     2341 gcttactttt gtggttaaag tttatccatc atggcgcaat tgaaggctgc cgtgaaagag
     2401 ggggaagact atctcatcgg ttactgttaa acggctaaga gaaactatct ttttcaagca
     2461 aagaagatac ggtgaaacag gcgatttcac cgtgaaatac tgttcaaaaa aaacgctaat
     2521 aaaagcaaaa tttcttttaa aacagccaat taaaaactgg tgagttgctg gcgaaaaact
     2581 tgaattttca cagcgaattc agattatctt ttagtacaag agcaatcata cgatctaact
     2641 tatcctgaac ttccaacggt aatctgccga attcatatga gaacgtacgt ccctttactc
     2701 gttttcgagc aaatttatct ttactatcaa atttccataa caaagttaca accgctttat
     2761 cctttactcc tgaatcaagc aatgaagaca tttccttggt tataagccgc ataatactgt
     2821 ttttaacctc gtcttcagaa agattgtcat cagcagtaat aagctcaata ctcggcgaga
     2881 tattcttcac aacttcatca atagatatat cacccagcct cagtcgttca cttaattcag
     2941 ccagttgcct gtaatcggcg tatgtcagct cagactgaac aggaaaaaga gacacaaaat
     3001 ctttaggtac ggacgctgct tggagggcac gagtcacctt agctgccgac aatccctctc
     3061 tttccgcaat ttgcttctgg ttcataccgg cctctttgag acgtactaaa cgcaacccaa
     3121 tttcacgaat attatgttca agtgacgtct gcaaatcttt cgccagatgc tgagcttcag
     3181 aaacagtaag ctcatcatcc gttacaagta cacgtaatcc aaccttgcat agcaaagctg
     3241 ctgcccgacg acgggaacca tccaaaatct caataagatc gccagtccga atcccaatac
     3301 atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc ttttagtgag tccggcgtca
     3361 gggctgtctg ttcgcggcca ttcacctctt caaccacaaa agtttgagtg tccactttgt
     3421 catgatcaac cttgatctca gtaaattttg cctttcggcc agtattgagg gtaaatacat
     3481 gaactggagc cgatatttct tcagtattat tcaggatatt tgtattcagc gtacggccaa
     3541 tagtcggtcg gtgctttctg ttttccatca gtagttggcc cttataaatt caatacgatc
     3601 aaaaactgct ttagcgaaat cttctgctgc catacgtgca tttttcaaag cttcaccact
     3661 gccaacataa gtaacaggat tcgcagatat cacggtatca aacgactcgc cgcttctctc
     3721 aaaaccatca agacgtggca ttgaaacatc aagcatgtca ccaccgaaaa tctcttttgt
     3781 caggctatgg cagtatttat gatctgactt attcgccagt ttcgacatga agccaatatt
     3841 tgcctgcaaa cggcatgaac atccatcctg ttcaataatc tgtacaagtt caggtaaacg
     3901 agccaaatat ttaagcgtgg aatggaaatc aacttgtgct ggcggcaccg gtgtaaacat
     3961 gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc agatgcgggc ctgtatcaat
     4021 aaggatgaag tcgaaatcat gcttcagctt atcaataata ttctcacgca atatcgcgtg
     4081 tttgttttgt cctaatagat gctcctcgca cagagtatcc cagtttgatg caataaaggc
     4141 gtcgtcaata gaagccggca taacataaac gccagggata actgacggaa cgataaaatc
     4201 ttccagtaat tcctcacgac tcacgttctg caacatagcc tgcggtgcgg ttgtatcaac
     4261 cagccccacc gcatgcaagt agttcagaaa catagtggct gatgactggg gatctaaatc
     4321 aaggagcaga atacgtaaat cttctgacag caggtgagga tgagcacgca gggcgtgagc
     4381 tacggaaaca ctactcacgg ttttagacac accgcctttt agactaccga tgaatatgga
     4441 atatgcctca ctataacgat cgcgatattt aggaatgcca cgatgtgcat agatatcgat
     4501 aatgttctgg atggttaatg catattttgt cgcggttcct gctgggcgtt tttcaaactg
     4561 atagccctga gcctccatct caccgactgc accatccacg cttgcgcgcg ttaattttgg
     4621 aagcttagct acagcagact ttgaatagac ttgataaaaa acatcagcct gaaactcctg
     4681 cttttgctcc tgaatctgtt ttgttagggc gagcaacatt ttatctgcac gctgcgcaac
     4741 cttacttaac tgctcaaaac tagtcattcc ccctccgtgc tatgtaggat tttaacttat
     4801 tacaaaaaaa actgcattag cagggtaatg catgcaagta catattgtac atgcttatat
     4861 taaaaatgcg cgcattgctg atttattaat catcggaatg aatattactg taagatagta
     4921 tgcctttccg ggagaaagac gctgatttcc cccgaataat ccagttcacc accacactcc
     4981 ggacaacagc tttcttcagg ctccagatgg tgtatttcgc gggaaacatg ttccggtaac
     5041 ggacggcgat gacgtgactg tcgcaacagg cgggggtagt gcttccaatc agtagaacat
     5101 gtgcttttca atgaaggagc cgatgacttt ttcgtggatt tccacggaac gcgagaagca
     5161 gattgtttta cgtgccagcc gcttgatgcg ggtgcagagg gtccggttgt tacgttcaat
     5221 acgttgcgtg aagattttgc cggtcaggtg tttctccttc ggcacttctc tggcgtagct
     5281 gccccagtca tcgctggtca ccatgccgat acagaacgga gtcaacaggg ctaacagttc
     5341 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     5401 gttgtacgca taccagagcc agtgttgtgt caacgacgga tgaaaagtgc tccacttata
     5461 tctccaccaa cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac
     5521 cccggccttt cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgacatgtga
     5581 gtggtgtaag atacggccca gcatcgctga ggtcagtgct gcatcaccgg cgaacgtttg
     5641 atcccactgc ccgaacggta gattggatgt caggatcatt gcgctctttt cgtaacgttt
     5701 agcgatgacc tggaagaaca gttttgcttc ttcctgactg aacggcagat agcctatttc
     5761 atcaatgatg agcaggcggg ggccattact ccacgctgaa gcgtcgtttt ataacggccc
     5821 tgacgttgtg ccgtagataa ctgaagtaac agatctgctg ctgttgtgaa gcgaactttg
     5881 atacctgcac ggactgcttc atagcccatc gctattgcca gatgggtttt ccccacacct
     5941 gatggcccca gtaatacgat attttcatta cgttctatga cgctgagtga gcgtaacgac
     6001 tggagttgct tctgcggtgc tccggtggcg aatgtgaagt catactcttc gaacgttttc
     6061 accgccggga aggctgccat tcgggtatac atcgcctgtt tacgttgatg acgtgccagt
     6121 ttttcttcat gaagcagatg ctccaggaag tccatataac tccattcctg gtctactgcc
     6181 tgttgtgaca gcgcaggcgc tgcgcttata aggctttcca gttgcaactg cccggcgagc
     6241 gccatcagtc gttgatgttg cagttccatc atcacgccac tcctctgcag aatgagtcgt
     6301 agatggagag tggatgatgc tgg
//
LOCUS       DAJTYI010000181         6281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYI010000181 DAJTYI010000000
VERSION     DAJTYI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..3228
                     /locus_tag="OO564_003708"
                     /pseudo
     CDS             1..3228
                     /locus_tag="OO564_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3238..>6281
                     /locus_tag="OO564_003709"
     CDS             3238..>6281
                     /locus_tag="OO564_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654652.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLAD"
BASE COUNT         1677 a         1648 c         1517 g         1439 t
ORIGIN      
        1 gtaataaaac agccacattt gacttcagca cctgttcttt agaatggcaa aacaccgtcg
       61 cccaagcgat cagtcagatc gacggcctga aaacaacaca gctaccatca ccagtaatgg
      121 ctgtactcac ggcacttgaa atgaaatgca caagatataa agtgcgtgag gatgttatgg
      181 atcagatcgt ccaggaaggc ggtctggaat atgctactga tgtaataata caccttcaac
      241 agattgatat taaatgggat tatgcgaata atgtcattat tattctgccg tctggcattg
      301 cacctgacta cttggagcaa tattccagat ttgaattacg cctacgtaaa catttatcac
      361 tggcggaaga gtctctctgg caaaaatgtg cacaaaaact tattgccgca attccacata
      421 ttccagaatg gcggcaacca ttaattgctt tgttattacc cgaaaaacca gaaattgcac
      481 atgaaattgc ccagcgtcta ctggggcaaa aaaaattacc ctcgcttgag tggttaaaaa
      541 tagtggcgac tgatgagcac attcttgcct cattagaaaa atatcacgag ccatatgcca
      601 tttttgatga ttactattgt ggtgcgatat ggtcagccac cgtattacag gagcaaggtg
      661 ttgcagccct gccccgattt gctccctatg ccgcaagtga ctactgcgcc gatgtgttgc
      721 gtcatatcaa tcatccgttc gcattaacac tgcttatacg tgtagccggg catactaaac
      781 gctgtcacga tcgaatgacg aaagcctgcg ctgcgttccc acacgcagca ctggcagaac
      841 aggtcattcc ctggctttcg acacccgcag ttgcagtgct gaaatcatgc cagcaccaac
      901 tgacacagcc ctcaaaccat gccagcgccg atctactgcc agccatagta gtctccccac
      961 catggatttc gaaaaagaaa aaatcgccga ttccggtgct ggatttagcg ccattaggca
     1021 tcgagccaat ctgttatctg acagaagaaa tcagtaatca acttttggcg aaatatatct
     1081 ggtattcaaa acacatcacg gttagccatg aagaaagtac taccaacctg ttggcaagga
     1141 tgggttttca acgacggatc gctggtacat atattaaagc tcccgaagcg gtagttgagg
     1201 catggctaaa tgaagattat tcaaccttac taagtgaatt taaggtgttt cattcaccta
     1261 ccgggcatta ttggcagttg gggattttga caacattgcc gctggaggct ggagaaagca
     1321 gtaaaagcat ggaatgccct tactctatct ccacataccg ataccgaata cgccatgtta
     1381 cattttggac tcaaaggttt acctggttta gttaactcaa ttacacgcta tccgcaggaa
     1441 gccttgccca tcacgaatta cttcgcagcg agtgagctgg ctcctgccgt cgcccgtgcc
     1501 ttcaacaaac tcaaaacgct acgcgaaaac gcccgcacct ggctgttgaa atacccggaa
     1561 catgccctta ccggcctgct gcctgcggcg ctcggcaaag ccggtgaagc acaagataac
     1621 gcccgcgctg ccttgcgtat gcttaccgaa aacggtcatc agccattact gcaagaaatc
     1681 gcccggcgtt ataaccagtc ggaagtaaca gacgcggtga acgctctgct tgcgctcgat
     1741 cccttagata atcacccgac aaaaatcccc actcttccga ccttttatca gccatcgatc
     1801 tggacgcgcc cggtattaaa agcaaacgcc caatcactgc cagataacgc cctcctccac
     1861 ctcggcgaaa tgctccgctt ccctcaggaa gaggctctgt atccgggatt attgcaggtg
     1921 aaagacgcct gtaccgctga ctctctggct gaatttgcct gggatctgtt taccgcctgg
     1981 cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca ctgcgttagg cgttctcggt
     2041 aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg cctggcctgg tgaatcccag
     2101 cataaacgcg ccaccgttgg gttggatatt ctcgccgcta tcggtagtga tatcgccctg
     2161 atgcagctta acggcattgc tcagaaactg aaattcaaag cattacagga gcgggcaaaa
     2221 gaaaagattg ccgacattgc cgagagccgc gaactcacgg tagcggagct tgaagatcgg
     2281 ttagcaccgg atctcggtct ggatgataac ggttcgttgc tgctggattt tggcccacgc
     2341 cagttcaccg tcagctttga tgaaacctta aaaccgtttg tgcgtgatgt ttccggcagc
     2401 cgcctgaaag acctgccaaa accaaacaaa agcgatgatg agtcacaggc gaacgatgct
     2461 gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg tcgccgccca gcaggtagca
     2521 aggcttgaat ccgccatgtg cctgcgtcgc cgctggtcgc cggaaaactt ccaactcttc
     2581 ctggttgtgc atccgctggt tcgccactta acccgccgtc tgatttgggg cgtttatagc
     2641 gccgacaacc agctactggc ctgtttccgg gtagcggaag ataacagcta cagcaccgct
     2701 gacgatgatc ttttcaccct gccggaaggc gatatctcta tcggcattcc tcacgttctg
     2761 gaaatatcac cgacggatgc tgtcgccttt ggtcagcttt ttgccgacta cgaactgcta
     2821 ccaccgttcc gtcagcttga ccgtaacagt tacgccctga cagaagccga gcgcaacgcc
     2881 agcgaactga cccgctgggc aggcagaaaa tgcccgagcg gccgggtcat ggggctggca
     2941 aataaaggct ggataaaggg cgaaccacag gatggaggct ggatcggctg gatgatcaaa
     3001 cctctcggtc gctggtcgtt aatcatggaa atcgacgaag gttttgcggt gggtatgtca
     3061 ccagccgaac ttagcgctga gcagctctta agcaagctgt ggctatggga aggcaaagcg
     3121 gaaagctatg gctgggggag taattcaaca caggaagcgc agttctccgt actcgatgcc
     3181 atcaccgcca gcgagctaat taacgatatt gaagccctgt ttgaataagg aaagagcatg
     3241 gacaaggaat taccgtggct ggcggataac gcccaactgg aactgaaata taaaaaaggc
     3301 aaaacaccac tcagtcatcg caattggccg ggcgaaccag tgcctgttat cactgaaagc
     3361 atcattcaga cactgggtga tgaattgcta caaaaagctg agaagaaaaa aaacattgtc
     3421 tggcgttatg aaaatttttc accggagtgg cagtccgcca ttacgcaggc catcaacttg
     3481 atcggcgaac acaaaccctc agtcccggcc cgcacaatgg cggcactagc ctttatcgcg
     3541 caaaatgaca accaacagtt gctcgacgaa atcgtccaac aagaggggct ggaatatgcg
     3601 actgaggtgg tgattgcacg ccagtttatt gcacggtgtt atgagagtga tcctctggta
     3661 gtcacattgc agtatcagaa cgaggactat ggctatggtt atcgctcaga aacctataac
     3721 gaattcgatc tccgactgcg taaacacctc tctctggcag aggaaagctg ctggcagcgt
     3781 tgcgccgaca aactcattgc cgcactgcca ggaataacca aagttcgccg cccttttatt
     3841 gcgctgatcc tccccgaaaa accagaaatc gccaatgagt tggtaagcct tgaatgcccg
     3901 cggactcatt ttcattctaa ggagtggtta aaagttgttg ctactgaccc caaagcggtg
     3961 agaaaactcg aacgctactg gagccaggat atatttagcg atcgagaagc cagctacatg
     4021 tcgcatgaaa accacttcgg ctacgcggcc tgcgctgccc ttttgcgcga acaaggactg
     4081 gcagtcgttc cgcgcctcgc gatatatgcc cataaagaag attgcggcag tctgctggta
     4141 caaattaacc atccgcaagt catccgcacc ttgctgttag ttgctgataa aaacaaaccc
     4201 agcctgcaac gtgtggctaa atacagtaaa aacttccccc atgcgacact cgccgcactg
     4261 gcagaactgc tggcgttaaa agaaccacca gcccgcccaa gttatccaat catcgaagac
     4321 aaaaagctgc ctgcacagca aaaagctcga gatgaatact ggcgtacgct gttacaaacg
     4381 ctgatggcat cgcagccaca actggcagca gaagtgatgc cgtggttaag tactcaagcc
     4441 cgggcagtgc tgaatagtta tttatcggca ccgcccaaac cagttattga tagcaccgat
     4501 aacagccaaa tgcctgaaat cctcgtttca ccaccatggc gtgggagaaa gaaaaagact
     4561 tccatacgtc acttttcgct gcctgatctt acgcttgcac cgcaaacgta caggtcgccg
     4621 aataaacact ggacgccgga acagcaaact gcaattgacc attttagcgc catgtctttt
     4681 gacgaacgtc ttgctacgag aggagcggat atcttcttgc gggaattagg ttttgacgtt
     4741 catagcctgg aatttaataa gcgtcttctt aatggacagt tggatgcctt tgatgctcag
     4801 caactgcaag aatacacatc aataggacta ttgcgtactt atcacgcgca tcaggccaaa
     4861 tctaaacagt accgggaaga cgccaccaat gcattactgg cacaagatag cattgcgttg
     4921 atgggagggt ggcgaacttt tcagcacgga ttttactatg gtaatgaaca gcgttggggg
     4981 atttggaatc tgtaccttat cgcgcagatg ccgcgcgaaa tggcggtatc gtgctggcaa
     5041 caaattgtcg aggctgattt tcattacacc ggggtggaat atttgttaag tgtgctgggt
     5101 accgatgctt tgcctggatt aaacaccgcc tttgcccgac atccaaaaga gatatttccg
     5161 ctgctgattc atttcggcgc aacagaactg gccctgccca ttgcccgcgt ctggcacctt
     5221 tttgcgggcc agcgtaatct ggctcgccag tggattttac aatggccgga acatacggct
     5281 actgcactta tccctcttgt cttcaccaaa tctagcgata aaagcgaagc tgcattactt
     5341 gccctgcgtt tactttacga acatgggcat ggcgaattgc tacaaactgt agccaatcgt
     5401 tggcagcgta cagatctatg gcctgccctg gaacaattac ttaaacagag tccaatagag
     5461 atttacccgg cacgcattcc aaaagcccct gatttctggc atccgcaaat gtggtccagg
     5521 ccgcgcctta tcactaataa tcaacctgtt accgatgacg ctctggaaat tatcggcgaa
     5581 atgctgcgct ttacccaggg gggacgtttt tatagcgggc tggaacaact gaaaacgttc
     5641 tgccagccac aaacgctggc agcttttgcc tgggatctct tcactgcgtg gcaacaagct
     5701 ggtgcccccg caaaagacaa ctgggcattt ctggcgttaa gtctctttgg tgacgaaagc
     5761 acggcacggg atctaacgac acatatcctc gcctggccac aagaaggcaa atctgcccgt
     5821 gcggtcagtg gcctgaacat ccttaccctg atgaataatg atatggcgct gatgcagctg
     5881 catcatatat cgcaacgggc gaaatcccgc cccttacgtg ataacgcggc ggaatttctt
     5941 caggtagtcg cagaaaatcg cgggctaagc caggaagagt tagcggacag attagtccca
     6001 acactgggcc ttaatgatcc gcaggcgttg agttttgatt ttggtccccg gcagtttacc
     6061 gttcgcttcg atgaaaacct caacccggtt atctttgatc agcaaaacgt tcgccagaaa
     6121 agtgttcccc ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc actggcccga
     6181 ctaaaagggt taaaaaaaga tgctactcag gtgagcaaaa acctgctccc gcgtcttgaa
     6241 gctgccctac gtaccacccg acgctggtcg ctggcagatt t
//
LOCUS       DAJTYI010000182         6228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYI010000182 DAJTYI010000000
VERSION     DAJTYI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1286
                     /gene="sbcB"
                     /locus_tag="OO564_003710"
     CDS             <1..1286
                     /gene="sbcB"
                     /locus_tag="OO564_003710"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6654653.1"
                     /translation="FYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLF
                     TVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPE
                     GINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYL
                     FTHRNKHKLMALIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAG
                     DISPLLELDIDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLG
                     INRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKI
                     VLETEPRNLPALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFL
                     QGYADELQMLVQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1329..1556)
                     /locus_tag="OO564_003711"
     CDS             complement(1329..1556)
                     /locus_tag="OO564_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6654654.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1570..2628)
                     /gene="yeeE"
                     /locus_tag="OO564_003712"
     CDS             complement(1570..2628)
                     /gene="yeeE"
                     /locus_tag="OO564_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6654655.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2807..4165)
                     /gene="plaP"
                     /locus_tag="OO564_003713"
     CDS             complement(2807..4165)
                     /gene="plaP"
                     /locus_tag="OO564_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6654656.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4155..4235)
                     /gene="yoeI"
                     /locus_tag="OO564_003714"
     CDS             complement(4155..4235)
                     /gene="yoeI"
                     /locus_tag="OO564_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6654657.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4433..5362)
                     /locus_tag="OO564_003715"
     CDS             complement(4433..5362)
                     /locus_tag="OO564_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6654658.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5408..>6228)
                     /locus_tag="OO564_003716"
     CDS             complement(5408..>6228)
                     /locus_tag="OO564_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6654659.1"
                     /translation="KVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSGID
                     SYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAHRI
                     PRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLAGL
                     VGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHPAR
                     NVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
BASE COUNT         1676 a         1643 c         1488 g         1421 t
ORIGIN      
        1 tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta ttaattaccg
       61 gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
      121 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
      181 acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct
      241 ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc
      301 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
      361 atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt
      421 acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg tttgattatc
      481 tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
      541 ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc tgggtggcac
      601 cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg gcaggagaca
      661 tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat accgcaaaag
      721 ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
      781 tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
      841 agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg
      901 tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt
      961 ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc
     1021 cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa aagttgttgt
     1081 tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc
     1141 aggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct gatgaattgc
     1201 agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta aaagcacttt
     1261 ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg gcggaggaca
     1321 atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa
     1381 tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggta
     1441 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
     1501 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
     1561 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
     1621 cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg
     1681 ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact
     1741 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
     1801 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgccccca cgaagatccc
     1861 taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag
     1921 aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg cttcactcag
     1981 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
     2041 tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag
     2101 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggtaac
     2161 caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg
     2221 ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat
     2281 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
     2341 ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt
     2401 accaagccac gggaacgccc cggcttcgta agtcagtagg cccgccagaa ttaacgcaaa
     2461 aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc gattattttt
     2521 cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa
     2581 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
     2641 cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat
     2701 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc
     2761 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
     2821 cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga
     2881 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
     2941 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
     3001 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg
     3061 ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca
     3121 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
     3181 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
     3241 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
     3301 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
     3361 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
     3421 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
     3481 ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
     3541 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc
     3601 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
     3661 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca
     3721 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     3781 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     3841 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     3901 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     3961 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     4021 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     4081 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     4141 tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa
     4201 gcaaaaaatt tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
     4261 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
     4321 taaccgacgc taattaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct
     4381 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg
     4441 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
     4501 catctcgcca ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacag
     4561 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
     4621 gttgcgccag ataccgtggt aaatagccgc agccaaggcc gctaatttgc agctccagtt
     4681 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
     4741 aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac
     4801 ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
     4861 caagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
     4921 caacgataat atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat
     4981 tgataaactt caggcgcgtc acgctatgat gttgataaaa agcttcaatg agaggcgcaa
     5041 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
     5101 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccctttct
     5161 ctaataacat ttttccggtg cgggtgaatt tagcgcggtg tccgctacga tcaagcaatt
     5221 gaatatttag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
     5281 agagtttggc tgacgccgcc gcaaaactgc cctctttttc tatggcatcg aggatcatca
     5341 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
     5401 tgatcgttta ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt
     5461 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
     5521 actgcggagg ttgcagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
     5581 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
     5641 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcagggg
     5701 cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg tccgaccagg cccgcaagac
     5761 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
     5821 ctcgtccact gttggttacc ggattacgcg gggtagtttc tttaaccgtg ccttgcgcgt
     5881 cgccatagac agatgttgag ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc
     5941 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg
     6001 ccggaagcgt aatgaccagc gcatcggcat ccataagggc atccagatcg tcagaatcgc
     6061 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
     6121 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
     6181 tcgccagcgg catacctaac caccctaacc cgacaattgc gacctttt
//
LOCUS       DAJTYI010000183         6102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYI010000183 DAJTYI010000000
VERSION     DAJTYI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..648)
                     /gene="istA"
                     /locus_tag="OO564_003717"
     CDS             complement(<1..648)
                     /gene="istA"
                     /locus_tag="OO564_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6654660.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG"
     gene            complement(735..1096)
                     /locus_tag="OO564_003718"
                     /pseudo
     CDS             complement(735..1096)
                     /locus_tag="OO564_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1436..1990)
                     /locus_tag="OO564_003719"
                     /pseudo
     CDS             complement(1436..1990)
                     /locus_tag="OO564_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2133..2255
                     /locus_tag="OO564_003720"
                     /pseudo
     CDS             2133..2255
                     /locus_tag="OO564_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2517..3083)
                     /gene="ipgB2"
                     /locus_tag="OO564_003721"
     CDS             complement(2517..3083)
                     /gene="ipgB2"
                     /locus_tag="OO564_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6654661.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3365..3679)
                     /locus_tag="OO564_003722"
     CDS             complement(3365..3679)
                     /locus_tag="OO564_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654662.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3757..3867)
                     /locus_tag="OO564_003723"
                     /pseudo
     CDS             complement(3757..3867)
                     /locus_tag="OO564_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4191..4868)
                     /gene="ospD1"
                     /locus_tag="OO564_003724"
     CDS             complement(4191..4868)
                     /gene="ospD1"
                     /locus_tag="OO564_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6654663.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5239..5376)
                     /locus_tag="OO564_003725"
                     /pseudo
     CDS             complement(5239..5376)
                     /locus_tag="OO564_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5458..5756)
                     /locus_tag="OO564_003726"
                     /pseudo
     CDS             complement(5458..5756)
                     /locus_tag="OO564_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1639 a         1350 c         1266 g         1847 t
ORIGIN      
        1 gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac cggtctgata
       61 tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc gcggcacacc
      121 accaaagaag cggaacgcat tatgatggca ggtctccagc gtgtcataac gcatattgtc
      181 agtgaattcg atgtacaaca ttcggctgta tccgggaaca gcaacgaaca cgtgaagcgg
      241 tgagcgacca ttacgcatag tgccccagtc aacctgcatc tgtcgtccgg gttcagtttc
      301 gaaccgaacg gcaggctcct gctcctgagg aaccgagaga gaacgaatga atgccctgag
      361 aatggtcatt ccgccacgat atccctggtc tctgatctcg cgagcgatta ccgttgccgg
      421 gattttgtaa ggatgagcat cggcgatgcg ttgacgaata taatcccggt attcatccag
      481 gagtgaagca acagcaggtc gcggcgtata ttttggcggc tcagattttg cctgcaaata
      541 acgtttaacg gtattgcggg agagccccag ttctctggca atcgcccggc tactcattcc
      601 ctgcttgtgc aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga
      661 atcaggagag cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg
      721 caccgttcat gacagtgttg ttgcgccttg ctgccaacga agctccactg cccatcaagt
      781 tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg ccggggcggg
      841 agctttttaa tgtccgtatg acggtattga tgccgatttt cagcgtacgg gcagtatcgc
      901 gaactccggc accgttgaaa gccatttcag tgatttggtt tttaactccc ggtttacggg
      961 cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc acggcaacgg aatcgatcat
     1021 gtccttttgg gttctgcccg tgacggtaaa cctgaacgga ctggcaacgg gggcaatgaa
     1081 agttaactgt gaccatgaaa gaaccttaaa agcgggtatt ataaccccta attcaactaa
     1141 ttggaggcat tgtcattttt aatctatgga tacacttaaa aatcctctca ccttacgtta
     1201 aaaaccttct catgtttaga gttttttatt atgtttatca taatgatatg acgatctgag
     1261 tttttaaaat caagaagaaa agataagttt tttaaatgca cagtgcctgt ggataacttt
     1321 gcgtaaatcc ggaggacaga tcactctgaa gcaagacaac agcatctgaa aacaatatgc
     1381 tcacgtttca accacagggc gtaagtgatc ctacccacgt aatatggaca caggcctaag
     1441 cgaggttctg gttttcaaat tgttccggac tgaggccgcc acaccaactg tgccgtcgcc
     1501 agcgattgta atcacattcg atataattaa acactgttgc ccgcattatt tcccggctga
     1561 taaagtgttc tccgtggaga cattccactt tcagcgaatg aaagaagctt tccacgcagg
     1621 cattatcgta gcagcagccc tttgcgctca tactgccacg cagattatgc cacttcagca
     1681 gcgcctgata atctgctgaa cagtactgac ttcctcggtc cgagtgaacg ataacgttcc
     1741 ggggtctctt acgccgccac agcgccatct gcagggcatc gcaggccagt tgcgccgtca
     1801 ggcgtggtga catggaccag ccaataacgg cacgtgacca caggtcaatg accacagcca
     1861 gatacagcca gccttcatct gtacgtaagt acgtgatgtc tcccgcccac ttctggttcg
     1921 gaccactggc gtaaaaatcc tgctccagca gattttctga cacaggcagg ccatgtgcgc
     1981 ggtaactgac cgtattgctg ggtaagatca atttgctcac tggtatttac tacgtgcatc
     2041 gaatgagtga tcaagagccg tatgacgcga gagtgtcacg tacggttccg taagcagccg
     2101 gagagtgaaa ctctctccgg ctgactcgac tcgcaggttc cgacagcggt gacgaacatg
     2161 cagcggtgtt gttctcgctg atcggcacat gccgtctgaa caatgtggag ccagaaaaat
     2221 ggctgcgtta cgttattgaa catatccagg actgaccggc aaaccgggta cgcgatctgt
     2281 tgccttggaa agttgatctg acttctcagt aaatatcaat atgattctga cgagccgctt
     2341 acctttgagc agagaaaggg gagagggatc ccacatccat gagctaggaa aatacaaaaa
     2401 ctggctatac aacaattcaa tatattagga cttacttgcg aggagaaata tagcaccgct
     2461 caggcaattc ctgggtattt acttttctta gtttctttac tcgggatgtt ctgtcatcag
     2521 aaaggcgatt ctaaatttgt aatatagtca ctcatattcc cacgtaattt ctttagatca
     2581 atatattgca catttttctc aaaaaactga tcggaaataa gatttgcaac attgcttgtt
     2641 gtatcatttt taacgcttct attcatccct tcacaaagtt cgtccatttt ctttttgaaa
     2701 taactgcttc ctgatacaag atgatttatg gaactttgtg cagcattgat gtctagagaa
     2761 atgcgataat ctttctcaac ctgtttaaat atctgtttac ggagattatc gccgattttt
     2821 cggctgtgtt ctgcacaaaa cttatcaata cattggttca ccacccggga tacgtagact
     2881 tttttttcat catttttcca atctgtaata ttttcaccga tttgttctga aacagagctc
     2941 aaaatactag tatttatttt ggttgatgat atttttacta tccttttccc cattgtacag
     3001 cggataatct catccacctt tcctgaaaaa tatcttttgt gagataaact gattccaaaa
     3061 ttattaaaag atgttccaag cattataatc actttgtcat gtaaaatagt aacattgtca
     3121 ttattttagc tgattcaaac aattctgtaa atgctttgag ttctttactc ccttggctca
     3181 atgatggacc gtctggaatc ccagagacta aactctctga gaaagagagt atgaaaaaac
     3241 ttggggcagt ccctaaatga agggaccaac agcggtaaaa taaccggcat cattcataat
     3301 gatgccctgt tagttgtagt tatgaagatt tacgtcatta aaattgctgt tcgtagtgtc
     3361 agtcctatgg tctggcatca gctgaggatc gtagccgata catcactggc ttcacgacat
     3421 tcgtgttgag gctattcatg aaaactccac gctgaaaacg ccgttttgta taaacggtca
     3481 cggtatgccc ggagccactg ttgctgatga attcgataaa accttggcgt ttcttgaagc
     3541 tatcgttaat gcggataatg aaacaacgat tggtgagatt cgctcttttg ccgacgccct
     3601 ggacgctgtc cggttcaatc gcaacaaaat caaccggcag ctgagcaaac ccaaccttgc
     3661 ctctctggcg ctggagcatg aagtgatttg gctgggacgt agccgctgac agcttctata
     3721 aaagtatgat cctacccacg taatatgaac acaggcttaa gtgaggttct ggttttcaaa
     3781 ttgttcagga ctgagacctc cacaccaact gtgccgtcgt cagcgattgt aatcgcactt
     3841 gatataatta aacactgtat atttcatttg tggagtctcc agtctgaggg ggctttatgc
     3901 attccgtatt gggcactatg atgccagaaa tctgcaaggc tccacacctt cctgtatttc
     3961 ccttcctcta tcacagtgca ctttacttaa tagggtggga tgtgttcatt acatttattg
     4021 tgttttttat gattgttttt gcgtccagtg tcgttcgtca tgggaaactg gttctatggg
     4081 tagtatcgct cagtctagtt aactgtttgt ttctttttag aaattaagca gatcacacaa
     4141 ttcggggaga gatccttcta agtgcaattc tttgcagcta tttagcacac ttaaaacaac
     4201 tcttgtattt tgtcagaaat aatattaaac atgtcttgtt cattattgtt aagtctgtct
     4261 gctatttctg taagttttat cccactatga tggcatatat ttatatatgc catcatagcc
     4321 tcgatattcc ctttccctgc ggccagaaat aatcctggta acccctcatt atctcttaca
     4381 tccaatagtt taatcgtttc ttttggtgat aaatcaaaag ttttaataaa atctccataa
     4441 gcttgaatcg cagcggaatg accttcttga agagctatga acaaagcaga ttccccagca
     4501 ttatttcgtg ctgcaagaac ttctagtttt tcctctttgg gtatttcggc tgccaacagc
     4561 gattcaccgt atttacgcgc tgcatcggca tttccatgcc aaatcaatga aaacaaatta
     4621 tggcggttat actccttatt attctttgtc ggaatggata tgctctgtat tttggccaat
     4681 tttctgaaag aaacaccact atatcccccc cctgattttt cttcattgat ggcgtgggat
     4741 atagcggtat ttgtcacgtt aatgatatta tttttcattc ctttattttc attagcagta
     4801 ttgctgccta ttattaggtg catatttttg ttgtttgctg gatgtaatcc atagttattt
     4861 attgacattt ttgttcatcc tttttgctaa aaaaaacaat aacctatttt tgtaatgtaa
     4921 tagatattag tctattgcta atctaaaaaa accattgcca cattgaatgg ctatattcac
     4981 caataaattc tttcgatttc gtatattgac atatcgtaga tatatcttat ccctaagatt
     5041 caataatcga gtccgtttac cattctgtgc aagcgccttc actcagaagt gaccgtcaag
     5101 gcgatcacca aacttgataa taaagcagct cattgccata cgccagtcct taaggggcgt
     5161 tgtccatttc tgtgatgctg cctagattta actgccagct ccggcttatc tacatctaaa
     5221 tatgtgagcc taataccgct acactttgtc cagactatgt ttttctccgg gaagtgggct
     5281 aaccgtctcc ataaagtgac gaactttttt caacagtggc catattgaac ggcactgatg
     5341 attacgggtt attgtctcat gaagagccgg ccacatcact gacagcagtg acttcaggcc
     5401 ttcagcacca gacagcctga accagttaat ccatcgcccg acagatttag taatctaggg
     5461 ctggtttttc ttcagctgtt gcggttcgaa agtgccatta cgatcacgca gagtacggat
     5521 ttccagaggt tcatctccag tagtgataat cttcgttaag caaccattgc gggaattgct
     5581 gccagatcta agctgatttt tctcgcaccc aagatgatga gtcatattag cattgagtac
     5641 ggtttcattt tcagcatcca atcaaactga ctgagatctt caggattttt gagacttttg
     5701 gctagttcat tagctaaagc ccgcaactta ttagatgtca actgtttttc attcataaat
     5761 tcaccttcat ttgatatcaa attaaatagt tcaacatcag agaattatac aaacttatgt
     5821 acaggctcct atgatcttcg aatcctgaag attatagtga aaataagttt tcacgttgaa
     5881 atcccacata gttagtatat gataaataat catggatgac ctgattaacc tcttggggta
     5941 gtgcttttat agaagaataa atattctact aatttaatac atcacctaaa atagtattgt
     6001 tttttatggt tggttgaagg tcgtggtgat gatgttagtc cttttgagaa tattccagtg
     6061 ccagatctta cttataataa agaggcttgg cagtttggat aa
//
LOCUS       DAJTYI010000184         6068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYI010000184 DAJTYI010000000
VERSION     DAJTYI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1954
                     /locus_tag="OO564_003727"
     CDS             <1..1954
                     /locus_tag="OO564_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6654664.1"
                     /translation="YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTY
                     TAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIH
                     LALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIG
                     LTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQ
                     TDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHA
                     ERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTT
                     GVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERF
                     DGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGP
                     FPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDF
                     TRKWQDADRKEALIHELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAE
                     NVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTG
                     FGDRNGYNQALSELENEIYQLPPRSA"
     gene            2021..3481
                     /locus_tag="OO564_003728"
     CDS             2021..3481
                     /locus_tag="OO564_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6654665.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            3490..5151
                     /locus_tag="OO564_003729"
     CDS             3490..5151
                     /locus_tag="OO564_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6654666.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5372..5713
                     /gene="symE"
                     /locus_tag="OO564_003730"
     CDS             5372..5713
                     /gene="symE"
                     /locus_tag="OO564_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6654667.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(5760..>6068)
                     /locus_tag="OO564_003731"
     CDS             complement(5760..>6068)
                     /locus_tag="OO564_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654668.1"
                     /translation="HLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSL
                     NMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV
                     "
BASE COUNT         1786 a         1442 c         1394 g         1446 t
ORIGIN      
        1 ctattacgac gatggcagcg gcaaatcgcc tcgttattat caacttcagg caatcaacaa
       61 aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg ctggtcatgg cgaccggaac
      121 agggaaaacc tataccgcat tccagatcat ctggcgcctg tggaaatcaa aaaataaaaa
      181 acgcattttg ttccttgccg atcgcaacat tctggtcgac caaaccaaaa ataatgattt
      241 ccagccattt ggtacggcaa tgaccaaagt cagcggacgc accattgatc ccgcttatga
      301 aattcacctc gcgctctatc aggctataac tggcccggag gaagaccaaa aagcgtttaa
      361 acaagtcgca ccagatttct tcgatctgat cgtgatcgac gaatgccatc gcggcagcgc
      421 atccgaagac agcgcctggc gagagatcct tgattatttc agttccgcca cccaaattgg
      481 cttaaccgcc acgccaaaag agacgcatga agtctccagc acggattact tcggcgatcc
      541 ggtttacgtc tactcactaa aagaagggat cgaagacggc ttcctcgctc cttataaagt
      601 tgtccgtgtt gatattgatg tcgacctgca aggctggcgc ccaaccaaag ggcaaactga
      661 cttaaacggc gaagtgattg acgatcgtat ctataaccag aaagatttcg atcgcacgat
      721 ggtgatcgac gaacgcacag aactggttgc cagaaccatt accgactacc tcaagcgcac
      781 caatccgatg gataaaacca tcgtcttctg taacgacatc gatcatgcag aacgtatgcg
      841 ccgcgccctg gttaatctca acccgcagca ggtgaaaaag aacgacaaat acgtcatgaa
      901 aatcaccggc gatgatgaaa ttggcaaagc tcagttggat aacttcatca acccgaaaaa
      961 accgtacccg gttatcgcga ccacttcaga gctgatgacc actggtgtgg atgctaaaac
     1021 ctgcaaactg gtagtactag accagaacat ccagtcgatg accaaattca agcaaattat
     1081 cggtcgtggt acacgcatcg acgaacgtta cggcaaactc tggtttacca tcctcgactt
     1141 taaaaaagcc accgaactgt ttgccgatga gcgtttcgat ggcattcccg aaaaagtcat
     1201 ggataccaca ccagaggata tcgccgatcc agaatctgat tttgaagaga aactcgaaga
     1261 aatcagcgaa catgacgacg aacaggtaac aggcgttgat gaaccgcctg cgccaccata
     1321 ccaggttacc gataccgatg atgtcggccc atttccggaa gaagacgaga agaaaatccg
     1381 caagtttcac gtcaacggtg tagcggtggg cgttattgca cagcgcgttc agtattacga
     1441 cgccgacggt aaactggtta ccgaatcctt taaagattac acccgcaaaa cactgctcaa
     1501 agaatatgcc tcgctggatg actttacccg caagtggcag gacgccgatc gcaaagaagc
     1561 gctcattcac gagctggagc aacaggggat catctgggaa gtactggcag aagaagtcgg
     1621 taaagatctc gacccgttcg acattctttg ccacgtagtg tatggtcagc cgccgttaac
     1681 ccgcaaagag cgcgccgaga acgtgcacaa gcggaactac ttcacaaaat actctgaagc
     1741 agcgcaagcc gtgctcgata atctgctgga taaatacgcc gatgcgggcg tacaggagat
     1801 cgaaagtatt caggtgctga aacttaaacc attcgacagc atgggcacct taccggagat
     1861 tattaaaacc ggatttggcg accgtaacgg gtataatcag gcgctcagcg agctggaaaa
     1921 cgaaatctac caattaccgc cccgctctgc ttaacaactc tcttttgcgc cttctgcaac
     1981 catcatgttg cagaaggcct tgtttttcat ggaatacaac atgtctatca gctcagtaat
     2041 caaatcatta caagatatta tgcgcaaaga tgccggtgtg gacggcgatg cgcagcgtct
     2101 cggtcagctc tcctggctgc tgtttttgaa aatcttcgat gcccaggaag aggcgctgga
     2161 actggagcag gataactatc aatatccgat cccacagcgt tatttgtggc gcagttgggc
     2221 cgccaacgcg cagggcatta ccggtgattc cctgctggaa ttcgttaatg atgatctgtt
     2281 tccggcgttg aaaaacctca ctgcgcctat cgataaaaac ccacgcggct acgtggtgaa
     2341 gcaggcgttc agcgatgcct ataactatat gaaaaacggt acgctactgc gccaggtgat
     2401 caacaagctg aacgaaattg actttaccag cgccagcgaa cgccatctgt ttggtgatat
     2461 ttacgaacag atccttaaag atctgcaatc tgcgggcaat gcgggcgaat tctatactcc
     2521 acgcgccgtc actcgcttta tggtggatcg cgttgatccg aaactcggcg aatccattat
     2581 ggacccggcc tgcggtacgg gcggttttct tgcctgcgcg ttcgatcatg taaagaacaa
     2641 atacgtgaag agcgtcgccg atcatcagac actgcaacaa cagatccacg gtgttgagaa
     2701 aaaacagctt ccgcacctgc tggcgaccac caatatgctg ctgcacggca ttgaagtacc
     2761 ggtacaaatt cgtcacgaca acaccctgaa taaaccgctt tcctcctggg atgagcaact
     2821 ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa gaagacggta ttgagaagaa
     2881 ctttccggca gagatgcaaa cccgcgaaac ggcggatctg ttcctgcaac tgattgtgga
     2941 agtactggcg aaaaacggtc gtgcggcggt ggtattgccg gatggcacac tatttggcga
     3001 aggtgttaaa accaaaatca aaaagctact taccgaagag tgcaacctgc ataccatcgt
     3061 gcgtttaccg aatggtgtgt ttaatcccta taccggcatt aaaaccaacc tgttgttctt
     3121 taccaaaggt cagccaacca aagagatttg gttctatgaa catccgtatc cggcgggcgt
     3181 gaaaaactac agcaaaacca agccgatgaa gtttgaagag tttcaggcgg agatcgactg
     3241 gtggggtaac gaggccgatg gttttgccag ccgcgtcgag aatgagcaag cgtggaaagt
     3301 cagcattgat gacgtgattg cacgcaactt caatctggat attaaaaacc cacatcaggc
     3361 ggaaaccgtc agccatgatc cggacgaact gttagcgcag tatgcaaaac agcaggcgga
     3421 gatccagacg ctgcgtaatc aactgcgcga tattcttggc gctgcgctgt ctggcaagta
     3481 ggttaactaa tgagtgtgga aaagctgatc gttgatcata tggaaacctg gacctcggcg
     3541 ttgcaaaccc gttccaccgc cgggcgcggc agctccggta agattgattt gtatggtatt
     3601 aagaaattgc gtgagctgat tctggaactg gcggtgcgcg gtaaactggt gccgcaggat
     3661 ccgaatgatg aaccggcgtc ggacctgctg aagcgtattg cggcggaaaa agcagagctg
     3721 gtgaagcaag gcaaaattaa aaagcaaaaa ccactgccag aaattagtga ggaagagaag
     3781 ccgtttgaat tgccggaggg atgggagtgg gtgcacctcc ctgatattta ttgctcaatt
     3841 tctgagtcat ctagaaaaat aaaatcctcc gaaatactac ctgagggaaa atatcctgtt
     3901 atcgagcaaa gccaagaatt tatttctggt tattgcaaca atgaatgtct gttaataaag
     3961 ttaaataatc cagttatagt ttttggtgat cacacaagga atattaaatt tattgatttt
     4021 gattttgttg tcggcgctga cggtgtgaaa attttatcac ctatattaat ttgtgagcgt
     4081 ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc gagacatttt
     4141 aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga gcgaatagtt
     4201 gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc cctgaccagt
     4261 ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac
     4321 accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc
     4381 accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa
     4441 cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag
     4501 gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact gccgccaatt
     4561 agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg tattggtaat
     4621 atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt atatcaagta
     4681 gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac agtgaaagaa
     4741 tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta ttctaaaatc
     4801 cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag tgctgatatg
     4861 tatccattag actgttacat taatccaaat tacttattga aatatatcct ttccattcca
     4921 tttcttatgc aagtcaaaaa agcagagaac agaataaaaa tgccgaaact taattctgac
     4981 agtttttaca atattattgt tgcgattccc ccctataatg aacaacaagc aatatttgat
     5041 aagataaatt ctattgaagc agtatgcaat ggtcttattt cttatattgg gatttatcat
     5101 aaaacccaac tgcatcttgc agacgcactc actgacgcag cgataaacta attccctcgc
     5161 cctcacaaaa ccaataaagc ggctaaatag ccgctttatt cacatcacca aaatttatat
     5221 ttcacgctaa ttttcatctt attttcatta atttttagct ggactttccc tgtatttact
     5281 gatgatatat ccaggtattt agcacggtgc ggatgtgcgc caacacactc gcaccgctaa
     5341 tcacaatcac tatttataga gaatagtaat catgactgac acgcattcta ttgcacaacc
     5401 gttagaagca gaagtctccc cggcaaataa tcgtcaatta accgtgagtt atgcgagtcg
     5461 ttacccggat tacagccgta ttcccgccat caccctgaaa ggtcagtggc tggaagccgc
     5521 cggttttgcc accggcacgg cggtagatgt caaagtgatg gaaggctgta ttgtcctcac
     5581 cgcccaaccc gccgccgccg aagagagcga actgatgcag tcactacgca aggtgtgtaa
     5641 gctgtcggcg cgtaaacaaa ggcaggtaca ggaatttatt ggggtaatta caggtaaaca
     5701 gaaagtcgcc tgatacattg acccggtagc gccagtgcta ccgggtatta ttcgccgcgt
     5761 tataccaacg cgctctccat ctgtttttca aatgcctgac gaattaatcc cataatgtaa
     5821 ggaagttgtg cgaggtcgct gaaaccaatt tctacatcgc cattacccca gcgcccaaca
     5881 tttgtcacat ctttggcaat acctttcgga tcgaccagtt catgaaactg catattaagc
     5941 gacaatcgca gtcggctttt ttgcggcaca acatcaacaa aattcgtttc cgctttaaac
     6001 gcaatatata gcttcaatac ttcctgcgtg atccctgcgt ccaggcgcat aacttcatcg
     6061 cgaagatg
//
LOCUS       DAJTYI010000185         6024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYI010000185 DAJTYI010000000
VERSION     DAJTYI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..88)
                     /locus_tag="OO564_003732"
     CDS             complement(<1..88)
                     /locus_tag="OO564_003732"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6654669.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO564_003733"
                     /gene_synonym="virG"
     CDS             complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO564_003733"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6654670.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4087..5289
                     /gene="virA"
                     /locus_tag="OO564_003734"
     CDS             4087..5289
                     /gene="virA"
                     /locus_tag="OO564_003734"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6654671.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5468..6024
                     /locus_tag="OO564_003735"
                     /pseudo
     CDS             5468..6024
                     /locus_tag="OO564_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    5749..5865
                     /locus_tag="OO564_003735"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1839 a         1367 c         1073 g         1745 t
ORIGIN      
        1 gtgcattagt ttctgcaata ccagtaatct gaggcgcata accacgaata tttggcggta
       61 gcattcgttc atcgctttct aatgcaattc ctgtgtaacg ccaagcaaca gcgcattctg
      121 gcgcatgatc tggtaaacac gtttggcatt gatcgcaggc ataccatcat gtgcacaaaa
      181 acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac acgccctgca
      241 tttttattat cagaaggtat atttcacacc caaaatacct tgggtatctc tgtaactgtt
      301 attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa gacgcccccc
      361 cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg acatgccatt
      421 cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa atggttcgaa
      481 cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac gcaatcccaa
      541 ttttgcttga atattattga ccccagagga tgtgaccata gtgccattgt gttcaatata
      601 gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat acaatgcgtt
      661 gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag cttccaaagc
      721 tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt tgactgtatt
      781 attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt ctttttcatc
      841 ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc gtgaactata
      901 actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca taatacccac
      961 atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa tctgataaac
     1021 catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat tatgtccacc
     1081 aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat caacaatagt
     1141 gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta tcaggttagc
     1201 aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca aattaagtga
     1261 agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt gattgctcat
     1321 ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg tattcagtcc
     1381 agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac ggcctttctg
     1441 aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt caagagtttt
     1501 atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg catggcctgc
     1561 aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca aagataatat
     1621 tccgcccttt ccatgccaat tacctttctg aacataggtc tgaccgacat ttgacgaact
     1681 attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga caatgacggg
     1741 gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat ttaataaagt
     1801 gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa caataacacc
     1861 agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat tgatgttggt
     1921 gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg attttgttag
     1981 ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca gaatagtact
     2041 aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc catctgtatc
     2101 attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt ttgtatagtc
     2161 aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac tggaacctgt
     2221 tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat ttataatagc
     2281 ttccactttt acattgttgc caaatgacat atttccagaa gcttccagac tactgattcc
     2341 agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag tgaatttgtt
     2401 acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa tagttggtgt
     2461 ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa cctgtacatc
     2521 accagtaatg acagaacctt catggagtat taatgaattg tttccaccag tgatgtggat
     2581 agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta tatatccgct
     2641 attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac cattaccgcc
     2701 taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat taccaccgta
     2761 gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg tgcccttatt
     2821 gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat caccctcacc
     2881 ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg gtagattgac
     2941 tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat cgccaccatg
     3001 tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa cagcctcacc
     3061 accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca tgccttgatt
     3121 gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac aactattacc
     3181 atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag agttaatccg
     3241 tagcttagtt ctagatgcat gagaggggtt ggaagaagtt atataccacg catccattcc
     3301 atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt tttcataatt
     3361 gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag ctattggccc
     3421 cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc atgttttttc
     3481 ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat tacaaaaaaa
     3541 tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc cctgaaaaga
     3601 ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac aaagcctcaa
     3661 ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc tgtgtcttgc
     3721 tttgaaaaaa agaggggaaa agctatatgt tcgataataa attaataatt ttactatgat
     3781 agtctgcttc agaccgcaac ttttcagtcc tttctgtacg cttgcccaaa gtttaaccac
     3841 tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc ccaaagccaa
     3901 taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca ataactgcat
     3961 aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt ccattttatt
     4021 atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata catcaggaga
     4081 aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc atggatgtca
     4141 actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga cgttttactg
     4201 aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa atatagcgaa
     4261 aaaaaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga cggagagttt
     4321 gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg catatgttcg
     4381 gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa agaatataat
     4441 aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca tactattaca
     4501 gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat tgaaacagcc
     4561 attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa agacactgat
     4621 tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag caatagtaca
     4681 aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct aattaatgag
     4741 ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa
     4801 gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca aacagaaaaa
     4861 cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac atctgcattc
     4921 tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca aattatgtcc
     4981 gaaaacatag aaaacaggct taatttcatg cctgaacaac gagttattaa caattgtgga
     5041 catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc tgcctctggg
     5101 gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg taatggtgtt
     5161 ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc aagcagtgaa
     5221 gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat catatctcct
     5281 gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct tcagaacagc
     5341 tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc agcccatcct
     5401 ttttatatca ttatgaaccg ccccggaaat cctggagact aaacttcctg agaaagaggt
     5461 aaacaggatg actaaaaata ctcgtttttc ccccgaagtc cgtcaacggg cagtccgtat
     5521 ggttctggaa agtcagggcg aatatgactc acaatgggcg acaatttgtt ccattgctcc
     5581 aaagattggc tgtacgccgg agactctgcg tgtctgggtt cgccagcatg agcgggatac
     5641 cgggggcggt gatggagggc tcaccaccgc tgaacgtcag cgtctgaaag agctggagcg
     5701 tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc caggcttccg cttattttgc
     5761 gaaggcggag ttcgaccgcc tctggaaaaa atgatgccac tgctggataa gctgcgtgag
     5821 cagtacgggg tcggaccgct atgcagcgaa ctgcatattg ccccgtcaac gtattaccac
     5881 tgtcagcaac agcgacatca tccggataaa cgcagtgccc gtgcgcagcg cgatgactgg
     5941 ctgaagaaag agatacagcg cgtatacgat gaaaatcaca aggtatacgg tgtgcgtaaa
     6001 gtctggcgtc agttgttacg ggaa
//
LOCUS       DAJTYI010000186         6003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTYI010000186 DAJTYI010000000
VERSION     DAJTYI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..93)
                     /gene="yjbD"
                     /locus_tag="OO564_003736"
     CDS             complement(<1..93)
                     /gene="yjbD"
                     /locus_tag="OO564_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6654672.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(346..1695)
                     /gene="lysC"
                     /locus_tag="OO564_003737"
     CDS             complement(346..1695)
                     /gene="lysC"
                     /locus_tag="OO564_003737"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6654673.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2220..3869
                     /gene="pgi"
                     /locus_tag="OO564_003738"
     CDS             2220..3869
                     /gene="pgi"
                     /locus_tag="OO564_003738"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6654674.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4331..4573
                     /locus_tag="OO564_003739"
     CDS             4331..4573
                     /locus_tag="OO564_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654675.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4687..5325
                     /locus_tag="OO564_003740"
     CDS             4687..5325
                     /locus_tag="OO564_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6654676.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5322..>6003
                     /locus_tag="OO564_003741"
     CDS             5322..>6003
                     /locus_tag="OO564_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6654677.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSET"
BASE COUNT         1501 a         1566 c         1542 g         1394 t
ORIGIN      
        1 accatgcgca atccgcgccc gatccagcag tgttttaagt tcacgctgct cgcgttcagt
       61 catctctttt tgggtaatac gggggagtgc catgatggtg ccctcagtga gccagagagt
      121 tcagtgtacg ggaattcgtt tgcgagcaga actgataata ttcattttgc cttctcttaa
      181 ctatttatct ctttcgataa ttaattagag atgcagataa aaaaattaaa ggcaattatt
      241 ctccgataaa gacgttggtt aacagagtac aggctcgttt attgatgagc atagtgacaa
      301 gaaaatcaat acggcccgaa atatagcttc caggccatac agtatttact caaacaaatt
      361 actatgcagt ttttgcacca cctgctcggc atcttcgccg ggcaccagga agcacaggtt
      421 atggctggat gcgccgtaac aaatcatgcg aatgttgaac ggttccagta cgccgaatac
      481 ctctttgcca acgccgcagg cttttgacag gtcattgcca atcaacgcga ccagcgccag
      541 accttcttcc acctccaccc ggcacagtgc ggaaagctcc atcagcagag attgcgtcag
      601 caacgtatcg ccagtggagg ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc
      661 tgacgtggtg attaagtcta ccgaaatatt atgccgcgcg aggatgccga aaacttccgc
      721 gaggaagccg cgagaatgca gcatattcag gctgtgcaaa gtgagcagag tctgattgcg
      781 acgaagcgcc agcgcgcgga acagcggcgg attttcagtt ttattgcaca ccagcgtacc
      841 acctgcgcgt gggtctttgc tggagccgac aaagaccggg atatcgctgc gtactgcggg
      901 tagcaacgtt gccggatgca gtacttttgc accaaaagtt gccatctctg ccgcttctgc
      961 aaacgcgatt tcatcaatgc gttttgctgc ggaaactacg cgtggatcgg tggtgtagat
     1021 gcccgggacg tcggtccaga tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc
     1081 tgccgtataa tcgctgcctc cacggccaag cgtcgttgta cgacctttat tttcgctacc
     1141 gataaatccc tgggtgatca ctaagccttc attgaggcgt gggagcagct gcagcgcggc
     1201 cagttccgcc agcgcggcta catctggctc tgcacgacca aatcggtcgt tggtacgcat
     1261 cactttacgt acatcaaacc actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa
     1321 cagcagggtc gacatcagct cgccgtggct gaccagctca tctgtcagcg ccggagacgt
     1381 tgccagcgcc gccgcttctg ccagaacagt aatgttctcc agcagacgtt caatctcttc
     1441 acggataacg ttcgggtaac gcagacgttc cagaatggca aactggatgt tgcgaatagc
     1501 gtcgagtttt tcgaatcgct cgccaggttc cagcccttcc gctaaagcga ccagcagatt
     1561 agtgatacca gcagaagccg agaggactac taaacgcacg ttggcatcag aaagcacaat
     1621 atcagcgctg cggttcatgg cgtcaaaatc agctacgctg gtaccgccaa atttggagac
     1681 aacaatttca gacataacta cctcatgtca ggggatccat tttcagcctt ggcacaaggg
     1741 aagagcggaa gacgggtggg cgcagagcga tacttcgcta ctattttcac ccagaagtgc
     1801 tccaccactt gcgaaacgcc cgactgcgaa cgcttctggt gacaacccag gggattcagc
     1861 ccctgtagcc gatgatgaac gtggccagcc gttcaatcac ctcggcgatg caccccctca
     1921 ggtgttatca caggactggc tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc
     1981 ctgcgctagc gcaggtagta catttataaa taaagggtga gcggggcggt tgtcaacgac
     2041 ggggtcatgc ggatttttca tccactcctg gcggtcagta gttcagctaa taaatgcttc
     2101 actgcgctaa gggtttacac tcaacattac actaacggca ctaaaaccat cacatttttc
     2161 tgtgactggc gctacaatct tccaaagtca caattctcaa aatcagaaga gtattgctaa
     2221 tgaaaaacat caatccaacg cagaccgctg cctggcaggc actacagaaa cacttcgatg
     2281 aaatgaaaga cgttacgatc gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt
     2341 tctccgcaac cttcgacgat cagatgctgg tggattactc caaaaaccgc atcactgaag
     2401 agacgctggc gaaattacag gatctggcga aagagtgcga tctggcgggc gcgattaagt
     2461 cgatgttctc tggcgagaag atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc
     2521 tgcgtaatcg tagcaatacc ccgattctgg ttgatggcaa agatgttatg ccagaagtca
     2581 acgcggtgct ggagaagatg aaaaccttct cggaagcgat tatctccggt gagtggaagg
     2641 gttataccgg caaagcaatc actgacgtag tgaacatcgg gatcggcggt tctgacctcg
     2701 gcccgtacat ggtgaccgaa gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg
     2761 tttctaacgt cgatgggact cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca
     2821 cgctgttctt ggtagcatct aaaaccttca ccactcagga aaccatgacc aacgcccata
     2881 gcgcgcgtga ctggttcctg aaagcggcag gtgatgaaaa acacgttgcg aaacattttg
     2941 cggcgctctc aaccaatgcc aaagccgttg gcgagtttgg tattgatact gccaacatgt
     3001 tcgagttctg ggactgggtc ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga
     3061 ttgttctctc catcggcttt gataacttcg ttgaactgct ttctggcgca cacgcaatgg
     3121 acaagcattt ctccaccacg cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg
     3181 gcatctggta caacaatttc tttggtgcgg aaactgaagc gattctgccg tatgaccagt
     3241 atatgcaccg tttcgcggcg tacttccagc agggcaatat ggagtccaac ggtaagtatg
     3301 ttgaccgtaa cggtaacgtt gtggattacc agactggccc gattatctgg ggtgaaccag
     3361 gcactaacgg tcagcacgcg ttctaccagc tgatccacca gggaaccaaa atggtaccgt
     3421 gcgatttcat cgccccggct atcacccata acccgctctc tgatcaccac cagaaactgc
     3481 tgtctaactt cttcgcccag accgaagcgc tggcgtttgg taaatcccgc gaagtggttg
     3541 agcaggaata tcgcgatcag ggtaaagatc cggcaacgct tgactacgtg gtgccgttca
     3601 aagtgttcga aggtaaccgc ccgaccaact ccatcctgct gcgcgaaatc actccgttca
     3661 gcctgggtgc gttgattgcg ctgtatgagc acaaaatctt tactcagggc gtgatcctga
     3721 acatcttcac cttcgaccag tggggcgtgg agctgggtaa acaactggca aaccgtattc
     3781 tgccagagct gaaagatgat aaagaaatca gcagccacga tagctcgacc aatggtctga
     3841 ttaaccgcta taaagcgtgg cgcggttaat catcatgcgt gcctgatgcc gatatgtagg
     3901 ccggataagg cgtttacgcc gcatccggca accggagcct gatgccaata tcgacattaa
     3961 tgcatagtat gaggagttaa ataaccttaa aaacaatgcc gacaataagt cggcattgtt
     4021 ttatcagata aatccccttg tctgtgattt aacggaaatc ataccgtgag gttaatccta
     4081 aaatagattt ttaatcgttg tttatttcgg agaatacgca gattaattac ttttgttttt
     4141 attttaagtt tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt
     4201 cattccaatt atcctaaaac tccatcgcta attccccgcg ccgtaattcg catgctttag
     4261 ttgtgtatac tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag
     4321 ggaaattgtt atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac
     4381 ttctgcgtgg gctgcacctg tacaggtggg cgaagcggca gagtcggcag caacgtcggt
     4441 ttcggcgggg agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc
     4501 gctcgcggta accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac
     4561 cagtacccag taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt
     4621 gaagtatgaa gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga
     4681 agagttgtga agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc
     4741 acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag
     4801 ctgacggacg atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc
     4861 gggccgcaat tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact
     4921 caggatcaag ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc
     4981 gacaacctga ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt
     5041 gttgatggtg caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac
     5101 gccaccgcac gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat
     5161 gaaaccctgg ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac
     5221 cgctactgga tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat
     5281 tatttcccgg tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg
     5341 tcgcgttgct tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt
     5401 tcagcaatgg cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg
     5461 tagggcaacc tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg
     5521 caacggcggc agcattgcgt cagcagcagg cgttgctgac acggctggca gagcagggcg
     5581 cagattccag caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat
     5641 tgaaagtgac aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac
     5701 gcggtaaccc gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg
     5761 tcacgttgtt cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg
     5821 tggcgagcta tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg
     5881 tcgtttaccc ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg
     5941 tagagccgat gcccggcagc attatttatg ttggcctcgc agattccgtc tggagtgaga
     6001 ctc
//
LOCUS       DAJTYI010000187         5766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYI010000187 DAJTYI010000000
VERSION     DAJTYI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..535
                     /locus_tag="OO564_003742"
     CDS             <1..535
                     /locus_tag="OO564_003742"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6654678.1"
                     /translation="LITPTGIPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQ
                     SRPDANAVVHNHAVHCTAVSILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHV
                     ALALKNRKATLLQHHGLIACEVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEE
                     IAVVLEKFKTYGLRIEE"
     gene            563..1711
                     /gene="fucO"
                     /locus_tag="OO564_003743"
     CDS             563..1711
                     /gene="fucO"
                     /locus_tag="OO564_003743"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6654679.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(1764..2519)
                     /gene="xni"
                     /locus_tag="OO564_003744"
     CDS             complement(1764..2519)
                     /gene="xni"
                     /locus_tag="OO564_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6654680.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2630..3997)
                     /gene="sdaB"
                     /locus_tag="OO564_003745"
     CDS             complement(2630..3997)
                     /gene="sdaB"
                     /locus_tag="OO564_003745"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6654681.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4055..5344)
                     /gene="sdaC"
                     /locus_tag="OO564_003746"
     CDS             complement(4055..5344)
                     /gene="sdaC"
                     /locus_tag="OO564_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6654682.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1435 a         1445 c         1541 g         1345 t
ORIGIN      
        1 gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata ttgtctttat
       61 tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc gtttccatat
      121 ggcagcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg ccgttcattg
      181 cacggcagtt tccattctta accgatcgat ccccgctatt cactacatga ttgcggcggc
      241 tggcggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg aactttctga
      301 acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc atgggcttat
      361 cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg aagtgctggc
      421 gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga gcgatgaaga
      481 gattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag agtaatttcg
      541 taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa cggcatggtt
      601 tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt atcagaaggc
      661 gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag tgaccgataa
      721 gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca acccaacaat
      781 tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt acctgatcgc
      841 tattggtggt ggttctccac aggatacttg taaagcaatt ggcattatca gcaacaaccc
      901 ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac ccagtgtacc
      961 gattctggca atccccacca cagcaggtac tgcggcagaa gtgaccatta actacgtgat
     1021 cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata tcccgcaggt
     1081 ggcgtttatt gatgctgaca tgatggatgg tatgcctccg gcgctgaaag ccgcgacggg
     1141 tgtcgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt gggcactaac
     1201 cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag gatcggttgc
     1261 tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg gtatgggctt
     1321 ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg cgttttataa
     1381 cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct ataacgctga
     1441 ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag tggaaggtat
     1501 gagcctggaa ggggcgcgta atgccgctgt tgaagcggtg tttgccctca accgtgatgt
     1561 cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattt cggcactggc
     1621 tcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa cgcttgagga
     1681 tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg ccggatacga
     1741 tttgcggcgt atccggctcg ccgttaccgt accaaccgca attgctgtaa attgccgtcg
     1801 atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat ctctttatgg
     1861 gtttctaatt ttttgcgcca cttttcggca accgcatcca gattctcata tatcccttcc
     1921 agactctgaa actcgaccag cagctgcgtg gcgctttttg gtccgattcc cgcaacaccc
     1981 ggtacctttg aactgctgat ccccgcaagt ccccagtaat cgggcaactg ctgcggttga
     2041 acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa gtaatcacga
     2101 atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac aatcgttgcc
     2161 tgatgcccgg ccagtgtcac tttgaccgcc agtgtggcgg ctaagtcatc ggcttcgttg
     2221 ccgctggctg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa tgcaggcatc
     2281 tcgtcgtgca actcttccgg cattggcggt cgatccgctt tgtaatctgg taaacgctga
     2341 tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg ggttggctgg
     2401 ctgtgcataa tgagctgatc gagcgcatgc tggcaggttt cgacacaggg cgaaccctga
     2461 acggcatgaa tgcgacggat aagattcagt gcatcgacaa taagcaaatg aacagccacg
     2521 ataaccccct taaagatcag gcgataaggg taacattcga tgccggaaca ggctatggct
     2581 ggttgagaaa acaggaagag gcttcgcaaa acgaggcctc tgggagagat taatcgcagg
     2641 caacgatctt catcgccagg ccgccgcgag aggtttcgcg gtacttggcg ttcatgtctt
     2701 tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggt tcgctggtac
     2761 ggcgcagtgc catacgcgcg gcgttcaccg ctttcactgc tgcaatggcg ttacgctcaa
     2821 tgcacggtac ctgaacctgc cccgcgaccg ggtcacaagt cagaccgagg ttgtgctcca
     2881 tcgcgatttc cgctgcgatg caaacctgcg ccgggcttgc gcctaacagt tctgccagac
     2941 cagctgccgc catagagcac gccacgccaa cttcaccctg gcaacccact tcggcaccag
     3001 aaatcgacgc gttcatctta taaagagaac caatggcgct ggctaccagc aggtaacgag
     3061 ccagtgagtt agcgttcact tcgcggataa acttatcgta gtacgccagc acagccggga
     3121 taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg accgccagca gcgttctctt
     3181 cgttcactgc cagtgcaaac atgttgatcc agtcaacaac cgccatcggg tcagtggtgg
     3241 ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga acgcgcagtt
     3301 tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc atgacttccc
     3361 agacgttcgc caggtgctgt tccagctctt ctttactgtg cagcgccagc tcgtttttca
     3421 tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga tcggctgctg
     3481 aactgtacgg ataaggaact tcaaccggtg cgctatcctg ctggccaaaa tgctcttcat
     3541 caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact ttatcgcccg
     3601 ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca tggaagttca
     3661 tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc aggcgaccat
     3721 gagtattcac atcctgaata aaaccgggga tggaatcgat atccacggta tccggcaggt
     3781 tacccgccag gcccataata atggagatat ctgtgtggtg gcctttacca gtcagagaga
     3841 gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta cgggcaatca
     3901 gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa ctggaagggc
     3961 caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg cgttaaaacg
     4021 gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag tagaagattg
     4081 cggagattgc aatcagcccc atcacgacaa cgaatacgtt gctgatgtga ccgctgtact
     4141 tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg atcatcgcga
     4201 tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg gtggcaacaa
     4261 tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg atttcgatag
     4321 acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca cgtgcgccca
     4381 ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga gccatccacg
     4441 cgataaccgg tgcgttaaag tggttagcca ggtaagacag aatcgagatg ttctgctctt
     4501 tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg aagaacatta
     4561 cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc tgttctgcca
     4621 tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga gagtggttga
     4681 acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg tttccggttg
     4741 cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc gggatcaggt
     4801 acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc atcgctttaa
     4861 cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc cacgatcagg atcagcgaca
     4921 gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt tcaacggtat
     4981 tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag tagagcaggg
     5041 taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg atgtcttcgc
     5101 ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa aacgtcatcg
     5161 ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg gcgttgattg
     5221 gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc cacatggtgt
     5281 ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt tgagtcgttt
     5341 ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc agcccgggaa
     5401 ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat ttaccgctgt
     5461 aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat cttgatccca
     5521 aatacaataa tctgcttgga agatagatta tcccaacgct ttactctgta aatcaaaagt
     5581 aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct tccggggcgc
     5641 gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg gcgataattt
     5701 ctttttcata gcgataaaat acggtttatg aaaataggga aacgtttgcg ttgcaacata
     5761 aattgt
//
LOCUS       DAJTYI010000188         5371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYI010000188 DAJTYI010000000
VERSION     DAJTYI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..238)
                     /locus_tag="OO564_003747"
     CDS             complement(<1..238)
                     /locus_tag="OO564_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6654683.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQS"
     gene            775..2676
                     /gene="mtlA"
                     /locus_tag="OO564_003748"
     CDS             775..2676
                     /gene="mtlA"
                     /locus_tag="OO564_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6654684.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2771..3919
                     /gene="mtlD"
                     /locus_tag="OO564_003749"
     CDS             2771..3919
                     /gene="mtlD"
                     /locus_tag="OO564_003749"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6654685.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3919..4506
                     /gene="mtlR"
                     /locus_tag="OO564_003750"
     CDS             3919..4506
                     /gene="mtlR"
                     /locus_tag="OO564_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6654686.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4518..4727)
                     /locus_tag="OO564_003751"
     CDS             complement(4518..4727)
                     /locus_tag="OO564_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654687.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5011..>5371
                     /locus_tag="OO564_003752"
     CDS             5011..>5371
                     /locus_tag="OO564_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6654688.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1368 a         1325 c         1399 g         1279 t
ORIGIN      
        1 gtgactgcat accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg
       61 gccagataac atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat
      121 gggggtgatt gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat
      181 aaaaaatcac cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat
      241 ctgcctcgga ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg
      301 ctgatatttt gacaataatc cgggttcgcg attctcacca taacaccaaa gaataatttt
      361 tagaggtgat gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta
      421 gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac
      481 aaaccatcaa aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc
      541 accgccttgt ttctttttta cacaggcgtt ttgtgatgag cgtcacgtca attacctctc
      601 taccccctat atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg
      661 attcagtgcc agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca
      721 aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca
      781 tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca
      841 aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg
      901 ttaccgaacg agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg
      961 ctgatcggtt ataccggtgg taagctggta ggcggcaaac gtggcggcgt agtcggtgcc
     1021 attaccacca tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt
     1081 gcaggtccgc tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc
     1141 aaatccggtt ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc
     1201 gctattctgg cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct
     1261 gcgggcgtta acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa
     1321 ccggcgaaaa tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt
     1381 attcagcagt cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt
     1441 ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct
     1501 gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat
     1561 gtgctgatga atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc
     1621 acgctgacta tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct
     1681 gtactggcga tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg
     1741 atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa
     1801 gatgatattg aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca
     1861 tctccgctat ctgcgggcaa tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt
     1921 gcctgtgacg ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt
     1981 caggatgcag gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca
     2041 gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg
     2101 caggcacagc atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc
     2161 gaacgtctcg ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagcttt
     2221 gacgattcca gtgctaacct gttcaaactg ggcgcggaga acatcttcct cggtcgcaaa
     2281 gcagcaacca aagaagaagc gattcgtttt gctggcgagc agctggtgaa aggcggttac
     2341 gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa aactgacccc gacttatctg
     2401 ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt actgaaaacg
     2461 ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt
     2521 gcccgtctgg tgattggtat tgcagcccgt aacaacgagc acattcaggt tatcaccagc
     2581 ctgaccaatg cactggatga tgagtccgtc atcgagcgtc tggcacacac caccagcgtg
     2641 gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca atcctaccct ctcctcacgg
     2701 agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa
     2761 ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg
     2821 taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact
     2881 tgatgccctg aatgcccgtc atagctatca ggttcatgtg gtcggtgaaa ccgagcaggt
     2941 agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat
     3001 tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc
     3061 ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat
     3121 catcgcctgt gaaaacatgg tacgcggcac cacgcagctg aaaggtcatg tgatgaacgc
     3181 cctgccggaa gacgccaaag cgtgggtaga agagcacgtt ggctttgtcg attccgccgt
     3241 tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga
     3301 aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc
     3361 aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa
     3421 cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga
     3481 cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc
     3541 ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat
     3601 cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc
     3661 gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata
     3721 cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga
     3781 agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc
     3841 gctggcacag atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc
     3901 ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga
     3961 ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag
     4021 cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc
     4081 gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat
     4141 gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa
     4201 ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg
     4261 gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc
     4321 cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa
     4381 ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca
     4441 acaatggtcc tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa
     4501 aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa
     4561 tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg
     4621 ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct
     4681 acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg
     4741 tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc ggtaaagatg
     4801 acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttc
     4861 tatagatttc ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt
     4921 gcagaactat tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc
     4981 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
     5041 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
     5101 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
     5161 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
     5221 ccgttccagc gagcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
     5281 gttcgcggac tggtggttgt gcacccaatg accgcgctgg ggcgcgaaat gggcctggaa
     5341 gagatgactg gattttctaa gactacgttt t
//
LOCUS       DAJTYI010000189         5371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYI010000189 DAJTYI010000000
VERSION     DAJTYI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(296..580)
                     /locus_tag="OO564_003753"
     CDS             complement(296..580)
                     /locus_tag="OO564_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6654689.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="OO564_003754"
                     /pseudo
     CDS             614..1420
                     /locus_tag="OO564_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="OO564_003755"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="OO564_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="OO564_003756"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="OO564_003756"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6654690.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="OO564_003757"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="OO564_003757"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6654691.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="OO564_003758"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="OO564_003758"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6654692.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..>5371)
                     /locus_tag="OO564_003759"
     CDS             complement(4472..>5371)
                     /locus_tag="OO564_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6654693.1"
                     /translation="QQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPWYTHLG
                     IKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKTTLPDS
                     ASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRILLIMGE
                     WLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCI
                     MLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICNAIEID
                     LLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1350 a         1283 c         1440 g         1298 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt g
//
LOCUS       DAJTYI010000190         5366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYI010000190 DAJTYI010000000
VERSION     DAJTYI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1028
                     /locus_tag="OO564_003760"
     CDS             <1..1028
                     /locus_tag="OO564_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6654694.1"
                     /translation="VGIMGTSLSGLDAAMAVAIQHGSFIEDDKQHVVFHRDNASEKLN
                     ITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKGEEGLLDRVFRLIVEEIKFA
                     DPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNLQEVERNKREKHTVPWRYVI
                     LRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPSESIRRLLALREAGIIHILA
                     LGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKVKDIPFPGLREQLQKTGDEI
                     PDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTACAEIGEAMARAVVKPASRA
                     RRRLSFD"
     gene            complement(1040..1852)
                     /locus_tag="OO564_003761"
     CDS             complement(1040..1852)
                     /locus_tag="OO564_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654695.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1856..2641)
                     /locus_tag="OO564_003762"
                     /pseudo
     CDS             complement(1856..2641)
                     /locus_tag="OO564_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2638..3306)
                     /locus_tag="OO564_003763"
     CDS             complement(2638..3306)
                     /locus_tag="OO564_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6654696.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3370..4008)
                     /locus_tag="OO564_003764"
                     /pseudo
     CDS             complement(3370..4008)
                     /locus_tag="OO564_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4021..>5366)
                     /locus_tag="OO564_003765"
     CDS             complement(4021..>5366)
                     /locus_tag="OO564_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6654697.1"
                     /translation="TARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTYKSVFDLGPAA
                     EKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTIFPNGPKDFED
                     KDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKRVLPAEEYAEIRWDQL
                     EAGDVNFIKDFYYRLAHRVGELSHLAYGSYAIAERWNLGKEYWGYAKNKLWSPFGYPV
                     HHANEASAQVGFIVNCMFNRDCMTHTHINFIGSGLPLKLQREVAKELFGSEDAYDETK
                     NYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKA
                     ITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRNEHDLICSWVFDKDPQIPVFTE
                     GTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         1374 a         1435 c         1243 g         1314 t
ORIGIN      
        1 acgtgggtat tatgggaaca tccttgagcg gactggatgc ggcaatggca gtggctattc
       61 agcatggttc gttcattgaa gatgataaac aacacgtcgt ttttcaccgc gataacgcaa
      121 gtgaaaagct aaatattacg ttgatgtcgc gcacgggtat tttacccgaa gccgatttct
      181 attgccctat tccctgcgag cccttacaca tcgtcaccga tcaggcatta aatgctgaga
      241 ttcaaaaagg tgaagagggc cttttggatc gggtatttag attgatagta gaggaaatca
      301 agtttgctga tccagactgg agccaacgca tagccttaga gagcctgaat gtcgattcct
      361 ttgctcaagc ctggtttgcc gagcgcaaac aacgcgaccc atttgactgg gcagaaaaaa
      421 atctccagga agtcgaacgc aataaacgag aaaaacatac tgttccctgg cgttatgtca
      481 ttctgcgcct gcatgaagcc gtacaggaaa ttgttcctca tctgaatgaa cacgaccata
      541 aacggttcag taaaggcctt gccagggttt tcatcgataa ttatgcggca atcccttcag
      601 agtctattcg tcgactactg gccttacgtg aagcggggat cattcatatt ctcgccctcg
      661 gtgaagacta cgaaatggaa attaatgagt cgcgcaccgt cctgaaaacg gaagacaaca
      721 gctactcgtt tgacgttttt attgatgccc gcggacagcg tccgcttaaa gtgaaagata
      781 ttcctttccc tgggctacgc gagcaattac agaaaacagg ggatgaaatc cctgatgttg
      841 gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg ttcggcgcgt
      901 tgccctggct gatgcacgac cagcctttcg ttcagggact tacggcatgt gcagaaattg
      961 gtgaggcgat ggctcgggcg gtcgtaaagc ctgcatcccg tgctcgtcgg cgtctttcgt
     1021 ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta agacgatagc
     1081 ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga actctttcgc
     1141 cgtcatgata tgccagtgcg gcgcttcccc ctagcccgag aataattgag tcgaccacgc
     1201 ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg tcgccgctgt
     1261 tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca ttactggcga
     1321 catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc gcaatccgac
     1381 tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc cgtgcgcgtt
     1441 caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg ctataaccca
     1501 tactggcaat ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc tcacaacagc
     1561 cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc tctccggggt
     1621 ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca tccgccagcc
     1681 attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct tcctcaatca
     1741 gtgcatgacg aatatcggca taactaccgc cgcgtgggaa atggcgcagg aaccagcgat
     1801 acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc attcgttagt
     1861 gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc cccgtggtgc
     1921 aaagataaag atgaccgaag ataaaaaaga ggcttataaa tgccagagca aaatgcgcct
     1981 gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga tagagacaca
     2041 acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca taggcgacct
     2101 gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct tccccctgca
     2161 taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac ccctgacgac
     2221 gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag ccgagccagc
     2281 atgccagtaa caaaaatcag caaacttcat gcaccgcaac cagactttta accgcagttg
     2341 cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca aagagtaacg
     2401 cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc tcaccatgat
     2461 cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc agcaccaacc
     2521 acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat tcgggagtga
     2581 actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc gacgggttca
     2641 tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg gttttccggc
     2701 accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa tttctgggct
     2761 atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa tgggcggaaa
     2821 gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt tagtgacagg
     2881 gttaaggtag cgaacctggt acggacaagc gccaatgcaa taactgcagc cgatgcactg
     2941 cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg tcgggcatac
     3001 gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt gatactgcgt
     3061 ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc cctgagcggg
     3121 gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc accgtgactc
     3181 gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta acagagaaga
     3241 cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc gtcgagtgaa
     3301 ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc atgacatctc
     3361 cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata agccataagc
     3421 cttgccatag gtcataaatc gctcgctaaa caagtacaac accgccgtcg atgactccac
     3481 ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcggtcgcgg cttcaatctg
     3541 cgcttcctag aagtaatatg gcgcttgcat cagcatggcg actttgtaag gacgaggata
     3601 cgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct gctccacaac
     3661 ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat agtaatcatc
     3721 ctgacttccc gtcatacaag cgatatccgc gtagtctccg ttctgcttaa tgctttccag
     3781 caaaactgac agttcctctt ctgcaaccga atagggtggc gagagaaaca cggtgcgcgc
     3841 caccagctgc ccagccgcag aatgctggcg aatgaactca gccaggattt cagcaggttc
     3901 tggttcggtt accgtatcaa cggggtgacg catcccctgc aaccattgcc gcttcgcctc
     3961 gtcaacttcg ctgactttcg ctaacggcaa ttcatcctga tggttcatct tacccattcc
     4021 ttacgcaggc agtagattgt gtgctgccag atcggcggca atatcttcca gccccagacg
     4081 ctgcaatgtt tcgcgggttg gacaaccaag ctgtgggtcc cagcccattt ctttgtagaa
     4141 catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt cagtaaagac
     4201 cgggatctgc ggatccttgt cgaaaaccca ggaacagata agatcgtgtt cattacgcat
     4261 atctttggtt tgcatcggtt ttaccgtgta ggcacgatgc aacgtaaaga tacgctctgc
     4321 agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga agaatttggc
     4381 ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg tcattggcca
     4441 gacccagttg cacagggtga cggcgttatg caaacagacc cgcaacagcg accactttgc
     4501 atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt aagcatcttc
     4561 agaaccaaaa agttctttcg ccacttcacg ttgcagtttc aatggcaagc cggaaccaat
     4621 aaagttgata tgggtatgcg tcatgcagtc acggttgaac atacagttaa caatgaaacc
     4681 gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg accagagttt
     4741 atttttcgcg tagccccagt actctttacc caaattccag cgttctgcga tggcatatga
     4801 accataagcc aggtgactca gctcacccac acgatgcgcc agacggtagt aaaaatcttt
     4861 aatgaagtta acgtcacccg cttccagttg atcccagcga atttctgcat actcttcagc
     4921 tggcagaaca cgcttgaaca cacctttgct gtaacaataa gtaaagtcgc gatgcaactg
     4981 cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca ggttaccaat
     5041 cacacgacca tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga tggtggtatg
     5101 gacaaagttt gctacacaag tgttaccacc tgtgctgggc acgccaaact ctttcacccg
     5161 aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccgc tcatttttac
     5221 tgtgtatttc tctgccgccg gtcctaagtc aaaaacggat ttataggtac gaaagccgac
     5281 cgtattttga ttgcctggcg gaatttcacc cgtttcaatc ggaccacctt cagccgcgcc
     5341 ccaaaacagc cctttacgtg ctgtcc
//
LOCUS       DAJTYI010000191         5352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYI010000191 DAJTYI010000000
VERSION     DAJTYI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..1169
                     /locus_tag="OO564_003766"
                     /pseudo
     CDS             1..1169
                     /locus_tag="OO564_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1205..1408)
                     /gene="ydfZ"
                     /locus_tag="OO564_003767"
     CDS             complement(1205..1408)
                     /gene="ydfZ"
                     /locus_tag="OO564_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6654698.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1586..2272)
                     /gene="ydfH"
                     /locus_tag="OO564_003768"
     CDS             complement(1586..2272)
                     /gene="ydfH"
                     /locus_tag="OO564_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6654699.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2361..3107)
                     /gene="ydfG"
                     /locus_tag="OO564_003769"
     CDS             complement(2361..3107)
                     /gene="ydfG"
                     /locus_tag="OO564_003769"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6654700.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3244..5289
                     /gene="dcp"
                     /locus_tag="OO564_003770"
     CDS             3244..5289
                     /gene="dcp"
                     /locus_tag="OO564_003770"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6654701.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1327 a         1287 c         1396 g         1342 t
ORIGIN      
        1 cagatggatg gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc
       61 tctctgacaa tcaccgaaaa agggtatttc cactctccgg cgaccggaca gttaatgctc
      121 gatcacccga tggtcgctgc cgacgtgcaa aatccccacc agccgaaaac agcaacatgg
      181 gtgattgttg aggcgctggc tcgccgtaaa gcggcaggac ttcccgcatt cccgcattta
      241 ccgtcatgtc atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt
      301 cctacgcgca agccgttgat gtaaaactgg cacaatggat cgaagataac gtgactttcc
      361 catcaacaat ggtggaccgt attgtgcccg cagtggcaga ggatacgctg gcgaaaatcg
      421 aacaacttac cggtgtgcgc gatcctgctg gcgttgcctg tgaacctttc cgccagtggg
      481 ttatcgaaga taactttgta gccggacgtc cggaatggga aaaagcggga gccgaactgg
      541 ttagtgatgt gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat
      601 tcctggcata tctgggttat cttgctggat atcagcacat taatgactgt atggaagatg
      661 aacattatcg tcatgcggcg tatggcatga tgttgcagga acaagcgccg acgctgaaag
      721 tgcagggcgt tgatttgcaa gattacgcta accgattaat tgcacgctat agcaacccgg
      781 cgttacgtca tcgaacctgg cagattgcga tggatggcag ccagaaattg ccacagcgga
      841 tgttggattc tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg
      901 gcgtcgcggg ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa
      961 tcagtgaccc actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag
     1021 cgcgcgtcca gtcattgctg gcgattaagg caatctttgg tgatgatttg ccagacaata
     1081 gcttgtttac tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggcgcgaaag
     1141 cgaccgtggc gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac
     1201 acacttagtt catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt
     1261 caacaactac ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg
     1321 acaggatctt gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg
     1381 cgttacggtt acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa
     1441 gcccacgtaa cgggcgcaat ataaatactc ctgtcgagtg aaatattttt tgtttttgat
     1501 caagctcaca tcttttttga tgcatgcatg agccgtaagg cggggggaaa agcctctcat
     1561 cccggatggg atgagagtaa atgaattact cttcgctaaa ccagttgctg ttttcctggc
     1621 ggatctggcg cacggattcg ctgatttcct gcaaatgttg cgtcattgca cgttctaccg
     1681 catcgccatc acgtttttgc agggcagaga aaatatcaag atgctggcgt aacagcattt
     1741 ctggtggaga aacatggtcg aaactcatat agcgcacgcg atcaacggtc gctttcaggt
     1801 tctcaatggt atcccacgcc agttgacagt cggcaatctg cgtcaggagt tgatggaagt
     1861 tgtcatcaag ttcaaaaaaa tcatccagtt gcttgcgctc aatggcaatg cgttgctggt
     1921 gaagattttg ttccagttgg tagcactggc tttcggtaat catgctcgcc gcccgacgcg
     1981 ccaccgcgca ctcaatggcc tgacggataa aactgccgtt gcgcacctgg gccatggaaa
     2041 ttttgttgac gtagctgcca cgctgcggac gaatttgaat caggccgttt tccgccagtt
     2101 taataaaggc ttcacgaacc ggctggcgtg acacattgaa acgaacagaa acttcttttt
     2161 ccgacaacgg tgtgcctggt gcaatcaggc aatggacaat gtcgcgacga agaatacgat
     2221 aaatctgctg attgacaggc tgtgtgggat taagttgcgt ttcgacggtc attcgttctt
     2281 gcttgtgagt gagttaactg catgagtcta ccactttttt gtgacaagca ataacccggc
     2341 agttacgccg ggtataaaaa ttactgacgg tggacattca gtccggcata gctttgggta
     2401 accggcatca tttccagggt attgatattg acgtgagcag gcagcgttga cacccaccag
     2461 acggcttcgc tgacatcttc tggcgtcaat gcaacggtat tttgataggt tttttccgct
     2521 ttaccgtcat cgcctttaaa gcggacattg gaaaactcgg tgccacccac cagacccggt
     2581 tcgatgtcgg tgacgcgcac cgccgtacca tgcagatccg tacgcagatt caggctaaac
     2641 tgacgaacaa acgctttcgt cgcaccgtaa acgttaccac cggcatacgg ccagctgcct
     2701 gccgttgagc caatgttaat aatatgacca tgattacgtt caaccatacc cggtaagacg
     2761 gcgcgcgtca tatatactag gcctttgttg ttggtatcaa tcatcgtttc ccagtcttca
     2821 acgctggctt tatgcgcagg ctccatgccc aacgccaggc cggcattatt taccaggata
     2881 tcaatattgc tccactcggc agggagcgat gccagcatct cttcaatagc ggcgcggttg
     2941 cgaacgtcca gttgggcgat atacagatta tctcccagtt cgtcttttaa ctcctgcaac
     3001 cgctcctggc gacggccagt ggcgataact ttatgccctt gttgaataaa acgacgagta
     3061 atgcattcac caaaacctgc cgttgctcca gttactaaaa cgatcatctc actgttcctc
     3121 aacgcttttg tgtgtcaata acatagcacg tgatgcttaa gagcgggtaa tactggtttg
     3181 caagattgca ctccgttatc agtaagccta cgctatggaa cacctcgcgt tcaggagaag
     3241 aaaatgacaa caatgaatcc tttccttgtg caaagcacac tgccgtatct ggctcctcat
     3301 tttgatcaaa ttgccaatca tcactatcgc ccggcattcg atgagggaat gcaacaaaag
     3361 cgggcagaaa ttgctgccat cgcgcttaac ccgcaaacgc ctgatttcaa caatactatt
     3421 ctggcactgg aacaaagcgg agaattaatt acccgcgtta ccagcgtctt ttttgcgatg
     3481 actgcggcgc ataccaatga tgaattacag cgtcttgatg aacagttttc cgctgaactg
     3541 gcggaactgg ctaatgatat ctatctgaac ggtgaattat tcgcgcgggt agatgctgtc
     3601 tggcagcgcc gtgaatccct ggggcttgat agtgaatcca tccgcctggt ggaggttatt
     3661 catcaacgtt ttgtccttgc cggagccaaa cttgcacaag ctgataaagc aaaattaaaa
     3721 gtactgaata cagaagctgc gaccctgacc agccagttta accagcggtt acttgcagca
     3781 aataaatccg gcggtctggt tgtgaacgat atcgcgcagc tggcaggaat gagtgagcaa
     3841 gagattgcgc tggcggcaga ggcggctcgc gagaaaggtc tggataacaa atggctgatt
     3901 ccgctgctga ataccaccca acaaccggcg cttgccgaaa tgcgcgatcg tgcgacgcgt
     3961 gaaaaactgt ttattgcggg ctggacgcga gcggaaaaaa atgatgccaa tgatacccgc
     4021 gctatcattc aacgtctggt agagattcgc gcacagcagg cgaaactgct tggttttcct
     4081 cattatgccg catggaaaat cgccgatcag atggcaaaaa cgccagaagc agcactcaac
     4141 tttatgcggg aaattgttcc agcggcgcgt caacgtgcga gcgatgaatt agcctccata
     4201 caggcggtta tcgataagca gcagggcggg tttagcgcgc agccgtggga ctgggcattt
     4261 tatgcggaac aggtacggcg ggagaaattt gatcttgatg aggcgcagct caagccatat
     4321 tttgaattaa acacggtgtt aaatgaaggt gtattctgga ccgcgaatca gctcttcggt
     4381 attaagtttg tcgaacgttt tgatattccg gtctaccatc ctgacgttcg tgtgtgggaa
     4441 atttttgatc ataatggcgt gggactggcg ttattttacg gtgatttctt cgcccgtgat
     4501 tcaaaaagcg gcggtgcatg gatgggcaat tttgttgagc aatcaacgct taatgaaacg
     4561 catccggtaa tttataacgt ctgcaattat cagaaacccg ctgccggtga gcctgcgttg
     4621 ttactctggg atgatgtaat aaccttattc catgaatttg gtcatacgct gcacggcctt
     4681 tttgcccgcc agcgttatgc cacgctttcc ggcaccaaca cgccgcgtga ttttgtcgaa
     4741 tttccgtcgc aaatcaacga acactgggca acgcatccgc aggtattcgc tcgctacgcc
     4801 cggcattatc agagcggggc agcaatgcct gacgaactgc aacagaaaat gcgtaatgcc
     4861 agcctgttca acaaagggta tgagatgagc gaactgctta gcgccgcact tctcgatatg
     4921 cgctggcatt gcctggaaga aaacgaagca atgcaggatg tcgatgattt tgaattgcgg
     4981 gcgctggtgg cggaaaatat ggatcttcct gctataccgc cacgctatcg cagcagttat
     5041 ttcgcccata tttttggtgg cggatatgct gcaggttatt acgcttatct gtggacgcaa
     5101 atgttggccg atgatggtta ccagtggttt gttgagcagg gcggattaac gcgtgaaaat
     5161 gggcagcgtt ttcgcgaggc gatcctttcc agaggtaaca gcgaagatct ggaacgcctg
     5221 catcgacaat ggcgcggtaa ggctcctcag attatgccga tgctgcaaca tcgtggcttg
     5281 aacatataaa caccaccaat ggctggcctg gagatatcgc caggccagcc tggttatagc
     5341 agtaattgca gc
//
LOCUS       DAJTYI010000192         5348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYI010000192 DAJTYI010000000
VERSION     DAJTYI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            89..703
                     /gene="istB"
                     /locus_tag="OO564_003771"
                     /pseudo
     CDS             89..703
                     /gene="istB"
                     /locus_tag="OO564_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(872..4966)
                     /gene="sepA"
                     /locus_tag="OO564_003772"
     CDS             complement(872..4966)
                     /gene="sepA"
                     /locus_tag="OO564_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6654702.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1310 a         1354 c         1246 g         1438 t
ORIGIN      
        1 ccttccaacc gagttgacga actgctgcca tggaacgtag ttctcaccga taaataagcg
       61 tcaatacggc cttccgttga cgcttacaga actcctgtgg cgcgagagtg aaaaactgcg
      121 tcgttataaa aaggaggccc ggttgccagt tgccaaaacg ctaagcgaat acgacttcat
      181 tcagttgccg gaactgaatg gagctcagtt ccagcaactc tgtgaaacga cagactgggt
      241 tgatgctgga gaaaacgttc tgctgttcgg ggacagcggg ttggggaaaa gccatctggc
      301 ggcagcgatc gtggatggcg tagtaggcca gggctaccgg gcccggttca acagcgcagg
      361 agagctgttg caggaactac ataaagccag agctcagttg aaactgaatg agctgctact
      421 gaaactggat cgctaccggg tgatagtggt ggatgatctt ggctatgtca aacgcgacag
      481 cgccgaaacg ggtgtgctgt tcgagttaat agcgcatcgc tatgaacgtg ggagcctggt
      541 gataaccagt aaccatccgt tcagcatgtg gggcagcatc ttcgtggatg agactatggc
      601 ggtggcggca gcagaccgac tgatccatca cggatatatg ttcgaactga aaggggaaag
      661 ctacaggaaa aagacagcga aggcagtaac aagcgtgact tgatgtcgca ctgaaggggc
      721 ggccagtata gttggcgcca gtcggcaaaa ctagttgacg tctaatacct ggacggtcac
      781 ttctgagaaa aggcatttac acagaatctt aaacaggctc acggagttct ttcagaaaaa
      841 ggcctgccga atggcaggcc tatcccattc gtcagaaaac ataacggaag ttggcgttta
      901 tcgcattatc cacattatat ttcccgaacg ctgatttctc cagctccagt cccaaacgca
      961 tattatcctt aatttccgca ttcatcccta ccgtcatcag catcctgcta tctttttcac
     1021 cttcgaaacg tttctctccg gaagcatcct gcagaaccgt ttctccgttc gccagcaggt
     1081 cgaactgata acccagcccg gctcgagctg tgattttcca gtcgtctccg gagaaggctc
     1141 ttcccacgtc aacaccagta cggccaatca gtgggttata atccttgtct ttcatgctca
     1201 gagccattcc ccggtcttcc cagctaaaag cttttcctga tacagaaccg taaaccagct
     1261 ctatctgtgg ctccacccag gactctttcg tcaggtggta acgataacca acttcagcac
     1321 cggtatacca ggaatgagag ctgtagtctt ttgttcccag tgaggcaaaa ttcgccgtgt
     1381 gctgattatc atgatggaga tatttaccaa tcaggtcaaa ataagctccg gaattaaaga
     1441 gtgcagaggc atacagaccg ccacccacgg atttgttttt tccactgaat gcgtggctgc
     1501 ttgcattgct gtccgtatag gtcagcaatg cccccgtaaa taaatccaca ccgtccagct
     1561 catgctttct gtcgacacca atctgaacgt gagtgtagtt atcgctgtag tcaccatctg
     1621 ccgaaccggt accattcatt atgcgtgccc agacaccggc atccccctga gtatcccgca
     1681 ggtcacccat acgtttgttc aggttattga cctctgtaag gaaggtctta taacctgatg
     1741 ccataaagtc ttttgcggct ttcgaggctc cggcatcagc ggtagtctga tatcctgtca
     1801 gcacccattt tgtggcatca tccgtttttt ccgtgctgat tactggcgta acgttactga
     1861 aaccaatctg ctgcgtaccg gcagtgaacg ttttctcatc actccctttc ggtgcggtaa
     1921 ttaacgtcac gctcatttca ctggctggtt tgtttaaaaa gtcaaccagg acgctattat
     1981 tactgcctga cagtttgtct gtgacattca actggtcagc ccggctgttg ttggtatgca
     2041 tcaggaacgt ggtattatct gcagaaagct cttttacttg taaagtggca aagtgtttcc
     2101 cgtcccccag ttcaattcgc gagcccttag ccttcagtgc accggcctct gattttccgt
     2161 cagagaacca cagagcattg atcatattta ccgtactctg tgcccccgta atactgcctc
     2221 tcagaacagc gttatatccc ttcattaagg agctgaataa cggagagtcc gtcttccctg
     2281 catcagcatc actgtctccc agattaatgg ttgccgcagt ggttcctgca tgaatatccg
     2341 aactcagata gcccatattt ttaacagaaa agctggcgtt atcttcgagg ttaatccctt
     2401 ctgttgtaga aattacgggt gagtaatacc cctgtttctg cgcagaaggc atccccgtca
     2461 gggtcagaga agcattttct tttacatcca cagccccggt gctgaaaata ccgctctgag
     2521 ccgtaagttt tgctcctttt ccgagggtca ggctgctgtt gacaaagctg ccaacacggt
     2581 caaaaacagc attctgagag gtcacactca cggtgctatc ataagcctca ataccaccgg
     2641 tgaatctctc catgatttgc agggatgatt tattttccag cgtaacattt ccgctgtaat
     2701 cactctggtc accaactgtg gttgcagtgg cactcccttc aacagaggag accgcattac
     2761 cggttccgtc actcctgtca gtaaagacac ggctacttcc cagaatgact gtacttccat
     2821 tggtggcgtt aatatctccc tgcatcttgc cgtttctgga cagatggaat gttgccgcat
     2881 cgagtttcag tttttccagc gtaaactgac gggtttccca gtcgctctgg ctggaagaag
     2941 tcgtcgttcc agcatgaata actggatgcc cctggaatat cagcgtgccg gacttcttgg
     3001 ttatcgaacc ttctggcagg ttaactgaac catcaagagc cagtacatcc ttacctgaga
     3061 gctgcggaat attcacatcc atattgccgt ttagttggcc gtgatacata taaggctggc
     3121 tgctggcctc aattttttgt tgcttcacag tatcaatggc tttattacga tcttttccga
     3181 cattctgcca gacactgctg ttgttcagat cggagaagaa cggggaatat gaactcgctt
     3241 tcaggatgaa atattgcttg gtagaactgt cataatatag gtctcccggc gctcctctcc
     3301 ccccaaaaat actgacagta ttaaccggta cattgatgtc actggctttt aatgtctgaa
     3361 ggtcgagtgt taccgttgat tttttctcac tgttattggt aatgatggcc ccgtagtcag
     3421 ccgcctgaag acgggtaaag gtcagattat taccattcag ttccagacgt cctccacggt
     3481 atccccacga aatattatcc ggattaactt gctgtgagtc agaaagcaca actgtcggac
     3541 gaccgctggc aataccaaca gagctgaaag cctgtacctt tccgtcagca tccgcctgct
     3601 ggttgagaat aacggtaccg tcccccacct tgagtccgcc tgcattcaca ccggtcccgt
     3661 ttactgtcag ggtaccttcg ccagttttat gcaggttatc accggcaact ccgttcacct
     3721 gccacagaac gtgggttcct ttatcggtga taatcccgcc ccccatccat gtctggccgt
     3781 tcagggcgga caccctgtaa tcgccggcaa actgcagata cccagctccc tgattaacgg
     3841 aattctgcag aacgacctcg ccattattgc cggtgaacag gaggtttttt cctgcgttca
     3901 gatcatttcc tttctttcca tgcatatccc aagtcgtgtt tccctgagtc agtgtgccgg
     3961 tgccattagc cgcatcatat ttccactgca gtactccttc accagaggtg taggctatag
     4021 ttttgtcaaa atcattttgc ggttgctgac caaggaaatc ctgagtggtg acgacccagt
     4081 tatttccata gttagttcct gaggaaagca cgccaatcag tacccatttt ttttgcaggg
     4141 aatcataagc aaacaggggg gagccgctgt cccccatatt cagataatta gccagaggtc
     4201 cattcgcggg attaaaaatg tcaccggtct gagctgtaat catctgtccg ttgttatagg
     4261 agttcagcgc gctgacggtt cccccaacaa gaaatccgcc attggtatac acttgggtgc
     4321 gattaccatt tttgtctttg acgtactgca tccccccccc aaggcgatag aattcagtaa
     4381 agcgtccgcc ggcctgatag gcaccgctca cagccccgat gtcagacact tctgccggcg
     4441 ccacctctgt caccagttta ctcaggcggc gggttttaat atcaagaccg ctattattat
     4501 ttgtaccaac cgcagtatag gtgtttttgg cgtatccgaa gctcatcgtg tcagaaccgc
     4561 tgacatgttt ggcggtaacc acatactgag gggagtaaag tgtatggtct cccggaggca
     4621 ggctaccggt agtaatagtg gcactactga aatcagccat cggggcttta tcaagtttac
     4681 cgaccagatt gccctgcttg tcgtaaatgg aaatattcgt ggtccccgac gtaaactgac
     4741 ctttattctc ggcaaagtcg cggaatatct gataagggat ctctgcactg accgttgctg
     4801 acagagctgg ccatgccgaa gaaagtgata aagctgcaac agacgtaaga ataacccgac
     4861 gtgaaagtct gcgatggctt ttgcaggtaa ccctgcgggc cagttcggag acagcaatca
     4921 ggcttttggt tatatggcaa tacttaagat aatatatttt gttcatatag aaacatcaac
     4981 ctaattttga caaagattaa ttacatagga tgtagatgga gcagtccgag attctactaa
     5041 tatcaattat taagcatctg ttttttagtt ggtgtaatat aatttttatt aaggtaagcg
     5101 gaaaaacaac caaaaaaaac gcaagatgtt atttttttat cattttgagc cagtttggct
     5161 ggggggatgc tcttctctta agcttcttgc tcaactttaa aaatagttat gccagttcgg
     5221 tatcgataag gggcagtttg gatatcgaga accacggcag cctgacggct gcaaattgaa
     5281 aaccgcccct gttcgtcctg ttgttgccgc acgctccgca gaatatgtat gctctgagga
     5341 cctgtttt
//
LOCUS       DAJTYI010000193         5326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYI010000193 DAJTYI010000000
VERSION     DAJTYI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..351
                     /gene="mddA"
                     /locus_tag="OO564_003773"
     CDS             <1..351
                     /gene="mddA"
                     /locus_tag="OO564_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6654703.1"
                     /translation="EEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKL
                     LSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFM
                     QLQLDERTEPDAIG"
     gene            348..797
                     /locus_tag="OO564_003774"
     CDS             348..797
                     /locus_tag="OO564_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6654704.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(798..1030)
                     /locus_tag="OO564_003775"
                     /pseudo
     CDS             complement(798..1030)
                     /locus_tag="OO564_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1116..1289)
                     /gene="ortT"
                     /locus_tag="OO564_003776"
     CDS             complement(1116..1289)
                     /gene="ortT"
                     /locus_tag="OO564_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6654705.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1484..1579
                     /gene="yncL"
                     /locus_tag="OO564_003777"
     CDS             1484..1579
                     /gene="yncL"
                     /locus_tag="OO564_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6654706.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1981..2790)
                     /locus_tag="OO564_003778"
     CDS             complement(1981..2790)
                     /locus_tag="OO564_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6654707.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3000..4425)
                     /gene="patD"
                     /locus_tag="OO564_003779"
                     /pseudo
     CDS             complement(3000..4425)
                     /gene="patD"
                     /locus_tag="OO564_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4447..5153)
                     /locus_tag="OO564_003780"
                     /pseudo
     CDS             complement(4447..5153)
                     /locus_tag="OO564_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5143..>5326)
                     /locus_tag="OO564_003781"
     CDS             complement(5143..>5326)
                     /locus_tag="OO564_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6654708.1"
                     /translation="GDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILI
                     ALYLAFVKRLGAFDAL"
BASE COUNT         1400 a         1352 c         1156 g         1418 t
ORIGIN      
        1 gaagaagatg gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt
       61 tttcgccata ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg
      121 gggcgtaaat tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg
      181 gtcgccggga tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt
      241 gtcgtcaccg cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca
      301 tttatgcagt tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc
      361 tcacccttgc ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg
      421 tgcgcatcac ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg
      481 ggattgtgct ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac
      541 tggtgtccag cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct
      601 tcgccagtat cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg
      661 ccagccagct gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac
      721 gggcgttgtt cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg
      781 ccagacgctc attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt
      841 ccatatcttc tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct
      901 gggtgtagcg atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt
      961 tcatatgggc aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca
     1021 gatgcgacat actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata
     1081 agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc
     1141 accgggaaac tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt
     1201 ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa
     1261 aaaacaagca tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc
     1321 gcgcaaatat gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat
     1381 catcaaacag caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta
     1441 acccgtacta tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag
     1501 aactgtagta ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag
     1561 atttggctgg tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca
     1621 actctctgac agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat
     1681 atttacttct ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg
     1741 tctatacaaa cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa
     1801 tactttatac gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata
     1861 aaaagatggc gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc
     1921 ccattgaaat tgcaaacccc gccctggtta tcttgcccat ttatcatgca aaacagataa
     1981 ttataggcct ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt
     2041 tccatttgat atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt
     2101 attatccgga agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat
     2161 aacatttgaa tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata
     2221 tgggggcaca tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc
     2281 gatgccacct ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt
     2341 ttcaggtttg gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa
     2401 cttatcaaat tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt
     2461 tccacccatt atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc
     2521 tgaaaaatgg caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat
     2581 tgttgaatat tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa
     2641 aagaaataaa ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt
     2701 atccatacaa aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc
     2761 ttccgtgaat ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt
     2821 ttaataacga tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat
     2881 caaacattta gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc
     2941 cattaaactc agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact
     3001 taatgtttaa ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata
     3061 tccttgccgt aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa
     3121 tgggtattga cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc
     3181 acatctttcg tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc
     3241 acctgttctt cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc
     3301 acgatggcat cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta
     3361 cccttgcgct tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct
     3421 actgccttgc tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac
     3481 tcgtcatccg gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag
     3541 cgtatcgtaa atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc
     3601 attgtaatag ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa
     3661 aacaatcact ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt
     3721 atggctgatg atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt
     3781 gggatgaccg gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac
     3841 gcctgccggg aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat
     3901 ttctgatggt ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc
     3961 ggccatcatc agcggataat tccacggtgc gatagaagcc acgcccccca acggatcgcg
     4021 acggatcatc gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg
     4081 cgccgcaccc gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc
     4141 actatgcagc ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc
     4201 ttcgataaca tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc
     4261 ccattcagca aatgctgcat ctgccgcaca cacagcagca tcgacctgct ctgcggatgc
     4321 ctcggcaatc tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc
     4381 ttcgccgcta accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat
     4441 cagtggttat tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa
     4501 ggttgttacc aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg
     4561 cccaagctga ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt
     4621 aacgatgatt tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga
     4681 actgagattc ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat
     4741 tgacgcctca accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgtcaaacac
     4801 caccactacg caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt
     4861 gatggtttta aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg
     4921 cagcagcaat aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc
     4981 agctgccagc gtccctaaca ccagcgcagc gtcaggccct gcggtggaaa actaaacgcc
     5041 gcatcttcag tgttaaacgc ataggcggcg attatcagga tgggaaaatg taggaaaaca
     5101 acgccgcccc aggccgccag tttgaggaaa aacggtgcgc gttcagagtg catcgaacgc
     5161 tcccagacgt ttcacgaacg ccaggtacag tgcgatgaga ataatcggca ccagggtgaa
     5221 tgccgccgcc atcggcatat tgccaatcgc cccctgctgg gaataaacca tattgccgat
     5281 aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa tcgccc
//
LOCUS       DAJTYI010000194         5288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYI010000194 DAJTYI010000000
VERSION     DAJTYI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..456)
                     /locus_tag="OO564_003782"
     CDS             complement(<1..456)
                     /locus_tag="OO564_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6654709.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADL"
     gene            complement(453..1328)
                     /gene="nanK"
                     /locus_tag="OO564_003783"
     CDS             complement(453..1328)
                     /gene="nanK"
                     /locus_tag="OO564_003783"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6654710.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1325..2014)
                     /locus_tag="OO564_003784"
     CDS             complement(1325..2014)
                     /locus_tag="OO564_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6654711.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2062..3552)
                     /gene="nanT"
                     /locus_tag="OO564_003785"
     CDS             complement(2062..3552)
                     /gene="nanT"
                     /locus_tag="OO564_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6654712.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3662..4555)
                     /gene="nanA"
                     /locus_tag="OO564_003786"
     CDS             complement(3662..4555)
                     /gene="nanA"
                     /locus_tag="OO564_003786"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6654713.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4677..>5288)
                     /gene="nanR"
                     /locus_tag="OO564_003787"
     CDS             complement(4677..>5288)
                     /gene="nanR"
                     /locus_tag="OO564_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6654714.1"
                     /translation="LMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTII
                     GELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSL
                     DNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQ
                     QHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1274 a         1499 c         1366 g         1149 t
ORIGIN      
        1 taaatcagcc ttaaccttca ccaccacctt tttaatctca cccggctcgc caacaacgca
       61 tcccggctta tgcggctctc ctggcataaa cacggcgaac attcccggtt tcaggatgat
      121 ggtttgctcg ttttcaatgg cgctacaaag ctggtaatca tcttcatggt ggaactcttc
      181 acattggcgc gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt ttaataacag
      241 ctggatatca atgtattgct cgtgcaattc cgcttttttc tcgacgggcg attgagtgtt
      301 aaacgtcatg acattcataa aaatattgtc gccctgtaat tcgtaacgac ccggcgcttt
      361 ttcttgcggt ctggcagcta aggccagcgt taacgcgtcc tgtaacgcag gatgtaaccc
      421 agcagacggt aatgactgta cttcacccat catcataatt tttctccctg ggccaacagc
      481 gcagccccaa gtaaacctgc atcatggcgg taatgcgccg ccagtaaatc aacatgaaat
      541 gccgctggct cctgcgccag atacatttcc accaacgcca gatacccttc tgccagacca
      601 acgctgccac caaccaccac gcactggcaa tcagttgtgg ctttaatatc agcgatcagc
      661 cttgcaagcg tacgtgcgga gcgatgaatc agttgctgcg cctgctcatc gccctgcccg
      721 gcgcgtgtga aaatagttct cgcatccgcg ccagccagct cgccctgcgc tgccgccgca
      781 atgccgcgac cagaagcaat tgcttccacg caacctgtgc gtccacagcc gcagactggg
      841 ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca gaccgccagg gccggtaagc
      901 agtttgccgc cgctcactac accgccgccg acgccagtgg aaacggtgat aaagaccata
      961 tcggttatat cgccttccag cgcctgatac tctgcccatg ctgcggcctg cgcgtcgtta
     1021 atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt tgactaacgg aaagtgtagc
     1081 aatccaccga gattatgcgg attcagcgcc agcaaactgc cgtcgcgaat aattccggtt
     1141 gaagcgatgg caacccgctg cgcatgagct tgcaacggag agactaatgc ggataaggca
     1201 tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa gttcacgacg atcgcggatc
     1261 tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc aatcgccagt
     1321 atggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac
     1381 gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca tctgccgcct
     1441 gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcttcg ctcaacgttt
     1501 tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag
     1561 tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc gttgagcagt
     1621 cggtcatcgc cagtaaaccg tgatgatgga tacgtgccag cagcgtttca acaggcaccg
     1681 gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc gccagcgcat
     1741 cgacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg cgtttcacaa
     1801 ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc acaccttcga
     1861 tgcgaatggc aaccgcgccc gcctgctctg ccgctaatgc catggctgcg acgatttcgg
     1921 gtttatcgag cgggctgtca ggaaccggct ggcaggagac aatcaggcca ccgttagcag
     1981 cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg aaaggcccgg
     2041 tacatagacc gggcaacagg attaactttt ggttttgact aaatcgtttt tggcgctgcc
     2101 aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc
     2161 cgggcgcagc caacgctgaa cgcgggaagg catatccaga ccaatcagca ggatcaccac
     2221 aaatgtcagg ctgaacgaga gcgatgccag cgcagtaccc agatccagac gttgagcgat
     2281 caacgcgccg atgattgggg ccagtgcgcc acccaatgcg ccaacgttgt aggtaaagcc
     2341 caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagtttcg gtaagatccc
     2401 ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca cccagacgtt
     2461 tgcgccgcca atcgcaaata ccggaataat cagcagctgc gaggccagca ggctacaaac
     2521 gtacgctttg tgggttccca gccagtcacc gaggaagcca cctacgcagc atcccacggc
     2581 cgcgccaaag ccactaaaga acagcacatt ggcgacagta tgcgggttat aagccagatc
     2641 ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga aagcaaacaa
     2701 cacgaccacc atcagcatta cgcccgttgg ccagcgttta cccgtacttt gcaccataaa
     2761 gctgataaag attgcggcgc ataacagccc gagaacagcg acgattgcag cattttgcag
     2821 gttaccggcg aagcagaacc acagcgcagt agccgccgcc agtgtcatta cgatattggc
     2881 aatgcgatgt tcaccacggt agagaatatc caccattgtg cgtactggtg ctttacctgc
     2941 gtgtttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga gagcaaagat
     3001 gattggcaaa atgccgataa agaacagcgc acgccaaccc cagaccggaa ccaccaggct
     3061 atagacctga gcggcaacga cggcccccac agagaagcct gaaatcaaaa aaccactggc
     3121 tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg aaccgtattc
     3181 acccgccatc cccatgccga tgaccagacg agcgataaac atggtgatgt agcctggcgc
     3241 aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca ttgccagacg
     3301 acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc gagagataaa
     3361 ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc
     3421 ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc
     3481 agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga tattctgggt
     3541 tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg gtatgaaaat
     3601 tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag gggaaaacaa
     3661 ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt cagttctggc agatattttt
     3721 catctaccgg tccaaacggt ttgcggcaca gcggcacaga aacgacatcc atataatgca
     3781 ggacagtttt caggccgcgg aatacgcccg ttttgatcag taaatcaatg actttgttgc
     3841 actcagtttg cagtttctgc gcggtctgga tatcgccttc tttcagcgcc ttcacgatcc
     3901 cctgatatcg ccagcccatg atgttgtaag tactgccgat accaccatca gcgcctgcca
     3961 gcaagccgga agcgaagatt tcgtcgtaac cgttatagag caccagatca ggatgttcac
     4021 gacggatctg ttccatctga tagagatcgc cagaagtctg tttcagcgca ccgacgccag
     4081 gcaacgtaac cagggtgttg atctgatcca gggtcagttt taccccactc agggctggaa
     4141 tgttgtacac caccatcggc aaaccatccg ctgaatcaat aattgcccga tagtgatcgc
     4201 agtgttcttc aaagctgaaa ggatagtaga acggcgtgac ggcggagacg gcatcgaagc
     4261 cataacgttt agccgatgcc gcaagttgtt ggctttcagc ggtgctgacg caaccgacgt
     4321 gggcgatgag tttaatctta cctttcgcct cttcggcgac gatttccagt acctgttcac
     4381 gctcggaaag gctttgtaca aaggcttcgc cggtcgaacc acccacgtat aaaccgtcga
     4441 tgccctgctg aatattgaac tgaaccaggc ggcgcagact cgctttatcc agtgcttgtt
     4501 gttgatcaaa aggagtcagg agtgcagcca ttacgccacg taaattcgtt gccataaata
     4561 cctctgaagt gatgcttgtc tgataaacga tataccttta tacctgttat accagatcaa
     4621 ttaagcaaca ccccatacag aaagcttata atgcgatctg cttcactaaa gtggcattat
     4681 ttctttttgt tggtggtctg accgaaagcg tgccaggtag cagagacgct gttgagatgc
     4741 gattgcaacg cacgatcggc ttcgtcagga tcatgacggc ggatcgcatc aacgatcgca
     4801 atatgctgtt gataactaac gttgttatgt tcgtgcagtg cctgatcggt aaccgttggg
     4861 cgtgcggcaa taagccagtc gagcagggca acgtggatcg ccatgaagat tgggttaccg
     4921 gggatctccg ccagcacgcg gtggaaatca acgtctgaac gaatgaatgc cgcgttgtta
     4981 tccagcgact gactgttgat ttccagtgct tttgccagca aatcgatttg ctcatcggtg
     5041 gcgtgttcag ccgcatagcg caccaggctg gattcaaaga acagacgtaa ttgttcgaaa
     5101 tgggcaatcc caccaggatg agaaaggaaa tctttcgcca tgccggaaag ctcgccgatg
     5161 atggtgtccg cagaaggtcg agagacacga gcgcgttcgc cgttgtttat ttgcaccaga
     5221 cctttgcgtt ttaacgctgc cagcgcttca cgcacagaag gacgcccgac gttaaagaac
     5281 gccatcag
//
LOCUS       DAJTYI010000195         5268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYI010000195 DAJTYI010000000
VERSION     DAJTYI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO564_003788"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO564_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6654715.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO564_003789"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO564_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6654716.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5268)
                     /gene="mdtF"
                     /locus_tag="OO564_003790"
     CDS             complement(2659..>5268)
                     /gene="mdtF"
                     /locus_tag="OO564_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR6654717.1"
                     /translation="TAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNN
                     QISGGQLGGMPQVADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVEL
                     GAEDYSTVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYD
                     TTPFIEISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAV
                     GFTINTLTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVG
                     IAVVLSAVFMPMAFMSGATGEIYRQFSITLISSMRLSVFVAMSLTPALCATILKAAPE
                     GGHKPNALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTS
                     FLPEEDQGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQ
                     GQNNGLAFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTAS
                     GFDMELLDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKA
                     EAMGVALSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNA
                     SGTMAPLSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLP
                     AGVGYSWTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVV
                     GALLATDLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAA
                     RMRLRPILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFF
                     VVVEHLFARFKKA"
BASE COUNT         1397 a         1223 c         1235 g         1413 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggt
//
LOCUS       DAJTYI010000196         5185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYI010000196 DAJTYI010000000
VERSION     DAJTYI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..609)
                     /locus_tag="OO564_003791"
     CDS             complement(<1..609)
                     /locus_tag="OO564_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6654718.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYA"
     gene            855..1481
                     /gene="gstB"
                     /locus_tag="OO564_003792"
                     /pseudo
     CDS             855..1481
                     /gene="gstB"
                     /locus_tag="OO564_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1478..2592)
                     /gene="yliI"
                     /locus_tag="OO564_003793"
                     /pseudo
     CDS             complement(1478..2592)
                     /gene="yliI"
                     /locus_tag="OO564_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2703..3086)
                     /gene="bssR"
                     /locus_tag="OO564_003794"
     CDS             complement(2703..3086)
                     /gene="bssR"
                     /locus_tag="OO564_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6654719.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3299..4624
                     /gene="rimO"
                     /locus_tag="OO564_003795"
     CDS             3299..4624
                     /gene="rimO"
                     /locus_tag="OO564_003795"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6654720.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4671..>5185)
                     /locus_tag="OO564_003796"
     CDS             complement(4671..>5185)
                     /locus_tag="OO564_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6654721.1"
                     /translation="VLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKAL
                     PEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFA
                     VIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNKCTTVERLKMLADERLYKSK
                     KNGRAQISWQ"
BASE COUNT         1298 a         1344 c         1252 g         1291 t
ORIGIN      
        1 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
       61 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      121 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      181 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      241 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      301 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      361 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      421 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      481 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      541 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      601 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      661 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      721 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      781 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
      841 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
      901 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
      961 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1021 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1081 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1141 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1201 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1261 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1321 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1381 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1441 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1501 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1561 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1621 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1681 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1741 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1801 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     1861 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     1921 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     1981 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2041 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2101 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2161 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2221 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2281 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2341 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2401 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2461 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2521 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2581 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2641 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2701 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2761 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     2821 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     2881 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     2941 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3001 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3061 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3121 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3181 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3241 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3301 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3361 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3421 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3481 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3541 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3601 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3661 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3721 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3781 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     3841 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     3901 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     3961 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4021 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4081 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4141 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4201 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4261 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4321 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4381 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4441 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4501 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4561 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4621 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4681 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4741 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4801 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     4861 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     4921 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     4981 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5041 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5101 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
     5161 aagaaaagcc cgccatccag tacgc
//
LOCUS       DAJTYI010000197         5128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYI010000197 DAJTYI010000000
VERSION     DAJTYI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..427
                     /locus_tag="OO564_003797"
     CDS             <1..427
                     /locus_tag="OO564_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6654722.1"
                     /translation="NPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPL
                     GVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL
                     GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            761..3040
                     /gene="ydeP"
                     /locus_tag="OO564_003798"
     CDS             761..3040
                     /gene="ydeP"
                     /locus_tag="OO564_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6654723.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3288..3485
                     /gene="safA"
                     /locus_tag="OO564_003799"
     CDS             3288..3485
                     /gene="safA"
                     /locus_tag="OO564_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6654724.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3559..4320
                     /gene="ydeO"
                     /locus_tag="OO564_003800"
     CDS             3559..4320
                     /gene="ydeO"
                     /locus_tag="OO564_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6654725.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4722..>5128
                     /locus_tag="OO564_003801"
     CDS             4722..>5128
                     /locus_tag="OO564_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR6654726.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1479 a         1095 c         1130 g         1424 t
ORIGIN      
        1 aaatccgcgt aattttacgt gtaatattat ttcaaataac agtgttgtta tgccaacagg
       61 cggctgcacc gttgatagtc gtaatgtcac cgtggatctc ccggactttc ctggaagcgc
      121 agaaataccc cttggcgtat attgctcaag cgaacaaaag ctctcatttt atctttcggg
      181 tgccaccact gacagtagcc gacaagtgtt tgctaacaca gcacctgatg caacaaaagc
      241 atcgggagtg ggtgtcactc taatgcgtaa tggaaaaata ctggctacgg gagaaaatgt
      301 ctccctgggg acagtaaata aaagcaaagt acctctgggt ttaagcgcaa cctatggaca
      361 aacaggaaac aaggtttcag ccggaaccgt ccagtcagtc attggtgtca cttttatcta
      421 tgaataattg tgattttgac cttagtctat gcgctccatt cacgaaaatt gcgcggataa
      481 ttattttgtg aaggctatta tcctacacct gtaagaaaat ccgcgcacaa ttgaagccag
      541 agtcttattt tttatattga aaaatatgtt aatttaaaca ctatgagaga tgatgcttaa
      601 cttatcgtat ttaatctatt gtttaacgat aatagattaa aaatagaaag agagtagtcc
      661 cctactctta tatatccatg ttggcgataa tccctttgta tgtactgtgc atcatcgcta
      721 ttacaaatcc taataattca tttccacaca ggataagtag atgaagaaaa aaattgaatc
      781 ctaccagggt gctgcaggtg gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa
      841 gcagatggat atacgccatg atgttattgc catgtttgac atgaataagc cagagggctt
      901 tgactgtccg ggttgtgcat ggccagatcc taagcacagt gcgtcattcg acatttgtga
      961 aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg cctctttctt
     1021 tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg aggctgcggg
     1081 gcgtctcact cagcctttga aatatgatgc cgtcagcgac tgttacaagc cattaagctg
     1141 gcaacaagct ttcgacgaaa ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt
     1201 tgaattctat acttcgggcc gcacttccaa tgaagctgcc tttctttatc agctttttgc
     1261 ccgtgaatac gggagcaata actttcccga ctgctccaac atgtgccatg aaccgacaag
     1321 cgtgggtttg gcagcgagta tcggtgtagg taaagggacc gtgttgctgg aagactttga
     1381 gaagtgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc ctcgcatgct
     1441 gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgccatca atcctctaca
     1501 ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc
     1561 tgagactcag ttggccagtg cctactacaa cgtgcgcatt ggtggcgata tggcgttgct
     1621 caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagtgccg caggtcggcc
     1681 ctcattgctt gatgacgaat ttattcaaac gcataccgtc ggctttgacg agctacgccg
     1741 tgacgttctc aattccgagt ggaaagatat cgaacgtatt tctggactaa gtcagacaca
     1801 aatcgccgaa ctggctgacg catatgccgc tgccgaacgc accattatct gttacggaat
     1861 ggggatcact cagcacgaac atggtaccca gaacgtgcag caactggtca atttgctgtt
     1921 gatgaaaggt aacattggca agcctggtgc gggtatctgc ccactacgtg gtcactctaa
     1981 tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt ttctggctcg
     2041 cctgggtgag cgctatggct tccccccacc tcatgcacct ggacatgctg caattgccag
     2101 catgcaagca atatgtacgg ggcaggctcg agcattgatc tgcatggggg gcaactttgc
     2161 actggcaatg ccagatcggg aagcgagcgc tgtaccgtta acgcaattag atttggcggt
     2221 acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata gctatattct
     2281 gccggttctg gggcgtagcg agattgatat gcaaaaaagc ggtgcgcagg cggtaaccgt
     2341 tgaggattca atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat
     2401 gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag gcagcactac cccagagcgt
     2461 ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt
     2521 gctgccagag ttcgccgact ataaccagcg catccgtcat cccggtggtt ttcacctgat
     2581 aaatgcagct gctgaaaggc gctggatgac gccgtcaggt aaggctaatt tcattaccag
     2641 caaagggctg ttagaagatc cctcttcagc gtttaacagt aagctggtca tggcgacagt
     2701 acgcagccac gatcagtaca acacgacgat ttatggtatg gatgatcgct atcgaggggt
     2761 attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa
     2821 aaacggcgaa agagttaatc ttattgcgct tacgccagac ggtaagcgca gctcacgccg
     2881 catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg tgacctattt
     2941 tccagaatcg aatcacatgc taacacttga taaccacgat ccattaagtg gcattcctgg
     3001 ctataaaagt attccggttg aattagaacc atcaaattaa tgcctcttct catttcttct
     3061 gctgtcatcc gcacagcaga agaattcctc attgactatt attgcgcaat ttactcacat
     3121 ggattaaatt aaactacata ctataagata taaacttctg cctacagctg taagaaactc
     3181 cgctcagtac tgaagcacca gtcctatttc ttcttttctc cagcctgtta tattaagcat
     3241 actgattaac gacttttaac gttatccgct aaataaacat atttgaaatg catgcgacca
     3301 cagtgaaaaa caaaatcatg caaagagata actataaaga aatcatgtct gcaattgtgg
     3361 ttgtcttatt actgacactt acgttgatag ccattttttc ggcaattgat cagctgagta
     3421 tttcagaaat gggtcgcatt gcaagagatc ttacacattt cattatcaat agtttgcaag
     3481 actgaaaata aacagcaaat tataaatgtg aaatgtttaa aaggtatcga taaaacttta
     3541 ttgttttaag gagataaaat gtcgctcgtt tgttctgtta tatttattca tcatgctttc
     3601 aacgctaaca ttttagataa agattacgcc ttctctgacg gcgagatcct gatggtagat
     3661 aacgctgttc gtacacattt tgaaccttat gagcggcatt ttaaagaaat cggatttaat
     3721 gaaaatacca ttaaaaaata tctacaatgc actaacatcc agacagtgac ggtgcctgtt
     3781 cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat tgcttaatga aatgattgct
     3841 tatctcaact cggaagaacg caatcatcat aatttttcag aacttttgct tttttcttgc
     3901 ctgtctattt ttgccgcatg caaaggtttc attacactat taactaacgg tgtgctatcc
     3961 gtttccggga aagtgagaaa tattgtcaac atgaagctgg cgcacccatg gaagctgaaa
     4021 gatatttgtg actgcctgta catcagtgaa agcctgttga agaaaaaact aaagcaagag
     4081 caaacgacat tctcacagat tctattagat gccagaatgc agcacgcaaa aaatttgata
     4141 cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg gttatgccag tacatcttat
     4201 tttatttatg cgttccgcaa acatttcggc aacagtccga agagagtttc taaggagtac
     4261 cgttgtcaaa ggcacacggg tatgaatacg ggcaacacga tgagtgcttt agctatttga
     4321 ttatttgcta acgagtagtc aaccacgcac gctacgtaag aattatatgg ggcagccatt
     4381 ccctgccccg cgttgttttt aggcgatata tttattgaaa taaataagtg acatccatca
     4441 catatttatg cacttgcata acctgttgca tgattattta taatctcaat tcggcatttt
     4501 gtcagtaaaa tgcaataatt tattaaatat caataaatta gttatttatc ggcgagaaat
     4561 tacttaatag aacagaaagt aatgtcaacg ctttatggac tgttatttcc ctttttttag
     4621 ctaaatctgc tatctcttta tgtgactaac ttcacttaca tccacttatt tctcttcgta
     4681 aaattacttc ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga
     4741 aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg
     4801 ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact
     4861 ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtacta accatggatg
     4921 atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa
     4981 atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcacaaa
     5041 aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg
     5101 tggcacacgg tgtttccggc ccctcccg
//
LOCUS       DAJTYI010000198         5115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYI010000198 DAJTYI010000000
VERSION     DAJTYI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..586
                     /locus_tag="OO564_003802"
     CDS             <1..586
                     /locus_tag="OO564_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6654727.1"
                     /translation="GYRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHE
                     GWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYT
                     SHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFD
                     YIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            587..844
                     /locus_tag="OO564_003803"
     CDS             587..844
                     /locus_tag="OO564_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654728.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            884..1424
                     /locus_tag="OO564_003804"
                     /pseudo
     CDS             884..1424
                     /locus_tag="OO564_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            1491..2909
                     /gene="dcm"
                     /locus_tag="OO564_003805"
     CDS             1491..2909
                     /gene="dcm"
                     /locus_tag="OO564_003805"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6654729.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2890..3360
                     /gene="vsr"
                     /locus_tag="OO564_003806"
     CDS             2890..3360
                     /gene="vsr"
                     /locus_tag="OO564_003806"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6654730.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3349..4269)
                     /gene="yedA"
                     /locus_tag="OO564_003807"
     CDS             complement(3349..4269)
                     /gene="yedA"
                     /locus_tag="OO564_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6654731.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4442..>5115
                     /locus_tag="OO564_003808"
     CDS             4442..>5115
                     /locus_tag="OO564_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HCR6654732.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIA"
BASE COUNT         1268 a         1241 c         1444 g         1162 t
ORIGIN      
        1 cggataccga agcccgcggg cacgtaaagg cgaggccagt atcgtgtcac ccaacaggct
       61 ccagcgacag ttcaatccgg atgctcctga tgagcgttgg gtaacggaca taacctacat
      121 caggacccac gaaggctggc tgtatcttgc cgttgttgtt gatctgttct cacgcaaaat
      181 tatcggctgg tccatgcaat cccggatgac aaaggacatt gtcctgaacg cactgctgat
      241 ggctgtatgg cggcgtaatc ccgaaaaaca ggtgctggtt cattcggatc agggcagtca
      301 gtacacaagc catgagtggc agtcgttcct gaaatcacac ggcctggagg gtagcatgag
      361 ccgtcgcggt aactgccatg ataatgcggt tgcagaaagt tttttccagt tgttgaaacg
      421 tgaacggata aagaaaaaga tctacggaac gcgggaagaa gcccgcagtg atatttttga
      481 ttacatcgaa atgttttata acagtaagcg tcggcatggt tctagcgaac agatgtcacc
      541 gacagaatat gaaaaccagt attatcaacg gctcggaagt gtctagatta tccgtggcga
      601 ttcattgtgg gctttctata catggtgtgg ctatttcgtc tgggcaatgg cgcgatacat
      661 ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga tttgaaagtg atttaggatc
      721 taccgcgggg aagtggttag gagcattggt cggatttctg tttatggccc tggttggtgc
      781 cttgttaggc ggcattgcat ggtacacccg acctcgtccc gcacgttgca gacgctatga
      841 ataaaaactg ctaaggttgc gactctttgg caacggagcg cgtatggagt tacaacactg
      901 gcaggcacag ttcgagaact ggttgaaaaa ccatcatcaa catcaggacg cggcgcatga
      961 cctgtgtcat tttcgccgtg tctgggctac ggcacagaaa ctggcggcag atgacgatgt
     1021 cgatatgctg gtgattttaa ccgcctgtta ttttcacgat atcgtgagcc tagcgaaaaa
     1081 tcatccgcaa cggcagcgtt cttcaatcct ggcggcagaa gagacgcgtc gcctgctccg
     1141 tgaagagttc gtgcaatttc cggcggagaa aattgaggcc gtttgtcatg ccattgccgc
     1201 tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa gctaaaatcg tccaggatgc
     1261 ggatcggctg gaagccctgg gggcgattgg cctggcgcgc gtatttgccg tttcaggggc
     1321 attgagcttg gcgctgtttg atggcgaagc tccgtgccga actggcgggg gagaatgaag
     1381 gtatcgatca caaggtgata gatgcgtttt cacccgctgg ctgagcgcgt ggccctaaac
     1441 ggcagtaatt atgttaacct gtcggccatc tcagatggcc ggttaaatct atgcaggaaa
     1501 atatatcagt aaccgattca tacagcaccg ggaatgccgc acaggcaatg ctggagaaac
     1561 tgctgcaaat ttatgatgtt aaaatgctgg tggcgcagct taatggtgtg ggtgagaatc
     1621 actggagcgc ggcaatttta aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa
     1681 gtgagaaaga gtttgcccat ctgcaaacgt tgttacccaa accaccggaa catcatccgc
     1741 attatgcgtt tcgctttatc gatctatttg ccggaattgg cggcatccgt cgtggttttg
     1801 aatcgattgg tggacagtgc gtgtttacca gcgaatggaa caaacatgcg gtacgcactt
     1861 ataaagccaa ccattattgc gacccggcga cgcatcattt taatgaagat atccgcgaca
     1921 tcaccctcag tcatcaagaa ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac
     1981 acattcctga acacgatgtt ttactggccg gtttcccttg tcagccattt tcgctggctg
     2041 gcgtatcgaa aaagaactcg ctcgggcggg cgcacggttt tgcctgcgat acccagggca
     2101 cgctgttctt tgatgtggtg cgcattatcg acgcgcgtcg tccggcgatg tttgtgctcg
     2161 aaaacgtcaa aaacctgaag agtcacgata agggaaaaac gttccgcatc atcatgcaga
     2221 cgctggacga actgggctat gacgttgccg atgcagaaga taacgggccg gacgatccga
     2281 aaatcatcga tggtaaacat ttcctgccgc agcaccgtga acgcatcgtg ctggtgggtt
     2341 ttcgtcgcga tctgaatctg aaagccgatt ttactctgcg tgatatcagc gaatgtttcc
     2401 ctgcacagcg agtgacgctg gcgcagctgc tggacccgat ggttgaggcg aaatatatcc
     2461 tgacgccggt gctgtggaag tacctctatc gatatgcgaa aaaacatcag gcgcgcggta
     2521 acggcttcgg ttatggaatg gtttatccga acaatccgca aagcgtcacc cgtacgctgt
     2581 ctgctcgtta ttacaaagat ggcgcggaaa ttttaatcga tcgcggttgg gatatggcca
     2641 cgggtgagaa agactttgac gatccgctga atcagcaaca tcgtccacgt cggttaacgc
     2701 ctcgtgaatg cgcgcgctta atgggttttg aagcgccggg agaagcgaaa ttccgcattc
     2761 cggtttcgga cactcaggcc tatcgccagt tcggcaactc ggtggtcgtg ccggtctttg
     2821 ccgcggtggc aaaactgctt gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag
     2881 aggcacaaca tggtcgacgt tcacgataag gccacccgca gcaaaaatat gcgcgcgatt
     2941 gccacgcgtg atacggcgat tgagaagcgc ctcgccagtt tgttaaccgg gcagggcctt
     3001 gcgtttcgcg ttcaggacgc cagtctgccc ggacgcccgg attttgtcgt tgatgaatat
     3061 cgctgcgtga tatttaccca cggctgcttc tggcatcatc atcactgcta tctgtttaaa
     3121 gtgcctgcga ctcgaactga gttctggctg gagaagatag gtaaaaatgt tgaacgcgat
     3181 cgccgcgata tcagtcgctt gcaggagctc ggctggcgcg tactgattgt ctgggagtgt
     3241 gcgttacgta ggcgcgagaa gctgacggat gcggcgctta ccgagcgtct ggaagagtgg
     3301 atctgcggcg aaggtgccag cgcgcagatc gacacgcagg ggattcattt actcgcttga
     3361 tgcatcctga ataactggcg cgacaacggg tttggccggg aagagatatt ttcccaacgt
     3421 gaccagtacc accgcgaaaa caattacgcc gagcgccagc cattcaatct tcgacagcgt
     3481 ttcgccaccc agtcccgtac ccagcaagac cgcgaccacc gggttaacgt aggcgtagct
     3541 ggtggcgaga gccggactga cattacggat taaatacata taagcgttaa tggcgataat
     3601 cgaaccaaac agcgccagat agccgaccgc aaggaagcct gacagggaag ggagcgccgt
     3661 cagtttttca cccgcaatca tcgatgcgat cattaacacc acgcctgccg ccagcatctc
     3721 aatcgcaccc gccatcatcc ctacaggtaa ggtaatgcgc gagccataaa ctgagccaaa
     3781 cgcccagcta atcgagccga ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt
     3841 tccaccgcta ttgagcatga tgattccggc aagcccaatg gcaataccca cccattccag
     3901 tttgcgcgtt ttaatgccaa acaggcgact gaagcacagg gtaaagaggg gcacggttgc
     3961 aatcactacg gcggcgatgc cggaaggaac attttgatgt tcggcaaccg tcaccatgcc
     4021 attaccgaca gccagcaata acaggccaat cagcgcggca ttgagcagcg gacgtagcgg
     4081 gggaagtttg tgtccgcgca gtagcaaaaa tgccagcaat aaaataccgg ctgccaggaa
     4141 tcgaacgccc gccatcatta acggaggcca gcttcccacg ccaatccgaa tgacaaaata
     4201 ggttgagccc caaatgatat acaacgcaaa cagcgcgcca aaaagcggta acaactggcg
     4261 gaaacgcata atccctcacg gcggaaataa aaaggtggtt catagtaaac gtgaaaatca
     4321 ttctgctggc tagagatata attgcacttg attgttaaat aaatgttgac ctgtgaagca
     4381 agtatcagag ctgccgtttt tgcttcatac ttacaccctt cagcaataaa aatgagaggg
     4441 aatgcttttg gccgggagta gtttactgac gttgctcgat gatatcgcca cactgctgga
     4501 cgatatctct gtgatgggca aactggcggc gaagaaaacc gccggtgtat taggggatga
     4561 tttatcgctc aatgcgcaac aagtttcagg cgtgcgggcc aaccgggaac ttcccgtggt
     4621 ctggggcgtg gcgaaaggat cgctgatcaa taaagtgatt ctggtgccgc tggcgctgat
     4681 catcagtgcg tttatcccgt gggcgattac gccactgttg atgattggtg gcgcgtttct
     4741 ctgctttgaa ggggtagaga aagtgctgca tatgctggag gcgcgtaaac ataaagaaga
     4801 tccagcgcag agccagcagc gtctggagaa gctggcggcg caggatccgc tgaagtttga
     4861 aaaggacaaa attaaaggga cgattcgtac cgattttatt ttgtctgcgg aaatcgtcgc
     4921 catcacgctg gggattgtgg cagaggcgcc gttgcttaat caggtgctgg tgctttcagg
     4981 catcgcgctg gtagtgaccg tgggcgtcta cggtctggta ggggttatcg ttaagattga
     5041 tgacctaggg tattggctgg cggaaaaatc cagcgcgctg atgcaggcat taggtaaagg
     5101 gttattgatt atcgc
//
LOCUS       DAJTYI010000199         4930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYI010000199 DAJTYI010000000
VERSION     DAJTYI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(399..1850)
                     /gene="uxaB"
                     /locus_tag="OO564_003809"
     CDS             complement(399..1850)
                     /gene="uxaB"
                     /locus_tag="OO564_003809"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6654733.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(2077..3495)
                     /locus_tag="OO564_003810"
     CDS             complement(2077..3495)
                     /locus_tag="OO564_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6654734.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGVGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3634..4026)
                     /locus_tag="OO564_003811"
     CDS             complement(3634..4026)
                     /locus_tag="OO564_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6654735.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            complement(3993..4919)
                     /gene="glsB"
                     /locus_tag="OO564_003812"
     CDS             complement(3993..4919)
                     /gene="glsB"
                     /locus_tag="OO564_003812"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6654736.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
BASE COUNT         1335 a         1300 c         1170 g         1125 t
ORIGIN      
        1 ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg gctgaagaaa
       61 aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct aagtgtgccc
      121 gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac catgcagaga
      181 gagaaaacac cggagtggag ggaaaagcag aaaagctcac gtggcataag gcgtggacag
      241 cggtatcgtc tggtttttca atttcctata tgtcaagctt acaacattaa caggcgctgc
      301 atgtgacacc tgttaatgat aaaggcatat aggatgttgt aactaatata gtgaaataag
      361 aaacccggtc gaaaccgggt tcagaagtaa cggtgttatt agcacaacgg acgtacagct
      421 tcgcgcatcc ctttttcaag aatcgcatcc aggtcattag caacctgctc gaccagacca
      481 ggcacttgcg tcaggtcctg ctcccagtgg tctttttctg ccaacacaat cgctaccagt
      541 tcctgggtac cgataacacg gtcacgatgc tggctccaga gttgctgata acgttccagc
      601 cagtgtgcat cgtcctgtac cggatatgtt tctccgttac gctcaccacg atagaacgca
      661 atcaatgccg ctaatgcgaa agtaaggcgc gccggaagtg tgccgtttgc cttctgccct
      721 gccagcagct gcggcaggat gcgggtgcgg aacttggtca taccgttgag cgcgatagac
      781 agcagctgat gcttaatgta cgggttacgg aaacgcccgg tgactgcact ggcgaaagat
      841 tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat ggctttttca
      901 acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag ccctgcctgg
      961 aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt gcgttcttta
     1021 tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag gcgcagctca
     1081 gtcgctaagg atttcggccc ctgaatcaca aacaggtaaa aatgttcagc ggtgtcgagg
     1141 aaaccatcgt gataacccag ttcttcttcc agtttagcca cttcatcgcg cggataaccg
     1201 gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata
     1261 aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg
     1321 ccattgtagt caatcaactc acacggaatg atgatccaac ctttatccag cgcaccgttg
     1381 aaatggctaa agcgttcgaa cagcagacgg gtcagttttg ccggatagct taccgctggc
     1441 gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt gttagagaaa
     1501 acaaagcgca tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg
     1561 ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca
     1621 ttcaggccac ggataatggt ggtgtacaga ccatcctgcg tgctcagtga cggcgggaat
     1681 gaagtttcaa tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg
     1741 agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata
     1801 cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc
     1861 cttctgatta gtcatacaac ctgtttgaat tggtgcgaca ggttagcaaa ctttaatacg
     1921 ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg
     1981 gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa gtggtagaac
     2041 acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct caccgtaacg
     2101 catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg tatcggcttc
     2161 gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac cactaccgac
     2221 gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt gcagttcaac
     2281 gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg acggcactgc
     2341 aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga caatcttctg
     2401 aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat agttatcgtt
     2461 aatgcttttg aaatagtcga tatcaagcag catgaccgtc agatgttgtg tctgtttcag
     2521 cgatggactt ttcagcgcct cataaagacc ggaccgtgag taaacctgag tcagaaaatc
     2581 aaagtcggct cgcagcgcaa cttgcttcat tagcaaattg atcgctgcca cgctaaaaga
     2641 aaccataatt gggcatatcg ccatcgtggc aataccgaga cgtgcggaga acatttccgg
     2701 aatggagaac ggcgaaccga ccgagatatc aatcaccgaa tttgccacca gtacgatttc
     2761 caccgcaccg gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc gcactgcaca
     2821 ccagattaat gcaggcagcg ggaacgccag actccccact ccgccaatga ctaccgaggc
     2881 aataaccgac acaataagcg cgatggcggg catcatctgt tctgctttaa agcgcggcag
     2941 cactccagga atagccaacg tcagcataca aggcacgatc aacacgcccg ttgagaattg
     3001 ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac tgaccgaacc
     3061 aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa atagccgtaa
     3121 ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga ccagtaaggc
     3181 gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca gtccccattc
     3241 ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt aatgcagtcg
     3301 attaagccag acatagcggg caaacacccc cgccattacg ccattcagcg gccagaaaag
     3361 agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg tggttagcgc
     3421 aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga aagttgaaat
     3481 ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta tctcaacgtg
     3541 ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa taaaggtacc
     3601 ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct gtacaaccag
     3661 ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg aaacaccctg
     3721 cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg cgtgctgagc
     3781 gataaacacc ggatgcccgc gcatgggggg ttgcttaccg tcattagccg gtaatgccgg
     3841 agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc
     3901 caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga
     3961 taaacgcgcg aagagtggat cgagagactg ccttaataaa ccgaacgccc taactgtttc
     4021 gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga
     4081 ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca
     4141 gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg
     4201 gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg ttcatcaata
     4261 tgaatagctt tcccctgatt agccagaaag acaaacgtcc gggccagctc tacacagctc
     4321 attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa
     4381 ttgccaaacg acttcatcag ccaagcgata gccgcatttc gcgcggaatg ttcaaattcg
     4441 gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact
     4501 tccagcatac gttgccgtgg tgcgcttagt cgcccttgca acatatcgca gaccaccagc
     4561 gcaccggcat taatgaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa
     4621 gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag
     4681 taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt
     4741 tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatccccaat
     4801 cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg
     4861 agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact
     4921 ttctactcct
//
LOCUS       DAJTYI010000200         4925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYI010000200 DAJTYI010000000
VERSION     DAJTYI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.202"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1139)
                     /locus_tag="OO564_003813"
     CDS             complement(<1..1139)
                     /locus_tag="OO564_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6654737.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGG"
     gene            complement(1139..1321)
                     /locus_tag="OO564_003814"
     CDS             complement(1139..1321)
                     /locus_tag="OO564_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654738.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(1333..3090)
                     /locus_tag="OO564_003815"
     CDS             complement(1333..3090)
                     /locus_tag="OO564_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6654739.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(3090..3572)
                     /locus_tag="OO564_003816"
     CDS             complement(3090..3572)
                     /locus_tag="OO564_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6654740.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(3720..4070)
                     /locus_tag="OO564_003817"
     CDS             complement(3720..4070)
                     /locus_tag="OO564_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6654741.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4113..4577)
                     /locus_tag="OO564_003818"
     CDS             complement(4113..4577)
                     /locus_tag="OO564_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6654742.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4574..4925)
                     /gene="rrrD"
                     /locus_tag="OO564_003819"
                     /pseudo
     CDS             complement(4574..4925)
                     /gene="rrrD"
                     /locus_tag="OO564_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT         1099 a         1444 c         1177 g         1205 t
ORIGIN      
        1 ccaccgggac gcggattcat atcttccagg tcgcggcagt cattgggaga gtaaatcccc
       61 cagttaatcc cggtggcgta ggcttcaaaa cgggatttca tatccccgcg cagtaacgcc
      121 ccggcgttaa atttggcgta aaacttcccc tgtttgctct ccctgaccag ccctgtattg
      181 atccgttgtt caatacgggt cagatacggc acaagggaat agttgataaa tccgagcccc
      241 agctcttcaa tattgttgaa agtggcgcga tcggtgttct gcaccatgtg cagcggcacg
      301 cggaaaagac gacagatttc ttccaactga aacttgcggg tttccaggaa ctggctgtcc
      361 tcggcattca gtgccaccgg cttccagtcc agccccattt ccagaatcat cggacggtgc
      421 gcattaccca gcccaagatg acgctcctca aaatccctct tcatgcgctc ataagcatcc
      481 ggcgtgagct tttgttccgt gcgcaacaca ccggatgtca ccgcaccatt accaaacaac
      541 ctggcaccgt gctcctcggt tgccgctgcc agtgaaatgg cctcgcgcgc atatgcaatg
      601 ggattcagcc cgaccagtcc gtccagcgtc agggtgcgca catgccagat ttcatcctgg
      661 gtcaacacat ccacggaacc atccggaaac gtcacctgat aaaccggctg ccactggctg
      721 ttcagcttcg gttccacaca gcccggatct atcggaagaa gctccacaac ttcccccagc
      781 gccttcacct tgtaagcgta aaaattcccc cgcagacaca ggcagacgat aaccagctcc
      841 cagaattcct gcggcgtcat gtagccattg ggttttgctg aaatcagctt atgcagccgt
      901 tcatccaccg cccgtgtttt aagggtgccg ctgattttgt agagactgca gggcagcata
      961 ccaacagact cagccagcac cctgacgcag gaatagaccg ccgtcagccg catggcccgc
     1021 tggctgctga tccgctttcc ggtataggtg tcgtatgaca accccagctc ttccgcaagc
     1081 atcccgggcg ttgtgacggg ggtgttattt ttgcgttgaa aaagcccctg gaaaaacatt
     1141 actcacctcc ggaggcgacc cggtgaccgc gatcgagata acgcgcaacc agccacgacc
     1201 agcacagaca cagcgccccg gcaacaacaa aacctgccgg gggataaatc agccatgtgc
     1261 catacgacag caaaagcgcc cccagcacgc ccaccagtgg cgtgagaatt atcagaaaca
     1321 taatgacctc ggttaaagcg agcgaatacc aacgctgacc agatgctcag acagatccgg
     1381 ctccggttca ccgccattga ccagcatccg gctcattgct gtaaacatcg caacagggcc
     1441 gtcgattttg gcttccggcg tggatttgtt cgggaagata ttgtcgtttt tgtccggttt
     1501 caccgtaacg ttagacatca tccagttcat gaccgggtga ttactgtgat ggaaacgccc
     1561 ggcatagacc agtgattccg tttccttcat ggcctctgac agattgcgga ccgtctgcgg
     1621 aacttccacc agcggtatac cttcttcagc cagcgccaga ctgaactgca ttgcgctcca
     1681 cgggtcaaat cccagttccc tcaggttttc accgccaatc cattccagta agtcactttt
     1741 tatctgagca tgatcgataa catcaccatc cgtcagaatc agcttatcca tctccgccca
     1801 cttccggtaa agttctgcct gctgccgcga gcatcgttcc agccgtcctt ccggaagcca
     1861 gaatttaaaa tcggcatgaa catgcccgtt atccgttcgc cagagttttg ccgccgcaca
     1921 gatatcaatc ttatgagcaa ggtcaacgcc gacccacatg ggatacgttt tcagctcatg
     1981 tcgtggggca atgtattcgc acttctccca cttaatcatg tccatccagg cagactctgc
     2041 tgttacccac acattcatgt gtttggtaaa aaaattcacc cgcgcagaga cctgttcttt
     2101 cgcttttttc gccaggcgac gcagatcatc ccagcgttta cagatgccca ggccgggatt
     2161 cgctttctgc cagaccgttt catcaaacgg atcatctccc tcatcgaggg tgtaaataat
     2221 cgcaaagtag gagtcgtctt ttaccgcgcc ctccacgtcg ctgttatagc cacgcaatac
     2281 cttgatggcg taatcacgct gctcgtaaca aattccttcc ttgttaaacc ctgccgtggt
     2341 gataccaaat aaaagggact gcagacgggc accggttgcc gtttccagaa cgtcccacac
     2401 gtcacgggtt ttatgtgcat gcagctcatc aataatggcg cagtggatgt tcagaccatc
     2461 caggttgttt gcatctgaag aaagcggttc aaattttgat gcgctctgct cctgataaat
     2521 cgccagcttg ttgaaatcaa acaaccgccc gagtgtcgac cgggcttttc tgaccatatt
     2581 tttggcgtct tcaaacacga ttctggcctg gtcacgcgtg gttgcggctg aatacacctc
     2641 agccccgcct tcaccatctg cccccgtcat atacagaccg atacctgctg acagggttga
     2701 ttttgcgttt ttacgggcga cttcgttgta cgctgtccgg aaacggcgca ccatcaccgg
     2761 gcgtccgctg ccatcgctgc gcatgacaac ttccccggtc tcttcattca ccagcggaat
     2821 gacaaaacca aaaatattaa tgaggataaa cacatgccag tccatcaact caatgggctg
     2881 acctgccagc gcccctttta catggggcac aaatttgtag aaattcagga tgtgctgcgc
     2941 acggggttca ctgaaataaa tcccccgctc ttcgccgtac ttcagatcat caagaaaacg
     3001 ctggcaggcc agacggacaa attcaccagc gacaatttct cctgcaacaa cacgttcggc
     3061 gtagcggatc ccgtcagcca cttttgccat cagtctctcg cttttaaaag ttctgccagc
     3121 ggatcaacat catccggtcc ggcgatattt actttcgccc ggcttgccgg tgacatacca
     3181 aattctgcaa gcatcgcccg gatccgcttc caggcatccg ctttcatcgc agcagccggg
     3241 tgtgccttaa tcagcacatc accgttctgc gtttccgtgc ggtaggtata accctcaaca
     3301 tcgagggttt cgcagtgatg ccggtattcg gtgtaggctt ccaccagcaa ctcgagtgca
     3361 cgcgcatcaa gctgagaaat gatcccttcc gcattcagct cttccgccat tcgcctgaac
     3421 cagtactttc cctgcgcccc taaatgctgc ggaattttag ggagaccttt ttcgtccttt
     3481 ttagcggttt ttttggcgtc tttaacggga cgctttgagg gattgcctcg tatcaaatgc
     3541 aggcgtggcg gggttttcgg gggtcctgac ataatcgatt ttacctatca atcgcttgat
     3601 cgcattccca aaaaaaagtt ttcgaacctg cggcgatgcg aggaagggtt ggcgggcggt
     3661 cccggacagc cagggctgca gggatttgac ccgcccctcc ctacaagtga gaataattat
     3721 cacctgattc gttcgcgcgc tgttttcgct ttatggcagg gccagcacag actctgcagg
     3781 ttgctgtctg cgtctgttcc gccatgcgct ttcgggatga tgtggtcgac ggttttcgcc
     3841 tcgctcacca caccgacacg cagacacaac tgacacagac ctttatcgcg cttcagaata
     3901 cgggcacgaa tcaccgtcca ttttgagcca tagccacgct ggtggcggct cagtccgcgc
     3961 tggtgctgca cccagccttc accgcgatgc ttatcgcagt aacccgaact gtctgtggtt
     4021 gtacctgcac atccacgctt acggcaggca cgtgggatta gtgatggcat aaatacctca
     4081 taccctgcga aatgtttacc acgataaaaa ggctacttaa tgcactgagt gcggatatac
     4141 tcctgtgccc cttccagttg catctgcatc gtcatcagcc gctctctgag ggtgaaataa
     4201 tcccgttcag cggtgtctgc cagtcggggg ctggttgcat tatccacgcc ggaggcggtg
     4261 gtggcttcac gcactgactg acagactgct ttgatgtgca accgacgacg accagcggca
     4321 acatcagcgc gcagagtttc attttcagct ttcgcatcag ctaactcctt cgtgtattta
     4381 gcatcgagtg cattaacatc acgctgtctc tgctgcatgt cagtaatggt tgcgttcgcc
     4441 agcttcagtt ctcttgcgtt tttgtcgcgc tgctctttgt aggtaatagc gttatcacgg
     4501 taatgattaa cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc
     4561 gcggtgactc tgctcattgc tgcccccaca aacagacttc acgctcaatc tcacgacggg
     4621 tcatcagccc tttccattgc ttaccgccag catatgtcca gcgacgtagc tggtcacatg
     4681 cgcctttgat atcgccctag tttattttgc gaagaagcat cgatgttctg aaattgccag
     4741 cgcccacgtt gtaaacgaac gagtaaagag cgccgcgcgt tgtttccggt atatcgactt
     4801 tgatgtacgg gttaatttgt ctggcgacag tggcaaggtc tttattcagg agggctttgc
     4861 attctgcttc ggtatacgtt ttaccgagca tgatgtcttt tccggtgtgt ccgtggcata
     4921 cagtc
//
LOCUS       DAJTYI010000201         4754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYI010000201 DAJTYI010000000
VERSION     DAJTYI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            278..808
                     /locus_tag="OO564_003820"
     CDS             278..808
                     /locus_tag="OO564_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6654743.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /locus_tag="OO564_003821"
     CDS             815..1048
                     /locus_tag="OO564_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6654744.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="OO564_003822"
     CDS             complement(1628..2746)
                     /locus_tag="OO564_003822"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6654745.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="OO564_003823"
     CDS             complement(2864..2953)
                     /locus_tag="OO564_003823"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654746.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="OO564_003824"
     CDS             complement(3389..3550)
                     /locus_tag="OO564_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6654747.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..4393)
                     /locus_tag="OO564_003825"
     CDS             complement(4202..4393)
                     /locus_tag="OO564_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6654748.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4457..4687
                     /locus_tag="OO564_003826"
     CDS             4457..4687
                     /locus_tag="OO564_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6654749.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
BASE COUNT         1327 a          985 c         1199 g         1243 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatcgctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt tcgttgagct
     4321 tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg gcggtttttt
     4381 cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg taatgatacc
     4441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg tccggttact
     4501 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     4561 ggttgcccgt tccgggaaac tgccgacact ggcaccgccg ttactgcgtc agctggctgc
     4621 catcggggac gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga cgcgggatga
     4681 tagttaagtt tcatgcccgg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg
     4741 gaagggagcg taac
//
LOCUS       DAJTYI010000202         4592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYI010000202 DAJTYI010000000
VERSION     DAJTYI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..565
                     /locus_tag="OO564_003827"
     CDS             <1..565
                     /locus_tag="OO564_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6654750.1"
                     /translation="NTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQN
                     AMRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAV
                     TQAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVA
                     LTTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(611..2602)
                     /gene="tkt"
                     /locus_tag="OO564_003828"
     CDS             complement(611..2602)
                     /gene="tkt"
                     /locus_tag="OO564_003828"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6654751.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            2880..3638
                     /gene="loiP"
                     /locus_tag="OO564_003829"
                     /gene_synonym="yggG"
     CDS             2880..3638
                     /gene="loiP"
                     /locus_tag="OO564_003829"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6654752.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(3844..>4592)
                     /gene="speB"
                     /locus_tag="OO564_003830"
     CDS             complement(3844..>4592)
                     /gene="speB"
                     /locus_tag="OO564_003830"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6654753.1"
                     /translation="IRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1087 a         1243 c         1249 g         1013 t
ORIGIN      
        1 caacactatt gaagcattaa aagatcgtct aattgtccgt cagttgccaa ctcagctgga
       61 gaacattttc ggccaatata ccgccatcag cgccgtacag gatcgtacaa aattggttca
      121 ggatctgcaa aatgccatgc gcaaagccgt tgttgggcca gtggtaattg acggggtcca
      181 gattgaaaat attgattttt ctgacgctta tgaaaagtcg attgaaaacc gaatgaaagc
      241 agaagtcgcc attgccacgc gtaagcaaaa tctggaaaca gagaaaatcc aggcgcaaat
      301 agcggtaacc caggcccagg cagaagcaga cagtaaactg gcggcagaca aagcagaagc
      361 ggaaactatt cgcgtaagag gcgcggcaga ggctgaaact attcgtttaa aaagtgcggc
      421 tgaagcagaa gcaatccgcc tgcgcggtga agctctgcgg gataatcccg gacttgtagc
      481 cctcaccact gccgaacgtt gggacggtaa actgcccgaa accatgatcc ccggcagcac
      541 agttccgttt atctctacta agtaaaaaaa gggccgcttt cgcggccctt ctcacaaaac
      601 gtcttactga ttacagcagc gcttttgctt tcgcaacaac gttatcaaca gtgaagccga
      661 actcttcaaa cagcagctct gccggagcag attcaccgaa ggtggtcatg ccgacgatag
      721 cgccgttcag gccaacatac ttgtaccagt agtcagcaat acccgcttct accgcaacgc
      781 gtgcagtaac cgctttcggc agtacggatt cacggtaagc cgcatcctgc ttgtcaaatg
      841 cgtcggtaga cggcatggac accacgcgcg ctttcacgcc ttcggcagtc agtttttcgt
      901 aggcagcaac cgccagttca acttcagaac cggtggcgat gaagatcagt tccggctgac
      961 cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt tgccagctgc tcttcagtac
     1021 gttcctgctg cgccaggttc tgacgggaga ggataagcgc agtcgggccg tcctgacgct
     1081 caacgccgta tttccacgcg accgcggatt caacctggtc acacggacgc catgtagaca
     1141 tgttcggggt cacgcgcaga gaagcgacct gctctaccgg ctggtgagtc gggccatctt
     1201 cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg gcgctgtttc atcagcgcag
     1261 ccatacgtac ggcgttacgt gcgtattcca cgaacatcag gaaggtggag gtgtacggca
     1321 ggaagccacc gtgcagggag ataccgttag caatcgcggt cataccgaac tcgcgaacac
     1381 cgtagtggat gtagttacct gcagcatctt cgttgattgc tttagaacca gaccacaggg
     1441 tcaggttaga cggtgccagg tcagcagagc cgccgaggaa ttcaggcaac agcgggccga
     1501 acgcttcgat agcattctgc gacgctttac ggctggcgat tttcgccgga ttagcctgca
     1561 gtttagcgat aaactctttc gctttggcgt cgaagtcaga cggcatttcg cctttcatac
     1621 ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc gtaagccgca aacttctcat
     1681 tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc ccactgagca tagatttcag
     1741 acgggatttc gaacggtgca tatttccagc ccagctgttc gcgggtcagg gcaatttcag
     1801 cgtcgcccag cggcgcaccg tgggagtcgt gagtaccggc tttgttcggg gaaccgaaac
     1861 cgatgatggt tttgcacatc agcagggacg gtttgtcagt cactgcgcgc gcttcttcaa
     1921 ctgcgcgttt gatggatgcc gcgtcatgac cgtcgatgtc gcgaataacg tgccagccgt
     1981 aagcttcgaa acgcattgct gtgtcgtcgg tgaaccagcc ttcaacgtga ccatcgatag
     2041 aaataccgtt gtcatcgtag aacgcaatca gtttacccag cttcagcgta cccgccagag
     2101 agcaaacttc gtgggagatg ccttccatca tgcagccgtc gcccatgaag gcgtaggtgt
     2161 agtggtcgac aatgtcgtgg cccggacggt taaactgcgc cgccagcgtt ttttctgcaa
     2221 tcgccatacc gactgcgttg gcaataccct gacccagcgg accggtggtg gtttccacac
     2281 cagcggtata acctacttcc gggtggcccg gagttttaga gtgcagctga cggaagtttt
     2341 tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag caggctgtag atcagcatgg
     2401 agccgtggcc gttggacagc acgaagcggt cacggtcagc ccaggacgga ttctgcgggt
     2461 tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc agccataccc ataggggcac
     2521 ccgggtgacc ggatttggct ttctgtactg cgtccatgct cagcgcacga atagcattgg
     2581 caagctcttt acgtgaggac attttgactc cagatcggat gatgaagggc acgcccttaa
     2641 cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt tgccaacaat ttaccgcaag
     2701 ccgcgcgtca tgtacatgga acatcctttt gccgcttcag aaatctctgg atcatgctcg
     2761 catgttgcgc aatctgctcg cccgtccgct gcgcttttcc ttatactgag actgagcgtc
     2821 gattcacctg caaacggcgc atttttagaa taatcctgac cttgtgcgga agagaaaaca
     2881 tgaaaattcg cgccttattg gtagcaatga gcgtggcaac ggtactgacc ggttgccaga
     2941 atatggactc caacggactg ctctcatcag gagcggaagc ttttcaggct tacagtttga
     3001 gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca ggagatggac agcaaggcga
     3061 cgattgcgcc agccaatagc gaatacgcta aacgtctgac aactattgcc aatgcgctag
     3121 gcaacaatat caacggtcag ccggtgaatt acaaagtgta tatggcgaag gatgtgaacg
     3181 cctttgcaat ggctaacggc tgtatccgcg tctatagcgg gctgatggat atgatgacag
     3241 ataacgaagt cgaagcggtg atcggtcacg agatggggca cgtggcgtta ggccatgtga
     3301 agaaagggat gcaggtggcg cttggtacaa atgccgtgcg agtagctgcg gcctctgcgg
     3361 gcgggattgt cggcagttta tctcaatcac aacttggtga tctgggcgag aaattagtca
     3421 attcgcaatt ctcccagcgc caggaagcag aagccgatga ttattcttac gatcttctgc
     3481 accaacgcgg catcagcccg gcaggtcttg ccaccagctt tgaaaaactg gcgaagctgg
     3541 aagaaggccg tcaaagctca atgtttgacg accatcctgc atccgccgaa cgtgcccagc
     3601 atattcgcga tcgcatcagc gcggatggga ttaagtaaag cctggtggtg tcgaacgtag
     3661 gtcagataag gcgttcacgc cgcatctgac attaatggca catttgttac cttgtgcgca
     3721 ttgccggatg cgatgctggc gcatcttatc cggcctacgg gtgccgaacg taggtcggat
     3781 aaggcgttca cgccgcatcc gacattaatg gcacgtttta cccgtgcgca tcgcatctgg
     3841 tgcttactcg ccctttttcg ccgcctgaat atacagcatc tccagcgcca gcgtcgccgc
     3901 agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta cgtccatccc
     3961 aacgatgttg agatctttca ggccgcgtac cagtttaatg gcgcgatcgg aggtcaggcc
     4021 gccaatcact ggcgtaccgg ttcccggcgc gaaagcagga tccaggcagt cgatatcaaa
     4081 agtcaggtaa accggcatat cacccacaat ctgtttcacc tgggcgataa cgtcatccac
     4141 gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt tatcaaactc
     4201 ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt tcggcgcggt
     4261 atagaacata gtgccgtggt caaattcaca accgttcgca taggtatcgg tgtgggcgtc
     4321 aaagtgtacc agcgccattt tgccgaaatg cttcgcatga gcacgcagca gcggcagcgt
     4381 aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca gcttctcggc
     4441 gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca aaggcatata ccagatcgcc
     4501 gcagtccacg acgttcagac gctcacgcat gtcgaaattc cacgggaagc ggttgtgttc
     4561 ccaggccaga ttcgtcgaaa cctgacggat cg
//
LOCUS       DAJTYI010000203         4464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYI010000203 DAJTYI010000000
VERSION     DAJTYI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            324..1976
                     /locus_tag="OO564_003831"
     CDS             324..1976
                     /locus_tag="OO564_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6654754.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(1987..2157)
                     /locus_tag="OO564_003832"
                     /pseudo
     CDS             complement(1987..2157)
                     /locus_tag="OO564_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2290..2409)
                     /locus_tag="OO564_003833"
                     /pseudo
     CDS             complement(2290..2409)
                     /locus_tag="OO564_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2483..2917)
                     /locus_tag="OO564_003834"
     CDS             complement(2483..2917)
                     /locus_tag="OO564_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6654755.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3094..4403)
                     /gene="ltrA"
                     /locus_tag="OO564_003835"
                     /pseudo
     CDS             complement(3094..4403)
                     /gene="ltrA"
                     /locus_tag="OO564_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1181 a         1083 c         1028 g         1172 t
ORIGIN      
        1 tctccaggaa acttggggcg gttcacgtca gtatcagacc atattaattg ctgaaactga
       61 taaacatcaa accttataga agggtgaatg ctgaaagggg gctatggtga tcgacgtgat
      121 gacaacgaca ggtaagaccg tgacctgata aacctgaatt gtgtcctaga cataaagtcc
      181 gtgaagggaa taaggctcag gtcagcgaat cttatagtgg cccgcagctc tgactgcaat
      241 aaggccggat acagagcagc aactactcgt cgatgtcaga acaacggaag ccttgcaaac
      301 ggggatgcgt tcatacaggt acaatgatac cgttaaaaaa aaacattacg ctaataatgt
      361 tcaccctgtc attactgaca ggaaatcctg ctatagcgta tgaaacagat aaagtatata
      421 aaataaccgt tctgcataca aatgaccacc atggacactt ttggcgtaac aatcatggtg
      481 agtacgggtt atcctcccaa aaaactctgg tggacaatat ccggcagaaa gtaataaata
      541 atggcggaag tgttctatta ttatctggtg gtgatattaa caccggtgta cctgaatcag
      601 accttcagaa ggctgaaccc gatattcgtg gaatgaatct aattggttat gatgcaatgg
      661 ccgtcggtaa tcatgaattt gataatcctc taaatatatt acgtcagcag gaaaaatggg
      721 ccacatttcc tttcctttct gctaacatat atcaaaaaag tacagggagg cgtttattca
      781 gtccatggaa aattttcatc cgtcagaatt tgaaaattgc agtcattggc ttgacgacag
      841 atgatactgc gaaaacagga aattctgaat attttactga tattgagttc cgtcaacctg
      901 ctgctgaggc gaggagtgtc attgatgaac ttaatcagca ggaaaagcca gatattatta
      961 ttgcggcaac acatatgggt cactatgaca atggcgagtc aggttcgaat gctcctggtg
     1021 atgttgaaat ggctcgcagt ttgccgactg gctcattagc aatgattgtg ggaggacatt
     1081 cacaagctcc tgtctgcatg gccagcgata ataaaaaaca gtggaattat attccgggga
     1141 caacctgtgt accagataaa caaaatggca tctggattgt acaagcgcat gagtggggaa
     1201 aatatgtcgg tcaggctgat tttgaattct gtaacggtac aatgaaactg gttaattatc
     1261 agttacaccc agttaatctc aaaatgagaa ttaccagaga agatggaaaa acggaatttt
     1321 ctttttacac ccccgaaatt actgaagatc cacagatgtt atccttactg acaccattcc
     1381 agaataaagg caaagcacaa ctggatgtga aggttggggt tgtgaacgga cgtctggaag
     1441 gggacagaag taaagttcgt tttgttcaga ccagtatggg ccatctgatt ctgagcgctc
     1501 tgacagaacg tattgacgca gattttgctg ttgtcagtgg tggggaaatt cgagattcca
     1561 tagagagtgg taacatcaca tacaaagata tactgaaagt tcagccattt ggtaatacgg
     1621 tagtaagtat tgatttgacg gggaaagaag tagctgacta cctagctaca gtggcacaga
     1681 tgaaaccgga ttccggagcc tacccacaat ttttgaatac cagctttgta gtcaaaaaag
     1741 gaaaaatcga aatgctaaaa ataaaaggta aatcggttga ccttaacaaa aaatatcgta
     1801 tgacaacatt tagctttaat gctactggtg gggatggcta tccacgtatt gataatagac
     1861 ccggatacat aaataccgga tttattgatg ctgaagtact tatcgaatat atccggaaac
     1921 attcaccgct ggatgctgct tcatatgaac caaaaggtga agtcagttgg cagtgataac
     1981 gtaattgacg atcatcacgt gttttttgct gacagtcgct gtgactttgt gaaagtagtc
     2041 tctgcggatg tttgcgatat gggaatgcag ttgctgtatt ttgtgtttct gcttctgcca
     2101 gttgttgctg aatttaacct tgcggcttaa ctgatgttgg agcgctgcca gcttcttcgt
     2161 caatttcgct gattaaagtt tattcattat ggcatgatct gcgtaagcgg ctcgtcagaa
     2221 ccatattgat atttactaag agctcagatc aactttccag ggcaacagat cgcgtacccg
     2281 gtttgccggt cagtcctgga tatgctcaat gacgtaacgc agccattttt ctggctccac
     2341 attgttcaga cggcatgtgc cgatcagcga gtacaacacc gccgcatgtt cgccaccgct
     2401 atcggaaccc cgccggagag agtttcactc tccggctgct cacggaaccg tacgtgacac
     2461 tctcgcgtca tacggctctt gatcactcat tcgatgcacg tagtaaatac cagtgggcaa
     2521 atagcttcgg atactgtttc cttaccagcg ccagctttcg ctgagccttc cggttcgaaa
     2581 gtctgtattt agactgcatc cacttaagca aacgtgactg cattgcactc cacagtcgat
     2641 agctgaagtt tctggaccag aactttccat agtactcaat ccagcctctc actatcgcat
     2701 tgtagcgcct cgcaaaatcc agcaaactct cagctgttga gcgatgtata cgccacttct
     2761 tgattgtttc tgttattttg cacattgctg catttgatgc acccggcata cattttctgt
     2821 acaattcgcc tttgaaattc ttcagagtac gcactttgaa gtcatatccg agaaaggaga
     2881 aactcgtagc gacgtttctc cttttaaacg tgtcaatgta ggcgatattc gtcttccctg
     2941 cgttcaggac caacccgact tccgagaagc gcaccgataa cctgttcttc agccttgttg
     3001 catcactcat tcgtgaacaa tgtactacaa gtcgagtcac ccgagggaac ctccccctct
     3061 ggtgctcacg gaaccggacg tgaacctctc agctcatccg gctcttctca ttgaggcctg
     3121 tccccatagt aacggccagt gtgcaaataa cctcgtttca ctccgcgcaa cacgggccag
     3181 ccagtggctg gcccgtcgtc ggtggcccct aagtcgcttg tgtttacggg gcagccataa
     3241 cacgagtttg cgatcgattt gcctcagcgt cagatacagt gctgatttat aaaaagcacc
     3301 gtagtacttt atccagcctt tgattttcgc attgaacata tgcgccagct cgtacaagga
     3361 cttgtcactg cgattctcct cacctcctga cggattgctt ttcctgctct ggcactcatc
     3421 gccggactga agttaacgaa ggtcttaccc cagcgattca tcgacagacg cggccggaat
     3481 gtatagccca gaaagttgaa gctcgtttcc gggtagtccc cccgtcggtc agcatcctta
     3541 cagtaaacta ttcgtgtttt ttgcggatgc agctgcagcc cgcattgcgc aagccgctgc
     3601 ctcagtccac ttatcagagc gtcagcctgc cactggctgt gacagtggca tacaacatca
     3661 tcggcgtacc tttcgaaggg aacgccggga aactgacgtt gcatccacat atcaaaggca
     3721 tagtgcagga acagatttga gagcaggggg gagattacgc cgccctgcgg cgttcctcga
     3781 ccccgtgcca gcattgctcc atccggcatt tgcaccggtg ccttcagcct gcgctcaatg
     3841 tacatcacca cccagcgtgc ctgagtatga tgctgcactg tcttcagtag caggtcatga
     3901 tcgatatcat caaagaagcc tttaatatcg acttccacca cccagtcaaa cttccagcag
     3961 cgtttcctcg cctgcgcaat cgcctggtgt gcggatttac ccggtcgata accatacgag
     4021 tctgcatgga acaggggttc cagtaccggc tcgagtatct gcttaacgac catctgggcg
     4081 atccggtccg acacggccgg aataccgagc ggacgtacac caccgtcagc tttgggaatg
     4141 tccacacgcc ttaccgcctg cggcatataa ctgccggaag ccattctgtt ccacagtttg
     4201 tagagattgt ctgtaacatt ctcgtcaaac ccggccagtg tctgcccgtc aatgcctgcc
     4261 gccccacggt tggcttttac tcgtttataa gcttcccata cctgtcgttt acttacagga
     4321 aatggctttg ccgattttac agcagtcctc ccatcggcat tgtcgcccgt ggttgccgcc
     4381 gcaccatcag cacaatgggg caagcccttt gctccggacc cattacggtc cttcaccgct
     4441 actacggttt actccgtccc tgta
//
LOCUS       DAJTYI010000204         4422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYI010000204 DAJTYI010000000
VERSION     DAJTYI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1713)
                     /gene="recQ"
                     /locus_tag="OO564_003836"
     CDS             complement(<1..1713)
                     /gene="recQ"
                     /locus_tag="OO564_003836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6654756.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMP"
     gene            complement(1846..2715)
                     /gene="pldA"
                     /locus_tag="OO564_003837"
     CDS             complement(1846..2715)
                     /gene="pldA"
                     /locus_tag="OO564_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6654757.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2880..3347
                     /locus_tag="OO564_003838"
     CDS             2880..3347
                     /locus_tag="OO564_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6654758.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3399..4307
                     /gene="rarD"
                     /locus_tag="OO564_003839"
     CDS             3399..4307
                     /gene="rarD"
                     /locus_tag="OO564_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6654759.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
BASE COUNT         1036 a         1145 c         1127 g         1114 t
ORIGIN      
        1 cggcatctgt tcagccatct caatcaaggt tgcgtcgtta aacaccacgt acggcgggac
       61 attactttca tcggcaatcg atttacgcag tttgcgtaat ttggcgaaca gtttgcgatc
      121 atagttgccg ccgaacgatt tctgcatcgc tttcggtttg agcgccacga tacgcggcac
      181 ggcaagttgc aaagaggatt cgccgcgcag caccgggcgc gcggcctctg tcagttgtag
      241 ggcagaatgc tgggcaatat tttgcgtcac caggccgaga tgaatcagct ggcggatcac
      301 gctcacccaa tgttcatggc ttttatcacg gcccatacca tagactttca gtttgtcatg
      361 accatagtcg cggatacgct ggttattagc accacgaatc acttccacca cataacccat
      421 cccaaaccgc tgattcacac gaccaatggt ggaaagggca atctgagcat cggttgaacc
      481 gtcgtactgt ttcggcggat cgaggcagat atcgcagttc ccgcacggct cctgacgccc
      541 ttcgccaaaa tagttcagca ataccagacg acggcaagtt tgcgcttcgg caaacgcgcc
      601 catcgcattg agtttgtggc gctcgatatc ctgcaactgc ccctgcggct tctcttccag
      661 acaacggcgc agccacgcca tatcagccgg atcgtaaaac agcatcgctt ccgcaggcag
      721 gccatcacgc ccggcgcgac cggtttcctg ataataggat tcgatattgc gcggaatatc
      781 aaagtggacc acgaagcgaa cgtttggttt attgatgccc atgccgaacg ccaccgtcgc
      841 caccacaatt tgcagatcat cgcgctggaa tttctcctgc acgtcggcgc ggacattatt
      901 ttccagcccg gcatgatagg ccgccgcgct aatacccttg ctttgcaggc gcgcagcggt
      961 gtcttctact ttcgcccggc tgttgcagta gataatgcca gacttaccgc gctgttcctg
     1021 cacgtagcgc atcaactgat cgagcggttt gaatttctcc atcagcatgt agcgaatatt
     1081 cggacggtca aaactgctga tttgaatcag cggatcgttc agccccagca ggcgcacgat
     1141 atcctggcgc gtggtgtcgt cggctgtggc ggtcagcgcc ataaacggca gcgtcgggaa
     1201 ccgctggcgc aactgaccga gcgcggcata ttccgggcgg aaatcgtggc cccattggga
     1261 gatacagtgc gcttcatcga cggctaataa caccggattc cagtgcgcca gatgctcaag
     1321 aaagttatcc agcatcaggc gttccggggc gatatacagt aagcgaattt gcccggtgcg
     1381 gcagcctgtc atcacttcaa gttgctgttc gcgggtttgc gtcgagttaa ggcacgccgc
     1441 cgccacgccg ttggcctgta gttgatccac ctgatctttc atcaacgaaa tcagcggtga
     1501 aacaaccacg gtaaggccgt ttagcaataa ggcaggaatt tgatagcaaa gggattttcc
     1561 gccaccagtg ggcataacaa ccaggcaatc gcgaccggaa agcacagtgt cgataatttc
     1621 ttcctggccg gggcgaaact gttggtagcc aaaggtttct tgtaaaacct gtttagctcc
     1681 ggactccaga ttcaacactt ccgcctgcgc cacattcacc ccgattgcct gaaataaaaa
     1741 caggcgctat tttcagcgcc tgcgcgagaa actgcaatgc ttttagtctc tgagagggta
     1801 atcctggatg ctggattcca gatttgccaa ccactcaacc gttcatcaaa acaagtcgtt
     1861 tagcataacc cctacaccga cacgggtctg gttgaagtta tagtcgatga gcgattctcc
     1921 atagccgctg taaacctgag tataaaggcg cacatgtttg gtgatcgggt aacttaagcc
     1981 taactccgcg ccgccgtagc cggtgttcca gttgtactgt cctttcgcac tgagcaccgc
     2041 atcaccgagg tgatagccga ttttaagctg gtagtaaccc atatatttgg tgatatccgg
     2101 gttatcgtca gtattaccca ccacatacca cggcttcact tctaccagcc agttaccgtt
     2161 ttctgccatc aggcgagtat aaaggcggtt ccagctgcgg gaggtcgggt ctgaacgccc
     2221 gttagagtcg tggttatacc ccatctccac atcgcgcagc gtccaacctg caaaacggta
     2281 atcggtggca aaaccgagga acaattgcgg ttcgtagttg gtttcacgaa acggtgaaga
     2341 ctcttcgcta ttggacagtt gccaccagga tttttgcgta taagacgcac ccaacaccga
     2401 gtttgggcct aaaatcccac gccacagcgg aaatgccagg ctcaactgaa actttacttc
     2461 atccttacgc gcattttccg cccagtcgta actggcaatc gcttctttat tcagatcgct
     2521 ggtttgggtg tagatgaggt agttggtgtc ataaggatag agcgtgaacg gattgtcatg
     2581 ctcctgcagc atattggcga taatactgcc acgcaccgct ggcgcgtcat gcacctcttt
     2641 caccgttgcc tcttgtgcat atactgccat aggcaacata aacaccggca acaaccagcc
     2701 ctgcagagtc cgcatcggtc gtgttctccg taacgaatta ttgataaggt gaatgttttt
     2761 ctgcgggcta ttttacatac ttaactccat tgccgttagt tatcccttcc taaagtagaa
     2821 accaacaaca aagaagcata aaatcaacat ttcattaacc aatgaagtgt aaggatgata
     2881 tgtctgccgt actgaccgct gaacaagccc tgaaattagt gggtgagatg tttgtttatc
     2941 acatgccatt taaccgcgca ttggggatgg aactggagcg ttacgaaaaa gagttcgcac
     3001 agctggcctt taaaaatcag ccgatgatgg tgggtaactg ggcgcaaagc attttgcacg
     3061 gcggagtcat tgcgtcggcg ctggatgtcg ccgccggtct ggtgtgcgtg ggaagtacct
     3121 taacccgcca cgaaaccatc agtgaagatg aactacgcca gcggctatcg cggatgggga
     3181 ccattgatct tcgcgttgat tatctgcgcc caggcagagg cgagcgtttt actgctacca
     3241 gtagcctgtt gcgtgcaggc aataaagtcg ccgtcgcccg cgttgaatta cacaatgaag
     3301 aacagcttta tattgccagt gccaccgcca cctatatggt aggttgaaga cataaaagca
     3361 ggtaaactgc tgttaccaat taaaaatgag tattcccgat ggatgcaaaa caaacgcggc
     3421 agggcgtatt actcgctctt gccgcttatt ttatttgggg tattgcgcca gcatacttca
     3481 agctgattta ctacgtgccc gccgatgaga tcctgacgca tcgcgtgatc tggtcgtttt
     3541 tctttatggt ggtgctgatg agcatttgcc gccagtggtc ctatttaaaa acgctgattc
     3601 agacgccaca gaaaattttt atgctggcag tctctgccgt gctgattggt ggcaactggc
     3661 tactgtttat ctgggcggtg aacaatcacc atatgctgga agcgagcctt ggttacttta
     3721 ttaacccgct ggtgaacatt gtgctgggga tgattttcct cggcgagcga ttccgccgga
     3781 tgcaatggct ggcggtgatt ctggcgatat gtggcgtgtt ggtccagctg tggacttttg
     3841 gttcgctacc tattatcgcg ctggggctgg catttagttt tgccttctac ggcctggtgc
     3901 gcaagaagat tgcggtagaa gcacaaaccg gcatgttaat tgaaaccatg tggctgctgc
     3961 ccgtggcggc gatttacctg tttgctattg ccgacagttc aaccagccat atggggcaaa
     4021 acccgatgtc gctgaattta ctgctgatcg ccgccggtat tgtcactacc gtaccgctgt
     4081 tgtgttttac tgccgctgcc acgcgcctgc gtctctcaac gttaggcttt tttcagtaca
     4141 ttggcccgac gctgatgttc ctgctggctg tgacgtttta tggtgaaaaa ccgggtgccg
     4201 ataagatggt gactttcacc tttatttggg tggcgctggc aatttttgtg atggacgcga
     4261 tttatacgca gcgtagaacg tccaaatcaa aaataaatat attctgaaaa aatatgccga
     4321 ctcttgtatg agagtgggca tattgccatg ctgacaggag actcacacaa ggatgtaaaa
     4381 tttatgttac gtatgtttat cccaacatct aacggtaaga tt
//
LOCUS       DAJTYI010000205         4293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYI010000205 DAJTYI010000000
VERSION     DAJTYI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..88)
                     /locus_tag="OO564_003840"
     CDS             complement(<1..88)
                     /locus_tag="OO564_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6654760.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKS"
     gene            complement(76..543)
                     /locus_tag="OO564_003841"
     CDS             complement(76..543)
                     /locus_tag="OO564_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6654761.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(540..1016)
                     /locus_tag="OO564_003842"
     CDS             complement(540..1016)
                     /locus_tag="OO564_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6654762.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(1000..1323)
                     /locus_tag="OO564_003843"
     CDS             complement(1000..1323)
                     /locus_tag="OO564_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6654763.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            1548..1766
                     /locus_tag="OO564_003844"
     CDS             1548..1766
                     /locus_tag="OO564_003844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654764.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(2035..2658)
                     /locus_tag="OO564_003845"
     CDS             complement(2035..2658)
                     /locus_tag="OO564_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6654765.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(2655..2843)
                     /locus_tag="OO564_003846"
     CDS             complement(2655..2843)
                     /locus_tag="OO564_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6654766.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(2840..3202)
                     /locus_tag="OO564_003847"
     CDS             complement(2840..3202)
                     /locus_tag="OO564_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6654767.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(3199..3489)
                     /locus_tag="OO564_003848"
     CDS             complement(3199..3489)
                     /locus_tag="OO564_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6654768.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(3489..4211)
                     /locus_tag="OO564_003849"
     CDS             complement(3489..4211)
                     /locus_tag="OO564_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCR6654769.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(4204..>4293)
                     /locus_tag="OO564_003850"
     CDS             complement(4204..>4293)
                     /locus_tag="OO564_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR6654770.1"
                     /translation="VLEVHVCEHCCAELMSDPNSSMYEEEDDE"
BASE COUNT          964 a         1117 c         1004 g         1208 t
ORIGIN      
        1 tggatttact ccagaagtga tcacccccct gtctacagag ccagatgtga aggatgatga
       61 gtaaaattat cgctatcatc gaaggcattg cgtcctgatg tattcctgaa gcgttctcag
      121 tgctgtttgg tcgcggataa ttccgtcccg gataccgaga acgtttcgtc cagcaactgg
      181 agagagttcg acggtggcat cattgcccat gccggaggcg ctggaggttt cggctgagga
      241 tggcacaggg cattttcctt tgacgagcac cctgccacca ttatcaagct tgcgccgaag
      301 agcatcattt tcagctttcg catcagctaa ctccttcgtg tatttagcat cgagtgcatc
      361 agcagcacgc tggcgttgct gcatgtcagt aatggtggcg gtcgcctgct tcagctcact
      421 gactttttta tctcgctgct ctttgtaggt catggcgtta tcacggtaat gattaaccgc
      481 ccatgacagg cagacgatga tgcagataac cagagcggag ataatcgcgg tgactctgct
      541 catacatcaa tctctctgac cgttccgcct gcttctttga attttgcaat caggctgtca
      601 gccttatgct cgaactgacc ataaccagcg cccggaagtg aagcccagat attgctgcaa
      661 cggtcaattg cctgacgaat atcaccgcga tcaatcatcg gtaaagcgcc acgttcttta
      721 atctgttgca gtgccacagc gtcctggctt ttcggagaga agtctttcag gccaagctgc
      781 ttacggtaag catcccacca acgggaaaga agctgataac gtccggcggc tgttgatttt
      841 agttttgggt ttagtgtgac aagtttgcga ggatgatcgg agtaatcagt aaatagctct
      901 ccgcctacaa tgacgtcata accatgattt ctggttttct gacgtccgtt atcagttccc
      961 tctgaccacg ccagcatatc gaggaacgcc ttacgttgat tattgatttc caccatcttc
     1021 tactccggct tttttagcag cgaagcgttt gataagcgaa ccaatcgagt cagtaccgat
     1081 gtagccgatg aacacgctcg ttatataagc gagattgcta cttagtccgg cgaagtcgag
     1141 aaggtcacga atgaaccagg cgatgatggc gcacatcgtt gcgtcgatta ctgtttttgt
     1201 aaacgcaccg ccattatatc tgccgcgaag gtacgccatt gcaaacgcaa ggattgcccc
     1261 gatgccttgt tcctttgccg cgagaatggc ggctaacagg tcatgttttt ctggcatctt
     1321 catgtcttac ccccaataag gggatttgct ctatttaatt aggaataagg tcgattactg
     1381 atagaacaaa tccaggctac tgtgtttagt aatcagattt gttcgtgacc gatatgcacg
     1441 ggcaaaacgg cattaggttg ttagcgcaac ctcatgccgc ccactttcac gaagatcatg
     1501 tgtagaaggc cgcagcgtaa ctatcactga tgaattcagg atagtcagtg gctacggctc
     1561 agtttggatt gtggcgaccg gtgctgatct ccggtttgct gcaactgcct acagctggct
     1621 acgtggccac accgaatcca gcgaaagatt cttgccctta cacatcagcc tgtgcattca
     1681 ccacaacgga aagagcactg gctaaccagg ctcgccgact cttcacgatt atcggctcaa
     1741 tgctcttacc tgttgtgcag atataaaaaa tcccgaaacc gttatgcagg ctctaactat
     1801 tacctgcgaa ctgtttcggg attgcatttt gcagacctct cagcctgcga tggttggagt
     1861 tccagacgat acgtcgaagt gaccaactag gcggaatcgg tagtaagcgc cgcctctttt
     1921 catctcacta ccacaacgag cgaattaacc catcgttggg tcaaatttac ccaactttat
     1981 tcaataagtc aatatcatgc cgttaatatg ttgccatccg tggcaatcat gctgctaacg
     2041 tgtgaccgca ttcaaaatat tgtctgcgat tgactcttcc ttgtggcatt gcaccaccag
     2101 agcgtcatac agcggcttaa cagtgcgtga ccatgtgggt tgggtaaggt tggggattag
     2161 catcgttaca gcgcgatatg cggcgcttgc tggcatcctt gaatagccga cgcctttgca
     2221 tcttccacac tctttctcaa caactctccc ccacagctct gttttggcaa tatcaaccgc
     2281 acggcctgta ccgtggcaat ctctgcatct tgcgcccggc gtcgcggcac tacggcaata
     2341 atccgcataa gcgaatgttg cgagcacttg cagtaccttt gccttagtat ttccttcaag
     2401 ctttgcaacg ccacggtatt tccccgatac cttgtgtgca aattgcatca gatagttgat
     2461 agccttttgt ttgtcgttct ggctgagttc gtgcttacca cagaatgcag ccattccgaa
     2521 tccggcttgt gattgcgcca tccccatagc agccatcaca tcagtaccgg aaagagagtc
     2581 agaagccgtg gcccgtggtg agtcgctcat catcgggctt tttggcgaat gaaatttagc
     2641 tacgctttcg agtctcatcg tcttcccctc ttgccctgtt tgaccatcag gacgccgtta
     2701 actattacgt gacgctcgcc tttgctgtct cggttgtact tgagcactgt tcctcttgcg
     2761 caggaaagca tcctcgccac ttcggtctga ttgcctcgtg tctggataag aagctctggt
     2821 atcgtttgaa ttgtggcgtt catacgttct ccagttcggt gatttttatt ccaagccttc
     2881 cgcctggtac tttcacacca cgaattacgc gaatgtcatc gaattgctcg tcgtcttccg
     2941 caaatccggc gtggataagg gagtcgagta aacctttcag gatgttatcg aggtcgcggc
     3001 ggcgggagtc tggaacgtct gcgatgactt tgatgcggag tcgtgatttg gtgaaaatgt
     3061 ctaacttgag ttggaggatt atttgctgaa cttcttttcg gtatttctgg cctttatcgc
     3121 tgatgtagta ttggtttccc cgtcttcgcc agtaggtgtt caccgacggc gggtatggaa
     3181 gcacaaactg atattcgctc atgacttaat cttcccctcc ttcagcagta tcgcctgcgt
     3241 cctgatcacg ccttcgaggt ggtaaagtct ggcgtctttg ttgtcgagat tatgggtgcg
     3301 tcggtcgatt tcatcgtgac acgcgctaca agcccatgcg ccgatcaggt cgtcaggctt
     3361 cattcccgtt ccgcaaattc cagccatccg gtaatgtgcc agaactgtag tttcaggatt
     3421 accattgcat acgccgtaaa tacgtacctg gcattctctg ccgcgtgctt ctttgcgtag
     3481 attagccatt aagcagcctc ccctgttact ttcagcattc cattatcgag cagctttctg
     3541 gtcagccact gttgaccacg cccggtgatt tttgtggtga acgatatctg tattccgtga
     3601 tttgtgttga ccgctgtttc tttcactgtg aaatagccgc gatccatata ttcctgcatt
     3661 ggcacattgc gccgggaacc tgaagcaata aggattttgt gatcgcgcat ccacgcaaac
     3721 agtttgtttg gaccaattcc aacaaccttt gcaaagtttc caatcaaaat tccgctggcc
     3781 tcgccaacgc gatcggcaaa ctcaacttta ggtgcggcaa ttgcgagctg gttttccagt
     3841 tgcattttct gctcagcaag atcagcagca aggcgcaacg cttccggtag cgttttgggg
     3901 atattaaccg cagcttcttc aagctctcgc cagcggtcaa caaggcgagc ggtgaattcc
     3961 ggcgacaact gggctacaac gacaatactg tctcgctttc cttgttcgcc ttcgaacaca
     4021 tacacacaaa aactttgatt taagcctaac ccattgattc ttccacaatc ctcaatttga
     4081 ggaagccgga taacaccatt tttagccagc gtttcgatgg tacgtttcac attgtcatga
     4141 cgcttaccaa ccaactcagc gatttcaatg cttgtcattt tgatggcatt gccatttatt
     4201 aactcattca tcgtcttctt cctcgtacat tgagctattc ggatcgctca tcagttctgc
     4261 gcagcagtgc tcacacacgt gaacttccag cac
//
LOCUS       DAJTYI010000206         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYI010000206 DAJTYI010000000
VERSION     DAJTYI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO564_003851"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO564_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6654771.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO564_003852"
     tRNA            complement(324..399)
                     /locus_tag="OO564_003852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO564_003853"
     tRNA            complement(406..480)
                     /locus_tag="OO564_003853"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO564_003854"
     tRNA            complement(597..681)
                     /locus_tag="OO564_003854"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO564_003855"
     tRNA            complement(690..765)
                     /locus_tag="OO564_003855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO564_003856"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO564_003856"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6654772.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO564_003857"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO564_003857"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6654773.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO564_003858"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO564_003858"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6654774.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1038 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtacgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggat
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJTYI010000207         4028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYI010000207 DAJTYI010000000
VERSION     DAJTYI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..213)
                     /locus_tag="OO564_003859"
     CDS             complement(<1..213)
                     /locus_tag="OO564_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6654775.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO564_003860"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO564_003860"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6654776.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO564_003861"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO564_003861"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6654777.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO564_003862"
     CDS             2894..3634
                     /locus_tag="OO564_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6654778.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..>4028
                     /gene="ruvC"
                     /locus_tag="OO564_003863"
     CDS             3669..>4028
                     /gene="ruvC"
                     /locus_tag="OO564_003863"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6654779.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKS"
BASE COUNT          995 a          993 c         1134 g          906 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagc
//
LOCUS       DAJTYI010000208         4009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYI010000208 DAJTYI010000000
VERSION     DAJTYI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..305)
                     /locus_tag="OO564_003864"
     CDS             complement(<1..305)
                     /locus_tag="OO564_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6654780.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
     gene            complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OO564_003865"
     CDS             complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OO564_003865"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6654781.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2318..3967
                     /locus_tag="OO564_003866"
     CDS             2318..3967
                     /locus_tag="OO564_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6654782.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1039 a         1006 c          936 g         1028 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      541 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      601 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      661 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      721 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      781 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
      841 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
      901 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
      961 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1021 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1081 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1141 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1201 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1261 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1321 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1381 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1441 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1501 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1561 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1621 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1681 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1741 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1801 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     1861 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     1921 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     1981 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2041 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2101 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2161 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2221 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2281 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2341 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acctgtatga
     2401 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2461 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2521 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2581 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2641 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2701 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2761 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     2821 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     2881 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     2941 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3001 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3061 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3121 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3181 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3241 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3301 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3361 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3421 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3481 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3541 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3601 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3661 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3721 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3781 aatcgcgcgc agttggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     3841 cgagaaatac catattgccg atggtgtttc ccgcgaaggc ggcggtggcg agtatccgtt
     3901 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga
     3961 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaat
//
LOCUS       DAJTYI010000209         3970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYI010000209 DAJTYI010000000
VERSION     DAJTYI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..227)
                     /locus_tag="OO564_003867"
     CDS             complement(<1..227)
                     /locus_tag="OO564_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6654783.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQ"
     gene            complement(439..786)
                     /gene="pspE"
                     /locus_tag="OO564_003868"
     CDS             complement(439..786)
                     /gene="pspE"
                     /locus_tag="OO564_003868"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6654784.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(829..1050)
                     /gene="pspD"
                     /locus_tag="OO564_003869"
     CDS             complement(829..1050)
                     /gene="pspD"
                     /locus_tag="OO564_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6654785.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1059..1418)
                     /gene="pspC"
                     /locus_tag="OO564_003870"
     CDS             complement(1059..1418)
                     /gene="pspC"
                     /locus_tag="OO564_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6654786.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1418..1642)
                     /gene="pspB"
                     /locus_tag="OO564_003871"
     CDS             complement(1418..1642)
                     /gene="pspB"
                     /locus_tag="OO564_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6654787.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1696..2364)
                     /gene="pspA"
                     /locus_tag="OO564_003872"
     CDS             complement(1696..2364)
                     /gene="pspA"
                     /locus_tag="OO564_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6654788.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2531..3508
                     /gene="pspF"
                     /locus_tag="OO564_003873"
     CDS             2531..3508
                     /gene="pspF"
                     /locus_tag="OO564_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6654789.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3628..>3970)
                     /locus_tag="OO564_003874"
     CDS             complement(3628..>3970)
                     /locus_tag="OO564_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6654790.1"
                     /translation="AVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGS
                     MIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDA
                     AMKILQDALSD"
BASE COUNT          955 a         1005 c          954 g         1056 t
ORIGIN      
        1 cattgatggt agaactgatt aaatatgggt agtggtttta aatcattact gtgaaattga
       61 tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg tcgctgtgta
      121 attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt tgcggtaaat
      181 gttccaccgt agatttcgtc caggtaatcc gtaattttct gtttcatgtt gcctttccgt
      241 ggtagcggtc ccatgaggcg agagactact ccgcagaaaa taaactgtca attgagcaca
      301 agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt tgtgagctga
      361 cgcaaataat ctatagggat gtaaataagg tgtgacagaa aaaaaacggc gcataagcgc
      421 cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc
      481 ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgcct gcccggactg
      541 gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaactg cggtggcaat
      601 gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata
      661 ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac
      721 cagtgccaga gcaagtaagc ctttttttaa acatgtaagc gtccttctat ggatgaaaat
      781 tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc accttttata
      841 acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca cagccagcaa
      901 cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac ccgccgggcc
      961 atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccaa gcttcacctt
     1021 ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg gaaacggcta
     1081 cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg cgtttcactt
     1141 gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag ctgctcacca
     1201 aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta agcaaccagg
     1261 gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac cagttttacc
     1321 ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc ctgctgtgga
     1381 atacgccata attttttatt gagattaatg cccgccatta tcgatccctc cagttcggat
     1441 gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt tttgcttcat
     1501 caaccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga ccagaacgat
     1561 tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg gttaacggaa
     1621 tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc gcgttcggac
     1681 gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt gtgccagttg
     1741 ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg atttttgttt
     1801 accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt cgaaagattc
     1861 gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca catcgcgcga
     1921 cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg tttcgctcaa
     1981 tttgttttcc agctcgccaa tctctttctt catgcgtgcc agcgtatcgt ccaccagcgt
     2041 cacttcatgt tccagggact taatcagatc ggtcagtttc tgtttttcaa ttaacgctgc
     2101 acgtgccaga tcctctctct ctttcagcag cgccagttcg gctttttcct gccattcaac
     2161 ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg ccaacgcacg
     2221 cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca tcagacgcac
     2281 cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat tcacgatgtc
     2341 ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt caggattgtc
     2401 ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt tataaagatc
     2461 tgattgaaga atcaacagca acatgccagg atgaattagc gaattacact aacaagtggc
     2521 gaatttcatc atggccgaat acaaagataa tttacttggt gaggcgaaca gctttctcga
     2581 agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca tcatcggcga
     2641 acgcggcacc ggtaaagagt tgattgccag ccgcctgcat tatctctcct cccgttggca
     2701 agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg attccgaact
     2761 gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg ggagatttga
     2821 acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga tgatggtgca
     2881 ggaaaaatta ttgcgtgtga ttgagtacgg tgaactggag cgcgttggcg gtagccagcc
     2941 gttgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg cgatggtcaa
     3001 tgaaggtact tttcgcgctg acctgctcga ccggctggct ttcgatgtag tgcaactgcc
     3061 gcccctgcgc gagcgcgaaa gcgacattat gctgatggca gaacactttg ccattcagat
     3121 gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca gagaaacatt
     3181 gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg aacgttcagt
     3241 gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc cctttaaacg
     3301 gcgtccacct gaagaagcca tcgccgtttc agaaaatacc tcgcttccga cgctgccgct
     3361 ggatttacgc gagtttcaga tgcagcagga aaaagagttg ctgcaactca gtttgcaaca
     3421 ggggaaatat aaccagaagc gcgcggctga attactgggg ttaacttatc atcagttccg
     3481 cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg ccggatgcgg
     3541 cgtgaatgct ttattcggca tacataatca atacgtcgtt tgcaggccta ataagcgcag
     3601 cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca aaattttcat
     3661 tgccgcatcg aattgcgcat ccgggatggt cagcggatac aggaagcgaa tcacgttgcc
     3721 gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct ggattttctg
     3781 tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg caatcatcga
     3841 ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa tcaacgtgtt
     3901 tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt cgataatatt
     3961 gagcaccgcg
//
LOCUS       DAJTYI010000210         3855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYI010000210 DAJTYI010000000
VERSION     DAJTYI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..223
                     /locus_tag="OO564_003875"
     CDS             <1..223
                     /locus_tag="OO564_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6654791.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            269..478
                     /gene="hha"
                     /locus_tag="OO564_003876"
     CDS             269..478
                     /gene="hha"
                     /locus_tag="OO564_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6654792.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            517..1107
                     /locus_tag="OO564_003877"
     CDS             517..1107
                     /locus_tag="OO564_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6654793.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1347..1607
                     /gene="repA"
                     /locus_tag="OO564_003878"
     CDS             1347..1607
                     /gene="repA"
                     /locus_tag="OO564_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6654794.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1831..1905
                     /gene="tap"
                     /locus_tag="OO564_003879"
     CDS             1831..1905
                     /gene="tap"
                     /locus_tag="OO564_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6654795.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1898..2755
                     /gene="repA"
                     /locus_tag="OO564_003880"
     CDS             1898..2755
                     /gene="repA"
                     /locus_tag="OO564_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6654796.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3455..3646
                     /locus_tag="OO564_003881"
     CDS             3455..3646
                     /locus_tag="OO564_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654797.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1047 a          937 c          965 g          906 t
ORIGIN      
        1 ttttcagcga gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa
       61 tattaatcag tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga
      121 tcccgtgcta cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga
      181 tgctgatccg gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat
      241 cattcatctg ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca
      301 gttacggcga tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag
      361 tttatcaaac agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt
      421 aatcaccgga aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata
      481 attcagcaat aattaattgc caggagcaga taaataatga agttgattat ttttattctc
      541 attgttctga ttattgcaac gctacttatc cggattatcc tcagaagtgt aaatcagcat
      601 tctcctttgc tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata
      661 ctgtccagcg gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt
      721 tttatgaaag gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta
      781 cgggtcgcgg atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg
      841 cagttattca ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg
      901 ttcagtattg ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga
      961 cgccgggata ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc
     1021 cacgatgcca gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat
     1081 cagcagtcac cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga
     1141 aacgggaaaa agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt
     1201 tcacagttta aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat
     1261 cttcgccaca attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat
     1321 ccctgtttga gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat
     1381 tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg
     1441 ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc
     1501 tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg
     1561 agaaaatcat caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg
     1621 tggcgtagca tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag
     1681 gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata
     1741 atcttcttta actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca
     1801 acaatcaagc tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg
     1861 tgctcgctcc ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta
     1921 ttaccgccag gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct
     1981 tccgttctgc gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc
     2041 cattcatgtg gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg
     2101 tcgccgggcc attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa
     2161 ccgcgtccag tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc
     2221 tgccggaaaa ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg
     2281 actgattacc taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat
     2341 cacgttcaca tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc
     2401 gcgccgcagt cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct
     2461 gggtatggat gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta
     2521 ccagacagag cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag
     2581 gcagcgtcag gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg
     2641 gcgcttcact gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg
     2701 catgattctg tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg
     2761 acctcctcag aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg
     2821 cacaaaaaat cagcgctaca tacaaaaaac aacctcatca tccaccttct ggtgcatccg
     2881 gtcccccctg tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat
     2941 taaactgcaa gggacttcac cgtaaggtta caaccgttca cgtcataaag cgccagccgt
     3001 caaccctaca gggtgcaatg tatcttttaa acacctgtgt atatctcctt taaactactt
     3061 aattacattc atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacagatatg
     3121 cacctcccac cgcaagcggc gggccccaac cgaagccggt ttagttacaa catgcaaaca
     3181 taaaccccta aaaaacccaa gtccaacgca gaactgaaac cacaacgccc ctcctcataa
     3241 ctgaaaagcg ccgccgcccc gcccgaaggg cgggaacaga gtcgctttta attatgaatg
     3301 ttgtaactac atcatcatcg ctgtcagtct tctggctgga cgtatcgagt acacgctcgt
     3361 aagcggccct cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt
     3421 cgggaccact ccgaccgcgc acagaagctc tatcatgact gaaaacgggt atggcttagc
     3481 agggctgggg atgggtaagg tgaaatctgt caatcagtac cggctgacgc cgggcttcgg
     3541 cggttttact ccggtatcac atgtaaccac agcgtgccgc cttccatgcc gctggcgcgg
     3601 catcagaata atacaagcag catttaatgc atttgcaaaa gtgtgagtaa tattaagtgg
     3661 tcgtttcagg gatatcgtta agacttgagc gataggaatt aaaaccccaa aaagattaaa
     3721 aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga acggaaccga
     3781 cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt tgctccgttt
     3841 ccctcaaaat ggacg
//
LOCUS       DAJTYI010000211         3774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYI010000211 DAJTYI010000000
VERSION     DAJTYI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            33..>3774
                     /gene="hrpA"
                     /locus_tag="OO564_003882"
     CDS             33..>3774
                     /gene="hrpA"
                     /locus_tag="OO564_003882"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6654798.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQW"
BASE COUNT          929 a          966 c         1067 g          812 t
ORIGIN      
        1 attaatcagg cagactactg cccactaacg ttatgacaga acaacaaaaa ttgaccttta
       61 ctgccttgca acagcggctg gattcgctga tgctgcgcga cagactgagt ttttctcgcc
      121 gtctgcattg tgttaagaag gttaaaaatc ctgatgcaca acaggccatt ttccaggaga
      181 tggcgaaaga gattgaccag gcggcaggga aagtcctgct gcgtgaagcg gcacgaccgg
      241 aaatcactta tcctgacaat ttaccggtta gtcagaaaaa acaggacata ctcgaagcga
      301 ttcgtgatca ccaggtggtg atcgtcgccg gggaaaccgg ctccggtaaa acgactcagc
      361 taccgaaaat ctgtatggag ctggggcgcg gtattaaagg gctgatcggt catacccagc
      421 cgcgtcgcct ggcggcacga acggtggcca accgtattgc ggaagagctg aaaacggagc
      481 cgggcggttg catcggttac aaagtgcgtt tcagcgatca cgtaagtgat aacacgatgg
      541 tcaagctgat gaccgacggg atcctgctgg cggagatcca gcaagaccgc ctgctgatgc
      601 agtacgacac catcattatt gacgaagcgc acgaacgcag cctgaatatc gattttctgc
      661 tcggctattt gaaagagttg ctgccgcggc gtcctgacct aaaaatcatt atcacttccg
      721 cgactatcga cccggaacgc ttttcgcgcc actttaataa tgcgccgatt attgaagtct
      781 ccggtcggac ctatccggtg gaagtgcgct atcgcccgat tgttgaagaa gccgatgaca
      841 ccgagcgcga tcagttgcag gcgatttttg acgccgtaga cgaactaagc caggaaagcc
      901 ccggcgacat tctgatcttt atgagcggcg agcgggaaat tcgcgatacc gccgatgcgc
      961 tgaacaagct gaacttacgc cataccgaaa tcttgccgct ttatgcgcgg ctttcgaaca
     1021 gcgaacagaa ccgcgtgttc cagtcgcaca gcggacggtg cattgtgctg gcgaccaacg
     1081 tcgcggaaac ctcgctgacc gtaccgggga ttaaatacgt catcgacccc ggtacagcgc
     1141 gtatcagccg ctacagctat cgcaccaaag tgcagcgttt gccgattgaa cccatttcgc
     1201 aggcgtctgc caatcagcgt aaaggccgct gtggtcgtgt gtccgaaggg atctgtattc
     1261 gtctctattc cgaagacgat ttcctctcgc gcccggagtt taccgatccg gagattctgc
     1321 gtaccaacct ggcctcggtt attttgcaga tgaccgcgct ggggctgggc gatatcgctg
     1381 cgttcccgtt tgtcgaagca ccggataaac gcaatatcca ggatggcgtg cgtctgctcg
     1441 aagaactggg cgcgatcacc acggatgaac aggccagcgc ctataaactg acgccgctcg
     1501 gtcgccagct ctcgcagttg cctgtcgacc cacgtctggc gcgtatggtg ctggaagcgc
     1561 aaaaacatgg ctgcgtgcgt gaggcgatga ttatcacgtc cgcgctctcc atccaggatc
     1621 cgcgcgaacg tccgatggac aaacagcagg catcggacga aaaacatcgt cgcttccacg
     1681 acaaagaatc cgacttcctc gcgtttgtaa atctgtggaa ttatcttggc gagcagcaaa
     1741 aggcgctttc ttccaacgcc ttccgtcgcc tgtgtcgtac cgattacctc aactatctgc
     1801 gcgtgcgcga atggcaggat atctacaccc agttgcgcca ggtggtgaaa gaacttggca
     1861 ttccggtcaa cagcgaaccg gcggagtatc gtgaaattca catcgcctta ctgaccggtt
     1921 tgctttccca tatcggcatg aaagatgccg ataaacagga atataccggt gcacgtaacg
     1981 cgcgtttctc catcttcccc ggttctggtt tattcaaaaa gccgccgaaa tgggtaatgg
     2041 tggcggaact ggtagaaacc agccgcctgt gggggcgcat tgctgcgcgt atcgacccgg
     2101 aatgggtaga acccgttgct cagcatttga ttaaacgcac ctacagcgaa ccgcactggg
     2161 aacgggcgca gggcgcggtg atggcaacgg aaaaagtcac tgtttatggt ttgccgattg
     2221 ttgctgcgcg caaggtcaac tacagccaga tcgattcggc gttatgtcgt gaactcttta
     2281 ttcgccacgc tctggtggaa ggtgactggc agacgcgtca cgcattcttc cgtgaaaacc
     2341 tgaaactgcg ggccgaagtg gaagagctgg aacacaaatc acgtcgccgc gatattctgg
     2401 ttgatgacga aacgctgttt gagttctacg accagcgcat cagccacgat gtaatctccg
     2461 ctcgtcactt cgatagctgg tggaaaaaag tcagccgcga aacgcctgat ttgctcaact
     2521 ttgaaaagag catgttgatc aaagaagggg cggaaaaaat cagcaagctg gattacccga
     2581 acttctggca tcagggcaat ctcaagctgc gtttgagcta tcagtttgag ccaggcgcgg
     2641 acgcggacgg tgtgaccgta catattccgc tgccgttact taaccaggtt gaggaaagcg
     2701 ggtttgaatg gcagatcccc ggtctgcgcc gcgaattggt gattgctctg attaaatcgt
     2761 tgccgaaacc ggtacgccgt aattttgtgc ctgcaccaaa ctacgccgaa gcgtttttag
     2821 gccgcgtcac accgctggag ttaccgttgc tcgacagcct tgagcgcgag ttacggcgga
     2881 tgaccggcgt taccgttgac cgcgaagact ggcactggga tcaggtgccc gatcacctga
     2941 aaatcacctt ccgcgtggtg gatgacaaaa acaagaagct aaaagaaggg cgctcgctac
     3001 aagatctgaa agatgcgctg aaaggcaaag tgcaggaaac gctgtccgcg gtggcggatg
     3061 acggtatcga gcagagcggc ttacatatct ggagttttgg tcagctgccg gaaagttacg
     3121 aacagaagcg tggcaactat aaagtgaagg cgtggccggc gctggtggat gaacgcgaca
     3181 gcgtggcgat caaactgttt gataacccgc tggagcaaaa acaggcaatg tggaatggtc
     3241 ttcgccgtct actgctgcta aatatttcct cgccgatcaa gtatttacat gagaagttac
     3301 caaacaaagc caagctgggg ctgtacttta acccgtatgg caaagtgctg gagctgatcg
     3361 acgactgtat ctcctgcggt gtggatcaac tgatcgacgc caatgatggc ccggtctgga
     3421 cggaagaagg ctttgctgcg ctgcatgaaa aagtgcgcgc cgaactgaat gacacggtgg
     3481 tggatattgc gaagcaggtc gagcaaatcc tcactgcggt gttcaatatc aacaaacgtc
     3541 tgaaagggcg ggtggatatg accatggcac tggggctttc tgacattaaa gcgcagatgg
     3601 gcgggttggt atatcgcggt tttgtcactg gtaacggctt caaacgtctg ggcgacacgc
     3661 tgcgatattt gcaggcgatt gaaaaacgac tggaaaaact ggcggttgat ccacatcgcg
     3721 atcgtgcaca gatgctgaaa gtcgaaaacg tccagcaggc gtggcagcaa tgga
//
LOCUS       DAJTYI010000212         3749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYI010000212 DAJTYI010000000
VERSION     DAJTYI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..880
                     /gene="adrA"
                     /locus_tag="OO564_003883"
                     /gene_synonym="dgcC"
     CDS             <1..880
                     /gene="adrA"
                     /locus_tag="OO564_003883"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6654799.1"
                     /translation="WPHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVGVMGVNVLPS
                     TAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWWLSLPII
                     VVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFDNCRRHN
                     RDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIGRFGGDEFAVIM
                     SGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYREWLKSADLA
                     LYKAKKAGRNRTEVAA"
     gene            complement(897..1706)
                     /gene="proC"
                     /locus_tag="OO564_003884"
     CDS             complement(897..1706)
                     /gene="proC"
                     /locus_tag="OO564_003884"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6654800.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1826..2284
                     /locus_tag="OO564_003885"
     CDS             1826..2284
                     /locus_tag="OO564_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6654801.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2467..2991
                     /gene="aroL"
                     /locus_tag="OO564_003886"
     CDS             2467..2991
                     /gene="aroL"
                     /locus_tag="OO564_003886"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6654802.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3041..3232
                     /gene="yaiA"
                     /locus_tag="OO564_003887"
     CDS             3041..3232
                     /gene="yaiA"
                     /locus_tag="OO564_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6654803.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3490..>3749
                     /locus_tag="OO564_003888"
     CDS             3490..>3749
                     /locus_tag="OO564_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6654804.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVL"
BASE COUNT          920 a          899 c         1012 g          918 t
ORIGIN      
        1 ctggccgcat ttagcctggc agatagcgag cagggccgtc gatccgctta gccgggaaat
       61 ttacaactta aaaaccgatg cagtattagc gggaatgtgg gtaggcgtaa tgggcgtaaa
      121 cgtgctgcct tccaccgcta tgttgatgat tctgtgtctg aatttgatgg gggcaggcgg
      181 cccccgtctg tttgtcgcgg gtctggtgtt gatggtggtt tcctgccttg tcaccctcga
      241 gcttacgggc attaccgtgt cgttcaatag tgcgccgctg gaatggtggc tctcccttcc
      301 cattattgtc gtttatcctc tgctgtttgg ctgggtcagc taccagacgg caaccaaact
      361 ggcggaacat aaacgcaggt tgcaggtcat gagtactcgc gacggcatga cgggcgtgta
      421 taaccgacgt cattgggaaa ctatgttacg caatgaattt gataactgtc ggcggcataa
      481 tcgcgatgca acgttactga ttatcgatat cgaccatttc aagagcatca acgatacctg
      541 gggtcatgat gtgggcgatg aagcgattgt ggcgcttacc cgacagttac aaattaccct
      601 gcgcggtagc gatgtgattg gtcggtttgg cggcgatgag tttgcagtaa tcatgtccgg
      661 tacgccagct gagagcgcca ttaccgccat gttacgggtg cgtgaagggc taaacacgtt
      721 acgcttgccg aatacgccac aggtaacttt acggattagt gtgggggttg cgccgctgaa
      781 cccacaaatg agtcactatc gtgagtggtt gaaatcggca gatttggcgc tttacaaagc
      841 aaagaaagcc ggacgtaacc gcaccgaagt ggcggcctga cgtccggcga aagtcatcag
      901 gatttgctga gtttttctga tttttccata cacttcgtca tcgcttcgat cactgcggca
      961 cggaagcctt tctcttccag tacgcgtacc gcttcaatgg tggtacctcc cggtgagcag
     1021 accatatctt tcagtgcccc cggatgttct cccgtttcca gcaccatttt tgcggaaccc
     1081 attaccgcct gagcggcaaa tttatacgcc tggacgcgtg gcatcccgcc cagcacggcg
     1141 gcgtcggcca tcgcttcgat aaacataaat acgtaggctg gcgaagaacc gctcacaccg
     1201 accaccgggt ggatcatcgg ctcagcaatt acttccgctt cgccaaagca gcggaaaata
     1261 ttcagcacat cagcggtatc ttctggggtt accagcgcgt ttggcgttac ggaggtcatc
     1321 ccggcattaa ccagcgcggg agtgttcggc atggcgcgga taattttccg gtcatggccc
     1381 agcgcgcggg caagctggtc gagcgtgaca cctgcagcaa tagaaacgac cagagagtct
     1441 ttattcaggc tggaggtgat ttcgctaagc actttaatca tgatgccagg tttaacggca
     1501 gcaaaaatga tgtcggcgat ttgcgccact tcttgcgccg attctgcggc gttgatgccg
     1561 aactggtcat gcagggcggc gactttatcc ggggaggggg tgtataccca gatttgccct
     1621 ggaagcacct gaccgctggc aattagaccg ccgagaatgg cttttcccat attgccgcag
     1681 ccaataaaac cgattttctt ttccattgcc tcactcctgc cgtgaaattc attgttttga
     1741 taatcgctgg cagaagcata aacagaacta tgccggaagg caaaagcgcg acacaataga
     1801 ggattaccca acaaaggatg actttatgac aatttgggtg gatgccgacg cgtgtcccaa
     1861 tgtaattaaa gagattttgt atcgcgcggc ggaacgtatg cagatgccgc tggtactggt
     1921 agcaaaccag agtttacgcg tgccgccatc gcgatttatt cgtacgctgc gcgtcgcggc
     1981 aggtttcgac gttgccgata acgaaattgt ccggcagtgt gaagcgggcg atttggtgat
     2041 caccgcagat atacctttgg ctgctgaagc catcgagaaa ggcgcagcgg cgcttaatcc
     2101 gcgcggcgaa cgttacacgc cagcgaccat tcgtgagcgc ctgacgatgc gcgattttat
     2161 ggatacctta cgtgccagtg ggatccagac cggcggacca gatagccttt cacaacgtga
     2221 ccgccaggcc tttgccgcgg agctggagaa gtggtggctg gaagtgcaac gtagtcgtgg
     2281 ctaaatgtaa tttattattt acacttcatt cttgaatatt tattggtata gtaagggatg
     2341 tattgagatt ttcactttaa gtggaatttt ttctttacaa tcgaaattgt actagtttga
     2401 tggtatgatc gctattctca tgacaccggc tttcgccgca ttgcgaccta ttggggaaaa
     2461 cccacgatga cacaacctct ttttctgatc gggcctcggg gctgtggtaa aacaacggtc
     2521 ggaatggccc ttgccgattc gcttaaccgt cggtttgtcg ataccgatca gtggttgcaa
     2581 tcacagctca atatgacggt cgcggagatc gtcgaaaggg aagagtgggc gggatttcgc
     2641 gccagagaaa cggcggcgct ggaagcggta actgcgccat ccaccgttat cgctacaggc
     2701 ggcggcatta ttctgacgga atttaatcgt cacttcatgc aaaataacgg gatcgtggtt
     2761 tatttgtgtg cgccagtatc agtcctggtt aaccgactgc aagctgcacc ggaagaagat
     2821 ttacggccaa ccttaacggg aaaaccgctg agcgaagaag ttcaggaagt gctggaagaa
     2881 cgcgatgcgc tatatcgcga agttgcgcat attatcatcg acgcaacaaa cgaacccaac
     2941 caggtgattt ctgaaattcg tagcgccctg gcacagacga tcaattgttg attttagagc
     3001 gcctatactt aacgttcatc ccgtgaaata aggaagaacg atgccaacga aaccgcctta
     3061 tcctcgtgaa gcatatatag tgacgattga aaaaggaaag ccaggacaga cggtaacctg
     3121 gtaccaactc agagccgatc atcctaaacc agactcgttg atcagtgaac atccgacggc
     3181 tcaggaagcg atggatgcga aaaaacgcta tgaggaccct gacaaagagt gaccgcatca
     3241 gactgctcgg aagggattct gagtgccact acaagggatc tgcgtcacat ttttcataat
     3301 tcatgttttt ctaataatta gaatattaaa caataacatt ccattactgg aatcatttgg
     3361 aatctttaca ttatgccgtg cacgtctgct gctacgcttt ttgtcatttg tagcacaagt
     3421 aagtgtcagc agtggtgctt cacacttgcc cggtaattaa cgacgaaaga aaagtaaggt
     3481 ggatgaacaa tgagtgcgtc gttggcgatc ctgaccatcg gcattgtacc tatgcaggag
     3541 gttttgccgc tcctgacgga atacattgac gaagataata tttcccatca tagcctgctg
     3601 gggaagttaa gtcgtgaaga agtgatggcg gagtacgcgc cagaagcagg cgaagacacc
     3661 attctcacat tattaaatga caaccagctg gcccatgttt cgcgtcgtaa agtggagcga
     3721 gatctgcaag gtgtggttga agtgctcga
//
LOCUS       DAJTYI010000213         3703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYI010000213 DAJTYI010000000
VERSION     DAJTYI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(64..822)
                     /locus_tag="OO564_003889"
     CDS             complement(64..822)
                     /locus_tag="OO564_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6654805.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO564_003890"
     CDS             complement(830..1933)
                     /locus_tag="OO564_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6654806.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="OO564_003891"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="OO564_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..>3703)
                     /locus_tag="OO564_003892"
     CDS             complement(3181..>3703)
                     /locus_tag="OO564_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6654807.1"
                     /translation="NMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNP
                     AEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPA
                     TPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNL
                     WNNGGIQYAPPVR"
BASE COUNT         1042 a          972 c          865 g          824 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttg
//
LOCUS       DAJTYI010000214         3689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYI010000214 DAJTYI010000000
VERSION     DAJTYI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..656
                     /locus_tag="OO564_003893"
     CDS             <1..656
                     /locus_tag="OO564_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6654808.1"
                     /translation="LEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVA
                     DALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNP
                     QQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPCACCGKPADDPHHLIGH
                     GQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            669..1025
                     /locus_tag="OO564_003894"
     CDS             669..1025
                     /locus_tag="OO564_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6654809.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            1040..1861
                     /locus_tag="OO564_003895"
     CDS             1040..1861
                     /locus_tag="OO564_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6654810.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2005..2080
                     /locus_tag="OO564_003896"
     tRNA            2005..2080
                     /locus_tag="OO564_003896"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2038..2040,aa:Ile,seq:cat)
     gene            2182..2258
                     /locus_tag="OO564_003897"
     tRNA            2182..2258
                     /locus_tag="OO564_003897"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2216..2218,aa:Arg,seq:tct)
     gene            2758..2889
                     /locus_tag="OO564_003898"
     CDS             2758..2889
                     /locus_tag="OO564_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6654811.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(3170..3505)
                     /gene="iraM"
                     /locus_tag="OO564_003899"
     CDS             complement(3170..3505)
                     /gene="iraM"
                     /locus_tag="OO564_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6654812.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          948 a          735 c          986 g         1020 t
ORIGIN      
        1 cactcgagca acttgctcat caaaacctgt cagcatggat gattgacgtc atcggtcacg
       61 caataagcgg tacgcaggag cgtgaattat ctctggctga attatcctgg tgggcggtcc
      121 gcaatcaggt ggcggacgcg ctaccggaag cggtattacg tcgttcgctg gggttgcgtg
      181 cggaaaaaat ccgctcaatg taccgtgaaa gcgacatcgt accgggagag cagaccgcca
      241 ccagcatact gaaacagcgc acaaaaaatc ttgcgccgct gcctcacgcc caccagcaac
      301 agaacccaca acaggaaaag acggtggtca gcattgccgt tgatcctgag tctccggaat
      361 ctttcatgaa acgacctaaa cgtcgcagct gggttaacga gaaatacaaa cgctgggtga
      421 agacacagcc gtgtgcgtgt tgtggtaagc cagccgacga tccccatcac ctgattggtc
      481 atggtcaggg cggaatgggg acaaaatctc acgatatttt cacgctaccg ctgtgtcggg
      541 agcatcacaa cgagcttcat gcggatcctc tggcgttcga agaaaagcat ggttctcagg
      601 ttgatttaat ttttcgtttt cttgatcacg cctttgcaac tggcgtgctt gggtaaaaga
      661 ggtgactgat gctcatagat ttggttttac cttacccgcc gacggtgaac acttactggc
      721 gacgccgtgg cagcacatat tttatctcgg aggagggaaa gcgttatcgc cgggctgtgg
      781 cgcttattgt tcgccagcag cggctgaaat taagcctgtc cggaaggctg gcgataaagg
      841 tgattgcaga gccaccggat aagcgtcgtc gcgacctgga caatatcctg aaagcaccgc
      901 tggatgcgct gacgcatgcg ggagtgttaa tggacgatga gcagtttgat gaaatcaata
      961 tcgttcgtgg tcagccagta tctggtggac gtctgggggt gaagatttac cccataatgc
     1021 attaagagca ggtcaaaaaa tgaaactgga agatttaccg aaatactact ccccaaaatc
     1081 ccctggcctg accgatgcat cggcctcaac gtcaaaagat gcgctgagta tcactgatgt
     1141 gatggccgcg cagggcatga cacagaatcg ggctgagatg ggtttttctg cgttcctggg
     1201 gaaaatgggc atcagtatga atgacagggc gcgggcaaca gaattactgg cagattatgc
     1261 actcagtcgg tgcgatcgtg tggcggcgtt gagaaaactt ccggcagaaa taaaaccggt
     1321 agtgatgcgc attatggctt cgtacgcttt tgaggattat gcccgcagcg cagcgagtaa
     1381 aaagcagtgc ccttgttgct atggggaaaa atttattgaa agcatagttt ttacaaacaa
     1441 gatccagtat ccggatggta agccgccggt atgggcaaag tgtacgaaag gtgtgtatcc
     1501 gtcttactgg gaagaatgga aaaaagtcag ggaggtggta aaagttgcct gtccggagtg
     1561 tggcggaaag ggtgaggttt ccaccgcctg taaggattgc cgtgggcgtg gtgtcgccat
     1621 tcatcgtgaa gagtcggtaa aacgtggtat gcctgttatc agagactgcc agcgttgtgg
     1681 tggtcgtggc tatgaaagac taccatcaac ggaggcattt aatgctatat gcgaggtgac
     1741 aaaccagata acacgcgcgt catgggaaaa aacagttaag aaattttatg atgcgctggt
     1801 gacccggttt gatattgaag aagcatgggc tgagcggcag ttaaaaaagg taactaggta
     1861 acaaggttga tttttccgga atctgtggta aattcgtcat aatgatgggc gttttatgcc
     1921 tgacgttaga agagtttcta caacccgccg ctgagcgggt tttttattgc gaaattaatt
     1981 acggaccgtt attattctgc tcccggccct ttagctcagt ggtgagagcg agcgactcat
     2041 aatcgccagg tcgctggttc aaatccagca agggccacca tcacaaaccg ccattagctt
     2101 atcaggaaga gcagacgaca cgataacagg gttgttggtg cggggggcgg gtccccgatg
     2161 gcggtccatt atcggtattc agcgttgtta gctcagccgg acagagcaat tgccttctaa
     2221 gcaatcggtc actggttcga atccagtaca gcgcgccata ttcattcttc cagattcctt
     2281 ccggcagagc cttatactga aatatacctg gctcaggata ttgttgaaaa tattatatat
     2341 ttgtcaaaaa taaaagttct gttaagtatt gattgagtat ttgttatacg gtctaatggt
     2401 tttttcagca ttaagtattt atcattcata tggtgtgggt agagtgaata ttgatgaggc
     2461 gtcggggtgt ttcatcctta ggcagcgtat tgatatagtc aatgcagcac gagcaaaggc
     2521 cttcagccgt ttgacagttt tgttctgtac tcctgatcgt ctttcgggaa gagacgttat
     2581 tattctgaat agtgatgcta tacagagggt ttgcgatgag ttcatggttg ctaattcaga
     2641 attatttgct cttgttcagg agtacaacag aatagccagg acctgtggta tggatgaact
     2701 tcggattact catctggggt agatacatat ctggattatc acctgttacg gtaaaaagtg
     2761 attgcttact gtttttgtga atggcattgc agcagccgga taatgtcagt gccggctgac
     2821 ggtgtgctgg tggcgggtgt ggtggttgtt gctttcccgt tgctgaaaaa gaaaacgcca
     2881 gactgttagc cgggtatcag ttagcgggag aaatttttaa atacttcaca attcaggcgg
     2941 ttgactgttg tctggtttgc ggggagtttg ttaaaagaaa ctggcatggt gaatccccct
     3001 gtgcggaggg gcaatcagcg agtaggtata tgggataatc gcggattcag gtgctggtac
     3061 tgaattcacc gggaggcacc cggcaccatg caatggcaca tagcgccact ctccagcccc
     3121 tctccggagg ggctgtttat attgattttg tcagatgtga gtaaactcct tatggacttt
     3181 gttgttttag tccataagga catatttgca gagtgcaacg gttattaaag cattcattca
     3241 atacgttatc tgtatttgta gggcattcct ggctgttttt gattaaattc cagaatgttt
     3301 tattgaatgg tactacgttg taaatggtta caggtagcac tttgttattg agcatgatgc
     3361 ctgtgtgagt cagtgtaaat atactttcag gaggtaagaa agcatccgat tgataccaga
     3421 ttattaattt tattttactc catatgactg aaaaagatat tccgcatgat ggctggataa
     3481 ctgtatcaat cacaatccac ttcatttagt ttccttgttt atgccttgct ggtgatgttc
     3541 tgaaaagtat aaatgatatt tttgaattaa accatagagc agaattattt ttctgatgtt
     3601 gtttattgtt tatttaaatg cagggtggtt tatatctcgt cttgtagttt atccatgcat
     3661 atctgcttga taatcaggtt tttatttaa
//
LOCUS       DAJTYI010000215         3606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYI010000215 DAJTYI010000000
VERSION     DAJTYI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            20..1057
                     /locus_tag="OO564_003900"
     CDS             20..1057
                     /locus_tag="OO564_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6654813.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1085..1174)
                     /locus_tag="OO564_003901"
     tRNA            complement(1085..1174)
                     /locus_tag="OO564_003901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Ser,seq:cga)
     gene            1268..2065
                     /gene="mtfA"
                     /locus_tag="OO564_003902"
     CDS             1268..2065
                     /gene="mtfA"
                     /locus_tag="OO564_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6654814.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2166..2241
                     /locus_tag="OO564_003903"
     tRNA            2166..2241
                     /locus_tag="OO564_003903"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Asn,seq:gtt)
     gene            2403..>3606
                     /locus_tag="OO564_003904"
     CDS             2403..>3606
                     /locus_tag="OO564_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6654815.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLE"
BASE COUNT          992 a          831 c          879 g          904 t
ORIGIN      
        1 cgaattttgg tgcgatctga tgggaagacg aaggaaaaat cctgaacacg aaaaattacc
       61 tccaaatgta tacccaaata aatatagtta tgtatggaaa ccaacatcca gagaatctgt
      121 aacactaacc gccatcaagg atggtttagc tgctttatgg aaaaagtatg aggaaactgt
      181 aaataatcgc gatcgtgcaa tgacattcgg tcgcttgtgg gaaaaattcc tcgccagcgc
      241 ctattacagt gacctcagtc caagaacaca aaaagattat ctgcaacatc aaaaaaagtt
      301 gcttgccgta ttcggtaagg tgccggcaga ttccataaaa ccagaacaca tccgtcgata
      361 catggacaag agaggggaac agagtaaaac gcaagccaac catgaaaaaa gcagtatgtc
      421 ccgcgtttac agttgggggt atgagcgagg gtacgtgaag gctaacccat gtgcaggtgt
      481 aagtaaattc aaggccaaaa accgcgaacg atatgtaacc gacaaagaat accaggcagt
      541 attaagcgtt gcacctcttc ctgtttttat cgcaatggaa attgcctatc tgtgtgcagc
      601 gagggtttcc gatgtgttat cgctgaaatg ggagcagatt ggaaacgacg ggatctttat
      661 ccagcaaggg aaaacaggaa aaaaacagat aaaagcatgg agtccacgat tacaggcggc
      721 gatcgaaaaa gcaaaacagt taccaacatc cgcctatgta atcagcaatc aatacggcaa
      781 ccgatatatg tacaaaggct ttaacgaaat gtgggtagaa gcaagaaatc gcgcaggcaa
      841 aatttcaggt attttaaccg acttcacctt tcatgatctg aaggcgaaag gaatttcaga
      901 ctatgaagga agcagtcggg ataagcaact tttctctggt cacaaaaccg aggggcaagt
      961 gctaatctat gacaggaagg ttaaagtttc accgacactt gatgtcccgt tacctgaaaa
     1021 tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga
     1081 attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     1141 agaccggtcc gttcagccgc tccggcatct ctccgctcag atggttgcca tgatgccagg
     1201 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     1261 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     1321 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa
     1381 attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
     1441 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     1501 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     1561 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     1621 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     1681 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     1741 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     1801 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     1861 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     1921 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     1981 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     2041 gttttcggcg acgaatgttc attaattaac aactttgcag attaattaac caattgaaat
     2101 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     2161 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     2221 cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga
     2281 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     2341 atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc
     2401 atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag
     2461 tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc
     2521 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     2581 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     2641 acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa
     2701 acgtggcgct ggcgtggcat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     2761 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     2821 ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg
     2881 ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc catctggagt
     2941 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     3001 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     3061 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     3121 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga
     3181 tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc
     3241 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     3301 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt
     3361 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     3421 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     3481 aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata
     3541 ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc
     3601 tggaag
//
LOCUS       DAJTYI010000216         3594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYI010000216 DAJTYI010000000
VERSION     DAJTYI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            40..1020
                     /gene="rluD"
                     /locus_tag="OO564_003905"
     CDS             40..1020
                     /gene="rluD"
                     /locus_tag="OO564_003905"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6654816.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1017..1748
                     /gene="pgeF"
                     /locus_tag="OO564_003906"
     CDS             1017..1748
                     /gene="pgeF"
                     /locus_tag="OO564_003906"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6654817.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            1878..>3594
                     /locus_tag="OO564_003907"
     CDS             1878..>3594
                     /locus_tag="OO564_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6654818.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIG"
BASE COUNT          913 a          875 c          987 g          819 t
ORIGIN      
        1 agccgtaaaa ccccaacgat aaacgaagaa gcagtatata tggcacaacg agtacagctc
       61 actgcaacgg tgtccgaaaa ccaactcggt caacgcttag atcaggcttt ggccgaaatg
      121 ttcccggatt attcacgttc gcgaataaaa gaatggatcc tcgaccagcg cgtgctggta
      181 aacggcaaag tttgtgataa gccgaaagaa aaagtattgg gtggcgagca ggttgccatc
      241 aactctgaga ttgaagaaga agcgcgtttt gaaccgcagg atatccctct ggatatcgtc
      301 tatgaagatg aagatatcat cgtcattaat aaaccgcgcg acctagtggt acatcctggc
      361 gcgggtaacc cggatggcac ggtactgaat gcgttgcttc attactatcc gcccattgcc
      421 gatgtaccac gtgcgggcat cgtccatcgt ctggataaag acaccactgg cctgatggtt
      481 gtggcaaaaa ccgttccggc tcagacgcgt ttagtcgaat ctttgcaacg gcgtgaaatt
      541 actcgtgagt atgaagcggt ggcgattggt catatgaccg ctggcggtac agtagatgaa
      601 cccatcagcc gccacccgac caaacgtact cacatggcgg tgcatccgat gggcaaaccg
      661 gcggtgactc actatcgcat catggaacac ttccgtgtgc acacgcgtct gcggttgcgt
      721 ctggaaactg gacgtacgca ccagatccgc gtgcatatgg cccatatcac tcatccgctg
      781 gtgggcgatc cggtttatgg tggccgtccg cgtccgccaa aaggtgcttc ggaagcattt
      841 atctccacgc tgcgtaagtt tgaccgccag gcgcttcatg caaccatgct gcgtctttat
      901 cacccgatct ccggcatcga aatggaatgg catgcgccta ttccacaaga tatggtggag
      961 ctgattgagg tgatgcgcgc cgatttcgaa gagcataagg atgaagtgga ctggttatga
     1021 gtaagctgat tgtcccgcag tggccgctgc caaaaggtgt tgcggcctgt agctccactc
     1081 gtatcggcgg cgtgagcttg cccccgtatg actcactcaa cctcggtgcc cattgtggcg
     1141 ataacccgga tcacgttgag gagaatcgca agcgactttt tgctgcgggc aatttgcctt
     1201 ctaaaccggt ctggcttgag caggtacacg gcaaagatgt gcttaagctt actggcgaac
     1261 cttatgcctc aaaacgagca gatgcctctt atagcaatac gcctggcacg gtttgcgcag
     1321 tgatgactgc cgactgcctc cctgtgctgt tttgcaatcg agcgggaacg gaagtcgccg
     1381 ccgttcatgc tggctggcgt ggactgtgcg caggcgtgct ggaagagacg gtttcctgtt
     1441 ttgctgataa acctgaaaac attctcgcct ggttagggcc ggcaattggt ccacgcgcgt
     1501 tcgaagtggg ggcggaggtt cgcgaggcgt ttatggcagt agacgctaaa gcaagtgcag
     1561 ctttcattca gcatggtgat aagtatctgg cggatattta tcagcttgcc cggcagcgtc
     1621 tggcgaacgt gggtgttgag caaattttcg gcggcgaccg ttgtacatat acggaaaatg
     1681 agactttctt ctcttatcgt cgcgacaaga ccaccggtcg tatggcaagt ttcatttggc
     1741 tgatataacc taaagaatca agacgatccg gtacgcgtga ttttcttttc acattaatct
     1801 ggtcaataac cttgaataat tgagggatga cctcatttaa tctccagtag caactttgat
     1861 ccgttatggg aggagttatg cgtctggatc gtcttactaa taaattccag cttgctcttg
     1921 ccgatgccca atcacttgca ctcgggcacg acaaccaatt catcgaacca cttcatttaa
     1981 tgagcgccct gctgaatcag gaagggggtt cggttagtcc tttattaaca tccgctggca
     2041 taaatgctgg ccagttgcgc acagatatca atcaggcatt aaatcgttta ccgcaggttg
     2101 aaggtactgg tggtgatgtc cagccatcac aggatctggt gcgcgttctt aatctttgcg
     2161 acaagctggc gcaaaaacgt ggtgataact ttatctcgtc agaactgttc gttctggcgg
     2221 cacttgagtc tcgcggcacg ctggccgaca tcctgaaagc agcaggggcg accaccgcca
     2281 acattactca agcgattgaa caaatgcgtg gaggtgaaag cgtgaacgat caaggtgctg
     2341 aagaccaacg tcaggctttg aaaaaatata ccatcgacct taccgaacga gccgaacagg
     2401 gcaaactcga tccggtgatt ggtcgtgatg aagaaattcg ccgtaccatt caggtgctgc
     2461 aacgtcgtac taaaaataac ccggtactga ttggtgaacc cggcgtcggt aaaactgcca
     2521 tcgttgaagg tctggcgcag cgtattatca acggcgaagt gccggaagga ttgaaaggcc
     2581 gccgcgtact ggcgctggat atgggcgcgc tggtggctgg ggcgaaatat cgcggtgagt
     2641 ttgaagaacg tttaaaaggc gtgcttaacg atcttgccaa acaggaaggc aacgtcatcc
     2701 tgtttatcga cgaattacat accatggtcg gcgcgggtaa agccgatggc gcaatggacg
     2761 ccggaaacat gctgaaaccg gcgctggcgc gtggtgaatt gcactgcgta ggtgccacga
     2821 cgcttgacga atatcgccag tacatcgaaa aagatgctgc gctggaacgt cgtttccaga
     2881 aagtgtttgt tgccgagcct tctgttgaag ataccattgc gattctgcgt ggcctgaaag
     2941 aacgttacga attgcaccac catgtgcaaa ttactgaccc ggcaattgtt gcagcagcga
     3001 cgttgtctca tcgctacatt gctgaccgtc agctgccgga taaagccatc gacctgatcg
     3061 atgaagcagc atccagcatt cgtatgcaga ttgactcaaa accagaagaa ctcgaccgac
     3121 tcgatcgtcg tatcatccag ctcaaactgg aacaacaggc gttaatgaaa gagtctgatg
     3181 aagccagtaa aaaacgcctg gatatgctca acgaagaact gagcgacaaa gaacgtcagt
     3241 actccgagtt agaagaagag tggaaagcag agaaggcatc gctttctggt acgcagacca
     3301 ttaaagcgga actggaacag gcgaaaatcg ctattgaaca ggctcgccgt gtgggggacc
     3361 tggcgcggat gtctgaactg caatacggca aaatcccgga actggaaaag caactggaag
     3421 ccgcaacgca gctcgaaggc aaaactatgc gtctgttgcg taataaagtg accgatgccg
     3481 aaattgctga agtgctggcg cgttggacgg ggattccggt ttctcgcatg atggaaagcg
     3541 agcgcgaaaa actgctgcgt atggagcaag aactgcacca tcgcgtcatt ggtc
//
LOCUS       DAJTYI010000217         3547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYI010000217 DAJTYI010000000
VERSION     DAJTYI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            180..437
                     /locus_tag="OO564_003908"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO564_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO564_003909"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO564_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6654819.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO564_003910"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO564_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6654820.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO564_003911"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO564_003911"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6654821.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO564_003912"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO564_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT          912 a          845 c          850 g          940 t
ORIGIN      
        1 cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacc
//
LOCUS       DAJTYI010000218         3514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYI010000218 DAJTYI010000000
VERSION     DAJTYI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            289..807
                     /locus_tag="OO564_003913"
     CDS             289..807
                     /locus_tag="OO564_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6654822.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="OO564_003914"
     CDS             814..1047
                     /locus_tag="OO564_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6654823.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="OO564_003915"
     CDS             complement(1349..1816)
                     /locus_tag="OO564_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654824.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2886..3077)
                     /locus_tag="OO564_003916"
     CDS             complement(2886..3077)
                     /locus_tag="OO564_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6654825.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="OO564_003917"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="OO564_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DAJTYI010000219         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYI010000219 DAJTYI010000000
VERSION     DAJTYI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..606
                     /locus_tag="OO564_003918"
     CDS             <1..606
                     /locus_tag="OO564_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6654826.1"
                     /translation="RLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAK
                     SIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYD
                     NGKSGEKFLQELCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINH
                     AAMNTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            621..1043
                     /locus_tag="OO564_003919"
     CDS             621..1043
                     /locus_tag="OO564_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6654827.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1101..1457
                     /locus_tag="OO564_003920"
     CDS             1101..1457
                     /locus_tag="OO564_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654828.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            1550..1768
                     /locus_tag="OO564_003921"
     CDS             1550..1768
                     /locus_tag="OO564_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6654829.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            1770..2132
                     /locus_tag="OO564_003922"
                     /pseudo
     CDS             1770..2132
                     /locus_tag="OO564_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2132..2368
                     /locus_tag="OO564_003923"
                     /pseudo
     CDS             2132..2368
                     /locus_tag="OO564_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2561..2797
                     /locus_tag="OO564_003924"
                     /pseudo
     CDS             2561..2797
                     /locus_tag="OO564_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2797..3162
                     /locus_tag="OO564_003925"
     CDS             2797..3162
                     /locus_tag="OO564_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6654830.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          944 a          708 c          967 g          837 t
ORIGIN      
        1 cgcctgaatc gtcagttgcg ggtggaaaaa attctgaacc gctccggcat ccagccgttg
       61 caccggaagt gttcgtttgc gaattaccgg gcgcagaacg acggtcagcg acatgcactg
      121 agtcaggcga aatccatagc tgacgaactg atgacaggct gtacgaattt tgtgttcagc
      181 ggtaagcccg gcaccggaaa aaatcatctt gcagccgcga ttggcaaccg gctgatggcg
      241 aaggggcgta gcgtgattat cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc
      301 tacgacaacg gcaaatccgg tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg
      361 gtcctggatg aaattggcat gcagcgggat acgaaaaacg agcaggtggt actgaaccag
      421 attgttgatc gccggacggc atcgttacgc ggtgtgggga tgctgacaaa tattaaccat
      481 gcagcgatga atacacttct cggcgagcgg gtgatggatc gcatggtcat gaacggcggg
      541 cgctgggtga attttaactg ggagagctgg cgtccgaatg ttagccattc gagggttgtt
      601 aagtagtttc aggaggattt atggcgaaac cttttactcc cgaacagcgg gaagaactga
      661 agacgcgaat tgtggaactc gtgcatcagg acggtcgggt cacgattcgg cagttgtcag
      721 atgaaacagg tatcagtcgt gcgtctgtcg gtcgcttatg catggaactg gtcgcaagtg
      781 gtgatgtata taattctggc tacggggtat ttccctctga acaggctcat aaagactggc
      841 aaaacgcccg caaaaagcta tcgagggtaa aggtgaagaa accggttgtg gttgatcctg
      901 accttatctg gtcattacca gacggagaaa tacgccgcta cgacaggcgc ctgaatataa
      961 tctgtcgcga gtgccggaag agcgaagcta tgcagcgcgt gctggcgttt tatcagggga
     1021 aatttcagga ggcggtactg tgagtaaaat tagctatcag gcttcaatta ccgctggtat
     1081 tcgcatcaaa ggagaggagc atggaaataa aaccagaaga tgagttaagc aatatcgttt
     1141 tatttccggt aaaagaggat gacccacgta atcaggttaa ttttctttat gaaccatcgg
     1201 aaagaccata ttgccatcac gcctctgtcc gggttgacga aaaagagcgt caggtccgct
     1261 gtaaaatctg cggtgcggtt gtggagccgt ttgactggat gctctctgtg gcaaaaagag
     1321 aaaccagact ggcagatgat gtaaggctat tgcgccagga ggaacaggaa aggcggagaa
     1381 atatagaaaa gctgatacag attgagcgta acgcgaaagc gcggatacgc agggcgacaa
     1441 aatccagaac tgaataatta aatttagcac tgttaaaaat ttaatcctta accggaggga
     1501 tttctgcacc ctcaaatcat caggagaccg cccgaaaggg cggtaatgaa tggttacatt
     1561 atttagaaaa aaatatccgc gaaagagtag aacaacagaa ttcctgtttc tcattctgtt
     1621 tatcgtgttg atgacaccga tatccccgct aatttttgtc tggataatcg gaaaaataat
     1681 tgagccagtt attgaattgt ataacgacgt ggtatgggcg tcattcaaca cactgcacaa
     1741 taaaattaat ccgtataagg aaagctgata tggcactgac gaaaaaacaa cgtgcagaac
     1801 tgcgcatgaa gttcggtggt cgctgcgctt attgcggctg cgaacttggc gaaaagtggc
     1861 atgcagacca tgtaaaaccg gtcattcgtt ttgatggaaa tatgcttcac caggaacgtg
     1921 acgatatatc caacatggta ccagaatgcc acccatgcaa tctgcacaag cattgcagta
     1981 gcctagaaga ttatcgacga attatcagtg atggtcgtcg tgaattcctt gcgtccggga
     2041 aaggcaaagc gctggttcgt atgggattgg ttgaaatgaa atctgacccg gttgtgttct
     2101 ggtttgaaaa atatcaagaa ggggctacgg catggccact attaccaaag agcgactgca
     2161 atggctggct aacatttctg gccgcgatga tattgacgat atagacggcg gtgaaattcg
     2221 tgaactggcg cttatcgctc tggcatcact ggaagcagag cctgtagcgg aatgcattgt
     2281 tgaagatggg ggcatgtgta ttgacgggtt cggtgagtat gtgggtcact cgctgcctga
     2341 tggaatgcat gagctttatg ctgccccgcg aatgcgacaa cctgatggat ggaaagccgt
     2401 agctgtagcg tggaaggtga cgtttactca agttgaccag gaatctaata cgttcactgc
     2461 tatatatttt gacaaagcgg aagttgagcg ttgggtacga ctgcataaag catgtgattt
     2521 tcgggcagat ataacaccgc tttacgcagc gccggcagtg ccggttgcag taaacgacga
     2581 catggcttac gcattccatc acgcactgtc agattcatcg cttggctctg gtgaaatcga
     2641 agaaattaaa accggtttgc gtgctgcctt tgccaacgtc actatccaac cagagccgat
     2701 agtgccggat gaaatcgggc caaacgatag taatacgttt gattatgttg atggctggaa
     2761 cgcctgccgc gctgccatgc ttaagggaga taaataatga ttaatcgaac caaactggag
     2821 cacattctcg aatatgccag gcagcagaaa tgtattgggc aactttgtaa aattccacca
     2881 ggagatatgg ttgaaatcgt ggaaatggcc atgcgtaagg ctggcaactc tccggtaact
     2941 ctggctggct ggataagctg tagtgatgca gttcctgctg aatattgcga tgtgattctt
     3001 ctcgatgatc tcgggaatgt attccccggt tcctgggata aggttttttg ccccactcgt
     3061 ggcgggaata agatggcttt tgtggacaaa gacggcgttg aagtagagag ctcaactcac
     3121 tggatgccgc taccggaacc accgcaggag gtgaatcaat gacctggcct gaggcattca
     3181 ccacggtagg aattgcgatg gcggtggcgc tggtggtgta ttcgatttgc cgttggggtt
     3241 aacaaacaaa aaccccggat tgatggtccg gggtttttga aggaaacaaa acagaaacag
     3301 caattgccgt tacctgttgt taccatggca agtaaacgta tctcaggcga gcgcattgcg
     3361 ccgttctgac gcagataaat tagcctggat agaaggtgct ggcaataaaa aatagcgttt
     3421 tcttatcggt atcggtaaga ttgctgcggg tgcttg
//
LOCUS       DAJTYI010000220         3390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYI010000220 DAJTYI010000000
VERSION     DAJTYI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            799..1347
                     /locus_tag="OO564_003926"
     CDS             799..1347
                     /locus_tag="OO564_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6654831.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1065 a          615 c          755 g          955 t
ORIGIN      
        1 ttttcgaggc tatcagcttt gtcttgagcg taaataacat acaacacggg cacaactcct
       61 tgttcgggaa agttgtaaag tacgttattt gaaagggcga agatctgcaa cggaaagatg
      121 atgtctttgt taaggtcatg cataaagtaa gggtaattac gccaaaaatg acattttcac
      181 tgatcctgat cgtcttgcct tattgaatat gattgctatt tgcatttaaa atcgaggcct
      241 ggtttttcta ctgaaatgat tatgacttca ataacccttg aatgaagtct attcggtttt
      301 ctaggagacc tgttaaaaac aggacaatat caggtgaatg actatgcaat ttaatagtag
      361 atttccaagt atctaagcag ttttacacaa attctatgca gtatcggtat agtctctttt
      421 tagcataact tattgaaata tatagagaat ctgtgaagtc tcttttcagg gaactatgca
      481 gggtgttagt cgttcctctc tcgactccta gaaaagtgct atatacagga ctatataacg
      541 atatatagat gtttggtcac atggtgtaac tatcaagaaa cttaacgatt tcctacgtga
      601 aagttagaaa gtgatggtta tagttaccct cctaaaatct tcatagctcc ttcataacca
      661 gtcttaaaag gacttccttt tgagacactt tcaacgtaac ggtagaatgc accctcaagt
      721 agtctcaaaa ggttgttttt ggattatgag acagctaaac ttatgagact aacttttgag
      781 acttctaagg ggaacattat ggcggtgatt ggttattgca gggtatcaac acaagaccag
      841 actgtagaca accagaaact acagatagag aaggttcaca aggtcgataa atggtttatt
      901 gatgaggctg tctcaggagc tacaaaggcc accagcagag atggttttag aaacatgatg
      961 aactatgtaa ggtgtgacga tacgttagtt gtggtagcta ttgaccgttt agggcgtaat
     1021 accgttgatg tgctctctaa cgtggaggca ctacagacta aaggtgtgag ggttgtttct
     1081 ctacgtgaag gttttgactt attaacacca atcggtagag caatgcttac tatgatggct
     1141 ggtttagctg accttgaaaa agaccttatc agtgaacgta gaaaggctgg tattgaacgt
     1201 gctaaagctg aaggggtaca tatggggagg cctgtaaaag cttcttcaga ggctgtacag
     1261 acgcttattt cacaaggtaa gacgaggttg cagattcagg aagaattagg aatctctaga
     1321 gcaacttatt acagacttgc taaataagtt actgaatagt tacatggatg tagctgatat
     1381 ttggcttttt tgcaaagggg atcacatagc tggtgtaaca ggtacttagc agctacagac
     1441 atctgtcaga tatttttgaa gattctcaga gagggaattt gaagaggggc tactatgatt
     1501 ttataaaaat tttcagagtc tgaaaagtta ctgtacagat tttatacaaa ctaaaaagga
     1561 ctcccgtagg tgattcagta atgaattact tcacgaagag tccctttaca agtcgatttt
     1621 taatcgtggc tacgacaatc cacccatacc agacctaacc gtaccccacc ttaccaagcc
     1681 ataccctacc ccgccagact ataaggaagc gtagagaacc gatgaaggtg tctgcacagt
     1741 tgcatcttaa acaaccatca cagtgatgtc aaatagttct tttaagtagg aggggttccc
     1801 ctatggtcac atatcccctc tctcccgctt agtgtccctt gatagtatct agagagtatg
     1861 cctgtaatgt tagagagtat gcctgtaatg tttctgtatc aggtcagcta taagaggtct
     1921 ggatattacc ttgtaagatt cttggtagtg attatgcaga gggcttgata agagtgttta
     1981 acgggaggaa gagaaccatg aaaccgaagg taggttctat cttaacagtg actgtaagag
     2041 atacctgaaa aaatacttta acagaactac caagaagaga gggaacggaa gagagggaac
     2101 gataagacca gaggtagtga cctgttaaac aggctgtttc aaagtctctt aaaagtctgt
     2161 taagagggct aggtgaacaa tcctgccatt ttgtcaagtg ttttgtcttt agcaaaaagt
     2221 gctaaactta acaaaaagca ctaaatgcta aagagacgat taagaaatat actaagaggg
     2281 gcgcttcgct tggggtcact atgttctttc tctcccgtta agaggattta cagggacatt
     2341 tcaaagccct gataaggtaa gcccgatgat aacagaaaca gagggcaaaa ccacttatca
     2401 acactgtgaa cgaataacgt aaagggctgg ttaagaaagt cggtttaggg attggtaaca
     2461 ataccaatta gaagggagct tccctttctg gggtgtcgct ctatgggtga agttaacctt
     2521 tgaagttctc tgtgaagaaa ctgaacagct attatattgg ctaattacaa ccctttgaaa
     2581 gataagcatt tttagaagtt actgttaagg ttgcagagtt gtctgtgaag agacttgtca
     2641 gttctctata ttgggtggtt acaactcatt gaaaaataag agcttttaga ggttactgtc
     2701 agagaggtga atacttgata gatacagtct tccggttctg tcaggctatt ttttaacaga
     2761 tagcacaaaa tgctaaagat gcccctatgc catctcgtac aggggggttt tgaagggagt
     2821 ttaacggggg tctgtgtggt cattatcctt aaggtcaagc gtccatgtgc gaccgaacag
     2881 tttaaagaca acaagcactc tgacgaggac gtaacgaagt agcagatctg caataagtcc
     2941 aatacctcca ccagcccata catcaggtga ccccagctca ggagggagat ttaccattag
     3001 ttttccttgt aaggcgctct caatcgcatt ctgacacaaa aaagtacctg accttatgag
     3061 ttgacctgaa gaggtcattt aactcgttac agagcgattg aagaggtctg tgtgcaatgc
     3121 aataaccatg tgaccagata catagccacg cctcatattt tactctcccc ataaacccat
     3181 tacaccgcag atgttacgaa attgttaaaa atacttggta ttagtctttg aaaatgctag
     3241 cattgtatat ataagttaac agaataataa catgttatga ggggcagaat atgaatgaga
     3301 gacgcagaat ttagagcgat gcttcaggca tcaagagaac gcaacaagca caactcctac
     3361 gcttacacaa acaaccccac cagctacgaa
//
LOCUS       DAJTYI010000221         3336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYI010000221 DAJTYI010000000
VERSION     DAJTYI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(389..586)
                     /locus_tag="OO564_003927"
     CDS             complement(389..586)
                     /locus_tag="OO564_003927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654832.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1431..2480
                     /locus_tag="OO564_003928"
     CDS             1431..2480
                     /locus_tag="OO564_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6654833.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            complement(3253..>3336)
                     /locus_tag="OO564_003929"
     CDS             complement(3253..>3336)
                     /locus_tag="OO564_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6654834.1"
                     /translation="LLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT         1084 a          596 c          562 g         1094 t
ORIGIN      
        1 aacagatagc tgtagtcaat gtattattat gcaccacact gttaattttt tgcaaaacaa
       61 acaattacaa tacaaaacca ttattaaatg cgatggggat ttcactatta gttattattt
      121 atattgcaac ttgcccagga aataaagtca ggtattatgc agagatagaa cattggttta
      181 aagagtatgg taattttaat ataatccaaa gatctatgct tggacttaac ttatatgcag
      241 atatgttatt ttctgtaaaa tcaattatcc cagcattact agctttttcc agtactatta
      301 tatgcaagaa aaaaacaaaa atactccctc taatttcttg ctgtatcttg atcacactat
      361 atattataca tacaccgcca gttatttttc aagccataca tttttctgaa agcaatcttt
      421 tctcgacact ctccgtgttt agggtgtctt ttgccatgat acttacagca ctcattatat
      481 ttccaaccgt aatatcactt aattttaatg tcacttcaat tttcatatcc acaatgatta
      541 ttggaaccat tgccacaaca tctatgttag gattatcacc aagcatatat gcttctggta
      601 atagaattta ttttattcca tatttgcttc ttataacagc aattgttgtt tcaaccccaa
      661 tcgcaataaa taacatagta gcaaattttg ccaagagcca ttataaaatt tgaattaaat
      721 ttaaaagcca cctccttttt ttgggggggc ttagaaataa aaacaaattt aattggactt
      781 ttattggtta taacacattc ctctatactg ctcatcaccc gactatacta ggattttcgt
      841 cattttttgc tgaaccgctc tcatgaaatc ctgacaacaa agtcatgttt cacgtcagga
      901 tacttcttct ggcatggaaa tataccgtgt gcagctgatg catatttata tttcaatata
      961 atacgatatt gaatggccta caccactggt agttcaggcc aaatgacatc cgacggtttc
     1021 acgatgaact acggcaaaag aatatcagag ccctcattcc tccccgaaag ggagcgggct
     1081 ctggtctggt gagtatgcag atcgtaaagg ctatggccat ggtgcgagag tggcagctat
     1141 gccagaaaga gtgtgtattg cctgagactc taacctgatg cagagacgat tgtcttgaac
     1201 ctgatttatt caacaaagcc gctggcagga tgttacgaaa gttatcgcgg ccgcaggctg
     1261 atttgatatc gatactatcg gtggtactgc ggaagtaaat gcctatatag tgcgactttg
     1321 aaaaataata aattaatatt gaactcgttg attaataatc ataaactttc aggcattcat
     1381 atttgcaatg agtgtttgaa aatcacacaa aactggagag cctactttaa atgtataata
     1441 acaaaatcga caacaacaga aaaacaaggc atgattgggt tgatgcttta aaatttctcg
     1501 gtatttttgc tatatattta ggtcaccttg gtttgggggc tggcaaattg tatccttttg
     1561 tttttagtta tcacgtccca ctattctttt tcgctgctgg tttttttact atcaaaaaaa
     1621 atgacttatc tattttcgat tatatcaagt caaagtttta cagattaatg attccatatt
     1681 tcacttttgc atttactata ttaataataa acacaattaa ctcaggtgag acgattgatt
     1741 atatatatag ccacatttat gatataatat atggtgttag aaacaaccaa tttgtaggaa
     1801 caatatggtt tataaactgc ctttttgtta taatagcaat tgatgcaatc tttaaagaaa
     1861 tagtcaagaa caatatagtg attttaatca tatcactatt atcgttcatg ttgtcacaaa
     1921 cagtgctcaa tcataaccca ttactagatc ctcaatggtt ttggaacata gactccgcta
     1981 tggcatactg gtggttattg cctctcggta gatgtatgtt tttagaatta acacgtgaca
     2041 ggttttttgg aaaatctaaa attggtttca ttgtcttctc aattaccgcg ataatgagtg
     2101 cgtatcaact ccttaaccag aaacctttgc tttttaagat tatctcaata ttcaatgcag
     2161 acataatatc aagtagttat attcaagcta ttaatactat tatcaccacg gtaggcttga
     2221 taatatttaa tatttttata gcaaaaataa tttgtgggaa tgactttatt gtaagagctg
     2281 gaagaaatac tcttaacata tgtggaatgg agtatatcac caaattattt atccctatgg
     2341 ctttagcaat cataggcttc agcgtgacca ttccaaatcc tatctgcgca atcatatata
     2401 cctgtatttg tgtatatgta tcagataaaa ttggccattg gctatcgagg acagtcggtg
     2461 ggccattttt gataaaatga taaaaaggcg caattaattg cgccttttta tagatatata
     2521 gcgcatattt atgctcagat tatactgtta aaattaatgc gcaaatgtta cggttttctt
     2581 tcctagcaac tgaagggaat gttcgaagtg caaacataaa attacccctt cactgaacag
     2641 atattcagtc ggccaacaca tgagaaagaa atacaccaga tgaattaaaa tatcatatac
     2701 ttattaatca aagaaataaa agcataaaca gtaggtggtg acgctgataa actggctata
     2761 aacaacctgg aaggtgatga gctatcgcaa tataatttat tggtgaatta tttgaagccg
     2821 attgataaca ccagtgctca aaagactccc cccggtaacc ataagtagct aatatctagt
     2881 acagtttcga tatctattat cgttactgcg tcgatatagt ctaaatagct gcacctaatt
     2941 ctgtcacact tgtcgtttac agatgggtaa tgtgttgcgt atgggcaaca aaactcaagc
     3001 cagatataac acaacctagc tgagaacgcc ttcctgatgc ttcgcccagc aagggctgag
     3061 cgaaagtgga gcacttttct gcgcagctat ttaggacagt attgagtcat attgtttatg
     3121 tatgagttaa gtttcgtaat atcaattaaa taaaataagt tgaaatcatt gtagaagtga
     3181 gcattatacc caggctacct gaacctactg aaaaatgagt attattccaa caattatcct
     3241 agattaacaa gtctatttat ttggatgcat aggccactcg ggttttacta cattaacacg
     3301 cataagcagc acccgatatt ttttccatgc cagtaa
//
LOCUS       DAJTYI010000222         3312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYI010000222 DAJTYI010000000
VERSION     DAJTYI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..866)
                     /locus_tag="OO564_003930"
     CDS             complement(<1..866)
                     /locus_tag="OO564_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654835.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDA"
     gene            complement(894..1814)
                     /gene="rbsK"
                     /locus_tag="OO564_003931"
     CDS             complement(894..1814)
                     /gene="rbsK"
                     /locus_tag="OO564_003931"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6654836.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2120..2902
                     /locus_tag="OO564_003932"
     CDS             2120..2902
                     /locus_tag="OO564_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6654837.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2904..3130)
                     /locus_tag="OO564_003933"
                     /pseudo
     CDS             complement(2904..3130)
                     /locus_tag="OO564_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          976 a          763 c          655 g          918 t
ORIGIN      
        1 gcatcacgtt cgttgatatc gcccaactcc agtgccagac ggatggtaaa tgaccatact
       61 gcgacctgca tacccacata aaggaattga gccacaatac cacgacgaaa gcgcgaattt
      121 ttagccagat agcgcagcgt atccattgct gacgggcgtt tatggtgact tgtctgtgcc
      181 actttacagg ttgggaagcg ggttaaaagg aacaacacca tgaccacaac cagaatcata
      241 atcatatact tatacggttc aagggtgttc tctaacatca gcaccttaaa gttgtgaatt
      301 tgctcggcgt tcattccgga catctgcttc tcaaggcttt ccccctcgga gaaaaccaga
      361 tatttgccca ataaaatacc agacgcagca ccaatcggat aaaaggtctg gctgatattg
      421 agccgcaatg tggcataggc ttttggaccg atcattgaac tgtatgtgtt cgctgcagtt
      481 tcaaggaaac tcaggccaat cgcaatcgca aaaatagctg caaggaacat agtgtaggtt
      541 gccatatgcg aggcagggaa aaaaagtgta caaccaccaa tatacagcgt caggccaatt
      601 aaaattgcca ccttataact ggtcttttta atcacaaggg atgctggtat tgcaattaaa
      661 aaataacctc cataaaatgc gctctgcacc aatgctgaag caaagttact tagcgaaaat
      721 acacttttga attgagtgat taatatgtca tttaatgcag ctgcgcatcc ccatagcggg
      781 aataaacacg ataacaaaat aaactggaac aagggagtct tattcagata cccatcaggc
      841 atctgaatga tgtttttatc gttcatagtg ctacctttaa ctgtgcagga tgattattcg
      901 tttaaggtta aaaattcatt aaattgttca atactcggat aagatgattg catacctttc
      961 cctgtgacgc tgaaagcggc aaagagagcg gcttttttca aagcggcttc aacatcaccg
     1021 ctttgaacat aataatggga aaagcaacca ataaatgcgt caccagcgcc actagtatca
     1081 acagcattta ctttgaatgc aggaacatgg acttcctgat cgcgggtcat ccataatgcg
     1141 cctttttcgc tcatggtaac aataatattg ttcagccctt tatcaactaa cgaacgtgcg
     1201 gccaaacgaa tatgatcata agtatcaacc gacataccag ttaatatttc cagttctgtt
     1261 tcattcggga taaagaaatc acctttgcag gcataagaca tatctaactc acgcaatgcc
     1321 ggagccggat ttaataacac ttcaatacca tttttcttac caaactcaat cgcgtggtaa
     1381 actgtttcca gttgaacttc cagttgtaaa acgatcaatt tgcatttttt cagatcttct
     1441 gcagctcgat cgatatcttc cggggaaaga aatttattcg ctcccttaat tattaatata
     1501 ctattgctcg agttggcatt aacaaagatc ggtgcaacac cactgctggt acaggggacc
     1561 ttctcaacat aagtggtatt aattccccat gattcaagat tacgaatagt attatccgca
     1621 aaaatatcat cacctacttt agtcagcatc aggacttttg aattcaattt agccgccgcc
     1681 accgcttgat tagcaccttt cccaccacat ccgattttga aggcaggtgc ttccagagtt
     1741 tctccttctt taggcatctg attagtgtaa gtaatgagat ccaccatatt ggaaccaata
     1801 actgcaatgt ccatttcact acctcttata aactttcgca taacaatggt atttaaataa
     1861 cattagcatg ttacttttgc atcatttgtg actgagatcg cgattagcac atcaacccga
     1921 tgtttattta atagacttcc agtctcatca ctcaggccaa cactatctaa tcataagcaa
     1981 cctaacaaga ttagtgccca aaattcagca gcctataccc ttttcatttc aaaggggcgg
     2041 tcgtatagta tggtaatgaa aacaatgttt actaacgcca aaatgttatt tttataacat
     2101 tcttacggag agagagttga tggaaacgaa gcaaaaagag cgtatccgac gtttgatgga
     2161 actgcttaag aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc
     2221 tgtaatgact attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact
     2281 gggtggctat attgtaatgg tgaataaacc cgcgctatcc atgccagtaa tccatgacgt
     2341 tccaaaaaat catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga
     2401 aaatgatctg atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc
     2461 ggatgcaatc accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga
     2521 aaagcctaat gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt
     2581 ttacgatgcc agtaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc
     2641 cgccagcggt gtgcataatc actttggcgt cagctggttt aaccctgaag atcttgccac
     2701 taagcgtaaa gcgatgaacc gtggactacg gaaaattttg ctcgcccgcc acgcgttgtt
     2761 cgatgaagtg gcctctgcca gcctcgcacc gatctctgca tttgacgttc tgattagcga
     2821 tcgtccgtta ccggcagatt atgttacgca ctgccagaat ggttctgtaa agatcattac
     2881 acctgattca gaagacgaat gacttactga aaaaacacca caatcttgtt aaacatcgtc
     2941 gggttggact gattacgttg cactttcacc acatattcca gcttatctat ttggcttatc
     3001 acctactcca gacgctggtc atccttgacc agtagccaga tatggctttt gtcgcagtcc
     3061 tgaatcggca ggcagagaat gtcttcaacg ttaagagcgc gacgggcaaa agcccacaaa
     3121 ggtgagtcat tacacccgga tggtaaacag gcacttcctt gttgtagaag ccgttatatt
     3181 gtccataatg tctgggtttt taatgcatac tgggtaacaa ccggacgaat cttcgaatat
     3241 cactggcgct gccagccggt ttgaattcat ctcacaaccc tgcatagagc gaatctcttg
     3301 cctgcacgtc ac
//
LOCUS       DAJTYI010000223         3220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYI010000223 DAJTYI010000000
VERSION     DAJTYI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..962
                     /locus_tag="OO564_003934"
     CDS             <1..962
                     /locus_tag="OO564_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR6654838.1"
                     /translation="VFVFFYFAILMTVSAGMPLVGCVLTLLLLVLLIFMAAKGLRYQA
                     LMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVV
                     LMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILTFLDLLP
                     FLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYL
                     TVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMID
                     WQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
     gene            complement(1016..>3220)
                     /gene="valS"
                     /locus_tag="OO564_003935"
     CDS             complement(1016..>3220)
                     /gene="valS"
                     /locus_tag="OO564_003935"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6654839.1"
                     /translation="VVATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPI
                     VGDEHADMEKGTGCVKITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGN
                     ESDVYSSEIPAEFQKLERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPM
                     LTDQWYVRADVLAKPAVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRI
                     PAWYDEAGNVYVGRNEEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENT
                     DALRQFHPTSVMVSGFDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDE
                     GQKMSKSKGNVIDPLDMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPH
                     GTDALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGE
                     MTLSLADRWILAEFNQTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMN
                     GGTEAELRGTRHTLVTVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQ
                     YDASQVDEAALADTEWLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENR
                     GFLQTLARLESITVLPADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKI
                     EGEISRIENKLANEGFVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
BASE COUNT          713 a          792 c          813 g          902 t
ORIGIN      
        1 ttgtttttgt ttttttctat ttcgcaatct taatgacagt gtcagcaggt atgccgcttg
       61 ttggctgtgt tttgacttta ttactgttgg ttttgcttat atttatggca gcaaaaggac
      121 tgcgttatca ggccttaatg accagtctca acagcgtaag atttagtttt aattgctctt
      181 tgaaagggtt ctggtgggta acctttttct tgccgatttt aatggccatt gggatgggga
      241 ctgttttctt tatctcgaca aagatgctac atgccaatag ttcaagtagt gttattatat
      301 ctgtggttct gatggcaata gttggtattg tttccattgg tatttttaat ggtactttat
      361 atagcctggt aatgagtttt ctctggagca ataccagttt cggtatacat cgtttcaagg
      421 tgaaattaga tactgcgtat tgtataaaat atgccatcct cacattttta gatttattac
      481 cttttctcgc tgttgctggt tatattatct tcgatcaaat attaaatgag tatgatagtt
      541 ctgggtatgc aaatgatgat attgagaatt tacagcaatt tatggaaatg caacgtaaaa
      601 tgataatcgc gcagttaatt tattattttg ggattgctgt tagcaccagt tatttaacgg
      661 tgtcgttgcg aaatcatttt atgagcaacc tgtcactgaa tgatgggcgt attcgttttc
      721 gctcaacttt aacgtaccac ggtatgcttt atcgcatgtg tgcgttggtg gtgatatccg
      781 ggattacggg tggtctggct tacccactgc tgaaaatatg gatgattgac tggcaggcaa
      841 aaaatacgta tttgctgggc gatttggatg accttccttt aatcaataaa gaagaacaac
      901 cagataaagg cttcttagcc agtatttctc ggggagtcat gccttcttta ccatttctgt
      961 aagagataaa aaaggccgga gcatgctccg gccttcgttt tcatcactgt gttgattaca
     1021 gtgcggcgat aacagcctgc tgttcaatca gcttggcttt cgcttctgca taaccttcca
     1081 gcttctcacg ctctttcgcg ataaccgctt ccggtgcgcg ggcaacaaag ccttcatttg
     1141 ccagcttgtt ctcgatgcgg ctgatttcac cttcgatctt cgccacttct ttcgccaggc
     1201 gcgccagctc atcttctttg ttgatgaggc cagccatcgg gatcagcagc tctgcgccgt
     1261 cgatgatctt ggtaacggaa accggacctt tgtcatcggc aggcagcacg gtgatgcttt
     1321 ccagacgcgc cagcgtttgc aggaagccac ggttttcatt tacgcgacgt tctgcatccg
     1381 cgctgcaacc acgcagcagc agctccagcg gtttgcccgg cgcgatgttc atttctgcac
     1441 ggatgttacg tacagcaacg atcgcctgtt tcagccactc ggtgtcggcc agtgcggctt
     1501 catcaacctg agatgcatca tactgcggga acggctgcag catgatggtg tcggcagtga
     1561 taccgcaaag tactttcaca cgctgccaga tggtttcggt gatgaacgga atgatcggat
     1621 gcgcgaggcg cagcagacct tccagtacag tcaccagcgt atggcgagta ccgcgcagtt
     1681 cggcttctgt tccaccgttc attaccggct tggtcagctc cagataccag tcgcagaact
     1741 ggttccaggt gaactcatac aaaatgcctg cggcgatatc gaagcggaag ctgtccagcg
     1801 cttcgcggta cgctttgata gtctggttga actccgccag aatccagcgg tccgccagcg
     1861 atagcgtcat ttcgccgccg ttgaagccgc aatcctgacc ttctgtgttc atcagcacaa
     1921 agcggctggc gttccacagc ttgttacaga agttacggta gccttccaga cgcttcatat
     1981 cccagttgat gtcacgaccg gtagacgcca gcgccgccag ggtgaagcgc agggcatcgg
     2041 tgccgtgcgg ctcaatgccg ttcgggaact gtttctcggt gcgcttacgg attttgtccg
     2101 ccagctgcgg ctgcatcata ttgccggtgc gtttttccag cagttctggc agcgaaatac
     2161 cgtcaaccat atccagcggg tcgataacgt tacccttgga tttggacatc ttctgacctt
     2221 cgtcgtcacg aatcagaccg gtcatgtaaa cagtgtggaa cggcacctgc ggtttgccat
     2281 tttcatcttt gatgaagtgc atggtcatca tgatcatgcg ggcaatccag aagaagatga
     2341 tgtcgaaacc agataccatc acgctggttg ggtggaactg acgcagggcg tcggtatttt
     2401 ccggccagcc aagggtagag aaggtccaca gtgcagaaga gaaccaggtg tcgagaacgt
     2461 cttcgtcctg acgcagggca acgttggcac cgaggttatt ttccttgcgc acttcttctt
     2521 cgttgcggcc aacataaaca ttacccgctt cgtcatacca tgccgggata cggtgacccc
     2581 accacagctg acgagagata caccagtcct gaatatcgcg catccaggag aagtacatgt
     2641 tttcgtactg cttcggtacg aactgaatgt cgccgttctc aaccgcttca actgccggtt
     2701 tcgccagtac atcggcacgc acgtaccact ggtcggtcag catcggttcg ataaccacgc
     2761 cgccacggtc gccgtaagga acggtcaggt cgtgcggttt aatttcttcc agcaggccga
     2821 gtgcatcaac cgcagcaacg actgctttac gtgcagcaaa acgctcgagt ttctggaact
     2881 ctgccgggat ctcgctggaa taaacgtcag attcgttacc tttggtatcg aacacctggg
     2941 cgctttcacg gatatcaccg tcaaaggtca ggatgttgat catcggcagg gcgtgacgtt
     3001 taccgacttc atagtcgtta aagtcgtgcg ccggggtgat cttcacgcag ccggtgcctt
     3061 tttccatgtc ggcgtgttcg tcgccaacga tcggaatacg acggttaacc agcggcagaa
     3121 tgacatattt gccaatcaga tctttgtaac gcggatcttc cgggttaaca gctacaccgg
     3181 tatcgcccag cagggtttcc gggcgagtgg tcgcaaccac
//
LOCUS       DAJTYI010000224         3144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYI010000224 DAJTYI010000000
VERSION     DAJTYI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            397..591
                     /locus_tag="OO564_003936"
     CDS             397..591
                     /locus_tag="OO564_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6654840.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(1616..2725)
                     /gene="fepE"
                     /locus_tag="OO564_003937"
     CDS             complement(1616..2725)
                     /gene="fepE"
                     /locus_tag="OO564_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6654841.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          930 a          770 c          652 g          792 t
ORIGIN      
        1 gttggcgcag ctacaacacc gcgccgacac cgctttttta aatatcacaa agagagcaag
       61 aagaactaat ttgtcataac tctctattta taaagaaaaa tcagcaaaaa cttgtttttg
      121 cgtggggtgt gggcatttca ggcatgagaa cagactcgcg tttttccgga gtatcagcgg
      181 aattattgat taaggaacac tcgacgcaga gcgaaatttt atgcaacagc ggctgcactg
      241 cacagggatt atttatcagt tgatatgctg tgccatgata cgtcctaaag aaacaggaca
      301 ggatgtgcaa ttgtaatacc gtcgcccgca acgtcttaca attaccacct gctcagcccc
      361 cccccgacac ccggaactga cggagaacaa cggaaggtga acaaacaaca acaaactgcg
      421 ctgaatatgg cgcgatgtat caaaagccag agcctgacac tgcttgaaaa actggatgct
      481 ctggatgccg acgaacaggc ggccatgtgc aaacgactgc acgaactcgc agaagaactc
      541 cagaacagca tccagattcg ctttgaggcc gaaagtgaaa taggaacata acgaagctga
      601 cggaggcggt cacggctttc ccgtgggcgt tttcgtcagc agacaagccc gtcagggcgc
      661 gtcagggggt tttagcaggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      721 agtatatact ggcttaatca tgcggcggca gagcaaatgg tgagagaaca gcgacatatg
      781 aggtgtgaaa tgctgcacag atacgtaagg agaaaatgca gcttccggcg ctcttccgct
      841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt gtctgctcac
      901 gcaaagacgg atatacggtt atccacagaa tcaggggata acaccagaaa aaacatgtga
      961 gcaaaaaaga ggccccgaaa aaaggccgcg ccggtggcgt ttttccatag gctccgcccc
     1021 cctgacgagc atcacaaaaa ccgacgctca agtcagaggt ggcgaaaccc gacaggactt
     1081 aaagatacca ggcgtttccc cctggtggct ccctcgtgcg ctctcctgtt cctgcctttc
     1141 ggtttaccgg tgtcattccg ctgttatggc cgcgttcgtc tcattccacg cctgacactc
     1201 agttccgggt aggcagttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1321 acgccattgg cagcggccac tggtaaacag gatgtgcgag agatgtattg agagttcttg
     1381 aagtggcagc cttactgagg ctacactaga aggacagatt tggtgacatg tctcgcacaa
     1441 gactgttacc acggttcaga agttccccaa cttactgaac cttcgaaaaa ccacctcccc
     1501 aggtggtttt ttcgttttct agagtagaag actacggctg taagggaatg atctcaagaa
     1561 gatcctttga actttttata aaagttgctt ataaaatgat gcatcagcac aacttttact
     1621 caatcacact catctttctg gcctccatgg catgacgtaa aagtatatag ccacatgcaa
     1681 gcatccctcc aatgacagaa gaaagaatta caattatggc attacttggc ccatcctttc
     1741 tgaccggtaa cgttgggctc aattgatact gaaatggcat aaaatcaaca tcccccactt
     1801 tcgccacaac caattgattc acaacatatt gacgatttcg caactcccca ctcaattccg
     1861 aaacatcctt gattgatttt ttgatgttca atttagttgc tataccatca gaaccgagag
     1921 ccacaggaaa atctgggtca tccttcataa tctgaccatt gctgtataca ggttttttta
     1981 ttccagccgc attagcaacc tccagtgaat aattgaggcg tcgaatatct gcattcagct
     2041 ggtttctaat ttttatctca tccagtgcaa gaatttcttt ttcaacatta gttttaactt
     2101 ctagtttatt tcttatatct tccatcaaat cccttacaac aagggaagaa acataattaa
     2161 tatacccaga caacacatcg tgagcttctt tacttgttgg agcagtaaaa ctcagagtcc
     2221 atgatacata caatgatgac ttgtcgtttt tcttactggc attatcatca atcgacttca
     2281 tatttttact caactcaaca attgcacgat gcagttcgag agcgctaatg ttattctttt
     2341 taataatctc cataacataa ggagatgatc tcatatactc attcaacgaa ttaacggact
     2401 gaaatttctt aataaacaga gcaaatgcgc tatcacgatt gatatcaaaa tccactccca
     2461 gaacatgaag cttggaaagc tccttattta attcactcca ttgaacagac tcagcaggag
     2521 taaccacagc agaactagtc catttttgtg gcaaaacaaa agaaacaaga aagccaatac
     2581 aagcaaaaaa tagaatcacc ccaacaattt ttatttttgc tgcccataaa acgctaatca
     2641 agccaaaaag gtcaatttct tcattaccat tcggcaacat gttgtaatta gaaaaatgtt
     2701 cgtcagagac ctttgagatt ttcattgaaa aaaacacaat atattaacat atggtttttt
     2761 tattctatca tatgttagga aaaaacctaa aaaccattca gcagacattt aacataatat
     2821 cttattgcgc gcaccgctaa aacgcgctga acgcgattct gccgcaaaca ccgcaaaaag
     2881 agatgttttc cccggggtaa accggaaaaa tgcgtcagga tcgctttcag ctcactgcat
     2941 agctatgcat gaaagtgaat gacgatcggt ttgggcgcat acagtgccca tgagacacat
     3001 tttcggacat tttcagttcg tgaatccctg actcattgct acatatctga acagacaccc
     3061 ctagcagcgc ccccaggggc gtcctgtaaa aaaacacacc gcgccgctag cagcccccct
     3121 aatataagat gatgtttttt tatg
//
LOCUS       DAJTYI010000225         3134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYI010000225 DAJTYI010000000
VERSION     DAJTYI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO564_003938"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO564_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6654842.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="OO564_003939"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="OO564_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO564_003940"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO564_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6654843.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO564_003941"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO564_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6654844.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3134)
                     /locus_tag="OO564_003942"
                     /pseudo
     CDS             complement(2194..3134)
                     /locus_tag="OO564_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          779 a          761 c          758 g          836 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccaag gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggc
//
LOCUS       DAJTYI010000226         3050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYI010000226 DAJTYI010000000
VERSION     DAJTYI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO564_003943"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO564_003943"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6654845.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO564_003944"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO564_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6654846.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3050)
                     /gene="bcsE"
                     /locus_tag="OO564_003945"
                     /pseudo
     CDS             complement(1963..3050)
                     /gene="bcsE"
                     /locus_tag="OO564_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          764 a          786 c          805 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa
//
LOCUS       DAJTYI010000227         3036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYI010000227 DAJTYI010000000
VERSION     DAJTYI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            142..1143
                     /gene="gap"
                     /locus_tag="OO564_003946"
     CDS             142..1143
                     /gene="gap"
                     /locus_tag="OO564_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6654847.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(1185..2624)
                     /gene="aldA"
                     /locus_tag="OO564_003947"
     CDS             complement(1185..2624)
                     /gene="aldA"
                     /locus_tag="OO564_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6654848.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2821..>3036)
                     /gene="ydcF"
                     /locus_tag="OO564_003948"
     CDS             complement(2821..>3036)
                     /gene="ydcF"
                     /locus_tag="OO564_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCR6654849.1"
                     /translation="INQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDF
                     PAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          792 a          779 c          700 g          765 t
ORIGIN      
        1 ctcaaaattt cattaaatat tgttcacccg ttttcagtac gttacgtttc actgtaagaa
       61 tggagattgc ccatcccaca atcctggtct aagcctggaa aggatcaatt ttcatccgaa
      121 cgttcctgac aggagaaaac catgagtaaa gttggtatta acggttttgg tcgtatcggt
      181 cgactggtgt tgcgtcgttt acttgaagtc aaaagcaaca tagacgttgt cgctattaat
      241 gatcttactt ccccaaaaat tctcgcctat ctgctgaaac atgattcaaa ctatggtccg
      301 ttcccctgga gcgttgattt tacggaagat tcacttatcg ttgatgggaa aagtatcgcg
      361 gtttacgccg aaaaagaggc taaaaatatt ccgtggaaag cgaaaggggc agaaatcatt
      421 gtcgaatgta ctggctttta tacctccgcc gataaatcgc aggcgcatct tgatgctggt
      481 gcgaagaagg tgttgatttc cgctcctgcc ggtgaaatga aaaccatcgt ttataacgtt
      541 aatgacaaca ctctggatgg caacgacacc attgtttccg tggcgtcatg caccactaac
      601 tgccttgcgc cgatggccaa agccttgcat gacagtttcg ggatagaagt cggcacgatg
      661 acgaccattc atgcctatac tggcacccag tcactggtgg atggcccgcg tggtaaagat
      721 ttacgtgctt cacgcgcagc ggcagaaaat atcattcccc acactacggg ggcggcaaaa
      781 gccattggtc tggtgatccc ggaactgagc ggcaaactga aaggtcatgc gcaacgcgtg
      841 ccggtgaaaa caggttcggt cactgagctg gtgtccattc tcggaaaaaa agtgactgcc
      901 gaagaggtga ataacgcact taaacaagca accaccaata acgagtcatt tggttatacc
      961 gatgaagaaa tagtctcttc cgatatcatt ggcagccatt tcggttcagt gtttgatgcc
     1021 acgcaaacgg aaattaccgc cgtgggcgat ttacaactgg tgaaaacggt cgcctggtac
     1081 gataacgaat atggcttcgt catgcagctt attcgcaccc tcgaaaaatt cgctaaactc
     1141 tgacgcgcac aggcggagga aaaaacctcc gcctctttca ctcattaaga ctgtaaataa
     1201 accacctggg tctgcagata ttcatgcagg ccatgtttac catctgcgcc gccaataccg
     1261 gatttacgcc atccggcgtg gaaaccttgc atagcttcga agttttcacg gttgatgtaa
     1321 gtttcaccaa acttcagccc tttaatggct ttcatcgcga cgttcagatt ttgggtatag
     1381 attgatgagg tcaggccgta atcactgtca ttagccattg agatagcatc ttccagcgtg
     1441 tcaaatgcga caactggcag caccgggcca aaggtttcct catgcataat cgacatttcc
     1501 tggcgaacat ccagcagcaa tgtcggcgga taataatatc ctttcccctc taccgctttg
     1561 ccacccaacg ccactctcgc cccttcttct actgcgcgcg ccactttttg ctcaaccctt
     1621 tccagcgccg cggcgttaat caacggcccc atcgcaatgt cgttgcgttc agcggggtta
     1681 ccaaattgaa ccgcctgcaa cgcttcaccc agccgattga cgaactgatc ataaatgcct
     1741 ttctgtacat aaacacgttc tgcacagtta cacacttgcc cactattaat gacgcgtgaa
     1801 tcaacgatgg ctttgactgc cagttcaaga tcggcatcgt ccattacgat agccggtgct
     1861 ttaccaccca attccagaca cactttggtg atgtttttcg ccgcagtcgc catgatcttc
     1921 tcacctgcag agacgctgcc tgtcatactg accattgcga cctttgggtt acccgccagt
     1981 tcttgcccaa cggtttcacc acgccccagt acaaggttaa acacgccgcg cggaaggcct
     2041 atttcatcga cgattttggc gaatgcaatc gcattgtttg gcgtaaattc actgggttta
     2101 atgacgatgg tattaccggt caaaagagcg ggagccattt tgcgggcaat gaggaagaac
     2161 ggaaagttcc acggcagaat gccggtagtc acaccaagcg cacgtttaaa cagaagaata
     2221 ttttctcctg gacgatcgct ttgaataatc tcgccctcgt aacgccgtgc ccactccgcc
     2281 atgtaatcga tatagtcggc agtaaaagcc acttcgactt cagctagctg ctggatcttg
     2341 cccccttctt caacaatcag cgcactgatt tcactggcgc gttcgcggat cccggcggag
     2401 attttgcaca accaactggc gcgttcaata gcaggcaacg cttcccattc tggttgtgca
     2461 cgttctgctg catcgattgc cttacgggca tcctcggcct gaccatcggg tatgcgggaa
     2521 atgacagcct ctgtagcagg gtttaccaca tcaatccatg cgtctccacg ccaggtaaca
     2581 aactgtccat cgatatacat aggatgttga acgggtactg acatgggcga ctcctgtgat
     2641 ttatatgttt gttttcggtg aatatattgt taattaacat caatcatcag agagacggca
     2701 tcgggaaagg acaattctgt ggagggcttc ataaaaacaa gtgtaaaagg aataaaaggc
     2761 agaaataaaa gtcaggagtc atgcccgttt gcaaacaagt ggttcacaaa cgggcaattt
     2821 ttaacgtaac gagcgacttt ccatcgcctc gatgagcacc gcatcatgtt tcagcgtttg
     2881 ccatgcatgg atgacttctg ccggaaaatc aacgtgaacg ataaaatctc gcccgcgggg
     2941 accgtagcca tcgctatcat cgtgtaaacg cggcagctcg ccagtgagta gtgagagata
     3001 acgctcaacc ggccataatc cttgtaactg attaat
//
LOCUS       DAJTYI010000228         2894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYI010000228 DAJTYI010000000
VERSION     DAJTYI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..>2894
                     /locus_tag="OO564_003949"
     rRNA            1..>2894
                     /locus_tag="OO564_003949"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          762 a          634 c          910 g          588 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgttct
     1201 gtaagcctgt gaaggtgtac tgtgaggtat gctggaggta tcagaagtgc gaatgctgac
     1261 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1321 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1381 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1441 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1501 aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1561 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1621 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1681 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg ctcgtggagc
     1741 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1801 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1861 ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1921 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1981 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     2041 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     2101 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     2161 gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg
     2221 cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga
     2281 gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag
     2341 cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt
     2401 tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata
     2461 ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc
     2521 tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg
     2581 ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg
     2641 ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat
     2701 gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca
     2761 cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa
     2821 gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact
     2881 aatgaaccgt gagg
//
LOCUS       DAJTYI010000229         2799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYI010000229 DAJTYI010000000
VERSION     DAJTYI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(26..101)
                     /locus_tag="OO564_003950"
     tRNA            complement(26..101)
                     /locus_tag="OO564_003950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Val,seq:tac)
     gene            360..1775
                     /gene="gltX"
                     /locus_tag="OO564_003951"
     CDS             360..1775
                     /gene="gltX"
                     /locus_tag="OO564_003951"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6654850.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1827..2219)
                     /locus_tag="OO564_003952"
     CDS             complement(1827..2219)
                     /locus_tag="OO564_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6654851.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2221..2580)
                     /locus_tag="OO564_003953"
     CDS             complement(2221..2580)
                     /locus_tag="OO564_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6654852.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          690 a          679 c          703 g          727 t
ORIGIN      
        1 cacggcggag actacataaa gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc
       61 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cccgctgtgt ggagtcgcat
      121 tatagggaga gttcaaaatg agtcaacgca ttttctaaag aaattgttcg ttcgtcgtaa
      181 atttaagcaa gatgatcgca aaacagaccg tgttgcgcaa tttgtcaacg aaaacaataa
      241 tgcgtaaggt agaaacccga actacattga ggaatcaggc gggagtgata gaatatcgcc
      301 cactcaattt ttccaggatt tgccggttgt cggcatcttt ctaaacgtaa ggccatttca
      361 tgaaaatcaa aactcgcttc gcgccaagcc caacaggcta tctgcacgtt ggcggcgcgc
      421 gtactgctct ttactcctgg ctttttgcac gtaaccacgg cggtgagttc gtgctgcgta
      481 ttgaagacac cgatcttgag cgttccacgc cggaagctat cgaagccatt atggatggca
      541 tgaactggct gagcctggag tgggatgaag gtccgtacta ccagaccaaa cgttttgatc
      601 gctacaacgc ggtgatcgat cagatgctgg aagagggcac tgcttataaa tgctattgct
      661 ctaaagagcg cctggaagcg ctgcgcgaag agcaaatggc gaaaggtgag aagccgcgtt
      721 atgacggtcg ctgccgccac agccatgagc atcatgctga tgatgaaccg tgtgttgtac
      781 gttttgctaa cccgcaggaa ggttctgttg tttttgacga tcagatccgt ggtccgatcg
      841 agttcagcaa ccaggaactg gacgatctta ttatccgccg taccgatggt tccccaacct
      901 ataacttctg tgtggttgtc gatgactggg atatggaaat cacccacgtt atccgtggcg
      961 aagaccatat caacaacacg ccgcgccaga tcaacattct taaggccctg aaagcgccgg
     1021 tgccggttta cgcgcacgtt tctatgatca atggcgatga cggtaaaaaa ctgtccaaac
     1081 gtcacggggc agtcagcgta atgcagtatc gtgatgacgg ttatttgcca gaagcactgc
     1141 tgaactatct ggtgcgtctg ggctggtccc acggcgatca ggaaatcttc actcgtgaag
     1201 agatgatcaa atacttcact ttgaatgccg tcagcaaatc tgccagtgcg ttcaacaccg
     1261 acaagctgct gtggctgaac catcactaca ttaacgcgct gccgccggag tatgttgcta
     1321 ctcacttaca gtggcacatt gagcaggaaa atatcgatac ccgtaacggc ccgcagctgg
     1381 ctgatctggt gaaactgctg ggcgaacgct gcaagacgct gaaagagatg gcacagagct
     1441 gccgttattt ctacgaagat tttgctgagt tcgatgccga tgcggcgaaa aaacatctgc
     1501 gtccggtagc gcgtcagccg ctggaagtgg ttcgtgacaa actggccgcg attactgact
     1561 ggaccgctga aaacgttcat cacgctattc aggcgacggc ggatgagctg gaagtgggta
     1621 tgggtaaagt tggtatgccg ctgcgtgtcg ccgtaaccgg tgcggggcag tctccagcac
     1681 tggatgttac cgttcacgca attggtaaga cccgcagtat cgagcgtatc aacaaagcgc
     1741 tggattttat tgctgaacgc gaaaatcagc agtaattagc gtgtaaaaag ataaacggca
     1801 ggagataata tcctgccgtt ttttatttat gctgattcgt caatatcgag acgggcgagg
     1861 aaattgcgaa ttccttcacg tgacaaaaat tgcgcgactt tttcctgctc aatgtctgtc
     1921 atattatcta tcgcgttgat gatttgtcga tacgccgggg cgcgtgctcc aggagcgtat
     1981 tcagcaattc tctcttcagc ttccatcaac attggtgtgt tatggaaggc tggcgtgttc
     2041 tgaataaact cgcaaacttg cgtatcgacc agaataagtc tggcacgacc gcctttcacg
     2101 ccgggaaatt tttccgtttt ccagcctttt tctctgatcc aacggttaac agtttgttta
     2161 gccacaccga ggcattcagc cagttcttcg gtggtcattt tattgcgtaa tcttttcata
     2221 ttatttgcta tcgcgaatcc ctaagcgttg taacaatccg ataatccctt cccgcagaag
     2281 caaggatgta aactgttttt gttcaactgg cgtcatctct ttcgccagtg tcaccagcaa
     2341 tacttcaagc ggcgcatcac cagaaagggc aggcgcttct ccctgacctt ctggacgttc
     2401 agcattgcga atatattcac gaacctgttc attgacgtga accagtcggg ctttaccacc
     2461 ctggacgcca ggttttggtg acgttgtcca gccttccttg cgtacccatt tattaatggt
     2521 ctggcggcta tagccggtca gtctggcaag ttcatctggt gtcatcctct ccttggacat
     2581 aacaattccc tgaatgtatg tgggtctatt agtagttcat tttataacac cactttacag
     2641 gaaaccgtga catgaataac tactgaatgc gagcggtttt ccaaaattgt tcatttgcct
     2701 gatttttcag cgattgaaac tacacgttgc aatttgccgt tgacacattc gggcggaatt
     2761 catatgatgc cgcccgtcaa ctcgacaagc tttacgtga
//
LOCUS       DAJTYI010000230         2769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYI010000230 DAJTYI010000000
VERSION     DAJTYI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="OO564_003954"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="OO564_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6654853.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..934)
                     /locus_tag="OO564_003955"
                     /pseudo
     CDS             complement(785..934)
                     /locus_tag="OO564_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1010..2707
                     /gene="ospD3"
                     /locus_tag="OO564_003956"
     CDS             1010..2707
                     /gene="ospD3"
                     /locus_tag="OO564_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6654854.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          978 a          457 c          531 g          803 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatggggtt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag
      901 agtaacgggg acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca
      961 acaacactaa gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt
     1021 aaatttaatc ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc
     1081 tgttgagaca atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag
     1141 gagtttttta ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg
     1201 tcgacattta gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta
     1261 tcatcacttt gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa
     1321 ggctattaat gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat
     1381 tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag
     1441 tacatcaaat cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga
     1501 ttttgtcatt cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa
     1561 caaatttaac ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca
     1621 tcagaaatgt tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc
     1681 aacaagcatg tcctccataa tcagatggcc taataattta cttcacccca aagttattta
     1741 tcacgcgatg cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact
     1801 atctgacctt tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt
     1861 ttcaggattg cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga
     1921 actcttggaa acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg
     1981 aatgggagga cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat
     2041 caaaacatac ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa
     2101 aaatttgctg accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt
     2161 tgctcttcaa aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc
     2221 ccctctcctc aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt
     2281 tcccggactt ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga
     2341 tatcttgaat gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga
     2401 ttctaatggt ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat
     2461 tgcttatggg aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact
     2521 ggcagcagaa ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt
     2581 tgaggcaata aaaacttata tgggaataat aaaaaatgaa aatattacac ctgaagaaat
     2641 agcagaacac ttggacaaaa aaaatggaag tgattttcta gaaattatga agaatataaa
     2701 aagctgaata ttatctcgtg gtgatacact gaatttgagg taaccggctc atttaaaccg
     2761 tctggtctg
//
LOCUS       DAJTYI010000231         2730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYI010000231 DAJTYI010000000
VERSION     DAJTYI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..272)
                     /gene="sitD"
                     /locus_tag="OO564_003957"
     CDS             complement(<1..272)
                     /gene="sitD"
                     /locus_tag="OO564_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6654855.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIK"
     gene            complement(269..1126)
                     /gene="sitC"
                     /locus_tag="OO564_003958"
     CDS             complement(269..1126)
                     /gene="sitC"
                     /locus_tag="OO564_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6654856.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1123..1950)
                     /gene="sitB"
                     /locus_tag="OO564_003959"
     CDS             complement(1123..1950)
                     /gene="sitB"
                     /locus_tag="OO564_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6654857.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(1950..>2730)
                     /gene="sitA"
                     /locus_tag="OO564_003960"
     CDS             complement(1950..>2730)
                     /gene="sitA"
                     /locus_tag="OO564_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6654858.1"
                     /translation="VAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNL
                     ELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDA
                     LIKYDPANAQTYQRNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDL
                     GLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHY
                     GGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT          710 a          734 c          662 g          624 t
ORIGIN      
        1 tgcttgattc ggctgttgtc tttcaggtat cccgtcgcca ccgcgcagaa tactccggcg
       61 acgaatgccc ctgtagccag aggtaacccc agaatccagg taagcacgat gccaggaaaa
      121 accgcatgac tcatcgcgtc acccatcagt gcccaacctt tcaggaccag aaagaccgac
      181 agcagcgcac agggtatgga aactaccagt gaaatcagca gtgcgtgact cataaaggtg
      241 aactgcagtg gctccagtaa taatgccatc atgagcgatc ctccagcgct ttatgcgcac
      301 gccgacgatt tgccaaaagt ccatgggtgg gcgcaaatac aaatgctaaa agaaacagca
      361 gtgtctgcgc caccacgatg attccacctg tcgcgccgtc cagaaagtaa ctgacccacg
      421 cgccgaggaa actggttaca ctgccaatag ttacagcaat tatgagcagg cggggaaaac
      481 gatcggttaa caaccatgct gttgcaccgg gagtcaccac cagacagatc accagaaagg
      541 caccaaccgt ttgcagcgcc gcaacggtcg aaaccgacag cagggtgaag aagaggattt
      601 ttaatctgcc cggatgtaat ccaatagcac gggcgtgatt ctcgtcgaaa aaggtcacca
      661 taagatcttt ccatttgaaa aaaagcacga tgatcgagag aatgccaatg atagtgagtt
      721 gcaggatatc cgccggatcg attgccagaa tattgcccag gacgatggtc tgaatgttca
      781 cggatgtcgg gttcaatgac accataaaca gccccaggcc aaaaaaggac gagaagatca
      841 ggccgatgat ggcatcttct tttaagcggg tgcgttggtt aagaaacagc atgctgcccg
      901 ctgccagtcc gccggaaaag aaggctccca gagaaaacgg aagccccagc atatatgccc
      961 ccgcaacacc cgggacaata gagtgcgaca gcgcatcgcc aatcagcgac cagcctttta
     1021 gcatcagata gcatgacagg aaagcgcaga gaccgccgac cattgccgag acccacatcg
     1081 cattgagcat atattcatag ctgaagggtt ccagtagcac gttcatcttg cttccctctg
     1141 caccgctgac ggtcgatgtg ccacaaaagg gcgttcatcg tcagtaataa tgctttcttc
     1201 cgagccgtta agtgtgacat ggcgcagtac gccgctaaaa gccagctcca ggttctcggc
     1261 tgtaaaagtg gtgtctgtcg gcccgcttgc caggacggtg cctttgacca tgaccgtata
     1321 atcgcaaaat gtcgtgactg agccgagatt gtgcgttgag acaagcatcg ttttgccttc
     1381 cgcgcgtaac tccctcaaca ggctgatgat tttcgcttcg gttttcacat caacgccggt
     1441 aaaaggttca tccagcagga tcacatcacc ctgctgtgca atcgctctcg ccagaaagac
     1501 gcgtttcttt tgtccaccgg aaagctcgcc gatttgacga tggcgaaaat ccaccatatc
     1561 gacacgttcc agcgcatcgg tgacaatctg acgatccctc tttttggcta tacgcaacat
     1621 acccatatgc ccatagcggc ccatcatcac cacatcttcc accagtacag gaaatgacca
     1681 gtcaacctct tctgactgag ggacgtaggc aaccaggttt ttctgtagcg cctgtcgcgt
     1741 gggaataccc agaacagata tctttccgct ggtcagacgc acaaatccca taatcgcttt
     1801 aaacaacgtc gattttccgg aaccgtttac cccaaccaga gcggcaatag atccacttgg
     1861 tactgtgaag gatgcatcgc gcagtgctgt gtgcccgtta cgccaggtga cagtaacatc
     1921 attaacgaca atgcctgcag attgcatcat tatttctccc ttttcccggc tttaattccc
     1981 tgtaccagcg tactggtagt aaccttaaga aggtcgatat acgtaggtac cgggccgttt
     2041 tctgtgctca gggaatcgac atagagcaca ccgccgtagt gtgcgccggt ttcacgcgca
     2101 acctgacgcg ctggtttatc ggaaatcgta ctctcgctaa agactgccgg gatatgattt
     2161 ttcttaacta tatcaacaac cttacgtacc tgctgcggtg ttccttgttg atcggcatta
     2221 atcggccaca gataaagctc ttttagcccc aaatcgcgtg cgagataaga aaaagcccct
     2281 tcactggtga ccatccatcg ctgattctca gggagttccg taatctgctt acgcaggggg
     2341 gcaagggttt gggtaatctt ggctttataa gtatcggcat tacgttggta ggtttgtgca
     2401 tttgccggat cgtattttat cagcgcatca cgaatattat cgacgtaaat cagagcatta
     2461 tctggcgaca tccatgcatg gggattaggt ttgccctcat agggtccttc ggtgatccct
     2521 actggcgtca cacccgaaga gacaattact tctggaaccc cattgagatg ctggtaaaag
     2581 cgttggaacc acaattccag attcatacca ttggcgagaa tcagttgtgc cccctgcgca
     2641 cgtttaatat cgccaggggt aggctgatac tcatgaattt ctgcaccagg cttggttatg
     2701 gatgagactt ctgcagcatc tccagccacg
//
LOCUS       DAJTYI010000232         2700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYI010000232 DAJTYI010000000
VERSION     DAJTYI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            514..954
                     /locus_tag="OO564_003961"
     CDS             514..954
                     /locus_tag="OO564_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654859.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1033..>2700)
                     /gene="treA"
                     /locus_tag="OO564_003962"
     CDS             complement(1033..>2700)
                     /gene="treA"
                     /locus_tag="OO564_003962"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6654860.1"
                     /translation="KMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNA
                     KLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEG
                     QSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAE
                     SGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALK
                     QYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIA
                     TAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKM
                     EKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLT
                     AAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVA
                     TEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGF
                     GWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT          649 a          643 c          665 g          743 t
ORIGIN      
        1 gatgataaca gttacggaaa tatgatattt acgctttatt ttgataacga tatctatggg
       61 aacagaatta tcaaagcatc gctgaacggc ggagagttgc agttattaag cacagggtgt
      121 agcaatgata acattctcca ttttattaat gcatttctca ttcgggtgaa atttccgcta
      181 cctggacgca taaaaccgga actctcgcca aaagccgggc actccgaagg aattgacgcg
      241 ttgcgctgct ctgtaacggc atttaccgca agccatttat ttcgccagcg acaatccgca
      301 gagaaccaaa cgcatcagga aacgactgaa cacaataaat actgtattga tcaacatatt
      361 atactggcac ctccagcacg cagtgaggat acactgtatc atcagcgaat cttatcactg
      421 cggagctggt tgaacgactt tctgagcgaa ataagtcggt tcccggtaac aacaacagaa
      481 tcctttttta attattgttt cgttgttttt ggtgtgattc agaaaataaa tattttagac
      541 agtagccagt caggcgttaa aaatggggcg gatagtgagg cggttttggc tgcgcttatt
      601 gaacatttag aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg
      661 cttcagttac gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag
      721 tgtctggatt atgtgattat taatttaact ccagaaagca aaaaaacgtt gatgagtgaa
      781 caccgtaaca atattcaggt agcaattgat gctttatata gccaacgtcg gcgtaaatcg
      841 ccgggtgatg aaagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta
      901 tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaaa
      961 ccatagatat ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg
     1021 agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga
     1081 cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa
     1141 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
     1201 gccaccgccg ccccccgttc cggtggtgct gacatcatat ttttccacca gctttttctc
     1261 ccggtcatag gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca tcgccacctc
     1321 tttttgcccg tagttttgta atccttctgt cgcgacccac tgtaacggtg cccagccatt
     1381 tggcgcatcc cattgttgcc cacttttcac cgacgtggtg ttcaggccgc cgggttgcag
     1441 cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga tctttcgctg ccgcattgac
     1501 gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc actttatgac ttttcaggtc
     1561 gtaatcggca taccagcctt gttgatcgtt ccacaggtat ttttcgatcc ctttttgacg
     1621 ggcattcgcc agcgtttcgt actggtttgc catcgcgtta tctcctgcag ctttgctggc
     1681 gcgggcgagg attttttcca ttttaaacat caggctgttc agatcgaccg gtacgatgct
     1741 ggtggtgcgt aaggtattta actgctgcgg gttgtccatc cagcgcgagc tgaaatccca
     1801 gccagacgca gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt
     1861 gcttttggcg gtggcaatat cttccaccca tgactctggt cgtggcgtat cgcgatcgtc
     1921 ccagtagcgg ttgagaaggg taccatcctg aagttttaca acgcgttttt cctgttgtcc
     1981 ggcttgcagg ttttcaacac cgtccatcca gtaagcatat tctttttgca tttgcggcag
     2041 gtattgcttc aacgcggcat cgccttcatg ctgcgccagt aactctacca tcagggcaaa
     2101 gaagggcggt tgcgagcggc ttaaatagta actgcggttg ccgttgggaa tatgaccgta
     2161 agtgtctatt tcatgagcaa aattggccac catatccgcg actttatccc agtgaccgct
     2221 ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa tatacctcgc gaaaacgtcc
     2281 gcccggcacg acataaggtt ccggcagcgg taacagagaa tcccattttt cggtgttttc
     2341 ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt tcgcgcagtg actgcccctc
     2401 tggcggaaca tatttctcgc cttctttcgg cagggtgaaa ttgacgttaa cgaaatggcg
     2461 cagatcaaat ccgctctggt tttgctgcat ccgataatca gcaaggatca tcagcggatc
     2521 gctgttcggc acggcatcgg caaaggtttt ttggtccgga aaaagtttgg cgttttgcac
     2581 atcattaaac agcggcccta ataaaatatc aggcggctgt ggtgttaccg gtgtttcttc
     2641 tgcctgcacc gatagcgcag cgaaacacaa aaagatacag gctggaatta acgccatttt
//
LOCUS       DAJTYI010000233         2553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYI010000233 DAJTYI010000000
VERSION     DAJTYI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..282)
                     /locus_tag="OO564_003963"
     CDS             complement(<1..282)
                     /locus_tag="OO564_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654861.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGT"
     gene            complement(834..1550)
                     /locus_tag="OO564_003964"
     CDS             complement(834..1550)
                     /locus_tag="OO564_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654862.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            complement(2134..2553)
                     /locus_tag="OO564_003965"
                     /pseudo
     CDS             complement(2134..2553)
                     /locus_tag="OO564_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          782 a          555 c          487 g          729 t
ORIGIN      
        1 ggtcccgcag attccgtata cccgacaccc tgcacgggtt ccgagtatct gtactcccgg
       61 tggtttatca gagtaatagt agtcactgtt acattcgacc cagctctttc ccccgggggg
      121 atgcgggacc actcggaaat acaccccata tggtccgtgc acattaatct ccgcatagca
      181 atgttctgtt gaattacgtg gcatagccag taaagtctct tttgcagcag cgggtaccgc
      241 ataccacata cacattagtg caactatcag cgcagtattc attttcattg tcactttccg
      301 ttactggtgc tgtccgtcac agcacctcat gtcctgcgga cagggcaagc tgaccggcca
      361 taacaacttt cagttccatc agggaagtgg aagctagacg ccggacaatc aaatcccgcc
      421 ggcggtacac cttcttcgca gcagagttgt tcagtcaaca tgaaagtgac agacagtgtc
      481 accacgatgc agcatcagta tgacggtgaa tcgccggtcg tagtttttgt tctttgtttt
      541 atagggagct ttctgcatca ggcatcgttc atcacggggt attggtgcaa tgctcggcat
      601 cactcagtcc ggctggtgat ttgtgatgtt tggcaattta tctgattaca taatccggac
      661 tgagtccccc ctgagtgttt tactgttccg agcacttatt tagtaacttt cttgcatagt
      721 tgtttctggc tttaattgtg ctgccctaag gtttgtttct cagtatgaca gctcaataca
      781 tatacttcga acgcattata tattcattta attcactttg tgggagagga ttattacttt
      841 ctgacataaa catcattgat ttcataaaat cccgactgtt ctgtattaaa tattaagaat
      901 ctgttactgt catctttata tgaactagac aaaacgagcc gtgaatcata acgagcaaga
      961 acataatacc aactattatt ataacaaagc atctgatatt ctttttttaa ttgaggttta
     1021 taccagccaa caagaaagga aaaaagccaa aaataaacca aaaaaccagc cataattaac
     1081 ttaatgcggt gttttctaac aaaagggata tctgaaaaac atgatacaga aaagatccgt
     1141 ccatatatac gaattattat tgaaataacc aatgaactaa taatgcatga aagtgataat
     1201 ggaagtatga aatattgttt gagaattaaa agttcgaata ttacagggaa aaacagtagg
     1261 gtaatagcaa aaaatagtct aaaaaaactc aagtcttgta ttttacttct tttctttata
     1321 agaagaaaaa ataaaacacc aatcccccaa ccggtaaaaa gtatacccat tactatgata
     1381 gcatagagta aacttctggc aacatcatcc aaacctgcac caacaaccca ccatggaaat
     1441 ccatagtaaa aagaggttcc ccagccataa gaataagcac tgacccaccc tacacatccc
     1501 atataggcga tgaataatga agaatttcta agcaatgaat tgtcattcat aagaaaccaa
     1561 ctgataatgt aaaaaatatt cgagataaaa gttgatcttt taatttttat gctaggaaat
     1621 taacaataat atcattgtta atgcatattt atcatatcaa aatactctgc agaatgctac
     1681 tatcaaaacc agacaagtga acaaaacttg gtcaacattt agtaacgcta gttggtaatg
     1741 tcttcaatcg atagaagttt ctggtaatct actggtattc aaacagttag ggctcataca
     1801 gcttgctgga ccttccccat aaccgcccga acgtgaaggt ctgatgtgtt gcaagagagc
     1861 cggatgcggg aaatctgcaa gtccggttcg atgagaggga cgttgttttt acttgttgtt
     1921 gatgaagact ttcattattg aacagaatga taatattgtc taaacaaaac attaaatctc
     1981 ataattcttt gtagaaaacg cttttcttga aattaaaaat aagtagaatc agcagtctca
     2041 ttataataac agacaatgtc atgctctcgc ccgttccggt aataaacgga gcgggctatc
     2101 tttttggaat atttatggcc cacaatccag ttttcataaa tacgattcat tccattcttc
     2161 gttttttctg agcattgcgt tcataatggt cagcaactta cgcatacagg caaccagtgc
     2221 cacttttttg gcgtttccgg ctgcaagtag gcgcatgtaa aacgccttta tcaccagatt
     2281 aaaccggggg cctcaagcgc cgccatgtac agagctgttc ttccccagca caatcaccga
     2341 aaatggttcg tcgaccgcgc agagttcccg agtcccggtt tacctgagca acaccgacaa
     2401 gtgcgctgat ttcacgtctc gagaggctgc tcgattcagg catttccgcc agcaatgctg
     2461 cggcagtcat ggctctaaca cctcagacgt tcagtaagct ctttaaagta actctgaatg
     2521 tgcctgttca tatctgggtc ttccccgatc atg
//
LOCUS       DAJTYI010000234         2518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYI010000234 DAJTYI010000000
VERSION     DAJTYI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..175)
                     /locus_tag="OO564_003966"
     CDS             complement(<1..175)
                     /locus_tag="OO564_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6654863.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLM"
     gene            complement(337..411)
                     /locus_tag="OO564_003967"
     tRNA            complement(337..411)
                     /locus_tag="OO564_003967"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Arg,seq:cct)
     gene            complement(487..1419)
                     /locus_tag="OO564_003968"
     CDS             complement(487..1419)
                     /locus_tag="OO564_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6654864.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1713..2468
                     /gene="mlaA"
                     /locus_tag="OO564_003969"
     CDS             1713..2468
                     /gene="mlaA"
                     /locus_tag="OO564_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6654865.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          601 a          605 c          622 g          690 t
ORIGIN      
        1 tcataagcgg atattttccg acggtcagga ttttctcctt accgtcaatc ttgtagcgaa
       61 gctgccatac ctttttcccg gatacaggga cataaaggta caggccatta ccatcgagaa
      121 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
      181 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
      241 acgatctgac gcgaaccatg acgaactgtg aatatacgga aggcttgata ttcaggggat
      301 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
      361 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      421 tgagtatacc cgctaatgga ctgcggggta agtacgctgc cgctcgattg cttaaaccct
      481 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      541 gcgtgcttct gctttacgct tgttgctcat gtcgttacga atctgtgcat gactcattaa
      601 cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca
      661 gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga tagaggattt caacagaacc
      721 gaccacgata tgggtggtgt cacccagggc aataagccag gtcatcaata taatcaccac
      781 aatctttgcc gcacctgctg caggaaacat ccataccata gtggcgatca gccagccgga
      841 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      901 gacaaatgca tcgcgagttt cttcattgaa gataggcata tattcaaatg cccacgcagc
      961 aataccagtc ccgagaatat tacccagcag cacgactccc cataaccgca taagtaagcc
     1021 aacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     1081 aaataattgc tggcgggcca ttatgacgat aataaaacca aaggtataac cgagattctc
     1141 cagcaagaag ctgcccggca caccttccag ttcgacatga aatatccctt ttgccagtaa
     1201 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg
     1261 ttctagctct ttttcaccat cctggcggat atgctcatga attaccatcg ctcgggaggg
     1321 gagtcggtct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     1381 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1441 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1501 gtagaaaagg ttgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     1561 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1621 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1681 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1741 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1801 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaacgta ttagacccgt
     1861 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gtgcgtaatg
     1921 gtttgagcaa ctttactggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     1981 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     2041 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     2101 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     2161 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt
     2221 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     2281 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     2341 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2401 tcaaaccgca ggaaaatccg aacgcgcaag cgattcagga tgatttaaaa gatatcgatt
     2461 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatc
//
LOCUS       DAJTYI010000235         2502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYI010000235 DAJTYI010000000
VERSION     DAJTYI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..536)
                     /locus_tag="OO564_003970"
     CDS             complement(<1..536)
                     /locus_tag="OO564_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654866.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRL"
     gene            complement(1198..2184)
                     /locus_tag="OO564_003971"
     CDS             complement(1198..2184)
                     /locus_tag="OO564_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654867.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2266..>2502)
                     /locus_tag="OO564_003972"
     CDS             complement(2266..>2502)
                     /locus_tag="OO564_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654868.1"
                     /translation="KRVEAERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERR
                     TYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          666 a          580 c          606 g          650 t
ORIGIN      
        1 agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg cgtagacata
       61 taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg gtctgatact
      121 aatgccatat agccccataa actatccctc agccagacag catcatagtg ggtttctgcc
      181 gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg tttctccgtc
      241 aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc gtctttggta
      301 tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg ataattagga
      361 tacttatctg gatttaacga taaataactt ttatctgtta ctgcccccct ggaattaaga
      421 atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt gccgcacgaa
      481 aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata atccatcaat
      541 cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg tattttacct
      601 gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa aatggggaag
      661 tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag aaaatcagat
      721 acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa gggagccgag
      781 gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta cagtgatggt
      841 tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac aaaaggaata
      901 gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct acggggtaaa
      961 tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac tcatcgtact
     1021 ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac cacgagatag
     1081 actttgtgta ggtccattag gtcctcctct ttccgtaccc atgaactaga ttactagtac
     1141 aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta ttgttagtta
     1201 gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag cgctgccagt
     1261 ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga gaatctcaag
     1321 gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct cgccatcgtg
     1381 acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga cgccacggtc
     1441 tgcaccgttg tagtaggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc
     1501 gaagccctgc ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat
     1561 gccgtagtca gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat
     1621 gatagcgtca gccttagcgg tcaggctacc agtcagggcc ggaagctcta cggtcttcag
     1681 cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga ccacgttatc
     1741 gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag aggacttctg
     1801 gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct caacggtgat
     1861 ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga caggaacgac
     1921 cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt tacgggtcgg
     1981 caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac cgttcagcat
     2041 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagtaggcag
     2101 accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa taccgaactg
     2161 gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat actgtgagtc
     2221 ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca ggctgtccat
     2281 ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc cagcggaatg
     2341 ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct gttcgcggcg
     2401 ctgtagctct ttctgccatt cgtcttcggt catgaacatt tcgcgggtac gctctttctg
     2461 agacgccagc tctttagcga tacgctcagc ctcgacacgc tt
//
LOCUS       DAJTYI010000236         2450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYI010000236 DAJTYI010000000
VERSION     DAJTYI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..583
                     /gene="glpG"
                     /locus_tag="OO564_003973"
     CDS             <1..583
                     /gene="glpG"
                     /locus_tag="OO564_003973"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6654869.1"
                     /translation="PFFAALRERAGPVTWVMMIACVVVFIAMQILGDQEVMLWLAWPF
                     DPTLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLS
                     GYVQQKFSGPWFGGLSGVVYALMGYVWLRGERDPQSGIYLQRGLIIFALIWIVAGWFD
                     LFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK"
     gene            600..1358
                     /locus_tag="OO564_003974"
     CDS             600..1358
                     /locus_tag="OO564_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6654870.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(1340..>2450)
                     /gene="rtcR"
                     /locus_tag="OO564_003975"
     CDS             complement(1340..>2450)
                     /gene="rtcR"
                     /locus_tag="OO564_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6654871.1"
                     /translation="YNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKS
                     RAPILLNGPTGAGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKG
                     AFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSD
                     FQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHAT
                     LTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDE
                     INRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDV
                     SRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT          529 a          663 c          686 g          572 t
ORIGIN      
        1 tcctttcttt gctgccttgc gtgaacgcgc aggtccggtg acctgggtga tgatgatcgc
       61 ctgcgtggtg gtgtttattg ccatgcaaat tctcggcgat caggaagtga tgttatggct
      121 ggcctggcca ttcgatccaa cactgaaatt tgagttctgg cgttacttca cccacgcgtt
      181 aatgcacttc tcgctgatgc atatcctctt taacctgctt tggtggtggt atctcggcgg
      241 cgcggtggaa aaacgcctcg gtagcggtaa gctaattgtc attacgctta tcagcgccct
      301 gttaagcggc tatgtgcagc aaaaattcag cgggccgtgg tttggcgggc tttctggcgt
      361 ggtgtatgcg ctgatgggct acgtctggct acgtggcgaa cgcgatccgc aaagtggcat
      421 ttacctgcaa cgtgggttaa ttatctttgc gctgatctgg attgtcgccg gatggtttga
      481 tttgtttggg atgtcgatgg cgaacggagc acacatcgcc ggattagccg tgggtttagc
      541 gatggctttt gttgattcgc tcaatgcgcg aaaacgaaaa taattccagg gatttataaa
      601 tgaaacaaac acaacgtcac aacggtatta tcgaactggt taaacagcag ggttatgtca
      661 gtaccgagga actggtggag cacttctccg tcagcccgca gactattcgc cgcgacctca
      721 acgagctggc agagcaaaac ctgatcctgc gccatcatgg cggcgcggcg ctgccttcca
      781 gttcggttaa cacgccgtgg cacgatcgta aagccaccca gaccgaagaa aaagagcgca
      841 tcgcccgcaa agtggcggag caaatcccca atggctcgac gctgtttatc gatatcggca
      901 ccacgccgga agcggtggcg cacgcgctgc tcaatcacag caatttgcgc attgtcacca
      961 acaatctcaa cgttgctaac acgttgatgg taaaagaaga tttccgcatt attctcgccg
     1021 gtggcgaatt acgtagccgc gatggcggga tcattggcga agcgacgctc gattttatct
     1081 cccagttccg ccttgatttc ggcattctgg ggatcagcgg catcgacagc gacggctcgc
     1141 tgctggagtt cgattaccac gaagttcgta ccaagcgcgc cattattgag aactcgcgcc
     1201 acgttatgct ggtggttgac cattcaaaat ttggccgtaa tgcgatggtc aatatgggga
     1261 gcatcagcat ggttgatgcc gtctacaccg acgccccgcc gccagtaagc gtgatgcagg
     1321 tgctgacgga ccaccatatt caactggagc tgtgctgatc ctgcacggcc tcccacgtca
     1381 gaccaaaacg cgccaggtat ttgcgtagcc gatccgcgtc attgacgctg gctttgccct
     1441 ggcgcgaaac atcaaaaagc tgtcgtccgg cagcagaaag cgactttgcc tggcggcaga
     1501 tagcgataac gtgttccagt tgcatacggt cgaagagatc gatgttttct gcctctgcgc
     1561 ccagcaacgc cgtaagcgcg gaggggcggc tctcctgcca gttatagcgc agacggttta
     1621 tctcatcttc caccacgtcc agagtgatgc gtccgctagt ggcaaaggtg gccatccgcg
     1681 tgacgctggc agaaagttcg cgaaagttac cgcgccatgt agcctgggga gatgttgcaa
     1741 aggccagcca ggcacgccgc gcttcggtgt taaaacgcac gctgtcacca gtgagtgtgg
     1801 cgtggcgctc cacttcataa tccaggttcg gttcaatatc ttcctggcgc tggcgtagac
     1861 ccggcagggt gaaggtccag agattgatcc gcgcgtacag atcttcgcgg aatttgcctt
     1921 cggcaaccag ctggcgcaaa tcgcgcaccg ttccggcgat aagctgaaaa tcactgctca
     1981 cctggcgatc gctgccaaac gggtaaaagg ttttctcttc aatagctttc agcagcattg
     2041 cctgttcgtc tgcgcccagt tcgccaatct catcaagaaa caacattccg ccgttggcgc
     2101 tgcgtaataa tccttcacga gattcccgcg ccccggtaaa cgcgcctttc acatgaccaa
     2161 acagcgtcga catcgcggta tcgccgcgca gggtggcgca gttcacttcc acaaacgcgc
     2221 cgctaaactg atgccgtgcc tgttttaact cgaagatgcg tcgcgccaga aatgacttac
     2281 ctgcgccggt cgggccgtta agcagaatcg gcgcgcggga tttaatcgcc actttttcga
     2341 tctgctcaat catgcggttg aagtgggggt tacgcgtggc aataccggat ttgaggaaat
     2401 cgagcgtttg ctgccgttcc tcggcaaagc ggctggcgat ggcgttataa
//
LOCUS       DAJTYI010000237         2357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYI010000237 DAJTYI010000000
VERSION     DAJTYI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.241"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            19..972
                     /locus_tag="OO564_003976"
                     /pseudo
     CDS             19..972
                     /locus_tag="OO564_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1013..2008)
                     /gene="pgl"
                     /locus_tag="OO564_003977"
     CDS             complement(1013..2008)
                     /gene="pgl"
                     /locus_tag="OO564_003977"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6654872.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          644 a          586 c          541 g          586 t
ORIGIN      
        1 ccattttccg gagtaataat gaagcatgaa ttatcaagta tgaaggcatt tgtcatactg
       61 gcagagtcca gttcatttaa caatgccgct aaattactca atattacgca acctgcatta
      121 acgcgaagaa taaaaaagat ggaggaagat ttacatattc aactatttga gcgtacaact
      181 cgcaaggtta cgttaacaaa agcaggaaaa aggttgctcc cggaggcgcg ggaattaata
      241 aagaaattcg atgaaacgct ttttaatatt cgtgatatga atgcttatca tcgtggtatg
      301 gtgacattag cgtgtattcc gaccgcagta ttttattttt taccgctggc aatcggtaaa
      361 tttaatgagc tgtatcccaa tattaaagtg cggattctgg aacaaggcac gaataattgc
      421 atggaatcgg tgctgtgtaa cgaatcagac tttggcataa acatgaacaa cgtcacaaat
      481 tcatccattg attttacccc actggtcaac gagccattcg tgctggcctg tcggcgtgac
      541 catccattgg ccaaaaagta gcttgtagaa tggcaggagt tagtgggtta caaaatgatt
      601 ggcgtacgtt cctccagcgg caaccgattg ttgatagaac aacagctagc cgacaagccc
      661 tggaagctgg actggtttta cgaagtgcgt catctttcga cgtcattagg actagtggag
      721 gcaggactgg ggatttcggc gcttcctggt ctggcaatgc cccatgcgcc ctattcttcg
      781 attattggta tcccactggt ggaacccgtt atacgtcgga cattggggat tattcgccgc
      841 aaagatgccg tgctttctcc ggcagctgaa cgcttttttg ccttgctgat taatttgtgg
      901 actgacgata aagacaattt gtggaccaat atcgtcgaac gccagagaca tgcgttacag
      961 gagataagtt aatacaccgg cgcaggagag actgcgccgg ttaaatcagc ggttagtgtg
     1021 cgttaaccac cacccacatt ggtccctgcc cgaccgcata gcggcctttt tcatgcagta
     1081 gcccctgctc gccaacaatt tcgtataccg agatgtggtg agatttttgc ccagcggcaa
     1141 tcagatactt gccgctgtaa tcaacattga agccgcgcgg ctgggtttcc gttggctgga
     1201 agccttcttt actcaacacg ctgccatctt ccgaaacgct gaaaacggta atcagagtgg
     1261 cggtacggtc gcaggcgtat aaatggcgac catccggggt gatatgaata tcagccgccc
     1321 aacgggtgtc ggagaagttt tccggcatca tatccagcgt ctggacacat tcgatattgc
     1381 cgtgcggatc tttcagttcc cagacatcca ctgagctgtt taactcattg acgcaatacg
     1441 catattgttc gtttggatgg aataccatat gacgcgggcc ggccccttca acggtagtca
     1501 cttccgcagg gtcctgcgcc accagatgac catcatcgct gaccgtaaac aggcaaatgc
     1561 gatcttgctt taatgccgga acccacagcg tacggttgtc cggtgagata ttggcggaat
     1621 ggcaaccgtc cagcccctcg actacatcga cgacgcccac tggcaggcca tcttccagac
     1681 gcgttacgct cacgttaccc gcattgtaag aagctacaaa gacaaactgc ccctggtgat
     1741 cggtggaaat atgcgtcgga ctacccggca gcacagactc tgcggcaaag gtcagtgcgc
     1801 catcgtccgg ggcaatacga tacgccagga cgcgaaactc agggcgaaca ccaacataga
     1861 gataacgttt gtccgggctg accaccatcg gctgcacctg ccccggcaca tcgacaacct
     1921 gtgtcagcgt cagtgcgcct tcatgattca ggttccagac gtgaatttgc tggctctcag
     1981 ggctggcgat ataaactgtt tgcttcatga acacacctca aaaatgataa atcccttatc
     2041 tttcatacat ataacaacac ttactggata aaaaacagac aacaaaacac gataaaatac
     2101 aatatcgcac atcaattaaa tcaataagtt agcactccat ttcgaatggc atattactaa
     2161 aatattagtc accgacacga atttgacaat gccacagata ttttcgcgac tattcaggga
     2221 gtatgactct ccccgctttt gtttacctgt atcaacgacc gtaaacacta tgccataaca
     2281 acttctggcc aacttcccat tcgctcaaca atcaactcac ttccggcaca aataaacgca
     2341 ttacgttgta gatcggt
//
LOCUS       DAJTYI010000238         2355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYI010000238 DAJTYI010000000
VERSION     DAJTYI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(89..1594)
                     /gene="glpD"
                     /locus_tag="OO564_003978"
     CDS             complement(89..1594)
                     /gene="glpD"
                     /locus_tag="OO564_003978"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6654873.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            1784..2110
                     /gene="glpE"
                     /locus_tag="OO564_003979"
     CDS             1784..2110
                     /gene="glpE"
                     /locus_tag="OO564_003979"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6654874.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2155..>2355
                     /locus_tag="OO564_003980"
     CDS             2155..>2355
                     /locus_tag="OO564_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="protease responsible for the cleavage between Ser
                     and Asp residues of proteins in regions of high local
                     hydrophilicity; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6654875.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAA"
BASE COUNT          528 a          634 c          626 g          567 t
ORIGIN      
        1 gcctgataag cgtagcgcat caggcagttt tgtgtttgtc atcagtctca ggccagattg
       61 aaatctgacc tgatcacctt acgttaattt acgacgccag cgacaacttc tgctgcgtat
      121 attccaccag ccactgactc acacgagatt gttgatccgc attcagccac atgccttgtt
      181 ttgtgcgacg ccacagggca tcgtcggtgc ggcggaccca ttcgtgatcc accaggtatt
      241 tcagttccgc ttcgtagaac tcatgaccga aatcttcccc gagatcgctt accgttcccg
      301 cattgccgag cagcagctcg ctgttgctgc cgtaagtgcg cgcgtagtga cgtgccagcg
      361 attcagtcag gaacgggtaa cggcggcgca ggcgagcggc gtaatcgtcg cggtcgcctt
      421 caatggcacc acccggcagc acgctctctt tcgtccacgc cgggccaata ccctgataat
      481 acggcgtcag tttttccagc gcatgttctg ccagttttcg gtaggtggtc agcttaccgc
      541 cgaataccga cagcagcggt gctttgccgt tttcatcatg gatatcaagg gtgtaatcac
      601 gggtaatggc ttgcggcgag tcggactcat catcacacag cggacgcaca ccggagtagg
      661 tccagacgat atcgtcacgg cttagctgct ttttaaagtg cgcgttatac actttcagca
      721 ggtaattgat ttcactctct tcaatcttca ctgctttcgg atcacctttg tattccacat
      781 cggtggtgcc gatgatggaa aactcgtcca tccacgggat cacgaacaca atgcgtttat
      841 cttcgttttg cagaatgtag gcctgctttt gggtatgcac gcgcggcacc acaatatggc
      901 tgcctttgat caggcgaatg ccataaggcg aaggcagatg catcccctcg tcgaagaact
      961 gtttcaccca cgggccggtg gcgttaacca ggccgcgcgc ttgccagcta tattttttgc
     1021 cggtatcgat atcttccgct tccacaatcc acaggccgtt ttcgcggcga gcagaggtgg
     1081 cgcgagtccg agtaagcact tcgccgcctt tacgcaccac catctgggcg ttggcgagta
     1141 ccagacgggc gtcgtctacc caacagtcag aatattcgaa tccgcgctta atttccggtt
     1201 ttaacactga atttgcgcca aaacgcaaac caattgatcc cggcaggctg gtgcgtttac
     1261 ccagatgatc gtacataaac agaccaatgc gaatcatcca cgccgggcgc agatgcggac
     1321 gatgtggcag gcgaaaacgc atcgggaagg cgatatgcgg agccattttc agcagcactt
     1381 cacgttcagc cagcgcctcg ctgaccaggc ggaattcata gtgctcaagg tagcgcaggc
     1441 caccgtgaat gagttttgaa ctggcggaag aggtcgcgca agcgagatcc tgcgcctcca
     1501 gcatcagcac ggataaaccg cgtccagcgg cgtctgccgc gataccagca ccattgatgc
     1561 cgccacctat cacaatcaga tctttggttt ccatgctgcc ctcattcact ttcgttaaag
     1621 ctcataaatg ttcgttatcg aacatattag caaagaatcg cgctttgggt aacattgaaa
     1681 aaacatttta gagtgatatg tataacatta tggcgtttat ctgccgcttc gacgtaaact
     1741 gtgcggtaaa tttgcccact tgtttgtaaa gaaagagaga cgcatggatc agttcgaatg
     1801 tattaacgtt gccgacgcgc accagaagtt gcaggaaaaa gaggcggtgc tggtcgatat
     1861 tcgcgatcca cagagtttcg ctatggggca tgcggtgcag gctttccatt taaccaacga
     1921 cacgctgggc gcgtttatgc gtgataacga ctttgacact ccggtgatgg tgatgtgtta
     1981 tcacggcaat agcagcaaag gggcggcgca gtatctgctg caacagggct acgatgtggt
     2041 ctatagcatt gacggcggct ttgaagcctg gcagcgacag tttcccgcag aggtggcgta
     2101 cggcgcgtaa cgctttatac tgtccccttt tgtgtggaat aagcgacagc aacgatgttg
     2161 atgattacct cttttgctaa cccccgcgtg gcgcaggcgt ttgttgatta catggcgacg
     2221 cagggtgtta tcctcacgat tcaacaacat aaccaaagcg atgtctggct ggcggatgag
     2281 tcccaggccg agcgcgtgcg ggcggagctg gcgcgttttc tcgaaaaccc ggcagatccg
     2341 cgttatctgg cggcc
//
LOCUS       DAJTYI010000239         2320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYI010000239 DAJTYI010000000
VERSION     DAJTYI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..132
                     /locus_tag="OO564_003981"
     CDS             <1..132
                     /locus_tag="OO564_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6654876.1"
                     /translation="RICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKERRKEWGIL"
     gene            117..755
                     /locus_tag="OO564_003982"
     CDS             117..755
                     /locus_tag="OO564_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6654877.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            983..1384
                     /locus_tag="OO564_003983"
     CDS             983..1384
                     /locus_tag="OO564_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654878.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1395..1869
                     /locus_tag="OO564_003984"
                     /pseudo
     CDS             1395..1869
                     /locus_tag="OO564_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1958..>2320
                     /locus_tag="OO564_003985"
     CDS             1958..>2320
                     /locus_tag="OO564_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6654879.1"
                     /translation="MVHKSDSDELSALRAENARIIKPLLPPEPATPRAGRPWAEHRKI
                     INGMFWVLCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFID
                     WSATALDGSNIRALKCAAG"
BASE COUNT          685 a          431 c          551 g          653 t
ORIGIN      
        1 cgtatctgta agacactcat aaagaatgat ttttggtgcc ggactctctc cttcagtccg
       61 aacaaacccc ggcactatga acgttacctg cagcgtatga aggaaaggag gaaggaatgg
      121 gggattctgt gacaccggag atacttggca acatgattcg tcagtatttc agtcaggtac
      181 gaactgaaaa agagagcata caggctctga atcatctgcg cagagtgctt catgaggtca
      241 gtccttttgc acaggagcct gtggactgtg tgttgtgggt gaaagcggat gaggtggtgg
      301 ccaacgatta caaccctaat gtcatggcac taggtgagaa aaaactactg aaacagtctc
      361 tggagaaaga tggtttcaca caaccggtgg tggtgtcaga ggagaagaat cattatctgg
      421 tggtggatgg ttttcatcgt cagttactgg ggcgggaagc agatacagga aaacgcctga
      481 ggggctggtt gcctgtagcc tgtattaatc cggagagaaa gggacaggcc gcacgtattg
      541 ccgcgaccat tcgacataac cgggccaggg ggaaacacca gatcacttta atgtctgata
      601 ttgtcaggga tctttcacgt ctgggctgga cgaatgaacg tattggtaca gagctgggaa
      661 tggaccagga tgaagtgctg aggctgaaac agattagcgg gctgacagag ttgtttcagg
      721 aagaggattt cagctcggct tggacggttc gttgatgttg ttaaatatgg gcggtatttc
      781 tgaaagttga tactttgggt agaaaaaatg ataccgaaaa ctatggagga tgtgcttgtc
      841 aggtgactgg acaccatagg tcttaggctt tgataacggg ataaaatgag cggcttttct
      901 cataccatca ggtgtgatga tggcgcagat ttacaaaatc tgattgattc taaagatgta
      961 taagtaaaaa tcaagctttg atatgaaaaa acagatattt attaataata aacctcctgt
     1021 agtcccttac tcagggacgc acgcgaaaat atttaaatac attgaaatcc ctctgccgtt
     1081 tttctacttt atctatacat caggcgaacc attccacata tctgtccaaa atactgttat
     1141 ttatgtatca aaatacaatg gtatatttat taataaatta gttccttttt ctcttttatt
     1201 tgacagggat atatctgttc ttcaaagaag agatatttgt gttgtgcgat ttacgtcaga
     1261 ggaaataagt gaacataacg ttttatttga tcatgacatt gaacgattaa aaaaaatttc
     1321 caaagctcag cttatatctc ccgattatgt tttgattgat ttttctagtg ggggggggag
     1381 atgaacccta tgcaatgtcc tggttaatat ctcagtgtgc tcatcagtgc actgataata
     1441 aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg ctttcatgta
     1501 ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt gtttctgtgt
     1561 ctgaaaaaaa tcgccagaat tgtgatggca aattactccc gtaactggag taattccgag
     1621 ttggccagtg cggttttaat gagtgaatct tctttaaaaa gaagaatgta caaagaggtc
     1681 ggttctataa gtacatttgt acataaaata aagcttacag aagctatcag gaaattacga
     1741 aggacaaata ctcccatttc tgtcatatca tctgagttag gttacagttc accatcgtat
     1801 ttttctaaag ttttctttaa atatttaaag acataccctc aaaacataag gaagaaaaat
     1861 ggtcggtaat gtcaccaatt aggaggactt gcagagccgc ttttttacga tactgagatc
     1921 aaacagacat aatctatgcg gagatttaca gcagggcatg gttcacaaat ctgacagcga
     1981 tgaattatcg gcactgcggg cagaaaatgc tcgaatcatc aagcccctgt tacctcctga
     2041 acccgccaca ccacgggccg gacgcccatg ggctgagcac cgtaaaatca tcaatggcat
     2101 gttctgggtg ttatgttccg gtgcaccatg gcgcgattta cccgaacgat atggggcatg
     2161 gaaaactgtt tataaccgct ttaaccgatg gtcaaagtca ggcgtgatta atattatttt
     2221 caacaggttg ctttcgctac ttgatgcaaa cggctttatt gactggtctg ccaccgcgct
     2281 ggatggcagt aatatccggg cgctgaaatg tgccgccggt
//
LOCUS       DAJTYI010000240         2305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYI010000240 DAJTYI010000000
VERSION     DAJTYI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..334
                     /locus_tag="OO564_003986"
     CDS             <1..334
                     /locus_tag="OO564_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654880.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILI
                     REIKGSSH"
     gene            334..681
                     /gene="tnpB"
                     /locus_tag="OO564_003987"
     CDS             334..681
                     /gene="tnpB"
                     /locus_tag="OO564_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6654881.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            701..1930
                     /locus_tag="OO564_003988"
                     /pseudo
     CDS             701..1930
                     /locus_tag="OO564_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1934..2093
                     /locus_tag="OO564_003989"
                     /pseudo
     CDS             1934..2093
                     /locus_tag="OO564_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          563 a          602 c          627 g          513 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agcagataaa tacaccgtcc gacagtctgt gctgtgagct
      241 ggttctgccg gccggaactc tcaggcttaa aggtaaactg acgccggcgt tattacagat
      301 acttatccgc gaaataaaag ggagcagcca ctgatgatat ctttccctgc aggttcgcgt
      361 atctggctgg ttgcaggtat caccgatatg cgaaatggct ttaacggcct ggcctcaaaa
      421 gttcagaacg tcctgaagga tgacccgttc tccgggcatc tgttcatctt ccgcggacgc
      481 cggggtgacc agataaaagt gttgtgggct gacagtgacg gactgtgcct ctttaccaga
      541 cgcctggagc ggggccgctt cgtctggccg gtcacccgtg acggcaaggt gcaccttact
      601 ccggcccagt tatccatgct tcttgagggc atcgactgga aacacccgaa acgaacggaa
      661 cgcgctggca tccgcatata acccgttgta aagtgaggat atggacacct cactttctca
      721 tgagaatgcc cgcctgcggg cactgttgca gacgcaacag gacaccatcc gccagatggc
      781 gaaatacaac cgcctgctct cacagcgggt ggcggcttat gcttccgaaa tcaaccggct
      841 gaaggcgctg gttgcgaaac tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg
      901 cgcaaaaacc gaacggcaga tactggaagc acaggagcga atcagcgcac ttcaggaaga
      961 aatggcggaa acgctgggtg agcaatatga cccgatactg ccatctcccc tgcgccagtc
     1021 ttcagcccgt aaaccgttac ctgcctcact tccccgtgaa acccgggtca tccgtccgga
     1081 agaggaatgc tgcccggcct gtggtggcga actcagttct ctgggatgtg atgtgtcaga
     1141 gcagctggag cttatcagca gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc
     1201 ctgttgccgg tgtgaccata tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag
     1261 ttatgccgga gcggggcttc tggcccatgt tgtcgctgga aaatatgcag actatctgcc
     1321 gttataccgc cagtcagaaa tataccgtcg tcagggcgtg gagctgagcc gtgccacact
     1381 ggggcgctgg acaggtgctg ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca
     1441 gtatgtgctg atgcccggta aagtccatgc cgatgatatc cccgttccgg tccaggagcc
     1501 gggcagcggt aaaacaagga ctgcccggct gtgggtctac gttcgtgatg accgtaacgc
     1561 cggttcggaa atgcctccgg cggtctggtt cgcgtactca ccggaccgga aaggtatcca
     1621 tccacagaat catctggccg gttacagcgg agtgcttcag gccgatgctt acggtggtta
     1681 ccgggcgttg tacgaatccg gcagaataac ggaagccgca tgtatggctc atgtccggag
     1741 aaaaatccac gatgtgcatg caagagtgcc aaccgacatc accacggaag ccctgcagcg
     1801 tatcggtgaa ctgtatgcca tcgaggcaga agtccgggga tgttcagcag aacagcgtct
     1861 ggcggcaaga aaagccagag ccgcgccact gatgcagtca ctgtatgact ggatacagca
     1921 acagatgaaa attcattcgc tgaaaatgga atgtctccac ggagaacact attatccatc
     1981 gggaaatagt gcgggcaaca gtgtttagtt atatcgagtg cgattacaat cgctggcgac
     2041 ggcaccgttg gtgtggtggt ctcagtccgg aacaatttga aaaccagagc ctcacttacg
     2101 actgtgtcca tattatgtgg gtaggatcaa taaagacgtt ttatatgtgt ctgcttgcag
     2161 ccagaaaatt tataccctgc aggtatcata aacatgcttg acaacttaat tagggccccc
     2221 tcttcctttt atggtgtaat tgtttgattg aatacttccg gggatttcag tatgaaatga
     2281 agtatattta atatacttta caaaa
//
LOCUS       DAJTYI010000241         2233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYI010000241 DAJTYI010000000
VERSION     DAJTYI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..795
                     /locus_tag="OO564_003990"
     CDS             <1..795
                     /locus_tag="OO564_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HCR6654882.1"
                     /translation="ETGSVCLVTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLI
                     TMLARSAVGARLTGYNTWLTGPREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCI
                     RCGACMNTCPAYRHIGGHGYGSIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSV
                     CPVRIPLSKLILRHRRVMAEKGITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASW
                     FINGGKTPLKFGAISDWMEARDLPEADGESFRSWFKKHQAQEKKNG"
     gene            788..1483
                     /locus_tag="OO564_003991"
     CDS             788..1483
                     /locus_tag="OO564_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6654883.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1726..2233)
                     /locus_tag="OO564_003992"
                     /pseudo
     CDS             complement(1726..2233)
                     /locus_tag="OO564_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          555 a          480 c          591 g          607 t
ORIGIN      
        1 gagaccggtt cggtatgcct ggtgaccaat gaaggtaatg cgcgaatgtg taccacgctg
       61 cctaaaacgc atattgcagt gatgggaatg gagcgtattg cccccacgtt tgccgaggta
      121 gatgtattga tcaccatgct ggcgcgcagt gccgttggtg cacgtttgac gggatacaac
      181 acctggctga cagggccgcg cgaagcgggg cacgttgatg gtcctgaaga gtttcatctg
      241 gttattgtcg ataacgggcg ttctgaggtg ctggcctctg aatttcggga tgtgctgcgc
      301 tgtattcgct gcggggcttg tatgaatact tgtccggcat atcgccatat tggcggtcat
      361 ggatatggct ctatttatcc agggccaatt ggtgcggtga tttctccgct acttggcggc
      421 tataaagatt ttaaagattt accctacgcc tgctctttat gcactgcttg tgacagcgtg
      481 tgtccggtgc gtattccgct gtcaaaactg attttgcgtc atcgtcgggt gatggctgaa
      541 aaagggatca ccgcaaaagc agagcaacgg gcgataaaaa tgttcgctta tgccaatagt
      601 catccaggat tgtggaaagt cgggatgatg gccggcgctc atgcggcaag ctggtttatc
      661 aatggcggta aaacaccact caaatttggc gcgattagcg actggatgga agcacgcgat
      721 cttcctgaag ctgacggaga gagtttccgt agttggttta agaaacatca ggcgcaggag
      781 aaaaagaatg gataatcgga gcgaattttt gaataacgtt gctcaggcac tgggtcgccc
      841 gctgcgactt gaaccgcaag cagaagatgc gccgcttaac aactatgcta acgagcggct
      901 tacccaactt aaccaacagc agcgctgtga cgcgtttatt cagtttgcca gcgatgttat
      961 gttgacgcgc tgtgagctga ccagcgaggc gaaggcggca gaagctgcaa tacgtctgtg
     1021 taaagagctg ggagatcagt cggtcgtgat tagcggtgac acgaggctgg aggaattggg
     1081 gattagtgaa cgtttgcagc aggaatgcaa tgccgttgtt tgggatccgg cgaaaggtgc
     1141 cgagaatatc tcgcaggcag agcaggctaa agtgggtgtc gtgtatgctg aatatggttt
     1201 aaccgaatcg ggaggcgtgg ttcttttttc cgccgccgag cgcgggcgtt cattgagcct
     1261 gctcccggaa tcttctcttt ttatcctgcg taaaagcaca atcctgccgc gtgtagcgca
     1321 actcgcagaa aaattgcatc agaaagcgca ggccggtgaa cgaatgccgt catgtattaa
     1381 catcatcagt ggtcccagct caacggcgga tattgagctt atcaaagtcg tcggggttca
     1441 tggtccggtg aaagcggtgt atctgattat tgaggattgc tgagagagat atgagatctg
     1501 aacataaacc tgaagaaggc atacgaggtt gaaaatagcg ttaaggacaa cctgacttaa
     1561 ccccgttccg tcatggttat gctgctcttc tgtaaatatc caggcattag taatgccagg
     1621 atatttacag tgaaaaagac aggcatccat tcctgaaaac gggttccctt cccggtcatt
     1681 gtcacttcag ctttatacag gcactctcta tcagaatgct gtttattact gcccagagtt
     1741 ttgttggtat tcatctacca tttttttgac aaggcaaaac attacagaaa ttacaatgct
     1801 tagaaaaaaa tcccagagct aggcaaatgg cagcagggta tttatttttt atttctggtg
     1861 agcttatagt tacacagcca tcaccgttcg gatacaatga ccagttcctg gtcaggtata
     1921 tcatttttgt ataaagctca ctgttaattg ctgaaggcgt ttgtatctca tcgagaactt
     1981 gcctgccatt atggtattct gacgcatacc atttgataat agacgcctgc acgcgagata
     2041 tttcctcttc agcagttggt aaatctctct tatctcctga ccattccagc cttatttctc
     2101 tgttcttgat atctgggctg gtctgaaaaa atgtagtaat tcgctgaata acatcttcag
     2161 tcattgtggt ctgacaggca aaattattat tgctatcaca gctaacaagt atacctggcg
     2221 ttgtctggtt tat
//
LOCUS       DAJTYI010000242         2154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYI010000242 DAJTYI010000000
VERSION     DAJTYI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            70..630
                     /locus_tag="OO564_003993"
                     /pseudo
     CDS             70..630
                     /locus_tag="OO564_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            745..1086
                     /locus_tag="OO564_003994"
                     /pseudo
     CDS             745..1086
                     /locus_tag="OO564_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1069..1906
                     /locus_tag="OO564_003995"
                     /pseudo
     CDS             1069..1906
                     /locus_tag="OO564_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1956..>2154)
                     /locus_tag="OO564_003996"
     CDS             complement(1956..>2154)
                     /locus_tag="OO564_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6654884.1"
                     /translation="NKALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALAR
                     RRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          528 a          526 c          651 g          449 t
ORIGIN      
        1 taaaatatta tcagatgact gcttagaaaa agaacaaatg gtggtgtccg ctattgccag
       61 tacacctcag ttctgggcct tgcccgtagc agttattttt atcaccgggc tgcactgaaa
      121 gccggtgata aatacgccac gatacgtaag atgctgacag atatcacgga gttccagctc
      181 cctgcaggta aagtctggct gtcaccagtg gtggactgct tcgatggaaa agttgtgagc
      241 tggtctctca gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc
      301 gaaacgttaa atgctggcga acgaccggtg atacacagtg acagaggtgg gcattatcgc
      361 tggccaggct ggctggaaag agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa
      421 ggatgttcac ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg
      481 tattatgggc gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca agtggatgct
      541 tacatcagat ggtataacga gcggcgtata aaattatcgc tgggtgccgt cagccccaaa
      601 atgtaccgcc aacaatgcgg gctggaatga taaagcagtc caggaaatcg tccgcatccc
      661 cccttcacca tttggattgt ccgctccacc caacatgttg tttccttaag gttcctacac
      721 cacaaaggaa actctcatgc tgcgcagtgg atggctgacg ttgccgacag gcgggaactt
      781 cgccagttca ggcagacacc ggaacagcgc ttcacgcagg aacaggagca tctgcagccg
      841 ctgctgggta cggacttcga tatccgtcat gtctcctggg atggctatat cgaggttggc
      901 gggaaccgtt acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat acggatctcg
      961 ctggatgatg aactgcggat ctacagtaat gagcaacagg tagcatcgca ccgtctctgt
     1021 tcggctgcat acggctggca gaccgtgccg gagcatcacg ctccactctg gcagcaggct
     1081 tcggagcgca tggccgaacg gctgggggag atacagaaac gggtaatcac tgtctgtgac
     1141 cgggaagcgg atatctggca ttacctgtat tataaagtca gccacggaca gcgtggtgcg
     1201 tgctgcacag aatcgccggc tggaagaggc acccggcaag ctcttcgaac tgccggaagt
     1261 cctggcaacc gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg
     1321 tcaggcccgg atgttcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg
     1381 ccaggcgctc ccgctcacgt atgtctgttg ccgggagcag gcagaggacg gtgcctgctg
     1441 gcatctgctg acgtcaggaa aagcggcgag tgccgccgat gcacgacgta ttgtcagcca
     1501 ttacgagcga cgctggctga cagaggaata ccacaaggcg tggaaaagtg gtggtacatg
     1561 gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat ccaggcgttt
     1621 atagcggtac gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac
     1681 agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa gctggaagga
     1741 aagccgcttc cggtgcaggc accgaccctg aaatgggcct ggggcgatgg catgacagca
     1801 aacgcacagg tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac ttcaggatat
     1861 ggttgagggt tacctggtga tgaagtctct tgatcaggag ctctgatcaa gagacagcag
     1921 cttagggggg ggcccctatt aagttgtcaa gcatgttatg acccctgcgg ggtataaaaa
     1981 gtcccgtcgc gcatcatggc gaacagaacg tcgcagcgtc gtctcgccag ggcgataagc
     2041 gcctgattgt gtcgttttcc ctgactcatt ttgcgggtgt agtaagccct ggagagcgga
     2101 tccctgagcg cggcgaaggc cgacaggaac aacgcccgtt tgagagcttt atta
//
LOCUS       DAJTYI010000243         2085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYI010000243 DAJTYI010000000
VERSION     DAJTYI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..259
                     /locus_tag="OO564_003997"
                     /pseudo
     CDS             1..259
                     /locus_tag="OO564_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            428..1792
                     /locus_tag="OO564_003998"
     CDS             428..1792
                     /locus_tag="OO564_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654885.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            1842..2051
                     /locus_tag="OO564_003999"
                     /pseudo
     CDS             1842..2051
                     /locus_tag="OO564_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          549 a          392 c          473 g          671 t
ORIGIN      
        1 gtacggaaat aaaaccagtc gaaatgccgg taacgacgat tactcctgtc tctgatgccg
       61 gtggactaca agattttatt tactggcgtc ctgatgcgga caggactggt atggaacctg
      121 tttatgtgat acttaatgat cctctggatt ccgggcgatt tacgcgtaaa caactaaata
      181 aaaatatctt aaacatgcaa gtaattttgg tattctcgat acaagaataa atagtagtaa
      241 ctactatagt tgcaaataat gttaaaaaca tttctatgat catctttaca ttttttatga
      301 ttgacaattg tttttggtta aaaaaagaca actaatgagt gtgataatgc aatactttga
      361 gtaattatat aatgataagt aaacaggttt gcgacaattg gttatcagta ttacagaagg
      421 attaaatatg tcattattca cagaacctaa ggaaattgaa agattaccct ctgaagagat
      481 tgaacgatta tatcccgtcc tgcgatacag ggtctttatc agcattttcc tcggatatat
      541 ggggtattat tttgtccgaa acaccaccag tgtgttatcc ggtgtgctgc acatgtctgc
      601 aaccgagatc ggcattatct cctgtgccgg ttttttatcc tatggtatat caaagtttgt
      661 ttccggactt atttctgaca gaagtaattc aaaagtattt ttgtctcttg gtttattcct
      721 gtccggactg gtaaattttc tgataggata tattcccggg atcatcacat ccgttacgtt
      781 attttcaaca atgtatttac ttaacggctg gatccaaggt atgggatatc ctcctggtgc
      841 caaaacactg gtgttctggt atgaacatcg ggaaaggatc acttgggcca cactgtggaa
      901 tttatcgcac aatgtcgggg gagctctggc ccccgttctg attggtttca gttttggttt
      961 tttcggcgac agtgctctgg agcatgcccg ggccgcattt atcttcccag gagtactgtg
     1021 tatggcaatg agtgtgctga tttactttat tcaggtggac agacctgtat cagtggggct
     1081 tcctcccatt gaagaatgga aaggtaacgt cgtttcacac ccggctaaag gccgtgaaca
     1141 aggtccgaga ctctccattc ctgatatcat cagaaaacat atcatccgaa ataataaact
     1201 catttactgc tgtatctatg gttcgtttgt ttatattctg agatacggta ttgtttcctg
     1261 ggctcctaag tttttaagtg acagtctgga tgtcggtggt aaggatatgg gaaaactggc
     1321 atcaatgggg gggttttctg tttttgaaat cggtggtgtg gctggtatgc ttcttgccgg
     1381 atacctgtct gtcagactgt tcagaaactc aaaaccatta acaaatactt tatttctggc
     1441 acttaccatt attttattaa tcgcgtactg gtacgtgcca tcaggcaatg aatatctctg
     1501 gctaaactat acaatcctta ttctgttggg gctggcagtg tatggtccgg tgatgtttat
     1561 tggtctttat tcaatggagc ttgttcctaa agaagctgcc ggagccgcat cagggctgtc
     1621 aggcacattt tcctatatct tcggctcaat tgttgcgaca ctgggaatgg gacttgttgt
     1681 cgattatctg ggatgggggg ccacatttat cgtactgata ctgtctgctg tttttgccat
     1741 cattttcaca ctgatgagtc gtgaacgctc actggagttc gaaaaagagt aattttctca
     1801 ggaaaaacat aaaattactg actaaatatt tattttttga atcgcagggc attccggata
     1861 aggttgatat cgtgtcacgg ctgaagacct atggatacag tatttctggt gtggagacgg
     1921 atgacggata taaggcgctt gtccgcgctt ttcagcttca tttccggcag aaaaattatg
     1981 acggcataat ggatgctgaa accgctgcca tcctgtatgc attgctggag aagtatttcc
     2041 cgggtaaata aggagtgagt cgtcagagct gcgatatatc ccctt
//
LOCUS       DAJTYI010000244         2064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYI010000244 DAJTYI010000000
VERSION     DAJTYI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..444)
                     /locus_tag="OO564_004000"
     CDS             complement(<1..444)
                     /locus_tag="OO564_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6654886.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDI"
     gene            complement(419..1594)
                     /gene="mdtL"
                     /locus_tag="OO564_004001"
     CDS             complement(419..1594)
                     /gene="mdtL"
                     /locus_tag="OO564_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6654887.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1726..>2064)
                     /locus_tag="OO564_004002"
     CDS             complement(1726..>2064)
                     /locus_tag="OO564_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6654888.1"
                     /translation="LGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIY
                     GIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFG
                     NVFNVLPKFG"
BASE COUNT          584 a          552 c          532 g          396 t
ORIGIN      
        1 aatatccact tcaccacgag taatggcatc taaggaatcg taatcccagt tacgtagttt
       61 gatggtcgcc tgcgggtaac gttggtagat ccgtttcgac agcgcattaa gcatgatcat
      121 catcagcggc gattccgccg ccaactcaaa cttcaggccg cgcggtgtct ggtggtgcgg
      181 tttatccagc agcagattgc tcatttgcat ccattccgcc agattttgct ccatgctgac
      241 cattagctgt gtgggcgaca gccccagcgg tgagttcaca aagagcgggt cgtcaaacca
      301 cgctcttaac tttgccagcg acttactcac cgctgaaggg gtcacgttca tccgcttcgc
      361 cgctttggtc acgctgcgct cctgcatcag cagttgcaga cagagcagca gattgagatc
      421 aagcgtggtg atggatttct tcatgagcgg caacggggcg tccaggcgcg acgaacataa
      481 tcagcaacag gctcactatg ctacaggcaa tcagaatccc gatcagcata ttccatgcgc
      541 cgataccaac caccgcggcc agccaaatcc acagtgacga accgcaaacc tgcgcaatac
      601 ctaaggtcga gctggctacg cccgcgcgta atgaaaatgg tcctaacgcc tgactcatcg
      661 ctacgccaaa acctaccgag aaaccggcgc aaatcagcgt gataccaaac agagaaaccg
      721 catgggaagg tgaaacggca agggtgatcc ccgccgcaag gaataacacc tgcgaggtga
      781 tcatcaacgt acgtggctta aaaattccca gcgcaaatgg cgtcgagaat gaaaaggtca
      841 tgctgacgcc agcggtcagc gccataatgg tggcgtattc tccgcgctca aaccccatga
      901 tttccatcag caataccggc gatgtattga cgaaagtgag gatcaccgaa acgctgaggg
      961 tggtgataac aacacggctg aggaaaaaac gattaagcag cgactcgcta ttttctcgtg
     1021 gtttgtccga agcagcgggg gacgctgggc gcgtttcttt taaaataaac aaagacaaca
     1081 tcagtaccgc gatgcccatc attgccatcg cccagaacag actctgccac gggaatttaa
     1141 gcataatcag atgtccgagc actggcgcta acaccggaat gatgcaggta ataccgttga
     1201 gtaatgacag cactttagcc cgacgtcgat catccagcgt gtcgcgcaaa atggcgaacg
     1261 ccaccacgta acaacagcct gcgcccaacc cctgtagaaa tcggcctgca agaaataacg
     1321 tgctggtttc agccagtgaa cagaacaccg aggcaataat aaatagcgcc gcgccgggta
     1381 tggcgaccgg ctttctccct gaacgatcgg ccactttacc ggcaaatagc atcgcagctg
     1441 ccatccccgc cagatatacg gagaacgcaa tatgcaactg cgcttcgctg gcattgagat
     1501 cggcggcgat gcgcggtaaa ccaacgaggt acatatcaat cccggcggga taaagtaaaa
     1561 ccagggcaaa actacaaatc aaaaagcggg acatgggcta aagcgtcctg atagtaaggt
     1621 gacgcaaagc atacgtggtg aaggaggata aggcgagttg ccatttggac ataagtgatt
     1681 tcctgagagg caagaagcca gcgaatggct ggcttcttga aggatttagc caaatttagg
     1741 taacacgtta aagacgttgc cgaaccagca caaaatcacg gttataccaa agagaatgac
     1801 aatcgccgga ataagattgc cgccccagac cgtgaacatc tgattgggaa acttcttgcg
     1861 agctttgatt gcaagcactg cgggaataat gaccgcccag atggtggcgc acagcccggc
     1921 accgccgatc ccgtaaataa agccgttcgg gaagatcaga tacaacaacg caggtggcag
     1981 gaaggttaac agcacggttt tgaaacgccc gccgtgggag ttatcaatct taaacaggtc
     2041 cgccagataa tcgaacaacc ccag
//
LOCUS       DAJTYI010000245         2061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYI010000245 DAJTYI010000000
VERSION     DAJTYI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..261)
                     /gene="ospE2"
                     /locus_tag="OO564_004003"
     CDS             complement(<1..261)
                     /gene="ospE2"
                     /locus_tag="OO564_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR6654889.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSD"
     gene            complement(443..844)
                     /locus_tag="OO564_004004"
                     /pseudo
     CDS             complement(443..844)
                     /locus_tag="OO564_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1273..2061
                     /gene="virF"
                     /locus_tag="OO564_004005"
     CDS             1273..2061
                     /gene="virF"
                     /locus_tag="OO564_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6654890.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          632 a          367 c          384 g          678 t
ORIGIN      
        1 atcagaatac cgttggcttt gtttttcatc aattccggct tcatttatct ttgtcaacca
       61 ttgcatttct gtagcagtga atttcaacgt tttcgtccgg ttggcagcag gtatttgtaa
      121 ttcgtatata gctgcttttt tattgaaaac agtatatcca tctgtagtaa cagtagaaga
      181 taataataca ggatttgata cttcaaggat gatattttct tgtttttgtg gaaggcaagg
      241 gaatatagtt tgtgtaagca tattgcttcc ttatgttata ttttagtaaa gaatttattt
      301 tttacattat agtcggctaa atgaagttta ctgtaaaaaa acgatacttt tttcgtaagc
      361 gatgagatgt ggagccagaa aaatggctgc gttacgtcat tgaactgtcc ttgacccacg
      421 atattcagac agtcgttgta tcttaagttg acattacccg ctgacgtcga tattcccttg
      481 gtgagagata acccaacgca ctatgcggat ggttttcatt gtaatgctcg atcgccactg
      541 caaggttatg caatgccgtt cttggtttcg gcatgaacgc gatgcagtct tccttcatcg
      601 ttttcacgaa ccgttctgct attccattac tctgagggct actgactgcc gtcgtgcagg
      661 gctccagatt caactctctg gcgaactgcc gtgtttcatg agccgtataa gccgaaccgt
      721 tatcggccac tggattggcc gttccggtcc tttatcgcca tatcactcct gacatgacat
      781 cctgcaccgt tgctttgtca taatctcccg tgcctgctgc ccagtctatg gcttcacgat
      841 cgcaatatgg aacgtaacgc tgtcatcggg agaatcgtca atgcggcatt cggttaacgc
      901 ttaccaatat caacaccaac cggatttgtg gagcctccag tctgaagggg ctttatgcgt
      961 tccgtatagg atattatgat gctggagttt tgcgaagctc cactcctttc cgaacattta
     1021 cacagatatt gcctaagaaa agtgaaacca taatatggtt atagtccctt tcagtgcaaa
     1081 tacttagctt gttgcacaga gaaatagaag ctgcataagc tctttcttca aaaaatgtaa
     1141 ataaagttaa atataggaaa aattacttaa tctatcttaa taacggaaag attttgtata
     1201 caatcactgt tacagaaatt tcttagttac tctgtaaaca ctaaatatag tttggtatat
     1261 tctgttgaat ttatgatgga tatgggacat aaaaacaaaa tagatataaa ggttcgcttg
     1321 cataactata ttattttata tgcaaaaagg tgttcaatga cggttagctc aggcaatgaa
     1381 actttgacta tcgatgaagg gcaaattgct tttatagagc gaaatataca aataaacgtc
     1441 tccataaaaa aatctgatag cattaatcca tttgagatta taagccttga cagaaattta
     1501 ttattaagca ttattagaat aatggaacca atttattcat ttcaacactc ctattctgag
     1561 gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg aggaggaggt ttctatcgat
     1621 ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa agatctatag tttagcttgc
     1681 cttttatcag ctgtttctga tgaggaagct ttatatactt cgatatcgat agcttcttct
     1741 cttagttttt ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa gagatggcgt
     1801 ctttctgata tttcaaataa cttgaattta tcagaaatag ctgttagaaa acgattggag
     1861 agtgaaaaat taacatttca acaaatcctt cttgatattc gcatgcatca tgcagcaaag
     1921 cttttattga atagtcaaag ctatattaat gatgtatcaa gacttatcgg aatatcaagc
     1981 ccatcttatt ttataaggaa atttaatgaa tattatggta taactccaaa gaaattttac
     2041 ttatatcata aaaaatttta a
//
LOCUS       DAJTYI010000246         2011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYI010000246 DAJTYI010000000
VERSION     DAJTYI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            235..492
                     /locus_tag="OO564_004006"
     CDS             235..492
                     /locus_tag="OO564_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6654891.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLESQGSLCPICRQRITHETGWN
                     VHHKVKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            635..856
                     /locus_tag="OO564_004007"
                     /pseudo
     CDS             635..856
                     /locus_tag="OO564_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(877..939)
                     /locus_tag="OO564_004008"
                     /pseudo
     CDS             complement(877..939)
                     /locus_tag="OO564_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1389..>2011
                     /gene="ospC1"
                     /locus_tag="OO564_004009"
     CDS             1389..>2011
                     /gene="ospC1"
                     /locus_tag="OO564_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6654892.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGA"
BASE COUNT          674 a          405 c          381 g          551 t
ORIGIN      
        1 gtgaaactgg gctgcattcg actcttttat caacgcttac gtaattaagg gacacagcct
       61 agtctccagg attcccgggg cggttcaatc aaagcgcaga acttctgcgt cttcttcgtc
      121 aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga agtttgcttc tttgagaggc
      181 gggtttacgt ttacggggtt tagctgaacg ggccatataa ccacctgaaa gacaatgaca
      241 ttgcctgttt ttataacggt aattgcagac catgacaaac cgcagccgtc aggctgccta
      301 ctcgagtcac aaggctcttt gtgtccgata tgccggcagc gcattaccca tgaaactggc
      361 tggaacgtac accataaggt gaagaaagtt atggggggcg gtgaagaatt atctaacctt
      421 gtcttgttac atccaaattg ccatcgacag ttacacagtt gtgaagccgg ctctctcaaa
      481 aagggcttat aaaggcttga gcggtgtacc gggaaaccgg tatgcaccgt tcttagggga
      541 gggcagtcca gcaatgaact gtccttaccc gacaaacatc caaaacgaac ggaacgtgct
      601 ggaatccgca tataacccgt tgtaaagtga ggaaatggac acctcacttg ctcatgagaa
      661 cgcccgcctg cgggcactgt tgcagacgca acaggacact atccaccaga tggctgaata
      721 caaccgcctg ctctcacagc ggatggcggc ttatgcttcc gaaatcaacc gactgaaggc
      781 gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa
      841 aaccgaacgg cagattccat tttcacgggt gatctacgcc acacaggctc tgggttgcag
      901 tgactgctcc attaaggcga ttttgaactc ataagggaag taacaaaaaa actgcaaaag
      961 tacaataatt atatatggcc aaactaacct ctattaaaaa acatcagttt ttcacactta
     1021 gttgagcttc atgaaaaaat catcgaggat ttcattgcag aatacacatt ccattaactg
     1081 gaagcactcc ataagaggaa tcagagaaag tatcgttttt ttatagtaaa attcattgct
     1141 gttcaataaa attgatatag ttattaaatt atgaaaataa tttcacaaaa aagattaact
     1201 tgagttctta agcatcggat tttagatatt ttgaaatatc tggcatcaca taacatcaaa
     1261 agtatataat acccttatct aattacatgc agtatattgt atatatactc aaaaattgta
     1321 attcataccc tgctatatat tgtagggaaa gatattaatt aaaactgttt tcatataagg
     1381 ttcattttat gaatatatca gaaacactga actcagcaaa tacccaatgc aatatagatt
     1441 ctatggataa cagattacat acattgtttc caaaagtgac atcagtgcga aacgctgcac
     1501 aacaaactat gccagatgaa aaaaatttaa aagatagtgc aaatattatt aaagatttct
     1561 ttaggaaaac tatagcagca cagagttata gtagaatgtt ctctcaaggc tctaacttta
     1621 aatctttaaa tatagcaatt gatgcaccat cagacgctaa agcctcattt aaggctattg
     1681 agcaccttga cagattatcg aagcattata tatctgaaat aagggaaaaa cttcatcctc
     1741 tttctgcaga ggaactcaat ttgctttcgc taattattaa ttctgattta atcttcagac
     1801 atcaaagtaa ttctgatttg tctgataaaa ttttaaacat taagtcattc aataaaattc
     1861 agtctgaagg aatatgcaca aaacgaaaca catacgctga tgatataaaa aaaatagcta
     1921 atcatgactt tgtgtttttt ggcgttgaaa tctctaacca tcagaaaaaa caccccctga
     1981 atacaaaaca tcacactgtt gattttggtg c
//
LOCUS       DAJTYI010000247         1874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYI010000247 DAJTYI010000000
VERSION     DAJTYI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..1045
                     /locus_tag="OO564_004010"
     CDS             <1..1045
                     /locus_tag="OO564_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6654893.1"
                     /translation="GTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNSVAQMYQQ
                     NPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQN
                     LSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAVN
                     NATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALVN
                     SQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAEW
                     MSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQVG
                     SYTSKSGIQFTVE"
     gene            1045..>1874
                     /locus_tag="OO564_004011"
     CDS             1045..>1874
                     /locus_tag="OO564_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6654894.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     G"
BASE COUNT          536 a          417 c          510 g          411 t
ORIGIN      
        1 cggcacctta gcggctggcg cacgccttgc ggcctcgtct ccagaagcaa tgcaatcatg
       61 gctgcaaaac aacgccaatg agctggcgcg cgtcggtgtt gaccctaaca gcgttgctca
      121 gatgtatcag cagaatcctt caggatttgg tgagtttgtt gatcaccttg gaatggctgc
      181 tcttggtccg attgattact tcaatgttca ggacaagatg gctggtcgtg aaattgaccg
      241 aggcaggctg gcagagacaa tccgcagcaa tcaggctgga gaagcacttc aggcgagagg
      301 gcaaaacctt tcctatcagt cagcaatgac tgggcacaat atcgcagcac aacgcttggc
      361 tctggatcag caagagttcg ggtttaagat gcagcaagcg caggaaaagg ctcagcagtt
      421 gattagcgaa gcacctaagc tgtcagtaaa catggaaaaa ggcatcgaga cggctgtaaa
      481 caatgctaca gcatcatcaa actcagccaa ttctatgagt gcgcttgctc aacagttcag
      541 agcagaaaaa ccaacgacag gtttgttcgg taacgcacag aacatgttcg caaaacttac
      601 cggaagcgat acaacattgc gtgatttgcg cattcgccaa aatgcccttg ttaacagtca
      661 ggttcttaaa ttcctacctc ccggcccagc aacggataaa gacgttgaga tcgttcgaca
      721 gggtgcgcca actgacatgg ataaccctga gacggtcgca agatggcttg atgcaatggc
      781 aaaccttgag cgacgaaacg cgcagtttaa tgagtttaaa gccgagtgga tgagcgcgaa
      841 tggcaaccct ggacaatcgc gtaatggcgg tcagatattg gggttggatg ttaaaaaagg
      901 tgaatcattg gggagtgccg ttaagcggta tatgtcaatg aatactgacg cagcgccagc
      961 acaagattcg acaccttcag gagaaccacg gaatcaggtt ggatcatata cctcaaaatc
     1021 aggcattcaa tttacggtgg aatgatgaaa gtaactgcaa acggtaagac atttaccttt
     1081 cctgatggta cgagcaccga agatattggc accgccattg atgagtattt tgctggtcag
     1141 gctgttcagc aacaaacagt taatcaggcc aataatgcac caacacggga agaaccatca
     1201 ttgatgcaac aagctggcga ttggctcact ggtggtcaaa gtgcagggca aattgcagaa
     1261 caggctggtc gtggtctggt aaacatacca tttgacgtat tgcagggcgg cgcaagtctg
     1321 attaatgcaa tcagtcaggg gcttggtgga cccaaggttt tggatgatgt ttatcgtcca
     1381 gtagacagac cgacagaccc ctacgcgcaa gccggtgaaa caattggtgg gtatttagtt
     1441 ccaggagttg gaacggcagg aagcatggct attggatcac tggcagaggc cgcaaatcag
     1501 aaaggcgatt tcgcacaaaa tgcagctaaa aatgccggag ttaaccttgc cgctcagggt
     1561 gttctttccg cagcagcaaa gggaataggg cgtggaataa cggctataaa aggtgatatt
     1621 gcgccagaag tggcgaagaa aattgccaca tcagaatcga tgggcgtgac accaatgaca
     1681 tctgatgtta tcccgccgaa aaatgctttc actcgcggcc taactcagga tgccgagggg
     1741 gctttgctcg ggacaggctc aaagcgagcg gagcaatatg caacgcgtag taagctggta
     1801 agtaattatt ttgaccgttt tggtgagtac aaccctgatg atgtggtgaa atctcttacc
     1861 accacgttaa gggg
//
LOCUS       DAJTYI010000248         1860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYI010000248 DAJTYI010000000
VERSION     DAJTYI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..726
                     /gene="mppA"
                     /locus_tag="OO564_004012"
     CDS             <1..726
                     /gene="mppA"
                     /locus_tag="OO564_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6654895.1"
                     /translation="FNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFIPD
                     VTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAV
                     ASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSMHSG
                     NISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPW
                     LKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(777..1808)
                     /gene="ynaI"
                     /locus_tag="OO564_004013"
     CDS             complement(777..1808)
                     /gene="ynaI"
                     /locus_tag="OO564_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6654896.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          564 a          414 c          415 g          467 t
ORIGIN      
        1 tttaacacgc aaaaagggcc gacggcagat cagcgcgttc gcctggcatt aagtatgacg
       61 atagatcgcc gcctgatgac cgaaaaagtg ttggggacgg gcgaaaagcc agcgtggcat
      121 tttataccag atgttaccgc cggatttacg ccggaacctt cgccgtttga acaaatgagt
      181 caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg cagctggtta tggtccgcaa
      241 aaaccgctga agctgacgct tttgtataac acttcagaaa accatcaaaa aattgcgatt
      301 gctgtagcat cgatgtggaa aaagaacctt ggcgtagatg ttaaattgca aaatcaggaa
      361 tggaaaacct atatcgatag ccgtaacacc ggcaattttg atgttatccg cgcctcatgg
      421 gtgggggatt ataatgaacc ctccactttc ctgacattat taacgtcaat gcattcagga
      481 aatatttcac gctttaacaa tccggcatac gacaaagttc tggcccaggc atcgacggaa
      541 aataccgtta aagcgcgtaa tgccgattac aacgcggcag aaaaaatcct catggagcaa
      601 gcaccgattg caccaattta tcaatatacc aatggacgat taatcaaacc gtggctgaaa
      661 ggttatccca ttaataaccc tgaagatgta gcgtacagtc ggactatgta tattgtgaag
      721 cattgatgtg atgggaactg gcgttaccct tgtggataac gccagtgatg tctgatttag
      781 cgaccctgtt ccggcggtgt aatgttatcc atatacagcg tctggctggg gaaggcaaag
      841 tccgcgccgt gtgactgtac aatatcgata atcttcaaat aaacgtcttg ctgtgcagca
      901 agccactcag cccataccgt ggttttggta aagcaataaa ccataatatt caatgacgag
      961 tcagcaaact ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc cgggtgattt
     1021 ttcagcatct cacgtacggc ttcgacaata acgcccactt ttgccgcatc ctcataacgt
     1081 aaaccaatgg tcgtggtaat gcggcggttg gtcattcgtc ctgggttttc tacgctgatc
     1141 gacgaaaaca gcgagttcgg tacgtacaat ggacgattat caaaggtcgt aattttggta
     1201 attcgccagc caatttccgc tactgtacct tcgatatttc tgtccggtga acggatccag
     1261 tcgccaatac tgaaaggacg gtcgaaatag agcatgatcc cggaaaagaa attactcaga
     1321 atatctttac cggccatacc gacagccaga ccaccaatac caccaaaggt cagcaagcca
     1381 gaaaggctca tgccgaaatg ttcgccataa agcagaacaa gcactacaat aatggtgatt
     1441 ttgataatgc gcgacataat ccgcgcactg gtgatatcgc gacctttttt aatctgctgt
     1501 ttttcaaact gattaatcag cagaaatagc ttaatcgtca gaataaccgc aatcagggac
     1561 gtacagataa aatcgataac gcctggggtg ataaatttga gtttatagtt ttctataaca
     1621 taattaatga tgctaccaac agcactgata attatggtgt agattaaaaa ttgcaccgca
     1681 tggaataaaa atccttttct tttacgattt ccacggcgaa accaaaagct catcagaatc
     1741 aatgctgcgc agctaccgaa aataatgacc agattaagcg cattatttgt aaacagttca
     1801 gcgatcattg ttttatcagg ctcctccaga taattgtcgt catgccggaa acccctggcg
//
LOCUS       DAJTYI010000249         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYI010000249 DAJTYI010000000
VERSION     DAJTYI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..152)
                     /locus_tag="OO564_004014"
     CDS             complement(<1..152)
                     /locus_tag="OO564_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654897.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAE"
     gene            1054..1338
                     /locus_tag="OO564_004015"
     CDS             1054..1338
                     /locus_tag="OO564_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6654898.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1326..>1810
                     /locus_tag="OO564_004016"
     CDS             1326..>1810
                     /locus_tag="OO564_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6654899.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          456 a          386 c          522 g          446 t
ORIGIN      
        1 atttcggcgt ttccggcgta ggtgtgccac gaatgcatcc agtggcgtga tgttggatgc
       61 aatgctgtat gagagatgag gggcggcgtc cagacgcagc cttcaaaccg ccttgagctg
      121 ctgccgttaa gagcggtggt ggtgagcgtc aaggttaaaa ggcgcagaac atgtgtcagg
      181 taggtgttgc ggagatgaac gaaagtgaac tgtcgatgaa gtgtcgaaag gaacctctag
      241 gcgatgtcga aaatgtgcta tctacgcagc gcatgacgag tccagcagat atctggtact
      301 ggctgggcgg catccggcat ataggcagca tgaacgcaac acaggctctc gtacggaacg
      361 tgggaacctg tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct
      421 gagtaccgat gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat
      481 gggcatggag cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg
      541 gaggagccca tgagtcagcc aaagccgttc gaggtctcta agtatgcggt ttggaaagca
      601 taccagcggg tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg
      661 tttgaggtta ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca
      721 agtggattgc ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt
      781 tactgcgaat tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata
      841 cttgaatggt tcaacgacaa gagccgtatg acgcgagagt gtcacgtacg gttccgtggg
      901 agcgtagggg ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg gttacgtcca
      961 cagaggtaaa tgccgctgac gcggcatctg taatctgtta ctttgtgtat atcatttgct
     1021 atatattgtc aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca
     1081 ggtgttcagc tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca
     1141 gaaattcttc ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa
     1201 gatgttattc tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt
     1261 gaaatgcttg atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa
     1321 cctcaatggg atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat
     1381 ttgtcagcgg tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg
     1441 caatcggagc cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt
     1501 tttactcact ggccactggt agtatcaatc atacagaagc tacaggtaac cttcggcgca
     1561 atatgccaga ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc
     1621 gaggaaaagg gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg
     1681 ccgaaaatat cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt
     1741 tttatattca tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac
     1801 tccctcaata
//
LOCUS       DAJTYI010000250         1797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYI010000250 DAJTYI010000000
VERSION     DAJTYI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..210
                     /locus_tag="OO564_004017"
     CDS             <1..210
                     /locus_tag="OO564_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654900.1"
                     /translation="VRQEIDAFFCAFREKVEQLRNTLIQSSEDHKRDQQAQERLTRRL
                     QALNERVPELITDSKALREELETML"
     gene            207..971
                     /locus_tag="OO564_004018"
     CDS             207..971
                     /locus_tag="OO564_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6654901.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGD"
     gene            993..1112
                     /locus_tag="OO564_004019"
     CDS             993..1112
                     /locus_tag="OO564_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654902.1"
                     /translation="MIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            1109..>1797
                     /locus_tag="OO564_004020"
     CDS             1109..>1797
                     /locus_tag="OO564_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6654903.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWR"
BASE COUNT          421 a          438 c          529 g          409 t
ORIGIN      
        1 gtccgccagg aaatcgatgc ctttttctgc gcattcaggg agaaagttga acaactgcgt
       61 aacacgctga ttcagagctc tgaagatcat aaacgcgatc agcaggcaca ggaacggctt
      121 accaggcgac ttcaggcatt aaatgaaagg gttcctgagc tcattactga cagtaaggcg
      181 ctgagggaag agctggagac aatgctgtga cttcaccatt tattcaacaa attgctgata
      241 accgggtatg tcaggtactc acctgtcttc ctgaaaaatt tgtggttgat ttcgcaaacg
      301 gaatcgatgt tgcacaggag catatccgca cggccggaga gcgcacgttc ttccggcgtt
      361 taaaagaggg actgaccggc gaaggcgctg cacggcagaa tgccatcaat gcttcggttg
      421 cacagggcgt tgaggcgtcc ctgcgctggc tgacggagct gacaacgtca cttgccacca
      481 caaactacgc gattacccgg gtaaacgaca gggtcagttc actggtcagt gatactgcca
      541 ggttggcgca ttattccgca gatacacggg agcagttact catcctggcc gatcaggttc
      601 accataaact gaatcatctt gaagaaaaac tccaccgtgt tgaccaggtt cagcgggcgc
      661 agttacatct tgagcagata ttctcatggt ggagcgccgg gcgatacgcg tcattttccc
      721 ctgccggacg ttgttatgtg gcacttgaag agcttcgctg gggcgcgttt ggtgatgtga
      781 tacgtcaggg cgaaacaggc caggttaacc agctactgga tattctcaga cataaagcat
      841 taacgcagat ggcacaggag agtggcggta gtgcaaccgt gcgtctgaac actctggact
      901 ggcttggtgg tcagggccgg gagcaggcag ataacgagtg gcatgacgcg attaactggc
      961 tgggagacta gtgcagtgaa gagcagcatc cggtgatctg gtcaaccacg caggctgcag
     1021 aacacttacc ggttcgcatg cctcgcctct gctctgcaga acgcctctct gaaagtatgg
     1081 tcgatgaaat atttcagaag ggggctgcat gagtacagaa atgaaaacgg ggctggtgct
     1141 gtccggtggc ggggcggtgg gcgcttatca ggcgggagtg gttaaggcac tggcagagtg
     1201 tgatacacag atcagcatgg tttcaggggc cagcattggc gcattcaatg gtgccattat
     1261 cgcgacctct cccgatctgt cagaagctgc cgtacgcctg gaagcgctct gggatcatct
     1321 ggggaataat caggtgctgt cggtaaaccg attggtttac ttttcattgc tgaaaaaatt
     1381 gttccaggca atgaacctct gccagatccc cggacgtgca ggagcactgc ttacgacgct
     1441 tcttcgccat atatcgatac tcaacgggtt tgacaatctg atggctcagc cgttgttgtc
     1501 agatgagccc ctgacagcgc tgttggatca ttatcttgat actgatgctc tggcagacgg
     1561 gctaccgctg tatgtgtcgc tgtaccccac agaagggggc atgcaggata ttattgactg
     1621 cattcgtgct gaactgggtg ccggaaccac gaaaaacgcc gtttttcagc atatccagag
     1681 cctgccccgc ggacagcaga aagaggctct gcttgcgtca gccgcgctgc ccctgctgtt
     1741 ccgtccccgt gaggttcagg ggacaatgtt cggtgatggt ggtatgggag gatggcg
//
LOCUS       DAJTYI010000251         1655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYI010000251 DAJTYI010000000
VERSION     DAJTYI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(132..849)
                     /locus_tag="OO564_004021"
                     /pseudo
     CDS             complement(132..849)
                     /locus_tag="OO564_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
     gene            complement(846..1211)
                     /locus_tag="OO564_004022"
     CDS             complement(846..1211)
                     /locus_tag="OO564_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6654904.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            complement(1212..>1655)
                     /locus_tag="OO564_004023"
     CDS             complement(1212..>1655)
                     /locus_tag="OO564_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6654905.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
BASE COUNT          381 a          453 c          349 g          472 t
ORIGIN      
        1 tgaccaccag cacgtttaac gtcctgtgcc atttttcagg cataaaaaaa ccgctcggcg
       61 gcgggttttg cttactttac catcgcgtac aaaatcggca aagtatcaga tttgcatgaa
      121 atatatgcct tttaatctac ttttgcaata ctttgctttg aaaatgccgc tttttgttct
      181 gaacgtgttc tcatcacaaa caataaagcc tcactatcca gtcggtgaaa aatgtgtttc
      241 attgcaatcc agtgacgagt aaatgttttg gaccagtttt tagttgtcac tcccgccagt
      301 aaatgccagc tcctggtatt cataaccttc cccaccaacc tggtattcat aaccttcccc
      361 accaaaaagt tctgctttta ctgcctgcgc cgccagccag attaattttt tcaggcgttc
      421 ctgcgttttc cctgcaattt ttctggtacc ggattgagca ttaaattcat tccacgccca
      481 ctgtgttatc gcgatctgat attcccaaca aatactcccg ctgtaacacc acaacaacca
      541 ggctttatga tgttcttcaa gagacagaac agcccgccgc cacgatgatg tcgaaaactc
      601 aaccggactg accagaggaa ttgacgtccc cttcgccagc gattgctttc ccgggattgg
      661 tggattatcc cgcgttatca tttttccagt cacttcatcg cggtaccgga tttttttacg
      721 cctgtaacgc cctgtatcga acatggcatt ctcttgccag gcttcaagct gaccttttgt
      781 tgccccactc aaatcagcgg tggcgataat gagctgctca cgcacaaact gtaaatactg
      841 gttattcatg cgcaccccag ttctgtgatt tttatcccca gccgcccacc aggaacgagc
      901 tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt ctataagtag tccagcatgc
      961 gtcagcgcat ccagcggtgc cttcaggata ttgtccaggt cacgacggcg cttatccggt
     1021 ggctctgcaa taatctttat cgccagcctt ccggacaggt ttaatttcag tcgctgctgg
     1081 cgaacaatta gcgccacatc acggcgataa cgctcaccgg cttttgatac aaaatatgtg
     1141 ctgccacgac gtcgccagta ggtgttcacc gttggcgggt aaggcaaaac aaattctatg
     1201 cgttcggtca tttatgcttt ccacttcaga acacccgaat ttctcgcgtg cattaaaaaa
     1261 cgaatcagca acaacaactg actaccgtgt ttttcttcaa aatcttttac cccggcgtgt
     1321 agttcgttat ggcatttacg acacagcgga ataacaaaca aatcgtcagc ctttgttccc
     1381 atccctccca gtccatgacc aatgatgtgg tgcggatcat ctgcctgatt gccacacgtc
     1441 atgcatttct gcgtttttac ccagcgcgtg tatacaggca tctcttcccg ttgtggtttc
     1501 tggcgctgga gatactgagc cggagattcc ggatcaacgg caatgctgac caccgtctct
     1561 tcctgtggtg gattctgttg ctggtgggcg tgaggcagcg gcgcaagatt ttttgtgcgc
     1621 tgcttcagta tgctggtggc ggtctgctct cccgg
//
LOCUS       DAJTYI010000252         1611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYI010000252 DAJTYI010000000
VERSION     DAJTYI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(84..767)
                     /gene="cusR"
                     /locus_tag="OO564_004024"
     CDS             complement(84..767)
                     /gene="cusR"
                     /locus_tag="OO564_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6654906.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            924..>1611
                     /gene="cusC"
                     /locus_tag="OO564_004025"
     CDS             924..>1611
                     /gene="cusC"
                     /locus_tag="OO564_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6654907.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIA"
BASE COUNT          394 a          431 c          427 g          359 t
ORIGIN      
        1 caaaggcgaa aaacgccgcg atggtggcca ggctgataaa aaaggtcagg cgggttgcca
       61 gcgaaaacgg gcgctgaaat ggcttactga ccatccggca cctcaagcat gtaacccacg
      121 ccgcgcacgg tttgaatcag cttcggctca aagtcgttgt cgattttgcc gcgtagccgc
      181 ttcaccgcca catcaatggc gttagtgtcg ctgtcgaaat tcatgtccca tacctgcgag
      241 gcgataagcg agcggggcag cacttcgccc tgatggcgga gaaaaaactc cagcagagta
      301 aattctttac tggtcagagt gatgcgcgtg ccgctgcggg tgactttgcg gctgacgaga
      361 tcgaccatca gatcggcaac ctgaaactga ctttcgataa tcaccgccgc cccgcgccgc
      421 aggagagtgc gcacccgcgc cagcagttca gcaaaagcga acggtttcac caggtagtca
      481 tctgccccca actccagccc cttgacgcga tgttcaatgg tgccaagcgc ggtaagcaac
      541 agaatcggca tccctttatt ggcggagcgc aacatgcgca cgatatccca gccgttcacg
      601 tctggcagca taatatcgag gattatcagg tcataatcac cggtcatcgc cagatggtag
      661 ccattcagcc cgttgtcggc caaatcgacc acaaaaccgg cttcggttaa ccctttggtc
      721 aagtattctc cggttttctt ttcatcttcg acaatcaaca gtttcatatt tcctccgcat
      781 gttgcccggg caattctaga gtagcgggat cagatggcaa tcgcttattg gcaaaatgac
      841 aattttgtca ttttcctgtc accggaaaat cagagcctgg cgagtaaagt tggcgacata
      901 aaatcaccag aaattatgag cctatgtctc cttgtaaact tctgccattt tgtgtggccc
      961 ttgcgctaac tggttgttca ctggcaccgg attatcagcg tccggtaatg cccgtaccgc
     1021 agcagttctc actcagccag aacggcctgg ttaacgcagc ggataactac cagaacgcgg
     1081 gctggcgcac cttttttgtt gataatcagg tgaagacgct gattagcgag gcgctggtga
     1141 ataaccggga tttgcgcatg gcgacgctga aagtgcagga ggcacgggcg caatatcgtc
     1201 tgaccgatgc cgaccgctac ccacagctca atggcgaggg cagcggcagc tggagcggca
     1261 atcttaaagg cgattcagcc acgacgcggg agttctcgac tggccttaac gccagctttg
     1321 atctcgattt tttcggtcgc ttaaagaaca tgagcgaagc cgagcgccaa aattatttag
     1381 ccactgagga agcccagcgc gcggtgcata ttttgctggt ttctaatgtc gcgcaaagct
     1441 atttcaatca gcagctggcg tatgcgcaat tgcaaatagc cgaagaaacg ctgcgtaatt
     1501 atcagcagtc atatgcgttt gtcgaaaaac aactgttgac cggtagcagc aatgttctgg
     1561 cgctggaaca ggctcgcggg gtgatagaaa gtacccgcag tgacatcgct a
//
LOCUS       DAJTYI010000253         1553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYI010000253 DAJTYI010000000
VERSION     DAJTYI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..155)
                     /locus_tag="OO564_004026"
     CDS             complement(<1..155)
                     /locus_tag="OO564_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6654908.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQR"
     gene            complement(202..921)
                     /gene="yehT"
                     /locus_tag="OO564_004027"
     CDS             complement(202..921)
                     /gene="yehT"
                     /locus_tag="OO564_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6654909.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(918..>1553)
                     /gene="btsS"
                     /locus_tag="OO564_004028"
     CDS             complement(918..>1553)
                     /gene="btsS"
                     /locus_tag="OO564_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6654910.1"
                     /translation="LSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAV
                     IRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNI
                     AIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGL
                     YQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          378 a          416 c          354 g          405 t
ORIGIN      
        1 caacgctgaa aaccctcttc gtcttcttta cgtagccaga cggcgatctg ttccgcggtg
       61 gcttcgacat tatccagcgc cagaatacgc accaggtcat tattattggc tttcaaaatg
      121 tagcgcacgc tgcgcagaat atcgttactt atcatgaggc cttcaggtgt tgatgaggca
      181 aaaagccatt ttagcagtct tttacaggcc aatcgcctct tttaagcttt tcagataacg
      241 gcggctgacc ggcacggtta agccattacg caaaatcaac tcggcctggc cgttatcttc
      301 cagacgaatc tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc agcgcagtag
      361 tggtgtacga ctttccaggg tacgtaatgt caattcggta aagccctctt tcccttcgtg
      421 gctggtaacg tagacaccgc tcatccgact gctgacaaat gccacatctt tcatttgcag
      481 caaataaatc cgactatgcc ccgtacaagg gataaatttc agcgcctgtt gattttccgg
      541 taacagcgaa acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg ttttctccag
      601 tcgcgcttca tcaattggct tcagcagata atcaaaggca tgttcttcaa aggctttaat
      661 agcgtattcg tcaaacgcag tgagaaaaac aatatacggg cgatgttccg ggtcaagcat
      721 ccccaccatt tccagaccac tgatgcgcgg catctggata tcgagaaaca gcacatccgg
      781 gcgcagttta tgcaccgcgc cgatcccttc cacggcgttt gaacactctc caacgatttc
      841 aatatcgctc tgctcctgca aaaatatacg caggttctcc cgtgctaacg gttcatcatc
      901 gacaattaag actttaatca tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg
      961 taactatcag gctcacaggc gacgcttatt ccatagtcat cgccaaaccg ttcacgtaaa
     1021 cgcttatcca ccagattcat ccccagccca ctggcattgg ttaccggttg atacaaaccg
     1081 gcattgtctt cgatctccag catcaaatgt tgcccctcac gtcgggcgct gattgccact
     1141 cgccctgtat ccagcagttg tgatgtccca tgtttaatgg cgttttccac tatcggttgc
     1201 agggtaaacg cgggcaattg ctgctgggat aattcttgcg gaatagcaat gttgacctgc
     1261 aaccgcgact ggaagcgcgc cttttcaatt tgcagataag cgttcacatg ttcaatttcg
     1321 tcggcgagag taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga
     1381 tactgcacca gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta
     1441 agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat ttctgactgg
     1501 gtgagcatcg ctttttgccg ctcatattgc ccggcaagaa tctgcgccga aag
//
LOCUS       DAJTYI010000254         1530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYI010000254 DAJTYI010000000
VERSION     DAJTYI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            100..405
                     /locus_tag="OO564_004029"
     CDS             100..405
                     /locus_tag="OO564_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654911.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            632..>1530
                     /locus_tag="OO564_004030"
     CDS             632..>1530
                     /locus_tag="OO564_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6654912.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAW"
BASE COUNT          511 a          334 c          376 g          309 t
ORIGIN      
        1 aaccggagaa tggcagtgga catttcaggt cggggacctt gaaaacgtca atatcactca
       61 ctgggcagta atgccaaaag caccggaggc tggagcataa tgaccacttt taccgacaaa
      121 gaactgatta aagaaattaa agagcgtatc agcagccttg acgtgcgaga cgatattgag
      181 cgccgtgctt atgaaatcgc actcctatct ctggaagtag aaccagatga acgcgaagct
      241 tatgaattat tcatggaaaa gcgtttcggt gacttagtag atcgtcggag agcaaaaaac
      301 ggcaataacg aatacatggc atgggatatg actctcggtt ggatcgtctg gcagcaacga
      361 gctggtatcc atttttcaac aatgtcacag caagaggtga aataatggag ccatacagcc
      421 tcacactcga tgaggcctgt cattttctca agatatccag accgactgcc attaactgga
      481 tacgcacagg gcgtcttcag gcaacacgca aagatcccac taagaataaa tctccttacc
      541 tcacaacacg acaagcctgc attgcggctc ttcagtctcc gctgcatact gtccaggtga
      601 gcgcgggtga tggcataaca gaggaaagaa aatgtcactc ttccgcagag gtgaaatatg
      661 gtacgccagt ttcacattgc cgaacggtaa aagatttaaa cagtctcttg gaacaaagga
      721 caaaaggcag gcgacagaac tccatgacaa gctaaaggct gaagcatggc gggtcagcaa
      781 acttggtgaa atacctgata taacgttcga ggaagcgtgt gtcaggtggc ttgaagagaa
      841 agcacataaa aaatcactgg acgatgacaa aagccggatc ggattctggc ttcaacattt
      901 cgcaggaatg caactaagag acattactga atcaaaaatt tattcagcaa tgcagaaaat
      961 gacgaaccgg cgtcatgagg aaaactggaa actcagggca gaagcatgca gaaaaaaagg
     1021 gaaacctgtt ccagaataca cgccaaaacc agcgtccgtt gtaacgaagg ctacgcatct
     1081 ttcatttata aaggccctac taagagccgc agagcgtgaa tggaaaatgc tggataaggc
     1141 accaattatt aaagtgcctc aaccaaagaa taaacggatc cgctggctgg agccccatga
     1201 agcacaaagg ctgattgatg aatgtccgga gccattaaag tctgttgttg aatttgcact
     1261 ggcaacaggc ttaagacgct cgaacatcat caaccttgaa tggcaacaaa tagatatgca
     1321 gcgccgggtg gcatggataa acccggaaga gagtaaatca aaccgcgcaa tcggcgttgc
     1381 gctgaatgat actgcatgtc gcgttttgaa aaaacaaatc ggaaatcatc accgttgggt
     1441 atttgtgtac aaggaaagct gtaccaaacc agacggaacg aaagcgccaa cagtaaggaa
     1501 gatgcggtat gacgcaaaca cagcctggaa
//
LOCUS       DAJTYI010000255         1493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYI010000255 DAJTYI010000000
VERSION     DAJTYI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..1071)
                     /locus_tag="OO564_004031"
     CDS             complement(<1..1071)
                     /locus_tag="OO564_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6654913.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          447 a          238 c          321 g          487 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc cca
//
LOCUS       DAJTYI010000256         1378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYI010000256 DAJTYI010000000
VERSION     DAJTYI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..368
                     /gene="etp"
                     /locus_tag="OO564_004032"
                     /gene_synonym="yccY"
     CDS             <1..368
                     /gene="etp"
                     /locus_tag="OO564_004032"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6654914.1"
                     /translation="RKRLPGVKVKSAGVHGLVKHPADATAADVAANHGVSLEGHAGRK
                     LTAEMARNYDLILAMESEHIAQVTAIAPEVRGKTMLFGQWLEQKEIPDPYRKSQDAFE
                     HVYGMLERASQEWAKRLSR"
     gene            388..>1378
                     /locus_tag="OO564_004033"
     CDS             388..>1378
                     /locus_tag="OO564_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCR6654915.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEI"
BASE COUNT          381 a          328 c          363 g          306 t
ORIGIN      
        1 tgcgtaaacg tctgccgggc gtaaaagtca aatcggcagg tgttcatggt ctggtaaaac
       61 accctgcaga tgcgacagcg gcagatgttg ccgctaatca tggcgtatct cttgaaggac
      121 atgctggacg taagctcact gctgagatgg cacgaaatta cgatctgatt ctggcaatgg
      181 agtcggaaca tattgctcag gttaccgcaa ttgctcccga ggtccgcggg aagacaatgc
      241 tgtttgggca atggctggaa cagaaagaga tcccggatcc ctatcgtaaa agtcaggacg
      301 catttgaaca tgtctacggt atgttggagc gcgccagtca ggaatgggcg aagcgcctca
      361 gccggtaatt tgagtttata aaatacgatg acaactaaaa atatgaatac gccactaggc
      421 agcactcagg aaaatgagat cgatctgctt cgtctggtcg gcgagttatg ggatcaccgt
      481 aagtttatta tcagcgtgac cgcgttattc acgctgatcg ctgtcgctta ctcgctgtta
      541 agcacaccaa tttatcaggc agatactctg gtccaggttg agcaaaaaca gggcaacgcc
      601 attctcagcg gcctgagcga tatgctccct aactcatcgc ccgagtctgc accggagatc
      661 caactgctgc aatcgcgcat gattctcggt aaaaccattg ctgaactgaa tctgcgcgac
      721 atagttgagc agaagtattt tccgattgtg ggtcgcggct gggcgagatt aaccaaagaa
      781 aaaccaggtg agctggcgat cagctggatg catattccac aactgaatgg tcaggatcag
      841 caactgacac tcacggttgg ggaaaacggc cactatacac tggaaggtga agagttcacc
      901 gtcaatggta tggtcggcca gcgtctggaa aaagatggcg ttgcgctgac tatcgcggac
      961 attaaggcca aaccaggaac acagtttgtc ctgagccagc gtaccgaact ggaagcgatt
     1021 aacacattgc aggaaacctt taccgttagc gaacgcagta aagaaagcgg gatgctggaa
     1081 cttaccatga ctggtgatga tccccagttg attactcgta ttctgaacag catcgctaac
     1141 aactatttgc aacagaatat cgctcgccag gcggcgcagg attcacaaag ccttgaattc
     1201 ttacagcacc agttacctga agtgcgcagc gagctggacc aggcggaaga aaaactcaac
     1261 gtttatcgcc agcagcgcga ttcggttgac cttaacctgg aagccaaagc cgttcttgag
     1321 cagattgtga acgttgataa tcaactcaat gagctgacct tccgcgaggc agagatct
//
LOCUS       DAJTYI010000257         1375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYI010000257 DAJTYI010000000
VERSION     DAJTYI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            50..385
                     /locus_tag="OO564_004034"
     CDS             50..385
                     /locus_tag="OO564_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6654916.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            478..561
                     /locus_tag="OO564_004035"
     CDS             478..561
                     /locus_tag="OO564_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654917.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            661..>1375
                     /locus_tag="OO564_004036"
     CDS             661..>1375
                     /locus_tag="OO564_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6654918.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLI"
BASE COUNT          354 a          332 c          351 g          338 t
ORIGIN      
        1 tgccattccc agggatatag ttatttcaac ggccccgcag tggggttaaa tgaaaaaaca
       61 aattgagggt atgacaatga aaaaagtatt agctctggtt gttgccgctg ctatgggtct
      121 gtcttctgcc gcctttgctg cagagactgc gaccacacct gctccgactg cgacgaccac
      181 caaagcagcg ccagcgaaaa ctacacatca taaaaaacag cataaagcag cacctgccca
      241 gaaagcgcag gcggctaaaa agcatcataa aaatacgaaa gctgaacaga aagcccctga
      301 acaaaaagcg caggcagcga agaaacacgc cgggaaacac ggccatcagc aaccggcaaa
      361 acctgctgca caacccgcag cgtaagtttt caacagtaat gctggcgcgc cccctcgcgc
      421 ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga gggtagtatg
      481 gtgggccgtt atcgctttga gttcattctt atcatcctta ttttatgcgc actgattgcc
      541 gcccgttttt atctttcctg actgtagtta tctgatttta ctcccacttt catcccgtcc
      601 cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc agagtgtgat
      661 atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc ttttgtgttt
      721 gcagataaac cagacgttgc caaatcggca aacgatgagg tcagcaccct gttttttggt
      781 catgatgatc gtgtgccagt gaatgacacg acccaatcac cgtgggatgc ggttgggcaa
      841 ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa tctggcatta
      901 acggcaggac actgtttatt gacgccgcct aagggtaaag cggataaggc ggtagcgctg
      961 cgttttgtgt caaataaagg tctttggcgc tatgagatcc acgacataga aggccgcgtt
     1021 gatccgacac tgggaaagcg gttaaaagca gatggggatg gttggattgt tcctcccgca
     1081 gctgcaccgt gggacttcgg attgattgtg ctacgtaatc ccccttctgg cattacaccg
     1141 ttgccgttat ttgagggaga taaagccgcg cttactgccg cattaaaatc ggctggtcgt
     1201 aaagtgactc aggcgggata ccctgaagat catctcgata cgttatacag tcatcaaaac
     1261 tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcac atcagtgcga taccttgccg
     1321 ggtgacagcg gttcgcctct gatgttgcat accgatgacg gctggcaatt aattg
//
LOCUS       DAJTYI010000258         1370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYI010000258 DAJTYI010000000
VERSION     DAJTYI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..276)
                     /locus_tag="OO564_004037"
     CDS             complement(<1..276)
                     /locus_tag="OO564_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6654919.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLS"
     gene            complement(877..1125)
                     /locus_tag="OO564_004038"
     CDS             complement(877..1125)
                     /locus_tag="OO564_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6654920.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          340 a          237 c          317 g          476 t
ORIGIN      
        1 gcttaaatta tttttactta tattaattga tttaattccc ggggggattt caggtaatgt
       61 tgtcaggcct aattcagaca agtccaggtt cgtctcttgg ttttgtagac atgatactag
      121 tcgctgaaaa gcgatgtctc gttgttcttc ttgtatgcgg ttgtttttcc attcagtcca
      181 atgggttaga taattttcat atgcgcggct agtgtcgatt gaataagtgg agaacgaatt
      241 tgaaattaat ctgtgattgt tatttgtcgg gagcataaat atcagggcgt attctttatg
      301 ttgagaggtg ttggattctt ttttatctgc tttgcaatgt tattgattcc cttcagcagt
      361 agacaggaac ttctggagga ggcggatata tagtttgtgt aagcatatta actccatatg
      421 ttatatattg aagaacttct gctttacact atcctaaact ggatcggttt aatgtaaaaa
      481 aacgatactt tttgggggag ggggcaattt ttccttaagg tatgaaaaaa ggagctgaag
      541 ctatataata gctattaatg ccactggtat cagtgaatca tgtatataaa aaactgtgtt
      601 aaggaggctg tccattagcg gttttttatt cttctggaac cgcggcatct ttgcgtatct
      661 ttttgtcttc tcaccgtctg gtcggtgttt tgctgagact gctaatttcc tgtctttgct
      721 agtgctgtac tggtactgcc caatcatgat tggcgggggc gggcggagtt gagaccgcag
      781 acacgtcgta tgcaagaacg tgctgcggct ggctggtgaa cttccgatag tgcgagtatt
      841 gaatgatttc cagccgttac tgattttacg tgctaattag tgaacaaacc actcgtcagc
      901 agactcccag gtatctttca gagtttcctg aacaaaagtt ttagctgaat ctttatcggc
      961 ggtgcgcgta acagaaaggc catcgttgct ggtggctttt acgatcacct ctacatcgtc
     1021 ataacgttta ctgatgcgtc gggttaattc ttcctttaac gcatccacag caccggttgg
     1081 cattttagtc attttctctt tggctatgca gatttcaata cgcataaaaa tccctctaca
     1141 ctgtgttttt atacagtatt atttttaact gtatggataa acagtgtcaa gagtgaggga
     1201 aagtgatttt ttggggcatg catggggcaa aaatatttgt tgtggggcat tttggggcat
     1261 gattgggaca ctttgttaca tatgaacttt gccgattttc atatgacatg gcaatacaac
     1321 gctttgatta ttctaaagaa aacacatgct cttgggcgtt ctttagttat
//
LOCUS       DAJTYI010000259         1339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYI010000259 DAJTYI010000000
VERSION     DAJTYI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..606
                     /locus_tag="OO564_004039"
     CDS             <1..606
                     /locus_tag="OO564_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6654921.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            638..1246
                     /locus_tag="OO564_004040"
     CDS             638..1246
                     /locus_tag="OO564_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654922.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          395 a          266 c          341 g          337 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgaccca
//
LOCUS       DAJTYI010000260         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYI010000260 DAJTYI010000000
VERSION     DAJTYI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>1288
                     /locus_tag="OO564_004041"
     CDS             <1..>1288
                     /locus_tag="OO564_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HCR6654923.1"
                     /translation="QATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVW
                     CTSLPGNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIR
                     GALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFS
                     FYRLIDKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVN
                     GREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIAS
                     KLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQ
                     RLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCV
                     LDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYY"
BASE COUNT          336 a          288 c          297 g          367 t
ORIGIN      
        1 acaggcgact caaacagcgg ttaacattgc cgggaaggaa tgcgacctcg aggagcaata
       61 tcagcttggc actgaagcag ctctgaaacc tcacctgcgc acaatcatca ttctcaaaca
      121 gggaatagtc tggtgtacat ccctgcctgg gaatcgggtc ctgctgtctc gtattcctgt
      181 tgtcccggac agtaatttac tgttggctcc ggcaatcgac accgttaata gattacctat
      241 cctgctctat cagagccaat ttgcagatac gcgcattttg gttacgataa gtgatcaaca
      301 tattcgcggg gcgcttaatg tacccttgaa aggggtaagg tatgtattac gcgtggcgga
      361 tgacattatt ggaccaacgg gtgatgtgat gacgcttaat ggacattatc cctataccga
      421 gaaggttcac tccacaaaat atcatttcac tattatcttt aacccgccgc cactctttag
      481 cttctacaga cttatcgata aaggctttgg gattttaata tttattctgt taatcgcctg
      541 cgccgctgcc ttcctgcttg atagatattt caataaaagc gcaacgcctg aagagatcct
      601 gcgacgggca ataaataatg gggagatcgt ccctttttac caacctgtgg taaatggtcg
      661 ggaagggaca ttgcggggag ttgaggtgtt agcccgctgg aaacaacctc acggtggata
      721 tatttcaccc gcggcattta ttccacttgc tgaaaaatcc ggattaatcg ttccgcttac
      781 acaaagcctg atgaatcagg ttgccagaca gatgaacgct atcgcgagta aactgccgga
      841 aggttttcat attggaatta attttagcgc ttcgcatatt atttcgccga cgtttgtcga
      901 cgagtgttta aatttccgtg acagttttac ccgccgcgat ttaaaccttg ttctggaagt
      961 caccgagcgt gagccattga atgttgatga aagtctggtt cagcggttga acattctgca
     1021 tgaaaatggt tttgtcatcg cgctggatga tttcggtact ggctactcag ggctttctta
     1081 tcttcatgac ctgcatattg attatatcaa aattgatcac agtttcgttg gccgcgtaaa
     1141 cgcagaccca gaatcaaccc gaattctgga ttgtgtattg gatctggcgc gtaaactttc
     1201 gatcagtatc gtcgctgaag gtgtcgaaac gaaagaacaa cttggctatc tgaaccaaaa
     1261 taatatcaca tttcagcagg gttattat
//
LOCUS       DAJTYI010000261         1274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYI010000261 DAJTYI010000000
VERSION     DAJTYI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            326..1075
                     /locus_tag="OO564_004042"
     CDS             326..1075
                     /locus_tag="OO564_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6654924.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          364 a          256 c          256 g          398 t
ORIGIN      
        1 actcaggcta cctcaaaaag ttatcggtgc gtttatcgaa aaacatatgt tctactaatt
       61 ggagtcattg tctgtaaaag caactcaaaa atacaagttg cttattcccc ttgttggtaa
      121 gaacttaatt ctaactgttt gttctggatg ttttaaacgt cctccttttt atgcttgttc
      181 ataacggata tttaataggt aacctttgtt ttagcatctt ctgatttatt taaattatgt
      241 gatagcgcac acaattttat tatccagtac tttatactct taataaaata attataaata
      301 tttatcctct ataaggagat gttatatgaa aagacagctt tttactctta gtattgtcgg
      361 ggtattttct ttaaatacct ttgcatcatt ccctccggga aatgatgtga caacaaaacc
      421 tgacctttac tacctgacaa acgataatgc tattgacagt ctggcattat taccgcctcc
      481 accacaaatc ggaagtattg ccttcctgaa tgaccaggcc atgtatgaaa aggggcgttt
      541 attgcgaaat actgaaagag ggaagctggc agctgaggat gccaatctca gtagtggtgg
      601 cgtggcaaat gtattttctg ctgcttttgg ttctccgata acggctaaag attcaccaga
      661 attgcataaa ttactgacaa atatgattga ggatgccgga gacctggcaa cgcgttcagc
      721 aaaagaatat tatatgcgta ttcgaccttt tgctttttat ggtgtttcta cgtgtaatac
      781 aaaagaacag gatacattat ccagaaatgg ctcttatcca tcaggtcata catcgattgg
      841 ttgggcaaca gcacttgtat tgtccgaaat aaatccggca cgccaggata ccattctgaa
      901 acggggttat gaactggggg acagcagggt tatttgcggt tatcactggc aaagtgatgt
      961 tgacgcagca cgtattgttg gctctgctat tgtagcaaca ctgcactcaa atcctgtgtt
     1021 ccaggcacaa ttacaaaaag cgaaagatga attcgctaac aatcagaaaa aataatgctt
     1081 ttagccagta cgccttataa ccccgacctt cagggctaat gccagtcagt taagcaactg
     1141 actggctctt tttcagggct gtggggtatt tccagggcct ctcctttacc actctcggga
     1201 aggccctttc ccttcttgtc ggtaatttca ctaggctgtg tcccttaatt acgtaagcgt
     1261 tgataaaaga gtcg
//
LOCUS       DAJTYI010000262         1274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYI010000262 DAJTYI010000000
VERSION     DAJTYI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..592
                     /locus_tag="OO564_004043"
     CDS             <1..592
                     /locus_tag="OO564_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654925.1"
                     /translation="TIRAIAQRLGLSHSTIHTLFQRFLASGIAWPLPDSVSFAQLDAI
                     LYANRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVARIAREHDINNN
                     LLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGN
                     VPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            589..936
                     /gene="tnpB"
                     /locus_tag="OO564_004044"
     CDS             589..936
                     /gene="tnpB"
                     /locus_tag="OO564_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6654926.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            956..>1274
                     /locus_tag="OO564_004045"
     CDS             956..>1274
                     /locus_tag="OO564_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6654927.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          340 a          329 c          342 g          263 t
ORIGIN      
        1 gaccatccgg gccatcgctc agcgtctggg tctcagtcat tccacaatac atacgctttt
       61 tcagcgattt cttgcatccg gtatcgcatg gccattgccc gattcagttt cattcgctca
      121 acttgacgcc atcctttatg ccaacagaaa gaaggaatta acagagcctc aaatcagaga
      181 aggctcatgg cgaaaagaac ggcgaaccag ctatagccgt gaatttaagg tccgtctggc
      241 taagcaggcg ttacagcctg gggctgttgt tgcccggatc gccagagaac acgatatcaa
      301 taataacctg ctgtttaaat ggaaaagcca gtacgaggac ggcttactga gcgatgatga
      361 catacaggaa tgcatgcctg tcccggtggc actgactgat acgccggagc cgaccagacc
      421 agttacaaat cccttctggc gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa
      481 cgtcccacgg tgcgagctgc atcttaaatc aggtgtggta aaactgtttg accctctcac
      541 tccggaactg ttacgggcgc taatccgcga aatgaaaggg ggtatccgat gataacgctg
      601 ccgaccggta ccaaaatctg gatcatcgct ggcatcacag atatgcgttg tggcttcaat
      661 ggcctggctt cgaaggtgca gaacacgctg aaagatgacc cgttctccgg gcatatcttc
      721 gtcttccggg gccgcagtgg caaaatggtg aaaatactgt gggccgatcg tgacgggtta
      781 tgcctgttcg ccaaacgcct ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg
      841 aaagtgcacc tgacgccagc tcagttatcc atgctactgg aggggatcgc gtggcaacat
      901 cccaaacgga cagaacggcc tggcatccgg atataacccg tgataaaaca agggaatgaa
      961 caatgaactc cccgatgata ttgagctgct taaagccatg ttgcgtaagc aacagagtcg
     1021 gcttcgacag tatgcctgtc aggtcgcggg ctatgagcag gaaattgaac ggctgaaagc
     1081 gcaactcgac aggttgcgtc gtatgttgtt cggccagagt tcagagaaaa agcgtcataa
     1141 gcttgaaaat cagatccgac aggcagaaaa acgactgtcg gaactggaaa accggctgaa
     1201 cacagccaga aatcttctgg aagatgcatc gtcagtcaca gattcacctg acaccagtcc
     1261 cccgtcagaa aacc
//
LOCUS       DAJTYI010000263         1269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYI010000263 DAJTYI010000000
VERSION     DAJTYI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..588
                     /gene="azoR"
                     /locus_tag="OO564_004046"
     CDS             <1..588
                     /gene="azoR"
                     /locus_tag="OO564_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6654928.1"
                     /translation="LKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLAANPIPV
                     LDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNISTQLKNY
                     FDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTFLGFIGI
                     TDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(642..>1269)
                     /locus_tag="OO564_004047"
     CDS             complement(642..>1269)
                     /locus_tag="OO564_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6654929.1"
                     /translation="IVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTGKDS
                     QGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDV
                     TFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSR
                     SALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
BASE COUNT          322 a          367 c          289 g          291 t
ORIGIN      
        1 cttaaatcca gcatcctggc agggtactct cagtctaatc agttgtccga ttattttgtt
       61 gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct agctgcaaat
      121 ccgattccgg tactggatgg cgaactggtt ggcgctctgc gtccgagcga tgcgccgctg
      181 actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga gctgaaagcc
      241 cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac tcagttgaaa
      301 aactattttg acctggttgc acgcgcaggc gttactttcc gctataccga gaacggtccg
      361 gaaggtctgg taacgggtaa aaaagccatc gttattacca gccgcggtgg gatccacaaa
      421 gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt tatcggcatt
      481 accgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat ggcagcgaaa
      541 gcacagtctg acgcaaaagc agccatcgac agcattgttg ctgcataacc ggacacggtc
      601 ttgatgtgga ctgtagatat tcagtccaca tctcaatcca cttacctgtt ttcccataat
      661 ttcaacatcg ctttgtgctc ttccttaagc acaatatgcg agcgtctggc tcgaataaag
      721 ctaatcgctt catcaactgt tttacagtgt ccgtaacata acagccatgc cgccactacc
      781 agcgcactgc gcgataatcc caatgcgcaa tggaccagaa cgctgccttg ctcttcgcgt
      841 aatgtttcca gcatcgccac ggcctgtcgg agctccccct cttccggaac caccagatcc
      901 aacatcggta cacagaaata gagtcgatct tttgtcgctc gtccacgagg gaattcaaag
      961 gtgacgtcca gaaccgcatt ctgtgccgga atatgtcgtg gaaacgccct taaataaaca
     1021 ccagcagtca ttttgctcac cggctccagg cgacgacaaa accaacgcat agatagccac
     1081 atcccgatgc gccagggcaa tgtcagccag taaacggcgg gcgggagttt cccctgacta
     1141 tctttccctg ttgttatcgc gccaagccca ccgcacccac ggccaacaat gagtagcgat
     1201 aatactggcc aacataacca gactgaccac cataactgaa ccatcatcat tagctccatc
     1261 aacacaatg
//
LOCUS       DAJTYI010000264         1265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYI010000264 DAJTYI010000000
VERSION     DAJTYI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..671
                     /locus_tag="OO564_004048"
     CDS             <1..671
                     /locus_tag="OO564_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6654930.1"
                     /translation="MARDSVLLLEKLGCRVNFPEKQGCCGQPAINSGYIKEAIPGMKN
                     LIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAEKVAARMQDLTSFIVNKL
                     GVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGLELLTFAEQDTCCGFGGT
                     FSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISGRLQREGQKVKVMHIAEV
                     LMSR"
     gene            682..>1265
                     /locus_tag="OO564_004049"
     CDS             682..>1265
                     /locus_tag="OO564_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HCR6654931.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIRE"
BASE COUNT          347 a          276 c          355 g          287 t
ORIGIN      
        1 gaatggcacg agactccgtg ctgctactgg aaaaactcgg ctgtcgcgta aatttcccgg
       61 agaaacaggg atgctgcggt cagcctgcga tcaatagcgg ttatatcaaa gaagcgattc
      121 cagggatgaa aaatctgatc gccgcactgg aggataacga cgatcccatt atttcaccgg
      181 ctggctcttg cacctatgcc gtaaaaagtt acccgacgta tctggcggat gaacctgaat
      241 gggcatcacg tgccgaaaag gttgccgcgc gtatgcagga tctcacctct tttattgtta
      301 ataaattagg ggtagtcgat gtaggtgcca gtttgcaagg gagagctgtg tatcacccat
      361 cttgtagcct ggcccgtaag ctgggagtga aggacgagcc acttacgctg ctgaaaaatg
      421 tgcgtggact ggagctgttg acctttgctg aacaggatac ctgctgcgga tttggcggca
      481 cgttctcggt caaaatggcc gaaatatccg gcgagatggt gaaagaaaag gttgtgcacc
      541 tgatggaagt ccgccctgag tatttaattg gtgctgacgt gagttgcctg ctgaacatca
      601 gtgggcgatt acaacgggaa gggcagaaag tcaaagtgat gcatattgct gaagtgttga
      661 tgagccgctg aggatataaa gatgtcgatc aaaaccagta atacagattt taagacacgc
      721 atccgtcagc aaattgaaga tccgatcatg cgcaaagcgg tggcaaacgc gcagcagcgt
      781 attggggcaa atcggcaaaa aatggtcgat gaattggggc actgggagga gtggcgcgat
      841 cgggccgccc agatacgtga tcatgttttg agtaatctcg acgcttatct gtaccagctc
      901 tcagaaaaag tgacgcaaaa cggcggtcac gtctattttg caaaaaccaa agaagacgct
      961 acccgctaca ttttacaggt tgcccaacgc aaaaatgccc ggaaggtggt gaaatctaaa
     1021 tcgatggtga ccgaagagat tggtgtcaat catgtgttgc aggatgctgg cattcaggtg
     1081 attgaaaccg atctgggtga atatattctc cagctggatc aagatccgcc atctcatgtt
     1141 gtggtcccgg caattcataa agatcgccat cagatccgtc gagtgctaca cgaacgtctg
     1201 ggctatgagg ggccggaaac gcctgaagcg atgaccttat tcatccggca aaaaatccgt
     1261 gaaga
//
LOCUS       DAJTYI010000265         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYI010000265 DAJTYI010000000
VERSION     DAJTYI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            66..1259
                     /locus_tag="OO564_004050"
     CDS             66..1259
                     /locus_tag="OO564_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6654932.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DAJTYI010000266         1247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYI010000266 DAJTYI010000000
VERSION     DAJTYI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            210..1034
                     /locus_tag="OO564_004051"
     CDS             210..1034
                     /locus_tag="OO564_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6654933.1"
                     /translation="MVFITIFTSCYVSSKAYKQLSKSTRLYSDNHMWTKWSIYSIHGS
                     NGRDKYTYTKTLIYKKITTHEYVFIAICLIQFISIIMTSSETRVDMVLGANFATLCKI
                     LTTKVFLGLWANGDFLSPLWNHDSICIAITMICLSITIFTFIVSNYPMRSAIVFAILT
                     LTFSLAKPMLSSTSEQWPLLIHGGGRYSVIPTIIWYSVLVYFLNYISHLHFKFLPWML
                     YTTSLISCLYFFNLTPLPDYGWKEQVKKFESLAPGESYSFKFNPAGWTMTLIKKQP"
     gene            complement(1158..>1247)
                     /locus_tag="OO564_004052"
     CDS             complement(1158..>1247)
                     /locus_tag="OO564_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6654934.1"
                     /translation="LLAWKKYRVLLMRVDIKNTEWPKKPEENK"
BASE COUNT          374 a          245 c          182 g          446 t
ORIGIN      
        1 cttattgcct ccatatcatt aaattttaat ttaatgtatg cccctttaat ttttaacatc
       61 tccgcaatta ttgttagggc attgcttgta tctttcatac tctctggaag atttagttac
      121 gtaaacataa caccaagaat atttactgct ttgtttatta taataatgcc ggaagtatca
      181 gaagtgcacg ccaatgttac taatgattat tggtatttat cactatattt acttcttgtt
      241 atgttagctc caaagcctac aaacaattat caaaaagcac acgattatat agcgataatc
      301 atatgtggac taagtggtcc atttatagta ttcatggctc caatggtagg gataaatata
      361 cttatacaaa aacgcttatc tacaaaaaaa taaccactca cgaatacgtt ttcattgcca
      421 tttgtttgat tcaatttatt tctattatta tgacatcctc tgagaccaga gttgatatgg
      481 ttcttggagc taatttcgca acactttgca aaatattaac aacaaaggtt tttctgggtt
      541 tatgggctaa tggtgacttt ttatcacctt tatggaatca tgattcaatt tgcattgcaa
      601 ttacaatgat ttgtctttcc atcactattt ttacatttat tgtttccaat taccccatgc
      661 gctcggcaat agttttcgca atactaacat taacgttttc actagcaaaa ccaatgcttt
      721 catctacaag tgagcaatgg ccattattaa ttcatggcgg aggaagatat tctgtcatac
      781 caacaatcat atggtactct gtattggttt acttcttaaa ctatatttcc cacttgcact
      841 ttaaattcct accatggatg ttatatacaa catcgttaat ctcatgttta tattttttca
      901 atcttacccc actcccagat tacgggtgga aagagcaagt taaaaaattt gaaagcttag
      961 caccaggaga atcgtattct ttcaagttca accctgcagg atggacgatg acattaataa
     1021 aaaaacagcc ttaatgttga atggtagtta cttaatgtta ttgatctaag ttttgcatca
     1081 ataacccccc ccgaaactta ggggggggca tctgcagtta taattaacgg cctctttctt
     1141 catccgataa cgccccctta tttattttcc tctggttttt tcggccactc tgtatttttt
     1201 atatcaacac gcatcaatag cacccgatat tttttccatg ccagtaa
//
LOCUS       DAJTYI010000267         1246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYI010000267 DAJTYI010000000
VERSION     DAJTYI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(62..529)
                     /locus_tag="OO564_004053"
     CDS             complement(62..529)
                     /locus_tag="OO564_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6654935.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFPLTYPVFPLFC"
     gene            complement(595..834)
                     /locus_tag="OO564_004054"
     CDS             complement(595..834)
                     /locus_tag="OO564_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6654936.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(969..>1246)
                     /locus_tag="OO564_004055"
     CDS             complement(969..>1246)
                     /locus_tag="OO564_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6654937.1"
                     /translation="CENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELL
                     PIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          415 a          268 c          219 g          344 t
ORIGIN      
        1 ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct gtcctaaaaa
       61 attaacaaaa caatgggaaa acaggatatg taagcggaaa agccaatgaa attggtacag
      121 cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct gccagaacaa
      181 ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt tcatcaatag
      241 ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct gcggaaaaaa
      301 gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt tgatgctgta
      361 aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg ctaataattc
      421 tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat aaaagatcga
      481 ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag gttgctgcca
      541 gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta taagttacgg
      601 attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt cgaatgcatc
      661 tttaattgct cccaggaagc ctgagttctg atcgccaaag atgaaagcaa gcagagttgc
      721 catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat ccttcgcgaa
      781 acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat tcatgttttc
      841 ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac cggtatccca
      901 tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa aaaaaattac
      961 atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag cgtcgtcagc
     1021 cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt cgttctatac
     1081 cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga gaattcctgg
     1141 ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc gctatcgttg
     1201 gtatagctac tactgtcttc atcactgtca cgctcattct cacaga
//
LOCUS       DAJTYI010000268         1233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYI010000268 DAJTYI010000000
VERSION     DAJTYI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..258
                     /locus_tag="OO564_004056"
     CDS             <1..258
                     /locus_tag="OO564_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR6654938.1"
                     /translation="SRIHITSFLDANGSLNIDIASPGMKINEPEKLFRRFWRGDNSRH
                     SVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQRN"
     gene            complement(366..1217)
                     /gene="hchA"
                     /locus_tag="OO564_004057"
     CDS             complement(366..1217)
                     /gene="hchA"
                     /locus_tag="OO564_004057"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6654939.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT          322 a          283 c          293 g          335 t
ORIGIN      
        1 tcgcgtattc atataaccag ttttcttgat gccaacggct ctcttaatat tgatatcgcc
       61 agtcctggaa tgaaaattaa tgagcctgaa aaactcttcc gtagattttg gcggggagat
      121 aattcgcgtc attccgtagg tcagggactt ggcctttctt tagtcaaagc gattgccgaa
      181 ttacatggag gaagtgctac gtatcactat ctcagtaagc ataacgtgtt ccgaattacg
      241 ttaccgcaaa gaaattaaca tcgggcttca gtacgccata agttaaccgt gaaaaaacgc
      301 agccacgtcg tgagtattaa cgtggctgcg attgattatg cgcttacatc acaatgtaac
      361 agggattaac tcgcgtaagc tgccagcatt tcctgcgccg ccagtttacc caacgcattc
      421 gctgcaaaag gactgtcgcc ggtgagacgt ttacggtcct tatgtactcg cccggtgatg
      481 tcgtcattaa tgatgttcat gcccattttc ttcaactctt cgccgaagta ccaggtgaga
      541 tgacccggca tatagccaat atctggcgtt tgtttgtctg cggcgtctgg gaatgcgcaa
      601 atagaataac cattcagtgg gttatcgctg tggcgaagtg ccagaaaagc cgccggaccg
      661 tggcaaaggg agataacaaa acggtcattt ttgattgccc actgtaaagc ggcagccacg
      721 tcctggcttt ctggtaaacc aataagtgcg ccatgcccac caggaacaaa gattgctgca
      781 tattcgctat cagcgttgag gctggcgaca acatccgcga gtttcttcgg attacggaat
      841 aacgatttat gctgttcaaa gaatggcatt actttttcat ctttgtgcgg catagcccag
      901 tattcaaact tggtcatcag accggaaatg gtcgccactt cgaattcgaa acctgcagca
      961 tggagatgat acaacggcag caacgtttca atcggatggt taccggtcga gaacagtttt
     1021 ccgttatcgg tcggcaaata acgttcgtcc gcggcgatca ccagaatttt atgtttaccg
     1081 cgatacggtt ttggatagtc cacgccatca agatcagaga cgggactcgt atattggcta
     1141 agcgaatatt ctgaagggaa gaacgcatta tcttcagcaa tatcgacctg cgggttttta
     1201 cttgtttgaa cagtcatagt gtattcctta ttg
//
LOCUS       DAJTYI010000269         1221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYI010000269 DAJTYI010000000
VERSION     DAJTYI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            373..>1221
                     /gene="ipaH1.4"
                     /locus_tag="OO564_004058"
     CDS             373..>1221
                     /gene="ipaH1.4"
                     /locus_tag="OO564_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR6654940.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          414 a          252 c          206 g          349 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcggtaat tctatttcgg agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttcaccag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agcctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacgctg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcatt cccccctttc cagataagct tgaagggctg
      961 gctctggcta ataattttat agaacaacta ccggaattac cttttagtat gaacagggct
     1021 gtgctaatga ataataatct gacaacactt ccggaaagtg tcctgagatt agctcagaat
     1081 gccttcgtaa atgttgcagg taatccattg tctggccata ccatgcgtac actacaacaa
     1141 ataaccaccg gaccagatta ttctggtcct caaatatttt tctctatggg aaattctgcc
     1201 acaatttccg ctccagaaca c
//
LOCUS       DAJTYI010000270         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYI010000270 DAJTYI010000000
VERSION     DAJTYI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.275"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(177..1163)
                     /locus_tag="OO564_004059"
     CDS             complement(177..1163)
                     /locus_tag="OO564_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654941.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKTELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          427 a          280 c          218 g          286 t
ORIGIN      
        1 cgtacgaaag tacgggcttt tttttcgtat attgtacaca ccgggtgccc ccgcccacgt
       61 ctgttagggc gaggggaaat tgtgcacatc aactgtctgg ttactcagtc aacaatcaaa
      121 atgcgcaaga tagtacagaa catatatgcg ttatctggtc acttcctgta acttcctcac
      181 ttattaaaat gcccccaacg gaaatacatg tcacatgcca tcataaaatc gttatgccag
      241 aggaatcgac aatggaaaaa atagtaaatt aagcacggta catagcagag taaaaaatag
      301 gcttcaaacc aaatccgcca ttccacaagc gttgccaata ccgacaccag cgtaatccac
      361 atcatttcaa taaccagcaa tatgaataac cgaagccaaa gcggcttggc aaaaaaggtc
      421 gacaacgtat tttcactgga gaaaaacaga tcgatttcct gcaaaaaaaa cctgcccaga
      481 aaagcgtacc gcttggagaa gcgcagaaaa aacagattaa tcgccaacac tacgccacaa
      541 agaatgagca atgcaattat cgatttaaac gttaaccact gcccctctgt cacaaaagaa
      601 tcactgttac gcaacgggct gatagtaagc aggttaacaa ccgttgagat gcacaccgaa
      661 agcagcacat agcgagtcag acttttatat tttacttttt tcgctactga aatagcgacc
      721 tgcttgacat aactggtgcg gatcatccga aacagcagaa agatgaccaa cggcccaagt
      781 aacgaaatca ggaacagcgc atataacgag ggtagaagct ggtatagccc ccaactcatt
      841 gcactcaaca tgattaacga cagaaatcct gccaccagag gttgtgtaag aaataattct
      901 gttttgtggt tctgcttttt cttaaaccac aaaaaatcga tattcttcgt gacgcattta
      961 taagcccagt acgccttgag atcaaaaata aaattataca gcaccagggc cagaatccca
     1021 ctaactacac caatatcctc tgatggaaaa gtaaaccctg catttttcca ataaaagcct
     1081 atcagaataa gtccaatagc ataaatgtat atcagatttc gtttatcctg aaagcgaata
     1141 aaagttaaat aatcaggaat catttgaata cctttgcatc actaaatgct ttacgacaat
     1201 gcataactcg t
//
LOCUS       DAJTYI010000271         1193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYI010000271 DAJTYI010000000
VERSION     DAJTYI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(65..>1193)
                     /locus_tag="OO564_004060"
     CDS             complement(65..>1193)
                     /locus_tag="OO564_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6654942.1"
                     /translation="FTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLI
                     IAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVA
                     VVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCV
                     DIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASL
                     EKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRR
                     VQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKR
                     PWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
BASE COUNT          328 a          317 c          302 g          246 t
ORIGIN      
        1 ttttacatgg tgattaacca tgaaaacaaa aacgccccct tatgaagaaa ggaggcgtct
       61 ggcgttagat ttcgacctga gtacccagtt cgataaccct gtttggcggg atttcaaatt
      121 gatctggtgc acgcagcgca ttacgttgca gcagcaagta cagcttgccg cgcagacgca
      181 aataccacgg gcgtttgccg aggatcaacg actcatgcga cataaagaag gaggtttcca
      241 tcatccggca acttaatcct tccagaccgc agcggtggaa aacttcttcc acgtttggcg
      301 tttctcgcca accataactt gccaccacgc gccagaaagt gggcgacagt tgttcaatct
      361 gtacccgacg gacgttatgg acatatggcg cgtcttcggt gcgcagagtt aacaaaatca
      421 cccgctcatg caataccttg ttatgtttaa ggttatgcat cagcgcaaag ggaatgacgt
      481 tgattgcacg cgacatatac accgcggtcc cgggcacgcg aacaggcggt gatttctcca
      541 gcgaagcaat catcgcttcc agagagttac catgttcatg catccgccgc agcaagcgga
      601 aacgctcgct cttccaggtg gtcatcacga taaacatcac agtaccgagg ctcaatggca
      661 accagccgcc ggagagcagt ttatcgaggt tagcggtgaa caatggaata tcgacacaaa
      721 ggaaagcaat caggatcagc gcaacaaaat acttattcca gtgccagttc tgacgtgcca
      781 cggtagtcga gagaatagac gtcagtacca tggttccggt caccgcaatc ccgtacgccg
      841 ccgccaggtt gctggagtgc tcaaagctga caatcacaat cacgaccgcg acatagagca
      901 tccagttcac aaacggaata tagatttgcc ctgactccat ttcggaggtg tgaataatgc
      961 gcatcggcga caaatatccc agacgtaccg cctgacgcgt caatgagaag acgccagaga
     1021 taaccgcctg cgaggcaatt accgtcgcca gtgcggcgat gatcagcagt gggatcagcg
     1081 cccagtccgg tgccaacagg aagaacgggt tcttaatcgc ttccgggttc tttaacaaca
     1141 acgctccctg accgaagtaa ttaagggtca aggaaggtaa tacgacggtg aac
//
LOCUS       DAJTYI010000272         1191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYI010000272 DAJTYI010000000
VERSION     DAJTYI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..122
                     /gene="kil"
                     /locus_tag="OO564_004061"
     CDS             <1..122
                     /gene="kil"
                     /locus_tag="OO564_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6654943.1"
                     /translation="NIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            122..292
                     /locus_tag="OO564_004062"
     CDS             122..292
                     /locus_tag="OO564_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654944.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAYEL"
     gene            367..642
                     /locus_tag="OO564_004063"
     CDS             367..642
                     /locus_tag="OO564_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654945.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            742..>1191
                     /locus_tag="OO564_004064"
     CDS             742..>1191
                     /locus_tag="OO564_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6654946.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEK"
BASE COUNT          413 a          235 c          254 g          289 t
ORIGIN      
        1 acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca tgcatttctg
       61 actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt
      121 aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg aagaaagcaa
      181 aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg aaatgaaaaa
      241 atattgtagt gaaatctgtg ccgaaaaaga ccagatggca tacgaacttt aattaactga
      301 ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag
      361 aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat tgttcacgag
      421 gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa agaaacccca
      481 ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa cgatggtgta
      541 aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa agccgaattt
      601 ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac
      661 actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag taattttaat
      721 ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact gtattacgaa
      781 gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta ctgggctgaa
      841 gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga tgcaggctac
      901 gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga tgacctcccg
      961 ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc
     1021 atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc cagcactgac
     1081 aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct cctgccagtt
     1141 tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa a
//
LOCUS       DAJTYI010000273         1188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYI010000273 DAJTYI010000000
VERSION     DAJTYI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(62..>1188)
                     /locus_tag="OO564_004065"
     CDS             complement(62..>1188)
                     /locus_tag="OO564_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6654947.1"
                     /translation="LGDATLNGRELSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGA
                     YWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVL
                     EDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLD
                     FTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRLFWMEDPTPAENQEC
                     FRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIGYIRTTITHAGGITGMRRIADFASL
                     YQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFEEGYM
                     HPGDEPGLGISFDEKLAAKYPYDPEYLPVARLEDGTLLNW"
BASE COUNT          310 a          325 c          272 g          281 t
ORIGIN      
        1 gctccttgag cggaattaat cgatttgatg actattcaga aaataattaa gcgaaccgtt
       61 attaccagtt caacaatgtg ccatcttcca gtcgtgcgac ggggagatac tccgggtcat
      121 acggatattt cgccgccagt ttctcatcga aagaaatacc cagccccggt tcatcgcctg
      181 ggtgcatata accttcctcg aagcgccagc tgtgcgggaa aacttcaagc atttgttcgg
      241 aatagcccat gtactcctga acgccaaaat ttggtaccca taggtcaaaa tgcagcgccg
      301 ccgcatgaca aacaggcgat aaatctgaag ggccatgcga accggtacgt acctgataaa
      361 gcgatgcaaa atcggcaatc cgccgcatcc cggtaatgcc gcctgcatgg gtgatcgtcg
      421 tgcgaatata gccaatcagc tgctcttcga ttaactgctt gcagtcccag atactgttaa
      481 acacttcacc gaccgcaatc ggcgtcacgg tatgctggcg aatcagacga aaacattcct
      541 ggttctcggc aggcgtcgga tcttccatcc agaacaggcg gtaatcctca atgctcttcc
      601 caaagcgcgc cgcttcgatg ggggtcaggc gatggtgcat gtcatgcaac agatgttcgt
      661 taaaaccgaa cttattacgc accgcttcga ataacttcgg cgtaaaatcg agatactttt
      721 ctgaagacca caactgctct tctggccaca ggcctttggt tgccggttca tacgccagtc
      781 ctttgccctt cgccaggcca taagtggttt gcatacccgg tacaccgcac tgcacacgaa
      841 tcgccttaaa gcccatctgc tggtgctttg catagtcttc aaggacttca tcaatcgtac
      901 gcccggtggt gtggcaataa accatcacgc cttcacgaga agcaccacca agcagttgat
      961 acaatggcat atttgccgct ttagctttga tatcccacaa cgccatatca atggcagaaa
     1021 tggctgacat cgtgacgggg ccacgtcgcc agtaagcacc tttataaaag aattgccaga
     1081 tatcttcgat acggctggca tcgcggccaa ttagttgtgg acaaagatga tctttcagat
     1141 aagaagcgac cgacaattcg cgaccattta acgtggcatc tcctaatc
//
LOCUS       DAJTYI010000274         1176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYI010000274 DAJTYI010000000
VERSION     DAJTYI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..890
                     /locus_tag="OO564_004066"
     CDS             <1..890
                     /locus_tag="OO564_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6654948.1"
                     /translation="PTDTIPVPKTLEQAVVAPLRDFFGRNNAWLILLLIVLYKLGDAF
                     AMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLIFGIL
                     QGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGTSAFVALLMTLCNKSFSATQFAL
                     LSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAAVPGLILLLVCRQTLEYTRVNDN
                     FISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDALDLTHFSFLPALLEVGVLVALS
                     GVVLGGLLDYLALRKTHLT"
BASE COUNT          226 a          281 c          287 g          382 t
ORIGIN      
        1 aaccaaccga caccattcct gtgcccaaaa cgctggaaca agcggttgtt gcacctctgc
       61 gagatttctt tggtcgcaat aatgcctggc ttattttgct tcttatcgtg ctgtataagc
      121 tgggcgacgc attcgccatg agcctgacaa ccacgttttt gattcgcggc gtcgggtttg
      181 atgcgggtga agtaggcgtg gtaaacaaaa cgcttggctt actggcgacc attgttggcg
      241 cattgtacgg tgggattttg atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg
      301 gcattttaca aggtgcgtct aacgctggtt actggctgct gtcgattact gataagcatc
      361 tctacagcat gggcgcagcc gtctttttcg aaaacctctg tggcgggatg ggcacatcag
      421 cctttgtcgc gctgttaatg acgctgtgta ataagtcatt ttccgctact caatttgccc
      481 tgctctcagc gctttccgct gtagggcgag tttatgtcgg ccccgtggcg ggttggtttg
      541 ttgaagcaca cggctggtcg acattctatc tattctccgt cgccgctgcc gtaccggggc
      601 ttattttgct gctggtttgc cgccagacgc ttgaatatac acgagtaaat gacaacttta
      661 tctcccgtac cgaatatccg gcaggttatg cctttgccat gtggacactg gcggcgggcg
      721 tcagcctgtt ggccgtgtgg ttacttctgt tgacgatgga cgcgctggat ttgacgcact
      781 tctctttcct gcctgctctg ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc
      841 ttggtggttt gctggattat ctggcgctac gaaaaacgca tctgacgtaa tctgtaaata
      901 ttatttagct gtagtaatca ctcgccgtaa ttattacggc taaataaaca tcagcatcgc
      961 ttattaatat ttgttctttt gtgcggctta gcgtttggaa attattggcg ccatttataa
     1021 attctatttt tcttacacga ttcagctaat gagtctttat tttctcacca cccagttgtc
     1081 actctaatga taattatttg ttaaataatt gttttatttc gcattggtta taccaattgc
     1141 ccgccaagac tccttctgca ctcccctttt gttata
//
LOCUS       DAJTYI010000275         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYI010000275 DAJTYI010000000
VERSION     DAJTYI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..296
                     /locus_tag="OO564_004067"
                     /pseudo
     CDS             1..296
                     /locus_tag="OO564_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            224..697
                     /locus_tag="OO564_004068"
                     /pseudo
     CDS             224..697
                     /locus_tag="OO564_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          290 a          286 c          348 g          235 t
ORIGIN      
        1 gataaacaca cgtcagcagt tccgtcagca ctgcgacagc gttgtccgca aggcttttac
       61 ccagtccagc gttacggtgc cccacgcctg acggatgaac tgtgtgctct ggttaccact
      121 ttaacgtaaa aaccgtggca gccagtctgc accgtcagga actgagcgca aaagcctccc
      181 agaagttcag cccgatcagt taccgtgcac acggcctgcc tgtatcagaa aatctgctga
      241 cgcaggattt ttacgccagt ggcccgaacc agaagtgggc gggagacatc acgtactatt
      301 acagctcacc gactgcgggg aaacacgggg cgccgggtta ttgaaggtga cctgtccagc
      361 tacttcgaca cagtacatca ccggctgctg atgaaagccg tctgccgcag gatcagtgat
      421 gcacgtttta tgagactgct gtggaaaacg ccgtgttgat gttggtctct tccgggcggt
      481 cagtgaaagt atgccacagg gcggtgtgat ctcgccgttg ctgtcgaaca tcatactgaa
      541 tgagttcgac cagtatctga ataaacgcta cctgagtgga aaagccagaa aggatcgctg
      601 gtactggaat cacagtatcc agcgaggccg aagtactgct gtcaaagaaa actggcaatg
      661 gaaacctgcc gttgcttact gttgctatgc cgatgactgt gtgccacgaa ggcgcgtcct
      721 ggggacgtga gtccctgtgc agccatgcag cacaagagat gagggaaggc ccctcggtgt
      781 cggcgtgcag gcgcaggcac caaaccatcc actgctgctg catttaagag atgtggtggt
      841 gagcgagatg ggaaaaggtc ggacgtgagc cgatcaggta tggttcgaag agacgaacga
      901 aagtgaaccg ctgatgatgt gtcgaaagca tatagatgat gtcaaaacaa tggtggagtc
      961 gttcctgtgg gatatgttta ctgcctgcgc ggcatccggc ataaaagcgg cgtgaagtcg
     1021 attcaggctc ttgtggggaa cgtggacctg tcaccccgat gtgaagggag aagcgcaagt
     1081 ggaagcccca tgagcgccag agtaccaatg cggggtacag ggacggagca ccaagcgatt
     1141 gcgcagtaaa catgcttga
//
LOCUS       DAJTYI010000276         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYI010000276 DAJTYI010000000
VERSION     DAJTYI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(138..>1096)
                     /locus_tag="OO564_004069"
     CDS             complement(138..>1096)
                     /locus_tag="OO564_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6654949.1"
                     /translation="AAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLREELANP
                     ATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVAKNLGA
                     SDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDVKRIWS
                     ALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKHMKELD
                     ALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMRLYESK
                     AARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
BASE COUNT          257 a          238 c          237 g          364 t
ORIGIN      
        1 atttaatttg ctccagatac aatgaatcgc cgcagcgtgg ctacggtgaa ttttgggcat
       61 aaaaaaaacc agccgaagct ggatagttgc gttggttatc tgtcagtagt tatgtgctta
      121 tttaattatt tatctatcta ttgttctgct gtaatttggc ttgaaagtat agggcgtatc
      181 gcatttgcag cattatttaa cgctctttca taagctgccg ttcctgcttt ggtgtttgcc
      241 aaacgaagaa gcatattcct tgcggctttg gactcataca aacgcatcat tgcaccaaaa
      301 ccagcctcaa gccccattga tacaccaaga gtcgcagttg cgccaatcgt ccttatcctg
      361 ttggcttgcg attgccccgt ctgagttact acatttgcgg tgtctgacct tgctgtttgc
      421 tgtagaactt catgaagagc atcaagctct ttcatgtgct ttccagaaaa aatagtgttg
      481 taaatttcac cgcctgactg agatttcagc ttattaactt cagtgatgaa cttggctgga
      541 gagtcaccgg ccttttccgc tattttgctg acgtaagctg cacgcatagc atctttccct
      601 ttatcatcca gtgcgctcca gattcgtttc acgtcagatg gttttctgct taatacaacg
      661 gtatttataa gttcaggact ggcttcactg cttgccttgt tgagcttgtt agcaatgttt
      721 ttattaagca ccttattata aacgtttgca taatcggaat ttgctttaag gtattttgct
      781 gcgtctgatg caccgaggtt tttagcaact gcgttacgaa ggtcttttga cattgcattc
      841 tctaccatat tggtagctgc ttttgcctgg ttggggaaga ccatagcatc tccctgaaca
      901 ttagatctaa atgctgttct gtgctgacgc aagagatcaa acgtaacatc caaatcagtt
      961 gcagggtttg ctaattcttc acgtaggtta cgcaaggatg taagcaggct ttgattggca
     1021 gacgtcccaa gccgttcctg tcttgcgatc gctgtattca gagcattcat ggtatttgtg
     1081 gtatcaactg cggcat
//
LOCUS       DAJTYI010000277         1076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYI010000277 DAJTYI010000000
VERSION     DAJTYI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            308..>1076
                     /gene="ipaH7.8"
                     /locus_tag="OO564_004070"
     CDS             308..>1076
                     /gene="ipaH7.8"
                     /locus_tag="OO564_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6654950.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          367 a          212 c          153 g          344 t
ORIGIN      
        1 cagtaatttt aatttgtcat tctataatag gaataaatga gcttgactaa aatatgatgt
       61 aaaacaaata tacttactcg gattacattt ccttgctcac tggaatgttg aattcagtgt
      121 tattattgac tgcagtcaca ataaaaaaga attccataga aaacctccat aaataaatta
      181 caacataact tctgttatgt gtaaaatgga aactattaaa acttaatatc ggaaatggta
      241 agtgaaattt gtataaatat acaattttaa atatttattc tcacaaatat aaggttgacc
      301 tagcattatg ttctctgtaa ataatacaca ctcatcagtt tcttgctccc cctctattaa
      361 ctcaaactca accagtaatg aacattatct gagaatcctg actgaatggg aaaagaactc
      421 ttctcccggg gaagagcgag gcattgcttt taacagactc tcccagtgct ttcagaatca
      481 agaagcagta ttaaatttat cagacctaaa tttgacgtct cttcccgaat taccaaagca
      541 tatttctgct ttgattgtag aaaataataa attaacatca ttgccaaagc tgcctgcatt
      601 tctcaaagaa cttaatgctg ataataacag gctttctgtg ataccagaac ttcctgagtc
      661 attaacaact ttaagtgttc gttctaatca actggaaaac cttcctgtgt tgccaaacca
      721 tttaacatca ttatttgttg aaaataacag gctatataac ttaccggctc ttcccgaaaa
      781 attgaaattt ttacatgttt attataacag gctgacaaca ttacccgact taccggataa
      841 actggaaatt ctctgtgctc agcgcaataa tctggttact tttcctcaat tttctgatag
      901 aaacaatatc agacaaaagg aatattattt tcattttaat cagataacca ctcttccgga
      961 gagtttttca caattagatt caagttacag gattaatatt tcagggaatc cattgtcgac
     1021 tcgcgttctg caatccctgc aaagattaac ctcttcgccg gactaccacg gcccac
//
LOCUS       DAJTYI010000278         1075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYI010000278 DAJTYI010000000
VERSION     DAJTYI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            350..538
                     /locus_tag="OO564_004071"
     CDS             350..538
                     /locus_tag="OO564_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654951.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLEPEA
                     FLDKYPRRLKSGKTRPPG"
     gene            635..814
                     /locus_tag="OO564_004072"
     CDS             635..814
                     /locus_tag="OO564_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654952.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            866..1060
                     /locus_tag="OO564_004073"
     CDS             866..1060
                     /locus_tag="OO564_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654953.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          368 a          248 c          216 g          243 t
ORIGIN      
        1 tccctttcat tcgcccaacc gataccatac tttatgccaa cagaaagaat gaatgaaccg
       61 cgcctaaaat cagcaaggac acatggcaca aagtgcgaca tgtctcacat ttaaccccag
      121 tgggggcaac aagtataaaa ccgttttttg attaagtggt cgaatatgaa aaggggaagg
      181 tttatgtgaa aaatacctgt gttttctttg aaaacacaca acagtatcag gttatttatg
      241 tttatacagt gcgttccaaa ctgacctgaa taattacgca agttgcaaca ataaaacatt
      301 tttgccgtag tgtgaggtac tccggtaaat tgctttccag gtgcagacaa tgactaacga
      361 agaactacag gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag tacaccagca
      421 acgagagtct ttgcttaacc catctccatt caccaggcca aaacgattgg agcctgaagc
      481 attcttggac aaatacccac ggcggcttaa atcaggcaaa accagaccac caggctgata
      541 gctcacagtt tgttctccga ttcaataaaa cggataagaa ccatcagcgc ggtagcgtaa
      601 ggcactacag cgcattgctt tacgagtaat aacaatggat ttatcaccag aagacgcaaa
      661 gtttttacga gaactgcaac accgtatgct cagagaagcc attatcgaac agcaaacggg
      721 cataagcgcc atagacccat tgcgatacaa acgggcctca tctcctttag ttgacgacaa
      781 ttccaatgcg gataaaacac gtccaccaca ctgattacat ttttcattag cacagtggtg
      841 tgatgcagca tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc tggagcaact
      901 gttacaggca cgccaacggg agtttgccac taaatgggga cgtgccgcgc tgccacatga
      961 acgcctaacc tattttccac ttgaagacct cattaaaaat aatggcaaaa aaatcaggtc
     1021 tgataagaaa cacttcttag aatgcaatga agacaaataa caacaatcta catat
//
LOCUS       DAJTYI010000279         1061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYI010000279 DAJTYI010000000
VERSION     DAJTYI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..197)
                     /locus_tag="OO564_004074"
     CDS             complement(<1..197)
                     /locus_tag="OO564_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654954.1"
                     /translation="MPVFHNTRLLVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRH"
     gene            complement(271..549)
                     /locus_tag="OO564_004075"
     CDS             complement(271..549)
                     /locus_tag="OO564_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654955.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(616..866)
                     /locus_tag="OO564_004076"
                     /pseudo
     CDS             complement(616..866)
                     /locus_tag="OO564_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          270 a          275 c          263 g          253 t
ORIGIN      
        1 atatggcgca acagccagtc agacaatgca ccagcaccac cagcagcacg aatcacccgt
       61 gcgttactga aaaacggcag caatgttttg tcttccacca gcggctggcg agcggcagga
      121 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag taaccgggta
      181 ttgtggaata ccggcatgga ttcacgaccg ggcttaagga ccaccagccc gagttccgga
      241 accagaacag gtcgaagtaa tacccgcgcg ttacctccag atccgttgct ggaatgtgcg
      301 ggacggacgc ggtggctgtt cggagtaagg gagcctgacg gagattatcc agtgacggta
      361 gtcgaggcta agggcttttt taacctcgca tccgcgcctg cggtaacact gaatgagcca
      421 ttcggcctgt tcttcagtgc atgagggatg ctggtaccag tctgacttaa atgcgtgaga
      481 ataccgctcg tgcgtgtggg caagaacggt cgaattatca tgattgtaat attttgcgtt
      541 gcgtgccatc ggttttctcc ggtggcacgg tgttactcag cgggagttca gccccgcgca
      601 agattgtaga tgagtttatt cttctgaaaa agcagaaaag ccagctttta ttccgatctc
      661 tttcaatgcc tgtaatgaag tgacaaactc accgtcgcgc aagataaatc cgtccgtcac
      721 tcgggcatcc acaaaattaa ttaacgcagc cccatttttt gcaaacacac aatgcggtaa
      781 tgactaacaa tatttccatt ttcaacgcac acagcataga ggccatcttc acaaaaaatt
      841 ttacgcagtt cttcgatgtt catcatcaga atccttccag ataagtagca ctcccctgtt
      901 cggggtccat cccttttatc cctgcgcgct acttaagtat ttttgattct attccggcac
      961 catccagaac ttcaaatgcg ttgaaaataa aaacaaaaac ccgccgaagc gggttaagtg
     1021 cgggtgcgtt gaggatgcct gccacatcag aggtggcgag g
//
LOCUS       DAJTYI010000280         1057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYI010000280 DAJTYI010000000
VERSION     DAJTYI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(150..1019)
                     /locus_tag="OO564_004077"
     CDS             complement(150..1019)
                     /locus_tag="OO564_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6654956.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          277 a          304 c          224 g          252 t
ORIGIN      
        1 ccaggaaata aaaaagagaa atgagtaact ggatgcaaaa cgatagtacg catttctgaa
       61 tggaatattc agtgccataa ttacctgcct gtcgttaaaa aattcacgtc ctatttagag
      121 ataagagcga cttcgccgtt tacttctcac tattccagtt cttgtcgaca tggcagcgct
      181 gtcattgccc ctttcgccgt tactgcaagc gctccgcaac gttgagcgag atcgataatt
      241 cgtcgcattt ctctctcatc tgtagataat cccgtagagg acagacctgt gagtaacccg
      301 gcaacgaacg catctcccgc ccccgtgcta tcgacacaat tcacagacat tccagcaaaa
      361 tggtgaactt gtcctcgata acagaccacc accccttctg cacctttagt caccaacagc
      421 atggcgatct catactcttt tgccagggcg catatatcct gatcgttctg tgtttttcca
      481 ctgataagtc gccattcttc ttccgagatc ttgacgacat ccgccagttg tagcgcctgc
      541 cgcaaacaca agcggagcaa atgctcgtct tgccatagat cttcacgaat attaggatcg
      601 aagctgacaa aacctccggc atgccggatc gccgtcatcg cagtaaatgc gctggtacgc
      661 gaaggctcgg cagacaacgc aattgaacag agatgtaacc attcgccatg tcgccagcag
      721 ggcaagtctg tcgtctctaa aaaaagatcg gcactggggc ggaccataaa cgtaaatgaa
      781 cgttcccctt gatcgttcag atcgacaagc accgtggatg tccggtgcca ttcatcttgc
      841 ttcagatacg tgatatcgac tccctcagtt agcagcgttc tttgcattaa cgccccaaaa
      901 ggatcatcac cgacccgacc tataaaccca cttattccgc ctaatctggc gattcccacc
      961 gcaacgttag ctggcgcgcc gccaggacaa ggcagtaggc gcccgtctga ttctggcaag
     1021 agatctacga ccgcatcccc taaaacccat actttgg
//
LOCUS       DAJTYI010000281         1051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYI010000281 DAJTYI010000000
VERSION     DAJTYI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>1051
                     /locus_tag="OO564_004078"
     CDS             <1..>1051
                     /locus_tag="OO564_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6654957.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEH
                     RHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKV
                     CHLLTSMTDAMRFPGGEMADLYSNRWEIELGYREIKQTMQLSRLTLRSKKPELVEQEL
                     WGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMR
                     DLASMGQLVKLPTRRG"
BASE COUNT          281 a          265 c          308 g          197 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttattactc actgggactg ttaaatggct ggagcctggc gggagaacac
      481 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      541 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      601 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      661 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      721 agtaatcgct gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc
      781 aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta
      841 ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac
      901 tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca
      961 ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga
     1021 caacttgtga aattaccgac gagaagggga a
//
LOCUS       DAJTYI010000282         1043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYI010000282 DAJTYI010000000
VERSION     DAJTYI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(20..532)
                     /locus_tag="OO564_004079"
     CDS             complement(20..532)
                     /locus_tag="OO564_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6654958.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            727..>1043
                     /locus_tag="OO564_004080"
     CDS             727..>1043
                     /locus_tag="OO564_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654959.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPG"
BASE COUNT          336 a          229 c          243 g          235 t
ORIGIN      
        1 agagcttttt atgggcagtt tagctaaaaa aaggcaacac gaagctaaaa ctaccaacat
       61 aaagtgctga ccagctagca gctgaaatca gactcactgt atacactttc actggatgaa
      121 gttgcaacat tcctgccacc aaaggagcaa tgtaacgcag cacggcgata aagcgcgaag
      181 tgaaaagtat tgacacagag ttattttgca actgaaggcg aacccgttct attgtattgg
      241 aacggctcga tattatacat gcaagttgag ggatatgccc aattattata ccaagatggt
      301 aattaacgat agtaccgcac catgcgccac tcatgaccgt gattccagct tcccatggtg
      361 aaagagtcgt cttgctgact gtaattacag tggccatcat aattgatgct ggtggcaata
      421 tcgccgaaat gagtaaagtg gattttgtga acgctattat aaaaagaagc ccccataggc
      481 gatatggatg aagtacaaaa tagttcatca aagcatttat ccactccatc aatgtctttc
      541 cttctgtgaa tttttctttg ctatttaacc gtgatacgac ttaatttagg cttaaacacc
      601 ttacatttac aaggtactcc tgtggggggc cctgccacgg agcgtcgcag gagtgagtga
      661 tgaggaagaa ttaaagcaac ctattgtgac aggcaacgat aacagtaaat cacaggaagc
      721 ataatcatgg caaaaccgga ctgggaggcc atcgaatcgg cataccgggc cggagtcctt
      781 agcctccgtg atataggcga gaaatacggc gttacagaag gggctatcag gaagagggcc
      841 aaaaagcttg gttgggcacg cagtggcggt acgcaggttt gcaaaaatgg tacgcaaaaa
      901 aggaaagtgc gtaccagcag aaagcctgcc attactggcc ttacacaaaa aagtacgcaa
      961 ctaaaaacag aatctacacc ggatacgaaa ccgatacgcg gaatgcgtac cgatcccccg
     1021 actaacccat tccaacccgg taa
//
LOCUS       DAJTYI010000283         1040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYI010000283 DAJTYI010000000
VERSION     DAJTYI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>1040
                     /locus_tag="OO564_004081"
     rRNA            <1..>1040
                     /locus_tag="OO564_004081"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          242 c          329 g          211 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac
//
LOCUS       DAJTYI010000284         1034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYI010000284 DAJTYI010000000
VERSION     DAJTYI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..464)
                     /locus_tag="OO564_004082"
     CDS             complement(<1..464)
                     /locus_tag="OO564_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6654960.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFL"
     gene            896..>1034
                     /locus_tag="OO564_004083"
     CDS             896..>1034
                     /locus_tag="OO564_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6654961.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSHDSSMLIDAVKNK
                     IT"
BASE COUNT          283 a          270 c          195 g          286 t
ORIGIN      
        1 tcaagaaatc gaacttgcct ttcaagatcc tcaatgcgtc ggtcttttga cagctccaat
       61 gctgatgccg ctttttctgg atcaactgat attgcaatgt ttcttttggt gccaatcttg
      121 agcgcgcgta aaccagcttc tccgcgctct tcataaacct tcagccacct ggctacagaa
      181 ccactaccag caagcataaa gtgagcagca gcctgattaa gggacatgtg ctgctcaatc
      241 acagctttca cgaccttaat acgcaactct ggatcagcac taacaccttt aggtttggga
      301 attaagcctt tttctccatg tttttcatag agggcaaccc atgtcctgac ctgggttcgg
      361 gggacaccaa aacgtgccga gatgatcctg taaccatcat cagtagtgaa gtagtgattc
      421 acgacttcaa ggcgcttttc aaaagggtat tttggctttg acatattagg ggctattcca
      481 tttcatcgtc caacaaaatg ggtgcagtac agggttgata gaaatatctc ctggggcttc
      541 atctttccag acctcagagt aaacctgaaa tcggaaagct tcaggcaccc gcagttatct
      601 tacccttcct caacaaaata gctgtttatt tatacagcaa atcctgtttc ggacttctca
      661 ctccccattt gatcaactga agctgaaccc gtttttttct gcatgcctca atcgtcaaaa
      721 ttcttcgcca ccagccagct ctgccataac ttgccacatt gaaaaagtag ccaaagtgct
      781 tcaggcacca tcagttcacc atcaccaaaa aaaagaaaca ttccagaact tccgcatctc
      841 gcccagccgc tccgacttct atactgaata gaaaacgtca acttaagaga aacctatgcc
      901 attacccgat tttcattttt ctgaaccttt taccctcggt attgaactgg aaatgcaggt
      961 ggttaatccg ccgggctatg acttaagcca tgactcttca atgctgattg acgcggttaa
     1021 aaataagatc acgg
//
LOCUS       DAJTYI010000285         1032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYI010000285 DAJTYI010000000
VERSION     DAJTYI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(56..>1032)
                     /gene="yiaK"
                     /locus_tag="OO564_004084"
     CDS             complement(56..>1032)
                     /gene="yiaK"
                     /locus_tag="OO564_004084"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6654962.1"
                     /translation="KAAFNRVLISRGVDSETADACAEMFARTTESGVYSHGVNRFPRF
                     IQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVA
                     LRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPI
                     TMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKG
                     SGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRI
                     MDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
BASE COUNT          235 a          271 c          285 g          241 t
ORIGIN      
        1 ggcaaacggc acggattggg ctgcgcgata tgtccaaaaa tcatgactta tctcctcata
       61 acgcctggat tttggcccac acgctgtcat caacagtgat gccgttacgg cggttttcgg
      121 ccagcagggt agtaaattcg tggccgggta agcggatggc ctggttttcg tcagcgcgtt
      181 cagcggtagt aacgtaatcc atgatacgtt gcagcttggc atcgcgggtg ggaccgtcga
      241 taagcttgtc cacttcaatg gcaataaaaa tttgtgaaat gccgtattcg tcgctgttgt
      301 cctgggtgac ttcggcaacc gatgcgccgt cggaaaggag agtagcgatc atatccagca
      361 caatcgacat gccagaacct ttccagtagc ccatcggcaa aatgcggcga ttcttctcga
      421 taacgccagg ttctttggtc aaattgccct catcatcaaa gccaccatcg accgggagct
      481 gacggcctgc caggcggtta acttctaaca tgccgtaaga gaacatcgac atcgacatat
      541 cgaccatggt gatcggcgtg gaaggaatgg cgacgatcag cgggttagtg ccgatgcgac
      601 actcttttgc gccccacggc ggcattacgg cgatggagtt ggtccagcaa atgccaatat
      661 agcctttttc cgccgcctgc cagccgtagc tgccgccgcg catccagtgg ttggcattac
      721 gaagtgccac cagaccaata ccgtgatcgg cagccagttc aatggcgcga tccatcatct
      781 ttttcgccgt caggttgccg atcgaacgct gggcatccca ctgttcaatt gcgccgaggc
      841 tggttatacg tttgggttgg gcatcaggaa tgatatcgcc gttttccagt tgttgaatga
      901 aacgagggaa acgattaacg ccgtgagaat aaacgccgga ttcggtggtg cgggcgaaca
      961 tctctgcaca ggcgtcagcc gtttcgctgt caacgccgcg tgaaattaag acccgattaa
     1021 aggctgcttt ta
//
LOCUS       DAJTYI010000286         1029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYI010000286 DAJTYI010000000
VERSION     DAJTYI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(445..>1029)
                     /locus_tag="OO564_004085"
     CDS             complement(445..>1029)
                     /locus_tag="OO564_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6654963.1"
                     /translation="GADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISPPKIEENVGD
                     FRLMSREVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNL
                     ALEGITSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFL
                     GGVQLIGIGVLGEYIGRIYIEVKNRPKYIIKDKK"
BASE COUNT          346 a          204 c          177 g          302 t
ORIGIN      
        1 tataagtaga gaataaccaa taaatgcaat agcacttggc cacagatagt tgaatgatcc
       61 tgttatccaa atcgcggcat tgaaaaaaac atcgcttggc atcataacaa tcagtaataa
      121 tgacatgatt gaagtgttta ttttatagta aaggttgttt tttctctgaa agatagagca
      181 aatgccaata attaatgatg aaaacattgc tgcattaatt aaagaccaag cataaaaatg
      241 attaataaga tttattaatg catattcaat ctatgttcgt gatgaccatg tgaaatatct
      301 atctatgagc cacttgatgt gatccggttc tgatattgat ttctcggaaa accaaatgtc
      361 atctgatact agtgtatatt gatttaaatg aagaatagtt aaaccaataa aaaagcaaaa
      421 gaaagcatat gtaatttgat ttaatcattt cttatcctta ataatatatt taggtctatt
      481 ttttacttca atatatattc tgcctatata ttctccgaga acaccaatcc cgatcagttg
      541 cacgccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
      601 aaagacaagg gtgtctataa tcatccatgc accatataaa aacgaaatgc ttgcaacaaa
      661 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
      721 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
      781 tacatattca acgacatctg tctgaccacc cacccagctc agtatgcctt tcatgaaaag
      841 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
      901 tccgacattt tcctcgatct ttggggggct tattttgttg tgtaacttat agaaccactc
      961 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttcg caagcaccat
     1021 gtcagcacc
//
LOCUS       DAJTYI010000287          982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYI010000287 DAJTYI010000000
VERSION     DAJTYI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(19..822)
                     /gene="dkgB"
                     /locus_tag="OO564_004086"
     CDS             complement(19..822)
                     /gene="dkgB"
                     /locus_tag="OO564_004086"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6654964.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          220 g          284 t
ORIGIN      
        1 aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc taaccaggcg
       61 gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct gtaaattttg
      121 tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta ctgagtaacc
      181 ttcccccata gcccacgcca gaatcacttg tgccggaatc gcattgtgtt tagctgcaat
      241 acgagcaata acctcatctt tcagtgcctt accataagcc agcgtcatat aggaagtgat
      301 atgaataccg tgctgtttag cccaggcaac cactttacgg ttttgcagat aaggagagag
      361 ttcaatctgg ttggtagcga tgttttcagc gccaacagca gcaatcgctt tttccatcaa
      421 tgggatcgtg aagttggaaa taccgatctc acgcgtcagc ccttgttttt tggcttccag
      481 cagcgccttc ataaactctt caacagagac ttcatcgttt ggtgacggcc agtggattag
      541 cgtcagatca acataatcgg tatgcaattt ttgcaggctc tctttcagac tcgggatcaa
      601 tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt catgacgtgg
      661 cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga tttgtgcggt
      721 atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc
      781 tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc tcttttaatg
      841 ctattttcgt taactgaggt gattatgggc ttcagactgt tgcggaaaaa gagcgtaaaa
      901 cgcagaggat ttttgcgatt ctagcaataa tagatatcca gacgcaaaaa aacatcgagc
      961 gttaactcga ggcttcttaa ta
//
LOCUS       DAJTYI010000288          951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYI010000288 DAJTYI010000000
VERSION     DAJTYI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..469
                     /locus_tag="OO564_004087"
     CDS             <1..469
                     /locus_tag="OO564_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6654965.1"
                     /translation="MPVVIIPAEYLGKTGYELPAELDADKALLARIESIRLQAGKAMG
                     LGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAIAISSSCALEGTVTR
                     QIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRTTRKIFSGEVYLP"
     gene            545..>951
                     /locus_tag="OO564_004088"
     CDS             545..>951
                     /locus_tag="OO564_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6654966.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTMLGLGYVTVFLDL"
BASE COUNT          234 a          204 c          234 g          279 t
ORIGIN      
        1 gatgccagtc gtcattattc cggctgaata tctgggaaaa acaggttatg aattaccggc
       61 ggaactggat gccgacaaag cattattagc ccgcattgaa tctatccggc tacaagcggg
      121 taaagcaatg ggcttaggtg atgtcagtaa tatggttatc cctaaacctg tgcttatttc
      181 tccagcgcag aaaggcgggg caattaatgt gcgttatttt atgccgcatt cttgccatcg
      241 cgcgctggcg ataaccggtg ctattgctat ttccagtagt tgtgcattgg aaggcaccgt
      301 cacccgacaa atcgtccctt ctgtaggata tggcaatatc aatattgaac accccagtgg
      361 tgcgctcgac gttcatttaa gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt
      421 tattcggaca accagaaaaa tattttccgg tgaagtttat cttccctgaa aaaattcgtt
      481 gtcaggataa ggacaatcaa taaaggactt ctgtatgagt catacagaaa gaacaggatt
      541 ttaaatgaat aagaaatcgt tatggaagct aattctgata ttagcgatcc catgtattat
      601 tggttttatg ccagctccgg caggattaag cgaactggcg tgggtgcttt ttggtattta
      661 cctggcggcc attgtgggtc tggttatcaa gcctttcccg gaacctgtcg tactgttaat
      721 tgccgttgct gcctcaatgg tggtggtcgg taacttatcc gacggtgcgt ttaagaccac
      781 cgccgtatta agcggttact cttcaggtac tacctggctg gtgttctcgg cgtttacctt
      841 aagcgccgca tttgtgacca ccggtttagg taaacgtatt gcctatctgc tgattggtaa
      901 aatcggtaac accatgctgg gtctgggtta cgttacggta ttcctcgatc t
//
LOCUS       DAJTYI010000289          943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYI010000289 DAJTYI010000000
VERSION     DAJTYI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..943
                     /locus_tag="OO564_004089"
                     /pseudo
     CDS             1..943
                     /locus_tag="OO564_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          256 a          237 c          215 g          235 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga gattccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agc
//
LOCUS       DAJTYI010000290          936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYI010000290 DAJTYI010000000
VERSION     DAJTYI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(77..767)
                     /locus_tag="OO564_004090"
                     /pseudo
     CDS             complement(77..767)
                     /locus_tag="OO564_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          239 a          218 c          241 g          238 t
ORIGIN      
        1 atggtaaagg cgttcaacta tatcctgaat cactggaatg cactgaatga gttttaccgg
       61 gtagtacttc caatcattag aatatgtgct tttcaatgaa ggaaccgatg actttttcgt
      121 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggggc
      181 ggagggtcag gttgttacgt tcatacgttg cgtgaagatt ttgccggtca ggtgtttctc
      241 ctccggcact tctctggcgt agctgcccca gtcatcgctg gtcaccatgc cgatacagaa
      301 cggagtcaac agggctaaca gttcccgaca ggtctcatca ttccgtggcc cgcaagtgta
      361 agccaacaca ccgccagttt tagtgttgta cgcataccag ggccagtgtt gttgcgcctt
      421 gctgccaacg aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc
      481 gactggcgca gatgttatcc gccggggcga gagcttttta atgtccgtat gacggtattg
      541 atgccgattt tcagtgttct ggcggtataa cagccccctg cgccgttatg tgccatttca
      601 acgatctgct ccttaacgcc gagtattctg gcttcataag agtaagtgag ctggaaaaca
      661 cgtttacatg agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa
      721 acttcatcgg aattacagcg cggacaatga acagtaacgg tagtcatgga aaacctcaaa
      781 taccagtaga ttaccagaaa cttctatcga ttgaagacat taccttttgc cgcaataggt
      841 ggggtaacat tggtgaaaac gcattacgcc gggtggcagt tggaaaaaaa ttatcttttt
      901 tcggctctga tcgtggtgga gaaaatgcgg caataa
//
LOCUS       DAJTYI010000291          926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYI010000291 DAJTYI010000000
VERSION     DAJTYI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            24..479
                     /locus_tag="OO564_004091"
     CDS             24..479
                     /locus_tag="OO564_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6654967.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            482..>926
                     /locus_tag="OO564_004092"
     CDS             482..>926
                     /locus_tag="OO564_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6654968.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANV"
BASE COUNT          261 a          226 c          238 g          201 t
ORIGIN      
        1 atcaagataa tcgcggagca gtgatgattc agttcaaacc aacgcgaaac atcgacctga
       61 tcgaagcagt aggaaatcac actgacatta tcgccgggag caacaacggc gatggatacg
      121 actacaagcc tgaatgccgt tactttgagg ttaacgtgca cgggcagttc ggcggaattg
      181 tttactatca ggagattcag ccgctaacct ttgattgcca cgccatgtac ctgccagaga
      241 ttcgtggatt cagcaaggaa atcgggctgg cgttctggcg atacattctg actaacacca
      301 ccgttcagtg cgtcacatcg ttcgctgcgc gcaaattccg ccacggtcag atgtactgcg
      361 caatgattgg ccttaagcgt gtaggaacca tcaagaaata cttcaaaggc gtggatgacg
      421 tgacgtttta cagcgccaca cgcgaagaac taatcgactt cctgaatcac gggagatagc
      481 catgttatat gcatttaagc tgggcagaaa actgcgcggc gaggaacctt attgccctga
      541 aaaaggcggg aaaggtggca gctctgataa aagcgcaaag tatgcagcag aagctcagaa
      601 gtatgccgca gacctgcaaa atcagcagtg gcagacgatc atgaaaaacc ttgctccgtt
      661 cacgcctctt gcggagcagt atgttaacca gcttcagaac ctttccagtt tagaaggtca
      721 ggggcaggca cttaatcagt attacaactc tcagcagtat aaagaccttg caggtcaggc
      781 tcgttaccag agtcttgctg ctgcggaggc gacgggtgga cttggttcga cagccacaag
      841 caatcaactg gctacgatcg cgccgactct cggtcagtct tggttatcaa accagatgag
      901 caattacaac aatctggcaa acgttg
//
LOCUS       DAJTYI010000292          926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYI010000292 DAJTYI010000000
VERSION     DAJTYI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>926)
                     /gene="cusS"
                     /locus_tag="OO564_004093"
     CDS             complement(<1..>926)
                     /gene="cusS"
                     /locus_tag="OO564_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6654969.1"
                     /translation="FAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNV
                     LISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHME
                     HSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIV
                     LLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFT
                     RQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDM
                     LFLAQADNNQLIPEKKMLNLADEVGKVFDFFEA"
BASE COUNT          219 a          226 c          243 g          238 t
ORIGIN      
        1 gcctcgaaaa aatcgaacac tttgccgact tcatccgcca ggttgagcat tttcttttcg
       61 gggattagct ggttgttatc ggcctgagcg agaaacagca tatcgctgac cattttcgcc
      121 attcgcgtca gctcttcgag attagagtag agcacatctt ccagctcctt ctggctgcgc
      181 gactggctga gggcgatttc cgtttgcgta atgagattag taattggtgt gcgaatttcg
      241 tgggcgatat ccgctgagaa attggactgg cgagtaaaga catcctcaat acgctcgatc
      301 atatggttga acgacagtac cagctgttcc agctcaatcg gcacggtctg cgggtcaagg
      361 cgaacgtcga gatctttcga ggtgatattc tggatttgac ggctgacgct gcggatcggc
      421 gcgtgacctt tatgtaccgc caacagcacg ataaagacga tcaggatgct gattaccgat
      481 gcggtcataa taagtttatt catcaaatca tttatgtaat gaagatgaaa atcgatcgac
      541 agcgcgatgt agagcgtata aatcggtttg ccgtccacca acgggccaac cggcaagtta
      601 atcatccgcc agttgctgtg ttccatatgc ccgtgaccgt ggcctggcat cataatcgtc
      661 gggccggaaa ggagatacac ctcgccaccc cgcgcgtctt tatcgggtat ggcatcacgt
      721 gcaaactcgc ggatatcagg cgcaccgggg gagtgataca ccgttttgcc gtggctatct
      781 gccagggaaa tcaacacgtt ggaataacca ctgacgatat cttccagcgt cattaagcgt
      841 cgggcttgcg tttcgtcagg gtgatttagt acccgttcaa gggtggcgct aatctctttt
      901 aaatcattaa tgtcctgctc ggcaaa
//
LOCUS       DAJTYI010000293          925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYI010000293 DAJTYI010000000
VERSION     DAJTYI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(15..>925)
                     /gene="yjfF"
                     /locus_tag="OO564_004094"
     CDS             complement(15..>925)
                     /gene="yjfF"
                     /locus_tag="OO564_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6654970.1"
                     /translation="ASTRVICNILTDNAFLGIIAVGMTFVILSGGIDLSVGSVIAFTG
                     VFLAKVVGDFGLSPLLAFPLVLVMGCAFGAFMGLLIDALKIPAFIITLAGMFFLRGVS
                     YLVSEESIPINHPIYDTLSSLAWKIPGGGRLSAMGLLMLAVVVIGIFLAHRTRFGNQV
                     YAIGGNATSANLMGISTRSTTIRIYMLSTGLATLAGIVFSIYTQAGYALAGVGVELDA
                     IASVVIGGTLLSGGVGTVLGTLFGVAIQGLIQTYINFDGTLSSWWTKIAIGILLFIFI
                     ALQRGLTVLWENRQSSPVTRVNIAQR"
BASE COUNT          266 a          272 c          225 g          162 t
ORIGIN      
        1 acggagaggc atttttatcg ctgcgcaatg ttgactcttg tcactggcga actctgacga
       61 ttctcccaca gcaccgttaa cccacgctgt aatgctataa aaataaacaa caaaataccg
      121 atggcgattt tcgtccacca ggaactcagc gtgccgtcaa agttgatgta agtctggatc
      181 agtccctgaa ttgccacgcc aaaaagcgtc ccaagcaccg tcccgacgcc accacttaac
      241 aacgtaccgc caatcaccac cgacgcgatg gcgtccagtt ccacacctac gcccgccagt
      301 gcatatccgg cttgggtata aatcgagaag acaatccccg ccagtgttgc cagtccggtg
      361 gagagcatat aaatgcgaat agtggtgctg cgagtagaaa tccccatcag gttcgccgac
      421 gtcgcgttgc cgccaatagc gtacacctga ttgccaaaac gggtacgatg cgcaaggaaa
      481 ataccgataa ccaccaccgc cagcatcaac agccccatcg cactaaggcg accgccgccg
      541 gggattttcc acgcaaggct tgagagcgtg tcataaatgg gatggtttat cggaatcgac
      601 tcttccgaaa cgagatagct gacgccgcgc aaaaagaaca tccccgcaag cgtaatgata
      661 aatgccggga tcttcagggc gtcgatcaga agccccataa atgcgccgaa ggcacagccc
      721 atcaccagca ctagcggaaa cgccagcagc ggcgagaggc cgaaatcgcc aaccactttt
      781 gccagaaaca cgccagtaaa ggcgatcacc gaaccgacgg agagatcgat accgccagag
      841 aggatcacaa aggtcatgcc aacggcgatt atcccaagaa aggcgttatc ggtcaggata
      901 ttgcagatca cccttgtgga agcaa
//
LOCUS       DAJTYI010000294          910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYI010000294 DAJTYI010000000
VERSION     DAJTYI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..602
                     /locus_tag="OO564_004095"
     CDS             <1..602
                     /locus_tag="OO564_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6654971.1"
                     /translation="NTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNG
                     WENNVKSIDALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTP
                     RAQYPTAPGYATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRAS
                     SQDKRYHIVEGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            602..>910
                     /locus_tag="OO564_004096"
     CDS             602..>910
                     /locus_tag="OO564_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6654972.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     Y"
BASE COUNT          247 a          208 c          234 g          221 t
ORIGIN      
        1 acaatacccg aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg
       61 caattcagga tcgccgcatc aaggccatcg gtaccgttag tgcggtcaat attggttcga
      121 tgttccgcaa tggttgggaa aacaatgtga aatctattga tgctctgcca cacattgaag
      181 ctggctcaaa cgcccgaacc agcgatatta gcagcgaaga atacgccatt atgccactgg
      241 ctccaatgaa ggaggccgat gcgccgaacg aagaactgcg tcaggcctgg gagtattacc
      301 acacacctcg tgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc
      361 agattattac ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatgcaga
      421 ttgtggcggg cagccaggca gggagcaaat ggatgagtga cgacctgtac gatcgggcat
      481 caagccagga taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgacg
      541 gcaaagccta tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt
      601 aatgaaacat attcaaatcc gcaatagtga tatggcctgg catattgctg ccaatattca
      661 gtttccacca aattttgatg agtcaaaaca gtatccggcg attatcagcg tccatccttt
      721 tggcagttgt aaggagcaga cgtctggcaa tatctatggc aaggcattgg ctgaaaaagg
      781 ctatgtcgtt ctcgcttatg acgccagttt ccagggagaa tctggtgggg agcctcgttg
      841 gatagaagat ccgacacagc gcgtagagga tattagccgt gttatcgatt atgcagtaac
      901 tctgccatat
//
LOCUS       DAJTYI010000295          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYI010000295 DAJTYI010000000
VERSION     DAJTYI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..236
                     /locus_tag="OO564_004097"
     CDS             <1..236
                     /locus_tag="OO564_004097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654973.1"
                     /translation="FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTE
                     REFATILAGISAGGMYCPECGTVHWPDGVAPPF"
     gene            236..>909
                     /locus_tag="OO564_004098"
     CDS             236..>909
                     /locus_tag="OO564_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6654974.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFL"
BASE COUNT          195 a          231 c          275 g          208 t
ORIGIN      
        1 gttttgacga tgccgttgaa tgctgcgcag acctgaatat tccgttcttt gtggatgcgg
       61 gaaacaaaaa gctggtcttc tggtttgtac gtgttgatga cgaagggtat ccggaaatcg
      121 cccgctgcac ggagcgggaa tttgcgacca ttcttgccgg tatcagcgcc ggcggtatgt
      181 actgcccgga gtgtggcacg gttcactggc cggacggagt cgccccgccc ttctgatgct
      241 tccccgtttt gccgatattt ttcagcaggg aaaccgctgg cttaactggc tggagaaaca
      301 accggaaggg gcagtccgtc cggtggtaat tgaatcagtg acaaaaatca tggcctgcgg
      361 gaccacactg atgggctaca cgcagtggtg ttgttcatca ccggactgtt gccatacaaa
      421 aaaggtctgc ttccggtgta aaagccgctc ctgcccgcac tgcggggtga aggctggcgc
      481 acagtggata cagtatctgc tgagcctggt ccccgactgc ccgtggcagc atattgtgtt
      541 cacacttccc tgccagtact ggtccctggt gttccacaac cggtggttac tggcagagat
      601 gagccgcatt gcagcggatg tgatactgga aatctgccat caggcagatg tggagccggg
      661 gatattcacg gtgatccaca catgggggcg tgaccagcag tggcatccgc atatccattt
      721 atcgacaact gccggtggtg tgacgtcggg ccacacctgg aaaaatcttc atttttacgc
      781 ccgtaaggtg atgagcatgt ggcgctaccg gataacacgg ttgctgtcac ggaaataccc
      841 tgacctggtg atgccggatg cgctggcagc agaaggaagc agtaaacggg aatggaatcg
      901 cttcctgga
//
LOCUS       DAJTYI010000296          854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYI010000296 DAJTYI010000000
VERSION     DAJTYI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..192)
                     /locus_tag="OO564_004099"
     CDS             complement(1..192)
                     /locus_tag="OO564_004099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654975.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            239..854
                     /locus_tag="OO564_004100"
                     /pseudo
     CDS             239..854
                     /locus_tag="OO564_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          215 a          239 c          258 g          142 t
ORIGIN      
        1 ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg cggcaaccaa
       61 tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt cggcagcgtc
      121 atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt cagcagcgaa
      181 gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg cgacggcggc
      241 agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag cagcaaaacg
      301 ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga aaaaggggat
      361 agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga cagcgaaagc
      421 ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc cggctctgac
      481 cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga tcgccagtac
      541 ggcttatgtt atggccgcga ttgctgccct cgtggactcg tcgcctgatg cactgaacac
      601 gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca ccatgactaa
      661 cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg ggcttgctac
      721 tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg caaccctgac
      781 aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg aatacctcgg
      841 tttacaggaa acgg
//
LOCUS       DAJTYI010000297          853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYI010000297 DAJTYI010000000
VERSION     DAJTYI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>853
                     /locus_tag="OO564_004101"
     CDS             <1..>853
                     /locus_tag="OO564_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR6654976.1"
                     /translation="RTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQ
                     KRRILPALSVIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLT
                     WLRQAPLKPNSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLG
                     KFEPQRRYATLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAIND
                     KVRLYSRIGQALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVG
                     ENFATLRR"
BASE COUNT          168 a          285 c          274 g          126 t
ORIGIN      
        1 gcgcacctac ctgcaactgg ccccgttcgg cctgtccgac ttccgcgccc tggtgcgcga
       61 gctaaccgag ctggcccagc agaccgacaa aggcttgctg ctggccggtc aggccctgga
      121 gagcctacgg cagaaacgac gcatcctgcc ggcgctgagc gtgattgacc gggcctgctc
      181 ggaagccatt gcgcgagcca atcggcgggt ctaccgcgcc ctggtcgaac cactcacgga
      241 ctcgcatcgg gccaagctgg acgagctgtt gaagctcaag gccggcagca gcatcacctg
      301 gttgacctgg ctgcgccagg caccgctgaa acccaactct cggcacatgc tggaacacat
      361 cgagcggctg aagacatttc agttggtgga cttgcccgaa ggcctgggcc ggcacatcca
      421 ccagaaccgc ctgctcaagc tggcccgcga gggtgggcag atgacgccca aagacctcgg
      481 taagttcgag ccgcagcgcc gctacgcgac cctggccgcc gtggtgctgg agagcaccgc
      541 gaccgtgatc gatgagctgg tcgatctgca tgaccgcatc ctggtcaagc tgttcagcgg
      601 cgcgaagcac aagcatcagc agcagttcca gaagcagggc aaggcgatca acgacaaggt
      661 gcgcctgtac tccaggatcg gccaggcgct gctggaagcg aaggaaagcg gcagcgaccc
      721 ctatgccgcc atcgaggcgg tgattccctg ggacgagttc accgagagcg tcagcgaggc
      781 cgagctgctg gcccggccgg aaggcttcga ccacctgcac ctggtcggcg agaacttcgc
      841 caccctgcgc cgt
//
LOCUS       DAJTYI010000298          843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYI010000298 DAJTYI010000000
VERSION     DAJTYI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..764
                     /locus_tag="OO564_004102"
                     /pseudo
     CDS             1..764
                     /locus_tag="OO564_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          227 a          182 c          200 g          234 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agc
//
LOCUS       DAJTYI010000299          832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYI010000299 DAJTYI010000000
VERSION     DAJTYI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            134..625
                     /locus_tag="OO564_004103"
     CDS             134..625
                     /locus_tag="OO564_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654977.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT          221 a          229 c          195 g          187 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcgctaaaga
      421 gctagcgtct cagaaagagc gtacccgcga aatgttcatg accgaagacg aatggcaaaa
      481 agagctacag cgccgcgaac aggtcaaagc tgaacgccgg acctatggtg aaaacctccg
      541 cagcgctact cattccgctg gtcgctcacg tgccgctatc gtcgctgacc ttgagtcagg
      601 cggtaactgg atggacagct tgtaagcaag cgtccctcat ctgagaacac acggtccctg
      661 actgcctgtt agggacttca agtatttgac tcaaggagat taattataat gaatatgact
      721 aaaaacgctt tccagtttgg cattgagcct gttcatatca ccgacaacga caaccttcag
      781 gttaatgagg gccttccgac taacgccgac cctcaggtct atgccctcca gt
//
LOCUS       DAJTYI010000300          826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYI010000300 DAJTYI010000000
VERSION     DAJTYI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..820
                     /locus_tag="OO564_004104"
     CDS             <1..820
                     /locus_tag="OO564_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6654978.1"
                     /translation="IARRYRTYFGKGADRESLRVLNRDLRSAFNALQQDKPLNDRQKA
                     AMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRHYSPTEFRKKRLAEEDTEIEW
                     LSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYDIHDAERITVR
                     RKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAEDEGIQILEHK
                     QAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRLGMQKLFAMQEEDDPLA"
BASE COUNT          236 a          184 c          235 g          171 t
ORIGIN      
        1 tatagccagg aggtatcgca cctattttgg caagggtgct gaccgggaat cgttgcgtgt
       61 acttaaccgt gatctgcgtt cagcgtttaa cgccctgcaa caggacaagc ctctgaacga
      121 ccgccagaaa gcggcaatgc gcgagctgcc atcgtgggcg gagcttattg aggctattcg
      181 tgaaggcgtg gaatggtaca acaaccgccc gcattctgag ctgccgatga aaccgaacgg
      241 acggcattac agcccgacag agttcagaaa aaaacgcctg gcagaagaag acaccgaaat
      301 tgagtggctg agtgatcttg agctgcgcga catgttccgc ccaatggtgg aacgaccggt
      361 aagacgctgc gaaattcagt ggctgaacaa tatttactac gcgccggagt tgcgtgacga
      421 acacggtcgc aaagtgctta tcagttatga cattcatgac gccgagcgaa ttacggtacg
      481 tcgcaaggac ggcagcttta tctgtgaggc gatatggaac ggcaataaac gcgcagcctt
      541 tgctgtcagt gctgaatatc acaaacaaca gcagcgtatt aaaggaatgc gcaaacgcgc
      601 tgaggaaaaa attcgtgatg ctgaagatga aggtattcag attctggaac acaaacaggc
      661 tgaaccctgg ctgagtaatg tttatcgccc ggttggtaat gtggtggcag ttcagcaacc
      721 ggaatatgaa gaagagcatg acgaagaatt tgaacgcgat ttccggctgg ggatgcaaaa
      781 actttttgcc atgcaggaag aagatgatcc gctggcctga ataaaa
//
LOCUS       DAJTYI010000301          826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYI010000301 DAJTYI010000000
VERSION     DAJTYI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..804
                     /locus_tag="OO564_004105"
     CDS             <1..804
                     /locus_tag="OO564_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6654979.1"
                     /translation="LADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDM
                     SEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQ
                     VLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFR
                     PEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGY
                     DPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAVQ"
BASE COUNT          190 a          182 c          237 g          217 t
ORIGIN      
        1 ttggcggatc caaatcgccc gattggttca ttcctgttcc tcggcccaac gggtgtgggg
       61 aaaactgagc tttgtaaggc gctggcgaac tttatgtttg atagcgacga ggcgatggtc
      121 cgtatcgata tgtccgaatt tatggagaaa cactcggtgt ctcgtctggt tggtgcgcct
      181 ccgggatatg tcggttatga agaaggtggc tacctgaccg aagctgtgcg tcgtcgtccg
      241 tattccgtca tcctgctgga tgaagtggaa aaagcgcatc cggatgtctt caatattctg
      301 ttgcaggtac tggatgatgg gcgtctgact gacgggcagg ggagaacggt cgactttcgt
      361 aatacggttg tcattatgac ctctaacctc ggttccgatc tgattcagga acgcttcggt
      421 gaactggatt atgcgcacat gaaagagctg gtgctcggtg tggttagcca taacttccgt
      481 ccggaattca ttaaccgtat tgatgaagtg gtggtcttcc atccgctggg tgaacagcac
      541 attgcctcga ttgcgcagat tcagttgaaa cgtctgtaca aacgtctgga agaacgtggt
      601 tatgaaatcc acatttctga cgaggcgctg aaactgctga gcgagaacgg ttacgatcca
      661 gtctatggtg cacgtccttt gaaacgtgct atccagcagc agatcgaaaa cccgctggca
      721 cagcaaatac tgtctggtga attggttccg ggtaaagtga ttcgcctgga aattaatgaa
      781 gaccggattg tcgctgtcca gtaatgataa aacgagccct tcgggg
//
LOCUS       DAJTYI010000302          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYI010000302 DAJTYI010000000
VERSION     DAJTYI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..711
                     /gene="curA"
                     /locus_tag="OO564_004106"
     CDS             <1..711
                     /gene="curA"
                     /locus_tag="OO564_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6654980.1"
                     /translation="GDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETL
                     VVAAATGPVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQL
                     AKACPKGIDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLM
                     ATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIG
                     LLKGKNFGKVVIRVAGDD"
BASE COUNT          210 a          171 c          242 g          200 t
ORIGIN      
        1 ggtgatgacc tggtgaaact tggcgatcat ccgcaaaatc catcgtggtc gctgggtgtg
       61 ctggggatgc caggctttac cgcttatatg gggctgctgg atatcggtca gcctaaagag
      121 ggcgaaacgt tggtggtagc tgcggcgaca ggaccagtgg gggcgacggt ggggcaaatc
      181 ggcaaactta aaggttgcag agtgatgggg gtagccggtg gcgcggaaaa atgccgccat
      241 gctaccgagg tgttaggctt cgatgtttgc cttgattacc tcgcggacga ttttgccgaa
      301 caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt gggcggtaag
      361 gtatttgatg cagtgctacc attacttaat acatctgcgc gcattcccgt ttgtggctta
      421 gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc cctgttgatg
      481 gcaacggtgc tgaaaaaacg tattcgcttg caagggttta ttatcgctca ggattatggt
      541 caccgcatcc atgagtttca gaaggagatg gggcaatggg tgaaagagga taaaatccac
      601 taccgcgaag atattactga tggtttagag aatgcgccac agacgtttat cggcctgctg
      661 aagggtaaaa acttcggcaa agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac
      721 ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt taattaacta
      781 accacgtcat tgttctgctg ctaataacaa caaaggtggg tgg
//
LOCUS       DAJTYI010000303          822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYI010000303 DAJTYI010000000
VERSION     DAJTYI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..789)
                     /locus_tag="OO564_004107"
     CDS             complement(<1..789)
                     /locus_tag="OO564_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6654981.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEK"
BASE COUNT          187 a          235 c          192 g          208 t
ORIGIN      
        1 tttttcgctc atattcgttt caaccattgc gccgtgaata tcaaaaaagc gcgagcaaat
       61 atcgccagca acctggcggg cattcaggtc gtcactctct tcaccaccat aaaacgcatg
      121 ccagttagcg ccgtcgcgaa tggccggtga gccaattccc accagggcga catccagatt
      181 atcccagtag gcagagatgg ttttaaagtg ctgagactgc atgatcccat tacgaattaa
      241 tgggttatcc agcagagccg gaaaatccgc gagatgcgat tcccctttca gcttcgctgc
      301 cgcgctgtag gttaatgtgt ttacatgata gcggctttcg agtttaccgg acggtccgcc
      361 aataattggt acgcagatta actgccgcga ttgccccgcc tgcggcaagt tttcaaccag
      421 tgcgctcacc gcgcgacccc aggaaaaacc gacaatatcg ccaggctcca gcaagcgatc
      481 cagcagttgc gcgccgtgta accccatcat cgccagttga gtctcttcat cctcatcatt
      541 tcccgacacc accacaacgt ctttcaggcc aaacttttgc ttcagttgct gctccagcca
      601 gagattttcg ttgtagtcat agttgatggc gatggtgacc attccctgat cgcggcctcg
      661 tttaagcaag cggctgatgg tggtgcggta aatacccagt tcgcgcgcga tttgcgcctg
      721 cgtcatatcc tgttcgtaat agagttgggc aatcttcaca atcaaacgga tatcgtcact
      781 gttttccatt gccgcctcct ggttctgcac atttgtgcaa at
//
LOCUS       DAJTYI010000304          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYI010000304 DAJTYI010000000
VERSION     DAJTYI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..254)
                     /gene="ospG"
                     /locus_tag="OO564_004108"
     CDS             complement(<1..254)
                     /gene="ospG"
                     /locus_tag="OO564_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6654982.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEAS"
     gene            complement(283..509)
                     /locus_tag="OO564_004109"
                     /pseudo
     CDS             complement(283..509)
                     /locus_tag="OO564_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(506..775)
                     /locus_tag="OO564_004110"
     CDS             complement(506..775)
                     /locus_tag="OO564_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654983.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
BASE COUNT          207 a          210 c          156 g          226 t
ORIGIN      
        1 gatgcttcat cgccataaaa agcattaaaa agctcagatt cttgccaagc catttccaga
       61 atctcattgt actggttgcc aataagatca tactttttat acaaagcaga tgaatcattc
      121 acatcttcaa agatttctgc tgtcgacccc tgacctatta acttaccgag aatgggctcc
      181 gttactatgc cagcatgaga attattattc tcaaatggaa aaggtgtttg aataatggta
      241 gatgttattt tcatgtcata cctttgttac gatatttaaa aatcacaaga gattttcaca
      301 caaaataacg ttacacgcta ccttgtgtgg tccagatgtt ccttccacac gaccgttgcg
      361 gcctttatac ggctgccctg ccaggctgct ggctgaataa ccaatccgtt cgtccggttc
      421 aatggcggcc atcaggttct gctccgcaat gtactacgaa tgcgcttcct gtcctggatg
      481 gccagcatgg tccagaacat ctccatcatt tatcagcaat cttcctgctc atttcatccc
      541 gccatgcagc agcagaggat tcgaccatgt catggggaag cacatcaccg cattcgagtt
      601 gctgacggcc tgccgcaacc tggtcacgga accaagaatt gtgctgccgt tcaacggtct
      661 ggcgcataaa atcccggatt aactgagagc cattacggtc catgcttttt gcagcggcca
      721 taaaagcatc cttcagttct gcgtcgattc ttacactcat attaacctgt gccatttgcc
      781 acctcgtcat taccacgta
//
LOCUS       DAJTYI010000305          791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYI010000305 DAJTYI010000000
VERSION     DAJTYI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            56..>791
                     /gene="mppA"
                     /locus_tag="OO564_004111"
     CDS             56..>791
                     /gene="mppA"
                     /locus_tag="OO564_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6654984.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFL"
BASE COUNT          212 a          179 c          210 g          190 t
ORIGIN      
        1 ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga aacttatgaa
       61 gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc tttcgtatgc
      121 tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc gccacattaa
      181 agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga ttcaggtcat
      241 tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg tccccggcgt
      301 cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc gcgataacgc
      361 aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct ggcaacgtct
      421 ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa
      481 cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca ccgcagttga
      541 tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga atttaaccgc
      601 taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag agtggacgaa
      661 acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa
      721 actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc tgcaaaaagt
      781 gaccttcctg c
//
LOCUS       DAJTYI010000306          782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYI010000306 DAJTYI010000000
VERSION     DAJTYI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..70)
                     /locus_tag="OO564_004112"
     CDS             complement(<1..70)
                     /locus_tag="OO564_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor B"
                     /protein_id="HCR6654985.1"
                     /translation="MANPEQLEEQREETRLIIEELLE"
     gene            232..>782
                     /locus_tag="OO564_004113"
     CDS             232..>782
                     /locus_tag="OO564_004113"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HCR6654986.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLD"
BASE COUNT          202 a          192 c          178 g          210 t
ORIGIN      
        1 cttccagtaa ttcttcaata atcaaacgtg tttcttcacg ctgttcttcc agttgttccg
       61 ggtttgccat ggctcattcc tcttaatgtc ctgccgatac tcttattgtc acacaccact
      121 gacattgcct ccacctttgt gacaaagatt tatgctttag acttgcaaat gaataatcat
      181 acacataaat ttaattttaa ttcattgagg cgttagccac aggagggatc tatgtccggg
      241 ttttatcata agcatttcct gaaattactc gatttcacgc cagctgaact caacagcctg
      301 ctgcagttag ccgcgaagct gaaagctgat aagaaaagcg gtaaagaaga agccaaactc
      361 actggtaaaa acatcgcgct catcttcgaa aaagactcga ctcgtacccg atgctctttc
      421 gaagttgccg catatgacca gggtgctcgc gttacttatc ttggcccaag cggtagtcag
      481 attggtcata aagagtcgat taaagacact gcccgcgtgc ttggtcgcat gtatgacggt
      541 attcagtatc gcggctatgg tcaggagatt gtcgaaacac tggcggaata cgctggcgtg
      601 ccggtatgga acggcctgac caatgagttt caccccacgc agttgctggc ggatctcctc
      661 accatgcagg agcatttacc cggtaaagca ttcaacgaaa tgacgctggt ctatgcaggt
      721 gacgcacgta acaacatggg caattcgatg ctcgaagctg cggcgcttac cggtctggat
      781 tt
//
LOCUS       DAJTYI010000307          781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYI010000307 DAJTYI010000000
VERSION     DAJTYI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..624)
                     /gene="ospI"
                     /locus_tag="OO564_004114"
     CDS             complement(<1..624)
                     /gene="ospI"
                     /locus_tag="OO564_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6654987.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKS"
BASE COUNT          242 a          187 c           97 g          255 t
ORIGIN      
        1 acttttccaa ctactactgt tagcatagtg atcaaattcc tgatcagata catgaattac
       61 ctgaaccgaa ctattttcat ctacaaatgc atctggcgat ttttctacag gatcataaag
      121 attaaatccc tttcctgttt gaacatccaa aaacataatt ttttcaccat cgttaaatcc
      181 agctatccaa tgagtggcct gttcaataga aatcattact acacttcctt ttgaagtatt
      241 ttgtaaaata ttttctgcaa tacgctcacg aaatttacta taagtttcat taggatgtga
      301 cactatgcct tcactatcaa tttgctctaa atgcctatga aaaaatccgg tcatatactc
      361 tgatatttca ttaggtacgg gagcctctct tactccatac cctaacatag ccatacaagc
      421 atgaagcgca caccctgaac agttaccatc cccaccagac ggatttatca ttttctgtaa
      481 agtcgctgcc aattcagaat ctttatccat atcaccaaga cttttagagt ttaaagattc
      541 cagcaatttt ttaacattta catttccctc ctctgtatgt gcctcatatc cagcagttcc
      601 ctgtaacgac accccattaa tcatattgta tcctgacagt atatatactc actatatttt
      661 cttcataaca aatatccctc tgatatgaca acaaaaaacg gagccggatt cctgtttttg
      721 tgaactgcat ctattcgtgt ctaaaaagtg atccacttac caccaacggc ctaatatata
      781 c
//
LOCUS       DAJTYI010000308          781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYI010000308 DAJTYI010000000
VERSION     DAJTYI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(135..471)
                     /locus_tag="OO564_004115"
                     /pseudo
     CDS             complement(135..471)
                     /locus_tag="OO564_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          250 a          148 c          136 g          247 t
ORIGIN      
        1 cttcagcaca ctgctaaaac gcatagtgat cgagcgctga ttctggcgaa caactgaact
       61 aatacatcag aatctgcatt atgttaaata aatatgaaaa gatggtttaa ataccccgtt
      121 acttttgact tacactatac ggtatcgcat cgtttaatat tcgcaccggc cagattttta
      181 tttctattag ttgtcacaat actgaatgcg tacgaccaca gtattctggc tcctgtgtgg
      241 ttatgcttta attctgcgtt ccgggcagat aagcagttgc ttgcaggaat ccttcttgtg
      301 ttaatgtcag ttcccctttt taccagtgct gatttccaca ttccgtccaa cagagcttat
      361 agcctttccc tggattatag cattgtccgg ctgaagttct ttttgaataa taatagaagc
      421 actgctggca gatccagtcc gtttttcata acccactgta ctgataacca taatctaatc
      481 agtagaaatt gagtcgaaaa taagcactac tccatacagg ataattagag gtcagtttga
      541 ttattcacaa ttcatcatca gcattttcta tttctgacga aatcaacatg aaaataacca
      601 tatatgataa ttattataat aacggcttta attggaatac atatattaca acgtattata
      661 tataattggt attctgggaa ctatattctc aaaatacagt agaaacgagg tatgtttctg
      721 gtggaaagga cagtgggatt aaaaagtaag aattgataaa aaaaacgcca gcaacacata
      781 g
//
LOCUS       DAJTYI010000309          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYI010000309 DAJTYI010000000
VERSION     DAJTYI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..721
                     /gene="rlmB"
                     /locus_tag="OO564_004116"
     CDS             <1..721
                     /gene="rlmB"
                     /locus_tag="OO564_004116"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6654988.1"
                     /translation="IYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALESQGV
                     VIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDP
                     HNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRML
                     QEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSV
                     SSLNVSVATGICLFEAVRQRS"
BASE COUNT          171 a          200 c          221 g          171 t
ORIGIN      
        1 gatttacggc atccacgcag tgcaggccct gctggagcgc gcccctgaac gttttcagga
       61 agtctttatt ttaaaaggcc gtgaagataa acgtctgtta ccgctgattc acgcccttga
      121 gtctcagggc gtggttatcc agttggcaaa ccgccaatat ctcgacgaga aaagcgacgg
      181 tgccgtgcat cagggcatta tcgcccgcgt gaagccagga cgtcagtatc aggaaaacga
      241 tctgccggat ctgatcgctt cgctcgatca accgttcctg ctgatcctcg acggcgtaac
      301 cgatccgcac aacctcggcg cgtgcctgcg tagtgcggac gccgcaggcg ttcatgcggt
      361 gattgtgccg aaagatcgct ccgcacagct caacgctacg gcgaaaaaag tagcctgcgg
      421 cgcggcagaa agcgttccgc tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt
      481 gcaggaagag aatatctgga tcgtcggtac ggcaggcgag gcggatcata cactctatca
      541 gagcaaaatg accggacgcc tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg
      601 cctgacccgt gaacattgcg atgagttgat cagcatccca atggcaggaa gtgtttcttc
      661 cctgaacgtt tcggttgcaa ccggaatttg cttatttgaa gcggttcgtc agcgcagcta
      721 gttcctctga attatgaaaa gccatccaga tttggatggt ttt
//
LOCUS       DAJTYI010000310          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYI010000310 DAJTYI010000000
VERSION     DAJTYI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>747
                     /gene="btsS"
                     /locus_tag="OO564_004117"
     CDS             <1..>747
                     /gene="btsS"
                     /locus_tag="OO564_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6654989.1"
                     /translation="VGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIR
                     RGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAA
                     LFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIG
                     AVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQ
                     CKLGSTLVIPLRGENQRVMGTIKLYEAKNR"
BASE COUNT          171 a          181 c          207 g          188 t
ORIGIN      
        1 tcgtcggtgg gctggttggt ctgaccggcg gcttacatcg atattcgatg gggggcatga
       61 ccgcgctaag ttgcatgatc tcaaccatcg ttgaaggatt gctcggcggc ctggtacaca
      121 gcatcctgat ccgtcgcggg cgcactgata aagtctttaa ccccattacc gccggtgccg
      181 tcacgttcgt cgctgaaatg gtgcaaatgc tgatcatcct tgcgatcgcc cgaccttatg
      241 aagatgcggt gcgtctggtg agtaatattg ctgcgccaat gatggtcacc aataccgtcg
      301 gcgcggcgct gtttatgcgt atattgctcg ataaacgcgc gatgtttgaa aaatacactt
      361 cggctttttc tgccactgcg ctgaaagtgg cagcctcgac ggaaggcatt ttgcgacagg
      421 ggtttaacga agtgaacagc atgaaagtgg ctcaggtgct gtatcaggag ctggatattg
      481 gtgcagtcgc gattaccgat cgagagaaat tgctggcctt taccggaatt ggtgacgacc
      541 accatttacc cggcaaaccg atttcttcga cttacacctt aaaagcgatt gaaaccggtg
      601 aagtggtcta cgctgatggc aacgaagtac cttatcgttg ctctttgcat ccgcaatgca
      661 aactggggtc gactctggta attccgttgc gtggcgaaaa tcagcgcgtg atgggcacca
      721 tcaaattgta tgaagccaaa aaccgtt
//
LOCUS       DAJTYI010000311          744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYI010000311 DAJTYI010000000
VERSION     DAJTYI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            62..358
                     /locus_tag="OO564_004118"
     CDS             62..358
                     /locus_tag="OO564_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6654990.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            364..>744
                     /locus_tag="OO564_004119"
     CDS             364..>744
                     /locus_tag="OO564_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6654991.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGL"
BASE COUNT          215 a          172 c          187 g          170 t
ORIGIN      
        1 gcaatccctg acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt
       61 gatgagcgct tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca
      121 gcagatcgtt cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc
      181 tcagcacctg tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca
      241 ggcaatcagc cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat
      301 ccggtacatg gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa
      361 tctatggcgc tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg
      421 gggtttgctg gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg
      481 cttatgcggc aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa
      541 acaggctctg actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca
      601 aaaacccgct cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc
      661 gtaatgatca tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga
      721 cgccgaaacc gtaaacgagg actg
//
LOCUS       DAJTYI010000312          734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYI010000312 DAJTYI010000000
VERSION     DAJTYI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>734
                     /locus_tag="OO564_004120"
     CDS             <1..>734
                     /locus_tag="OO564_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6654992.1"
                     /translation="CGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLY
                     LLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAIS
                     SIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSA
                     EALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFL
                     LHINVSPWQLNEPHFHERFTTIMKEN"
BASE COUNT          198 a          149 c          180 g          207 t
ORIGIN      
        1 tgtgtggtct ggaagccgtg gaagcggtgt ttggtgttga tgaacgcaat aaaatcgtca
       61 ggaaattgtg tcagcgaatt gccgagaaat atgcgcaatg ctgcgatatc gtgacattca
      121 atgccgatct ctatttactt ctgtgtcggg aaaatgtaca gacatttacc cgtaaaatag
      181 cgatggtaaa cgattttgac agcagctttg gctaccgcaa tctgcgcatc cataagtctg
      241 ccatttgtga acctttgcag ggggaaaacg cctggagtta cgcagaaaaa ctgaaactgg
      301 cgatttccag tatccgtgac catatgttct ccgagtttat tttctgtgat gacgcgaaac
      361 tcaacgaaat agaagagaat atctggattg cgcgtaatat tcgccatgca atggaaattg
      421 gcgaactatt cctcgtctat caaccgatcg ttgatattaa cacccgcgcc attctgagcg
      481 cggaggcgtt gtgccgttgg gtttctgcgg agcgggggat catttcaccg ctgaagttca
      541 ttaccattgc tgaagatatc gggtttatca atgagctggg ttatcagatt attaaaacgg
      601 caatgggtga attcagacat tttagtcaac gtgcgtcgct gaaggatgat ttcttactgc
      661 atattaatgt ttcgccctgg cagttaaacg aaccgcactt tcatgagcgt tttaccacca
      721 tcatgaaaga aaat
//
LOCUS       DAJTYI010000313          730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYI010000313 DAJTYI010000000
VERSION     DAJTYI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(177..713)
                     /locus_tag="OO564_004121"
     CDS             complement(177..713)
                     /locus_tag="OO564_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6654993.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREMIEGKQKSWLTHCTDAEAL
                     KIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWL
                     RVAEFILYSPMREAFDYA"
BASE COUNT          171 a          204 c          137 g          218 t
ORIGIN      
        1 ctgcaaactc atctgaccac cagcacgttt aacgtcctgt gccatttttc aggcataaaa
       61 aaacccgctc aatggcgggt tctggtaaag ttcatgcgct tggttcgcct cgcgatacag
      121 ctttgcgaag ctgtacctga attgaagcag tttgtggctc attttgcaat gattttttaa
      181 gcataatcga acgcttctct catcggtgaa tacaaaatga actcagcaac acgcaaccac
      241 tgatcaacac gacgtcggca tgtaatcaac gcccactctg ggtactgttc gtttaataac
      301 tcggccatcc ttctcttact catccctcgc cccacataac gctgactcag gacatttagt
      361 agtcctggat gatccgccag aacttcacct ataacactat caatttttag tgcctctgcg
      421 tcagtgcagt gagttaacca gcttttttgc tttccttcga tcatctcacg caaaaatgct
      481 tccaactctg gtttatcaat tcccgctttt ttcaatctgc gcagggcttc attgacggct
      541 gtttttgtca ttttttggga tgccagcaac tggttaaaca tattccctga cctgccgccg
      601 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      661 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cacaaatatc
      721 cctcctttct
//
LOCUS       DAJTYI010000314          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYI010000314 DAJTYI010000000
VERSION     DAJTYI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..536)
                     /locus_tag="OO564_004122"
     CDS             complement(<1..536)
                     /locus_tag="OO564_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6654994.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGR"
BASE COUNT          175 a          169 c          170 g          188 t
ORIGIN      
        1 cgaccggtga atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa
       61 tgtacctgct ggatgcactg gcgtgtcaaa gggccaacca tactttcgct ttttttctgg
      121 tacacgccac cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag
      181 ctgctgaccg tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg
      241 gcattgctgc tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca
      301 aactcggcga gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca
      361 ctatcaagcg aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg
      421 ttgagatcct ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg
      481 ctaacctgac cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct
      541 gtaattctta taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa
      601 ataggaaaac ctgaaggcac gattgttaaa tctcccacgg tgtcttcggg atgtctggcg
      661 tagattcagc aacctggcgc aagagttctg ctttaagtat ct
//
LOCUS       DAJTYI010000315          699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYI010000315 DAJTYI010000000
VERSION     DAJTYI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..293)
                     /locus_tag="OO564_004123"
     CDS             complement(<1..293)
                     /locus_tag="OO564_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR6654995.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVV"
     gene            complement(290..>699)
                     /locus_tag="OO564_004124"
     CDS             complement(290..>699)
                     /locus_tag="OO564_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6654996.1"
                     /translation="STETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYG
                     AQKASQAVETLFSQLAARLAAEGMTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVP
                     WVNALDKPVSLALKSGNFGDEAFFSRAQREFLS"
BASE COUNT          166 a          190 c          182 g          161 t
ORIGIN      
        1 tgcaccaccg ctttacagcg cggattgttg cgatacagcg ccagatgaaa gagcacctct
       61 ttcgagggtt tgtcaccact taaccattcg ccatccgcgg cgactttgga aagccgctgc
      121 ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca gtaaattccc gtcaggtaaa
      181 agcagcgaca gattgccagc cgaaccggtt gcatagccgc gctgaaagaa tgaactggca
      241 atccgcgtca tctcctctcg caaagactgc tctacttttg cgaaatcgct catgataaaa
      301 actctctttg ggctcgtgaa aaaaaggctt catcaccgaa gttgccagat ttaagggcga
      361 gtgagacagg cttatccagt gcgtttaccc acggcacgcc gggggaaatg gttgggccaa
      421 tatgaaaccc ttttatgccc aggctctgtg tgactacgcc ggaggtctca ccacctgcga
      481 caataaagcg tgtcatgcct tccgctgcta accgcgccgc tagttgagaa aacagagttt
      541 ctactgcctg actggctttt tgtgcaccgt attgctgttg aattgctgcc aatgcgtcag
      601 tgctggcggt ggcaaaaacc agtggagcaa gtacactttc ctggcccaga acccactctg
      661 ccagttcgtg agcataagcg gccagagttt cggttgaga
//
LOCUS       DAJTYI010000316          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYI010000316 DAJTYI010000000
VERSION     DAJTYI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            61..336
                     /locus_tag="OO564_004125"
     CDS             61..336
                     /locus_tag="OO564_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654997.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            355..>695
                     /gene="mdtN"
                     /locus_tag="OO564_004126"
     CDS             355..>695
                     /gene="mdtN"
                     /locus_tag="OO564_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6654998.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQ"
BASE COUNT          155 a          185 c          166 g          189 t
ORIGIN      
        1 cttcaatgga tattgatatt cactacagat attgttcgca gcagacagga tgtatttaat
       61 atgccaacag ttctctctcg catggcgata cacctcaaaa aaacagcctg gataattccc
      121 gtcttcatgg tttcgggatg ctcattatct ccggcaatcc cggtgatcgg cgcttattat
      181 cccagctggt ttttctgcgc cattgccagc cttattttga cgctcatcac gaggcgagtt
      241 attcagcggg caaatatcaa tctggcattt gtcggaatta tttataccgc cctttttgct
      301 ctctacgcca tgctgttctg gctggcattt ttctaatcta tttgagacgc gctcatggaa
      361 agtacgccga aaaaagcacc tcgcagtaaa ttccctgctc tgttagtggt tgcgttggcg
      421 ctggttgccc ttgttttcgt tatctggcgc gtagacagtg cgccatcaac taatgacgct
      481 tacgcgtcag cagataccat tgatgtggtg ccggaagtca gcggtcgcat tgtagaactg
      541 gcggtcaccg acaaccaggc ggtgaaacag ggcgatttgc tgttccgcat cgacccgcgc
      601 ccgtacgaag ccaacctggc gaaagccgaa gcctccctcg cggcgctgga taagcaaatt
      661 atgctcaccc agcgtagcgt tgacgcccaa cagtt
//
LOCUS       DAJTYI010000317          687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYI010000317 DAJTYI010000000
VERSION     DAJTYI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..314)
                     /locus_tag="OO564_004127"
     CDS             complement(<1..314)
                     /locus_tag="OO564_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6654999.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQP"
     gene            complement(450..>687)
                     /locus_tag="OO564_004128"
     CDS             complement(450..>687)
                     /locus_tag="OO564_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HCR6655000.1"
                     /translation="LKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLA
                     ARQVLSRSETVLQKSLEITEEADLALRNAMARIK"
BASE COUNT          171 a          170 c          145 g          201 t
ORIGIN      
        1 ggttgttcag cccgggcgtt tgggtcgcag ctgagcgctt gtttcacgct cgctgcttcc
       61 acgttaaagc cagtaaacag aaccgccaga aataacatca gggtatgtaa acgtttcatt
      121 gtaaatattc ttccgttgta aatggcgctg gaaaatttct catcagtgaa accttccgga
      181 aatttccccc ccttgtatga tgtatatcgc cagtatttta tttcatcccg gatataattt
      241 ttatatttcc cggcacgtgc aataacgctg accagagata taaggctcat taataacttt
      301 acaggtaatt gcataattaa atttccagtg agtgaattga caggtgaggg gtaaaacttt
      361 ctctcaggta atatatatct gaaaatattt tcaataacac cagctaggaa aaacaccacc
      421 acaggcggtg gcgtttcttt ggagcgtctt tatttaatcc gggccatcgc gtttctcagt
      481 gccaaatctg cctcttccgt tatctccaga cttttctgca acaccgtttc tgaccgggac
      541 agtacctgac gtgctgccag cggtttccgg atatgctcca tcgtcagcat cgtgtcttca
      601 tagccctgct gacgccaggc gtcggtatgt gccaacgtga aactgagcag accgccgctg
      661 ttgtcaccat cacctgcata tttcagc
//
LOCUS       DAJTYI010000318          677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYI010000318 DAJTYI010000000
VERSION     DAJTYI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..649)
                     /locus_tag="OO564_004129"
     CDS             complement(<1..649)
                     /locus_tag="OO564_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HCR6655001.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQ"
BASE COUNT          150 a          170 c          166 g          191 t
ORIGIN      
        1 gttgcaacat tttgttgttg ctgtcgccac gccagtaagc ccctgccgtt ttcattagtt
       61 tgaaatgatg gcagaaacgc atgttcggta cgtgcggacc gcggcacata tcgacatatt
      121 cttcatggaa gtacagacct ggcttgtcat catgggcgat gttttcgtca agaatggaga
      181 ctttgtagct ctccccacgg ttggcgaaag tttcacgcgc ttcgtgccag ctgactttct
      241 tcttaatgac gtcgtagttt ttctcagcaa gctcatgcat ccgcttctcg agtgcttcga
      301 catcttcctg ggttaacgtg cggtcaagat caacgtcgta ataaaaaccg ttgtcaataa
      361 ccgggccgat tgccattttg gtatgcggcc aaagttgttt aatcgcatgc cctaacaggt
      421 gcgcacagga gtgacgaatg atctccagac cttcttcgtc tttggcggta atgatcgaca
      481 gttgtgcgtc gttttcaatc agatcgcaag catcaaccag ttcgccatta acgcgccctg
      541 cgatacaggc tttcgccaga cctggaccaa tgtccagcgc aacatccatg gggcttacag
      601 cgtggtcgta atggcgttgg ctgccatcag gaagagttat aacaggcatt ttatatcctt
      661 atttgcagtg gtgaccc
//
LOCUS       DAJTYI010000319          676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYI010000319 DAJTYI010000000
VERSION     DAJTYI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>676
                     /locus_tag="OO564_004130"
     CDS             <1..>676
                     /locus_tag="OO564_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6655002.1"
                     /translation="KPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINT
                     AFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQS
                     EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYN
                     VLYETGR"
BASE COUNT          174 a          172 c          188 g          142 t
ORIGIN      
        1 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
       61 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
      121 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
      181 gtgtgatgtc atcgttcagg caccacttcc ccctaaaccg atcgaacgcg gttatgccag
      241 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
      301 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
      361 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
      421 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
      481 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
      541 cctgccggca gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct
      601 ccacctggca aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact
      661 gtacgaaacg ggccgg
//
LOCUS       DAJTYI010000320          673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYI010000320 DAJTYI010000000
VERSION     DAJTYI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..527
                     /locus_tag="OO564_004131"
     CDS             <1..527
                     /locus_tag="OO564_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655003.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
     gene            complement(590..>673)
                     /locus_tag="OO564_004132"
     CDS             complement(590..>673)
                     /locus_tag="OO564_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6655004.1"
                     /translation="RVQWAKDGALTPEEVQQVMDLLQKLLK"
BASE COUNT          204 a          111 c          152 g          206 t
ORIGIN      
        1 atgcctgtga tattttgcag aaaaaacagt ctgacatacc aaaaaggatt aaaggttata
       61 ttcgccaggc gtttggtgac gtatctttcg gttatgcctt attggaggat gtagtaagca
      121 aattaaaacg gggtaaattt gaactccaaa taccaggcgg tggaattaaa gaatgtgatg
      181 gatggtatat ttacgaaaaa ataattgatg ataattttgc catagtcata gaatctttgg
      241 gctttgcgct taaaatctat ggaggggatg aacgttttcg taatggttcc tctgttgttt
      301 tggaggatga agattactct cttatttata attttctggt taatgcaggt tgccaacaag
      361 tcgaattagc cgaacaagtg gatgcgatag taagtgcgaa tctggccgca gatagtaata
      421 ttaccaaaga aaagatttgc gagaaatata aatcgatcat tgaggcgttt aaaaaagagc
      481 aactagcgtt accagtatta gttcggtgta agaactcaga gacttaattc ccctatccca
      541 tagataacga taggggaaaa ttatttttcc agaatttatg ttattgccat tatttgagca
      601 acttttgcag caggtccatc acttgctgca cctcttccgg tgtgagcgcc ccgtctttgg
      661 cccattgcac gcg
//
LOCUS       DAJTYI010000321          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYI010000321 DAJTYI010000000
VERSION     DAJTYI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..329)
                     /locus_tag="OO564_004133"
     CDS             complement(<1..329)
                     /locus_tag="OO564_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6655005.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMA"
     gene            complement(301..>659)
                     /locus_tag="OO564_004134"
     CDS             complement(301..>659)
                     /locus_tag="OO564_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655006.1"
                     /translation="LGEAAKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCG
                     QLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPG
                     SGYGSSADAYYIAMGY"
BASE COUNT          182 a          160 c          129 g          188 t
ORIGIN      
        1 gccataccta aatcaaccgc atcctgaagc ggagctatta cattcgttgc ctctgctatc
       61 agtgcagatt tcttttcatc atttattaag gctaattgtt ctggtgttgg agaaggacgg
      121 tcaacaatga caggatgacc gttctcacca caggtaatca tttttgttac actctgtgca
      181 ttaatcagtg ttttccactc tgattccgaa atttccactg catcatcagg aatattggtg
      241 ccgtgaaatt cagtcgaata aaatccgttt gtcgatgcag aataaaaata tgacattttg
      301 ttaatatccc attgcaatgt aatacgcatc agcagatgaa ccgtatcctg aaccgggcga
      361 cacagtaata ttaaatccag tgttctggac acttgtcgcc gatacagtcg gctgcccagc
      421 cgcgccagta acgttcaatg taactacgac agcaatgact cttgagaacg tcatcgggaa
      481 cctcatcccg tatgttgaag atacattcgg ggcaccattc aactgaccgc actgtaaaat
      541 taatccagac ggaaattttt gcgcagcagc cgttacggta ttaattgacg cgaacaggct
      601 catatcaggt atctgattcg cccctgtacc tacattcctt ttagctgctt ctcccaaac
//
LOCUS       DAJTYI010000322          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYI010000322 DAJTYI010000000
VERSION     DAJTYI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            77..316
                     /gene="relB"
                     /locus_tag="OO564_004135"
     CDS             77..316
                     /gene="relB"
                     /locus_tag="OO564_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6655007.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            316..603
                     /gene="relE"
                     /locus_tag="OO564_004136"
     CDS             316..603
                     /gene="relE"
                     /locus_tag="OO564_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6655008.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          174 a          133 c          172 g          179 t
ORIGIN      
        1 ttccttcgtg acttgcccta agcatgttgt agtgcgatac ttgtaatgac atttgtaatt
       61 acaagaggtg taagacatgg gtagcattaa cctgcgtatt gacgatgaac ttaaagcgcg
      121 ttcttacgcc gcgcttgaaa aaatgggtgt aactccttct gaagcgcttc gtctcatgct
      181 cgagtatatc gctgacaatg aacgcttgcc gttcaaacag acactcctga gtgatgaaga
      241 tgctgaactt gtggagatag tgaaagaacg gcttcgtaat cctaagccag tacgtgtgac
      301 gctggatgaa ctctgatggc gtattttctg gattttgacg agcgggcact aaaggaatgg
      361 cgaaagctgg gctcgacggt acgtgaacag ttgaaaaaga agctggttga agtacttgag
      421 tcaccccgga ttgaagcaaa caagctccgt ggtatgcctg attgttacaa gattaagctc
      481 cggtcttcag gctatcgcct tgtataccag gttatagacg agaaagttgt cgttttcgtg
      541 atttctgttg ggaaaagaga acgctcggaa gtatatagcg aggcggtcaa acgcattctc
      601 tgaaccaaag catgacatct ctgtttcgca ccgaaggtga cacttctgct ttgcgttg
//
LOCUS       DAJTYI010000323          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYI010000323 DAJTYI010000000
VERSION     DAJTYI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..345
                     /locus_tag="OO564_004137"
     CDS             <1..345
                     /locus_tag="OO564_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6655009.1"
                     /translation="FLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKS
                     PVFSLGNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSN
                     VLIARLAAELLV"
     gene            417..>653
                     /gene="ppnP"
                     /locus_tag="OO564_004138"
     CDS             417..>653
                     /gene="ppnP"
                     /locus_tag="OO564_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6655010.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEF"
BASE COUNT          155 a          143 c          174 g          181 t
ORIGIN      
        1 tttcttgagc cgtcgcgaat attgcctcca ctggtttcct ctattgttga agatcatcag
       61 gtgggggtta tcgttccggt tgaggagatg ctgcccgttc aggcgcaaaa atggcaaatt
      121 ttgcagaaat cgccggtatt ttcattgggt aaccccattc atgattcaga acaaaaaatc
      181 attgatgctg ggaaagaatt actggcaaaa ggggctgatg tcatcatgct ggattgttta
      241 ggatttcacc aacgtcatcg cgatttactg caaaaacagc tcgatgttcc tgtcttgctc
      301 tctaacgtat tgattgcacg gctggctgcg gaattactgg tgtaattttg cgtgacagcc
      361 agcgcctctg gcccctatag tgaagtagat gttcaactac cagacagggc cagtttatgc
      421 ttcaaagtaa tgagtacttt tccggcaaag tgaaatcgat cggcttttcc agcagcagca
      481 ctggtcgcgc cagcgtgggt gttatggttg aaggcgaata caccttcagc accgctgagc
      541 cggaagagat gacggtaatc agtggcgcgc tgaatgtgtt actgcctgac gcgaccgact
      601 ggcaagtgta tgaagccggt tcggtgttta atgttcccgg tcacagtgag ttt
//
LOCUS       DAJTYI010000324          651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYI010000324 DAJTYI010000000
VERSION     DAJTYI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>651)
                     /gene="ipaH4.5"
                     /locus_tag="OO564_004139"
     CDS             complement(<1..>651)
                     /gene="ipaH4.5"
                     /locus_tag="OO564_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6655011.1"
                     /translation="CLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPD"
BASE COUNT          200 a           94 c          161 g          196 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac aatggattcc
       61 ctgaaatgtt aacattcgta aaaatgggca atgtgattat attttcgggt aatgaagtta
      121 gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat aatctgcgta
      181 gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt agttcctgta
      241 gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc aagacagtaa
      301 ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt aacgtctcca
      361 gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc agacatgaca
      421 aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc gggagtatat
      481 tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca ggtagggaga
      541 ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt ggtaaagaac
      601 gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat a
//
LOCUS       DAJTYI010000325          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYI010000325 DAJTYI010000000
VERSION     DAJTYI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            163..>643
                     /locus_tag="OO564_004140"
     CDS             163..>643
                     /locus_tag="OO564_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6655012.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     "
BASE COUNT          161 a          148 c          169 g          165 t
ORIGIN      
        1 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
       61 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      121 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      181 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      241 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      301 aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga tgtttgtcgt
      361 ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc actcacagca
      421 aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg taatacgacc
      481 tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac ttattacgac
      541 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
      601 gctaccgtct gtattcaggc gggtactgat accgaactca acg
//
LOCUS       DAJTYI010000326          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYI010000326 DAJTYI010000000
VERSION     DAJTYI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>641
                     /locus_tag="OO564_004141"
     CDS             <1..>641
                     /locus_tag="OO564_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6655013.1"
                     /translation="VGAFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVSFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGAAALNHSGKVDTHNFYGTDSDYDDSTTDTATMR"
BASE COUNT          141 a          185 c          172 g          143 t
ORIGIN      
        1 gcgtgggtgc gttttttgcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg tattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggcg cagcgcaccg acaggctcga tcaatatgat cagcaagcag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gatactaccg cggtgcgcct gaatgtgatg ggcgaaaaaa
      361 cccacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtct
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc actcagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gagcggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg t
//
LOCUS       DAJTYI010000327          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYI010000327 DAJTYI010000000
VERSION     DAJTYI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..156)
                     /locus_tag="OO564_004142"
     CDS             complement(<1..156)
                     /locus_tag="OO564_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655014.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQA"
BASE COUNT          163 a          139 c          126 g          207 t
ORIGIN      
        1 cgcctggatg cgattttctg ttgttgttcc ttccctagcc cagtcatccc agatgcggtg
       61 gtaatctctg gtacactgtt cttctaccga taaacgagat atacatgata agccacaaag
      121 ggaacgaaaa aaagaatgat tgttgatcgg tttcatttat taacccctca ctattcatat
      181 cttagtctat taatgcaaat aaaacgagaa agtctttaaa aaaacaatcc tagagcttta
      241 tcggcgtttt cattccggct ggaggaatat gttcctgtct ttccagtaac cgtcgattgt
      301 tatatcagtc tacccatgtt aatgtgacca gttctacttt catccagttt ggattgatct
      361 ttatccccta tattttcaaa caatttttat catttaactc attactgtct cgatattccc
      421 atagcaaacg atctggttgg ttatcccctc aaaaaagtca gttgcacgcc gtaaatccct
      481 gccggtattg agcgaggatt tacgggttgt tgttaaggtc cgtaatcggg tcacagtttt
      541 tccggagtca atccggtctg cggtttatgc ttatgcgacg tgattatgaa tggtgcagtc
      601 gtgagccgtt ttcagacaat cgcagggcca gtacc
//
LOCUS       DAJTYI010000328          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYI010000328 DAJTYI010000000
VERSION     DAJTYI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>634
                     /gene="pyk"
                     /locus_tag="OO564_004143"
     CDS             <1..>634
                     /gene="pyk"
                     /locus_tag="OO564_004143"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6655015.1"
                     /translation="VICKVLNNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEKG
                     VDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGIMVA
                     RGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANA
                     ILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDRVMNSRLEFNNDNRKL"
BASE COUNT          176 a          165 c          158 g          135 t
ORIGIN      
        1 gttatctgta aagtgctgaa caacggtgac ctgggcgaaa acaaaggtgt gaacctgcct
       61 ggcgtttcca ttgctctgcc agcactggct gaaaaagaca aacaggacct gatctttggt
      121 tgcgaaaaag gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc
      181 gaaatccgtg agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc
      241 gaaaaccagg aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg
      301 gttgcgcgtg gcgacctggg tgtagaaatc ccggtagaag aagttatctt cgcccagaag
      361 atgatgatcg aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg
      421 gattccatga tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc
      481 atcctcgacg gtactgacgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg
      541 ctggaagcgg tttctatcat ggcgaccatc tgcgaacgta ccgaccgcgt gatgaacagc
      601 cgtctcgagt tcaacaatga caaccgtaaa ctgc
//
LOCUS       DAJTYI010000329          629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYI010000329 DAJTYI010000000
VERSION     DAJTYI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..609
                     /locus_tag="OO564_004144"
                     /pseudo
     CDS             1..609
                     /locus_tag="OO564_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          149 a          146 c          181 g          153 t
ORIGIN      
        1 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
       61 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
      121 ggctgaatgc cgacaacgac ggtgtcagtc agatgattaa agactcgcgc attgctgctg
      181 tcacggtgac cggaagtgtt cgtgctggag cggctattgg cgcacaggct ggagcggcac
      241 tgaaaaaatg cgtactacta tccgccaacg gttctggcga atgttacccc agaaatgacc
      301 gcgtttcggg aagaaatgtt tggccccgtt gctgcaatca ccattgcgaa agatgcagaa
      361 catgcgctgg aactggctaa tgatagtgag ttcggccttt cagcgaccat ttttaccacc
      421 gacgaaacac aggccagaca gatggcggca cgtctggaat gcggtggggt gtttatcaat
      481 ggttattgtg ccagcgacgc gtgagtggcc tttggtggcg tgaaaaagag tggctttggt
      541 cgtgagcttt cccatttcgg cttacacgaa ttctgtaata tccagacagt gtggaaagac
      601 cggatctgac gcgacatatt cagctctga
//
LOCUS       DAJTYI010000330          629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYI010000330 DAJTYI010000000
VERSION     DAJTYI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..568)
                     /locus_tag="OO564_004145"
     CDS             complement(<1..568)
                     /locus_tag="OO564_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6655016.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVE"
BASE COUNT          147 a          164 c          157 g          161 t
ORIGIN      
        1 tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac agcacattca
       61 gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat gcgatagatt
      121 ttgccctgcg cccacggcta ccgtccggat aaatatgctc ggtgtaaaat ccggcctgaa
      181 tggttaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatcc cgggttctgc
      241 gtgtcgcaac ggtattcaga tattgctctg cggcttcgct cagagctggc gtatgttccc
      301 gacctactga ttcgcacagg tactcaacga agcctgatac cagttctcgt tctcgaggcg
      361 tgatcgcccc accgaccgga gtccggtaat cgaatcccag ttgcaggagt ttgaaaaaac
      421 gcttgtggaa tgcgtagtta cgaacgcgct taaaatcagc gtgtatccac tcgcctgttt
      481 tgatttgatg cagaaattcg caactctccg acgtcgccgg gagaagtaat ccggaagagg
      541 tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt gttactcagc
      601 agggggtcaa ccctgcactg aattgtaga
//
LOCUS       DAJTYI010000331          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYI010000331 DAJTYI010000000
VERSION     DAJTYI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..255
                     /locus_tag="OO564_004146"
     CDS             <1..255
                     /locus_tag="OO564_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6655017.1"
                     /translation="NDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDK
                     EKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            279..>621
                     /locus_tag="OO564_004147"
     CDS             279..>621
                     /locus_tag="OO564_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6655018.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQ"
BASE COUNT          169 a          135 c          172 g          145 t
ORIGIN      
        1 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
       61 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      121 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      181 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      241 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      301 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      361 taaacacccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      421 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      481 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      541 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      601 aaatggtggc gcaggcgcag c
//
LOCUS       DAJTYI010000332          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYI010000332 DAJTYI010000000
VERSION     DAJTYI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>621)
                     /locus_tag="OO564_004148"
     CDS             complement(<1..>621)
                     /locus_tag="OO564_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCR6655019.1"
                     /translation="MMYINLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSL
                     VWATVCITVLSFIVWLHHFFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGR
                     IVFHSAMLWTIGFIVTFSVGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGC
                     FAGMTYWWPKAFGFKLNETWGKRAFWFWIIGFFVAFMPLYALGFMGM"
BASE COUNT          181 a          172 c          165 g          103 t
ORIGIN      
        1 catgcccatg aagcccagcg catacagcgg cataaaggca acgaagaagc cgatgatcca
       61 aaaccagaac gcgcgtttac cccaggtttc gttcagtttg aaaccgaacg ctttaggcca
      121 ccagtaggtc atccctgcga agcagccgaa gaccacgccg ccgatgatca cgttatggaa
      181 gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc gcgcccggta ctgccagcag
      241 cacgccagtc ataccgccca ccgagaaggt gacgataaaa ccgatagtcc acagcatcgc
      301 agaatggaac acgatgcggc cctgatacat ggtgaacagc cagttgaaga tcttcacccc
      361 ggtcgggatg gcgataatca ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc
      421 ccccatcgta aagaagtggt gcagccaaac gatgaacgac agcacggtga tgcagacggt
      481 tgcccatacc agcgaggtat aaccaaacag acgtttacgc gagaaggttg ccgcaatttc
      541 ggagaacaca ccgaaaacag gcaggatcag gatgtaaact tccgggtggc cccaggccca
      601 aatcaggttg atgtacatca t
//
LOCUS       DAJTYI010000333          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYI010000333 DAJTYI010000000
VERSION     DAJTYI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            177..242
                     /gene="yobI"
                     /locus_tag="OO564_004149"
     CDS             177..242
                     /gene="yobI"
                     /locus_tag="OO564_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6655020.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            381..>617
                     /locus_tag="OO564_004150"
     CDS             381..>617
                     /locus_tag="OO564_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6655021.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTN"
BASE COUNT          188 a          123 c          114 g          192 t
ORIGIN      
        1 tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct atacatgtaa
       61 agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg tcattccatt
      121 gaaatagata cgaatatacg taatagcata aaaaaattat tttttcagga gtcgcaatgt
      181 atatttttat tacgcatttc ttcactgaat atgtaatatt caaatatttg cttccaatat
      241 aacctgttga ataaattata ctgtgccatt tttcagatca tcgggacacc tcgcaagttt
      301 tcttcatcct acgctggata tctatccagc atttttttat catacagcat tatctttgat
      361 tcattacgca ggagcgtcat gtgataggca gactcagagg catcatcatt gaaaaacaac
      421 ccccgctggt gttaattgaa gtgggcggcg taggctatga agtgcatatg ccgatgacct
      481 gtttttatga actccctgaa gcgggtcagg aagcgatcgt tttcacccac tttgtggtgc
      541 gtgaagacgc gcaactgctg tacggtttta acaataaaca agaacgcacg ttgttcaaag
      601 agttgatcaa aaccaac
//
LOCUS       DAJTYI010000334          613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYI010000334 DAJTYI010000000
VERSION     DAJTYI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..265)
                     /locus_tag="OO564_004151"
     CDS             complement(<1..265)
                     /locus_tag="OO564_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655022.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLR"
     gene            complement(267..545)
                     /locus_tag="OO564_004152"
     CDS             complement(267..545)
                     /locus_tag="OO564_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655023.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT          142 a          163 c          176 g          132 t
ORIGIN      
        1 ggcgcaacag ccagtcagac aatgcgccag caccaccagc agcacgaatc acccgtgcgt
       61 tactgaaaaa cggcagcaat gttttgtctt ccactagcgg ctggcgaacg gcaggaacga
      121 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtacc cgggtattgt
      181 ggaataccgg catggattca cggcctggct taaggaccac cagcccgagt tccggaacca
      241 gaacaggtcg aagtaatacc cgcacattac ctccagatcc gttgctggaa tgtgcaggac
      301 ggacgcggtg gccgttcgga ataagggagc ctgacgaaga ttatccagtg acgataatcg
      361 agactgaggg ctttcttaat ctcgtatccg tgtctgcggt agcgctgaat cagccattca
      421 gcctgttctt cggtgcagga gtcgtgctga taccagtcag atttgaatgc atgagaacgc
      481 cgcccgtgcc tgctggcagg ggcggcagag ttatccgaat tgtaaaattt ggtatcgtgc
      541 gccatctgtt ttctctgctg gcgcagcagg tgccagttgt tcaggctgac ggatggattg
      601 taaaccagaa cga
//
LOCUS       DAJTYI010000335          612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYI010000335 DAJTYI010000000
VERSION     DAJTYI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..353
                     /locus_tag="OO564_004153"
     CDS             <1..353
                     /locus_tag="OO564_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6655024.1"
                     /translation="NIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARNHLGIVFAP
                     DKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYVLDEMLREIRHQLAQ
                     SQQLRPEQAAESED"
     gene            complement(350..>612)
                     /locus_tag="OO564_004154"
     CDS             complement(350..>612)
                     /locus_tag="OO564_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655025.1"
                     /translation="KLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHTDL
                     SALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
BASE COUNT          149 a          151 c          181 g          131 t
ORIGIN      
        1 cgaatatctg gcgcttcaac gtcgccggga gtaaagaggt gcgggattat cgcgtgaagg
       61 gaaattacgt ttgcgataac acgcaatcgg cgctggagct ggcacgaaat cacctgggga
      121 ttgtgtttgc gccagacaaa agtgtgcaga gcgatctgca agacggcacg ctggtgccct
      181 gctttcagca cccgtatgaa tggtggctgg atctggtggc tatcttccgt aaacgggaat
      241 acctgccctg gcgggtccag tatgtgctgg atgaaatgct gcgtgaaatt cgccaccagc
      301 ttgctcagtc gcagcaactg cggcctgaac aagcggcgga aagcgaagat taatgatcga
      361 gataaaggta atgcacccag ctggtcatgc gtaatagcac tttgcgcatc accgagacat
      421 ggctgaaatg atcagagtag accgccacct gggcgtaaat accgtctggt aaagcgctca
      481 aatcagtgtg ctcatccagt tcaatggtcg cgataacgcc atctgaaccc ggcgctgtgt
      541 ttaacgtctg taaggtgccg gtcgactgat aagctccgcc gggaacagct ggactaatgg
      601 ctgccagctt gc
//
LOCUS       DAJTYI010000336          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYI010000336 DAJTYI010000000
VERSION     DAJTYI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..563)
                     /gene="uidR"
                     /locus_tag="OO564_004155"
     CDS             complement(<1..563)
                     /gene="uidR"
                     /locus_tag="OO564_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6655026.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRA"
BASE COUNT          146 a          155 c          144 g          161 t
ORIGIN      
        1 atcgcgcgta atccctgagc aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg
       61 gccagtacac cgtaggtcag atccagcagt aaacgtgaag tcattgccgt gttgatgtct
      121 gggcttatct cgcctttttg ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc
      181 gtcatatgct tatttttaag catggcggcg acctgtgggt tacgcatccc ttccgccata
      241 atttcgacca ccagcgcccg ttgcccaaag gcttcatggg tgagagagac aatagactcg
      301 accatatagt caacaaaatg aatcccttca atcggttccc ggaaacgagc cagcgccctc
      361 tcctggtcct gtaagataat cgcctgaatc aaggcttctt tggagatgaa atggtgatag
      421 agcgtcccag gactaatggc gcaagattta cagatggctt tcatcgaggc actgtgaaat
      481 ccattttctg aaaaaatctc tctggcagca ttgaggatcc gggtccgtgt cggttgtgct
      541 tcagtctgca tgttatccat cattaaacca ttatagttag cgtgaataac tagtttaccg
      601 cgttgt
//
LOCUS       DAJTYI010000337          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYI010000337 DAJTYI010000000
VERSION     DAJTYI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            51..>598
                     /gene="wcaF"
                     /locus_tag="OO564_004156"
     CDS             51..>598
                     /gene="wcaF"
                     /locus_tag="OO564_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6655027.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
BASE COUNT          167 a          126 c          152 g          153 t
ORIGIN      
        1 caggtaagac taaagcctta tataacaaag tctgaatata aggaaaacca atgcaagatt
       61 taagcggttt ctcggtgccg aaagggttcc ggggcggcaa cgctattaaa gtgcaattat
      121 ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat cgctggcggg
      181 cttttttatt acgtttattc ggagcaaaaa taggaaaaaa cgtagttatt cgtccgtcag
      241 taaaaattac ctatccgtgg aaattaacct taggtgatta cgcgtgggtt ggcgatgacg
      301 tcaatttata taccctcggt gaaataacca ttggcgcaca ttcggtgata tcgcaaaaaa
      361 gttatttatg caccggtagc cacgaccatg caagtcaaca tttcaccatt aacgccacgc
      421 ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc ccaggcgtca
      481 caatcggcga cggcaccgtc gtgggtgcac gaagcagtgt ttttaaatcg cttccggcaa
      541 atgtggtttg ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa actgaata
//
LOCUS       DAJTYI010000338          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYI010000338 DAJTYI010000000
VERSION     DAJTYI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>594)
                     /gene="corA"
                     /locus_tag="OO564_004157"
     CDS             complement(<1..>594)
                     /gene="corA"
                     /locus_tag="OO564_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6655028.1"
                     /translation="EDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDG
                     NAYELLLDLFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEYDEALSTLAELEDIG
                     WKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFLMQA
                     AMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFE"
BASE COUNT          144 a          170 c          148 g          132 t
ORIGIN      
        1 ctcaaagttc atcccgtagc tggaagcaac cagcgttggc ggcaggaaca cgacggatac
       61 caccgagaaa attttgatga tgcggttctg ctcgatgttg ataaaaccca ttgccgcctg
      121 catcaggaag ttcaccttct ggaacaggga ttcgttatgc ggcagcaggg attcgatatc
      181 gcgcaggatt tcacgcgcct gctccagttg cccacccggt aaacgcgctt tacgcaccag
      241 gaagttgaga gcgcgctggg tatccatcag acacaggcga actttccagc cgatatcttc
      301 cagttccgcc agcgtggaga gcgcctcgtc gtactcatcg ccctgatgcc cttccataat
      361 cacccggctc aactgctcca ggtcgctata gatattttca atttcatctg ccaactgttc
      421 gattttggtt tcgaacagat ccagcagcaa ctcgtaggcg ttaccgtcta ccatcgactg
      481 gctacgggca cgcatacgat acagacgaaa agcgggcagt tcacgctcac gcagagtaaa
      541 cagacgacca tcacggatgg taaatgccac agtggagtta ccggcgtgat cttc
//
LOCUS       DAJTYI010000339          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYI010000339 DAJTYI010000000
VERSION     DAJTYI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..87)
                     /locus_tag="OO564_004158"
     CDS             complement(<1..87)
                     /locus_tag="OO564_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6655029.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGF"
     gene            complement(100..>590)
                     /locus_tag="OO564_004159"
     CDS             complement(100..>590)
                     /locus_tag="OO564_004159"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="catalyzes the formation of
                     2-hydroxy-3-oxopropanoate (tartronate semialdehyde) from
                     two molecules of glyoxylate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6655030.1"
                     /translation="CAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAY
                     LGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAP
                     AFEQAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMH
                     YE"
BASE COUNT          168 a          154 c          122 g          146 t
ORIGIN      
        1 aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac caaataacat
       61 cgataaatta gcagagaaac gtaacatcat ttatctccct tattcatagt gcatgaagca
      121 ggtttcagtc ggtgcatccg ctgcgttatc ggcgatatct tcaaattcca tgacgttatc
      181 cagttcgctg cccatcgaaa tattggtcac acgctcgaga ataacttcca ctactaccgg
      241 tacccgatat tgcgccatta aggctttcgc ctgttcaaag gctggcgcaa tatcttccgg
      301 tttgaagacc cgaatagctt tacaacctaa accttccgct acttttacgt ggtcaacacc
      361 gtagccattc acttcactgg agttgatatt ctcgaaagcg agttgcacgc agtagtccat
      421 gtcaaaagcg cgttgtgact gacgaatcag gccgagatag gcgttattca ccagcacatg
      481 gatgtacgga atgttgaact gcgcgccaac ggctaactct tcaatcagga actggaagtc
      541 aaagtcgcca gaaatcgcca ccacattgcg tttcggatca gcggcacaaa
//
LOCUS       DAJTYI010000340          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYI010000340 DAJTYI010000000
VERSION     DAJTYI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(264..470)
                     /locus_tag="OO564_004160"
     CDS             complement(264..470)
                     /locus_tag="OO564_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6655031.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          167 a          130 c          132 g          149 t
ORIGIN      
        1 ttaccctgaa gtttcacgaa agtgtgtaag gaagtatgca atctaactgg agatttctga
       61 tgatctagtc gcttagagcg acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg
      121 atactattga aacgtgatgc taaaacagtg atgaaatgaa taatacgatg gaaacgtctt
      181 ccatgttaat tatcatggaa gacgttgatg ttgaacacct aaaccagccc cctgatacga
      241 ggctggctta agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc
      301 atccacaccg gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg
      361 gcgcggtctt tccagaaaaa catgaaaggc agcgccagaa cgcccgctgc aaccgcatat
      421 gtacggcgta aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc
      481 cccttttgta tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa
      541 tagtaccgcc ttttgtgtaa ttttaccact catccgac
//
LOCUS       DAJTYI010000341          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYI010000341 DAJTYI010000000
VERSION     DAJTYI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..319
                     /locus_tag="OO564_004161"
     CDS             <1..319
                     /locus_tag="OO564_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR6655032.1"
                     /translation="GDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVE
                     EKSTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK
                     EVK"
     gene            complement(360..>543)
                     /locus_tag="OO564_004162"
     CDS             complement(360..>543)
                     /locus_tag="OO564_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6655033.1"
                     /translation="FSLKTDDILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFL
                     RYFLRGLAARQHVKKS"
BASE COUNT          163 a          123 c          129 g          128 t
ORIGIN      
        1 aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc
       61 aaccaccaaa gaagatgctg ccaagacact tgagccgtta agcgccaaat acaaaaacat
      121 cgcgggtgtt gaagaaaaat caacctatac cgatacctac gcgcaggaaa acgtgactat
      181 cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc
      241 tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg
      301 ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc atacccgtct
      361 tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga
      421 tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt aatttccggc
      481 atcgaaacgc ggaactgctg ttcggtgagg atagccagaa tgtcatcggt tttcagcgaa
      541 aac
//
LOCUS       DAJTYI010000342          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYI010000342 DAJTYI010000000
VERSION     DAJTYI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            23..>543
                     /locus_tag="OO564_004163"
     CDS             23..>543
                     /locus_tag="OO564_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655034.1"
                     /translation="MDCYAEHEEGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNVF
                     CNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIAA
                     PECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVLP
                     DEYIAPEFKGMSGA"
BASE COUNT          172 a          106 c          111 g          154 t
ORIGIN      
        1 tagtgcatta cctgtatggg caatggactg ctatgctgaa catgaagagg gaaataccgt
       61 agtcattggt tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag gtgataagat
      121 ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat aatgcattac ctgccccatc
      181 acctggagaa gaatacccat cagcctggac taatataatg atgtttttac caggcggtca
      241 ggacttttat aatcagaact cctatatttt tggcgtaaca tacaatggag ttgattatga
      301 ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt gatataaaag gagcgggtac
      361 ctttaataat cactataaaa accccgctgt ctgtagtgga ggtcctgagc ctcaattatc
      421 agtaaccttt ccagctcgcg tacaactcta tattaaatta gcaaaaaatg ccaatagagt
      481 taataaaaac ctggtgttac ctgatgaata tatcgctcca gagtttaaag gtatgagtgg
      541 cgc
//
LOCUS       DAJTYI010000343          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYI010000343 DAJTYI010000000
VERSION     DAJTYI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..513
                     /locus_tag="OO564_004164"
     CDS             <1..513
                     /locus_tag="OO564_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6655035.1"
                     /translation="SAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNFQPDTLKM
                     VYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVIKRTYTGE
                     EQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKVESTDTSS
                     LPVTFPEQPE"
BASE COUNT          182 a          110 c          131 g          120 t
ORIGIN      
        1 tctgctggta atcctgaaac aaaagagcaa taccagctaa cgaaacaatt taacatcaaa
       61 tggctttata cagaggatgg gaaaaactgg tatgaggaac aaaagaactt tcagcctgat
      121 acgttgaaaa tggtctatga ccacaacggc gttattattt gtattgaaaa ggatgtttca
      181 gcaattaatc cggaaggcgc aagcgtcgtt gaggttcctg atattacagc aaatcgccgg
      241 gctgatattt caggtaaatg gatgttcaaa gatggcatag tgataaagcg aacttatacc
      301 ggggaagagc agaggcaaca agtggaaaat gaaaagcaaa gcctgttgca acttgtcagg
      361 gataaaaccc agctatggga ctcacagcta cgactgggca tcatttccgc cgggaataag
      421 cagaaattaa ccgagtggat gctctacgcg cagaaggtcg aatccacaga cacctccagt
      481 ctgccagtaa cgtttcccga acaaccagaa tgagagaagg cccgctatcg ggccttaatt
      541 ttt
//
LOCUS       DAJTYI010000344          542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYI010000344 DAJTYI010000000
VERSION     DAJTYI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..428)
                     /locus_tag="OO564_004165"
     CDS             complement(<1..428)
                     /locus_tag="OO564_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6655036.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSAL"
BASE COUNT          131 a          154 c          129 g          128 t
ORIGIN      
        1 agtgcagaac aggatgacat cagcgtctgc gccgtcgccg cacctgatgt ggggtaagag
       61 agttctcccg cttcgacaaa cttcaggtca aagcggacaa tacgcccttc actcttcgat
      121 gtgctgaccc gaacttctcc gtcaacacag actttcagct cgccgtatgt cggatggaca
      181 agcgtgccgg gaccgggttt attcagcgcg tcaatcaggc gatcgcgctg gtcaaagcag
      241 tcatctccca ccacataagc cgtgatggac gggcggaaag tgacttttcc cagatcttcg
      301 gtatagggtt tgtcgcggtt cgggtattcg tgcgtttcca cacgacgacc ggttcccgca
      361 ttttcttctt caaccttaaa cggcacaccg cgaaatgacg cgtcctgaag tctgtctttc
      421 cacgtcatat aaactccgta cataaaaaat cccaccggag tgggactcat taacagatta
      481 atttttcatt acctgccaaa gcgcgtatag ccaacatcat ggctgacatc aaaaccgctg
      541 ga
//
LOCUS       DAJTYI010000345          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYI010000345 DAJTYI010000000
VERSION     DAJTYI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..182)
                     /locus_tag="OO564_004166"
     CDS             complement(<1..182)
                     /locus_tag="OO564_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6655037.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKAS"
BASE COUNT          154 a          100 c          121 g          166 t
ORIGIN      
        1 gatgctttga atgtgaatct tgcattcgcg aagaagctaa agctcacagc tactacgaaa
       61 cctgtgaagt ttgccagggc ctgactggta tgcgcggcat agatacatac accaaaaact
      121 acccagtgta taagtgtatt cagcacacct atagatgtgt actttacaaa taactttaac
      181 atttatttaa tcaatgagtt ctgaaagtca tgaagtctat catccaagtc tcaattgatc
      241 gatatttgct gtgtctgatg agacaaaact gagacacata aggcctcgca atggcttgca
      301 aggctttaca tgttttgatt tagtgggacg tgtgagcgca gtgttgatgg ggtaatgctt
      361 tgaattagaa gcggattctt ataattcgta atgcgaaggt cgtaggttcg actcctatta
      421 tcggcaccat ttaaatcaat aagttacaca tcattagtac cttccttatt tcttgactgg
      481 gacaaatttg ggaccgatgg gttcagaatc gagtctattt gccgtgcgtg ttcggtaagg
      541 t
//
LOCUS       DAJTYI010000346          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYI010000346 DAJTYI010000000
VERSION     DAJTYI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..166
                     /locus_tag="OO564_004167"
     CDS             <1..166
                     /locus_tag="OO564_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="cytoplasmic mutarotase that catalyzes the
                     conversion between beta-pyran and beta-furan forms of
                     D-ribose; RbsD is required for efficient ribose
                     utilization in E. coli; rbsD-mutant E. coli strains are
                     unable to use ribose as a sole carbon source; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6655038.1"
                     /translation="THLEQLQKHQGNTIEIRYTTHEQFKQQTAESQAVIRSGECSPYA
                     NIILCAGVTF"
     gene            174..>534
                     /locus_tag="OO564_004168"
     CDS             174..>534
                     /locus_tag="OO564_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6655039.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          144 a          129 c          136 g          125 t
ORIGIN      
        1 cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac
       61 gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg
      121 ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag
      181 cattacttca gcttaaaggc atcgataaag ccttcccggg tgtaaaagcc ctctcgggcg
      241 cagcgttaaa tgtctatccg ggccgtgtga tggcgctggt gggcgaaaac ggcgcgggta
      301 aatccaccat gatgaaagtg cttactggta tctatactcg cgatgccggc acgcttttat
      361 ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga
      421 ttatccatca ggaactgaac ctgatcccgc agttgaccat tgccgaaaac attttcctcg
      481 gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gtcg
//
LOCUS       DAJTYI010000347          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYI010000347 DAJTYI010000000
VERSION     DAJTYI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            23..>532
                     /locus_tag="OO564_004169"
     CDS             23..>532
                     /locus_tag="OO564_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6655040.1"
                     /translation="MFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYICD
                     KPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVF
                     ESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLN
                     KHGQWKKLSF"
BASE COUNT          110 a          155 c          157 g          110 t
ORIGIN      
        1 tactccccgc ccgctgaaac gcctgttcac agccaaccag tgctggacgt ccttcctgga
       61 tgcgggcggt ctgcgcgata tcgaggttga ggctgtcacc aaaatgctgg cctgcggcac
      121 gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata
      181 cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct
      241 gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac
      301 cctgccggac acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg
      361 ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat
      421 cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc
      481 tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct tc
//
LOCUS       DAJTYI010000348          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYI010000348 DAJTYI010000000
VERSION     DAJTYI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            58..524
                     /locus_tag="OO564_004170"
                     /pseudo
     CDS             58..524
                     /locus_tag="OO564_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          127 a          133 c          162 g          102 t
ORIGIN      
        1 cgccagcccc gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg
       61 acggcagaag aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg
      121 ggattaccag agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg
      181 tcgctgcatg aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc
      241 gaagcattca gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt
      301 ctggcggagg cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag
      361 aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg
      421 ctgatcggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg
      481 agcgtactgc cggaatggcc ttccaaccga gttgacgacc tcct
//
LOCUS       DAJTYI010000349          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYI010000349 DAJTYI010000000
VERSION     DAJTYI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>523)
                     /gene="deoR"
                     /locus_tag="OO564_004171"
     CDS             complement(<1..>523)
                     /gene="deoR"
                     /locus_tag="OO564_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6655041.1"
                     /translation="EEKRRAAKLAATLVEPDQTLFFDCGTTTPWLIEAIDNEIPFTAV
                     CYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQTLNNFCPDIAFYSAAGVHV
                     SKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPARMGDLKRFDIVVSDCCPE
                     DEYVKYAQTHRIK"
BASE COUNT          139 a          137 c          122 g          125 t
ORIGIN      
        1 aacttaatac ggtgcgtctg cgcgtacttc acatactcat cttccgggca acaatcactc
       61 accacaatat caaagcgttt caggtcaccc atgcgcgccg gacgcacctt gccaaattta
      121 ctgtggtcga caaccagcac atgcttttgc gccatcgaca tggcccagtg ttttaccggc
      181 aactcttcaa gattaaaaca ggtagcgcct ttactgacat gcacgcccgc cgcggaataa
      241 aaagcgatat ccgggcaaaa attattcaac gtttgctgaa aatcgatggg tttgaaaatg
      301 gcgttgctgg cgtgaaattc accaccgcac agaaatgcgc ggcaatgggg tttctctttc
      361 agcgccagaa aggtatttag cgaataacaa acggcggtaa aagggatttc attatcaatc
      421 gcttcaataa gccacggcgt ggtggtgcca cagtcaaaaa agagggtctg atcgggttct
      481 accagtgtcg cagccagttt tgccgcccgg cgtttttctt cca
//
LOCUS       DAJTYI010000350          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYI010000350 DAJTYI010000000
VERSION     DAJTYI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>523
                     /locus_tag="OO564_004172"
     CDS             <1..>523
                     /locus_tag="OO564_004172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="C4-dicarboxylate-sensing histidine kinase; part of
                     two-component regulatory system with DcuR; regulates
                     anaerobic fumarate respiration; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6655042.1"
                     /translation="SAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEI
                     RQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILN
                     ALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSRWSIIW
                     SVLFGMLVGLIGT"
BASE COUNT          123 a          131 c          142 g          127 t
ORIGIN      
        1 tcagcgcggt actgttctcg gtgctattgg tggtgcatct gatttacttc tcgcaaatca
       61 gtgatatgac gcgagacggg ctggccaaca aggcactggc agtggcgcgt accctcgccg
      121 actcgccgga aatccgtcag ggcttgcaga aaaaaccgca ggagagtggc atccaggcca
      181 tcgcggaagc cgtgcgcaaa cgcaacgatc tgctgtttat tgtcgttacc gatatgcaaa
      241 gtcttcgcta ctcacatcct gaagcccagc gtattggtca gccatttaaa ggtgatgaca
      301 tccttaatgc gctgaatggc gaagaaaatg tcgctatcaa tcgcggtttt ctggcgcagg
      361 ctttacgcgt atttaccccc atctacgatg aaaatcataa acaaattggc gtggtggcga
      421 tcggccttga gttaagccgc gtgacccaac agatcaatga cagtcgctgg agcattatct
      481 ggtcggtatt atttggcatg ctggtcggac tgattggcac ctg
//
LOCUS       DAJTYI010000351          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYI010000351 DAJTYI010000000
VERSION     DAJTYI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..494
                     /locus_tag="OO564_004173"
     CDS             <1..494
                     /locus_tag="OO564_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="catalyzes the synthesis of pseudouridine from
                     U-2604 in the 23S ribosomal RNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6655043.1"
                     /translation="RAGNDHEKEYLVTVDKPITDEFIRGMGAGVPILGTVTKKCKVKK
                     EAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDEL
                     IDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASNGKRFTSPGRKK
                     KGR"
BASE COUNT          148 a          118 c          151 g          101 t
ORIGIN      
        1 tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa ccgattaccg
       61 atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg acgaaaaagt
      121 gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag gggctgaacc
      181 gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg gaacgtacgc
      241 gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat ttaaccgacg
      301 atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca aaacctaaag
      361 cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg gaaaaaacgg
      421 cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg gggcgtaaaa
      481 agaagggacg ctgattaacg cctcagactg atgaaaac
//
LOCUS       DAJTYI010000352          517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYI010000352 DAJTYI010000000
VERSION     DAJTYI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..242)
                     /locus_tag="OO564_004174"
                     /pseudo
     CDS             complement(1..242)
                     /locus_tag="OO564_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            complement(244..>517)
                     /locus_tag="OO564_004175"
     CDS             complement(244..>517)
                     /locus_tag="OO564_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655044.1"
                     /translation="YLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYK
                     TDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
BASE COUNT          140 a          141 c          122 g          114 t
ORIGIN      
        1 cctggactcg ctgaagccca ggtaattaaa atcgtccttt accgtcagcc ggcgactgac
       61 acccacaatc tttccccaga catcaagacc atacttttct gcggtatcga tgttccagat
      121 aagaccataa aaatcactga taaaactgtc ggggaaagcg ctgcgttaaa gctgttaatg
      181 agggcattga gtcgggggct ggcagcatac tgcgcaagca cagtggcagc cacattctgc
      241 acgttatgcc tcctggagtt tcacactgat atccgacaca tccagaaccg gaatctcatc
      301 tatcccgaaa gtgacagccg ttgtccatga cgaaccgtca cggctcacag taaggcccag
      361 aatatcaata tgttccggat cggttttgta aatgccggca tagtagcgcc cggcagagac
      421 aacagaggca actcttgccc gcagaccacc atctgtacca ttaaatgcgt ccagcacgga
      481 ttgctgtacc tgtcgggtaa tatctgaagg caggtag
//
LOCUS       DAJTYI010000353          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYI010000353 DAJTYI010000000
VERSION     DAJTYI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>510
                     /locus_tag="OO564_004176"
     CDS             <1..>510
                     /locus_tag="OO564_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6655045.1"
                     /translation="YTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVFVLALLG
                     WIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSK
                     VKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLAN
                     VSGAPLMLTA"
BASE COUNT          121 a          108 c          124 g          157 t
ORIGIN      
        1 ttacaccatg tatccaccag aaattaaaaa ggtggataac aaaaccatcg ctaaagcggg
       61 ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct
      121 ggcgctactg ggctggattt tcagtaagtc tctgggggtt gatgaatcca ctgtggcaat
      181 cgttgttatg gcgaccatgc tgctgctggg tatcgttacc tgggaagacg tggttaaaaa
      241 taaaggcggc tggaatacct taatctggta cggcggtatt atcggcttaa gctccttatt
      301 atcgaaagtt aaatttttcg aatggttagc tgaagtcttt aaaaataacc tggcatttga
      361 tggtcacggt aacgttgctt tcttcgttat tattttcctc agcatcatcg tgcgttattt
      421 cttcgcttcc ggtagtgcct atatcgttgc tatgttacct gtatttgcca tgctggcgaa
      481 cgtctccggc gcaccgttaa tgttaaccgc
//
LOCUS       DAJTYI010000354          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYI010000354 DAJTYI010000000
VERSION     DAJTYI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>508
                     /locus_tag="OO564_004177"
     CDS             <1..>508
                     /locus_tag="OO564_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6655046.1"
                     /translation="WMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYW
                     HVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETL
                     TQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQ
                     MVKQFLSR"
BASE COUNT          128 a          113 c          175 g           92 t
ORIGIN      
        1 ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg ctggcgatgc
       61 cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg ctgaaagccg
      121 gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg aatacggcgc
      181 gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg gcacattaca
      241 acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa acggcgacgg
      301 aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg gagaagtttt
      361 ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag aagctgccgc
      421 aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg tgctatgcac
      481 aaatggtgaa acagttcctg agtcgtga
//
LOCUS       DAJTYI010000355          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYI010000355 DAJTYI010000000
VERSION     DAJTYI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>505)
                     /gene="queE"
                     /locus_tag="OO564_004178"
     CDS             complement(<1..>505)
                     /gene="queE"
                     /locus_tag="OO564_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6655047.1"
                     /translation="LEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVV
                     ITGGEPCIHDLLPLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVL
                     SQALERANEIKHPVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCI
                     ARNWRLS"
BASE COUNT          112 a          150 c          115 g          128 t
ORIGIN      
        1 atcgacaaac gccaattacg cgcaatacag gtttcaatgc acaatcgtgt ggcatcatcc
       61 ttttggctaa tcggctgcag tgcaatgact cgcggtttat catcggtcag cgttgccagt
      121 agttcatcca gtgcttcaat atcgcgtacg cgccccactg gatgcttgat ttcgttggct
      181 cgctccagtg cctgtgacaa cacttcatag ccgccgcgca tgttcagctt tggcgatacg
      241 gtaacccagg tattcggtgt gcagcgtact tcatgagttc cactggtttc gatctggcag
      301 ctaaaaccgt tcttttcgag caggtcagtc agtggcagca aatcatgaat gcaaggctca
      361 ccacccgtaa tcaccacatg ccgcgcggta tatccctgac gaccaatgac agccagcaag
      421 tcttcactgc tcgccgcccc ccacttatca ctctctttgg tcttcgccag aatgctgaaa
      481 agggagactt cccgatcctc aagct
//
LOCUS       DAJTYI010000356          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYI010000356 DAJTYI010000000
VERSION     DAJTYI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..419)
                     /locus_tag="OO564_004179"
     CDS             complement(<1..419)
                     /locus_tag="OO564_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HCR6655048.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAEL"
BASE COUNT          106 a          117 c          131 g          150 t
ORIGIN      
        1 cagagttcag cgggggaggg gaagtcatcc agcgttattt gcttttcaag gcattcacct
       61 tcggctgcgg tggcatcgcg gaagataaag ccatcaccgt tggtggcaaa aacaaaagga
      121 acgtccagca ggcgcgcata ttcaatgccc tgttgcatcc ctttgccaat ttcatgtttg
      181 ttggcttttg cttcaatcac ggctaaggga atgcctggct tgtggtacag cacgatgtca
      241 gcagatttta ccgttctgcg tgccgcaact ttaccacgta cgatgacttt accgtcccgg
      301 agtttgacct cctgtctgat ttgcgttgtg ttgtcccagc ctgcattgag aatggctggc
      361 ataacgcatt tggtaatgat gtctgcttcc gtcaggttac ttaggttcag ttccgccatg
      421 gggtgttctc gcttttatca ggacgattct gattgtacat aaaatcaata gcctggctat
      481 gatttagggc ggtgtttcaa cgta
//
LOCUS       DAJTYI010000357          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYI010000357 DAJTYI010000000
VERSION     DAJTYI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>503)
                     /gene="ipaH3"
                     /locus_tag="OO564_004180"
     CDS             complement(<1..>503)
                     /gene="ipaH3"
                     /locus_tag="OO564_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6655049.1"
                     /translation="QITVLEITQNALISLPELPASLEYLDACDNHLSTLPELPASLKH
                     LDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESL
                     EALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITHIPENILSLDPTCTIILEDN
                     PLSSRIR"
BASE COUNT          130 a           86 c          136 g          151 t
ORIGIN      
        1 ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc ggatcaaggc
       61 taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa aatatctcgg
      121 tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga agattagtac
      181 ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc tggttatttc
      241 ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc tgattgttat
      301 ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt tggttgttat
      361 ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg tgattgtcac
      421 aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg gcattctgag
      481 taatttccag aacagtgatt tga
//
LOCUS       DAJTYI010000358          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYI010000358 DAJTYI010000000
VERSION     DAJTYI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>498
                     /locus_tag="OO564_004181"
     CDS             <1..>498
                     /locus_tag="OO564_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6655050.1"
                     /translation="QASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLA
                     SLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLR
                     KQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGL
                     APVTR"
BASE COUNT           95 a          172 c          134 g           97 t
ORIGIN      
        1 cgcaggccag caaccgtatc cgcggccttc tgacccagat acatccggca ctggagcgcg
       61 ttctcggtcc gagacttgag cacccggcgg tactcgatct tctccagcga tatccctcac
      121 cagaaaaact cgcttcgctg ggtgagaaga agctggcagc ccagctctgc aaacttgcgc
      181 ctcgtctggg taaacgcctt gcagcagaca tagctcaggc actggccgaa caaaccgtcg
      241 tcgttcccgg cacgaatgcc gctgccgtag tactgccacg tctggcactc cagctcatca
      301 cgctgcgtaa gcaaagagac gaggtggcgc ttgaggtaga acagcgagtt cttgctcacc
      361 ctctttaccc ggtcctgacc agtatgcccg gagtcggtgt caggaccgca gccagactcc
      421 tcaccgaggt cgcctgccgc gccttcgcct ctgccgcaca tctcgctgct tatgctggcc
      481 ttgcgccggt aactcggc
//
LOCUS       DAJTYI010000359          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYI010000359 DAJTYI010000000
VERSION     DAJTYI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..339
                     /locus_tag="OO564_004182"
     CDS             <1..339
                     /locus_tag="OO564_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6655051.1"
                     /translation="DRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQGQSVTLTREGIVV
                     DGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYNGHRHRENGQGSN
                     TDKPDKAMEA"
     gene            339..>498
                     /locus_tag="OO564_004183"
     CDS             339..>498
                     /locus_tag="OO564_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6655052.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWP"
BASE COUNT          109 a          127 c          169 g           93 t
ORIGIN      
        1 gaccgttctc atgcggtggt tgttacggtg tcagaccggc gctaccgcct gaaagggctg
       61 cagacgggtg aggtggctgt ctatgacgat caggggcagt ccgtgacgct gacccgggag
      121 gggatcgtgg tggacggtgc aggtaaaacg atcacgtttc gcaatgcacc tgaagcacgt
      181 tttgaaatgg acctggaagt aacaggacag gtgaaagacc tgtgcgactc cggcggcacc
      241 accatgtcag caatgcggct tgcctataac ggtcatcgtc acagagagaa cggtcagggc
      301 agtaacaccg ataaacctga taaagctatg gaggcatgat ggaactgtgg ctgacggtga
      361 acggtaaacg cacctgcgcc agcgcaccgc tggatccgct gacccgcgcc gtggtgattt
      421 ccctgtttac ctggcggcgg gcggagcctg atgacaacgc cgacgtcccg atgggatggt
      481 ggggggatac ctggcctg
//
LOCUS       DAJTYI010000360          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYI010000360 DAJTYI010000000
VERSION     DAJTYI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.365"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>495
                     /locus_tag="OO564_004184"
     CDS             <1..>495
                     /locus_tag="OO564_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR6655053.1"
                     /translation="RETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYISS
                     NTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYT
                     NASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGI
                     AGDQ"
BASE COUNT          122 a          122 c          134 g          117 t
ORIGIN      
        1 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
       61 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
      121 attatatcag cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga
      181 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt
      241 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
      301 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
      361 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
      421 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
      481 tcgccggtga ccagc
//
LOCUS       DAJTYI010000361          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYI010000361 DAJTYI010000000
VERSION     DAJTYI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>485
                     /gene="ycjM"
                     /locus_tag="OO564_004185"
     CDS             <1..>485
                     /gene="ycjM"
                     /locus_tag="OO564_004185"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6655054.1"
                     /translation="GECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDPKTD
                     LSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCYLEK
                     GAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPHKDN
                     IA"
BASE COUNT          129 a          104 c          111 g          141 t
ORIGIN      
        1 ggtgaatgca gtcatttaat gtttgatttt gtctgcaacc atatgtctgc aaaaagtgaa
       61 tggtttaaaa actatttaca acagcttccg ggttttgaag atttctttat tgcggttgat
      121 ccgaaaaccg atctcagtgc cgtcacccgt ccgcgtgcgt taccgctatt aacgccgttt
      181 caaatggatg ataattccac gcgccattta tggaccacct ttagtgacga tcaaattgac
      241 ctgaattacc gtagccctga agtgttgctg gcgatggtgg atgttttact ctgttacctt
      301 gagaaaggtg ctgagtatgt ccgcctggat gccgttggct ttatgtggaa agagccggga
      361 acaagctgca tccatctgga aaaaacacat ctgattatca agctgttacg gtcgattatt
      421 gatgacgtag caccaggtac agtgatcatt accgaaacca atgttccgca caaagacaac
      481 attgc
//
LOCUS       DAJTYI010000362          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYI010000362 DAJTYI010000000
VERSION     DAJTYI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..261)
                     /locus_tag="OO564_004186"
     CDS             complement(<1..261)
                     /locus_tag="OO564_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR6655055.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAV"
     gene            complement(283..>484)
                     /locus_tag="OO564_004187"
     CDS             complement(283..>484)
                     /locus_tag="OO564_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6655056.1"
                     /translation="QQITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSN
                     GLLVGILLAVLMDNLIPWERIE"
BASE COUNT          137 a          129 c          101 g          117 t
ORIGIN      
        1 caccgctgtt ttcccgtctc cggtaaggcc ataaaaagca ttcacttttt cgcccatcgg
       61 cccgcagact tcaagcgtca cgatgttgcc acccgtcgcg gcgaccatcg catccaccgt
      121 cccttcgccg ccatccgcta tcggcaaaca gatatagtgc gcatcgggaa agagggtcga
      181 aaacccggct ttaattgcct gacaacattt ttctgcactt aagctctcct taaaagagtc
      241 tggcgcaatg acaatcttca ttatcaatcc tttttcaaca aattattcga tgcgttccca
      301 cggaataaga ttatccataa gaacagccag taaaattccg accagtaagc cgttactgag
      361 cagaggacga agcgttaatg gaaggtcttg caaatacaca ggcggtaatg ccattaaaaa
      421 tatgccgaca aataacggca atgcgagtcg ataaatattg cgctcggtaa acgttatttg
      481 ctgg
//
LOCUS       DAJTYI010000363          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYI010000363 DAJTYI010000000
VERSION     DAJTYI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            116..223
                     /locus_tag="OO564_004188"
     CDS             116..223
                     /locus_tag="OO564_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6655057.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT          137 a          116 c          123 g           99 t
ORIGIN      
        1 agagaaaacc cccgcacgtt gagaggttta acctgacaac agaacggggg ctaatcttga
       61 accagaacaa ttcaagaata gccgcaaaat gttgtcatta caacaaggcg gctatatgac
      121 gctcgcgcag tttgccatga ctttctggca cgacctggca gcaccgatcc tggcggggat
      181 tattaccgca gcgattgtca gctggtggcg taaccggaag taaggtgaaa acgggcgtca
      241 ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag gggtaacagc ctgcggccat
      301 ttttgtcttt atctcttcca caagtaaata ttggcaaccc gagctggaag catcatccag
      361 agcactcatg aaacgactgt aacagctgtt gaaaaaccgg aatgcactct tcattgcaaa
      421 catttcaggg atgaagtgaa gatgaattga gacgtagtat ccacatcaag ccggc
//
LOCUS       DAJTYI010000364          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYI010000364 DAJTYI010000000
VERSION     DAJTYI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.369"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(9..>470)
                     /locus_tag="OO564_004189"
     CDS             complement(9..>470)
                     /locus_tag="OO564_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6655058.1"
                     /translation="FSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTNSYLPR
                     LVEAQSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALR
                     DAPLVTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNR"
BASE COUNT           99 a          143 c          136 g           92 t
ORIGIN      
        1 tgtgccacct agcggttggt catttgagag catgaaccgg agagcactac gccgcgccca
       61 gcgagcggac gccctgcttc gcgagcctgg ttaccgtttt cttgcgccca ctgccgggcc
      121 aggccaatcg ccagaccaga accgcccgtt accagtgggg catcgcgcaa ggcttctccc
      181 tgaatttcca gatggtgttc ggtcagcgca tcaagcaccg cgtagcggta gccctcttgc
      241 tgtaagcgag ccagctcttg acgaacggca tccacacctt gttcgaaaac atgtgccgaa
      301 acgacgccgc agcgccctgt ggattgcgct tcaaccagac ggggaagata gctgttggtc
      361 atgggattta ccgggtgatg gcgcatcccg gattcggcca gcagttgatt cattacgaac
      421 aaatacccct gataaaccgt acgtccgttg accggcaggg ccggagagaa
//
LOCUS       DAJTYI010000365          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYI010000365 DAJTYI010000000
VERSION     DAJTYI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>464
                     /locus_tag="OO564_004190"
     CDS             <1..>464
                     /locus_tag="OO564_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6655059.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTYI010000366          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYI010000366 DAJTYI010000000
VERSION     DAJTYI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(332..>462)
                     /gene="rpsT"
                     /locus_tag="OO564_004191"
     CDS             complement(332..>462)
                     /gene="rpsT"
                     /locus_tag="OO564_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6655060.1"
                     /translation="AAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT          125 a           86 c          107 g          144 t
ORIGIN      
        1 caaggtctaa tgaggagata tttatgaatg gttaaagtat ataggaattt aattcaaaaa
       61 tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccatatt ctttgtgggg
      121 gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg ttctacgatt
      181 cttaagccac gaagagttca gatagtacaa cggcatgtct cttttgacta tctggcaacc
      241 ggcagtgtgt tctctcacgc atcacaaaag cagcaggcat aaaaaaaccc gcttgcgcgg
      301 gctttttcac aaagcttcag caaattggcg attaagccag tttgttgatc tgtgcagtca
      361 ggttagcctt atgacgtgca gctttgtttt tgtggatcag acctttagca gcctgacggt
      421 ccacgatcgg ttgcatttcg ttaaatgctt tctgtgcagc ag
//
LOCUS       DAJTYI010000367          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYI010000367 DAJTYI010000000
VERSION     DAJTYI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..165)
                     /locus_tag="OO564_004192"
     CDS             complement(<1..165)
                     /locus_tag="OO564_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655061.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEY"
     gene            complement(173..>452)
                     /locus_tag="OO564_004193"
     CDS             complement(173..>452)
                     /locus_tag="OO564_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6655062.1"
                     /translation="LSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNR
                     IPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT          129 a          110 c           84 g          129 t
ORIGIN      
        1 atattcagca atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata
       61 cacccgttcc tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa
      121 aatcagcaga ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt
      181 ttactaattt tccactttta tcccaggcgg agaatggcat gggtttctgg tacagatacc
      241 cctgagcctg aacgcatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc
      301 cttccgcaat caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt
      361 ttcccgcagt accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat
      421 taatttcata aaaacgtttc aggtttgaca aa
//
LOCUS       DAJTYI010000368          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYI010000368 DAJTYI010000000
VERSION     DAJTYI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..194)
                     /locus_tag="OO564_004194"
     CDS             complement(<1..194)
                     /locus_tag="OO564_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6655063.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASAR"
BASE COUNT          111 a          109 c          123 g          107 t
ORIGIN      
        1 aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct ctggccaagt
       61 tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat ccatactgca
      121 tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt ttccagttga
      181 aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt cggtgggcac
      241 acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac gggaaaaatt
      301 gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg agttgatcct
      361 ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg tcctcttaga
      421 gtgatcgtgc gcgcatgtta cgccagcaaa
//
LOCUS       DAJTYI010000369          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYI010000369 DAJTYI010000000
VERSION     DAJTYI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..63
                     /locus_tag="OO564_004195"
     CDS             <1..63
                     /locus_tag="OO564_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HCR6655064.1"
                     /translation="RRAKQYLNDMTDDDFNDFKE"
     gene            66..>444
                     /gene="yafO"
                     /locus_tag="OO564_004196"
     CDS             66..>444
                     /gene="yafO"
                     /locus_tag="OO564_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6655065.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAE"
BASE COUNT          134 a           89 c           99 g          122 t
ORIGIN      
        1 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
       61 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
      121 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
      181 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
      241 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
      301 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
      361 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
      421 ataaaattgg aaaaatggca gaag
//
LOCUS       DAJTYI010000370          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYI010000370 DAJTYI010000000
VERSION     DAJTYI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..74)
                     /locus_tag="OO564_004197"
     CDS             complement(<1..74)
                     /locus_tag="OO564_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6655066.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLI"
     gene            92..244
                     /locus_tag="OO564_004198"
     CDS             92..244
                     /locus_tag="OO564_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655067.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT          137 a           70 c           91 g          142 t
ORIGIN      
        1 ctgatgagca gtacgatacg ggtcatgcga ctgttaaatc ggtgtcgttt tcgcatacta
       61 gttgcctgac tcacaaaagg ttccttgaag tatgtcccac gctgtggacg gtacttacat
      121 taaggcacaa caggacaaaa tggtcaattc ttcgttatgt aaaaaagcgt cagtggatac
      181 atattttaat gttatggaag ttaatttaat tatttacaat gatgatgtaa taatgatgaa
      241 ttaacatgat aaacgtaata attcattaat cagggttatt ttgtttgata tatatcaatt
      301 ggaactttca tatgacactt agaatcaatg ctctctatag tgatgaataa tcatcattcg
      361 aagtcaggtg ggatgcctgt ctgaatacac ctccttcagg atgtggggga ttctgctgag
      421 catctatgaa actgaatgca
//
LOCUS       DAJTYI010000371          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYI010000371 DAJTYI010000000
VERSION     DAJTYI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..305)
                     /locus_tag="OO564_004199"
     CDS             complement(<1..305)
                     /locus_tag="OO564_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6655068.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEV"
     gene            complement(302..>439)
                     /locus_tag="OO564_004200"
     CDS             complement(302..>439)
                     /locus_tag="OO564_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6655069.1"
                     /translation="ILSAIVGWAADKCSLPPIVTLITFSAISLVCGFVYSKFIVFRDA
                     K"
BASE COUNT          139 a           96 c           88 g          116 t
ORIGIN      
        1 ataacctcaa tagggtcttg caggtcgaca tcaataggaa ttacagcatc gccgcttgca
       61 tggtctaacc ctgcaaataa ggctggttct ttaccaaagt tgcgtgtaaa tgacagcgga
      121 acaactagcg ggtctgaaac agccagcgcg ttaataattg actctgtggc atctttactt
      181 ccgtcattta tgaatataat ttctacttca tatggcttca actcttggaa ttcacgtacc
      241 gtcttataga aaacaggtat cgcttcttct tcattgaaga caggaacgac aagagatatt
      301 ttcatttcgc atccctaaaa acaatgaact ttgaatagac gaaaccgcac accagactga
      361 tggcggagaa ggtgataaga gtaactattg gaggaagtga gcatttatca gcagcccaac
      421 caacaatcgc actcaatat
//
LOCUS       DAJTYI010000372          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYI010000372 DAJTYI010000000
VERSION     DAJTYI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>438
                     /locus_tag="OO564_004201"
     CDS             <1..>438
                     /locus_tag="OO564_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6655070.1"
                     /translation="NGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLL
                     RLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTI
                     ELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWM"
BASE COUNT           95 a          116 c          121 g          106 t
ORIGIN      
        1 caatggctac gtcactcagg tactgatccg cccaggtaca tacgcagctg ccttgccgct
       61 gcgtccggtg atggtcttca tccccgagca aaaacggcaa attgtcgccc aatttcggca
      121 aaactcgctg ttacgtctga aacctggcga tgatgcggaa gtggtgttta acgcgctacc
      181 tgggcaggtg ttccacggca aattgactag tattttacct gtcgtgccag gcggttctta
      241 tcaggcgcag ggggtattgc aatcattaac ggtcgttccc ggcacggacg gtgtgctggg
      301 aaccattgaa ctggacccta acgatgatat cgatgcctta cccgacggca tctacgccca
      361 ggtggcggtt tactccgacc atttcagcca tgtttcggtg atgcggaaag tgctgctaag
      421 aatgaccagt tggatgca
//
LOCUS       DAJTYI010000373          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYI010000373 DAJTYI010000000
VERSION     DAJTYI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..217)
                     /locus_tag="OO564_004202"
     CDS             complement(<1..217)
                     /locus_tag="OO564_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6655071.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHW"
BASE COUNT          143 a           99 c           82 g          110 t
ORIGIN      
        1 cccaatgttg ttccagtaac tgcagacaac gtaaactttg cggcccacga tcgataacgt
       61 ctcccactga gataagtaaa tctcgccacg gatcaaaacg acaatgccat aatttgcggc
      121 gcaactgctc aagacaaccg tgtatatcgc cagaaagcca gatatgtcgc cattgatgac
      181 ccgcaattct ctgataaacg ggcgcaggct gtttcatcaa tattttcctc ccgcgctaaa
      241 gatcacataa tcttaacaag aatgttaaaa aacgctggac tcagacagta gagtgtgtgt
      301 tatggttgac tataaagtca gcgaaggaaa tgcttctggc ttttaacaga taaaaagaga
      361 ccgaacatga ttcctgtttt cgtcaacaaa caataaaacc ctttaaaatt aatgcgttga
      421 ataaatttac gttc
//
LOCUS       DAJTYI010000374          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYI010000374 DAJTYI010000000
VERSION     DAJTYI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..205
                     /locus_tag="OO564_004203"
     CDS             <1..205
                     /locus_tag="OO564_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655072.1"
                     /translation="FKNLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQIL
                     QYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          125 a           76 c           87 g          143 t
ORIGIN      
        1 gtttaaaaat ctcgagaggg aggtgttcat gacgcatggg tatgttgata gtcatattat
       61 tgatcaggct ttacgcttgc gtttaaaaga tgaaaccagt gtgattcttt ctgatctcta
      121 tctgcaaata ttgcagtaca ttgaaatgca taagactaca ctaacggata tcattattaa
      181 tgacagggaa tccgtgctct cttagaactt gagtaaggtt ttagaataaa catcagctgt
      241 atcaccatgc tgatgcaaag tgagcatttc aggcgttatg ctttcttatt atgtccgcaa
      301 tatcaggtgt caagaatgga gagttctcgc tctccattct tgacgcctga tatcccgcct
      361 aacttatttt gtactgccta cattatgctc aataattaat gggttagatc aagatggaga
      421 aaattttatc t
//
LOCUS       DAJTYI010000375          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYI010000375 DAJTYI010000000
VERSION     DAJTYI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.380"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(263..>429)
                     /locus_tag="OO564_004204"
     CDS             complement(263..>429)
                     /locus_tag="OO564_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6655073.1"
                     /translation="LKPFIKSESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFI
                     NFYMRYKPQH"
BASE COUNT          145 a           79 c           86 g          119 t
ORIGIN      
        1 cgaatataat tgcccatata ccattccgta aatagactcg tcatacttca agttgcatgt
       61 gctgcgactt gaattattga gagtaacaat taaaaaatca ggaaatggca gacgaatcca
      121 gggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
      181 gtcatgattt acctccttgc agtttgtgaa ataagaaaac gcgcacatac taggcgggcg
      241 ttattttcat tgcaagctgg atttaatgtt gcggtttata acgcatataa aaattaataa
      301 accacgctcc ccggcattca acgcgctgct gcttaactgt ctgaaaaaca taacgttcaa
      361 aaaagggttt tgcggttatg cttgcgtcca ccgtaagttc ggattcagac ttaataaaag
      421 gttttaaca
//
LOCUS       DAJTYI010000376          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYI010000376 DAJTYI010000000
VERSION     DAJTYI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(56..>429)
                     /locus_tag="OO564_004205"
     CDS             complement(56..>429)
                     /locus_tag="OO564_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6655074.1"
                     /translation="CIPAGTQTLRQRQQRLDAKVNLAGGINEDFYLAQLAALGRPDAT
                     ITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGDPCDSALRIWGDTV
                     VECVLNKLCPSHTYVIFKYPE"
BASE COUNT           91 a          113 c          121 g          104 t
ORIGIN      
        1 tacccgcgcc gaacttatcc ttctgcgcgg ttttcgtgtc tatacgatgc atggattact
       61 ccggatattt aaaaattacg taggtatgcg acgggcagag tttgttaagc acacactcga
      121 caacggtgtc accccagata cgcagcgcgg aatcacaggg atcgccacat gtcatccagg
      181 tggtgttggt ggcgactggc atgttgacct gccagtaata ccgccattcc ggcgcattca
      241 ccgcgtcagt acaggcagat gagcaggtga acgtgctttt gtcgtatcgc gtgatggtgg
      301 cgtctggtct gcccagggca gcaagctgtg caaggtaaaa atcctcattg atgccgcccg
      361 ccaggttaac cttcgcatcc agtcgttgct gacgctggcg aagggtctgt gtccctgcgg
      421 gaatacatt
//
LOCUS       DAJTYI010000377          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYI010000377 DAJTYI010000000
VERSION     DAJTYI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..344
                     /gene="mcbR"
                     /locus_tag="OO564_004206"
     CDS             <1..344
                     /gene="mcbR"
                     /locus_tag="OO564_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6655075.1"
                     /translation="IAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILC
                     EMIEQLWIRMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQ
                     NIAILYQQYNR"
BASE COUNT          145 a           88 c           85 g          105 t
ORIGIN      
        1 acatagcaga acttcaggaa ttgctcggga aattacagca ggcgcaagaa aagggcgaca
       61 tggaacaaat cattaatgcg aacaggctat ttcgcttagc gatttatcat cgctcaaata
      121 tgcccatcct gtgtgagatg attgagcaac tgtggatcag gatgggacct gctttacatt
      181 atctttatga agcgattaac ccagcggaat tacgggagcg catagaaaac tatcatctat
      241 tactcgctgc gttaaaagca aaagacaaag agggatgcaa acattgtctt gctgaaatta
      301 tgcaacaaaa tattgctatt ttataccagc aatacaatcg ttaaattaaa tgcccggcaa
      361 ataccgggca tcaagtaatt actcaaatcg ccacgcaata ttcatgccga caccataatt
      421 tcg
//
LOCUS       DAJTYI010000378          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYI010000378 DAJTYI010000000
VERSION     DAJTYI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     misc_feature    complement(17..140)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(116..166)
                     /gene="hisL"
                     /locus_tag="OO564_004207"
     CDS             complement(116..166)
                     /gene="hisL"
                     /locus_tag="OO564_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6655076.1"
                     /translation="MTRVQFKHHHHHHHPD"
BASE COUNT          121 a           79 c           93 g          120 t
ORIGIN      
        1 cgttaacaac cgcgcaataa aaaagccccc ggaaggtgat cttccggggg ctttctcatg
       61 cgttcatgca ccactggaag atctgaatgt cttccagcac acatcgcctg aaagactagt
      121 caggatgatg gtgatgatgg tggtgtttaa attgaacgcg tgtcataaaa gtctctgtga
      181 atgtttattc aactgatgtc ttttaaccta aaccactttc acgttagaaa gcaagggctt
      241 ttttatatat tttatggcga tggatttgtt taatgaatga gcggggaaag gagggggcta
      301 agaggactgt cctctaactt gacagcacca aaaagagcaa agtctgattt ttcgtctcgc
      361 tggcaatgtc ttgatctaca aactaattag taaatagtta attaacacct atc
//
LOCUS       DAJTYI010000379          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYI010000379 DAJTYI010000000
VERSION     DAJTYI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..405)
                     /locus_tag="OO564_004208"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="OO564_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
BASE COUNT           72 a          115 c           97 g          121 t
ORIGIN      
        1 aatgttttct gtggcgtaca gggccgtgcc acgaaaacct gtttcaatcc cgctgacgat
       61 tttctccagc tcaggccgga actgtgcttc agcctctcgg tgcaggctgg tcacggtcga
      121 ttcgatttgc gtcatcagtt caagggcttc cctgcgatca ctgaatttta tttcggggct
      181 gtccggatca aagtcccgca tcctgtcatg ctgatttccg gttccgaaaa tatcgtgaaa
      241 gaaagcgaat gcgtttctct cccggggctg cgctgaattt tcttcctcca gcatttgctg
      301 ttctttgcgt ttaccgcttc tgaaatagtc attcagctcc tcttccagtg aggtgcagac
      361 cccgtcagta attcccgggt tctggcatcg atctcctcca tggcc
//
LOCUS       DAJTYI010000380          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYI010000380 DAJTYI010000000
VERSION     DAJTYI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..129
                     /locus_tag="OO564_004209"
     CDS             <1..129
                     /locus_tag="OO564_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6655077.1"
                     /translation="LETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(175..>404)
                     /gene="rraB"
                     /locus_tag="OO564_004210"
     CDS             complement(175..>404)
                     /gene="rraB"
                     /locus_tag="OO564_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6655078.1"
                     /translation="EELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAEKFDVE
                     YDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
BASE COUNT           98 a          109 c           87 g          110 t
ORIGIN      
        1 ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt gggctttgag
       61 catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg gatgctacgc
      121 gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa ttaattagtg
      181 gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt cgccgttagg
      241 atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg ccagcgtcat
      301 cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc
      361 gcagcaaatc acgatatcac catcttcaac ttccagctct tctg
//
LOCUS       DAJTYI010000381          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYI010000381 DAJTYI010000000
VERSION     DAJTYI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            103..282
                     /locus_tag="OO564_004211"
                     /pseudo
     CDS             103..282
                     /locus_tag="OO564_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a          103 c          119 g           87 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgctgaactg
      121 ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa agtccatgcc
      181 gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg
      241 tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata agttatccat
      301 gctctgaggt gggtggaggt gggccctccg gtcagggttt acaggagcac tgaccaaacg
      361 acccggtgct gcttcattct aaggaatgag gtggtgagcg act
//
LOCUS       DAJTYI010000382          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYI010000382 DAJTYI010000000
VERSION     DAJTYI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..273
                     /gene="folK"
                     /locus_tag="OO564_004212"
     CDS             <1..273
                     /gene="folK"
                     /locus_tag="OO564_004212"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6655079.1"
                     /translation="LLNHTQRIELQQGRVRKAERWGPRTLDLDIMLFGNEVINTERLT
                     VPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
BASE COUNT          125 a           80 c           79 g          118 t
ORIGIN      
        1 ttactcaacc atacacagcg catagaactg caacagggtc gtgttcgtaa agctgaacgc
       61 tggggaccac gcacgctgga tctcgatatc atgctgtttg gtaatgaagt gataaatacc
      121 gaacgcctga ccgttccgca ctacgatatg aagaatcgtg gatttatgct gtggccgctg
      181 tttgagattg caccggagct ggcgtttcct gatggggaga cgttgcgtga agtcttacat
      241 acaagagcat ttgacaaatt aagcaaatgg taatttattt atattacgaa tataaatatt
      301 ttctgaaaat catacatcct ctctttcctt aataaaaatt ttagtttaaa catttttgca
      361 gaaacataat ataaattccc gcagataaat atcaatccca tc
//
LOCUS       DAJTYI010000383          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYI010000383 DAJTYI010000000
VERSION     DAJTYI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..132)
                     /locus_tag="OO564_004213"
     CDS             complement(<1..132)
                     /locus_tag="OO564_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="catalyzes the synthesis of pseudouridine from
                     U-2604 in the 23S ribosomal RNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6655080.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     "
BASE COUNT          119 a           89 c           89 g           96 t
ORIGIN      
        1 atcgccaatg gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc
       61 cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga
      121 gtcgggcagc ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa
      181 aacagggtta tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa
      241 gtgtctccgg aagtacgatg caaatttgcc tggcaaaata aggataatca acatattacg
      301 aatcgctgca cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc
      361 gctatttctc cccatgcaaa gagtgaaaaa tcg
//
LOCUS       DAJTYI010000384          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYI010000384 DAJTYI010000000
VERSION     DAJTYI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.389"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..321)
                     /locus_tag="OO564_004214"
     CDS             complement(<1..321)
                     /locus_tag="OO564_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6655081.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAK"
BASE COUNT           87 a          101 c           89 g          114 t
ORIGIN      
        1 ttttgcccgt ccatattcaa cggcttcttt gaggagttca ttttccatcg ttttcttgcc
       61 gagcaggcgc tggagttctt taatctgctt catggcggca gcaagttcag aggcaggaac
      121 aacctgttct ccggcggcca cagcagtaag acttccttcc tggtattgct tacgccagag
      181 aaataactgg ctggctgcta caccatgttg ccgggcaacg agggagaccg tcatccccgg
      241 ttcaaagctc tgctgaacaa ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc
      301 cggccctaag acatcaatca tctgttctcc aatgactagt ctaaaaacta gtattaagac
      361 tatcacttaa ataagtgata ctggttgtct g
//
LOCUS       DAJTYI010000385          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYI010000385 DAJTYI010000000
VERSION     DAJTYI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            56..>389
                     /locus_tag="OO564_004215"
     CDS             56..>389
                     /locus_tag="OO564_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655082.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQRK"
BASE COUNT          105 a           92 c           99 g           93 t
ORIGIN      
        1 tggtactcgt caaggtcgct aacaggataa aaaaatccca catccaggac ctataatggc
       61 gtatcggcta ctcattggta aaggaatgat caccttgaat ctcaaacgga tttttctcac
      121 cctcacctta cttcccttgt ttgctgttgc cgctgatgat tgcgcactct cagatccgac
      181 gctgaccgta caggcgtata ccgttaatcc tcaaacagag cgggtgaaaa tgtactggca
      241 aaaagccaat ggcgaagcgt ggggaacgtt acatgctctg ctggcggata ttaatagtca
      301 gggtcaggtg cagatggcga tgaacggcga catctatgat gaaagctatg cgccgctcgg
      361 tttgtacatc gaaaacggtc agcggaagg
//
LOCUS       DAJTYI010000386          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYI010000386 DAJTYI010000000
VERSION     DAJTYI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            52..>384
                     /locus_tag="OO564_004216"
     CDS             52..>384
                     /locus_tag="OO564_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6655083.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVW"
BASE COUNT           90 a           97 c          113 g           84 t
ORIGIN      
        1 tgccatcccc gccgggtgtt tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc
       61 agtgataaac cccgccagct ggcggtcccc tttgcgagta ccggagataa aaaccgtatc
      121 ccggacaagg cgacacagca gaccagagag agcggtaatg ctgcgtatga ttcaggtttt
      181 cctccggtga ccatgacagc ggtctcagcg ggaggtatac cgccacacgg caaggatttt
      241 aacggtctga tgtacgatat taccgcagca atacggttcg cccaggctgg cggtttgtac
      301 acgtataatg ccggttttgc gggggccatt ggtggatatg caaaaggagc cattctcgcc
      361 ggagtcgcaa caacagcggt ctgg
//
LOCUS       DAJTYI010000387          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYI010000387 DAJTYI010000000
VERSION     DAJTYI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>382
                     /locus_tag="OO564_004217"
     CDS             <1..>382
                     /locus_tag="OO564_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6655084.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLA"
BASE COUNT          102 a           79 c           95 g          106 t
ORIGIN      
        1 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
       61 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
      121 tttagaaaat catccggtca ttaccgggca tgaatgtgcc gggattattg tcgaagtggg
      181 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
      241 gttaccaagc ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta
      301 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
      361 ttttgctgcg gcgtcgctgg ca
//
LOCUS       DAJTYI010000388          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYI010000388 DAJTYI010000000
VERSION     DAJTYI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>381
                     /locus_tag="OO564_004218"
     CDS             <1..>381
                     /locus_tag="OO564_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6655085.1"
                     /translation="TEEGWFDDHQLRVADGVIAAIEPIPVGVTERDAELLCPAYIDTH
                     VHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAALKRIAQRCQRG
                     GPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT           85 a          103 c          117 g           76 t
ORIGIN      
        1 gactgaagag ggatggttcg atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc
       61 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta
      121 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
      181 actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt taccgaccac
      241 cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca
      301 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
      361 gcagaataaa ggcgcgcatc c
//
LOCUS       DAJTYI010000389          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYI010000389 DAJTYI010000000
VERSION     DAJTYI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>380
                     /gene="ybcL"
                     /locus_tag="OO564_004219"
     CDS             <1..>380
                     /gene="ybcL"
                     /locus_tag="OO564_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6655086.1"
                     /translation="KTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVTVYD
                     PDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGACPP
                     KGDKPHHYQFKVWALKTDKIPVDS"
BASE COUNT          104 a           78 c           92 g          106 t
ORIGIN      
        1 taaaaacagg agagcaactt acaacgtctc atgtattttc tggatttggg tgtgaaggtg
       61 gtaatacatc gccctcatta acctggtctg gtgctcctga aggcaccaaa agctttgccg
      121 taactgtgta tgatccagat gctcctacag gcagtggatg gtggcactgg actgttgcta
      181 atattccagc aactgtaaca tatttgccca ctgatgcagg aagacgtgat ggtacaaaac
      241 ttccgactgg tgctgttcaa ggccgaaatg attttggtta tgcaggattt ggtggagcat
      301 gtcctcccaa aggagataaa ccccatcatt accagtttaa agtatgggct ctaaaaactg
      361 ataagattcc tgtggattct
//
LOCUS       DAJTYI010000390          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYI010000390 DAJTYI010000000
VERSION     DAJTYI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>380)
                     /locus_tag="OO564_004220"
     CDS             complement(<1..>380)
                     /locus_tag="OO564_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6655087.1"
                     /translation="NVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPL"
BASE COUNT           87 a          119 c           72 g          102 t
ORIGIN      
        1 ttcagcgggt caaccttcag taacctctga tacatccctc tccaggtgat ttgcatcgcc
       61 gtttttctca ctgtctccgt tatgatgtac accacttctt ccagtaaccg ccgtttcgcc
      121 ggactcaaaa aaccgtagta cctcaccata cggaacccct tatccgccac atgccaggag
      181 aacctttcca tgaactcatc tccactcatc aacaggtatt cttcccgttt tgttcggtga
      241 ctgttgtaac gcagaccgat ttcatcctga ccggcataat gctccagacg actcatcggc
      301 accggtggct ttttcaggta agagccaaag tacaccgcca catgggtggc attatccatc
      361 acccgggata cgttgacatt
//
LOCUS       DAJTYI010000391          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYI010000391 DAJTYI010000000
VERSION     DAJTYI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..110)
                     /locus_tag="OO564_004221"
     CDS             complement(<1..110)
                     /locus_tag="OO564_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6655088.1"
                     /translation="MGSTRVVYLSNNADKSISVFSKEEKIPYLIQAWVDP"
     gene            complement(278..>378)
                     /locus_tag="OO564_004222"
     CDS             complement(278..>378)
                     /locus_tag="OO564_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6655089.1"
                     /translation="EMKFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          122 a           78 c           66 g          112 t
ORIGIN      
        1 aatggatcta cccaagcctg gatgagataa ggtatttttt cttctttgct aaatacagaa
       61 atagatttat cagcattatt acttaagtaa accacccgcg tactaccaat gacaacgcct
      121 gcataagtta atgctggttg gcctaaaaaa acgctgagta taatcgcggt aaataactta
      181 cattaaactg ttgttttaaa gagcctgatt aaacaacttt ttctggacat aacgactccc
      241 tggccaaagc caattgaata aaaaggccct tgataaatta tttcttatat tcgacagtaa
      301 attgtgaatc agcgttcgcc gtaccagccg ttacggtcgc attggttgcc acatagcgtg
      361 caataaactt catttcag
//
LOCUS       DAJTYI010000392          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYI010000392 DAJTYI010000000
VERSION     DAJTYI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>378)
                     /locus_tag="OO564_004223"
     CDS             complement(<1..>378)
                     /locus_tag="OO564_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6655090.1"
                     /translation="LVIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVP
                     LSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWA
                     VHFFLEYKTVSFIALGAVVLSITG"
BASE COUNT          119 a          103 c           81 g           75 t
ORIGIN      
        1 cccgtaatcg acagcaccac tgcccctaat gcaataaaag aaaccgtttt gtattcaagg
       61 aagaaatgca ccgcccacat cggattcagc gcatgcagca cttccgggtt agcaataatg
      121 ctacgtaacc ccagtcctgc cagaatcaaa aaccaggtca gcatgatcgg cgcaaacagc
      181 ttaccgacca tagccgtgcc atgcttttga atcataaata ataaagtgag aacgataatt
      241 gagaggggaa ctatccaggt atccagctgc ggggcgacga tttccagacc ttcaatggct
      301 gacatcaccg aaatagcggg tgttatgacg acttcaccat agaaaaagct gccgccgatt
      361 agccccataa tcaccagc
//
LOCUS       DAJTYI010000393          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYI010000393 DAJTYI010000000
VERSION     DAJTYI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.398"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..129)
                     /locus_tag="OO564_004224"
     CDS             complement(<1..129)
                     /locus_tag="OO564_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="positive regulator of the beta-glucoside operon;
                     transcriptional antiterminator; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6655091.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINS"
BASE COUNT          117 a           70 c           64 g          127 t
ORIGIN      
        1 tgagttaatt ctttcgccag cgcgtttttg aaagccaatt ccgcgcccca tgacgacttt
       61 ttcccgctgt tgatcatcaa taaccaccac aacattattg ttgagaattt tggtgatttg
      121 catgttcata gcaaggacct tttttataaa caaaaaaacc cgacttcacc agtattctct
      181 ggttatgtca ggttttgcct gcgaatgcag taacaatcca gtcatttatt aatggttttt
      241 ataacgaaca tccaggttcg gaaattaatt taattgcgtg cagagaaaat agcaatgcgc
      301 tattgataaa aatatgacca tgctcgcagt tattaacttt gtgtaatttt aggaatttat
      361 aaagttatat ataacaaa
//
LOCUS       DAJTYI010000394          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYI010000394 DAJTYI010000000
VERSION     DAJTYI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.399"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            1..374
                     /locus_tag="OO564_004225"
                     /pseudo
     CDS             1..374
                     /locus_tag="OO564_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           98 c           98 g           84 t
ORIGIN      
        1 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
       61 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      121 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      181 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      241 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      301 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      361 gtgcggagta aaca
//
LOCUS       DAJTYI010000395          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYI010000395 DAJTYI010000000
VERSION     DAJTYI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(13..>373)
                     /locus_tag="OO564_004226"
     CDS             complement(13..>373)
                     /locus_tag="OO564_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6655092.1"
                     /translation="DKVGIVRLDAFKASKDIQFAVQSGAMLMENGVINPRIHPNVASR
                     KIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGTISHMYMKGGAI
                     PWQRYPFVTMISVERKG"
BASE COUNT          110 a           94 c           70 g           99 t
ORIGIN      
        1 aaagctatca ttctaacctt tccgctcgac ggaaatcatg gtgacaaaag gatagcgttg
       61 ccacgggatt gccccgcctt tcatatacat atgtgaaatc gtgccaccaa gataaagcag
      121 ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat ttgtcgcctg
      181 ctggctcaac aaaaacacgg cattcccatg tttattaatc ccaacaccgt tacgaatttt
      241 acgtgaggcg acgttgggat gaatacgcgg attaattaca ccgttttcca tcaacattgc
      301 tcctgactgc accgcaaact gaatatcttt actggcttta aaagcatcca gacgaacgat
      361 gccgacttta tct
//
LOCUS       DAJTYI010000396          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYI010000396 DAJTYI010000000
VERSION     DAJTYI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..141
                     /locus_tag="OO564_004227"
     CDS             <1..141
                     /locus_tag="OO564_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655093.1"
                     /translation="DLPADALRTLALLQAELPLKKAAALAAEIHGVKKNALYKYALEQ
                     QG"
     gene            complement(184..>371)
                     /locus_tag="OO564_004228"
     CDS             complement(184..>371)
                     /locus_tag="OO564_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6655094.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
BASE COUNT           96 a           99 c          101 g           75 t
ORIGIN      
        1 gacttacccg ccgatgccct gcgcacgctg gcgctgctac aggcagaact gccgctgaaa
       61 aaagcggcgg cgctggccgc agaaattcat ggcgtgaaga aaaatgcgct gtataagtat
      121 gcgctggagc agcaggggta acagtgccga agcgttgcgt taatcaaaac gcaacgtctt
      181 cttttaccgc accatgacgg taatggtggc tgtgccctga aattttcctg tttccagctc
      241 accaccgacc agattaactg gccaggcgcg catattgacg gtgccatgcc cggaaggatc
      301 aacgggaagg ataccgttct gaaaaggaat atttgcaact ccgccgccga gattttcaat
      361 acgaataccg a
//
LOCUS       DAJTYI010000397          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYI010000397 DAJTYI010000000
VERSION     DAJTYI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.402"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>366)
                     /locus_tag="OO564_004229"
     CDS             complement(<1..>366)
                     /locus_tag="OO564_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR6655095.1"
                     /translation="GAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLLTVSLVIIAL
                     ADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVIGDVLNNPDL
                     APLIKTLSLAFVVIPHGQQF"
BASE COUNT           97 a          110 c           91 g           68 t
ORIGIN      
        1 gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc
       61 caggtccggg ttattcagca cgtcgccgat gacatcactc aacaaaaaca ccgacacaca
      121 taccacaagc cccagcccga cgttcagcca gtacagcgtg gtgagttcaa ggtgactgat
      181 ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat ctgccagcgc
      241 gataatcacc agcgacacag tcagcaggcc gaactggtgg ttgtcgataa tccgcgccag
      301 cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtggcaatag ccgaccactt
      361 cgcgcc
//
LOCUS       DAJTYI010000398          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYI010000398 DAJTYI010000000
VERSION     DAJTYI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.403"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..157)
                     /locus_tag="OO564_004230"
     CDS             complement(<1..157)
                     /locus_tag="OO564_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6655096.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLT"
BASE COUNT          111 a           93 c           63 g           98 t
ORIGIN      
        1 gcgtcagaag ccgcaatccc aatcgttctt ctatacggcg cactatctgg cttaaagctg
       61 actgcaccat gctcaggcgg gctgctgcgc gagtgaagct acgctcctct gcaacgacga
      121 caaacgccat cagatcagcg atttcttcac gcttcattca gtatctccat acaatctatt
      181 aatcatttaa atgataggtc taatcatgat ttagggtcta atcaacttaa tgatcaatat
      241 ctatagtgat atcaccaacc gtcaaaacga accgacaact ccatgaggaa ctgaatgatg
      301 aataataaag tcagcttcac taacagcaat aatccaacca tctctttgtc tgcagtgatc
      361 tattt
//
LOCUS       DAJTYI010000399          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYI010000399 DAJTYI010000000
VERSION     DAJTYI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.404"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..309)
                     /gene="bamD"
                     /locus_tag="OO564_004231"
     CDS             complement(<1..309)
                     /gene="bamD"
                     /locus_tag="OO564_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6655097.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     P"
BASE COUNT           81 a           89 c           90 g          101 t
ORIGIN      
        1 cggatgggtc gggttaaggc gaataaaacg atcgatggca gcctgcgcta acggcaaatc
       61 ggcgttttta tagtaggcgt agatgagatc cagctgcacc tgctgcgaat acggaccaaa
      121 cggatagcga ttatctaacg cttccagttg cgttattgcc tgtctccagt taccgtcctg
      181 cagcttttgt tgtgcagtcg cgtaaatttc atttggcgga ttatcaggta cttcttcctt
      241 tgaccccgag caacccgcca aaaacaggct tagtgtggcg gctgccacca gatatttcat
      301 gcgcgtcatg acgttttgac tttcctcaaa atgtaatacg ggagattctc tgttcctgct
      361 c
//
LOCUS       DAJTYI010000400          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTYI010000400 DAJTYI010000000
VERSION     DAJTYI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.405"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(1..360)
                     /locus_tag="OO564_004232"
                     /pseudo
     CDS             complement(1..360)
                     /locus_tag="OO564_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          105 a           94 c           93 g           68 t
ORIGIN      
        1 cgacggtaac cgtcagaatt gggaaggaag caataatcag tacgttcgtg cacagtgatg
       61 cctaggtaaa tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg
      121 taacgaagaa gttgataccg gtaagcgtcg tacctatacc ggatagctgg agactccata
      181 tccagtaatc aacaccgact cccggactgt actctattcc cgatagcggt ggataggcca
      241 gccagccggt ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga atcacgccaa
      301 caacagtaaa ccagaagctt aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga
//
LOCUS       DAJTYI010000401          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTYI010000401 DAJTYI010000000
VERSION     DAJTYI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.406"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT           97 a           82 c           94 g           83 t
ORIGIN      
        1 atacactcag tggcgtaatc gttgacaata tggtgcatgt aatcgtaagc gtccagcgaa
       61 atccatctgg tcataatctg aagcatccga caaagtggtg tccaccaaat aagtgatctg
      121 cgcccaatat cctgagccgg aactttgaca aaggccaacg aaaaatggga gcggatgtta
      181 cagagttctc ggtacagggg aaaagtggta cctgtcgcca gttctccgat ctgtttaacc
      241 gggaaattat ctcctacagc ttgtcagaaa ggtcggtgat ggagcatcgt taatacgatg
      301 cattcacagt gcccggccag aggatgcccc gtcgctgcat atggatcagg gttggc
//
LOCUS       DAJTYI010000402          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTYI010000402 DAJTYI010000000
VERSION     DAJTYI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.407"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..195
                     /locus_tag="OO564_004233"
     CDS             <1..195
                     /locus_tag="OO564_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6655098.1"
                     /translation="MLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLSENGSQLHHS"
BASE COUNT           83 a          103 c          102 g           67 t
ORIGIN      
        1 atgctggaga atgagtaccc tcagagggtg gctgaccggc tgaaagcatc aggtctgagc
       61 ggtgatgcgg atgcggagag ggaagccggt gcacaggtga tgcgtgagac tgaacagcag
      121 atttaccgtc agctgactga cgaggtactg gccctgcgat tgtctgaaaa cggctcacaa
      181 ctgcaccatt cataatcaca tcgcataaac cacagaccgg actgactccg gaaaaacgga
      241 ggcccgcccc cgggcctccc ctgattcatc cgtttccctg ttcagcctga cagcacgccc
      301 cggctgccgg atgacagact ccgcttcggt aagcaaagcg gtcttctgtg attcc
//
LOCUS       DAJTYI010000403          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTYI010000403 DAJTYI010000000
VERSION     DAJTYI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.408"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..351)
                     /gene="cybB"
                     /locus_tag="OO564_004234"
     CDS             complement(<1..351)
                     /gene="cybB"
                     /locus_tag="OO564_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6655099.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGL"
BASE COUNT          115 a           89 c           75 g           73 t
ORIGIN      
        1 caaaccaaac gcaaaccacg ggttgccccg gttatacatc atcaccaaac caatcactgg
       61 cagcgcaata aacaggagat aaatcaccaa atgtcccaaa tgcgccagtc ccgtcatcat
      121 cggtttcggc ttaggtataa tcggcggggt tgggtatttc agtcttaaca aaagacgaac
      181 caccatcagc acgagaattg agatgccaca ggaaacatga atcatgttga tgagcggccg
      241 atcgctacgt gggaagaaac cacgaaactc cattgcgcaa tacgctgcga taaccagcaa
      301 aaagaccagc cagtgaatgc tgatttgtaa ccttgagtat ttattttcca ta
//
LOCUS       DAJTYI010000404          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTYI010000404 DAJTYI010000000
VERSION     DAJTYI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.409"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            85..>351
                     /locus_tag="OO564_004235"
     CDS             85..>351
                     /locus_tag="OO564_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6655100.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDA"
BASE COUNT           73 a          101 c          105 g           72 t
ORIGIN      
        1 cggggaatac agccatcagt tgcttgcacc ggtggacaat atctccattg cgaaaaacga
       61 actggcggta ctggggacga tttcatggac gtgacaaacg atgattacat ccgcctgtta
      121 tcggcactgt tgccgcccgg tccggcgtgg tcatctagcg atccggcgat tgccggtgcg
      181 gcaccgtcat taacccgtgt tcatcagcgt gcggatgccc tgatgcggga gctggatccg
      241 cgcaccacca ctgaactgat aaaccgctgg gagcgtctgt gcggtctgcc ggatgaatgt
      301 attcccgcag ggacacagac ccttcgccag cgtcagcaac gactggatgc g
//
LOCUS       DAJTYI010000405          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTYI010000405 DAJTYI010000000
VERSION     DAJTYI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.410"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            3..293
                     /gene="ubiK"
                     /locus_tag="OO564_004236"
     CDS             3..293
                     /gene="ubiK"
                     /locus_tag="OO564_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6655101.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT          115 a           83 c           76 g           76 t
ORIGIN      
        1 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta
       61 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
      121 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
      181 tacggactcg tgagaaactg gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca
      241 gtactgagat caaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
      301 tctttaatgc actgtaaatt atgaattaat cctctaattt acagtgtttt
//
LOCUS       DAJTYI010000406          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTYI010000406 DAJTYI010000000
VERSION     DAJTYI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.411"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..152
                     /locus_tag="OO564_004237"
     CDS             <1..152
                     /locus_tag="OO564_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6655102.1"
                     /translation="RGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIESKHRPKYIIK
                     NEKQK"
BASE COUNT          110 a           62 c           66 g          110 t
ORIGIN      
        1 ttcgcggata tccctccctg cttgtatcaa tacttttctt gggtggagtg cagctgatcg
       61 ggattggtgt tctcggtgaa tatataggca gaatctatat tgaaagcaaa catcgtccca
      121 aatacatcat caaaaatgag aaacaaaaat gaaaagcctt aaaacatcat atgtaaaaaa
      181 actatttttc ttaattgctg tatttttcat tgcggttatt ttgatgtatt tgcgccgccc
      241 tgatataatc agaacaccgc agttttgggc agaagatggt catgtatggt atgctatggc
      301 atacaataat ggcatattca catcaatgat attccctcaa aatggata
//
LOCUS       DAJTYI010000407          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTYI010000407 DAJTYI010000000
VERSION     DAJTYI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.412"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>347)
                     /locus_tag="OO564_004238"
     CDS             complement(<1..>347)
                     /locus_tag="OO564_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6655103.1"
                     /translation="IFNSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRT
                     GRISMGHGLVVTGDIFTNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHT
                     DRKFVIGGLGNRQ"
BASE COUNT           97 a           87 c           69 g           94 t
ORIGIN      
        1 attgcctatt ccccagccca ccaattacga atttacggtc ggtatggtcc tggcgaagca
       61 atgcctgcgc accatcgttg gataccgcat tacggcccat aataacgttc tggtcacgca
      121 tatgaatcca catgcctgta ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg
      181 taacaaccag accatgcccc atgcttatcc ggccagttct gagattaagc gtaaaggggc
      241 gtagtggccc tatattacca ttttcccctt cattctctcg tgtagggatg atatgcaggc
      301 attcttcaga acgacgaaaa atggcaccaa aagatgaatt aaatatt
//
LOCUS       DAJTYI010000408          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTYI010000408 DAJTYI010000000
VERSION     DAJTYI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.413"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>345)
                     /locus_tag="OO564_004239"
     CDS             complement(<1..>345)
                     /locus_tag="OO564_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6655104.1"
                     /translation="YRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGVY
                     DTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSYQRL
                     ITHFYNHLIFALP"
BASE COUNT          110 a           70 c           75 g           90 t
ORIGIN      
        1 gggcaacgca aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc
       61 aacaatggct gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct
      121 ttgtacggtt aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt
      181 gcttaacgag aaaaaggttt tcgtcaccgt atcatacaca ccccattgca atgtcgggtc
      241 atcagacatc acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg
      301 catataggtc attttatagc cagagaaaaa aggcgtatcg cggta
//
LOCUS       DAJTYI010000409          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTYI010000409 DAJTYI010000000
VERSION     DAJTYI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.414"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..280
                     /locus_tag="OO564_004240"
     CDS             <1..280
                     /locus_tag="OO564_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6655105.1"
                     /translation="ANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASF
                     SLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT           97 a           71 c           84 g           91 t
ORIGIN      
        1 tgcaaattat aaagaaaacc aaatagcggg aatgaagcca ggaatgaagg ctgagatccg
       61 tgctgatatt ctcaaagggg aagtgtttca cggtcacatt gaaagccttt ccccagcgac
      121 aggagccagt ttcagcttga ttccgataga aaatgccact ggcaatttca ccaaaattgt
      181 tcagcgtgtc ccggtacgga ttgcatttga cgacgcaaag gaattaaagc aacttcttcg
      241 cccgggactg tccgtcaccg tcagtgttga tgagagatga tgtctatgcg taaacatatt
      301 gctttcgcca gtatgtgtat gggattattt attgctcagc ttg
//
LOCUS       DAJTYI010000410          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTYI010000410 DAJTYI010000000
VERSION     DAJTYI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.415"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT          117 a           40 c           58 g          126 t
ORIGIN      
        1 ccttatttcc tccgaccagc gatcaatgcc ggtagagttg tccattgtat tattggtgag
       61 ctatgttgct ttacattaat gacagtgatg tgtgtcgatt aaatattgtt ttaagcattc
      121 atcataaaaa tgtatcattc gtttcaaaag atgtctgtgt ttgtatgaat atattatttt
      181 tcgtggattc taatgatgat tattaataaa tgtcaaatat gaaagaaacc atgtctggga
      241 aaaattttac agataattga ttttgtgaat atgatgatta aaatttcaca attaaatccg
      301 aaaaaaagag acgtaaatgc ttgttaatta acaaatgata t
//
LOCUS       DAJTYI010000411          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTYI010000411 DAJTYI010000000
VERSION     DAJTYI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.416"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>341
                     /locus_tag="OO564_004241"
     CDS             <1..>341
                     /locus_tag="OO564_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR6655106.1"
                     /translation="GTMKAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPV
                     LHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGE
                     EGIFTGLASLN"
BASE COUNT           95 a          102 c           83 g           61 t
ORIGIN      
        1 cggcacgatg aaagcaggca aatcaaccac catcaacgcc attgtggggc aggaaattct
       61 gcctaaccgt aaccgcccca tgacctctgt accgacgctc atccgccacg ttcccggaaa
      121 aactgagccg gttctccatc tggaacatat tcagcctgtc cgcaatttat taatcacact
      181 gcaggaaaaa ctcgccaccc cggcaggaca gcaggtcgca cagaccctgc agcaaaccgg
      241 ggatacccgc gaactgctgg atattctgac ggatgatggc tggctcaaaa atgaatacca
      301 cggggaggag ggaatcttta ccggactggc atcgttaaac g
//
LOCUS       DAJTYI010000412          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTYI010000412 DAJTYI010000000
VERSION     DAJTYI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.417"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            65..>339
                     /locus_tag="OO564_004242"
     CDS             65..>339
                     /locus_tag="OO564_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6655107.1"
                     /translation="MRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWK"
BASE COUNT          131 a           66 c           88 g           54 t
ORIGIN      
        1 tgattcgaaa tggattcccg gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa
       61 tgaagtgcga cagactgaag ataaacagca ccagaacagc gaaccggagg agggaacacc
      121 ctgcaatcag actggcgagg ataactgccc tgactgtggc gcggtgatgg gagacgcaac
      181 ttatcaggaa acttttgacg aaaaaaaacc agatgaagca caggaagaag agccgaagaa
      241 aacggaaaaa gctgatgatt tgttaccgga gaacgctggc agcgatcagc ataatgatag
      301 caataatgaa actggcgaag atgaactcaa ctggaaaaa
//
LOCUS       DAJTYI010000413          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTYI010000413 DAJTYI010000000
VERSION     DAJTYI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.418"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..297)
                     /locus_tag="OO564_004243"
     CDS             complement(<1..297)
                     /locus_tag="OO564_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6655108.1"
                     /translation="MRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAK
                     VNLAGGINEDFYLAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQV"
BASE COUNT           65 a          101 c          101 g           71 t
ORIGIN      
        1 gacctgccag taataccgcc attccggcgc attcaccgcg tcagtacagg ccgatgagca
       61 ggtgaacgtg cttttgtcgt atcgcgtgat ggtggcatct ggtctgccca gggcagcaag
      121 ctgtgcaaga taaaaatcct cgttgatgcc gcccgccagg ttaaccttcg catccagccg
      181 ttgctgacgc tggcgaaggg tctgcgttcc cgccggaata cattcatccg gcaggccgca
      241 cagacgctcc cagcgattta tcagttcggt ggtggtgcgc ggatccagct cccgcatcag
      301 ggcatccgca cgctgatgaa cgcgggttaa tgacggtg
//
LOCUS       DAJTYI010000414          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTYI010000414 DAJTYI010000000
VERSION     DAJTYI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.419"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            79..>337
                     /locus_tag="OO564_004244"
     CDS             79..>337
                     /locus_tag="OO564_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6655109.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANR"
BASE COUNT          111 a           67 c           81 g           78 t
ORIGIN      
        1 taacgttgac ggtgtgtatt accgaccact gcaaaaactg attaatggca cctggtataa
       61 cgtagcgagt atttaacaat gttgcattta aaaaatatta ctgcgggtaa tccgaaaaca
      121 gcagaacaat atcagatgac aaaacgatat tcggtcacct ggcttttttc agaagacgga
      181 aaaaactggt atgaagagct gaagaatttc gccagcgaca caataaaaat agcttacacc
      241 ggagatggtc gcgtggtgtg ggtcggtaag gatgtgacag gcatcgagcc acgcaatgcc
      301 agcgttatcg aagttccgga tattaccgct aaccgac
//
LOCUS       DAJTYI010000415          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTYI010000415 DAJTYI010000000
VERSION     DAJTYI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.420"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>333)
                     /locus_tag="OO564_004245"
     CDS             complement(<1..>333)
                     /locus_tag="OO564_004245"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6655110.1"
                     /translation="KTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHIS
                     VQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPL
                     SHFDARRVD"
BASE COUNT           91 a           66 c           96 g           80 t
ORIGIN      
        1 atcgacgcgg cgcgcatcaa aatgcgatag cggggagaat tccgttggat gataaatgcc
       61 gtctgcagtt tcatcgccga tttgcgccag ttctgttgtc gggaagtagc gagttaaatc
      121 agcgctcatt gagcgatcaa gtgtcaattc agagccatcg atgacataag gataagggat
      181 ttcatgttgc gagggttgca cagagatatg cgcaccataa tcctgataaa gcaacgttag
      241 ttgctcattc agatacgaac gaaagagagt agggcgagta atcgtggtgg tgtagctgcc
      301 tgcgtgagta aaacgaccaa atgcgcgcgt ttt
//
LOCUS       DAJTYI010000416          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTYI010000416 DAJTYI010000000
VERSION     DAJTYI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.421"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT           83 a           69 c           87 g           94 t
ORIGIN      
        1 ttgcatgagc aagcggactg acacatttct ctttgttctc attcagaaaa tctcatcagt
       61 cggcgttcag accggtgttg tgctttatgg atacgttacg ctcctgatga cgtcatttga
      121 cgttcaacag catcacgggg ccgcacgaca tttcacgtca gttagtgcta tagctcagga
      181 acaaattttc ccaaattggg atatgctcgc aaattgctgg tgatgtgggg agaatctggt
      241 tgagttcggt agaattgatt tggaggcaga acgcttaaat cgtggcgtcc tgaaacgaaa
      301 aacggacctc cgtggaggtc cgttgatatg aat
//
LOCUS       DAJTYI010000417          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTYI010000417 DAJTYI010000000
VERSION     DAJTYI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.422"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT          118 a           55 c           65 g           91 t
ORIGIN      
        1 ggcgaagaga atctgacatt gatagagtcc attaacgtta taagaggact ctatcatcgc
       61 caatcatcag acattgaaat agacgacact gagatgacgc ttacaattct tttggtttct
      121 gtaataagta atctgacatt ccccgtgccc agaagaggag agggatatca agcccaaggg
      181 ttaataattg atactacggt taaaattgcc ttcagtaaag gaaacgtgat taaattcagg
      241 ggatttcaga gtgaaaacca tacaacatat taaaatctgt tcatttagct ataatacata
      301 caagaaagtg tccagttaga taatgttat
//
LOCUS       DAJTYI010000418          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTYI010000418 DAJTYI010000000
VERSION     DAJTYI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.423"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT          108 a           59 c           62 g          100 t
ORIGIN      
        1 gtttaagctc cattttcaaa ataatatatc agggtttgtt actaataatt tctggaaaca
       61 gaaaaagtag agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacat
      121 actgaacgtt atcgtgatat atttatcatg cgttcttcac cattattcga gtgcagggac
      181 gcaatgacct taaatacctt ttagtagaat atattgctga cccacaaata tcaggatggt
      241 attgacaagt catagatgga gaaatatatg actgaattac cattctctgg aataacagca
      301 gactccttcc catagacggc ttagggggg
//
LOCUS       DAJTYI010000419          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTYI010000419 DAJTYI010000000
VERSION     DAJTYI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.424"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            106..>322
                     /locus_tag="OO564_004246"
     CDS             106..>322
                     /locus_tag="OO564_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655111.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEG"
BASE COUNT           97 a           60 c           87 g           78 t
ORIGIN      
        1 ggaaggtatc ccggcaacag agatgttcaa cgtgattcca ctggcaccgg gccatgccta
       61 cgggatgttt atggaacgct ttaacgagtt atcggagtta cgcaaatgcg catgaatgtt
      121 ttcgaaatgg aagggtttct tcgtgggaga tgtgtaccgc gagatctgaa agtaaatgaa
      181 acagatgctg aatacctggt gcgtaaattc gatgcgcttg aagctaaatg tgcagcacag
      241 gaaaacaaag taataccagt gtcaactgaa ctgccaccag caaatgaaag tgttttgtta
      301 ttcgatgcta acggagaagg ct
//
LOCUS       DAJTYI010000420          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTYI010000420 DAJTYI010000000
VERSION     DAJTYI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.425"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>320)
                     /gene="ompG"
                     /locus_tag="OO564_004247"
     CDS             complement(<1..>320)
                     /gene="ompG"
                     /locus_tag="OO564_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6655112.1"
                     /translation="GLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPL
                     TLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFE
                     WQDH"
BASE COUNT           86 a           87 c           72 g           75 t
ORIGIN      
        1 cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga aagtccgttc tggaaatcat
       61 aaccgtaaaa taaacccaca cggttaaaat catggccttc acgttcaata tcatcctgcc
      121 agtcccagtt actccagcga tccagcccaa tgcgcgtata cggcgtaacc gagtggttgc
      181 cgagcgtcag cggcaaatag gcgcgaattt cttgtgtgga aaactcaccg ttattgcggc
      241 tgtcgtccat attgaagccg cgctcgagat aatagttcac tcgcaaagca accgtttcgt
      301 tgaaggtata ttgcagacct
//
LOCUS       DAJTYI010000421          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTYI010000421 DAJTYI010000000
VERSION     DAJTYI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.426"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..198
                     /locus_tag="OO564_004248"
     CDS             <1..198
                     /locus_tag="OO564_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6655113.1"
                     /translation="WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVW
                     FGEIGVHEFAYLVKNYVAAVN"
BASE COUNT           88 a           74 c           68 g           90 t
ORIGIN      
        1 tggctccagg gcgacggttg caaagccaat acctgcggct gggactttat cgtaacactc
       61 tataacccaa aaacccatga agtcgttggc taccgctact ttggtttaga tgacccggcc
      121 tacctggttt ggttcggcga aattggcgtg catgaattcg cgtatctggt gaaaaactac
      181 gtagctgcgg ttaactaatt taaggagtct gtcatgaaaa cttttatcaa aactttactc
      241 gttgctgtaa ctattctgtt ctctgtcttc gctacggcga aacaagtaaa actgccaaac
      301 aacatcaaat acgttaatac
//
LOCUS       DAJTYI010000422          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTYI010000422 DAJTYI010000000
VERSION     DAJTYI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.427"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT           88 a           66 c           63 g          103 t
ORIGIN      
        1 ttctccattc attgcctctt caaggcgttc cttgaaacgc attatttccg gggcataaaa
       61 atgcgctctg catttttgct ttctttttca cctgtcatgc tcaatctctc tgttatataa
      121 agaattttac agaattcttt gatttgcatt ggttaaaata aaaatgtgca tagctcttga
      181 aatgcgcatt tttctgcgat atcgtttttc ccgatagcgg tagccggtat cagttaccga
      241 aaacgttaag agagtaagga tcgcagaatg tcagcaaaaa accaagactg gcacgcagag
      301 cagataaagg ctgctgttcg
//
LOCUS       DAJTYI010000423          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTYI010000423 DAJTYI010000000
VERSION     DAJTYI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.428"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
BASE COUNT          111 a           69 c           54 g           85 t
ORIGIN      
        1 attatccgat taaggtgaag cagaaaaagc acgctaccgc cccaggctca acagctacct
       61 gaatactgat accgcactga tattgacgcg ctagtgtatc acttctttcg cggcaatccg
      121 acccgaatga tttttaagca cattgttgat tttcaataaa gaaatcaatc aaataaaatc
      181 attagcaacc atattctttc actgttgctc tgcgattcct tcctgcacgt acagcagaaa
      241 tcaatgaatc gcataaaaat acaaaaaaag tatagatgtt cagcaagaat tggcgggtaa
      301 attgctattt aagtcaaaa
//
LOCUS       DAJTYI010000424          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTYI010000424 DAJTYI010000000
VERSION     DAJTYI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.429"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>314
                     /locus_tag="OO564_004249"
     CDS             <1..>314
                     /locus_tag="OO564_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCR6655114.1"
                     /translation="VLGFILGLFISVSAVLARAMLRRGVEAPEQLEEHGISVYSTIPM
                     SEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPADSAVEAVRALRTSLHFAMMETE
                     NN"
BASE COUNT           70 a           75 c           85 g           84 t
ORIGIN      
        1 ggtgcttggt tttattcttg gcctgtttat ttcggtgagt gccgtgctgg cgcgtgcgat
       61 gttgcgtcgt ggtgtagaag ccccggaaca actggaagag cacggcatca gcgtttattc
      121 cactatccca atgtccgagt ggctggataa acgcacccgt ctgcgtaaga aaaatttatt
      181 ttctaatcag cagcgccatc gtactaaaaa tatccccttc ctggcggtgg ataacccggc
      241 ggattctgct gtggaagccg tacgtgcgct acgaaccagt ctgcatttcg ctatgatgga
      301 gactgagaat aaca
//
LOCUS       DAJTYI010000425          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTYI010000425 DAJTYI010000000
VERSION     DAJTYI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.430"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..87
                     /locus_tag="OO564_004250"
     CDS             <1..87
                     /locus_tag="OO564_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6655115.1"
                     /translation="TAGEPKAQLAREFNISRETLYQYLRTDD"
     gene            91..>313
                     /locus_tag="OO564_004251"
     CDS             91..>313
                     /locus_tag="OO564_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR6655116.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEP"
BASE COUNT           56 a          122 c           89 g           46 t
ORIGIN      
        1 acggccggcg agcccaaggc gcagcttgcc cgcgagttca acatcagccg ggaaaccctc
       61 taccagtacc tccgcacgga cgactgacac atgccgcgtc gcttgatcct ctcggccacg
      121 gagcgggaca ccctgcttgc gctgccggaa agccaggatg acctgatccg ctactacacc
      181 ttcaacgact ccgacctgtc gctgatccgc cagcgacgcg gcgacgccaa ccgcctcggc
      241 ttcgccgtgc agctctgcct gctgcgctac cccggttacg cgctgggaac cgacagcgag
      301 ctgcccgagc cgg
//
LOCUS       DAJTYI010000426          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTYI010000426 DAJTYI010000000
VERSION     DAJTYI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.431"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..161
                     /locus_tag="OO564_004252"
     CDS             <1..161
                     /locus_tag="OO564_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6655117.1"
                     /translation="TLSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNS
                     STAELLLK"
     gene            231..>311
                     /locus_tag="OO564_004253"
     CDS             231..>311
                     /locus_tag="OO564_004253"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Converts D-sorbitol-dphosphate to
                     D-fructose-6-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate 2-dehydrogenase"
                     /protein_id="HCR6655118.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVE"
BASE COUNT          102 a           54 c           69 g           86 t
ORIGIN      
        1 aaacgctctc tatcgaaatg aataaattaa agcaggcgcg atattccatt ggcattgcca
       61 tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat attaattgtc
      121 tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc cgatgtttac
      181 cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt atgcaaacgt
      241 ggttaaattt gcaggataaa atcattattg tcaccggcgg cgcatccggt attggtctgg
      301 cgattgtaga g
//
LOCUS       DAJTYI010000427          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTYI010000427 DAJTYI010000000
VERSION     DAJTYI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.432"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>309
                     /locus_tag="OO564_004254"
     CDS             <1..>309
                     /locus_tag="OO564_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655119.1"
                     /translation="QQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAE
                     RQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAE
                     A"
BASE COUNT           98 a           75 c           86 g           50 t
ORIGIN      
        1 caacaagaag cgtttatgac gcttgagcag gagcagcagg ttaaaacccg tactgccgaa
       61 cagaatgcac gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga
      121 attctggctg aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca
      181 agaaaggttg aggctgaacg tgaagttcgt attaaagaga tcgaacagca gcaggtcacc
      241 gaaatcgcca accagacgaa atcgatcgct attgccgcca aatcggaaca acagtcccag
      301 gcagaagcc
//
LOCUS       DAJTYI010000428          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTYI010000428 DAJTYI010000000
VERSION     DAJTYI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.433"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            105..>309
                     /gene="ompL"
                     /locus_tag="OO564_004255"
     CDS             105..>309
                     /gene="ompL"
                     /locus_tag="OO564_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6655120.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELK"
BASE COUNT           90 a           64 c           69 g           86 t
ORIGIN      
        1 ggcgacagaa aaaaaaacgg cttccgtaac gttataaatg tcaatagccg ctatttccat
       61 cctggtgggt ggcggcctcc ctacgtttaa aaaatggact tattatgaaa aagattaatg
      121 caataatttt attatcttct ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa
      181 acagggaggc atataatctt gcctccgatc agggcgaggt tatgctacgt gtgggttata
      241 acttcgatat gggcgcgggt attatgttaa ccaataccta cacctttcag cgagaagatg
      301 aactaaaac
//
LOCUS       DAJTYI010000429          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTYI010000429 DAJTYI010000000
VERSION     DAJTYI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.434"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>308)
                     /locus_tag="OO564_004256"
     CDS             complement(<1..>308)
                     /locus_tag="OO564_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR6655121.1"
                     /translation="PVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     "
BASE COUNT           66 a           94 c           80 g           68 t
ORIGIN      
        1 ggcgatactg ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc
       61 cggtcagcac attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc
      121 ccactttagt gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca
      181 tcactttcat ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag
      241 tctggcggaa aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca
      301 gcaccggg
//
LOCUS       DAJTYI010000430          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTYI010000430 DAJTYI010000000
VERSION     DAJTYI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.435"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>305
                     /locus_tag="OO564_004257"
     CDS             <1..>305
                     /locus_tag="OO564_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6655122.1"
                     /translation="AFLREMINGKQKTWLAHCTDAEALCIDRVISEVLAEHPGLISVL
                     RQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWLNLAEYMLYLPMRDAFSSGD"
BASE COUNT           73 a           57 c           94 g           81 t
ORIGIN      
        1 agcgtttttg cgagagatga tcaatggcaa gcaaaagacc tggctggcgc attgtactga
       61 tgcagaggcg ttatgtattg atagagtcat aagtgaggtg ctggcagagc atcctggatt
      121 gattagtgtc ctccggcaac ggtatgaagg gcgggggatg actaagcgta aaatggctga
      181 attgctgaat gacgcacacc ctgagtggtg tttccggacg tgctgcagtc gggtagatgt
      241 atggctaaat cttgctgaat atatgctcta tctaccgatg cgtgatgcat tctcttccgg
      301 ggatc
//
LOCUS       DAJTYI010000431          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTYI010000431 DAJTYI010000000
VERSION     DAJTYI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.436"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..>303)
                     /locus_tag="OO564_004258"
     CDS             complement(<1..>303)
                     /locus_tag="OO564_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655123.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTT"
BASE COUNT           62 a           84 c           84 g           73 t
ORIGIN      
        1 cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt ggacgttcag
       61 gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga
      121 gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg ttacgcccca
      181 gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg tagatcattt
      241 catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc gatgaggtta
      301 att
//
LOCUS       DAJTYI010000432          302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTYI010000432 DAJTYI010000000
VERSION     DAJTYI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.437"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..210
                     /locus_tag="OO564_004259"
     CDS             <1..210
                     /locus_tag="OO564_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655124.1"
                     /translation="NDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEG
                     VEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          108 a           58 c           69 g           67 t
ORIGIN      
        1 aacgacgcca aacgcgcaaa agctgatttg ctggaaaaaa cattcacctc ccgctcatgg
       61 gaaagaatca aaggtatgga ctggccgaca atcaaagctg gtcgtaatgc actatggatt
      121 gaactggaag gcgttgaata tgcattcccg gatccacaaa cacaaaacga aacgcaacaa
      181 gcaggatacg atgagaatat tccggtgtaa aaccaagtgc attttgaaca cggtttttga
      241 aaatattaat ggaggtgttc atgtataaaa ttacagccac tattgaaaag gaaggtggtt
      301 ct
//
LOCUS       DAJTYI010000433          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTYI010000433 DAJTYI010000000
VERSION     DAJTYI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.denovo.439"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..>300
                     /locus_tag="OO564_004260"
     CDS             <1..>300
                     /locus_tag="OO564_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6655125.1"
                     /translation="EWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIK
                     MKAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF"
BASE COUNT           98 a           63 c           72 g           67 t
ORIGIN      
        1 aggagtggcg acgctttacc gatcgatttg ttaacagtca ggcgcacatt attatgtgcg
       61 ggcgtgcagg atatgagtat gactttttcg aaggtgatga tggcaaacgc cagttggcaa
      121 aaaccggtat aaaaatgaaa gcagaaacag aaaccggtta tgaaccctcc atcctgatcc
      181 agatggagaa gcaaatgaac atagaaaccg gtcaggtgtg gcgcaccgcc agaatactaa
      241 aagatcgctc cacccgtatt gatggacagg tattttcaaa cccaacattt aaaaactttc
//
LOCUS       DAJTYI010000434         7845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.guided.159, whole genome shotgun sequence.
ACCESSION   DAJTYI010000434 DAJTYI010000000
VERSION     DAJTYI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.guided.159"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(49..1506)
                     /gene="pepD"
                     /locus_tag="OO564_004261"
     CDS             complement(49..1506)
                     /gene="pepD"
                     /locus_tag="OO564_004261"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6655126.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1768..2226
                     /gene="gpt"
                     /locus_tag="OO564_004262"
     CDS             1768..2226
                     /gene="gpt"
                     /locus_tag="OO564_004262"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6655127.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2318..3562
                     /gene="frsA"
                     /locus_tag="OO564_004263"
     CDS             2318..3562
                     /gene="frsA"
                     /locus_tag="OO564_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6655128.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3620..4021
                     /gene="crl"
                     /locus_tag="OO564_004264"
     CDS             3620..4021
                     /gene="crl"
                     /locus_tag="OO564_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6655129.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4060..5115)
                     /gene="phoE"
                     /locus_tag="OO564_004265"
     CDS             complement(4060..5115)
                     /gene="phoE"
                     /locus_tag="OO564_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6655130.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            5403..6506
                     /gene="proB"
                     /locus_tag="OO564_004266"
     CDS             5403..6506
                     /gene="proB"
                     /locus_tag="OO564_004266"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6655131.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6518..7771
                     /gene="proA"
                     /locus_tag="OO564_004267"
     CDS             6518..7771
                     /gene="proA"
                     /locus_tag="OO564_004267"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6655132.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         1900 a         1961 c         2040 g         1944 t
ORIGIN      
        1 gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg
       61 aatttctttc agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac
      121 ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca tgtccatttc
      181 cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg
      241 cgtcttgttg aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc
      301 gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc
      361 cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg aacggatcag
      421 gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc
      481 gaccacacct tttgccacgt cggaattgcg gatcacaccg ttcggggtgg cgttcagcag
      541 acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc
      601 cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata
      661 ggtattcacc agagatttca gtgcgtcgac tttatcagct gtgacagcaa tggtcgcaaa
      721 ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa ggcgcaggtc
      781 cagttcttct gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc
      841 aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt
      901 ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc
      961 ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc
     1021 agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggttat
     1081 ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc
     1141 agaggccata ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc
     1201 gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt
     1261 ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt ccatacctgc
     1321 ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt
     1381 ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg
     1441 aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc
     1501 agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag
     1561 cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tattgagaat accactttat
     1621 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     1681 agtgcgccag atatctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     1741 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     1801 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     1861 attattgccg taagccgtgg cggtctggta ccgggggcgt tactggcgcg tgaactgggt
     1921 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     1981 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     2041 gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     2101 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     2161 gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     2221 cgctaatctt ttcaacgcct ggcattgccg ggcgttgttc tttttaactt caggcaggtt
     2281 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     2341 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     2401 tcacggcgca caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc ctcactggta
     2461 tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt gacccacgtg aaatcctcga
     2521 cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt tatacgatac
     2581 cgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg cgatggtgtg
     2641 gcaacaaaaa gcctgtacgg aagaagatcc gcaactcagt ggtcgacact ggctgcatgc
     2701 ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc tggccgagca
     2761 agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc cgggcacgat
     2821 gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt tgcatatgcc
     2881 gcaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg cgatgcagac
     2941 ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga tgctgactat
     3001 tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact ccagcctgtt
     3061 gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca ctcgcgtcgc
     3121 ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc ttgaatcgcc
     3181 gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga gtgattttaa
     3241 gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt tggggatgca
     3301 tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa aagtgcaagg
     3361 attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga acgatccgtt
     3421 cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta aattattaga
     3481 gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa tcaccggctg
     3541 gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc caatttggta aaacagttgc
     3601 atcacaacag gagatagcaa tgacgttacc gagtggacat ccgaagagca gattgatcaa
     3661 aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata atcgattctt
     3721 tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc gtgaattctg
     3781 gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct accagtttgg
     3841 tctgttcgat aaagcaggcg actggaagag tgttccggta aaagatactg aagtggttga
     3901 acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc tgacgacgct
     3961 gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt taacggcgtg
     4021 agtgaaatgt gccggatgca tcacatccgg caatattcat taaaactgat acgtcatccc
     4081 aaccgcgaca gtatcatcat tattaatatt cagcttatta tcgctatcca gtttgttgat
     4141 tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac cgacgtcgat
     4201 ataattgacc agatcttcat caccgatgcc ctcaatatct ttccctttcg ataagacata
     4261 acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt caaagttctg
     4321 tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag aatagaaagt
     4381 tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt ctgcacgctt
     4441 gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg tataggcccc
     4501 actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga agccatcgcc
     4561 gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg ttaagttcag
     4621 gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc tggcgcgttt
     4681 ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga acatatcggt
     4741 ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag aacccaaatc
     4801 tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat cactctccgc
     4861 tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat cgttaatttg
     4921 agtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac tggcgttatc
     4981 actcatataa tgcatggctt taactttgcc atagacatcc agtttattac cgtctttatt
     5041 atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca ctaatgccag
     5101 agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc agcgggagag
     5161 ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt acagattaat
     5221 gatattctaa aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct
     5281 acgcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga
     5341 cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa
     5401 tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat
     5461 cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg
     5521 ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc
     5581 tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc
     5641 agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc
     5701 aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca
     5761 ccctgcgtgc gttgctcgat cacaatatcg ttccggtaat caatgagaac gatgctgtcg
     5821 ctacggcaga gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg
     5881 cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc
     5941 gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg
     6001 cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg
     6061 ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg
     6121 gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga
     6181 ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg
     6241 tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca
     6301 ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag
     6361 gtcgcgatat cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg
     6421 gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc
     6481 accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc
     6541 cgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct
     6601 ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca
     6661 ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact
     6721 gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga
     6781 tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg
     6841 tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga
     6901 tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac
     6961 ctgtcgcact aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt
     7021 accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct
     7081 gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact
     7141 gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta
     7201 cgttgatgaa agcgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca
     7261 gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag
     7321 cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc
     7381 agctgcgctg acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga
     7441 gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga
     7501 tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga
     7561 tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc
     7621 ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac
     7681 acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat
     7741 cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat
     7801 ttgattcaca aggccattga cgcatcgccc ggttagtttt aacct
//
LOCUS       DAJTYI010000435         5893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.guided.188, whole genome shotgun sequence.
ACCESSION   DAJTYI010000435 DAJTYI010000000
VERSION     DAJTYI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.guided.188"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..468
                     /locus_tag="OO564_004268"
     CDS             <1..468
                     /locus_tag="OO564_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HCR6655133.1"
                     /translation="NWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVR
                     ITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFL
                     KHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            461..1195
                     /gene="frdB"
                     /locus_tag="OO564_004269"
     CDS             461..1195
                     /gene="frdB"
                     /locus_tag="OO564_004269"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6655134.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1206..1601
                     /gene="frdC"
                     /locus_tag="OO564_004270"
     CDS             1206..1601
                     /gene="frdC"
                     /locus_tag="OO564_004270"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6655135.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1612..1971
                     /gene="frdD"
                     /locus_tag="OO564_004271"
     CDS             1612..1971
                     /gene="frdD"
                     /locus_tag="OO564_004271"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6655136.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2034..3167
                     /locus_tag="OO564_004272"
     CDS             2034..3167
                     /locus_tag="OO564_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6655137.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            3256..3789
                     /gene="blc"
                     /locus_tag="OO564_004273"
     CDS             3256..3789
                     /gene="blc"
                     /locus_tag="OO564_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6655138.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3786..4015)
                     /locus_tag="OO564_004274"
                     /pseudo
     CDS             complement(3786..4015)
                     /locus_tag="OO564_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(4191..4337)
                     /gene="ecnB"
                     /locus_tag="OO564_004275"
     CDS             complement(4191..4337)
                     /gene="ecnB"
                     /locus_tag="OO564_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6655139.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4448..4573)
                     /gene="ecnA"
                     /locus_tag="OO564_004276"
     CDS             complement(4448..4573)
                     /gene="ecnA"
                     /locus_tag="OO564_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6655140.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(4625..5191)
                     /gene="efp"
                     /locus_tag="OO564_004277"
     CDS             complement(4625..5191)
                     /gene="efp"
                     /locus_tag="OO564_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6655141.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5233..>5893
                     /locus_tag="OO564_004278"
     CDS             5233..>5893
                     /locus_tag="OO564_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR6655142.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QI"
BASE COUNT         1466 a         1533 c         1519 g         1375 t
ORIGIN      
        1 aactgggcga agatccgcga cgaaatgggc ctggcaatgg aagaaggttg cggtatctac
       61 cgtacgccgg aactgatgca gaaaaccatc gacaagctgg cagagctgca ggaacgcttc
      121 aagcgcgtgc gcatcaccga cacctccagc gtgttcaaca ccgacctgct ctacaccatt
      181 gaactgagcc acggtctgaa cgttgctgaa tgtatggcgc actccgcaat ggcacgtaaa
      241 gagtcccgcg gcgcacacca gcgtctggac gaaggttgca ccgagcgtga cgacgtcaac
      301 tttctcaaac acaccctcgc cttccgcgat gctgatggca cgactcgcct ggagtacagc
      361 gacgtgaaga ttactacgct gccgccagct aaacgcgttt acggtggcga agcggatgca
      421 gccgataagg cggaagcagc caataataag gagaaggcga atggctgaga tgaaaaacct
      481 gaaaattgag gtggtgcgct ataacccgga agtcgatacc gcaccgcata gcgcattcta
      541 tgaagtgcct tatgacgcaa ctacctcatt actggatgcg ctgggctaca tcaaagacaa
      601 cctggcaccg gacctgagtt accgctggtc ctgccgtatg gcgatttgtg gttcctgcgg
      661 catgatggtt aacaacgtgc caaaactggc atgtaaaacc ttcctgcgtg attacaccga
      721 cggtatgaag gttgaagcgt tagctaactt cccgattgaa cgcgatctgg tggtcgatat
      781 gacccacttc atcgaaagtc tggaagcgat caaaccgtac atcatcggca actcccgcac
      841 cgcggatcag ggtactaaca tccagacccc ggcgcagatg gcaaagtatc accagttctc
      901 cggttgcatc aactgtggtc tgtgctacgc cgcgtgcccg cagtttggcc tgaacccaga
      961 gttcatcggt ccggctgcca ttacgctggc gcatcgttat aacgaagata gccgcgacca
     1021 cggtaagaag gagcgtatgg cgcagttgaa cagccagaac ggtgtatgga gctgtacttt
     1081 cgtgggctat tgctccgaag tctgcccgaa acacgtcgat ccggctgcgg ccattcagca
     1141 gggcaaagta gaaagttcga aagactttct tatcgcgacc ctgaaaccac gctaaggagt
     1201 gcaacatgac gactaaacgt aaaccgtatg tacggccaat gacgtccacc tggtggaaaa
     1261 aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac ggcggttccg gctgtgtggt
     1321 tcagcattga actgattttc gggctgtttg ccctgaaaaa tggcccggaa gcctgggcgg
     1381 gattcgtcga ctttttacaa aacccggtta tcgtgatcat taacctgatc actctgacgg
     1441 cagctctgct gcacaccaaa acctggtttg aactggcacc gaaagcggcc aatatcattg
     1501 taaaagacga aaaaatggga ccagagccaa ttatcaaaag tctctgggcg gtaactgtgg
     1561 ttgccaccat cgtaatcctg tttgttgccc tgtactggta aggagcctga gatgattaat
     1621 ccaaatccaa agcgttctga cgaaccggta ttctggggcc tcttcggggc cggtggtatg
     1681 tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg gtattctgct gccactgggg
     1741 ctgtttccgg gtgatgcgct gagctacgag cgcgttctgg cgttcgcgca gagcttcatt
     1801 ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt ggtgtggttt acaccgtatg
     1861 caccacgcga tgcacgatct gaaaatccac gtgcctgcgg gcaaatgggt tttctacggt
     1921 ctggctgcta tcctgacagt tgtcacgctg attggtgtcg ttacaatcta acgcatcgcc
     1981 aatgtaaatc cggcccgcct atggcgggcc gttttgtatg gaaaccagac cttatgttca
     2041 aaacgacgct ctgcgcctta ttaattaccg cctcttgctc cacatttgct gcccctcaac
     2101 aaatcaacga tattgtgcat cgcacaatta ccccgcttat agagcaacaa aagatccccg
     2161 gtatggcggt ggcggtaatt tatcagggta aaccttatta ctttacttgg ggctatgcgg
     2221 acatcgccaa aaagcagccc gtcacacagc aaacgttgtt tgagttaggt tcggtcagca
     2281 aaacatttac gggcgtgctt ggtggcgacg ctattgctcg aggggaaatc aagttaagcg
     2341 atcccgcaac aaaatactgg cctgaactta ccgctaaaca gtggaatggg atcacactat
     2401 tacatcttgc gacctacacc gctggcggcc tgccattgca ggtgccggat gaagtgaaat
     2461 cctcaagcga cttgctgcgc ttctatcaaa actggcagcc tgcatgggcc ccaggaacac
     2521 aacgtctgta tgccaactcc agtatcggtt tgttcggcgc actggctgtg aagccgtctg
     2581 gtttgagttt tgagcaggcg atgcaaactc gtgtcttcca gccactcaaa ctcaaccata
     2641 cgtggattaa tgtacctccc ccagaagaaa agaattacgc ctggggatat cgcgaaggta
     2701 aggcagtgca tgtttcgcca ggggcgttag atgctgaagc ttatggtgtg aagtcgacca
     2761 ttgaagatat ggcccgctgg gtgcaaagca atttaccccc ccttgatatc aatgagaaaa
     2821 cgcttcaaca agggatacaa ctggcacaat ctcgctactg gcaaaccggc gatatgtatc
     2881 agggcctggg ctgggaaatg ctggactggc cggtcaatcc tgacagcatc attaacggca
     2941 gtgacaataa aattgcactg gcagcacgcc cggtaaaagc gattaagccc ccaactcctg
     3001 cagtacgcgc atcatgggta cataaaacgg gggcgaccgg cggatttggt agctatgtcg
     3061 cgtttattcc agaaaaagag ctgggtatcg tgatgctggc taacaaaaac tatcccaatc
     3121 cagagagagt cgccgccgcc tgtcagattc tcaacgctct acagtaaaat ttcatcgggt
     3181 ccgaattttc ggaccttttc tgcgcttttc cttgctgtca tctacactta gaaaaaaacc
     3241 agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg
     3301 gtcgttgcct gtagttctcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac
     3361 gccaaacgtt atctcggtac ctggtatgag attgcccgtt ttgatcaccg ttttgaacgt
     3421 ggactggaaa aagtcaccgc aacatacagc ctgcgtgatg acggcggcct gaatgtcatt
     3481 aataaaggct ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt
     3541 accggcgcac caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt
     3601 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc
     3661 gactacctgt ggatactctc ccgcacgcca accatttctg acgaagtgaa acaggagatg
     3721 ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc
     3781 ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc
     3841 gccagacgca tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg
     3901 actgcgccaa tacccgtcca cacggcatca aacggctaaa gccgtgggta tatttcaggc
     3961 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     4021 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     4081 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     4141 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     4201 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     4261 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     4321 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
     4381 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
     4441 agaaaaatta gctggcagcg cgagagatgg agttgccgag atgtttgatg tcttcgccga
     4501 aaccgcaagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
     4561 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
     4621 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
     4681 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
     4741 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
     4801 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
     4861 cctgatccag cagccattta gcgttgtcgc caattgcttt tgcatcagca gacagctgct
     4921 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
     4981 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
     5041 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
     5101 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
     5161 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
     5221 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
     5281 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
     5341 gcggacgaaa aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc
     5401 cgctcattta tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcgtcaggta
     5461 cttacctcgc aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc actggaagaa
     5521 cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
     5581 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
     5641 aatcagggca acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa
     5701 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac
     5761 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt
     5821 ctgccgattg tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct
     5881 acgctgcaaa tct
//
LOCUS       DAJTYI010000436         4204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.guided.208, whole genome shotgun sequence.
ACCESSION   DAJTYI010000436 DAJTYI010000000
VERSION     DAJTYI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.guided.208"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO564_004279"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO564_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6655143.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1451..2254
                     /locus_tag="OO564_004280"
                     /note="aph(3'')-Ib"
     CDS             1451..2254
                     /locus_tag="OO564_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6655144.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2254..2394
                     /locus_tag="OO564_004281"
                     /pseudo
     CDS             2254..2394
                     /locus_tag="OO564_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-kinase"
     gene            complement(2584..4161)
                     /locus_tag="OO564_004282"
     CDS             complement(2584..4161)
                     /locus_tag="OO564_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HCR6655145.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
BASE COUNT          948 a         1088 c         1056 g         1112 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa
     1441 ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc
     1501 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1561 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     1621 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     1681 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     1741 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     1801 tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     1861 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     1921 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga
     1981 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     2041 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     2101 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     2161 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     2221 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     2281 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     2341 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaaggggga
     2401 accgcagaat tcggaaaaaa tcgtacgcta agctaacggt gttctcgtga cagctctttg
     2461 actaggcttt ctaaggccat tctgatagcc ctgacttcct gaaaagccat ggctaaaatt
     2521 tgtgcggcta aaagggataa ccgatggtaa agtaagttat ccctgtcgag atactgaaaa
     2581 gcgttatcct cgtttttccc aaaactgttt tgccagttcg ctcagagcgc tagttaactg
     2641 agcgacagat ttcgcacttt gcaaattatt ctgcccggtc tgtacattgg tatcagcagc
     2701 atcgcgtata ttgataatac tgcggtttat gtcttcactt actgctccct gctgctcgac
     2761 cgcagtcgct atttgcgcgt tcatgtcggt aatttcgtta acgcgttggc caattccatc
     2821 aagagctgta gctgcttcct ctgcgtgagc tacactcgtg tgcgcttgcc gactactttg
     2881 ctccatgact gtaacagcgg attgcgctcg ctcttgtaga gcgctgatca tgctttgaat
     2941 atccgttgtc gattgctgtg tgcgagcagc aagactgcga acctcatcgg cgacaacagc
     3001 aaaaccacgc ccctgctcac cagcacgcgc ggcctcaatt gctgcgttga gtgccaacaa
     3061 attcgtttgc tcggcgatcc ctcgtataac gtcaagaact tttgatatct cgttactttg
     3121 accttcaagc tcatgaataa cctgagtggc ttgcctaatt tcaccttcaa gggcagtgat
     3181 tgactggctt gtgtgggcta ccagacgctg gccagatgcc gtctcagtat ctgctcttcc
     3241 ggccgcatct gcagcatgct gtgcattgct cgcaacctct tgaatgcttg ccaccatttg
     3301 gtttactgcc gttgctattt gatctgtctc tgcctgctgc tcaactgtaa gtacattgct
     3361 tgactcaata tcctttagta ggcctcgggt gtgttcgcta agccgatttg atgcatcacc
     3421 tatgcgacct actatggcgc ctgtttcagc ttgcatcatt cgtaaagcaa actctatttg
     3481 gccaaactca tcggtgcgcc cagtgtagag ggattgactt aatgggttat tggaaatatt
     3541 cctggctctt tcaaccagtc ttccaagagg agagagaata gccaaaacac taacagagct
     3601 taagcttcct gacattaaag tggctaacaa taagctgctt attgatgtat cagtaagcat
     3661 gccggcagcc attgcgcttg atataatact accccatatg agcaagagta ttttcacgga
     3721 aaagctagca gccaatttcg gcctcgcggc cttcccgctt ctcaattgag catataattt
     3781 ttccgcagcc aaaacctgct caggttcagg cttggtcctt acagactggt attcaacaat
     3841 cgaaccattc ttagctattg gcgttacata agcacttacc caatagtggt cgccattttt
     3901 acagcgattt tttactagcc ccatccatga gcggccagat tttaatgtac tccacatatg
     3961 ctcaaatgca gcaggcggca tatctgggtg tcttacgatg ttgtgaggct ggcctaatag
     4021 ttcttcctca gtgaaaccac tgattttaat gaagtcagga ttaacgtacg tgatatggct
     4081 ttgaggggag gtagtcgaaa gaatattggc atcttttggg agttctaagt ttcgacccgt
     4141 cactggtaaa ttctggcgca taagaacctc aagggttggc tgttttattt tattgttttc
     4201 ggca
//
LOCUS       DAJTYI010000437         2642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.guided.236, whole genome shotgun sequence.
ACCESSION   DAJTYI010000437 DAJTYI010000000
VERSION     DAJTYI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.guided.236"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            complement(<1..332)
                     /gene="tetR(A)"
                     /locus_tag="OO564_004283"
     CDS             complement(<1..332)
                     /gene="tetR(A)"
                     /locus_tag="OO564_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6655146.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQM"
     gene            438..1637
                     /gene="tet(A)"
                     /locus_tag="OO564_004284"
     CDS             438..1637
                     /gene="tet(A)"
                     /locus_tag="OO564_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6655147.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1669..2553)
                     /locus_tag="OO564_004285"
     CDS             complement(1669..2553)
                     /locus_tag="OO564_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR6655148.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
BASE COUNT          499 a          816 c          806 g          521 t
ORIGIN      
        1 tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg
       61 agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg
      121 gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc
      181 agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc
      241 gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca
      301 cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata
      361 atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc
      421 tggtccggag gccagacgtg aaacccaaca gacccctgat cgtaattctg agcactgtcg
      481 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
      541 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttga
      601 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg
      661 tcttgctcgt ctcgctggcc ggcgctgctg tcgactacgc catcatggcg acggcgcctt
      721 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
      781 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
      841 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
      901 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
      961 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
     1021 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     1081 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     1141 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     1201 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     1261 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     1321 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     1381 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     1441 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     1501 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     1561 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc
     1621 aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt
     1681 ccggcaagct atacgcgccc taggagtgcg gttggaacgt tggcccagcc agatactccc
     1741 gatcacgagc aggacgccga tgatttgaag cgcactcagc gtctgatcca agaacaacca
     1801 tcctagcaac acggcggtcc ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc
     1861 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     1921 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     1981 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     2041 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     2101 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
     2161 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
     2221 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
     2281 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
     2341 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
     2401 catcacgagc aataaacccg ccggcaacgc ccgcagcatc gcgaccgtca tcggtgagaa
     2461 gttcggcagg tattgggtgg tgacaatgta ggtgctgccc caaatggcag gtgctatcgc
     2521 tgtgaacaat aaatcgggcg tgcgtaatga catgcgatta caagacctcc gctacggcga
     2581 tgggtttttg agagttgcag gcgccgcgat gaccgcaccc tgacttggac tggccctgcc
     2641 tc
//
LOCUS       DAJTYI010000438         1503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601087
            SAMEA8900481-rid15816103.guided.259, whole genome shotgun sequence.
ACCESSION   DAJTYI010000438 DAJTYI010000000
VERSION     DAJTYI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900481
            Sequence Read Archive: ERR6004846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477760.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477760.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,288
            CDSs (total)                      :: 4,225
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 354
            CDSs (without protein)            :: 354
            Pseudo Genes (ambiguous residues) :: 0 of 354
            Pseudo Genes (frameshifted)       :: 183 of 354
            Pseudo Genes (incomplete)         :: 86 of 354
            Pseudo Genes (internal stop)      :: 124 of 354
            Pseudo Genes (multiple problems)  :: 37 of 354
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900481-rid15816103.guided.259"
                     /serovar="S. flexneri 3a"
                     /isolate="601087"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-11-17"
     gene            <1..492
                     /locus_tag="OO564_004286"
     CDS             <1..492
                     /locus_tag="OO564_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6655149.1"
                     /translation="GTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSV
                     ELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNT
                     VYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMP
                     GKP"
     gene            522..1226
                     /locus_tag="OO564_004287"
                     /pseudo
     CDS             522..1226
                     /locus_tag="OO564_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
     gene            complement(1333..1503)
                     /locus_tag="OO564_004288"
                     /pseudo
     CDS             complement(1333..1503)
                     /locus_tag="OO564_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          312 a          446 c          458 g          287 t
ORIGIN      
        1 ggcaccgtca ccgcctcgct gatgctgcgc aagctcggca gctacccgcg ccagaacgga
       61 ctggccgtgg ccctgcgcga gctgggccgg atcgagcgca cgctgttcat cctggactgg
      121 ctgcaaagtg ttgaactgcg ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc
      181 aactcgctgg ccagggcggt gttcttcaac cgccttgggg aaatcaggga tcggagcttc
      241 gagcagcagc gctaccgggc cagcggcctc aacctggtga cggcggctat cgtgctgtgg
      301 aacacggtgt acctggaacg cgccacccag gggttggtcg aggccggcaa gccggtggac
      361 ggcgagctgc tgcaattcct gtcgccgctg ggctgggagc acatcaacct aaccggcgat
      421 tacgtctggc ggcagagccg cagactggaa gacgggaagt ttcggccctt acggatgccc
      481 ggaaaacctt agcgtacgat tttttccgaa ttctgcgggc tcccctagaa gacattgctg
      541 atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc
      601 tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca
      661 tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc
      721 tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg
      781 cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc
      841 acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg
      901 gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg
      961 tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca
     1021 gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg
     1081 cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg
     1141 cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca
     1201 agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac
     1261 atcaatctca ccggagaata tcgctggcca aagccttagc gtaggattcc gccccttccc
     1321 gcaaacgacc ccaaacagga aacgcagctg aaacgggaag ctcaacaccc actgacgcat
     1381 gggttgttca ggcagtactt catcaaccag caaggcggca ctttcggcca tccgccgcgc
     1441 cccacagctc gggcagaaac cgcgacgctt acagctgaaa gcgaccaggt gctcggcgtg
     1501 gca
//
